LOCUS       DASUEX010000001         2742 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_916402, whole genome shotgun sequence.
ACCESSION   DASUEX010000001 DASUEX010000000
VERSION     DASUEX010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2742
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_916402"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="VFV61_00005"
     CDS             complement(<1..245)
                     /locus_tag="VFV61_00005"
                     /inference="COORDINATES: protein motif:HMM:NF012407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEX4898879.1"
                     /translation="MKLKITILEGDGIGPEVTREAVRVLRAIAACHGHEFNFEQKLIG
                     GAAIRQSGSPLPTTTLDACLAGDAVLLGAVGLPEFDS"
     gene            complement(284..1501)
                     /locus_tag="VFV61_00010"
     CDS             complement(284..1501)
                     /locus_tag="VFV61_00010"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: protein motif:HMM:NF002086.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEX4898880.1"
                     /translation="MPKERIAVFDTTLRDGEQSPGCSMNLAEKVRLAHQLESLGVDVV
                     EAGFPIASEGDFAAVKAVASECREVTVAALCRTAEQDVLRAAAALEPAAHPRIHTFVA
                     TSDLHLECKLKKTRAEVIEMTRLAVALALEHAEDVEFSAEDATRSDVDYLCEVFATAI
                     DAGATIINVPDTVGYTMPSEFSRLIRRIREQVVGERRVVISVHCHNDLGLAVANSLAA
                     IEAGARQVECTINGIGERAGNAALEEIVMALKVRADQLPFETAIQTQELYPTSQLLSA
                     TIGCPVQPNKAIVGRNAFAHEAGIHQHGVISNPLCYEIMTPDSVGVPANSLVLGKHSG
                     RHALALRYEDLGYSMEPAEIDTIYERFTSLADRKKRIYDQDLIALLQLGPVAGERLAG
                     EGASGTGRWAQAR"
     gene            1721..2623
                     /locus_tag="VFV61_00015"
     CDS             1721..2623
                     /locus_tag="VFV61_00015"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX4898881.1"
                     /translation="MDINQLEVLIAVAQEKSFSRAAKRLHRTQPAISQAIRRLETEIG
                     EPLFDRSSKDGTLTATGRVLFGHAQQMLNLRHSAHAAIKELKGLQRGKLGLSANEYTV
                     MYLLPILPVLRARHPHIKIEVKRSLASRISSEILARETEIGIVSFKPNDPSVASVPVL
                     TDELALIVPLNHPLAAKEVVSIRELGAESFIAHNVPSPYREKVVRTFEKYRTPLNISL
                     EMPTLEAIKRFVEGGMGVALIPKLAAQAEIARGQVASLTVREMKLERRLYLVYRKGAT
                     LSHAARAFLGIARELQKKRNADIS"
BASE COUNT          589 a          771 c          776 g          606 t
ORIGIN      
        1 aacgaatcga attccggcaa gccaaccgcg ccaagcagta ccgcgtcacc ggcgagacat
       61 gcatccaagg tggttgtcgg caacggcgag ccgctctgcc tgattgccgc gccgccaatc
      121 agtttctgct caaagttgaa ctcatgacca tggcacgcgg caatggcgcg caggactcgc
      181 acggcctcgc gcgttacttc cggtccgatg ccatcgcctt caagtatcgt gatcttaagt
      241 ttcattgtcg tggttactca gggcataact ggttcagaca gtgttagcgg gcttgcgccc
      301 atctgccggt tccgctggca ccctcaccag ccagtctttc gccggctact gggccaagct
      361 gaagcagcgc gattaggtcc tgatcgtaga tccgcttctt gcggtcagcg agcgaggtaa
      421 agcgctcgta gatagtgtca atctctgccg gttccattga atagccgagg tcttcatagc
      481 gcagcgccaa tgcatgccgt ccggaatgct tgcctaatac caggctattt gccggcacac
      541 ccactgagtc gggtgtcatg atctcatagc agagcgggtt gctgatcaca ccgtgctgat
      601 gtattccggc ttcatgcgcg aatgcgttgc gtcccacgat tgctttgttt ggctgcaccg
      661 ggcagccaat ggtcgccgaa agtagttggc tggtcggata gagttcctga gtctgaatgg
      721 ccgtttcgaa aggtaattga tcggcccgca ctttcagggc catcacgatc tcttccagcg
      781 ctgcattgcc ggcgcgttcg cctatgccat tgatcgtgca ttccacctgg cgagcaccgg
      841 cctcgatcgc ggccagactg ttagcaacgg ccaatccgag atcattgtgg cagtgcacgc
      901 tgataacgac cctgcgttcg ccgaccactt gctcacggat gcggcgtatc agacgggaaa
      961 actcagacgg catcgtatag ccaacggtat cgggaacatt gatgatggtg gcgccagcgt
     1021 cgatggcggt ggcgaacact tcacacaggt agtcaacgtc gcttctggtc gcatcttccg
     1081 cggagaattc cacatcctcg gcgtgttcaa gagcgagcgc caccgcgagc cgagtcattt
     1141 caattacctc agcccgcgtc ttcttcagtt tgcattcaag atgaaggtct gaagtggcga
     1201 cgaacgtatg aatgcgcgga tgcgctgcgg gttccaacgc tgcggcggct cttaagacat
     1261 cctgctcggc agtgcggcaa agcgcggcca cagtaacctc gcggcattcg ctcgccacgg
     1321 cctttactgc cgcaaagtcg ccttcagaag caatcggaaa ccctgcctca acaacgtcga
     1381 cgcccagact ctccaactgg tgagccaggc gaaccttttc tgcgagattc attgagcagc
     1441 cgggcgattg ttcgccgtca cgcagcgtgg tatcaaacac ggcaattcgt tctttaggca
     1501 ttttccacct ccgcaagagt ccgcatcgta gggactggat cggaaatagt caaagttata
     1561 atgtctatga caacataatc atgcgtgata agataacctg caccataggt tccaataagc
     1621 tgcggcatcg caaacgttgt ttgcgagttg gggggaggct ggctccgcgc gagttgtcca
     1681 caaactcaag acttgcgtta cagggcggta aggttctgca atggacataa accagcttga
     1741 ggtgctgata gcggtcgccc aggagaagag tttctctcgc gctgcgaaac gactccaccg
     1801 cacgcagccg gctatcagcc aggctattcg ccggctggaa acggaaatag gtgaaccgct
     1861 tttcgatcgc tcctcgaagg acgggacgct gacggcgaca ggccgcgtgc tgtttggtca
     1921 cgcgcagcag atgctgaacc tgcggcatag cgcccacgcc gcaatcaaag aattgaaagg
     1981 attgcagcgc ggcaagctgg gtctcagcgc caatgaatac accgtgatgt atttgttgcc
     2041 tatcctgccg gtgcttcgcg ctcgtcaccc acacatcaag attgaggtta aacgaagtct
     2101 ggcgagtcgc atctcttctg aaatcctcgc ccgcgaaacg gagatcggaa tagtctcttt
     2161 taagccaaac gacccttcgg tggcctcggt ccccgtgcta accgatgaac tggcgctgat
     2221 cgtgccgctc aatcatccgc tggcggcgaa ggaggttgtt tcgatacgcg aactcggcgc
     2281 agagtctttt atcgcgcaca atgtgccctc gccgtatcga gaaaaagttg tgcgaacttt
     2341 tgaaaagtat cggacgccgc tgaacatttc gctggagatg cctaccctcg aagcgattaa
     2401 gcgattcgtt gaaggcggca tgggcgtggc gttgattcca aaattggcgg cgcaggcaga
     2461 gattgctcgt gggcaggtcg cctccctaac ggtacgtgag atgaaactgg agcgtcgctt
     2521 gtatttggtt tatcggaagg gcgcgactct gtcacatgcg gcacgtgcat ttctcggaat
     2581 cgccagggag ctacagaaga aacgaaatgc ggacatcagc tagtccccat ccccagaaag
     2641 tgttcaacgt ggtggttgtt tactttttaa tccgacgatt ctcgttgcga atggggcgct
     2701 cggtgctgaa ccgggagcga gtgaccgcgc tgccctctca ag
//
LOCUS       DASUEX010000002         3415 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_801993, whole genome shotgun sequence.
ACCESSION   DASUEX010000002 DASUEX010000000
VERSION     DASUEX010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3415
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_801993"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1509)
                     /locus_tag="VFV61_00020"
     CDS             complement(<1..1509)
                     /locus_tag="VFV61_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4898882.1"
                     /translation="MAKELIVSVNGREKKIAIIENDQVTEFYIERGEENQGIVGNIYK
                     GRVMRVLPGMQSAFVDVGLERDAFLYVSDFFDEEEEFERIVMDKAKQGEVDASKAAAE
                     QIALGRVEREHRIEATQERAEPLRETEATPAVEATVESAETARRRSRRGGRRRGRGKD
                     ATTETSYAGTAPLPEEDDSETPLALPDPSIQRIVDDEETATNGEMFKDARLQERIFDQ
                     IHAVEFNFEDDFRTAEVGSILPTSSYRDDAFQRVADDEDSEAPQITMRAPTPIEAHVS
                     SFVDSAPESILHSASFQRISDEEEPLATMGVAEPEEDFEEEAETEKHGFAARAVEKIV
                     SVAKGKRASKRSPTTRVTKPRASTKRAATTRKAIAEDAPSETDVTAEEPFEDGEARMT
                     ASRTRGEFATRRGGRRRRRPTGSKKAAGTADDVEEQTDAGEQAATEVVEAPELQASPE
                     PAAAPAPAPRERSQEKRSPRGGGGRRDRQQQPTITDLLDEGQEILVQIAKEPI"
     gene            1914..2111
                     /locus_tag="VFV61_00025"
     CDS             1914..2111
                     /locus_tag="VFV61_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4898883.1"
                     /translation="MRNSRPGAHLLYLKCERFREPAHVLKPESSLRGTLDSLELSAKP
                     AAAEVFKMSRRVRRPENSRFA"
     gene            complement(2334..>3415)
                     /locus_tag="VFV61_00030"
     CDS             complement(2334..>3415)
                     /locus_tag="VFV61_00030"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEX4898884.1"
                     /translation="WLLAFMAIGIVAFGTVQIRYAQPLEAYWVKQLIGLGIALVAMFA
                     VAFTDYRRLLNIAPAFYVFGLILLVLVLIPGIGLKINGQRAWIKVPGIGQFQPSEFVK
                     VTTAMMLARYFGKHRLEALRVKEMIVGGLILALPIGLILMEPDVGSVLTYIPILAVVL
                     FLSAIKMRLVIAAVVIAVVTLPAAYWVGVKTHLIKGYQQERINVILDPEKADRRGFGY
                     NTWQSMLTVGEGGLFGSEAGSQHSQSSLKFLPEPHTDFIFAVTAGNTGFIGCILVLSA
                     YAILLSRLVTGARRAPDRMGMLVIMAIVGGLTFQIFINVGMELGILPVIGVPLPLMSA
                     GLASLIATFIAIGFAISVQLRRFVN"
BASE COUNT          706 a          969 c          946 g          794 t
ORIGIN      
        1 gattggctct ttggcaattt gcacgaggat ctcctgacct tcgtcaagca gatcagtgat
       61 cgtcggttgc tgttggcgat ctcttcttcc gccgccgccg cgcgggctgc gtttttcctg
      121 acttcgttca cgaggagcag gtgcgggagc cgccgccggt tcagggctgg cttgtagttc
      181 cggagcttct accacttctg ttgctgcttg ctcgccagca tcagtctgct cctcgacgtc
      241 gtccgcggtt cccgcagcct ttttggatcc tgttgggcga cgccggcggc gtccaccgcg
      301 gcgcgtggca aactcgccgc gagttcgact cgccgtcatg cgcgcttcac catcttcaaa
      361 aggttcttcg gcggtgacat ccgtttcgct gggagcatct tccgcaatag ctttccgcgt
      421 cgtcgccgct ctcttagtgc ttgcgcgtgg cttggtgacc cgagtggtcg gactcctctt
      481 cgaagctcgc ttgcctttgg cgacgctgac aatcttttcg acagcccggg cggcgaagcc
      541 atgtttctcc gtttcagcct cctcctcgaa atcttcttcc ggctcggcga cgcccatggt
      601 cgccagcggc tcttcctcgt cgctgatgcg ttggaatgaa gcgctgtgca aaatgctttc
      661 cggggcgctg tcgacaaagc tcgaaacgtg agcttcgatc ggcgtcggag ctcgcatcgt
      721 aatttgcggc gcctctgaat cttcatcatc tgcgacgcgc tggaaagcgt cgtcccgata
      781 actggaagtc ggcaagatcg atcccacttc agcagtacgg aagtcgtctt cgaaattgaa
      841 ttcaacggca tgaatctgat cgaaaatacg ctcctgcaat cgtgcgtctt tgaacatctc
      901 gccgttagtc gcggtttcct catcgtccac gatgcgctga attgaaggat ccggcagagc
      961 gagtggcgtt tctgaatcat cctcttctgg cagcggtgcg gttccggcgt agctggtttc
     1021 agttgtggcg tctttaccgc gtccgcgacg tcgcccgccg cggcggctgc ggcgacgcgc
     1081 agtctcagca ctttcaactg ttgcttccac cgccggagtt gcttcagttt cgcgcagcgg
     1141 ttcggcacgt tcctgagtcg cttcaatgcg atgttcgcgt tcgacgcggc ccagggcgat
     1201 ctgttcagcg gcagccttgc tggcgtcgac ttcgccttgt ttcgccttgt ccatgacgat
     1261 ccgttcaaac tcttcctctt cgtcaaagaa gtcggaaaca taaaggaagg cgtcgcgctc
     1321 caggccgaca tcgacgaaag ccgattgcat gccgggaagt actcgcatta cgcggccctt
     1381 gtaaatattg ccgacgatgc cttgattctc ttcgccgcgt tcgatgtaga actcggtaac
     1441 ctgatcgttc tcgatgatcg cgatcttctt ttcgcgtccg ttgacgctga cgatcaattc
     1501 ttttgccatg ttgtaatttg tctctcctgg aaagtcgcag gcagtagcgc tggttggttg
     1561 accgcgaccg cgcgaacgat cgttacgatc gttcggggtg acggtgccgg cgatatctgc
     1621 acttggaact tccttgtgta attgtgagtt ggcgaggtgc cgctgccgtt cagcttgaaa
     1681 gcgaggcgcg gcccgggcca gagatgagcg agctcaaaac cgatctcgct gctacttaat
     1741 gtgttgaata tttgcagcgg ttgcgacaag tctgatgaca gattgtcagc cggccaactg
     1801 cgagcttgag gcggcgcggg ctcgaattgt ttcgagcggc tacttactcc gtcgataacg
     1861 cgacggtcct ttgcactatt caaccgtaaa ccctaactac tgtttcgcta ccaatgcgaa
     1921 actcacgacc gggagcacat cttctgtatt tgaagtgcga gcggttccgg gaaccggctc
     1981 atgtgctcaa acctgagtcg tctttgcgcg gcacgcttga ttccctcgag ttatcagcga
     2041 aaccagcggc ggcggaagtt ttcaaaatga gtcgtcgcgt gcgacgacca gaaaattcgc
     2101 gtttcgcctg agcaggcgca cgaaaaactt ttcttgttct ctcgtgcaag gtgattgtcg
     2161 gtcacttact gtaccgaaat gaaaaacttc cggcaggcga actgtggctg agtatagctg
     2221 aatgaagcca aaacgcgaaa ctatttggtt ttgcgatgct aatgcgtcgg gcttattcgc
     2281 aggttaggaa agggcctgcc ccgctcaaat accctttcga catcttaacg tggtcaattc
     2341 acgaaccgtc gcaattgcac gctgatggcg aagccgatcg cgatgaaggt tgcgatcagg
     2401 gaagcgagac cggcgctcat taagggcaag ggaacaccaa tcaccggaag gattcccagc
     2461 tccatcccga cgttgatgaa aatctggaag gtcaaaccgc caacgatcgc catgatcacc
     2521 agcatgccca tgcgatcggg ggcacggcgg gcaccggtga caagtcgcga tagcaggatc
     2581 gcataagccg acaaaacgag aatgcagccg atgaagcctg tgttacccgc cgtcacggcg
     2641 aagatgaaat cagtgtgtgg ctcgggcaga aacttgagac tactctgcga atgctgagac
     2701 cccgcttccg agccaaacag gccgccttcg ccaactgtca acattgattg ccaggtgttg
     2761 taaccaaaac cacggcggtc agccttctcg ggatcgagaa ttacgttgat gcgttcctgc
     2821 tgataacctt tgatcagatg agtttttact cccacccagt aggcggcggg cagtgtcacc
     2881 actgcgatca cgacagcggc gatcactaaa cgcatcttta tcgccgacaa gaaaagcacg
     2941 accgccagaa ttggaatgta ggtaaggaca gagccgacat caggctccat cagaatcaag
     3001 ccgatcggca gggccaggat caatccgccc acaatcatct ctttaactcg gagcgcctcg
     3061 aggcgatgtt tgccgaagta gcgcgccagc atcattgcgg tcgtcacttt tacaaactcg
     3121 gagggttgaa attgtccgat gccgggcacc ttaatccagg cacgttgccc gttgattttt
     3181 aagccgatgc cgggaatcaa gaccagcacg agcagaatca gcccaaacac gtagaacgcc
     3241 ggggcaatat tcaggagacg gcgatagtcg gtaaaggcca cggcaaacat cgccacgagg
     3301 gcaatcccga ggccgatcag ttgtttcacc caatatgctt ccagtggctg cgcatagcga
     3361 atctggactg tgccaaaggc gacgattccg atcgccatga aggccagcaa ccaat
//
LOCUS       DASUEX010000003         2562 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_802340, whole genome shotgun sequence.
ACCESSION   DASUEX010000003 DASUEX010000000
VERSION     DASUEX010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_802340"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            442..708
                     /locus_tag="VFV61_00035"
     CDS             442..708
                     /locus_tag="VFV61_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4898885.1"
                     /translation="MPDEQRVANNSQTIVVDADEFTFETLEQENGQATVVRFELDDPR
                     YHAGDVLLVLSGSEIHFHGMIGSLAEGWAVATDRRGSLLAATVQ"
     gene            complement(731..1693)
                     /locus_tag="VFV61_00040"
     CDS             complement(731..1693)
                     /locus_tag="VFV61_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4898886.1"
                     /translation="MRIRKKAVALAALVITLCVGHSGTAPANAQTRIVSGDETRSGAR
                     PVTIPVGIRVKGLKPEPELQMVDLSVSEDGDPQTILSVRAIGNNSPLTLAVLIQDDLV
                     QSVANEMKPIGEFIRRLPKNSRVLVGYLRTGSLQVRQKFTTDLEKAAKALRSPLGVAT
                     AGPSSPYIEVVDAIKRFDAQPSGRRAILLVSDGLDISRGIDSSSATQSIDLQRAINDA
                     QRRGIAVYAFYSPSVATPNAILTANAQSSLQRLADETGGRAFFQGTGAPVSFDPFLKN
                     LTSSLETQIALTYLSTHLDKGFHRIKIRSATPGVELEHPAGYLR"
     gene            1861..>2562
                     /locus_tag="VFV61_00045"
     CDS             1861..>2562
                     /locus_tag="VFV61_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4898887.1"
                     /translation="MTQIAKGKLEAADQRLAIILKWAPWLSFLFISLGAPLPLLFFYL
                     RADSTETAAIFLLLALAALGAGVVIGALVLILFMLYRRGWYRRLRDRLASDGITASEV
                     TWFRSELTSEERQSLGEIQKTNPLLADAYQETLASRLTATRIVARSKRELLRVTRRIN
                     SARAIIDADTSGLVADLEADRLQLESLRDESTVRLAEAKARLQMIEAAASRSLSQTET
                     DLMLSRLSAAQSQLPL"
BASE COUNT          560 a          705 c          681 g          616 t
ORIGIN      
        1 ccaagccaat tgtcctttgg ggttttgatt cgctggggac ccgcagcggt tttggcaaca
       61 gctccactac tcaccgcttt atcctttcag tttgttttgt tttttagact cgaaatcgcg
      121 gtagggattg agcgccgccc taaggcccta cgaatctcaa gatcgggaga cgtcctaacg
      181 gcacctcacc atagcactca agcctttccc tgactgttac gattacgttg cctttccgag
      241 gcggcggacg cctttcagat tgaattggca accatcatgg cctgctgtac cgcgtaaata
      301 gatcggcaac catggctcac ttggttcttg gaatgacacg taacgcacaa accagtatca
      361 ttggtcactg tacaacgtgt gctagccgga gcttgccgta gttcccatca aatttcatat
      421 ttaatcaagc tggagataac catgcccgac gaacagcgag tcgctaacaa ttctcaaacc
      481 attgtggtgg atgccgacga attcaccttc gaaacgttgg agcaggaaaa cgggcaagcc
      541 accgtggtcc gctttgagct ggatgatccg cgttaccatg cgggggatgt tttgctagtt
      601 ctttcaggaa gcgagattca ttttcacggg atgatcggga gcctggcaga aggttgggcc
      661 gtggcgaccg atcgcagagg ctcgctcctg gccgctaccg ttcagtagtc ctccactact
      721 tattccttaa tcaccttaag taaccagccg ggtgctccaa ctccactcca ggtgtcgcag
      781 agcgaatctt gattcgatgg aatcccttgt cgagatgtgt cgagagatag gtgagggcta
      841 tttgcgtttc cagtgacgag gtaaggttct ttaagaacgg atcaaagctg actggcgcgc
      901 cggtaccttg aaagaacgcg cgaccaccgg tttcatcggc gagtctctga agcgagcttt
      961 gcgcattggc ggttaagatc gcattaggcg ttgccaccga aggggaatag aaagcgtaga
     1021 cggcgatgcc gcgtcgttgc gcgtcattga tcgctcgttg caggtcgatg ctttgagtgg
     1081 cagaactcga gtcaatgccg cgtgaaatat ccagcccgtc cgataccaat agaatggcac
     1141 gccgcccgga aggttgcgca tcgaagcgct tgatcgcatc aaccacttcg atgtacggac
     1201 tgctgggccc tgcagtggca acgcccagcg gtgaacgtag agccttcgcc gccttctcca
     1261 agtcggtggt aaatttttgc cgcacttgga gcgaacctgt tctcaagtat cccaccagca
     1321 cgcgagagtt cttcggtagg cgacgaatga attctccgat cggcttcatt tcattagcaa
     1381 cggactgaac caaatcatct tgaataagta ctgccagcgt taacggtgag ttgttaccaa
     1441 tggcccgcac cgaaagtatt gtttggggat cgccgtcctc gctcacggac aaatcaacca
     1501 tctgtaactc tggttccggt ttcagccctt tcacccgaat gcctactggt attgtaacgg
     1561 gccgggcacc actgcgcgtt tcatcccccg atacgattct cgtttgagca ttggccggcg
     1621 cggtgccgga gtgtcctaca cacaaggtga taactaaagc cgcaagcgct actgcttttt
     1681 tacgaatcct catcagtgct ccctcacgcg ccgaacatgc tcgcattata cagtcagaaa
     1741 gccggattgt tgacttgcat cccggactgg cgtcgaggct ggcgaacccg ctggttagca
     1801 gctaagatcg aaccgcgcag tttcgtatgc tggtcacacg attggttgcg tttcattttt
     1861 atgacccaaa tcgccaaagg aaaactcgaa gcagccgatc agaggcttgc aataattctg
     1921 aaatgggccc cctggctgtc atttctgttt atttcgctcg gggctccact gcccctcttg
     1981 ttcttctatc tgagagctga ttcaactgaa actgctgcca tttttcttct gctcgcactc
     2041 gctgctttgg gggccggggt agttattggc gccctggtat tgatcctgtt catgctttat
     2101 cgccgtggtt ggtatcgtag gctgcgagac cgcctcgcgt cagacggcat caccgcctcg
     2161 gaagttacct ggtttaggtc agaactgacc tcggaagaac ggcagagctt aggtgagatt
     2221 caaaaaacca acccgctgtt ggccgacgcc tatcaggaaa ctctcgcctc aaggctcacc
     2281 gccactcgca tagttgcgcg ttcgaagcgc gagctcctgc gtgtaaccag acgcatcaac
     2341 agtgcgcgcg ccataatcga cgccgataca tccggattag tagccgatct cgaggcggat
     2401 cgcctgcaat tggagagttt gagagacgag tcaaccgtcc gcttagccga ggctaaagcc
     2461 cgcttgcaga tgatcgaggc ggcggcgagc cgaagcctca gccagactga aactgacctg
     2521 atgctgagcc gcctgagcgc ggcccaatcg cagttgccgt tg
//
LOCUS       DASUEX010000004         2407 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1375027, whole genome shotgun sequence.
ACCESSION   DASUEX010000004 DASUEX010000000
VERSION     DASUEX010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1375027"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2407)
                     /locus_tag="VFV61_00050"
     CDS             complement(<1..>2407)
                     /locus_tag="VFV61_00050"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX4898888.1"
                     /translation="RELGVAFVLDGRIRRAGDQIRVTVQLLNVQNGTAVWAGQFDEKF
                     TDVLSLEDAISANVAESLVPQLSGDERRRLAKRGTNDPQAHEAYLRGRFYWNTFTEDG
                     FARAIVCYHQAIALDPHYAVAYAGIASYYNWLGSFTVLPFAECAAAAHEAASTAVAID
                     PALAEGHSALGQAILCRDFAWAQAERQLLKAIELNPAYSVPRVYYALQLGMEGRFTES
                     LREAYIARDLDPLAVISRFTVVWCLYHSRRFDEAHRIARDTLDAEPQNLMMLYGLSFV
                     LSRLGRHDEAIAAALRGVELLGKASHTLGRLGAAHAVAGNTAEAEAVLKDMDVISAHR
                     YISPYHQALVYCALGRFEESLNYLERAHELRDAKVLWLGVDPELDPLHGHPRFNDLLQ
                     KLDHRLAALPPLPAHSHTGEESIAVLPFRVLGEAAQNTGDEYLGIGLTDALISRLSNV
                     QRLIVRPTSSVLRFRGLVADPLIAGRDLGVDYIVHGSLRRLGDHLRASAQLLSVGEGV
                     TRWAEQFDEHATDVLQIEDSISERVALALLPQLSRDEKRLLSKRSTEIPEAFESYLRG
                     RYYWNTYTVAGFAKAFESYTHAIELDPDYALAYTGIADYYNWLGVFGIKSFAECSAAA
                     KEAASKAVALDATSAEAYSALGFATACHDFDWAVAEGHHRRAIEINPNYATAHHWYGF
                     HLAMCGRFDEAIREMSRARELDPLSPSIMQALAWCYYQARRFNEAITTHRNMLEAAPD
                     FSLGLATLAWTLRHVGQVDEAVEVAERSVAVSNGPLLLASLGATYAAAGRVEQAREIL
                     AQLN"
BASE COUNT          551 a          729 c          664 g          463 t
ORIGIN      
        1 gttcagctgc gcaaggattt cccttgcttg ctcaacccgt cccgcggcgg cgtaggttgc
       61 tcccagagaa gccagtagca gcgggccgtt tgagaccgcc accgatcgtt ccgcaacctc
      121 aacggcctca tcaacctgac caacgtgtcg cagcgtccag gccaaggttg ccagaccaag
      181 agaaaagtcc ggcgctgctt ccaacatgtt ccggtgagtc gtaatcgctt cattaaagcg
      241 gcgggcctgg taataacacc aggccaacgc ctgcatgatg cttggtgaaa gcggatccaa
      301 ttcacgggcg cgcgacattt cgcggatcgc ctcatcaaag cggccgcaca tcgccaggtg
      361 aaatccatac cagtgatgcg ccgtggcgta gtttggattt atttcgatcg cccggcgatg
      421 gtgaccttca gcaaccgccc agtcgaaatc gtgacaggcg gtcgcaaatc cgagcgcgct
      481 atacgcctcg gcggaagtcg catcgagggc gacggctttg ctcgccgcct ctttggctgc
      541 ggccgaacat tcagcgaacg acttgatccc aaaaacgccc agccaattgt agtagtccgc
      601 gattccggtg taagccagcg cgtaatcagg atcgagttcg atcgcgtgcg tataactctc
      661 aaaagccttg gcaaacccgg ccaccgtgta cgtgttccag tagtaacgac cgcggagata
      721 cgattcgaag gcctccggaa tctccgtgct gcgcttcgat agcagtcgct tttcatctcg
      781 cgacaactgc ggcagcagcg ccagcgctac cctctcggag atggaatcct cgatctgcaa
      841 gacgtcggtg gcgtgttcat caaattgttc ggcccaacgc gtcacccctt cacccacgct
      901 caacaactgt gctgacgcgc gcaggtgatc acccagacga cgcaggctac catggacgat
      961 gtaatcaacg ccgaggtccc gtccggcaat caaaggatct gccaccagac cgcgaaagcg
     1021 cagcacgctg ctggtcggtc tcacgatcaa ccgttgcaca ttactgagac ggctgatcag
     1081 agcgtcggtc agaccgattc ccaaatactc atctcccgta ttctgcgcag cctcgcccag
     1141 cacgcgaaac ggcagtacgg caatcgattc ctcgccagta tgagaatgcg cgggcagcgg
     1201 tggcaaagct gcaaggcggt gatccagttt ttgcagcaaa tcattgaaac ggggatgccc
     1261 atgcaaggga tcgagttccg ggtcgacgcc cagccagagc actttcgcat ctcgcaactc
     1321 gtgcgcgcgc tcaaggtaat ttagtgattc ttcaaaccgt ccgagcgcgc aataaaccag
     1381 agcctgatgg tagggagaga tgtaacgatg cgcggaaatc acatccatat ctttgagtac
     1441 ggcttcggct tcggcggtat tgccggcgac ggcgtgagcc gcaccgagcc ggcccaaagt
     1501 gtgactggct ttccccaaca gctcgacgcc gcgcagcgcc gccgcgattg cttcatcgtg
     1561 tcgacccaga cggctgagaa caaaactcaa cccgtaaagc atcatcaggt tttgcggttc
     1621 ggcatcaaga gtgtcacgcg caatccgatg agcctcatca aagcggcgtg agtgataaag
     1681 gcaccagacc actgtgaaac gagaaatgac tgcgagcgga tccaggtcac gcgcgatgta
     1741 agcctcgcgc aaactttcgg tgaaccgtcc ttccattccc agttgcagcg cgtaataaac
     1801 tcgcggcact gagtaggcag ggttcagttc gatcgctttc aacaattggc gctcggcttg
     1861 tgcccaggca aaatctcgac aaaggatcgc ctggcccaaa gccgaatgtc cttcggcaag
     1921 cgcgggatcg attgctactg ccgttgaggc tgcctcgtga gctgcggcag cacactcagc
     1981 aaacggcaga actgtaaaac tccccagcca gttgtagtaa gaagcgatgc cggcataggc
     2041 gaccgcataa tgcggatcaa gagcgatggc ctggtggtaa caaacaatgg cgcgcgcaaa
     2101 tccgtcttca gtaaacgtgt tccagtagaa gcgaccgcgc aggtaagctt catgagcctg
     2161 cggatcattc gtgccgcgct tggcgagtcg ccggcgctca tcgccgctca gttgtggcac
     2221 cagtgattcc gcaacgttcg cggaaatcgc gtcttcaaga ctgagaacgt cagtaaactt
     2281 ctcatcaaac tgtccggccc acaccgcagt gccgttctga acgttcagca gttgtaccgt
     2341 aacgcgaatt tgatcccccg cacgccggat gcgaccgtcg agcacgaaag cgacgccgag
     2401 ttcccgc
//
LOCUS       DASUEX010000005         3377 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1604145, whole genome shotgun sequence.
ACCESSION   DASUEX010000005 DASUEX010000000
VERSION     DASUEX010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3377
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1604145"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..1630)
                     /gene="aroE"
                     /locus_tag="VFV61_00055"
     CDS             complement(95..1630)
                     /gene="aroE"
                     /locus_tag="VFV61_00055"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEX4898889.1"
                     /translation="MNTENKLRVCVPVCASTITAVKEALAHACAVADIVELRLDCLLP
                     AELERVTELQPLLASLEIPVIVTLRPCEQGGSSEFDLATRSRFWTSWSGSDLFYDLEL
                     DLLEVGQWPKKTKSHHLICSHHDFSKVPSNLEAIYDRMVATPARTIKLAVHAQDVTDC
                     IPMFQLLARARQQQREMIPIAMGPAGLATRILGPARGAFLTYAALDQKSATATGQPTV
                     SELKTLYRIDTINQETEITGLIGLPLMHSVSPQMHNAALGAAGVNGVYIPFPVHDVGA
                     FIARMVHPRTREIDWNIRGLSVTAPYKSTVMDALDWIEPTAADLGAINTIVVDGNQLR
                     GYNTDILAFIQTLVASFGELPNSTCAVIGAGGAARAVIWGLKQTATNVTVFGRSEVQA
                     KTLARELDVDSRPFVDAQFGEFDLVINATTLGTAGELESQTPAGAEQLSGARLACDLV
                     YNPEETLFLREARKAGCSTLGGLPMLVQQASEQFKLWTGKEAPAQVMSQAAQQALKAL
                     SGD"
     gene            complement(1715..2239)
                     /locus_tag="VFV61_00060"
     CDS             complement(1715..2239)
                     /locus_tag="VFV61_00060"
                     /inference="COORDINATES: protein motif:HMM:NF024637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX4898890.1"
                     /translation="MFCPKCAAQNLDGAHFCRVCGANISLVPQALNGQMLQPSVPADE
                     MTRAEKRALKRAPTLDNAFRNTFMGIAFLLVTLALSFSDMGRGWWFWMFIPAFSMMGT
                     GIAQYIRFREHQRSLPGFVPAAQPIRAAAAAPVFPARGTGELLAPPPSVTEGTTRHLG
                     VEAPTKVLNNEPSQ"
     gene            complement(2261..3112)
                     /gene="rlmB"
                     /locus_tag="VFV61_00065"
     CDS             complement(2261..3112)
                     /gene="rlmB"
                     /locus_tag="VFV61_00065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEX4898891.1"
                     /translation="MANQRRLRTQKHFREDPGAPASASASERTNRSSSASDKVYGLNP
                     VLEVLRAGRRPLESVTIAEGARHERLSELLQLAKQARVPVHRVPRISLDRIDGAINHQ
                     GVIARVAAAHYRDADELLDALTAKVQSNQPPLVLGLDGVEDPRNFGAILRTAECAGVD
                     GVFIPERRAVGLTDTVAKAAAGAVEYVPIARVTNLVRLIEQLKERNIWVVGAAAEATA
                     NYSEWNWQLPTAIFLGSEGSGLHRLVKERCDSLVRIPVLGHLESLNVSVVAGILLYEA
                     LRQRASG"
     gene            complement(3115..>3377)
                     /locus_tag="VFV61_00070"
     CDS             complement(3115..>3377)
                     /locus_tag="VFV61_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4898892.1"
                     /translation="NFASLLNLICTRSPAARHDDSYLSLRQTLDVIWPSGQRTASGGW
                     RRERPGKYSIEAVTESSNVNQFTRYSRLRSFLLKRPNSENEI"
BASE COUNT          753 a          964 c          918 g          742 t
ORIGIN      
        1 ctctccgagc cgccgctgtc cctctttccc gatcccgttg aagagaatct gacggctgta
       61 gcgttcctga atttgtgtcg ctgggttttc caaatcaatc accagatagt gccttgagag
      121 cttgttgtgc ggcttgcgac atcacttgcg ccggcgcctc tttcccggtc cataacttga
      181 actgctcact tgcctgctgc accagcatgg gcaagccacc gagagtactg cagccagcct
      241 tacgagcttc acgcaagaaa agcgtctctt ccggattgta aaccagatcg caggccagac
      301 gtgcgccgct taattgctca gccccagcag gtgtctgcga ctccagttct ccagcagttc
      361 ccaaagtggt cgcgttgatc accaggtcga actcaccaaa ttgggcgtca acaaagggtc
      421 gagaatcgac atcgagttct cgcgctaagg tcttagcctg cacctcactt cgtccaaaca
      481 cagtgacatt cgttgccgtt tgcttcaatc cccagataac tgctctggcg gcgccgccgg
      541 ccccgataac agcgcaggtg gaattcggca attcaccgaa ggacgcaacg agggtttgaa
      601 tgaatgccag aatatcggtg ttgtagccgc ggagctgatt gccatcgacg acaattgtat
      661 tgatggcccc gaggtccgct gcggttggtt caatccagtc aagcgcatcc atgacggtgc
      721 tcttgtacgg cgctgtaaca ctgaggccgc gaatgttcca gtcgatttcg cgcgtacgcg
      781 gatgaaccat acgcgcgatg aacgcgccaa cgtcgtgaac tgggaagggt atgtaaacgc
      841 catttacccc cgccgctccc agcgctgcgt tgtgcatctg cggcgaaact gaatgcatta
      901 gcggcaggcc gatcaagcca gtgatctctg tctcctgatt aatcgtgtca atccgataga
      961 gcgtcttcag ctccgagacg gtgggctgac ctgtcgcggt agcacttttt tgatccaggg
     1021 ctgcgtaagt caggaacgca ccgcgtgcgg gcccgagaat tcgtgtcgcc agaccggcag
     1081 gccccatggc gataggtatc atctctcgct gctgctgccg ggcacgagcc agcaactgaa
     1141 acatggggat acagtcagta acgtcctggg cgtgaactgc gagcttaata gtgcgggcag
     1201 gagtggccac cattcgatcg tagattgctt cgaggttaga cggaaccttc gaaaaatcat
     1261 gatgcgaaca aatcaaatga tgagacttcg ttttcttggg ccactgcccg acttctaaca
     1321 gatcgagttc gagatcatag aacaagtctg aaccagacca ggacgtccag aagcgactac
     1381 gagtagcaag atcgaactca ctacttccac cttgctcaca cggtctgagc gtgacaatca
     1441 cgggaatttc cagggaagct aacaaaggct gcagttcagt tactcgctca agctcggcgg
     1501 gtagaaggca gtcgagtctg agttcgacaa tgtctgcgac tgcgcaagcg tgcgctaatg
     1561 cctctttcac agccgtgatc gtcgaagcac aaaccggaac gcagactcgt aatttatttt
     1621 ctgtgttcat caaaaagagc aatctactcc aatcgcaagg tcaagaagcg acactgcttc
     1681 gcgaaccgca ttcgttgttc caactgagca gcgcctactg cgatggttcg ttattaagaa
     1741 ccttggtggg agcttcgacg cccaggtggc gcgtcgtgcc ttcggtgaca cttggaggtg
     1801 gcgcgagcaa ttcaccggtg ccgcgagcgg gaaagacagg tgccgcagct gcagcccgga
     1861 tcggctgcgc ggctgggaca aatccaggaa gcgatctttg atgttctcga aaacgaatgt
     1921 actgcgcgat gcctgtcccc atcattgaaa acgcaggaat aaacatccaa aaccaccaac
     1981 ctctccccat gtcagaaaat gagagggcga gggtcaccag caggaaagca atgcccatga
     2041 aggtgttccg gaaggcgttg tccagcgtcg gcgcccgttt cagagcacgc ttctcggcgc
     2101 gcgtcatctc atccgcgggc actgatggct gcagcatctg tccattcagc gcctggggca
     2161 cgagactgat gttggcgcca cagactcgac agaagtgggc gccgtctaaa ttctgagcag
     2221 cacatttggg gcagaacatg atttcgcctc cgttgggggt ttagccgctt gctctctggc
     2281 gcagcgcttc gtacagcaaa attcctgcta cgactgaaac attgagggac tcaagatgtc
     2341 ccagcaccgg aatacgaacc agcgaatcgc aacgttcctt gacgaggcgg tgtaggcctg
     2401 aaccttcgct tccgagaaag atggctgtcg gcaactgcca gttccattca gagtagtttg
     2461 cagttgcttc tgccgcggcg ccaacgaccc agatgttacg ttctttcaac tgctctatca
     2521 aacgcacaag gttagttacc cgcgcaatcg gaacatattc aaccgctccg gcggccgcct
     2581 tcgcgaccgt atccgttaac cccaccgcgc gacgttcagg aataaagacg ccgtccactc
     2641 ccgcgcactc ggccgtgcgc aaaatcgctc cgaagttccg cggatcctcg actccatcca
     2701 gacccaggac aagcggtggc tggttgcttt gcactttcgc agtcagcgcg tccagcaatt
     2761 cgtcagcgtc gcgatagtgt gccgcagcca ctcgtgctat tacgccctgg tggtttatcg
     2821 cgccgtcaat ccgatccaga ctaatccgcg gcacgcgatg aacaggcaca cgtgcctgct
     2881 ttgctagttg taaaagttca ctcagccgtt catgtcgtgc accttcagca atcgttacgc
     2941 tttcgagggg ccgacgacct gctcgcagaa cttccaagac cggattcagc ccgtagacct
     3001 tgtcgctggc gctgctgctc cgattcgttc gttcggaagc agatgcgctc gccggagcac
     3061 cgggatcttc gcggaagtgt ttttgcgtcc gcaaccgtcg ctgatttgcc atgattagat
     3121 ttcgttttcg ctattaggac gtttcagtaa aaaggagcgg agacgggaat accgagtgaa
     3181 ctggtttaca ttgctgcttt ccgtgacggc ttcaatgctg tacttacctg gacgctcacg
     3241 gcgccaaccg cccgaagctg tacgctgacc ggagggccaa attacatcca gtgtttgtcg
     3301 cagagaaaga taggagtcgt catgtcgcgc cgcaggtgat cgggtgcaaa tcaggttcag
     3361 cagcgaggcg aaatttt
//
LOCUS       DASUEX010000006         2890 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_803464, whole genome shotgun sequence.
ACCESSION   DASUEX010000006 DASUEX010000000
VERSION     DASUEX010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2890
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_803464"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..885
                     /gene="argB"
                     /locus_tag="VFV61_00075"
     CDS             <1..885
                     /gene="argB"
                     /locus_tag="VFV61_00075"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEX4898893.1"
                     /translation="NQLRLDLLREALPYIQRFQGQTFVIKLSGKATEDHNNLSSLAEE
                     LALLHQVGIHLCVVHGGGKQLSELASRMGIEQTIIDGRRVTDDATLEMAKMVFAGKIN
                     TDILSALRNRGVEAVGLSGVDGNIVHAERRPPKEVLNRETGERAHVDFGHVGDVVEIN
                     SRLLTVLLDQGYLPVVSSLGADSEGTVFNINADTIAAEIAIQLKAEKLILLSDVDGIY
                     LRAGEPETKLSRLTASEAEQLITDGRATGGMIPKLQSIAQLLRRGVHSAHIISGTNRN
                     ALLSEIFTDQGTGTMIVS"
     gene            994..1452
                     /locus_tag="VFV61_00080"
     CDS             994..1452
                     /locus_tag="VFV61_00080"
                     /inference="COORDINATES: protein motif:HMM:NF026066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEX4898894.1"
                     /translation="MDNEAIARRFAQLAALMEIRGDDPFRLRSYRNAAEAIETWPVLM
                     ADIAAHEGVAGLQNVPGVGKAIAGKIMELLERGTFDVWERLTAETPATVLDLLELPGV
                     GPKTAALLHQKFRVSSLKELKAFVASGGLDQVDGIGPKTAARLKESLKKR"
     gene            1565..2011
                     /gene="tnpA"
                     /locus_tag="VFV61_00085"
     CDS             1565..2011
                     /gene="tnpA"
                     /locus_tag="VFV61_00085"
                     /inference="COORDINATES: protein motif:HMM:NF033573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HEX4898895.1"
                     /translation="MSQSYTNLLYHLIFSTKDRRPIITLDYRPRLYDYIGGIIRGLGG
                     IPLGIGGVADHVHVLAKLRPERALSDVLRDLKANASGWMHDVFPEAKDFGWQRGYGAF
                     TVSQSNVIGVQRYIASQEAHHQRVSFRDEFIQFLKANGIEYDERYL"
     gene            2253..>2890
                     /locus_tag="VFV61_00090"
     CDS             2253..>2890
                     /locus_tag="VFV61_00090"
                     /inference="COORDINATES: protein motif:HMM:NF013292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HEX4898896.1"
                     /translation="MSKKIKRRDFIRSTAAAGVTLAAAKATVSQFPTLLIQSGVKPIV
                     IASANGNHFKNGGNETCVQKAFGMMTKGADVLDAVIAGVNIVELDPLDDSVGYGGLPN
                     ADGVVQLDSSCMHGPKKRAGAVAALEGVRTPSLVAQAVLETTDHHLIVGKGAQMFARN
                     MGFKIEDDLNTDHSRKIWLEWKRRTDPAHYLDPKERAEVWRREGLKMVAEGL"
BASE COUNT          677 a          790 c          802 g          621 t
ORIGIN      
        1 aatcaattgc gtctcgatct cctgcgcgag gcgttgccct acattcagcg tttccagggc
       61 cagacttttg ttatcaaact ctccggaaag gccaccgagg atcacaataa cctcagctcg
      121 ctggcagaag aactggccct cctgcatcag gttggcatac acctttgtgt cgtacacgga
      181 ggcggcaagc agttaagtga attggcttcg cggatgggca tcgagcaaac catcattgac
      241 ggtcgccggg taacagacga tgccaccctc gaaatggcaa agatggtttt tgccggaaag
      301 ataaacaccg acattctttc ggcgttgaga aatcgcggcg tggaagcagt agggctttcg
      361 ggcgtagacg ggaacatcgt ccacgctgaa cgccgaccac cgaaggaagt tctcaatcgc
      421 gagactggcg agcgcgctca cgttgatttt ggacacgtgg gagacgtagt tgaaatcaac
      481 tcccggctgc tgacggtttt gctcgatcag ggttatctgc cggttgtttc ttcgctcggc
      541 gctgactcgg aaggcacggt cttcaatatc aacgccgaca cgatcgctgc agaaatcgcc
      601 attcagctca aggcggaaaa actaatcctg ctttctgatg ttgatggcat ctacctgcgc
      661 gcaggtgagc cggaaaccaa gctttcccgt ttgacggcgt cagaggcaga acagttgatt
      721 actgatggtc gcgcgactgg cgggatgatc ccgaaactgc aaagcatcgc tcagctgctg
      781 cggcgcggag ttcacagcgc tcacatcatc agcggtacca atcgcaacgc attgctctcc
      841 gagattttta ccgaccaggg cactggcacg atgatcgtca gttagaaact aatcgagggg
      901 aactgaagag ccacagaaag acgaacacgg atacaatcag acctgtagca aatccgtgag
      961 aagtggcgtt aacctgcggt tgaaatccca gaaatggaca acgaagctat cgcccgacgt
     1021 tttgctcagc tcgcggcttt gatggagatt cgcggggacg atccatttcg gctgcgctct
     1081 taccggaatg ctgccgaagc gattgaaacc tggccggtct tgatggccga cattgccgcc
     1141 cacgaaggcg tggcgggtct gcaaaatgtt cccggtgtcg gcaaagcaat cgcgggcaag
     1201 atcatggaat tgctggagcg tggcaccttt gacgtgtggg agaggttgac agccgagact
     1261 ccagcgactg ttctggatct actcgaattg cccggagtcg gtccgaaaac tgcggcgctg
     1321 ctgcatcaga aatttagagt ttcttcactc aaagaactca aggcttttgt tgccagcggt
     1381 ggcttggatc aggtcgatgg cattggcccc aaaaccgctg cccgactcaa ggagagtctg
     1441 aagaaacgtt agaagtctaa ccagcctgca aagcaggcgt taagcgtaaa gcctagggcg
     1501 taagccctag gtacctaggc aacctcacaa ccaaagcctg cgaagcgggc gaaaggatcc
     1561 tcacatgtct cagtcctaca cgaatctcct ctaccatttg attttctcca cgaaagaccg
     1621 gcgcccaatt attaccctcg actatagacc ccgcctctat gactatatcg gtggaatcat
     1681 ccgaggcctg ggcggaatcc cgctcgggat aggcggagtc gccgaccatg ttcatgtact
     1741 tgcgaagttg cgtcccgaac gagcactctc ggatgtgttg cgcgacttga aagctaatgc
     1801 gagtggctgg atgcacgacg tgtttccgga agcgaaggac tttggttggc aacgaggcta
     1861 tggagcattc accgtcagcc aatcgaatgt aattggagta cagcgctaca tcgcaagcca
     1921 ggaagcacat catcaacgcg tttcattccg ggacgaattc attcaattcc taaaggcaaa
     1981 tggaattgaa tacgacgaac gctatttgtg aactgccgcc cgcctgcgcg ggctcctttt
     2041 gaactttatt caccggtacc tggggcttac gccccaggct ttacgctcta cgcctgcttt
     2101 gcaggcttat tccaattgtt cgccggtcgg attctctgtt cttcttgact cctccattgc
     2161 ctaacttcgc ttctcgtgtt tgaattgcgc cgttcgatca cttccaagtt tcacttcgac
     2221 ttcaagcaca cctccaacac ccgaggagac ccatgtctaa aaagatcaaa cgtcgcgatt
     2281 tcatccgcag cacggccgcc gccggggtca ccctggcagc agcgaaagcc actgtcagtc
     2341 agtttcccac gctgctgatt cagagcggag tgaaaccaat cgtcattgcc tcagcaaacg
     2401 gcaaccattt caagaacggt gggaacgaga cctgtgtcca gaaagccttc ggcatgatga
     2461 cgaaaggcgc cgacgttctg gatgcggtga tcgctggcgt taacatcgta gagctggatc
     2521 cactcgacga cagcgtgggc tacggcggac tgccgaatgc cgatggcgtt gtccagctgg
     2581 attcgtcctg catgcatggg ccgaagaaac gcgcgggagc cgtcgccgcg cttgagggcg
     2641 tgcgcacacc atccctcgtc gcgcaggctg tgctggagac gactgatcac catctcatcg
     2701 tcggcaaggg cgcgcagatg ttcgcgcgca acatgggatt caagatcgaa gacgacctga
     2761 acaccgatca ctcgcgtaag atttggctgg agtggaaacg ccgcaccgat ccggcgcact
     2821 acctcgatcc caaggagcgc gcagaggttt ggcgacgtga gggtctaaag atggttgcgg
     2881 aagggttgat
//
LOCUS       DASUEX010000007         5737 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_803484, whole genome shotgun sequence.
ACCESSION   DASUEX010000007 DASUEX010000000
VERSION     DASUEX010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5737
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_803484"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..747
                     /locus_tag="VFV61_00095"
     CDS             <1..747
                     /locus_tag="VFV61_00095"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX4898897.1"
                     /translation="GVSVGPDGILIVDDQFAPLADKIRAALKTLGEGKLKYVLNTHWH
                     GDHTGGNAQFGPEAPIIAHDNVRRRLATEQRIELFKSTTPPAPKEALPVITFDKSLSV
                     HFNGEEIKVIHFPQGHTDGDSIIFFTNSNVVHMGDDFFVGRFPFVDLDTGGSVQGLTK
                     NIADIIPKLAADVKIIPGPGALATVDDLKQFHNMLKETTAIVQGKMKSGKTMEQIKAE
                     GLPAEWTSWGTGFIKTDVWLEIIHRSLSKK"
     gene            complement(872..2053)
                     /locus_tag="VFV61_00100"
     CDS             complement(872..2053)
                     /locus_tag="VFV61_00100"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF003766.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEX4898898.1"
                     /translation="MAPNNLFSRMILIVLDGAGIGEMPDAPEWGDAGSDTFGHVLASR
                     EVLLPNLRRYGLGNIRPLQGVPPLAAPEGSYGRCTLRSNGKDTTTGHWEMAGIILEQA
                     FPTYPHGFPPSVIDRFIRETGVPGILGNIPASGTEIIKDLGAEHVKTGKPIVYTSADS
                     VFQIAAHEQVIPLPKLYGICETARRLLSGEHEVGRVIARPFLGEPGGFYRTENRHDYA
                     VPPPRENLLVTLAAEDLDVVCIGKIASIYDSNGVTQDLTAKNNEQSINQTIHALQQDS
                     RGLIFSNLVDFDMLYGHRRDTEGFARALEHFDSRLPEIESAMRDDDLIMITADHGNDP
                     TFPGSDHTREYAPLLVYGKSAKRGVNLGSRASLADIGQTIADNFGLQLTAGESFLAQL
                     Q"
     gene            complement(2106..2486)
                     /locus_tag="VFV61_00105"
     CDS             complement(2106..2486)
                     /locus_tag="VFV61_00105"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEX4898899.1"
                     /translation="MENKTSRIPQSRADELEQRLIDFAVRIIRLSGSLPRSPAGKHIG
                     SQILRSGTSPAPNYGEARGAESHADFVHKIRIVLKELNETCIWLRVICRSQLLKRELV
                     EQLIRENEELCRIFTASLKTARRR"
     gene            complement(2598..3824)
                     /locus_tag="VFV61_00110"
     CDS             complement(2598..3824)
                     /locus_tag="VFV61_00110"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX4898900.1"
                     /translation="MKSFCKQTFFVLTLAGLLSAGVVVLPIQGQDQLKQRPRRVGEST
                     DQSNNKNTPANSQSENSEEVDEGDVVRVDTKLVSVPAVITNSAGRPLVGLRQENFVVF
                     EDNQPQAIANFGTTEAPFEIALLLDTSGSTRDDVALIRQAANAFIDALRPSDRVAITA
                     FNAERRGNDIVAAVEVLSPLTGNRSSLQKAISNLGAANGTPFYDALDRVVRDVFREAP
                     GEEVRGRRAVVALTDGVDSASETDFATARARLLGAGIACYFIQVNTEDFVEDRLLKDC
                     ADDGRLSLSAKQLERYRRIFLPRAKSEDFSNFCQMGPFERMQISRQLYNLARREMNDL
                     SQASGGRNFAAATLQEARAAFAQVANEIGTQYSLGYYPTNKNRDGKFRAIRVELRGIK
                     EKAQVRAREGYVAPKG"
     gene            4005..4079
                     /locus_tag="VFV61_00115"
     tRNA            4005..4079
                     /locus_tag="VFV61_00115"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4037..4039,aa:Val,seq:tac)
     gene            4165..4241
                     /locus_tag="VFV61_00120"
     tRNA            4165..4241
                     /locus_tag="VFV61_00120"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4199..4201,aa:Asp,seq:gtc)
     gene            4342..5550
                     /gene="glp"
                     /locus_tag="VFV61_00125"
     CDS             4342..5550
                     /gene="glp"
                     /locus_tag="VFV61_00125"
                     /inference="COORDINATES: protein motif:HMM:NF045515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HEX4898901.1"
                     /translation="MISVAQAIQIVLEQTPTLPPETVPLTDAGVRFLAQDVIADSDLP
                     PFDRSQMDGYAVRAADTVNAPVRLRIVGESAAGDGWHQEMKSGEAVRIMTGAPLPAGA
                     DSVQQVELTRDLGPELVEIQQPVLVGRSIVNRGSEVKIGTTVLRAGEQINPAVLATLA
                     SFGYAQVQVGRRPRVAVLATGSELVAVDKQPGGDQIRDSNNYSIAGYSELAGAQVERL
                     PLAGDNIALLKRQISEAAARSDIVVTSGGVSMGVYDFTKAALQELDCEIFFERVALRP
                     GKPTVFARLPNGTLVFGLPGNPVSVSVTFNLFARTAILRLQGATQPQLIEQHAILAQD
                     VKGAEGRESYLPASLHTDTEGRLIVEALKWGGSSDFVAFTRASALLIVAAGVRKQMAG
                     SVVRVVNLPD"
     gene            complement(5534..>5737)
                     /locus_tag="VFV61_00130"
     CDS             complement(5534..>5737)
                     /locus_tag="VFV61_00130"
                     /inference="COORDINATES: protein motif:HMM:NF021998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase CarF family protein"
                     /protein_id="HEX4898902.1"
                     /translation="TRLVLGPAHHQVHHTKPFNMHYCITNGWLNPLLNKIQFFRRLEA
                     MLRRLGIEVATASEREESPSPAN"
BASE COUNT         1257 a         1544 c         1598 g         1338 t
ORIGIN      
        1 ggcgtctccg tcggccctga tgggattctg attgtcgatg atcagttcgc gccgcttgcc
       61 gacaagatta gagccgcgct gaagacattg ggtgaaggca aactaaagta tgtgctgaat
      121 actcactggc atggcgatca cactggtggc aatgctcaat tcgggcctga ggcaccgatc
      181 atcgctcatg acaatgtgcg ccggcgcctc gctactgaac agaggataga actcttcaaa
      241 agcacaacac cgcctgctcc gaaggaggcg cttccggtta tcacctttga taaatcgttg
      301 tcagtgcatt tcaacggcga agagatcaaa gtgattcact tcccgcaggg acacacggat
      361 ggcgacagca tcatcttctt cactaactca aacgtcgttc atatgggcga cgacttcttc
      421 gtggggaggt ttcctttcgt tgatcttgat accggcggca gcgtgcaggg tttgacaaag
      481 aacattgcgg acatcattcc caagctcgcg gctgatgtga agattattcc cggccctggc
      541 gcgctggcga ctgtcgacga cttaaagcag tttcacaaca tgctgaagga aacgacagcg
      601 attgtgcagg gcaagatgaa gtcgggaaaa acaatggagc aaatcaaggc tgagggttta
      661 ccagcggaat ggacaagctg gggcaccggt ttcataaaga ctgacgtttg gctcgagata
      721 attcaccgca gcctgtcgaa gaagtaacac gtaggggcgg ccatccgggg ccaaccctct
      781 ctggtattcg tctcaaacct tcgaactgca agacacagat aaggggtggc cgagggccac
      841 ccctacaact gtgcaagcct gggattcgcc tttattgaag ttgtgccagg aagctctcac
      901 ccgcggtcaa ctgcaaacca aagttgtccg caattgtttg gccgatgtct gccagagatg
      961 cgcgactccc gagattgacc ccccgctttg cggacttccc ataaaccaat aatggtgcgt
     1021 actcgcgcgt gtgatcactc ccgggaaagg ttggatcgtt tccgtgatcg gcagtgatca
     1081 tgatcagatc gtcgtcgcgc atcgccgact cgatctcggg caagcgtgag tcaaagtgtt
     1141 ccagggcccg ggcgaatcct tcggtatcgc gccggtgtcc gtacagcatg tcaaagtcaa
     1201 caaggttgga aaaaatcaaa ccgcgcgaat cttgctgtaa ggcatgaatt gtctggttga
     1261 tcgattgttc gttgttttta gctgtgaggt cttgcgtcac accgttggag tcatagattg
     1321 atgcaatctt gccaatacag acgacatcta gatcttcggc agccaaagtg accagcaaat
     1381 tctcgcgcgg cggcggcacc gcataatcgt gacggttttc cgtgcgatag aaaccaccgg
     1441 gttcgccgag gaatggccgg gcaatgacgc ggccaacctc gtgttcaccc gaaagcaggc
     1501 gccgggctgt ttcacagatt ccgtaaagct ttggcaaagg aatcacctgc tcatgcgctg
     1561 cgatttgaaa aactgaatcg gccgacgtgt agacgattgg cttaccggtt tttacgtgtt
     1621 ccgcaccaag gtccttgatg atttcggttc ccgaggccgg aatgtttccc agaattccgg
     1681 gcacgccagt ctcgcgaatg aagcgatcaa tgacgctcgg aggaaatccg tggggataag
     1741 tgggaaaggc ttgttcgaga ataatacccg ccatctccca atggcccgtg gtggtgtctt
     1801 tgccattcga gcgcaaggtg cagcggccat atgagccttc gggggctgcg agcggcggca
     1861 caccctgtaa tggccggatg tttcccagac cgtagcgacg aagatttggc aggagaactt
     1921 cgcgcgaagc gagtacgtgt ccgaaggtgt cggaaccggc atcgccccac tcgggagcgt
     1981 ctggcatctc gccgatgccg gcgccatcca ggacgattag aatcatgcgg ctgaagagat
     2041 tattgggtgc catgagttac taagaacatt ttccatatct catttgatat ttctcatttt
     2101 tcatcttacc gacggcgagc ggtttttagc gatgcggtaa agatacgaca caactcctca
     2161 ttctcgcgaa taagctgctc aaccaattcc ctcttgagta attggctgcg gcagattacg
     2221 cgcaaccaga tgcaagtttc gttcagctct ttcagaacaa ttcttatctt gtggacaaag
     2281 tctgcgtgac tctcagctcc ccgtgcctcc ccgtaatttg gtgccggaga cgtgcctgat
     2341 cgcagtatct gactgccgat gtgttttcca gccggactac ggggcagact tcctgagagc
     2401 ctaataattc gcaccgcaaa atcaatcaag cgctgctcga gttcatccgc tcgcgattga
     2461 ggaattcgtg aggttttgtt ttccatccca gtgaggaagg cattgttgtt gtttgaaaca
     2521 cgttttcgtc gccctaattg acaaatgaga attaagaaat catatatgga aaatggtttt
     2581 gtttccgccg aaaatcatta ccctttcggc gcaacgtacc cctcgcgcgc ccgtacctga
     2641 gccttctctt tgattccccg cagctctact cgaatcgcgc gaaactttcc atcgcggttc
     2701 ttattcgtcg gatagtagcc caaactgtac tgcgtcccga tctcattcgc cacctgggca
     2761 aaagcggcgc gcgcttcctg caaagttgcc gcggcaaagt ttcgcccgcc actggcttgc
     2821 gacagatcgt tcatttcgcg gcgcgcgagg ttatagagct gacggctaat ctgcatccgc
     2881 tcgaagggcc ccatctgaca gaagttacta aagtcctctg actttgcgcg cgggagaaaa
     2941 atgcgccggt agcgctcgag ctgtttcgcc gacagactca agcgcccgtc gtccgcacaa
     3001 tcttttaaga ggcggtcctc aacaaagtct tccgtgttca cctggatgaa gtagcaggcg
     3061 atcccggcgc cgagcagtct cgcgcgcgcc gtggcaaaat cggtttcact tgccgagtca
     3121 actccgtccg tcaacgcaac aaccgctcgt cggccgcgca cctcttctcc cggcgcctcg
     3181 cgaaacacat ctcgcaccac acgatcgagc gcatcataaa agggcgtccc gtttgcagcg
     3241 ccgagattgc tgatggcctt ttgtaacgaa ctccgatttc cggtcaatgg cgaaagaact
     3301 tcaacggcgg cgacgatatc attgccccga cgttcagcgt tgaaggcagt gattgccacg
     3361 cgatcactgg ggcgcagggc gtcgatgaag gcgttggctg cttgtcggat caaagcgaca
     3421 tcgtcacgcg tcgagccgga tgtgtccagc aacagcgcta tttcaaatgg tgcttcggta
     3481 gtgccgaaat tagcgatcgc ttgcggctga ttgtcctcga agacaacgaa gttctcctgg
     3541 cgcagtccca cgagcggcct tccggcgctg tttgtgatta cggccggcac cgaaactagt
     3601 ttggtgtcca cgcggacgac atcgccttcg tcaacttctt cactattctc cgactgacta
     3661 ttggcaggcg tgttcttgtt gtttgattga tctgtactct cgccaacccg gcgcgggcgt
     3721 tgtttgagtt gatcctggcc ttgaatgggc aggacaacca cgcctgcgga cagcagtccg
     3781 gcgagagtta gaacaaagaa agtttgtttg caaaacgact tcatggctgc tttcgcggat
     3841 tgtcggggag acttggattg ttgactaaac tatagccgca aatttagatg gaacaaaacc
     3901 ggaaatggca tgtagcgcaa attgcgcttc ttgacacgtt ccggcgcatt acttaggatg
     3961 acactccgac gcgcacagcg cataagaatt ggttctttaa aatcgggcac gtatgtcaat
     4021 ggtagacggc ttcccttaca aggaagaggt tgggggttcg agtccctccg cgcccaccag
     4081 acggaagatg ttagatgctg aatgctagct gctatgaagc atgcattcgt gctagcatct
     4141 accatctagt atctgtttta tcgcggagtc gtagttcagt tggttagaac gccggcctgt
     4201 cacgtcggag gtcgcgggtt cgagtcccgt cggctccgcc agttcaaagc ggttgctctt
     4261 caggtagaat gaagggcaac cgctttttcc atttccgatt gccaatggcg attgccgctt
     4321 cttgtttggt cgccagtaaa tatgatctca gtcgcgcaag ccattcagat cgtcttagag
     4381 cagacgccaa cgcttcctcc tgaaaccgtg cccctgaccg atgccggtgt ccgctttctc
     4441 gcccaagacg tgattgccga ttctgatctc ccgccttttg atcgttcaca gatggatgga
     4501 tatgcggtgc gcgccgccga caccgttaac gcgcccgtcc gcctgcggat cgttggcgaa
     4561 tcagcggctg gcgacggttg gcatcaggaa atgaaatcag gtgaagcagt gcgcatcatg
     4621 accggggctc ccctgccagc cggagccgat agcgtgcagc aggtggaact cacgcgagac
     4681 ctcggtcccg aactcgtcga gattcaacaa ccggtattgg tggggcgctc aatcgtaaac
     4741 agaggaagtg aggttaagat cgggacgact gttttgcgag caggtgaaca aatcaatccc
     4801 gcagtcttag cgacgctcgc atcttttggt tatgcccagg tgcaggtggg ccggcgtccc
     4861 cgcgtggcgg tactcgcaac cggcagcgaa ctcgttgcgg tcgacaagca accgggtggc
     4921 gatcaaatcc gcgactcaaa caactactct atcgccggct actccgaact agcaggggcg
     4981 caagtagagc gcctgccgct cgccggagac aatatcgcgc tactcaaacg gcagattagt
     5041 gaggctgccg cacgatctga cattgtagtt acttctggcg gcgtttccat gggtgtttat
     5101 gatttcacca aggccgccct gcaagagctc gactgtgaga tcttcttcga gcgcgtcgct
     5161 cttcgtcccg gtaagccgac tgtttttgca cgcctcccta acggcactct cgtttttggc
     5221 ctccccggca atcctgtttc agtttccgta acttttaatc tgttcgcacg caccgcaatt
     5281 ctgcgtttgc agggagcgac gcagccgcag ctgatcgagc agcatgcgat tctggctcag
     5341 gatgtgaagg gtgcggaggg gagggagagc tatttgccag ccagcttgca taccgacacg
     5401 gaaggcagac tgatagtcga ggctctgaaa tggggcgggt cgtcagactt cgttgcgttc
     5461 acgcgagcga gtgcgttgct gattgtcgca gcgggtgtcc ggaaacagat ggcgggaagt
     5521 gttgtaaggg tggttaattt gccggactag gactctcttc tcgttcgctg gccgtagcta
     5581 cttcgattcc cagtcggcgc aacatcgcct cgagacggcg gaagaattga atcttattca
     5641 gcagcggatt gagccagcca ttggtgatgc agtagtgcat attgaacggc ttggtgtgat
     5701 gaacctgatg atgcgccggg ccgagcacga ggcgcgt
//
LOCUS       DASUEX010000008         2395 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1146267, whole genome shotgun sequence.
ACCESSION   DASUEX010000008 DASUEX010000000
VERSION     DASUEX010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1146267"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..421
                     /locus_tag="VFV61_00135"
     CDS             <1..421
                     /locus_tag="VFV61_00135"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX4898903.1"
                     /translation="LADYEKLAPRVEATEERLRENLFGASPKASCVLAYHDTRAVAFA
                     LFYFTYSTFAGLPGLYLEDLFVKPDVRGQGVGRQLLRYLAQLALREGCWRIEWAVLHW
                     NKPAIGFYEKLGAVPMDEWAVYRLFGPALSKLADEQN"
     gene            539..754
                     /locus_tag="VFV61_00140"
     CDS             539..754
                     /locus_tag="VFV61_00140"
                     /inference="COORDINATES: protein motif:HMM:NF037605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5522 domain-containing protein"
                     /protein_id="HEX4898904.1"
                     /translation="MPNPADELDSEEAALPALEEGRDYYLEQGLMVFTATFLLRRGYC
                     CERGCRHCPYGYARQPDSNEESDAQVN"
     gene            828..>2395
                     /locus_tag="VFV61_00145"
     CDS             828..>2395
                     /locus_tag="VFV61_00145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX4898905.1"
                     /translation="MAAEAIAEQHPNLGRRLWWLIIGRLAVSLLLLAVSFWTGNTTRE
                     ELWPQALPFLFLVATLTAAYALAHRSTRTRVFQVRVQIAIDIFLVTWLVWNSYLIQSP
                     YTALYIVIIAIASLFLTPREVVITSVGCAAVFTAGALLALSGYGNHPAGETLDAAAVS
                     RAIQSVGLFDVAFFVVGLLSARLAEHQSGSDVRLKAATQSLANLRALHERIVASIRSG
                     VVTTDLQGRIYTLNAAAEEITGYKELDVRGQDASIFFGDLREHTRASLNPTDSAASGP
                     RLEGDCLTAEGMRLRLGFSISPLFSETGETTGTVIAFQDLTHIRALEETSRRQDQLAA
                     IGRMAASIAHEIRNPLAAMRGSIQMLRHEMKGDSSQAELMEIILRESDRLNRIITDFL
                     NYARPRSASHAPVEVGELLRRTFTLLRFSPEVNEKQLIEEKFSEGSFLTEADAEQLQQ
                     VFWNLARNALQAMPEGGTLTAELGRKANDRLRITFSDTGRGMSPAQVERLFEPFSSTT
                     GGTGLGLSIVYQIIR"
BASE COUNT          497 a          669 c          653 g          576 t
ORIGIN      
        1 gctggcggat tatgagaagc ttgcccctcg tgtcgaagcc actgaagaac gcttgcgcga
       61 gaatcttttt ggagcatcgc ccaaggcctc ctgtgtgctc gcatatcacg acacacgcgc
      121 agtggccttt gccctcttct attttactta ctcgaccttc gccggcttgc cgggtctata
      181 ccttgaggat ctctttgtta agccggatgt tcggggccag ggggtggggc ggcaattact
      241 ccggtacctt gcccaattgg cgctgcgaga aggctgttgg agaatcgagt gggccgtgct
      301 tcattggaac aaaccagcca tcggctttta cgagaaacta ggagcagtac cgatggatga
      361 atgggcggtc tatcgattat tcggccccgc gctttctaag ctggccgacg agcaaaactg
      421 atttgtcagt ttttccatat cttcattgtc atcacccgaa ttgaccttgc gtagtgaaac
      481 ctcagcctga ggaaaagacg agtcgaagaa ttaaggctgt tccagttggt gaacggttat
      541 gccaaaccct gcagatgaac tcgattcaga ggaagcggct ctcccagccc ttgaagaagg
      601 tcgggactat taccttgaac aaggtctgat ggtcttcacc gcaactttct tactgaggcg
      661 tggctattgt tgcgagcgtg gctgccgtca ttgtccctac ggttacgcaa gacagcctga
      721 cagcaatgaa gagtcagacg cacaagtaaa ctgaactcct catcacttca ttcggagcta
      781 acaacctttt ctccccactg ccgtgctctg tggtaggctt ggctccgatg gccgccgagg
      841 cgatagcgga acaacaccct aacctggggc gcaggctctg gtggttgatc attggccgac
      901 tggcagtctc attactgctg ttggccgttt ccttttggac cggcaatacc actcgggaag
      961 aattgtggcc gcaggccctg ccatttcttt ttctcgtcgc cactctcact gcggcttatg
     1021 cactcgcgca tcgctccacc cggacgcgcg tgtttcaggt gcgggtccag atcgccatcg
     1081 acatcttttt ggtaacctgg ctggtttgga actcttacct cattcagtcg ccttacactg
     1141 cgctctacat cgtgatcatc gcaatcgcca gcttgttcct cacgccgcga gaggtggtga
     1201 ttacatcggt cggttgcgcc gccgtcttta ctgctggcgc gcttttggct ctcagcggtt
     1261 acggtaacca cccggcaggc gagactctgg atgcggctgc agtctcgcgt gcaattcaat
     1321 ctgtgggtct gtttgatgtt gcctttttcg tcgtgggcct gctctcggct cgccttgccg
     1381 aacatcaatc gggatccgac gtccggctga aagccgcgac tcaatctctc gcgaacctgc
     1441 gcgcgctcca cgaaagaatc gttgcttcaa ttcgatccgg ggttgtgacg accgaccttc
     1501 aaggacgcat ttacaccctg aacgccgccg ccgaggagat tactggttac aaggaactcg
     1561 atgtgcgcgg ccaagatgct tcgatcttct tcggcgatct gcgggaacat acccgagctt
     1621 cactaaatcc tactgactca gcggcgagcg gaccacgcct ggaaggagat tgcctgaccg
     1681 ccgagggtat gcgactccgc cttgggttta gcatctcgcc gctcttctca gaaacgggag
     1741 agacaacggg aaccgtaatc gcatttcagg atttgacgca cattcgcgcc ctcgaagaaa
     1801 cctcgcgacg ccaggatcaa ctggccgcga ttgggcgcat ggctgcctcc attgcgcacg
     1861 agattagaaa tcccctcgcg gcgatgcgtg gatcgataca aatgctgcgg cacgagatga
     1921 aaggcgattc ttcgcaagcg gaactgatgg aaatcatttt gcgtgagtcg gatcgcctca
     1981 atcgcatcat cacggacttc ctgaattacg cgcgaccacg ctcagcgagt catgcgccgg
     2041 ttgaagtcgg cgagctgctg cgacgcacat ttacgctgct acgtttcagt ccggaggtta
     2101 atgagaagca attgattgaa gagaaatttt ccgagggaag ttttcttacc gaggctgacg
     2161 ctgaacaact gcaacaagtg ttttggaacc tcgcgcgcaa cgctttgcag gcaatgcccg
     2221 agggcggcac tttgaccgcg gagctggggc gcaaagcgaa cgaccggctg cgcataactt
     2281 tctccgacac gggacgaggg atgtcaccgg ctcaggtaga acgtttattt gaacccttct
     2341 cttcaacaac cggcggcaca ggtttgggtc tttctattgt ttatcaaatc attcg
//
LOCUS       DASUEX010000009         2393 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1927, whole genome shotgun sequence.
ACCESSION   DASUEX010000009 DASUEX010000000
VERSION     DASUEX010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1927"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..843)
                     /gene="ribD"
                     /locus_tag="VFV61_00150"
     CDS             complement(<1..843)
                     /gene="ribD"
                     /locus_tag="VFV61_00150"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEX4898906.1"
                     /translation="MPTTVKSPQFANDLTDFDRQMMTRALALAEQGTGLVSPGPLVGC
                     IIVDVDGNVAGEGFYVFEDVKHAETVALEHAGTGAVAGTAYVSLEPHAHHGRTPPCTD
                     SLITAGIRRVVAPVEDPNPKVSGRGFEHLRTAGLEVSCGLMEREAQRLNEKYFHFMKT
                     GRPFVHLKLATSLDGKIGTRSGDSRWITGELARVRVQELRHEYDAILVGAGTATADNP
                     LLTDRSGLPRRRPLVRVLLDDRLLLGPQSQLVKTATDFPVLVFTSSHSDANRRSELEK
                     SGVEV"
     gene            complement(915..1727)
                     /locus_tag="VFV61_00155"
     CDS             complement(915..1727)
                     /locus_tag="VFV61_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4898907.1"
                     /translation="MKMYKLFGSGLLIASLLANALPVLAATHSEELTEIEKQQASEMA
                     IQFTKRLAETSDLSLLVKDLYLNDSVERFKRDRSKRLEGSSDPDVYFVPGLDYNSRLL
                     TDGSTEDWYRFYIAANNFLFFGFMSGIKNYSGDGKDFKPTDMYPSSVIKLLDSNPNLA
                     NMILRKGSSRPVSSVDEMKKATATLEQAAAMMRKKRNGKALLNINAGELIKLMKEDEL
                     FRPQLEIVADEVFGFPQGTRLVYISTPLGFRLTLVKSDNRLKIVWAEPYALD"
     gene            complement(1747..>2393)
                     /gene="ftsY"
                     /locus_tag="VFV61_00160"
     CDS             complement(1747..>2393)
                     /gene="ftsY"
                     /locus_tag="VFV61_00160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEX4898908.1"
                     /translation="VASETNTPENVLPYVMMIVGVNGVGKTTTIGKLAQRIKAEGNDV
                     LICAADTFRAAASDQLAIWAERTGVPLIQQKQGTDPAAVLFDSLKAARARGSDVLIVD
                     TAGRLHNKANLMAELEKMKRVAGREVPGAPHETLLVVDAVTGQNGLEQARQFLKVAGV
                     TGIVLTKLDGTAKGGIAVAIASELNLPIRYAGIGEKVDDLVVFDPELYVNGLFE"
BASE COUNT          545 a          662 c          603 g          583 t
ORIGIN      
        1 gacttccacg cccgactttt ccaattcaga tcgtctattg gcgtccgaat gcgagctcgt
       61 aaagacgagc acgggaaaat ctgttgcggt ctttacgagc tgtgattgag gcccgaggag
      121 caaacgatcg tccagcagca ctcgcaccaa gggccttctg cgaggcaatc cagaacgatc
      181 agttaataaa ggattgtctg ccgtcgcggt gccagcgccg acaagtattg cgtcgtattc
      241 gtgccgtaac tcctgcaccc gcacgcgcgc gagttcgccg gtgatccagc gtgagtcgcc
      301 agagcgggtg ccgattttcc cgtcgagcga agtagcaagc ttaagatgaa cgaacggccg
      361 gccggttttc atgaagtgaa aatacttctc attcagccgc tgcgcttcac gctccattaa
      421 tccgcaactc acttccaatc ctgcggtgcg caggtgttca aatccgcgcc cagagacttt
      481 gggatttgga tcttcgaccg gcgcaacaac gcgccgaata cccgcagtga ttagcgagtc
      541 agtgcacggc ggcgtgcggc catgatgggc gtgtggttcg agggaaacat aagcggtgcc
      601 cgcaacagct ccggttcccg cgtgttccag cgcaacggtc tcggcatgct tcacgtcttc
      661 gaagacatag aagccctcgc ccgcgacatt tccatcgacg tctacgatta tgcaaccaac
      721 gagcgggcct ggactgacaa gaccggtgcc ctgttccgca agagcaagtg cccgcgtcat
      781 catctgacga tcgaagtccg tcagatcgtt ggcaaactgc ggagatttaa ctgttgtggg
      841 catcaagcta tggtggacga attttgaacg aggcactcga acgaaagttc gacaaaccat
      901 cctctcaagc agtgctaatc aagggcgtat ggctcagccc atacaatctt taaccggtta
      961 tctgatttga cgagtgttag gcgaaaacca agtggcgtgc tgatatacac gaggcgcgtg
     1021 ccttgaggaa aaccgaatac ctcatcggct acgatttcaa gctgcggtct aaacagctcg
     1081 tcctccttca tcagtttgat caactccccg gcattaatgt ttagaagcgc cttgccattt
     1141 cgcttcttgc gcatcatggc tgctgcttgt tcgagtgtgg cagtagcctt tttcatttcg
     1201 tcgaccgagc tgaccggcct cgaactgccc ttccgcaata tcatgtttgc tagatttgga
     1261 ttgctatcga gcaatttgat aacactcgaa ggatacatgt ctgtcggctt aaagtcctta
     1321 ccatcacccg agtagttctt aatacctgac atgaaaccga agaaaaggaa attgtttgct
     1381 gctatgtaga agcggtacca gtcttcggta ctaccgtcgg tcaataaacg agagttgtaa
     1441 tcaagacccg gcacgaagta gacgtcggga tctgaacttc cttccaatct ctttgatcta
     1501 tctctcttga aacgctcgac agaatcattt aggtaaaggt ctttgacaag aagactaagg
     1561 tctgaggttt ctgcaagccg tttagtgaat tgtattgcca tctcgctggc ttgctgtttc
     1621 tcaatttccg taagttcctc actatgagtt gcggctaaca cgggtagcgc attagcgagc
     1681 agggacgcga ttagcaagcc tgacccaaaa agcttataca tcttcatcta actgcggtaa
     1741 ggagtctcac tcgaacaagc cgtttacgta caactcggga tcgaaaacaa ccaggtcatc
     1801 aactttctcg ccaattcctg cgtagcggat cgggagattt agctcgctcg caatggctac
     1861 cgcgattcca cccttcgcgg tcccgtcgag ttttgtcagc acgatgccgg tcaccccggc
     1921 gacttttaag aactggcggg cttgctccag cccattttgt cccgtaacag catcgactac
     1981 gaggagcgtc tcatgtggtg cgcccgggac ttctcgcccc gcgacgcgtt tcattttttc
     2041 caactctgcc attaggttcg ccttgttgtg caacctaccc gctgtgtcaa caatcagcac
     2101 atcagagccg cgagcccgag cggctttcaa cgagtcgaac aaaaccgctg ccggatcagt
     2161 tccctgtttc tgttgaatca atggcactcc agttcgttcg gcccagatcg ccagttgatc
     2221 cgaagcggca gctcgaaagg tgtcggcggc gcagatcaag acgtcgttac cctcggcttt
     2281 gattcgttgc gcgagcttgc caattgtggt cgtctttccg accccgttaa cgccaacgat
     2341 catcatcacg taaggcagca cgttttccgg cgtgttagtt tctgaagcaa ctc
//
LOCUS       DASUEX010000010         5241 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_918652, whole genome shotgun sequence.
ACCESSION   DASUEX010000010 DASUEX010000000
VERSION     DASUEX010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5241
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_918652"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1120)
                     /locus_tag="VFV61_00165"
     CDS             complement(<1..1120)
                     /locus_tag="VFV61_00165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEX4898909.1"
                     /translation="MAETKEEISASEVEVERWDRETLKPTLIKAPETKQRFESVSLEE
                     VQRLYTPADLGNLNFSEDISFPGEFPYTRGIHPTGYRGKLWTMRQFAGFSTPEETNAR
                     FKFLLAQGQHGLSVAYDLPTLMGYDADSLLSEGEVGKCGVAVSSLADMEVLFDEIPLE
                     QVTVSQTINAPASVLLAMYLVVAEKQGADWKRISGTLQNDILKEYIAQKEWIYPIRPA
                     MKLVVDTFEFCTQHVPRYNPVSVSGYHIREAGATAIQELAFTLRDGLEYVEWGLKAGL
                     ALDSFVPRISFFFNAHNDFFEEIAKYRAARRIWAREMRDRFGSINERTLKLRFHTQTA
                     GVSLTVQQPLNNIVRVAIQALAGVLGGTQSLHTDAYDEA"
     gene            complement(1355..1771)
                     /locus_tag="VFV61_00170"
     CDS             complement(1355..1771)
                     /locus_tag="VFV61_00170"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEX4898910.1"
                     /translation="MSRVVNSYWVDERIRSQRVASRAVSPFAPVREISVELIGSRADI
                     RRRGGIIPSWVLFGMIMLATLAVCATVTVRTRTRMNAASEQYVRMRTDVQALRDSNKV
                     LKVEVEQLRSNPRAIESAARTRLNMVRDNEILVPIE"
     gene            complement(1740..3146)
                     /locus_tag="VFV61_00175"
     CDS             complement(1740..3146)
                     /locus_tag="VFV61_00175"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HEX4898911.1"
                     /translation="MAIRFGTSGWRAVIADDFTFAGVRQVTEAICAHFETSDKLQKAV
                     VIGHDTRFLGERFAAECAGVVSAHGFRTLLCNQPTPTPTISHAIRSEGAVCGINFTAS
                     HNPPEYNGMKLSTSDGAPALPEITKHVEGLIAQGGVVNESKKGTTPQSSNFETYSPRQ
                     AYLEDLKKKIRFDVISKAAGRYAFDPLWGTGRGYLDEALRMHGLEVETIHDWRDVLFG
                     GRSPEPEESHLTELRELVISKKCTLGLATDGDGDRFGVIDATGEFITPNQLIALLFDY
                     LAESRNWTGGVARSVATSHLVDRVAEARGLPVYETPVGFKFIGELINEDKIILGGEES
                     AGLSIKGHYPEKDGILACLLAAEAVAARGESLTQQLTKLYARVGRLEAGRIGVRLTGD
                     LMKSLGARLDQSPTAIGGRAIERTNRLDGVKFIFGDGSWLLMRPSGTEPLVRIYAESE
                     SATDLEVLLEQGRKFLLG"
     gene            3625..5175
                     /locus_tag="VFV61_00180"
     CDS             3625..5175
                     /locus_tag="VFV61_00180"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX4898912.1"
                     /translation="MPSSPNSSLEAESDLAFEENSLPISSMVRAVCQAAGNGPDHLYE
                     VMVKLSLMVEATAPTYGLSIWTCEVTGQAQLKWAEGLDEAELRRAERTVADTLASPIT
                     LPEIREGDSSFCFLLAKPQLDRQGAAFYGCCVRPLTDLQAKNLRALFDVTQLAHAQAA
                     AIRPAAIREQAPAGGVAASALPGMVFSSRAMTEVAKSVERIKDSDSTVLISGESGTGK
                     DLISRAIHRLSRRAGKEFVPFNCSAVPADLIESMLFGHRKGTFTGALNDHEGLIRAAE
                     NGTLFLDEIGDLPLSLQPKLLRFLQEGEIHTLGERMPRKVNVRVIAASHKNIEQLVQQ
                     KTFREDLYYRIAALTIKVPPLRERPDDVATLISHFLTHYARRNDRAIAGITWEAIRIL
                     EEYSWPGNIRELAAEIERLVLFADENGFITPEHISEHILPQRLRSREATDRADGNLEN
                     LLEDYERRVITEALKRHDCNVALTSEALGLGSRQTLYKKLKRLAIDIGEFLQEDTEPG
                     LQFRADRQ"
BASE COUNT         1220 a         1433 c         1409 g         1179 t
ORIGIN      
        1 gggcctcgtc ataagcatcg gtgtgcaagc tttgtgtgcc accgagaacg cctgccagtg
       61 cctgaatcgc tactcgcaca atattattca gcggttgttg tacggttagc gatacgccgg
      121 cggtttgcgt gtggaaccgc agtttgagag ttcgttcgtt aatggacccg aagcgatcgc
      181 gcatctctcg cgcccaaatc cggcgcgctg cgcgatattt cgcgatctcc tcaaagaaat
      241 cattgtgcgc attaaagaag aacgaaattc gcggaacaaa actgtcgagt gcaagtcccg
      301 ccttcaatcc ccattccaca tactcaagcc catcgcgcaa agtaaacgcc aattcctgaa
      361 tcgcggtcgc gcccgcttca cgaatatgat aaccgctcac tgacaccggg ttgtaacgcg
      421 gcacgtgttg cgtacaaaac tcaaacgtgt caacaacgag tttcatcgcg gggcgaatgg
      481 gatagatcca ctctttctgg gcaatgtact ctttcaaaat atcattttgc agggttccgg
      541 aaattcgttt ccagtcagcg ccctgcttct cggcgacgac gaggtacatt gcgagaagta
      601 ccgatgccgg cgcattgatc gtttgcgaaa ctgtgacctg ctcaagcggt atttcgtcga
      661 agagcacttc catgtcagcg agcgaggaaa ccgccacgcc acacttgccc acttcgccct
      721 ctgaaagtag cgaatcagcg tcgtagccca tcaatgtagg caggtcgtag gcgactgaca
      781 gaccgtgttg accctgggca agcaggaact tgaaccgcgc gttcgtttct tcgggagtgc
      841 tgaaccccgc gaactgtcgc atcgtccaaa gcttgccgcg gtaaccagtg ggatggatgc
      901 cgcgcgtgta gggaaactct ccagggaagg aaatgtcttc agaaaaattt aggttaccga
      961 gatcggctgg agtgtaaaga cgttgcacct cttcgagtga aacactttca aagcgctgct
     1021 tggtctcggg tgccttgatg agtgttggtt tcagtgtctc gcgatcccag cgctcgactt
     1081 ccacctcact cgcggaaatc tcttctttgg tttcagccat aaccctctca gttggcatga
     1141 gttgtttaga atatcgatag tagtcaaggg tatggagggt gtcaaactac attgagttga
     1201 ccttaagaag agaggggcgc ggctacgatg gcagtcatag ccgcgccagc agggcaggac
     1261 ccggttacgc gtggattcaa gcaggggaac tagcggcggc gattggcagg tctggaaatc
     1321 gtttcattgt ttcctcgcga taagctttgc cggcttattc gataggcacc aggatttcgt
     1381 tatcgcgcac catgttgagt cgggttctgg ccgctgactc aatcgcacgc ggattactac
     1441 gtaactgttc gacttcaact ttgagaactt tattgctgtc ccgcagcgct tgcacgtcag
     1501 tccgcatcct gacgtactgc tcggaagcag cattcatcct cgttcgcgtg cgcaccgtta
     1561 ctgtggcgca gacggcaagt gtggccagca tgatcatgcc aaagagcacc cacgacggga
     1621 tgatcccacc ccggcgccgt atatcagcgc gcgagccgat cagctctacg gaaatctcac
     1681 gcaccggcgc gaacggagat accgcacgcg atgctacacg ctgtgagcga attcgctcgt
     1741 caacccagta agaatttacg accctgctca agaagcacct ccaagtccgt tgccgattct
     1801 gattctgcgt aaatgcgaac gagtggctca gtgccggagg gccgcatcag cagccaggag
     1861 ccatccccaa aaatgaactt cacaccgtcc aggcgattcg tgcgctcaat agcgcgccca
     1921 cctatagccg tcggactctg atctaacctt gcacccaacg acttcatgag atcgccggta
     1981 agacgcacgc ctattcgtcc cgcttcaagg cgaccgacgc gcgcgtagag ctttgtaagc
     2041 tgctgtgtca ggctctcgcc gcgcgcagcg actgcctccg ctgccagcag gcacgcaagg
     2101 atgccatctt tctccggata gtgacctttg atcgataatc ccgccgactc ttcaccgccc
     2161 aggataatct tgtcttcatt gatcaattct ccgatgaatt tgaaaccgac aggcgtctcg
     2221 taaactggga gaccacgcgc ttctgccacg cgatctacca gatgcgacgt tgccaccgag
     2281 cgagccacgc cgcccgtcca gtttcttgac tcagccagat agtcaaagag caaggcgatt
     2341 agctggttgg gagtgatgaa ttctccagtg gcatcaatga cgccgaaccg atcgccgtcg
     2401 ccatctgttg ccaggcccaa ggtgcacttc ttcgagatga ctagttcacg cagttcagtg
     2461 agatgactct cttccggttc tggtgagcga ccgccaaaca gaacatcacg ccaatcgtgg
     2521 attgtctcga cttccaatcc atgcattctc agtgcttcat ccaggtatcc acgcccggtt
     2581 ccccagagcg ggtcgaatgc ataacgaccg gcggctttgg agatgacgtc gaagcggatc
     2641 ttcttcttca agtcttccag gtaagcttgt cgcggagagt aagtttcaaa atttgagctc
     2701 tgcggtgtcg tgcctttttt cgactcatta acgaccccgc cctgggcaat cagaccttcc
     2761 acgtgcttgg tgatctcagg tagcgccggc gctccgtcgg aggtcgaaag cttcatgccg
     2821 ttgtattccg gcgggttgtg ggaagcagtg aaattaatgc cgcaaacagc gccttcagaa
     2881 cgaatcgcgt gggaaatggt cggcgtcggc gtcggctggt tgcagagcag agtgcgaaat
     2941 ccgtgcgccg aaacaacccc ggcgcattcg gccgcaaatc tctcccccag aaaacgggta
     3001 tcgtggccga tcacaactgc cttttggagc ttgtcgctgg tctcaaaatg agcacaaatc
     3061 gcttcggtga cctgacgcac cccggcaaac gtgaaatcgt ctgcgatgac ggcgcgccaa
     3121 cctgaggttc cgaatcgaat agccatgctg ttttttaaga gcctgcttaa ccgactctct
     3181 ggaactccac cacaagatag ctggtatttc tgaaaagcct gacccaaggg aatgggcttc
     3241 tgccactgcc aatccgttgc cagtaaaaga tttagcggca aggtggccgg aatgtaacat
     3301 cggtgcttaa gagagtaaag atttttgttt gcaactgttt cgcaacgtaa cttcaacgta
     3361 tacttgaagt aagcgcgctt aatggaccaa agtggcgaac gcttggcgta cggaaagccg
     3421 agacctttcg aacggaccgc gcgacgctgt ttaattctgt tgacactcca tgccatgcgc
     3481 cactagactc acaaaacccc ggtcggaacg ccgaccatca aggtcgcagt taaggattct
     3541 cgccgaccaa ctcacgcaac atctgcaaac acgatctttc ttgcccctca ctgtgcacga
     3601 ctgacaggaa gcttaaagaa cgacatgccg agctcgccga attcaagttt ggaagccgag
     3661 agtgatctgg ctttcgagga aaacagtctt ccaattagtt cgatggtgcg tgctgtctgc
     3721 caggccgctg gcaacggccc tgatcacttg tatgaggtga tggtcaaact gagcttgatg
     3781 gttgaggcca ctgctccaac ttatgggctt tctatttgga cgtgcgaagt tacggggcaa
     3841 gcgcaactaa agtgggccga gggtcttgat gaagccgaat tgcggcgcgc cgaaagaacg
     3901 gtggctgaca ctcttgctag tccgatcacg ttacccgaga taagagaggg agattcgtcg
     3961 ttttgtttcc ttctcgcgaa gccgcaactg gatcgacaag gagctgcgtt ctacgggtgt
     4021 tgcgtccgac cgttaacaga cctccaggcg aagaatttgc gcgcactttt tgatgtcacg
     4081 caactggctc acgcacaggc cgctgcgata agaccagctg cgattcgtga acaggctccg
     4141 gcgggcggcg ttgctgcttc agcacttccg ggaatggttt tttccagcag agcgatgacg
     4201 gaggttgcga agtcggttga aaggatcaaa gactctgatt cgacggtact gatcagcggc
     4261 gaatcgggaa cgggaaaaga cctaatttcc cgggcaattc atcgtctgag ccgccgagcg
     4321 ggaaaagaat ttgttccgtt caactgttcc gccgttcccg ctgacttgat cgaaagcatg
     4381 ctcttcggtc atcgcaaagg gaccttcact ggcgccctca acgatcatga aggattaata
     4441 cgcgctgcag aaaacggcac actgtttcta gatgagatcg gcgatttgcc gctttctctg
     4501 caaccaaaac tcctgagatt cctgcaggaa ggagagatcc acacgctggg tgaacgaatg
     4561 ccccgcaagg tcaacgtccg agtgatcgca gcgagtcata agaacatcga gcagttggtc
     4621 cagcagaaaa cgttccgtga ggatctctac tatcggatcg cggctttaac gattaaggtg
     4681 ccgccgctgc gggagcgtcc tgacgacgtt gcaaccctaa tctcgcactt tctaactcat
     4741 tacgcccgtc gtaatgatcg agcgatcgcc ggaattacct gggaagcgat ccgaattctt
     4801 gaggagtatt cctggcccgg caatatacga gagttagcgg cggaaattga gaggcttgtg
     4861 ctcttcgccg atgagaatgg cttcatcact ccggagcaca ttagtgaaca catcctgccg
     4921 caaaggttgc gcagtcgcga ggctactgat cgcgcggatg ggaacctgga aaacttactc
     4981 gaggactacg agcggcgcgt tatcaccgaa gcattgaaac gacatgactg caacgtggcc
     5041 ctgacgagtg aagctttggg gttgggttcg cgtcagactc tctataagaa attaaagcgg
     5101 ctggcgatcg acatcgggga gtttctccag gaagacactg aacccggatt acagttccgg
     5161 gcggaccgcc aataagctcc tcaacaggaa ggaaatcagc actcgagatt aagtctcgag
     5221 tgcttttttc tttagctaac c
//
LOCUS       DASUEX010000011         3300 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_117106, whole genome shotgun sequence.
ACCESSION   DASUEX010000011 DASUEX010000000
VERSION     DASUEX010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3300
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117106"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            186..1115
                     /locus_tag="VFV61_00185"
     CDS             186..1115
                     /locus_tag="VFV61_00185"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX4898913.1"
                     /translation="MREKIKAIDFMYYVATGEFMEQWDRAKEGELICRMEKAIGGLPR
                     FVSIDDMLRQMDEAGVEKVFITQCKMWSYWRKWMYMDTQLEDVLQYTQRYPNRFVGLA
                     GYNPFRIKESLAEIELAVTKYDFKGVYVHIYGFDIPLNDAKMYPLFAKCIELDVPVSM
                     QVGHVLEAMPSDGARPMFLDRIACDFPDLKMIGAHTGWPWVDELISVCYKWEKVWFGI
                     DAWLPKYLSPQVVQFMNSRLGRDRCLWGTNGLPWKESLKQLCELGLKPEVERKVLREN
                     AMELFNLAPTEKQPTVVETIPQTKPAHLETALS"
     gene            1112..1945
                     /locus_tag="VFV61_00190"
     CDS             1112..1945
                     /locus_tag="VFV61_00190"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HEX4898914.1"
                     /translation="MSTETLTTVENDVAVRENLISSLLDFTGKSVLVTGGARGIGRAI
                     VSAFAHAGADVVIGDLRFEDAHDTAENISQLSHRRVVAVQADVTDREAVQRLNEETQR
                     LFGKVDVLVNCAGWDRLLPFTKTTPDLWQKILAINFLGLVNLCHVVLPHMVERKQGVI
                     VNISSDTGRVGSFGEAIYASSKAAINAFSKTLAREHARDNIRVNVVSPGLCETPLIDE
                     MRQDEFTKKILGSIVNFIPLKRLGRPEEIAPLVVFLASDAASYITGQVFSVNGGLNMV
                     G"
     gene            1987..3135
                     /locus_tag="VFV61_00195"
     CDS             1987..3135
                     /locus_tag="VFV61_00195"
                     /inference="COORDINATES: protein motif:HMM:NF017828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HEX4898915.1"
                     /translation="MITSSSDCEDIISDVNFSTVREWKEQHPGAKAIAYFPVYAPVEL
                     IHAAAMLPVGLSGAGDQLDIQYADARFGSFICSIVKTTLELGLTNHLTPFDGVLFSSI
                     CDSARNLCFVMKRNFPDKYIDFLHLPHNPNSKSSTDFLVTEYRRLLEQLESLNGRQVS
                     DENLNEAVTLYNANRALTRQLYDERARQPQLLRTSELYALVRVGNFLPVAQHTELLQR
                     TISDLPTRVGKQRDSIRIIVEGSFCEQPPLDLIKILEEAGCYIVDDDFVLGPRWFLED
                     VPVNGDPLRALAESYSERAVYSSVRHDARKPRHKELIEKVRRRKADAVILLIAKFCEP
                     ALFDYVLFKEELEKEGIPHLLMEFEEKMFTFERLRTEVETFVESLLFD"
BASE COUNT          766 a          833 c          925 g          776 t
ORIGIN      
        1 ccgcagccgc tgaataagtt cgcagttgtc cctaccgtcc aacgctcccc atcttgctgt
       61 tccgagcacc attagccctg ttttgctggg aatgcgcgaa atccgctgcc agaagagggc
      121 tgaagtcccc cacgaagacc gaggtatgca agttgccatt ttgggtacag gtcgcggagg
      181 gcgagatgag agaaaagata aaagccatcg atttcatgta ctacgtcgcc actggcgagt
      241 ttatggaaca gtgggaccgg gcgaaggaag gcgagctgat ctgccgtatg gaaaaggcga
      301 ttgggggatt gccacgattc gtttccattg acgacatgtt gcggcagatg gatgaagccg
      361 gggtggagaa agttttcatc acccaatgca agatgtggtc ttactggcgc aagtggatgt
      421 acatggacac ccaactcgag gatgttctgc agtacacgca gcgctatccc aatcgttttg
      481 ttggcctcgc cggctacaac ccgtttcgga tcaaagagag cctggcggaa attgaactcg
      541 ctgtgacgaa atatgatttc aagggcgtct atgtgcacat ctacgggttt gatatcccac
      601 tcaacgacgc gaagatgtat cccctctttg ccaagtgcat cgagctggac gtgccagttt
      661 ctatgcaggt cggtcacgtg ctggaagcga tgcccagtga cggtgcgcgc ccaatgtttc
      721 ttgaccgcat tgcctgcgac ttccctgatt tgaaaatgat tggagcgcac accgggtggc
      781 cctgggtcga tgagctgatc tctgtctgct acaagtggga gaaggtctgg tttggcatcg
      841 atgcctggct tccaaagtat ctttcaccgc aggtcgttca attcatgaat agccgcctgg
      901 gtcgtgatcg ttgcctctgg ggtaccaacg gtttgccctg gaaggagagt ctgaaacagc
      961 tttgcgagct cggtttgaaa ccggaagtgg aaaggaaagt gctgcgcgag aatgcaatgg
     1021 agttgttcaa tcttgcccca acagaaaaac aaccaactgt cgtcgagaca attccacaaa
     1081 ccaagccggc ccacctggag acagcgttgt catgagcacc gagacactta caaccgtaga
     1141 aaacgacgtc gctgtgaggg agaacctgat tagcagtctg cttgatttca ctggcaagtc
     1201 ggtactggtg accggagggg cgcggggcat cggtcgagcg atcgtgtcgg cctttgccca
     1261 cgccggtgcg gatgtggtca tcggagatct tcgttttgag gatgcgcatg atacggctga
     1321 aaacatcagc caactctctc accggcgcgt tgtggcagtg caagcggacg taacggaccg
     1381 tgaagccgtg caaaggttga acgaggaaac ccaaagacta tttgggaagg tcgatgtgct
     1441 ggtgaattgt gcgggctggg atcgattact tcctttcacc aagacgactc cagacctctg
     1501 gcaaaaaatt cttgcgatta actttcttgg cctcgtcaat ctgtgtcacg tcgtcttgcc
     1561 gcacatggtc gagcgtaaac agggtgtcat agtcaacatc agctcagaca ctggccgcgt
     1621 cggctcgttc ggcgaggcga tctacgcgag ttcgaaggcg gcgatcaacg cattctcgaa
     1681 aacgctggca cgagaacacg cgcgcgacaa catacgagtt aacgtcgtct ctccgggact
     1741 ctgtgagacg cctttaattg acgagatgcg gcaggatgag ttcacgaaga aaatattggg
     1801 atcgatagtc aacttcatac ccttgaagcg tctgggcagg cccgaagaga tcgcgccctt
     1861 ggtagtgttt cttgcttctg atgcggcgag ctatatcacc ggacaggtct ttagcgtcaa
     1921 cggcggccta aacatggttg ggtgaagcgg ggaacttacc agggcggggc caatagattg
     1981 gcaaacatga tcactagttc cagtgactgc gaagacatca tttctgatgt gaatttttcc
     2041 accgtgcgcg agtggaagga gcaacatccg ggcgctaagg ccatcgctta ctttcccgtt
     2101 tatgctcccg tcgaactgat tcacgctgcc gcgatgttgc cggtcggcct gagtggtgcg
     2161 ggcgaccagc tcgacatcca gtacgccgat gctcgcttcg gttccttcat ctgctcgatc
     2221 gttaagacaa ctctcgagct gggactcaca aatcacctga cgccatttga cggtgtgctc
     2281 ttttcctcca tttgcgattc agcgcggaat ctctgctttg tgatgaagcg taactttccg
     2341 gacaagtaca ttgattttct ccatctcccg cacaatccaa attcgaaatc cagcaccgac
     2401 tttttggtga ctgaatatcg tcgtttgctg gagcagttgg aatcactgaa tggtcgccag
     2461 gtcagcgatg agaatttgaa cgaagctgta accctttata acgcgaatcg cgcgctgacg
     2521 cgccaactct acgacgaacg cgcccgccag ccacaactgc ttcgcaccag tgagttgtac
     2581 gcgttggtac gcgtcggaaa cttcctcccg gttgcacagc acactgaatt actgcagcgc
     2641 accatttctg atttgcccac acgcgtcggc aaacagcgag attccatccg tatcatcgtg
     2701 gaaggttcgt tctgtgaaca gccgccgctc gatctaatca agattctgga agaagcgggt
     2761 tgctacatcg tcgatgacga ttttgtactc gggccgcgct ggtttctgga agatgtgcct
     2821 gtcaatggtg atccactgag agcgctggct gaaagttatt ccgagcgagc ggtgtactca
     2881 tccgtgcgtc acgatgcccg taagccgcgc cataaagaac ttattgaaaa ggttcgtcgc
     2941 cgaaaagcgg atgcggtgat tctgctcatc gctaaatttt gcgagcccgc actctttgat
     3001 tacgtgctct tcaaagaaga attggagaag gagggcattc cgcatctgct gatggagttt
     3061 gaagagaaga tgtttacttt cgagcggctg cgaactgaag tggagacctt tgttgagtcg
     3121 ttgttgtttg attgagggcg cgaagacaat gtcaacgcaa actgaatatc ggagcgaact
     3181 gcaccagcga caaaaagagt tgatggtcaa atggtatgaa cgtctcaatg cagcggccga
     3241 gacgggcagc cccccttctg tctcaatgat ggtctccggc aattgcgtcg aattgctcga
//
LOCUS       DASUEX010000012         3593 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_577196, whole genome shotgun sequence.
ACCESSION   DASUEX010000012 DASUEX010000000
VERSION     DASUEX010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3593
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_577196"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="VFV61_00200"
     CDS             complement(<1..485)
                     /locus_tag="VFV61_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4898916.1"
                     /translation="MTGTLNFGARRHHAFLPCLLLALLVIVAVVRFPPAGAQGSTPIL
                     ISEETSTRALVFDSTTREREPFTANTQVTFGGDTRRRLMIFAMHLQLEPGELPSAVVA
                     RAEDASHQIYLLPVEHVGPTPGQTWATSIVVSLDPAMGDLGDVLVGVTYHGVSSNRVR
                     VG"
     gene            708..2825
                     /locus_tag="VFV61_00205"
     CDS             708..2825
                     /locus_tag="VFV61_00205"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEX4898917.1"
                     /translation="MVAPKEFVASICFLFAAVMCVPGQSDGSVKGVAQDSNRVNVPGV
                     VLVFTNQVTSVTKLVTTGAGGTYSLQLPAGAYRVTVQSPYSAKFEKEKNYGEFAIPRD
                     RALENFIVEAGKQTTLDVVVEKQKSEPLASVPGEKPPGYAREDSVASRPQTVPDRREV
                     RDRWRLGFPEYDRYGDRGGRGRDIPFTKGRWWDPYNQSVLKGDYPIHGNNLFMVLSAT
                     STTTVEQRRAPTPSDVSSDEPISGEFFGQPEQLAASQTIQLSFELFHGNTVFKPRDWA
                     IKLSPTFSVPNYLNARENGVVNIDVRRGTNRTDTHISLEEAFGEVKLADLSRNYDFIS
                     LRAGIQPFVSDFRGFIFSDNNLGARLFGSFDNNRYQFNAAYFSMLEKDTNSGLNTFDT
                     RHQNVYIANLFRQDFIRKGYTIQTSFHFNDDRSSIEYDQNGFLVRPALIGDVRPHAVK
                     VGYVGVNGDGHLGRLNLTNSYYFAFGHDDRNPIAGRKVSIRSHMAAVEASLDKDYLRF
                     RGSVFWAQGDANPTDETGSGFDAIFDDPNFVGGQFSFWNRNGIRLTQTGVGLVQPNSI
                     LPSLRSSKTQGQANFVNPGIFIYNAGFDVEITQRLKAVVNANYLRFHRTEPLEYILLQ
                     NRIRHDIGFDYSLGVAYRPFLINNVTLTFGAATLQTGRGFRDIFTDRSRNCPANISDF
                     CEPDVISPRKMLYNLFAQLKLIF"
     gene            2850..>3593
                     /locus_tag="VFV61_00210"
     CDS             2850..>3593
                     /locus_tag="VFV61_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4898918.1"
                     /translation="MQRVSLIIAFSLVAIAVVATSNSSPTNAGRAGEIRLARPTQTIS
                     SYREPSGINQAAQAVPTEGCITCHGQIEPMHKYGPTEVYETLKDGKDAVGLTCTACHG
                     GNPTVRKTGNDPAESERAKKAAHVQARFPEEWRRDGKYTGANPERTNTLLARESWEFV
                     RFVNPGDIRVATQTCGNSGCHAIEAKNVARSMMTHGAMLWGAALYNNGGFPLKDARFG
                     ESYNERGAPQRLLQVPQPSVEERRTKGLLS"
BASE COUNT          839 a          983 c          999 g          772 t
ORIGIN      
        1 cctactcgca cacgattgct ggaaacaccg tgataggtca cgccgaccag cacgtcgccc
       61 aggtcgccca tcgccgggtc aagactaacg acaattgagg tggcccacgt ttgtccggga
      121 gttggcccga cgtgctcgac cggcaacagg taaatttggt gcgaggcatc ttcggcgcgc
      181 gccactaccg cgctcggcag ctcgccgggc tcaagctgca agtgcatggc aaagatcatc
      241 agccgacgac gcgtgtcgcc tccaaaggtg acttgcgtgt tggcagtaaa aggttcgcgc
      301 tccctcgtgg tcgaatcaaa aacgagagcg cgagtcgaag tctcttcaga aatcagaatg
      361 ggggttgagc cttgcgcccc agcgggagga aaacgaacaa cggcgacgat gactaaaagc
      421 gccaacagca ggcagggcaa aaaggcatga tgcctgcggg cgccaaaatt aagagtgcca
      481 gtcatcctcc gcaccatcgt tgttaaggtt gatctagtct cgaggcttta tcagaaacaa
      541 agactacagg cagagacaat gtatcaccaa tctttacctg ctgaatagag ttaggcccaa
      601 aggagaattt aattgcctca gcatccctcg tatgctacaa ggcaagcaac tgcgtggacg
      661 ctcgcgcttg cacattttat cgactccctg gcattcagcg cgccaaaatg gtagctccaa
      721 aagaatttgt cgccagcatc tgctttctat ttgcggccgt gatgtgcgtc ccaggccaga
      781 gcgacggatc cgtgaaaggc gtcgcccagg acagcaatcg cgtgaacgtg ccgggcgtgg
      841 tgctcgtgtt tactaaccag gtgacgagcg tcacaaagct cgttacgact ggcgcgggag
      901 ggacgtattc gcttcaactt ccagcgggcg catatcgagt cacggtgcag tctccctact
      961 cggcgaagtt tgaaaaagaa aagaactatg gcgagtttgc gatcccgcgc gaccgggcgt
     1021 tggagaactt catcgttgag gcgggaaaac aaaccacgct cgacgtggtt gtagagaagc
     1081 agaaatcgga gccgctggcg agcgttcccg gcgaaaagcc tcccggttat gcacgcgaag
     1141 acagtgtagc ttcacggccg cagaccgtgc cggatagacg tgaggttcgc gatcgctggc
     1201 ggttgggttt tcccgagtac gaccgttacg gcgatcgcgg cggtcgcgga cgtgatattc
     1261 ctttcaccaa aggtcgttgg tgggatccct acaatcaaag cgttctcaaa ggcgattacc
     1321 cgatccatgg caacaacctc ttcatggtgc tctcggcgac cagcaccact acggttgagc
     1381 aaaggcgcgc gccgactcct tccgatgtca gcagcgacga acccatcagc ggtgagtttt
     1441 ttggccaacc ggaacaactt gcggccagcc agacgatcca actgtccttt gaactatttc
     1501 acggcaacac ggttttcaag ccgcgcgatt gggcgatcaa gctttcgcct actttcagtg
     1561 ttcccaacta tctcaatgcc cgagaaaatg gagtggtcaa tatcgatgtg cggcgcggca
     1621 ctaatcgcac cgacactcat atctcgctgg aagaagcctt cggggaagtg aagcttgcgg
     1681 atctgagtcg taactacgat ttcatatcgt tgcgcgctgg gatccagccc tttgtttctg
     1741 acttccgcgg cttcatcttt tcggataaca acctgggcgc gcgacttttc ggttcttttg
     1801 acaacaaccg ctatcagttc aatgccgctt acttttcgat gctcgagaaa gacacgaaca
     1861 gcggcctgaa tacttttgat acccgtcatc agaatgtcta catcgcgaat ctttttcggc
     1921 aagacttcat tcggaagggc tacacgattc agacaagctt tcacttcaac gacgatcgta
     1981 gcagcattga atacgatcag aacggcttcc tggtgcgccc cgcgctaatc ggagacgtaa
     2041 gacctcacgc agtaaaagtt ggctacgtcg gagtgaatgg tgatggccat ctgggccgcc
     2101 tcaatctaac caactcttat tacttcgcct ttggacacga cgatcggaac ccgattgccg
     2161 gccgcaaggt cagcattagg tcgcacatgg cggcagtcga agcttcgctg gacaaagatt
     2221 acctgcgctt tcgcggttcg gtgttttggg cgcaaggcga cgcaaatccc accgacgaaa
     2281 cgggctcggg ctttgatgcg attttcgatg atccgaattt cgtgggcggc cagttttcat
     2341 tttggaatcg caacggcatt cgcttaacgc aaacaggcgt tggtttggtc cagccaaata
     2401 gcatcttgcc gagcctgcgc agcagcaaga ctcaggggca ggctaatttt gtgaatccgg
     2461 gaatctttat ctacaacgcc gggttcgacg tcgagatcac acagcgatta aaggcggtcg
     2521 tgaatgcaaa ctacctgcgg tttcatcgaa cggagccgct cgaatacatt ctgcttcaga
     2581 atcgcatacg tcacgacatt ggcttcgact atagtcttgg cgttgcctat cgaccgttcc
     2641 tgattaacaa cgtcacgctc acatttggag cggcaactct gcagactggc cgtggctttc
     2701 gcgacatctt taccgatcgc tcgcgcaact gcccggctaa catcagcgat ttttgcgagc
     2761 cagatgtcat tagtccgcgc aagatgctct acaacctgtt tgctcaattg aagttgattt
     2821 tttgagttgc gacttgagtc gaagctttta tgcaacgcgt aagtctgatc attgctttta
     2881 gcctggtagc gattgcggtc gttgcgacga gcaattcatc gcctacgaat gccggacgtg
     2941 ctggtgaaat acggctggct cgaccgacgc agactatatc gtcgtatcgc gagccgagcg
     3001 gcattaacca ggcagcgcaa gcggtgccga cggagggctg cataacttgt catggccaga
     3061 tcgagccgat gcacaaatat ggcccgactg aggtttatga aacgctgaaa gacgggaaag
     3121 acgcagttgg cttaacttgc acggcttgtc acggtggcaa tcccaccgtg cgcaaaaccg
     3181 gcaacgaccc cgccgaaagc gagcgtgcca aaaaagcggc gcatgttcag gctcgttttc
     3241 cggaggagtg gcgccgcgac ggcaaataca cgggcgccaa tcctgagcga accaacacct
     3301 tgctcgcgcg cgagagttgg gaattcgtgc gcttcgtcaa tcccggagac atccgggtgg
     3361 cgacgcaaac atgcgggaac tcaggctgtc acgcaatcga ggcaaagaat gtggctcgca
     3421 gcatgatgac gcatggcgcg atgctttggg gggccgcgct ctacaacaac ggcggttttc
     3481 ctttaaagga cgcgcgcttt ggagaaagct ataacgagcg cggggcgccg caacgcttgc
     3541 tacaagtccc gcaaccctcc gttgaggagc ggcgcacgaa aggtctgctc agt
//
LOCUS       DASUEX010000013         2372 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_4566, whole genome shotgun sequence.
ACCESSION   DASUEX010000013 DASUEX010000000
VERSION     DASUEX010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4566"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..963)
                     /locus_tag="VFV61_00215"
     CDS             complement(<1..963)
                     /locus_tag="VFV61_00215"
                     /inference="COORDINATES: protein motif:HMM:NF015056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HEX4898919.1"
                     /translation="MFSKHSRLAPSSSWLLAAASIVALCGPAAAAEIKYDPGDNVQFT
                     YCHSHPPVLRIKPGDSVVTNTKDASNDVYSTGDKTLFPKLDLSKVNPQTGPFYIEGAE
                     PGDTLVVRIDKIDFNRDWGWGGSIPYFGALAPEYKTMMITAPVPDKLFIWRIDRNRKV
                     GTLDLPNSKIGRVEVPLRPFFGTIGTAPSGKECISSLVPGSHGANMDFNEVIEGVTMY
                     FPVYERGALFMLGDGHAAQGDGEIDGAAIETSFNVRFTVNLIKGKRIGWPRLVNDKVI
                     MSIGSTRPLMDALRLACVDLINWLVEDYGYDKLEALQLLGQAAQL"
     gene            complement(1029..1961)
                     /locus_tag="VFV61_00220"
     CDS             complement(1029..1961)
                     /locus_tag="VFV61_00220"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HEX4898920.1"
                     /translation="MGIAVALGIVGIIAAMAKLCCFTVETGEYALVTEFGKPVQVITE
                     PGLGFKYPYQSVRTFDSRLFAYTPASTEFLTLEKTPVVAASTVLWRVSDPKKFFQTVF
                     DRAGAESRLGDILFAELGAAIGRSPLAAFVSIEPKAFRAEAILAEVSGKCREIALRDY
                     GIEVIDVLLRSFDFPKQNRPPLYSRMKSERGRLSMKFRSEGEEEGLKIRAAADQEKSR
                     ILSEALKLAQQHRGEGEGKAARIYAESLSKGPEFYEFVRTMQASRNLIPKGTTLMLPV
                     DSELFGLLQDSSHYDRGGENRKTIARRKKEVPMK"
BASE COUNT          450 a          726 c          681 g          515 t
ORIGIN      
        1 gagttgcgca gcctgaccca gcaactgcag cgcttcgagc ttgtcatagc catagtcctc
       61 gaccagccaa ttgataaggt cgacacaggc cagccgcagg gcatccatca acggccgggt
      121 gctgccaatc gacatgatca ccttgtcgtt gaccagccgc ggccagccga tccgtttgcc
      181 cttgatcagg ttcacggtga agcgcacgtt gaacgaggtc tcgatggcgg cgccgtcgat
      241 ctctccatca ccttgcgccg cgtggccgtc accgagcatg aacagcgcgc ccctctcgta
      301 caccgggaaa tacatggtga cgccttcaat cacctcgttg aagtccatgt tcgcgccgtg
      361 cgaacccggc accagcgaac tgatgcactc cttgcccgaa ggcgcggtac cgatggtgcc
      421 gaagaagggc cgcagcggca cctcgacgcg cccgatcttg ctattgggca gatcaagggt
      481 gccgaccttg cgattcctgt cgatgcgcca gataaacagc ttgtcaggca ccggtgccgt
      541 gatcatcatc gtcttgtatt ccggcgccag cgcgccgaag tagggaatcg atccgcccca
      601 gccccagtcc ctgttgaagt cgatcttgtc gatgcgaacg acaagcgtat ctccaggctc
      661 cgcgccctcg atgtagaacg gcccggtctg aggattgacc ttgctcagat cgagtttcgg
      721 aaagagcgtc ttgtcacccg ttgaatatac gtcgttcgag gcgtccttgg tgttcgtaac
      781 cacgctgtcg ccaggcttga tccgcagcac cggcggatgc gaatgacaat aggtgaactg
      841 aacattatcc cctggatcgt acttgatctc ggctgcggcg gccggtccac acaaagcaac
      901 aatggatgcg gcagcaagca accacgaact cgatggtgcc aacctgctgt gcttcgaaaa
      961 catgacaacc tcccgattat ggttagtgtt gctgagtcat tcggtgacaa caccgtaatt
     1021 gccttgtgtc atttcatcgg cacctccttt ttgcgtctgg caatggtctt cctgttctcg
     1081 ccgccgcggt cgtagtggct gctgtcctga agaaggccaa acaactcgga gtccaccggc
     1141 agcatcaggg tcgttccctt gggtatgagg ttgcgcgaag cctgcatggt gcgcacgaat
     1201 tcatagaatt ccggcccttt ggagagcgac tcggcgtaga ttcgcgccgc cttgccttcg
     1261 ccctccccgc ggtgctgctg ggccagcttc agcgcttcgg aaagaatgcg gcttttctcc
     1321 tggtccgccg ccgcccggat cttgagcccc tcttcttcgc cttccgagcg gaacttcatg
     1381 ctcagacgcc cgcgttcgga tttcatgcgg gagtaaagag gaggtctgtt ctgtttcggg
     1441 aagtcgaaac ttctcagcag aacgtcgatc acttcgattc cgtagtctct cagcgcgatt
     1501 tcgcggcact tgccgctcac ctcggcaagt atcgcttccg cccgaaaggc tttgggctcg
     1561 attgaaacga aggcagcgag agggcttcgc ccgatcgccg cgccgagttc cgcaaacagg
     1621 atgtcgccga gtcgcgactc cgctccggcg cggtcgaaga cggtttggaa gaacttcttg
     1681 gggtcgctga cacgccagag gactgtgctt gcagcgacga ctggcgtttt ttcgagcgtc
     1741 aggaactccg tggacgccgg ggtataggca aacaggcggc tgtcgaatgt tcggacgctt
     1801 tgatacgggt acttgaagcc aaggccgggc tcggtgatga cctgcaccgg cttgccgaac
     1861 tcggtgacga gcgcgtattc cccggtttca accgtaaagc agcatagctt cgccattgcg
     1921 gcgatgatgc cgacgattcc cagcgcgacg gcaataccca tccgtcgtcg tgtgttcgat
     1981 tgcccgccac tgccatcaac tcccgccatg acctcccccg gccatcacgc agaagatatt
     2041 acgacgtcga catttgagtt tgatcatcgc agtctgcggc cgttgtatgg acttgacaag
     2101 tggggctcga tcgcgcgcgt gttgcgccgc aacgaaaacc gggaggcaga atggtcgatg
     2161 cggatttcag agcttccccg ggagtgcttc gcaggcgcgt gcaatgaccg tcttcaagtc
     2221 atgtctgcta cagtgaatcg atagctcttt tccggacgac ctgcgccatg ccaatccttg
     2281 cctggattgt tgccgcaacg ctgcttggtg gcgttctgag cgtgattgcc gccgccatcc
     2341 tcgcgctgcg tgcagggccg aaccaactgc cc
//
LOCUS       DASUEX010000014         3817 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_921126, whole genome shotgun sequence.
ACCESSION   DASUEX010000014 DASUEX010000000
VERSION     DASUEX010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3817
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_921126"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2106
                     /locus_tag="VFV61_00225"
     CDS             <1..2106
                     /locus_tag="VFV61_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4898921.1"
                     /translation="AKYLKGLAKLKFDGLSFADLNVPLDQAPLKSLETGITYAQPIAV
                     GGDSEIIIEAGVSGGLKLYSAKDEQLFDPEVFADPISIKANQFYVGIDTRAKFSPEFT
                     ATPGDLSFGLSGDSSIGFSTYRLFEKAEAATFPLWLDALKESLANFAIPRNVDDLAGM
                     SVGSIAAVEGSGSLKVSGEVELLSAINPLASVNLPEPVGTLGVSSGGSIKVGASFEIS
                     GAFQIRVHKTGVSKATLGIYRKRGKELNVRATASIGASVAVGDFKPLEPLLNALSSNP
                     KADLEKLKEQLGEGKVDEIKKVVEAGISRKLELALALELTSQTTSTAAFLFEIDLNRL
                     EAGGREALHQALGGNLTKLPLQQENLPTGIALARTIFTEVKKQKHALKFNLLGIYNFI
                     SVSTLILKGRVMFEPATGELVITDTATASRISASSLNFAADGEKLRKVLAESVLISAA
                     YRCSNLVTQPPRLKIAHSYFELHTKTDQKVLKNNLDVFEAVGLMKHDEKKGTVASAKQ
                     FGRTTLYCETGYNNEVVESLFLSAGKPRPQTDYEPAGRKALELLVESGEEAEGRRLPV
                     TDPQLWKQMTELGQANFSTIDKLRNLPANTLGAITSDYSVIMWWARSMREMGEALAQV
                     REFISKNPGSDPLAEPFKGLRKKLGSKLKDVASKTKSEFGDPWGLVAMDQASGQRATA
                     AARLNSSTLAILRERNAPL"
     gene            2389..>3817
                     /locus_tag="VFV61_00230"
     CDS             2389..>3817
                     /locus_tag="VFV61_00230"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEX4898922.1"
                     /translation="MRNKRVATLATTCSLVLILAGAVSAQTFRGNILGTITDANGAVV
                     PAANVTARNEATGLERTAVTDEAGNYSLSELQIGKYQLTITKSGFQKLVINQVTVEVA
                     AERRVDASLEVAGTSDQVIIAPSAQAETTSNTIGGLITTRAAEDLPVNGRDFTKFLVL
                     VPGATGDPSGATDSPGSFGLFSANGNRGRANNYLLDGTDMNDGYRNLPAINEAGVFGT
                     PATILPIEAISEAAILSNFEAEYGRNSGAVVNIVTKSGTNEFHGSLFEFFRNNALDAR
                     NFFNPEPDPQTAFRNNQFGGSLGGPIVRNKTFFFFAYEGQRERVGLNSTARVPDPREI
                     AALGGATNPVIARLLARNPWPTPNRPLPLFDDTGTPNLFVTTRALNDVDSLIGKIDHS
                     FNEHNQLTGRYFFGNSDQSFPLAILAGNVLPGYNTVTPTRVNLISLSYLKLLSSTKVN
                     EVRFGYNRFDEGFFPEDGDFDPNSIG"
BASE COUNT          915 a         1038 c         1030 g          834 t
ORIGIN      
        1 gccaaatatc ttaagggcct cgcgaagcta aagttcgacg gcttgagttt tgccgacctt
       61 aacgtccccc tcgatcaggc gccactcaag agtctggaaa cgggaatcac ctatgcacaa
      121 ccgatcgccg tcggcggtga ctcagaaata atcattgagg ccggcgtctc aggtggcttg
      181 aagctttact cggcgaagga cgagcagctg ttcgatccgg aagtattcgc ggatccaatc
      241 tcgatcaagg ccaatcagtt ttatgtaggc atcgatacga gggcgaaatt ctctcctgag
      301 ttcaccgcaa cgccgggcga tctgagtttt gggctgagcg gcgacagcag cattggcttc
      361 tccacctatc gtttattcga gaaagcggaa gccgcaacgt ttccgctctg gctcgacgcc
      421 ctaaaagaga gcctcgccaa cttcgcgata ccgcgcaatg ttgatgacct tgccgggatg
      481 agcgttggga gtatcgccgc agttgaaggc agtggcagtt taaaagtgtc tggcgaagtg
      541 gagttgctaa gcgcgattaa cccgctggct tcggtaaacc tgcccgaacc tgtaggaacg
      601 cttggagtga gttcaggagg ttcgatcaaa gtgggggcgt cgttcgagat ttccggtgcc
      661 tttcaaattc gtgttcataa gactggagtt agcaaggcca ccctcggtat ctaccgtaaa
      721 cgaggcaaag agctcaacgt cagggccaca gcctcaatcg gcgcgtcggt tgcagttggt
      781 gatttcaaac cgctcgaacc actgttgaat gcactcagca gcaatcccaa agctgatctg
      841 gaaaaactca aggaacaact tggcgaaggt aaggtcgatg agatcaagaa ggtggttgaa
      901 gcgggtatct cgcgcaagct ggaactcgct ttggcccttg agctcacatc gcagacaacc
      961 tcgacggctg cctttttgtt tgaaattgat ttgaaccgct tagaggcagg cggtcgcgag
     1021 gcgcttcatc aagctctggg tggcaatctc acgaaattgc cgttgcagca agagaacctc
     1081 ccgacgggca tcgctttggc ccgcactatc ttcaccgaag ttaagaagca gaaacatgct
     1141 ctcaaattca acttgttggg catttataac ttcatctcgg tcagcacgct gattctaaag
     1201 ggtcgtgtga tgttcgagcc ggccaccggc gagctcgtga tcaccgatac ggcgactgct
     1261 tcgagaatct cggcttcgag tctcaacttc gcggccgatg gcgagaagtt gagaaaggtt
     1321 ctggcagaga gcgttttgat cagtgcggcg tatcgttgca gcaatttggt tacccagccg
     1381 ccgcggttaa agattgccca cagttatttc gaacttcata ccaagaccga tcaaaaggtg
     1441 ttgaagaata acctcgatgt atttgaagct gtggggttga tgaagcatga cgaaaagaag
     1501 ggcacggttg cgagcgccaa gcaatttggc cgcaccacgc tttattgcga aactggttat
     1561 aacaacgagg tagtagagag cctgttcctt agtgcaggaa agccgcggcc acagactgac
     1621 tacgaacccg cgggtcgaaa agcattggag ctcttagtgg aaagtggcga ggaagcagaa
     1681 ggacgacgtc ttcctgttac cgacccacag ctttggaaac agatgaccga gcttggtcag
     1741 gcaaactttt caacgataga caagttacga aatctgccag cgaatacgct cggagccatt
     1801 acgagcgact acagtgtaat catgtggtgg gcgcgctcga tgagggagat gggagaggcg
     1861 ctggcgcagg tacgcgagtt catcagcaag aacccaggct ccgatcctct cgccgaaccg
     1921 ttcaaagggc tgcgcaagaa gttgggctcg aagttaaaag acgtggccag caaaacgaag
     1981 agtgagtttg gcgatccctg ggggttagtg gcgatggatc aggcatcggg tcaacgggcg
     2041 actgcggcgg cgcgtcttaa cagctcaacc ttagcgatcc tgcgcgaacg caacgccccg
     2101 ttatgaggta agtacgacct gcggtagcgg gccggtatca ctaagaaagc cacgcggacg
     2161 ctacccagtt gggcgaacaa ctttccacat tggcgatctg atcttctcat tgacagtgtt
     2221 ccgtcggggg atataatccg tcgcatcggc ggtccaccac tagtctccac aatcagctat
     2281 ttgattacat agtcactgcc acaaagctga ggaggtaatt cgtccatgct aagcacgacg
     2341 aggcaatccc tgacctcaac tcccgacttg tccgcaacaa tccgttggtt gcgaaacaaa
     2401 agagtcgcga cccttgcaac tacctgctcg ctggtcctga tacttgccgg ggccgtctct
     2461 gcacaaactt ttcgcggcaa cattctgggc accattaccg atgccaatgg cgcagttgtt
     2521 cctgcagcca atgtaacggc tcggaatgaa gccacagggc tggaacgaac ggccgtcacc
     2581 gacgaagcgg gaaactacag tctgagcgaa ctccagatcg gcaagtacca gctaacgata
     2641 accaagtcgg gattccaaaa gctcgttatc aaccaggtga ctgttgaggt tgccgccgag
     2701 cgccgcgttg atgcgagtct ggaagtggcg ggaacttcag accaggtaat catcgctccc
     2761 agcgctcagg ccgaaaccac ctccaatacc atcggcggtc tcatcaccac cagagccgca
     2821 gaagatctcc ccgtgaatgg ccgcgacttc accaagtttc tggtacttgt gcccggcgcg
     2881 accggtgatc cctcgggagc caccgattca cccggctcgt ttggattatt tagtgctaat
     2941 ggcaatcgcg gtcgggccaa caactatctc ctcgacggca ccgacatgaa cgatgggtac
     3001 cgtaatctgc cggctatcaa cgaagcggga gtgtttggca cgccggccac cattcttccc
     3061 atcgaggcga tctctgaagc cgcaattctc tcaaactttg aagcggagta cggacgaaac
     3121 tcaggtgcag tagttaacat tgtaaccaag tccggcacca atgaatttca cggttcgctt
     3181 ttcgagttct ttcggaataa cgctctcgac gctcgaaatt tcttcaaccc cgagcctgat
     3241 ccgcaaaccg catttcgcaa caaccagttt ggcggctcac tgggcggacc tattgtgcgc
     3301 aacaagacat ttttcttttt cgcctacgaa ggtcagcggg aacgtgtcgg attgaattcg
     3361 accgcgcgtg tgcctgatcc gcgcgagatt gcggcgcttg ggggcgcgac taatccagtc
     3421 atcgcccggc tgctcgcgcg taatccctgg cccacgccca atcgtccgct tcccttgttt
     3481 gacgacaccg gcactcccaa tctcttcgtc accacccgcg ctctaaatga cgtcgacagc
     3541 ctgatcggga agatcgatca ctcattcaac gagcataatc agctaaccgg tcgctatttc
     3601 ttcggtaaca gcgaccagtc attcccgctc gcgatcctgg ccggtaacgt cctgcccggc
     3661 tataacaccg tgacgccaac acgcgtaaat ctcatttcgc tctcgtacct gaaactcctg
     3721 tcgagcacta aagttaacga agtcaggttt ggctataacc gtttcgacga aggtttcttt
     3781 cctgaagatg gcgattttga tccaaacagt attgggc
//
LOCUS       DASUEX010000015         3218 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1494118, whole genome shotgun sequence.
ACCESSION   DASUEX010000015 DASUEX010000000
VERSION     DASUEX010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3218
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1494118"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1988
                     /locus_tag="VFV61_00235"
     CDS             <1..1988
                     /locus_tag="VFV61_00235"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEX4898923.1"
                     /translation="ITFNVVPNELTRVAGVEVRGNKIYTEKQLRDELKTVIGAPFSRA
                     QARFDGDRVLALYSREGYANAQVELSVVELPRKGDEEQVRLIYTITCRSDTGERNDTC
                     VNEGDKVFVNRIIVNGVTGDAKTQQRKRNAIMRAIPLQEGDVLRADWVAESERELYLT
                     DAYRQVIIHPEPAGETASGFKKKDLIIDVEEKKPLVMDYGGGFSTDTGALGLFELSNV
                     NLMNKLRHGAMRLRVSRQQQLIRLEYIDPKFKRYNKRQFAPLALTVQYQRDSTVTRFF
                     RSAIDRGTFGIVQRLDEDGNPIDELGARVKEPTINRLTIALETQRILDQKTHTIVFAR
                     YSYEDVRLFNLQSLLIRPILQPDRAVRLSRIGASLVRDTRERCERGLLGAVRDPDSAE
                     SALPGEVCRYNQVDATRGYFFSVDYSVALRQLGGNVSFNKLQASYRTYHKINRLRGTV
                     LAGNATLGLANLFNPRDRDGNGQIDEIDRTLPISERFFSGGSTTLRGFGFEEAGPRQA
                     IIPEGTFRDQDGDIVELNPFTVPVGGNALAVVNLEARIPLTKALQVVPFYDGGNVFRR
                     VGDLFGKKDSTPVPAGDVVAAINAANLRAHWSNTVGLGFRIQTPVGGALAVDYGFLLN
                     PPEFLIPQRGPGGGFDGTPAIFRLNRSQVHFRFTQTF"
     gene            2074..2832
                     /locus_tag="VFV61_00240"
     CDS             2074..2832
                     /locus_tag="VFV61_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4898924.1"
                     /translation="MINSDSNVSIKSRVIARVLSLIIISLFALTPFPIFAASNINVFS
                     PSPFLRVTASPAQEVVDRMVATVNAGVRTDLITYSDLVWQLALQPNTPLVSPNSQDLN
                     RALRLVIDQRLILQEAEKLPTIAPTAEEVKNARDGLSKAFASAAEFQQRLMLVGLTSE
                     KLDEILEQRVRIEKYLDFRFRNFVLISQKEITDYYKDVYAPRVRGRGQIVPTLEEARA
                     EIEGILTEAKIESDTDAFLDSARERAEIVMLSGV"
     gene            2926..>3218
                     /locus_tag="VFV61_00245"
     CDS             2926..>3218
                     /locus_tag="VFV61_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011367009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX4898925.1"
                     /translation="MVRTKKTATVGLALSGGAARGIAHVGVLQALVENQIPIDFIAGT
                     SAGALVGGAAASGMPLSELETLGRNLRWRDIGRMTMSRSGVQSNERLEQYLRAR"
BASE COUNT          752 a          815 c          917 g          734 t
ORIGIN      
        1 tcatcacctt caacgtcgta cccaatgaat tgactcgcgt tgccggagta gaagtgcggg
       61 gcaataagat ttacaccgag aagcaactgc gtgacgaatt gaagactgtg attggcgcac
      121 ccttctcgcg ggcgcaagcg cgcttcgatg gcgaccgcgt gctcgctttg tattcgcgag
      181 agggttatgc caacgctcag gttgagcttt cggtggtgga actgccgcgc aaaggcgacg
      241 aagaacaggt acgtttgatc tacacgatta cttgtcgctc cgatacgggc gagcgtaacg
      301 atacttgcgt caatgaaggc gacaaagttt ttgtcaatcg cattatcgtc aatggcgtaa
      361 ccggagacgc aaaaacacaa cagcgcaaac gaaacgcaat catgcgcgcg attccgcttc
      421 aggaaggcga cgtgttgcgt gcggattggg tggcggaaag cgaacgcgag ctttacctca
      481 ccgacgccta tcgccaggtg atcattcacc ccgaaccggc cggcgaaacc gcctcaggtt
      541 tcaaaaagaa ggatttgatt atcgatgtcg aagaaaagaa accgctcgtg atggactacg
      601 gcggcggctt ttctacggac acgggcgcgc tcgggctctt tgagctctcc aatgtgaacc
      661 tgatgaacaa actgcggcac ggcgccatgc gtttgcgggt tagccgacaa cagcagttga
      721 tccggctgga atacattgat cctaaattca agcgctacaa caagcgacaa ttcgcaccgc
      781 tcgctttgac cgtgcagtat caacgcgact ctaccgtgac ccgtttcttt cgttcagcca
      841 ttgatcgggg caccttcgga atcgttcaac gattggatga ggatggcaat ccaattgatg
      901 aattgggcgc gcgcgttaaa gagccgacca ttaatcgttt gacaattgcc ttagaaacgc
      961 aacgcatcct cgatcagaaa actcacacga ttgtcttcgc tcgttacagc tatgaagacg
     1021 tgcgcctctt caatctgcag agtcttctga ttaggccaat cctgcaacct gatcgagccg
     1081 tgcggctgtc tcgcattggg gcatcactcg tgcgtgacac tcgcgagagg tgcgagcgcg
     1141 gtttgctggg cgcggttcgc gatccggata gtgctgaatc cgccctgcca ggtgaagttt
     1201 gtcgctataa ccaggttgac gcgacacgcg gatacttctt cagtgtggac tattcagtgg
     1261 ccttgcggca gttgggtggc aatgtctcgt tcaataagtt gcaggcgtcc taccgcacct
     1321 accacaagat caatcgtttg cgaggaactg ttttggcggg taacgcgacc ctcggactgg
     1381 caaacctatt caatccgcgc gatcgcgatg gcaacggcca gatcgacgag atagatcgaa
     1441 cgttgcccat tagcgaaaga ttcttctcgg gtggctcaac tacactgcgc gggtttggat
     1501 ttgaagaagc aggaccgcgg caggcgataa ttccggaagg cacttttcgt gatcaggatg
     1561 gagacattgt cgaactgaat ccttttacgg tccctgttgg cggaaatgct ctggcagttg
     1621 taaatctcga ggcccgcata ccgttgacca aggctttgca ggtggttccg ttttacgacg
     1681 gcggtaacgt cttccggcgg gtaggcgact tatttggcaa gaaagattct acgccggtac
     1741 ccgcgggcga tgttgtggct gcgattaatg ccgcgaacct gcgcgcgcat tggagcaaca
     1801 cagttggtct gggcttccgc attcaaaccc cggtgggggg cgcgctcgcc gtcgactatg
     1861 gtttcctcct aaacccgccg gagtttctga tcccacagcg cggccctggc ggaggatttg
     1921 acggaacgcc ggcgatattt cgcctcaatc gttctcaggt tcatttccgg tttacgcaga
     1981 cgttttagag aggggaaaag ggggaactgg ggagaagggg agaaacggag acggggagac
     2041 acggagaagg ctggcaggca gcggtatcga aaaatgatta attcggattc taacgtcagt
     2101 ataaagtcgc gggtgatagc gagagtcttg tcactcatta ttatttcact tttcgcctta
     2161 actcccttcc caattttcgc ggcttcaaac atcaacgtct tctccccgtc tccctttctc
     2221 cgcgtcaccg cgtccccagc tcaggaagta gtcgatcgca tggtcgcgac cgtcaatgcg
     2281 ggagtacgca ctgatttaat tacttactcg gacctggtgt ggcaactagc tttgcaacca
     2341 aatacgccgc tggtgtcgcc caattcccag gacctcaacc gtgcgttacg gctcgttatt
     2401 gatcagaggt taattcttca ggaagccgaa aagttaccaa cgattgctcc gacggctgaa
     2461 gaagtgaaga acgctcgcga tggactcagc aaagcgtttg cttcggcagc agaatttcaa
     2521 cagcgcttga tgcttgtagg tctgacgtca gaaaaactcg atgagatcct ggaacagcgg
     2581 gtgcgaattg agaagtatct cgactttcgc ttccgcaact tcgtcctgat ctcgcaaaag
     2641 gagatcactg attattacaa ggacgtctat gctccgcgcg tgcggggacg cgggcagatt
     2701 gtgccgacgc ttgaagaggc gcgcgcggag atcgagggca tcctgactga ggctaagatt
     2761 gagtcagaca cggatgcatt ccttgacagt gctcgcgaaa gagcagagat agtgatgttg
     2821 agcggcgtat agtaagttca aagattaaag tccaaagtcc aaagtccgaa gttcaaggtt
     2881 caaagttcaa ggttcaaagt ttcaaaggcc aaaggccttg aatcgatggt gcgcactaag
     2941 aaaactgcga cagttggttt agctctttcc ggaggtgcgg ctcgcgggat cgcccatgtc
     3001 ggagtgttgc aggctctcgt ggagaatcag atcccaatcg attttatcgc cggaacttcc
     3061 gcgggagctt tggtgggagg cgccgccgcc tcaggcatgc cgttatcgga acttgaaact
     3121 ttaggaagaa atctacgctg gcgcgatatt ggacggatga cgatgtcgcg cagtggtgtg
     3181 cagagtaacg agcggctcga acagtatctg cgcgcacg
//
LOCUS       DASUEX010000016         4102 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_921759, whole genome shotgun sequence.
ACCESSION   DASUEX010000016 DASUEX010000000
VERSION     DASUEX010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4102
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_921759"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..964
                     /locus_tag="VFV61_00250"
     CDS             <1..964
                     /locus_tag="VFV61_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4898926.1"
                     /translation="YASNLGWDPRDQINSYDDLAKLGPFQDEWLRGGPVRRWVPKGYA
                     GRPVYTFETGGSTGVPKSRINIEDFRLDYEAFSPTLPDEYFPKGSDWLSLGPTGPRRL
                     RLAIEHLAQFRGGICFMIDLDPRWVIKLLKMGQPKMAEQYKQHVIEQGLTLLRAHDNI
                     RCLFTTPKLLEALCEKVSLKKLGITGVFCGGTEMTPQFHRFAVEEFLEDAYFAPTYGN
                     TLMGLAVHKPRVPEDNFAVIYYPPTPRAMIEVVDPDNPDRIVEYGETGRVRLTTLTKE
                     FFMPRFLERDEAEREPPCELYPWDGVRNVRPFSRFQSTVVEGVY"
     gene            1140..2558
                     /locus_tag="VFV61_00255"
     CDS             1140..2558
                     /locus_tag="VFV61_00255"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX4898927.1"
                     /translation="MLHIPILRKGQPYYSLDLARVPHHRTREPFVEISQANSGLIRRD
                     LRDQQTAREKLTALATAELVDICARAADHFSNDTLPLGEVAQTPEDYVSQVSATTGMP
                     YVLARNNMNKIRGVLAGVGNVLNGLTRNIDWQVLDRGFGELNGQALSFYPRAESLGVV
                     LPNNSPGVHSLWIPAVPLKIPLVLKPGSAEPWTPYRIIQALIKAGAPREAFSFYPTDH
                     AGAGEILRNCGRGMVFGDASSTSLWQGDSRIEIHGPGFSKIVIGEDCIDDWEQYLDVI
                     VRSIADNGGRSCINASGVWVPRHAEKISAALAGRLSQITPRPADDPQAGLAPFVDANV
                     AARISQIIDQGLLEPGAREVTNRNSKRLVSHDGCFYLLPTIVLCESPDHPLANKEFMF
                     PFASVVTAAPETIPEVLGPSLVVTAITTDPKLITKLVNSPQVDRLNVGAVPTSVVSFD
                     QPHEGNLFEHLYGRRAFQRAIA"
     gene            2660..3946
                     /locus_tag="VFV61_00260"
     CDS             2660..3946
                     /locus_tag="VFV61_00260"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX4898928.1"
                     /translation="MKILYLTGGAGQMYCGSCLRDNALAAELIARGHDVTLLPIYTPT
                     LTDEANVSNGKVFFGGISVYLEQYVPLFRRSPRWLDRLWDSKPLLQLASRRSISTSPK
                     MLGELTVSMLRGEDGFQQKEVTKLLEWIKHEAPPDVVSLPYSLLLGLAQPIKEALGRP
                     VCCTLQGEDLFLDGLQEPYRSQAMGLIREHIKHVDAFISVSDYYAEFMPGYLNVPREK
                     IRVVPLGINLEGYHSRPTERSQSFTVGFFARVAPEKGLHVLAEAYRMLRASGELPASR
                     LEAAGYIAPEHKKYLADIEDKMNEAGLGAEFSYHGVLDRDQKIAFLRSLDVLSVPATY
                     DEPKGMFLLEAMACGVPVVQPRRGAFTEIVERTGGGILVEKDDPVSLREGLLRIFRDP
                     ELGTRLGQTGLEGVHQHYSVARMADRALEVYEEAIK"
BASE COUNT          896 a         1120 c         1155 g          931 t
ORIGIN      
        1 ctacgccagc aatcttggat gggatccccg cgaccaaatc aactcttacg atgacctggc
       61 gaaacttggc ccatttcagg acgagtggct gcgcggcggg ccagtgcgac gttgggtgcc
      121 gaaaggttat gccggccggc cggtctatac ttttgaaacc ggtggcagca cgggagtgcc
      181 caagtcgcgc atcaacatcg aggactttcg cctcgattac gaagccttta gccccacctt
      241 gccggatgaa tattttccaa aaggctccga ttggttgtcg ctggggccaa cggggccgcg
      301 gcgtttgcgc ttggcaatcg aacacctcgc ccagtttcgc ggcggcatct gtttcatgat
      361 cgatctcgat ccgcgctggg tgatcaaact gctgaagatg gggcagccaa aaatggccga
      421 gcaatacaaa cagcatgtaa tcgaacaggg actgaccctg ctgcgcgcgc acgacaacat
      481 tcgctgcctc tttacaacgc ccaaactgct ggaagcgctg tgtgaaaagg tctcgctgaa
      541 gaagctcgga atcactggcg tcttctgtgg cggcacggaa atgacgccgc agttccatcg
      601 gtttgctgtc gaagagttct tggaagatgc ctacttcgcg ccgacctatg ggaatacgct
      661 gatgggcctg gcagtgcaca agccgcgggt gcccgaggac aactttgcag tcatctatta
      721 tccaccaaca ccgcgcgcga tgattgaggt tgtcgatcct gacaatccgg atcgcatcgt
      781 cgaatacggg gagactgggc gagtgcgttt gaccacgctg acgaaagagt ttttcatgcc
      841 tcgctttctc gaacgcgatg aagccgaacg cgagccgcct tgcgagcttt atccctggga
      901 tggtgtgcgc aacgtacgcc cattttcgcg atttcaatca actgtcgttg aaggagtcta
      961 ctgattgcca attgccgaat gccgattgcc aattggtttt tggatctttg gttttaggtc
     1021 tttggttttc ttctaagcgt agggaatgac caggcattaa agaacagaat caaggtcaaa
     1081 agccaaaggc caaagaccaa aacaaatcgg caatcgccat tcggcaatcg gaaattgata
     1141 tgttgcacat tcccattctt cgcaaaggcc aaccatatta cagcctcgat cttgctcgtg
     1201 ttccgcacca tcgaacccgc gaaccgtttg tcgaaattag ccaggctaac agcggactga
     1261 ttcgacgcga tctgcgcgat cagcagacag caagagagaa actcactgcg cttgcaaccg
     1321 ccgagttagt cgacatctgc gcacgcgctg ccgatcattt cagcaacgac accttgcccc
     1381 tgggcgaggt tgcgcagact ccggaagact acgtgagtca ggtttcggcg accaccggca
     1441 tgccctatgt tctcgcccgc aacaacatga acaagattcg tggcgtgctc gccggagttg
     1501 gaaacgtgct taacggcttg acccgcaaca tcgattggca ggttttggat cgaggtttcg
     1561 gcgaactcaa cggacaggcc ctcagttttt atccgcgagc ggaatccctc ggtgtggttc
     1621 tgcccaacaa ctcaccgggc gtgcactcac tctggattcc cgcagttccg ctgaagattc
     1681 cccttgtact caaacctggc agcgctgaac cgtggactcc ttaccgcatc attcaggcct
     1741 tgataaaggc gggcgctccc cgggaggcat ttagcttcta tcccaccgac catgccggcg
     1801 ctggggagat cctgagaaac tgcggacgcg ggatggtttt cggagacgct tcatccacca
     1861 gcctctggca gggcgactcg cgaatcgaga ttcacgggcc cggcttcagc aagatcgtca
     1921 tcggtgaaga ttgtatcgac gactgggaac agtatctcga cgtgatagtg cgctcgattg
     1981 ctgataacgg cgggcgctcc tgcatcaatg cttcaggggt ttgggtaccg cgtcacgctg
     2041 agaaaatcag tgcagctctc gctgggcggc tatcccaaat cacgcctcga cccgcggacg
     2101 acccgcaggc aggactggct ccattcgtag acgccaatgt cgccgcgcgc atttcgcaaa
     2161 ttattgacca ggggctgctg gaacccggag cacgcgaagt aacgaatcga aacagcaaac
     2221 gcttggtcag tcacgacgga tgcttttact tattacccac gatagtgctc tgcgagagtc
     2281 ccgatcatcc gcttgcgaat aaggagttca tgtttccttt cgcaagtgtg gttaccgctg
     2341 cgccagaaac gattcctgaa gtgttaggcc caagcctggt agttaccgcg atcactaccg
     2401 atcccaaatt aataaccaaa ctggtgaact ctccccaggt cgatcgcttg aacgttggtg
     2461 ctgtgcccac cagcgttgtg agttttgacc aaccacacga aggtaatctc ttcgaacatc
     2521 tgtacggacg gcgtgccttt cagcgcgcga ttgcttgagt cggagcgacc caattcctgt
     2581 agaggcatcc ctccggcggc gcctgcagac tcgcacggaa aggcgcgccc acagagggac
     2641 gcccttacaa cttagttaag tgaaaattct ctatctcaca ggcggcgcgg gacagatgta
     2701 ttgcgggagt tgtctgcgag acaacgccct ggccgccgaa ctgatcgctc gcggtcacga
     2761 cgtcacctta cttccaatct acacgccgac actgactgat gaagcgaatg taagtaacgg
     2821 caaagtgttc tttggtggca tcagtgttta ccttgaacag tacgttccgc tctttcgcag
     2881 atcgccgcgt tggctcgatc gcttgtggga ttccaagccg ctgttgcaac tggcttcgcg
     2941 ccgttccatt tccacgagcc cgaagatgtt gggcgaactt accgtttcca tgttgagagg
     3001 cgaggacggg tttcagcaaa aggaagtgac taaactgttg gaatggatta agcacgaagc
     3061 gcctcccgat gttgtgagtt tgccctactc gctcctgctg ggcctggcac agccgattaa
     3121 ggaagccctg ggccgaccag tctgttgcac actgcaaggc gaagacttgt ttttggacgg
     3181 cttgcaggaa ccttatagaa gccaggcgat gggtttgatt cgggagcaca tcaaacacgt
     3241 ggatgccttc atttctgtga gtgattatta cgccgagttc atgcccggct atttaaacgt
     3301 gccgcgagaa aagattcgcg ttgtaccgct ggggattaac ctcgagggtt atcattcgcg
     3361 gccaactgag agatcgcaga gtttcacagt tggtttcttc gcgcgcgtcg ctcccgaaaa
     3421 aggacttcat gttctcgccg aggcgtaccg catgttgcgc gcgagcggcg aactccctgc
     3481 ttctcggctg gaggctgccg gttacattgc ccccgagcat aagaagtacc tggcggatat
     3541 tgaagacaaa atgaatgagg ctggtctagg tgctgagttt tcttaccacg gcgtcctcga
     3601 tcgggaccag aagatcgcgt ttctcagaag tctcgacgtg ctctctgtgc cggcgacgta
     3661 tgatgaaccc aaggggatgt tcctgctgga agcaatggct tgcggtgttc ccgtggtgca
     3721 accgcgccga ggtgcattca ccgagattgt ggagcgtacc ggaggtggca tccttgttga
     3781 aaaagatgac ccggtcagcc tgcgtgaagg cctgctgcgt atcttcagag atccagagtt
     3841 aggtacgcgc ctgggccaga ctggcctcga aggggtccac caacattaca gcgtggcgcg
     3901 catggctgat cgtgcgctcg aggtttatga ggaagccata aaatgaacgt gaagctggag
     3961 cgcgagttta gtttcggctt tgccgccgga tgtgtggcgg tggcttcgcc ccgctccgag
     4021 cggatagatg attgtctggg gctacgcccc agatttgaag ttttttgcgc tttcattttc
     4081 gagacaaacg tctgacttgg gg
//
LOCUS       DASUEX010000017         4101 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_921825, whole genome shotgun sequence.
ACCESSION   DASUEX010000017 DASUEX010000000
VERSION     DASUEX010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4101
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_921825"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="VFV61_00265"
     CDS             complement(<1..236)
                     /locus_tag="VFV61_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4898929.1"
                     /translation="MRSLSDQKLDRLRRIATQPDFSGTKYRVIRELGHGGMGTVYLAE
                     DSDLNREVAVKVLNAGADEATLGRRMVREAQIIAR"
     gene            complement(233..982)
                     /locus_tag="VFV61_00270"
     CDS             complement(233..982)
                     /locus_tag="VFV61_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4898930.1"
                     /translation="MDVDTDIGGPLHRFPVTSHSAIIAAASKDDQVRQRALETIVASY
                     WKPAYKYIRIKWQASNEDAKDLTQSFFTTAIEKSYFAGYEAGKASFQTFFRTCLDRFV
                     ANQKKSDQRLKRGAGQDHLSLDFAGVENELTSNLPAPDLNPEDFFHREWVRSLFTLSV
                     ETLEEFYRQTGRELHFKIFELYDLEESAADKVSYGSLANQFALKTTDVTNYLAAARRD
                     FRKILLQKVRELTGTEAEFQAELRALGIDVK"
     gene            1231..1860
                     /locus_tag="VFV61_00275"
     CDS             1231..1860
                     /locus_tag="VFV61_00275"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX4898931.1"
                     /translation="MTSLTFHPAGASRWSDLEDLFGERGACGGCWCMFWRLPRKKFEA
                     GKGAGNKRALKRIVTSGPEPGIIGYLNSEPVAWCAVAPREDYIALERSRILKPVDDQP
                     VWSVSCLFIKKPYRRQGISAQTLRAAVAFAASHGAAIVEGYPVEPSTNTIPDPFLWHG
                     IASAFLAAGFKEVARRSTSRPIMRCEVDGSAHAPKRRRQQNKPSSTANI"
     gene            complement(2249..>4101)
                     /locus_tag="VFV61_00280"
     CDS             complement(2249..>4101)
                     /locus_tag="VFV61_00280"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX4898932.1"
                     /translation="RLRLGTTQNCERDGVAAGIARIDRQAIGLMLMQRIFAISILIVA
                     ALGLNANAQEPPVPAASPQVERGLTVPPIAPDFHPEQKPLPELGRVGVELDRQRPIAL
                     REALALALENNKDIELARDNVKIAEFDLLGSRGAYDPRFSSTSFFERIESPISSFLSG
                     GANGSVVQNDYTASARFEGLAPKFGGNYRLEFSSIRLTSNNQFAALNPQYPTALTLTY
                     AQPLVRGLKFDNNRRLIEIAKKNLSLSDAQFRQRAIETITSVQRAYWDLVFGLRNLQI
                     QRDAVRDARAQLEHNKRLVVEGALAPIDVVAAEAQVAGFEQSLFTALEEVSRSENNLK
                     NLIAVDRQAEIWSVSLIPTDDVELAPPPAVSLEEALRAALENRPELQQSKLIGEVNQL
                     DQKYYREQTKPQIDLVGSYGVVGLAGSISGGLNPFTSSSQELRDRVNLLSLNSGLPRL
                     TDPPAQTLAPELLGGYGQSIQNLFGNRFHNYRIGVQINLPLRNRAAEAQLGRSLVEGQ
                     KIATQRQQLEQSIQVDVRNALQVVRSASARLRAASVAREASEQQYASEQRKLDAGQST
                     VFLVLDRQTALTVARGNELRAQTDLNKAFAELQRATGNALTSNSIVVRVR"
BASE COUNT          908 a         1154 c         1079 g          960 t
ORIGIN      
        1 cgggcgatga tttgtgcttc cctgaccatg cggcggccga gcgtggcctc atctgctccc
       61 gcattcaaca cctttaccgc cacctcgcga ttgagatcac tatcctcggc gagatagacc
      121 gtgcccatgc cgccgtgacc cagttcgcga atcacgcggt acttggtgcc actgaaatcc
      181 ggctgcgtgg caatgcgccg cagtcgatcc agtttctggt ccgaaagaga tctcatttca
      241 catcgatgcc gagtgcccgc aactcagctt gaaactccgc ttcggttccc gttaattcgc
      301 gtaccttctg cagcagaatc tttctgaagt cgcgccgagc tgcggctaga tagttagtaa
      361 catccgtggt cttcaaggca aactgatttg ccagcgagcc atatgagact ttatcggcgg
      421 cgctctcctc aagatcgtaa agctcgaaga tcttgaagtg gagctcgcgt cccgtttgcc
      481 tgtaaaactc ttccagtgtc tcgaccgaga gtgtaaagag actgcgaacc cattcgcggt
      541 ggaagaagtc ttcgggattc aaatcgggtg cgggaagatt gctggtgagc tcattttcaa
      601 cgccggcaaa atccagcgat aagtgatcct ggccggctcc gcgcttcagc cgctgatccg
      661 acttcttctg gttggctaca aaacggtcca gacatgtgcg aaagaaagtc tgaaaactgg
      721 ctttccccgc ctcgtaacca gcgaaataac ttttttcaat cgccgtcgta aagaaactct
      781 gcgtcaggtc cttcgcgtct tcattcgacg cctgccactt gatacggatg tacttgtaag
      841 ctggtttcca gtaactggcg acgatggttt cgagagcgcg ttgccgcacc tgatcgtctt
      901 tgctggcagc ggcaatgatt gctgagtggc tggtgacggg aaaacgatga agcggtccgc
      961 cgatgtcagt atcaacgtcc atggaatgat tgggtcattg aatcattgaa tcattgaatc
     1021 attgagtcat tgagtcattg agtcattgaa ccattggttt attgaatcat caaatcagtg
     1081 aatcactgaa atcctggatt gcgctgaatg gattccccgg ggttcggacg atacaatgac
     1141 tcaataactc gatgattcaa tgattcaatg attcaatgat tcaatgaccc aatgacccaa
     1201 tgacccaatg actcaatgat tcaatctaaa atgactagtc tcactttcca tcccgccggg
     1261 gcttcccgct ggtctgattt agaggactta tttggtgaac gcggcgcctg tggcggttgc
     1321 tggtgcatgt tctggcgctt gccgcgcaag aagtttgaag cgggcaaggg agcaggaaac
     1381 aagcgggcgc tgaaacgaat cgtgacttcc ggacctgagc cgggcatcat cggttacctg
     1441 aattccgaac cggtagcatg gtgcgcagtt gctccgcgtg aagattacat cgcgcttgag
     1501 cgatcgcgca ttctaaaacc tgtggatgac cagccggtat ggtctgtttc gtgtttgttc
     1561 atcaagaagc cctatcggcg tcaggggatc tccgcgcaga ctctgcgggc agcagtggcg
     1621 tttgccgcct cgcacggcgc tgctattgtt gaaggttatc cggtggaacc ttcgactaac
     1681 actattcccg atccttttct ctggcacggc atagcttccg cctttcttgc tgccggattt
     1741 aaggaggttg cacgtcgatc gacttcgcgt cccatcatgc gttgtgaagt tgatggttcc
     1801 gctcacgccc ccaagcgcag gcgacaacaa aacaaaccat catcaactgc gaacatctaa
     1861 tatccgattg catctggaca actgtagaga tgccctcaca agagtcatcg caaagacaag
     1921 ttcgtcttgt ggtaaagtag tcacgtttcc cccgagcggt ctcagcccag aacgttacgg
     1981 agattctaga tgaagtgtgg acactgcggc cggcgcacgt cctggatgag ggcaagatgt
     2041 ccaacctgca aggcgaagct cgtccagtgg tacatcatcg ccgtagtgct tttcatcctc
     2101 gcctgctacg ccagcctcat gttgcttgat actttcttaa cgcgcgcctg actcataact
     2161 aaagcgggcc ttaaaacgaa ggcggcgaga ctcgccccaa ggtctcgccg ccttctcgcg
     2221 tgtggtgctg caaaatcggg aagactaatt agcgcactcg cacgacgatg ctgttacttg
     2281 tcaaagcatt ccctgtcgct cgttgcaatt ctgcaaacgc cttgttcaga tcggtctgcg
     2341 cccgcagttc gttaccacgc gctaccgtca aggcagtttg ccgatcaagc accagaaaca
     2401 ctgtcgactg ccccgcatcc agtttccttt gctcactggc atattgctgt tcgctggcct
     2461 cccgagccac actcgcggcc cgcaggcgcg cggaggcgct acgtactacc tgcaaggcgt
     2521 tacgcacatc aacttgaata gattgctcga gctgctgacg ctgcgtggcg atcttctgcc
     2581 cctccaccag cgagcggccc aattgagctt cagccgcgcg attgcgcaac ggcaaattga
     2641 tctgcacccc gatccggtag ttatgaaagc gatttccaaa caaattctga atagactgac
     2701 cataacctcc cagcagctca ggagcgagag tctgagccgg cggatcagtc aaacgtggca
     2761 ggccgctgtt aagagaaagt agattgacac gatcgcgcag ctcttgtgac gaagaggtaa
     2821 agggatttag gccgccactg attgaacctg ctaagcccac gaccccatag ctgcctacca
     2881 gatcgatttg cggcttggtt tgctcgcgat agtatttctg atcgagttgg ttaacttcgc
     2941 cgatcaattt tgactgctgc aactcggggc gattttccag cgccgcgcgc agtgcttcct
     3001 ccagcgaaac cgccggtggc ggcgccagct cgacgtcgtc agttggaatc agtgaaacac
     3061 tccaaatctc cgcttgccga tccaccgcga tcaggttctt gagattgttt tcactgcgcg
     3121 agacttcctc gagcgcagtg aacaagctct gttcgaaccc tgctacctgc gcttcggcag
     3181 ccaccacatc gatcggcgcc agcgcaccct caacaaccaa acgcttgttg tgctccagtt
     3241 gggcgcgcgc atcgcgcact gcgtcgcgct gaatctgtaa gttacgcaga ccaaagacca
     3301 gatcccagta cgcgcgctgc acgctcgtaa ttgtttcgat cgcacgttgc cgaaactgcg
     3361 catccgaaag tgaaaggttc tttttggcaa tctcgatcag gcgacgattg ttatcgaact
     3421 tcaatccgcg cacgagtggc tgcgcatagg ttagggtcag cgccgtcgga tactgcgggt
     3481 taagcgcggc gaactggtta ttactcgtta accggatcga agaaaactcc agccggtagt
     3541 tgccgccgaa tttcggtgcc agtccctcaa agcgagcgct tgcagtgtag tcgttctgca
     3601 ctaccgagcc gtttgcacca ccgcttaaga aactcgagat cggactttcg atgcgctcga
     3661 agaaagaagt ggaactgaag cgtggatcat aagcgccacg cgatcccagc agatcaaatt
     3721 cggcaatctt cacgttgtcg cgggctagct caatatcttt gttgttctcg agggcaagcg
     3781 ccagcgcctc gcgcaaagcg atggggcgtt gccgatcaag ctctacaccc acgcgtccga
     3841 gttccggtaa aggcttctgt tctggatgaa agtccggagc aattggcgga actgttaagc
     3901 ctcgctcaac ctggggtgaa gcggccggaa caggcggctc ctgagcgttc gcgttcaggc
     3961 ccagcgcggc cactatcaag atcgaaattg cgaagatacg ttgcattaac attagtccta
     4021 ttgcttgcct atcaatccga gcgatcccag cagcgacgcc gtcgcgctcg cagttttgcg
     4081 tcgtacccag gcgaaggcgc c
//
LOCUS       DASUEX010000018         2026 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1264345, whole genome shotgun sequence.
ACCESSION   DASUEX010000018 DASUEX010000000
VERSION     DASUEX010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1264345"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="VFV61_00285"
     CDS             complement(<1..300)
                     /locus_tag="VFV61_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4898933.1"
                     /translation="MNVRTASDLMGAIAEHASAELFDKEIKDFSIDSRSVDAGELFFA
                     LSQEDYARAGFNGTFADGHQFIAEAFARGAVAAVARKNRVRGDAKLEALRERLLLV"
     gene            complement(297..536)
                     /locus_tag="VFV61_00290"
     CDS             complement(297..536)
                     /locus_tag="VFV61_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4898934.1"
                     /translation="MIPQRVLKDSYANAVRYRVEACRKRLREVRANQRAQGIGRIDED
                     ATAAERVVAGPPPSQDFIERSFALEVVELQEAEIP"
     gene            complement(616..>2026)
                     /locus_tag="VFV61_00295"
     CDS             complement(616..>2026)
                     /locus_tag="VFV61_00295"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEX4898935.1"
                     /translation="SPGQTVVLTIDQSVQYRAEQALHNAVQKSRAKSGTAVVLDPRTG
                     EILALANMPTFDPNNVRESSSVQRSNWALQNVYEPGSTFKIVAYSAAIEKGLVKPDDR
                     IDCQMGSITVAGRLVHDHHAFGTLTISEALAKSSNVAAIKLGIRVGDQSMYDYMTRFG
                     FGSRTGVELPGETAGLLRPVARWQPSSIGSIAIGQEVAVTPLQMAGAFGALANNGVRV
                     APHLVREIRTSSGVTVYRSAPEQRRVVNESTAVALRGMLEGVTLNGTAKQAQLDGYTA
                     AGKTGTAQKIDPKTRSYSKTKHVASFVGFAPVGNPAVVIVVVIDEPAGSYHGGDVAAP
                     VFREIAEQILPDLGVAPDTDFKTPMELIAEARPNPASGEGTDDTRRVVQEPDESYPRI
                     AERDPKGREIVYAAASTRAMLMPNLRGRSLRDVAQACTQLGLQLEAHGEGRVLRQTPS
                     PGMEVQPGQIVYVEFARSN"
BASE COUNT          412 a          629 c          527 g          458 t
ORIGIN      
        1 aaccagcagc aagcgctctc gtagagcttc gagcttcgca tcgcccctca cccggttttt
       61 gcgtgccacc gcagccactg cgccccgcgc aaaagcttcg gcaataaact ggtggccgtc
      121 ggcgaaagtg ccattaaaac cggcgcgggc gtagtcctct tgcgataagg caaagaacag
      181 ctcgcccgcg tcaactgacc gggaatcaat ggaaaagtct tttatttctt tgtcaaacaa
      241 ctcggcgctt gcatgttcgg caatggcgcc catcaaatcc gatgcggttc taacattcac
      301 gggatttccg cctcttgcag ctcgaccacc tcgagcgcaa acgagcgctc aataaaatct
      361 tgtgacggcg gcgggccggc aacaactctt tctgctgcgg tcgcgtcttc atctatcctt
      421 ccgatgcctt gtgctcgctg gttggcccgc acttcacgca accgcttcct gcaggcttcc
      481 actctgtatc ttacggcatt tgcgtagctg tctttgagca ctcgctgggg gatcatatcg
      541 ggtgcctttc cctctcgttt tttcaacggg cggctgccgt taagtcggga acggcggatc
      601 gcgcgaatta ggatttcaat tcgatctggc aaattccaca tacacaatct gaccgggttg
      661 gacctccatt cccggcgaag gggtttgacg cagtacacgc ccttctccat gcgcctcgag
      721 ctgcaaaccc agttgcgtgc aagcctgggc cacgtcacgc aggctgcggc cgcgcaaatt
      781 cggcatcagc atcgctctgg tgcttgccgc ggcgtaaaca atctcgcgcc ccttgggatc
      841 tctctcggca atccgtgggt aactctcatc gggctcctgc accactcgac gcgtgtcgtc
      901 tgttccttct cctgaagcag ggttgggtct ggcctctgca atcaactcca tgggcgtctt
      961 gaaatctgtg tctggcgcga ctcccagatc gggaaggatc tgctcggcta tctcgcgaaa
     1021 tacaggagcc gcgacatcgc cgccatgata agagcccgct ggttcgtcaa tgacaactac
     1081 gatcaccacg gctgggttac caacaggcgc aaaacccaca aacgacgcaa cgtgtttggt
     1141 tttagaatac gaccgtgtct ttggatcgat cttttgtgca gtgcccgttt ttcccgcagc
     1201 cgtatagcca tcgagctggg cctgctttgc cgtaccgttg agcgttaccc cttcgagcat
     1261 tccccgaaga gccaccgccg tgctctcatt tacaactcgc cgctgctcag gagctgaacg
     1321 atagacagtg acgccggacg aggtccgaat ctcccgtacc aaatgaggag ccacacggac
     1381 tccattgttt gcgagcgcac cgaaagcacc tgccatctgc agcggtgtaa ccgccacttc
     1441 ctgcccgatg gcaatcgagc caatcgatga aggctgccaa cgtgccaccg gccgcagcaa
     1501 tcctgccgtt tcccccggca actcaactcc cgttctcgag ccaaatccga agcgggtcat
     1561 gtagtcatac atcgactggt cgccgactct gataccaagc ttgatggcag caacgttgct
     1621 ggatttggca agcgcttctg aaatcgtcag tgtgccgaac gcatgatgat cgtggaccag
     1681 gcgtcctgcg accgtgattg aacccatctg gcaatcaatc ctgtcatccg gcttgaccag
     1741 ccctttttca atggcggctg aataggcgac gatcttgaag gtcgacccag gctcgtaaac
     1801 gttttgcaag gcccaatttg atctctgcac actggacgac tcacgaacat tgttcggatc
     1861 aaaggtaggc atattggcga gggccagaat ttccccggtg cgcggatcaa gcactactgc
     1921 cgtacctgac ttcgcgcgcg acttttgcac agcgttatgg agcgcctgtt ccgcgcgata
     1981 ctgcaccgac tggtcgatcg tgagaactac cgtctgaccc gggctc
//
LOCUS       DASUEX010000019         3077 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_807799, whole genome shotgun sequence.
ACCESSION   DASUEX010000019 DASUEX010000000
VERSION     DASUEX010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3077
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_807799"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="VFV61_00300"
     CDS             complement(<1..315)
                     /locus_tag="VFV61_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4898936.1"
                     /translation="MPSVAELLLKSIPELRDIFIDRGRRVPQGLVEALEADSRHGAQQ
                     LARRIRERRRTNRSEGQRLHSLLRFEIELWNDGYSLVAGVDEAGMAPLAGPVVAGAVI
                     LPQ"
     gene            483..1025
                     /locus_tag="VFV61_00305"
     CDS             483..1025
                     /locus_tag="VFV61_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4898937.1"
                     /translation="MAFRLLGFLPLVFFLAQAIHYWRISELGNMLWMCNIGNLVLAIG
                     LFLKRPLLVRVAVIWMVPGLVVWFVYVVLAWGIFLSSTLAHVGGFVVGVVALQRVRMD
                     RVSWLYALGWYFMVQLFSRLFTAAALNVNVAHAVDPGWQQTFNAYWKFWVVLLLVAIA
                     LLGGLNLIFYKLWPEREAEV"
     gene            complement(1219..2046)
                     /locus_tag="VFV61_00310"
     CDS             complement(1219..2046)
                     /locus_tag="VFV61_00310"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEX4898938.1"
                     /translation="MPKKVQRNEEPADEQSGGRPFWSGTLTFGLVSVPVNLFPATRST
                     RTSLRMLGPDGQPLARKYYSQKTEKDLDADEIVRGYEIEKDKFVIVTDEELERLAPEK
                     TRDIDLKLFVPEESIPPIYFERGYFLTPAANSHKAYKLLAETMKRGRQAGVATFVMRG
                     KEYLVAIFSDQGILRAELMRFADELRTPSDLELPKKDKVPAATVRKFEKLISERTKKQ
                     FATSKLTDAQNEKLLKLVKKKKSQRKNIVEVEVEEKTSERKVIDLVAVLKKSLSAKA"
     gene            complement(2039..2824)
                     /locus_tag="VFV61_00315"
     CDS             complement(2039..2824)
                     /locus_tag="VFV61_00315"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEX4898939.1"
                     /translation="MAARAIWKGILKIGSAKVPVKLYSAVSDRTVRFHILDDRSKMRI
                     KQHMINPESGAEVATEEIQKGYELEPGRFVILTQDELKSLEPEPSREIEVGEFIPPTQ
                     ISQQWYERPYYLGPDGDQKAYFALAEALHKRELEGVAHWVMRNQAYVGALRAEGDYLM
                     LVTLRNAAEVISVRELSKPAGPAPTAKELSMAKHLVELMQGKFNPAEFKDEYRERVME
                     FIEKKAKGHAPRLQAVKTKRKTGSLDSVLAKSLAALKKEKRAA"
BASE COUNT          617 a          825 c          798 g          837 t
ORIGIN      
        1 ctgtggcaag atcaccgccc cggcaacgac cggtccggca agcggcgcca ttcccgcttc
       61 gtcaacgcct gccacgaggg aatagccatc gttccaaagt tcaatctcaa aacgtagcaa
      121 cgagtgcagc cgttgtcctt ctgagcgatt agtgcgacga cgctcgcgga tcctccttgc
      181 gagttgctgt gcgccgtggc gggagtcagc ttccagtgct tcaaccagtc cctgaggtac
      241 acgtcttccc cgatcaataa agatgtctct tagttcagga atagatttaa gcagaagctc
      301 ggcaacagac ggcatgatga atctgattcg ttgttgtggt gttcaagatc gtgtcgggcg
      361 aatgctattc taaattgaaa tatgaaattg cgcgagagtc ggtttttgat ttagtgtttt
      421 cgactaattg gcaccaagag agacagatga attcatcccc cgatcagctt aagcaaaacg
      481 atttggcctt taggctcctc ggtttcctgc cgcttgtttt ctttcttgct caagccatcc
      541 attactggcg tatcagcgag ctcggcaaca tgctctggat gtgcaacatc ggcaatctgg
      601 tgttggcaat cggactattt cttaagcgtc ccctccttgt tcgtgttgcc gtgatttgga
      661 tggtgcccgg acttgtggtg tggtttgtct acgtcgtgct ggcctgggga atcttcctgt
      721 cgtcgaccct ggcgcacgtt ggcggattcg ttgtgggggt cgttgctctc cagcgcgtac
      781 gcatggatcg cgtgtcgtgg ctttacgccc tcgggtggta cttcatggtg cagttatttt
      841 cacggctgtt tacggcggct gcactcaatg taaatgtcgc tcacgctgtc gatcctggat
      901 ggcaacaaac ctttaacgcc tactggaagt tctgggtagt gttactgctc gtggcgatcg
      961 cgctattggg aggcttgaat ttgattttct acaaactttg gccagagcga gaggcagagg
     1021 tttaagtact gtcgggaaca actcggaagt gcatatcctt agacgaacct tgagctgtcg
     1081 gcatcacgac tctgatggtt ttgcatgaac aactgttaaa gaagtgcttt aggtaggggc
     1141 ggtttagaaa ttggatgcag tcgcgcttgt ttggctccga ggacagtcgt gttgttcgca
     1201 ggagcgagcc gtcgaaagct aggcttttgc ggatagactc tttttcaaaa cggccaccag
     1261 gtcgatcacc ttacgctcac tggttttttc ctccacctca acttcaacga tgttctttcg
     1321 ctgcgacttt ttcttcttaa ccagtttgag gagtttctcg ttttgcgcgt ccgtgagttt
     1381 tgaggttgca aactgcttct tagttcgctc actgattagc ttctcaaact ttcgcactgt
     1441 cgccgcggga accttgtcct ttttggggag ttcaagatca ctcggggtgc gcaactcatc
     1501 agcgaatctc atcagctctg cacgcaggat cccctggtcg gaaaagatcg ccactaaata
     1561 ttctttgcca cgcatcacaa aagtggcgac gccggcctgt ctgccacgct tcatggtttc
     1621 ggcgagcagc ttgtaagcct tgtgcgagtt ggccgcgggc gtgaggaaat agccgcgctc
     1681 aaaatagatc ggaggaattg attcctcggg cacaaacaac ttcaggtcga tgtccctggt
     1741 cttctccggc gcgagacgct cgagctcttc atcggtcacg attacaaact tgtccttttc
     1801 aatttcgtaa ccgcgaacta tttcgtcggc gtccagatcc ttttcggttt tctgtgagta
     1861 gtatttgcga gcgagcggct ggccatctgg tcccaacatg cgcagtgacg tgcgagtcga
     1921 tcgcgtcgcc ggaaacaaat taaccggaac actaaccaac ccgaacgtga gcgttcctga
     1981 ccagaaaggg cgtccgcctg attgctcgtc agcgggctct tcattccttt gaactttttt
     2041 aggcagcgcg tttctccttc ttcaaagccg caagactctt ggccagcacg ctgtccagag
     2101 atccggtttt gcgttttgtc ttaactgctt gcaagcgcgg cgcgtggcct ttggctttct
     2161 tttcgatgaa ttccataaca cgttcgcgat actcgtcttt aaactctgcg ggattgaact
     2221 tgccctgcat cagttcaacc aaatgcttcg ccatgctcag ctcttttgct gtcggtgctg
     2281 ggcctgccgg tttgctgagc tctcgcacgg aaatcacttc cgcggcattc cgcagggtga
     2341 ccagcatcag ataatcgccc tccgcccgca gcgcgccgac gtaagcctgg ttgcgcatta
     2401 cccaatgcgc cacaccttcg agctcgcgtt tgtgtagggc ttcggccagg gcaaagtagg
     2461 ctttttgatc gccgtcagga ccaaggtagt agggtcgctc gtaccactgc tggcttattt
     2521 gggtgggcgg aataaactct ccgacttcaa tctcacgaga gggttcaggc tcaagactct
     2581 taagctcgtc ttgggttagg atcacaaacc taccgggctc cagttcataa cccttctgaa
     2641 tctcctcggt agccacttcg gcacctgatt caggattgat catgtgctgc ttgatccgca
     2701 tcttgctgcg atcgtcaagg atgtgaaagc gcacggtgcg atcactaacg gcggagtaca
     2761 acttcaccgg cactttcgcc gagccgatct ttaatatccc tttccaaatg gcgcgggcag
     2821 ccatcacacc acctcttcct ttttccgcgc ttgaagacag cccgggcaaa cgaattcaaa
     2881 cgttgtcgtt ctttctatgc acactggaca aatcgctgcg tcgcaatcga agcaatagac
     2941 ttcggtgcga tgagcgtagt ggtggctgca actggagcaa ccttcactat cttccgcgag
     3001 ccaccacggc tgcaggcgtt cggaatttgc cttagccctt ctcatgaatt agcacccctg
     3061 gacatttctt tgcttga
//
LOCUS       DASUEX010000020         3195 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1148644, whole genome shotgun sequence.
ACCESSION   DASUEX010000020 DASUEX010000000
VERSION     DASUEX010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3195
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1148644"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            115..873
                     /gene="larB"
                     /locus_tag="VFV61_00320"
     CDS             115..873
                     /gene="larB"
                     /locus_tag="VFV61_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018281842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HEX4898940.1"
                     /translation="MDARELERLLRSFKNEELSAEDAAQQIKNLHFEDIGFARVDHGR
                     STRQGFPEVVFGSGKTRAQVVSIVEKLAARSPNVLVTHTDAETFGEVRNVIAAAEWHE
                     SARLIRIQRDSTELGVGTIAVVTAGTSDIPVAEEAALTAEAMGNRVDRVWDVGVAGIH
                     RVLAERELLQRARVIIVAAGMEGALPSVVGGLVSVPVVAIPTSIGYGASFGGVAALLG
                     MLNSCASNVTLVNIDNGFGAGFVASLINRKWIGD"
     gene            1012..1656
                     /locus_tag="VFV61_00325"
     CDS             1012..1656
                     /locus_tag="VFV61_00325"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: protein motif:HMM:TIGR00421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0106141 - flavin prenyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HEX4898941.1"
                     /translation="MEFTVAITGASGSIYAQRTLVQLAASGLVERINLIMSGAAETVA
                     QVELGVDLRAASGEKINSWIGLPRDSKLIRLHRLDNMAARPSSGSHPQEGMVVVPCSM
                     GTLGAIASGAGTNLIHRAADVTLKESRKLILVPRETPYNAIHLENMLRLARAGAHIIP
                     ASPGFYHRPATIEALVDHLVFRILDHLGVPHSQATRWQGKTEKASDLATINEAT"
     gene            1667..2542
                     /locus_tag="VFV61_00330"
     CDS             1667..2542
                     /locus_tag="VFV61_00330"
                     /inference="COORDINATES: protein motif:HMM:TIGR01475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HEX4898942.1"
                     /translation="MPPLLHNVRTTLEMIKIEHTLFALPFALLGAVLAASGLPTVRQL
                     FWIAVAMVGARSTAMAFNRIVDREYDARNPRTRSRAIPSGALSVSFVWMFTIVAAALF
                     LLAAAMLNRLTLILAPVALASVLLYSFTKRWSLLSHFVLGWCLAIAPTGAWVAVRGSL
                     DNPIPLLLSLVVLLWTAGFDVLYACQDYDFDTVAGLHSIPRSFGIAGSLWIARGLHAA
                     AFAALIALYLATRLGSVAFVGVIATGILLIYQHRLVKADDLSRLNAAFFTTNAFVSVI
                     LCLTFGAAVIIRMYS"
     gene            2539..>3195
                     /locus_tag="VFV61_00335"
     CDS             2539..>3195
                     /locus_tag="VFV61_00335"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX4898943.1"
                     /translation="MNFSDDSRLLEIASKVEEGERLSLADGLDLYATNDLLALGKLAD
                     KVRRRKHGLTTYFNVNRHFNHTNVCYADCKFCGFYRTPRQADAYTHNIEESLRIAGEA
                     VAEGATELHIVGGLNTKLPFSYYTDLLSSLKRQYPQLHLKAFTMVELDHFAHFYKMSD
                     DDVIQKLIEAGMDSCPGGGAEIFREPTRSMICAHKCDADRWLELSGKVHASGLKTNAT
                     M"
BASE COUNT          699 a          872 c          861 g          763 t
ORIGIN      
        1 aatgaactaa tgacctctga ccactaacaa ctaacaacta acaactaaca actgacaact
       61 gacaactaac aactgactac tgacctactg actactggct actaactact gataatggac
      121 gcacgtgaac tcgaacggct actgcgcagc ttcaaaaacg aggagctctc ggcggaagac
      181 gcggcgcagc agattaagaa cctccacttc gaagatattg gcttcgcgcg cgtcgaccac
      241 ggccgctcga cgcggcaggg ctttcctgaa gttgtatttg gatccgggaa gacacgagca
      301 caagttgtca gcatcgtgga aaaattggcc gcgcgttctc ccaacgtcct cgtcacacac
      361 acagacgcgg aaaccttcgg tgaagttcgc aatgtgatcg cggccgcgga atggcatgaa
      421 tcagcgcggt taattcgcat tcaaagggac tcgacagagc tgggagtcgg cacaatcgcg
      481 gtggtcacag cgggaaccag tgatattcca gtagccgagg aagctgcgtt aacggcggaa
      541 gcgatgggca atcgagtgga tcgcgtttgg gatgttggcg tcgctggaat tcaccgggtc
      601 ctggccgaac gcgaattgct acaaagagcc cgtgtgataa tcgtggcggc aggaatggaa
      661 ggggcgctgc cctcagtagt gggcggactc gtcagcgttc cggtagttgc aattccgacc
      721 agcatcggct acggagcgtc gtttggtggc gttgcggccc tcctgggaat gctcaattcc
      781 tgtgcctcca atgtgacgct cgtcaacatc gataacggat tcggagccgg ctttgtcgcc
      841 agcttgataa accgaaaatg gattggcgat tgacgattgc ggattgccga ttggcggata
      901 accattcgtc ttcggtactt ttgaaattgg caattggcaa ttgacaatcg tcaatctgca
      961 cgctcgcttg ccgcgtgacc atcccgccaa gtacacttcc actaccgctt catggaattt
     1021 actgttgcaa ttacgggcgc ctcaggctca atttacgcgc aaagaaccct cgtccagctt
     1081 gccgccagcg gcctggtgga acggattaac ctaatcatgt ctggtgcggc cgaaactgta
     1141 gctcaggtcg agcttggcgt tgatttgcgt gccgccagtg gcgagaagat taattcgtgg
     1201 attggacttc ccagggattc gaaactaatt cgcctgcaca ggttggacaa catggccgcg
     1261 cgcccgtcct caggttcaca cccgcaggaa ggcatggttg tcgttccctg ttcaatgggt
     1321 acgctcggag caatcgcttc cggcgccggc actaacctga tccatcgggc cgctgatgta
     1381 acgctgaaag aaagtcgtaa gttaatactc gttccgcgcg agacccctta caacgcgatt
     1441 catctggaaa acatgctgcg actggcacgc gctggcgccc atatcattcc ggcgagcccc
     1501 ggcttctatc atcgacccgc tactatcgaa gccctggtgg atcatcttgt atttcgcatt
     1561 cttgaccacc tcggcgttcc gcactcgcaa gcgacccggt ggcaaggtaa gactgaaaaa
     1621 gcgagcgatc tcgcaacgat caatgaggcc acctaagact cattcagtgc cgccactctt
     1681 acacaatgtt cgcaccacgc tcgaaatgat taagatcgag cacaccctct tcgccttgcc
     1741 ttttgcgctg ttgggcgccg tgctggccgc cagcggactg cccaccgtgc gacaactgtt
     1801 ctggatcgca gtggcgatgg tcggtgctcg ctcgacggcg atggcgttca atcgaatcgt
     1861 cgatcgggaa tacgacgcgc gcaacccgcg cactcgttca agggcaattc cctcgggcgc
     1921 gctttcagtc agtttcgtct ggatgtttac gatagtggca gccgcactct ttcttctcgc
     1981 cgccgcgatg cttaatcgcc tgacgttgat tctcgcaccg gtggccctgg cgagtgtgtt
     2041 gctctattcg ttcactaagc gctggtcgct gttgtcgcac tttgtgctcg gctggtgcct
     2101 ggcaatcgct ccaacaggcg cgtgggtagc cgtacgcggg tcactcgata acccgattcc
     2161 gcttctgctc tcgctcgttg tcctactttg gactgctggc tttgatgtgc tttacgcatg
     2221 tcaggattac gactttgata ctgttgcagg attgcattcg attccccgta gctttggcat
     2281 cgcgggttca ctctggatcg ctcgcggctt gcacgcagca gcgttcgccg cgttgattgc
     2341 gttatacttg gcaacgagat taggcagcgt agcattcgta ggcgtgatcg ctactggaat
     2401 actcttgatc taccagcatc gtttagtgaa ggcagacgat ctcagccggc ttaacgcggc
     2461 gttttttacc actaacgcct ttgtgagcgt cattctctgt ttgacgtttg gcgccgcagt
     2521 aatcataagg atgtattcat gaacttttca gacgattccc gattactcga gattgcgagc
     2581 aaggttgaag agggagaacg gctctcactt gctgacggtc ttgatctcta tgcaaccaat
     2641 gacctcctgg ccctcggtaa gctggcagac aaagtgcggc gccggaaaca cggattaact
     2701 acctatttca acgttaaccg tcatttcaat catacgaatg tttgttacgc agactgcaag
     2761 ttttgtggct tttaccgcac gcccaggcaa gctgacgcct atacgcacaa cattgaagag
     2821 tcgctgcgga tagccggtga agcggttgcc gaaggtgcaa cggaactaca catcgttggt
     2881 ggcttgaata cgaaattgcc gtttagttac tacaccgatt tgttgtcgtc cctgaagcgc
     2941 cagtacccgc aacttcattt gaaggccttc accatggtgg agctcgatca ttttgctcac
     3001 ttctacaaaa tgagtgatga cgatgtgatt caaaagctca tcgaagccgg catggattcg
     3061 tgtcctggcg gtggcgcgga gatttttcgc gaaccgactc gttcaatgat ttgcgcccat
     3121 aaatgcgatg cagatcgctg gcttgaactc tcaggaaagg tgcatgcgtc cggtttgaag
     3181 acaaacgcca ccatg
//
LOCUS       DASUEX010000021         2194 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1606845, whole genome shotgun sequence.
ACCESSION   DASUEX010000021 DASUEX010000000
VERSION     DASUEX010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1606845"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..381)
                     /locus_tag="VFV61_00340"
     CDS             complement(<1..381)
                     /locus_tag="VFV61_00340"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF028191.4"
                     /note="catalyzes the radical-mediated insertion of two
                     sulfur atoms into an acyl carrier protein (ACP) bound to
                     an octanoyl group to produce a lipoyl group; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEX4898944.1"
                     /translation="MSRELRVISNNPKPRQPKPEWLKIRLGDPTNQNNVLKLIEGLNL
                     HTVCQEARCPNIFECWSDRTATFMLGGDICTRHCGFCAVGKGQPGMLDENEPRNVAEA
                     IRHLDLAHTVITSVNRDDLSDGGAA"
     gene            561..1130
                     /locus_tag="VFV61_00345"
     CDS             561..1130
                     /locus_tag="VFV61_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4898945.1"
                     /translation="MKRALGYLFAASLLLFALNDARADLAPAPSPQKPGKIVLHTSLS
                     VVPDSKAYQARLQISQDSLNEIRAALGEVPGNGSSAQSLAHNSQRTIIAGLSLFLALS
                     FGGVWLARSAARRSQKAMAAVLTGVAVLGAAAVITRANAGPPPAFRWRGLPQNLSEGR
                     ATSGGVDIEIVPEGNGMKLIIPLQKKSGE"
     gene            1134..2177
                     /locus_tag="VFV61_00350"
     CDS             1134..2177
                     /locus_tag="VFV61_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4898946.1"
                     /translation="MERAVDLEISLWRDKRSGFGAFSSPAQAKLFVTIVLAATLLSAL
                     LASWIPLQFSIVTVFLFAGPHNYFEFRYFLTRLPVRFGKSRNFFLLAFAGILFLTLAY
                     LSLPALYYAGLWSGTNWPTMIAAWNTLMLLWVAALVWSRGKQRRGRDWFWAWPIALVL
                     ISLNWMGPELCSLGIVYLHPLIALWFLDRQLRRTRPEWLPAYHRCLALLPFVIVAICW
                     RMNGLATLQDDNGLAWRITQHAGAGLLPNISSNLLVSVHVFLEMLHYLVWLVALPLLT
                     STGAWDLKKIPLVQHPRGFPRLIAAVIVLGLLLVALLWLGFGLDYGATRDLYFAVAIA
                     HVLAEAPFLLRTM"
BASE COUNT          423 a          640 c          581 g          550 t
ORIGIN      
        1 cgccgccccc ccatccgaaa gatcatcgcg attcacagag gtgatgactg tgtgcgcgag
       61 gtccagatgg cggatggctt cagccacatt ccgcggctcg ttctcatcaa gcattcccgg
      121 ctgacccttc ccaacggcgc aaaagccgca atggcgggtg caaatgtctc cgcctaacat
      181 gaatgttgcg gtgcgatcgg accaacattc gaagatgttc ggacaacggg cttcctggca
      241 aacggtatga agattcaggc cctcaatcag ttttagtacg ttgttttggt tcgtcggatc
      301 gcccagccga atcttgagcc attcgggttt tggctggcgc ggttttgggt tattactgat
      361 tactctgagc tcgcgactca cttcttttca cctcgtttga cggcagattt tcattctagc
      421 atccggttag gatttaggca cttggtggcg aaatcgccgg tagaactttg aaacccgaca
      481 agtacacttg cgttatactg cgcggtcgtc cacaccagcc ttgaacggcc atccagccct
      541 ttttccaaag gagatagaaa atgaagagag cgctcggata cctattcgcc gccagcctgt
      601 tgttgttcgc actcaatgat gctcgtgccg acctcgcacc tgcaccctca ccgcagaaac
      661 ccggcaagat tgtccttcat accagcctgt ccgtagtgcc tgactcaaag gcttaccagg
      721 cgcgactgca gatcagccag gacagtctca acgagattcg tgcggccttg ggagaagttc
      781 ccggcaacgg ctcaagtgcg cagagcctgg cccacaactc gcaacgcact atcatcgccg
      841 gcctctcgct ctttctggcg ctctctttcg gtggagtttg gctggcccgc tccgcagccc
      901 gccgcagtca aaaagcgatg gcagcagtgt tgacaggagt ggccgtgctt ggggctgcgg
      961 cggtcatcac acgtgccaat gctggaccac caccggcgtt ccgctggcgc gggctaccac
     1021 aaaatctttc cgaaggacga gcaacatccg gtggagtcga cattgaaatc gtgcctgaag
     1081 gtaatggaat gaagttgatc atcccactcc agaaaaagtc aggcgaataa gcgatggaac
     1141 gcgcagttga tcttgaaatc tctctttgga gagacaagcg gtcaggattt ggagctttca
     1201 gctcgcctgc acaggcgaag ctctttgtca ccatagtcct ggcagccact ctgttatccg
     1261 ccttgttggc gagttggatt ccacttcagt tttcgatagt cacagtcttt cttttcgccg
     1321 gcccgcacaa ctacttcgag tttcgctact tcctcactcg cttgccggtg cgctttggta
     1381 agtcacgcaa cttctttctg ctcgcctttg ccggaatcct gtttctcaca ctcgcgtact
     1441 tgtcgctgcc ggccctctac tatgctggtc tctggtccgg cacaaactgg ccgacgatga
     1501 ttgccgcctg gaacacgctg atgctgcttt gggttgccgc tctcgtctgg agcaggggaa
     1561 agcagcggcg cggacgtgat tggttctggg cctggcccat agcccttgtg ctcatctcac
     1621 tcaactggat gggccccgaa ctgtgcagcc tcggcatcgt ctatcttcac cccttgatcg
     1681 cgttgtggtt cctggatcga caactgcgac gtacccgtcc tgaatggcta cctgcctacc
     1741 atcgatgcct ggcgcttttg ccttttgtca tcgtggcgat ctgctggcgc atgaacgggt
     1801 tggcaacgct ccaggatgac aacgggctcg cgtggcgcat aacacaacat gcgggcgctg
     1861 gacttctgcc gaacatctcc agcaacctgt tggtctccgt tcacgtcttt ttagagatgt
     1921 tgcattacct tgtttggctg gtggcgttgc cgctactcac gtcaactggc gcatgggact
     1981 taaagaaaat tccattagtg caacatccac gcggcttccc caggttgatc gccgccgtga
     2041 tagtgctggg gttactttta gtagcactac tctggctcgg ctttggtctt gattacggcg
     2101 ccacgaggga cctctatttt gccgttgcga ttgcccacgt attggcggaa gctcccttct
     2161 tgttacgcac gatgtgaaac tctacagcgg tttc
//
LOCUS       DASUEX010000022         5962 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_7198, whole genome shotgun sequence.
ACCESSION   DASUEX010000022 DASUEX010000000
VERSION     DASUEX010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5962
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7198"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..629
                     /locus_tag="VFV61_00355"
     CDS             <1..629
                     /locus_tag="VFV61_00355"
                     /inference="COORDINATES: protein motif:HMM:NF014895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HEX4898947.1"
                     /translation="ELECLALQRVRDDMGLVNVKTMIPFCRTVVEAERVVALMAEFGL
                     QQGKHELEIYAMCELPANVIYADEFLRVFDGYSIGSNDLTQLTLGLDRDSEMVAHLFD
                     ERDGAVEKMVTIAIEAAHRAGKKIGICGQAPSDYPEFARFLVEKGITSISLNPDTVLQ
                     TTHVILEAEKAAKPLPATSGERPILVLTEPRALDEAAAKHLVVNEPGG"
     gene            784..1170
                     /locus_tag="VFV61_00360"
     CDS             784..1170
                     /locus_tag="VFV61_00360"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX4898948.1"
                     /translation="MATLLIVDDDNNIRETLNDLLSEAHQCHTADRAEQALAYLEFES
                     YDVVLTDMAMPGLSGDQILKRVQMQHPDTPVIVISGNSDEDTARNLLNMGAFAYISKP
                     FKLEEVESAVVRAIARHQELKAQAKE"
     gene            complement(1333..2562)
                     /locus_tag="VFV61_00365"
     CDS             complement(1333..2562)
                     /locus_tag="VFV61_00365"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX4898949.1"
                     /translation="MIDFELTEDHKALVQTVKEFGQKEVAPFIKEWDEKQQYQPGILK
                     KMGELNLLGVCIPEQYGGAGFDYISLGLVCEELEAVDTFLRVVMSVHTGLNSLTLYSW
                     GTEAQKQKYLVPQAKGEKLATYGLTEPGAGSDVVGSRSTARREGDEYVLNGEKMWISL
                     ADMADNFLFFCWTDEEKRRKRDHSGMSCFIVERTMKGFSSGTIHGKLGIRAGNTGYFS
                     LQDVRVPKENLVGEEGEGFKIAMFALEQGRYTVAAGATGVIRASRDASIEYANTRETF
                     GTKIGNHQLVKQKIAEMEADYQMSHLLWLRAGYLKNEGKPNGKATSLAKWQATVRSEK
                     AASMAIEVHGANGYSNDYPVERYLRNCKAAIIYEGTRDIHTLMQADWALGLKHEKGAR
                     KTLPAWKPATSHVASGD"
     gene            2843..3739
                     /locus_tag="VFV61_00370"
     CDS             2843..3739
                     /locus_tag="VFV61_00370"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HEX4898950.1"
                     /translation="MPKKRNANTVRVGLIQMSCEQRPEANFKKAVARINDAAKKGAQV
                     ICLQELFRSQYFCQTEDIALFKLAETIPGPSTEALAKVAKQHRVVIVASLFEKRTAGI
                     YHNTAVIIDANGKLAGKYRKMHIPDDPLYYEKFYFTPGDLGFQTHETAVGKIGTLICW
                     DQWFPEAARLTALSGAQFLFYPTAIGWLPDEEEEMNLAQHSAWETIQRAHAIANGLYV
                     VAVNRVGREGKLNFWGQSFVADPFGRIIAKASADQEEVLLVDCDLNKIEETRQNWPFL
                     RDRRIDSYMSIGSRFLEPIVTK"
     gene            3739..4800
                     /locus_tag="VFV61_00375"
     CDS             3739..4800
                     /locus_tag="VFV61_00375"
                     /inference="COORDINATES: protein motif:HMM:NF016274.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HEX4898951.1"
                     /translation="MNQPTPAELGYFMPAEWERHAATWLSWPKDPLTWPERVPQVQEI
                     FLQMIAALSPHELVNLLVDDAETEALVRQRSVFPGAGNVRFHRLPTVDSWIRDYGPNF
                     LLNRKMPALAYNDWIFNAWGNKYEELKRDDSIPTQLETTLAVQRFEPGIVMEGGSIEV
                     NGAGIVMTTEQCLLNPNRNPKLDREGIEDYLKNYLDVQQVLWLGEGIVGDDTDGHIDD
                     IARFAGPDVIICALEEDPADANYELLQDNLRRVQQATDLNGRKFEVVTLPMPGVVAAS
                     NDNSPRALDRLPASYANFYIANNVVLAPIFGHANDKRALDTLQRVFPTRRIVAINCEP
                     LVWGMGTIHCVTQQQPAQV"
     gene            complement(4883..>5962)
                     /locus_tag="VFV61_00380"
     CDS             complement(4883..>5962)
                     /locus_tag="VFV61_00380"
                     /inference="COORDINATES: protein motif:HMM:NF014869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HEX4898952.1"
                     /translation="TAGVPNRRQYSAPRAFDYVIDAHSGDLVAELPRSHDATVKALDG
                     LKKSRTIGFSQNANGSFLHDPDLNLHTFDFGFGNVDALVSKLPGTYVSNPPDPWDGAA
                     VSAHANAADVLRFLSSVLKRNGVDNIGGPVRSSINCVEEDGTKEWDNATWYRKQMMYG
                     QSKKDGKLRSWALAKDIVAHELTHGITDYTARIEYLGESGALNESYSDIFGVIVANIG
                     QPDVKKWNWLIGEGLMDGEIALRDMRRPERFGQPSHMDDYQKLPKTEAGDWGGVHTNS
                     GIHNKAAYNMLTAKDAQGKYLLKPREVAALFYLALTQNLSRTSRFGDSRRGVELAARS
                     LFANKTNKQARLKAVAKAFDKVGIV"
BASE COUNT         1349 a         1668 c         1546 g         1399 t
ORIGIN      
        1 tcgagctcga atgtttggcg ctgcaacgcg ttcgcgatga catgggactc gtaaacgtaa
       61 agacgatgat tcccttctgc cggacggtcg tcgaagctga acgcgtggtg gcactgatgg
      121 ccgagtttgg tttgcagcaa ggaaagcacg agttagagat ctacgcgatg tgcgaactgc
      181 cggcgaacgt catctatgcc gatgaattcc tgcgggtgtt cgacggctac tcgattggct
      241 cgaatgacct tacccaactg acgctcggtc tggaccgcga ttccgaaatg gtggcgcact
      301 tgtttgatga acgtgatggc gccgttgaga agatggtgac gattgcgatt gaagcggcac
      361 accgagctgg taagaagatc ggcatctgcg gccaagcccc ttctgactat cctgagttcg
      421 ctcgtttcct tgttgagaaa ggcattacct cgatatcgct gaatcccgac acggtgctgc
      481 aaaccacgca cgtgattctc gaagctgaaa aggcagcgaa accgctgcca gcgacgagcg
      541 gcgaacgccc catcttggtc ctcacggaac cgagagcttt agatgaagca gcagccaaac
      601 atctggttgt gaatgagccg ggtggataaa gggaaacgag ccgcgacatg accttctcct
      661 ttgtcccgct ctccctttcc ccgtttccct caacattgcg tcgcatgatt tttcagggca
      721 acatagcctc ctgacgtact tcagattcct cattagcttc agggagcaac caggagaacc
      781 tcaatggcca cgctgttaat cgtcgacgac gataacaaca tccgcgaaac tctcaacgat
      841 cttctgagcg aggcgcacca gtgtcatacg gctgatcggg ccgaacaggc gcttgcttat
      901 ctggaatttg aaagttacga cgtggttctc accgacatgg ctatgcctgg actgagcggg
      961 gaccagatcc tcaaacgtgt tcagatgcag catcccgaca ccccggtaat cgtgatctct
     1021 ggaaactctg atgaggatac cgctagaaac ctactgaaca tgggggcgtt cgcctacatc
     1081 tcaaagccgt tcaaacttga agaggtggaa tccgctgttg tgcgtgctat tgctcgccat
     1141 caggaactca aggcacaagc gaaagagtaa ggccgagact cgttctggga tcttgggcct
     1201 cccgaccgca aaagcactta acgcgaaaac aatagccgca gattaaggcg gactgcacgg
     1261 atcagagagg ttagttctaa tccgcgtagt ctcattaacc cgcggccatt ttctttaatg
     1321 cttggtgatc ctttaatctc cggatgcaac gtgcgacgtt gccggtttcc acgctggcag
     1381 cgtcttccgc gccccctttt catgcttcaa gcccaatgcc caatcggctt gcatcaaggt
     1441 gtgaatgtcg cgggtgccct cgtaaatgat cgcggctttg cagttacgca gataacgctc
     1501 aaccgggtag tcgtttgagt aaccattcgc cccgtgaact tcgatagcca tcgaggctgc
     1561 cttttcactg cgcactgtcg cctgccactt ggctaagctt gtcgccttgc cattgggttt
     1621 gccctcgttc ttcaaatacc cggcccgcag ccaaagcaga tgcgacatct gatagtcagc
     1681 ttccatctcg gctattttct gtttcactag ttggtggttc ccaatcttgg tgccaaacgt
     1741 ctcgcgcgta tttgcatact cgattgaagc atcacgcgag gcgcgaatca cgccagtcgc
     1801 gccggcagcg acagtgtaac gaccctgctc gagagcaaac atcgcgatct tgaacccttc
     1861 accctcttca ccgacgagat tctcctttgg cacacggacg tcctgtaagg aaaagtagcc
     1921 ggtgttgccg gcacggatgc cgagcttgcc gtgaatcgtg cctgatgaga atcccttcat
     1981 cgtgcgttcg acgatgaaac acgacattcc actgtgatcc cgtttgcggc gcttttcttc
     2041 atcggtccag cagaagaaga ggaagttgtc tgccatatcg gccaaactga tccacatctt
     2101 ttcgccgttg agcacatact cgtcgccttc gcgccgcgca gtggagcgcg aaccgactac
     2161 atctgagccg gcgcctggtt ccgtcaaacc ataagttgcc agcttttcac ccttcgcctg
     2221 cggcaccagg tatttttgct tttgcgcttc cgtgccccac gagtagagcg ttaacgaatt
     2281 aagcccggtg tggacagaca tcacgacgcg cagaaaagta tcaacggctt ccagttcttc
     2341 gcagaccagg cccagcgaga tgtagtcgaa gcccgcgccg ccatattgtt cgggaataca
     2401 aacgccgaga aggttcagct cgcccatctt tttcagaatc ccgggctgat attgttgttt
     2461 ttcgtcccac tctttgatga aaggcgcgac ttccttctgg ccaaactcct taacagtttg
     2521 caccagggcc ttatggtctt cagttagctc aaaatcgatc atcttgaatc tcgctttggt
     2581 tttccggggt tatttaaaga agcgacatct tacaaagtcc aatgtccaac gtccaatgcc
     2641 caaagtcctt tgaaattgct gattggcgat tgggccattg ccgactacga acttctatgg
     2701 acggttgaat tgatggtttt gcgcaaaaag gagtttgggc tactgagctc cacctaatcg
     2761 gatcaatcgt aaatcggcat ttggcaatcg gcaatcgcaa atcgcactgg cctttggact
     2821 ttggactttc tatactccgc gcatgccgaa gaaacggaat gcaaacacgg tccgggttgg
     2881 actgatccag atgagttgcg agcagcggcc ggaagcaaac ttcaaaaaag cagtcgctcg
     2941 tattaacgac gccgcaaaga agggcgcgca agtgatctgc cttcaggaac ttttccgttc
     3001 tcagtacttc tgccaaaccg aagatatcgc gctcttcaaa ctcgccgaga ctattcccgg
     3061 tccttcgacc gaggcgctcg caaaagtcgc aaagcaacac agagtcgtaa tcgtggcctc
     3121 actgtttgag aagcgcaccg ccggcatcta ccacaacacc gctgtgatca tcgatgccaa
     3181 cggcaagctc gccggtaagt atcgcaagat gcacatcccc gacgatccgc tgtattacga
     3241 aaagttttac ttcacacccg gggatcttgg ttttcaaact cacgaaactg ctgtagggaa
     3301 aatcggaaca cttatatgct gggaccagtg gtttcctgaa gcagcacgtt tgaccgcttt
     3361 gagtggtgca cagtttttgt tctatcccac agcgatcggc tggttgcccg atgaagagga
     3421 agaaatgaat ctcgcgcaac actccgcctg ggagacgatc caacgcgccc atgcaatcgc
     3481 caacggcctt tatgttgttg ctgtgaatcg cgtcggccgc gaaggcaaac tcaatttctg
     3541 gggccaatcg tttgttgctg atcctttcgg acgcatcatc gccaaggctt cagccgatca
     3601 ggaagaggtg ctcttggtcg actgcgactt gaacaagatt gaggagacgc gtcagaactg
     3661 gcccttcctc cgcgatcggc gcattgattc ctacatgtca attggcagcc ggtttcttga
     3721 gcccatcgtt accaagtgat gaaccagccg actcccgccg aactcggtta tttcatgcct
     3781 gccgagtggg aacgccacgc cgcgacctgg ttgagctggc ctaaagatcc gcttacctgg
     3841 cccgaacgcg ttcctcaggt tcaagaaatc ttcctgcaga tgattgccgc cctctcacct
     3901 cacgagctcg taaaccttct ggtcgacgac gcggagactg aagccctggt gcggcagcgc
     3961 agtgtcttcc ccggcgccgg caacgttcgg tttcatcgac tgccgactgt cgactcctgg
     4021 attcgtgatt atggcccaaa cttcctgctc aacaggaaaa tgccagcgct tgcttacaac
     4081 gactggatct tcaatgcctg gggcaacaag tacgaggagt taaagcggga cgacagcatc
     4141 cccacacaac tcgaaacgac acttgccgtg caacgctttg agcccggtat cgtgatggaa
     4201 ggcggttcaa ttgaagtgaa tggcgccggc atcgtgatga caaccgaaca atgcctgctc
     4261 aatcccaacc gcaatccgaa actggatcgg gaaggcatcg aagactatct gaagaactat
     4321 ctggacgttc agcaggtgct ttggttagga gaaggaattg tcggcgatga cactgacggg
     4381 cacatcgatg acattgctcg gtttgccggg ccggacgtga ttatttgcgc tctggaagag
     4441 gatccagcgg acgccaacta cgagttattg caggacaatc tgcgccgcgt gcagcaagct
     4501 accgatctta atggtcgcaa gtttgaggtt gttacgttgc cgatgccggg agtcgttgcc
     4561 gcttcaaacg ataacagccc acgtgcattg gatcgcctac ctgcgagtta cgcaaacttc
     4621 tacatcgcga acaatgtggt gctggctccc atcttcggcc acgcaaatga caagcgcgcg
     4681 ttggacacgc tgcaaagagt gtttccaacg cgccgcattg ttgccattaa ttgcgaaccg
     4741 ctcgtctggg gcatgggaac cattcactgc gtgactcaac aacaaccggc tcaggtctag
     4801 tccaacgtcc aaggtccaaa gtcccatatc cgaacttttc gcgtgcggac tttggatgtt
     4861 ggactctgga ctttggtcct atttatacga tccccacttt atcaaacgcc ttcgcaacgg
     4921 ctttaagacg cgcctgcttg ttggttttgt ttgcgaaaag actccgcgcg gcaagttcga
     4981 caccgcgccg gctgtcgcca aaacgcgacg tgcgcgagag gttttgggtt agagccaaat
     5041 agaacagggc agccacttca cgaggcttca acaagtactt accctgcgca tcctttgcag
     5101 taagcatatt gtaagcagcc ttgttgtgaa tgccgctgtt cgtgtgcaca ccaccccaat
     5161 cgccggcttc cgttttggga agcttctggt agtcgtccat gtgggaaggc tggccgaatc
     5221 gttcgggacg gcgcatatcg cgcagggcga tttcgccatc catcaagcct tcgccgataa
     5281 gccagttcca cttcttcacg tcgggctgac caatgttcgc gacgatcact ccaaagatgt
     5341 cagagtagga ttcattcagc gcacctgact ctccgagata ttcgatccga gcagtgtagt
     5401 cggttattcc atgggtcagt tcgtgagcaa caatgtcctt ggcaagtgcc caggaacgca
     5461 gcttgccgtc cttcttgctc tgcccgtaca tcatttgttt tcgataccag gtcgcgttgt
     5521 cccactcttt ggttccgtcc tcttcaacgc aattgatgct cgaccgaacc ggtccgccga
     5581 tgttgtccac tccatttcgt tttagaaccg aactgaggaa cctcaaaaca tctgcagcgt
     5641 tcgcatgagc gctaacagcc gccccatccc aggggtctgg cggattcgag acgtacgttc
     5701 ccggaagctt gctgacgaga gcatcgacat tgccaaagcc aaagtcgaag gtgtgcagat
     5761 tcagatccgg gtcgtgaaga aaagagccgt tggcgttttg cgagaacccg atagtgcgcg
     5821 acttcttcag tccgtcgagc gcttttacgg tagcgtcgtg ggaacgcggc aactcagcta
     5881 ccaagtcgcc cgagtgagca tcaatgacat agtcgaaggc ccgaggcgcg ctgtattgtc
     5941 tccgattcgg tacgcctgct gt
//
LOCUS       DASUEX010000023         2569 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_580243, whole genome shotgun sequence.
ACCESSION   DASUEX010000023 DASUEX010000000
VERSION     DASUEX010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2569
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_580243"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..872)
                     /locus_tag="VFV61_00385"
     CDS             complement(15..872)
                     /locus_tag="VFV61_00385"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HEX4898953.1"
                     /translation="MTQTLQSRERELEFSLRLAREAGAAILEFYRQPMEIEQKHSFND
                     REPVTEADRVANDLIVSSLQREFPDDGILAEESVDTSHRLDKERVWMIDPLDGTNGFI
                     DGNGDFAVQIGLVENGEAILGVVYQPLSGVMYRAVRGAGCWIERPNSTPERAAVSQQT
                     ELHRMRLAASRSHRSPRMDRVVTAFAVKEEVRRGSVGIKVGLICERQCDLYVHLSPRT
                     KQWDTCAPEVILHEAGGRLTDLFGQPLRYNVAEVQNRNGLVASNGVVHEEIISALAPL
                     LDEFGRVAV"
     gene            complement(900..2429)
                     /locus_tag="VFV61_00390"
     CDS             complement(900..2429)
                     /locus_tag="VFV61_00390"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HEX4898954.1"
                     /translation="MKRERTIDSPARKSLDISEADLTQLSAAINKLVTDYFAGVRERA
                     VFPQATAGDTTSKVGAHLSAVGEPLEKLLADCRAILDGSRHNGHPRFFGYVASPSTPI
                     GAYGDLIASALNSNVTSWRSGPAATEIERTVVRWLGSLISYSDDAHGLLTSGGSLANT
                     TALLIAQRHKANWEVATHGLWKQDTPMTIYASEQVHMSIPKAADILGFGREQVRTVAC
                     DQRLRLDVRSLRERLERDRDHGLEPFCVVASAGTVNTGAVDPLADIAKIAREFGIWFH
                     VDGAYGAPGSLDARKRPLFAGLELADSVSLDPHKWLYVPLDAGCLLFRDEAAARQAFT
                     AGDADYIKVLEENPEESFAFWDYGTELSRRFRALKIWLTLRYYGVERLAAAISDDNSL
                     AQYLAERVEQADDFELLAPVELSICCFRYAPKQLRERLAASEEKEQALLGSELDALNQ
                     RIMLTVQRGGAAYVSNATIQGKFALRACITNFRTTRDDIDRTLEIIRSAASRCSAVPV
                     D"
BASE COUNT          588 a          761 c          658 g          562 t
ORIGIN      
        1 caatcggcaa tccgctagac cgccacccgg ccaaactcat ccagtagcgg tgccagggca
       61 ctgataatct cttcatgcac gacgccgttg ctggcgacga gtccattacg gttctgaact
      121 tccgccacgt tatagcgcaa aggctgacca aacaaatccg tgaggcgccc gccagcctcg
      181 tgcaggatga cttcgggggc gcaggtgtcc cactgcttgg tgcgcggcga aagatgaaca
      241 tagagatcgc actgccgttc gcagattagc ccgactttta ttccgaccga accacgccgc
      301 acctcttcct taaccgcgaa cgccgtgacg acgcgatcca tacgcggact gcgatgcgac
      361 cggctggccg ccaacctcat ccggtgcaac tccgtctgct gggaaactgc agcgcgttca
      421 ggcgtggaat tcgggcgctc aatccaacaa cccgcgccgc gaactgcccg atacatgaca
      481 ccgctcaacg gttgatagac aactcccagg atggcttcgc cattttccac aagacctatt
      541 tgtacggcga aatcgccgtt gccatcgatg aagccattcg tgccgtcgag tggatcgatc
      601 atccagacgc gttccttatc aagccggtgc gaggtgtcaa ctgattcttc tgcaaggata
      661 ccgtcatcgg gaaactcacg ttgcaaggaa ctaacgatta gatcattggc cacacgatcg
      721 gcttccgtta ctggttcacg gtcattgaaa ctgtgcttct gctcaatttc catcggctgc
      781 cgataaaact ccagaatggc cgcgcccgcc tcgcgcgcca gccttaacga aaattctaac
      841 tcccgttcgc gcgattgtag ggtctgcgtc atctgctcct tcgatctcgc cctgagttat
      901 cagtccactg gcacagcaga gcaacgcgac gcagcactcc gaatgatttc caaagtcctg
      961 tcgatgtcgt cgcgcgtggt gcgaaagttt gtaatgcagg cacgcagagc aaacttcccc
     1021 tggattgtcg cattcgaaac atacgctgcg ccgcctcgtt gcaccgttag cattatccgt
     1081 tgattcaaag catccaactc gcttcccaga agcgcttgtt ctttttcctc agacgcggcc
     1141 agcctttctc gcagttgttt tggcgcatag cgaaaacaac agatactcaa ttccactggc
     1201 gccagcagtt caaaatcatc cgcctgttcg acgcgttctg cgaggtactg cgccagcgaa
     1261 ttatcatcac taatcgctgc cgccagccgt tcaactccgt agtagcgcaa cgtcagccag
     1321 attttcagcg cgcgaaaacg tcgcgacaac tctgttccat aatcccagaa ggcaaacgac
     1381 tcctcaggat tctcttcgag tactttgatg tagtctgcat cgcctgccgt aaacgcctgg
     1441 cgcgcggcag cctcatcgcg aaacaagaga cagccggcat cgagtggcac gtaaagccac
     1501 ttatgcggat caagcgagac cgaatccgca agttcgagtc cggcaaagag cggacgcttg
     1561 cgcgcatcca gactgccagg cgctccgtaa gcgccgtcaa catgaaacca gatgccaaac
     1621 tcgcgggcaa tcttcgcaat atctgctaac ggatcaacag caccagtgtt gacagttccg
     1681 gcgcttgcaa ccacgcaaaa cggttccaga ccgtggtcac gatcacgttc taaacgctcg
     1741 cgcagggagc gcacatcgag tcggagcctc tgatcacagg ctacggtgcg aacctgttcc
     1801 cggccaaagc ccagaatatc cgccgctttc gggatcgaca tatgcacttg ctctgaagca
     1861 tagatcgtca tgggtgtgtc ttgcttccaa aggccgtgtg tcgcgacttc ccagttggct
     1921 ttgtgccgct gtgcgatcag taacgcagtc gtattggcaa gggagccgcc gctggttaat
     1981 aaaccgtgag catcgtcact ataactgatg agtgatccga gccagcgcac aaccgttcgt
     2041 tctatctctg tggctgccgg gcctgagcgc cacgaggtga cgttagaatt tagcgctgat
     2101 gcgatcagat ctccgtaagc tccgatcggt gtagacggcg acgccacgta accaaaaaac
     2161 cgcggatgtc cgttatggcg gctcccatcc aaaattgccc gacagtcggc gagcaacttc
     2221 tcgagcggct cgcccaccgc ggaaagatga gcgccaactt ttgaggttgt atcgcccgcc
     2281 gtcgcctgtg ggaagacagc tcgttcccgg actcctgcga agtaatcagt gaccagctta
     2341 ttgatcgcgg cagagagttg agtgagatcg gcttctgaaa tatccagcga cttccgcgct
     2401 ggtgaatcta ttgtccgctc gcgcttcatt tgaatcagac tatacaaggc ttttgagtgc
     2461 gaaactaagc acgagccaca aaatcacaag aaattgcccg aagatgttta gtgttgatta
     2521 gtggagcagt ttttctgttc gtagagtgta ttcggtgagt attagtaga
//
LOCUS       DASUEX010000024         3551 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1378377, whole genome shotgun sequence.
ACCESSION   DASUEX010000024 DASUEX010000000
VERSION     DASUEX010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3551
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1378377"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..497
                     /locus_tag="VFV61_00395"
     CDS             <1..497
                     /locus_tag="VFV61_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4898955.1"
                     /translation="FTRNSQQPTNANQTEPALVADPNSQAVEPSPPPTGSAEQGIPAG
                     GITNNQVVDANANTNANANVAASPTPDENANPDLNLNSNENANSNSNRPATNPPLPSP
                     SRQVNANENPTAPPPAEKKSPVPNPTQVPSSEAPPLYRELIAARIKSLSRSSSSIEIS
                     ISKT"
     gene            complement(416..1453)
                     /gene="lpxK"
                     /locus_tag="VFV61_00400"
     CDS             complement(416..1453)
                     /gene="lpxK"
                     /locus_tag="VFV61_00400"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HEX4898956.1"
                     /translation="MKPSLVLSPLSVAYAAATRLRIAAYKKGLLKTTQLPVPVISIGN
                     ITVGGTGKTPLVALISRLLAHEGRKVCILTRGYGRKNATERILVSNGSEVLAHLDDAG
                     DEPLWLAENLKDVAAVLCDPDRAAAGHWAIEQLGANVLVLDDGFQHLQLARDLNLLAI
                     DATNPWGSGRLLPSGRLREAPRGAARADCAIITRAELTSTTQELKTKLQELLGERPIF
                     TSHMQTTGVRELSGRRVDPGSGIAQPVAAFCAVGNPDSFFQQLRREGLSLAHTSAFPD
                     HHQYTQAEITRLSDDAKRAGAASLITTAKDAVKLHEFEFSLPCYVLEIEISIDEEDRL
                     RDLIRAAISSR"
     gene            complement(1455..2768)
                     /locus_tag="VFV61_00405"
     CDS             complement(1455..2768)
                     /locus_tag="VFV61_00405"
                     /EC_number="2.4.99.12"
                     /EC_number="2.4.99.13"
                     /inference="COORDINATES: protein motif:HMM:NF016311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HEX4898957.1"
                     /translation="MYVVYSLLLTLGFLVLLPRFLFDALRHGKYVAGFSERLGKLPPL
                     KSTGRPVVWVHCVSVGESQAARPLIAGIRQRFPDHSIVVSTTTLTGQKLARDIFKNEA
                     ERVFYFPFDWGWTVRRSLNAIKPSSVLIMETEIWPRFFHECQSRGVSLAIVNGRLSSQ
                     SIRRYRWIGFFLKRVLRGLTLAVMQTKTDAARIIELGLDPARVAVSGNMKFDAGTFGA
                     SGSLAAEFRDRFGISATKPLMIAASTHAPEEEVILEALRQAKSPNLRLLLAPRHPERF
                     AEVASLLQRSGLRWTRRSSPPHWNDKLCDAILLDTIGELSSVYALATIVFVGGSISAT
                     GGHNILEPAAVGAAIITGPHTHNFAAITRAFLKAGAIAQLASGNNQESARALAGTINY
                     LLSHDTRRHELQTKAKELVEQNRGATERTIQFLAPLLFASLHDIP"
     gene            complement(2821..3384)
                     /gene="efp"
                     /locus_tag="VFV61_00410"
     CDS             complement(2821..3384)
                     /gene="efp"
                     /locus_tag="VFV61_00410"
                     /inference="COORDINATES: protein motif:HMM:NF001810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEX4898958.1"
                     /translation="MAIPATQIRRGMVIVFEGQPCKVVEFRHHTPGNLRAMVQTKLRN
                     IKTGSSFEHRFRSADTIERATLEQHEMEYLYSDGSHHHFMNTENFEQIALSEDELGDA
                     AQWLSPGLRLQTEFYDGAPVAIDLPPSMELTVTRTEPTMKGATVSNVNKPATLENGVT
                     ITVPPFVNEGDRIRVDPNEGRYIERAK"
BASE COUNT          828 a          976 c          953 g          794 t
ORIGIN      
        1 cgttcacccg taattctcag caaccgacga atgcaaatca aactgagccc gcacttgtcg
       61 ctgatccgaa cagtcaggca gttgagcctt cgccgccacc caccggatct gctgagcaag
      121 gaataccagc tggaggaatt actaacaatc aggttgtcga cgcgaatgcc aacacgaatg
      181 caaatgcgaa tgtcgccgcc tccccgacgc cggatgaaaa tgctaatccg gatctcaatc
      241 tgaacagcaa cgaaaacgcc aatagcaata gcaatcggcc agctacgaat cctccgctcc
      301 cgagtccttc gcgccaagtg aacgcgaatg agaatccgac ggcgccacca ccggctgaga
      361 aaaagtcacc agtaccaaac ccaactcagg tgccaagctc cgaagcacca ccactctatc
      421 gcgagctgat tgcagctcga atcaaatctc tcagtcggtc ctcttcgtcg atcgagatct
      481 caatctccaa aacataacaa ggcaggctaa actcaaattc atgtaatttg acggcgtcct
      541 ttgcggtagt tattaaagag gctgcaccgg cccgtttagc gtcgtccgag aggcgagtga
      601 tctctgcttg tgtgtattga tgatggtcgg gaaacgcgct cgtatgagct aacgagagtc
      661 cctccctgcg gagctgttgg aaaaaggagt cagggtttcc gacagcacaa aatgcggcga
      721 caggttgagc gattccgctc ccagggtcga cccggcggcc gcttaattct ctgacgcctg
      781 ttgtttgcat gtgcgacgtg aagattggcc gctctccgag cagttcttga agtttcgttt
      841 tgagttcttg agtggtggag gtcaattcgg ctcgcgtaat gatggcgcaa tctgcacgtg
      901 ccgcgccccg cggcgcttca cgcaaccttc ccgagggaag cagtcgccca ctgccccagg
      961 gattggtggc gtcgattgca aggagattaa ggtcgcgcgc aagttgcaga tgctggaagc
     1021 catcatccaa aaccaaaaca ttggcgccaa gttgttcaat cgcccagtga ccggcagcgg
     1081 ctcgatcagg atcgcacaac accgccgcca catcttttaa gttttcggcc agccaaagcg
     1141 gttcatcacc agcatcgtct aaatgggcca gcacttctga cccgttcgag acgagtattc
     1201 gttccgttgc gtttttccgt ccatagccgc gcgtgagaat acagaccttg cgtccctcgt
     1261 gagccagcag gcgactgatg agtgcgacca aaggagtctt cccagtcccg ccaactgtga
     1321 tgttgccaat gctgataact ggaaccggca actgagttgt tttcagcaac ccctttttgt
     1381 acgcagcgat tcgcaaccgc gttgctgcgg cgtaggcgac tgagagcggt gataacacca
     1441 gacttggctt catctcaggg aatgtcatgc aggctggcaa agagtagtgg cgcgagaaac
     1501 tgaattgttc gctccgtcgc tccccggttt tgctctacta attcctttgc tttggtttgc
     1561 aattcgtggc gacgcgtatc atggctcagc aggtagttaa ttgttcccgc cagcgccctt
     1621 gcagattcct gattgttgcc cgaggccagt tgcgcaattg ccccggcctt aaggaacgct
     1681 cgcgtgatcg cagcgaagtt gtgcgtgtgc gggcccgtaa ttattgccgc gccgactgcc
     1741 gcaggttcga gaatgttgtg cccgccggtt gctgaaatac taccgcctac aaaaacaatc
     1801 gttgctaaag cgtaaaccga ggaaagctcg ccaatcgtat ccagcagaat tgcgtcacag
     1861 agtttgtcat tccagtgcgg cggggaactg cgacgagtcc agcgcaagcc cgatcgctgt
     1921 aaaagggaag ccacctcggc gaagcgttcc ggatgacgtg gtgcgagcag cagccgcagg
     1981 ttgggacttt tcgcctgccg caacgcctcg aggattacct cctcttcggg cgcgtgcgtg
     2041 ctggcagcaa tcattagtgg cttagttgcg gagatgccaa aacgatcacg aaactcagcg
     2101 gcgagtgaac ccgacgcacc aaaggtaccg gcgtcgaatt tcatatttcc cgaaacagca
     2161 acgcgcgccg gatccaatcc cagttcgata atccgagcag catccgtctt agtctgcatt
     2221 acggcaagag tcaatccccg aagcacccgt ttgagaaaga acccgatcca cctgtaacgg
     2281 cgaatcgact gtgaggatag ccgaccgtta actattgcca gacttactcc ccgcgactgg
     2341 cattcatgaa agaaacgcgg ccaaatctcc gtttccatga ttaataccga actcggctta
     2401 atcgcattca aagagcggcg caccgtccag ccccagtcga atggaaagta aaacactcgc
     2461 tctgcttcat tcttgaagat atcgcgggcg agcttttgtc cggtaagagt tgtggtcgaa
     2521 actacaattg agtgatcggg aaatcgttgg cgaattccgg caattagagg tcgcgctgcc
     2581 tgcgactcgc cgactgagac acagtgaacc cagacaactg gccgacctgt cgatttgagt
     2641 ggagggagtt ttcccagtcg ctcactaaat cctgcaacat acttgccgtg gcggagagcg
     2701 tcgaagagaa agcggggaag gagaaccaga aagccgagag taagtagcag gctgtagacg
     2761 acgtacatag gtgatgggcg agggctgggg aaaccctcgg caagctagat cacctgacgg
     2821 ttattttgcg cgttcaatat agcgtccttc gttaggatct acgcgaatcc gatcgccttc
     2881 attaacaaat ggaggcacgg taatcgttac gccattttca agggtggcgg gtttgtttac
     2941 attcgaaact gttgcacctt tcatggtcgg ctcggtgcgc gttaccgtga gctccatcga
     3001 agggggaagg tcaatggcga ccggagcgcc gtcatagaat tccgtttgca gccgcaatcc
     3061 tgggctcaac cattgagccg cgtcaccgag ttcgtcctca cttaacgcga tctgctcaaa
     3121 attttcggta ttcatgaagt ggtgatggga accgtcagaa taaaggtatt ccatctcgtg
     3181 ctgctcaagg gtcgcgcgct cgatggtgtc agcagagcga aagcgatgct cgaatgagga
     3241 gccagtctta atattgcgca acttggtctg caccatcgcc cgcaagttgc ccggggtgtg
     3301 gtgtctaaac tctactactt tgcacggttg tccttcgaaa acgatgacca taccgcgtcg
     3361 aatttgggtt gccggaatcg ccataaacta tctctcagtc tccatctaac attgtgttcg
     3421 agtctacacg gtgagggttt ttgctcctca acgaatcgcg tagtctagcg agagccctgt
     3481 agttttgtcc agcacggtcc tccttcggca gtgctccgtc agggcgttgc tcctgatcgg
     3541 ttactgttcc a
//
LOCUS       DASUEX010000025         3414 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_122613, whole genome shotgun sequence.
ACCESSION   DASUEX010000025 DASUEX010000000
VERSION     DASUEX010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3414
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122613"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(270..758)
                     /gene="greA"
                     /locus_tag="VFV61_00415"
     CDS             complement(270..758)
                     /gene="greA"
                     /locus_tag="VFV61_00415"
                     /inference="COORDINATES: protein motif:HMM:TIGR01462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEX4898959.1"
                     /translation="MSTDIKQKLQGELDELEAELRIHLPKEIKRALEFGDLRENSEYH
                     AALDRQNMVKARIRELRQRISEIASIDVSKVPRDKASYGSTLILYDGERDEEVTYRLV
                     TPEESDPQQGFISTTSPVGRSLMGKQPGDEVVVTTPAGARNFEIRQLTTLHDEQAPGS
                     AD"
     gene            complement(794..1906)
                     /locus_tag="VFV61_00420"
     CDS             complement(794..1906)
                     /locus_tag="VFV61_00420"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HEX4898960.1"
                     /translation="MQKTIGILTGGGDAPGLNAVIRAVVKTAIGEFGMRVIGIEDGFE
                     GLLGETQTRPLSHGDVRGLLPRGGTILGTRNRGRFVEHSANGVSSTSEAVYQEAAANI
                     KRLGISALLVLGGEGTLTIAAEFDRRGIPVVGVPKTIDNDLACTELTFGFVTALDIAT
                     EALDRLHTTAESHDRVMILEVMGRNAGWIALHSGIAGGADVILIPEIPFSMESVAKKI
                     RAREEGGSNFSIIVTAEGAIESGHQQIFQDSGDLQHAPRLGGIAQYCRSQLEALTGKE
                     TRCVVLGHLQRGGRPNAFDRMLATTYGACAVRAFVEGKHGVMVALQAGDVVTVPISEA
                     VANVKTVPPNGQMVTTARDTGISFGAPDEAKYHRLV"
     gene            complement(1921..2175)
                     /locus_tag="VFV61_00425"
     CDS             complement(1921..2175)
                     /locus_tag="VFV61_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4898961.1"
                     /translation="MKGIPTVLITVSPEVSAQMRPPRALYPNGFKIGNSLGKPGMRAL
                     QKTVLREALSLLTLDTRPGSVVTRDFPEYGEYHEPPHVRK"
     gene            complement(2224..2649)
                     /locus_tag="VFV61_00430"
     CDS             complement(2224..2649)
                     /locus_tag="VFV61_00430"
                     /inference="COORDINATES: protein motif:HMM:NF019003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase
                     selenoprotein B family protein"
                     /protein_id="HEX4898962.1"
                     /translation="MTIARKCVPFTPFDGELAKSTIAIVTAGGVHRKDQEPFNIADEL
                     GDLGYRVFAEDVESTDLKVTHHHYDHADADQDINVVFPIDVLRDLQAEGFIRNLAKKH
                     IGYMGYTQRLKAMYEGTAREIANEIDKGSRADAVVLTGG"
     gene            complement(2784..3248)
                     /locus_tag="VFV61_00435"
     CDS             complement(2784..3248)
                     /locus_tag="VFV61_00435"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(3108..3110),aa:Sec)
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX4898963.1"
                     /translation="MPEVGDMAPDFELPSNLEKGKKVRLSDFRGKKNVVMAFYPLAWT
                     PVUTSQMPAYEADLERFEGYDAQVLGISVDSVPCNTAWAKSLGGLSYDLLSDFEPKGE
                     VAKLFGAYRQTDGISERAIFVVDKDGKLVFKDIHDISDQPDNEEVLEVLRKL"
BASE COUNT          730 a          963 c          896 g          825 t
ORIGIN      
        1 acagaccgca gcctacgttc agggaaacgc caatcagggt cagtatgttc gggttgatat
       61 gaccaacagc cagccaacga accatcgcat cgatgattct ctgcgcgccg cgaccgatac
      121 catccacaaa catctgctgg aaaagtctcc ttattgattc cctgattgca aaaagccgcg
      181 gggagataag cggcggattt taggaagcag ggcgacgcgc tgtcaaggtt tacgggactc
      241 cgggttaatg aaccgtatgg tcgaacttct cagtcggcac tgccaggcgc ctgctcgtca
      301 tgaagggttg ttaactggcg gatctcaaag ttgcgcgccc ctgccggagt cgtgacaaca
      361 acttcatcgc ccggttgttt gcccatcagg ctccggccga cgggagaagt ggtcgagata
      421 aaaccttgct gcggatcact ttcttccggc gtgacgaggc ggtatgtgac ttcttcgtca
      481 cgttcgccat cgtaaaggat caaggttgaa ccatatgatg ccttgtcacg cggcacctta
      541 ctgacatcga tagaggcaat ctcgctgatg cgctggcgca gctccctgat gcgcgccttg
      601 accatgtttt gccgatcgag tgcggcgtga tattccgagt tctcgcgcaa atcgccaaac
      661 tcgagcgcgc gtttgatttc cttcggcagg tgaatgcgca actccgcttc cagctcgtcg
      721 agttcgcctt gcagtttttg ctttatgtca gtagacaact aaacctctcc cctcgtgttt
      781 gtagagacac attctagacc aaacggtgat acttggcctc atccggagcg ccaaacgaga
      841 ttcccgtatc gcgagctgtc gttaccatct ggccattggg cggaaccgtt ttcacattcg
      901 ccacggcttc ggagatgggc acggtcacca cgtcgccggc ttgcaacgcc accatcaccc
      961 catgttttcc ctctacaaaa gctcgcacgg cgcaggcgcc gtaggtggtt gccagcatgc
     1021 gatcgaaagc attgggccgg ccgccgcgct gcaggtgacc taagactacg caacgagtct
     1081 cttttcccgt gagtgcctcg agttgactgc gacagtactg ggcgataccg ccgagtcggg
     1141 gtgcgtgttg caggtcgccg ctgtcctgaa aaatttgctg atgaccgctt tcgatcgctc
     1201 cctctgcggt aacgatgatg ctgaaatttg agccaccctc ttcgcgagcg cgaatctttt
     1261 tggcgaccga ttccattgag aaagggatct ccggaatcag aatgacgtca gcgcctccag
     1321 ctataccaga gtgcagggct atccagcctg cgttgcgtcc catgacctcc agaatcatga
     1381 cgcggtcgtg cgactcggcg gttgtgtgca gtcgatcgag cgcctccgta gcgatgtcca
     1441 atgcggtaac aaaaccgaat gtcagctcag tgcaggccag gtcgttatcg atggtcttgg
     1501 gcacgccgac caccggaatg ccccgacgat cgaattcagc ggcgatggtg agcgtgcctt
     1561 cgccacccag aaccagaagg gcactgatgc ccagtcgttt gatattcgcg gcagcttcct
     1621 gatagaccgc ttccgacgtt gacgagacgc cgttcgctga gtgctctaca aatctcccgc
     1681 gattgcgcgt gcccagaatc gtgccacctc gaggcagcag gccgcgcacg tcaccgtgcg
     1741 agaggggccg cgtctgcgtt tcgccgagca gcccttcaaa tccgtcttcg atgccgatga
     1801 cacgcatgcc aaattcgcca atcgcagttt tcacgacagc gcgaattacg gcattcaaac
     1861 ctggcgcgtc gccgccccct gtgagaattc cgatagtttt ctgcatcact tttttgctgg
     1921 ctactttctt acgtgcggag gctcgtgata ttcgccatat tcgggaaagt cgcgggtgac
     1981 aactgacccc ggtcgcgtat caagcgtcag cagcgacagc gcttctcgca gaactgtttt
     2041 ctgtaaggca cgcattcccg gtttgccgag actgtttccg atcttgaaac catttggata
     2101 caaggcccgt ggtggacgca tttgggcgga aacttcggga ctgactgtga tcaaaaccgt
     2161 gggaatccct ttcatctcaa tttctcgctg aaccgcgacg accgtgcggt gacaaacgcc
     2221 tggtcaacct ccagtaagaa ccacagcatc cgcgcgggag cctttatcga tttcattggc
     2281 aatttcgcgc gccgtccctt cgtacattgc cttgagccgc tgggtgtatc ccatgtagcc
     2341 aatgtgcttc ttagcaaggt tccgaataaa gccttcggct tgcaggtctc gcaacacgtc
     2401 gatgggaaac actacgttga tgtcttgatc ggcgtcggca tggtcgtaat gatggtgggt
     2461 aactttcaga tcggtcgact caacatcttc ggcgaatact cgatacccca ggtctcccag
     2521 ttcatctgcg atgttgaaag gttcttgatc tttgcgatgg acaccaccgg cagtaacgat
     2581 cgcgatggta cttttcgcca actcgccgtc gaacggtgtg aacggaacgc actttcgggc
     2641 aatggtcata gataagtctc aggtctcaag tttcttcagc gatcgttaat tccaactcta
     2701 gtccgcgaga ccgcggttcc aactcttgag actcaagttt gggaacttga gacttgagac
     2761 ctgagacgtg atacccgaga tcgtcaaagt ttcctcaaaa cttcaagcac ttcttcgttg
     2821 tccggctgat cagaaatgtc gtggatatcc ttgaagacga gcttgccgtc tttgtccacg
     2881 acgaatatcg ctcgttcgct gataccatca gtttgccgat acgcgccaaa tagcttggcc
     2941 acttcacctt taggttcgaa gtcgctcaag agatcgtaag agagtcctcc cagactcttt
     3001 gcccatgctg tgttgcacgg cacgctgtca acggatatgc ccaggacctg agcatcgtag
     3061 ccttcaaagc gttcgaggtc ggcctcgtac gccggcatct ggctggttca aaccggtgtc
     3121 caggcgagcg gataaaatgc cattactaca tttttcttac cccgaaaatc tgagagcctg
     3181 accttcttcc ctttttccag gttgcttggc aactcaaaat ccggagccat atctccaact
     3241 tcaggcatta gagaagtcct ccttcgtttt actgatttcg aaaatggggg tcacaccaag
     3301 atagttgacg ctagagggta tttcaagcct cggtaacgaa agtctgaaga ttgaccatgt
     3361 tttgtctgct ggcgtagcat gcgagtgctt gccacacttt gtgaaggcta aatt
//
LOCUS       DASUEX010000026         6425 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_8423, whole genome shotgun sequence.
ACCESSION   DASUEX010000026 DASUEX010000000
VERSION     DASUEX010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6425
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8423"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..720)
                     /locus_tag="VFV61_00440"
     CDS             complement(<1..720)
                     /locus_tag="VFV61_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4898964.1"
                     /translation="MRKNCTLLILILLSATTVAWGQTATTGTAAAQAGDAGITPNRVI
                     GEVKAIDATAKQLIVKTDAGALVTATLSDATQFMRVAPGETNLANAAKITFADLAEGD
                     RVMAMGKVSDDKKSVPTRAVIVMTKADIAKKQDAERAEWRRRGILGVVSALNPAAKEI
                     TITSRSLAGVQSIIIRVSDKVEMRRYAPDSIKFGDAKQSTFAELQVGDQLRALGERAP
                     DGTRFTPEKVVTGAFRTVVGTV"
     gene            complement(779..1183)
                     /locus_tag="VFV61_00445"
     CDS             complement(779..1183)
                     /locus_tag="VFV61_00445"
                     /inference="COORDINATES: protein motif:HMM:NF025173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4898965.1"
                     /translation="MNEKSNSRMKTWLVLVAVFLLGAVTGVGLSGVYRSKANGSFREG
                     RGRDHQAMFERIRNDLDLNAEQSKEMQRVLDETAGEFRTLRGELRPRYEELRLKARGR
                     MRSILTAEQQKKFDSLMAEIDARRQNAGGQPR"
     gene            complement(1180..1605)
                     /locus_tag="VFV61_00450"
     CDS             complement(1180..1605)
                     /locus_tag="VFV61_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4898966.1"
                     /translation="MTKERDAISDERLDRAGRIITKAAVLSEAEAGEIVAAPFLFARI
                     QARIEAERQPSDGGIWTAFWSISKRAIPAMIIVAALSSGLSLYFTGNKNQPTAFSVDA
                     YLGTNESGVENLVFAERRPLTRDEVLTAIISKDDRELGR"
     gene            complement(1702..2298)
                     /locus_tag="VFV61_00455"
     CDS             complement(1702..2298)
                     /locus_tag="VFV61_00455"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX4898967.1"
                     /translation="MESSHDPISKKTSDADLVVAVVQGEVAAFGLLFERHRQRVAAIA
                     GRFFQQAPQIEDVVQETFIKAYFGLANFSQYKVDSFAHWLARIAFNSCYDELRKQSRS
                     HERSLSDFNDAERQELRRLTSGSVVTTVESVAIDRDLAHKLLRLLSAEDRLVLVLLDV
                     EGLTVAEIARLMDWSSAKVKIRAFRARTDLRRLLKKFL"
     gene            complement(2377..3372)
                     /locus_tag="VFV61_00460"
     CDS             complement(2377..3372)
                     /locus_tag="VFV61_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEX4898968.1"
                     /translation="MTIIATAEPFFAQANPEQVLLRFVTRTSLLFVWVLVAVVAIYAQ
                     APAPAGNVAETTYVVEGVSDTMAYGLGRSVAVNGTVKDGAISLGGDVIVAGRVDGDVA
                     AIGGSVIQLKGASIGGDVIVVGGTYRHDDGIPNRSPGKSTILYAGYEQELKNLIRNPT
                     EMVSPRWSKTYVGLRILALLFWFVVSLLVTAVIPETISRGIARLQLTMGRVAIIGVIG
                     AVIIGAGIPFSLHFLPTPLNALVGLLAFGLIVVAALFGRVVVYAATGRWLQRKLFPAS
                     ANSETVALLLGTAFWITLASLPYLWPLVFSVLIVTSLGLALTARYGISWKQAKAS"
     gene            complement(3388..3987)
                     /locus_tag="VFV61_00465"
     CDS             complement(3388..3987)
                     /locus_tag="VFV61_00465"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX4898969.1"
                     /translation="MEISKHNSEGEHIPAQVEAYLDGQLDVATTSQFEAHVAACQPCR
                     MELNAQRQFLCELDSVLSAPKEFFIPKDFARIVSARAESDMRGVRTGGERRRALLVCL
                     ALGLTAFALLGAAAGTSLLVSAALLTNKVLGVLSLLWTTLHDVFIGTTIISRVIVRML
                     LPESPLKNFAMLTLLALAVASLSHLIISYHRRGQMRLSR"
     gene            complement(4007..4618)
                     /locus_tag="VFV61_00470"
     CDS             complement(4007..4618)
                     /locus_tag="VFV61_00470"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX4898970.1"
                     /translation="MSMTASPALWKEFGAVTDRELVATAATGAEGSFEELVRRYQRPI
                     SSYVYRMVGDYEAALDLTQEIFIKVYGSLSRYRAEFKFSTWIYKIAHNCAVDHLRRNA
                     GRERSLLGGLEGDHYELPIESGNLSPEQESERRERRLEIEFVVRSLPAAYRELIALRH
                     SQDLTYEEIVEVTGLPLGTVKNRLFRARELMRQQFVERGITGM"
     gene            complement(4849..5793)
                     /locus_tag="VFV61_00475"
     CDS             complement(4849..5793)
                     /locus_tag="VFV61_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4898971.1"
                     /translation="MPSPLSKLLHPWYPATALGLEKGLASVVELERRGNAGSLRRAAT
                     VKLPESLISPSFDESNIEDHSELAAAIRELAASAGMLKQKRWSVSLPEATARTLILTL
                     EPHSGSGSELEEVLRWKMDRGFGVPLDELSISRERLAKDSQGRDRYLVVATRLAVLAE
                     YEHLFGMLGWRAGLVLPRHMGESQWLTRNGVNGDSLLLSSTNEGFTAVVYRNKQPLIF
                     RSIECDPDEREDELYRLLLFYRDRRVADSEFEQPLSRFLVTGAGFDKARANQIVNETT
                     GGDLRPLDPEDLGLRLPTTGLDFDAIAAPAGLATLSWS"
     gene            complement(5968..>6425)
                     /locus_tag="VFV61_00480"
     CDS             complement(5968..>6425)
                     /locus_tag="VFV61_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX4898972.1"
                     /translation="RSFGTSPRPRVPASPRLRIPSSRCLMLAVGFTLLELMIVITIVI
                     ILAAIALPQYQRTILHAREAVLRDDLYKMRTLLDQYAADKGKLPQSLEDLIAGHYMRE
                     LPVDPITGEKDWTVETGEDPNSTSGESGVINVRSASPDTSSEGTPYSEW"
BASE COUNT         1491 a         1864 c         1675 g         1395 t
ORIGIN      
        1 gacggttcca acaactgttc taaaagctcc ggtgaccact ttctccggcg tgaaccgcgt
       61 accatccggg gcccgctcgc ctaacgctcg caactgatcg cccacctgca attctgcaaa
      121 cgtgctctgc ttcgcgtcgc caaacttgat cgaatcgggc gcataacgcc gcatctccac
      181 tttgtcgcta acgcgaatga ttattgactg cacaccggcc agggaacgac tggtaatagt
      241 gatctcctta gcagcgggat taagcgccga aacgacaccg aggattccgc gccggcgcca
      301 ttcagctctt tccgcgtcct gcttcttcgc gatgtcggct tttgtcatga cgatgacagc
      361 acgtgtaggc acggacttct tgtcatctga aaccttaccc atcgccatca cgcgatcgcc
      421 ctcggccaga tcggcgaagg tgatcttcgc ggcgtttgcc agattggttt caccgggcgc
      481 tacgcgcatg aattgcgtcg cgtcactaag cgtggccgtg accagtgctc cagcgtcagt
      541 cttgactatt agctgtttgg cggtcgcatc aatcgccttc acttcaccga tcacgcgatt
      601 tggcgtgata cccgcatcgc cagcttgcgc tgccgctgtt ccggtcgtcg cagtctgacc
      661 ccaggcaaca gtcgtcgcgc tcagcaaaat caaaataagt agtgtgcagt tctttctcat
      721 cttcttttct gtacccctga ataaactaac caaccagtat gcgaataaat aaggccactc
      781 aacgcggctg cccaccggcg ttttgccggc gcgcgtctat ctcagccatt aacgagtcaa
      841 acttcttttg ctgttccgct gtcaggatgg agcgcatgcg gccgcgtgct tttagacgca
      901 actcttcata ccttggtctt aactcaccgc gcagcgtgcg aaattcaccg gccgtttcgt
      961 ccagcactct ctgcatctct ttagactgtt cagcattgag gtcgagatcg ttccgaattc
     1021 tttcgaacat tgcctggtga tcccggccac gcccttcgcg gaaagaacca ttggccttcg
     1081 atcgataaac accactgaga cctacacccg tgacagcacc aagtagaaaa actgctacca
     1141 gcaccagcca ggttttcatg cgactattcg acttttcgtt catctgccga gctccctgtc
     1201 gtctttgcta ataatggcgg tcagaacttc atccctggta agtggccgtc tttcagcaaa
     1261 gaccagattc tcgacgcctg attcgttagt tccgaggtag gcgtctacgc taaacgctgt
     1321 cggctggttt ttgttcccgg tgaagtacag ggaaagcccc gagctgagcg ctgcaacgat
     1381 gatcatcgcc ggaatagcac gctttgagat cgaccagaac gcggtccaga ttcctccatc
     1441 cgagggctgc cgttctgcct caatacgcgc ctgaatgcgc gcaaacagaa atggcgccgc
     1501 gacaatttca ccggcttcgg cttcagaaag gacggccgct ttggtaatga tgcggccggc
     1561 gcgatcgagg cgttcatccg aaattgcatc tctttctttg gtcatttgct tgattggaga
     1621 aactgcggac agtccggtgg aaacccggtc tccactctaa aaacgctggt caactccgag
     1681 cggttacaac tatttgaaaa cttagaggaa ttttttgagc agacggcgga gatctgtccg
     1741 ggcccgaaag gctcgaatct tcaccttcgc tgaggaccag tccatcaatc gcgcaatctc
     1801 tgcgactgtc agtccctcaa catcgagcag aaccagtacg agccgatctt cagcgctgag
     1861 caaacgcagt aacttgtgcg ccagatcgcg atcgattgcg actgactcga cagtcgttac
     1921 tacgctgccg gaagttagcc tgcggagttc ctgccgctcg gcatcgttga aatcgcttag
     1981 acttcgctcg tggctgcgac tctgttttct aagttcatcg tagcaactat tgaacgctat
     2041 ccgcgccagc caatgtgcga aggaatcaac cttgtactgt gagaaattgg cgagtccgaa
     2101 ataagccttg atgaacgtct cctgcacgac gtcttcaatc tgtggtgctt gttgaaaaaa
     2161 tctgcccgcg atcgcagcta ctcgctggcg gtgacgttcg aaaagcaatc cgaacgctgc
     2221 cacttcgccc tggacaacgg caaccactag atctgcgtcg ctggttttct tgctaatcgg
     2281 atcgtgggat gactccatct ggggatcaga aggctacgac aggatcggag agttgggcta
     2341 acgactggga cctaccaatt cgtaatcagc aatcgcttag ctagccttcg cttgtttcca
     2401 gctaatgccg tagcgcgcgg tgagggccag gccgaggctg gtaacgatca gcacggagaa
     2461 aaccagcggc cagaggtacg gcagtgaagc cagcgtgatc caaaaggccg ttcccagcag
     2521 cagggcaacg gtttctgaat tggcactcgc cggaaagagt ttcctctgca gccagcggcc
     2581 cgtggccgca taaaccacga ctcttccaaa gagtgccgcc accactatga ggccgaacgc
     2641 cagcagtccg acgagcgcgt tgagcggggt cgggaggaaa tgaagactga aaggaatgcc
     2701 cgcgccgatt atcactgcgc cgatgacgcc gatgattgcc acgcggccca tcgttaattg
     2761 caagcgtgcg atcccgcggc tgatcgtttc gggaatgact gccgtcacca ggagagacac
     2821 cacaaaccag aagagcaggg cgagaattcg cagccctaca tatgtcttag accagcgcgg
     2881 gctaaccatt tcggtgggat tgcgaatcag gttttttaac tcctgttcgt aacccgcata
     2941 aagaattgtg ctcttgcccg gactgcgatt cggaatgccg tcgtcgtgac gataagtgcc
     3001 acccacaacg atcacgtcac cgccaatgct ggctcccttt agctggatca ccgaaccccc
     3061 gattgccgcc acgtctccgt caactctacc ggctacaatc acatctccac ccaaagagat
     3121 cgcgccatcc ttgactgtcc cgttgaccgc cactgaacgc cccagaccgt aggccatcgt
     3181 gtcgctcacg ccttcaacga cgtaagttgt ttcggcaaca ttacccgccg gcgcaggcgc
     3241 ttgcgcgtag atggccacca cggcaaccaa cacccaaaca aacagcaggc tggtacgcgt
     3301 cacaaagcgc agcaggacct gctcggggtt tgcttgagcg aaaaatggtt ctgctgttgc
     3361 gatgatcgtc ataccaatga acacgcctta tctggaaagg cgcatttggc cgcggcgatg
     3421 ataactaatg attaggtgag acagtgatgc gaccgcgagt gcaagcagcg tcagcattgc
     3481 aaagttcttt agtggggact cgggcaaaag cattcttact atcacgcggg aaatgatcgt
     3541 tgtgccgata aacacatcgt gcagagttgt ccagagcaaa ctcaggacac ccagaacttt
     3601 gtttgtcagc agggcggcgc taaccagcaa cgacgtgccg gcagcggcgc ccagcaacgc
     3661 aaaagcggta agacctaatg ccagacaaac taaaagagcc cgccgacgtt cgcctccggt
     3721 acgcacgcca cgcatgtcac tctccgcgcg cgctgacaca atgcgggcaa agtctttggg
     3781 tatgaaaaat tctttgggag cagacagaac ggaatcaagc tcgcagagga attgacgttg
     3841 cgcgtttaac tccatgcggc acggctggca agcagcaaca tgtgcttcga actgactagt
     3901 tgtcgcgacg tcgagttgac cgtcgagata agcctcgacc tgagcgggaa tgtgttcacc
     3961 ctcagaattg tgtttgctga tttccatgtg ctgggtaatc gcgttgttac atccccgtga
     4021 tgccgcgctc gacaaattgc tgccgcatca gctcgcgggc gcgaaacagc cggttcttca
     4081 ctgttcccaa aggcaagcca gtcacttcta caatctcttc gtaagtaaga tcctgcgaat
     4141 gccgcaaggc aatcaactca cgataagccg ccgggagcga gcggaccaca aactcgattt
     4201 ccagtctgcg ttcgcgccgt tcactctcct gttccggact caggttcccg ctctcgattg
     4261 gcagctcgta atggtcgcct tccaaaccgc cgagcagtga tcgctcgcgt ccggcattgc
     4321 ggcgcagatg atcgactgcg cagttgtgcg cgatcttata aatccaggtc gaaaacttaa
     4381 actctgcccg gtagcgagaa agcgacccat acaccttaat gaagatttcc tgggtcagat
     4441 ccagcgccgc ctcgtaatcg ccgaccatcc gatagacata agaggaaatg ggacgctggt
     4501 agcgtcgcac cagctcctca aaacttcctt cagcacccgt ggcggcagtg gccacgagct
     4561 cgcgatcggt gaccgcgcca aattctttcc aaagtgcggg agatgcagtc atgctcatga
     4621 tgctcagttt ttacgagcgc caaaacggat aagtttcaaa agacccttaa cgtctcaggt
     4681 cacaggtttc aggtttcagg tttcgttaag tctccggagt cgtgggtttc gcaataatcc
     4741 tcaatagttg cctgaaacat gcgcaaaact cgggacacga acctctaatc aagaaagcga
     4801 cctgaaactt gagacctgaa acctgagacc tgaaacctga aacggtttct agctccagga
     4861 cagcgtcgcc aatccggccg gggcggcaat cgcatcaaaa tcgagaccag tggtcggcaa
     4921 gcgcaagccc aggtcttcgg ggtcgagcgg ccgcaagtcg ccgccagttg tctcgtttac
     4981 tatctggttg gcgcgggcct tgtcgaaccc ggcgccggtg actagaaatc tggaaagtgg
     5041 ttgttcaaat tcagagtcag ccacgcgacg gtcacggtag aagagcagca agcgatacag
     5101 ttcatcctca cgctcgtcgg gatcgcactc aatgctgcgg aagatcaggg gctgcttgtt
     5161 gcgatacacc accgccgtaa aaccttcgtt cgtcgaactg agcaacagcg agtcgccatt
     5221 gactccattg cgggttagcc attgagactc acccatgtga cgcggcaaca ccaaaccagc
     5281 gcgccagccc agcatgccaa agaggtgctc atactcagcg agcacggcga gacgggttgc
     5341 cacaaccaga taacgatcgc gtccctgaga atccttagcc aaacgctcac gcgagattga
     5401 cagctcgtcc aagggcacgc caaagccacg atccatcttc caccgcagca cttcttccaa
     5461 ttcgctaccg gatccgctgt ggggctcgag cgtaaggatc agggtgcgcg cagtcgcttc
     5521 agggagacta acggaccagc gcttttgttt tagcattcca gcactggccg cgagctctct
     5581 gatggctgcc gccaactccg agtgatcttc aatatttgat tcatcgaagc tcggcgaaat
     5641 caacgactcc ggtaacttca ccgttgccgc acgtcgcaga cttccagcat tgccacgccg
     5701 ttcaagctct acaactgagg ccagtccctt ctcgagtccg agagcagtcg ccggatacca
     5761 gggatggagc agtttggaaa gtggactggg cattacatat tttcgattgc cgattgccga
     5821 ttgccgattt aggactggcc aacagcaatg ctcataatct tcaccaacaa tcaacgtcaa
     5881 tctctaaacc ggaatgtccg gaaaccggaa cgcgttgttt tcttccaatt ggcaatcggc
     5941 aattggcaat cgccatcgca attacttcta ccattcagaa tacggcgttc cctcggacga
     6001 tgtgtccggg gaggcgctgc gaacgttgat cactcctgat tcaccggaag tcgagttggg
     6061 atcttcacca gtctcaacgg tccaatcctt ttctccagta atcggatcaa ccggtaactc
     6121 gcgcatgtag tgtccagcta ttagatcttc gagtgactgc ggcagttttc ctttatccgc
     6181 ggcgtactga tccagcagcg tgcgcatttt ataaaggtcg tcacgcaaca ccgcctcgcg
     6241 agcatgcaga attgtgcgct ggtactgcgg taaagcaatg gccgcaagaa tgatcacgat
     6301 tgtgatcaca atcatgagtt cgagaagggt gaagccaacc gcgagcatga gacagcggga
     6361 cgaggggata cgaagacgcg gggacgcggg gacgcgggga cgcggcgacg ttccaaacga
     6421 gcgtt
//
LOCUS       DASUEX010000027         2388 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_581185, whole genome shotgun sequence.
ACCESSION   DASUEX010000027 DASUEX010000000
VERSION     DASUEX010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2388
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_581185"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="VFV61_00485"
     CDS             complement(<1..412)
                     /locus_tag="VFV61_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4898973.1"
                     /translation="MRPIQSSTLIFALLISTFALQQRSSLSEQSPQSSRPNPQATPSP
                     TPTPSPSPSPVPLPPPTVKIDNYRAHAARIIGAALTSDKAYARLAHLTDHIGNRLSGS
                     KGLEQAIAWALAEMKRDGLDNVRGEKVMVPHWVRG"
     gene            complement(564..1400)
                     /locus_tag="VFV61_00490"
     CDS             complement(564..1400)
                     /locus_tag="VFV61_00490"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HEX4898974.1"
                     /translation="MIVHLVDGTYELFRHFYGLRRFKKTDPPFGAVSGVLRTVVQMLE
                     DEATHLGVATDHVIESFRNELWPGYKTSAGMERALLAQFHPLEEALLALGVAVWPMVD
                     LEADDALASAAHIAARSKRVDKVCIWTPDKDLSQCVVGDRVVQIDRRSNEIRNADRVR
                     AKFGVAPEFIADYLALVGDSADGYPGIPGCGAKTAASLINQHGHIEQFPAAVLKGDNR
                     VLALLFKDLATLRTNAPLFKSVEELRWKGPTPSFASVAKQIDDERLVARVEKLAMQLS
                     GK"
     gene            complement(1463..1822)
                     /locus_tag="VFV61_00495"
     CDS             complement(1463..1822)
                     /locus_tag="VFV61_00495"
                     /inference="COORDINATES: protein motif:HMM:NF018893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HEX4898975.1"
                     /translation="MQYVDGFVLPVPKKKLKLYRSIAQKAGKIWREHGALQYVEAVGE
                     DLEVKFGMSFPKAFKPKSGETIIFSWIVYKSRAHRDKVNAKVMSDPRLRKIMENSEMP
                     FDMKRMVYGGFQFLVNA"
     gene            complement(1935..2318)
                     /locus_tag="VFV61_00500"
     CDS             complement(1935..2318)
                     /locus_tag="VFV61_00500"
                     /inference="COORDINATES: protein motif:HMM:NF024953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX4898976.1"
                     /translation="MNIVLWIVQVLLALIFLFAGGTKLILSSEVLQSMGSPNQIMLPG
                     LLIKFIGLCEVLGGLGLLLPGLLRKRPNLTPLAAAGLLIIMIGAVVFTVAGDGVAAGI
                     APLIVGILCAFVAYGRSRLAPHVGS"
BASE COUNT          561 a          645 c          650 g          532 t
ORIGIN      
        1 gcccgcgcac ccagtgcggc accattactt tttcgcctct cacattgtcc agaccatcgc
       61 gcttcatctc ggcgagcgcc caggcgattg cctgttccaa acctttagac ccgctgagtc
      121 gattgccgat gtgatctgtc aggtgtgcca gtcgcgcgta agccttatca cttgtcagcg
      181 cagcgccaat aatgcgcgcc gcgtgcgctc ggtagttgtc gatttttact gtgggcggcg
      241 gtaacggtac tggcgacggt gagggcgacg gcgttggagt aggtgaaggc gtggcctgag
      301 gattcgggcg cgaggattgt ggagactgct cggacaaaga agatcgttgc tgcagggcga
      361 atgtcgaaat gagtaacgca aagatcagag tgcttgattg tattgggcgc acgattgacc
      421 tgttctccag atgaaattga aaagtattgc cgggagtatc cgaatcgttg ggaaaacctg
      481 ttggtaacgg ttgaagttta gccgatgact aacgacagcg gtagtggcag cggagcagcg
      541 tcggtgactg tacgggcttt gcgtcatttt ccggacaact gcattgccaa cttctcgact
      601 cgggccacga gccgctcgtc atcgatctgc ttcgctaccg atgcaaacga cggcgttggt
      661 cctttccagc gaagttcctc cacgctcttg aatagcgggg cattagtgcg gagggtcgcc
      721 agatccttaa agagcaaagc gagcacgcgg ttgtcgcctt tgaggacggc ggcgggaaac
      781 tgttcgatgt gtccgtgttg gtttatgaga ctggcggcag ttttagcgcc gcaaccggga
      841 atgccgggat aaccgtcggc tgaatcacct acgagcgcaa ggtagtcagc gataaactcg
      901 ggcgcaactc caaactttgc ccgcacgcga tcggcattgc gaatctcatt gctgcgtcga
      961 tcaatctgca ccacgcgatc gccaacgacg cattggctga ggtccttgtc gggggtccag
     1021 atgcagacct tgtctactcg cttgcttcgt gcggcgatat gggcagcaga ggcgagcgca
     1081 tcatcagctt caaggtcgac catgggccag accgccacgc ccagcgcgag caatgcttcc
     1141 tcaagtgggt gaaattgggc gaggagcgcg cgttccattc ccgcgctggt cttgtaaccc
     1201 ggccacagtt cgttacgaaa cgattcgatg acgtggtcag tggcgacccc aaggtgcgtt
     1261 gcctcgtctt caagcatttg cacgacggtc cgcaacacgc cactgaccgc cccaaacgga
     1321 ggatcagttt tcttgaagcg ccgaagtccg tagaagtgac gaaagagttc gtaggtgccg
     1381 tcaactaagt gaacgatcac agtcgaagtc caatgtccaa agtccaatgt ccgccagtcg
     1441 accttggacc ttggacttta gttcaggcat taacaagaaa ctgaaagccg ccgtagacca
     1501 tgcgtttcat gtcaaacggc atttcactgt tttccatgat ctttctcaag cgcgggtcgg
     1561 acatcacttt ggcattgacc ttgtcgcgat gagcgcgcga cttatagaca atccaggaaa
     1621 agatgatcgt ctcaccagac tttggtttga acgccttcgg aaacgacatg ccaaacttta
     1681 cttccagatc ctcaccgacg gcttctacgt actggagcgc accgtgttcg cgccagatct
     1741 ttcctgcttt ctgagcgatg ctgcgataga gttttaactt ctttttagga accggcaaaa
     1801 caaatccatc aacgtattgc atgacttctc ctttcgtgaa tcgtaaatcg tgaatcgaaa
     1861 gtcggaagtc ggaagtccgg aaagtgcagt tcggaagatt gcgccccccg attcacgatt
     1921 cacgattcac gatttcacga tcctacatgt ggtgccaaac gcgaacggcc gtaggccaca
     1981 aacgcgcaaa gaattcccac aatcagcgga gcaatcccgg ctgcgacacc gtcgcctgcg
     2041 acagtgaaaa caacggctcc gatcatgatg attaacagtc cggcagcagc aagcggcgtg
     2101 aggttgggcc gcttacgcaa gagtcccggc aaaagcaaac ccagcccgcc cagcacttcg
     2161 cagaggccaa tgaatttgat cagcaaaccc ggcaacatga tctgattggg tgagcccatc
     2221 gactgtagta cttcgctgga gagaatcaac ttcgttcccc cggcaaacag gaaaattagt
     2281 gccaacagaa cttgaacgat ccagagcaca atattcatga ttcattcctt aaacggcatc
     2341 tgccgtgatt caaccgcagc taactcttta acggaatcaa attttgca
//
LOCUS       DASUEX010000028        10430 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1497821, whole genome shotgun sequence.
ACCESSION   DASUEX010000028 DASUEX010000000
VERSION     DASUEX010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 10430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10430
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1497821"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            171..722
                     /locus_tag="VFV61_00505"
     CDS             171..722
                     /locus_tag="VFV61_00505"
                     /inference="COORDINATES: protein motif:HMM:NF019614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HEX4898977.1"
                     /translation="MPKYVAFLRAINVGGHVVKMDDLRALFEALGFTNVETFIASGNV
                     IFDSKSTNIGSLEKKIEKHLLKSLGYEVATFVRSVADLERIAGAKPFGAAELKSDFHA
                     LYVGFLAESPSEDARRELVKCGNKFNVFAFTEREVFWLCRVKFSETDFSGARLEKILK
                     MKTTLRNVNTPQRIAAKYSTSRG"
     gene            complement(723..1766)
                     /gene="waaC"
                     /locus_tag="VFV61_00510"
     CDS             complement(723..1766)
                     /gene="waaC"
                     /locus_tag="VFV61_00510"
                     /inference="COORDINATES: protein motif:HMM:TIGR02193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HEX4898978.1"
                     /translation="MRILIVRLSAMGDLVQTLPALTDAAKAIPRIKFDWVVDESFVQV
                     PSWHSHVENVFPTAMRRWGKNWVEAFQSGEPQALLKRLRATNYDLVIDLQGGFKSALA
                     SRLAKGPRAGHDSRGVHDWGAQIFYQKQFAVPKGMHSIQRMRRLLAAALGYPFPATAV
                     DYGIVRQKLVLPRLALPQPYVVFIHSTSWASKIWPERYWQELLKRAVAGGFYVVLPWG
                     DQAERLRSMAIRNGNERATVLPPLTISEKAAIIGEAQGTVGLDTGLSHIAAALDIPSV
                     TIYGATDPRLVGATGKHQVHLASQFECIRCHQTECTYPRPAEFKPACLVELKPDDVWE
                     KLLAAMLDGSSKT"
     gene            2077..2844
                     /locus_tag="VFV61_00515"
     CDS             2077..2844
                     /locus_tag="VFV61_00515"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX4898979.1"
                     /translation="MKITATIITLNEAEHIGLACESVSWADEILVVDSGSTDDTREIA
                     RAAGARVIENPWPGFAAQKQFAAEHAANDWIFSLDADERVSDQLRTSILTLRELDEKR
                     LVDGYSIARRAFYMGRWIRGGGWYPDRQLRLYKKTRGHWEGPYIHESVILDHEDHVAE
                     LSGDILHYPGSDAAEHHRLIGERYAPLGARQMFESGRRTSPPMIAFIGPATFARSFIL
                     KGGFRDGLAGLSIARFAAHHAFLKYLMLWELQNGSKQ"
     gene            2845..3405
                     /locus_tag="VFV61_00520"
     CDS             2845..3405
                     /locus_tag="VFV61_00520"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX4898980.1"
                     /translation="MDNSEFPINLRLATSQDIPALQTLIDQSVRALSVDYYTSAQIES
                     ALKNVFGVDTQLISDQTYFVAEAGGEIVGGGGWSKRQTLFGGDQAKSDETDTLLIPGE
                     QPARLRAFYVHPQWSRRGIGKLITRACESAARDAGFTSAELIATLPGQPLYEANGYEI
                     VGPFAIPLPDGLSLPAFRMAKNLIST"
     gene            3493..3978
                     /locus_tag="VFV61_00525"
     CDS             3493..3978
                     /locus_tag="VFV61_00525"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX4898981.1"
                     /translation="MKDFLDDFRQTIEDSAGRLLSLSAVSSQAAPAPGKWSPREVIGH
                     LIDSAAHNHVRFVRAQFTDDLQFHGYEQAEWVGVQGYNEESWEHLVQLWKLYNLHLLH
                     LISRIPAEQLTKQRHKHNLNELAWRAIPVEQPATLEYFIRDYLGHLKHHLRQISGIMT
                     D"
     gene            3991..4410
                     /locus_tag="VFV61_00530"
     CDS             3991..4410
                     /locus_tag="VFV61_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4898982.1"
                     /translation="MPYKPLRAFAYAVLIWVVGFIWGSIVFMTPALRSTAPIPYVSRN
                     PAISFPIILIWTVLTLLLARSYLKAAGEKERESLRLGLMFVIVNFVLDLVVLVFLLKT
                     GFSYFVSASVWFAYASLVLIPWLTGRTLASAPSNPTT"
     gene            4434..4754
                     /locus_tag="VFV61_00535"
     CDS             4434..4754
                     /locus_tag="VFV61_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4898983.1"
                     /translation="MAYTYEQLKHMTVAELREIAKGTDGEIVQGYTQLNKEHLVVALA
                     KALGIQHEHHDVVGVDKAAIKSRIRELKQKREAAIAAHDHAELKVVRRTIHRLKRQIH
                     KATV"
     gene            complement(4934..6544)
                     /locus_tag="VFV61_00540"
     CDS             complement(4934..6544)
                     /locus_tag="VFV61_00540"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEX4898984.1"
                     /translation="MTAKRNRLQLISLLVVAAAVISPWFSQRGAVTAEPQNRRSPIHI
                     TPPAPVFDQKARLSELAVRRSRVAQAIGPKGILILFSAEPRVYANDVDYEYRQENNLY
                     YLTMLKQNRATLVLLPGNAQLPEVLFLPRRNPLFEVWSGHMYTVDEAHAASGINEIWD
                     AREFEPFIQSLRKGQAYRPKPENIFKTKDAPAEAATSAFADLFAAAEKNQAELYMLPS
                     GGPNTREYRNEQRFVAEWEKTASGFVIKNAAPIFANLRLRKSELELRLLQHAIDITTE
                     AQERAWSVAGSANWEYEVDAQVAYTFKLRNADHWGYPSIVGCGPNATTLHYIESQGRV
                     EPGQLILMDVGAEYEHYTADVTRTFPVNGKFSPAQAEIYQIVFDAQEAGAKVIRPGAI
                     YSDVHRAATEVIKTGLLKLGLITDRNSEQYRLWFMHGTTHWLGMNVHDVGSTAKLEPG
                     MVFTNEPGIYVRLDALDNRPFNFRPGDWEKFKQEIRPAFEKYKGIGVRIEDDMLVTET
                     GTRWMTAALPRKLKDIEAFMARARQGSD"
     gene            complement(6605..8179)
                     /locus_tag="VFV61_00545"
     CDS             complement(6605..8179)
                     /locus_tag="VFV61_00545"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX4898985.1"
                     /translation="MLTPETVLQGRYRIVRSLGQGGMGTVYEAIDERLDSTVALKETL
                     FADERLRKQFEREARLLARVHHQALPRVSDHFSEGEGQFLIMQFIPGDDFAEKYAQRR
                     TPFPAADVMTWADQLLDALDYLHTQDPPIIHRDIKPQNLKVTSRGQVILLDFGLAKGQ
                     ASEISRVTTSASIFGYTPNYAPLEQIQGLGTDARSDLYALAATLYHLVTGVKPPDALT
                     RAASLVNGQPDPLQPAHEVNSAVPQVLSNVLTRAMAQNREQRFRNAAEMRRALQQGGL
                     ESTLTNRSEANTVLFPSAAPTPTLTDEAARATVGYLPATKPPTIPPTGETTLVAKPET
                     RRGPKWAMIAAALVLLVGGAGGYYAYQKRKAATVVALPTPSSAPSPAATPTPPENQVT
                     SGEGAAVNSVSVDTQKVEADKQQAANDAKQVRKEKPKQKVTTAKQTDTVEVDGDDGEE
                     FQPIRPPTPNVPNGDYSQIPNRQRLRRDGFTIRTLPDGSQLMTTADGTTILTKPDGTR
                     RIITPARRRRLLRPQP"
     gene            8430..>10430
                     /locus_tag="VFV61_00550"
     CDS             8430..>10430
                     /locus_tag="VFV61_00550"
                     /inference="COORDINATES: protein motif:HMM:NF015644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HEX4898986.1"
                     /translation="MSDQFPVSDAEIIDVAPARRRRWRRWLLGAAVLLFILLSRSLSI
                     YVSALWFGSMGYSPVYWYILKFKVVLFLIFLLLTAVILRTGFFLLERIFAVHALAPRT
                     VIVNNQPVQFAPARFLRPVSWGIALIFALFAGFSMKGKWLEFAAYLNRTQTSLSDPIF
                     QKPLEFYLFSLPVLDAISSWLVTLTFVLFCAALIYSLLSLPQTMLKNVRSLSTATSFA
                     ATSGALAAFLLAFAWRVYLSRFPYLWSDHQTFTGVTYTEAHYLLPALTLVALALVVAA
                     LIALLNAFTKRGLRLLLLAVAIPALVYIVGVVIIPAYVNSFIVKPNELGRETPYIEHN
                     ITWTRRAFGIDKIEQRNFEAETSVEAFALPDNRATLENIRLWDWRALQDTLKQMQAIR
                     IYYDFPDVDVDRYQTGGQTRQMMIAARELNIDKLPETSRNWVNQRLIYTHGYGVTMNT
                     ANEFTPEGRPRFVLSDMPIESISPDIPVKRPQIYFGQETNSNVYVKTKQHEFDFPQGE
                     TDTYTSYDGSGGIRIGSGLRRFLISWALGDLTKVPFSDAITSESRVLINRNIREIVNG
                     LAPFLIYDRDPYIVTGSDGNLYWIIDAFTETSNYPYSRHHNFVEDNVNYVRNSVKVVI
                     DAYHGTTNFYVFDPEDPVIAAYRATFPGLFRDASQMPADLRAH"
BASE COUNT         2282 a         2939 c         2775 g         2434 t
ORIGIN      
        1 acgagggtaa agtcggtggt tttcgcatta agctaatcaa actggatcct tatccgcaca
       61 ctgaacagaa gatctctccg ggcgactaca cggcgacgct attggtcaca agagaataat
      121 cctggcttct gcaccaagag ctagctactg tcgtttcgac ttcactgagt atgcccaaat
      181 acgtagcctt cctgcgcgcc atcaatgtag gtggccacgt cgttaagatg gacgatctcc
      241 gcgccctgtt cgaggcgctg ggttttacaa atgtggaaac gttcattgcc agtgggaacg
      301 tgatcttcga ctccaagtcc acgaacattg gatccttgga aaagaagatc gagaagcacc
      361 tgctgaagag tctgggttac gaagtagcaa cttttgtgcg ctcggtcgcc gatcttgaga
      421 gaatcgctgg ggccaaaccc ttcggcgccg cagagctcaa gtcagatttc cacgccctct
      481 acgtcggttt tcttgcggaa tcaccctcgg aggatgcgcg ccgagaactg gttaaatgcg
      541 gcaacaagtt caacgtcttt gcctttacgg aacgagaggt gttctggtta tgccgggtca
      601 aatttagcga gactgatttc tccggggcgc gactggagaa gatcttgaag atgaagacaa
      661 ccttgcggaa cgtaaacaca ccgcagcgca tcgccgcgaa gtattcgacg tcgcgcggct
      721 aatcaggtct ttgaactccc atccaacatt gcggccagca acttttccca cacatcgtca
      781 ggtttgagtt ccaccaggca cgccggctta aattcggcag gtcggggata ggtgcactca
      841 gtttggtggc agcgtatgca ttcgaattgg gaggccaggt gaacctgatg tttgccggtg
      901 gctccgacga ggcgcggatc agtggcgccg taaattgtga ccgagggaat gtcgagtgcg
      961 gcggcgatgt ggctcaagcc agtgtcgaga cccactgttc cctgcgcctc gccgataatc
     1021 gctgcttttt cggaaattgt gagaggcggt agcacggtcg cccgctcatt tccattcctg
     1081 attgccatcg agcgcagacg ttcggcttga tcaccccagg gcaagactac ataaaagccg
     1141 ccggcaactg ctcgcttgag tagttcctgc cagtaccgtt ccggccaaat cttcgaagcc
     1201 caactggtac tgtgaataaa gacgacgtaa ggctgcggca atgccaggcg cggcagaacg
     1261 agtttctggc gcactatccc gtagtcaaca gcggttgccg gaaatggata gcccagcgct
     1321 gcggccagta aacgcctcat gcgctgaatc gaatgcatgc ccttgggaac agcgaactgt
     1381 ttctgataaa aaatttgggc gccccaatcg tggacaccac ggctgtcgtg accggcgcgc
     1441 ggacccttgg ctaaccggct cgcaagcgcg ctcttaaaac caccctgaag gtcgatcaca
     1501 aggtcgtagt tcgtagcgcg cagccgtttc agtaacgcct gcggttcgcc gctctggaaa
     1561 gcctcgaccc aattctttcc ccagcgacgc attgccgtgg gaaagacatt ctcgacgtga
     1621 gagtgccagc tcggcacctg cacaaacgac tcgtcaacta cccaatcgaa ctttattcgc
     1681 ggaattgctt tggcggcatc cgtgagtgcg gggagcgtct gcacgagatc tcccatcgcg
     1741 gagagcctga ctattaagat acgcatggag tgtgactgct caggattgta tagtcacgaa
     1801 tcatcagtgt ctaaccagtc gaggtctttc aactaaccat caaggcgccc aggcgcagag
     1861 acgcaaagga cgacgaaaag gcagctaagc ctgttgatta ccctgacaca ggacgaagta
     1921 agcttttgag ctggcaacgt gttagctgcc tactcattct ttgcgtctct gcgccttggc
     1981 ggaaaactga aattccgggg ccgcaaacca cgttgacgcc tgcaacgccc gcttctattc
     2041 tctaatcggt ctgaaaccac tgtcacatta gcctgcttga aaatcaccgc caccattatc
     2101 actctcaacg aggccgaaca cattgggctc gcctgtgaat ccgtctcctg ggccgacgag
     2161 atcttggtgg tcgactcggg atcaaccgac gacactcgcg agattgcaag agcagctggg
     2221 gcgcgcgtca ttgaaaatcc gtggcctgga tttgccgccc aaaaacaatt tgctgcagag
     2281 cacgccgcaa acgattggat tttcagtctg gacgcggatg aacgcgtttc cgatcaacta
     2341 agaacttcga ttctcaccct gcgcgaactc gatgagaaac gcctcgttga tggctactcc
     2401 attgccagaa gggcgttcta catgggacgt tggattcgcg gcggaggctg gtatccagat
     2461 cgtcagttac gtctctacaa aaaaacaagg ggacattggg aagggcctta cattcatgaa
     2521 tcggtaattc tggatcatga ggatcacgtt gcagagttga gcggcgacat tcttcattat
     2581 cccggcagtg atgcggccga acatcaccgg ctgatcggcg aacgctatgc tcccctcggt
     2641 gcgcgccaga tgtttgaaag cgggcggcgc acttcgccac ccatgatcgc gttcattggc
     2701 cctgccacat tcgctcgcag ctttatcttg aaaggaggtt tccgcgacgg actcgccggt
     2761 ctcagcattg cccgctttgc cgctcaccac gcgtttctta agtatctaat gttgtgggaa
     2821 cttcagaacg gctcaaaaca gtaaatggac aactctgaat ttccgatcaa cctgcggctc
     2881 gcaacttctc aggacattcc ggcactacaa actctgatcg atcaatcagt acgagcgctc
     2941 agcgttgact actacacgtc ggcgcagatc gagagcgcac tcaaaaatgt cttcggggtg
     3001 gacacccaat tgatttcgga tcaaacctac tttgttgcgg aagctggcgg tgaaatagtt
     3061 ggtggcgggg gttggagcaa acgccagacg ctgtttggcg gcgaccaggc gaagtctgat
     3121 gaaacagaca cattgctgat tccaggcgag cagccggcgc ggctgcgcgc cttttacgta
     3181 catccgcaat ggtcgcgccg tggcatcggt aaactcatca cccgcgcctg cgagtctgcc
     3241 gcgcgcgacg ccggcttcac tagcgctgag ctgatagcga cactgcctgg ccagcctctt
     3301 tacgaggcca acggctatga gattgttggt cctttcgcaa taccgcttcc ggatgggttg
     3361 tcgctgccgg cttttcgcat ggcaaagaat ctgatatcca cgtaacgaaa gatagctacc
     3421 atcacgatac agtttcgcct gggaaatgag ccgcgctatt atctctgcgc accgtcgctt
     3481 cgaggccata ccatgaaaga ctttctcgac gactttcgtc agacgataga agactccgcc
     3541 ggccgcttgc tttcgctgtc ggccgtttcg agccaggcgg cgccggcgcc tggtaaatgg
     3601 tcgcctcgtg aagtaattgg ccatctgatt gactcagccg cccacaacca cgtgcgcttc
     3661 gtgcgagccc agtttactga cgacttgcag tttcacggtt atgagcaggc agagtgggtt
     3721 ggcgtccagg gttataacga ggaaagttgg gaacaccttg tccagctttg gaagctctac
     3781 aacctgcacc tgctgcacct gatcagccgg attcccgcag aacaactaac gaaacagcgg
     3841 cacaaacaca atctcaacga attagcctgg cgggccattc cggtcgagca accggccacg
     3901 ctcgagtatt tcattcgcga ctatctgggt catctgaagc atcatctgcg acagatctcc
     3961 ggcatcatga ctgactagaa tctgacgagc atgccttaca aacctctcag ggcatttgcc
     4021 tatgctgtgc tgatttgggt ggtcggcttc atctggggca gcatagtttt tatgacgccg
     4081 gcgctacgat cgacggcgcc gattccttat gtgtcccgta accctgcgat cagctttccg
     4141 atcatcttaa tctggacagt tttgacatta ctgctcgcgc gcagctatct gaaagcagct
     4201 ggagaaaagg agcgcgaaag tctccgccta ggcctgatgt tcgtaatcgt caacttcgtc
     4261 cttgaccttg tcgtgctggt atttctactg aagactgggt tcagctattt cgtctccgcg
     4321 agtgtctggt ttgcctatgc gagtctggtg ttaatcccct ggctcacggg gcgaacgctc
     4381 gcgagtgccc cttctaatcc gacaacctaa caagcagatt ggagtttcaa gcgatggctt
     4441 acacttatga acaactgaaa catatgacgg tcgctgagct gcgcgaaatc gccaaaggta
     4501 ccgacggtga aatcgtgcaa gggtacactc agctaaacaa ggaacactta gtagtcgcac
     4561 tggcgaaggc tctcggaatt caacacgagc atcacgatgt ggtgggagtg gacaaggcgg
     4621 caatcaagtc gcgaattcgc gagctgaagc aaaagcggga agccgcgatt gcggcccacg
     4681 accatgcgga gctaaaggtt gtgcggcgca caattcaccg tttgaagcgg cagattcaca
     4741 aagcaaccgt ctagaatatt gggtgattga atcattgaat cattgagtca ttgaatgatt
     4801 gggtcattgc cgattgccga ctgaaacttc ttcttactcg cacatacttg attcagtgat
     4861 ttcaatgagc caatgagcca atgactcaat gaccaaatga cccaatgatt caatggttca
     4921 atggttcaat cactcaatca ctcccttgac gcgcccgcgc catgaatgcc tcaatgtctt
     4981 tcagcttccg cggcagcgcc gcagtcatcc agcgcgttcc ggtttccgtc accagcatat
     5041 cgtcctcgat acgcacgcca atgcctttgt acttctcaaa cgccggccga atctcctgct
     5101 tgaacttttc ccaatccccg ggtctaaagt tgaaaggccg attgtccagg gcgtcgagtc
     5161 gcacatagat tcccggctcg tttgtaaaca ccatccccgg ctccagcttc gccgtggaac
     5221 caacgtcgtg cacattcatt cctaaccagt gagtagtgcc atgcatgaac caaagccggt
     5281 actgttccga attgcgatcg gtaattagac cgagcttgag gagtccggtc ttgattactt
     5341 ctgtagccgc acgatgcaca tctgaataaa tagcaccggg acgaatcacc ttcgccccag
     5401 cctcctgggc gtcaaaaaca atctggtaga tctcggcctg ggccggagag aacttgccat
     5461 tgacgggaaa ggtgcgcgtc acatctgccg tgtagtgttc atactccgca cccacgtcca
     5521 tcaaaatcag ttggccaggc tcaacccggc cctgcgattc gatgtaatgc aaggtggtgg
     5581 cattgggtcc gcagccaaca attgagggat aaccccagtg gtcggcgttg cgcagcttga
     5641 acgtgtaagc gacttgagcg tcgacctcat attcccaatt ggcgctcccg gcaacactcc
     5701 aggctctttc ctgcgcttcg gtagtgatgt caatcgcgtg ctgcaacaaa cgcaattcca
     5761 gctcagactt tcgcagtcgc agattcgcaa agattggcgc cgcgtttttg attacaaagc
     5821 cgcttgcagt cttttcccat tccgcaacaa agcgctgttc attgcgatat tcgcgcgtgt
     5881 tagggccgcc cgacggcagc atatagagtt cagcctgatt cttctctgcc gcggcaaaaa
     5941 gatctgcaaa agcggaagtg gcagcctccg ctggcgcgtc cttcgtcttg aagatgttct
     6001 ccggtttcgg tcggtaagct tgtcccttac gcaacgactg tatgaacggc tcaaactcgc
     6061 gcgcatccca gatttcatta attccagagg ccgcatgagc ttcgtctacg gtgtacatgt
     6121 gtcccgacca cacttcgaag agcggattac gtcgcggcag gaacagcact tccgggagct
     6181 gtgcattacc gggcagcagc acgagggtcg cgcggttctg tttcagcatc gtcaaataat
     6241 agaggttgtt ctcctgacga tattcgtagt ccacgtcatt ggcatagacg cgtggctcag
     6301 cgctgaacag gatcaggatc cctttcggac caatcgcctg tgccacacga gatcttcgca
     6361 ctgcgagttc ggaaagccgg gctttctgat caaataccgg cgccggtggc gtgatgtgaa
     6421 tcggcgagcg gcggttctgt ggctctgcgg tcaccgctcc tcgctgcgag aaccatggag
     6481 agataactgc cgcggctacg acaagtaatg agatcagttg caagcgattt cgttttgctg
     6541 tcatgggact ccggtacggg ccggtctctt tgatgtagag ctgagttaag gctggattgg
     6601 ggaatcaagg ttgcggccgc aggaggcggc gtcggcgtgc cggagtaatg atgcggcgag
     6661 tgccatcggg ctttgtaagg attgtggtgc catcagcagt tgtcatcagt tgactgccat
     6721 caggcagggt gcgaatggtg aaaccgtcac ggcgtagacg ctgccggttt ggtatctggg
     6781 agtaatcccc attcggaaca ttaggggtgg gcggacggat cggctgaaac tcctcgccat
     6841 cgtcaccgtc aacctcaaca gtgtctgttt gtttggctgt ggttaccttc tgcttcggct
     6901 tctctttcct cacctgcttg gcatcattcg ccgcctgctg cttatcagct tcgacttttt
     6961 gggtgtcaac agaaactgaa ttcactgccg ctccttcgcc actggtcact tggttctcag
     7021 gcggagtggg agtagctgcc ggcgaaggtg cgctgctggg cgtcggcaaa gcgacgaccg
     7081 tcgctgcctt ccgcttctga taagcgtaat aaccgccggc gcctcccact agaaggacaa
     7141 gcgccgcggc gatcattgcc catttgggac cgcgccgagt ttctggtttt gctaccagcg
     7201 tggtttcacc agttggcgga atcgtcggcg gctttgtagc ggggagataa ccgaccgttg
     7261 cccgcgctgc ttcgtcagtg agagtgggtg ttggcgcggc actgggaaat agaactgtgt
     7321 tcgcttcgct gcggttcgtc agagtgctct caagtccacc ttgttgcagc gcccggcgca
     7381 tctcggctgc attacggaag cgctgttcgc gattctgcgc catggcacga gtgagcacgt
     7441 tacttaagac ttgtggcaca gcgctgttaa cttcatgagc tggttgcagc ggatcaggtt
     7501 gtccatttac cagcgatgcc gcacgcgtaa gcgcgtcggg cggcttaacg cccgtcacca
     7561 ggtgatagag cgtggctgcg agtgcgtata gatcactgcg cgcatcagtg cccagtccct
     7621 ggatctgctc gagcggggca tagttgggag tgtaaccaaa gatacttgcc gaagtcgtga
     7681 cgcgcgaaat ctcactggcc tgccccttcg cgagtccgaa atccaggagg atgacttgcc
     7741 cgcgcgaggt cacttttaaa ttctgtggtt tgatatcgcg atgaatgatt ggcggatcct
     7801 gggtatgcag ataatccagc gcgtcgagca actgatccgc ccaggtcatc acatcggcag
     7861 ccggaaaagg agtgcgtcgt tgcgcatatt tctcggcaaa gtcatccccg gggatgaact
     7921 gcatgatcag aaactgccct tcaccttcgg aaaaatgatc actgacgcgt ggcagtgcct
     7981 gatgatggac gcgcgctaag agcctcgctt cacgctcgaa ttgcttccgc aaccgttcat
     8041 ccgcgaatag tgtctccttc aacgcaacag tactgtccag ccgctcatca atggcttcat
     8101 agacagtgcc cataccgccc tgccctaacg aacgaacaat acgatagcga ccttgtaaaa
     8161 ctgtttctgg cgtaagcatc aaaaaagttc tttaatcgat ggcgaccgat ggtcgaacaa
     8221 tggcctttga tgatcgaatt ggcggcgcgg ctgccagtct acttaactcg cggtcgcgca
     8281 cacatctcgc gcattataca cgccaaagtg ctgacatgaa ctgtgtcgca ctgcctcaag
     8341 cgcatcagcg cacggacaaa gcgaacatca gttaggacaa taagagcgat ccgtggggtc
     8401 gtttggccga attaaggtat gctaacagca tgagtgacca gttccccgtt tccgacgcgg
     8461 agattatcga tgtcgctccg gctcgccgtc gtcgttggcg acgctggcta ctgggcgcgg
     8521 ctgtcctgct gttcatcctc ctttcccgca gcctttcaat ctatgtgtcc gccctgtggt
     8581 ttggctcaat gggctattcc ccggtttatt ggtacatcct caaatttaag gtcgttctct
     8641 tcctgatctt tcttttgttg actgccgtaa ttctgcgcac gggatttttt cttctggaac
     8701 gaatcttcgc ggtccacgcg ctggcgccac gcactgttat tgttaacaac caaccggtac
     8761 agtttgctcc cgcccgcttc ttacgacctg tctcctgggg aatcgcactg atcttcgcac
     8821 ttttcgccgg cttttcaatg aaaggaaaat ggctggagtt tgctgcttat ctgaatcgca
     8881 cccagacctc actcagtgat ccgattttcc agaaacccct ggagttttac ctcttctctc
     8941 tgccggtgct cgatgcgatt agctcctggc tggtaaccct tactttcgtc ctcttttgcg
     9001 cggcgttgat ctactccctg ctttcgctgc cgcagacgat gctgaaaaat gtccgttcgc
     9061 tttctaccgc gaccagcttc gccgcgacat caggcgccct ggcggccttc ctactcgcgt
     9121 ttgcctggcg cgtttatctt tcgcggtttc cgtatctctg gagtgatcat cagaccttta
     9181 ctggcgttac ctataccgaa gcgcactacc tgcttcccgc gctaactctg gtcgccttgg
     9241 cgctcgtggt ggcagccttg attgcattgc tcaatgcctt tacgaaacgc ggactacgct
     9301 tgctgctgct cgcagtcgct attcctgccc tggtttacat agtcggcgtc gtgatcatac
     9361 cggcttacgt caacagcttt atcgtaaagc ccaacgagct ggggcgggaa acgccttaca
     9421 tcgaacacaa catcacctgg acccgccgcg cgttcggcat cgacaagatc gaacaaagaa
     9481 acttcgaggc cgaaacttcc gtcgaggcgt tcgcgctccc ggacaatcgc gccacgctcg
     9541 agaacattcg cttatgggat tggcgcgcgt tgcaggacac gctcaaacag atgcaggcga
     9601 tacgcattta ttacgatttt ccggacgtcg atgtcgatcg ataccaaacc ggcgggcaaa
     9661 ctcgccagat gatgatcgcg gcgcgcgaac tgaacatcga caaacttccg gaaacttcgc
     9721 gaaattgggt gaaccaacgc ctgatttaca cgcacggcta tggcgtcacc atgaatacgg
     9781 caaacgaatt cacgcccgaa ggccgcccgc gattcgtgct ctcggacatg ccgattgaat
     9841 caatctcccc ggacataccg gtcaaacggc cgcagattta tttcggacag gaaaccaaca
     9901 gcaacgttta cgtcaaaacc aaacagcacg aattcgattt cccccagggc gaaaccgaca
     9961 cctacacgag ctacgacggt tctggcggca ttcgcatcgg cagcggcttg cgccgcttcc
    10021 tcatctcctg ggcgcttggc gatttgacga aggttccctt ctcagatgcg atcacttcag
    10081 aaagccgggt actcatcaat cggaatatta gagagatcgt caacggtctg gcgccctttc
    10141 tgatctatga ccgcgaccct tacatcgtca ccggcagcga cggcaacttg tattggataa
    10201 tcgatgcgtt taccgagacg tcgaattatc cgtactctcg acatcacaat tttgtcgaag
    10261 acaacgtcaa ctacgttcgc aacagcgtaa aagtcgtgat cgatgcgtat cacggcacca
    10321 ccaatttcta cgttttcgat cctgaggacc cggtgatcgc ggcctaccga gcgaccttcc
    10381 ccggcctttt ccgagacgcc agtcagatgc ccgcggacct gcgcgcccac
//
LOCUS       DASUEX010000029         2804 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1266066, whole genome shotgun sequence.
ACCESSION   DASUEX010000029 DASUEX010000000
VERSION     DASUEX010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1266066"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            436..511
                     /locus_tag="VFV61_00555"
     tRNA            436..511
                     /locus_tag="VFV61_00555"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:469..471,aa:Phe,seq:gaa)
     gene            complement(542..1873)
                     /locus_tag="VFV61_00560"
     CDS             complement(542..1873)
                     /locus_tag="VFV61_00560"
                     /inference="COORDINATES: protein motif:HMM:NF005557.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxyadenosine deaminase"
                     /protein_id="HEX4898987.1"
                     /translation="MTESILIKGGTLITMDEQDSIVQGNIFIAGGRIQNIGDSTPVAD
                     VVIAADGCAILPGFVQTHIHLCQTLFRGAADDLALIDWLKLRVWPMEAAHSAESVRAS
                     AQLGVAELIKGGTTAALTMETVNHTDEVFRVVEESGFRATVGKCMMDKGDDLPPQLHE
                     RTRDSIEQSIDLLESWHGKAEGRIRYCFAPRFAVSCTGELLSEVARLARERGVMIHTH
                     ASENRKECEIVESETGMRNIAYLDSLGVSGSHVALAHCVHVDDAEIALLAGTQTNVSH
                     CPSSNLKLGSGIAPVKKMLEQRVRVSLGADGAACNNRLDMFTEMRTAALLQKVMHGPE
                     VIPARQALRMATIEGARTLGLGAEIGSLEVGKRADVIVVGLESLHSMPSAFDPVSAIV
                     YSSEPSDVRAVIIDGRVVMRDQKLFTLNEQEITEKSGREAELLLKRAGISR"
     gene            complement(1991..>2804)
                     /gene="recA"
                     /locus_tag="VFV61_00565"
     CDS             complement(1991..>2804)
                     /gene="recA"
                     /locus_tag="VFV61_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEX4898988.1"
                     /translation="VAEAQRAGGVAAYIDAEHAMDADYAGKLGVDIDQLLISQPDSGE
                     QALEIAEALVRSNGVDVIVVDSVAALVPRAELDGEMGDSLPGLQARLMSQALRKLTAI
                     VSQSNTCFIFINQIREKIGVMFGNPETTTGGRALKFYSSLRLDIRRIGAIKDGDRVVG
                     NRTKVKVAKNKVAPPFRECEFDIMYGEGISREGDVLDLGATHRVVEKSGAWFSYKGER
                     LGQGRENAKQALKDNPEILKRIENEVKAKLGMAVKEEKEPAANAKAAAAEKK"
BASE COUNT          619 a          817 c          717 g          651 t
ORIGIN      
        1 cgagtcggag acggtggcga atgctagtgc ggaagctaag aagctggtgg agcgggcttg
       61 aaattgggcc tgaaaatagc tgtgccagct cgaaggtagc gactgcgggg ggcgcgatcg
      121 gcggccgagc tggcactggc gggcactatc aattttgaat tggatggctg tcttttcgac
      181 ctagctgacg tttgggcatc agctccaccc gcaacgtttt gaattataca cccggcaaga
      241 aatcagtcaa cgactttttg tgacgaatcc gacaaaatcc tcaaagccgt atgaacaacc
      301 gttccagaaa cctatgtgct gttaatagat acggccgcat cacgctctcg atacgactaa
      361 atgattcctt acaaggccgg caagttgaca gttcttttag gaacttccta tactcgcggt
      421 ctttccaaaa tgactggcca ggtagctcag ttggtagagc gcggccctga aaaggccggc
      481 gtcggcggtt cgattccgtc cctggccacc atttaatccg tcaaaattcc cgatcctact
      541 ttcaccgact gatgcccgct cgcttgagca gtaactcagc ctctcgacct gatttctcag
      601 taatctcctg ttcgttcagg gtgaacagtt tttggtcacg catgaccact cttccgtcga
      661 taattaccgc tctgacatcc gagggttcgg acgagtaaac gatggcagag acgggatcaa
      721 atgcgctggg catggagtga agcgattcaa gccccacaac aatcacatcg gcccgcttac
      781 caacttccaa actaccaatc tccgcaccca acccaagtgt cctcgcacct tcgatcgttg
      841 ccattcgcag agcttgtcgc gccggaataa cttcaggacc atgcattact ttttgtagta
      901 aggcggccgt gcgcatctcg gtaaacatgt cgagccggtt gttgcacgct gcgccgtcag
      961 cgccaagcga aacccgcacc cgttgctcca gcatcttctt cactggggca atgcctgagc
     1021 ccagcttcaa attcgaagaa ggacaatggc tgacgttggt ttgagttccg gccagcaatg
     1081 caatttcggc gtcatccacg tgaacgcaat gagccagggc gacgtgtgaa ccgctcacgc
     1141 caagcgaatc caggtatgca atgttgcgca tacccgtttc gctctcgacg atctcgcatt
     1201 ctttgcggtt ctcagaggca tgagtgtgaa tcatcacgcc tctttcgcga gccagacggg
     1261 ctacttccga caaaagctcg cccgtgcagc taaccgcaaa gcgtggggcg aaacaataac
     1321 ggattcggcc ttcagccttg ccgtgccacg actcaaggag atcgattgac tgctcgatcg
     1381 aatctcgcgt gcgctcgtgc aactgcggcg gcaagtcgtc ccccttgtcc atcatgcatt
     1441 tgcctacggt ggcgcgaaag ccgctctcct caaccactcg gaatacctca tccgtgtgat
     1501 tgaccgtctc catcgttagc gcggcggtgg tcccgccctt gatcaactct gcgacgccga
     1561 gctgggccga ggcgcgcact gactcggcgg agtgggccgc ttccatcggc cagacacgca
     1621 atttcaacca gtcaatcaga gccagatcat ctgctgcacc gcgaaacaat gtctggcaaa
     1681 ggtgaatgtg ggtttgcaca aagccaggta ggatcgcgca accgtcggcg gcgatgacaa
     1741 catccgccac gggcgtggag tcgccgatat tctggatccg cccgccggca atgaagatgt
     1801 ttccctggac gatggagtct tgctcatcca tcgtgatcag ggtgccacct ttgatgagta
     1861 ttgattctgt catgccgcgg cggattatcg gttcagattc aaggtcgagt aaagcaaaag
     1921 aagcgctcaa acaggaaatc actttggagc gatctcagtc tgggcgcttc ttgcagctct
     1981 cgagcgaacc ttatttcttc tcggcagccg cggcctttgc gttcgccgcc ggctccttct
     2041 cttcctttac cgccattccc aattttgctt tcacctcatt ctcaattcgc ttgagtatct
     2101 ccggattgtc tttcaaggcc tgcttggcat tttcacgtcc ctgcccaagt cgttcgccct
     2161 tgtaagaaaa ccaggcccca cttttttcga cgacccggtg cgtcgcgccg aggtcgagca
     2221 catctccctc gcgcgagatg ccttcgccgt acatgatgtc aaactcgcac tcacgaaatg
     2281 gtggcgcaac cttattcttt gcgaccttaa cctttgtgcg attacctacc acccggtcac
     2341 catccttgat tgcgccaatg cggcgaatat cgagtcgcaa cgaagaatag aatttcaggg
     2401 cacggccccc ggtagtggtt tcggggttgc caaacatcac gccaatcttt tctcgaatct
     2461 gattgatgaa gataaagcag gtgtttgact gtgaaacgat agcggtgagt tttctcaggg
     2521 cttgcgacat caaacgggcc tgcaagcctg gtagtgaatc acccatctcg ccatcgagtt
     2581 cggctcttgg gacgagggcc gcgactgagt caaccacaat cacatcgacg ccatttgaac
     2641 gcacgagagc ttctgcaatc tcgagggcct gctctcctga atcaggttgc gaaatcagga
     2701 gctgatcgat atcaacaccc agcttaccgg cataatcggc gtccatggca tgttcggcat
     2761 caatataggc agccacgcca ccagcccttt gtgcttcagc aacg
//
LOCUS       DASUEX010000030         2920 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_581937, whole genome shotgun sequence.
ACCESSION   DASUEX010000030 DASUEX010000000
VERSION     DASUEX010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2920
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_581937"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..670
                     /locus_tag="VFV61_00570"
     CDS             <1..670
                     /locus_tag="VFV61_00570"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HEX4898989.1"
                     /translation="AVIGDQTTVGAGTRIEAGVVIGDNVTIGVDCVFHPNVSLYDSVT
                     LGDRVVLHAGVCIGADGFGYVRDDMGYQKFPQIGTVVIEDDVELGAHTCVDRGALGRT
                     RIGRGTKIDNLVQVGHNVDIGERVVIASQTGISGSVIIEDDCVIGGQVGFGDHIRVQS
                     GAVIGSKAGILPGKIVRPGVWWGVPIIPLAEYKIRNAHLGRLPELREELSDLRRRLEE
                     LERK"
     gene            complement(730..1920)
                     /gene="lpxB"
                     /locus_tag="VFV61_00575"
     CDS             complement(730..1920)
                     /gene="lpxB"
                     /locus_tag="VFV61_00575"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HEX4898990.1"
                     /translation="MNSDPRRLMIVTGEPSGDAHGAALVQALRAAATGPLEIFGATGP
                     LLRAAGVESVIHSDDLSIMGIVEVGRVLPKFWNAYKALKRAALERKPEAVILVDWPEF
                     NFRLARVFHRRGLKVIYYISPQLWAWRSYRLRTIKRDVDLLLAILPFEKEWYAERGVD
                     HVEFVGHPLAGEVRAIYDRTEFCRRAKLDPKLPIISLLPGSRQKELERILPPMLEAAA
                     LISARRPEIQFVVVVAPSRSSDEIREIARTARATPLPLPSCLHIATGSTREALAASDV
                     AAVASGTATLEAALLGTPMVIVYKESAINWHTLGRLITTEHFGLVNLIAGEEVATELM
                     QDELNGESLANELLLLLEPERNRVARERLSEIAEQLGEGGASARAAKRILEFLKPHEA
                     GRSL"
     gene            2340..>2920
                     /locus_tag="VFV61_00580"
     CDS             2340..>2920
                     /locus_tag="VFV61_00580"
                     /inference="COORDINATES: protein motif:HMM:NF015901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HEX4898991.1"
                     /translation="MRAATRRIIFQFVLITLSGILLSAAAPAVAQQTNPVDRKVTNPM
                     TDTPNVNPLVQDQPVRPRLPPRRGAQGETTEELKIDASTQTVSGPPNAQVFVYEGNVD
                     ARIGTYRLQADKVTVYEATNKVVAEGNVVFDQSDQQRITGSRAEWNYRTKTGFFIDST
                     GFTNQTEDGTRIYFTADRVEKISLDTIVATNAQV"
BASE COUNT          666 a          817 c          806 g          631 t
ORIGIN      
        1 tgcagtaatc ggcgaccaaa ccaccgtggg cgccggcacc agaatcgagg cgggggtggt
       61 aattggcgac aacgtcacta tcggcgtgga ctgcgtattt catcccaatg ttagtctcta
      121 tgacagcgtg acgctgggtg accgagttgt attgcatgca ggggtctgca ttggcgccga
      181 tggctttggt tacgtgcgcg acgacatggg ctatcaaaaa tttccgcaaa tcggcacggt
      241 ggtgattgag gacgatgtag aactgggagc gcacacttgc gttgatcgcg gagcgctcgg
      301 tcgcacgcga attggacgtg gaacaaagat cgacaacctg gtgcaagtag gtcacaacgt
      361 cgatatcggc gaacgagttg tgatcgcctc gcaaacggga atttccggca gcgtgattat
      421 cgaagacgat tgcgtgattg gcggtcaggt gggttttggc gatcacattc gggtgcaaag
      481 cggcgcggta attggctcca aggcggggat cttgccaggg aagatcgtcc gaccgggagt
      541 ctggtggggg gttccgatca ttccgctcgc tgaatacaag atccgcaatg cgcatcttgg
      601 ccgcttacct gagttgcgcg aggagcttag tgatctcagg cgccggttgg aagaactgga
      661 gcggaagtga agacccaacc tacggcttgc cgcacggagg acggcagagc cgttaccgct
      721 gctgtcgcac tacaggctgc gccctgcctc gtggggtttt aggaactcca aaatcctttt
      781 tgccgcccgc gccgacgcac cgccttcccc cagttgttct gctatctcac tcaacctttc
      841 ccgtgcaacg cgatttcttt ccggctcgag caacaagagc aattcgttag ctaaactctc
      901 gccgttcagc tcgtcctgca tcaactcagt cgcaacttct tcgcccgcaa tcaggttcac
      961 cagaccaaaa tgttcggtcg ttatcagacg gcccaatgta tgccagttaa tggccgactc
     1021 tttgtagaca attaccatcg gtgttcctaa gagcgcggct tcgagcgtgg ccgttccact
     1081 tgctaccgcc gcgacgtcgg aagcggcgag ggcttcgcgc gttgagccag tcgcgatatg
     1141 cagacacgaa ggcaacggta acggtgtggc gcgcgctgtc cgcgcgattt cccgaatttc
     1201 gtcggaggaa cggctcggcg cgaccaccac gacgaattga atttcgggcc ggcgcgcgga
     1261 gatgagggct gcagcttcca acatgggcgg cagaatgcgt tccagttctt tctgccggct
     1321 accgggaagc agagaaataa tcggcagttt gggatccagt ttggctcgcc ggcagaattc
     1381 tgtgcggtca tatatcgcac gaacttcgcc ggcgagcgga tgaccaacga attccacatg
     1441 atcaacgcca cgctcggcgt accactcttt ttcaaagggc aggatagcca gcagtaaatc
     1501 aacgtcacgc ttgatggttc gtagtcgata tgagcgccag gcccagagct gaggactgat
     1561 gtagtaaatg actttcaggc cgcgccggtg gaacacccgc gcgagtcgaa aattgaactc
     1621 aggccaatca acgaggatga ctgcctccgg ctttcgttca agtgcagcgc gtttcagcgc
     1681 cttgtatgca ttccagaact ttggcaagac ccggccgact tcgacgattc ccataattga
     1741 cagatcatcc gaatggatta ctgactcaac gccggccgcg cgaagcaagg ggccagtggc
     1801 gccgaagatc tcgagcggcc cagtcgccgc ggcgcgtagc gcctgcacta aagccgcgcc
     1861 atgtgcatca ccggaaggtt cgccggtcac gatcattaat cgtcgggggt cggaattcat
     1921 cagtcgcagt agacagtaga ccggagacgg aaggcaagac ggcggaaggc cgtgaaaagc
     1981 aaaacccatt ttccatcatt cttcatttct catttttcat tcggttctca cgcttaaggg
     2041 ttaccaagcg gcaatcgcca atcggcaata catgaataat catcattaga gctcttgacc
     2101 tagttacgaa ttgaacgtga gatccaaacg aaaaatgaga aatgagaaat aacatatgga
     2161 aaatggtttt cctgctcctg ccgctgcctc ctgctccgct ctggcctctg atttccgtct
     2221 cctgcctcgt gcttttgtgt tagcatcgcc tcgctctaca taaacaaggc gcgcgctttt
     2281 tcgcgggatt atttttggtt agtgctcgcc cgttgaattc gggaggtctt cacactcgtt
     2341 tgcgcgccgc gacccgcaga atcatcttcc aattcgtact aatcaccctc agcggaattc
     2401 tactgagtgc cgctgcgcct gcagtcgcac aacaaaccaa tccggtcgat cggaaagtta
     2461 cgaatccgat gaccgacact cccaacgtca atcctctcgt acaggatcaa cctgtccgtc
     2521 cgcggttgcc tccacgacgc ggcgcgcaag gcgaaaccac tgaagagctg aagatcgacg
     2581 ccagcacaca aactgtttcc ggcccgccta atgcacaggt gttcgtctac gaaggtaacg
     2641 tagatgcgcg catcggcact tatcggctgc aagctgacaa ggtcaccgtt tacgaagcca
     2701 caaacaaggt ggtcgccgaa ggcaatgtgg tttttgatca gagcgatcag caacgcatca
     2761 ccggttctcg tgctgagtgg aactaccgta ccaagaccgg cttctttata gactcaaccg
     2821 gcttcacaaa tcagactgaa gacggcacac gcatctactt caccgccgat cgggttgaga
     2881 aaatcagcct cgacaccata gtggcaacta acgcccaggt
//
LOCUS       DASUEX010000031         2500 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_468574, whole genome shotgun sequence.
ACCESSION   DASUEX010000031 DASUEX010000000
VERSION     DASUEX010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468574"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(182..331)
                     /locus_tag="VFV61_00585"
     CDS             complement(182..331)
                     /locus_tag="VFV61_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4898992.1"
                     /translation="MRVRKVVRSLEGGLAPAFLFRQQLLAGSKRYPIDSEVEIVPLRS
                     GIGKM"
     gene            364..1137
                     /locus_tag="VFV61_00590"
     CDS             364..1137
                     /locus_tag="VFV61_00590"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX4898993.1"
                     /translation="MSTEPNKVIYSMVGVSKFYDKKPVLKDIYLSYFYGAKIGVIGLN
                     GSGKSSLLRILAGVDKDFNGETVISPGFNVGFLEQEPLIDDSRTVREVVEEAVQETVD
                     LLKEFEEINAKFGEEMSDDEMNSLLERQGVVQEKLDQLDAWDLDSRLEMAMDALRCPP
                     PETPVKILSGGERRRVALCRLLLQKPDILLLDEPTNHLDAESVAWLEHHLQSYPGTII
                     AVTHDRYFLDNVAGWILELDRGAGIPWKGNYTSWLEQKQ"
     gene            1183..2046
                     /locus_tag="VFV61_00595"
     CDS             1183..2046
                     /locus_tag="VFV61_00595"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX4898994.1"
                     /translation="MQRELEWIRMSPKARHAKGKARINSYEAMLREESEKRREDLEIH
                     IPPGPRLGNVVIEADKVSKGYGANLLVEDMTFALPPGGIVGVIGPNGAGKTTLFRMIT
                     DQEKPDSGTIRIGETVVLAYVDQSRTLDPNKSIWEEISGGTDLLKLGNREVNSRAYVG
                     RFNFSGSDQQKKVGMLSGGERNRVHLAKMLKESANVLLLDEPTNDLDVNTLRALEEAL
                     ENFAGCAVVISHDRWFLDRIATHMLAFEGDSKVVWFEGNYSEYETDRKERLGAAAEQP
                     HRIKYRQLTRG"
     gene            2138..>2500
                     /locus_tag="VFV61_00600"
     CDS             2138..>2500
                     /locus_tag="VFV61_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4898995.1"
                     /translation="MIAQHNVATPYADRRFFTAMSILAAITVFAGFAPTYYLKGAFGA
                     PALPLLVHFHGLLFTSWILLFIIQTSLVAVRRTDVHRRLGILGGLLASGMVLVGFLTA
                     IAAARRGVSPPGSPPALQF"
BASE COUNT          580 a          664 c          692 g          564 t
ORIGIN      
        1 gggccacgat cgcaaaaagt tacagagcta ttcaactgac tttttcaaga gcgtgttggg
       61 aaggtagtga atcgcagcat cgcggacgtt ccgccatcca tacgctcagt ccggcaggtg
      121 ggcttgcccc gcaatgagcg cgcagtgcga acagccgggg ctcggttacc ttcttccgct
      181 ttcacatctt gccaatccca cttctcaacg gcacaatctc aacttcggaa tcgatcggat
      241 atctcttcga tccggcaagg agttgctgac gaaacaagaa agcgggggca agcccgcctt
      301 ccagactgcg cactaccttc cggactcgca ttgcatgtcg gcattattga aacgattgga
      361 agtatgagca ctgaaccaaa caaggttatc tattcgatgg tgggcgtcag caagttctac
      421 gacaaaaagc ccgtcctgaa agacatctat ctctcctact tctacggcgc aaagattggt
      481 gtcatcggcc ttaacgggtc cggcaaatcg tcactcctgc gcatcctcgc gggcgtagac
      541 aaagacttca acggcgaaac cgtgatctca ccggggttca atgtcggctt tctggagcag
      601 gaacccttaa ttgacgactc gcgcactgtt cgcgaggtgg ttgaggaagc ggttcaggaa
      661 actgttgact tgcttaagga gtttgaagag atcaatgcga agtttggtga ggagatgtcc
      721 gacgatgaga tgaattcact tctggaacgt caaggcgtag tccaggagaa gctggatcaa
      781 ctcgacgcct gggatcttga ctcacgtctg gagatggcca tggacgcgct ccgctgtccg
      841 ccgcccgaaa ctccggtgaa gattttatcc ggcggtgaac gccggcgcgt ggcgctttgt
      901 cggttgctcc tacagaaacc cgatattctg ttgctggacg aacccacaaa tcatctcgac
      961 gccgaatctg ttgcctggct cgaacatcac ctgcagagct atccgggaac aatcatcgcc
     1021 gttactcacg atcggtattt tctcgacaac gttgccggct ggatcctcga actcgatcgg
     1081 ggcgcgggca ttccctggaa aggcaattac acttcgtggc tggagcagaa gcagtagcga
     1141 ctgcgacgcg aggaaaagtc cgagagcgag cggcagaaga ctttgcaacg cgagcttgaa
     1201 tggatccgca tgtcacccaa ggcgcgacat gccaaaggca aggcacgcat taattcctac
     1261 gaagcgatgt tgcgagaaga gtccgaaaag cgccgcgaag atctggagat tcacattccg
     1321 cctggcccgc ggctgggcaa cgtcgtgatt gaagccgata aagtcagcaa aggttacggc
     1381 gccaatttac tggttgagga catgactttt gccctgccgc caggcggaat tgtcggggtg
     1441 attggaccaa acggcgctgg taagacaact ctgttccgga tgatcacaga tcaggaaaaa
     1501 cctgatagcg gcacgattcg gatcggcgaa accgtagtgc ttgcttatgt cgatcagagt
     1561 cgaactctcg atcctaataa gtccatttgg gaggaaatct ccggcggcac tgaccttttg
     1621 aaacttggca accgcgaggt aaactctcgt gcctacgtcg ggagattcaa cttctctggt
     1681 tcggatcaac aaaagaaagt cggcatgttg tccggcggcg aacgcaatcg cgttcacctc
     1741 gccaagatgt tgaaggaaag tgcgaatgtt ctgctgcttg acgaaccgac gaatgatctc
     1801 gacgtgaaca cactgcgagc cctggaagaa gctctggaaa actttgctgg ttgcgcagtg
     1861 gttatttctc acgatcgctg gtttctcgat cgcatcgcga cacacatgct ggcatttgaa
     1921 ggcgacagca aggttgtttg gttcgaaggt aactattccg agtatgaaac tgatcgtaaa
     1981 gaaaggctcg gagcggcagc tgagcagccc catcgtatta aatataggca attgactcgt
     2041 ggatagtgtc gataaggagg ttagagtccc cttcggggat caacccacgg ctattcggtt
     2101 cgcttcgagg accaaaaaag gagtaggagc ttcccgcatg attgctcaac acaacgtcgc
     2161 aaccccctac gccgaccggc gctttttcac tgccatgtcg atccttgcgg cgatcacagt
     2221 tttcgcaggc ttcgcgccaa cctattatct taagggcgcg ttcggcgcac cggcgctgcc
     2281 gctcctggtt cacttccatg gcttgctctt cacgtcctgg attctgctct tcattattca
     2341 aacatcactc gtggcggtac gacgcactga tgttcatcga cgactgggaa tcctgggtgg
     2401 cctgctggcg agtggaatgg tgctggtcgg ttttctgacg gctatcgctg ctgcccgtcg
     2461 cggcgtgagc ccgccaggta gtcctcccgc attgcagttc
//
LOCUS       DASUEX010000032         5542 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_926887, whole genome shotgun sequence.
ACCESSION   DASUEX010000032 DASUEX010000000
VERSION     DASUEX010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5542
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_926887"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            224..1336
                     /locus_tag="VFV61_00605"
     CDS             224..1336
                     /locus_tag="VFV61_00605"
                     /inference="COORDINATES: protein motif:HMM:NF014047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HEX4898996.1"
                     /translation="MHGDVIIIRLIFTAILIVAGYVLRPIAGDRWISAGVGALIAICI
                     ILFELRIRRATLKTLIGAAVGSIMGIVGAFLIGSLISRQESMAVAPEVKTFVTLALTF
                     FMAYVGLMVGAAKGDYLELSALGGILSEKASRRDLKILDTSVIIDGRIADVAETGFLT
                     GSLIIPQFILRELQQVADSPDSSKRQRGRRGLDMLHRLQNNSSLDIQIVETDFPSVRE
                     VDLKLIELGKQLDAVIVTNDFNLNKVSQLRGVSVLNINELANALKPVVLPGEAMRVFI
                     LKEGKEYNQGVAYLDDGTMVVVDNARKLIGKNADIAVTSVLQTTAGKMIFGRLWEDAE
                     NGNGEHRTNGSARRQPRDSRSTPGPVRITDTEPVID"
     gene            1446..2123
                     /gene="ispD"
                     /locus_tag="VFV61_00610"
     CDS             1446..2123
                     /gene="ispD"
                     /locus_tag="VFV61_00610"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEX4898997.1"
                     /translation="MNVAIIAAAGQGTRMGGKRAKQFLVLAGRPIIIHTIQAFEACEA
                     IQEIIVVLPEADVRDFSAVARPYNLGKVRAVVPGGVARASSVLHGLRAVNAQETNIVA
                     VHDGSRPLVTVAEITRTVEAAEVSGAAILVAPIVDTVKEVREGTVVATVPRDHLRRAL
                     TPQCFRYEILRQAYEQVEEVDATHTDESSLVERLGVTVSVVEGSSRNIKVTGPEDLVI
                     AEALLKT"
     gene            2216..2698
                     /gene="ispF"
                     /locus_tag="VFV61_00615"
     CDS             2216..2698
                     /gene="ispF"
                     /locus_tag="VFV61_00615"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF014588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEX4898998.1"
                     /translation="MFRIGIGNDTHRLVEGRPLIIGGVQLAATRGAEGHSDADALAHA
                     IADAILGAICEGDLGVHFPDRDEQWKDADSLQLLSRVMWLANERGLRVVNLDATVQLE
                     EPKLRPYMLSIRENIAATLGVELGCVSVKAKTGEGLDAVGQGLAITAQAAVLMTSLAS
                     "
     gene            2722..4044
                     /locus_tag="VFV61_00620"
     CDS             2722..4044
                     /locus_tag="VFV61_00620"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF004490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HEX4898999.1"
                     /translation="MRPQDVIRKKRDGEELSRAEIDFFIGGITNDGIADYQVSALLMA
                     IFLNGMNEAEQQALTEAMLRSGITLDFSGIAKPKADKHSTGGVGDKTSLIIAPLVAAC
                     GVCVPMISGRGLGHTGGTLDKLESIPGYRVNLSTKEFREVLEQVGFAMAGQTAELAPA
                     DKKMYALRDATSTVEAIPLIVASIISKKGAAGLDAMVIDVKVGSGAFMREEARARALA
                     KALVKTGNSCGIRTRALLTDMNQPLGRAVGNSLEVKECIELLRGEAEPGARPVLDLSL
                     ELSAHMLVLARVDDSLEAAHARLQRVLDAGKALECFRQNIAAQGGDARVCDDPASFLP
                     LVSDSIKVESPRSGFITAIETTEIGHAIAALGGGRVRIDDKIDPTVGFIAEKKIGDKV
                     EKGELLGTVYARDQARARETAARIQASYCVSEASHSQQPTLIKEVINE"
     gene            4041..5135
                     /locus_tag="VFV61_00625"
     CDS             4041..5135
                     /locus_tag="VFV61_00625"
                     /inference="COORDINATES: protein motif:HMM:NF014647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX4899000.1"
                     /translation="MKRSTWSLYLLAGLILLSLIASSCSNQSYGSEDTSKIRVGIVFD
                     IGGKDDRSFNAAAWAGAKCAATGNWPDGTSCGKQRLDIVLRDIEPGNPTSIEPAMRAF
                     AERNYDLIIGIGFAQTPIIESVAKDYPNIRFAVVDGVSNLPNVASLLFKEHEGSYLVG
                     MMAARTTQTGTIGFLGGMDIGLIHRFKEGYEEGAKAVNPNIRVIPNYVGVTDAAWNNP
                     GKGKEIALAQISKGADVIFTAAGNSGLGAFDAVEQQGKKNGRATHFVIGVDANQNMVK
                     PGFVLTSMVKRVDNAVYDIIKDVVSGQFKTGFHVYGLDKDGVGYAMDENNAMDDNKKP
                     LISPDAIQQVEEARKKIIAGEIKVTDAMNK"
     gene            5223..>5542
                     /locus_tag="VFV61_00630"
     CDS             5223..>5542
                     /locus_tag="VFV61_00630"
                     /inference="COORDINATES: protein motif:HMM:NF017451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEX4899001.1"
                     /translation="MMEKTNFEKLRVYRLAESLADGIWSVVLTWNYLAKDTVGKQIVR
                     AADSIGANIAEGSGRGSLEDNRRFIRMARGSLYETQHWLRRSYNRKLLSKQQVDSLKP
                     IIDEL"
BASE COUNT         1273 a         1389 c         1618 g         1262 t
ORIGIN      
        1 ccccaacggc caactcttgc ctctcagttt cgagccaagc aaacgaccgg gagcaaggcc
       61 cccttcctga ctggcaaatt tctgctcgtt ttacgtgtct gccggcgtgc ttggcggccc
      121 ctgtcacgct tagtcaaata cttgaaggaa caaagctaaa acgttcagta acaaaaggtt
      181 tttggtgact gtcgcgttac ggccgtgtta agattcgccc gtcatgcatg gtgatgtaat
      241 catcatacgg cttattttta ccgcaattct gatagtagcg ggatacgtgc ttcgacccat
      301 cgctggggat cgctggattt ccgctggcgt cggcgcgctg atcgccatct gtatcatcct
      361 ttttgagttg cgcattcggc gcgccaccct aaagacgctg attggggcgg ccgtgggctc
      421 gatcatggga atcgtcgggg cgtttctgat cggttctctc ataagtcgtc aagagtcaat
      481 ggcggtggca ccggaagtga agactttcgt cacgctggcg ctcacattct tcatggccta
      541 tgtcggtctt atggtagggg ctgcgaaggg cgactatctc gaactttcgg ctctcggtgg
      601 catcctcagc gaaaaagcct cgcgacgcga tctaaagatt ctcgatacct cggtgattat
      661 tgatggccgg attgcggatg ttgcggagac tggtttcctc actggctccc taatcatccc
      721 gcagttcatc ttgcgtgagt tgcaacaagt tgctgattct cccgattctt caaaacgcca
      781 acgcggtcgc cgcgggctcg acatgctgca tcgcttacag aataacagct cgcttgatat
      841 tcagattgtg gaaaccgatt ttccttcggt gcgtgaagtc gatttgaagt taatcgagct
      901 tggtaagcaa ctggacgccg tcattgttac caacgatttc aatctgaaca aggtctcaca
      961 gttgcggggc gtaagcgtgc ttaatatcaa cgaactggca aatgccttaa agccggtggt
     1021 gctgcccggc gaagcgatgc gcgtttttat cctcaaggaa gggaaagagt acaaccaggg
     1081 tgtggcctac ctcgatgatg gcacgatggt ggtcgtggat aatgcgcgca agttgattgg
     1141 taagaatgca gatatcgcag tgaccagtgt gttgcaaaca accgccggca agatgatctt
     1201 cgggcgtctg tgggaagatg ccgaaaatgg caatggcgag catcgcacta acggctccgc
     1261 gcggcgtcag cctcgcgact ctcgtagcac tcccgggcca gtaaggatta ccgataccga
     1321 acccgtgatc gattagggcg cctcgagcgg cgagagccgg ttgtgcttgg gctgatgagt
     1381 tattcaactt caaattggca atcggcaatc ggcaattggc aatcgagaat cgatccgccc
     1441 ctcatatgaa tgtcgcaatc attgctgccg ccgggcaggg tactcggatg ggcggcaagc
     1501 gagccaagca gtttctcgtg ctcgccggca gaccgatcat cattcacacg atacaagcct
     1561 tcgaagcgtg tgaagcaatt caagaaatca tcgttgtttt accggaagca gacgtaaggg
     1621 acttttccgc agtggctcgg ccttacaatt tgggaaaggt gcgcgctgtt gtgccaggtg
     1681 gagttgcccg cgcgagctct gttctgcatg ggctgcgcgc ggttaacgcg caggagacga
     1741 atatcgtcgc ggtccatgat ggcagccgtc cccttgtgac cgtcgctgag attacccgca
     1801 ccgtcgaagc ggcagaagtc agcggcgcag cgatcctcgt ggcgccgata gttgatacgg
     1861 tgaaggaagt tcgggagggg accgtggttg ctacagttcc ccgggatcat ttgcggcggg
     1921 cgctgacacc gcaatgtttt cgttacgaaa tcctgcgaca agcctacgag caggttgaag
     1981 aggttgacgc cacgcatacg gacgagagtt cactggttga gcgcctgggc gtgaccgtga
     2041 gtgtggttga aggcagttca cgaaatatta aagtgacggg cccagaagac ttggtgatcg
     2101 cagaagcgct gttgaaaacg tagtcagtag cggttgttgg ccaaatgaaa gcttcccctc
     2161 aaggggggtc actttcattt ggccaacaac cactattgac tactgactac ctccaatgtt
     2221 ccgcatcggc atcggcaacg acacacaccg cctggttgaa ggacggccgc tgatcatcgg
     2281 cggtgtgcag ctagccgcta ctcgcggggc ggagggtcat tccgatgctg acgccctggc
     2341 ccatgcgatc gcggacgcta ttttgggagc catttgtgag ggcgatctcg gcgtgcactt
     2401 tcccgaccga gacgaacagt ggaaggatgc tgatagtctg caactgttat cgcgcgtgat
     2461 gtggttggct aacgaacgcg gcttgcgcgt cgtcaacctg gatgcaacgg tacagcttga
     2521 agagcccaaa ctgcgaccct acatgctatc gattcgcgag aacattgccg ccacgctcgg
     2581 cgtcgagctg ggctgcgtga gcgtcaaggc aaaaacaggc gaagggctcg acgctgttgg
     2641 ccagggatta gccatcaccg cgcaggcggc agttttgatg acgagtctgg ccagctgata
     2701 gaggcctcta aggaaaaaaa cttgcgacct caggatgtca ttagaaagaa gcgcgacggg
     2761 gaggaactct cgcgagcgga gattgatttc ttcattgggg gtattaccaa cgacgggatc
     2821 gcggattatc aagtcagcgc gctgctaatg gcgatcttcc tgaacggaat gaatgaggcc
     2881 gaacagcaag ccctgactga agccatgttg cggtccggaa tcacgctcga tttttccggc
     2941 attgccaaac ctaaagcgga caaacattcc acgggcggag ttggtgataa gacttctcta
     3001 attatcgctc cgcttgttgc cgcctgcgga gtttgtgtgc cgatgatttc cggacggggg
     3061 ttgggccaca ccgggggcac gctagacaag ctcgaatcaa ttcccgggta ccgcgttaac
     3121 ctttcgacta aggaattccg tgaagttctg gagcaggtgg ggtttgctat ggctggtcaa
     3181 acagccgagt tggcgccggc cgacaagaaa atgtatgcgc tgcgcgatgc tacctcaact
     3241 gtcgaagcga ttcccctaat agtagcgtcg atcatttcta aaaaaggtgc cgccggtctc
     3301 gatgcgatgg tgatcgacgt taaggtgggc tccggcgcct tcatgagaga ggaagcacgc
     3361 gcccgagcgc tcgcgaaagc tctcgtgaaa acgggaaact cctgtggtat ccgaacgcgc
     3421 gcactactaa ccgacatgaa tcaaccgctc ggtcgcgcgg tgggcaattc actggaggtg
     3481 aaggaatgca ttgagttgtt gcgcggggag gcggaacccg gtgctcgccc tgtcctcgat
     3541 ctttcgttag agctttcagc gcacatgctg gttctcgctc gggttgatga ttcacttgaa
     3601 gctgcgcatg cgcgactaca gcgggttttg gacgcgggta aagccctgga atgttttcgt
     3661 cagaacattg cagctcaggg tggcgacgcg cgggtgtgcg atgatcctgc gagctttctt
     3721 cctttggtga gtgattcgat taaggtagaa tccccgcgct caggcttcat tacagcaatc
     3781 gaaactacgg agatcggtca tgcaattgcc gcattaggcg gtggccgcgt gcgcatcgat
     3841 gacaagatcg atccgacggt tggcttcatc gcggaaaaga agatcggcga caaggttgag
     3901 aagggcgagt tactgggaac cgtctatgct cgggatcaag cgcgagctcg tgagactgct
     3961 gccagaattc aggcgtccta ttgtgtcagc gaggcatctc actcccagca accaacactc
     4021 attaaggaag tgataaacga atgaagcgct ctacctggtc tctttacctg ctggctggtt
     4081 tgattctgct ctccctaatc gcctcctctt gtagcaatca gagttacggt tcggaagata
     4141 cttccaagat ccgcgttggc atcgtttttg acatcggcgg caaggacgac cggtcgttca
     4201 atgcggcagc gtgggccggc gctaaatgtg ctgccacggg aaactggcca gacggaacat
     4261 catgtggcaa acaaaggctg gatattgtgc tgcgcgacat tgaaccgggt aatcctactt
     4321 cgatcgagcc ggcgatgcgc gcttttgccg agcgcaacta cgatctgatc attggcattg
     4381 gcttcgcgca aactccaatc atcgaatcgg tggcgaagga ctaccccaat atccgctttg
     4441 ctgtcgttga cggcgtcagc aatctgccca atgttgcttc actcctcttt aaagaacacg
     4501 agggttcgta tctggtagga atgatggcag ccaggacgac gcagacagga acgataggat
     4561 ttcttggcgg catggacatt gggttgattc atcgcttcaa agaaggttac gaagaggggg
     4621 ccaaagcggt gaatccaaat attcgcgtta ttccaaacta cgtcggcgtt accgatgcgg
     4681 cgtggaacaa cccgggcaaa ggaaaagaga ttgctctggc ccaaatcagc aaaggcgcgg
     4741 acgtgatctt cacagccgcg ggaaactccg gtctcggtgc ctttgacgct gtcgagcagc
     4801 aggggaaaaa gaacggccgg gccacgcact ttgttatcgg cgtagacgcc aatcagaaca
     4861 tggtcaaacc cggttttgtg ctcaccagta tggtgaagcg ggtggacaac gcagtctacg
     4921 acatcatcaa agacgtggtg agcggacagt tcaagaccgg gttccatgtt tacggacttg
     4981 ataaagatgg tgtcggctac gcaatggatg aaaacaacgc gatggacgac aacaagaaac
     5041 cactgatctc accagacgcg atccagcagg ttgaagaagc acgcaaaaag atcattgctg
     5101 gtgagatcaa ggttacggat gctatgaata aatgaaaggc ttttgttctt tgagctttgt
     5161 actttgagct ttgtactttg cgttgtagta atttctttac ctgatccttt cctcacgagg
     5221 gcatgatgga gaaaacaaac tttgaaaagc ttcgcgtata cagattggct gaatctcttg
     5281 ccgacgggat ctggtcggtg gttttgacct ggaattatct agccaaggac acggttggca
     5341 agcaaatcgt tcgtgctgct gacagcatcg gtgcgaatat cgctgaaggc tctggccggg
     5401 gctccttgga ggacaaccga cgattcataa gaatggcacg cggatcgtta tatgagacgc
     5461 agcattggct caggcgttcc tacaatcgaa agttgctgag caaacaacaa gtggacagcc
     5521 tgaaacctat catcgatgag ct
//
LOCUS       DASUEX010000033         2462 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1041434, whole genome shotgun sequence.
ACCESSION   DASUEX010000033 DASUEX010000000
VERSION     DASUEX010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1041434"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="VFV61_00635"
     CDS             <1..426
                     /locus_tag="VFV61_00635"
                     /inference="COORDINATES: protein motif:HMM:NF024979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX4899002.1"
                     /translation="HSAIGNDMKRKPKTYTISAVAEQYSLHPQTLRLYEREGLLKPSR
                     SEGNTRLYTDSDLERLELILSLTRDLGVNLAGVEIIMNMREKMDAMQHEFERFFLYLQ
                     SHAEEFTNFTEPPPEGTSDALVRVTRTAVLRRRTTVSRG"
     gene            627..>2462
                     /locus_tag="VFV61_00640"
     CDS             627..>2462
                     /locus_tag="VFV61_00640"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEX4899003.1"
                     /translation="MKPSPLTTPALAHLTFTVLIFLGIAPFTSAQDLDGAHINGRIVD
                     QNGAVIADALVTATAKRTGALRRVTLNADGRFSIRQLEPGTYRLGVSADGFAAYDKSD
                     LVLIAGQTVFIEVRLALQGLAIDPVTITASDEAAIDTTRTVVGATLTTQEIEALPISS
                     RTALDFLFTLPGITEEPLTTRDLAEDRQSNAAGSPEETGIFAVGGGPAYSNNLTIDGL
                     DNNDDRAARERFQPSVEAIEEIQVITNQFSAEYGRASGGRINIKTRGGGQSLHGRAFA
                     FFKDEALSANTFRNNSLGLKRLPLQEHNPGFTLSGPLLFSHRLRNRTFFFTSYELTKV
                     LDSALIDTLVPVQQNPRFPLPGPTTLAGLRIEDAGSSSASAAIASFISVISTPLTNQT
                     FTTRIDHQFSQTHNGILLYQLGRLKNLRQFSGGNRLAEALQAKSRNSDAVSYSHSYVV
                     SAHTVNQTRLQWSRLTPGVRASGSGSPVVLITLNDSLPANDPGRHTGTLVAGSSTTGA
                     TDRREARFQVQEVLTHLKGAHTLKTGFDFQIIHSTFIDLADASGTFSFASAGDFLANI
                     PSRFRQNFLGQSTQRNRYLGFFAHDEWRAVDNLTVSFGLRYERESI"
BASE COUNT          562 a          729 c          624 g          547 t
ORIGIN      
        1 cattcggcaa tcggaaatga catgaagcgc aagcccaaga catacacgat cagcgcggtg
       61 gcggaacagt acagtctcca cccgcagacc ttacgtctct acgagcgcga aggcctcttg
      121 aagccgtcgc gttcggaagg caacacgcgc ctctacaccg attccgatct cgagcggctc
      181 gagttgatcc tttctttaac tcgtgacctg ggcgtgaatc tcgccggcgt ggaaatcatc
      241 atgaacatgc gcgagaagat ggatgcgatg cagcacgaat ttgagcgctt ctttctttat
      301 ttgcagtcgc atgccgaaga gttcaccaac ttcaccgaac cacctcccga aggcacctct
      361 gacgcactcg ttcgcgtcac gcgcactgcc gtgctccgcc gccgcaccac ggtttctcgt
      421 ggctaattga aatcttgcag tgatctgaaa ctacgtcttc gctcgaaacg attgcgaatc
      481 gccgcgcctc agtgctagcc tccgcacact cttgagtaac actcgactct cttgattaga
      541 gccgtaaaga acgcccgaaa tttaagaata gattttctct aaggtcgcag agattaagcg
      601 cgagccttcc tcacaggaga aatcagatga agccctcacc gctcaccacg ccagcactgg
      661 ctcacctcac tttcacagtt ctgattttcc tcggcattgc gccgtttacc tcagcacaag
      721 atctcgatgg cgcacacatc aacggcagaa tcgtcgatca gaacggggca gtcatcgctg
      781 acgccctggt cacggcaacg gccaagcgaa ccggggctct gcgaagagtc acgctcaatg
      841 ccgatggtcg cttcagcatt cggcaacttg aacccggcac ttacaggctg ggcgtttctg
      901 ctgacggctt tgcggcatac gacaaaagcg atctggtgct gatcgccgga caaacagtct
      961 tcatcgaagt gagactcgct ctgcagggtc tggcgatcga tccggtcacc ataaccgcga
     1021 gcgacgaagc agctatcgat acgacccgga ccgttgtggg cgcaacgctc acgacgcagg
     1081 agattgaagc tctgccaatt tccagtagga cggctctcga tttcctcttc acattacccg
     1141 gcatcaccga agagccgctt acgacgcggg acctcgcgga agatcgccaa agcaatgccg
     1201 cgggcagccc ggaggaaacg ggaatatttg ccgtgggcgg tggtcccgct tactcgaaca
     1261 acttgactat cgacggtctc gataataatg acgatcgcgc cgctcgcgag cgttttcagc
     1321 cttcggtaga agctatcgag gaaatccagg tcattacgaa tcagttttcc gcagagtacg
     1381 gtcgagcgtc gggcggtcgt atcaatatta aaactcgcgg gggtgggcaa agcttgcatg
     1441 gtcgtgcttt cgctttcttc aaggacgaag cgctcagtgc caacactttt agaaacaact
     1501 cactcggtct gaagcgactt ccgttacagg aacacaatcc cggtttcaca ctaagtggac
     1561 cattgttgtt ttcccaccgc ctacgcaacc gaaccttctt ctttacttcg tacgagttaa
     1621 cgaaggtgct tgatagtgcc ctcatcgata ccctcgttcc cgtgcagcag aatccccgct
     1681 tccctttacc cgggccgacg acgttggcgg gtcttcgcat tgaggatgcg ggcagctcgt
     1741 ctgccagcgc cgcaatagcc tcgttcattt ccgttatctc tacgccatta acaaatcaga
     1801 cgttcacgac gcgtatcgat caccagttca gccagacgca taacgggata cttctctatc
     1861 aacttggcag gctgaaaaac ttacgccagt tcagcggtgg taatcgcctt gccgaagccc
     1921 tccaggcgaa gtcgcgaaat tcggatgcag tttcctactc gcacagttat gtagtctcgg
     1981 cacacaccgt gaatcagaca cgattgcagt ggtcaagact cacgcccgga gtcagggcca
     2041 gcggcagcgg cagtcccgta gtcttgatta cgttaaatga ttccttaccg gcgaatgatc
     2101 ccgggcggca cactggcaca cttgtggccg gttcctcgac aaccggcgcg acagatcgac
     2161 gtgaagctcg ttttcaagtt caggaggttc tgacacacct aaaaggagca cacacactaa
     2221 aaaccggttt tgatttccag atcattcact cgacgtttat cgaccttgcc gatgcctcgg
     2281 ggacgttcag ctttgccagc gcgggtgact ttttggcgaa cattcccagc cgtttccgcc
     2341 agaacttcct cggtcagtcg acgcaacgca atcgatacct cggtttcttt gcccacgatg
     2401 agtggcgtgc ggtcgacaat ctcaccgtga gttttgggct gcgttatgaa cgcgaaagca
     2461 tc
//
LOCUS       DASUEX010000034         2510 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_812469, whole genome shotgun sequence.
ACCESSION   DASUEX010000034 DASUEX010000000
VERSION     DASUEX010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_812469"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="VFV61_00645"
     CDS             <1..267
                     /locus_tag="VFV61_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899004.1"
                     /translation="GALDPLTRASLQRQFVELTRRLGKTAIFVTHDLREALRLGSRIG
                     LMHAGRLVLLETPEDFLKSEEEHARAYLETLKLQVEDEPNGGSA"
     gene            303..1826
                     /locus_tag="VFV61_00650"
     CDS             303..1826
                     /locus_tag="VFV61_00650"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX4899005.1"
                     /translation="MLTLIGQHLTLVLISTLIAVVIGIPTGLLLTRKKALRNPVLGIA
                     NVMQTIPSLALFGFLIPLPFLGGIGARTAIVALVVYALLPIIRNTVTGILGVDRNVRE
                     AAVAMGMTDGQILKQVEIPLAMSVILTGVRVAMVISVGVTTIAAAVGAGGLGVYIFRG
                     LRQYDNNLLLAGALPAALMALAADFSLGLLEKRFSLEGKVKKHRGWGFAAGVVTVVVI
                     ALLVTVGIWRAGRGQATGEAGSRVVVASKDFTESILLAEIVAQSLEARAVTVDRQYEL
                     GGNLPHDALVAGRIDLYPEYTGTAYTAILRHPPITDPRAVYDQVKREYAEQFIVDVSP
                     PLGFENTFAILVRGADARRLNIKTISEAVPHARKWRAGFGQDFMTRADGYPGFSKAYG
                     LKFADQPREMDLSLTYIALASNKVDLIAGNSTEGRIAALDFVQLVDDRRYFPPYEAVY
                     LVRKDTLNRVPQLAEVLAKLANSISTEEMRRLNYEVDANKRAPAEVVREWLKPRLAN"
     gene            1844..>2510
                     /locus_tag="VFV61_00655"
     CDS             1844..>2510
                     /locus_tag="VFV61_00655"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX4899006.1"
                     /translation="MKKLLPVIITCFAVLANTNAQNATSRVTLIKAGRLVDVRAGRVL
                     ENQGILIQGERIKLVGPLAEVQRSAPSTVPVIDLSRATVLPGLADCHTHILLQGDITA
                     ADYDEQLLKESIPYRTIRATVAARIALLNGFTVMRDLETEGAMYADVDVKQAINRGVV
                     PGPRMFVATRAFSATGMYPLQGYSWELRMPEGVQIVDGPDNIRRAVREQVKFGADWIK
                     FYAD"
BASE COUNT          536 a          661 c          738 g          575 t
ORIGIN      
        1 ggcgcgctcg atcccttgac ccgtgccagc ttgcaacgcc aatttgtaga gctcacgcgt
       61 cgactcggca agactgcaat cttcgtaact cacgatctgc gtgaagctct acggcttggt
      121 tcgcgcatcg gtctcatgca cgccggacga ttggtgctgc tggaaacgcc tgaagacttt
      181 ctcaaatctg aagaggaaca cgcacgcgct tatttggaaa cgctcaaatt gcaggtggaa
      241 gacgagccaa atggaggaag cgcgtgaatt tttggcaatt ccttcaacaa aactggagcg
      301 aagtgctgac actcattggc cagcacctca cactcgtttt gatctcaact ctgattgctg
      361 ttgtgattgg tattcccacc ggcctccttt tgaccaggaa aaaagcgctg cgcaatcccg
      421 tcttgggaat tgccaacgtc atgcaaacaa ttcccagcct ggcactgttt gggtttctca
      481 ttcctttgcc tttccttgga ggcatcggcg cgcgcacggc aatcgtggcc ctcgttgttt
      541 acgccttgct gccgatcatt cgcaatacgg tcaccggaat tctgggcgtc gatcgcaatg
      601 tgcgtgaagc ggcagtggcg atgggtatga cggacggtca gattctgaag caagtagaaa
      661 taccactcgc gatgagcgtg attctcaccg gagtgagagt agcgatggtg atctcagtgg
      721 gagtaacaac tatcgccgcc gcagttggcg ccggcggcct tggtgtttac atcttccgcg
      781 ggctgcggca gtacgacaac aatcttctac tggcgggcgc attacccgcg gcgttaatgg
      841 cgctcgccgc agacttttcg ctcggtctgc ttgagaagcg gttttccctg gaaggaaaag
      901 ttaagaagca ccgtggttgg ggctttgcag cgggcgttgt tacggtagtg gtgattgcct
      961 tgctggtgac ggtggggatc tggcgcgcgg ggcgcggtca ggctacaggg gaagccgggt
     1021 cgcgcgtcgt cgtcgcctcg aaggatttta ccgaatcgat tctgctggca gagattgtgg
     1081 cgcaatcttt ggaagctcgc gccgtgacgg tcgatcgcca gtatgagttg ggtggcaatc
     1141 ttccgcacga cgccctcgtg gccggaagaa ttgatttata cccggaatac accgggaccg
     1201 cttacactgc aatcctgcgt catccgccaa ttaccgatcc gcgcgccgtc tatgatcagg
     1261 tgaagcggga gtatgcggaa cagttcattg ttgatgtcag tccgccactc ggatttgaaa
     1321 acactttcgc gattctcgtg cggggcgctg atgcccggcg gctaaacatt aaaaccattt
     1381 ctgaagcagt gccacacgca cggaaatggc gcgcggggtt tggtcaggat tttatgacgc
     1441 gcgccgatgg ctatcccggt ttttccaagg cgtatggctt aaagttcgct gaccaaccac
     1501 gggagatgga tctgtcgcta acctatattg ccctggcatc aaacaaagtt gacttgattg
     1561 caggcaactc taccgaaggc cgcattgccg cgctcgactt tgtgcaactc gtcgatgacc
     1621 ggcgttactt tccgccttac gaagccgttt acctcgttcg caaagacaca ctgaatcgcg
     1681 tgcctcagct tgcggaagtg ctggcgaagc tggcgaactc gatttcaacg gaagagatgc
     1741 gacggctcaa ttatgaagtc gatgccaata agcgcgctcc ggctgaagtg gtgcgggaat
     1801 ggctgaagcc gaggcttgcc aattgaagct gaaaggaaga gtcatgaaga aactactccc
     1861 cgtaataatc acgtgtttcg ccgttctggc taacaccaat gcccaaaatg caacctctag
     1921 agtcacgctg atcaaggcgg gccgcctggt tgatgtgcgg gctgggcgcg tactggagaa
     1981 tcagggaatt ctgattcagg gcgagcgcat caagctggta ggtccgctag ctgaggtaca
     2041 gcgaagtgct ccaagcacgg tgccggtaat cgatctttcg cgagcgacgg tgttaccggg
     2101 cctggcggat tgtcacacgc atattttgct gcaaggcgac atcaccgccg ccgattacga
     2161 tgagcaactc ctcaaagagt cgattcctta ccgaacgatt cgggccactg tcgctgcgcg
     2221 tatcgccctc ctcaatggtt tcacggtaat gcgtgacctg gaaacagaag gcgcgatgta
     2281 cgcggacgtt gacgtcaagc aggcaatcaa tcgcggcgta gtccctggtc cgcgcatgtt
     2341 cgtggcgacg cgcgcatttt cggcgacggg tatgtatccg ctgcaaggct attcctggga
     2401 actgcggatg ccggaagggg tgcagattgt tgatgggccg gacaacattc gtcgtgcagt
     2461 acgcgaacaa gtaaagtttg gcgccgattg gatcaagttc tacgccgatc
//
LOCUS       DASUEX010000035         2217 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1150750, whole genome shotgun sequence.
ACCESSION   DASUEX010000035 DASUEX010000000
VERSION     DASUEX010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1150750"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..1598)
                     /locus_tag="VFV61_00660"
     CDS             complement(57..1598)
                     /locus_tag="VFV61_00660"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HEX4899007.1"
                     /translation="MTKPLYHGAINIHGAAPSPDNKLLACTGRGTSNVYLIDLETMKV
                     IGNTPNSQASEQTNGERITSGVMVGREPHEPTFSRNGKEIWVTVRGENRIAILDTASA
                     IKESRGVASRAVRLYVNCLNGPAQVWFSADGRLAFVASQKTSQVEVFETNFDRDGFSR
                     PKRRVLLDIKAQDPFAFTPFQKTTPDGKELWLSHKLADAVSVWSVGQEPKLVDAVPLG
                     KLARPNHVEFVENAKGKVVYASLARVDDGGPGKTASSQIAIIDRSAAPGSRKVVGNFF
                     SQAREAHGLWTNPENTLLYIAHEQDELPNTPNAGQTVCTAFDVSDPLKPSFIAQIPLG
                     ELALPSGKLRNKKSINLVYVRPGARSQTAYRRNSMRTTIVTLMIVGAITAQLAYAQHQ
                     HGTVESQNAPLSAGAEPFARQMEAGMEKMMNDMHAPGYTGNPDVDFLAMMIPHHEGAV
                     EMARLVLIHGKDPLTRRLAEEIIASQTAEIAAMKQRVAILRRSDDPNPDGFPAIHGTR
                     GAKPQ"
BASE COUNT          440 a          596 c          652 g          529 t
ORIGIN      
        1 ggaatctgaa ctgctaaaag agttttcaca cggtggctac atgccgcatc gctctcttat
       61 tgtggcttgg cccctcgcgt gccgtgaatg gcagggaagc cgtctggatt cggatcgtcg
      121 ctgcggcgca gaatagccac gcgctgcttc atcgcggcaa tctccgcggt ctgtgaggcg
      181 atgatctcct cggcgagccg gcgggtcagc gggtcctttc cgtgaatcag caccagtcgc
      241 gccatttcta ccgcgccttc gtggtgcgga atcatcatcg ccaggaaatc aacatcggga
      301 ttgccggtgt aaccgggagc gtgcatgtcg ttcatcattt tttccatgcc cgcctccatt
      361 tggcgcgcga agggctcggc gcccgcggaa agcggtgcat tttgagattc cacagtgccg
      421 tgttgatgct gcgcataggc taactgcgcg gttatcgcgc ctacgatcat gagcgtgacg
      481 atcgttgtcc tcatactgtt ccttctatag gcggtttggc tacgtgcgcc ggggcgcaca
      541 tagacgaggt tgatgctctt cttattgcgc agcttgccgg aagggagcgc gagttcgccg
      601 agggggattt gcgcgatgaa gcttggcttg aggggatcgc tgacatcgaa ggcagtgcaa
      661 acagtctgcc cagcgttggg cgtattcggc agttcatctt gttcgtgggc gatgtagagc
      721 agcgtgtttt ccggatttgt ccacaagccg tgcgcttcac gcgcttggct gaaaaagtta
      781 ccgacgactt ttcggctccc tggcgccgcc gagcgatcga taatcgcgat ctgactcgaa
      841 gccgttttac cggggccacc gtcgtccacg cgggcgagcg acgcatagac aaccttgcct
      901 ttcgcgttct ccacgaattc aacgtgattg ggccgggcga gcttgccgag gggaaccgca
      961 tcgaccagct ttggctcttg accgacggac cagacagata cggcgtccgc aagcttgtgc
     1021 gacagccata gctctttgcc atccggtgtg gtcttttgaa acggagtgaa ggcgaaaggg
     1081 tcttgcgcct tgatatcgag aagcacccgg cgcttcggac ggctgaatcc gtcacggtca
     1141 aagttggtct caaaaacttc gacctgcgaa gttttctgcg acgccacgaa ggcgagtctg
     1201 ccgtcggcag agaaccagac ctgagccgga ccgtttaagc aattcacata cagtcgcacg
     1261 gcacgcgaag ccacgccgcg ggattctttg atcgcgctcg cggtgtcgag aatcgcgata
     1321 cgattttcgc cgcggaccgt cacccagatt tccttgccat tgcggctaaa agttggttcg
     1381 tgtggctcgc gaccgaccat gaccccgctg gttatgcgct cgccgttggt ctgctcgctc
     1441 gcttgtgagt tcggcgtatt gccgatgacc ttcatggttt cgagatctat aagatagacg
     1501 ttactggtgc cacgcccggt gcaggccaaa agcttgttgt ccggcgatgg ggcggcgccg
     1561 tgaatgttga ttgcgccgtg atacagcggc ttggtcacca tcgcgagatg agtgggagtt
     1621 actcctcccg taaccagccg gaaaggcggt cgcgggtctt cgtcaaatga tgtgagattg
     1681 attgttgtgg caactgtgtt cgtgtttgga tcaattacgc tcagcgtgtt ggagtcttcg
     1741 ttgcagatga tgactcggtc tcggttgctt gcgccggcaa cgccctgagc gaatgcacgg
     1801 tcaaatggaa atgccgcaag cgtcgccgct gcgctaacgt taataatgaa ttcgcgtcgg
     1861 gtccagtccc tcatattacc ctctcaagtt attgaacatc gctgctgttc aggtaaatct
     1921 aaaaacgacc ttcgtatgtc aatttaaagc acttttagcc tcaagggcca aacaaatcag
     1981 gacgatgaag tgtcgcgcaa cccgggttcc tggattccgt ttcactctat ccaggctaaa
     2041 aaaaaccaac agcgaggatc tgagaaagat ttctcccgga gtttatcctg agcgttgccg
     2101 aaggagtcga aatgacaagc gcgcttttgt ggatttgcgc gagtcgcagc aatttactgg
     2161 cttacgtgca gcataaaagg ggccgggtcg gttgccctta cacgcgatca gaatttt
//
LOCUS       DASUEX010000036         2095 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1266952, whole genome shotgun sequence.
ACCESSION   DASUEX010000036 DASUEX010000000
VERSION     DASUEX010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1266952"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1069)
                     /locus_tag="VFV61_00665"
     CDS             complement(<1..1069)
                     /locus_tag="VFV61_00665"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HEX4899008.1"
                     /translation="MENSLAITLIGGGAVSTQDAASILCVVGIIAAIYLIYRTSLERI
                     QAKTREAEAVGRLHLATAEALATAIDAQDQTSHYHVRRVQIFAVGMGEVLKLAKPEIE
                     ALRAGALLHDVGKLAVPTHILNKPGQLTAAEFEKMKIHTIVGAELLSRVDFPYPVIPI
                     VRHHHEHWNGAGYPDGLRGEQIPLTARIISVVDCFDAVREDRPFRRGMSRAEAIAMLL
                     RGAGNHFDPRVVELFIEHLPGFEAQIAKLGLALPEPDSPAAEPVALAAVDVQQTRERG
                     CYMAYDQIRNAHREVYALYEIARTFGSSLDINETLEILVDKVGHVVSFDTCVVYVYDE
                     MRGYATAAHVVGKNASLLTTKC"
     gene            1653..>2095
                     /locus_tag="VFV61_00670"
     CDS             1653..>2095
                     /locus_tag="VFV61_00670"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4899009.1"
                     /translation="MAKTTSTGLQTQTPSGYIELLRGNRAFRQVWLGQVVSQLGDWFN
                     TIALYTIILNLTGSGRDIGLLLVARFVPSFIFGPSSGVLADRFSRRSIMIYSDLLRAI
                     VVLGFLFVRRADQLWLIYVLTVLQLGLSTFFEPAKTAAIPSLVADR"
BASE COUNT          456 a          581 c          580 g          478 t
ORIGIN      
        1 cacacttggt agttagtagt gaggcattct tgcccacaac gtgcgcggca gtcgcgtaac
       61 ctcgcatctc gtcatagaca tacacgacgc aagtatcaaa cgaaacaacg tgtccaacct
      121 tgtccacgag tatttcgaga gtttcgttaa tgtcgagcga agagccaaaa gtacgggcaa
      181 tctcgtagag cgcgtaaacc tcgcggtggg cattcctaat ctgatcgtaa gccatgtagc
      241 agccgcgctc gcgcgtttgt tggacatcga cagcagccaa agcaactggc tcggccgccg
      301 gactatcagg ctcgggcaac gccagaccca atttggcaat ttgcgcttcg aaacctggca
      361 ggtgttcgat aaagagttct accacgcgcg gatcgaagtg gttaccggcg cctcgcagca
      421 acatggcaat agcttcagcg cgcgacatgc cccgacggaa cggccgatcc tctctaaccg
      481 cgtcaaagca atcaacaacc gagattattc gcgctgttag tggaatctgt tcaccgcgca
      541 agccatcggg ataaccggca ccattccaat gttcgtggtg gtggcgcacg atgggaatta
      601 ccggataagg aaagtccacg cggctgagca actctgcgcc cacgatcgtg tgaatcttca
      661 tcttctcaaa ctcggccgcg gtcagctggc cgggcttgtt gagaatatga gtgggcaccg
      721 ccagcttccc gacgtcgtgc agaagtgccc cggcacgtaa agcctcaatt tccggcttgg
      781 ccaatttgag aacttcaccc attccgacgg cgaagatctg cacgcgacgg acgtgatagt
      841 ggctggtttg atcctgtgca tcaatggccg ttgccagagc ttccgcagtc gcaaggtgca
      901 atctgccgac cgcctccgct tcccgcgtct tagcctgtat ccgctcgagt gaggtgcgat
      961 agatcaggta gatggcggcg atgatgccga ccacacagag tattgacgcg gcgtcctgag
     1021 ttgaaactgc gccaccacca atcagtgtga ttgccaggct gttttccatt tgcaagggtg
     1081 tcgcttcggc caataaatgc agttagccga aagcgaaact gggagcaact cagcttcgct
     1141 tccggcgtag cgggcaagcg acgaacgata gtgatcgatt cgccatgctg ggtgagattt
     1201 cggaccgccg tctaaacatc ttcgcggttt caaatttggt gcggctcgga ctccgtgcgg
     1261 tgagtccgtg aagcccgaag gcctctcagc cgcgactaac tctttctcaa ttgatgtgcc
     1321 gtaggctgct tgcggtcatt tcagccgcaa aagccagtgt tttcgcttgg agaatcagtc
     1381 agagggtttg aattacttga aagcacaatg ggacgcttca aagtgacagg tcacgttgcc
     1441 acattcagtc agaacgattg ccgaatgccg attggcgagt ggcgattggt tggagtcagt
     1501 accaccaggc ggatagccgg tgggccctgg gaatgccgat tagcttcttt cgttacgaac
     1561 taatcgccaa ttggcaatcg acaatgagtt gacagtcgca aatcagaaag gcaccctgct
     1621 aatcaccagg aaaaccagtt aggtggcaag cattggcgaa gaccacgagc accgggctcc
     1681 agacccaaac tccatccgga tacattgaac tcctgcgcgg gaaccgtgcc tttcgtcagg
     1741 tatggctcgg tcaggtcgtc agccagttgg gcgactggtt caatacgatc gcgctctaca
     1801 cgatcattct gaatctcacc ggctcagggc gagacatcgg gctgctgctg gtggcacgct
     1861 ttgtcccgag tttcattttt ggaccgtcgt caggggtgct cgctgatcgc ttcagccggc
     1921 gctcgatcat gatctattcg gatctgttgc gagccatcgt cgtcctcggc ttcctgtttg
     1981 tccgtcgtgc cgaccagctc tggctaattt atgttctgac cgtcttgcaa ctaggtcttt
     2041 ccacgttttt tgagcccgcg aagacggcgg ctattccttc gctggttgcc gatcg
//
LOCUS       DASUEX010000037         2432 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1041792, whole genome shotgun sequence.
ACCESSION   DASUEX010000037 DASUEX010000000
VERSION     DASUEX010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1041792"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..652)
                     /locus_tag="VFV61_00675"
     CDS             complement(<1..652)
                     /locus_tag="VFV61_00675"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX4899010.1"
                     /translation="MRAMILSAGYGTRLWPLTEDRTKPAIPILGKPLVGYVAEYLARY
                     GWRDILVNLHHRPDSVRKALGDGSQFGVKLQYIDEPVILGTSGALDNARSYFDSETFL
                     VINGKLICDLDLDAALARHRESQAIATLVLLPNTKYERFTVVETANGFLKGFAGMPGP
                     PSASLEPPLMFTGIQILEPRIFEYIPRGVFSHTTTDVYPQAILQGEQVAVHVASGTW"
     gene            complement(705..1295)
                     /locus_tag="VFV61_00680"
     CDS             complement(705..1295)
                     /locus_tag="VFV61_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899011.1"
                     /translation="MERPNENLLSRLEQSLRQSRAARMHLAARLRELEVEADAVRAEL
                     AEMDTLASQSEAAIYRLLSSVLSSSSLAAGLPSDLELENAMRQSSGHSRPAMGMEGRE
                     ARRQEIPAIRSNVEAVSDRFLDRTIPQAVTLLLREEGGPLHVNEIYNRLLEGGFKFTG
                     ANPTISVAVSLNRNSRFRKVSPGTFDLMIRDASKAS"
     gene            complement(1432..>2432)
                     /locus_tag="VFV61_00685"
     CDS             complement(1432..>2432)
                     /locus_tag="VFV61_00685"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX4899012.1"
                     /translation="IAMEDARISARRIFSLIAAELAQVEAEFERQARSNVQVIDFIAD
                     YLRASGGKRVRPALTILANYAVGGNGGRYNSIRMATVMEFLHTATLVHDDIIDSAETR
                     RDRPTVNSVYGNETAVLMGDWLYMSAFETSLAERSLPILDILTAVTRKMTEGELLQLD
                     LLGRTDVSEPAYLDVLQRKTAYLFSACCEVGAILGEANDTQRTALRDFGLNLGTAFQL
                     TDDLLDFTGTDDVLGKAAGVDLLAGKVTLPLIYLLESDPSMTARLQTVITDANYEQVT
                     RAELLEAVDRVGGLERARARADEFAEAARSALSVLPDSEYCDALRSIPTYVLERER"
BASE COUNT          541 a          692 c          699 g          500 t
ORIGIN      
        1 accaggtgcc tgacgccacg tgcactgcaa cctgctcgcc ctgcagaatc gcctgcgggt
       61 aaacgtcggt tgtagtgtga gagaaaactc cgcgtgggat gtattcaaag atgcgcggct
      121 ccagaatctg aatgccggtg aacatcagtg gcggttccag cgaggccgag ggcggccctg
      181 gcatgccagc gaatcccttg agaaatccat tggcagtctc aactacggtg aagcgctcat
      241 acttggtatt gggcagcagg accagcgttg cgattgcctg cgactcgcga tgcctcgcga
      301 gcgccgcatc gagatcaaga tcgcagatga gtttgccatt gatgacgagg aaggtttcag
      361 agtcgaagta agagcgagcg ttatcaagcg cgccactcgt tcccaggatt accggttcgt
      421 cgatgtattg aagtttgact ccaaactgac tgccatcgcc cagcgccttg cgaacagagt
      481 cgggacggtg atgcaggttt acgaggatgt cccgccagcc gtaacgagca agatactcag
      541 cgacgtagcc gaccagcggt ttgcccagga taggaatcgc aggtttggtg cggtcctccg
      601 ttaacggcca cagacgggtg ccatatccgg cagaaagaat cattgcgcgc atgcaaggta
      661 ttctaagcgg agggaatgaa attgagatag gaagagtgaa cgacttaaga cgctttggaa
      721 gcgtcgcgaa tcatgaggtc gaaagtgccg ggcgagactt tacgaaagcg gctgttgcga
      781 tttagagaaa cagcgacact aatcgtcgga ttggctccag tgaacttaaa tccgccctcg
      841 agcagacggt tgtagatttc gttcacgtgc agggggccac cttcttcgcg taagagcaac
      901 gtaacggcct ggggaatagt acgatcgaga aaacgatcgc tgacagcttc aacattactt
      961 cgaatcgcgg gaatctcctg gcgacgagct tcacgaccct ccattcccat ggcggggcga
     1021 gaatgaccag aagactggcg cattgcattt tccagctcca gatcggaggg caggccagcc
     1081 gcgagactac tcgacgaaag cacagaggaa agtagccggt agatggctgc ttccgactgg
     1141 ctcgccaacg tatccatctc agcgagttcg gcccgcaccg cgtcggcttc cacttcgagc
     1201 tcgcgaagcc gggccgccag atgcatccgc gccgcccgcg attgccgcag gctttgttcc
     1261 agccgggata gaaggttttc gtttgggcgt tccatcgggg ggaatcttag agggatttgg
     1321 cccgttagac aatacttttt gccgtgtggg taaccctcaa tcgtccctga cggggactct
     1381 ctcgctttcg gcgtgaagcg gcggcaagtc agccttccgg actgagcact tctaccgctc
     1441 gcgctccaac acatacgtag gaatagatcg aagcgcatcg cagtattccg aatccggtaa
     1501 cacactcagt gcacttcgtg cggcctcggc aaactcatca gcacgcgccc gggcacgctc
     1561 aagacctccc acgcggtcca ccgcctccag caattcagcc cgcgtcacct gttcgtaatt
     1621 tgcgtccgtg attacggtct ggagccttgc cgtcatcgag ggatcgcttt ccagcaggta
     1681 aatcagaggc agcgtcacct tgcccgcgag aagatccaca ccggcagcct tgcccagtac
     1741 gtcatcagtt ccggtgaagt ccagcaggtc gtcagtcagc tgaaatgcgg tgcccagatt
     1801 caagccaaag tcgcgcaagg ccgtgcgctg tgtgtcgttg gcttcgccca ggatagcgcc
     1861 cacctcgcaa caggcgctga agaggtacgc agtcttgcgc tgtaagacgt cgaggtaagc
     1921 tggctcagag acgtctgtgc ggccgagcag atccaattgc agcagctctc cttcggtcat
     1981 cttgcgcgtg acggcagtaa ggatgtcgag tatcggcagc gagcgttccg ccagactcgt
     2041 ttcgaaagcc gacatataaa gccagtcgcc catcaacact gctgtttcgt ttccataaac
     2101 cgaattcacc gtcggtcgat cgcgacgcgt ttcagcgctg tctatgatgt cgtcgtggac
     2161 gagcgtcgcc gtatgcagaa actccatgac agtggccatt cggattgagt tgtagcgtcc
     2221 gccgttgccc cctacggcgt agttcgcaag tatcgttaaa gccgggcgaa ctctcttccc
     2281 tccagaggcg cgcagataat cagcgatgaa atcaatcacc tgaacgtttg aacgcgcttg
     2341 tcgctcaaac tcggcttcca cctgagcgag ctccgcagcg atgagactaa aaatccggcg
     2401 cgcggaaatt cgcgcgtcct ccatcgctat tg
//
LOCUS       DASUEX010000038         2249 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_126062, whole genome shotgun sequence.
ACCESSION   DASUEX010000038 DASUEX010000000
VERSION     DASUEX010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126062"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..792)
                     /locus_tag="VFV61_00690"
     CDS             complement(<1..792)
                     /locus_tag="VFV61_00690"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEX4899013.1"
                     /translation="MSQSSEMSGKAEFDPSVTTCAKCGTPMPVGLRFCRNCGFRLGEG
                     PAEYTETVRFPNGQLGATQQPFSPNYAAPLAHVASGAFGKPRKRMSGMSWMFIGLLVF
                     FLAAAAFTAIVTPIRRNVTTISVPEKPRAFIGVNDFETGEGGVTFNNVEPPGSPADKA
                     GLVGGDIITSFDGHVVTTEDEMTELMGNTTIGKPVDVIYLRDGEQKTTKLTPVTRADF
                     ERLEREFANRAGGKGRFGFDDGDTERVPIPGTKMFGIRLDDISPNL"
     gene            1134..1349
                     /locus_tag="VFV61_00695"
     CDS             1134..1349
                     /locus_tag="VFV61_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899014.1"
                     /translation="MEVTKALAALQPLYGKDNVEVITMDGRRVRGIVRSMKTTQALTT
                     PSVKIECSNGEVVKIPFPAIAEIKDYN"
     gene            1454..2062
                     /locus_tag="VFV61_00700"
     CDS             1454..2062
                     /locus_tag="VFV61_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899015.1"
                     /translation="MALMIENLGGRVATVWSELRPTTRGLVERALQLSNANSPQSRNP
                     PYDPRADLELSRLLTALDDRAAETTAAADSEESEQLRHVANTCATVLQEKTQSAEVFA
                     QLVRRADQQRDYKRIDVLADALNSRFAPSEICELARSEDVIVRALANEALSQFPTSVL
                     VGLLSDPVDSEIARDALRRQAMEFGSDEARQIINALDQVEEL"
BASE COUNT          526 a          621 c          617 g          485 t
ORIGIN      
        1 caaattcggc gagatatcat cgagccggat gccaaacatt ttggttccgg gaatcggcac
       61 gcgctcggtg tcaccgtcgt caaatccaaa acgccctttg ccgccggcac ggttggcaaa
      121 ttctctttcc agccgctcaa aatcggcgcg cgtgactggc gttagcttcg tcgtcttttg
      181 ctcgccgtcg cgcaggtaaa tcacatctac cggcttcccg atcgtagtgt ttcccattag
      241 ttcggtcatt tcgtcctcag tggtaacaac atgaccgtcg aagctggtga tgatgtcgcc
      301 accaacgaga ccggctttgt ccgcgggcga tcccggcggc tctacattgt tgaacgttac
      361 gcctccctca ccagtctcga aatcattaac accaataaaa gcacgcggct tctcggggac
      421 agaaatcgtt gtaacgttgc gacgtatcgg cgtgacgatg gcagtaaacg ccgcggcagc
      481 gagaaagaaa acaagcagac cgataaacat ccaactcatc ccgctcatcc gcttgcgtgg
      541 tttgccgaaa gcgccagagg ctacatgcgc taagggcgct gcatagttgg gcgagaatgg
      601 ttgttgcgta gcgccgagtt gaccgtttgg gaagcgcacg gtttctgtgt actcagccgg
      661 cccttcgccc agccggaagc cacaattacg gcagaagcgc agacccacgg gcataggcgt
      721 gccgcatttt gcacaagtag taacacttgg atcgaattct gcttttccac tcatttctga
      781 actctgcgac atgagacttt caccagtcca agggttggcg ctgaaatcgc tccggatggg
      841 tatacgccaa aaactgccta ccagttgcat cagcgagcag ccacaacgca ccgcttatag
      901 taagtcaaaa acgagcgtga tttgtagtgc gggcgctaag tttctcaatc gtggtagggt
      961 acctcgaaaa atgcgttttc ccgcgtttcg gttgcatttc gcttaatcgg agggccgaat
     1021 ctcccttttt ccgagatggc gcgaaacggg cccattagac atctgcgtat gatacgtgca
     1081 gaatcgcatc tgttttcccc tggcttggaa atgaacccga aggaacaatt actgtggaag
     1141 tcacaaaagc tctcgccgct ctccaaccgc tgtatggcaa ggacaatgtc gaagtgatta
     1201 cgatggatgg ccgtcgagtc cgcggtattg tgcgcagcat gaagaccacc caagccctca
     1261 ccaccccgag tgtgaaaatc gaatgctcaa acggagaggt cgttaaaatc ccttttccgg
     1321 cgatagctga aatcaaagat tacaactaag gcaattacgc gctgttttgg gagcacggca
     1381 ttctcgcgtt tcctgagacc atcgcgtata gtacgccgtt cataaatggg gataacgagg
     1441 agagctcagg aacttggcat taatgattga aaacctcggc ggacgggtgg ccacagtttg
     1501 gtcggaactg cgaccaacga cccgcggtct cgtcgaaagg gcgttgcagc tctcgaatgc
     1561 aaattcaccg cagtcgcgaa atccgcccta cgatccgcgc gcggatctgg agctcagccg
     1621 cttgctgaca gcactcgatg atcgcgcggc agaaaccact gctgcagccg acagcgaaga
     1681 gagtgagcag ttgcgtcacg tcgccaacac ctgcgccacg gttttgcagg agaagacgca
     1741 gtcggccgag gtctttgcgc aactcgtgcg ccgggcggat cagcaacgcg actacaaacg
     1801 catcgatgtg ctggcggacg cgctcaattc gcgctttgca ccgagtgaga tttgcgagct
     1861 agcgcgttcg gaggacgtga ttgtcagggc cttggccaac gaagctctct cgcaattccc
     1921 gacttcggtg ctggtaggat tgttgagtga tcccgtggat tctgaaattg cgcgcgacgc
     1981 cctacgtcgc caggcgatgg agtttggttc agatgaggcg cggcaaatca tcaacgcgct
     2041 ggatcaagtg gaagagctct agcctgccta aagagttacc gccaagacgc cggctgatca
     2101 gcgccgagac gcaaagccac cgagacgcaa agttagtcag gccataaagg gactgcctta
     2161 agtatgtttg ggtttcgcgt tgggatagct atgtcccgca agctcatgaa agaacaactc
     2221 gcttcacccc ctccttcagt agcgcgaca
//
LOCUS       DASUEX010000039         2395 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_11772, whole genome shotgun sequence.
ACCESSION   DASUEX010000039 DASUEX010000000
VERSION     DASUEX010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11772"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..831
                     /locus_tag="VFV61_00705"
     CDS             <1..831
                     /locus_tag="VFV61_00705"
                     /inference="COORDINATES: protein motif:HMM:NF014895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HEX4899016.1"
                     /translation="AAVIGAEDPREFFVRRFSEGVGRIAAAFYPKPVIVRTSDFKTNE
                     YARLLGGSEFEPAEENPMLGFRGASRYYDPRYAAGFALECAALLRVRKEMGLTNVKIM
                     IPFCRTVAEGKRVVDVMAEHGLKQGDEGLEIYAMCELPANVTRADEFLRVFDGYSIGS
                     NDLTQLVLGIDRDSGTVSHLFDEKDRAVLGLIAQVIDEAHRAGKPIGICGQAPSDFPD
                     FARWLVEQGITSISLNPDVAIKTQLVIADEEKRSLGPPPMKTKVPLVVTNSRARASLM
                     "
     gene            847..1194
                     /locus_tag="VFV61_00710"
     CDS             847..1194
                     /locus_tag="VFV61_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899017.1"
                     /translation="MPSGLLIGLVLIPSMLLAYVVLTTFYGANRAYIAISWLPLAFIL
                     TIWIFIQLLQFQHLSDQWWRDLAGTIHWASLFQTGLGMSLIARTLWRHESAIGVLLAT
                     LVSLAPFFVRFGT"
     gene            1312..2325
                     /locus_tag="VFV61_00715"
     CDS             1312..2325
                     /locus_tag="VFV61_00715"
                     /inference="COORDINATES: protein motif:HMM:TIGR02822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEX4899018.1"
                     /translation="MKAWLLHKPGPVAERPLTIADVITPAPGDDELLVRVQACGICRT
                     DLHVIEGELPERRSPLIPGHQIVGRVTARGARVRDFAVGDRVGLAWLNHTCGACEFCC
                     SKRENLCEQPQFTGWTVDGGYAEDALAPASFTYHIPDDFEAAQAAPLLCAGIIGYRCL
                     RLTGINDNEWDGGRLGLYGFGAAGHISIQLARARGAEVFVCTRDRERHQALAHELGAT
                     WVGDASDEPPVKLDAAIIFAPAGELVPVALKAVRKGGTVVLGGIHMSVIPAFDYSLIY
                     GERVVRSVANNTRADGREFLAEAARIPVRTHVETFTFDQANEALLALKNDAIRGAGVL
                     LIG"
BASE COUNT          501 a          636 c          690 g          568 t
ORIGIN      
        1 gcagcagtta tcggcgctga agatccgcgt gagttttttg ttcgtcgctt cagcgaaggt
       61 gtcggacgta tagctgccgc tttctaccca aaaccggtga tcgttcgcac cagcgatttc
      121 aagaccaatg agtatgcgcg gctgctgggc ggaagcgagt ttgaacccgc tgaggaaaat
      181 ccgatgctgg gttttcgggg tgcttcccgc tattacgatc cgcgctacgc tgccggcttt
      241 gcgcttgaat gtgcagcgtt gttacgcgtg cgcaaggaaa tgggtctgac caacgtcaaa
      301 atcatgattc ccttttgccg taccgtcgca gagggaaagc gtgtagtcga cgtcatggcc
      361 gagcacggct tgaagcaagg cgatgagggt ttggagatct atgcaatgtg cgagctcccg
      421 gccaacgtca ccagagccga tgagtttttg agagtgttcg atggctactc gattggctca
      481 aacgatctga cgcagctcgt attgggaatt gaccgcgact caggcaccgt ttcgcatttg
      541 ttcgacgaga aggaccgcgc tgttctggga ctgattgcgc aggtaatcga tgaagcccat
      601 cgcgccggaa aaccgattgg catctgtggc caggcgcctt cagacttccc ggactttgcc
      661 cgctggctgg tggaacaggg tattacttcg atctcgctca atcccgatgt ggcaatcaag
      721 actcaattgg ttatagccga cgaagaaaag cgatcgctgg gccctccacc aatgaaaaca
      781 aaagtgccat tggttgtaac aaactcacgg gcgcgtgcca gtctcatgtg atgaagaggt
      841 aacgctgtgc cctcgggatt gttgatcggt ctagtcctga taccttccat gctccttgct
      901 tatgtggtgc tgacaacatt ctatggagcg aatcgggcct acattgcgat ctcgtggctg
      961 ccgttggcgt ttattctgac aatctggata ttcatccagc tgctgcagtt tcaacatttg
     1021 tctgatcaat ggtggcggga tttggctggg acgatacact gggccagtct attccaaacc
     1081 gggttgggaa tgagtttgat tgcccgcacg ctctggcggc acgaaagcgc tatcggtgtg
     1141 ttgctagcga cgctggtctc cctggcccca ttcttcgtgc ggttcggcac ctaatctcaa
     1201 gggcaatgag tcgcgcttgt ttgcgctgat tactctcgtg atattagaaa atactaaacc
     1261 acgaaattgc acggacaaac actgaccaga ctcgatctaa agtagaacaa aatgaaagcc
     1321 tggctgctcc ataaaccggg gccggttgcc gaacgtcctc tcacgattgc cgacgtaatc
     1381 actcctgccc ctggtgatga cgaactgctg gtgcgcgttc aggcgtgcgg catttgtaga
     1441 acggaccttc acgtcattga aggcgaactt cccgaacgtc gctcgccact gattcccggt
     1501 caccagattg tgggccgcgt cacggcgcga ggtgcgcgag tcagagattt tgctgtcggt
     1561 gatcgagtgg ggcttgcctg gctcaatcac acctgtggcg cctgcgagtt ttgttgttcg
     1621 aaacgtgaga atctctgcga acaaccgcag tttactggtt ggactgtcga cggtggttat
     1681 gccgaggatg ctcttgctcc ggcatccttt acctatcaca tccccgacga ctttgaagcc
     1741 gctcaggcgg cgccgctgct ttgcgccggc atcatcggtt atcgctgttt gcgcctcaca
     1801 ggaatcaatg acaatgaatg ggacggtggg cgcctgggct tgtacggctt cggcgccgcg
     1861 ggtcatatct cgattcaact ggcgcgtgca cgcggggcgg aggttttcgt ttgcacccgt
     1921 gaccgtgaac gccatcaggc actggcccac gaactgggcg caacctgggt aggtgatgcg
     1981 agtgacgagc cgcccgtcaa actcgatgct gcaatcatct tcgcccctgc gggtgaactg
     2041 gttccggttg cactcaaagc cgttcgcaaa ggcggcacag tggtattagg tgggatccac
     2101 atgagtgtca ttcccgcgtt cgactattcc ctgatctatg gcgaacgagt agtacgcagc
     2161 gtcgccaaca acactcgcgc ggacggccgt gaatttctag ctgaagccgc ccgcattccg
     2221 gtgcgcactc acgtcgaaac ctttactttc gatcaagcca acgaagcact gctggcgttg
     2281 aagaatgatg cgattcgggg agccggagtg ctgctcatcg gttaaacgga ttcatggcag
     2341 agtacaaaca aaacagaggc aatggcgctg agtatctttg acgaccggtc aactc
//
LOCUS       DASUEX010000040         2380 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1042812, whole genome shotgun sequence.
ACCESSION   DASUEX010000040 DASUEX010000000
VERSION     DASUEX010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1042812"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1321
                     /locus_tag="VFV61_00720"
     CDS             <1..1321
                     /locus_tag="VFV61_00720"
                     /inference="COORDINATES: protein motif:HMM:NF036799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899019.1"
                     /translation="GADRENVLCAQTRCVELICEIAGGAATEDAIDVYPNKEPQASIE
                     FDPARVEALTSLRVPESEMERILTGLGFEATNVAAGARRFKRPSWRVDVEREEDLVEE
                     IARHWGYDKMGSELPPTVLSGEYQHDELKLRSLRRVLKGMGFDEAINFSFIDSAYDDA
                     FALIPAFANTEAGFVTLKNPIIDEAVRMRPTLLVGLLTALRHNLNHGVRDVRLCEIGR
                     VFAKSMAGVLPGEREALALLASGGVVQAGRAEAASETDFFDLKGALEAGVAAMNLGPL
                     RFEAATVKHLRAGQAALISLDEIGVIGSLGRLTDSLAGAFKFRQPVYVAELDLSQLLA
                     SPALAVHYRPLPRYPAVVRDVTLLLDRKVSVAELLNTIASENIAECRGAEFVGTYEGK
                     NIPAGKRTVTLRVEYRADDRTLRDEEVEERQRRLIDSLVQKYSAQLH"
     gene            1420..1701
                     /locus_tag="VFV61_00725"
     CDS             1420..1701
                     /locus_tag="VFV61_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899020.1"
                     /translation="MVGLSGLEKFSHLEDKIYRTIELTKSLRLEKESLEKELSLMRRG
                     KGSPVNGETERLERQVEQLLAERDMIRMKVEAMLDAVAALDPEMAEELR"
     gene            1698..2087
                     /locus_tag="VFV61_00730"
     CDS             1698..2087
                     /locus_tag="VFV61_00730"
                     /inference="COORDINATES: protein motif:HMM:NF017016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEX4899021.1"
                     /translation="MSAGIGQSLGQGSMDSTSHTPTIRVEIYNQTYNIRSDGDTEYII
                     QLAEFVDSRMREISSGTLTVDSLKVAILAALHVADELHRLKNLHEQADAQLATRSAEC
                     AEMLDSLLKARTSVEQTQGGPGKMSLG"
BASE COUNT          523 a          598 c          720 g          539 t
ORIGIN      
        1 cggtgccgat cgcgagaacg tcctatgcgc gcagacgcgg tgtgtcgagt tgatctgcga
       61 aattgcgggc ggtgctgcca ccgaggatgc catagatgtg tatcccaata aggagccgca
      121 ggccagcatt gagtttgatc ccgcgcgagt tgaagcgctg acttcgctgc gagttccgga
      181 atccgagatg gagcgcattc tgactggtct ggggtttgag gccacaaacg ttgccgcagg
      241 cgcgagacga ttcaagcgac cgagttggcg cgttgatgtt gagcgcgagg aagatctggt
      301 ggaggaaatc gcgcgccatt ggggttacga taaaatggga tcggagctgc cccccacggt
      361 tctctcgggc gaatatcagc acgacgagtt gaagctgcga tcgctgcgcc gcgtactgaa
      421 gggaatgggt tttgacgagg caatcaattt tagttttatc gactccgcct acgatgatgc
      481 cttcgctttg attccggctt tcgcgaatac cgaagccggc ttcgtgaccc taaagaaccc
      541 gattattgac gaggcagtgc gcatgcggcc aactctcctt gtcggattgc tgacagcact
      601 gcgtcacaat cttaatcatg gcgtgcgaga tgttcgcctg tgtgaaattg gccgtgtttt
      661 tgcaaaatca atggcgggtg tattgcccgg tgagcgtgag gctctggcgc tgctcgcaag
      721 cggcggcgtc gtacaagcag gacgtgcaga ggctgctagc gaaactgact tttttgattt
      781 gaaaggggcg ctggaggccg gcgtcgccgc catgaatctt ggtccgctgc gattcgaagc
      841 ggccacggtc aaacacctgc gcgctgggca agcggccctg attagtttag acgagatcgg
      901 ggttatcggt tccctgggtc gactgacgga ctcgttagca ggcgctttta agtttcgcca
      961 gcctgtctac gtggcggaac tcgatttgtc gcaattgctg gcgtcgccgg cgttggctgt
     1021 tcattaccgg cccttgccac gctatcccgc agtcgtgcgc gatgtaacgt tgttactcga
     1081 tcgcaaggtt tccgtggcgg agctcctgaa tacgatcgct tccgagaata tcgcagaatg
     1141 ccgcggcgcc gaattcgttg gcacttatga agggaaaaac attcctgccg gaaagcgaac
     1201 cgtgacgctg cgcgtggaat atcgcgccga cgatcggacg ttgcgcgacg aggaggttga
     1261 ggagcggcag cgccgcttga ttgattcgtt ggtgcaaaag tatagtgcgc aactgcatta
     1321 agaaataaat cgtccgtgat tgcgacttga gtcttgcgct tgtgaccgcg aagccgcata
     1381 atcggacgcg ctcgggtttt gtatcgttgg gagagactga tggtcggctt atccggatta
     1441 gaaaagttct cacatctcga agacaagatt tatcgcacca tcgagttgac gaagtcgctg
     1501 cgtctggaga aggagagcct ggaaaaggaa ctctcgctga tgcggcgcgg gaagggcagt
     1561 ccggtaaacg gcgagaccga acgcctcgaa cgacaagtag aacaactttt ggctgaaaga
     1621 gacatgatcc gcatgaaagt ggaagccatg ctagacgccg tcgctgctct cgatccggaa
     1681 atggcggagg agcttagatg agcgctggaa tcggtcaatc cctcggacaa ggcagtatgg
     1741 atagcacctc ccacacccct accattcgcg tcgagattta taaccaaacc tacaatattc
     1801 gttctgatgg cgacactgag tacattattc agttggcaga attcgtcgac agccggatgc
     1861 gcgagatttc ttccggcacc ctcacggtag attccctcaa ggttgccatt ctggcggccc
     1921 tgcatgttgc cgatgagctt catcgcctga agaatctgca tgaacaggcc gacgcgcaac
     1981 ttgcgacgcg cagtgcagaa tgcgccgaga tgctcgatag tcttctgaaa gctcgcacca
     2041 gcgttgagca aacccaaggc ggacccggca agatgtcgtt gggttgataa aggtattgga
     2101 accttcctgc gtgcggtacc ccagtcctcc aggccgaccg gaattcgagt catagatcaa
     2161 cacgcgctag acaattagag gaagcaattt ctcattttgt gttatgtatg taaatctgtg
     2221 gctcggattt gtctcctatg ctagtatcgc tgcggcggag ggaagtaatt tctgcggggt
     2281 tcgtcaccga gttacgatga ttgagccaac gccggttgag aagggagacc gacgcctctt
     2341 gccagtgcag gtcttcatcg ttaagaactg ccagaggctg
//
LOCUS       DASUEX010000041         3871 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_584855, whole genome shotgun sequence.
ACCESSION   DASUEX010000041 DASUEX010000000
VERSION     DASUEX010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3871
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_584855"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="VFV61_00735"
     CDS             complement(<1..210)
                     /locus_tag="VFV61_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899022.1"
                     /translation="MIKKSTFLKLKLREASFITRGLLSSRHPVLAHLIPMRRCNLACG
                     YCNEYDHSSSPVPLEVMKERIDKLAE"
     gene            complement(368..1060)
                     /locus_tag="VFV61_00740"
     CDS             complement(368..1060)
                     /locus_tag="VFV61_00740"
                     /inference="COORDINATES: protein motif:HMM:NF015510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HEX4899023.1"
                     /translation="MKRFGLLMVVMLVLAAALAGSRTPANGQSAGLVSSVLNKMERNR
                     QSLKSLRSGISMEKYNSQLRDSEKFNGVVSYVPGAGREASVRIEWRSPQHEILTVTNG
                     KFVLFRPRLNMAYVGNSKSKNNKAGGVMEMIYMSRAQLESRFQPVQEVREETLWGNVS
                     TIHLKLVPKGNASFKYAEIWVDNSGMPVQTKIVEKNDDATTMRLSGLEKNAKISSDDF
                     SQKLDGVRIVKS"
     gene            complement(1137..1466)
                     /locus_tag="VFV61_00745"
     CDS             complement(1137..1466)
                     /locus_tag="VFV61_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899024.1"
                     /translation="MDTIKKIFLWTYERNTWQWDTLCALILVFIFLTPKSWFSVSERQ
                     VGLGHQSPVPATVFVGAEVVEKQDDKSLLRNHLRVLTGRENAEVGAIRKVVDKEGRTV
                     GYQVDIR"
     gene            complement(1575..2267)
                     /locus_tag="VFV61_00750"
     CDS             complement(1575..2267)
                     /locus_tag="VFV61_00750"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4899025.1"
                     /translation="MRTILKASDLVKVYRVGKVDVPALRGVSFEVQEGEFVAIMGPSG
                     CGKSTMLHLLGGLLTPTSGQIVIDGEDLTAASDAARTDIRRRKIGFVFQRFNLFPTLT
                     AEGNLRLAEKIHGNGGDDPARRREVLKLLGLEDKLHHKPLELSGGEQQRVALARAVVT
                     RPAIVLADEPTGNLDSENSALVLNMFQELNYRFNQTIVMITHNPEAAAMCRRIVQMRD
                     GHIVAPAASVRM"
     gene            complement(2340..3344)
                     /locus_tag="VFV61_00755"
     CDS             complement(2340..3344)
                     /locus_tag="VFV61_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899026.1"
                     /translation="MFAIAVSGCGLFDTHKKVQVPPLLNPLADASTNQLMGEVNRLAA
                     VNSVHGKVDILFEDTSFAEQGVADKYRQADGTITVQRPGKIYLIIQVPFIATDIAQMT
                     SDGQSFRVAVLQGEERFKRFVKGTNNAVYEKLDMDGAGNEGGKKKKTMSGMDTVNALS
                     NLRPQHLTEAFMISPIQPSRDTGLIYSKSEFFQEETDTRPQAKKNSRVVRGYFLLEEM
                     SAPTSGESRLLRRFWFDRVAGIRLARIQTFDERANLITDVSYLDDKGFGQNSVKLPAR
                     IDITRPHDHYRLSITYQSPASVDIDREFRSEAFVLENRWKLPEVDLDSRRAKPAPINQ
                     "
     gene            complement(3543..>3871)
                     /locus_tag="VFV61_00760"
     CDS             complement(3543..>3871)
                     /locus_tag="VFV61_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899027.1"
                     /translation="VSLILVKIPADYFGVSPRSKFLAHESYPVRMLALIGKNVAGALL
                     VVLGIFLSLPGVPGQGVLTILLGVMLLDLPGLRSFERWIVSSPKVFKAVNNLRQRFDK
                     PPLILD"
BASE COUNT          913 a         1047 c          983 g          928 t
ORIGIN      
        1 ctcagctagt ttatcgatac gctctttcat cacctccagc ggtacggggg atgatgaatg
       61 gtcatactcg ttgcaatagc cacaggcgag attgcagcgg cgcataggaa tcagatgcgc
      121 cagcaccgga tggcggcttg aaagtagtcc acgagtaata aagctcgcct cgcgcagctt
      181 gagtttaaga aaggtgctct tcttgatcat ttagctaact tcgtccggag tggccggacc
      241 ctaccaatcc ttttaataga ccacgaagaa taggcgaccg ctccgaggaa cgcaagacgg
      301 cctataaacc aaaagggctc tggtcaaact gccagagccc tccaggcttt tgtaattgaa
      361 ctcgcgttta gctctttacg attctgactc cgtccagctt ttggctgaag tcgtctgacg
      421 agattttggc gttcttctcc agccccgaca gccgcatcgt cgtagcatcg tcgttctttt
      481 ccacgatttt cgtctgtact ggcatgcctg agttgtcaac ccagatctcg gcatatttga
      541 aactggcatt gccctttgga acgagtttca agtgaattgt gctgacgttg ccccacaggg
      601 tctcttcgcg tacctcttgt actggctgga aacgcgactc gagttgcgcg cgcgacatgt
      661 aaatcatttc catcacgccg ccggctttat tattttttga ttttgaattt cccacgtaag
      721 ccatgttcag acggggtcgg aacaagacaa acttgccgtt agtcacggtt aggatttcat
      781 gctggggact gcgccattcg atgcgcaccg aagcctcccg gccagctccc ggtacgtagg
      841 acaccacgcc attaaacttt tccgaatcgc gcagctgcga gttgtatttc tccatgctga
      901 tgcctgaacg cagggacttc aggctctgtc gattgcgctc catcttgttc agcaccgaac
      961 tcaccagacc cgcgctctgg ccattagcgg gtgtgcgcga gccggcaagg gccgccgcga
     1021 gcaccagcat cacaaccatc aataaaccaa atctcttcat atttgctcct cgaattatta
     1081 ccaaaccgac gaccctctgg ctgagggtaa aaaaacccag cgattatagg gtaaggtcaa
     1141 cggatgtcaa cctgatatcc aaccgttctg ccttctttat ctaccacctt acgaattgct
     1201 cccacctcgg cattttccct tcccgtcagg acccttaaat gatttcgcaa aagtgactta
     1261 tcgtcttgtt tttcaacaac ttcagcaccc acaaagacgg tagcgggcac tgggctttga
     1321 tgccccaaac cgacctgccg ttcgctcacc gaaaaccagc ttttgggggt caaaaaaata
     1381 aagaccagaa tcagcgcgca cagggtgtcc cactgccaag tattgcgctc gtaagtccaa
     1441 agaaagattt tcttgattgt atccacgaaa acgaactccc gggcttggac tgccggatga
     1501 atggcaaaag gcgcgccttg cgaacagaag gctctcaaat tctaacttgt gtcgtttccc
     1561 ctgtaaatcg cctattacat acgcacgcta gcggctggag caacaatgtg accgtcgcgc
     1621 atttgtacta ttcgtcggca cattgctgca gcttcgggat tgtgagtaat catgacgata
     1681 gtctgattga agcggtagtt caattcctga aacatgttga ggacgagggc ggagttctct
     1741 gaatcaaggt ttccggtggg ctcatccgcg agcacaatcg ccggccgtgt cacaacggcc
     1801 cgggcaagag cgactcgctg ttgttcacca ccggagagtt cgagcggttt gtgatgaagc
     1861 ttatcttcca ggcccaggag tttcagtact tcccggcgtc gcgccggatc gtcgccgcca
     1921 ttgccatgga tcttctctgc caggcgcagg tttccctccg ccgttagggt gggaaagagg
     1981 ttgaagcgct gaaaaacaaa gccgattttt ctgcgtctaa tgtcagttcg ggccgcatct
     2041 gaagcggcag taagatcttc gccgtcgatc acaatctggc cgctggttgg cgttaataga
     2101 ccgccgagca agtgcagcat cgtcgacttg ccgcaacccg aaggacccat gatggcaaca
     2161 aactcgccct cctggacttc aaaagacacg ccgcgaaggg ccggcacatc aaccttgcca
     2221 acgcggtaca ccttaaccag atccgaggct tttagaatcg ttctcatcgg gaattagata
     2281 agcaaacgcg ccgaatggta atacatcggc tcgcctaaca cctagtaagc caaactgagc
     2341 tactgattga ttggggctgg ctttgcgcgg cgggagtcga ggtcgacttc gggaagcttc
     2401 cacctgtttt ccaggacaaa ggcttccgat cggaattcgc gatcgatgtc tactgacgct
     2461 ggcgactggt aggtaatgct taaacgataa tggtcgtgcg gccgcgtgat gtcgatacgt
     2521 gccggaagtt tcacgctgtt ctgcccaaat cctttgtcgt cgagatacga gacatcggtg
     2581 atcagattcg cgcgttcgtc aaacgtctga atccgtgcca gccgaattcc cgcgacgcgg
     2641 tcaaaccaaa accgacgcaa gagccgactc tcgccactcg ttggcgctga catttcttcc
     2701 aacaggaagt aaccgcgcac gacacgcgag ttctttttgg cttgtggtcg agtgtcggtc
     2761 tcttcctgaa agaactcgct cttggagtaa atcagccctg tgtctctgga tggttgaatt
     2821 gggctaatca tgaacgcttc ggtcaaatgt tgcggccgaa gattcgagag ggcattcaca
     2881 gtatccatgc cactcatggt tttcttcttc ttgccgcctt cattcccggc gccatccata
     2941 tcgagcttct cgtagacggc attgttggtg cccttaacga agcgtttgaa cctctcttcc
     3001 ccttgcagta ccgccacgcg aaacgactgg ccatcggaag tcatctgcgc aatgtcagtc
     3061 gcgataaatg gcacttgaat gatcagatag atcttgccgg gacgttgaac tgtaattgtg
     3121 ccatcggcct ggcggtattt gtcggcgacg ccctgctccg cgaaagatgt gtcttcgaag
     3181 aggatgtcca ctttgccgtg aaccgaattg acggcggcaa gccgattaac ttctcccatt
     3241 agttgattcg tgctggcgtc ggccaaagga ttaagcagag gtggtacctg cacctttttg
     3301 tgggtatcaa aaaggccgca accgctgact gcaatcgcaa acaccagaag tacgagaatg
     3361 agaggtaatc gagatcttgg agctttgcag gaggactggg ggacgaggga tgtaatgggc
     3421 atcaacgaag gtttaagaaa caaatcgtaa ttcgcctcga tatcgtccag agacttagcg
     3481 ggttcaacct agcaaagaga cttttcattg tcaacgcact gaacttttgc cgttgcatgg
     3541 tcttagtcga gtatcagtgg tggcttatca aatcgttgtc gcaggttgtt taccgcttta
     3601 aagaccttcg gtgaacttac gatccaacgc tcaaaactcc gcaggccagg caagtcgagc
     3661 aacatgaccc ccagcagaat tgttaagact ccttgccccg gaactcccgg caacgagaga
     3721 aagatgccga gaacaaccag cagagctcct gcaacgttct tgccaattag cgcgagcatg
     3781 cgaaccgggt aagactcatg tgcgaggaac ttgctgcgcg gcgacacccc aaagtaatca
     3841 gcgggaattt tgaccagaat cagtgacacc a
//
LOCUS       DASUEX010000042         6058 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_928734, whole genome shotgun sequence.
ACCESSION   DASUEX010000042 DASUEX010000000
VERSION     DASUEX010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6058
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_928734"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..415)
                     /locus_tag="VFV61_00765"
                     /pseudo
     CDS             complement(83..415)
                     /locus_tag="VFV61_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007292390.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(421..675)
                     /locus_tag="VFV61_00770"
     CDS             complement(421..675)
                     /locus_tag="VFV61_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899028.1"
                     /translation="METIASEATISNSRNISTPSPSSLRKVKRARDRLRRQPNARRGS
                     QVARPGIKMHSNSPSAWSARNARDDLKMVAILMQSTEALG"
     gene            715..942
                     /locus_tag="VFV61_00775"
     CDS             715..942
                     /locus_tag="VFV61_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899029.1"
                     /translation="MASRREVTPALVVALAEYAELPLAPDREKVITSTLETWIRDANE
                     LSRKMSSAKYWELAPATIFSHANEAEERELK"
     gene            939..2360
                     /locus_tag="VFV61_00780"
     CDS             939..2360
                     /locus_tag="VFV61_00780"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX4899030.1"
                     /translation="MRKATRELLELSLAQAARLVKSKEISPVDLVDASLERIKSVDDR
                     LSAYITIFEEEARKVAKAAETMIVAGHDLGPLHGVPIGLKDNLAVKGFRTTAGSKVLK
                     DWVPDHDATVTERLRRAGAIFVGKLNMHEFAWGGTSDNPHYGAVRNPWNTERFPAGSS
                     GGSGAAVAARTCYGAIGTDTGGSIRLPSAINGIVGIRPTYGRVSNYGIIPLAWSMDTA
                     GPMTRTVEDCAIMFNAIAGHDPKDPSASRAPVADYAEDLKRGVMGLRIGVVPGYFFHH
                     LQPPVHDAVKGGLKTLEGLGAAVVDVNIENIHGNISAQLTIESAEPSAYHQRWLREQP
                     ESYGADVRTLLEIGEMLLATHYLQAQRYRTLLRREFLEAFKKVDVFICPTLPFTATKV
                     GEMKVVIENGVEENMLSAIMQFTGVPSLTGLPSLNVPCGFDQDGLPVGMQIIGKPFDE
                     ATLFRIGAAFQSATDFHKRAPDI"
     gene            2405..3628
                     /locus_tag="VFV61_00785"
     CDS             2405..3628
                     /locus_tag="VFV61_00785"
                     /inference="COORDINATES: protein motif:HMM:NF024825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX4899031.1"
                     /translation="MSRKFMNGALAALMMAASLGAAAADKKPIVVGSILDETGPLNIY
                     GKAMVDATKLAIKQINENGGVLGRQLKLVSYDAQSDNAKYTLYANQLSLKDKAVVIMG
                     GITSASREAVRPVINRNKTLYFYNEQYEGGVCDKNVFATGIVPSQQVAPTVAWAMKNI
                     GKKFYILAADYNYGHISTDWVKKYVAQEGGQVVGSEFIPLDVAEFSSIITKLQEAKPD
                     VVFSLLVGGNHIAFYRQFAASGLRDKMKIVSATFGLGNEQVVVAPEEAKNIVAVYPYF
                     QEIDTPANMQFVAMWHKEFGKDYAYITDSAVTVWNGWHLWANAVGKTGSTDRAKVIKA
                     LESGISFDGPSGTVTIDGPSHHVYQNTHFARTNDKHGFTVLGGEKKVPPAFEKKMCDL
                     IKKPNQHMQFLPETK"
     gene            3658..4533
                     /locus_tag="VFV61_00790"
     CDS             3658..4533
                     /locus_tag="VFV61_00790"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899032.1"
                     /translation="MDWSQFDWLINLALSMQLSIAVLVIVSIGLAVVFGMMGIINLAH
                     GEFLMLGAYATLACVRAGINLWLSMGIAAFVVGMFGLVVEHLLIRHLYGRLADSMLAT
                     WGLSLIMAQVMVLIFGPSTQGIATPLGSVNIGAYSVSGYSFVLIAAAVVLLAIVYWVF
                     TRTPYGVMARAAMQLPGMAAAVGINPKLINAGTFAFGAALAGAGGALLAPLVGVMPSM
                     GQAYIARAFMTVIVGGPGVISGTASSAVLLGGMENVVSYLSTPFFGQGALLVLAIVLL
                     RVLPTGISGRWQRQL"
     gene            4580..>6058
                     /locus_tag="VFV61_00795"
     CDS             4580..>6058
                     /locus_tag="VFV61_00795"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX4899033.1"
                     /translation="MNKNKTRQGFAVEAMGTVAVTAAMAGLAAGISEFQSLQIASWAI
                     YGLLALSLVLVWGHAGIFSFGQGAFFGIGGYAYGVAAINLIGVTSETFSAVAIAVAVA
                     AIAAAFLGYFMFYGKVGDVYVGIITLATTLVLFTFMSSTAGPQYHLGEALLGGYNGMT
                     GVPPLSYGTPGEESNAFSPGELLVITATLAGLVTLGVYGLLRGSFGRVLAGLRENELR
                     TQLLGYDIRFRKLVVFAIGGAIAGLAGALYAAWAMFISPGVFGLQQAAMVVIWVLVGG
                     RTSVAGAFVGVVLVDGLASFLGGSGGNATPIILGAVLIGVVLLLPQGLVPAIQAGLGR
                     FFKETRKDKVTAAAEAVSEKSSRYAGLRVSAGREGGVLEVVDLHKKFGGVTAVEKVTM
                     SFTEKGVHCLIGPNGAGKSTFFNLLVGRYWPTAGTVMFAGKPITRAEAHERVRRGLGI
                     KLQVASIFAGLTTFENVWLSGFGKLHDSPAATERANKILDWLG"
BASE COUNT         1221 a         1613 c         1874 g         1350 t
ORIGIN      
        1 atcggcaaat aaggcctccc taccggctgc catggcgagt ttcggctcgg gcttggcata
       61 ttgcatcggt ggtgtcccaa cgttattcga acggaatcaa ctgattggta gaaacggact
      121 cgtttgactc tggtgctgcg agtgtaagca gtcgatgcaa aggggttcgc tcgaacatgg
      181 tcaggctcaa tatctgtagg atttcataga ggctcgcgga gagcttgagc ctcttcttga
      241 cgatggcgac gagcacgtag accgagacgg cgttccagat ttgcgttttg accgcattct
      301 cggtggtgcc gaaaaaggcc ttgatacgaa gatgctgttc gatccatttg aagaacaact
      361 cgatttgcca acgcagccgg tagagcttgg ttatcgtgag cgcaacgctt tggtcgccga
      421 tcatcccagt gcttcggtcg actgcatcag tatggcgacc atcttcaagt cgtccctcgc
      481 attcctggcg ctccaggcgc tcgggctgtt gctgtgcatc ttgattcccg gcctcgccac
      541 ctggctgccg cgccgggcgt ttggctgacg gcgcagacga tcccgcgctc tcttcacttt
      601 acgtaaactt gacggtgatg gcgtgctgat atttcgtgaa tttgaaattg ttgcttcgct
      661 cgcgatggtt tccattcaac aaacattcgt ctgacaaatg aacaaggggg tcttatggct
      721 agccgcagag aagtaacgcc ggccttggtt gttgcgctgg ctgagtatgc ggaactaccg
      781 cttgcgccgg accgcgaaaa agtcatcact tcgacgctgg aaacctggat cagggatgct
      841 aacgaactaa gccggaaaat gagctctgca aagtattggg agttggcacc cgcaacaatc
      901 ttcagccacg ccaatgaagc cgaagaaagg gaactgaaat gagaaaggct accagggaac
      961 ttctggaact ctccctcgcg caggctgcgc gcctcgtcaa atccaaggaa atttcccctg
     1021 tagatctggt cgacgccagt cttgaacgta tcaaatctgt cgatgaccgg ctatcggctt
     1081 acatcaccat attcgaagaa gaggcccgca aggtcgccaa ggcggcagaa accatgattg
     1141 ttgccggcca cgatcttggc cccctgcatg gcgtgccaat cgggctaaag gacaatctgg
     1201 cagtcaaagg gtttagaacg accgccggga gcaaggtcct caaggactgg gttccggacc
     1261 acgatgcaac cgttaccgag aggctgcgtc gtgccggtgc aatctttgtc gggaagttga
     1321 acatgcacga gtttgcctgg ggcgggacct ccgacaaccc gcactatggc gccgtgcgca
     1381 acccctggaa cacggaacgc ttcccggcgg gatccagcgg cgggtctggt gcagccgttg
     1441 cggcaagaac ttgttacggt gccatcggca ccgacaccgg cggatcgatt cgactgccct
     1501 cggcgatcaa cggtatcgtc ggcatacggc cgacgtacgg acgcgtgagc aattacggga
     1561 tcattcccct tgcctggagc atggataccg ctggcccgat gacgcggacg gtcgaagact
     1621 gcgcgatcat gttcaatgcg atcgcgggcc acgatcccaa ggatccgagc gcatcgaggg
     1681 cgccggttgc ggactacgcg gaggacttga aacgcggtgt catgggcctg cgaatcggcg
     1741 tggtgcccgg gtactttttc catcatctgc agccacccgt gcacgatgcg gtgaagggcg
     1801 ggctgaaaac gctggaaggg ctcggggccg cagttgtcga cgtgaatatt gagaacattc
     1861 acggcaacat ttccgctcaa cttaccatcg agtctgccga gccgagtgca tatcatcaac
     1921 gttggctgcg cgaacagccg gaaagttatg gtgccgacgt tcgaacgctg cttgaaatcg
     1981 gcgaaatgct gctcgcgacg cactacctgc aagcgcagcg ctatcggacg ctgttgcgcc
     2041 gcgagtttct cgaggctttc aagaaggttg acgttttcat ttgcccgacg ctcccgttta
     2101 cggccacgaa ggtgggtgaa atgaaggtcg tcattgaaaa cggggtagag gaaaatatgt
     2161 tgtctgctat catgcagttc acaggcgtgc cgagcttgac gggattgcct tcgttgaatg
     2221 tgccatgcgg cttcgatcaa gatggtttgc ctgtgggaat gcaaattatc ggcaagccgt
     2281 ttgacgaggc aacgctgttc aggattggtg ctgcgtttca gtcggctacc gacttccata
     2341 aacgcgcccc tgatatctga agttgtcggg cgttattcgt aacaccaaac aaggagaggg
     2401 aacaatgtca cgcaaattca tgaacggcgc tttggcggca ttaatgatgg cagcttcgct
     2461 gggagcggct gcagccgaca agaagccgat cgttgtggga agcattcttg atgagaccgg
     2521 ccccctgaac atctatggca aggccatggt agacgccacc aagctggcaa ttaaacaaat
     2581 caacgagaac ggcggcgtgc tcgggcgtca gctgaagctg gtttcttacg atgcccagtc
     2641 cgacaacgcc aagtacactc tgtacgccaa tcagctttcg ctgaaggaca aggcggttgt
     2701 gatcatgggc ggcattacca gcgcctcgcg ggaagcggtg cgtccggtca tcaatcgcaa
     2761 taaaaccctg tacttctaca acgagcagta tgagggcgga gtctgcgaca agaacgtctt
     2821 tgccaccggc atcgttcctt cgcagcaagt ggcgcccacg gttgcctggg caatgaagaa
     2881 catcggcaag aagttctaca tcctcgccgc cgactacaac tatggtcaca tctcgactga
     2941 ctgggtcaag aaatacgtcg cccaggaggg tggccaggtg gtcgggagcg agttcattcc
     3001 gctggatgtt gcggagttca gttccataat caccaagctc caggaagcca agccggacgt
     3061 cgtcttttcg ctcctggtcg gcggcaacca tatcgctttc tatcgccagt tcgcggcttc
     3121 cggtttgcgc gacaagatga agatcgtgtc cgcaaccttc ggcctcggca atgagcaggt
     3181 ggtggtcgct cccgaggagg ccaagaatat tgttgccgtg tacccgtact tccaggaaat
     3241 cgatacgcca gccaatatgc agtttgtcgc gatgtggcac aaggaattcg gcaaggacta
     3301 tgcgtacatc accgacagcg cggtcacggt ctggaacggc tggcacctgt gggcgaatgc
     3361 agtggggaag acgggaagca ctgatcgcgc gaaagtcatc aaggcgctcg aatccggtat
     3421 cagctttgat ggcccgtcgg ggacggtcac catcgacggt ccttcgcacc acgtatacca
     3481 gaacacgcac ttcgcgcgga ccaacgacaa gcacggattc acggtgcttg gcggtgagaa
     3541 aaaggtaccc ccggcattcg aaaagaagat gtgcgatctg atcaagaagc ccaatcagca
     3601 catgcagttc ctgccagaga cgaagtaaat ctctgaaggt tagcgctagc ggaaattatg
     3661 gattggagcc agtttgactg gctgatcaac ctcgcgctgt cgatgcaatt gagcatcgca
     3721 gtactcgtga tcgtgtccat cgggctggcg gttgttttcg gcatgatggg catcatcaac
     3781 ctggcgcacg gagaattctt gatgctcggc gcgtatgcga ccttggcgtg cgtgcgcgcc
     3841 ggcatcaacc tctggctatc gatgggtatt gccgccttcg tcgtcgggat gttcggcctt
     3901 gtcgtagagc acttgttgat ccggcatctt tatggccgtc ttgctgacag catgctcgcg
     3961 acctggggtc tcagtctgat catggcgcaa gtgatggtgc tgatctttgg cccgagcacc
     4021 cagggaatcg ccacacctct cggaagtgtc aacatcgggg cgtactcggt gtccggttat
     4081 tcatttgtcc tgatcgctgc tgcagtcgtt cttctcgcaa ttgtttattg ggtcttcaca
     4141 cgcacgccgt acggagtcat ggcgcgcgcc gcgatgcagc tgccggggat ggcggcagcg
     4201 gtaggaatca atcccaagct gatcaacgcc ggtacctttg ctttcggcgc cgcattggcc
     4261 ggtgccgggg gggcactcct cgcgccgttg gtcggggtca tgccgagcat gggccaggcc
     4321 tacatagccc gcgcgttcat gactgtgatt gtcggcggcc ctggagtcat cagcggcact
     4381 gcaagttccg cggtgcttct gggggggatg gagaacgtgg tgtcgtatct ctcaacacca
     4441 tttttcggtc agggcgctct cctggttctc gccatcgtgt tgctgcgcgt gctgccgacg
     4501 ggcatatccg ggcggtggca gcgccagctt taagacctcg acaaaaatgt gtgcgctact
     4561 gcggtagcgc aaggcgatga tgaacaaaaa caagactcgt cagggatttg ctgtcgaggc
     4621 tatggggacg gtcgccgtga cggcggcaat ggccggtctg gcagcgggaa tcagcgagtt
     4681 ccaatcgctt cagatcgcga gctgggcgat ctacgggctt cttgcactca gtctcgtgct
     4741 ggtttggggg cacgcgggaa tcttcagctt cgggcagggg gccttcttcg ggatcggagg
     4801 ttatgcctac ggcgtcgctg caatcaacct tatcggcgtg accagtgaga cgttctctgc
     4861 cgttgcgata gcggttgcgg ttgctgcgat tgcggcggcc tttcttggct acttcatgtt
     4921 ttacggcaag gtgggcgatg tctatgtagg catcattacc ctggcaacca ctttggtctt
     4981 gttcacgttc atgtcgagca ccgccgggcc gcagtatcac ctcggcgagg ccctgctcgg
     5041 cggttacaac gggatgaccg gtgttccgcc attaagttat ggaacgccag gagaggaatc
     5101 gaacgcgttc tctccaggcg agttgttggt catcaccgcg actctcgccg gcttggtgac
     5161 attgggtgtt tatggcttgc tgagaggctc ttttgggcgc gttcttgcag gtctgcggga
     5221 aaatgaactg cggacccagt tgctcggcta cgatattcga ttcaggaagc tggtggtctt
     5281 tgccatcggc ggtgcgatcg ccggcttggc cggggccctt tatgcagcct gggcgatgtt
     5341 catcagccca ggagtgtttg gtttgcagca ggccgccatg gttgtgatct gggtgctggt
     5401 cggagggcgg acgtcggtgg caggcgcgtt tgttggcgtg gtgttggttg acgggttggc
     5461 ttctttcctg ggcgggagcg gcggcaatgc gactccaatc attttgggtg ccgtcttgat
     5521 tggggtagtg ctgttgctgc ctcaaggatt ggtcccggcg atccaggccg gcttgggtcg
     5581 ctttttcaaa gagaccagga aagacaaagt cactgcagcg gctgaagccg tttccgaaaa
     5641 gtcttcccga tatgcagggt tgcgggtgtc cgcaggccgg gagggcgggg tgctggaagt
     5701 cgtcgatctg cataagaagt tcgggggggt gacggctgtc gagaaagtca cgatgtcctt
     5761 caccgagaag ggtgtgcatt gcctcatcgg tccgaacggc gcgggcaaga gcacgttctt
     5821 caacctgctg gtggggcggt actggccgac ggcgggtacc gtcatgttcg cggggaagcc
     5881 cattacccgc gccgaggcgc acgaacgcgt aagacgtgga ctcggaatca agctccaggt
     5941 cgccagtatt tttgccggac tgaccacttt cgaaaacgtg tggctttccg gctttggaaa
     6001 gctacacgat tcgcccgcgg ccaccgagcg tgccaacaaa atccttgact ggttgggt
//
LOCUS       DASUEX010000043         2191 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1609850, whole genome shotgun sequence.
ACCESSION   DASUEX010000043 DASUEX010000000
VERSION     DASUEX010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1609850"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1038)
                     /gene="hslU"
                     /locus_tag="VFV61_00800"
     CDS             complement(<1..1038)
                     /gene="hslU"
                     /locus_tag="VFV61_00800"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: protein motif:HMM:NF003544.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HEX4899034.1"
                     /translation="MDEEARLDEMSPRQIVTELDKHVVGQRNAKRAVAIALRNRVRRQ
                     KLAPEMAVDVMPKNIIMIGSTGVGKTEIARRLARMSNSPFLKVEASKFTEVGYVGRDV
                     ESMIRDLTEISIEMTRQDKSEEVAEKAELNVEERILDLLLPPPRSSFYDFDADTSDEQ
                     STELDSPARDEQLQKTREKLRTQLRSGKLDNRLVELEVREKNFPVIELAGPQGVEEMG
                     INMKDMLGNLFQGRTKRRKMRVDEAMEYLMQEEEERLIDMDTVARAALERVESSGIIF
                     LDEIDKIAGREGGHGPDVSREGVQRDILPIVEGTTVNTRYGMVRTDHILFIAAGADVL
                     RALVHLEGEVGH"
     gene            complement(1126..1671)
                     /gene="hslV"
                     /locus_tag="VFV61_00805"
     CDS             complement(1126..1671)
                     /gene="hslV"
                     /locus_tag="VFV61_00805"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015365078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HEX4899035.1"
                     /translation="MHEEKNRVRSTTVLLVRKDAKVALAGDGQVTMGETVMKASARKV
                     RRLYSDQILAGFAGATGDAFALLTRFESKLEQYHGNLERAAIELSKEWRTDKMLRHLE
                     ALLVVADKKASFLLSGNGDVIAPDEGVLAIGSGGSYALAAARALIKNTDLSAREIAVE
                     SLRIAGEICIYSNQNIIVEEL"
     gene            1762..>2191
                     /locus_tag="VFV61_00810"
     CDS             1762..>2191
                     /locus_tag="VFV61_00810"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEX4899036.1"
                     /translation="MIAAGGIIPEGMMQTRPRIGITMRIEHTTNRFYLGRDYSEAVEA
                     ASGSPVHISLIPNADYIDSVVAHVDGILLPGSDTDVDPLRYGQQPHPRLGTVQTIKDE
                     TDLLVIEAAERRGIPVFAICFGMQVLNVSRGGTLIQDLNSQ"
BASE COUNT          448 a          651 c          583 g          509 t
ORIGIN      
        1 gtggccaact tcgccttcaa ggtgaacgag ggcacggaga acatcggcgc ccgccgcgat
       61 gaacaggatg tggtcggtgc gcaccatgcc ataacgcgtg ttcaccgtcg tgccttcgac
      121 gatgggcagg atgtcgcgct gcacgccttc gcggctgacg tcggggccgt ggccgccttc
      181 gcgcccggcg atcttgtcga tctcgtcgag gaagatgatg cctgaagatt cgacccgctc
      241 gagagcggcc cgcgcgacgg tatccatgtc gatgaggcgt tcttcctctt cctgcatcag
      301 gtattccatc gcctcatcaa cgcgcatttt gcgtcgcttg gtgcgaccct gaaagagatt
      361 gcccaacatg tctttcatgt taatgcccat ctcttcaacg ccctgtggac cagcgagctc
      421 gatcacggga aagtttttct ctctgacttc gagctcgacg agccggttgt caagcttgcc
      481 gctgcgtaat tgggtgcgca gcttctcgcg tgttttttga agctgctcgt cccgtgcggg
      541 gctatcgagc tcggttgatt gttcgtcact ggtgtcagcg tcgaaatcat aaaaactcga
      601 acgtggcggc ggcagcagta agtcgagaat gcgttcttcg acgttcagct cagccttttc
      661 cgcgacttct tcgctcttgt cctgacgcgt catctcgatc gagatctcag tgagatcgcg
      721 gatcatgctc tcgacgtcgc ggccgacata accgacttca gtaaacttcg acgcttcaac
      781 cttcagaaac ggcgagttgg acatgcgcgc gagacggcgt gcgatctccg ttttaccaac
      841 accggtcgag ccgatcatga tgatgttctt cggcatcacg tcgactgcca tttccggcgc
      901 gagcttctgc cttcgcacac gattgcgcag ggcgatagcc acggcgcgtt ttgcattacg
      961 ctgaccgacg acgtgcttat cgagctcggt cacgatctga cgaggcgaca tctcgtcaag
     1021 ccgcgcctct tcatccactt ctccagccag ataaataacc attgctttaa actaattcct
     1081 taagtcccag gatcttgtga gtcttgcaaa tccggtaatc ctgtctcaaa gctcctctac
     1141 gataatgttc tgattagagt aaatacagat ctcacctgcg atgcgaagtg actccaccgc
     1201 aatctcgcgc gcagagagat ccgtattctt gatcagcgca cgcgcggccg cgagggcata
     1261 ggagccaccc gagccgatcg ccagcacacc ttcatcaggc gcgatcacat cgccattgcc
     1321 tgacaaaaga aacgaagcct tcttatcagc cacgaccagc agcgcctcga gatgtcgcaa
     1381 catcttgtca gtgcgccatt ctttgctgag ctcgatcgcc gcgcgctcga gattgccgtg
     1441 atattgctcc agtttgcttt caaaacgcgt caggagggcg aatgcgtcgc ccgtcgcccc
     1501 ggcaaagcca gccagaatct ggtcgctata aagccgccgc accttcctgg cgctggcttt
     1561 cataacagtc tcgcccatcg tcacctgccc gtctccggca agcgctacct tagcgtcttt
     1621 cctgacgagc agcactgtgg tgctacgaac ccgatttttt tcttcgtgca tttttcttta
     1681 acgagactat aggaactcgt ttcaatgtaa gtcaaggacg ccttccggcg ctgtagtgat
     1741 gtagttttga gtagataggt tatgatagcc gcgggaggaa ttattcctga gggaatgatg
     1801 caaacacgac cgcgcatcgg catcacgatg cgcattgaac acaccacgaa tcgtttttat
     1861 ctcgggcgcg attacagcga ggccgtcgag gccgccagcg ggtctccggt acacatcagt
     1921 ctgattccaa acgcagacta catcgactca gtggtcgcac acgtggacgg cattctgctt
     1981 cccggaagtg acactgacgt cgatccgctg cgctatggcc agcagccgca cccgcggctc
     2041 ggcacggtcc agactatcaa agacgaaacc gatctgctgg tgattgaagc cgccgaaagg
     2101 cgtggaatac cggtcttcgc gatctgtttc gggatgcagg tgctgaacgt gtcgcgtggt
     2161 ggaactctga tccaggacct caactcccag a
//
LOCUS       DASUEX010000044         3287 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1501391, whole genome shotgun sequence.
ACCESSION   DASUEX010000044 DASUEX010000000
VERSION     DASUEX010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3287
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1501391"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            117..1355
                     /locus_tag="VFV61_00815"
     CDS             117..1355
                     /locus_tag="VFV61_00815"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX4899037.1"
                     /translation="MKFIETLKLAVAAIWAHKLRSALTLLGMIVGVTAFVVVVSLIAG
                     FNRYVDEKIAGIGAKSFTVQRFNPLEDFKDTDTIAAAQRRNKDLTMDDYDYLKARATL
                     VDKIGARARGTPSEVKRGNEVLEDVFVSGATANCVDIENRDVAEGRFIAEPENEGAAR
                     VAYIGADVATKLFPAGNTIGSEITVRGLPYRVIGVEVAKGTVFGIPQDTFIILPLKTY
                     AVNYGGLTRGRSLYFVATSKTDELFNDAVEESRFLMRARRKLGPNEKDNFGIVTPDAI
                     TGLRDRLFGTIFLVAIFVPGIAMVVGAIVIMNIMLVSVTERTKEIGIRKSLGARKIDI
                     LKQFLVEAVTLALIGGAVGIFLAWVVGQVVTTLIFPTYLSIAAILLALGVSGGAGVAS
                     GLFPAWKAARLDPIEALRAD"
     gene            1356..2612
                     /locus_tag="VFV61_00820"
     CDS             1356..2612
                     /locus_tag="VFV61_00820"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX4899038.1"
                     /translation="MKILRSDIYENLMMALATLRANKLRSFLTVFGVVIGIITVMLIA
                     SIISGIDVSVKREVESFGTRSIYISKYNPGIHIGRMSREERMRKELTYDDAVALQSLP
                     TIELSVPFLDITNNFFGGKLMVSGGGKTSAQVALQGTLPEFEKAGTQVISEGRFFTQT
                     ENDSNQEVCVVGSKVADDFFKIGSPVGQTIKIGTQEFRVVGVLEKREQFLIGGGSDDQ
                     NNVIYVPFTVAQKLKPNADDIYILAVARPGMMEEAKDQVRDMLRVRRQVAFAANDNFG
                     METSESILDNFRSITAGLAIAMVAISSVGLMVGGIGVMNIMLVSVTERTREIGVRKAI
                     GARRSDIMWQFLIEAATLTGLGGIVGLSIGWGLTLLLRLLLPSYVPLWAPVGGLLASV
                     GIGLIFGLWPAWKAARLDPIESLRYE"
     gene            complement(2629..>3287)
                     /locus_tag="VFV61_00825"
     CDS             complement(2629..>3287)
                     /locus_tag="VFV61_00825"
                     /inference="COORDINATES: protein motif:HMM:NF016764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HEX4899039.1"
                     /translation="FFEPSRTFEALRARPRFLVAGIILLVLAAIVTAVLYLRVDMGQY
                     IRERIERSPNAAQVPEAQKEMQVKFGKIVGMVLVPAITPITIAAGAALYLLGVMAMGG
                     AISYKKAVSVWTYSSLPPSVLGSLIAILVLFLKASDSIDPEKMVVTNPGALFGEESSK
                     VLVAALSQFDLLRFYGMFLAAVGLRKVAKISSGQAWGIVIGLWAIAAFLRILLAAVRG
                     "
BASE COUNT          716 a          924 c          940 g          707 t
ORIGIN      
        1 tcttcggtct gtggcccgcg tggaaagcgg cgcggttgaa ccctattgac gccctccgct
       61 gggagtaatc atgataattc tgtaaatcct gtaatcctgt ctataatgca atcacggtga
      121 aattcatcga aactctcaaa cttgctgtag ccgcgatctg ggcgcacaag ctgcgctcgg
      181 cactcacgct gctcggcatg atagtcggtg tgacggcgtt tgtcgtggtc gtctcgctca
      241 tcgcgggctt caatcgttac gtcgacgaga agatcgccgg catcggcgcg aagtcgttta
      301 cggtccaacg cttcaacccc ctcgaggact tcaaagacac tgatacgatt gccgcggccc
      361 agcgacgtaa taaagatctg acgatggacg actacgacta tctgaaagca cgcgcgacgc
      421 tcgtcgacaa gatcggcgca cgggcccgag gcactccgtc ggaagttaag cgcggcaacg
      481 aagtgctgga agacgttttt gtttccggcg ccactgcgaa ctgcgtcgat atcgagaatc
      541 gcgacgtcgc cgaaggacgc ttcatcgccg agcctgagaa cgaaggcgcc gctcgcgtcg
      601 cctacatcgg cgccgacgtc gctaccaagc tctttccggc cgggaatacc atcggctccg
      661 agattaccgt tcgcggactt ccctatcgtg tgatcggtgt cgaagtggcg aaaggaaccg
      721 tgttcggtat cccgcaggac actttcatca tccttccctt gaagacttac gcagtgaact
      781 acggcggact gactcgcgga cgctcgcttt acttcgttgc aacttcgaag acagacgagc
      841 tcttcaacga cgctgtcgag gagtcgcgct tcctgatgcg cgctcgccga aaacttggac
      901 caaacgagaa agacaatttc ggcatcgtca cgcctgatgc gatcacgggg ctgcgcgata
      961 gactcttcgg gaccattttt ctcgtggcca tctttgttcc gggtatcgcg atggtggtcg
     1021 gcgcgatcgt gatcatgaac atcatgctgg tatcggtaac cgaacgcacg aaggagatcg
     1081 gcatcagaaa gtctctgggc gcacgcaaga tcgatattct gaaacagttc ctcgtggaag
     1141 cagtgaccct cgcgctcatc ggtggcgcgg tgggaatatt tttggcgtgg gtcgtcggcc
     1201 aggtagtgac gacgctgatc tttcccacct acctgtcgat agccgcgatc ctcttagcgc
     1261 tcggcgtttc gggcggcgca ggggtagcgt caggactctt cccggcgtgg aaggcagcgc
     1321 ggttggaccc gattgaagcc ttgagggctg actaaatgaa aatcctgcga agcgacatct
     1381 acgaaaacct catgatggcc ctggcaacgt tgcgcgccaa taaactgcgc tcgtttctga
     1441 cggtcttcgg cgtggtgatc ggcattatca ccgtcatgct gatcgcgtcg atcatcagcg
     1501 gcatcgacgt ttccgtaaag cgcgaagtcg aatcgttcgg cactcgctcg atctacatca
     1561 gcaaatacaa tccgggcatt cacatcggcc gcatgtcacg cgaagagcgc atgcgtaaag
     1621 agctgactta tgacgatgcg gttgcgctcc agtctttacc caccatcgag ctgtcagtgc
     1681 cgtttctcga catcactaac aacttctttg gcggcaaact aatggtttca ggcggcggca
     1741 aaacgtccgc acaggtcgcc ctccagggca ccctccccga gtttgaaaaa gcgggaacac
     1801 aggtaatttc cgaaggccgt ttcttcacgc aaacggaaaa cgattcgaac caggaagtct
     1861 gtgtcgtggg ttcgaaagtc gctgacgact tcttcaagat cggctccccg gtcggccaga
     1921 cgatcaagat cggcacgcag gagtttcgtg ttgtcggcgt gctggagaag cgcgaacagt
     1981 ttctcatcgg cggcggcagt gacgatcaga acaacgtgat ctacgtgccg tttacggtgg
     2041 cccaaaagct caaaccaaat gctgacgaca tctacattct cgccgtcgcg cgtccaggca
     2101 tgatggaaga agcaaaagac caggtgcgcg acatgctgcg cgtgcgccgg caagtggcgt
     2161 ttgctgcaaa cgacaatttc ggaatggaaa cgtcggaaag catcctcgat aattttcgtt
     2221 cgattactgc cggtctcgcg atcgcgatgg tggcgatctc ttcagttggg ctgatggtcg
     2281 gcggcatcgg cgtgatgaac atcatgctcg tgtcagtcac tgagcgcacg cgcgagatcg
     2341 gcgtgcgcaa agcgattggc gcgcggcgca gcgacatcat gtggcagttt ttgatcgaag
     2401 ccgcgacgct tacgggactg ggaggaattg tcggactgtc gatcggttgg ggactgacgt
     2461 tgttactgcg gttgttgctc ccgtcttatg tgccactctg ggcgccggtt ggtggtctgc
     2521 tagcgagcgt cggcatcggc ctgatcttcg gcctctggcc cgcgtggaaa gcggcccggc
     2581 tcgacccgat cgaatcactt cgttacgagt agtcaaagta gtgtggcgct aaccgcggac
     2641 ggctgccaaa agaattctta agaaggctgc gatggcccac aatccgatta cgattcccca
     2701 tgcctggcca gacgaaatct tcgcgacttt tcgcaaaccc acggccgcga ggaacattcc
     2761 ataaaacctg agcaggtcaa actggcttaa ggcagccacg agcaccttcg acgactcctc
     2821 gccaaaaagt gctcctggat ttgttaccac cattttttct ggatcgatcg agtcggacgc
     2881 tttgaggaac aggacgagga tcgcgattaa ggatcccaac accgacggtg gtaacgacga
     2941 ataagtccag actgacaccg ccttcttgta gctaatcgca ccgcccatcg ccatcacgcc
     3001 taacaaatac agtgctgcac cggctgcgat ggtgatcgga gttattgccg gcacgagcac
     3061 catgccgaca attttgccga acttaacctg catttccttc tgcgcttcgg gcacctgggc
     3121 tgcgtttggt gagcgctcga tccgctcgcg gatgtactgg cccatatcga ctctcagata
     3181 aaggacggca gttacgatgg cggccaggac caacaggata atgccggcta cgagaaagcg
     3241 aggcctggcg cgcagggcct caaacgttct tgacggctcg aagaaga
//
LOCUS       DASUEX010000045         4411 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_814410, whole genome shotgun sequence.
ACCESSION   DASUEX010000045 DASUEX010000000
VERSION     DASUEX010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4411
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_814410"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            203..1540
                     /gene="eno"
                     /locus_tag="VFV61_00830"
     CDS             203..1540
                     /gene="eno"
                     /locus_tag="VFV61_00830"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016387099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEX4899040.1"
                     /translation="MSWIDLVQAREILDSRGNPTVEAEVTLVGGEVGRAAVPSGASTG
                     EHEAVELRDGDKKRYLGKGVLKAVRNVNDVIAPELEGLDALDQAEVDAALLALDGTKT
                     KSKLGANALLAVSLATARAAAEYLELPLYKYLGGPNARTLPVPMMNIINGGAHADNNV
                     DFQEFMVVPVGAATFSEALRMGAEIFHALKAVLKKRGYATSVGDEGGFAPNLRSNEEA
                     IETILESIPQAGYQAGSDCLLALDPAASEFYDGKSYVFKKSDKRKLSSDEMVAYWKSW
                     TTQYPIISIEDGMAENDWDGWKTLTDEIGKRVQLVGDDLFVTNTEFLRKGIELGVANS
                     ILIKVNQIGTLTETLDCIELAKTHGRTAVISHRSGETEDPFIADLAVATNAGQIKTGS
                     LSRTDRIAKYNQLLRIEADLRSAARYPGRSAFHQLAQNSENRVKDRRAANAKR"
     gene            1537..2016
                     /locus_tag="VFV61_00835"
     CDS             1537..2016
                     /locus_tag="VFV61_00835"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX4899041.1"
                     /translation="MTTMVESWQRGEFLISTDQRCLDREMIHDFLSRQSYWAKGRELE
                     VVNRGIDNSLSFGIYRGEQQIGFARVVTDYATFAWLADVFVLEEYRGRGLGKWLIEVI
                     LAHPQLQGFRRWALATKDAHELYRGFGFNELMRPERWMERPDPNMRESPDYWQKAHQ"
     gene            2042..3595
                     /gene="gpmI"
                     /locus_tag="VFV61_00840"
     CDS             2042..3595
                     /gene="gpmI"
                     /locus_tag="VFV61_00840"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HEX4899042.1"
                     /translation="MTTAKQSGPLALIIIDGWGYSPLREGNAIALAETPYYDQLLNDY
                     PHTLLEASGTRVGLPPGIMGNSEVGHLNIGSGRVIRMDVSRVDYDISTGDFFKNPVLL
                     TVTEELKRSGKSLHLLGLISDGQVHSSLEHLYALLRMARSQGLDQVFIHCFLDGRDTP
                     PSSAVQYIAQLQKQIDDIGCGRIATVVGRYYAMDRDKRWERTQRAYELLVNAVGRRSV
                     SAIDAIKHSYEQGTTDEFVEPIVIVSEAGEPVATIHDGDAVIFFNFRPDRARQLTRAL
                     AVAGFNEFPIAHRPQITFVCFTLYDRTFGLPIAFGPHDHHNVLAEIWGGKSVNNFRLA
                     ETEKYAHVTYFFNGGVEKEYPCERRLLVPSPRIATYDLQPEMSAFKVTRELLAAIEAN
                     ETNVFVVNFANPDMVGHTGKLDKTIEACQYVDTCLGWITKAIRLAGGVTMITADHGNA
                     EQMINLETGQPHTAHTSNPVPFHLIDDAARGLQLRAGGALEDVAPTILGLLGIEAPSE
                     MTGRDLREE"
     gene            3615..>4411
                     /locus_tag="VFV61_00845"
     CDS             3615..>4411
                     /locus_tag="VFV61_00845"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEX4899043.1"
                     /translation="MSFNPKKILVIDFGQLGDVVLSLPALRAIRAQFPRAQITIAIGK
                     PGAEVINLSGYGDDTIVVDRVALRDGFKPLSLFRVFELVKDVRRRKFDFVIDLHSLSE
                     TNLLGFLSGAPKRLYSRRHGRSLEYLANFHPRPPAESEDSQRHLIDRYLDVLIPLGVQ
                     DADRVPRLRTRKADDLVVDQILSKARVAPGTPLVGLFPGAGHPGRRWPLQKFSELADY
                     LMRNDDVRVLVFAGPEEQALVSEMRTMFPPRTLILDHLTISELAAALA"
BASE COUNT         1024 a         1124 c         1250 g         1013 t
ORIGIN      
        1 gcgccgtcga gtaatgcgcg tgaaattgac tctcacaccg gaaacctcgg aagcggaaag
       61 ccaacctacc gcagaagggg cacgagccgc cggttagaac aagatcctgt tcacgtctct
      121 ccttccttca atcacaccgc aacgtgataa agttgtcggt agaaaagcgc cttaacattc
      181 tttccgtgga gttgacttaa acatgagctg gattgatctg gtccaagcga gggagatact
      241 ggactcgcgc ggtaatccga cggtggaagc ggaagtcact ttggtgggcg gtgaagtggg
      301 gcgagccgcg gttccatcgg gtgcttccac cggcgagcac gaagccgtgg agcttcgcga
      361 tggcgataag aaacgctacc tgggaaaagg cgttctaaag gcggtgcgca acgtcaacga
      421 tgtaattgct ccggagcttg aaggtcttga tgccctcgac caggccgaag tcgatgcagc
      481 gctgctggca ttagacggga ccaaaacgaa atcaaagctg ggggcaaatg ctttgctggc
      541 cgtgtcgttg gcgaccgcgc gcgccgcagc ggagtattta gaattaccgc tctacaaata
      601 tctcggcgga ccaaacgcac ggacactgcc cgtgccaatg atgaacatca tcaacggagg
      661 tgcccatgcc gacaacaatg tcgacttcca ggagttcatg gttgtgccag taggcgctgc
      721 cactttttcc gaagctctgc ggatgggcgc cgagattttt cacgccttga aagcggtttt
      781 gaagaaaaga ggctatgcga ccagcgtcgg tgatgaaggt ggttttgctc ctaacctgcg
      841 ttcgaatgaa gaagcgattg agacgatcct ggaatcgatt ccgcaagctg gttatcaggc
      901 cgggagcgat tgcctgctgg cgcttgatcc tgcggccagc gagttctacg acggtaagtc
      961 gtatgtcttc aagaaaagtg acaaacgaaa gttatcgagc gacgagatgg tggcttactg
     1021 gaagagctgg acaactcagt atccgattat ttcgatcgag gacgggatgg cggagaacga
     1081 ttgggacggc tggaaaacgc ttactgatga aatcgggaag cgcgttcaac tagtcggcga
     1141 cgatctgttc gttaccaaca ccgaattctt gcgtaaggga attgagctcg gcgtagcgaa
     1201 ctcgattctg attaaagtca atcaaatcgg cactctcact gaaacgctcg actgcattga
     1261 actggcgaaa acccacggtc gaacggcggt gatttcacac cgttccggtg agaccgagga
     1321 tccattcatc gccgatctcg ccgtggctac gaatgccggc caaattaaga cgggttcgct
     1381 ttcccgaacc gatcggatcg cgaaatacaa tcagcttctg cgcatcgaag cggacttacg
     1441 atcggcggcg cgctacccgg gacgttcagc ttttcatcag ctcgcgcaga attcagagaa
     1501 tcgagtaaag gacaggcggg ccgcgaacgc caagcgatga ctacaatggt tgagtcgtgg
     1561 caaaggggcg agtttctgat cagcactgac cagcggtgcc tggatcggga gatgatccac
     1621 gactttttgt ctcggcaatc gtactgggca aaaggccgcg agttagaagt ggttaatcgc
     1681 ggcattgata attcgctcag ctttggcatc tatcgtgggg agcagcagat tggttttgcg
     1741 cgcgtcgtca cggactacgc caccttcgcc tggctcgctg atgtttttgt gctcgaggaa
     1801 tatcgcggcc gaggcctggg caagtggttg attgaagtga tcctggcgca tccgcaactc
     1861 cagggctttc gtcgttgggc gctggccaca aaagacgccc acgaactcta ccgaggtttt
     1921 ggtttcaacg aactaatgcg accggagcgt tggatggagc gacccgatcc aaacatgcgc
     1981 gaaagtcctg actactggca gaaagctcac caataatttc ttccctggcc aaccgattcc
     2041 gatgacaact gcaaaacaaa gcgggccgct cgcgctgatt atcatcgacg gctggggcta
     2101 ttcaccgcta cgtgaaggga atgcaattgc gcttgccgag accccttatt acgatcaatt
     2161 gctgaacgac tacccgcaca cactccttga ggcttccgga acgcgcgtgg gtttgccgcc
     2221 gggaatcatg ggcaattctg aagttggtca tctgaacatt ggctcgggcc gcgttatccg
     2281 catggatgtg tcgcgcgtgg attacgacat ctcgactgga gatttcttca aaaaccctgt
     2341 gctgctcacg gtgaccgagg agttgaaacg cagcggaaag tcactgcatt tgctgggatt
     2401 gatttctgac ggacaagtcc attcttcttt ggaacatctt tacgctctgc tgcgcatggc
     2461 gcggagccag ggactcgatc aagtcttcat ccattgcttt ctcgatggtc gcgatacacc
     2521 gccatcctcg gctgttcaat atattgcgca actgcagaaa caaattgacg acataggttg
     2581 tggaaggatc gctacggtgg tggggcgcta ttacgcaatg gatcgtgaca agcgctggga
     2641 acgaacccaa cgcgcttatg agctattggt aaacgccgta ggcagacgaa gcgttagcgc
     2701 tatcgatgcc atcaagcatt catacgaaca aggaacaacg gatgaatttg tagaaccgat
     2761 tgtgatcgtt tcggaagcgg gtgagccagt agcaaccatc cacgatggag atgccgtcat
     2821 ttttttcaac tttcgtcccg atcgtgcgcg ccagttaacc cgcgcgctcg cggttgcagg
     2881 gtttaatgag tttcccatcg cacaccgtcc ccaaattacg tttgtttgtt tcacgctcta
     2941 cgatcggacg tttggcctcc ccatcgcctt cggaccccac gaccatcaca acgtgcttgc
     3001 ggaaatttgg ggcggaaaaa gtgtcaacaa ttttcgactt gccgaaacgg aaaaatatgc
     3061 gcacgtaacc tacttcttta atggtggggt ggagaaggaa tatccctgcg aacggcgtct
     3121 cctcgttccg tcaccccgga tcgccactta cgatctgcaa ccggagatgt ctgcattcaa
     3181 agtgaccagg gagttattgg ccgcgattga ggcgaacgaa acgaatgttt ttgtcgttaa
     3241 ctttgcaaat ccggatatgg tcgggcatac cggcaagctc gacaaaacaa tcgaagcttg
     3301 tcagtacgtg gatacttgtt tgggctggat cacaaaagca attcgtcttg cgggtggcgt
     3361 cacgatgatt acggctgacc atggaaacgc ggagcaaatg atcaacctcg aaactggtca
     3421 accacacacc gcgcacacgt caaacccggt tccgtttcat ttgattgacg atgctgcacg
     3481 tgggttgcag ttgcgcgccg gtggggcgct tgaagatgta gcgccaacga ttctcggtct
     3541 gctgggaatt gaagcacctt cagaaatgac agggcgcgat ctgcgagagg agtaggcacc
     3601 ttgagtctcg gcaagtgagt tttaatccaa aaaagattct cgtgatcgac ttcggccagc
     3661 tgggcgatgt tgtcctcagt ttgccggctt tgcgcgcgat tcgcgcacag tttccccgcg
     3721 cacagatcac cattgcaatt gggaagccgg gtgccgaagt gatcaacttg tcgggttatg
     3781 gggacgacac gattgtcgtt gatcgagtgg ctttgcgcga tgggtttaag ccactgtcat
     3841 tgtttcgggt gtttgagtta gtgaaggatg tgcgccggcg caaatttgat tttgttatcg
     3901 acctgcacag tctttcggaa acaaacctcc tcggctttct ttccggtgca ccaaaacgac
     3961 tttattctcg tcgccatggg cgctctttgg agtatctcgc aaactttcac ccccgcccac
     4021 cggccgagtc ggaagactcc caacgacatt tgattgatcg gtacttggat gtactgattc
     4081 cgttgggcgt gcaggatgcc gaccgcgtgc cgcggctcag aaccagaaaa gctgacgatt
     4141 tagtagttga tcaaattctc agcaaagcaa gagtagcgcc agggacgcca ctagtaggat
     4201 tgtttcccgg agctggccat ccgggacggc gttggccctt gcagaagttt tctgagttgg
     4261 ctgattactt gatgcgcaat gacgacgtgc gcgtactggt gttcgcggga ccggaagagc
     4321 aagcgctagt cagcgagatg cgcacgatgt ttccgccccg cacattgatc ctggatcatc
     4381 ttactattag tgaacttgcc gccgctcttg c
//
LOCUS       DASUEX010000046         2441 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1043401, whole genome shotgun sequence.
ACCESSION   DASUEX010000046 DASUEX010000000
VERSION     DASUEX010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1043401"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="VFV61_00850"
     CDS             <1..439
                     /locus_tag="VFV61_00850"
                     /inference="COORDINATES: protein motif:HMM:NF018684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HEX4899044.1"
                     /translation="TFFSMQANNGSAPRQKRWLFIASCTLFLLITTSSGLAQRKKKTT
                     PCSSAQSQAEMNICWGKQYKAADIVLNQVYRELLLMLDEEQEAQLKKAQLAWLTYRDT
                     NCDFVADEYKGGSIRATILATCLLDMTSNRTRELKAQIKERNF"
     gene            447..1211
                     /locus_tag="VFV61_00855"
     CDS             447..1211
                     /locus_tag="VFV61_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002487914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HEX4899045.1"
                     /translation="MLQNKFGIVFGVANKRSIAWATAQSLHEAGARLAFTYQGDRLKE
                     NVESLTSEAMPGSLLLPCDVTKQEEVDATFKRVGEEFGRLDFLIHSIAYAPREALGGE
                     FLDTSREAFLTALEISAYSLGQLAKAAVPLMTEGGSIVCMSYYGAEKVIAGYNVMGVA
                     KAALEASTRYLARDLGPRNIRINAISAGPIQTLSARGVSDFSSMLKHHADHAALRRNV
                     EAREVGNTALFLCSPLSSAITGEVIYVDCGYSIMGI"
     gene            1266..2018
                     /locus_tag="VFV61_00860"
     CDS             1266..2018
                     /locus_tag="VFV61_00860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEX4899046.1"
                     /translation="MEKPTESDQRLPHATQDEQLLESPRTDEFTHTDTWRVFRIMGEF
                     VEGFDELASLTRGISIFGSARTPASDPYYKLAQETAALLAREGYAVITGGGPGVMEAA
                     NRGAFEAGGLSVGCNIELPFEQGANPYLTRSLTFRYFFVRKMMFVKYSLGFIIFPGGF
                     GTLDELFEALTLIQTHKIRNFPVVLFGTDYWQGLMDWIRNTALQEGKISEQDLNLLHL
                     TDSPEEVVNILNQSQSSLRVLDKTVSEEIREV"
     gene            complement(2034..>2441)
                     /gene="cutA"
                     /locus_tag="VFV61_00865"
     CDS             complement(2034..>2441)
                     /gene="cutA"
                     /locus_tag="VFV61_00865"
                     /inference="COORDINATES: protein motif:HMM:NF015077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HEX4899047.1"
                     /translation="RPRDMRPGHHYPTGIPTRITTPGTYSPLNNSVIIVLMTASSSEE
                     ADRLADMLVNGNLAACVQILPPIRSVYRWQGSIERSSEVLLIAKTVTDKFVELEQRVR
                     AVHSYETPEIVALPVTAISEPYREWLSASIDAE"
BASE COUNT          577 a          670 c          638 g          556 t
ORIGIN      
        1 tactttcttt tccatgcagg cgaacaatgg atccgctccg cggcaaaagc gatggttgtt
       61 tattgcttcc tgcacgctgt tcctgcttat caccactagc tcgggacttg cccaacgaaa
      121 gaagaaaacg actccctgca gcagtgccca aagtcaggcc gagatgaaca tttgttgggg
      181 caagcaatat aaggcggctg atattgtctt gaaccaagtc taccgcgagc tgctcctcat
      241 gctggatgag gagcaggaag ctcagctcaa gaaggctcaa ttagcgtggc taacatatcg
      301 ggatacgaac tgcgattttg ttgctgacga atacaagggc ggatccatcc gcgccaccat
      361 ccttgcaacg tgccttttgg atatgacgag caaccggaca agagaactta aggctcagat
      421 taaagagaga aacttctaaa caccatatgc tgcaaaataa attcgggatc gtctttggtg
      481 ttgccaacaa acgttccatc gcctgggcca ccgcacaatc cttacacgaa gccggcgctc
      541 gtctcgcctt tacttatcag ggcgatcgcc tgaaggaaaa tgtcgaaagc ctgactagcg
      601 aagcgatgcc cggctcgttg ctcttacctt gcgacgtgac caagcaggaa gaagtggacg
      661 ctaccttcaa acgcgtcggt gaggagtttg gcaggctcga ctttctgatt cacagcattg
      721 cctatgcgcc acgcgaagct ttgggcggag agttccttga taccagccgc gaggctttcc
      781 ttacggcact tgaaatcagt gcatactcgc tcggccaact cgcaaaagcc gctgtgccct
      841 taatgacgga aggtggcagc attgtctgta tgagttatta cggcgccgag aaagtaatcg
      901 cgggttataa cgtgatgggc gtcgccaaag ccgcgcttga agccagtacc cgttaccttg
      961 ccagggacct cggaccgcgc aacatccgca tcaacgccat cagtgccgga ccaatccaaa
     1021 cactctctgc ccgcggcgtc tcagacttca gctcgatgct gaaacatcac gccgaccacg
     1081 cggcgctgcg acgcaatgtt gaagcccgag aagtcggcaa cacggcgctt ttcttgtgta
     1141 gcccgctctc gtccgcaatc acgggtgaag taatttatgt cgattgcggt tacagcatca
     1201 tgggaatcta accctggaga ggttcgcgcg tcacgacgca aactagtagt ctgcgctaga
     1261 aaaacatgga gaaaccaacc gaaagcgatc agcgactgcc gcacgcgact caggatgaac
     1321 aactccttga gagtccgcgt acggacgagt ttactcacac tgacacctgg cgcgtgtttc
     1381 gcatcatggg cgagtttgtc gaaggcttcg atgaacttgc cagcctgaca cgcggcatct
     1441 cgatctttgg ctcggcgcgg actcccgcaa gcgatcccta ctacaaactg gcgcaggaaa
     1501 ctgcggcgct cctcgctcgt gaaggctatg cagtgattac cgggggcgga ccgggggtta
     1561 tggaggcggc gaaccgcggc gccttcgagg ccggagggct ttcagtcggg tgcaacatcg
     1621 aactgccttt tgagcagggc gcgaatcctt acctgacccg gtcactgact ttccgttact
     1681 tcttcgtcag aaaaatgatg ttcgtcaaat atagtctcgg cttcattatt tttcccggcg
     1741 gctttggcac tctcgacgaa ctgttcgaag cgctaacctt gatccagacg cacaagattc
     1801 gaaacttccc cgtagtacta tttgggactg actactggca aggtttgatg gactggatac
     1861 gcaatactgc tctgcaggaa ggaaagattt ccgagcagga tcttaattta cttcatctca
     1921 ccgactctcc tgaagaggtc gtaaacatcc tcaaccaaag ccagagttcc cttcgcgttc
     1981 tcgacaaaac cgtctccgaa gaaattcgcg aagtctaggc ccgcggcacg cgctcactca
     2041 gcatcaatgc tggcggagag ccattcacga tatggctcgg aaatcgccgt taccggcaac
     2101 gcaacgatct ccggtgtttc gtaactgtgc actgccctga ctcgctgctc cagctcaaca
     2161 aacttgtctg ttacggtctt ggcaattaac agcacttccg atgatctttc gatacttccc
     2221 tgccaacgat aaaccgatcg aattggtggc aggatttgta cgcaggctgc gaggttaccg
     2281 ttaaccagca tatcggcaag cctgtcagct tcttcacttg aggaggccgt cattaatacg
     2341 atgatgactg aattgttcaa gggactgtag gtgccaggtg tcgttatacg tgtcgggata
     2401 cctgtcgggt aatggtgtcc gggtctcatg tcccgtggtc g
//
LOCUS       DASUEX010000047         2035 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1380788, whole genome shotgun sequence.
ACCESSION   DASUEX010000047 DASUEX010000000
VERSION     DASUEX010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1380788"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(258..>2035)
                     /gene="acs"
                     /locus_tag="VFV61_00870"
     CDS             complement(258..>2035)
                     /gene="acs"
                     /locus_tag="VFV61_00870"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEX4899048.1"
                     /translation="FKKWDQVLHWEAPHAQWFVGGRLNISYNCLDRHLSTWRRNKAAL
                     IWEGEPGDQRTLTYQQLHGEVSRFANVLRHAGVATGDRVALYMPLVPELAIAMLACAR
                     IGATHSVIFGGFSSAALIDRINDASCKVVVTADGGWRRGGEVKLKAAVDEALKQTPSV
                     KSCIVVRRTGVSVEMKAGRDFWWHELMEAVDGNCDAVELDSEHPLFILYTSGTTGKPK
                     GILHTTAGYLLQAHLTTKWIFDLKDEDIYWCTADIGWVTGHSYVVYGPLSNGATVLMY
                     EGAPNYPEPDRFWSIVERHRVNILYTAPTAIRAFIKWGEHWPLRHDLSSLRLLGTVGE
                     PINPEAWMWYREVIGKGACPIVDTWWQTETGAIMISPIPGATPTKPGSATKPFPGIEA
                     DVMTRDGKSVAPNEGGYLVIKRPWPAMLRTIWGDDDRYREQYWSQLDGIYFAGDGARR
                     DEDGYFWIMGRIDDVINVSGHRLGTAEVESALVSHEAVAEAAVVARPDDLKGSAIVAF
                     VTLEGSHHASEQLKQELRVHVTKEIGALARPDEIRFSDMLPKTRSGKIMRRLLRELAT
                     SGAVAGDVTTLEDFSVLEKLRTEEE"
BASE COUNT          445 a          607 c          541 g          442 t
ORIGIN      
        1 tgtgcgcgcc gccgggctcg tcccactcgg ctcgcacttc gaaagacgcg aggtagcgcg
       61 gacccgagcg attgttgatt tcgatatcgc gagtcaatgg caactcctta tctcaatgag
      121 caactacgcg tcagcgcagc gcccaccaga actcagtcac aagcgtcgca agtctataac
      181 ctattaagtc tcaagtctca agtctcaggt ctcaagtctc gaaacctgaa acttgaaacc
      241 tgaaacccgg aacctgccta ctcctcttct gtccgaagtt tctccaacac cgaaaaatct
      301 tcgagtgtag ttacatctcc agccacggcc ccactggtcg caagttcgcg cagcaggcgg
      361 cgcatgattt tcccggagcg cgtcttgggc aacatatcgc taaaacggat ctcgtcgggt
      421 cgagcgagag caccaatttc cttggtaaca tggacccgca attcctgctt cagttgttcc
      481 gaagcgtgat gtgaaccttc aagggttaca aaggcaacga tcgccgatcc ttttagatcg
      541 tccggacgcg ccactaccgc ggcttcggca acggcctcat gtgatacgag tgcgctttcc
      601 acttccgccg ttcccagacg atgaccgctc acgttaatca cgtcatcgat acggcccatg
      661 atccagaagt aaccatcttc gtcgcgccgg gccccgtcgc cagcgaaata gatgccatca
      721 agctgcgacc agtactgctc gcggtaacga tcgtcgtcgc cccagatagt gcggagcatt
      781 gccggccatg gtcgtttgat aacaaggtaa ccgccttcgt tgggcgcgac ggacttccca
      841 tcacgagtca tcacatcagc ttcaattccg ggaaagggtt ttgtcgcgga accaggtttt
      901 gtaggggtgg cgccaggaat cggcgagatc atgatggcgc cggtttcagt ctgccaccag
      961 gtgtctacga ttgggcaggc gccttttcca atcacttcgc gataccacat ccacgcttcg
     1021 gggttgatcg gctcccctac cgtgcccagc aaacgcaaac tggataagtc gtgacgcagc
     1081 ggccagtgtt ctccccactt aataaaagcg cggatagcgg ttggtgcggt gtagagaatg
     1141 ttgacccggt ggcgttcaac gatactccaa aagcgatccg gttccggata atttggggct
     1201 ccttcgtaca tcaacacggt ggcgccgttt gagagcggac catagacaac gtaactgtgt
     1261 ccggtcaccc aaccgatgtc tgccgtgcac cagtagatgt cttcgtcctt gagatcgaaa
     1321 atccatttgg tggtgagatg tgcctgcaga agataaccgg cggtagtgtg cagaatgccc
     1381 ttgggctttc ccgtagtccc ggaagtgtag aggatgaaca atgggtgctc gctgtccaac
     1441 tccacagcgt cgcaatttcc gtcaacagcc tccatgagtt catgccacca gaaatcgcgg
     1501 ccggctttca tctcgacgct gacaccggta cggcgcacca cgatgcagga tttgaccgag
     1561 ggagtttgtt tgagagcctc gtccaccgca gctttcagtt tcacttcgcc gccgcggcgc
     1621 cagccaccgt ctgcggtcac gaccactttg cagctcgcgt cgttgatgcg atcaatcaac
     1681 gccgcactcg agaaaccgcc aaagatcacc gaatgagtgg ccccgatgcg cgcgcaggca
     1741 agcatcgcga tcgccaattc gggaaccagc ggcatgtaga gcgccacacg atctccggtt
     1801 gcgactccag catgtcgcag tacattcgcg aaacggctca cttcgccgtg aagctgctgg
     1861 taggtcagcg tgcgttgatc acccggttcg ccttcccaaa taagagcggc cttgtttcgt
     1921 cgccaggtgg aaagatgtcg atccagacag ttgtaggaaa tatttagcct gccgccgacg
     1981 aaccattgcg catgaggggc ttcccagtgg agtacctggt cccatttctt gaacc
//
LOCUS       DASUEX010000048         2327 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_585526, whole genome shotgun sequence.
ACCESSION   DASUEX010000048 DASUEX010000000
VERSION     DASUEX010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_585526"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1393
                     /locus_tag="VFV61_00875"
     CDS             <1..1393
                     /locus_tag="VFV61_00875"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEX4899049.1"
                     /translation="AGVVTAVGRVEGREVVVVANEATVKAGSWWPETIKKILRAQEIA
                     MRSHVPIIYLVDSAGVNLPYQGGVFPGQYGASRIFYYNSIMRRYLKIPQISAVMGPCI
                     AGGAYLPALSDTIIMVEGTSFMGLGGANLVKGATGQTIDNETLGGARTHNEISGVAHY
                     RVADDAACLAKIREFVSELPSKSSSAVSLAEVREAARPAADLYDIIPEDHRQPYNIRH
                     LLDCILDEGHLDEFQADYAKEMITGHGRIRGIQVGVIANNRGMIRVPGGPPRFGGIVY
                     TESAEKVAYFIETCNRHRTPLVFIQDVSGFMVGPEAEHSGIIRAGARFVEAMATATVP
                     KLVLTINHASGAGYYAMAGQGFDPNFIFSWPTGRMGVMEGDSAVQAVFGSQLEKLKRD
                     GAGPDEQLNAEMDKVRETYERELDAKYAAARGFVDAVVSPEATRDALELALRTSLNYG
                     GPHLGQFVLPPSL"
     gene            1453..>2327
                     /locus_tag="VFV61_00880"
     CDS             1453..>2327
                     /locus_tag="VFV61_00880"
                     /inference="COORDINATES: protein motif:HMM:NF018938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HEX4899050.1"
                     /translation="MKEKIRIAAGQGFWGDLLDAPVRQVEEGPIDYLMLDYLAEVTMS
                     IMQKQRSRDPAAGYARDFVPLMKQILPALVEKNIRVTANAGGVNVVGCANAVREVARE
                     LGLAGKVKIGVVTGDDIMARLDEMLARGVELRNMDTDEPLSTVRNRIQSANAYLGAWP
                     MVEALDQGAQIVITGRATDTGLSLAPMIHEFCWARDDWNRLAAGTIAGHIIECGAQSS
                     GGNCQYEWRSIKNLANVGFPIAEASPDGTFVITKHDNTGGCVNVPSVKEQLVYEMGDP
                     HEYITPDVVADFTSIR"
BASE COUNT          494 a          610 c          714 g          509 t
ORIGIN      
        1 ggcaggtgtc gtgacagcgg tcggacgagt cgaaggccgc gaggttgtcg tagtagcgaa
       61 tgaagcgacg gtgaaagccg gttcctggtg gcctgagacc atcaagaaga tcttgcgagc
      121 gcaggagatt gcaatgcgct cacacgtacc catcatctat ctcgttgact ccgcgggcgt
      181 aaaccttcca tatcagggtg gagtttttcc cggacagtac ggcgcctcac gcatcttcta
      241 ttacaactcg atcatgcggc gatatctgaa gattccccag atctcggcgg tgatgggacc
      301 atgcatcgcc ggcggtgctt acctgccggc actttccgac accatcatca tggttgaagg
      361 cacttctttc atggggctag gcggggcgaa tttggtgaag ggagcaaccg ggcagacgat
      421 tgacaacgaa acgcttggcg gcgcccgcac gcataacgag atctctggtg ttgcgcatta
      481 ccgcgtggcg gacgatgcgg catgtctcgc caagatccgg gagtttgttt cagaattgcc
      541 gtcaaaatcc tcatctgcgg tttcgcttgc agaggtgagg gaagcggccc ggccagccgc
      601 agatctctac gacatcattc cagaagatca ccggcagccg tacaacattc gtcatttgct
      661 ggactgcatt cttgatgaag gtcacctcga tgaatttcag gccgattacg caaaggagat
      721 gatcacgggt cacggccgaa ttcgtggcat tcaggtggga gtgattgcga ataaccgcgg
      781 catgattcgc gtccccggtg gaccaccccg ttttggcggc atcgtctaca ctgagtctgc
      841 tgagaaagtc gcctacttca tcgaaacctg caataggcat cggacgccac tcgtctttat
      901 ccaggacgtg tcgggcttta tggttggtcc cgaggcggaa cactctggca tcattcgggc
      961 tggtgcgcgt ttcgtcgaag cgatggcgac agctacggtg ccaaagctcg tcctgacgat
     1021 caatcatgcg tcgggtgccg gttattacgc catggccggt cagggattcg atccaaattt
     1081 catctttagc tggcctactg gccgcatggg cgtgatggaa ggtgactcag cagtgcaggc
     1141 ggtgtttggc agccaactcg aaaaactcaa acgcgacggg gccggaccag acgagcaact
     1201 caatgccgag atggacaagg tgcgcgagac ttatgagcgg gagctcgacg cgaaatatgc
     1261 ggcagcgaga ggatttgtcg atgcggtggt ttcgccggaa gcaactcgcg acgcgcttga
     1321 gctcgctttg cgtacgagct tgaattatgg cggcccacat ctgggccagt ttgtgctgcc
     1381 gccgagtctt taaggtgcgg agttaacgtt tagttttgtg atcggctcaa catttcggca
     1441 agccaattcc atatgaaaga aaagattcgt attgcagcgg gccaggggtt ctggggtgac
     1501 ctcctcgatg cgcctgtgcg ccaggtggaa gaaggtccga tcgactatct catgctcgat
     1561 tatctcgccg aagttaccat gtcgatcatg cagaagcagc gctcgcgcga ccctgcggct
     1621 ggctatgcca gggattttgt tccactgatg aagcagatcc ttcccgcgct ggtggaaaag
     1681 aacattcgcg tgactgctaa cgcgggcggg gtgaacgttg tgggctgcgc gaacgcagtg
     1741 cgtgaagttg cccgggagtt gggtctcgcc ggaaaagtga agattggcgt cgtcaccggc
     1801 gatgacatca tggcgcggct ggatgagatg cttgcgcgcg gggtcgaatt gcgcaacatg
     1861 gatacggatg agccactatc caccgtccgc aacagaattc agagcgcgaa cgcttatctc
     1921 ggtgcgtggc cgatggttga ggcgctggac cagggtgccc agattgttat cactggccgg
     1981 gccaccgaca ctggtttgag tctcgctccg atgattcacg aattttgctg ggcccgagac
     2041 gattggaacc gtctcgcagc cggaaccatc gctgggcaca taatcgaatg tggcgcccag
     2101 tcctcgggtg gcaactgcca gtacgaatgg cgcagcatca agaatctggc caacgttggt
     2161 ttcccgattg cggaagcgtc cccggacggc acctttgtga tcacaaaaca cgacaacacc
     2221 ggtggctgcg ttaatgttcc ttcggttaag gagcagttgg tctatgagat gggcgacccg
     2281 catgaataca tcacaccgga cgtggttgcc gatttcacga gcattcg
//
LOCUS       DASUEX010000049         2205 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_585562, whole genome shotgun sequence.
ACCESSION   DASUEX010000049 DASUEX010000000
VERSION     DASUEX010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_585562"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1260
                     /locus_tag="VFV61_00885"
     CDS             <1..1260
                     /locus_tag="VFV61_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899051.1"
                     /translation="FSVPFFGTNHNNLTVSSNGNLYFSPPPTRDNGDADDVPSATTSL
                     AHFKMISGLWDDIDLSTSARADSGVYVVQPDATRIIFRWQGVPCNFNGTACQGGGPVN
                     FEIELRIDGTIKSRYGSGNTNLFPVVGISGGSPDAYVITSHTSEDVSMSLTNAVEVTY
                     IPRNIINPLDNSYFFTSQQYRDLLGRENDLGGLAFWANQINECNPADIICLINRRVNV
                     SGAFFVENEFQRTGSFVYRSYKGGLGRRPTYAEFTGDRPLIVEGPNLEQTKQAYSLAF
                     VQRAEFVAKYNGLNTADAFVDELIATIQASSNVNLAPQKTSLINAYNGSGDPTTSRAL
                     ALRAAIDNTAFTTAEYNPSFVLMQYFGYLTRDIDQGGYDFWLGVLNGPQLNNYRGMIC
                     AFITSQEYQKRFSSISPHTDVECGYLN"
     gene            1393..>2205
                     /locus_tag="VFV61_00890"
     CDS             1393..>2205
                     /locus_tag="VFV61_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899052.1"
                     /translation="MQTLRPTIILVLLLTVGTSVDALGKGVPQRAKLPEAKQKKRTVV
                     RTRKQSHKPRPSVFESVSDEGDNVEGRQSWFMFQRLYPFRTLPTEARRRAWESRPLRA
                     KSAPGVENATASVWSPIGPMPTRAYFPLFDNFGFNSGRINAIAVSPANAQLILVGAST
                     GGIWRSIDGGATFTPVSDAQVDLAVGAIAFSPSNPNIVYAGMGDMHSCCEYLGSGVLK
                     STDSGATWVRINNGTLPQPASVGDLEVDPTNSNKIYLALYRSLDAANANSFPY"
BASE COUNT          486 a          611 c          586 g          522 t
ORIGIN      
        1 ttcagcgttc cattcttcgg cactaaccat aacaacttaa cggtttcgtc gaacggaaat
       61 ctctacttct cacctccgcc aaccagagat aacggcgacg cggatgatgt tccgagcgca
      121 accacttcgt tggctcattt caagatgatc tcgggattgt gggacgacat tgacctcagt
      181 acgtccgctc gtgccgattc cggtgtttat gtcgttcagc cagacgcaac ccggataatc
      241 ttccgttggc agggtgtgcc ctgtaacttt aacgggacgg cttgccaggg tggcgggccc
      301 gtcaactttg agatcgagct tcgcatcgac ggcaccatca agagccgcta tggcagtggt
      361 aacactaatc tattccccgt ggttggcatt tcaggcggtt caccagatgc ctatgtaatc
      421 acgagccaca cgtcggaaga tgtgtccatg agtctgacga acgcagtgga agtgacctac
      481 attccccgca acattatcaa cccgctcgat aacagttact tcttcaccag ccagcagtac
      541 cgagatctgc tggggcgcga aaatgatcta ggtggtctag cgttctgggc caaccagatc
      601 aatgaatgca atccggccga tatcatctgc ctgatcaatc gtcgcgtgaa tgtttcgggt
      661 gcgttctttg tcgaaaacga gttccagcga accggatcat tcgtgtaccg ctcatacaag
      721 ggcgggcttg gccggcgtcc aacgtacgca gagttcacgg gcgaccggcc gctgatcgtt
      781 gaaggaccaa atctggagca gacgaaacag gcttactcac ttgctttcgt tcagcgcgcc
      841 gagtttgtgg cgaaatacaa cgggcttaat actgctgacg ctttcgtcga tgaactgatt
      901 gcgactattc aggccagctc gaacgtgaac ctggcaccgc agaaaacgtc gctcataaat
      961 gcttacaacg gtagtggaga tccaactacg agtcgtgccc tggcgcttcg ggccgcgatc
     1021 gataacacgg cgttcaccac tgcggaatac aacccgtcgt tcgtgttgat gcagtacttc
     1081 gggtacctga cgcgcgacat tgaccagggt ggatacgact tctggctcgg cgtcctgaac
     1141 ggtccacaac tgaataacta ccgcggcatg atctgcgctt tcatcacctc gcaggagtat
     1201 cagaaacgat ttagttcgat ttcgccgcat acagatgttg agtgtggcta tttgaactag
     1261 cccggatctc aagccgagga ccgggggact acccggttct cgcttgggct ttcacctcgc
     1321 ttagcacttg ccagcggttc tccctccctc tggtgagata tcctttgctc gttagtttgt
     1381 gcaaagtcct caatgcaaac cctacgaccc acaatcattc tggttttgct tttgaccgtc
     1441 ggcacttctg ttgacgcctt ggggaaaggt gttccgcaac gagcaaagtt gcctgaggca
     1501 aaacagaaaa agcgaacagt cgttcgcacc aggaaacaga gccacaagcc acgtccctct
     1561 gtattcgagt cagtgagtga tgagggcgac aatgtcgagg gacggcaaag ctggttcatg
     1621 tttcagcggt tgtatccgtt tcgcacgctt cctacagaag cgcgacgccg agcttgggag
     1681 tcccggcctt tgcgtgccaa atcggcgccc ggggttgaaa atgccaccgc ctcagtttgg
     1741 tcgccaatcg gtcccatgcc gacgcgtgcc tactttccgt tgttcgataa cttcggtttc
     1801 aatagtgggc gcatcaacgc gatcgcggtt tctcctgcaa acgctcaact gattttggtg
     1861 ggagcctcaa ccggtggaat ttggcgttcc atcgatggtg gcgcgacttt tacgccggtg
     1921 agcgacgctc aagtggatct ggcggtaggc gcgattgcgt tttcacccag caatccaaac
     1981 attgtctatg ccggcatggg cgatatgcac agttgttgcg aatacctggg cagcggcgtt
     2041 ttgaagtcga cggacagtgg cgccacctgg gtgcgaatca acaacggaac cttgccgcaa
     2101 cctgccagcg tcggtgacct cgaagtggat ccgacaaatt caaacaagat ctacctggca
     2161 ctttatcgct cgctcgatgc cgcaaacgcg aattcttttc cttac
//
LOCUS       DASUEX010000050         3456 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_471035, whole genome shotgun sequence.
ACCESSION   DASUEX010000050 DASUEX010000000
VERSION     DASUEX010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471035"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1317
                     /gene="mpl"
                     /locus_tag="VFV61_00895"
     CDS             <1..1317
                     /gene="mpl"
                     /locus_tag="VFV61_00895"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: protein motif:HMM:TIGR01081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HEX4899053.1"
                     /translation="VYPPMSTMLSSLGIEVKHGYSASHLTPAPDCVVVGNAIPRGNPE
                     VEETLKRKLLYRSQAEVVKEEFIRGRRSLVVAGTHGKTTTTGIAAWVLEQGGLNPTFL
                     IGGIAQNFGASFRVTESDYFVIEGDEYDTAYFDKGPKFMHYLPELAIVNNIEFDHADI
                     YPDLAAVKLAFRRFMNLVPADGRLIVGWDSSNVREVVASFGKRLFTQLETFGMSPDAK
                     WQVSDADYSGELTSFKVLREGQEWGQFTTTLIGDFNLLNSLAVIIAADAWGMERETIA
                     AALASFKNVRRRAEVRGEVAGITVIDDFAHHPTAVRETLNALRQKYQQRRLVAIFEPR
                     SWSSRLAVFQHEYAEAFAAADYVVIANVFDSQKVTEKGRMLDTVELIKDISRGNKPAL
                     ALPGADDIVAHLLPQLREGDVVAIMSNGGFGGIHEKLLTQIKTRLA"
     gene            complement(1314..1775)
                     /locus_tag="VFV61_00900"
     CDS             complement(1314..1775)
                     /locus_tag="VFV61_00900"
                     /inference="COORDINATES: protein motif:HMM:NF018508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HEX4899054.1"
                     /translation="MAALKARARLKSLLLFIPNMVLLCGRLLTDARVPKTEKALVAGA
                     ILYAIIPLDLIPDVIPFVGQIDDAYLIAITLLRLIDRTDPKVVREHWNGGGDIVALIE
                     MLAMMTAKFLPARIRRVLTSRVEVAPKSKDKGLPIPALVVKPEIASSGKRD"
     gene            1920..2933
                     /gene="pta"
                     /locus_tag="VFV61_00905"
     CDS             1920..2933
                     /gene="pta"
                     /locus_tag="VFV61_00905"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEX4899055.1"
                     /translation="MNILETLRQRAAGNPQHIVLPEGNDPRTVVAAAICTRQNIARIT
                     LLGDEAIIRETAERERADLGGVTVIDHKRAADFEKLANTFHELRRAKGLMADEARTML
                     ADPLYYGNLMVRLGAADGSVAGATNTTSHTVRAALLCIGVRSGFKLVSSFFLMSLRDS
                     GFGHDGAMIYADCGVVIEPTAAELAEIALASADSCRALLGTTPRVGMLSFSTKGSAKH
                     RLVDKVVEATRTAKARAPELIIDGELQADAALMPEVSESKSPGSAVGGRANVLIFPDL
                     QSGNIAYKLTERLAGATAIGPILQGLDKPANDLSRGCKAEDIVDAVAITAVQAQARKS
                     SQA"
     gene            3004..>3456
                     /locus_tag="VFV61_00910"
     CDS             3004..>3456
                     /locus_tag="VFV61_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899056.1"
                     /translation="MPTPRYSFLTLLLVVVATSIPAWGQAGRRPEPSPTPPHQEQEPV
                     RVFTEEVRLPVIALDQFGHYDPTLELDDVLVLEDGVAQQVRSVRHIPANVLLLLDTGG
                     ELSGLGGSSKLTSLTRDVAIQVLQSVPDGTSLAVMQFNNSVELLQPWTA"
BASE COUNT          745 a          941 c          971 g          799 t
ORIGIN      
        1 gtttatccac ccatgtcgac aatgctctcc tcgctcggga ttgaagtgaa acatggctac
       61 agtgcctcgc atctgactcc tgctcctgat tgtgtggtgg tcggcaacgc gatcccgcgc
      121 ggcaatcccg aagttgaaga gacattgaaa agaaaactgc tttatcgctc gcaagcggaa
      181 gtggtgaagg aagaattcat acgcggacgg cgatcacttg tggtcgccgg cacccatggc
      241 aaaacgacga ccaccggtat cgctgcgtgg gtgttggagc aaggcgggct taacccaacc
      301 ttcctgatcg gcggcattgc ccagaacttt ggcgctagtt ttcgcgtgac tgaaagtgac
      361 tactttgtca ttgaaggtga tgagtatgac accgcttatt tcgataaagg cccgaagttt
      421 atgcattatc tgccggagct ggcgatcgtc aacaacatcg aattcgatca cgccgacatc
      481 tacccggatc tcgccgctgt aaaactcgct ttccgtcgct tcatgaattt agtaccagct
      541 gatggtcgtt tgattgtggg ttgggacagc agcaacgtgc gcgaagttgt cgcgtctttc
      601 ggcaagaggc tctttacgca actggaaacg tttggcatga gtcctgatgc gaaatggcaa
      661 gtgagtgatg ctgattattc aggcgaactg accagtttca aagtgctgcg cgagggtcag
      721 gaatggggtc agttcacaac gacgctgatc ggcgacttca acctgctcaa ttctttggct
      781 gtcatcatcg cggccgatgc ctggggaatg gaaagagaga cgatcgccgc agccctcgca
      841 agtttcaaga acgtgcgccg gcgggcggaa gtgcgcggag aagtcgccgg cattacagtg
      901 attgacgact tcgctcatca tccaaccgca gtacgtgaaa ccttaaacgc gctgcgtcag
      961 aagtatcagc agcgccggct ggttgcaatt tttgaacccc gatcgtggtc gtcgcgcctg
     1021 gctgtgtttc aacatgagta tgccgaggct ttcgcagcgg ctgactatgt tgttattgcc
     1081 aacgtttttg atagtcagaa ggtgactgaa aaaggccgca tgttggacac ggtggaacta
     1141 atcaaagata tttctcgagg caacaaaccg gcgctcgctt tgccgggcgc ggacgacatt
     1201 gttgcgcatc tgctgccgca attacgtgag ggagacgtgg tcgcgatcat gtctaacggc
     1261 ggcttcggcg ggattcacga aaaacttttg actcaaataa agacgcgcct ggcttagtct
     1321 cttttcccag acgaggctat ttcgggcttg accaccaagg cggggattgg caagccctta
     1381 tctttggatt ttggtgccac ttcgaccctc gaagttagca cgcgtcggat cctggcgggc
     1441 aagaacttcg cggtcatcat ggccaacatt tcgatcaaag ccacaatatc acccccaccg
     1501 ttccagtgtt cccgcaccac tttgggatcc gtacgatcaa ttagtctcaa cagcgtgata
     1561 gcgatcaaat aggcgtcgtc gatctgtccc acaaatggaa tcacatcggg aatcaaatcc
     1621 agcggaatga tggcatagag gatcgctccg gcaaccagcg ctttctccgt tttcggcacg
     1681 cgcgcgtcgg tcaacaaccg gccgcaaagc agcaccatgt taggaatgaa taacagtaaa
     1741 cttttgagtc gggctcgcgc ctttaatgct gccatgtctt tctccgaagc agtttgctca
     1801 gctaacactt aggtggattt agtaactaaa gttaacacga ctgccggctg actcaaacac
     1861 ttgtcgtctc cttccgagtt ggttagactc gctcccggca atccggacca ctcaacccta
     1921 tgaacatact tgagacactg cggcagcgcg ctgcgggcaa tccgcaacac attgttttgc
     1981 cggaaggcaa cgacccgcgc actgtcgtag ccgcggctat ctgcacgcgc cagaatattg
     2041 cgcgcattac cttgctgggc gatgaagcga ttattcgtga gactgccgag cgggagcgcg
     2101 ccgacctggg cggcgtaacg gtgattgatc acaagcgtgc tgctgatttt gaaaagctgg
     2161 ccaacacctt ccacgagttg cggcgggcga aaggcttgat ggccgacgag gcgcgcacta
     2221 tgttggccga tccgctttac tacgggaatc tcatggtccg actcggtgcc gctgacggtt
     2281 cggttgccgg agctacgaat acaacgtcgc acacagtgcg ggcggcgctg ctctgcatcg
     2341 gagttcgctc gggattcaaa ctggtttcca gtttctttct gatgtcgttg cgcgacagcg
     2401 gctttggaca cgacggcgcg atgatctatg ccgattgcgg cgttgtgatc gaaccaactg
     2461 ctgcggaact tgccgagatt gccctcgctt ctgctgattc gtgccgtgct ttgctcggta
     2521 ccacgccacg agtcggcatg ctttcgttct cgacgaaggg gagcgccaag catcgcctgg
     2581 tagataaagt cgtggaggcg acgcgcacgg ctaaagcgcg cgcgccggaa ttgattatcg
     2641 atggcgagtt gcaggccgac gcggccttga tgccggaggt gtctgaatcc aagtcacccg
     2701 gttcggccgt tggcggtcgc gccaatgttc tgatctttcc cgacctgcaa tcgggaaata
     2761 tcgcgtacaa acttacggaa cgacttgctg gagcgactgc tattggtcct atcctgcaag
     2821 gactcgacaa gccggccaac gacctttcgc gtggctgtaa agcggaagac atcgttgacg
     2881 ccgtcgcgat caccgcggtt caagcccagg ctcgtaagtc atctcaggcg tgacgggcaa
     2941 cgcccagccg gtccgccagg catccaaggg cccgactaaa tcaattggcc aggtcaggtg
     3001 ctcttgccaa ctcccagata ttcgtttttg actctcttgc tggtcgtcgt cgctacctct
     3061 atccctgcct ggggtcaggc ggggcggcgg cccgaaccgt cacccactcc acctcatcaa
     3121 gaacaagaac cagtgcgagt atttacggag gaagttcgtt tgcccgtaat cgccctcgat
     3181 cagtttggac actatgatcc cacgctcgag ctcgatgacg ttttggtgct cgaagatggt
     3241 gtggcccaac aagtgcggag cgttcgtcat atccccgcca atgttttgtt gttacttgat
     3301 accggcggtg agttgagcgg actcggtggg tcttcaaagc ttacatcgct aacacgtgac
     3361 gttgcgattc aagtactgca aagtgtgcct gacggtacct cgctcgcggt gatgcagttc
     3421 aacaactcgg ttgagttgct gcaaccctgg actgcc
//
LOCUS       DASUEX010000051         3976 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1381007, whole genome shotgun sequence.
ACCESSION   DASUEX010000051 DASUEX010000000
VERSION     DASUEX010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3976
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1381007"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1013)
                     /locus_tag="VFV61_00915"
     CDS             complement(<1..1013)
                     /locus_tag="VFV61_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899057.1"
                     /translation="MQRRQKIVFVVVLLILLSQLPFAYRRRRLVRLQATIQQLNAERN
                     TSSVDPGFSEYREYKGVVHVHSFLGGHSSGTFQEIIAAAKTNQLDFVVMTEHTERDIN
                     TAAMTLHGVHGGILFLNGNEVSAKDAGRVLLIPGLETQAAEDASLEDLAQQVRAKLGL
                     SIVAYPEEFEGWNTNAYDGIEVYNLYTNAQQINPLMMFFDGLWCYRSYPELLFANFYR
                     RPNESLRLWDQTLARGQRLTALAGVDAHANIGIGLQDASGKTLIGLLLDPYERSFGIV
                     RVHALLSERDGFNPESVLGALRSGNSFIGFDLLADSTGFRFSGRSGGEAGMQGEELKL
                     QGEA"
     gene            complement(1086..1376)
                     /locus_tag="VFV61_00920"
     CDS             complement(1086..1376)
                     /locus_tag="VFV61_00920"
                     /inference="COORDINATES: protein motif:HMM:NF019491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HEX4899058.1"
                     /translation="MLTHIVIWKYRDDVEAEVRETHLSMLRSLAAAIPVIESLAVGFD
                     VLKLPRSYHTGLVAVFRDRAALQTYTVHPEHVKVVAFGGAITEQIASVDFET"
     gene            complement(1393..1854)
                     /locus_tag="VFV61_00925"
     CDS             complement(1393..1854)
                     /locus_tag="VFV61_00925"
                     /inference="COORDINATES: protein motif:HMM:NF015331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX4899059.1"
                     /translation="MLFVKESLIHANPNRVFAFHERDNALELLMPPWETARVLQRARI
                     ADVGSRAIIETNFIGPLKTRWVAEHTRYEPPNFFEDVQIKGPFRSWKHRHIIAADPEG
                     ATLRDEIEYEPPLGALGRLFAPILIERRLRKLFDYRHQVTRASCEAESAKY"
     gene            complement(1933..>3976)
                     /locus_tag="VFV61_00930"
     CDS             complement(1933..>3976)
                     /locus_tag="VFV61_00930"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX4899060.1"
                     /translation="ILRIAAVSDAQISPNGEWVVYAVSTTEGDSIRSTLWLARMPSDV
                     DRSQPTTRPAPTMRGAEQARDWPDVRRPATQLLPASWNASTPRWSPDGSQIAFMASHE
                     GQTGIWVTDPNRSAPKLIAQVQSTNFFITYAGEVFDWSPDSRRIAYISATEEVKEISP
                     TAKPNDPRVIDRIQYKSRTSFSDNKRTHVWIVDIDRPEPRQLTSGPFYDHAVSFSSNG
                     EEIAYLSNHEADPDANNNSDIFAVDLHGEVRQITATSGCEYDPTWSPDNKSIAYLATK
                     RDVTTIDSVAEDTHVWVIDAAGGRGRELATKLDRRARSPRWDGSSREIFFLAGDEGET
                     KLWVTKSDGDDFQEVFPHPKSGETRLERHWQGGRFQISSFSVSARLLPRLAFVLSDSM
                     HPAEVWLASRTDPPVVQRISKHNDEFTNSLLLTPPGVVRFKSFDATSIQGWLMKPLNL
                     RSDRKFPLILSIHGGPHGMSGWGFNAAFQVYAARGYGVLFLNPRGSSGYGQKFSDGTL
                     GEWGGGDYKDLMAGVDEVLRRVAWINAERIGVTGGSYGGFMTNWIITQTPRFKAAVAS
                     ASLSNLISFYSTSLYQDLIHAEFGGFPWDNFELLWQWSPLRYVKQVQTPTLFLHGEQD
                     NDVHITQAEEMYMALKRRGVETVLVRYPREGHGLREPQHRVDALERTLTWFDRFLK"
BASE COUNT          927 a         1100 c         1044 g          905 t
ORIGIN      
        1 gcttcgcctt gaagcttcag ttcttcaccc tgcattccag cctcaccacc gctgcgcccg
       61 ctgaagcgga atccagttga gtcggcaagc aaatcgaatc cgataaagct attaccactt
      121 cgtaatgctc ccaaaaccga ctccgggttg aatccgtccc gttccgagag taaagcgtgg
      181 actcgaacga ttccgaaact tctttcatag ggatccagca gcagtcctat taatgttttg
      241 cccgatgcat cttgcaagcc gatgccgata ttcgcgtgag catcaacccc tgcaagcgcc
      301 gttaggcgct gcccacgggc aagggtctgg tcccacaatc gtaggctctc gttagggcgc
      361 cggtagaaat tcgcaaacag cagctccgga tagctgcggt aacaccacaa tccatcaaag
      421 aacatcatta ggggattgat ctgctgcgca ttggtgtaga ggttgtacac ttcaatgccg
      481 tcataagcgt tggtgttcca accctcgaat tcttccgggt aagcaacaat cgacaagccg
      541 agctttgccc ttacctgctg tgccagatct tcaagactcg cgtcttctgc agcttgcgtt
      601 tccaggccgg gaatgagtag gacgcgaccg gcgtctttag cacttacttc gttgccattg
      661 agaaagagga tgccgccatg aactccgtgc agcgtcatag cggcagtgtt gatatcacgt
      721 tcggtatgct ccgtcatgac gacgaagtcc aactggttcg tttttgcggc agcaatgatc
      781 tcctgaaagg taccgctgct gtgcccgccg aggaacgaat gaacgtggac gacacccttg
      841 tattcgcgat attcactaaa acctggatca acagaagagg tgtttcgttc ggcattcagt
      901 tgttgaatag ttgcctgaag gcgcactagc ctgcgacgcc gataagcgaa tggcagctgc
      961 gacagcagaa tgagaagaac gactacaaag acgatttttt gccgacgctg catcttagag
     1021 ttttgagtgt tgagtgacag gtttcgagtt tgaggggcca gcaaacggag cctcggcgac
     1081 cggatctagg tttcgaaatc aaccgaagca atctgttcag taatcgctcc gccgaaggca
     1141 acaactttta cgtgttcagg atggactgtg taggtctgga gcgccgcccg atctcgaaaa
     1201 acagcaacca gtcccgtgtg ataggagcgt ggcagtttca agacatcaaa ccccactgca
     1261 agactttcga tcacggggat agccgcagcc agactgcgaa gcatactcaa atgcgtttcg
     1321 cgcacttcgg cctctacatc gtcgcgatat ttccaaatta caatgtgggt caacataact
     1381 agtagacagt agtcagtatt tcgcagactc agcttcacat gacgctcgag tcacttgatg
     1441 gcggtagtca aatagttttc gtaaacgccg ctcgattagt attggcgcga acagtcggcc
     1501 caaagcgccg agtggtggtt catattcgat ctcatcccgc aaagtcgccc cttcaggatc
     1561 agctgcgatg atgtggcgat gtttccaact gcgaaagggc cccttgattt gcacatcttc
     1621 aaaaaaattt ggcggttcgt agcgggtatg ttcggctacc caacgcgtct tcagcggacc
     1681 aatgaagttc gtttcgatga tggcgcgcga gcccacatct gcaatcctgg cgcgctgcag
     1741 cacccgcgca gtttcccagg ggggcattag caattccaac gcattgtccc gttcatggaa
     1801 agcgaagacg cgatttggat ttgcgtgaat cagactttcc ttgacgaaga gcattgggtt
     1861 gtccgttgtc cgtggtcagt tgtccggaat ccggcaacta ctgaccactg cctactgcct
     1921 actgtctcct ctctacttta ggaaccgatc aaaccacgtc aaggtccgtt ccaacgcgtc
     1981 aactcgatgt tgtggttccc gcagcccgtg cccttcgcgc ggatagcgca ccaggactgt
     2041 ctccacgccg cgacgcttca acgccatgta catctcttcg gcttgcgtga tgtgcacgtc
     2101 attatcctgc tcaccatgga gaaatagcgt cggtgtttgc acctgcttca cataacgcag
     2161 aggtgaccat tgccaaagta gttcgaaatt gtcccaggga aaacctccaa actccgcatg
     2221 tatcagatcc tgatagagcg aagtggaata aaagctgatt aggttactga gactcgcact
     2281 tgctaccgcg gccttgaatc gcggagtttg cgtgatgatc cagttcgtca taaagccgcc
     2341 atacgaacca cccgtaacgc cgatgcgctc cgcattaatc caggccactc gccgcagaac
     2401 ttcgtcgaca cccgccatca aatccttgta atcgccgcca ccccattcgc ccagtgtgcc
     2461 atccgaaaac ttttgcccat aaccgctcga accacgggga ttgagaaaga gcacgccata
     2521 gccgcgcgcc gcataaacct ggaacgcggc gttgaagccc cagcccgaca taccgtgcgg
     2581 ccctccgtga atactcaaaa tcaaaggaaa cttccggtcc gaccgcagat tgagaggttt
     2641 catcaaccat ccctggattg aagtagcgtc gaagcttttg aatctgacca cgcccggagg
     2701 agttagtagc agactgtttg tgaactcatc gttgtgctta cttattcgct gcaccaccgg
     2761 cggatctgtc cgagacgcaa gccaaacttc tgccggatgc attgagtcac tcaagacaaa
     2821 agcgaggcgc ggcagcaaac gagcactcac actgaagctc gaaatctgaa accgcccgcc
     2881 ctgccaatga cgctccaatc ttgtctcacc cgacttggga tggggaaaga cttcttgaaa
     2941 gtcgtcacca tcacttttgg tgacccaaag tttggtttca ccttcgtctc ccgctagaaa
     3001 aaagatctca cggctggatc catcccagcg tggactccgg gcacgacggt ctagtttggt
     3061 cgctaattcc cggccacggc cgccagccgc gtcaatcacc cagacgtgag tgtcttcggc
     3121 aacactgtcg atggtcgtta cgtcacgctt ggtggccagg taagcaattg acttgttgtc
     3181 cggggaccag gtgggatcat attcgcagcc actcgtagct gtgatttgcc gcacttcgcc
     3241 gtgcagatcc accgcaaaaa tatcggaatt gttattcgcg tccgggtcag cctcgtgatt
     3301 tgaaaggtag gcgatctcct caccattcga actgaaagag acggcgtgat cgtagaaagg
     3361 ccctgacgtt aactgccttg gctctggtcg atcgatgtca acaatccaaa cgtgggtgcg
     3421 tttgttatcg gagaaggacg ttcgcgattt gtactggata cgatcaatta ctcgcggatc
     3481 gttcggcttc gcagtgggtg aaatttcctt cacttcctcg gtcgcagaga tgtaggcgat
     3541 gcggcgagag tcaggcgacc aatcgaagac ctcgccggca taggtgataa agaagttagt
     3601 gctttggacc tgggcgatta acttgggggc agacctgttt ggatcagtca cccagattcc
     3661 ggtttgtccc tcgtgagaag ccatgaaggc gatctgtgaa ccatcgggtg accaacgtgg
     3721 cgtcgaggcg ttccaactcg cgggaagcag ttgtgtcgcc gggcgccgca cgtcgggcca
     3781 atcacgagct tgctcggcac cgcgcatggt tggagcaggg cgagtcgttg gctggctgcg
     3841 atccacgtca gagggcatgc gtgcgagcca gagggtcgaa cgaatcgaat caccctccgt
     3901 cgtggaaacc gcataaacaa cccattcgcc gttgggagaa atctgggcat cgctaacggc
     3961 agcgatgcgt aagatg
//
LOCUS       DASUEX010000052         2092 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_127575, whole genome shotgun sequence.
ACCESSION   DASUEX010000052 DASUEX010000000
VERSION     DASUEX010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127575"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..737
                     /locus_tag="VFV61_00935"
     CDS             <1..737
                     /locus_tag="VFV61_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899061.1"
                     /translation="TLRPREPMSRAHVLRTISRLLQGRSLLQLQKGTTKPANNGLFIL
                     RSNKGKDLPIQVRADAYLFRQLGGEVYQVSSVALVGGESVTFHVNSAGELDYLEVQPA
                     ANGASAERVSPFTNWTRELSLGQIQARLARYARGIGAITDVRVAARGTSRRAIDLELI
                     GTAGTGHVRGGRIRSVLGLREQLFVIDRRLDAAGNVTGFVFTGRGWGHGVGLCQVGAY
                     GLARQGLTYAQILKAYYTGIELTKLY"
     gene            825..1409
                     /locus_tag="VFV61_00940"
     CDS             825..1409
                     /locus_tag="VFV61_00940"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX4899062.1"
                     /translation="MNTEETSGVSNTKPLLTVAYLETLPEDNNRYELIAGELLVSCAP
                     GLPHQLVLQRLQVALVNYLEANPIGIVAPGAGAVFSDYDSVIPDLVFVRNDRWDKIVA
                     NDRFVAAPNLVIEVVSPGPENRKRDFKAKRELYGHFGVEEYWIVDREERSVLIFRLRG
                     QTLEETAVLHESDELTSLLLSGFAINVGSLFDLP"
     gene            1606..>2092
                     /gene="miaA"
                     /locus_tag="VFV61_00945"
     CDS             1606..>2092
                     /gene="miaA"
                     /locus_tag="VFV61_00945"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEX4899063.1"
                     /translation="MSAASSQKPILAVVGPTASGKSTLAIDLALALGGEVINCDSVQV
                     YQGIQIATAKVPLAERRGVPHHLIDFVPPEVNYTAGDWAREAAAKIEEIEARGQIPIL
                     VGGTGLYLRALRRPFFASPPTDENLRRRLTTMRERHGAEYLHRLLQRLDPSSALKLYP
                     RD"
BASE COUNT          451 a          592 c          572 g          477 t
ORIGIN      
        1 ctacgctgcg accgcgagaa ccaatgtcgc gtgcccatgt gttgcgcacc atctcacgtc
       61 tgcttcaggg acgttcactg ttgcagttac aaaagggtac gacgaaacca gcaaacaacg
      121 gtctattcat cctgcgttcg aataaaggca aagacctgcc gatccaggtt cgcgcggatg
      181 cttatctgtt ccgacagttg ggtggtgagg tctatcaagt gagttcagtc gcattggtgg
      241 gtggagaatc agtcactttc catgtcaaca gcgctgggga actcgactac ctcgaagtgc
      301 agccggcagc aaacggcgcg tcagcggaaa gagtctcacc gttcacaaac tggaccagag
      361 agttgtcact gggccagatt caggcgcgcc tggcccgcta tgcgcgcggc attggcgcga
      421 tcaccgacgt gcgtgtggct gcgcgtggca cttcgcgacg cgcaatcgac ctcgagttaa
      481 tcggcactgc cggcaccggt cacgttcgcg gcggtcgcat ccgctccgtg ctcgggttac
      541 gggaacagtt gttcgtcatc gatcggagac ttgatgccgc gggcaacgta acgggtttcg
      601 ttttcaccgg gcggggttgg ggacacggag ttggactctg tcaggtcgga gcttacggct
      661 tggccaggca aggcctcacc tatgcgcaaa tcctcaaggc ctactacacc ggaatcgagc
      721 tcacgaagct ctactgaatg caaagacgct gttactgaat actcaagccc acttccgcgc
      781 cattggttaa ttgagtccta aagccgtgtt agagtcacga tttcatgaac actgaagaaa
      841 ccagtggcgt ttcgaatacc aagccgctcc tcacagtggc ctacctagag acgctgccag
      901 aagacaataa tcgctatgag ctaatcgccg gagaactcct cgtgtcttgc gcacccggtt
      961 tacctcacca actggtactg caaagattgc aagtggcact tgtaaactat ttggaagcga
     1021 acccaatcgg gattgtcgcg ccaggtgccg gtgcggtgtt tagcgactac gactcagtaa
     1081 ttcccgatct tgtgttcgtg cggaacgaca gatgggacaa gatcgtcgct aatgatcgct
     1141 ttgtcgctgc tccgaatcta gttatcgagg tggtttcccc gggcccggaa aaccggaagc
     1201 gtgatttcaa agcaaagcga gagctttacg ggcacttcgg cgtggaagaa tattggattg
     1261 tggatcgcga agaacgttct gttctgatct ttcgattgcg tgggcaaacg ctggaggaaa
     1321 ccgctgtgct gcacgaaagc gacgaattga cctcgctgct actttccggt tttgccatca
     1381 acgtagggtc tttgtttgat cttccatgaa gattcggtac cccgcaccgt caactagtct
     1441 ctgacatccg gtgcgcgcaa gaagaacaaa cctgggacct gagacctgaa acttgaaacc
     1501 cttagttctc cgattgcgct tctcacgctc acaactcatc ggcgccctcg tggtactggc
     1561 cctcctttgg ttagtaatcc ttttccgcat gctatcctct cgcccgtgag tgccgcgagt
     1621 tcgcaaaagc ccatccttgc agttgttggt cccaccgctt ctggaaaaag tactctcgca
     1681 atcgatctcg cattggcact cggcggcgag gtcataaact gcgactccgt tcaggtttac
     1741 cagggaattc aaatcgccac tgcaaaggtt ccgctcgccg aacggcgtgg cgtaccccat
     1801 cacctcattg attttgttcc gcctgaagtt aactacacag ctggcgactg ggcgcgtgaa
     1861 gcggcagcca agatcgagga gatcgaagcc cgcggtcaga ttccaatact tgtcggcggc
     1921 accggattgt atttgcgcgc cttacgccgc cccttctttg ccagcccgcc gacagatgag
     1981 aatctgcgcc ggcgtcttac gacaatgcgg gagcgtcatg gtgcggaata tctgcatcgc
     2041 ctcttgcagc gcctggatcc ctcgtcggcg ctgaaactct atccgcgcga ct
//
LOCUS       DASUEX010000053         3045 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_929918, whole genome shotgun sequence.
ACCESSION   DASUEX010000053 DASUEX010000000
VERSION     DASUEX010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_929918"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1037
                     /locus_tag="VFV61_00950"
     CDS             <1..1037
                     /locus_tag="VFV61_00950"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX4899064.1"
                     /translation="ELFIFLGRVKMDYRFMGRTGLKVSEICLGCQTFGWGADEPASHA
                     LADRFVAAGGNFFDTSNIYNEGKSEMILGSWLKNRRDRAQLVIASKVFFPTGQGPNDT
                     GLSRKHILEQIDISLRRLHTDYLDLYQMHCWDASTPLEETLRTLDDAVRMGKVRYIGA
                     SNYTPAQLTRANLLSEMHGWVRFDCLQPEYSLLVRSTEWELLPLCISEGIGVIAWSPL
                     AGGWLTGKYQRGQPPPPDSRVGRADRWDDQPEQRESELTWRIVDALAEVNKECGKSPS
                     QVALNWLLHQPGLTAPIIGARTLAQLEDNLGSVGWSLGPEQMAKLNAASDIPLPSPYD
                     FIARYTRKRV"
     gene            1048..2070
                     /locus_tag="VFV61_00955"
     CDS             1048..2070
                     /locus_tag="VFV61_00955"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX4899065.1"
                     /translation="MRALWGKAPLQFDMREAPEPIPDFGDVLVRIGGCGICGTDLHFL
                     RAMNRDWSPLGHELAGQVEKVGAGISHLRAGDAVVIENHTGCGVCEACKNGESRYCRS
                     IWTFMNDQAGFGDYICVPKGSVQKFNSLTSRQASLAEPLTVAIDVTLRAEIPLNGEVA
                     VWGAGPIGIMACQIAKLRGARRVFLIGSPRDNPRNRFRLELAGEYGADLAISGGANAV
                     DEIKHVTGDGVDRVLMTAPPAAIPDALRACRFGGIVALIGLEERAGETIMLDINAFHF
                     QKLQLRASHAIPNHYFPIALDLLARRVIDPDRIITHVFPLNEYRRAFETANNPNERVC
                     KVVIEP"
     gene            2093..2674
                     /locus_tag="VFV61_00960"
     CDS             2093..2674
                     /locus_tag="VFV61_00960"
                     /inference="COORDINATES: protein motif:HMM:NF018288.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEX4899066.1"
                     /translation="MKQRPFTFSISVPDENLSEYDNHIVRRLSAMQGQYLDAQAFEAM
                     LTQDPVLYEVYEIKRPELAGELMNGITIVHPGKVGEEYFMTKGHFHTVLDTAEVYYCL
                     KGEGAMVMETPQGEWSVEELHSGRVLYVPPRWAHRSVNTSLREDLVMFFIYPANAGHD
                     YGTIEQQGFRKLILERDSRVQIVDNPRWHPRER"
     gene            2781..>3045
                     /locus_tag="VFV61_00965"
     CDS             2781..>3045
                     /locus_tag="VFV61_00965"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899067.1"
                     /translation="MIYNPFDRPLTSTEVGALLPGCDGYIAGLDYIDRAALDQADQLQ
                     VIARYGTGLDRIDLAAAAEKSIVITNTPFANSVSVAELTIGMIL"
BASE COUNT          679 a          832 c          875 g          659 t
ORIGIN      
        1 atgagctatt catctttctt gggagggtaa agatggacta tcgctttatg ggaaggaccg
       61 gactcaaggt ctcggaaatc tgtctgggct gccagacctt tggttggggg gcagacgaac
      121 cagcctcaca tgccctagcg gaccgctttg tcgcggcagg cggtaacttt ttcgatacat
      181 cgaacattta taacgaaggc aagtccgaga tgatactcgg ttcctggctc aagaatcgta
      241 gggaccgcgc tcagctcgtg atcgcctcca aagtgttctt tcccaccggg cagggaccca
      301 acgacactgg attgtctcga aagcatattc tcgagcaaat tgatatcagt ctccgccgtc
      361 tgcataccga ttacctcgat ttgtaccaga tgcattgttg ggatgcctca acgccgttgg
      421 aagagacgct ccggactctg gatgatgcgg tacgcatggg caaagtgcgc tacattggag
      481 catccaacta cacaccagcg cagctcacgc gcgcgaatct gctcagcgag atgcacggct
      541 gggtccgctt cgactgcctg cagccggaat acagcttgtt ggtacggagc accgaatggg
      601 agttattgcc gctttgcatc agcgagggaa ttggtgtgat cgcttggtcg ccactggccg
      661 gcggttggct gaccggcaag taccagcgcg ggcagccgcc cccgccagac agccgcgtgg
      721 gacgcgcgga tcgctgggac gaccagcctg agcaacgaga aagcgagctt acctggcgta
      781 tcgttgatgc cttagctgaa gtcaacaagg agtgtggtaa atcaccgagc caggttgcac
      841 tgaactggtt attgcatcaa cccggattga cagcccccat catcggagcg cgtacgctgg
      901 cgcaactgga agacaaccta gggagcgtgg gttggtcgct gggcccagag caaatggcga
      961 agctgaacgc agcaagcgac attcctctcc cttctcccta tgatttcata gcaagataca
     1021 cgcgcaagcg cgtctgagag gtgatagatg cgagctttat ggggcaaagc gccacttcag
     1081 tttgatatgc gcgaggcgcc tgagcccatt cccgactttg gagatgtatt ggtgcgaatt
     1141 ggcgggtgcg gaatctgtgg aactgatctg cactttttac gcgctatgaa tcgtgattgg
     1201 tcaccactgg gacacgagct cgccgggcag gtcgagaaag tcggcgcggg tattagccat
     1261 ctgcgcgccg gcgatgcagt ggttattgag aaccatacgg ggtgcggcgt ttgcgaagcc
     1321 tgcaagaatg gcgaaagccg ctattgtcgc agcatctgga cgtttatgaa tgatcaggcc
     1381 gggtttggcg attatatctg tgtcccaaaa ggctccgtac aaaaatttaa cagcctcacc
     1441 tcgcgccagg ctagccttgc agagccgcta accgtcgcga tcgacgtgac tttgcgcgcc
     1501 gaaattccgc tcaatgggga ggtggcagtc tggggcgcgg gccctatcgg gattatggcg
     1561 tgccagatcg caaaactgcg cggcgcacgg cgtgtgtttc tcattggctc gccacgcgac
     1621 aacccgcgca accgtttccg gctcgagctt gccggcgagt atggcgcaga tctggcgatc
     1681 agcggaggcg cgaatgctgt ggacgaaatc aagcacgtca ccggtgatgg tgtggatcgt
     1741 gtgctgatga ccgcgccgcc tgccgcaatc cccgacgcgc tgcgcgcttg tcgctttggg
     1801 ggaatcgttg ccttgattgg cctagaagag agagccggcg agacaatcat gctggacatc
     1861 aatgccttcc atttccagaa acttcagctg cgcgcttcgc acgcgatccc caatcactat
     1921 tttccgatcg cactcgattt gctcgctcgc cgcgtgatcg acccagatcg aataatcaca
     1981 cacgttttcc cgctgaacga atatcgccgt gcgtttgaaa cggcgaataa cccgaatgaa
     2041 cgcgtctgca aagtggtgat tgaaccatag ttggcagagt cagagttgat ccatgaaaca
     2101 acgccccttc actttctcca tttcagtccc ggatgagaac ctgtccgaat atgataacca
     2161 catcgttcga cgcctttcag ccatgcaagg ccaatatctt gatgcgcagg catttgaggc
     2221 gatgctcacc caggatccgg tgctgtacga agtgtatgaa atcaagcgtc ccgaactggc
     2281 gggcgagctg atgaatggca tcacgattgt tcatccaggc aaagtcggtg aagagtactt
     2341 tatgaccaag ggacattttc atacggtact cgacactgcc gaagtctatt attgcttgaa
     2401 aggcgagggt gcaatggtga tggaaacacc gcagggcgag tggtcagtgg aagaactcca
     2461 ctccggtcgc gtgctttacg taccaccgcg ctgggcgcat cgctccgtca acacgagtct
     2521 gcgtgaagat ctggtcatgt tcttcatcta cccggcaaat gccgggcacg attacggaac
     2581 gattgagcag cagggctttc gcaaactgat tttggaacga gatagtcgtg tgcaaatcgt
     2641 agataaccca cgttggcacc cacgcgaaag gtaggtgagt gattcacaac ctcaaatccc
     2701 gtcgagtcct cgtgacgccg acgtcttatg gtaagaatga tccgcagctc cgcagcgagc
     2761 tagaagccac cgtcggtgag gtgatctaca acccctttga ccgccctctc acttcgacag
     2821 aggtaggggc gctactccct ggatgtgacg gatacatcgc cggtttggat tacatcgacc
     2881 gcgccgcgtt ggatcaggcg gaccagctcc aggtcattgc ccgctatggc accggcctag
     2941 accggatcga cctagcagcc gcagccgaaa aatcgatcgt catcaccaat actcccttcg
     3001 ccaattcggt ctcggtagct gagctgacca ttggcatgat ccttg
//
LOCUS       DASUEX010000054         2376 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_235247, whole genome shotgun sequence.
ACCESSION   DASUEX010000054 DASUEX010000000
VERSION     DASUEX010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235247"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="VFV61_00970"
     CDS             complement(<1..265)
                     /locus_tag="VFV61_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899068.1"
                     /translation="MRECLDEAILQSYFDGELSGHHLEGVTSHLAACNTCAVTAREIE
                     AETALLAAAFAPEFEAAVPTEQLRQRIDAAIAGLQTQGLAVNES"
     gene            complement(262..831)
                     /locus_tag="VFV61_00975"
     CDS             complement(262..831)
                     /locus_tag="VFV61_00975"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX4899069.1"
                     /translation="MTGQARALTEPMAKEMATAATALSFDEAFTLHHRAVFRTARSVV
                     RDSALAEDVTQEVFLRLYRNLDATPGEDLLRAWLLRVTLNVARNTLRGQSRSMARDNE
                     YHRETMHDWFAPAPEEDYERRVAIEGARRALEKIKEPMRSCLLLKQQGLSYKEIAVTL
                     SLKETNVGSLVARGRKEFARVYGKIGGSR"
     gene            1260..1658
                     /locus_tag="VFV61_00980"
     CDS             1260..1658
                     /locus_tag="VFV61_00980"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX4899070.1"
                     /translation="MSGESTASHSKLEIEQELRQMNDDWVKALVRADRGTLERIMADD
                     FCFAYPMEGDDKSQFINDVISGDVRVESLARENVSVRIWGDTAVLTGKDSASWSYKGR
                     DFSGHYKIIHVYSRRNSHWQLVSVQACPMS"
     gene            complement(1669..>2376)
                     /locus_tag="VFV61_00985"
     CDS             complement(1669..>2376)
                     /locus_tag="VFV61_00985"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX4899071.1"
                     /translation="QVFSKRGHVFIDGFELSTRFPFGFVRRRRRLGARNVDILVYPKP
                     EPIGDELHLLPMNVGRMASARRGAGHDLFALRDYQPQDDLRHIDWKATARSRRLTVRE
                     FTSEDERRITIVLDTRPVPDSQDESFSTRFERGVVKAASHLKHFIDEQAEVRLVLGDE
                     PGAYGSGLEHLYRNLRRLAVVGPANGAARAAAASVSEEQAFDDADSGHDYAIFLTPAA
                     PGSIPAKIWRSSYVVYF"
BASE COUNT          537 a          685 c          644 g          510 t
ORIGIN      
        1 acgattcgtt aacggccaag ccttgcgttt gcagaccagc aatcgcggcg tcaatgcgct
       61 ggcgcaattg ttcggtgggc actgcggctt cgaactcggg ggcgaaagcg gccgccagta
      121 acgctgtctc tgcctcaatc tcgcgcgctg tcaccgcgca ggtattgcag gcagccaagt
      181 gtgacgtgac gccttccaga tggtggccgg aaagctcccc gtcaaagtag ctttgcagaa
      241 ttgcttcgtc caaacactct ctcatcgact tcctccgatt tttccgtaaa ccctcgcaaa
      301 ttcttttcgt ccgcgcgcaa ccaggctgcc gacattcgtc tctttcaaag acaaggtcac
      361 ggcaatctcc ttataagaga gcccttgctg tttcaaaagc aaacaactgc gcatcggttc
      421 tttgatcttt tccagcgcgc gccgggcgcc ttcaatcgcc acgcgtcgct cgtaatcttc
      481 ttcaggcgcg ggtgcaaacc agtcgtgcat cgtttctcgg tgatactcat tatctcgcgc
      541 catcgatcgg ctctgtcccc gcaaagtgtt gcgcgccaca ttcagcgtta cacgcaaaag
      601 ccacgccctc aaaagatcct cacccggcgt ggcatcgaga ttacggtaga gcctgagaaa
      661 cacttcctgc gtcacatctt cagccagagc cgagtcgcgc accaccgacc gggcagtacg
      721 aaatacagcg cggtgatgaa gcgtaaacgc ctcgtcgaaa ctgagggcgg tcgccgcagt
      781 cgccatttcc ttggccatgg gctcggtcaa agctctagct tgtccagtca ttgatcaccg
      841 ctctcaacca tgcaaacacc gcaaactacg ggtttatggc aactttctta agttgggggt
      901 tgaagccgcc actttcgggg cgcgtccgcg ccgtattgta gtctgaatcg atgtccgcac
      961 aaattcttcg gggagcggat gcaaaaaaca aggggtgact ctctctcaaa cgtaaaacca
     1021 gcctgaaagt tctaaagcct ttcctaccga ttgctaattg ttaattggcg attggcgatt
     1081 gccgattggc gtttagattc caccgagttg taccgcggga gacaaccggc aagaaggaat
     1141 ccggcatttg gcaaagacca atcggcaatt ggcaatcgcc aatcggcaat tctctgggtt
     1201 tgactagccg tgcaggaaag cgtagaaagc gcaggtatcc ctgcacaagg agtgaaagta
     1261 tgagtggcga atcgaccgca tctcattcga agctggaaat cgaacaggag ttgcggcaga
     1321 tgaacgacga ctgggttaag gctctggtgc gcgccgaccg cggcaccttg gaaaggatta
     1381 tggcggacga tttttgtttt gcttatccga tggaaggcga tgacaagtcg cagtttatca
     1441 acgatgtgat ctccggcgat gtgcgcgttg aaagtcttgc tcgcgaaaat gtcagtgtgc
     1501 ggatctgggg cgacaccgcg gtccttaccg gcaaggactc ggcaagctgg tcatacaagg
     1561 gtcgcgactt cagcggtcac tacaaaatta ttcacgtcta ttcccgccgc aacagccact
     1621 ggcaactggt ctcggtacag gcctgcccga tgagttagtt acaccgcctt agaaatacac
     1681 tacataggag ctgcgccaga tttttgccgg tatcgagccc ggcgcggcag gcgtaagaaa
     1741 aatcgcgtag tcgtgtcctg agtccgcatc atcgaaagct tgctcctcag atacagaagc
     1801 agctgcagct cgcgccgcgc cgttggccgg accgacaacc gcgaggcgcc tcaagttgcg
     1861 gtagagatgt tccaggccgg atccgtaagc gcccggctcg tcgccaagaa ccaaacgcac
     1921 ttcagcctgc tcatcaatga agtgcttcag atgtgaagca gctttgacta cacctcgctc
     1981 aaaccttgtg gaaaaacttt cgtcttgtga atccggaacc ggtcgtgtgt ccaggacaat
     2041 ggtaatgcga cgttcgtcct ccgacgtaaa ctcccgcacc gttaagcggc gcgagcgcgc
     2101 ggtcgccttc cagtcgatgt gccgtaggtc gtcctgtggt tgatagtctc gcaaggcaaa
     2161 caagtcgtgg cccgcgccgc gccgcgcgga cgccatccgt cccacgttca tcggcagcag
     2221 gtgcagttca tcgccaatag gctccggttt gggatagacg aggatatcca cgttgcgcgc
     2281 ccccagccgg cgtctccgcc gcacaaagcc aaacgggaaa cgggtggaga gttcaaagcc
     2341 atcgataaac acgtggccgc gcttactaaa aacctg
//
LOCUS       DASUEX010000055         2992 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_586734, whole genome shotgun sequence.
ACCESSION   DASUEX010000055 DASUEX010000000
VERSION     DASUEX010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_586734"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1852
                     /locus_tag="VFV61_00990"
     CDS             <1..1852
                     /locus_tag="VFV61_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899072.1"
                     /translation="AGGAELLTVHARWEGLETSPEQKWIPVVSVLRDTLGDLSPENDR
                     LRYVWPLTYSRPTTWQRISGAVPFLYTRVGNKQTASGPPPPAIDLASPDREVWEKIFW
                     TALQNMLLDPYGAPVKASTRSYRRNISDYRKSQIVRALSVLSLYQSTNGSAAFSEREM
                     AEIQSRLLLTDKTFGGLLDELNLQRYYAKSMVQTRDQRGHNWELLRQRAEAESLYFEP
                     LEMPDGSVTHALLWIAKSDLSSHRDEPYSKRFLNIDKPWGDKRLLNWQGYSETRYFDA
                     DSRPVAPETPGVRAEEMIPLALYGLDNPKIPMLLVDFRDGLNPKKREMSRRLLEDVTR
                     NILSLSRFGDLPYFLGRSVYDFVTGRRGIDLNQPSRLRTYSQLKLLLALNESLDPELR
                     NEITGRLEKVSLNPMENNLEAEAKLAQQQFAALLSYAAKPDGLPAKLERARRSEMVPL
                     EHGKTEQVLFRLANILTFGRYVHREKATPEMESRLDLARRLQFHTNFLREVVRSTPQI
                     DVNWDLEEVKRSLLFIAQHGPNANSQAINATAQIFKRTTDGDTRRACLESLARINKPK
                     ARLELMRISQSKDLDPALRELISGYLNNPNLRLEPLTSSIKANVNRTEQR"
     gene            1858..>2992
                     /locus_tag="VFV61_00995"
     CDS             1858..>2992
                     /locus_tag="VFV61_00995"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX4899073.1"
                     /translation="MTHFRLFQPLIRNLLLCLLLLVPATVFAQAKRVVLVKCDGLSYE
                     AVDSFVRERDPRTGKSQLPWIDYIFYQQGARLKNFYVRGMSLSAPSWSMLETGQHLQI
                     KGNVEFDRYTLHTYDYLNFIPYVVNTTRGVRVDMPGVEVLDSLGEPLLIDAFPHDERY
                     LTFSLFQRGARFITYQKTLENRFKRAPRELFDEWTMGFGMRDALPEQLTREMIEKLSN
                     PDVRYLDLVLTDFDHAAHHNNDRKSHLIALKQIDNALGQIWTAIRKSPLADDTALVVV
                     SDHGFNSDERVYSQGYNLVKLLGSTAGGGHHVITKRRLLLDYAIKGMNPFVSLITTTT
                     RDSFYLKGQSSSYPTALLDFDGNERASIHLRDSDLNLLHILLQQ"
BASE COUNT          686 a          841 c          777 g          688 t
ORIGIN      
        1 tgcaggtggc gcagaattat taacggtcca cgcgcgctgg gaaggacttg agacttctcc
       61 cgaacagaag tggataccgg tcgtcagtgt cctgcgcgat acgctcggag atctcagtcc
      121 ggagaacgac agactccgat acgtttggcc tcttacgtac tcacgaccga caacctggca
      181 gcgaatctcc ggcgcagttc ccttcctcta cactcgagtg ggaaataaac agactgccag
      241 tggtccgcca ccaccggcaa tcgaccttgc ttctccggac cgggaagtct gggaaaagat
      301 cttctggacg gcgctccaga acatgctgct tgatccctat ggagcgccgg taaaggcatc
      361 gacccgttca tatcgccgca acatcagtga ctatcgcaag tcgcaaatcg tccgggcgct
      421 gtctgtgctt tcgctttatc agagcacgaa tggctcggct gcgttctcag aaagagagat
      481 ggcagagatc cagtcacgtc ttctgctaac cgacaagaca tttggtggac tgctcgacga
      541 actgaacctg caacggtact acgccaagag catggttcag acccgtgatc agcgcggtca
      601 taattgggag ttattgcggc agcgcgcgga ggcagagtct ctctattttg agcctctcga
      661 aatgccggat ggcagcgtca ctcatgcgct tctgtggatt gcaaaatcgg atttgagctc
      721 ccatcgcgat gagccataca gcaagcgctt tctcaatatc gacaaaccgt ggggcgataa
      781 gcgtctcttg aattggcaag gatattcaga gactcgttac tttgacgccg acagccgccc
      841 agtcgctcct gagactcctg gagttcgtgc ggaggagatg attccgctcg ccctttatgg
      901 tcttgataat ccgaagatac cgatgttgct ggtggatttc cgcgacggcc tcaatccgaa
      961 gaaacgggag atgtcccggc gactcttgga agatgtcacc cgcaacattc tgtcgctctc
     1021 ccgttttggg gaccttccat acttcctcgg acgcagcgtc tacgactttg tcaccggccg
     1081 gcgtggaatc gatctcaacc agccatctcg gctgcgaacc tattcccagc taaaacttct
     1141 cctggcactc aacgaatcgc tcgaccccga attgcgcaac gagatcacgg gccgacttga
     1201 aaaagtctcc ttgaacccga tggagaataa ccttgaagct gaggcaaaac ttgcgcagca
     1261 acagtttgct gcccttcttt cttacgccgc caaaccggac gggcttcctg ccaagctgga
     1321 gagggctcgc aggtctgaga tggttccgct tgagcatggc aagacagagc aagtactctt
     1381 tcgccttgca aacatcctga cctttggcag atacgtccat cgtgaaaaag ccacgcccga
     1441 aatggaatca aggctcgatc tcgctcgccg cctgcaattc cacacaaatt tccttcgtga
     1501 agtcgtgcgc tcaacgccac agatagatgt gaactgggat cttgaagagg ttaagcgttc
     1561 cttgttgttc attgcgcagc atggccctaa cgcaaactcc caggccatca acgccaccgc
     1621 tcagattttc aagcggacca ctgacggcga cacgcggcgc gcttgtcttg agagcctcgc
     1681 tcgaattaat aaaccaaagg cccggcttga gttgatgaga atatcccaga gcaaagattt
     1741 ggatccggcg ctgcgagaac taatcagcgg ttatctgaac aatcccaatc tgcggttgga
     1801 acctttgacc tcaagcatca aagccaacgt taaccgaact gagcagcggt agcgagcatg
     1861 acccacttcc gcctctttca gccgctaatc aggaatctgc ttctttgcct gctgttgctg
     1921 gttccggcaa ccgtctttgc acaagccaag cgtgtggttc tggtcaagtg tgatggccta
     1981 tcgtatgaag cggtcgacag ttttgtgcgt gaacgcgatc cgcgcaccgg aaagagtcaa
     2041 ctgccctgga tcgactacat cttctatcag cagggtgcgc gactgaagaa cttttatgtt
     2101 cgaggcatga gtctctcagc gccctcctgg tccatgctgg agacagggca acacctgcag
     2161 attaaaggca acgtagagtt tgaccggtat acgttgcaca cctacgacta cctgaacttt
     2221 attccttatg tggttaacac cacgcgtggt gtccgcgtgg atatgcccgg cgtagaggtg
     2281 ctggattccc tcggcgagcc tctcttgatc gatgcatttc cacacgacga gcgctatcta
     2341 actttctctc tgtttcagcg cggtgctcgc ttcattacct atcagaagac tctggagaac
     2401 cggttcaagc gagcaccgcg ggaattgttt gacgagtgga ccatgggttt tggcatgcgc
     2461 gacgccttgc cggagcagct tacgcgcgaa atgatcgaaa agttgagcaa tccggacgtg
     2521 aggtacctgg atctggtact caccgatttt gatcatgccg cgcatcataa taacgatcgc
     2581 aaatctcatc tcatcgcgct gaagcagatt gataatgcac tgggccaaat ctggaccgcc
     2641 attcggaaga gtcccctggc ggatgacact gccctggtcg tggtctcaga tcacggcttc
     2701 aattccgatg aacgtgttta cagccagggt tataacctgg tgaaactgct ggggagcacg
     2761 gctggaggtg gtcatcatgt gattaccaag cgtcggctgt tgctggacta cgcgatcaaa
     2821 gggatgaacc ccttcgtttc gctgatcacc acaaccaccc gcgactcgtt ctacctcaag
     2881 ggccagagct cgagctatcc cactgccctg ctcgattttg atgggaacga gcgagcttca
     2941 atccatctcc gcgacagtga cctgaatcta ttgcatattc tcttgcagca gc
//
LOCUS       DASUEX010000056         4253 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1044841, whole genome shotgun sequence.
ACCESSION   DASUEX010000056 DASUEX010000000
VERSION     DASUEX010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4253
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1044841"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(196..3318)
                     /locus_tag="VFV61_01000"
     CDS             complement(196..3318)
                     /locus_tag="VFV61_01000"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEX4899074.1"
                     /translation="MKSSKFKLPVQLFALFFCFSCCLTVFGQSSATLQGTVSDPKGAV
                     MPKAKIVVHNQGTGLERTTETDSEGNYQVAALPVGVYRVEVQAPGFQTQVVNDLSIEV
                     ARTVTQNFQLVVGGIEQQVSVVSDAPVIEASTTSVGTVINQKTVQDIPLNGRHFVDLG
                     LLIPGSVTPPQNGFLTAPLRGQGSFAFNTAGNREDTVNFQINGINLNDMVQNQITFQP
                     SINTVQEFKVDNSTFSAEYGRNSGAIVNIATRSGTNDFHGELFEFLRNEALDARNFFS
                     ATKPPFKRNQFGGNLGGPIVRNKAFFFFSYEGLRQRQGLTFNSGVLTATERANVTDPI
                     SRRLLDFIPTVNSGTNRFVGSGTAPVNIDQWTGDVSYAIGDNDRLHGYYAFQRDFRGE
                     PNLQGNTISGFGDTRQSHRQIFTLNETHIFGPMLVNEMRFGFNRINITFTPNNPIEPS
                     SLGINVGHNLPLGIPQIAIGGIALNFGGPAGFPQGRSDTTFVLSDTLNYLRGNHSFKI
                     GGEFRRFYNNNFNLDTGTFTFANVAAFQTGAGNGFAITLGDRSSAIGTGALGLFIQDN
                     YKVKPGLTLELGFRYDWNMTPTERFDRFVVFDPATASLRRVGQDIDEIYNQNNKNFQP
                     RLGIAWDPTGSGKMTVRAAYAITVDQPVTNTVTGPTANPPLATPLSFTGTINLATARA
                     TAGAAGLAPATIDHDFKNAYVQSWNLNVQRELTPSLAVMIGYFGSKGTHLRISRNINQ
                     PINGVRPFIRLSASSPISPNTLLGNITQIGSGGNSSYNALWATATKRLSRNFQFNASY
                     TWSKSIDYNSLNSQGIVVQDSYNIRGDRGLSDFDARHRFVISGLYELPWRGNRLVEGW
                     QLGTIVQWQSGNPINILAGNPLAIAGTSIGAGSGIATFTGVASVRPDLIGNPQILGTP
                     NQWFTNIVCDPRAPAGCPSGAVFALPVTLSGTTNVYHFGSLGRNVLIGPGFKNVDFSV
                     TKNTKLTESVRFQFRAEFFDIFNHANFGQPGRTAQVGSSAFGLISGTRFPTGDSGSSR
                     QVQFAAKFLF"
     gene            complement(3527..>4253)
                     /locus_tag="VFV61_01005"
     CDS             complement(3527..>4253)
                     /locus_tag="VFV61_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899075.1"
                     /translation="DHADLEQAYCLSLFKLIKSNFAEADRLRISPRDFTKLAQNYLNK
                     SQLKAVKVAVIECNEEQIRVFADEITNRLGVAVHPLLLSQLESPNRSTHKILTRADLY
                     TTTHFHFKQVKSLLGSYHKKLLQLRLNPNFVPSIINAARQGSLLMIVSNVNYFTAFSQ
                     SLLNVGTPRSVVERISAVDDSDLDQVRAQALRADTVYVSPICDARIQKLLPPHCQELK
                     MDSTLSNESLEMLEAFLLFRSRL"
BASE COUNT          992 a         1066 c         1199 g          996 t
ORIGIN      
        1 tgctccgcgg gctcatttgt gttcagacaa cttcttgatt gagaaaaccg ctatccgtta
       61 aggcgccgag agcgccaaga acgccaggag tgtttggagt aagagaatgt ttgtccactc
      121 aaccagcttg taagtctcct ggcgtgcttg gcggtcttag cgccttggcg gtttcttaat
      181 aaccgaacct cctctctaaa acaggaactt agctgcaaac tgaacctgtc tcgaacttcc
      241 ggagtctccg gtggggaacc gtgtgccgct aatcaagccg aaggccgagc tgccaacctg
      301 cgctgtgcgg cccggctgtc caaagtttgc gtggttgaaa atgtcgaaga actcggctcg
      361 gaattgaaac cgcaccgatt ccgttagctt ggtgtttttg gtcactgaga agtcgacatt
      421 cttgaatcct ggcccaatca ggacattccg accgagactg ccgaagtgat agacattcgt
      481 tgtacccgaa agggtgaccg gcagcgcaaa gactgccccc gagggacatc cagcgggcgc
      541 tcgcggatca cagactatgt tggtgaacca ctgattgggt gtgccgagaa tttgcggatt
      601 accaatcaga tccggccgca ccgaggcaac gccagtaaag gtcgcgattc cggaaccggc
      661 tccgatgctg gtgcccgcaa ttgccaacgg attaccagca agaatgttaa tgggattacc
      721 tgattgccac tggacgattg ttcctaactg ccagccctca accagccgat taccacgcca
      781 cgggagttcg tagagaccgc tgattacaaa ccggtgacgc gcgtcgaaat ctgaaagccc
      841 acggtcgccg cggatgttgt agctgtcctg caccacgatg ccctgtgagt ttagtgagtt
      901 gtagtcgatg gatttcgacc aggtgtaaga agcattgaat tggaagttac gggagaggcg
      961 cttggtggcg gtcgcccaga gcgcgttgta acttgaatta ccgccgcttc caatctgtgt
     1021 gatatttccg agtaacgtgt tgggtgagat cgggctggac gcggacaagc ggatgaaagg
     1081 tcgcacacca ttaatcggtt gattgatgtt gcgtgagatg cgcaaatggg tgcccttcga
     1141 accaaaatac ccgatcatca ccgccagcga aggcgtgagc tcgcgctgta cgttcaagtt
     1201 ccaggactgc acataggcat tcttgaaatc gtgatcgatg gtagccggcg cgaggccagc
     1261 ggcccctgca gttgcacgag cagttgcgag attgatcgtg ccggtgaacg acaagggagt
     1321 ggcgaggggt ggattggcag tcggacccgt tacagtattc gtcactggct gatccacggt
     1381 tatggcgtaa gcagcacgca cagtcatctt tcctgagccg gtcggatccc aggcaattcc
     1441 caagcgcggc tgaaaattct tattgttctg gttatagatc tcgtcgatgt cctgaccgac
     1501 gcgacgcaat gacgctgtcg ctggatcgaa tacgacaaag cgatcaaacc gctcggttgg
     1561 cgtcatgttc cagtcgtaac ggaagccgag ttccaacgtc aacccgggtt tcaccttgta
     1621 gttgtcctga atgaaaagtc cgagcgcgcc ggtaccgatt gcgcttgagc gatcaccgag
     1681 ggtgatagcg aagccgttac cggcgccggt ttggaaagca gcgacattcg cgaaggtgaa
     1741 ggtgccggta tccaggttga agttattgtt gtagaagcgc cggaattcac ccccaatttt
     1801 gaaggaatga ttaccgcgca ggtaattgag agtgtccgaa agaacgaaag tggtgtcgga
     1861 acgcccctgc ggaaagcctg cgggcccgcc gaagttgagc gcgatgccgc cgatggcaat
     1921 ttggggaatt ccgagtggga ggttgtgacc aacgttgatt cccagcgacg acggctcaat
     1981 gggattgttc ggcgtaaagg taatgttgat gcgattgaaa ccaaagcgca tctcgttaac
     2041 cagcatgggc ccgaagatgt gcgtttcgtt gagggtaaaa atctgccggt gagattggcg
     2101 cgtgtcaccg aatcctgaaa tcgtgttgcc ctggagatta ggttccccac gaaagtcgcg
     2161 ctggaaggca tagtaaccat gcagccgatc attgtctccg atggcatagc taacatcgcc
     2221 tgtccactga tcgatattta ctggcgccgt gccggagccg acgaaccggt tggttccgga
     2281 gttcacggtg ggaataaaat cgagcaaccg gcgcgaaatc ggatcagtaa catttgcacg
     2341 ttcggttgcg gttaagacgc cgctgttgaa ggttagtccc tgccgctgcc gcagtccttc
     2401 gtatgaaaag aagaagaacg ctttgttcct gacgattggc ccgccaagat taccgccaaa
     2461 ctgattgcgc ttgaacggag gttttgtggc cgagaagaag ttacgagcat caagcgcttc
     2521 attgcgcaga aactcaaaaa gctctccatg aaaatcgttg gtgcctgagc gagtggcaat
     2581 gtttacaatc gcgccggagt tgcgcccata ctccgcactg aaagtggaat tatcaacctt
     2641 gaattcctga accgtattaa ttgacggctg gaaagtgatc tggttctgaa ccatatcatt
     2701 caggttaatt ccgtttattt ggaagttgac ggtgtcttcg cgattgcccg cggtgttaaa
     2761 ggcaaatgag ccctgaccac gcaacggcgc cgtcagaaat ccgttctgcg gtggcgtcac
     2821 cgaacctgga atgagtaggc ccaaatccac gaagtggcga ccattcagag gaatgtcctg
     2881 caccgtcttc tggttgatga ccgtgcctac cgatgtggtg gaggcttcga tcaccggcgc
     2941 atcagatacg acactgacct gttgttcaat ccctccgacg acgagttgaa agttttgggt
     3001 aaccgtgcgg gcgacctcga tgctcagatc gttcaccacc tgagtttgga atcccggtgc
     3061 ttgcacttcg acccggtaaa ctcccaccgg tagcgccgcc acctgatagt tgccttcgct
     3121 atcggtttca gtggtccgtt caagtccggt gccctggttg tgcaccacaa tcttcgcttt
     3181 gggcatgacc gcgcctttgg gatctgaaac tgttccttgc agcgtggcac ttgattggcc
     3241 aaagaccgtc aagcaacaac taaagcaaaa gaacagagcg aatagttgaa ctggtagctt
     3301 gaattttgac gacttcatga atgtctcctt gagggcgaac gccagagatg cggggagtga
     3361 taagtgtccg aaccgaggtt ttgtctctca aaaaccccaa cttcgaagcc atttacttat
     3421 tgtgccgctt gcgctgagcc taaagttaac cactaatatt gttcccggta aatggaacaa
     3481 tttcgaaggg cgccgagatc tgttccattt cggaatcggg ctgggattaa agtcgcgagc
     3541 ggaacaacaa gaaggcttcc aacatctcca gcgattcgtt ggagagagtg ctgtccattt
     3601 tcagttcctg gcaatggggc ggcaataact tttgaatccg cgcgtcacag atcggcgaga
     3661 cgtagaccgt gtcggctcgc agtgcttgcg ctcgcacctg gtcgagatca ctgtcgtcca
     3721 cggcgctgat tcgctccaca acagatctcg gtgtgcccac attgaggagg ctctggctaa
     3781 aggcagtgaa gtagttcacg ttggagacga tcatcaacag ggaaccctgg cgggcggcgt
     3841 ttatgatcga gggaacaaaa ttgggattga gacgcaattg caggagcttt ttatggtagc
     3901 ttcccagcaa cgacttcacc tgcttgaagt gaaaatgcgt cgttgtgtag aggtctgcac
     3961 gcgtgaggat cttgtgggtg cttcggttgg gactctccaa ttgacttaac aatagaggat
     4021 gcactgcaac accaagtcga ttggttatct catctgcgaa cactcgaatc tgctcttcat
     4081 tacactcgat cacagctacc ttcaccgcct tgagttgcga cttattcaga tagttttgtg
     4141 cgagcttcgt gaaatctctg ggactaattc tgagccggtc agcttccgcg aagtttgact
     4201 tgatcaactt aaacagcgaa aggcaatagg cctgttccaa atcggcgtgg tcg
//
LOCUS       DASUEX010000057         3958 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1268559, whole genome shotgun sequence.
ACCESSION   DASUEX010000057 DASUEX010000000
VERSION     DASUEX010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3958
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1268559"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..720)
                     /locus_tag="VFV61_01010"
     CDS             complement(94..720)
                     /locus_tag="VFV61_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899076.1"
                     /translation="MENREVGVEDSSAEDGALTLPHFDDEATVQSARPVVPLHEVRKA
                     SRSKRRLILGAALCLSAFVGAVAASLVYSNRAQRAQQSATAASPAAAQESAAGFTTPS
                     GEVSGAIVNPDEAVAVDTEAATATPSDDRPEVQNSTRASRQPVKNTRVPHPVESVVSQ
                     PEPQDREATEEEIRREARRLRRERRINERRAGDGLTRIREIFEGSPRP"
     gene            complement(944..2863)
                     /gene="dxs"
                     /locus_tag="VFV61_01015"
     CDS             complement(944..2863)
                     /gene="dxs"
                     /locus_tag="VFV61_01015"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF003933.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEX4899077.1"
                     /translation="MGLLENINSAADLRRLPIEQLPDVAAEIRQYILETMSRIGGHTG
                     ASLGAIELAVALHYAFDTPRDRLVWDVGHQAYAHKILTGRRDLLPTIKQYGGISGFLR
                     RDESEYDTFGAGHASTSLSAALGMAVARDKKGEQHHVVALIGDASLAGGMAMEAINQA
                     GHLKTRLIVVLNDNEMSIAPAVGALTGYLNRIRTAQGYHRFKDEVEETLLSIPSLGER
                     LHHAAKTVKDAIAAAVLPGALVSELGFKYIGYVDGHNTRALVAALREAKQVKDGPVIV
                     HALTTKGKGHPQAEKDYYRWHATGPFDLQTGKAIKSAAKAPTYTAVFGSTLCQLMESD
                     AQVVALTAAMPDGTGVCEALERFPDRSFDVGIAEQHCVTFAAGMSVEGLKPVCAIYST
                     FLQRGFDQLVHDVCIQNLNVKFCLDRGGIAGGDGPTHHGLLDIAYLRSVPNIIVMAPK
                     DEGEMRDMMLTMLEHNGPAAMRYPRGNGVGADIERQPELLEVGKGEILRDGGEIAIIA
                     YGAMVHPSLRAAENLAKEGIETTVVNARFVKPLDASLLLALARTKRLLVTVEEAYLAG
                     GFGSAILELLEENGLQDKVRVVRMGIPDRLVTHGDPKLLLAKYGLDVDGIYTRVRESI
                     DVLDDRRAKPQKVGS"
     gene            3131..3640
                     /locus_tag="VFV61_01020"
     CDS             3131..3640
                     /locus_tag="VFV61_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899078.1"
                     /translation="MFLRKILLASLAGLALAAITHAQQDQTPRQEKMGSSQEGPREGR
                     RHRRMERHRGFAALRELNLSEEQRQQQRAILQRQLENTKAQREELFKLRENRFAGTLT
                     PEDETRAQTLHREIQDSRQRMRTEMQSVLTAEQLAKLEQLKTERKARREEMREHRRER
                     REHREPTPR"
     gene            complement(3709..>3958)
                     /locus_tag="VFV61_01025"
     CDS             complement(3709..>3958)
                     /locus_tag="VFV61_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899079.1"
                     /translation="ATADVLATRQRWSEAGLITRDEMQTNCCYATQDKTWVHDPDGNE
                     WEVFVVLADNLPETDPCACGDKVQIGTTSQRQTASCCV"
BASE COUNT          879 a         1166 c         1045 g          868 t
ORIGIN      
        1 cttagaaaac ttaaccgcat gctcgcaacc tgaggcgcgt ccggctgaca attgatgttg
       61 ccggattgct tgtggttgaa ctgcaaagcg gctctacggt ctgggggagc cctcaaaaat
      121 ctctctgatc cgcgtaaggc cgtctcccgc gcgacgttcg tttatgcgac gctcacgcct
      181 taagcgtcgc gcctcgcgac gtatctcctc ttcagttgct tcgcgatcct gcggctccgg
      241 ctgactaact acgctttcga ctgggtgggg aacacgagtg ttcttaactg gctgccggct
      301 cgcccgagtt gagttttgta cttcaggtct gtcgtcacta ggagtagccg tggcagcctc
      361 tgtgtccacg gcaactgctt catcgggatt tactatcgcg ccactcactt ctcccgacgg
      421 agtagtaaaa ccagcggcgg attcttgcgc ggccgcgggg ctcgctgccg ttgccgattg
      481 ctgggcccgt tgtgcgcgat tgctgtaaac gagggaagca gctactgctc ccacgaaggc
      541 tgatagacaa agcgccgcgc ctaatatcag tctgcgcttg gatcgcgacg cctttcgcac
      601 ctcgtgaagc ggtacgaccg gacgcgcgga ttgcacggtg gcttcgtcgt cgaagtgcgg
      661 aagcgtgagc gctccgtcct cggccgatga atcctcaact cctacctcgc gattttccat
      721 aaatgctatc cccttgttgc agtctgcaaa cctcaacttc tgcaattgca aggccattga
      781 ccgaatgtga tttctaactg ccttgacagt agcagaaggg ctgatttccg cacgcaaacg
      841 gatcgttttc cagcccttac ggaacagatc gtttccatat ggtgcagaaa aaacgcccgt
      901 tccggtatgg aacgggcgca cctgatgctg tttaaatcag tttctaggaa ccgaccttct
      961 gcggctttgc gcggcggtca tcaagcacat cgatgctttc ccgcacccgc gtgtagatgc
     1021 catcgacgtc caatccgtac ttcgcaagca gtaactttgg atcgccgtgc gtcacgagac
     1081 gatcaggaat gcccatccgc actacgcgaa ctttgtcttg cagcccgttt tcctccagca
     1141 actctaaaat tgccgaacca aaaccgcccg ccaagtaagc ttcttcaact gttaccagaa
     1201 gtcgtttggt tcttgctaag gccaacaaga gcgacgcgtc cagaggctta acaaaccggg
     1261 cgttgaccac tgttgtttca ataccttctt tggcgagatt ctcggctgcg cgcaacgagg
     1321 gatgcaccat cgcgccatac gcgatgatgg caatctcgcc accgtcgcga agtatttcac
     1381 ccttgcccac ctcgagaagc tccggctgac gttcgatgtc agcgcccaca ccatttccgc
     1441 gtggataacg catcgctgcc ggaccattat gctccagcat cgtcaacatc atgtctcgca
     1501 tttcgccctc gtctttgggc gccatcacga tgatgttcgg caccgaacgc aagtaggcaa
     1561 tgtccagcag tccgtgatgg gtcgggccat cgccgccggc aataccaccg cgatcgagac
     1621 agaacttaac gttgaggttt tgaatgcaga catcatgaac cagttgatca aaaccgcgct
     1681 ggaggaaagt tgaatagatg gcgcagacag gtttcagtcc ctctaccgac atgccggcgg
     1741 caaacgtgac gcagtgctgc tccgctatgc ccacatcaaa cgaacggtcc gggaaacgct
     1801 cgagggcttc gcaaactccg gtgccatcag gcattgctgc agtcaaggca acgacctgcg
     1861 catcgctttc cattaactgg caaagggtac tgccaaacac cgcagtgtag gtcggtgcct
     1921 tggctgcaga cttgatagct ttacccgtct gcaaatcaaa aggtcccgtt gcatgccaac
     1981 ggtaatagtc cttttcggcc tgaggatgac ctttgccctt ggtcgttaac gcatggacga
     2041 ttacgggacc gtccttaacc tgttttgctt cccgcagcgc agccacaagt gcacgcgtat
     2101 tgtgtccgtc aacatagccg atgtatttga atcccaactc gctgaccagt gcgcctggca
     2161 atactgctgc agcgatcgcg tccttcaccg ttttcgctgc atgatgcaga cgctcaccga
     2221 gcgaagggat cgataacaac gtctcctcaa cctcatcctt gaagcggtga taaccctgcg
     2281 ccgtgcgaat tcgattcaaa taaccagtca aggcgccgac tgccggagca atcgacattt
     2341 cgttgtcatt caaaacgacg atcaaccgag tcttgagatg tccagcctga ttgattgctt
     2401 ccatcgccat gccaccggcg agcgaggcgt caccgatcag ggcgacaaca tgatgctgtt
     2461 cgcctttctt atcgcgggcg accgccatcc ccagcgcagc ggacagagaa gtcgacgcgt
     2521 ggcctgcccc aaacgtgtcg tactccgact catcgcgtct caggaaccca ctgatgccac
     2581 catattgctt gatcgtcggc agcaaatcgc gacgcccggt gagaatcttg tgcgcataag
     2641 cctggtgacc cacgtcccaa accaaacggt cccggggcgt atcgaacgca tagtgcaagg
     2701 cgacggcaag ctcgatagcg ccgagagaag cgccggtgtg gccaccgatg cgtgacatgg
     2761 tttccaggat gtattggcga atttccgcgg ctacgtccgg aagttgttcg attggtagtc
     2821 gccgcagatc ggcggcggaa ttgatgtttt ccaggagacc catttggcgc attgtaactt
     2881 gctgattctt gttttactac ttcacaaatt tctaaccatt tcgttgttcc accgccagac
     2941 attgcctttc gggcgtcggc gaccgcccat gctttgcgcg gcaaagtact ctgtagcatc
     3001 attgagtccg cgccgtcaag cttttagttg agccatggcg ggagtggccg cgtctaagga
     3061 ggaagaaaag aagtttcgtc gtccccggga ttttgcactc atcgcgacgg aaaaaaggag
     3121 tacagcagaa atgtttttga gaaagatttt gttagcttcg ttggctggac tggcgttggc
     3181 cgccatcact cacgctcagc aagaccaaac cccaaggcaa gagaaaatgg gtagcagcca
     3241 ggaagggccg cgtgagggtc gccggcaccg taggatggaa cgtcacagag gtttcgctgc
     3301 attgcgcgag ttgaacctaa gtgaggaaca acgacaacaa cagcgggcga tcttgcaacg
     3361 ccaattggaa aacacaaagg cacagcgaga ggaactcttt aaactgcgcg aaaacaggtt
     3421 cgctggcact ctgactccag aggatgaaac ccgcgctcag acattacacc gcgagattca
     3481 agactcgagg cagcgcatgc gcactgagat gcaaagtgtt cttactgccg aacagctcgc
     3541 caaactcgag caacttaaga cagaacgtaa ggcgcgccgg gaggaaatgc gcgagcaccg
     3601 acgcgagcgg cgcgaacatc gcgagccgac gccacgataa actcttcgga ttcctcgccc
     3661 cccagggccc ggccttctca aaaggtcggg ccttttgctt gccccgtctc aaacgcaaca
     3721 agacgcagtt tggcgctgac ttgtcgtgcc gatctgcact ttgtcaccac acgcacaggg
     3781 atctgtctcc ggcaagttgt cagccagcac cacaaatact tcccactcgt tcccgtccgg
     3841 atcgtgcacc cacgtcttgt cttgagtcgc atagcaacaa ttggtctgca tttcgtcgcg
     3901 agtgatcaaa cctgcttcag accaacgttg ccgggtggcc aacacgtcgg ccgtcgca
//
LOCUS       DASUEX010000058         2235 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_701970, whole genome shotgun sequence.
ACCESSION   DASUEX010000058 DASUEX010000000
VERSION     DASUEX010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_701970"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(80..643)
                     /gene="rsmD"
                     /locus_tag="VFV61_01030"
     CDS             complement(80..643)
                     /gene="rsmD"
                     /locus_tag="VFV61_01030"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEX4899080.1"
                     /translation="MRVIAGKYRGRNLKSPPSLQVRPTSDRLRETLFNVIAPRITGAR
                     FLDLCAGSGAVGIEAISRGAAHATFVDRSRKLCALIESNLELCRIPGAESSVVTAEAS
                     EFLRSATKHNREPWDIVFFDPPYADDYGTVLEQLGSQTGDLVKEDGLLIVEHDKRNQL
                     PDRLGLLQRDRVLQQGDSALSFYGRPR"
     gene            complement(658..>2235)
                     /gene="recG"
                     /locus_tag="VFV61_01035"
     CDS             complement(658..>2235)
                     /gene="recG"
                     /locus_tag="VFV61_01035"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEX4899081.1"
                     /translation="EAIPVKEIKETLPAELRQRQKLVGRASALREVHFPPDTADLDEY
                     LSARSAAHRRLIFEDFFWIALGIALKRGGRVKERKGATVKIDAAVKTRIAAVLPFKLT
                     NAQRRVVKQIFADVQSEAPMNRLLQGDVGSGKTIVAVISMLATMESGYQTALMVPTEI
                     LAEQHARNIKKLLARTPYRVELLTGSLKTAEKKRLQAELAAGEIHACIGTHALVQEAV
                     SFKNLALAVIDEQHRFGVLQRAELRARGLNPDVLVMTATPIPRSLAMTVYGDLDVSVI
                     DELPPGRTPIETKVLGEDQRLEVKRLLTREIKAGRQVYVVYPLVEESEKIDLRDATRR
                     YEYLRDVVFPKYRVGLIHGKMKTADKEEVMKRFVAGEIQILVSTTVIEVGVDVPNASV
                     MVVEHAERFGLSQLHQLRGRVGRGAEKSYCLLLTADKKTAVAVERLGIMEQTSDGFLI
                     AEKDLEIRGAGELLGTRQSGLPEFRVANLVRDQQILGQARKEAEFYLAQRERSVETKR
                     MIQRVQSDKRFGLAAVG"
BASE COUNT          466 a          685 c          586 g          498 t
ORIGIN      
        1 accgttcctc gctgatggta cttacttgac actgacactt gaatccggtc cgagccaatc
       61 ggcaatcgcc attgggcaat taccgaggac gtccgtagaa actgagagcc gaatcgccct
      121 gctgcagaac tcgatctcgc tgcagtaacc cgagccgatc agggagctgg ttccttttgt
      181 cgtgctcgac aatcagcaag ccatcttcct tcactagatc tccagtttga gatcctagtt
      241 gttcgagtac agtcccgtag tcgtcggcat aaggcggatc gaagaacacg atgtcccagg
      301 gttcgcgatt gtgttttgtc gcgctgcgga gaaactcaga tgcttcagcc gtaaccacag
      361 aactctctgc gccaggaatc cggcacaact ccagattcga ctctatcaac gcgcacagct
      421 ttcgcgaacg atcaacaaag gtggcatgcg ccgcgccgcg cgaaatcgcc tcaatgccaa
      481 cggccccgga gccagcacaa agatccagga agcgtgctcc ggtaatgcgc ggcgcgatca
      541 cgttgaaaag cgtctctcgc aggcgatccg aggtggggcg cacttgcagc gaaggaggac
      601 tcttcaggtt tcgaccccga tactttcctg cgatcactct catttcaagc ggacacctta
      661 cccaactgcc gccaatccaa atctcttatc gctctgcacc cgttggatca tccttttggt
      721 ttccaccgac cgttctcgtt gcgctagata gaattcggcc tccttgcggg cctgcccgag
      781 gatctgttgg tcgcgcacga gattcgcgac gcgaaactca ggcagcccgg attgtctagt
      841 gccaagtagc tcgccggcgc cacgaatctc caaatccttt tccgcgatca ggaaaccatc
      901 gctcgtctgt tccatgattc ccaggcgttc caccgcaacc gcggtcttct tatccgccgt
      961 cagcagcagg caataacttt tttcagcgcc gcgtcctacg cggccccgca gttgatgaag
     1021 ttgagacaag ccgaatcgtt cggcatgctc aacaaccatc acagaagcgt tgggcacgtc
     1081 gacacccacc tcaatcacag tggtggagac cagaatctgg atttcgccgg cgacaaatcg
     1141 cttcatcacc tcttccttgt cggccgtttt catctttccg tgaatcaagc cgacgcggta
     1201 cttgggaaac accacgtccc gcaagtactc gtatcttctg gtggcatcgc gcaagtctat
     1261 cttctctgac tcctcaacga ggggatagac aacatacacc tggcggcctg ccttgatttc
     1321 gcgcgtcagc aagcgcttga cctcgagccg ctggtcttcg cccaagactt tcgtttcgat
     1381 cggagttcgt cccggtggca gttcatcgat cactgagacg tcgagatctc catagacggt
     1441 catggcgagc gagcgaggaa tcggcgtggc agtcatcaca aggacatcag gattcaaacc
     1501 gcgagcgcgc aactcggcac gctgcagcac gccaaatcgg tgttgttcat caatgacggc
     1561 gagcgccagg ttcttaaacg agacagcttc ctggacgaga gcgtgcgtgc cgatgcaggc
     1621 gtgaatctcc cctgcagcaa gctccgcctg caatcgtttc ttctcggcgg tcttgagtga
     1681 gcccgtcaac agttccacgc gataaggggt gcgggccagc agcttcttga tattgcgggc
     1741 atgttgctcc gccagaattt cggtgggcac catcagcgcc gtttgatatc cgctttccat
     1801 agtcgccagc atcgagatca cggcgacaat tgtcttgcca cttcccacat cgccctgcag
     1861 caggcgattc ataggcgctt ccgactgcac atccgcaaat atttgcttca cgacccgccg
     1921 ctgcgcgtta gttaatttga agggcagcac cgctgcgatt cgcgttttga cagcggcgtc
     1981 gatcttgacg gtggcgccct ttcgttcttt cacgcgcccg ccgcgcttta gagcgattcc
     2041 caacgcgatc caaaagaagt cctcaaagat gaggcgacga tgagcggcgc tgcgagccga
     2101 caggtattca tcgaggtcgg cggtatccgg cgggaagtga acttcccgga gagcgctggc
     2161 acggccaacc agtttctgtc gttgccgtaa ctccgccggt agtgtctcct tgatttcttt
     2221 gacaggaatg gcctc
//
LOCUS       DASUEX010000059         2358 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_816472, whole genome shotgun sequence.
ACCESSION   DASUEX010000059 DASUEX010000000
VERSION     DASUEX010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_816472"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(202..627)
                     /locus_tag="VFV61_01040"
     CDS             complement(202..627)
                     /locus_tag="VFV61_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899082.1"
                     /translation="MSSPYQREEQRVSETVDDLMVRDDQDPNDDSARTASSVRDIASR
                     IPESLSAIGRDISRTIERAMSAKDDYVVAVKISPEAQDKLEQLVQAGVFRNRAEAAGF
                     LIDEGIKTQAALFGRVEQKLAEIERLRAELRGMITEKPI"
     gene            954..>2358
                     /locus_tag="VFV61_01045"
     CDS             954..>2358
                     /locus_tag="VFV61_01045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX4899083.1"
                     /translation="MKKFSLNAAILLLSFTLAIPGLVFAQQKSQSQPPPGSSSAARPT
                     TNVPAPGRRVARGTSSNVIPLIERDFDEALELIKENYVEGKRVDYNAAFKSSIIGMLR
                     SLDPHSNYFDREEFEELKTDQRSEYFGIGASIYSYTVGKDADTFIIATFAEAPANRAG
                     LRFGDRILAVDGVKMTGRPSAEVRDKIRGPRGSVVKITIERALDKRVRTLEITRDAVP
                     QPSIPDSYLLKPGVGYVDMTRGFNYSTTEELQQAVEHLRTKGMTSLVLDLRNNPGGFL
                     DQSINVAELFLPAGQLILTQKGRNGLRDNTYISRNTTPDLIPLVILINESTASASEIV
                     AGAMQDHDRALIVGKTSFGKGLVQSIIPLEFGAGLTLTSAKYYTPSGRLIQRDYSNGG
                     FYDYYTHGGSNRLEQKPEANKPSGPEKKTDTGRAVYGGGGIAPDEVVNAKVISPGQRK
                     LLSPIFSFSRELVNGRVN"
BASE COUNT          528 a          638 c          624 g          568 t
ORIGIN      
        1 ggcacggcgt tatccggtga gaggatcacg cggaggccat tcggaagtgt aaattgttcg
       61 attggtgttt gcgggagttg aaaagtttct gccacgtttg tctcgattcg cctaactctc
      121 ttctgggcgt gctcatctcc ctgagcggga gaattcactt cggttggggg accgaggaaa
      181 ctgctgtttc gcgcctcgct tctaaattgg tttttcagta atcatgcctc gcaattccgc
      241 tcgcagtcgt tcaatttcgg ccaacttttg ctcgacgcgt ccgaagaggg cggcttgggt
      301 cttgatacct tcatcaatca gaaaaccggc agcttcggcg cgattgcgaa aaacgcctgc
      361 ctggacgagt tgctcgagtt tgtcctgggc ttccggagaa atcttgaccg caactacata
      421 gtcatcctta gctgacattg cccgttcaat cgtgcggctg atatctcttc caatcgcgct
      481 caatgactcg ggaatgcgcg aggcgatgtc tcgcactgaa gaagcagtgc gcgcgctgtc
      541 gtcattagga tcctgatcat cgcggaccat cagatcgtcg accgtttcgc tgacacgctg
      601 ttcttccctt tggtatggac tactcatgat taaagactcc ttagatccaa gagagttaaa
      661 ctgtccatgg tattacgtgt ttagccgcgc tgaagttcat tctacggacg ccttcaaagg
      721 cccgtcaagc tggggtacaa agtggcatga tcctggttgc tcgtcttaac cttgttattt
      781 gaagaaagta tcttctctga aaaggtggag gtttgaccgt tacttcagag ccgctcagca
      841 atcgcgaaac gcaaccttgc tcgcgtctga ggcatccgtt agtatggccg tgaacgctgc
      901 atttctggtc agcgtgtcgt gtcggcaaat acctggttga tggtgaaata ttagtgaaaa
      961 agttttcctt aaatgctgcc attctgctcc tcagcttcac gcttgcgatt ccgggtctgg
     1021 ttttcgccca acaaaaatct caatcacagc ctccgccagg cagtagttcg gctgcgcgac
     1081 caacgacgaa tgtacccgct ccaggccgtc gcgtggcgcg gggaacttcc agcaatgtaa
     1141 ttcctttgat cgagcgcgac tttgacgagg cgctggagtt gatcaaggaa aactacgtcg
     1201 aagggaaacg agttgattac aacgcggcat ttaagtcctc gattatcggc atgctgcgtt
     1261 cgctcgatcc gcactcaaat tactttgacc gcgaagagtt tgaagagctg aagacggatc
     1321 aacgttcgga gtatttcggc atcggcgcct cgatttacag ctacacggtg gggaaggatg
     1381 ccgacacctt tattatcgcc acctttgccg aagctcctgc caaccgcgcc ggcttgcgtt
     1441 tcggtgaccg cattctcgca gtcgatggcg tgaaaatgac cggacgccct tcggcggaag
     1501 tcagggacaa gattcgtggc ccccgaggct cggtcgttaa gatcacgatc gaacgcgccc
     1561 tcgataaacg tgtccgcacg ctcgagatta cgcgggatgc ggtaccacaa ccgtcaatac
     1621 ccgacagtta tttgctgaaa cccggtgtgg gttatgtgga catgactcgc ggtttcaact
     1681 actcaacaac tgaagaactg caacaagccg tagaacacct gcgcaccaag ggcatgacat
     1741 cgctggtgct tgacttgcgc aataaccccg gcggtttcct cgatcaatcc attaatgtcg
     1801 ccgaactgtt tctcccggcg ggccagctga ttctcactca gaagggaaga aatggattgc
     1861 gcgacaacac gtacatctcg cgcaatacga cgccggattt gattcccctc gtaatcctga
     1921 tcaacgaaag caccgcgtcg gcttccgaga tcgttgccgg agccatgcag gatcacgatc
     1981 gcgccttgat tgtcggcaag acaagtttcg gaaaagggct ggtgcagagc atcatcccgc
     2041 tggaattcgg cgccggcctg actctgacct ccgcgaagta ttacacgccc tcaggacgcc
     2101 tgattcagcg cgactattcc aatggcggct tttacgacta ctacacacac ggcggctcca
     2161 accggctcga gcagaaacct gaagctaaca agccttctgg acctgagaag aagaccgaca
     2221 ccggccgcgc tgtttatggt ggtggtggga tcgctcccga cgaagtagta aacgccaagg
     2281 tcataagtcc tggtcagaga aagctcttaa gtcccatctt cagtttttct cgcgagctgg
     2341 ttaacggacg cgtcaatg
//
LOCUS       DASUEX010000060         2645 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_472987, whole genome shotgun sequence.
ACCESSION   DASUEX010000060 DASUEX010000000
VERSION     DASUEX010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472987"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..566
                     /locus_tag="VFV61_01050"
     CDS             <1..566
                     /locus_tag="VFV61_01050"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX4899084.1"
                     /translation="TLTSRTANPGEVIDANKELMRVTDLSSVWVVGQVYEKDLGRIRI
                     GSGASITSESFPGRIFRGQVSYVDPTLDTATRTGQVRVELANPGLQLKIGMYVNMAFA
                     TVGGAKSTAATVAKSAVQNINNQQVVFVATKEPNVFVMRPVRLGPEVNGRYQVVDGLT
                     VGESIVSEGSFLLRAEYLKSAVTNHSH"
     gene            710..1201
                     /locus_tag="VFV61_01055"
     CDS             710..1201
                     /locus_tag="VFV61_01055"
                     /inference="COORDINATES: protein motif:HMM:NF025170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYCXC family (seleno)protein"
                     /protein_id="HEX4899085.1"
                     /translation="MNKKITLLLIILALGIVVALLIRSQSATERSAADKTVATESAAE
                     PESHASHTTQPARVPAFQTAAASKDLGPTLGPEKFIGKTREAYRVAKQIPATLAQLPC
                     YCECDRGFGHKSLHSCFEDDHASHCAVCVDEALLAYQLQTQNKMTPEQVRQRIIEKYS
                     EAN"
     gene            1348..2061
                     /locus_tag="VFV61_01060"
     CDS             1348..2061
                     /locus_tag="VFV61_01060"
                     /inference="COORDINATES: protein motif:HMM:NF016269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HEX4899086.1"
                     /translation="MKRSARRRGGISLSVALLLMGLAIATPAQNKSRMQDAARHASDA
                     AKVFTQIMNVRDKAIPKELLDKAEAIAVFPGVIKAAFIFGGRGGQGVISKRQKNGWSA
                     PAFFNLTGGSFGAQIGAQKNDYVFLIMNEDGVKGLLEDKFEMGGEAGIVAGPVGREAA
                     ASTNTTLDAGILSYSRSKGAFIGAALKGVAISPDNDLNQAVYNMKAKEMLMGKPMTIA
                     TMPASVRIFPRTLARYSIR"
     gene            2213..>2645
                     /locus_tag="VFV61_01065"
     CDS             2213..>2645
                     /locus_tag="VFV61_01065"
                     /inference="COORDINATES: protein motif:HMM:NF012920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HEX4899087.1"
                     /translation="MLPRTQRQKEILDYITLFLERHAYEPSYAQIARHFGVKSRATIA
                     KHVAALERRGLLTRKNEEGAFALSVKVDDTDALCEVKLLGRIAAGAPIEVVSDEETIP
                     VPRFLLGRVRPERVFALKVKGDSMIDEHICDGDIALIENRTE"
BASE COUNT          660 a          650 c          763 g          572 t
ORIGIN      
        1 gtacgcttac cagccgcacc gctaaccccg gcgaggtgat cgatgctaat aaagagctga
       61 tgcgggtgac ggatctttcg agcgtttggg ttgtggggca ggtttacgaa aaggaccttg
      121 ggcgaatcag gattggcagt ggcgccagca tcaccagtga gtcttttccg ggtaggatct
      181 ttcgggggca agtttcctac gtggatccca ctctcgatac ggccacccgc actggtcagg
      241 ttcgcgttga actggcaaac cccggcctgc agctgaagat tggcatgtac gtgaacatgg
      301 cgtttgcgac cgtgggtggt gcgaaatcta ctgcggcgac ggtggcaaag agtgccgttc
      361 agaacatcaa taaccagcaa gtggtgtttg tggcaacgaa agagccaaac gtatttgtga
      421 tgcggccagt ccgacttggt cccgaagtca acggtcgcta ccaggtcgtg gacgggttga
      481 cggtaggaga gagcatcgtc agtgagggca gctttctgtt gcgtgctgaa tacctgaagt
      541 ctgctgttac caaccattcg cactgagttc agccgtgtcg cgctgaaaag cgcttgggat
      601 cactttggtc aaaggagatt tgcgtgggat agtaaggacg cgtgtttcgc aaacttgaag
      661 atgagaaacg tcactcgcaa atgtacgcaa agcttttgga gattcgtcta tgaataagaa
      721 gatcactcta ctgcttatta ttctcgccct cggtattgtt gtggccctgc tgataaggtc
      781 gcagagtgcg acagaacgga gcgcggcaga caagactgtt gcaactgaga gcgcagcgga
      841 accggaatcg catgcttcac acacgaccca gccggcgaga gtgcctgcct ttcaaaccgc
      901 agcggcgtcc aaagacctgg ggccaacgct aggacccgaa aaattcatcg gaaagacgcg
      961 cgaagcctat cgagtcgcta aacagattcc cgcgaccctg gcgcagttac cctgctattg
     1021 cgagtgcgat cgcggttttg gacacaagag tctgcattcc tgttttgagg acgatcacgc
     1081 gtctcattgc gctgtttgtg tggacgaagc cttgctcgca taccaactgc aaacgcagaa
     1141 caaaatgacg ccagagcaag tgcgccagcg aattattgag aagtactcgg aagctaatta
     1201 gaaacggaag tggtaccgtg aagttaatcc agatgagcaa actcgcagat gaaagccgaa
     1261 ttatgaatcc aaatgggcta cgctgacatt tgcgatcaga aaatgaagaa agagggactc
     1321 gaaattgtca gaagaaggag tgaagtaatg aaacgatctg caagaagaag gggcggaatt
     1381 agtctaagtg tcgcgttgct gttgatgggt ctcgcgattg cgactccggc acaaaacaaa
     1441 tcgcgcatgc aggatgcggc ccgccatgcc agcgatgccg ctaaggtatt tacccaaatc
     1501 atgaacgtcc gcgataaggc gattcccaag gaattattgg ataaagctga ggcaattgcc
     1561 gtttttcctg gcgtcattaa agctgcattc atcttcgggg gtcggggtgg ccagggagtc
     1621 atcagcaaac gccaaaagaa tgggtggagt gcgccggctt tcttcaatct caccggcggt
     1681 agcttcggcg cccagatcgg cgcccagaaa aacgactacg tgtttctgat catgaatgaa
     1741 gatggcgtca agggtttgtt ggaagacaaa tttgagatgg gcggtgaagc aggaatcgtt
     1801 gccggtcccg tgggccgcga ggcggcagct tccacaaaca cgacattgga tgcggggatc
     1861 ctgtcttact ctcgcagcaa aggagcgttc attggcgccg cactcaaagg agtcgcgatc
     1921 agtccggaca acgatctcaa ccaggctgtc tataacatga aagccaagga gatgttgatg
     1981 ggcaagccaa tgacaatcgc aacgatgccg gcaagtgtca gaattttccc gcgcacgtta
     2041 gctcgctatt ccatccgtta gtgcactcaa gctctttcat gggcgcgtcg cataatcgat
     2101 cgggaagtta cttgacggtt ctgttggcgc gccctagaat gcctcgtcac cttaaggggt
     2161 gttcctcttc aagcgggcat caggtaataa gcatcgggga atgctgcaac ttatgctccc
     2221 acgcactcaa agacaaaaag aaattctcga ctacatcacg cttttcctcg agcggcacgc
     2281 ttacgaacct tcttacgcac agatcgccag acacttcggc gtaaagtcgc gcgcaacgat
     2341 tgccaaacac gtcgctgctc ttgagcgccg cggtctgcta acgcgcaaga atgaagaagg
     2401 cgcctttgcg ttgtcagtga aagttgacga cacggatgcg ctttgcgaag tgaagttgtt
     2461 gggcagaatt gccgctggcg cgccgattga agtagtcagt gatgaagaaa cgattcccgt
     2521 gccgcgcttt cttctgggcc gcgtgcgacc tgagcgagtc ttcgctctga aagttaaagg
     2581 cgactcgatg atcgacgagc acatctgcga cggcgacata gccctgatcg agaatcgcac
     2641 tgagg
//
LOCUS       DASUEX010000061         3389 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_931494, whole genome shotgun sequence.
ACCESSION   DASUEX010000061 DASUEX010000000
VERSION     DASUEX010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3389
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_931494"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..685)
                     /locus_tag="VFV61_01070"
     CDS             complement(<1..685)
                     /locus_tag="VFV61_01070"
                     /inference="COORDINATES: protein motif:HMM:NF024785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HEX4899088.1"
                     /translation="MVSTKPARGMRDFLPADVRRRDYVIGVIKEVYKRYGFEPLETPA
                     AENIETLTGKYGEEGNQLIFKILKRGVHESTGEADLALRYDLTVPLARVVAEYRDQLP
                     RFFKRYQIQPVWRADRPARGRFREFYQCDVDFLGSRSMIVETEICAAAIEALTKLGFS
                     DFKLRLNHRQVLTGVLERAGVPLNKHDAALVAIDKLDKIEAEGVTRELTAREIEKSAA
                     DRLIDFFDGL"
     gene            complement(772..1302)
                     /locus_tag="VFV61_01075"
     CDS             complement(772..1302)
                     /locus_tag="VFV61_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEX4899089.1"
                     /translation="MIRPFGSVHPQIHSSAYVDESAQVIGDVHLGEEASIWCNATVRG
                     DMYYIRIGNRSNVQDNSVIHTRTGSHPTILEDEVTIGHSVTLHGCHIEQGSLIGIGSI
                     VLDDVRVGTQSLVAAGSLLTPGMIIPPRSLVMGAPAKVKRPLTEEEIAGLNPFWQNYV
                     EYVKKYRSQETGVRRQ"
     gene            1644..2225
                     /locus_tag="VFV61_01080"
     CDS             1644..2225
                     /locus_tag="VFV61_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899090.1"
                     /translation="MIRWKLVVTCAGVLLFAGACGGSLYKVKPVVELSPLTNEAKRGS
                     AGGLDFAVAPLLSDEESQDLFEANLPLAGVLPLRIELGYESGVPVEIKKTRFHLRDGA
                     GKEWKLVSPKQAISRILKANEVYAYNPNSRKQFEKEFGAYGLDLKTPLTAGERRRQGF
                     LFFQTPDKEAVSSPRGLVLVIEGLTEPLSISIN"
     gene            complement(2242..2679)
                     /gene="dut"
                     /locus_tag="VFV61_01085"
     CDS             complement(2242..2679)
                     /gene="dut"
                     /locus_tag="VFV61_01085"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF001862.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEX4899091.1"
                     /translation="MSDSTKTLRFQQLDSRAVLPTRGTPHSAGLDLVCIEETRLEPKT
                     RIGARTGLSVEIPEGFYGRVAPRSGLAAKQGIDVLAGVIDADYRGEVICLLYNTSDEL
                     VVLPAGSKICQLIIEQVITPRAIWANELEATARGVGGFGSTGQ"
     gene            complement(2747..3313)
                     /gene="dcd"
                     /locus_tag="VFV61_01090"
     CDS             complement(2747..3313)
                     /gene="dcd"
                     /locus_tag="VFV61_01090"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HEX4899092.1"
                     /translation="MSIKSDSWIRRMAEQHAMIEPFEPQQVKQVEGRRVVSYGTSSYG
                     YDVRCADEFKLFTNVYSAIVDPKEFDSKSFVDMKGDHVIIPPNSFALARTVEYFRIPR
                     NVMTICLGKSTYARCGIIVNVTPFEPEWEGYVTLEFSNTTPMPAKIYANEGVAQVLFF
                     EADEVCETSYKDRGGKYQGQHGVTPPKL"
BASE COUNT          729 a          957 c          912 g          791 t
ORIGIN      
        1 tcaaaccgtc aaaaaagtct atcagcctgt cagcggctga cttctcaatt tcgcgggcgg
       61 ttaattcgcg cgtcacgcct tcggcctcga tcttgtcgag tttgtcgatc gccaccaggg
      121 ccgcgtcgtg cttgttaaga gggacgccag ccctttcgag gacacccgtc aaaacttgcc
      181 ggtgattaag ccgcagcttg aagtcgctaa aaccgagttt agttaaagcc tcaatcgccg
      241 ctgcacaaat ttccgtttcg acgatcattg agcgcgagcc caggaaatca acatcgcatt
      301 gatagaactc gcgaaagcgt ccacgcgccg ggcgatctgc acgccagact ggttgaattt
      361 gatagcgctt gaaaaaccta ggtaactggt cgcggtattc agccacaacg cgcgcaagcg
      421 gcacggtgag atcgtagcgg agggccagat ccgcttcccc agtgctctca tgcacgccgc
      481 gtttcagaat cttgaagatg agttgattgc cctcttcacc gtacttccct gttagggttt
      541 cgatattctc cgccgcagga gtctccagag gttcaaaacc ataacgcttg tatacttcct
      601 taatgacgcc gatgacgtag tcgcgtcgtc gcacgtccgc aggaagaaaa tcgcgcatac
      661 cgcgcgctgg ttttgtggaa accatggtct tgggtcttgg ggtctgaggg ctttggtctt
      721 tgaactttga acttccctac tgccttcagc ctcctgacta ctgtcttatg actactgtct
      781 tctgactcct gtctcctgac tcctgtattt cttcacgtac tcaacatagt tctgccaaaa
      841 cgggttcagc ccggcaatct cttcctcggt aagcggccgc ttaactttcg cgggcgcgcc
      901 catcacgagc gagcgtggcg gtatgatcat tccgggagtc agcagggagc cggccgccac
      961 gagcgattgc gtgccgaccc ggacgtcgtc caggacaatc gaaccgatcc caatgagcga
     1021 gccttgctcg atatggcagc catgcagtgt gacgctgtgg ccgatggtaa cttcatcctc
     1081 caggatggtc ggatgcgatc cggtgcgcgt gtgaatgacg ctgttgtcct gaacattcga
     1141 gcggttgccg atgcgaatgt agtacatatc gccgcgcaca gtggcgttgc accagatgga
     1201 ggcttcttcg cccaggtgaa catcaccaat cacctgggcg ctctcatcaa cgtaagctga
     1261 gctgtgtatc tgcggatgga cagagccgaa tggtctaatc atctttttca aaatagtaac
     1321 acaacgcttt ggagtgcggt ggtcttcgca gttggatcgc ggaaggactg cctcagaacc
     1381 ctgcgcgagt taacaaacgg cttgccgccg gatgtgaggc gtggctttgc ggcgccgcac
     1441 acgcctcaca tcgatttcgg ggctctgcca cccgtgtccc tttctcgcta ggctgaactc
     1501 caggccgcag ccctgatgaa taagaagatc gcgttcggcg cggcaatgcc acgcctcaca
     1561 tccggcggca agccgaacag acataggcgc atttcgggcc acagtgtgtg cgaccgccca
     1621 aacgctatgc taaattgtgg cccatgattc gctggaaact agtcgtgact tgcgcgggag
     1681 tgcttctatt cgccggcgcc tgtgggggct cgctctataa ggtcaaaccg gtagtggagc
     1741 tgtcaccgct gaccaacgag gcgaagcgtg gcagcgccgg cggactagac ttcgctgttg
     1801 ctcctcttct ctccgatgaa gagagccagg acctcttcga agccaatttg ccgttggcgg
     1861 gcgtgctgcc cttgcgaatt gagttaggtt acgagagcgg cgtgccggtg gagatcaaga
     1921 aaactcgttt ccatttgcgc gatggcgccg gcaaggaatg gaaactcgtt agtcctaaac
     1981 aagcaatttc gcggatactg aaagctaacg aggtttacgc ttacaaccca aactcgcgaa
     2041 aacaatttga aaaagagttt ggtgcgtacg gtctggatct gaagacgccg ctcaccgccg
     2101 gcgagcgtag acgacagggg tttctgtttt ttcaaacgcc ggataaagaa gctgtatcca
     2161 gcccacgggg cctcgtgctt gtcatcgaag gtctaactga acctctgtcg atctcaatca
     2221 actgagtccg gcgctcaagt ctcactgtcc ggtcgatcca aacccaccta cgccgcgtgc
     2281 agtcgcttcg agctcattag cccatatcgc cctgggagtg atcacctgct caatgatgag
     2341 ctgacagatc ttgctgccgg ccggaagcac caccagctcg tcgcttgtgt tgtagaggag
     2401 acagatgacc tcaccgcgat agtcggcatc aatcacaccg gccagcacat caattccctg
     2461 cttcgccgcc aaccccgatc gcggtgcaac tctgccgtaa aagccttcgg gaatttcaac
     2521 gcttaagccc gtccgggcgc caatccgggt cttgggttca agccttgtct cctcgatgca
     2581 aaccaggtca aggcctgcgg aatggggtgt cccgcgcgtg ggaagcaccg cacgcgaatc
     2641 gagttgttgg aaacgcagag tctttgttga gtctgacatc gttatttctt cagcggctta
     2701 accacaaacg aatcacgaaa cctcacgaaa tgcccgtttc agcagcctac agtttcggag
     2761 gagtgacgcc gtgctggccc tgatattttc cgccgcgatc cttgtaggag gtctcgcaaa
     2821 cctcgtcggc ttcgaaaaac aaaacctggg cgacaccctc gttggcgtaa atctttgccg
     2881 gcatcggcgt ggtgttggaa aactcgagcg tcacataacc ttcccactcc ggctcaaagg
     2941 gcgtgacgtt gacgatgatg ccgcagcgcg cgtaagtgct ctttcctaaa cagatcgtca
     3001 tcacatttcg cggaatccgg aagtactcca cggttcgcgc cagggcaaaa ctatttggcg
     3061 gaatgatgac atgatcgcct ttcatgtcga caaagctttt agagtcgaac tctttaggat
     3121 cgacgatggc actgtatacg tttgtgaaga gtttgaactc gtccgcacaa cgcacgtcat
     3181 agccgtagct tgaagtgccg taagaaacca cgcgccggcc ctcgacttgt ttcacttgct
     3241 gtggttcgaa tggttcgatc atggcgtgct gctctgccat gcgacgaatc cacgaatcag
     3301 acttgatgct cataagcttc ggaggataca ggccacctct tgattgtgac aaccggactc
     3361 ttgttttctt ttttggtctt tggtctttg
//
LOCUS       DASUEX010000062         2419 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_817127, whole genome shotgun sequence.
ACCESSION   DASUEX010000062 DASUEX010000000
VERSION     DASUEX010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_817127"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..888)
                     /locus_tag="VFV61_01095"
     CDS             complement(<1..888)
                     /locus_tag="VFV61_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX4899093.1"
                     /translation="MATGLVIHISSGDDKHTEVLTGDRVRIGNCDDCDLRLRASALPK
                     RPDSDGIVLELAGKNGSYSVVGFDPSLTLSLNGQPLEMDVHIKDGDELRIADSILSLQ
                     FFPLRALPAVIPDSSRETHVAPFIEAAAIESAATARRDDAKVFLREFTRELVREINPS
                     TKLITLAIALALVGGVLYIGFSMFKEMQRSRRLIDDQRAQLTSMKDQVSKTNDTLDDL
                     GRSNKEIRDSLSLAVKLRSDYGSGVCLIAGSFYFVEAGTGRPLRYPEAQMNESGTAVQ
                     GSGDGQTLTPEGRASIAEFE"
     gene            1114..1563
                     /locus_tag="VFV61_01100"
     CDS             1114..1563
                     /locus_tag="VFV61_01100"
                     /inference="COORDINATES: protein motif:HMM:NF013426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HEX4899094.1"
                     /translation="MSIMGDTPFITDSDRLLNLAPVAAAPITHHVFVCTGTSCSAVNS
                     AEVKATFERELLARGILFGKEKKGKNPRGNIVLTECASVGFCAIGPAVMVYPDGVWYA
                     QVRTTDVAEIIEEHILNGRVVERLALLKIPSAGLNVAPTSEKQSWEA"
     gene            complement(1604..>2419)
                     /locus_tag="VFV61_01105"
     CDS             complement(1604..>2419)
                     /locus_tag="VFV61_01105"
                     /inference="COORDINATES: protein motif:HMM:NF044806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899095.1"
                     /translation="LGLFEKNIQQICTIVHEAGGLVYRDGANMNALVGIARPGDMGVD
                     VIHLNLHKTFSTPHGGGGPGCGPCCCTKELEPFLPVPRIVKDQAVYKLDSNYPRSIGR
                     VKAFLGNYGMMLRALAYIQTHGHDGLREATEAAVLNSRYVAHGLATDYERPFAQPSMH
                     EVVFTDKRQSRKGVHTLDIAKRLIDYGFHPMTIYFPLIVQGAMLIEPTESVGRAELDQ
                     FVEAMRSIAREAVEDPDLVINAPHSTRIGRLDEAAAARRPVLRWKPTQAASAG"
BASE COUNT          527 a          650 c          690 g          552 t
ORIGIN      
        1 ctcaaactct gctatggagg cccggccctc cggggtcaag gtctgaccat cgccactacc
       61 ctggaccgca gtcccgctct cattcatctg cgcttcagga tagcgcaagg gccggcccgt
      121 tccagcttcg acgaagtaga acgaaccggc aatcaaacag accccactgc cataatcgct
      181 ccgcagtttt acagcgagcg aaagactgtc ccgaatctct ttgttcgaac gccccaagtc
      241 gtcgagtgtg tcgttggttt tcgagacctg gtccttcatc gaggtgagtt gcgcgcgttg
      301 gtcgtcaatc aggcgccggc tgcgctgcat ctctttgaac attgaaaagc cgatgtagag
      361 cacgccgcca acgagagcga gagcaattgc cagcgtgatt agtttggtgc tgggattgat
      421 ttcacgaaca agctcgcggg tgaattcccg caagaacacc ttcgcgtcat cgcggcgggc
      481 ggtggctgca gactcgatcg ctgcggcttc gatgaagggt gcgacgtgtg tttcccgaga
      541 cgaatcagga ataacggcgg gcaaggcacg caggggaaag aattgcaggg aaaggatcga
      601 atcggctatg cgcagctcgt cgccgtcttt gatatggaca tccatctcga gcggttgccc
      661 gttcagactg agagttaagc tcggatcaaa tccgaccacg ctgtaggagc cattttttcc
      721 tgcaagctcc aacacgatgc cgtcactatc cggacgtttt ggcaacgcgg aagctcgcaa
      781 acggagatca caatcgtcgc aattgccaat gcgaacgcgg tcgccggtta acacttcagt
      841 gtgtttgtcg tcgcctgagg agatgtgaat aacgaggccg gttgccataa aactgcggat
      901 tcgtgcgccg ccctaatagt aaggtgggcg cccgcttcag accgctagtt cctggttgcg
      961 gtctactgag cggtcgccct tctagcgttc attctacaat gtattttcaa agtgtagcaa
     1021 agtaagtttc tgaaattgtc gagtggtgat tggcgattgc caattgtcct ccctgccgac
     1081 cgggcacgat cgtgttagca tgaggctttg cggatgagca ttatgggcga tacacctttc
     1141 atcaccgatt ccgaccgcct tctcaacctt gcgcctgttg cggcggcgcc gattactcat
     1201 catgtatttg tgtgcacggg aacgtcgtgt tcggcggtga acagcgccga agtaaaggcg
     1261 acctttgaac gggaactact ggctcgggga attctctttg gtaaagaaaa gaagggaaag
     1321 aatccgcggg gaaatattgt cttgaccgag tgtgcttccg ttggtttctg cgccatcggg
     1381 ccggcggtaa tggtttatcc ggatggtgtt tggtatgcgc aggtgcgcac gaccgacgtg
     1441 gcagagatca tcgaggagca catcctgaat ggaagggtag ttgagagact ggccttacta
     1501 aagattcctt cggcgggcct gaacgtcgct ccaacttccg agaagcaaag ttgggaagca
     1561 tgaatgtagg gacgccccta caaataagcc ggacgcgtgg tcactatccc gcacttgctg
     1621 cttgagtcgg cttccaacga agcacaggtc gccgcgccgc agccgcttca tcaagtctcc
     1681 caattcgggt tgaatgcggc gcattgatca ccaggtccgg atcttcgaca gcctctcgcg
     1741 cgatcgacct catcgcctcg acaaactgat ccagttcggc tcgacccact gattcggtcg
     1801 gctcaatcag cattgctccc tgcacaatta acggaaagta gatcgtcatt ggatgaaagc
     1861 cgtagtcgat caatcgcttg gcgatgtcga gcgtgtgcac tccctttcgc gactggcgtt
     1921 tatctgtgaa gacaacttca tgcatcgagg gctgtgcaaa cggccgttcg taatcagtgg
     1981 ccaggccatg tgcgacataa cgtgaattca agactgccgc ttccgtagct tcccgcaatc
     2041 cgtcatggcc gtgagtttga atgtaggcga gcgcacgcag catcatgccg tagttgccga
     2101 ggaaagcctt cacccgacct atcgatcgcg ggtagttaga gtctaatttg taaacagcct
     2161 ggtctttaac aatgcgcggc acaggtaaga agggctcaag ctctttggtg cagcaacaag
     2221 gtccgcagcc gggaccgccg cctccgtgcg gagtggaaaa ggttttgtga agattcaggt
     2281 gaattacatc gacgcccatg tcgccggggc gggctatgcc gaccagtgcg ttcatgttgg
     2341 cgccgtccct gtagaccagg ccaccggcct catgaacgat cgtgcagatt tgctggatgt
     2401 tcttttcgaa tagtcccaa
//
LOCUS       DASUEX010000063         2138 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1046348, whole genome shotgun sequence.
ACCESSION   DASUEX010000063 DASUEX010000000
VERSION     DASUEX010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1046348"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1856
                     /locus_tag="VFV61_01110"
     CDS             <1..1856
                     /locus_tag="VFV61_01110"
                     /inference="COORDINATES: protein motif:HMM:NF014869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HEX4899096.1"
                     /translation="PGGTEGVVMTTKSSKKPAKKGSESTKSSTSKRTTKTKLKKSEAA
                     SAAPDSFRKSDVGRNGLRSFAFHRQDKGANTVLGAIRPQVRRGPSGARPTAAAARASV
                     TGMDAETTARLYLTNALASDQLPTFTAGDANAEKSEFKLIAVEAVPLTGTQMVKFCQY
                     YRRIPVYGSLVAVELDKKNELISINSTLTEPTNVDAVASVAPADVLKTVARASGADAQ
                     GLEATPSVYYYFDREAQRWRLVYIMRDVPKTKGGDDIEVVTEGVPNRRQYSAPRAFDY
                     VIDAHSGDLVAELPRSHDASIKALDGLKKSRTIGFSQNANGSFLHDPDLNLHTFDFGF
                     GNVDALVSKLPGTYVSNPPDPWDGAAVSAHANAADVLRFLSSVLKRNGVDNIGGPVRS
                     SINCVEEDGTKEWDNATWYRKQMMYGQSKKDGKLRSWALAKDIVAHELTHGITDYTAR
                     IEYLGESGALNESYSDIFGVIVANIGQPDVKKWNWLIGEGLMDGEIALRDMRRPERFD
                     QPSHMDDYQKLPKTEAGDWGGVHTNSGIHNKAAYNMLTAKDAQGKYLLKPREVAALFY
                     LALTQNLSRTSRFGDSRRGVELAARSLFANKTNKQARLKAVAKAFDKVGIV"
BASE COUNT          517 a          576 c          597 g          448 t
ORIGIN      
        1 tacctggcgg aaccgaagga gtagttatga ccacgaaatc gtcgaagaaa ccggcaaaga
       61 agggctcaga atccactaaa tcatcaacaa gcaaacgaac tacgaaaaca aaattgaaga
      121 agagcgaagc ggccagcgcg gctcccgatt cttttcgtaa gagcgacgtc ggtagaaatg
      181 gcctgcgctc ctttgctttt caccggcaag acaaaggtgc gaacacggtg ctgggcgcca
      241 ttcgtcccca ggtgcgaagg ggaccgtctg gcgcgcgacc cactgcagca gctgcgcggg
      301 ccagtgtcac cggaatggac gccgagacca cagctcgact ttatttgact aacgcactgg
      361 caagcgacca actcccgact ttcaccgctg gcgatgccaa tgccgaaaag agtgaattca
      421 aattgatcgc ggtagaagca gtgccgctca cgggcacgca gatggtgaag ttctgccagt
      481 actaccggcg cataccggtt tacggttcgc tggtggcggt ggagttagac aagaagaacg
      541 aattgatttc tatcaactcc acacttactg aaccgacgaa tgtcgatgcc gtggcgagcg
      601 tggctccggc tgatgtcttg aagacagtag ccagagcgtc aggcgccgat gcacagggtc
      661 tggaggctac accgagtgtc tattactact tcgaccgtga ggcgcaacgt tggcgtcttg
      721 tctacatcat gcgtgacgtt cctaagacaa agggtggaga tgacattgaa gtcgtgacag
      781 aaggcgtacc gaaccggaga caatacagcg cgccgcgtgc cttcgactat gtcattgatg
      841 ctcactcggg cgacctggta gctgagttgc cgcgttccca cgatgcttcc ataaaagcgc
      901 ttgacggact gaagaagtcg cgcactatcg gcttttcgca aaacgccaac ggctcttttc
      961 ttcacgaccc ggatctgaat ctgcacacct tcgactttgg ctttggcaat gtcgatgctc
     1021 tcgtcagcaa gcttccggga acgtacgtct cgaatccgcc agacccctgg gatggagccg
     1081 ctgttagcgc tcatgccaac gctgcagatg tgttgagatt cctcagttcg gttctgaaac
     1141 ggaatggagt ggacaacatc ggcggcccgg ttcggtcgag catcaattgc gtggaagagg
     1201 acggaaccaa agagtgggac aacgcaacct ggtatcgcaa gcagatgatg tatggacaga
     1261 gcaaaaagga cggcaagctg cgttcatggg cacttgccaa ggatattgtt gctcacgaac
     1321 tgacccatgg aataaccgac tacactgctc ggatagagta tctcggagag tcaggcgcgc
     1381 tgaatgaatc ctactctgac atctttggag tgatcgtcgc gaacattggt cagccggacg
     1441 tgaaaaagtg gaactggctt atcggcgaag gcttgatgga tggcgaaatc gccctgcgcg
     1501 atatgcgccg tccggaacga ttcgaccagc cttctcacat ggatgattac cagaagcttc
     1561 ccaagacgga agccggcgac tggggtggcg tgcataccaa cagcggcatt cacaacaagg
     1621 ctgcttacaa catgcttact gcaaaggatg cgcagggtaa gtacttgttg aagcctcgtg
     1681 aagtggctgc cctgttctat ttggctctaa cacaaaacct ctcacgcacg tcgcgttttg
     1741 gcgacagccg gcgcggcgtc gaactcgcag cgcggagctt attcgcaaac aaaaccaaca
     1801 agcaggcgcg tcttaaggcc gtggcgaagg cgtttgataa agtagggatc gtttagctta
     1861 gtggttcttc caggattgac ctgtgcacga agggccacgg gctctccgtc ctcgcgtaat
     1921 cgtaaattga ttgccaacgc ggtggccgat ccgtcaccgc gttgcaaaca cactccctcg
     1981 ctcaactcag gaggccccat gttcaaacta gtgcttgcac tgtccctctt cttgttcttt
     2041 ttagcgattt tctcacccgc gggaactggc atcgttgcga gcaaaccggc actgccgtcc
     2101 tttaactttc ccagtgcgcg agataatccg ccgccagg
//
LOCUS       DASUEX010000064         3080 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_15503, whole genome shotgun sequence.
ACCESSION   DASUEX010000064 DASUEX010000000
VERSION     DASUEX010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3080
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15503"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1994
                     /gene="tkt"
                     /locus_tag="VFV61_01115"
     CDS             <1..1994
                     /gene="tkt"
                     /locus_tag="VFV61_01115"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEX4899097.1"
                     /translation="DQLCINTIRTLSLDAVQKAESGHPGLPLGAAPMAYVLWTRFLRH
                     NPQNPKWENRDRFLLSAGHGSMLIYSLLHLTGYDLPLAEIKNFRQWGSKTPGHPENTL
                     TPGVEITTGPLGQGFANGVGMAMASAHLAAKFNQQDFPVIDHYVYAIVSDGDLMEGVA
                     SEAASLAGHLKLGKLIYFYDDNRVTIEGFTDLAFTEDVPKRFEAYGWHTVTVEDGNDL
                     EAIDAAIREAQSVGTKPSLISVKTTIGFGMPSAGTRKAHSDAPGEEAVRETKRHLGWP
                     EDQQFYIPDEALAHFRQSVAQGEKQESEWNELVSRYQAKHPELSKLWRTTMSGELPQD
                     WEQHLPTFADSKAMATRAASGEVINALAPHLPVLIGGSADLGPSNNTDIKGGGDFEAG
                     TFAGRTIHFGVREHAMGSTLTGMSLNGGLIPYGGTFMTFSDYMRPAIRLAALSEVQVI
                     YIFTHDSVGLGEDGPTHQPIEHLAALRAIPHLYVIRPADSHEVREAWRTAILRRHAPT
                     ALALTRQKVALIDRTRFGSAEGARKGAYILAEAEAANDKPSLILIATGSEVSLALEAR
                     ETLQQEGVPTRVVSMPCWELFEEQSEAYRAEVFPPSIIARLAIEAGVRQGWDRYVGPA
                     GDVICLDRFGASAPGDVALKNLGFNVDNVVRRARALLQP"
     gene            2116..2565
                     /gene="rpiB"
                     /locus_tag="VFV61_01120"
     CDS             2116..2565
                     /gene="rpiB"
                     /locus_tag="VFV61_01120"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HEX4899098.1"
                     /translation="MGTRIAIGADHAGFPLNERVIAELRTAGHEIVDFGTHDGSQVDD
                     YPDYARQVGQAIQQGAVEIGILICGSGVGAAVAANKLRGIRAAMCVDTYSAHQSREHD
                     DCNVLCLGARVVGVELALELVRTFVAARFSGEERHRRRLRKIAEMER"
     gene            complement(2719..>3080)
                     /locus_tag="VFV61_01125"
     CDS             complement(2719..>3080)
                     /locus_tag="VFV61_01125"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899099.1"
                     /translation="GQAVRDSPMNLVAVANGLYAGGGMMLSPEARNDDGVLDVVTASG
                     LNRVAVMRELPRIHSGGHVANPKVRVFQGRHVRIETFAPDDALLIEADGNVRGKTPVE
                     FRVMPLALKVVSRNLRN"
BASE COUNT          659 a          856 c          874 g          691 t
ORIGIN      
        1 ttgatcaact ttgcatcaac acgattcgga cactctccct cgatgccgtg caaaaggcgg
       61 aatcaggaca cccgggattg ccgttgggcg cagcgccgat ggcctacgtt ttgtggacgc
      121 gctttcttcg tcacaatccg cagaacccga aatgggaaaa tcgcgatcgc tttttgcttt
      181 ccgccggcca tggttcgatg ttgatttatt ccctacttca tctcaccgga tatgaccttc
      241 cgctcgctga aatcaagaac ttccgccagt ggggttcaaa gactccagga catccggaaa
      301 acactctgac tcctggtgtg gaaataacca ccggaccact ggggcaaggt ttcgccaacg
      361 gcgttgggat ggcgatggct tcggcccatc tggcggctaa gtttaatcag caggactttc
      421 cggtcatcga ccactacgtt tacgcgatcg tctcggatgg ggacttgatg gagggcgtcg
      481 cctcggaagc ggcatcgctt gccggtcatc taaaactcgg caaactaatc tacttctatg
      541 atgacaaccg cgtaacgata gaaggcttca ctgacttggc atttactgaa gatgtaccca
      601 aacgcttcga ggcttatggt tggcatacgg tgacagtgga agatggcaac gatctggaag
      661 cgatcgacgc ggcgattcgc gaggcgcaat cggtcggcac gaaaccgtct ttgatatcag
      721 taaagaccac gattgggttt ggcatgccca gcgcgggcac gcgcaaggca cacagcgatg
      781 caccgggcga ggaagccgtg cgcgaaacca aaagacatct cggctggccg gaagatcagc
      841 agttctacat tcccgatgaa gcactcgcgc actttcgcca gagtgttgct cagggagaga
      901 aacaggagag cgagtggaac gaattagtct ctcggtatca ggcgaaacat cccgagctca
      961 gcaaactttg gcgcacaact atgtcgggcg agctcccgca agactgggaa caacacttgc
     1021 caacttttgc agactcgaag gcaatggcca cacgcgcggc gagtggcgaa gtcattaatg
     1081 ctctcgcccc gcatctgccg gtgttaattg gcggctctgc agaccttggt ccgtcaaaca
     1141 atacggacat caaaggtggt ggggattttg aggccggcac ctttgctgga cgaacgatcc
     1201 acttcggagt gcgcgagcat gccatgggtt cgacgctaac gggtatgtcg ctcaacggcg
     1261 gtctaattcc ctatggcggc acgttcatga cgttctcgga ttacatgcgc ccggcgattc
     1321 gtctggcggc gctttccgaa gtccaggtga tctacatctt cactcatgat tcggtcgggc
     1381 tcggtgaaga cggccccacg catcaaccca tcgagcatct cgccgccctg cgggccattc
     1441 cccatttgta cgtcatccgg ccggctgatt ctcatgaggt gcgtgaggcc tggcgcacag
     1501 cgattttgcg acgacatgct cccacggctc tggcgttaac gcgccagaag gttgcattaa
     1561 tagaccggac ccgtttcggc tcggcggaag gcgcgcgcaa gggtgcttac attctcgccg
     1621 aagcggaagc tgctaacgac aaaccaagtc tgattcttat cgccacaggt tctgaagttt
     1681 cactggcgtt ggaagcgcgc gagaccttgc agcaggaagg cgtgcctacc cgcgtcgtct
     1741 cgatgccctg ctgggagttg tttgaagaac agagtgaagc gtaccgcgcc gaagtattcc
     1801 ctccttccat tattgcgcgt ctcgcaatcg aggcgggagt gcgccagggt tgggatcgtt
     1861 atgtgggtcc tgctggcgat gtaatttgtc tggatcggtt cggcgcttcc gcccctggcg
     1921 atgtggcact taaaaatctc ggcttcaacg tagacaacgt tgtgcggcgg gctcgggcgc
     1981 ttttgcaacc ctgacaccgt tccgacaatc gcgaagagta cggttcaaaa aagttcaagc
     2041 ccatcatgtc taatttgatc cggtcggtat cgctcccggt tctgattttc tcgacccccg
     2101 ttgcagtgca ttgtcatggg aacacgaatc gccattggtg ctgatcacgc aggcttcccg
     2161 cttaacgaac gagtgatcgc cgagctgcgg acagccggtc acgaaatcgt tgacttcgga
     2221 acccatgatg gttcgcaggt ggatgattat cccgattacg ccaggcaagt agggcaggcg
     2281 atccaacagg gtgctgtcga gattggaatc ctcatttgtg gcagcggtgt aggcgccgcg
     2341 gtggcggcta acaaacttcg cgggatccgc gcggcaatgt gtgtagacac ctattccgcg
     2401 caccaatcgc gagaacacga tgactgcaat gtgctctgcc tgggagcgcg tgtggttggc
     2461 gtcgagttgg cgctggaact ggtgcggacg tttgtggcgg cgcgttttag cggcgaggaa
     2521 cgacaccggc gccgtttgcg caagattgcg gaaatggaaa ggtaagtatg gaaaccgcta
     2581 atgagaataa agcgagcttg gtactttgtt cttagtactt tgtgcttgtt gctcagccat
     2641 aattctgcat ctcaagtttc atccgcgaac aaagttcaaa gaaccaagtt caaagctccg
     2701 tttttctccc cggcggtttc agtttcgcag gttacggctg accaccttca gtgccagggg
     2761 cataacgcga aactcgaccg gggtctttcc ccgaacattt ccgtcagctt cgattagcaa
     2821 agcatcgtcg ggcgcgaacg tttcaattct cacgtgccgt ccctgaaaaa cccgcacctt
     2881 cggattggca acgtgtcctc cgctatgaat ccgtggcagt tcgcgcatca ccgcgacgcg
     2941 attcaagccg ctggcggtca ccacatccag tactccatcg tcgttccgtg cttccggtga
     3001 aagcatcatt cccccgcctg cgtaaagacc gtttgcgact gcaaccaagt tcatcggcga
     3061 gtcccgcacc gcttggccgt
//
LOCUS       DASUEX010000065         3180 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_932252, whole genome shotgun sequence.
ACCESSION   DASUEX010000065 DASUEX010000000
VERSION     DASUEX010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3180
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_932252"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            19..>3180
                     /locus_tag="VFV61_01130"
     CDS             19..>3180
                     /locus_tag="VFV61_01130"
                     /inference="COORDINATES: protein motif:HMM:NF024644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-variant-translocated molybdopterin
                     oxidoreductase"
                     /protein_id="HEX4899100.1"
                     /translation="MSTDNRHINFALMRQYILGHQDGNRADGVAAQGKQYWRSLEELA
                     DSPALSEFVHREFPQQAEEWDDPIQRRTFLKLMGASLALAGLSGCVYQPPEKVVPYVK
                     QPEEIVPGKALFFATAFTLGGVATPVLVRSNEGRPTKIEGNPDHPNNRNGEKDTGSSA
                     TDIFSQASILTLYDPDRSQTTLYRDETRPWTTFVGEIRTLLDEQRGKQGSGMRFLTET
                     VTSPTVAAQIKAIQTEFPQAKWHQYEPANDDNARAGAVATFGQPVHTIFNFAAADRIL
                     SLDSDFLSCGPGALRYAKDFAQKRKGGDHGEGMNRLYVIESTPTNTGASADHCWTVKP
                     SEVEGLARALAQKLGANAASTGNGSSSSAAYINAVARDLQAHQGASIVIAGREASASV
                     HALAHAMNTALGNVGKTVFYTDPLAANSVDQRQSLQELINDIDGGRVELLVIVGGNPV
                     YNTPADLKLNQERMFKVKTRVHLSLYRDETSELCHWHVPESHYLETWSDTRTYDGTVT
                     IVQPLIEPLYQSRSVHEFLAVFTQQYDQNPYDIIRAYWMAHRSQPATITQAAASPTPA
                     RVAPASQSPSPAAQTQTATAAGDFENSWRKWLHDGFIPNTALPVKTLTATASGTATPA
                     APLTTGSTAGGFEIVFRTDPTIFDGRFANNGWLQELPKPLSKITWDNAAMVSPNTAKQ
                     LGLNRKMGEKGGDFYVDTLKIDYQGRTITNTIPAWITPGQPDNVITIYLGYGRRLSGR
                     VGNQHGFNAYEIRTADSPWFATGVQVQRGTAQYQLAATQTHFSMEGRDIYRSQDVGGE
                     AHEGGHGHEDPAPDETLYDPSLYDYKNQGMGYAWGMAIDLNNCVGCNGCTIACQSENN
                     IPVVGKQEVARSREMHWIRVDTYFEGADASHPESTNFMPVPCMHCENAPCEPVCPVHA
                     TVHSAEGLNDMVYNRCVGTKYCSNNCPYKVRRFNFFLYQDWDTPTYQLMRNPDVSVRS
                     RGVMEKCTYCVQRIQEAKIHSELEGRKVYDGEIVTACQAACPTEAIVFGDINDPNSRV
                     SKLKADKRNYSLLTELNTRPRTTY"
BASE COUNT          781 a          881 c          863 g          655 t
ORIGIN      
        1 gacccggatc gctttctaat gtctacggat aacagacaca tcaattttgc gctcatgcgc
       61 cagtacatcc tgggtcacca ggatggcaac agggctgatg gcgttgcagc gcagggcaaa
      121 cagtactggc gcagtctaga agagctggcc gactcgccgg cactgagtga attcgtccat
      181 cgtgagtttc cacagcaagc agaagaatgg gacgatccca ttcagcgccg cacttttttg
      241 aagttgatgg gggcatcttt ggcgctcgcg ggattgagcg gctgcgtcta tcaaccacca
      301 gagaaagtcg ttccttacgt aaaacaacct gaagaaattg ttcccggcaa ggctttgttc
      361 tttgctacgg ccttcacact cggcggtgtc gcgacacctg ttttggtgcg cagcaatgag
      421 ggaaggccaa ctaagatcga aggcaatccg gatcacccga acaaccggaa cggagaaaag
      481 gacacaggtt ccagcgccac agacatcttt tcgcaggctt ccatcctgac tctctacgat
      541 cctgatcgtt cacagaccac actttaccgg gatgaaacaa ggccctggac tacgttcgtc
      601 ggcgagattc gcaccctcct tgatgagcaa cggggaaaac aaggtagcgg catgcgcttc
      661 cttactgaaa ccgtgacttc ccccactgtc gccgcccaga tcaaggcaat acagaccgaa
      721 ttcccgcaag cgaaatggca ccaatacgaa cccgcgaatg atgacaacgc tcgcgccggg
      781 gccgttgcga cgtttggtca accagtacac acgatcttca acttcgctgc tgccgatcga
      841 atcctttcac tggattcgga tttcctttcc tgtggtccgg gtgcactgcg ctacgccaaa
      901 gattttgcgc aaaagcgtaa aggtggcgac cacggcgaag ggatgaatcg gctttatgtg
      961 attgagagca caccgacaaa cacgggtgcg agcgccgatc attgctggac cgtaaagccg
     1021 agtgaagttg aaggtttggc gcgcgccctc gcgcaaaaat tgggggcgaa tgctgcatcc
     1081 accggcaatg gatcctcgtc tagcgccgca tacatcaatg ccgttgcgcg agacctgcaa
     1141 gcgcaccagg gcgcaagcat tgtcatcgcg ggccgagagg cgtccgcctc cgtgcatgcc
     1201 ctggcgcacg cgatgaatac cgcactaggc aacgtcggaa agaccgtttt ttacactgat
     1261 ccgttggcgg caaattcagt cgatcagcgc cagtcgttgc aggaactaat caatgacatc
     1321 gatggtggac gcgtcgaatt gctggttatt gtgggtggga acccggtcta caacacacct
     1381 gctgatttga agttgaacca ggagcggatg ttcaaggtaa agacgagggt acatctcagt
     1441 ctctaccgcg atgagacttc cgagctttgt cattggcacg tgccggagag tcactacctt
     1501 gagacgtgga gcgatacacg cacctacgac ggaaccgtaa caattgtcca gccgttgatt
     1561 gagccgcttt accaaagcag aagcgtgcac gagtttctgg cggtcttcac ccaacagtac
     1621 gaccagaacc cttatgacat catccgggct tattggatgg cacacagaag tcagccagcg
     1681 acaatcacgc aggctgcggc cagtccaact ccggctcgcg tagcgccagc cagtcaatca
     1741 ccgagcccgg ctgctcaaac tcaaaccgca actgctgcag gcgactttga aaactcatgg
     1801 cgcaagtggt tgcacgatgg gttcattcca aacactgcct tgcccgtcaa gactcttaca
     1861 gctactgcga gtggcaccgc aacacctgcg gctccgttaa caaccggatc aacagcgggt
     1921 ggttttgaga tcgtcttccg aaccgatccg acgatcttcg acggacgctt tgcgaacaat
     1981 gggtggttgc aggagctgcc taagccactt tccaagatca cctgggacaa cgctgcgatg
     2041 gttagtccaa ataccgcaaa gcagttgggt ctcaacagaa aaatgggtga gaagggcggc
     2101 gacttttacg tcgacacgct aaagatcgat taccagggtc gcactatcac aaacacgatt
     2161 cctgcgtgga ttacacctgg tcaacccgac aacgtgatca ccatctatct cggctacggc
     2221 cgcagacttt cgggacgtgt cggcaaccag catggcttca acgcttatga gattcgaaca
     2281 gccgattccc cctggtttgc aaccggcgtt caggtgcaac gtggcacggc ccaatatcaa
     2341 ctggcggcaa ctcaaaccca cttttcgatg gaagggcgtg acatctatcg ctcgcaagat
     2401 gtgggcggag aagcgcatga ggggggtcac ggtcatgaag atccagcgcc agacgaaacg
     2461 ctctacgatc cctcgctcta cgactacaag aatcaaggaa tgggctatgc gtggggaatg
     2521 gcgatcgatc tgaataactg tgtgggctgt aacggctgca ctatcgcctg ccaatccgaa
     2581 aacaacattc ccgttgtagg gaagcaggag gtggcacgga gccgcgagat gcattggatt
     2641 cgcgtcgata catatttcga aggcgcggat gcgagccatc ctgagagcac taacttcatg
     2701 cctgtaccct gcatgcactg cgagaacgct ccgtgcgaac ctgtctgccc ggtccatgcc
     2761 accgtgcaca gtgcagaagg tttgaacgac atggtctaca accgttgcgt ggggaccaag
     2821 tactgttcca acaactgccc atacaaagtg cgtcgtttca acttcttcct gtatcaggat
     2881 tgggacaccc caacttatca actgatgcgg aatcctgacg tttcagtgcg cagccgcggt
     2941 gtgatggaga aatgcaccta ctgtgttcag cgcatccagg aagcaaagat tcattcggag
     3001 ttggaagggc gaaaggtcta tgacggcgag attgtgactg cctgtcaggc agcttgcccg
     3061 accgaagcga tagtttttgg agacatcaat gatccgaaca gccgcgtttc taaattgaaa
     3121 gctgacaagc gcaactattc gctgctgacg gagctgaata cgcggccgcg cacgacctat
//
LOCUS       DASUEX010000066         3551 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_474753, whole genome shotgun sequence.
ACCESSION   DASUEX010000066 DASUEX010000000
VERSION     DASUEX010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3551
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474753"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1053
                     /locus_tag="VFV61_01135"
     CDS             <1..1053
                     /locus_tag="VFV61_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899101.1"
                     /translation="FMTDGLPTAGETNPTRILDNAVQSRIPGVRLFSFGAGYHVNTAL
                     LDKLAADNGGVADYIEPNEDLEAKVSTFFARVNSPVLTDLRLDMAGVETDLVYPRALP
                     DVFRGSQVMLIGRYRNDVAMDYVRLQLSGKSGGSSKEYVYNNQRFPLREEANDFLPRL
                     WATRRVGWLMEQIRTNGEAAELRDEVVDLGTRYGIVTPYTSYLALEPGAGPQTITRLG
                     GAVGVGRNNNFKIDAAAPPAPAKAAEARVMTGTLGVRESKRARSQQDAERFDKGGLSA
                     AVRTVGGKTFYLREGVWTDAEFKAEAKLPETSLKFGSDEYFTLLTRKPKLGDFFALGE
                     RVIVVFDGRVYRVTGP"
     gene            1274..2260
                     /locus_tag="VFV61_01140"
     CDS             1274..2260
                     /locus_tag="VFV61_01140"
                     /inference="COORDINATES: protein motif:HMM:NF026220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HEX4899102.1"
                     /translation="MKFGNKQKSGRYLATAIVLAATSAVALAQQGWVATRIAPPKQEL
                     NTVFFLDSKRGWAGGDDGFLIRTEDGGTTWTNQRVETTAAINDIYFRDKERGFLVAGN
                     AIFTTIDNGIRWSEARRFAPAEFEGATVELYCIRFSSKKKGWVVGSVSKRDRVIDSIL
                     VYTDNAGETWQRKRTPSNSELIHVDFDNDKRGWIVGEGGTILFTNDNGDTWTRQNSGT
                     DVTLYHVDFRNDKKGLAVGAKGTLLRTFDGGDNWMIVRTSVPATLLSVEFVNEDEAWA
                     VGRGGTILRSGDGGVSWLKQDSNITHNLYALQFQKKIGWAVGSNGMILRYER"
     gene            complement(2430..>3551)
                     /locus_tag="VFV61_01145"
     CDS             complement(2430..>3551)
                     /locus_tag="VFV61_01145"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX4899103.1"
                     /translation="ADLVAKMGATLSAGANSDYTTVAASSLTVFHDRILGLLADVALN
                     PSFPENEVKLIRENTKESLRQQRAQPSFLATEMVSRIIFGEHPYSKIAPTIDSLEATT
                     REKLVEFHRSQFVPNNAVLVVAGDVDPDALIQRAENLFADWKPGAVQADKFPEPPPRS
                     SRSAYLVDRPGSAQSNIVIANGGGITRTSPDYFPMLLMHTVLGANASSRLFMNLREDK
                     GYTYGAYSSLDARRSAGSFRASAEVRTPVTGDSLKEFFYELDRIRSEPVSEKELEDAK
                     SYLTGVFPIRLETQEGLIEQIVQIKMLGLPDDYLRTYRQRIQEVSIPELQRVANEYIR
                     PDEAAIVIVGDGEEISQQVKPFAETVETYNTAGQRTGNK"
BASE COUNT          842 a          900 c         1015 g          794 t
ORIGIN      
        1 ttcatgaccg acggtctgcc gacggctggt gaaacaaatc cgacgcgcat tctcgacaac
       61 gctgtccaat ctcgtattcc cggcgtgcga ctctttagtt tcggtgcggg ctaccacgtc
      121 aacacagccc ttttggacaa actggcagcc gataatggtg gcgtggccga ctatattgaa
      181 ccgaatgagg atctcgaagc gaaagtttcg actttctttg cgcgcgtgaa ctcccccgtg
      241 ttgactgact tgcgcctgga tatggcagga gttgaaacgg acctggttta tccgcgagct
      301 ttaccagatg tcttcagagg ctcgcaggtg atgctaatcg ggcgttaccg gaatgacgtc
      361 gctatggatt atgtgcgttt gcagttgagc ggaaagtccg gcggcagcag caaagagtac
      421 gtttacaaca accaacggtt cccactacgc gaagaagcca acgattttct gccgcggttg
      481 tgggccacta gacgagtcgg ttggttgatg gaacagattc gtactaacgg cgaagcagca
      541 gagctgcgcg atgaggttgt ggatctgggg acacgttatg gaattgtgac gccttataca
      601 tcgtatctgg ccctggagcc gggtgctggt ccgcagacaa taacgcggct tggtggagct
      661 gtgggagttg gcaggaacaa caatttcaag atcgacgccg cggcaccgcc ggcacctgct
      721 aaggctgcag aggctcgcgt gatgaccggg acacttggcg tgcgggaaag caagcgagca
      781 cgctcgcagc aggatgctga gcgcttcgac aaaggcggat tgtccgcagc cgtgcggact
      841 gtcggtggca agaccttcta tctgcgcgag ggagtctgga cagacgcgga attcaaggct
      901 gaggcgaagc ttccggaaac ttcgctcaag ttcggcagcg atgagtactt cactctgcta
      961 accaggaaac cgaagttagg ggatttcttt gccctcggcg aaagggtgat cgtggtcttc
     1021 gatggccggg tttatcgagt gacagggcca taacaaggtt gtcgcagaaa cattgaaata
     1081 taaaaccagg gccacgcgtt catcgggttg aatgcgcggc ccatttctgt tttggtcgga
     1141 atcttgtctt gtgaacccga actaaggtac aaagaagcga ccaggaacaa tcgagcaacg
     1201 cgcaggcgct gagagagctc gcttgtcttt cagtgtctca aggtgacaag ggattcgtag
     1261 tccgggagag caaatgaagt tcggaaacaa gcaaaaatca gggcgctatt tggcgacggc
     1321 aattgtctta gccgccactt ccgcagtagc gcttgcgcaa caagggtggg tggccactcg
     1381 catcgcgccg ccgaaacaag aactgaatac tgtctttttt ctcgacagca aacgtggctg
     1441 ggctggtggt gacgacgggt ttctgatccg cactgaggac ggaggcacca cctggacgaa
     1501 ccagcgcgtc gaaaccaccg cggcaatcaa cgacatctat tttcgggaca aagagcgcgg
     1561 attcctggtc gccggtaatg ccatcttcac gacaatagat aacggcatcc gctggtcaga
     1621 agcgcgccgt tttgcacccg cagaattcga gggcgccact gtagagctgt actgcatccg
     1681 tttttcgtcc aagaaaaaag gttgggtagt gggctcagtc agcaaacgcg atcgagtaat
     1741 cgacagtatt ctggtttata cagacaatgc cggagaaaca tggcagcgca aacgcacccc
     1801 gagtaactcc gagctgatcc acgttgattt cgataacgac aagcgtggtt ggattgtcgg
     1861 cgagggaggc accatcctct tcacaaacga taatggcgat acctggacgc ggcagaattc
     1921 aggaaccgac gtcactctgt atcatgtgga ctttcgcaat gacaagaaag ggctcgcggt
     1981 aggcgcgaaa ggaacgttat tgcggacatt tgatggcggc gataattgga tgattgtgag
     2041 aacaagtgtt cccgccacac tcttgagcgt tgagtttgtt aatgaagacg aggcatgggc
     2101 agttggtcgt ggcggaacca ttctgcgaag tggcgacggc ggcgtttcct ggctcaagca
     2161 ggattctaac attactcata atttatatgc gcttcagttt cagaagaaga ttggctgggc
     2221 agtgggcagc aacggcatga tcctacgcta cgagcggtaa gtctccaaaa cgcaccccaa
     2281 gcataaaaaa ccgcccggca cgttggccgt gccgggcgaa atcaggagga accgagtgtt
     2341 acggtcgtcg ctatcaatct gcgacgatcc attaacctcg acgcttaaga tgagattcag
     2401 acgggatgcg ttgctctaat gctcattctt catttattac ccgttcgctg gcccgctgta
     2461 ttgtaagttt caactgtttc cgcaaatggt ttcacttgct ggctaatctc ttcgccgtcc
     2521 ccgacgataa cgatcgcggc ttcatccggg cggatgtatt cattagccac gcgttgcaac
     2581 tcaggaatgc tcacttcctg aattcgttgc cggtaggttc gcaaatagtc gtccggtaag
     2641 ccgagcatct tgatctggac gatctgctca atcagtcctt cctgcgtctc caggcgaata
     2701 ggaaatacgc ccgtcaaata cgacttagca tcttcaagct ctttttcgga taccggctcg
     2761 ctgcgaattc ggtcaagttc atagaagaac tctttaagtg agtctccggt cactggagtg
     2821 cgaacttctg cgctggcgcg aaaggagccg gcactgcggc gcgcgtcgag actggagtaa
     2881 gcgccgtagg tatagccctt atcttcgcgc aaattcatga acaaccgtga cgaggcgttt
     2941 gctcccaaaa ctgtatgcat caacaacatc ggaaagtaat ccgggctggt tcgggtaatg
     3001 ccaccgccgt tcgcaatcac aatattcgac tgagccgaac ccggccggtc aacgaggtaa
     3061 gctgagcggg atgatcgcgg cgggggttcc ggaaacttgt ccgcttgcac ggcgccgggt
     3121 ttccagtccg caaacagatt ctccgcccgt tgaattagcg catccggatc aacatcacca
     3181 gcgacgacca gaacggcatt gttcggaaca aactgggaac gatgaaattc gacaagcttt
     3241 tcgcgcgtgg ttgcctctaa cgaatcaata gtgggagcaa tttttgagta agggtgttcg
     3301 ccaaaaataa ttcgtgagac catctccgtt gccaggaagg atggttgggc gcgctgttgc
     3361 cgcagacttt ccttggtgtt ctccctgatt agttttactt cgttctccgg aaatgaagga
     3421 ttcaacgcaa cgtcggccag caaaccgaga atccggtcgt ggaacaccgt cagagatgag
     3481 gccgccacag tcgtgtaatc tgagttagcc ccggcgctca gggtggcacc catctttgct
     3541 acaagatcgg c
//
LOCUS       DASUEX010000067         3035 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_16855, whole genome shotgun sequence.
ACCESSION   DASUEX010000067 DASUEX010000000
VERSION     DASUEX010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3035
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16855"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1394)
                     /locus_tag="VFV61_01150"
     CDS             complement(<1..1394)
                     /locus_tag="VFV61_01150"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEX4899104.1"
                     /translation="MLKQLGRLERTRSIIIVGFAVLMAVSLIVFYAPGRNTNYLEPTK
                     NTEVVAKVNGAEVTVADIALQKEAVQSRYGGQISLAQLGLTNERILDGVIQKKIIEQE
                     AVRLGLAPSQADVAAEIRKQFRDPTTGQYVNLDPDKDPERYRQIAASQSGDVERFEQQ
                     FHDQLAYRNLRTFITSSVSVSDEEVEDNYRRDTTTLNLTYVLVSPEKLAEKISASDDE
                     LRAYYEQHKTDYRFDEPQKKVRYLYISQDKAGAKLQITDKDLRDRYDQMSPENKQAGV
                     KVQQIFLRVARQDLDAQVEQKAKDLIAKLRGTGTQATKEAFAEAARGNSEDPATAKNG
                     GFLNRLVKKNPNKPDALYERAVDMQPGDVSDIPIRYGGNWYILRRDESVPKTFEEAKP
                     ELLASLRNSRSYGVAAKLAERAQARLKETKDIQKVARELAAEANMQPAEMVKETDYIR
                     PGDDVPDIGSNQQF"
     gene            1652..2719
                     /locus_tag="VFV61_01155"
     CDS             1652..2719
                     /locus_tag="VFV61_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002640542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEX4899105.1"
                     /translation="MNSTTWLWIGFSVFILLMLSLDLGLLNRKAHAIKYREAATWSAV
                     WVTLAMIFAGLVFWYQGSQRGLEFLTGYLIELSLSVDNLFVFLLIFSYFKVPARFQHR
                     VLFWGVLGALVMRLTMIFVGATLIKNFSWVIYIFGAFLVYTGIKMFKQEELDIQPEDN
                     PIVRLVTKYIPIERHYDEQKFFTRKNGRLTGTLLLLVLVIVEVTDLVFAVDSIPAIFA
                     ITTNTFIVYTSNVFAILGLRSMYFLLAGVVEKFQYLKMGLAIVLTFIGVKMLVVAVGI
                     HVPIWASLTFVGVVLLSSVAASLIWPKECETGIEVDLPEDFDSPFEDDEPDADASLEK
                     KPRADAPEVEEKEEPIGVDRL"
BASE COUNT          637 a          791 c          780 g          827 t
ORIGIN      
        1 tcgaactgct ggttgctacc gatgtccggc acgtcgtcac caggtcgaat gtagtccgtc
       61 tctttcacca tctcggcagg ctgcatgttg gcttccgccg cgagttccct ggctactttc
      121 tgaatatcct tggtttcctt cagccgtgcc tgcgctcttt cagcgagttt ggccgccact
      181 ccgtaagaac ggctgttacg caatgaagca agcaactctg gtttcgcttc ctcaaacgtt
      241 ttcggcaccg attcatctcg ccgcagaatg taccagttac ccccatacct gattgggata
      301 tcggaaacat cgccgggctg catatccact gctcgttcgt agagtgcatc gggcttgttg
      361 gggttctttt ttacgaggcg attgagaaaa ccgccgttct ttgctgttgc agggtcttcc
      421 gaattaccgc gagcagcctc ggcgaacgct tctttggtcg cctgcgtacc tgtccctcgc
      481 agtttcgcaa tcaaatcctt agccttctgt tccacctgtg catccagatc ctggcgcgcg
      541 actctgagga aaatctgctg aactttcact cccgcctgtt tattctccgg actcatttgg
      601 tcgtagcggt cgcgcaaatc cttgtcggta atttgcagct tcgcgcccgc cttgtcctga
      661 ctgatgtaaa ggtaacgaac ttttttctgc ggctcgtcaa accggtaatc agtcttatgt
      721 tgttcgtagt aagcgcgcag ctcatcgtct gaagcgctga ttttttccgc gagtttctcc
      781 ggcgagacta aaacgtaggt cagattcaga gttgtggtgt cgcgtcgata gttgtcttca
      841 acctcttcgt cagacacgga cacgctggag gttatgaagg tgcgaagatt tcgataagcc
      901 aattggtcgt gaaactgttg ctcaaagcgt tcgacgtctc ccgactgtga cgcggcaatt
      961 tgccggtacc gctcaggatc tttgtcgggg tccagattga cgtactggcc cgttgtcgga
     1021 tctctaaact gcttacgaat ctccgcagca acgtcggcct gcgagggggc aagtcccaga
     1081 cgcaccgctt cctgctcgat gatctttttt tggattacgc catcaagaat gcgctcgtta
     1141 gtgagaccaa gttgggccaa actgatttgg ccgccatagc gcgactgcac cgcctctttt
     1201 tgcagggcga tgtcagcaac agtgacctca gcaccattaa ccttggcgac tacttctgtg
     1261 ttttttgttg gttcgagata gtttgtgttt cgtcctggcg cgtaaaagac aatcaaactg
     1321 accgccatca atacggcaaa tcccacaatg ataatgctgc gggttcgttc cagacgtcca
     1381 agttgtttaa gcatttatta tttacctttc cagcgaaggg cggattgtaa aggagcgggg
     1441 cgcagcactg caacttggga acttagaact tagaactttg tactttgttc tgtttttagg
     1501 cgggtggttt ggagaatgcg atatgtcgga aagaaacttc agcaaagttc caagaacaaa
     1561 ggtccaagtt ctaaaatgtg aatccccaaa gttaacgacc ctgccgcgta cctgacatca
     1621 gacgcccgtc aatggtattt tagtttcctc gatgaacagc accacttggt tatggatcgg
     1681 cttttctgtc tttatcctgt taatgttgag tctggatctc ggactcctta accgcaaggc
     1741 ccacgcaatc aaatatcgcg aagcggccac ctggagcgca gtctgggtga cgttggcgat
     1801 gatttttgcg gggcttgtct tttggtacca gggcagccag cgcggtctgg agtttcttac
     1861 cggctatctg atcgagctct cactatccgt agacaacctc ttcgtcttcc tgctgatctt
     1921 ctcctatttc aaggtaccgg cacgattcca gcatcgcgta ttgttctggg gcgttctcgg
     1981 tgccctggtg atgcgcctga ccatgatctt cgtgggcgcc acgctgatca aaaacttttc
     2041 ctgggtcatc tatatcttcg gcgccttcct cgtttatacc ggcatcaaga tgttcaagca
     2101 ggaagagttg gacatccagc cggaagataa ccccatcgtc cggcttgtaa ctaaatacat
     2161 accgatcgaa cgccactacg acgagcaaaa gttcttcact cgcaagaacg gcagactgac
     2221 gggtacgctc ttgttgctgg tgctggtgat tgtagaagtc acggacctgg ttttcgcagt
     2281 cgactcgatt ccggcgatct ttgcgattac gaccaatacc ttcatcgtct acacctcgaa
     2341 tgtctttgcc atcctgggac tgcgttcgat gtacttcctg ctagctggag tggttgagaa
     2401 gtttcagtat ctgaaaatgg gactcgccat cgtgctcacc tttatcggtg taaagatgct
     2461 ggtggttgcc gtcggtattc acgttccgat ttgggcctca ctgacgtttg tcggagtggt
     2521 cctgttgagt tcggttgccg catcgctcat ctggccgaag gaatgcgaga cgggaatcga
     2581 agtagacttg cctgaggact ttgactctcc ctttgaagac gatgagccgg atgctgacgc
     2641 ttccctggaa aagaagccgc gtgcagacgc tcccgaggta gaggagaagg aagagcccat
     2701 cggcgtcgat cggctttaag gccatcgaaa aggattaatc gacaacatta ggatcctgcg
     2761 ttaaaatgcc ggggcttcag agacctttcg aacgattggc catggctgct ttgagaccac
     2821 ttaccaaact cctccttacg gtgccgcttt tgcgccgcgc agcctagcgc gtcgggagcg
     2881 gcgaatctct acatcttatt gcgacgcttc actgtaattc ggtgaggctt tgtccgtttc
     2941 aggcggaacg attgatctag cctagattgc atcagggacg actcagatga acgaaaagta
     3001 ttttccggaa gtcattgaag cgaagtggca aaaac
//
LOCUS       DASUEX010000068         2145 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1384351, whole genome shotgun sequence.
ACCESSION   DASUEX010000068 DASUEX010000000
VERSION     DASUEX010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1384351"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..797
                     /locus_tag="VFV61_01160"
     CDS             <1..797
                     /locus_tag="VFV61_01160"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX4899106.1"
                     /translation="LDLLGVGISVITAVNIQHLESLNDVVTRTTGVRVRETVPDYFLR
                     RADEVVNVDVSVDTLRTRLRQGKIYDVVKIEQALNNFFRKGNLSALRELALRQVATDQ
                     ATKAQDYREREGLEQAVIPERVMVAIASRGSAKKLLRIGSRIAGRLASDWYAVYVETP
                     NEEMGRIKPEDYAALQENIRFAEELGAKVIRLKSRRVADALIEFAKREGITHVVFGQT
                     ARSRWDILFHGSIINRFLDEVRDATVQVAPLDQKSEVGNQESSVEQ"
     gene            841..>2145
                     /locus_tag="VFV61_01165"
     CDS             841..>2145
                     /locus_tag="VFV61_01165"
                     /inference="COORDINATES: protein motif:HMM:NF024140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEX4899107.1"
                     /translation="MSLRTKLLLSYLVFVAALVVLGGWSAWRLREMGGVSRRIISNNY
                     DSVVAAQEMKESLERQDSAALFALLAAREKSMTQLREHRARFDANFQKAANNITEVGE
                     PEAIEAIRRDRDSYYQMFDAFMAKVSATESSPQRVLREEELSERNEYFTRLEPQFNKL
                     RTECEHLLQLNQRAMLAKSEAAAGVAQLWFYRTLLIAGILVAAGLLLALFLANRIVEP
                     LRQLTATTARMAGGDLDARVTVRSRDEVGVLAAEYNRMAERIRQLRSSDMGKLLVAQQ
                     TTEAAIDSLYDPVIVTDGEGCVTKLNPAAEEIFGSETENTGKHVGEVARDERIAGAVA
                     EALESQRPVAGEGMSSVLPLAVDGSQRAFRLRTTPMRDNSHHLLGAVTILEDITHLRE
                     IDRLKSEFIATASHELRTPLTSVQMGVHLLLEGALGELTDSQD"
BASE COUNT          506 a          544 c          608 g          487 t
ORIGIN      
        1 tgctggactt actgggcgtc ggcatctccg taatcacggc ggtcaatatc cagcacctcg
       61 aatcacttaa cgatgtggta actcgcacca caggcgtacg cgttcgggag accgttccag
      121 actattttct gcgacgagcc gatgaggttg taaatgtcga cgtctctgtt gacacgctcc
      181 gcacgcgtct acggcaaggg aaaatctacg atgtcgtgaa gattgagcaa gcgcttaata
      241 attttttccg aaagggaaat ttatcagcac tgcgcgagct ggccctcagg caagtggcta
      301 cggatcaagc cactaaagcc caggactatc gggaacgcga aggccttgaa caggccgtca
      361 tcccggagag agtgatggtt gcaatcgctt cacgcggtag cgcgaagaag ttgctgcgga
      421 tcggatcacg catcgccgga cgcctggcga gcgattggta cgccgtttat gtcgaaacgc
      481 ctaacgaaga gatggggcgc attaagcctg aagactacgc agcccttcag gaaaacatcc
      541 ggtttgcgga ggagctcgga gcgaaggtca ttaggttgaa gtcgcgacga gtagcagatg
      601 ctttgatcga attcgcgaaa cgagaaggga tcacacacgt agttttcgga caaacggcgc
      661 ggtcacgatg ggacattctt tttcacggtt caatcatcaa ccgttttctt gatgaagtgc
      721 gagatgcaac agtgcaggtg gcacccttgg atcagaaatc cgaagtcgga aatcaggagt
      781 caagcgtcga acaataattt ttctggctgc tgaactctga ctcctgactt ctgtgttgtc
      841 atgagcttac gaacaaaact tttgctcagt tatctggttt tcgttgcggc actcgttgtc
      901 ctgggagggt ggagtgcttg gcggctacgg gaaatgggtg gtgtgtctcg ccgcatcatc
      961 tccaataact atgactcagt ggtcgctgcg caggaaatga aggaaagtct tgagcgtcag
     1021 gactctgcag cgctattcgc tcttttggct gcgcgtgaga aatccatgac tcaactccgt
     1081 gagcatcgag cgcgctttga tgccaatttt cagaaggccg ccaacaacat taccgaggtt
     1141 ggtgagcccg aagccataga ggcgattcga cgggaccgcg atagctatta ccagatgttc
     1201 gatgccttta tggccaaagt aagtgcgacg gaaagttcac cgcaacgagt actgagagaa
     1261 gaagagctct cagagcgcaa tgagtacttc acacgtctcg aaccacaatt caataaattg
     1321 cgcactgaat gtgaacacct gctgcaactc aatcaacggg ccatgcttgc caagtctgag
     1381 gccgccgccg gcgtggccca actctggttt taccgcacgc tgctaattgc gggcatcctg
     1441 gtggcggccg ggttacttct ggcgctcttt ctagcgaatc gtattgtcga gccattgcgt
     1501 cagcttactg cgactactgc aagaatggct gggggcgatc ttgatgcacg agtaacggtc
     1561 cgttcgcgtg atgaagttgg cgtgctggcc gcagagtaca accggatggc cgagcggatt
     1621 cggcaactgc gcagctcaga tatgggaaaa cttctggtag ctcagcaaac taccgaagcc
     1681 gccatcgatt ctctttacga cccggtgatt gtgaccgatg gtgaagggtg cgttacgaaa
     1741 ctaaaccccg ccgcggagga gatatttggt tccgaaactg aaaacactgg caagcatgta
     1801 ggtgaagtgg cgcgagatga gcgcattgcc ggtgctgtcg ccgaagcgct ggagtcgcag
     1861 cgtccagtcg cgggcgaagg gatgtcttcg gttttgcctc tcgccgttga cggttcccag
     1921 agggcctttc gcttgcgcac cactcccatg cgcgacaaca gccatcattt gttaggggcg
     1981 gtcaccattc tcgaggacat tactcatctc cgcgagatag atcgcctcaa atcagagttc
     2041 atagctactg cttcacacga acttcggact ccgttaacca gcgtccaaat gggtgttcac
     2101 ttactgctcg aaggcgcact cggcgagtta actgatagtc aagat
//
LOCUS       DASUEX010000069         4695 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_17035, whole genome shotgun sequence.
ACCESSION   DASUEX010000069 DASUEX010000000
VERSION     DASUEX010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4695
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17035"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1148)
                     /locus_tag="VFV61_01170"
     CDS             complement(<1..1148)
                     /locus_tag="VFV61_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899108.1"
                     /translation="MSSLLILLLCGSFYVAAAKIPVEKVAKSMPREIGGFRLAGTVAS
                     LSDEEIAASQEAHGFRLANSQGVALVGAAYVSPAGEKFRVELVRVERDSDAYSLLTQF
                     AHSARLTEQGKNVQLTRDIGTSSFALPGRIAFFKGTAFVTVSDVARPATNSEKLVEFA
                     SSFAARLETGEGDVPILVKHVPQWEQAQATALYLAGFTSLKGTVPNQPVLEAITSEGD
                     ADAVLTSYGSAQFLLVEFNTPQLAGDNDRAITAKLNELRQQGQPVPTSYRKVGNYSVF
                     VFNAASEAEAQDLINQVEYAQMVTWLGHNPYLLEQAQKDYYETTAGVLVAVVKASGLS
                     LLVCLGIGGLIGAVLFNRRRAQQRSAEAYSDAGGMLRLNIDNITPQGDS"
     gene            complement(1277..1861)
                     /locus_tag="VFV61_01175"
     CDS             complement(1277..1861)
                     /locus_tag="VFV61_01175"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEX4899109.1"
                     /translation="MHCSNCGSYIPPGVRFCAGCGSPANDPEITRLARTQSGMSLEHS
                     GADEIERTIFSVRPTLIFIKLGYLLAVLGAIALVVLLASFQVSPFISIPLGLALLLFP
                     AYFHLKRNMIRYTLTDSKMEIDTGLIARTTRNIPLSKIQDVTVSASIPQRLLGFGDLI
                     VDNASEVGGTIVMQNINKPRHYADLLLRELRRWH"
     gene            complement(2001..2807)
                     /locus_tag="VFV61_01180"
     CDS             complement(2001..2807)
                     /locus_tag="VFV61_01180"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEX4899110.1"
                     /translation="MTKVALVFFVLITSAVSALAQSGSEYTIDNFDFANGVRIETPAA
                     QKPVRTSRRFTRLTSSRISNQAPLIDNNTFAGFDAANSSNALSGFTTGNGEVDAFIVA
                     SGKRNAVDPLLLYSIMHQESTFKRNALSPKGASGLMQLMPPTARRFGVSKIWDPQQNI
                     EGGARYMRFLLDQFGDVQLALAGYNAGEGAVMKYGNRVPPYRETQEYVRRISKRYSLI
                     RDPQTAQFANNLSQQQLAAARQKESAPLTIYEQSVFAVRLPDGRLQLVSQ"
     gene            3220..3564
                     /locus_tag="VFV61_01185"
     CDS             3220..3564
                     /locus_tag="VFV61_01185"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HEX4899111.1"
                     /translation="MKKLLAVSLSLLTVLSLAVMGNSISASARTQTMASTTKKRGPIF
                     RATKEQIKEAQIILKTRGFYNGEATAKLDDATREGLRGFQKAEGLKVTGTMNKVMLEK
                     MGIQLTDKQKAM"
     gene            complement(3772..>4695)
                     /locus_tag="VFV61_01190"
     CDS             complement(3772..>4695)
                     /locus_tag="VFV61_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899112.1"
                     /translation="VFATGAAADGLVLQNVEDLTIEGITFVGSPNLLPDGSLNPSAGS
                     DVFRAINLQGIMRAKIQNNMFYGIAAPAPGAAIIKAVASNLTVEHNQFLGSAACQSGN
                     AYGGVIMTDSQFGVRIVNNSFIDYGTLNGVYYSKTPLVWTMAWVMLMNTNSNVALDSR
                     TSNLVEISNNQFDEGATYAIVNNPDQGHAVTQQVRLAYNTVNVGIVGGYYLSRIKYLD
                     IKGGWAGFRPMGGGNNEVDAITLRSVEFPTIRNFETDSTKSATRLSVDSGTTMLRVVD
                     SKFTTIVNQAKAWRIEDGTKVTSSSQTKFLP"
BASE COUNT         1126 a         1232 c         1248 g         1089 t
ORIGIN      
        1 gaatcacctt gaggtgtgat gttatcgatg ttcagacgca acatgccccc cgcgtctgaa
       61 tatgcttcag cggaacgttg ttgcgcgcga cgacggttga agagcacggc gccaattaat
      121 ccgccgatac ccaaacacac cagcaacgac aatccggagg ccttaacaac cgcgaccagc
      181 actcccgccg tcgtttcgta atagtctttc tgggcctgct ctaatagata aggattgtga
      241 cccagccagg tcaccatttg cgcatattcc acttggttga ttagatcctg agcttccgcc
      301 tctgacgcgg cgttaaaaac aaacacagaa taatttccga ccttgcgata acttgtggga
      361 actggctggc cttgttgtcg cagttcattc agcttggccg taatggcgcg atcattgtcg
      421 ccggcaagtt gtggtgtatt gaactcaacc aataggaact gtgcgctacc gtacgaggtt
      481 agcactgcgt cggcatcgcc ttctgacgtt attgcttcca gcactggctg attcggcact
      541 gttcctttca aagacgtgaa accggcgaga tagagtgcag tcgcttgcgc ttgttcccat
      601 tggggtacgt gttttacgag gatgggaacg tcgccttcac ctgtttccaa gcgcgctgcg
      661 aaggaacttg cgaactctac taacttttct gagtttgttg cgggtctggc gacatcagaa
      721 actgttacga aagccgtccc tttaaagaag gcaatacggc caggcaaagc gaagctgctg
      781 gttccgatgt cccgcgtgag ctgaacgttc ttcccttgtt ccgttaagcg agcgctgtgc
      841 gcaaattgag tcaacaagga ataagcatcg gagtcgcgtt cgacgcgaac cagttcaact
      901 ctaaacttct caccagcagg cgagacgtag gcagctccaa ccagggcaac tccctgagag
      961 ttagccagtc gaaacccgtg tgcttcctga cttgcggcaa tttcttcatc tgaaaggctg
     1021 gccaccgtgc ccgctaagcg aaacccgccg atctcgcgag gcatcgactt ggctaccttt
     1081 tcgaccggaa tcttggccgc tgctacatag aatgatccac acagcagaag gattaacagt
     1141 gatgacaact taagaactcg caaacttgtg ccccggagat cagtattgaa ccggctctcg
     1201 cttagaaacc ggtgtcaggt tggatgttcc ctgattatct ttcagttgtg ccattcaatc
     1261 ccgaaaggat tgcagtttaa tgccagcgcc gtaactcccg caaaaggagg tcggcgtaat
     1321 ggcgcggctt gttgatattc tgcatcacga tagtgccgcc cacttcgctc gcgttatcca
     1381 cgatcaaatc gccaaaaccc agcaggcgct gcggaatgct tgccgaaaca gtcacatcct
     1441 ggatcttgct gagggggatg ttacgagtgg tgcgggcaat caggccggtg tcgatctcca
     1501 ttttggagtc ggtgagtgtg tagcgaatca tatttcgttt gagatgaaag taagcgggaa
     1561 acaaaagcag cgccaatccc agcgggatcg aaatgaacgg tgacacctga aaagacgcca
     1621 atagaacgac cagagctatg gcgccgagta ccgcgagcaa ataacccaac ttaatgaaga
     1681 ttagcgtcgg ccgtacgctg aagatcgtgc gttcgatttc gtccgcgccc gaatgttcaa
     1741 gagacatgcc gctttgggtg cgcgcgagtc gtgtgatttc cggatcgtta gccggggagc
     1801 cacagccggc gcaaaagcga actccgggcg ggatgtacga gccgcagttt gaacaatgca
     1861 ttcgctaacc tcgattatgt cgtcaactag taagcttcct ggagcgttca tcatacctaa
     1921 agagactggg gtgctgaaat acaaaacgag gcggaaccat ctcaggtttc cgcctcgttg
     1981 gccaaaaata agtagacttg ttactggctg actagttgca acctgccgtc cggcaaacgc
     2041 accgcgaaaa cactctgctc ataaattgtg agcggcgccg attctttttg tcgtgccgca
     2101 gccagttgtt gctgactcag attgttggcg aactgggcag tttgcggatc gcgaatcagg
     2161 ctgtaccgct tgctgatgcg tcgcacatat tcctgcgtct cgcggtaagg gggaacgcga
     2221 ttgccatact tcattaccgc gccttcgccc gcgttgtacc ccgccagcgc taactgtacg
     2281 tcaccaaact gatcgagcag gaagcgcatg taacgcgccc cgccttcaat gttctgttgc
     2341 ggatcccaaa tctttgacac tccgaaacga cgcgcggtgg gcggcatcaa ttgcatcagt
     2401 ccacttgcgc ctttgggaga aagtgcgttg cgcttgaagg tcgattcctg atgcatgatt
     2461 gagtagagca acagcggatc aacggcattg cgttttccgg aagcaactat gaaagcatcg
     2521 acctcgccat tgccagtcgt gaagccagac aaagcattgc tgctgttagc tgcatcaaag
     2581 ccggcgaatg tgttgttgtc gattaaaggt gcctggtttg aaattcggga agaagttagg
     2641 cgagtgaacc gtcgagacgt gcgtaccggt ttttgcgctg ccggcgtttc aatcctgacg
     2701 ccgttggcaa agtcaaaatt atcgatggtg tactcgctgc cgctctgagc gagcgcagaa
     2761 actgccgacg tgatcagaac gaaaaagacg agggcgacct tggtcatgtg tcgagtcctg
     2821 ttcgaaggtt atgtgctgcg aaaaatgagc cggagacgtg aggtcttggg gcggttggat
     2881 tcgctgtgac caaccgtccc ggatagtaac acaggggtta aacgattgcc aacactacga
     2941 ctttttcgcg cttttgctca ggaaaaacgg agaaaaggta ggccaaccca ggaagggagt
     3001 gttcagccag gaaggtgaac gcgcagttat gaaggtcggc gtatcggcct ctttgcttca
     3061 cggccccctg ggaaagagtt cttgcaaaga gccggatccg acgcttgcgg tcggaagctg
     3121 gtgtcccggt ctcacggtat ttatcattcc ctcgcgttaa cgaaacctca ttcttttaac
     3181 cgcgcgcttc agcgcgcctc acaaaaagag gaaactaaaa tgaaaaaact gctagcagta
     3241 tctctgtctc tcctcaccgt tctgtcactg gctgtcatgg gaaactcaat ctcagccagc
     3301 gccagaactc aaaccatggc cagcaccaca aagaaacgtg ggcccatctt ccgagccacc
     3361 aaagaacaga ttaaggaggc gcagataatt ctcaagactc gcggtttcta caacggcgaa
     3421 gccacggcga aacttgatga tgcgacgcgc gaaggtttac gtggatttca gaaagcggaa
     3481 ggactgaagg tcaccggaac catgaacaag gtcatgctcg aaaagatggg catccagcta
     3541 accgacaaac aaaaggcaat gtgagcaatc aagcgtctgc cggtggggaa ccgccggtgg
     3601 aaggcgaagg cgcgagtgtg ttcgtggaga tggtcacact cgcgcctctt gtccctattt
     3661 cctcgatgaa ctaataatcg gcaggaagtc ttaagtgact tgcggtccca cacctgggcg
     3721 actggccgtc attgatttgc agaccagagg ttcctccgtt ggaactcttt gctaaggcaa
     3781 gaattttgtt tgcgagcttg aagtgacctt tgtgccgtct tcgatgcgcc acgctttagc
     3841 ctgattcaca atagtggtga attttgagtc aactacccgc agcatcgtgg ttcccgagtc
     3901 aactgagagc cgagtggcac tcttggtcga atccgtttcg aagtttctaa tcgtaggaaa
     3961 ctcaacacta cgcagagtga ttgcatcgac ttcgttattc ccgccgccca tcgggcggaa
     4021 tcctgcccag ccacctttga tgtcgaggta ttttatccgg cttagatagt aaccaccgac
     4081 aataccgaca tttactgtgt tataggccag tctcacctgc tgcgtaacag cgtgaccctg
     4141 gtccggatta ttcacgatcg catacgtcgc gccttcatcg aattggttgt tggagatctc
     4201 gactagattg ctggtgcgcg aatcgagcgc aacatttgaa ttcgtgttca tcagcatcac
     4261 ccaggccatc gtccatacca gcggagtctt cgaatagtag acaccattca gcgtcccgta
     4321 atcgatgaac gaattattaa caatgcgcac tccgaactgg ctgtcggtca tgatcacccc
     4381 gccatatgcg ttgcccgact ggcaggcggc acttcccagg aactgattat gctcgaccgt
     4441 caagttggag gccacggctt ttatgatcgc ggcccctggt gccggcgcgg caataccgta
     4501 gaacatgttg ttctgaattt tggcacgcat aatcccctga agatttatcg cgcggaatac
     4561 gtcgcttcca gctgatggat tcagcgaacc atccggtaac aagtttggcg aaccaacgaa
     4621 agtgattcct tcaatcgtaa ggtcttcgac gttttgcaga accaggccgt cagcggctgc
     4681 gccggtggca aaaac
//
LOCUS       DASUEX010000070         2736 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1154067, whole genome shotgun sequence.
ACCESSION   DASUEX010000070 DASUEX010000000
VERSION     DASUEX010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1154067"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..678)
                     /locus_tag="VFV61_01195"
     CDS             complement(<1..678)
                     /locus_tag="VFV61_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899113.1"
                     /translation="MPKIKPLNKLVNKARDLRAGHKERHRPTGFGFALADSIDYLDAK
                     HWQAVTSQQSLFLAPHYLRVLEHAGPENLRQRYALIFRGREPVAAIAAQMVTISLSRV
                     SQKPRRGRTAAPLEKLQEKMLVCGNLLSWGMHGIAFARNEDSDKLWPAIAEAIYRVRR
                     ADKLSGDTDLVMVKDIPEAHAASATALQRFSYRQLETEPNMVLDISPKWRSYADYLAS
                     LTSRYRKH"
     gene            774..1541
                     /gene="xth"
                     /locus_tag="VFV61_01200"
     CDS             774..1541
                     /gene="xth"
                     /locus_tag="VFV61_01200"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEX4899114.1"
                     /translation="MRIATWNVNSIIARLPHVTRWLKAMEPDVLCMQETKCTDDKFPF
                     EEFANMGYCASVFGQQSYNGVAILARRECADLQRGNYEDQDGAHARLLAGTFDDVRIV
                     NVYIPNGQAVGSEKFAFKLDWMKRLRAFFDNSYQRDMPVILCGDFNVAPEDRDVHNPQ
                     LWQERIMCSQAERAALDEIKRWGFTDTFRMHHEEGGQFSWWDYRAGAFRRNLGLRIDH
                     VWGTKALAKRSVNTWIDIEPRTWERPSDHAPIVAEFN"
     gene            complement(1637..1912)
                     /locus_tag="VFV61_01205"
     CDS             complement(1637..1912)
                     /locus_tag="VFV61_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899115.1"
                     /translation="MQIIGMLVSLGCFICFIMVLIKQFQNAGAVHGIIGIITCTIWTF
                     IWGWMNATNLNIKNIMMIWTLLIVVAIAINLIFGSYYATTMMPTNAP"
     gene            complement(1986..>2736)
                     /locus_tag="VFV61_01210"
     CDS             complement(1986..>2736)
                     /locus_tag="VFV61_01210"
                     /inference="COORDINATES: protein motif:HMM:NF025580.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4328 domain-containing protein"
                     /protein_id="HEX4899116.1"
                     /translation="YNPPPHLRYVPTQFRANFVCILLLANAAISFASTLLSVLELLWP
                     GELFSEGFEDNPVTLVLALLTLALAIVQIVVYITTVVVFLMWLYRAHENLAAFGIPRN
                     QMQYSSAWAVGSFFVPFASLIIPYRAIRELWQKSVPNYVTMFSTLSPPRFFPVWWGFW
                     ITSNMADQAYLRLSWREQAPAEVVEVIGAIGGILGIVAALLAIKVVREIQRQQVETSK
                     LARAQPDFTPPPGPPQFEQPGNLSAPSPSNS"
BASE COUNT          668 a          675 c          774 g          619 t
ORIGIN      
        1 atgttttcga tatctcgaag taagactggc aaggtagtct gcatacgacc gccacttggg
       61 cgaaatatcc agaaccatgt tgggttcagt ttcgagttgt cgataagaaa accgctgtag
      121 cgcggtcgca ctcgctgcat gcgcttccgg gatgtccttg accataacca aatcagtatc
      181 acctgagagt ttgtcagcgc gtcggactct ataaattgcc tctgcgattg caggccacaa
      241 cttgtcgcta tcttcgttgc gtgcgaaagc aatgccgtgc attccccagg agaggaggtt
      301 gccgcacacc agcattttct cttgaagctt ttccagcggc gcggccgtcc ggccccgtct
      361 tggcttctgg ctcacacgcg agagcgagat ggtcaccatt tgagccgcaa tggcagcgac
      421 cggttcacgg ccgcgaaaaa ttagagcata gcgttggcgc aagttttcgg gtccggcgtg
      481 ttcaagcacg cgtagataat ggggcgccag aaagagagat tgttgggagg tgacggcctg
      541 ccagtgcttc gcatccagat aatcaatgct gtcggcaagc gcaaagccaa agccagtagg
      601 ccgatgccgt tctttgtgtc ccgcgcgcag gtcgcgggcc ttgtttacca gtttgttgag
      661 tggtttgatt ttgggcattc ggttggtctc gggagcaaga gtataaatga actctaagcg
      721 gccggaaata tgaagcctca gcgcgatgct aacgttattt ggaaacaatc actatgagaa
      781 tcgctacctg gaacgtcaat tcaataattg cccgattgcc gcatgtcact cgctggctga
      841 aggccatgga gcccgacgtg ctttgcatgc aggagactaa atgtacggat gacaagtttc
      901 cctttgagga gtttgcaaac atggggtact gcgccagcgt gtttggtcag caaagctaca
      961 acggcgtggc aatcctggcg cgccgcgaat gcgctgactt acaacggggt aattatgaag
     1021 atcaagacgg agcacacgcg cgcttgttgg cgggcacgtt cgatgatgtc cgcatcgtca
     1081 atgtctacat tcccaacgga caggcggtcg gctcagaaaa gtttgcgttc aaactcgatt
     1141 ggatgaaacg actgcgcgcc ttctttgaca attcctatca acgagacatg ccggtgattc
     1201 tttgtgggga ttttaatgtg gcccccgagg atcgagacgt tcacaatcct caactctggc
     1261 aggagcggat tatgtgcagc caggctgaac gtgcagctct ggacgaaatc aagcgttggg
     1321 gcttcactga cacattcagg atgcatcacg aggagggcgg tcagttctcc tggtgggact
     1381 accgcgcggg tgcctttcgg cgcaaccttg gcttgcgaat cgatcatgtc tggggaacaa
     1441 aagcgttggc aaagcgcagc gttaatacgt ggattgatat tgaaccgcga acctgggagc
     1501 ggccttcaga tcatgcgccg atagtggctg agtttaattg agagatcacg aacggggagt
     1561 caggcgagga caccgacgct gcgcccgctg gaagaccttc cagctctggc gcagcgcgag
     1621 agaaatgtga gcagcgttaa ggggcgtttg tcggcatcat agtggttgcg taatacgagc
     1681 cgaagatcaa gttgattgca attgctacca caatcaacag agtccagatc atcatgatgt
     1741 tcttgatgtt cagatttgtg gcattcatcc aaccccaaat gaatgtccaa atggtgcaag
     1801 tgattattcc aataatgcca tggaccgcgc cggcattctg aaactgtttg atcagcacca
     1861 tgatgaaaca gataaaacat cccagcgaaa ctagcattcc aattatctgc atggtccctc
     1921 cagttaagtg gttgatgttc gggtaagcgg acgcacaata atgccgttta ggaaaactgt
     1981 caactttatg aattggaagg tgaaggagcg ctgagatttc ccggttgctc aaactgtggc
     2041 ggcccaggcg gcggtgtgaa gtcgggctga gcccgggcca gcttactggt ctcgacctgc
     2101 tgacgttgaa tctctctaac gactttgatt gcgagcaagg ccgcaacaat tccgagtatg
     2161 ccaccgatcg ctccaataac ttccaccact tctgcaggag cttgctcgcg ccacgacaat
     2221 cgcaaataag cctggtctgc catgttcgag gtgatccaga aaccccacca gacaggaaag
     2281 aatcgcggcg ggcttaaagt actgaacatt gtcacgtaat tcggtacgct cttctgccac
     2341 agctccctga ttgctcgata gggaatgatg aggcttgcaa agggaacaaa aaaactcccg
     2401 acagcccagg ccgatgagta ctgcatttga tttctcggta ttccaaatgc cgccagattc
     2461 tcgtgggcgc gatagagcca catgaggaag accacgaccg tggtgatgta gaccacgatt
     2521 tgtactatcg ccagcgccag ggttagtagc gccaggacga gtgtgaccgg attgtcttca
     2581 aatccttcgc taaacaactc accgggccaa agcaattcca ggacggagag cagagtggaa
     2641 gcaaagctga tggctgcatt ggcaagcagc aggatgcaaa cgaagttcgc gcgaaactgc
     2701 gtggggacat agcgcaggtg tggaggcggg ttgtag
//
LOCUS       DASUEX010000071         3249 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_590142, whole genome shotgun sequence.
ACCESSION   DASUEX010000071 DASUEX010000000
VERSION     DASUEX010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3249
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_590142"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(142..1524)
                     /gene="hydA"
                     /locus_tag="VFV61_01215"
     CDS             complement(142..1524)
                     /gene="hydA"
                     /locus_tag="VFV61_01215"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HEX4899117.1"
                     /translation="MKTIIRNGRVVTATDDYHADVLIDGETVSVIGAKLEMEVDKTID
                     ASGKLVIPGGIDPHTHMELPFGGTESSDDFRTGTIAAAHGGTTSIIDFAVQYKGQSLI
                     EGIDNWHKKAEGKTAIDYGFHLITTELEDSQIEELHTAMDEGVTSFKMFMAYPGVFLV
                     DDATIFRGMLAAGERGGLICMHAENGIVINEIIKHALAKGHTAPKYHALTRPTVAEAE
                     GVHRAIAIAEMAESPVYIVHLSCTDALNQVRQARDRGIPAFAETCPQYLFLSLDDYDE
                     PGFGGAKYVMTPPLRDKANQAELWKGLKSDDLQVISTDHCPFCMKEQKELGLGDFTKI
                     PNGAPGVEHRVSLIYNGGVVENRISLNRFVELTSTAAAKMFGLFPKKGTIAVGSDADI
                     VIFDPDKEQTLSVNTHHMNVDYSAYEGRKIRGVVQTVLSRGRVVIDNGEYKGKAGDGQ
                     FLKRGTCVSM"
     gene            complement(1617..3095)
                     /locus_tag="VFV61_01220"
     CDS             complement(1617..3095)
                     /locus_tag="VFV61_01220"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX4899118.1"
                     /translation="MDTYRNYIDGKWIESTSTRTIQNVNPANTDDVIGTIRQATRAEA
                     KAAVDAAAENFAAWRATPAPTRGRIVGRAARLLEENKEDLAQLLTREEGKTIAESRGE
                     LQRSINVADFCAGEARRLNGETIQSELPANFAYTLKQPLGVVACVTPWNFPVAIPVWK
                     IAPALVAGNTVVFKPASLTPATAVHIVDLFEAAGLPPGVLNLVLGSGSDAGDEIINHP
                     AVKAVSFTGSNQVGIRLYEQVSRRGGKVQCEMGGKNPVVILEDADMELAVESTAQGAF
                     GSSGQRCTATSRAVVVNKVADEFVDRLAKRAASMRIGNGADPTTEMGPSVDESQFKTV
                     LSYVEIGREDGATLVCGGQRAQGDELNKGYFVQPTVFDHVTPDMRIAREEIFGPVLSV
                     LRVKDFEDAMRVANDCEYGLSSSIFSNDATKIFRFVDEIETGMTHINSPTTGGEAHIP
                     FGGTKGTGIGDREQGSTALDFYTELKVVYVDYTGRKREGNLY"
BASE COUNT          707 a          895 c          878 g          769 t
ORIGIN      
        1 gcaccattaa cggtaataag cgcacgatcg gttccactct gggccagcgc ggatgtggtc
       61 gacagcgcta caacagcaac tagcagcaat atgttcttaa tcatgtcttt aattctctga
      121 gcgtaggagc ggcttcggtc gttacataga aacgcaggtg ccgcgtttca agaactggcc
      181 atcgccggcc ttacctttgt attcgccgtt atcgattaca actctcccgc gagaaagcac
      241 cgtctggaca acgccacgaa tcttgcggcc ttcataagcg ctgtaatcga cattcatgtg
      301 gtgagtatta acgctaagcg tttgttcttt gtcagggtca aagatgacga tgtcggcgtc
      361 ggagcctacg gcaatcgtgc ccttcttcgg aaacaagcca aacattttcg ctgccgccgt
      421 agaagttagt tccacgaagc gatttaggct aatgcgattc tcgaccacac cgccgttgta
      481 gatgagtgag acacggtgct caacaccggg cgccccattg ggaatctttg taaagtcgcc
      541 gagaccgagt tctttctgtt ccttcataca gaagggacag tgatcagtgg agatcacttg
      601 cagatcgtca ctcttcaacc ccttccacag ttctgcctga ttcgccttgt cgcgcagagg
      661 tggcgtcatc acatatttgg ccccgccaaa tcccggctcg tcgtaatcat cgagagagag
      721 gaagagatac tgcggacatg tttccgcaaa tgctggaatg ccacgatcgc gcgcctggcg
      781 cacctggtta agtgcatcag tgcatgacag gtgaacgatg tagaccggcg actccgccat
      841 ctcagcgatg gcaattgcgc gatggacacc ctcggcctca gcgacagtgg ggcgagttaa
      901 cgcgtgatac ttcggcgccg tatggccctt ggcgagagca tgtttgatga tctcgttgat
      961 gacaattcca ttttccgcgt gcatgcagat caggccaccg cgctcacccg ccgcaagcat
     1021 ccctcgaaag atcgttgcgt catcaaccag gaagacaccg ggataagcca tgaacatctt
     1081 gaaactggta acaccttcgt ccatggcggt gtgaagttct tcaatctggc tgtcttccag
     1141 ctcggtcgta atcaggtgga aaccgtagtc gatggcggtc ttgccctcgg ctttcttgtg
     1201 ccagttgtcg ataccttcaa tcagcgattg gcctttgtac tgcactgcaa aatcgataat
     1261 gctggtggtg ccgccgtggg ccgcggcaat tgtgccagtg cggaagtcgt cggacgattc
     1321 agtgccgccg aagggcaact ccatgtgggt gtgcggatcg atgccaccgg ggatcaccag
     1381 ttttccggaa gcgtcgatag tcttgtcgac ttccatttcg agcttcgcgc caatgacgga
     1441 gacggtttcg ccgtcgatga gcacgtcggc gtgataatcg tcagttgcag tgaccacgcg
     1501 accattacgg atgatagttt tcatttttgt gaataaatta aaggggtgga cccgggtgtc
     1561 cacccacttc ataaggggtc ggccgggggc ggacacggcg gtccgcccct gcaatcctaa
     1621 taaagatttc cttctctttt acggccggtg taatcaacgt acacgacttt gagttcggtg
     1681 tagaaatcga gggcggttga gccttgttcg cgatcgccga tacccgtgcc cttggtccca
     1741 ccgaaaggaa tgtgtgcttc gccgccggtc gtgggggaat taatgtgcgt catgcccgtc
     1801 tcaatttcat caacaaagcg gaagatcttc gtggcgtcgt tggaaaaaat cgacgacgag
     1861 agaccatact cgcagtcatt ggctacgcgc attgcatctt caaagtcttt tactcgcagg
     1921 actgacaaga ccggaccgaa gatttcctca cgcgcgatgc gcatgtctgg agttacatga
     1981 tcgaagactg ttggttgcac gaaatatcct ttgttcagtt cgtcaccttg cgcgcgttgg
     2041 ccgccgcaaa caagagtggc gccatcctcg cggccaattt cgacgtaact caatacagtc
     2101 ttgaactgac tctcatcgac actgggaccc atctcggttg tcgggtccgc cccgttgccg
     2161 atgcgcatgg aagccgcgcg cttcgccagg cgatctacaa actcgtcggc gactttgttg
     2221 accaccaccg cgcgactggt cgcggtgcat ctttgccctg atgatccaaa ggcgccctgg
     2281 gcagtcgact ccacggcgag ttccatgtcg gcatcttcca gaatcacaac aggattcttg
     2341 ccacccattt cgcattgcac tttcccgcca cgccgagaca cctgctcgta gagacggatg
     2401 cctacctgat tcgaaccggt aaaggaaacg gccttcactg cgggatgatt gatgatctcg
     2461 tcacctgcat ctgagcccga acccaacacc agatttagaa ctccgggagg gagtcccgct
     2521 gcttcgaaaa gatcgacgat gtgaacggcc gtcgccggtg ttagtgaggc gggcttgaac
     2581 acaactgtgt tgccggcgac caaagccggt gcgattttcc aaactggaat cgccaccggg
     2641 aaattccacg gagtgacgca ggctactacg cccagcggtt gtttgagcgt gtaagcaaag
     2701 ttggcaggga gctccgactg aatcgtttca ccatttaagc ggcgcgcttc acccgcgcag
     2761 aagtcagcca cgttgattga gcgttgcaat tcaccacgcg actctgcaat tgtcttcccc
     2821 tcttcgcgtg ttagaagttg cgctagatct tccttattct cttccaacaa acgcgcagcc
     2881 cgtccgacga tgcggccgcg cgtaggcgct ggcgtggcgc gccaggcggc aaagttttct
     2941 gctgcggcat cgactgccgc ctttgcttcc gcgcgggtag cctggcgaat cgtgccgatc
     3001 acgtcgtctg tgtttgctgg atttacgttt tgaatggtgc gggttgaagt ggactcaatc
     3061 catttcccgt cgatgtagtt gcgataggta tccatgtatc aatctcgaca aacttaggtt
     3121 tgtcgtttgt cattactgat aagaagagat ggggcaaaag cgacggacta gagttgtcag
     3181 acgaacaagt ggatgactct agcgagcccg taatatacca tacgctctag aacatgccag
     3241 ccacccaaa
//
LOCUS       DASUEX010000072         3136 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_590205, whole genome shotgun sequence.
ACCESSION   DASUEX010000072 DASUEX010000000
VERSION     DASUEX010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3136
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_590205"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="VFV61_01225"
     CDS             <1..283
                     /locus_tag="VFV61_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899119.1"
                     /translation="AESQAAGREERQGALQGDDEYAATGIGRSVRNDVRFVDMDLESR
                     PVNEVNIRYEYYGALVRLGILPQKYPRIDPLRRREGATGFEDRRFSPEP"
     gene            complement(393..1811)
                     /locus_tag="VFV61_01230"
     CDS             complement(393..1811)
                     /locus_tag="VFV61_01230"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEX4899120.1"
                     /translation="MSLSRVVAATVLVAGSVLSLSVASVAQSAKDGTLSTIRRTDSRD
                     SNEKLIRLSPAEHLRRANVYMSNRAFEEAREHWRALISFYPNDPAVAEALLGIGRSYQ
                     QSRRYAEASVTYDELFKRFSSTKEGREGLNFNAAALLRMGKPGEAVEKYAEYIRLFPN
                     GERIDTAHLNIIDTLREAGRPSEAITWVERTRQRFAGTVTETNAAFARLRLDVAESNW
                     KHAVATADELGRLTFGKGVLTTRAEISYLKAYSLERLGNRREAFNTYAAINDAPESYY
                     GGLARERLANLSSPDAQGLVKDRSQRFHSQVSSLADSYPAPYRAAIVKAAKARKLDPR
                     FILAIIKQESVFKPLAKSPAGARGLLQLTIDAAQKYAVAARLNQLRESDLYRPETSIV
                     LGSEYLAELTRMFPNSLEPVAASYNGGEDNVARWVKRARHNDPGVFTAEIGFDETKTY
                     VQKVMTNYRIYRELYTADLSPR"
     gene            complement(1831..2253)
                     /locus_tag="VFV61_01235"
     CDS             complement(1831..2253)
                     /locus_tag="VFV61_01235"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX4899121.1"
                     /translation="MSLGESKAVIHFAQDDLFIGITPSNHALTLDTDHERASAPTPVE
                     LLLVALGSCTAVDVISILKKKREQVSAYRVEVRGERRADHPRSYTRMEVHHVVTGREI
                     SEKAVSQAIELSETKYCSVAATLRPTAEIVSTFEIIEG"
     gene            2579..>3136
                     /locus_tag="VFV61_01240"
     CDS             2579..>3136
                     /locus_tag="VFV61_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899122.1"
                     /translation="MSSFRRAIAIIFALGVLLAGFTAMAQQQRTYRGTYQSVRNLINR
                     IDSRSTTFRNTLDTALDQSQLDGTNAEDNINQFVRDYDASVDRLRERFEARQSTAADA
                     QEVLNRAALIDRFVNRRRLDARVQQDWANVRLQLNQLASVYNVTWPRLGRGRITNPPY
                     PSTSNNVLTGTYRLDASRSDDPREAA"
BASE COUNT          698 a          846 c          867 g          725 t
ORIGIN      
        1 ggcggagagt caggctgcgg gaagggaaga acgccagggc gcgttgcaag gtgacgacga
       61 gtacgcagcg accgggattg gacgttccgt aagaaatgac gtgcgcttcg tcgacatgga
      121 cctcgaatcg cgacctgtta atgaggtcaa cattcgttac gagtactacg gtgccctggt
      181 gcgtttgggg attctgccgc agaagtatcc gcgtatcgat cctttgcgca gacgagaagg
      241 ggcgacaggg ttcgaagacc ggcggtttag cccggagcca taattgattg gcgattgcta
      301 attctgctga attgttcggt gccgaccagt tacgaggatt gagaccgtca agtgaaattg
      361 acctatcgtc caattggcaa tcggcaatat aattatctag gcgagagatc ggcggtgtag
      421 agttcccgat agatgcggta gttggtcatt accttttgca cgtaggtttt ggtttcgtca
      481 aaaccgatct cagcggtaaa gacaccggga tcattgtggc gcgctcgctt cacccagcgc
      541 gccacattgt cttcgccgcc gttgtaagag gcggcaaccg gctcgagact gttgggaaac
      601 attcttgtca actcggcgag gtattcgctg cccaacacga ttgaagtctc gggtcggtag
      661 agatcgcttt cgcgcaactg attgagccgg gctgctacgg catacttctg ggcggcatcg
      721 attgtgagtt gcaacaaccc gcgcgcgccg gcaggagact tcgccagcgg cttgaagacg
      781 ctttcttgct tgataatggc gagaatgaaa cgcggatcga gtttcctggc ctttgcggct
      841 ttgacgatcg ctgcccggta tggagcggga tagctgtccg ccaacgaact cacctgcgaa
      901 tgaaaacgtt gcgagcgatc cttaactaac ccctgcgcgt cagggctgct caggttggca
      961 agtcgctcgc gagcaaggcc gccgtagtaa gactctggcg catcattaat cgcagcataa
     1021 gtattgaaag cctcgcgccg gtttcccagt cgctccaggc tataagcttt gagatagctg
     1081 atctcagcgc gagttgtcag cactccctta ccgaacgtaa gccggcctaa ctcatctgca
     1141 gttgccactg catgcttcca attactttcg gcgacgtcca ggcgcagcct cgcgaatgcc
     1201 gcgttcgttt ccgtgactgt gccggcaaat cgttgcctgg tgcgctccac ccaggtaata
     1261 gcttcgctcg gacgaccagc ctcgcgcaga gtatcaatga tgttcagatg cgccgtgtcg
     1321 atccgctcac cgttgggaaa gagccggata tattccgcgt acttttccac cgcttcgcca
     1381 ggcttaccca tgcgtaacaa cgcggcggcg ttgaagttga gtccttcgcg cccttctttc
     1441 gtagaactga atcttttgaa taactcgtca taggtgacag atgcttcggc atagcgtcgc
     1501 gattgctggt acgacctgcc aattcccagc aatgcttccg cgaccgccgg atcgttagga
     1561 tagaaagaaa ttaatgcccg ccaatgctca cgcgcctctt caaatgcgcg gtttgacatg
     1621 tagacgttgg cgcgacgtag gtgttcggcg ggagagagcc ggatcaattt ctcgttgctg
     1681 tcccgagaat cggttcgcct gatggtgctt aacgttccgt ccttcgcgga ctgggcaact
     1741 gaagcaaccg acagcgacaa gacactcccg gctaccaaca ccgttgcagc taccactctt
     1801 gataacgaca caatagctcc ccctattcaa ctacccttcg atgatctcaa acgtggaaac
     1861 gatttccgcg gtcggccgca aagtcgcagc gacgctgcag tatttcgttt ccgaaagttc
     1921 gattgcctga gaaacggcct tttctgagat ctcacgcccg gtgactacat gatgcacttc
     1981 catccgagtg taactgcgag ggtggtcagc acggcgctcg ccgcgaactt ccactcggta
     2041 ggcgctcact tgctctcgtt tcttcttcag aatgctgatc acgtccactg cggtgcagct
     2101 ccccagcgcg actaacaaga gctccacggg tgtcggcgcc gaagcccgct catgatcggt
     2161 atcaagcgtg agcgcgtggt tcgatggggt gatgcctatg aacaggtcgt cctgagcaaa
     2221 gtggatcaca gctttagatt ctcccaagct cattctggcg gccctccgcg ctcagaatac
     2281 catgggcagt ctgccgcgcc ggccttaaac gtaggtgcga aaccgtgaaa ccgaagcacg
     2341 aaaaagaatg agagcgccgt aactttgtaa tgcaaattgt taaacaaatg tactgttttg
     2401 cttgtttctg ggtgcagttt gcgcggaagt actggtcact ttggcgtctt ctgaaggacc
     2461 aactgggtgt ttcggtccat tggcacaagc cttgtctagt atcagcgtgt tctcccggcc
     2521 aaagaacgac ctcgggcagt atccttagcc tattacggcc acactggagg aaaagtatat
     2581 gagcagtttt agaagagcga ttgcgataat tttcgcactc ggagttttac ttgcgggatt
     2641 taccgcgatg gcacaacagc aacgtaccta tcgtggaact tatcaatcag ttagaaattt
     2701 aatcaatcgg atcgatagcc gttccacaac ctttcggaac acgttggata cagcactgga
     2761 tcaaagccag ctcgacggaa ccaacgccga ggacaacatc aaccagttcg tgcgtgacta
     2821 tgatgcgtcc gtagatcgac tgcgggaacg ttttgaagct cgccaatcaa ccgctgcgga
     2881 tgctcaggaa gttttaaacc gggcggcgtt aatcgatcgt ttcgtgaacc ggcgccggct
     2941 ggatgcacgc gtgcagcagg actgggctaa cgtgcgtttg cagttgaacc agctggcaag
     3001 tgtttataac gttacctggc caagactggg acgcggacgc atcacgaatc cgccttaccc
     3061 ctcgacttca aataacgtct taaccggaac ctatcgcctg gatgcgtcta gaagcgacga
     3121 tccgcgggag gctgcg
//
LOCUS       DASUEX010000073         6352 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_17471, whole genome shotgun sequence.
ACCESSION   DASUEX010000073 DASUEX010000000
VERSION     DASUEX010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6352
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17471"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="VFV61_01245"
     CDS             <1..660
                     /locus_tag="VFV61_01245"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX4899123.1"
                     /translation="VESLCETLSYCNLTWELVLVGNYIEGSADETPVVVNALAQTRSN
                     IKTVVRPKVGMMGWDMRLGLNAARGEYIGVIDGDAQFPPESVVACLLKAKTQNLDLTK
                     TYRVRRDDGFHRRLISFGYNLLFKLLFGLQIRDVNSKPKVIRRSKYEQLHLESDDWFA
                     DAEIMIRAQEAGLSIGEIPVHFLVNDTRGSFVKPAAILEFMINLFKYRFGRRKPNIPK
                     Q"
     gene            complement(680..2053)
                     /locus_tag="VFV61_01250"
     CDS             complement(680..2053)
                     /locus_tag="VFV61_01250"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX4899124.1"
                     /translation="MSRQLRNRLAIFMMIFLLAFSVRLLTMQFMKMHLHDASWFQIGS
                     YQMFEKHAENILSGRERPFWIDDPSRTDLVQYPPAFPWWIALIFRVSGDHSVYAVQRV
                     QWVLDLFLSMFLITGIAVTAFGWRAAIAASFLAAVSPLLAMYGAWPSSDAPTSWLVLG
                     ATWMLLLAAKTSNVRWAIGSGLMLGVACWFRVNPLFLSVTWAFGLLLLLPASWRERVR
                     LSLALAMFAILLVVPITLRNALVFHEFLPNGLNVGVNLWEGLGETDRGVAAGMVLGDQ
                     FMLEIERKELNLPPEYPITLAYPDGIRRDRKRVRDSLQVIAANPLWYAGVMGRRAVRL
                     LKLAGEPAPYQGSAGINVTSKKSLTPAWQGGVLAILVDGLGMAQSVIRYSILLLLLPG
                     VLLALRKNRLGSTLLLITVFYYLLTCSVGHTEMRYALPMYGVLFVFAGVAVAGMIEAI
                     KRRKASV"
     gene            complement(2228..3073)
                     /locus_tag="VFV61_01255"
     CDS             complement(2228..3073)
                     /locus_tag="VFV61_01255"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX4899125.1"
                     /translation="MFSSKPEPQRSNSFLAEEKQRAREQWGQDPCGAENDRENEFGTR
                     QYFDEIERVRYQEYAPWMPELMGFNEFAGARLLEVGCGMGTDLMQFARGGAVCTGMDL
                     TPRSIAITRQRFALYGQRTNLLIGDGERLPFADATLDVVYSNGVLHHTPDTAGAICEL
                     HRVLKPGGTAKVMIYHKNSLNYWGEMMLHRGVFRGELFRGRSPEEIMSRWVEYSEHGA
                     RPLVKAYTRAEAQEMFKDFNGVKIQTEQLIRAELPVVGRFIPEALFRSMRKSVGWNLI
                     ITAVK"
     gene            complement(3217..4863)
                     /locus_tag="VFV61_01260"
     CDS             complement(3217..4863)
                     /locus_tag="VFV61_01260"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX4899126.1"
                     /translation="MRVILVVLSGDARRAQQRVSDILPAATIETIPRSEIENGNLTSR
                     LRALRRHQPEVFAVATERLEWQRGQDLFMLFGALAGAREVLLIDAHGGMRRQDSRGSL
                     LLKGMSRVARESIVSSGVISRSRRELVELEQEIQHRKTLAPVTTKSTSNHPRITYLRA
                     TPGPGTQAGGASSHIKGVIGGLVELGANVELVSNDALAGFDNSNVKLTLIRPEPLGTT
                     RAVFDLHNNEVFTSGALAVVQPNPPDFIYQRYARFSWAGVIVSLRTQRPLFLEYNGSE
                     VWVGKHWDRVSMLDLLERYERLNLAAAARVFVVSEVERKNLERVGIAPEKIIVNPNGV
                     DVKAFRPNVGGLEKRAELGLRSEELLVGFVGTFGPWHGVLVLAEAIKLIPANVPVRFL
                     IVGTGALHGEMKKLLASEEETGRVVFYGSVEHDDIPALLDACDVLVSPHVPLEGGAEF
                     FGSPTKLFEYMAMAKAIVASRLGQIAEVLKHEETALLVSPGNAQELSAAIIRFVESRS
                     LRQTLGAAARSTAVANHTWTRNAQNILDAYQAWRDRTRET"
     gene            complement(4863..>6352)
                     /locus_tag="VFV61_01265"
     CDS             complement(4863..>6352)
                     /locus_tag="VFV61_01265"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HEX4899127.1"
                     /translation="GQRKRGDVVVWREVMMSKDVSVSENDEAAALPDAASPRPRVPPS
                     PSHRVPASPPLSPAAKFSTHVAWTLATRVLMVGNSVVAGIIVARWLGAEGLGQLAVIN
                     VTIATLVQLASFGLPSANTYFIAQDKLQLRAAAMNSLIFALLIGTGLALVLTGVAKLR
                     PEWFGFIPPKLFAIAAISLPFQLLTFIGLNVFLAVGRITQFNLIDLAGQTFVLINAVI
                     ALIVMQAGLWVLVSLNTISSVLIGVLLIVLVGFVAARLNNPLPWRTDFPLFRRMLGYG
                     FKFHISILAGALIFRADLLVVNHFRGAAEAGVYSVTSQVALMLMLLPGVIATLLFPRV
                     AAEPDLSGETTCLVSRHTALLLLFCCLAAIPLSYVLPLLYGAAFADVTVQLLILLPGV
                     YLVGIESVLVQHLNAIGLPRTIPLSWIATLIGNIVLVFVLVPRFGARGAAIASTISYA
                     LIFFLVAHYFTAQTGRPLSDALLLRGSELRRLLTLRSFRSVRGTL"
BASE COUNT         1585 a         1780 c         1638 g         1349 t
ORIGIN      
        1 gttgaaagcc tgtgcgaaac cctgtcttat tgtaatttga cctgggagct ggtgttagtc
       61 ggcaattaca tcgagggctc ggctgacgaa acgccggtcg tagtgaacgc gctcgcccaa
      121 actcgctcga acatcaaaac cgtcgtgcgg cccaaagtcg gaatgatggg ttgggacatg
      181 cgtctgggcc ttaacgctgc gcgcggggaa tacattggcg taattgacgg tgatgcgcag
      241 tttccaccag aatcggttgt tgcctgtttg ctgaaagcca aaactcagaa tctggattta
      301 actaagacct accgcgtgcg gcgcgacgat ggttttcatc gtcgtctgat ttcattcggt
      361 tacaatctgc tgttcaagct actgttcggt ttacaaattc gtgatgtcaa ttcaaagcca
      421 aaagtcattc gccgctctaa atatgaacag ctccatttgg aatcagacga ttggtttgct
      481 gatgctgaga tcatgattcg cgcgcaggaa gccggcctaa gtatcggtga aattccagtt
      541 cacttcctag tcaacgacac ccgtggctcc ttcgtcaagc cagccgcaat tcttgagttt
      601 atgattaatc ttttcaaata tcgatttggc cgccgcaaac cgaacatccc aaaacagtga
      661 agtggttttt gcaagcttgc tacacggaag ccttgcgacg cttaatggct tcgatcatcc
      721 ctgcaacagc aacacccgca aaaacaaaaa gcacgccata catcggaaga gcgtatcgca
      781 tttctgtatg acccactgaa catgtaagca ggtaatagaa cacggtgatt agcaacaagg
      841 tcgagccaag tctgtttttg cgaagagcga gcaacacgcc gggcagcagc agcaagagaa
      901 ttgaataacg gataacgctc tgcgccattc ctaagccgtc gactaatatc gcgagtacgc
      961 caccttgcca tgcaggcgtg agggatttct tgctagtcac attgattcct gcactgccct
     1021 gataaggtgc gggttcgccg gcaagcttta ggagacgtac tgcgcgacgt cccataacgc
     1081 ccgcgtacca aagaggattt gctgcgatca cctgcaagga atctcttacc cgtttgcgat
     1141 ctcggcgaat accgtcggga taagccagtg tgatcggata ttccgggggc aggtttagtt
     1201 ctttgcgctc aatttccagc atgaattgat cgcccaacac catacccgct gcaacgccgc
     1261 ggtcagtctc ccctaagcct tcccatagat tcacaccgac gttaagaccg ttaggcagga
     1321 actcgtgaaa gaccagggca ttgcgcagcg ttatcggaac taccaataag attgcgaaca
     1381 tcgccagagc caaacttaga cggacgcgtt ctctccacga agcaggaagc aacagcaaca
     1441 aaccgaaagc ccatgtcaca ctcagaaaca ggggattgac ccggaaccag cacgcaactc
     1501 ccagcattaa gcccgagccg atcgcccatc ttacgttcga agttttcgcc gcaagcagaa
     1561 gcatccatgt ggctcccagg accaaccagc ttgtgggcgc gtccgaagag ggccaggcac
     1621 cgtacattgc gaggagcgga gaaacggcag ccaggaaact cgctgctatg gcagcgcgcc
     1681 atccaaacgc tgtgacagcg atgccggtga tgagaaacat cgaaaggaat aaatcgagta
     1741 cccactgcac gcgttgaact gcataaactg aatggtcccc gctcacacga aagatcagcg
     1801 caatccacca gggaaaagcc ggaggatact ggaccaaatc ggtgcgcgag ggatcgtcga
     1861 tccaaaacgg ccgttcgcgt cccgaaagaa tattttccgc atgcttttca aacatctgat
     1921 acgaaccgat ctggaaccag gaagcatcgt gcaagtgcat tttcatgaac tgcatcgtca
     1981 gcaaacgcac gctgaaggcg agcagaaaaa tcatcatgaa tatcgcaagg cgatttcgta
     2041 gctggcgcga catgccccgc caccgccggc gggagtttgc tggtgagact tctcgagaga
     2101 gactagtagg cggttgtaac attgccgatt gcccaatgtc ccttgcccaa tgccgagttt
     2161 agattgtcga ttctaatgtt tcctgtactc gtaatcggaa atcggcaatt ggcaatcgcc
     2221 aatcccttca tttaaccgca gtaataatca gattccagcc aacgctcttg cgcatcgacc
     2281 tgaacaaagc ttcggggatg aatcgtccca caactggaag ctcagcgcgg atcaattgtt
     2341 cagtttgaat tttcactccg ttgaagtctt tgaacatctc ctgagcctct gcacgagtgt
     2401 aggcctttac gagaggtcgc gctccgtgtt cagagtattc cacccagcga ctcatgattt
     2461 cttcagggga acgcccgcga aatagttctc cgcggaaaac accgcgatgc agcatcatct
     2521 cgccccagta gttaagcgaa ttcttgtgat agatcatcac cttcgccgtg ccgccaggct
     2581 tgagcacgcg gtgcagttcg caaatcgcgc cagcagtgtc gggcgtgtgg tgtaacactc
     2641 cattagagta gacgacgtcg agggtagcgt cggcgaaggg caaacgttca ccgtcgccga
     2701 ttagaagatt tgttcgttgg ccgtacagcg caaagcgttg ccgcgtaatc gcgattgagc
     2761 gcggcgtcag gtccattccc gtacaaaccg cacccccacg tgcgaactgc atcaagtcgg
     2821 tccccatgcc acatccaact tcgagcaacc gcgctccggc aaactcgttg aaccccatca
     2881 gctcaggcat ccagggagca tattcctggt accttacgcg ctcgatctca tcgaaatatt
     2941 gacgcgttcc gaattcattt tcgcgatcat tttcagcgcc acacggatct tgtccccact
     3001 gctcacgcgc ccgctgtttc tcttccgcta agaaagaatt gctgcgttgc ggttcgggct
     3061 tcgagctaaa catagtgatt tggttgataa gtcccttccc gttcacacca ttgaatctct
     3121 accccggaaa tgctgcaaca ggaacggctt tgccgccgga tgtgagcgcg agcttcgccg
     3181 cgccgctcgg ccaacaagaa acttccacac caagtatcaa gtctctcgcg tcctatcgcg
     3241 ccaagcttgg tacgcgtcga ggatgttctg ggcgttacga gtccaagtat gattggcgac
     3301 cgccgtcgaa cgggccgcag cgcccagagt ttgccgcagg ctgcgggatt caacgaaccg
     3361 tatgatcgct gcactcaatt cctgagcgtt tcccggagac actagcaaag ctgtttcttc
     3421 gtgcttaaga acttcagcaa tttggccgag cctgctggca acgatggcct tagccatagc
     3481 catatattcg aaaagctttg taggagagcc aaaaaactcc gcgcctcctt caagcggcac
     3541 atgcggcgag acgagtacat cacacgcatc cagtagcgcg ggaatgtcgt cgtgctccac
     3601 gctgccatag aagaccactc gaccagtctc ttcctcactc gcaagtagtt tcttcatctc
     3661 gccgtgcaat gctccagtgc ctactatcag gaagcgcacc ggcacattag caggaataag
     3721 tttgattgct tctgctaaca ccaacacgcc atgccagggg ccaaaggtgc cgacaaaccc
     3781 aactagcagc tcttcgcttc gtagaccgag ttccgctcgc ttctcgagtc cgccaacatt
     3841 cggacgaaaa gctttcacat caacgccatt cggattaacg attatcttct ccggcgctat
     3901 accaactcgt tcgagatttt ttcgttccac ttcggagaca acaaaaacac gagccgccgc
     3961 tgccaggttt aatcgttcat aacgctcgag taggtcgagc atgctcacgc gatcccaatg
     4021 ctttcccacc cagacttccg agccattgta ttcaaggaac aacgggcgct gagtgcgcaa
     4081 actcactatg acgcccgccc aactgaagcg tgcgtatcgt tgatagatga agtcgggcgg
     4141 atttggctga acgacggcga gcgccccgct tgtgaacacc tcgttgttgt gcaggtcaaa
     4201 gactgcccgc gtggtgccaa gtggttcggg cctaatcaga gttaacttca cgttgctgtt
     4261 gtcaaaacca gcaagtgcgt cattactcac gagctcaacg ttcgctccca attcaactaa
     4321 tccgccgatg acacctttga tatgactcga ggcaccgcct gcttgtgtac ccggccccgg
     4381 cgttgcgcgc agataggtga ttcttgggtg attggaagtg gacttagttg taactggagc
     4441 aagagttttg cggtgttgga tttcctgttc gagttcgact agttcgcgcc gggagcgcga
     4501 aatgactccg ctactgacaa ttgattcacg cgcaacacga ctcatgccct tgagcaagag
     4561 ggaacctcgg gaatcttgcc gacgcattcc gccatgggca tcaatgagca aaacttcgcg
     4621 cgcgccggct agagcgccga acagcatgaa caagtcttgg cctcgctgcc actccaaccg
     4681 ctccgtcgcg acagcaaaga cttcaggttg atgtcgtcgc aacgcgcgca ggcgtgaagt
     4741 caagttgccg ttttcgattt cgctgcgagg aatcgtttca attgtagctg cggggagaat
     4801 atcactcacg cgctgttggg ctcgtcgcgc atcaccgctc agcacgacca gaatcactct
     4861 catcagagcg tacctctcac actgcgaaag ctcctcagtg tcagcaggcg gcgcaactcg
     4921 cttccgcgca gcaagagtgc gtctgacaac ggccgtcctg tctgcgcagt gaagtaatgt
     4981 gcgacgagaa aaaagatcaa cgcgtaacta atcgttgagg ctattgctgc tcctcgagca
     5041 ccaaagcgcg gcaccagcac aaacaccagg acaatgtttc ctatcagcgt tgcaatccac
     5101 gataagggaa tcgtgcgcgg caaaccaatc gcgtttaagt gttgcaccag gactgattcg
     5161 atgcctacca ggtaaacgcc gggaaggagg atcaagagct gcaccgtcac gtcggcaaat
     5221 gcggcgccgt agagcagcgg caacacataa ctcagcggaa tggcggcgag acaacaaaac
     5281 aagagtaaca acgccgtgtg ccggctcacg agacaggttg tttctccact caaatccggt
     5341 tcggctgcga cgcgcggaaa aaggagtgtg gcaatcacgc ccggcaatag cattagcata
     5401 agtgccacct gcgaagtaac actgtagacg ccggcttccg cggcgccgcg aaagtgattc
     5461 accaccagca agtctgcgcg gaaaatcaaa gcgcccgcga gaatggagat gtgaaacttg
     5521 aatccgtaac ccaacatgcg gcggaagagc ggaaagtcgg ttcgccacgg caaggggttg
     5581 ttcaatctcg cggcgacgaa tcccaccaaa actatcaata gcacgccgat caaaacgctc
     5641 gagatcgtat ttagtgaaac caatacccac agtccggctt gcatcacaat aagtgcgatg
     5701 actgcattga tcagcacaaa cgtctgtccc gccaaatcga ttaggttgaa ctgcgtgatc
     5761 cggcccaccg caagaaaaac attcaggcca atgaatgtta agagttgaaa cggcagcgag
     5821 atagcagcga tggcgaacaa ttttggcgga ataaaaccaa accattctgg tcgcaatttg
     5881 gcgacgccgg tcaaaaccag tgcgagccct gtaccgatca aaagcgcgaa gattagtgag
     5941 ttcattgccg cggcgcggag ttgcagtttg tcttgcgcga tgaagtacgt attagctgag
     6001 ggcagtccaa agctcgcaag ctgcacgaga gtcgcaatcg tgacgttaat caccgcaagt
     6061 tggcctaagc cttcggctcc gagccagcga gccacaatga ttccagcaac cacgctgttg
     6121 ccgaccatca gaacgcgtgt ggcaagtgtc cacgcgacgt gagtggaaaa cttagctgct
     6181 ggagagagag gcggagacgc ggggacacgg tgactcggag atggggggac gcggggacgc
     6241 ggcgatgcgg cgtcagggag agccgctgcc tcgtcatttt ccgacactga aacgtcttta
     6301 ctcatcatta cctcacgcca aactacaacg tccccgcgtt tcctttggcc gt
//
LOCUS       DASUEX010000074         4981 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_590360, whole genome shotgun sequence.
ACCESSION   DASUEX010000074 DASUEX010000000
VERSION     DASUEX010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4981
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_590360"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="VFV61_01270"
     CDS             <1..417
                     /locus_tag="VFV61_01270"
                     /inference="COORDINATES: protein motif:HMM:NF014860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899128.1"
                     /translation="AVEHIAGHKDVRPINLRLVPNCTYCDPEVASVGLTEAKAKEMGY
                     DVRLGKFPFSASGKARILGEEEGFVKIVSEAKYDEILGVHIIGPHATELIAEACVAMQ
                     LESTAEELGRTMHAHPTVSETIMEAAEGVHGLTIHI"
     gene            423..1586
                     /locus_tag="VFV61_01275"
     CDS             423..1586
                     /locus_tag="VFV61_01275"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEX4899129.1"
                     /translation="MDQSSHKTDTKDPRRRRRRPTRSADQSRGPRVFTKYAPRKAADG
                     QELSCTKETTLKPEQLLELYRYLKLTRLVEERLVNLYRQTKVVGGVFRSLGQEATAVG
                     TAYALQPRDFITPLIRDLGAVLVKGIRPREIFAQYMAKAWGPSGGRDLNIHFGDMEKG
                     FLGPISHLGDMIPVMTGVLLGARMKKEPIVAIAYIGDGGMSTGAFHEGLNFAAVQRLP
                     LIVVAEYNHYAYSTPTTLQTAVKDLAEKAASYGIPGHIVDGNDVVACYETTRRAVDFA
                     RRGGGAVLIEAKTYRRKGHAEHDDQRYVPEGEIEWWEKHNDPIDRYERYLLEGSVANK
                     GKLAEITAAVVREIEEDCAWAESSPMPDPEQAAYRVFDNEVVAPALRPEILTG"
     gene            1661..2173
                     /locus_tag="VFV61_01280"
     CDS             1661..2173
                     /locus_tag="VFV61_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899130.1"
                     /translation="MGLVYAEIELTNSDDVALHRRGFLDEARIKRLKLSALVDSRAYM
                     LVINDEIRRQLELPLIQEQVFRLADDTTIKGEVVGPVEIRFENRSTTTRAVVLPGASE
                     VLLGSIPLEDLDVVIDPKRRRLVVNPESPDIAMKPLKQKLVGTILELQFTNPDSKPKI
                     QRPYKRCPQP"
     gene            2158..3186
                     /locus_tag="VFV61_01285"
     CDS             2158..3186
                     /locus_tag="VFV61_01285"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF006667.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEX4899131.1"
                     /translation="MSTAIKKEHKSARPERGGLVTFVEAIRQGIWEEMERDPSVFVIG
                     EDVGAYGGAFKVTDGLLDEFGKGRVVDTPISEGAIVGAACGAALMGMRPVAEFQFIDF
                     ISAGFDLLTNYAAKSRYRWGGGIAAVFRGPCGSGLRTGPFHSLNPESFFLNTAGLKMV
                     EPSTAYDAKGLIKAAIRDPDPVLYFEHKKLYRLPRLREEIPADDYIVEIGKARTVREG
                     RDLTIITFGAMVLTALDAAEELEKEGLDVEIVDLRTLAPLDRTAILDSVKKTSRALIL
                     HEASQTGGIGGEIAALISAEAFEWLDAPVLRLASIDTPVPYSPPLEDYYLPQVKDVVA
                     AARRLAAY"
     gene            3210..3506
                     /locus_tag="VFV61_01290"
     CDS             3210..3506
                     /locus_tag="VFV61_01290"
                     /inference="COORDINATES: protein motif:HMM:NF025392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HEX4899132.1"
                     /translation="MPKYVIEREIPGAGSLSADQLQGVAQTSCNVLHEMGPQIQWVQS
                     YVTGDKVYCVYISPNEEMIREHAQKGGFPANKISEIKTIIDPTTSEAKAQSAGG"
     gene            3594..>4981
                     /gene="sucB"
                     /locus_tag="VFV61_01295"
     CDS             3594..>4981
                     /gene="sucB"
                     /locus_tag="VFV61_01295"
                     /inference="COORDINATES: protein motif:HMM:TIGR02927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase, E2 component,
                     dihydrolipoamide succinyltransferase"
                     /protein_id="HEX4899133.1"
                     /translation="MATEVVMPQMGESIAEGTITRWLKKVGEHVDRDEPLFEISTDKV
                     DAEIPSPAAGTLTEVRFKEGDTVEVNTVVAVLDGAAAGNGSAAPAATTATKPETAPAV
                     ETKPEVAAPEPAPAASTPPAAPAPPAKPAAPPAAVTSSGAVETKAPIAAAQETTAAPA
                     SGTPTVEELRRTKSSPLVRKIAKEHGVDVASLEGTGLSGRVTKNDILSFIESGAPAPA
                     MKSASAPAPARDIPAPPPTKPGEGDRVEQMSVMRKKIADHMVVSRRTSAHVTTVYDID
                     MSAIAKLREQNKDSFLERTGTKLTYMPFIFQAITKGLRKFPIFNAQVSGDQIIYKRDI
                     NLGMAVALDAGLIVPVIKRADDLSISGLARAANDLADRARTKQLKPDEVGGGTFTITN
                     PGVFGGLFGTPIINQPQLAILGVGKIEKRAKVITSPEGDDYIAIRWMSYFALSFDHRV
                     IDGADAERFLAFV"
BASE COUNT         1194 a         1305 c         1452 g         1030 t
ORIGIN      
        1 gccgtagagc acatcgcggg acataaagac gtgcggccca ttaacctgcg cctcgttccc
       61 aattgtactt attgcgatcc ggaagtcgcc tcagtcggat tgacggaagc gaaggcaaaa
      121 gagatgggtt acgacgtgag gctgggcaaa tttcccttct ccgcttcggg taaggcacgg
      181 attctcggcg aagaggaagg cttcgtgaag attgtcagcg aggcgaagta tgatgagatt
      241 ctcggcgtgc acatcattgg cccgcacgct accgagctga tcgccgaggc ttgcgtagcc
      301 atgcaactcg aatcaacggc cgaggaactg ggtcgcacaa tgcatgcgca tccgaccgtt
      361 tcggaaacga ttatggaagc tgccgagggc gtgcacggcc tgacaatcca tatctaagcc
      421 acatggatca atcatcacac aaaaccgaca cgaaggatcc acgccgccgc cggcgccggc
      481 caacccgttc cgcggaccaa agccgcggcc cgcgcgtttt taccaagtat gcgccgcgga
      541 aggccgctga cggtcaggaa ctctcgtgca ctaaagaaac aacgctgaaa cctgaacagt
      601 tgttagagct ttaccgctat ctaaaactga cgcgcctggt tgaggaacgc ctcgtcaatc
      661 tttaccggca gacgaaagtg gtgggcggcg tttttcgttc actcggtcag gaagcaactg
      721 ccgtaggcac tgcctatgcg ctccagccgc gcgactttat tactcctttg attcgcgatc
      781 tgggcgcagt gctggttaag ggaattcggc cgcgcgaaat cttcgcgcag tacatggcga
      841 aggcctgggg accatcaggc ggacgcgatc tcaatattca cttcggtgac atggagaagg
      901 gattcctcgg tccaatttcc catctcggcg acatgattcc cgtgatgacc ggagtgctgc
      961 tcggcgcccg aatgaaaaaa gagccgattg tcgcgattgc ttacattggc gacggcggca
     1021 tgagcacagg agcgtttcat gagggtctga actttgccgc agtgcaacgg cttccgttga
     1081 tcgtagttgc tgaatacaac cattacgctt attccactcc taccaccttg cagacagccg
     1141 taaaagacct ggctgaaaag gccgccagct atggcattcc aggacatatt gtcgatggca
     1201 atgacgtcgt agcctgttat gaaacgacgc gacgcgctgt ggattttgcg cgacgtggcg
     1261 gcggcgcagt tctgatcgag gcgaagactt atcgtcgtaa aggacacgcc gaacatgacg
     1321 atcagcggta tgtcccggag ggtgagatcg agtggtggga gaagcacaac gatccaatcg
     1381 atcgatatga gcgctacctg cttgagggta gtgttgccaa caagggcaag ttggcagaga
     1441 taaccgccgc agtcgtgcgt gaaattgaag aagattgcgc ttgggccgaa agctcgccaa
     1501 tgcccgatcc cgaacaggcg gcctaccggg tgtttgacaa cgaggtagtg gcgccggcct
     1561 tgcggcccga aattctaaca ggctaataat ggtattaccc ttggacaaaa gtcggagttc
     1621 gaaacttcct gaggttgact cacccaacgg agggaaacaa atgggactcg tttatgccga
     1681 aattgaactc acaaatagtg acgacgtagc gcttcatcgg cgaggatttt tagatgaagc
     1741 acgaataaag agattgaaat tgagcgcgtt ggttgatagt agggcttaca tgctggttat
     1801 caatgacgag attcggcgac aactggagtt gcctttgatt caggagcaag tcttccgctt
     1861 agctgacgat accactataa aaggcgaggt ggtcggcccg gttgagatac gctttgagaa
     1921 tcgttctact acaaccagag cggttgtttt accgggcgcc agtgaggtat tgctcggctc
     1981 tattcccctg gaggatcttg acgttgtgat tgatcccaag cggcggcggc tggtagtgaa
     2041 tcctgagagc ccggatatcg cgatgaaacc cctgaaacaa aaactcgttg gaacaattct
     2101 agagcttcaa ttcacaaacc cagattccaa gcccaaaatc caaagaccat ataagcgatg
     2161 tccacagcca taaaaaaaga gcacaagtcg gcacggcctg agcggggtgg gttggttact
     2221 ttcgtggaag ccatccgtca gggcatctgg gaagagatgg aacgcgaccc ttcggttttc
     2281 gtcattggcg aagacgtagg ggcttacggc ggagctttca aggttactga cggcttgctg
     2341 gatgagtttg gcaaaggacg cgtcgttgat actcccatct cggaaggcgc catcgtggga
     2401 gctgcctgtg gtgcggccct gatgggcatg agaccggtgg cggagtttca gttcatcgac
     2461 ttcatttccg caggctttga tctgctgacg aactacgcgg ctaagtcgcg ttatcgctgg
     2521 ggcgggggaa tcgcggcggt attccgcgga ccctgcggct cgggattgcg caccggacca
     2581 tttcactctc tgaatccgga aagtttcttt ttgaataccg cgggattgaa gatggtcgaa
     2641 ccatcgactg cctacgatgc gaaaggtttg atcaaggccg cgattcgcga cccggatccg
     2701 gtgctctatt ttgaacataa aaaactttac cgcctgccgc gcctgcgcga ggagattccc
     2761 gcggacgatt acattgtgga gatcgggaag gcccgtactg tgcgggaggg ccgggatctg
     2821 acaatcataa cttttggcgc gatggtattg accgcacttg atgccgctga agaactcgag
     2881 aaggaagggc ttgatgttga gatcgtcgat ctgcgcacac tcgctcccct ggatcggacg
     2941 gcaatcctgg atagtgtcaa gaagaccagt cgggcgctga ttctgcacga ggcctcgcag
     3001 accggaggca tcggcggcga gattgcggcg ttgattagtg ctgaagcttt tgaatggctc
     3061 gatgcgcctg tattgcgcct ggcttccatt gatacgccgg ttccttactc accgccgctc
     3121 gaagattatt acttgccgca agtgaaggac gtggttgctg ccgcacgaag actggcagcc
     3181 tattgattca gcaaactgag gaggaaacga tgccgaaata cgtgattgag agagaaattc
     3241 ctggcgccgg cagtctttca gcagatcaac tgcaaggtgt ggcgcagact tcctgcaacg
     3301 ttttgcatga gatgggcccg cagatccaat gggttcagag ctatgtgacc ggcgacaaag
     3361 tctattgtgt ttacatttcg ccgaacgaag agatgattcg cgagcacgcg caaaaaggcg
     3421 gtttcccggc caataaaatt tcagaaatca agacgataat cgatccgact acttctgaag
     3481 caaaagctca atccgccgga ggctaaaact gtcttgacac gtctgcagga aggcgagtgc
     3541 aagccgcact tccacactgc aaatgcatta aagttgttca aagtggagca atcatggcaa
     3601 ctgaagtagt aatgccgcag atgggtgagt ccattgcgga aggaaccatc accaggtggc
     3661 tgaagaaggt tggcgagcac gttgatcgtg acgagccgct gtttgaaatc tcgactgata
     3721 aagttgacgc cgagattcct tcaccagcag ccggcacgct cacggaagtt cgtttcaagg
     3781 aaggcgacac cgtggaagtg aatacagtcg tggctgtgct tgacggtgct gcggccggca
     3841 acggatctgc tgcgcccgca gccaccacgg ccacgaaacc ggaaacagcg ccagcggtgg
     3901 aaacgaaacc tgaagttgct gctcctgagc ctgcgccggc tgcttcaact ccgccggctg
     3961 caccagctcc gcctgcaaag ccagcggcgc cgcccgcggc cgtgacgtca tccggtgcag
     4021 tagagacgaa agctccaatc gcggcagcgc aggaaactac cgctgcgcca gccagcggca
     4081 cgccaaccgt cgaagaactg cggcgcacaa aatcaagccc actcgtgcgc aagattgcga
     4141 aggagcatgg tgtcgacgtt gcgagtctgg aaggaacagg actaagtgga cgcgtaacca
     4201 aaaacgacat cctcagtttc atcgagtcgg gagcaccagc accagcaatg aagagcgcct
     4261 cggcaccggc gccggcgcga gacattccag cgccgccgcc cacaaaaccg ggagaaggcg
     4321 atcgcgtcga gcagatgagt gtgatgcgca agaagatcgc cgaccacatg gtggtttcgc
     4381 gacgtacttc ggcccacgtg acgacggtct acgacatcga catgtcggcc atcgccaaat
     4441 tgcgggagca gaacaaagac tcgttcctgg agcggaccgg caccaagctt acgtacatgc
     4501 cgtttatctt ccaggctata acgaagggcc tgcgaaagtt cccgatcttt aacgcccagg
     4561 tgagcggcga tcaaatcatc tacaaacgtg atatcaatct cgggatggcg gtcgcgcttg
     4621 acgcgggttt gattgtcccg gttatcaaac gcgccgacga cctttcgatc tccggactgg
     4681 cccgcgcggc caatgatcta gcggaccggg cacgcacgaa acaattgaaa ccagacgagg
     4741 ttggcggcgg gacctttacg attacgaatc ctggcgtttt tggtggtcta tttgggacgc
     4801 caattatcaa tcaaccgcaa ctggccatcc tcggcgtcgg caagatcgaa aaacgcgcca
     4861 aagtgatcac ctcaccggaa ggcgacgatt acatcgcgat ccgttggatg tcttattttg
     4921 cactcagctt tgatcatcgt gtgattgacg gcgcggatgc cgagcgcttt ttggcgttcg
     4981 t
//
LOCUS       DASUEX010000075         3041 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1385096, whole genome shotgun sequence.
ACCESSION   DASUEX010000075 DASUEX010000000
VERSION     DASUEX010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1385096"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..911
                     /locus_tag="VFV61_01300"
     CDS             <1..911
                     /locus_tag="VFV61_01300"
                     /inference="COORDINATES: protein motif:HMM:NF015413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX4899134.1"
                     /translation="IEVAGAWRVTGADVSLQGLWRRLAPAGFMGTRKPVEVIDWSDVG
                     YLATDEATVQLKSASGKLARLHPVEIARLAEALSYHHGSEVVESLDDETAAETLEEMA
                     AAQQARIISDMDEERAADILEWMSPDEAADVLGDLPEEKAEELLGLMEGEEQADVAEL
                     LPYEDDTAGGLMTTEFVTLPRELTVGAALARLREMAETPNMIYYLYVVEAEDSWQLIG
                     VIALRSLILADQTAPLAAVMRTEFQSAHPNEPAKDVARRIAEYNLLALPVVDQAGDIL
                     GIITVDDAMEILLPKNWRQRLPRLFA"
     gene            967..2283
                     /locus_tag="VFV61_01305"
     CDS             967..2283
                     /locus_tag="VFV61_01305"
                     /inference="COORDINATES: protein motif:HMM:NF037982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEX4899135.1"
                     /translation="MLERRAAVRAAGRRLLRRGARGVRNVTPRQRLLAYLAILGPGMI
                     TANAGNDAGGIATFASVGADHGYQLLWILIPITISLGLVQEMCARMGAATGKGLADLI
                     RERFGVRWTALVMLALLIANGGVTVSEFVGIAAATELFGIPRFISVPLAAVSIWWLVV
                     KGSYQRVERVFLLMALVFLGYIASAFLAQPDWTKVAAGLVRPSFSFDSAYLFTLVAVV
                     GTTISPYMQVYIQSSVVEKGVTIENYRRTRIDVWVGTVFAVLIVFFIVISTAATLHRK
                     GIQVASAADAARALQPLAGNYAEALFAVGLLGASMLAAGVLPLATAYSVSEALGFEKG
                     VSRSFREAPIFLGIFTFLVAVGAAIAVLPNLPLIKVLLVTQVINGLLLPVVLFAVLRL
                     INDKELMGAQVNGPINNIAAWLTTIVVTLLSLLLILVTIFPRLLSF"
     gene            2397..>3041
                     /locus_tag="VFV61_01310"
     CDS             2397..>3041
                     /locus_tag="VFV61_01310"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEX4899136.1"
                     /translation="MIRSGSNNRRILFVVTAAIVLLLLYRVVSFDTQIHQLLAARMGG
                     LAARAVTVVNVIRLVLLGILTYLFVRVLNSLIFGLIYRMRSGYEAPTLVRNIFSIVAF
                     TALFLLIFSAIFEGVNLGALFTTSAIFGVILGLALQDTLGNFFAGISLQADRPFQIGD
                     VITVGAQRHSGVVEEITWRAVKIRTFSNHVVLISNSTAAREPIEVCPRDNLNARH"
BASE COUNT          578 a          854 c          870 g          739 t
ORIGIN      
        1 tgatagaggt tgccggcgcg tggcgtgtca ccggcgccga tgtcagtttg cagggtcttt
       61 ggcggcgtct cgctcccgct ggcttcatgg gcactcgcaa accagtggaa gtaattgact
      121 ggtctgatgt gggttacttg gcgactgatg aagcgacagt gcaattgaag tccgcgagcg
      181 gcaaactcgc acgacttcat cccgtagaaa ttgcgcgact cgcggaagcg ctttcttatc
      241 accacgggtc ggaagttgtt gagtcgctcg acgatgaaac cgccgcggaa acgctcgagg
      301 aaatggccgc cgcgcagcag gcacgcatca tcagcgacat ggatgaggag cgcgccgcgg
      361 acatcctgga atggatgtca cctgacgaag ctgctgacgt actcggcgac cttccagagg
      421 aaaaagctga agagttactg ggtctgatgg aaggcgaaga gcaggctgac gtcgctgagc
      481 tgttgcctta tgaggacgat actgcgggcg gattgatgac gacagagttt gtcacgttgc
      541 cccgcgagct caccgttggc gccgccctgg cccgcctgcg tgaaatggcc gagacgccaa
      601 acatgatcta ttacctctac gttgtcgaag ccgaagattc atggcagcta atcggggtga
      661 ttgctttacg gagtctgatt ctggctgatc agacggcccc gctcgcagcg gtgatgcgca
      721 ccgagtttca gtcggcccac ccgaacgaac cggcgaagga cgtggcccgc cgaatcgctg
      781 aatacaatct cctcgcttta ccggtcgtag atcaagcggg agacattttg ggaattatca
      841 ccgtagacga tgcgatggaa atactgctac cgaagaactg gcggcaaaga ttgccgcgac
      901 tctttgcctg agacatcgtg cacaacggac gcgagttgtt cgttatgaac gacggagaac
      961 ctccaagtgt tggaacgaag agcagcggtg agggccgcgg ggcgacgctt gttgcggcgc
     1021 ggcgctcgcg gtgtgcgcaa cgttactcct cgccaacgtt tgcttgccta cctggccatt
     1081 ctcggtccgg gcatgatcac ggccaacgct ggtaacgacg ccggcggtat cgcgaccttc
     1141 gcgtccgttg gcgccgatca cggttaccaa cttctttgga tcctaatccc tatcactatc
     1201 agtctcggtc tggtccagga gatgtgcgcc cgaatgggag cggcaactgg caaaggcctc
     1261 gccgatttga ttcgcgagcg gttcggcgtg cgctggaccg ccctggtgat gctcgctctt
     1321 ctaattgcca atgggggcgt gacggtttcg gagttcgtgg gcatcgccgc agcgaccgaa
     1381 ttgtttggca tccccagatt catatcagtg cccctagcag cggtcagcat ctggtggctg
     1441 gtcgttaagg gctcttacca gcgcgtggag cgtgtgtttc tgctcatggc gctggtcttc
     1501 cttggttaca tcgcctcagc ctttcttgcg cagccggact ggacgaaggt ggcggcagga
     1561 ttggtacgac cgagttttag ttttgacagc gcttatctgt ttactctcgt ggccgtcgtc
     1621 ggcacgacaa tctcgcccta tatgcaggtc tatattcaat catcagtcgt agagaagggg
     1681 gtgactattg aaaactaccg gcggacgcgc attgatgttt gggtgggaac cgtcttcgct
     1741 gtgctgattg tttttttcat cgtgatttct accgccgcga cgctccatcg aaaaggcatt
     1801 caagtagcca gcgccgctga cgccgctcgg gctctgcaac cccttgccgg taactacgcc
     1861 gaggcgctct tcgcggtcgg tcttttaggc gcatcgatgt tggcggcggg cgtgttgccc
     1921 ttagcaaccg catactccgt cagcgaagcc ttgggctttg aaaaaggcgt ttcgcggagt
     1981 ttccgcgaag ctcccatttt cctggggatt tttacctttc tggtggcggt gggggcggcg
     2041 attgcagtgc ttcccaattt gccactgatt aaggtgttac tcgtcaccca agtcataaac
     2101 ggattgctgc tgccggtagt cctttttgcc gtcttgcgcc tcattaatga taaggaactg
     2161 atgggcgcgc aggtcaacgg gcctatcaac aatattgctg cctggctaac cacgatcgtg
     2221 gttaccctac tttcattact tttgatcctg gttactatct ttccccgcct cctgtccttc
     2281 tagttgctca cgcgcccgtt tatttctcct cgccaagctt aacaatcggt gataaaagag
     2341 agccgttagc tgcggtccaa atctgcaaac cttgcccgcg aaattccagt agcaaagtga
     2401 ttcgctcagg ctccaacaac aggcgcatcc tcttcgtagt cactgccgcg atcgtgctgc
     2461 tgttgctcta tcgtgtcgtt tccttcgata cccaaatcca tcagctgctg gccgcgcgga
     2521 tgggcggcct cgcagcgcgc gccgtcacgg tcgtcaacgt catacggttg gtgctgctgg
     2581 gcattctcac ctacctgttc gtgcgcgtac tcaacagcct catctttggc ttgatctacc
     2641 gcatgcgcag cggctacgaa gcgccgacgc tggttcgcaa tatcttttcg atagtcgctt
     2701 tcacagcgtt gttcctgctg attttttcgg caatttttga aggcgtcaat ctaggcgctc
     2761 tcttcaccac ttcagccatc ttcggcgtta ttctcggtct tgcgcttcag gatactttgg
     2821 gaaacttttt tgccgggatc tcgctgcagg cggatcgacc tttccagatt ggcgatgtca
     2881 ttacagtcgg cgcccaacgg cactcaggcg tggtcgaaga gatcacctgg cgcgccgtta
     2941 agatacgcac tttctcgaat cacgtggttc tgatcagcaa cagcaccgcg gcacgcgaac
     3001 ccatcgaagt ctgcccgcgc gacaatttga acgcgcgaca t
//
LOCUS       DASUEX010000076         3144 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1048648, whole genome shotgun sequence.
ACCESSION   DASUEX010000076 DASUEX010000000
VERSION     DASUEX010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3144
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1048648"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="VFV61_01315"
     CDS             complement(<1..180)
                     /locus_tag="VFV61_01315"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:NF013575.4"
                     /note="mediates pseudouridylation (positions 38, 39, 40)
                     at the tRNA anticodon region which contributes to the
                     structural stability; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEX4899137.1"
                     /translation="MNFKLSLQYDGTDFHGWQIQEGLRTVQGELTAALSLVDDREVVV
                     HGSGRTDAGVHAEGQV"
     gene            complement(318..890)
                     /gene="pyrE"
                     /locus_tag="VFV61_01320"
     CDS             complement(318..890)
                     /gene="pyrE"
                     /locus_tag="VFV61_01320"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEX4899138.1"
                     /translation="MDTAAVIERFKATGALLEGHFVLTSGLHSPNYLQCALLLQHPRE
                     AEEFAGALAGHFQNDEIQLVAAPAIGGIVIGYEVARQLGARFIWTERVEGKMTIRRGF
                     SVKPGERTLVVEDVITTGGSTLETVETLREAGANVIGAASIIDRSSGRADVGVARVAL
                     ATLDVPAVDPAFCDLCRQGIPVVKPGSRKE"
     gene            932..1813
                     /locus_tag="VFV61_01325"
     CDS             932..1813
                     /locus_tag="VFV61_01325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HEX4899139.1"
                     /translation="MPPFTNLISDSTLVQETIELLRDCGGRANAAEIVDAVFKVSHID
                     EELAGLLVADLVKGDRRFKIIDSKTIALQEDDVETRLLNELEFVVVDLEATGAKMPPN
                     RVIELGAYRIRNGRIVDNFLTLVNPEIPIPRFVISLTGITNEMVKSAPLFADVAPRWL
                     DFVSDAVLIAHNAPFDTSFLNHEISRVYPGHRMVNSHLCTVRLSRRVFPGLNNYRLDT
                     VADHFSIPISHRHRAGSDALATAEVFLHLLSRFEDHGVTDLATARRFQFFEPPDREAK
                     PRDTEAVAPLLPLVPGF"
     gene            complement(1796..2389)
                     /locus_tag="VFV61_01330"
     CDS             complement(1796..2389)
                     /locus_tag="VFV61_01330"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEX4899140.1"
                     /translation="MLVTFGPFGLFVVALLDSSFVPLPSSADALMLLLTAAHPRWMLI
                     YAAVATAGSAVGCLILYYVSRRAGTRALARFSEKKQKRVKDWIDRYDVISVLVASLLP
                     PPFPFKLFVVSAGVFRFSVTRFLIAIIVGRGFRFLLEGYFAIRYGAQAKAVLAQYYPW
                     IGLGLAVFVVAVFVTRAMLKRRSGKETKVEEGLETGN"
     gene            complement(2482..3105)
                     /locus_tag="VFV61_01335"
     CDS             complement(2482..3105)
                     /locus_tag="VFV61_01335"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEX4899141.1"
                     /translation="MFDWLSAIGRWLEQYVISVPLYIAAPAMVLIGALDSSLLSLPEI
                     NDYLVVGRCYKYPSAAFYFPLFAALGSVLGCLLLYTIMRRGGQALLRKRFKPESIQRV
                     ERAYARFGVFAIAIPAILPPPLPFKIFVATAGTLEYPRWKFLLTVMIARSVRYYVEGV
                     LAVFYGRRVLLFMKDNGLVILSIVATLAVIAVLVYYLINRRRMARAL"
BASE COUNT          770 a          895 c          819 g          660 t
ORIGIN      
        1 gacctggcct tcggcatgga ctccggcatc agtgcgccct gatccatgaa ccacgacttc
       61 acggtcatca accaatgaca acgcggcggt gagctcgcct tgcacggtcc gcagtccttc
      121 ctggatctgc cagccatgaa aatcggtgcc gtcgtattga agggagagct tgaaattcat
      181 tcaactgaat ttgaacgagt ctttgtgctt agtactttgt gctttgtact ttgttcgcga
      241 ccgttactgg aatggcttta gccactaatg agtcaagatt gaatggcgaa caaagttcaa
      301 agcactaagt tcaaagttca ttcctttctg ctgccaggtt tgaccaccgg aattccctgt
      361 cgacaaagat cacaaaacgc tggatcgacc gccggcacat ccaaagtcgc cagcgccact
      421 cgcgccacac ccacatcagc ccgacctgat gaacgatcaa tgattgaagc ggcgccgatg
      481 acattggcgc ctgcctcccg taatgtctcg acagtttcca gcgttgaacc accggtagta
      541 atcacgtctt caaccaccag ggtgcgttcg ccgggcttca cagaaaatcc acgccgaatt
      601 gtcatcttgc cttcgacgcg ttcggtccag atgaagcgtg caccgagctg tcgtgccact
      661 tcatagccaa tcacaattcc accgatggcc ggcgccgcca ccagttgaat ctcgtcgttt
      721 tgaaaatgcc cggcgagcgc gccggcaaat tcttcagctt cccgggggtg ctggagcagg
      781 agggcacatt ggaggtagtt tgggctgtgc aaaccgctcg tcaacacaaa gtggccctct
      841 agcagtgccc ctgtcgcttt gaatcgttcg attactgctg ctgtatccaa gtcgtacctc
      901 gaaaactaat gcgtgttaca atcgccaccc gatgccgccg tttaccaacc tgatttcaga
      961 ttctactttg gtccaggaaa caatcgaact gctccgcgat tgtggtggcc gcgccaacgc
     1021 tgccgaaatc gtggacgccg tcttcaaagt ttctcatatc gatgaagaat tggcgggttt
     1081 gctggttgcc gatttggtaa agggcgatcg tcgtttcaaa ataattgaca gcaagacgat
     1141 agcgttgcag gaagacgacg tcgagactcg cttactaaac gagctggagt ttgtcgttgt
     1201 tgatttggaa gcgactggag ccaagatgcc cccgaatcgc gtcatcgaac tgggcgccta
     1261 ccgcatccgc aacggccgca tcgtcgataa ctttctcacg ctcgttaatc ccgagattcc
     1321 cattccgcgg ttcgtgatta gtcttaccgg aatcaccaac gaaatggtca agagtgctcc
     1381 gctgttcgct gacgtcgcgc ctcgctggct tgatttcgtc agcgatgccg tgcttatcgc
     1441 ccataatgcg ccgtttgata cgagctttct gaatcatgag atttcgcgcg tctatcccgg
     1501 ccatcgcatg gtcaattcgc acctgtgcac tgtaaggctt tcgcgccggg tgtttcccgg
     1561 cctcaataac taccgcctcg atacagttgc agatcatttc tcgataccga tttcccatcg
     1621 ccatcgtgct ggcagcgacg ccctcgcaac cgcagaagtt ttcttgcatc tgctctcgcg
     1681 cttcgaagac cacggcgtga ccgatctggc aacggcccgc cggtttcaat tcttcgagcc
     1741 tccggatcga gaagcgaaac cgagggacac ggaagcggtg gcgccattgc taccgctagt
     1801 tcccggtttc taatccctcc tccacctttg tttcctttcc tgatcggcgc ttcaacatcg
     1861 cccgcgtcac gaatacagcc acgacaaaga cggccaaccc gagaccgatc cagggatagt
     1921 attgagccag gaccgccttc gcctgggcac cgtaacgaat cgcaaagtaa ccttccagca
     1981 gaaagcgaaa gccacgtccc acgatgatgg caatcaagaa tcgggtaacg ctgaaacgaa
     2041 agacgccggc cgagacgacg aagagtttga agggaaaagg tggcggcaac agtgaggcga
     2101 ccaggacaga aatcacatcg tagcgatcga tccaatcctt cacccgtttc tgtttctttt
     2161 ccgagaagcg cgccagggcc cgtgtgccgg cacgccgcga gacgtaatag aggatcaaac
     2221 aaccgacggc tgagcccgca gtggcaacgg ctgcataaat caacatccaa cggggatgcg
     2281 cagcggtcag cagtaacatc agcgcatcgg cgctgctcgg caaaggcaca aagctcgaat
     2341 cgaggagcgc aacaacaaac aatccgaacg ggccgaaggt tacaagcact tcggacaaac
     2401 ggttgaggct ttttgaaaag ggatgaagca cggataggag tcggatgtcg gatgtcggat
     2461 gtcagatgtc agccagtcgc ctcataatgc ccgcgccatc cttcggcgat taatcaggta
     2521 ataaacaaga accgcgatta ctgccaatgt ggcaacgatg ctcaggatta ccaacccatt
     2581 gtctttcata aacaatagca cgcgccgtcc ataaaagact gccagcaccc cttcaacgta
     2641 gtagcgcacg gagcgcgcga tcatcaccgt caagagaaac ttccagcgtg gatactcaag
     2701 cgtgccggcg gtggcgacaa atattttgaa aggcaaaggg ggcggcagga tcgcgggaat
     2761 ggcaatcgca aacacgccaa agcgcgcata agcacgttcg acgcgctgaa tactttccgg
     2821 cttgaaacgc ttgcggagta aggcttggcc accgcgccgc atgatcgtgt agagcagcag
     2881 gcagccgagc acggatccga gggcggcaaa aagaggaaaa taaaaagcag cgctgggata
     2941 cttgtagcac cgaccgacta cgaggtagtc gttgatctcg ggcaacgaca gcaaggatga
     3001 gtcaagcgcg ccaatcaaca ccatcgccgg agcggcaata tagagtggga cgctgatcac
     3061 atactgttcg agccagcgtc cgattgcgct taaccagtca aacattcgtc acaagttaca
     3121 gtcctgagtc ttaagtctca agtc
//
LOCUS       DASUEX010000077         2585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_237425, whole genome shotgun sequence.
ACCESSION   DASUEX010000077 DASUEX010000000
VERSION     DASUEX010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237425"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..551)
                     /locus_tag="VFV61_01340"
     CDS             complement(<1..551)
                     /locus_tag="VFV61_01340"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX4899142.1"
                     /translation="MPEIAAFIMSSTTRNRVLALVLGLAVISQSLIFSLGLALLPQDI
                     AGGISAFRGQDIMGGAAIVFKRPQRLRDIVGGASMLVVKRQARPTRTTEVSRTTEVAR
                     ATPPRDRRRPPPVVTPPRVTEEDKGEAFKNQGNTYYDLGQFAQAIEAYQNALKHTPKD
                     PFIYNNLGAAYFSVGRNKEATDA"
     gene            complement(544..1980)
                     /locus_tag="VFV61_01345"
     CDS             complement(544..1980)
                     /locus_tag="VFV61_01345"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX4899143.1"
                     /translation="MNRGERASAAGSDLSSEKRESAAAARVAKLLNQFLFVCLLGLIF
                     AAAIPYGTVEAWWKAAFVCFVFVLWIMGTIENLLNPRSRALDKQLFMPLFALVGFALL
                     QSVPLGTRSHPAIPYQIWNAISADPYQTQFFAAQLLAIVLAGAMFTRYASTDRRVRAL
                     VNVIIAVAVASAIFGILRQTNQHGMGFGLPLLSPGRGYGQFINPNHFALLMEMAFGLV
                     LGMILGGNRKRERLLVYGAALLVVWTALVLANSRGGLLSMLAQIVAAVLLYTVVVPAS
                     KTTGRRRKILAFLRSLRARILLLAVLLVGVVIGTLWIGGDRLASRFEDLSGGVSPAET
                     RTGGSRNEIWRATWHMFKANPISGVGLGAYWAAITEYHDASGSLTPQEAHNDYLELLA
                     SGGIVGAGLGCWFLVVLGKRIKANLRSANSFRRGVRYGAILGVIGVLIHSLFDFGLHM
                     LVNALVFAALVVIATQSLTEHRSRRSNA"
     gene            complement(2107..>2585)
                     /locus_tag="VFV61_01350"
     CDS             complement(2107..>2585)
                     /locus_tag="VFV61_01350"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX4899144.1"
                     /translation="LMMDYHRQNRVDTRIARIFNTYGPRMLENDGRVVSNFVVQALRG
                     EPLTLYGAGDQTRSFCYVDDLVEGLIRLMNSDALHEPVNLGNPIEFTIKELAAEVITA
                     CASKSDLKYLPLPEDDPRQRKPNIERAQTRLGWNPTIQLRQGLEKTVAYFSSRLKQ"
BASE COUNT          618 a          753 c          683 g          531 t
ORIGIN      
        1 aaggcgtccg tggcttcctt gtttctgccc acgctgaaat acgctgctcc caggttgtta
       61 tagatgaacg gatccttcgg cgtgtgcttt aaggcgttct gataggcttc gatcgcttgc
      121 gcaaactggc ccaggtcata gtaggtgttg ccctgattct taaacgcttc gcccttgtcc
      181 tcctcagtaa cccttggcgg cgttactacc ggaggtggcc gtcgacgatc tcgaggaggc
      241 gtcgctctgg caacttccgt cgttcgagaa acctccgtcg tcctcgttgg ccgtgcttgc
      301 cgtttcacaa ccagcatgga agcgccaccg acgatgtcgc gcaggcgttg cggccgcttg
      361 aagacgatgg cagccccgcc catgatgtct tgaccgcgaa aggcagaaat accgccggcg
      421 atgtcctgag gcaaaagcgc caaccccaaa ctgaagatca gcgactgaga aatcacagcc
      481 aatcccagca ccagggcgag gacgcggttg cgtgttgtcg aactcatgat gaaagcagcg
      541 atctcaggca ttgcttctcc gggacctgtg ttctgtcaga ctctgggtgg cgatcacaac
      601 gagcgccgca aatactaacg cgttgacgag catgtgtaac ccgaaatcaa aaagactgtg
      661 aatcagcacc ccgatgacac ccagaatcgc cccataacgg acgccacggc gaaacgaatt
      721 tgccgaacgc agattggctt tgattcgctt ccccaaaacg accagaaacc agcaacccaa
      781 tccagcgccg acgattcctc cactcgccag tagctccaaa taatcgttgt gggcttcctg
      841 tggagtcaga ctgccggagg catcgtgata ctccgttatg gccgcccaat aagcgcccag
      901 gcctacgccg gagatggggt tcgccttgaa catgtgccag gtcgcacgcc agatttcatt
      961 ccggcttcct ccggttcttg tttcggcggg gctcacgcct ccacttaggt cttcaaaccg
     1021 gctcgccagt ctatccccgc caatccaaag tgtgcctatc acgacaccca caagaagaac
     1081 ggcgagtagc agaatccttg cccgcagcga tctcaaaaat gccaggatct ttctccgcct
     1141 gccagttgtc tttgatgcgg gcaccacgac tgtgtatagc aaaactgcgg cgacaatttg
     1201 ggccaacatc gagagcagcc cgccgcgaga attagcaagc accagcgccg tccaaaccac
     1261 caggagcgcg gcaccataaa ccagcagccg ttctcttttc cgattgccgc cgaggatcat
     1321 gccgagaact aatccaaacg ccatctccat gagcaacgca aagtggttgg gatttatgaa
     1381 ctggccatag ccgcgacccg gactcaggag gggtaagccg aatcccatcc cgtgctggtt
     1441 ggtctgacgc aatatgccaa agatggcgct ggcaacagct accgcgataa tcacgttaac
     1501 gagcgccctg actcgacgat ccgtcgatgc gtagcgagtg aacattgcgc cggcgaggac
     1561 gatcgccagt agttgcgcgg caaaaaactg tgtttgataa ggatctgcgc tgatggcatt
     1621 ccaaatctgg tacgggatcg cggggtgcga acgagtccct aggggaacac tctgaagcag
     1681 tgcaaaacct acgagcgcaa acaacggcat gaacaattgt ttgtccagag ctcggcttct
     1741 cggattgaga agattctcga tcgtccccat aatccaaagc acaaacacga aacagacaaa
     1801 cgccgccttc caccaggcct caactgtgcc gtaggggatc gcggctgcga agatcaatcc
     1861 caacaggcaa acaaacagga attgattcag aagctttgct acgcgagccg cagcggcact
     1921 ctctcgcttt tcggacgaca ggtccgagcc tgcggcggaa gcgcgctcac ctctgttcat
     1981 ttgatcagcc ttgccggccc gatatctaag tgcaggatgc atgcgcactc gcaaccaaga
     2041 aatgagatat tgaagattac ccggtttgca ggaaacagta aggcggtaaa acgatgtggc
     2101 gacgccttat tgttttagtc gggagctaaa gtaggcaacg gtcttttcca aaccctgtcg
     2161 caactgaatg gtcggattcc agccgagccg ggtttgcgct cgttcgatgt tgggcttgcg
     2221 ctggcgcgga tcgtcttcag gcagcggcag atacttcaag tccgactttg aagcacacgc
     2281 agtaatcacc tcagccgcta gctctttgat cgtgaactcg atcgggttgc ccagattgac
     2341 tggttcatgc agcgcgtcgg aattcattaa cctgatcaaa ccttcaacga gatcgtcgac
     2401 gtaacaaaaa gagcgcgtct gatccccagc accatacaag gtcaacggct caccgcgtaa
     2461 cgcttgtacg acgaaatttg agacgacccg gccatcgttc tcgagcatgc gcggaccata
     2521 ggtattgaag atgcgcgcga ttcgcgtgtc gactcgattc tgccggtgat agtccatcat
     2581 caaag
//
LOCUS       DASUEX010000078         2397 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_132627, whole genome shotgun sequence.
ACCESSION   DASUEX010000078 DASUEX010000000
VERSION     DASUEX010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132627"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            419..>2397
                     /locus_tag="VFV61_01355"
     CDS             419..>2397
                     /locus_tag="VFV61_01355"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HEX4899145.1"
                     /translation="MTRTSFKVSRLLPRFLAAVSLALTLSGLVTTTQAQQPAAAPAGT
                     RYDITNYRIEAQLIPEQHLLRAGADITVLPLEPTRSLVFELNGSLKVESVETGGKVLS
                     GMVQDAVGVGALGPNVRVDLGQVMPANQPITLRFRWSGALMSPEGGPLATRRLAYVGN
                     EGSYLMYASRWFPFHDYAVDRATADITLVVPTGTQVAGTSDDPVVGIPSAKDGTTRYR
                     FVQKRPELIGNFVAGQYISKTLHFGNYDILFYAKPGSENRINSYAELMGHVLEFYTKQ
                     YGKPLFGNRLVVAQVDDDTLDTYSGTGMIFLAGRMFDSTRPVPEEKLQREVAYQWWGQ
                     TVGLKSFDDAWISQGLAEWSAFAFRESTLTGGALDAAQREQQERALTFEQTASIARAP
                     GALDDQSAAYQSIVFHKGSMVFRMLRETIGTEKFDQLLRNFLETYRGKSASIDDFEKL
                     TTQVNGSNMRYFFAQWVEGTGVPEFTVDYQIIRTRAGKFRTRGTVKHTLQTLRMPVQL
                     MLRSEGDSQTTTTTIEERSEDFDFESNGQPLEVVVDPNYRILRMSDELRVSIIARRGI
                     EQMKEGLYAEAQQQFEAALKLDRSNSWVYYNLGLLFLEQRNWGQALDNFDAALNGTLK
                     PSWIEVWARIKRGNAYDAQGERSRAVSEYNKAVQSG"
BASE COUNT          545 a          643 c          695 g          514 t
ORIGIN      
        1 aattgccgat ttaaggaatg atccggcctg gctttgcaca aagggcctga cgcgggtcga
       61 aagaactgat gtgggtcgag agaactgatg tggatcgaaa gaattagaat caaacgcagt
      121 agcgaacgat ccgtgtacac ccaagcgaaa ggccgccaat tggcaatcgg caattgggca
      181 atcgccaatt cctgcaatcg ccaatcttcc cggccaaccg cctacgcctc cgcttacatc
      241 caaaagcccg actgcataca cagcccgact gctatagagg gcggttttac cccgtatcct
      301 tttccgaggc cttcgagcgc gtgttacgga acccgggcga gccggccagg tcgctactcg
      361 gatagacttc gcggactgta caatcgccag tgttttctcg atgattagca ggaaaatcat
      421 gactcgaaca agcttcaaag tttcccgact cctcccccgc tttcttgccg ctgtatccct
      481 cgcgctgacg ctgtccggct tggtcactac gacgcaggca cagcaaccag ccgcggcgcc
      541 ggccggaacc cgttatgaca ttacgaacta tcgcatcgaa gcccagttga ttcccgagca
      601 acatctgctg cgtgcaggtg ccgacatcac cgttctgccg ctggagccca cgcgttccct
      661 cgtgtttgaa cttaacggct cgctcaaagt tgaaagcgtt gaaaccggtg gtaaggtcct
      721 cagcggtatg gttcaggatg ccgttggtgt cggcgcgctg ggtccgaacg tgcgggtaga
      781 tctgggtcag gtaatgccgg ctaatcagcc gatcaccttg aggtttcgct ggagcggggc
      841 tttgatgtcg cccgaaggtg gcccgctggc gacgaggcgt ttggcttacg taggaaacga
      901 aggttcgtat ctgatgtacg cctcgcgttg gtttcctttc cacgactatg ccgtggatcg
      961 cgcgactgcc gacatcactt tggttgtgcc cactggcact caggtggcag gtacgagtga
     1021 cgatccggtt gtcgggattc cgtctgcgaa agacggcacc acgcggtatc gctttgtgca
     1081 aaagcgaccg gagctaattg gaaactttgt cgccgggcaa tacatcagca agacacttca
     1141 cttcggcaac tacgacattc tcttttatgc caaaccgggt agcgagaatc gcattaatag
     1201 ttatgcggag ctaatgggtc acgtgctcga gttttacacg aagcaatacg gcaaacctct
     1261 ctttggtaat cgtctggtag tggctcaggt tgatgacgat acgctcgaca cttattccgg
     1321 aaccggaatg atcttccttg cggggcggat gttcgacagc acgcggccgg tgccagaaga
     1381 gaaactgcaa agagaagttg cttatcagtg gtgggggcag acagtcggat tgaagtcctt
     1441 cgacgatgcg tggatctcgc agggcctggc ggaatggagc gcctttgcat ttcgtgaatc
     1501 tactttgaca ggcggcgcgc tcgacgctgc acagcgcgag cagcaagagc gggcgctcac
     1561 ttttgaacaa actgcttcaa tcgcgcgggc ccccggagcc ctggacgatc aatcggctgc
     1621 gtaccagtcg atcgtatttc acaaaggttc catggtgttt cgcatgttgc gcgagacgat
     1681 agggacagaa aaattcgatc aactgttgcg taacttcctg gagacttatc ggggcaagag
     1741 cgcgtcgatt gacgactttg aaaaactgac cacgcaggtt aacggcagca acatgcgtta
     1801 cttttttgcg caatgggtag aaggcaccgg agtgccggag tttaccgtcg actatcaaat
     1861 tatccgcaca agggccggca agttccgtac gcggggcacc gtcaagcata cgctgcagac
     1921 cctacgcatg ccggtgcaac tgatgctgcg ttccgaagga gatagtcaga caacaacgac
     1981 gacaattgag gaacgcagcg aggacttcga cttcgagtcg aacggccagc cgctggaagt
     2041 tgtagttgac cccaattacc gcattctgcg gatgtctgac gagttgcgag tctcgattat
     2101 cgcccggcgt ggaatcgagc agatgaagga aggactttac gcggaggcgc aacaacagtt
     2161 tgaagccgcg ctcaaattag accgctcgaa ttcgtgggtg tactacaacc ttgggttgct
     2221 ctttcttgag cagcgcaatt ggggccaggc gctcgacaac ttcgacgcgg cgctgaacgg
     2281 caccttgaaa ccatcctgga ttgaagtctg ggcgcgcatc aagcgtggca acgcttacga
     2341 tgcacagggc gagcgcagtc gcgcggtgag cgaatacaac aaggcagtgc agtcggg
//
LOCUS       DASUEX010000079         4051 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1049185, whole genome shotgun sequence.
ACCESSION   DASUEX010000079 DASUEX010000000
VERSION     DASUEX010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4051
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1049185"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(127..948)
                     /locus_tag="VFV61_01360"
     CDS             complement(127..948)
                     /locus_tag="VFV61_01360"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEX4899146.1"
                     /translation="MSTAIATEDVLIIETPERVPLHFALASIGNRFLACAIDHAIQVF
                     TIVLMIIAFATLANVSSVGDRLKAAPNWVYAVLIVILFLIVSGYFAFFEWLWSGQTPG
                     KRWLKLRVIREDGRPVTFWEATVRNLLRSFDMMPAPFYSIGLISVFVSSIDQRVGDLI
                     AGTVVVREREAEAPAFAQVFAAPISDPALRRAFPAVDFTASVNNLSEAQIEVVETFLR
                     RRWELGDAARQWMAWRVSIPLLFQMRPGYELSTFTYEGFLEELLHRYRAVHRFKE"
     gene            complement(980..1399)
                     /locus_tag="VFV61_01365"
     CDS             complement(980..1399)
                     /locus_tag="VFV61_01365"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEX4899147.1"
                     /translation="MSDENKALIHRWFEEVWNKGREEAIDEMFAADGVAHGLTEEGDR
                     ALRGGGEGFKPFFRTFRSAFPNIEVVVEDTVAEGDKVAARCSVRGKHQSDSLGFAATG
                     AATEFTGITIVRIADGKIVEAWNNFDFMTMYKQLGAL"
     gene            complement(1643..2869)
                     /gene="tyrS"
                     /locus_tag="VFV61_01370"
     CDS             complement(1643..2869)
                     /gene="tyrS"
                     /locus_tag="VFV61_01370"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEX4899148.1"
                     /translation="MTAMTIDEQLEFLRKGTVEIIRENELRAKLEKAAKSGKPLRVKL
                     GVDPTAPDIHLGHTVVIRKLRAFQELGHTVIFLIGDFTGLIGDPSGNSATRPQLTREE
                     INANAETYKAQIFKLLDPAKTEMRFNSEWMDKLGSDGFIRLAAHVTVKQILERDDFAK
                     RLNEEKPIALHELLYPLTQAYDSVALQADVELGGTDQKFNLLMGRNLQREYQQEAQVC
                     VVMPLLEGTDGVQKMSKSLGNYIGISEPPAEIFGKVMSISDDLMWRYSELLTDLTMPE
                     ISSWREATASGEINPRDVKVALAKRIVADFHSGAAADAAEEDFVRRFRQKETPDDVEE
                     RSVPSNSGNWDLSQLLVTVGMAESKAAARRLIDQGGVSIDGEKQTLANSATPSQTGRS
                     ILIKVGKRRFVRVNFS"
     gene            3069..>4051
                     /locus_tag="VFV61_01375"
     CDS             3069..>4051
                     /locus_tag="VFV61_01375"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX4899149.1"
                     /translation="MRILSFAAATVFCGVACTFYGVESLDTTSIVQQKSAIEPSSDIK
                     ANIQKLSSANARERAEAACKLGKARAEAAIPGLIQLLSDDTPVEQPICEEKGNWRGKG
                     NNTTPGEMAAVALSQIGTEAVEPLIAALRSNAWQARANAAFALGLIHDDRVVEPLISA
                     TRDPEARVREKAAWSLGLVGDDRAVEPLSAALKDGDARVRSQAAWALGLKGDERSVEP
                     LILALRDQNEEVQSQAAWALGLKGDDRAVEPLTIALTANNEEVRSQAAWALGLKGNSN
                     AVEALIQALRDPNAEVRSQAAWALGLKGDARAVEPLRAALNDPDSDVRKQAR"
BASE COUNT          936 a         1127 c         1085 g          903 t
ORIGIN      
        1 cgattgccga ttggcgattg ggtgtggaat ccagtcaaat cgaattccct ccggaagatt
       61 aggttgggtg gcttggttct aaatgacgat tgacattggc gattggcaat cgccaatctt
      121 ctcagctcac tccttgaagc gatgcacggc acggtaacga tgtagcagtt cctctaaaaa
      181 gccttcatag gtgaacgtag acaattcgta gccgggccgc atctgaaata gcagcggaat
      241 cgatactcgc cacgccatcc actgccgcgc tgcgtcgccg agttcccaac gccgtcgcag
      301 gaaggtttct actacttcaa tctgtgcctc actcagatta ttaacactgg cggtaaagtc
      361 gacagcagga aacgcccggc gcagagccgg atcgctaatg ggggcagcga atacctgagc
      421 aaaggccgga gcttcggctt cgcgttcacg caccacaacg gtacctgcaa tcaaatctcc
      481 aaccctttga tcaatgctgc tgacaaagac ggagatcagg ccgatcgaat aaaacggcgc
      541 gggcatcatg tcgaaacttc gcagcaagtt tctgactgtt gcctcccaga atgtcacggg
      601 cctgccgtcc tcgcgaatta cgcgcagctt cagccaacgc tttcctggcg tctggccact
      661 ccaaagccat tcaaagaaag caaaatagcc tgacacgatc agaaacagga ttacgatcag
      721 aactgcataa acccagttcg gcgccgcttt caacctatct ccaacactgg aaacgttcgc
      781 gagcgtggcg aaggcgatga tcattaggac gatggtaaag acttgaatag cgtggtcaat
      841 cgcacaagcg agaaaacgat tgccgatcga cgcgagcgcg aaatgaagcg gtacgcgctc
      901 aggcgtttcg ataatcagga cgtcttctgt ggcgattgcc gtagacattg cttgtcgatc
      961 gctgtttatc tgtggcggac tagagcgcgc cgagctgctt gtacatagtc atgaagtcga
     1021 agttgttcca ggcctcgacg attttgccgt cggcaatgcg gacaatggtt atgccggtaa
     1081 actcagtggc tgctccggtc gcagcaaagc ctaaactatc ggattgatgc ttcccgcgaa
     1141 cggaacaacg cgccgcgacc ttgtcgccct ccgcaacagt atcttcaacg acgacttcaa
     1201 tgttgggaaa cgccgatcgg aaggttcgga aaaaaggctt gaatccctcg ccgcctccgc
     1261 gcagggctcg gtcaccttct tcagtcaggc catgcgccac cccatccgcg gcaaacatct
     1321 cgtcaatggc ttcttcgcga cctttgttcc acacctcctc aaaccaacga tggatgaggg
     1381 ctttgttttc gtcagacatg ttcggtcacc tctcattctt tcacattaaa agggtcacgc
     1441 agttgatcgt gcgagtcgtc ttcaatctca ttctcaccgc tcttcagcgc ggtgcgtgtt
     1501 tgccaatcga gaatcgaaac cgtttaaacg atttccctac atcaacgtca actttgccat
     1561 cgggctcaag cccggtgaga atgagagaag aaaatcatta ccagtgttac gtgttccagg
     1621 aatcctgcaa ggctaagact ccttaggaga agttgacgcg cacgaagcga cgcttgccga
     1681 ctttgatcaa gatcgacctg cctgtttgcg atggcgtcgc agagttagcc agggtttgct
     1741 tttcaccatc aattgagacc cccccctggt cgatcagtct tcgtgccgcg gctttcgatt
     1801 ccgccattcc cacggtcacc aatagttgtg agagatccca attcccggag ttggagggca
     1861 ctgatcgttc ttccacatca tcgggcgttt ctttctggcg aaagcggcgc acaaagtctt
     1921 cctcagcagc atcggctgcg gccccggaat gaaagtccgc aacgattctc ttcgccaggg
     1981 ctaccttaac atcacgagga ttgatctccc cgctagcagt agcctcgcgc caggatgaaa
     2041 tctcaggcat agttagatca gtcaggagtt cggaatagcg ccacatgaga tcatctgaga
     2101 tggacatcac ttttccgaag atctccgctg gaggctcact gataccgatg tagttgccga
     2161 gactcttcga catcttctgg acgccatctg ttccttccaa caaaggcatc acgacgcaaa
     2221 cttgcgcttc ttgttgatat tcacgttgca gatttcttcc catcaacaag ttgaactttt
     2281 gatctgtgcc accgagctcg acgtccgctt gcaaagcgac cgagtcgtag gcctgcgtaa
     2341 gtgggtagag caactcgtgc agcgcaatcg gcttctcttc gtttaagcgc ttggcaaagt
     2401 cgtcgcgctc gaggatctgt ttgacggtga cgtgcgctgc cagccggatg aaaccatccg
     2461 aaccgagttt atccatccat tccgagttga agcgcatttc agtcttcgcg ggatcgagga
     2521 gtttgaagat ctgcgctttg taagtctcag cgttcgcgtt gatctcttcc cgagtcaatt
     2581 gcgggcgggt ggccgaattt cctgagggat caccaatcag tcccgtgaaa tcgccaatca
     2641 gaaaaatcac cgtatggccc agctcctgaa acgctcgcaa cttgcgaatg actacagtgt
     2701 gccccagatg gatatcgggc gcggtgggat ccacgccgag tttgacgcgc agaggttttc
     2761 cactctttgc cgctttctcg agtttggcgc gcaactcatt ctcgcgaatg atctcaacgg
     2821 tccccttgcg caggaattcg agttgttcgt cgattgtcat tgctgtcatc gtaaatcgta
     2881 aatcgtgaat cgtaaatcgt gaatcgggtc gcgctaaatc aaaaacgcct tcgctcccga
     2941 cacgtcagga ctctgcccgg tgcactgcaa agttgttggc acaaaccccg gggcggtggt
     3001 gtttgcttac cagaacacct ctgtgcgtcc ctttttgaaa gcagatctac gaatcgaagg
     3061 agcttgcaat gagaatacta tcattcgccg ccgcgactgt tttctgtgga gtggcttgta
     3121 ccttctacgg tgtcgagtct ttggacacca ccagcattgt ccaacaaaag agcgcgattg
     3181 agccctccag cgacatcaaa gccaacattc aaaaactcag ctccgcaaat gccagggaac
     3241 gcgccgaggc cgcctgtaag ctcggtaagg cccgggcaga ggcggcgata cccggcttga
     3301 tccagttact cagcgacgat acgccagtcg agcaaccgat ctgcgaagaa aaaggaaatt
     3361 ggcgcggcaa gggcaataac acaaccccag gcgagatggc ggcggttgcg ttgtcccaaa
     3421 tcggcactga agcggttgag ccgctcatcg ccgcgttgcg aagtaatgcc tggcaagcac
     3481 gagcaaatgc ggcatttgct ctcgggttga tccacgacga ccgggtggtt gaacctttga
     3541 tttcggccac tcgcgatccg gaagcgcgtg tgcgcgaaaa ggccgcgtgg tcgctgggac
     3601 tggtcggcga cgatcgggct gtcgaaccgc ttagcgccgc cctcaaagac ggcgacgccc
     3661 gcgtgcgttc gcaagcagcc tgggccctgg gtttgaaagg tgacgagcgt tcggtcgaac
     3721 cattgattct cgcgctgcgt gatcaaaatg aagaggtgca gtcacaagcc gcctgggccc
     3781 ttgggctcaa gggtgacgat cgcgcggtgg aacctctgac gattgcgctg acagcgaaca
     3841 acgaagaagt tcgctcccaa gcggcctggg ccttgggcct taagggcaac tccaacgccg
     3901 ttgaagcatt aattcaagcg ctaagagatc caaacgcgga agtgcgttcg caagcggcct
     3961 gggctttggg actgaagggc gatgcacgcg cagtggaacc gttgcgcgct gctttgaacg
     4021 atccggattc ggacgtaagg aaacaagcac g
//
LOCUS       DASUEX010000080         7307 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_706046, whole genome shotgun sequence.
ACCESSION   DASUEX010000080 DASUEX010000000
VERSION     DASUEX010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7307
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_706046"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="VFV61_01380"
     CDS             complement(<1..546)
                     /locus_tag="VFV61_01380"
                     /inference="COORDINATES: protein motif:HMM:NF026257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX4899150.1"
                     /translation="MSATLEASEVLTSSFVAARPEYALLLTCARASLEAAQKKQVQDL
                     SAQLLDWDLVLQLADRHGLQPLLHWHLNEICPDAVPAAPRQHLRVAFQRVSALNILLT
                     HELQKLLGLFADHNVQAVPYKGPALAMQLYGNVALRQFSDLDILVHPRDVLRARDVLV
                     AEGYAQLPPLTDGQQAVLLRTQ"
     gene            complement(608..2407)
                     /gene="lepA"
                     /locus_tag="VFV61_01385"
     CDS             complement(608..2407)
                     /gene="lepA"
                     /locus_tag="VFV61_01385"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HEX4899151.1"
                     /translation="MNVEQIRNFSIIAHIDHGKSTLADRLLELTGAVTGRDMSEQVLD
                     NMDLERERGITIKSHAVRLDYKAEDGKDYVLNLIDTPGHVDFSYEVSRSLSACEGALL
                     IVDASQGVEAQTLANTYLALENNLELIPVINKIDLPGAEPDRAVEQIEHLIGLETHNA
                     VFTSAKTGAGVHEVLEAIVRDVPSPKGDPEAPLKALIFDSWYDSYRGVIVLFRVIDGM
                     IRPGMKIRFFNTGRDYQVETLGVNRPRPTPIDKLGVGEVGFLTASIKEVADVQIGDTI
                     TESARPTSEPFPGFEEIKPMVFAGLYPVDTNQYEELRDALDKLRLNDASFFYEPESST
                     ALGFGFRCGFLGLLHMEVVQERLEREFNLDLITTAPGVRYVVHTTDGKVVEIDSPAKM
                     PDPGRIVKIEEPIIRATILTSDEYLGGILPLLEEKRGVQKGFEYIAANRVMLTYELPL
                     NEIVLDFYDRLKSVSRGYASLDYNLAGYWESDLVKLDILVADEPVDALSLVLHRSTSQ
                     AKGRALAEKMKQLIPRQLFEVPIQAAIGNKVIARETVKSLGKNVIAKCYGGDITRKRK
                     LLEKQKEGKKRMKKVGRVEIPQEAFLAVLKVNE"
     gene            complement(2588..3085)
                     /locus_tag="VFV61_01390"
     CDS             complement(2588..3085)
                     /locus_tag="VFV61_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899152.1"
                     /translation="MNRNQKIAIGCGGAGCLGLIVLAVAGVVGYFYMNSSSLANRNSD
                     SNFNFNTNLNTNSSDNSSDNSSDNSSTNSSDNSNTTADDATSSSSMSDDDKHKLFQAA
                     GITHDSQLILKVLTKIGFPSGTGEDYEKFSKEHSSWAMKNLEFMQSVNTPEKGRAYVE
                     AHLDD"
     gene            complement(3279..4661)
                     /locus_tag="VFV61_01395"
     CDS             complement(3279..4661)
                     /locus_tag="VFV61_01395"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX4899153.1"
                     /translation="MPGSQSHLKGTVLLVDDDEAVRRGLYWALNSDYRVIEAGSRGEA
                     VEMLQHEKVDVVISDLHLPPHLDDISEGLTVVQAARSMRPAVQAVVITGSDSKHAALE
                     AVKHGAYGFFEKPLDADEVLHIVNQAARMRRLELENVRLREELLGSRGFSHLIGGSQS
                     LERIVKHARTVAATSATVLIIGENGTGKELLARAIHEESPRAAGPFIGVSCAALPETL
                     IESELFGHEKGSFTTATQTRKGRFELADGGTLFLDEIADLSPTVQVKLLRVLQERSFE
                     RVGGTKTLHVDIRLVAASNCDLEQEVAAGKFRQDLFYRLNVVPLVLPTLRARKEDVPI
                     LATHFAEKAAGKYLRPAPVLDPLLLEALQEYEWPGNVRELENVMERLVVLTNGPTIDL
                     EFLPEKMLRVLPGTNLADEGTLEGGVAALKKRMILKALEASAGNKVAAAKLLGISRSY
                     LHRLISEFEL"
     gene            complement(4744..6330)
                     /locus_tag="VFV61_01400"
     CDS             complement(4744..6330)
                     /locus_tag="VFV61_01400"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX4899154.1"
                     /translation="MTGLPDQQEEELLRVARSQALIQFLTSREQRTNANAKTLESNLR
                     GTLASLLNRQSHTVAIAGYDQGKRKMFFAERSADELIIREKDFLPGQLQPDERIWTAS
                     GPFRSAVSSNPLRASVQLTASVLPTAAIPFGAVVSEVELDSLLREAAGADWSDTSREI
                     VVTDESGRVLYYSNPAMLRRQVSIFSSQGRLTGPTKISGLNLLVHTLDRSPASLSAQQ
                     IGIGALLLSCFFATLAAFLLARFWEKRTRGIERVSAGVAAIAHGDLNHRIEALSSDAL
                     RPISDNLNLMTAQLREQIAREAEARQFQSFVRLSAMLTHDLKNAIEALSLIVSNMERH
                     FDNEAFRADAMKSLNLATQNLRSLVDRLTNPVTTLSGEYKRPQPVDLIPLLARVIKLT
                     AAPALSAQQIETKLPDSLPALVDAERIVKVIENLILNAVEAMAGTEGKLLSVAAGTAE
                     DGKVFFSISDTGAGMSPSFIERQLFRPFATTKKRGVGLGLYTCREVVRANGGAIEVDS
                     REGAGTTFRVVLPSAAIEKV"
     gene            complement(6574..>7307)
                     /locus_tag="VFV61_01405"
     CDS             complement(6574..>7307)
                     /locus_tag="VFV61_01405"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HEX4899155.1"
                     /translation="NDREPVTEADRVANDLIVSSLQREFPDDGILAEESVDTSHRLDK
                     ERVWMIDPLDGTNGFIDGNGDFAVQIGLVENGEAILGVVYQPLSGVMYRAVRGGGCWI
                     ERPNVTPERATVSQQNELHRMRLAASRSHRSPRMDRVVNAFAVKEEVRRGSVGIKVGL
                     ICERQCDLYVHLSPRTKQWDTCAPEIILHEAGGRITDLFGQPLRYNVAEVQNRNGLVA
                     SNGVVHEEIISALVPLLDEFGRVAV"
BASE COUNT         1666 a         2023 c         1939 g         1679 t
ORIGIN      
        1 ctgagttcgc aacaatactg cctgttgacc gtcagtaagt ggcggcaatt gcgcatagcc
       61 ctcggcaaca agtacgtcgc gagctcgtaa cacgtcgcgc gggtgcacaa gaatatcgag
      121 gtcgctgaac tgacggagcg cgacattccc gtaaagctgc attgccaggg ccggaccttt
      181 ataaggcaca gcttgcacat tgtggtcggc aaataacccg agcagttttt gtagttcgtg
      241 agtgaggaga atgttcagcg cggaaacgcg ttgaaacgcg actcgcagat gctggcgcgg
      301 cgcagcaggc acagcgtccg gacaaatctc gttcagatgc cagtgcagca agggctgcag
      361 gccgtggcga tctgccaact gcaaaacgag atcccagtcg agcagttgcg ctgagagatc
      421 ctgaacttgc tttttttgtg cggcctcgag agacgcgcgc gcgcaggtca gtagtaaggc
      481 gtactcagga cgcgccgcaa caaaactgct tgtcagaacc tctgatgctt caagagtcgc
      541 agacatgaaa ttacaggtag gaaattgagc agggctcaaa agaggcggat gacaactcgc
      601 ttaagactta ctcgttcact ttcagaactg cgagaaaagc ttcctgcggg atctctacac
      661 gaccgacttt cttcatccgc ttcttgcctt ctttttgctt ctctaacagt ttgcgcttgc
      721 gcgtgatgtc gccgccgtag cactttgcaa tcacgttctt gccgagtgac tttactgtct
      781 cacgcgcgat gaccttatta ccaatcgccg cctgaatcgg cacctcaaaa agctggcgcg
      841 gaatcaattg tttcatcttt tccgcgagcg cacggccttt cgcttgcgac gtgctgcgat
      901 gaagcacgag cgagagggcg tcgactggct catcggcaac caggatatca agcttcacca
      961 aatcgctttc ccagtaaccg gccagattgt aatcaagcga agcatagccg cgcgagactg
     1021 atttcaaacg atcgtagaag tccaggacaa tttcgtttaa gggcaattca taggtcaaca
     1081 tcacccggtt cgccgcaatg tattcgaaac ccttttgtac tccacgcttt tcttcgagca
     1141 gcggcagaat gccgcccagg tattcatctg atgtaagaat cgtggcgcga attattggct
     1201 cttcgatctt cacgatccgg cccggatcgg gcattttagc cggtgaatcg atttcaacga
     1261 ctttgccgtc agtggtgtgc acgacgtaac gaacgccggg ggcggtggta attaggtcga
     1321 gattaaactc gcgctccaaa cgctcctgca ccacttccat gtgcagcaag ccaagaaagc
     1381 cgcagcgaaa tccaaagccg agcgccgtcg atgattccgg ctcataaaag aacgacgcat
     1441 cgttgagcct taacttgtcc aacgcatcgc gaagttcttc gtattgattt gtgtccaccg
     1501 gataaagacc ggcgaagacc atcggcttta tctcttcaaa gccggggaac ggttccgacg
     1561 tgggacgcgc actctcggta atggtgtcgc cgatttgcac atctgcaact tctttaatcg
     1621 aggccgttag gaatccgact tcgcccactc ccagcttgtc gatcggcgtg ggccggggac
     1681 gattaacccc cagggtctca acctggtaat cgcgtccggt attgaagaac cgaatcttca
     1741 ttcccggtcg aatcatgccg tcgatcacgc gaaacaaaac aatcacgccg cgatacgagt
     1801 cgtaccacga atcgaagatc agagctttga gcggagcttc cggatcacct ttcggcgaag
     1861 gcacgtcgcg cacgatagct tccagcactt catgcacgcc cgcgccggtc ttggcactgg
     1921 tgaagaccgc attgtgggtt tccagaccaa tcaaatgttc gatctgttcg actgcccgat
     1981 caggttcggc gcctggcaag tcgatcttat taatgacggg gatcagttcc aggttgtttt
     2041 caagagccag gtaagtgttg gcgagagtct gcgcttcgac accctgcgaa gcgtccacaa
     2101 tcaacaacgc accttcgcat gctgaaagcg agcgtgagac ttcataagag aaatccacgt
     2161 ggcccggcgt gtcaatcaga ttcagaacat aatccttccc atcctccgct ttgtaatcga
     2221 ggcgaacggc gtgcgactta atcgtgattc cgcgctctct ttcgagatcc atgttgtcga
     2281 gcacctgctc cgacatatca cgtccggtca ccgcacccgt caactcaagc agccgatccg
     2341 caagagtgga tttcccgtga tcgatgtgcg cgatgatgga aaaattacga atctgttcga
     2401 cgttcatcgt taattcttac ttagcgcttt gtacttggta ctttgttcgc gtgcccagct
     2461 tgatcggttg attgataaga caagcttagc gatcaaacaa agtacaaagg acaaagtaca
     2521 aagaacgttt tacgaaaacg ggcgcgaaac cgcacgcgcc cggatcgcct gcaattgaaa
     2581 agtgtaacta atcgtccagg tgggcctcga cgtacgctcg tcccttctct ggcgtgttta
     2641 ccgattgcat gaactccaga ttcttcatgg cccaggagga atgctctttc gaaaacttct
     2701 catagtcttc cccggtaccg gaagggaagc caatcttcgt gagcactttc aaaatcaact
     2761 gggagtcgtg tgtgatgccg gctgcttgaa acagtttgtg cttgtcgtcg tctgacattg
     2821 aagacgacga agtggcgtcg tcagcagtcg tattgctgtt gtcgctgctg ttagtgctgc
     2881 tgttgtcgct gctgttgtcg ctgctgttgt cgctgctgtt ggtgtttaga tttgtattga
     2941 agttgaaatt gctatcgcta ttccggttgg caagcgatga cgagttcatg tagaagtaac
     3001 cgacaacgcc ggcgaccgcc aaaacgatca agccgaggca accagcacct ccgcagccaa
     3061 tggcaatttt ttgatttctg ttcatctttt cctcgttggg ttggggtgac tagctggaga
     3121 aatagagtag cacgcgccgt ccattgtagg ggtgtcccgc cgcgggcgcc caataatatg
     3181 ccggaaagcc ttcttccgtt gagctcggaa ttggttcgca tgtgcgtaca cagagggaag
     3241 ctactacaga attaggagct ctcaactaag cgtcctgttt acaattcaaa ttcactgatc
     3301 aagcgatgca agtatgaacg gctgataccg agcagtttgg ctgccgccac tttgttccct
     3361 gcagacgcct ctaacgcttt caggatcatg cgctttttga gtgcagccac tcccccttcc
     3421 agcgtgcctt catcggcaag atttgtcccc ggaagaactc gcaacatttt ttccggcagg
     3481 aactccaaat caatcgtggg tccattagtc aggaccacca gtcgttccat gacgttttcc
     3541 agctcgcgca catttcctgg ccactcatat tcctgtaacg cctccaggag caatgggtcg
     3601 agaacgggcg cgggtcgcaa atactttccg gccgccttct cggcgaaatg agttgccagg
     3661 atcggtacgt cttctttgcg cgcacgcaac gtaggcaaaa ccaaaggcac tacattcaga
     3721 cggtagaata aatcctggcg aaacttgcct gccgcaactt cctgttccaa atcgcaattg
     3781 ctcgcagcca ccagtctgat atcgacgtgc aaggtcttgg tgccgccgac ccgttcaaaa
     3841 gatcgctctt gcaagactcg caacagcttc acctgaaccg tgggcgagag atcggctatc
     3901 tcatcgagaa agagtgtgcc gccgtcggcc agttcaaaac gacccttgcg tgtttgcgtt
     3961 gcagtagtga aggagccttt ctcatggcca aacaattccg actcgatcag tgtctcgggt
     4021 agcgcggcgc aactcactcc tatgaacgga ccggcagctc taggactctc ctcatgaata
     4081 gcgcgtgcca gcaattcttt gccggttccg ttctcaccga tgatcagcac tgtggcactg
     4141 gtcgcggcca ccgtgcgggc gtgtttgacg attcgttcga gagactggct gccgccgatc
     4201 aggtggctaa acccgcgtga gcccagcaac tcctcgcgca atctcacgtt ctcaagttcc
     4261 aagcgtcgca ttcgtgccgc ctgattcacg atgtgcaaca cttcgtcggc gtcgagcggc
     4321 ttttcaaaaa agccataggc gccatgcttc actgcctcga gcgcagcgtg ctttgagtcg
     4381 cttcccgtga tgacgacggc ctgaacggcg gggcgcatgc tacgtgctgc ttgcactacg
     4441 gtaagccctt cggagatgtc atcaagatgc ggcgggagat gaaggtcgct gataactacg
     4501 tccaccttct cgtgttgcaa catttccacg gcttcgccac gcgagccggc ttcgatgacg
     4561 cgatagtccg agttcagggc ccagtaaagt ccccgacgga ccgcctcgtc gtcatcaacc
     4621 agcagcactg ttccctttaa atgtgactgt gaaccgggca tcacaagtac gagccagcgt
     4681 cccgcctgac tgaactaaga ctgtggattt gacgcgagta aacgggaagc gaacctgcca
     4741 gcgctagact ttttcgatgg cggccgatgg taacaccacg cgaaaggttg tgccagcacc
     4801 ttcacgcgaa tcgacttcaa tcgcgccccc attcgcgcgc acgacttcgc ggcaggtgta
     4861 gagtccgaga ccgactcccc ttttcttggt ggtggcaaag ggacgaaaga gctgtcgctc
     4921 gatgaagctg ggggacatac cagcgcccgt gtcgctgata ctaaaaaaga cttttccatc
     4981 ctcggcagtt ccggcggcaa cagaaagcag cttcccttca gttccggcca tggcttcaac
     5041 ggcgttgagg atcaaattct caatcacctt tacaatgcgc tcggcatcaa ccagcgccgg
     5101 tagcgagtca ggcaacttcg tttcgatctg ctgggcactc agtgcgggtg cggcggtgag
     5161 cttaataacc cgggctaata atggaatgag gtcgacaggc tgcgggcgtt tgtattcacc
     5221 gcttagggtg gtgactggat tcgtcaggcg atcaaccaga gaacgtaggt tttgggtagc
     5281 gaggttcagc gacttcatcg cgtctgcgcg aaaggcttcg ttatcgaagt ggcgctccat
     5341 attactgacg atcagggaca acgcctcgat ggcgtttttc agatcgtggg tcagcatcgc
     5401 cgagagtctg acgaaggatt gaaactggcg ggcttccgct tcccgcgcga tctgttcgcg
     5461 caattgcgcg gtcatcagat tgagattgtc ggagatggga cgaagagcat cactcgagag
     5521 tgcttcgatg cgatgattga ggtcgccatg cgcaattgcg gcgacgcccg cactaacgcg
     5581 ctcaattccc cgagttcgtt tttcccaaaa ccgtgccagc aggaatgcgg cgagagtcgc
     5641 aaaaaagcaa gagagaagca gcgctccgat tcctatttgc tgcgctgaaa gactcgctgg
     5701 agatcgatcg agtgtgtgca ctagaagatt gagtccggag attttcgttg gacctgtcaa
     5761 acgcccctgg ctgctgaaaa ttgacacttg tcgtcgcagc atcgctggat tcgagtaata
     5821 gagaactctt ccgctctcat ctgtaacaac aatttcacgc gacgtatcgc tccaatcagc
     5881 acctgccgcc tcgcgcaaga gtgaatcaag ttctacttca ctaactaccg ctccgaacgg
     5941 tattgccgca gtgggcagta ccgaagcggt caactgcaca cttgctcgca agggattact
     6001 ggataccgcg gagcgaaagg ggccgcttgc tgtccagatc cgttcatccg gttgcaattg
     6061 accagggagg aaatcttttt cccggattat caattcatcg gcacttcgct cagcgaagaa
     6121 cattttgcgt ttgccttgat catagccggc gatcgctacg gtatgacttt ggcggttcag
     6181 cagcgaagcc agcgtgccgc gcaggttcga ctccagggtt ttggcatttg cattggtccg
     6241 ttgctcacga gacgtaagga actggatcag cgcctgcgaa cgggccacgc gaagcaactc
     6301 ctcttcctgc tgatcaggta gtccagtcac cctttcgcta aacttagtga tcgcgacatc
     6361 tccatcaacc ctgcgcgtcc ccaatcgtcg cgcggcccaa tagctaaagc cggaaactag
     6421 aagaagtggt aacaggaata gaatgaggaa agtgagaaaa ttttttggtc gcagtcgcaa
     6481 gctgaggagc tccgtgagtg attcagatgt cagatgtaag tagtaagtag taagtagtca
     6541 gaaagagcca gtgcaatcgg caatgggcaa tccctagacc gccacgcggc caaactcatc
     6601 cagcaacggc accagggcac tgataatctc ttcatgcacg acgccgttgc tggcgacgag
     6661 tccattgcgg ttctgaactt ccgccacgtt atagcgcaaa ggctgaccaa acaaatccgt
     6721 gatgcgcccg ccagcctcgt gcaggatgat ttcgggggcg caggtgtccc actgctttgt
     6781 gcgcggcgag agatgtacat agaggtcgca ctgccgttcg cagattagcc cgacttttat
     6841 tcccaccgaa ccgcgccgca cctcttcctt aaccgcgaac gcgttgacga cgcgatccat
     6901 acgcggactg cgatgcgacc ggctggccgc caatctcatg cggtgtaact cgttctgttg
     6961 ggaaaccgta gcgcgttcag gcgtgacatt cgggcgctca atccaacagc ccccgccgcg
     7021 aactgcccga tacatgacac cgctcaacgg ttgatagaca actcccagga tagcttcgcc
     7081 attttccaca agacctattt gtacggcgaa atcgccgttc ccatcgatga agccgttggt
     7141 gccgtcaagt ggatcgatca tccagacgcg ttccttatca agccggtgcg aggtgtcgac
     7201 tgattcttcc gcaaggatac cgtcatcggg aaactcacgt tgcaaggaac taacgattag
     7261 atcattggcc acacgatcgg cttccgttac tggttcacgg tcattga
//
LOCUS       DASUEX010000081         2473 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_935336, whole genome shotgun sequence.
ACCESSION   DASUEX010000081 DASUEX010000000
VERSION     DASUEX010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_935336"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..740
                     /gene="priA"
                     /locus_tag="VFV61_01410"
     CDS             <1..740
                     /gene="priA"
                     /locus_tag="VFV61_01410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEX4899156.1"
                     /translation="VGEGTEQIEALLTKLFPTLRIARIDRDTATRRGAFEKSLFDFSA
                     GKIDMLVGTQMLAKGHDFPNVTLVGVVSVDAGLALPDFRAAERTFQLITQVAGRAGRG
                     ERPGRVLIQTYHPNHYALRHACAQDYSGFYQEEIRHRQNHGYPPFVSLASLLLHGPAL
                     GKTRADALELRKELDLANQERSCRILGPAPAPLARLKGEHRIQLLVKSRSRRQLRKVV
                     DTAMAAVAGRGQNLRSINLEIDPVSIM"
     gene            782..1822
                     /gene="lpxD"
                     /locus_tag="VFV61_01415"
     CDS             782..1822
                     /gene="lpxD"
                     /locus_tag="VFV61_01415"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HEX4899157.1"
                     /translation="MKLSELAALTSARLEGDANTEIIGAAGLDEARSGYVSFLANPRY
                     TPRVNETAASAIYVAEDAEIERKISLLRAKDPYLAYTRALRLFHPEPSLEPKIHETAV
                     IDETATVARDVCIGACTVIGPRAKIERGVRLYPNVTIYEDVTIGEDSVIHSGTVVREY
                     SVIGKRVIIHNNVVVGCDGFGYAKDEERRWLKIPQTGRVLIGDDVEIGAGTTIDRASV
                     GESRIGRGTKIDNLVQIGHSCTVAEDSLLCAQVGLAGSSHIGSRVILAGQAGVAGHLK
                     IGDDVVMTAKSATSHDIPAGKVISGIPAFDNKDWLRSTAAFRRLGEMQRTLRDLARRL
                     AAIEDSESGPES"
     gene            complement(1977..>2473)
                     /locus_tag="VFV61_01420"
     CDS             complement(1977..>2473)
                     /locus_tag="VFV61_01420"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX4899158.1"
                     /translation="IDILVNNAAINRGDNFATTTDRAWDEVINTNVGSLFAVTKPIYK
                     QMIRQRKGSILNITSIGAIRALPTAVHYATSKAAMIGFTKCLSREAGTFGVKVNAIAA
                     GIFDTELAQALPERLLQMHDIWVSAGRLGKPEELAEYAAFMVSDRNSFMNGEVVIVDG
                     GTVT"
BASE COUNT          562 a          678 c          686 g          547 t
ORIGIN      
        1 acgtcggcga gggtaccgaa caaattgagg cgctgttgac gaaactgttc ccaactctgc
       61 gcatcgcccg gattgatcgc gatacagcaa cgcggcgagg tgcgtttgag aagtccttat
      121 tcgatttcag cgccggcaag atagacatgc tggttggtac gcaaatgctc gcgaagggtc
      181 acgacttccc taacgtcacg ctcgtcggag tggtctcggt tgatgccggt ttagcactac
      241 cagattttcg tgcggccgaa aggacattcc aactgattac tcaagttgcc ggtcgagccg
      301 gccgcgggga gcgtcctggt cgagttttga tccaaaccta tcatccaaac cactacgcgc
      361 tccggcatgc ctgcgcgcag gattattccg gcttctatca agaagagatt cgccatcgcc
      421 agaatcatgg ctatccaccc tttgtctccc tggcatcgct gctgctccac ggtcccgctt
      481 taggaaagac gcgagcagac gcacttgaac tacgaaaaga actcgatctc gccaaccagg
      541 aacgaagttg cagaattctg ggcccggcgc cggcgccact ggcgcgactc aagggcgaac
      601 atcgtattca gctgttagtg aagagccgca gccggcggca gttacgcaaa gtcgtcgaca
      661 cggccatggc cgcggtcgct ggtcgtgggc aaaacctccg ctcaatcaat ttggaaatcg
      721 acccggtttc aataatgtga agccgtgcgc ggcgccgtcc taatgagcta aactcacgcg
      781 catgaaactt tccgaacttg ccgcgctcac cagcgcgcgc ctcgagggtg atgcaaacac
      841 ggaaattatc ggtgctgcgg gcctcgacga ggcccgttcc ggctacgtca gcttcctggc
      901 gaatccccgt tacacaccgc gggtaaatga aacagcagct tcggcgatct atgttgcgga
      961 agacgcggaa atcgagagaa agatttccct cttacgcgcc aaagatccct atctcgccta
     1021 cacgcgcgcg cttcgcttgt tccaccctga accttccctc gaacccaaaa tacatgagac
     1081 ggctgtcatc gatgagactg ccaccgtcgc aagggacgtt tgcattggcg cgtgtacggt
     1141 gatcggcccg cgagccaaaa tcgagcgtgg tgtgcgcctc tatccaaatg tgactatcta
     1201 cgaggatgtc actataggag aagattcagt tattcactcc ggaacggtgg ttcgagagta
     1261 ttcagtaatc gggaagcgtg tgatcatcca taacaatgtg gttgtgggtt gcgacggttt
     1321 cggatacgca aaagacgaag agcggcgttg gctgaagatt ccgcaaaccg gacgagtgct
     1381 gatcggggac gatgtagaaa ttggcgccgg gactaccatc gaccgcgcct cggtcggcga
     1441 atcacgcatt ggacgcggca cgaagatcga taacctggtg cagattggtc actcatgcac
     1501 agtggccgaa gatagtttgt tgtgtgcgca ggtgggactg gcgggcagtt ctcatattgg
     1561 cagtcgcgtg atacttgccg ggcaggcagg cgttgccggc catctcaaga tcggcgacga
     1621 cgtggtgatg acagccaaga gcgcgaccag ccatgacatt cctgctggta aagtgatttc
     1681 cgggattccg gcttttgata ataaggattg gctgagatca acggctgcgt ttcgcaggct
     1741 tggtgagatg cagcggacgc tgcgagattt agcacgaagg ttggcagcga ttgaggattc
     1801 agaatccgga cccgaatcat agatcagatg gcagatttca gatggcagat gtcaggtttc
     1861 gggtttcagg tcttagatct caggtctcaa gtctcaagct cctgatctga catctgacat
     1921 ctaacatcta acatccaaca tctgaaacct gaaacctgat acccgccact ctcggtttaa
     1981 gtcaccgtac caccgtcaac gatcactact tcgccattca taaaactgtt ccggtcgctc
     2041 accataaagg cagcgtactc cgctaactcc tcaggcttcc ccagccgtcc ggcggatacc
     2101 caaatgtcgt gcatctgcaa gagacgttct ggcaacgctt gcgctaattc cgtatcgaag
     2161 attccggcgg cgatggcatt aactttgacc ccaaacgttc ccgcctcacg tgagaggcac
     2221 ttggtaaatc cgatcattgc cgccttggat gtcgcatagt ggaccgccgt tggcaaagcg
     2281 cgaatggcgc caatggaagt gatgttgagg atgcttccct tccgctgccg gatcatctgt
     2341 ttatagattg gttttgtgac cgcgaaaagg ctgcctacgt tcgtattgat gacctcgtcc
     2401 caggcccgat ccgtggtggt cgcgaagtta tcgccacgat tgatggccgc gttgttaacc
     2461 aggatgtcaa tcc
//
LOCUS       DASUEX010000082         3258 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_591850, whole genome shotgun sequence.
ACCESSION   DASUEX010000082 DASUEX010000000
VERSION     DASUEX010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3258
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_591850"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..991
                     /locus_tag="VFV61_01425"
     CDS             <1..991
                     /locus_tag="VFV61_01425"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX4899159.1"
                     /translation="VNRRNKMLYWEDYEWVRDHCNSCVEVGAQLNSGVDLKQDGIEFP
                     GARIIGVTSNMVDIEDKTMADGRFISGDEVERSALVCVLGGDIKDKYFPNSSAVGKTL
                     KIRGLPFRVVGVEEKRGSFFGDSFDRQVYIPVTTHQQIFGRNGLQIHGKGRDRATFQA
                     TIEEARVALRNKHRLKGNEEDDFGVVNVEDLNNQIDQFTGSIAAVVVPITLITLVVGG
                     IVVMNIMLVSVTERTFEVGLRKAVGATRKQILMQFLIESAILCALGGIIGLLLAWGVT
                     QLITALASITMTITIGYILLALIVSTAIGMLAGIYPAFKAARLDPILALTHAT"
     gene            995..2269
                     /locus_tag="VFV61_01430"
     CDS             995..2269
                     /locus_tag="VFV61_01430"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX4899160.1"
                     /translation="MKLRLPRGVLRETLMMALDSVRSHKFRSFLTVLGIVIGVMTAIT
                     IASLLTGLRQNIVAMIEEYGTNNIYAFHLSTGPRVSEDRSERLRKPLTIADAETIKVQ
                     ASAVEGVAVVAPNVGYSGGPFDDNVTYEGHNYRWGNTQGVTANYADLSNIALKEGRFI
                     SELDDQQRANVMVIGVNGADALFPGQQSNIVGTQVRMGGYNFEIIGVLEKRKAGFFGE
                     NEEDNAVFIPLRTAQKVAPARGYLLFVIRAHSGQVNEALTQAEEILRRRRNVKFDAPN
                     NFDIKTADKFIEQFDSITAMVGLIAIAISSLGLLVGGIGVMNIMLVSVTERTKEIGVR
                     KALGARRRDIVRQFLFEAMTLTFLGGILGVVLAVGISKLIMLLIPSLPASIPTWAVMS
                     GLIVSVGVGLIFGVWPARKASRLDPIECLRYE"
     gene            2512..>3258
                     /locus_tag="VFV61_01435"
     CDS             2512..>3258
                     /locus_tag="VFV61_01435"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX4899161.1"
                     /translation="MPKAQRDFIKEFLTYIQVEKGLARHTLESYGRDLARLQLWADKA
                     GKPLEQLTRADLRKWIANLSRAGLAPSSVARAVSAARGLFRFLMLDGHIKRHPTEDLD
                     TPQRFAYLPQFLTEDEINQLLAAPDIATAEGVRDRALIEVMYAAGLRVSELISLKQAD
                     VDMQSGLLICHGKGSKERRVPIGKSAIHWLQQYSTVRAGYGKPTSPQLFLNRGKPITR
                     QFAWALIKRYASKAGVKDISPHTLRHSFATH"
BASE COUNT          748 a          838 c          913 g          759 t
ORIGIN      
        1 cgtcaaccgt cgcaacaaga tgctctactg ggaagactat gagtgggttc gtgatcactg
       61 taattcctgt gtggaggttg gcgctcagct caacagcggt gtcgatctga aacaggacgg
      121 tatcgagttc cccggggctc ggatcatcgg tgtcacgtcg aacatggtcg acatcgaaga
      181 caagaccatg gcggacggcc ggtttatttc cggggacgag gtcgaacgct cagcgctggt
      241 gtgcgtttta ggtggcgaca tcaaggacaa gtacttccct aacagtagcg ccgttggcaa
      301 gaccttgaag atccgcggtt tgcctttccg cgtcgtcggt gtcgaggaaa agcgcggctc
      361 gttctttggc gattcattcg accgccaggt ctacattccg gtcacgaccc accagcagat
      421 cttcggacgc aatggtttgc aaattcatgg gaaggggcga gaccgcgcga ccttccaggc
      481 gacaattgaa gaggcccgcg ttgccttgcg aaacaaacac cggttgaagg gtaacgaaga
      541 agacgatttc ggtgtcgtga atgttgaaga cctaaacaat cagatcgatc agttcactgg
      601 tagcattgcc gcagtggtcg tgccgattac attgattaca ctcgtagtgg gcggcatcgt
      661 cgtcatgaac atcatgcttg tcagtgtaac ggaaagaacc tttgaagttg gtttgcgcaa
      721 agcagtggga gcaacgcgaa aacaaatcct gatgcagttc ttaatcgagt ccgcaattct
      781 ttgcgctctg ggcggcatta tcgggttgtt gcttgcctgg ggagtgacac aattgatcac
      841 tgctctcgcc tcgatcacga tgacgatcac gattggctac attctgctgg cactgatcgt
      901 ttccactgcc attggcatgc tggccggcat ctatccggca ttcaaggcag cgcgattaga
      961 tcctatcctg gcactgacgc acgcgacata agttatgaaa cttagattac ccagaggtgt
     1021 gctgcgcgaa acgctgatga tggcgctcga tagcgtgcgc tctcataagt ttcgcagttt
     1081 tctgaccgtg cttgggatcg tcatcggtgt tatgaccgcg atcacgatcg cgtcgctgct
     1141 gacaggttta agacaaaaca tcgtcgcgat gattgaagag tatggaacga acaacattta
     1201 cgcgttccat ctttccacgg gtccgcgcgt aagcgaggat cgttccgagc gcctccgtaa
     1261 accgttgacg atagcggatg ccgaaaccat caaagtgcaa gccagcgcgg tcgagggtgt
     1321 cgccgtcgtg gcgccgaatg tcggctacag tggcgggcct ttcgacgaca acgtcaccta
     1381 cgaaggacac aattaccgtt ggggtaacac ccagggagta actgcaaatt acgcagacct
     1441 ttcgaacatc gccttaaaag aagggcggtt catcagcgag ctggacgacc aacagcgagc
     1501 gaacgtaatg gtgattgggg tcaacggcgc ggatgcgctc ttcccgggac agcaaagtaa
     1561 catcgttggc actcaggtgc ggatgggtgg ctacaacttc gaaattattg gtgtcctgga
     1621 gaaacgcaag gcaggcttct ttggcgagaa cgaagaagat aacgccgtgt tcattcccct
     1681 tcgtacggcg cagaaggttg caccggcacg cggttacctg ctgttcgtca ttcgggccca
     1741 ttccgggcaa gtgaatgaag ctttaaccca ggcggaagaa atcctgcgac ggcggcgtaa
     1801 tgtgaagttt gacgccccta acaatttcga tatcaaaacc gcggacaagt ttatcgagca
     1861 gttcgacagc atcacggcaa tggtgggctt gatcgcgatc gcgatctcaa gtctgggcct
     1921 gctcgtgggc gggattggtg ttatgaacat catgctcgtg agcgtcaccg aacggacgaa
     1981 ggagattgga gtccgtaagg cgctcggggc tcggcgcaga gatattgtgc gtcagtttct
     2041 ctttgaggcg atgaccctaa ctttcctcgg tggcattctg ggtgtggttc tggctgttgg
     2101 cataagtaag ctgatcatgt tgctaatacc cagcttgccc gcttctattc ccacctgggc
     2161 ggtaatgtcc gggctgattg tgtctgttgg cgtcggattg atcttcggcg tctggcctgc
     2221 gcgtaaagcc tcgcgactcg atcccatcga gtgtctccgc tacgagtaaa cagtttcgaa
     2281 ttgggttttt gacgaactca gtttagttcg tgtcgttttg tgtgaatcgg tggttcgtgc
     2341 tgtgaaagac taaaacgaac cacgaacgaa cccgaatcgt cacgaaattt cttttcccac
     2401 cagttaactg caataactcc cccaagccag ctaatccatc tataatccaa ttctgaaagt
     2461 cttgaattcg tcacgagata ctgtgttgtg tttttgaccg ggccaccgaa aatgcccaaa
     2521 gctcaacgtg atttcatcaa agagtttctg acttacattc aggtcgagaa gggtcttgcc
     2581 cgtcacacgc tcgaaagtta cggacgcgat ctcgcgcggc tacaactgtg ggccgacaaa
     2641 gctggaaagc cgctcgagca gttgacgcgc gctgacctgc gaaagtggat tgccaacctc
     2701 tcgcgcgccg gactggcacc atcctccgtg gcacgcgcgg tcagcgctgc ccgcggcttg
     2761 tttcgcttcc tgatgctgga cggtcacatc aaacggcacc caacggaaga tctcgataca
     2821 ccacaacgct tcgcttacct cccgcagttt cttacggagg atgagatcaa ccagttactc
     2881 gccgcacccg atattgcaac tgcggaaggc gtgcgcgatc gcgcattaat tgaagtgatg
     2941 tacgctgcgg gattgcgagt atcggaattg atctcgctta agcaggccga tgtagatatg
     3001 caatctggtt tgttgatttg tcacggcaaa ggcagcaagg agcggcgcgt gccgattggt
     3061 aagagtgcta tccattggct gcagcagtac agcacggttc gcgccggtta tgggaaaccg
     3121 acatcacctc agttgttcct taaccgcggt aaacctatca cacgacaatt tgcctgggcg
     3181 ctgatcaagc gctatgcaag caaggccgga gtaaaagaca tttcaccgca cactctccgg
     3241 cacagtttcg caacgcat
//
LOCUS       DASUEX010000083         2456 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_477202, whole genome shotgun sequence.
ACCESSION   DASUEX010000083 DASUEX010000000
VERSION     DASUEX010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2456
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477202"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2456)
                     /locus_tag="VFV61_01440"
     CDS             complement(<1..>2456)
                     /locus_tag="VFV61_01440"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX4899162.1"
                     /translation="GCEFFGRKAGELVGVHLSELIGAEAGQHDTEQTRNQPRNQPVRS
                     RHWLANAQGQLRFLEGMITVEANNRGEPIAVRGVVRDLTEQKLAAEALKESEERYRRL
                     VECSPDAIVVTGEGKFVYVNSAAEALYRATSEQLIGKSILEIVHPDYHDFVTQRIRNI
                     QEDGRASNLTVQKHIRFDGEVIDVEVTGMPLTLQGKPAVQAVIRDVTDRTRAREALKE
                     TETRLQTVIGSASLVLFAVDKNGIFTLSEGEGLRALGLNPGDAVGQSVFDVYRDVPQI
                     LANIRRAMSGEAFSETVEVGELVFNTRYSPQYNQHGDVIGVIGVATDITETRKSEKAS
                     RENEERYRELVENANDIIYTHDLAGNFTSLNRSGERIVGYSWEEAAKMNISDVIAPEY
                     INLARQMITQKANSKTPTVYEIELVAKDGRRIRIEVSSRIIYESGKPVGVQGVARDLT
                     EHQRSQEALRESQTFFHSFMDNSPSIAFMKDAEGRYVYVNKPFEELFGQKLDFLRGKT
                     SFDWLPPVTAQNTHEHDLSVLTSGEPQEILETVPTQDGTPHHWLVVKFPMTDAAGKKF
                     VAGVGVDITERRQAEKALAKQAEREAITHGISQAVRRTLETSEIFESAVRELGSYLVV
                     DRCSLFMKDEKAQHLRNVAEYHAAGVKPAATDFALGDLTSLAQALSEKGVLVFNDAAN
                     DERIKTLYEKILKEADVQSIMYAAIRVGDEVPAAFALSTTRELRHWSDSDVALAKAVA
                     DQTGIAIRQAQLYQKAEATSTRESLVNRVSTALRASLSLPEVLRTATRELGQALSASR
                     VYLYLYHPENPVSVVEHEFVA"
BASE COUNT          525 a          709 c          630 g          592 t
ORIGIN      
        1 gcgacaaatt catgctcgac cactgacaca gggttctcgg ggtgataaag gtagagataa
       61 acccgtgacg cagataacgc ctgcccgagt tcccgcgtgg cagtgcgcaa aacttccggc
      121 agactgagtg aggcacgaag cgcggtcgag accctgttga ccaaagactc gcgcgtcgaa
      181 gtggcttcag ctttttgata aagctgcgcc tgacgtatcg ctattcccgt ttgatcagcg
      241 actgctttcg cgagcgcgac gtcggaatcg ctccaatggc gcagttcccg agtggtggac
      301 agcgcaaacg cagccggcac ttcatctccc acccggatcg ccgcgtacat aatcgactgc
      361 acgtctgctt ctttcagaat cttttcgtaa agggtcttga tgcgctcatc gttggcggca
      421 tcgttgaaca cgagaactcc tttttcactc agtgcctgcg caagactggt taagtcgccg
      481 agggcgaagt cggtcgccgc cggcttcaca cccgcagcat gatactcagc tacattcctc
      541 aggtgttgcg ccttctcgtc tttcatgaaa agcgagcagc gatccacgac gagataggaa
      601 cccaactcgc gcacggccga ttcgaagatt tcagatgtct ccagagtgcg ccgcacggct
      661 tgggaaatac cgtgagtaat tgcttcgcgc tctgcttgtt tggcaagcgc cttctcagcc
      721 tgtctacgct ctgttatgtc cacgccgaca ccggccacaa acttcttacc agcggcgtcc
      781 gtcatcggaa atttcaccac cagccagtgg tgcggtgtgc catcctgcgt gggcactgtc
      841 tccagaatct cctggggttc gccactggtc agcactgaca aatcgtgttc atgagtattc
      901 tgtgcggtaa ccggcggcaa ccaatcaaaa ctggttttcc cgcgcaggaa atcgagcttc
      961 tgcccaaaca actcctcaaa gggcttgtta acgtagacgt agcgtccttc cgcgtccttc
     1021 atgaacgcaa tgctgggact gttatccatg aagctgtgga aaaacgtctg actttcacgc
     1081 agtgcttcct gagatcgttg gtgctccgtt aagtcacgcg ctacaccctg gacgccaacc
     1141 ggttttccgc tttcgtagat gatgcgactg ctaacctcaa tgcgaatgcg ccgcccgtcc
     1201 ttagcaacta gttcaatttc atagacagtc ggagtcttac tatttgcctt ctgcgtgatc
     1261 atctgccggg ccaggttgat atattccggc gcaatcacgt cactgatgtt catctttgcg
     1321 gcttcttccc aactgtagcc aacaattcgc tcgccgctgc gattcaaaga agtaaaattc
     1381 ccggcaaggt cgtgggtgta gatgatgtcg ttagcgtttt caaccaactc gcgataacgc
     1441 tcctcattct cgcgagaagc cttttccgat tttcgggttt ccgtgatgtc tgtcgcaacg
     1501 ccgatgaccc cgataacatc gccatgctgg ttgtactgtg gtgagtagcg agtattgaaa
     1561 accagctctc caacttcgac cgtctcggaa aatgcctcgc cagacatcgc gcggcgaatg
     1621 ttcgccaaga tctggggaac gtctcgatac acgtcgaata ccgattggcc cactgcgtca
     1681 cccgggttga gtcccagtgc acgtaatccc tcgccctcag acagagtgaa aatcccattt
     1741 ttgtcgacgg cgaataacac gagtgaagcg gatccgataa ctgtttgcaa acgcgtctcg
     1801 gtttctttga gagcttcgcg agcgcgcgtg cggtcggtta catcgcgaat caccgcctgc
     1861 acggccggtt tgccctgaag cgtaagcggc atgccggtaa cttcgacatc gataacctcg
     1921 ccatcaaacc gaatgtgttt ctgcacggtc agattgctgg cccggccgtc ttcctgaata
     1981 tttcttatgc gctgggtgac gaaatcgtga tagtcaggat gaactatttc caggatggat
     2041 ttgccgatta gttgctcgct ggtggcgcga tagagcgctt cggctgctga gttgacgtaa
     2101 acaaacttcc cttcaccagt gacaacaatc gcatcaggcg aacactcgac cagtctgcga
     2161 tagcgttcct cactctcttt caacgcctcg gcagccagct tttgctccgt cagatctcga
     2221 acgacaccac gaactgcgat gggctcaccg cgattgtttg cttcgacggt gatcatgcct
     2281 tcgagaaagc gcagttggcc ttgcgcgttg gcaagccaat gacgcgaacg aacaggttga
     2341 ttgcggggct ggttccgagt ctgttcagta tcgtgctggc cggcttctgc gccaatcaat
     2401 tcactgagat gaacgccaac cagctctcca gctttgcgtc caaagaattc gcaccc
//
LOCUS       DASUEX010000084         2790 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1386629, whole genome shotgun sequence.
ACCESSION   DASUEX010000084 DASUEX010000000
VERSION     DASUEX010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2790
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1386629"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1168)
                     /locus_tag="VFV61_01445"
     CDS             complement(<1..1168)
                     /locus_tag="VFV61_01445"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX4899163.1"
                     /translation="MTRDTNYDVAIAGAGPAGTSAAIHLAEQGMKVLLAEQKKFPRAK
                     LCGEFISPECINHFERLGVAESIFAAKGATLIETSFYSRRGNKVTVPSHWFGSDRAAI
                     GLSRAEMDHLLLERAKRVGVTVLQEAQVVEPILKGQRVSGMRIKLNDQIDDYHAAITI
                     DATGRTRALARKLNATKDRRRARLVAFKSHLRNAQVAPGVCEIYFYPGGYGGLSNIEG
                     GFSNLCFITAARDVRRYGPDPQQVLKATVMRNSRAAFALAAANFEKPWLSVALESFGR
                     HQLVPAEGLLLIGDAAAFIDPFTGSGMLMALESGAVAAQTILSFRDRLNDESSFSQLS
                     NRFRANYTSTFNSRLRVSGWLRRAAFVPRLAEAAMICFGASDHLRRKVAQATRPS"
     gene            complement(1177..1953)
                     /locus_tag="VFV61_01450"
     CDS             complement(1177..1953)
                     /locus_tag="VFV61_01450"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX4899164.1"
                     /translation="MFSQFRQRSSELEHLDKGDYTPEEYEGCLVELRRINSWLGDTRA
                     LRETLLAEVEQLDLKRFSVLDVGAGSGELLRAAAHWAKHSQRTGKFVGLELNARSARA
                     IAEESTQFSEISALQADGFRLPFADNTFDYAICSLITHHFNDSAVVAFLRELGRTARR
                     SIFVIDLHRNPLAYYLYSTVGRVFLHNRLIREDGALSILRSFKPDELERLAQEAELED
                     IRIRQTFPARLVLSGRLQKPAGVARASHLNPHTQPVGTTA"
     gene            complement(2062..>2790)
                     /locus_tag="VFV61_01455"
     CDS             complement(2062..>2790)
                     /locus_tag="VFV61_01455"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HEX4899165.1"
                     /translation="AYAAEHIALGRQNVMIAGGVDAPIAPGILAAFNLMTVLTSEWNE
                     EPQRASRPFSRNRSGIVLGEGAWLYVLEDLEHAQARDAKIYAEIIGYGATCDAYHRVR
                     LEEGGDEPARAMRLALEDAGRRPEDVDYVNLHGTSTLLNDRIETSALKLAFDGHAKRI
                     PMSATKSQVGHPQGASGAAGLGAALCAMHEKMIPPTINLDEPDPDCDLDYVANNARAA
                     DVRIALCNCIGFGSKNSALLVARM"
BASE COUNT          606 a          805 c          736 g          643 t
ORIGIN      
        1 acgatggtcg cgttgcttga gccactttgc ggcgtaaatg atcgctggct ccaaaacaga
       61 tcattgcggc ctcggcaaga cgaggtacaa aggcagcacg acgcagccaa ccgctcaccc
      121 gcaaacgtga gttgaaggtg ctggtgtaat ttgcacggaa acgatttgac agttgcgaaa
      181 acgaactctc gtcgttgagt cgatcgcgaa agctcaagat tgtctgcgca gcaacggccc
      241 cgctctcgag tgccatcagc attccgctgc cggtgaaggg atcgataaac gcggccgcgt
      301 caccaatcaa cagcaggccc tcagctggta ccaactgatg ccggccaaaa ctctccaaag
      361 cgacacttaa ccacggcttt tcaaagttcg ctgccgcaag cgcaaacgct gctcgtgaat
      421 ttcgcatcac cgtcgctttc agaacttgct gcggatccgg accataacgc cgcacgtcac
      481 gagctgccgt gataaagcaa aggttgctaa aacctccttc aatgttgctc aaaccaccat
      541 agccaccggg gtaaaagtag atttcacaaa ccccgggggc tacctgggcg tttcgtaggt
      601 gcgacttaaa ggctactaat cttgcccggc gccgatcctt tgtagcgttg agtttccgcg
      661 cgagggctcg tgtccttccg gtcgcatcaa tcgttatggc ggcgtgataa tcgtcgatct
      721 gatcattcag cttgatgcgc attcctgaaa cccgctgccc cttgagaatt ggttcgacaa
      781 cctgcgcttc ctgcaaaacc gttacgccta ctcgcttcgc tctttcgagc agcagatgat
      841 ccatttcggc acggctgaga ccgatggccg cacggtcgct tccgaaccaa tggctcggaa
      901 ccgttacttt gtttccccgc cgcgagtaga aactcgtctc aattagcgtc gcgcctttcg
      961 ctgcaaagat gctttcggct acgccaagcc gctcgaaatg attgatgcac tccggggaaa
     1021 taaattcacc gcaaagtttg gcgcgcggaa acttcttctg ctccgctagt aaaaccttca
     1081 ttccctgctc agccagatga attgccgcgc tcgttcccgc cggtccagcg ccggctattg
     1141 ctacgtcgta attggtgtcc cttgtcatcg ccactgttac gcagttgtgc cgactggttg
     1201 tgtgtgaggg tttaagtgag acgcgcgggc aacgccggcg ggcttctgca aacgaccgct
     1261 aagcaccagg cgagcgggaa acgtctgcct tatcctgata tcttccaact cagcttcttg
     1321 tgcaagtcgc tccaactcgt cgggtttgaa actgcgcaag attgaaagtg cgccgtcttc
     1381 acgtatcagg cgattgtgaa gaaatacccg gcccactgtg ctgtacaaat agtacgcgag
     1441 gggatttcta tgaagatcga tcacgaagat ggagcgccgt gccgtgcggc ctagctctct
     1501 cagaaaagcc acgaccgcgg aatcgttaaa gtgatgagtg atgagcgaac aaatcgcata
     1561 gtcgaacgtg ttgtctgcaa atggcagccg gaagccgtct gcttgaagag cgctaatctc
     1621 agaaaactga gtagattctt ctgcgattgc tcgagctgag cgcgcattga gttcaagacc
     1681 aacaaacttg ccggtccgtt gcgagtgttt tgcccagtgt gccgcagcgc gtagcagttc
     1741 gcctgaaccg gcgccgacgt cgagaactga aaagcgcttc agatctagct gctcgacttc
     1801 cgctagaaga gtctcccgca gggctcttgt gtcgccgagc caggagttga tgcggcgcag
     1861 ttcgaccaaa cagccctcat attcttccgg cgtatagtcg cccttatcga ggtgttctag
     1921 ttcactgctg cgttggcgaa attgcgaaaa catgtaactg ggtgactgtc tggcgggtgt
     1981 cgggtgacag cgtcaggttt cgggtttcaa gcgttcgtcg cccgtcacct gatccccgac
     2041 acctgatccc catcaccggt ttcacatcct tgcaactaac aacgcgctgt tcttggaacc
     2101 gaaaccgatg cagttgcaaa gcgcgattct tacgtcggcc gcacgtgcgt tgttagccac
     2161 ataatccaga tcgcagtccg gatcgggctc atcaaggtta atcgttggcg gaatcatctt
     2221 ctcgtgcatc gcacagagcg ctgctcctaa cccggctgca ccgctggctc cctgcggatg
     2281 acccacttgg gacttagttg ctgacattgg aatgcgttta gcgtggccat caaacgccaa
     2341 tttcagcgcg ctcgtttcga tacggtcgtt tagcagtgtc gatgtcccat gcagattcac
     2401 gtaatccacg tcttccggac ggcggccagc atcctctaat gcgaggcgca ttgctcgcgc
     2461 tggctcatcg ccaccttcct cgagccgtac gcgatggtag gcatcgcaag tagcaccgta
     2521 gccgattatt tcggcgtaga tcttcgcgtc acgtgcctgg gcgtgttcta gatcttcgag
     2581 aacgtagagc caggcgccct ctcccaaaac aatgccggat cgattgcgtg agaatggtcg
     2641 cgacgctcgc tgcggctctt cattccattc actggtgagt acagtcatca ggttgaacgc
     2701 cgcaagaatt cctggagcaa tcggtgcatc aacaccgcca gcaatcatta cattctgacg
     2761 tcccagcgca atgtgttctg ctgcataagc
//
LOCUS       DASUEX010000085         2793 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_820818, whole genome shotgun sequence.
ACCESSION   DASUEX010000085 DASUEX010000000
VERSION     DASUEX010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2793
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_820818"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="VFV61_01460"
     CDS             <1..442
                     /locus_tag="VFV61_01460"
                     /inference="COORDINATES: protein motif:HMM:NF013430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899166.1"
                     /translation="RADLAIGAVLVHGARAPFVVRREQLALMKPGAVLVDVAIDQGGC
                     IETTHPTTHSDPTYYVEGVLHYCVANMPGAVPRTSTFALTNATLPYALKLANKGFLNA
                     IGSDNGLKEGVNTYDGYCTYEAVAVSQDLQYTALDTLISKSVNA"
     gene            complement(533..967)
                     /locus_tag="VFV61_01465"
     CDS             complement(533..967)
                     /locus_tag="VFV61_01465"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX4899167.1"
                     /translation="MSTHEELFSTPVSQNSPSDHLSGREITVLLVEDDRSLRRFLEVV
                     LERAGYRVKSAGDGVEGMKAALSLNIDIVITDAIMPNLNGYELCRFLRETPGLSQIPI
                     VLLSALEPVGSQRNEPIDAFLVKPVAPEGLLECLAGLLVKVR"
     gene            complement(1071..>2793)
                     /locus_tag="VFV61_01470"
     CDS             complement(1071..>2793)
                     /locus_tag="VFV61_01470"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bi-domain-containing oxidoreductase"
                     /protein_id="HEX4899168.1"
                     /translation="ELGLGEAVVVIGLGLLGQITVQLLKANGCRVFGVDLDLLKVQLA
                     RDLGADAASVTGPDTRDQILEWSRGRGADAVLITAATSSNEPLELAGDVSRRKGRVVA
                     VGLVGLDVPRQAFYERELTLKISMSYGPGRYDPDYEERGHDYPFAYVRWTEQRNIEAF
                     LDLLAEGRINVDRLITHRFPIQEAERAYELLSGETNTSYLGVILQYEPERELTPLVPL
                     RQRRAIAAEKKAESVELGLIGAGKYVQSFLLPQFKFAGVNFRSISSASGVSAQNVGAK
                     FGFASAVSSADEIIADPEINLVVVGTHHDTHARLAVSALQNHKDVFVEKPLALNDEEL
                     EGVLKAALDSRGRLMVGFNRRFAPLSRKAREVFSERRSPLSILYRVNAGRVPKGHWTQ
                     DREEGGGRIIGEVCHFIDLMQFWVGSPPVSVFAESISGQNDEIVNDDSVFITLKFADG
                     SNGCIAYLAEGNKKLPKEHIEIFGGGKSFVIEDFREAVLFKNGREEKMRLRAQDKGQA
                     EQVKAVCQVVLGKGLPPISLEELEATTRTTFRINDSLRLGQPMTVAGAEWPWAFNNNG
                     PETAGKP"
BASE COUNT          629 a          824 c          698 g          642 t
ORIGIN      
        1 ccgcgccgat ctggcgatcg gcgccgtgct ggttcacggc gcgcgcgccc ccttcgtcgt
       61 ccgccgtgag cagctcgcgc tgatgaagcc cggcgcggtg ctggtcgacg tcgcgatcga
      121 ccagggcggt tgcatcgaaa caactcaccc gacaacgcac tccgatccga cctactacgt
      181 tgaaggagtg cttcactact gcgtcgccaa catgcccggc gcggttccgc gcacgtcaac
      241 attcgccctg accaacgcca cgcttccgta cgctctaaaa ctcgcaaata aggggttcct
      301 taacgcgatt ggtagcgata acggattgaa ggaaggggtc aacacctatg acggatattg
      361 tacttatgag gctgtcgctg tctcacaaga tctccagtac accgcactcg acacattgat
      421 cagcaaaagc gtcaacgcct gaattccggt ctgcgacaca aaataacgcg gcgttccttt
      481 acggtccgcc gcgttattac ttgcccccct tgaaagacgt ctgctcgtag ttttacctga
      541 ctttcaccaa cagccccgcg aggcactcca gcaacccttc aggtgctact ggcttgacca
      601 ggaaagcatc gatgggttca tttctttgac tgccgacggg ttcgagggcg ctgagcagaa
      661 cgattggtat ctgcgaaagg cccggcgtct cgcgaagaaa gcggcagagc tcatagccat
      721 tcaggttcgg catgattgcg tcagtaatca cgatatcaat attcaagctc agggcagcct
      781 tcattccttc aacaccatct cctgcagatt tgacgcgata cccggcgcgc tcaagcacga
      841 cttccagaaa cctcctgagc gagcgatcat cttccaccag caagacagta atctccctgc
      901 cgctcaggtg gtcgctgggc gaattttgtg acactggggt tgaaaatagt tcttcgtgag
      961 tgctcatcat gaatggctgg attttctgga ggtaatgagg cggtttaggc gtgccctaga
     1021 aggccagcag gcataagcag agtaaggctg cggacggagg gttgtcaata ctatggtttg
     1081 ccagcggttt ctggcccatt attgttgaat gcccagggcc actcagcgcc agcgacagtc
     1141 atcggttgcc ccaatctcaa cgaatcattg attcgaaacg tggtgcgtgt tgtggcctcc
     1201 agttcctcca atgagatcgg aggaaggccc tttcccaaga ccacctgaca gactgctttt
     1261 acctgctctg cctggccttt gtcttgagcc ctcaacctca ttttttcttc gcgcccattt
     1321 ttgaagagca ctgcctcgcg aaagtcctcg atgacgaatg actttccccc accaaaaatc
     1381 tcgatatgtt cctttggcag cttcttattg ccctcggcta agtaagcgat gcagccattg
     1441 gatccgtcgg caaatttgag cgtaataaag acggaatcat cgttcacgat ctcgtcgttc
     1501 tgtcccgata ttgattcggc gaagactgag acgggtggtg agcctaccca gaactgcatt
     1561 agatcaatga aatgacaaac ctcgccaata attctgccac ctccttcctc gcggtcttga
     1621 gtccaatgcc ctttcgggac gcgtccagca ttcacgcgat aaaggatcga cagcggtgag
     1681 cgtcgctcag aaaagacttc cctggctttc cggctgagag gagcaaacct gcggttaaag
     1741 ccgaccatga gccggcctct ggaatccagc gcagccttaa gcacgccttc caactcctca
     1801 tcattaaggg cgaggggttt ttccacgaag acgtctttat ggttttggag agcacttact
     1861 gccaatcgcg cgtgcgtgtc atgatgcgtg cctacgacaa ccagattgat ctcggggtcg
     1921 gcgataatct catctgcact agaaactgcg ctcgcaaagc caaactttgc tcccacattc
     1981 tgggccgaaa ctccggaagc gctgctgatc gagcgaaagt tgactcctgc gaacttgaat
     2041 tgaggtaaca ggaatgactg gacgtatttc cctgcgccaa tcaaccccaa ctccacagac
     2101 tctgcctttt tttctgcggc gattgctctg cgctgtctca gcggaactaa cggtgtaagc
     2161 tccctttcgg gttcatattg caggatcaca ccgaggtaag aagtgttagt ctcgccgctg
     2221 agcagttcgt aagcgcgctc ggcttcctga atgggaaagc ggtgcgtgat caagcgatct
     2281 acattgattc gcccttcggc gagcagatca aggaacgctt caatattccg ctgctccgtc
     2341 caacgaacat aggcaaacgg gtaatcgtga ccgcgttctt cgtagtcggg atcatagcgt
     2401 cccggcccat atgacattga gatcttcagc gtcaattctc tttcatagaa tgcttgccgc
     2461 ggcacgtcca atcccaccag tccaactgca accacgcgac ccttgcgccg tgacacatca
     2521 cccgccagct ccaggggttc gttcgaagag gttgctgccg tgatcagaac tgcatccgcc
     2581 ccacgcccac gactccattc gagaatttga tctctggtgt ctggacccgt aaccgaggct
     2641 gcgtcggcgc caagatcacg ggccagttgt actttgagca aatccaagtc gacgccaaat
     2701 actcgacaac cattggcttt caatagctgc actgtgattt ggcccagcaa accgagcccg
     2761 atcaccacta ccgcctcacc taaacccaac tcg
//
LOCUS       DASUEX010000086         4030 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_477426, whole genome shotgun sequence.
ACCESSION   DASUEX010000086 DASUEX010000000
VERSION     DASUEX010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4030
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477426"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /locus_tag="VFV61_01475"
     CDS             <1..591
                     /locus_tag="VFV61_01475"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX4899169.1"
                     /translation="SLAPEDAAKRETFLRLADQLKAEVVRINRHITDFLKYSRPSTLD
                     FQQVDLRTEAEDALRLIEAQAAEHGVKTSVEVEPSLPAIVADRETLRSAFTNLIINGL
                     QAIDGDGGNIVIKLSSADSKRVRVEVTDTGRGIAPEDISKVFEPYYSTKDTGTGLGLA
                     IVRKAVEDHGGTVSVHSTQGSGTTFTIILPVKPQDG"
     gene            708..2054
                     /locus_tag="VFV61_01480"
     CDS             708..2054
                     /locus_tag="VFV61_01480"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX4899170.1"
                     /translation="MSRKSILVVDDEKSQREILEMILTSEGYDVTTASSGEAAIKFAK
                     DRRFDLALTDLKMTGMDGIELLQHLLALDSSIIVILLTAHGSIESAKEALRRGAFDYM
                     EKPYDKDALLATINRALDKLDAIDVEIISASPKMEAVKRMIVKVARSNSTVLIRGESG
                     TGKELIARSIHNQSPRAQEMFQAVNCAAINENLLESELFGHEKGSFTGAHADKKGLFE
                     IADRGSLFLDEIAELDVAMQAKLLRALQERKIRRVGGTHEIGVDVRVIAATNRDLRAM
                     VADGRFRDDLYYRINVLSVDVPPLRERRDDIPVLIDFFLKKHTRNTSRLVRGLTAETR
                     KVMHEYSWPGNVRQLESAIERAILLCEGDLITIDDLPLEVRQEARPAAEGAFKLPAEG
                     INFEEVERDLLTQAMEQTDYNITKAAKLLGFTFRTLQYRLEKFGIKKPEDKKEAAG"
     gene            2212..2736
                     /locus_tag="VFV61_01485"
     CDS             2212..2736
                     /locus_tag="VFV61_01485"
                     /inference="COORDINATES: protein motif:HMM:NF022853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD
                     domain-containing protein"
                     /protein_id="HEX4899171.1"
                     /translation="MIRLIAVLSLALMLVGGAACSRTAQNGSTPSPSPEPTRITSESL
                     VKVTAEPMEVAGGQSNEAVVKITTQSGYHINANPASFDYLKATEVDLPDTPEVTVDYI
                     YYPNPQVKKFSFSDQAIRVYEGETQLRVMLMADKAAPKGARSLPAKLNVQACDDKVCY
                     PPGAVNFTIPVTIK"
     gene            2733..2945
                     /locus_tag="VFV61_01490"
     CDS             2733..2945
                     /locus_tag="VFV61_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899172.1"
                     /translation="MKTLWLALAGCCIAAAAVLMALGHFDGAFVVAVIGMIAWFLNYR
                     AQVRKSLDAEDATTEEEIETERNEDQ"
     gene            2932..3459
                     /locus_tag="VFV61_01495"
     CDS             2932..3459
                     /locus_tag="VFV61_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899173.1"
                     /translation="MKINKRVVFGVLLFTFMTVLPAQAADEAAVRGTVQRVFQQLKSR
                     EYSALYDSLPSSSRARMTRERFASSLRRAQDMYVLDRIDVGQVRLAKDIAVVDTVLYG
                     RVVAPVAVEGKIVVQQYLVREDGQWRVATGDSGTIKRFLANNPTFRAKFPIRQPRVFV
                     RQGNNWVEFSPGKRR"
     gene            complement(3606..3857)
                     /locus_tag="VFV61_01500"
     CDS             complement(3606..3857)
                     /locus_tag="VFV61_01500"
                     /inference="COORDINATES: protein motif:HMM:TIGR01764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX4899174.1"
                     /translation="MEEREEFLTARQLAKVLQVSESTVRRLARKRRIPSVRVTPRIIR
                     FHLDAVRESLDVPKHRIRHGEYTETVDDGQLSFEDLLLS"
BASE COUNT          996 a         1037 c         1141 g          856 t
ORIGIN      
        1 tcgctcgcac cggaagatgc tgctaaacgc gagacatttc tccgtctcgc cgatcagctc
       61 aaggctgagg tggtgcgcat taaccgtcac ataactgatt tcttgaagta ttcgcgcccg
      121 tcaacgctcg atttccagca ggttgactta cgaaccgaag cagaagacgc acttcgcttg
      181 atcgaagcgc aggccgctga gcacggcgtg aaaacgagtg tggaagtgga gccatcattg
      241 ccggcaatcg ttgcggaccg ggagactctg cgatccgcgt tcacgaacct gataatcaac
      301 gggcttcagg cgattgacgg agatggtggt aacatcgtca tcaaactttc gagcgcagac
      361 agcaaacgag tccgagtaga ggtgactgat accgggaggg gtattgctcc cgaggatatc
      421 tcgaaagtct ttgagcctta ctactcaacc aaagacacag ggacgggtct gggtctggcg
      481 attgtcagaa aagcggtgga ggaccatggc gggaccgtca gtgtacattc gacacaaggg
      541 agcggaacta cttttacgat catattgccg gtgaagcccc aagacggtta aaggactgga
      601 gatgcggtga cgcggtgacg cggagacacg gagccgctgc gatgggggtg aaaacgctcc
      661 atcgttcacg atgaccttaa tagtcagttt cttttgagac tcaaaacatg tcgcgcaaaa
      721 gcatattggt agtagacgac gaaaagtcgc aacgagaaat tctggaaatg atcctgacga
      781 gcgaaggcta cgacgtcacc accgcgtcgt cgggagaagc ggcaatcaag tttgccaagg
      841 atcgccgatt cgatttggcc ttgaccgatc tgaagatgac aggcatggac ggcatcgagt
      901 tgctgcaaca cctgctggct ctcgattcct cgattatcgt gatcctcctg acggcgcacg
      961 gatcgatcga gtcagccaag gaagcactgc ggcgcggcgc ttttgactac atggagaagc
     1021 cgtacgacaa ggatgcgctg ctggcaacca tcaatcgcgc gctcgacaaa cttgatgcca
     1081 tcgacgttga gatcatttcc gcatcgccaa aaatggaagc cgtgaagcgg atgatcgtca
     1141 aagtcgctcg ctcgaattcc acggtgctaa tccggggcga gtcgggcact ggtaaggagc
     1201 ttattgcgcg ctcgattcat aatcagtcgc cgcgcgccca agagatgttt caggcggtca
     1261 attgcgcggc aattaacgag aacctgttgg agtcggagct ctttgggcat gagaagggct
     1321 cgttcacagg agcacacgcg gataagaagg gattgtttga gattgccgat cggggttcgt
     1381 tatttctcga cgaaatagcc gagctcgacg ttgccatgca agcgaagctc ttgcgcgccc
     1441 tgcaagagcg aaagatccgg cgcgtcggcg gcacgcatga aatcggcgtg gacgtgcgtg
     1501 tcattgccgc aaccaaccgc gacctgcgag caatggtcgc cgatgggcga ttccgcgacg
     1561 atctctacta ccgcatcaat gtgctgtcgg tagacgtccc gccgttgcgc gaacgtcgcg
     1621 acgacattcc cgtactgatc gatttcttct tgaagaagca cacccgtaac acctcccggt
     1681 tggtccgtgg tcttaccgca gaaaccagaa aggtgatgca cgagtacagt tggccaggta
     1741 atgtgcggca gttggagtcg gcaatcgaac gggcgattct gctctgcgaa ggcgacctga
     1801 tcacaattga cgacttaccc ctggaagttc gtcaggaagc gcgaccagcc gctgaaggcg
     1861 ctttcaagtt gccggcggaa ggcatcaatt ttgaagaagt tgagcgcgac ctcctgacgc
     1921 aggcgatgga acagaccgat tacaacatca caaaggccgc gaagctcttg ggcttcacct
     1981 ttcgtacttt gcaatatcgt ctggagaagt ttggcattaa gaaaccggaa gacaaaaagg
     2041 aagcggccgg atagggattg cctattgatg attgccgatt ggcgattgcc gatttgccct
     2101 tcgctaaacg gtcagttgga gctaggcaat tgacaattat ccaatcccgc cagattggcc
     2161 ggtcacagcg gcaatcggca atccgcaatc tgctttaggg gcacacagaa catgatcagg
     2221 ttaattgctg ttttgtcact cgcgttgatg ctggtcggcg gagcagcatg ctcgcgcacc
     2281 gctcagaatg gcagcacacc ctcaccttcg ccagagccaa ccaggatcac ttcagaatcg
     2341 ttagtgaaag tcacggcaga accaatggaa gtggcaggcg gacaatcgaa tgaggccgtg
     2401 gtcaagatca ctactcaaag cggttatcac ataaatgcaa acccggcgag tttcgactac
     2461 ttaaaggcca ccgaggttga tctgcctgac acccccgaag tgaccgtcga ttacatctac
     2521 taccccaatc cacaggtaaa gaagttttcg ttttctgacc aggcaatcag ggtttatgaa
     2581 ggtgagacac aattgcgagt gatgctgatg gccgacaagg cagctcctaa aggcgctcgc
     2641 agcctgcctg ctaaactcaa cgttcaggct tgcgacgaca aggtttgtta cccaccaggt
     2701 gcggtcaatt tcacaatccc ggtaacgatc aagtgaagac actctggctg gcgctcgctg
     2761 gttgttgtat cgccgcggca gccgtgctga tggcacttgg ccattttgac ggcgcattcg
     2821 tcgtggccgt gataggcatg atcgcctggt ttctaaatta ccgggcacag gtaaggaaaa
     2881 gtctggacgc tgaggatgca accacagaag aggaaattga aactgaaaga aatgaagatc
     2941 aataagcgag tcgtattcgg ggttctcctt ttcacattca tgacggtgct gccggctcag
     3001 gcggcggacg aagcggcagt gcgcggcacc gtgcagcgag tctttcaaca actgaagtcg
     3061 cgcgaataca gcgcgcttta cgattccctt ccttcgtctt cgcgcgcgcg gatgacacgc
     3121 gaacgctttg cgagctcctt gagacgtgcg caagacatgt acgttctcga tcgcattgac
     3181 gtcggtcagg tacgtctggc aaaagacatt gccgtagtcg atacagttct gtatgggcga
     3241 gtggtggcgc cagtggcagt ggaaggaaag atcgtggtgc aacagtatct ggtgcgggaa
     3301 gatggccagt ggcgagttgc taccggcgat tcgggaacga tcaaacgctt cctcgcgaac
     3361 aatccgacat tccgcgcgaa gtttccgatt cgccagccgc gggtttttgt gaggcaggga
     3421 aataactggg tggaattctc gccgggtaaa cgacggtgag gtcgcagctc aaaaacgcag
     3481 agctgctgag acacaaagcc gcagagacca ttctgcgagc acgctcagcc aactaaccgt
     3541 aactttcttg aattctcttt gcgactttgc gtctctgcgc ctcagcgttc aggctaacct
     3601 caaaatcaac tcaacagcag atcttcaaat gagagttggc cgtcgtccac cgtctcggtg
     3661 tactcgccat gacgaatgcg gtgcttggga acatccagcg actcacgaac cgcatcgaga
     3721 tggaagcgga tgattctagg tgtgacgcgc actgaaggaa tgcggcgctt gcgggccagt
     3781 cgccgcacag ttgactcgct gacttgcaat accttcgcaa gctgacgagc ggtcaggaat
     3841 tcctcacgct cttccatggt tgggtagcaa tatcctaacg caggaaatca aaccgatcca
     3901 aacataaaac cgggcaaaaa ttggcgattg ccaattgcca atattgccaa ttgaatattt
     3961 ttgacccaaa cgtatttgcg gattggtaat cgccaatcag taatcgcctc agcaattggc
     4021 atttggcaat
//
LOCUS       DASUEX010000087         2871 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1613356, whole genome shotgun sequence.
ACCESSION   DASUEX010000087 DASUEX010000000
VERSION     DASUEX010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2871
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1613356"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..532)
                     /locus_tag="VFV61_01505"
     CDS             complement(<1..532)
                     /locus_tag="VFV61_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899175.1"
                     /translation="MKKTAPYGSWKSPITADLIVSGAVGLSQPAIDGADIYWVELRPA
                     ESGRQVIVKRNQQGELSDINPQPLNARTRVHEYGGGDYLVSNGKVYFSNFADQRLYKQ
                     VAGEAPQPLTPAAEMRYAEAIFDGARDRLICVREDHSDPGREAINALVSLSASGNHGC
                     GEILVTGNDFYSTPQLS"
     gene            complement(734..>2871)
                     /locus_tag="VFV61_01510"
     CDS             complement(734..>2871)
                     /locus_tag="VFV61_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899176.1"
                     /translation="WNKVLFVNDTTGFIDLQADPKRPDTLYAAAWQRFRFGGGDMAES
                     GPGSGLYKSVNGGKTWQRLTNGLPKEDMGKITVAVARGNSNIVYAAILTGEPAPGGKR
                     TIDTGGIFRSQDGGRNWQRMNSAMTSYYYDRINVDPVDDNRTWMPVFDLMVSTDGGKT
                     LVKANMKHVHNDQHGLWINPSDPQHLIMGGDGGVNISWNRGATWQQAVLPIGQFYEVA
                     VDNQDPYYVYGGMQDTGHWVGPSQTYDNEGITNHDWIKLRFNGDGMPIHTDPRDPNIV
                     YMVQEFGNFSRLDLRTWDRAELLPPPEEAQRRGLHSFRYDWTPPMIISQHDPNVLYLG
                     SNYLFRFTERGDKWDVISPDLSAQQEKTLKGSKTDYTGYHSYGALFSIAESPLDAKII
                     WTGADDGPVHLTKDGGKSWEKVSRNFPKSAPTFAVVGEIEASRFDAAAAYVVYDNHTR
                     EDHRPYVYRTADYGKTWHDITSDLPTNGSSYVIREDPINPNLLFLGTEFGVFVTIDRG
                     NHWVQLKNNLPTVAVRTMAIQARDRDLIVGTFGRAIWIMDIGPLEELSTSVFEQPGYV
                     FDVKPGTLFKTRFTYGATIEELNGDMFFRAENPPFGTVISYYVPGNLGTEVSISIQDE
                     KGNVVRTLAGPGTAGIHRVNWDLKRQTRVTDAEATRAGVTTISEREALDWVAPGQYTA
                     ILAIGPTSVRNTILVRKETAGVRVGSVRK"
BASE COUNT          632 a          795 c          738 g          706 t
ORIGIN      
        1 gactcaactg cggtgtggaa tagaagtcat tgccggttac gagtatctcg ccgcaaccat
       61 gattcccact tgcactcagg cttaccaagg cattgattgc ttcgcgaccg ggatctgaat
      121 gatcctcgcg cacgcagata aggcgatcac gagcgccgtc gaatatcgct tccgcgtaac
      181 gcatctccgc ggcaggcgta agcggttgtg gcgcttcacc ggcaacttgc ttgtagagtc
      241 gttgatcggc aaagtttgaa aaatagactt tgccattgct aaccaaataa tctccgccac
      301 cgtattcgtg aacccgtgtc cgcgcattaa gcggttgtgg gttgatgtca ctgagttcgc
      361 cttgttggtt gcgtttcaca atgacttgac ggccactttc tgctggccgc aactcaaccc
      421 aataaatgtc agcaccatcg atcgccggct gactcaggcc cacagcgcca ctaacgataa
      481 gatcggcagt aattggtgat ttccacgagc cataaggtgc tgtctttttc atcgggtttt
      541 gtttgatttg atggtccgtc ttagatttcg cgattctttt gttacattct cgtaaagcct
      601 ttgcaaggag gctgcaacct accggtccag gcttgggtct aacctgcgcg catcgttaat
      661 tctccatggg agaactgaga taaaggcaag ggcgtcggtc ttgggggagg tcgacgccct
      721 ctctttttgc cttttacttc cttaccgaac cgactcgcac acccgctgtt tcttttcgca
      781 ccaatatcgt gtttctcact gaagttggtc caattgccaa gatagctgtg tattgtccgg
      841 gagccaccca atcgagcgcc tcgcgttcgg aaattgtggt gacaccagct cgagtggcct
      901 cggcgtcggt gactcttgtt tgccgcttga gatcccaatt cacccggtga atgcccgctg
      961 tgcccgggcc tgcaagcgta cgaacgacat ttcctttttc gtcttgaatg ctgattgaaa
     1021 cctcggtccc cagattcccg ggaacgtagt aactgatgac ggtgccgaag ggcggattct
     1081 cagcgcgaaa gaacatatcc ccattcaatt cctcgatcgt cgcgccataa gtgaaacgag
     1141 tcttaaagag ggttccgggt ttaacgtcga agacataacc cggttgctca aatacgctcg
     1201 tgctcaactc ctcgagaggg ccgatgtcca tgatccaaat cgcgcgacca aatgtaccga
     1261 cgatcaaatc gcggtcgcgc gcttgaatgg ccatcgtccg aacagcaacg gtgggaagat
     1321 tgttcttcag ttgtacccag tggttgccgc gatcaatagt tacgaaaact ccaaactccg
     1381 tacccaggaa caagaggttg ggattaatgg gatcttctct aatcacataa cttgaaccgt
     1441 tcgtgggtaa gtcgctggtg atgtcatgcc acgttttccc gtagtccgct gtgcgataca
     1501 cataaggtcg atgatcttcg cgcgtgtgat tgtcataaac gacataagcg gcggccgcgt
     1561 cgaaacgcga agcctcaatc tcaccaacga cagcgaaggt gggagcgctc ttgggaaaat
     1621 tgcgactgac tttttcccag ctcttgcccc catctttcgt aagatgaacc ggcccgtcgt
     1681 cggctccggt ccagattatc ttcgcgtcta agggcgactc ggcgattgag aagagcgcac
     1741 cgtaactgtg gtacccggtg tagtcagttt tgcttccttt gagggtcttc tcctgttgcg
     1801 cacttaagtc cgggctaatc acgtcccatt tatcgccacg ttctgtgaaa cgaaagagat
     1861 aattgcttcc gaggtacagc acgtttggat cgtgctggga aatgatcatc ggcggggtcc
     1921 aatcgtagcg aaaactgtgc agcccacgtc gctgtgcctc ctcaggtggc ggcaggagtt
     1981 cggcacggtc ccaggtccgc aaatccagtc gcgagaaatt accaaactcc tgaaccatat
     2041 agacgatgtt aggatcgcgc ggatcggtgt gaatgggcat cccgtcgcca ttaaagcgaa
     2101 gtttgatcca atcgtgatta gtaattccct cgttgtcgta ggtctgactc ggcccaaccc
     2161 agtgacccgt atcctgcatg ccaccgtaga cgtagtaagg atcttgattg tctacggcca
     2221 cctcatagaa ctgtccaatc ggcaagacgg cctgctgcca ggtggcgcct cgattccatg
     2281 aaatattcac accgccatcg ccacccatga tcagatgttg cggatcagag ggattgatcc
     2341 agagtccgtg ctgatcattg tgcacgtgct tcatgttggc tttcaccagc gttttgccac
     2401 cgtcggtcga caccatcaaa tcaaacaccg gcatccaagt gcgattgtca tcaacagggt
     2461 caacgttgat gcgatcgtag taataactag tcatggcaga attcatgcgc tgccaattgc
     2521 gaccgccatc ctgcgaacga aagatgccgc cggtatcaat tgtccgcttg ccacccggag
     2581 cgggctcgcc agtcaatatc gccgcataga ctatgttgct gttcccgcgc gcaacggcca
     2641 cagtgatctt gcccatgtcc tctttcggca aaccgtttgt cagtctttgc caggtcttcc
     2701 caccgttgac cgacttataa aggccactgc caggaccgga ctcggccatg tcgccgccgc
     2761 caaaacgaaa gcgctgccag gctgcggcgt aaagcgtgtc gggacgtttc ggatcggcct
     2821 gcagatcaat gaagcccgtc gtgtcgttta cgaacagaac cttgttccag c
//
LOCUS       DASUEX010000088         2647 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_706874, whole genome shotgun sequence.
ACCESSION   DASUEX010000088 DASUEX010000000
VERSION     DASUEX010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2647
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_706874"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="VFV61_01515"
     CDS             <1..251
                     /locus_tag="VFV61_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899177.1"
                     /translation="DDATLIYPGAKTIVDFSSGDGRAMQLQTGDSLEQVIAWYEKSIK
                     PTKTMRLTSTSVVLKNENVTTTLASEGGKTNILIKRVK"
     gene            407..1111
                     /locus_tag="VFV61_01520"
     CDS             407..1111
                     /locus_tag="VFV61_01520"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEX4899178.1"
                     /translation="MTLFVGTSGYSYKEWKGSFYPEKLPAKEMLAYYATKLPAVEINN
                     TFYRLPQKSVLENWKEQVPESFRFSVKASQRITHFKRLNGVSDETSYLLETAAALGDC
                     LGPILFQLPPNMKKDLARLETFLSTLPAGTRAAWEFRHPTWLEDDVLELLSRHNHALC
                     ISDTDQMPVSHVDKTADWGYLRLRRVSYSAENLAEWLQRVRDQNWEFAYVFFKHEDEG
                     TGPRLAGDFLKLAGAG"
     gene            complement(1433..>2647)
                     /locus_tag="VFV61_01525"
     CDS             complement(1433..>2647)
                     /locus_tag="VFV61_01525"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEX4899179.1"
                     /translation="TPKRVRRLVAKSRGRLCVRYQSLPDGSILLKAIPKKVHQIQRRV
                     SRIAAGAFSATLTLTNAMAQPGADRLGYEHTFSYENGASVEVIALSATLKGRVIDPHG
                     AVIQGAGITLGNDEAPYLLGAISDSLGEYKLEGLQPGNYRLTITATGFANHEIHGLAI
                     TANETQTIESKLEIAVIQYELEVVANEQQSEQLEVLSGAVAIQAEQPLIRAANDDDLQ
                     DLERLLTRDNVNVRDKNTGRTALEHAVLNNNREMVQVLLAAGADVNSRDASKQTALML
                     LGSETTVEIVWDLINAGAKVNLRDEEGDTALTEIASEKNLPALMALLHAGAKVEDKND
                     EGQTALIIAARSEQVANVRALVRAGADMNARDKKGWTALDYALDEGNYLVLKLLQSYG
                     ALKGERPEQVEQ"
BASE COUNT          608 a          703 c          688 g          648 t
ORIGIN      
        1 ctgatgatgc gaccctgatt tatccggggg caaaaacaat tgttgacttt agcagtggtg
       61 atggaagagc gatgcaactg caaacgggtg actcgctcga gcaggtgatt gcgtggtacg
      121 agaagagcat taagccgacc aagacaatgc ggctgacttc taccagcgtg gtgcttaaga
      181 atgaaaatgt gactacgaca ttagccagcg aaggcggcaa aacgaacatt ttgatcaaac
      241 gagtgaagtg attggcgatt gctctcattg ccgattgcaa attgcgaatt ggttcagtaa
      301 gcgagagatt agcttattgg aagatgagca aatggatcgt aaattggcaa acggcaattg
      361 gcaatgacat gaatcggcaa tcgccaattg gcaatcggca atctcaatga ccttattcgt
      421 cggcacgagc ggctacagct acaaagaatg gaaaggaagt ttctatcccg agaagctccc
      481 ggcaaaggag atgctcgcgt actacgccac caaactccca gccgtagaaa tcaataatac
      541 gttttaccgt ctgccacaaa agtcagtcct tgaaaactgg aaagaacagg tgccagagag
      601 ctttcgcttt tcagtcaaag cctcgcagcg gattactcac ttcaagcgcc tcaacggtgt
      661 tagcgacgaa acgagctact tgcttgagac cgcagcggcg ctgggcgatt gcctcggacc
      721 aattctcttt caactgccac cgaacatgaa gaaggacctc gcgcgtctgg aaacgttcct
      781 cagcactctt cccgcaggca ccagggccgc ctgggagttt cgccatccca cgtggttgga
      841 agatgatgta ttggaacttt tgtcacggca caaccacgcc ctctgcattt ccgacacaga
      901 tcaaatgccc gtaagtcacg tcgacaagac cgcggactgg ggttatctgc gactgcgccg
      961 ggttagttat tccgctgaga accttgccga atggctgcaa cgagtgcgcg atcagaattg
     1021 ggagttcgct tacgtcttct tcaagcatga agacgaagga acaggccccc gactcgcggg
     1081 ggacttcctc aagctggcgg gagctggcta agcagcacaa aaaacaaaag aggcgtgcgg
     1141 ccacgcctct cgtttaggaa agcttgtcgc gtaaaagcta gaagtttccg ctgcactccg
     1201 ggtgtccgca atcgcaatcg atttcaatcg tgctacctga gccttcacac gaagcgctgc
     1261 aatacttgcc atccggctga ggcggacagc tacatgctgg gttcttgcaa gtgtctttat
     1321 cgtcggccat gagttcctcc taaaaaatcg gtgcttgtct ttgggctgta gatctgggag
     1381 gcgttagatt agcacagtct tatgcctggg aagcatggcg catcccagat gactattgct
     1441 ctacttgctc tggcctttcg cccttcaagg cgccgtagga ttggagtagt ttgagcacca
     1501 ggtaattccc ctcgtcaagt gcatagtcga gagctgtcca tccttttttg tcgcgggcat
     1561 tcatatctgc gccggcgcgc accaaagctc tgacgttggc aacctgctca gaacgagcag
     1621 cgatgatgag ggctgtctgc ccttcgtcat tcttatcctc aaccttcgcc cctgcgtgga
     1681 gcagcgccat taacgccggc aaattcttct ctgaagctat ttccgtcaag gccgtgtcac
     1741 cttcttcgtc cctcagattt accttcgcac cagcgttgat taggtcccag actatttcta
     1801 cggtggtttc cgatccgagc agcatcagcg ccgtttgttt ggaagcgtcg cggctgttga
     1861 cgtccgcgcc cgccgccaac aatacctgaa ccatttcgcg gttgttgttt agaactgcat
     1921 gttcaagtgc tgtccttccg gtatttttat cgcgtacgtt gacattgtct cgcgttagca
     1981 gcctttctag atcctgcagg tcatcgtcat ttgctgctcg gatcagcggt tgctccgctt
     2041 gaatcgccac agcgccggaa agaacctcga gttgttcaga ctgctgttcg ttggcaacca
     2101 cttccaactc atactgaatg acggcaattt ccagcttgga ctcgatggtt tgcgtttcgt
     2161 tggccgtgat tgctaaaccg tggatttcat ggtttgcgaa accagtcgcg gtgatggtca
     2221 agcgataatt ccccggttgc agtccttcca acttgtactc gccaagtgag tcactaatcg
     2281 cccctaagag gtaaggtgcc tcatcgtttc ccaatgtgat tccggctccc tgaataaccg
     2341 caccgtgcgg atcaatcacc cttcccttaa gagtcgctga gagagcgatc acctcaacac
     2401 tggcgccgtt ttcatagctg aacgtgtgct cataacccag gcggtcagct cctggctgcg
     2461 ccatggcgtt tgtcagagtt agcgttgcac taaacgcgcc cgcagcgatc cgcgaaactc
     2521 gtctttgaat ctggtgaact tttttgggaa tcgctttcag taggattgag ccatcaggaa
     2581 gactttggta gcgaacgcat aaacgtccgc gggatttggc gaccagacgt cgcactcgtt
     2641 tgggggt
//
LOCUS       DASUEX010000089         2650 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_19600, whole genome shotgun sequence.
ACCESSION   DASUEX010000089 DASUEX010000000
VERSION     DASUEX010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19600"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1033)
                     /gene="glnA"
                     /locus_tag="VFV61_01530"
     CDS             complement(<1..1033)
                     /gene="glnA"
                     /locus_tag="VFV61_01530"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEX4899180.1"
                     /translation="MNPKSVLKYASDEGAKFVSVRFTDLPGSWQHLTFPIGELDEDKF
                     EEGFGFDASSIRGWAAIHESDMLLVPDAGRAWMDPFSEHPTLCLIGNAIDPITKEGYA
                     FDPRSVAVRAESYLKFTGLADTAYFGPEAEFFVFDSAKFHNDANSAGYTIDSDEAHWN
                     SGRDANEFSGSNLGFRIRPKEGYVPVPPLDSLSDLRSEISLTLLKCGINVECHHHEVA
                     TAGQCEIDFRYSTMLGTADNLQIFKYVVRNTAFQQGKAATFMPKPLYGDNGSGMHCHQ
                     SLWKGDKPLFGGDGYAGLSEMARHYIGGLLKHAAAVVAFAAPTTNSYKRLVPGFEAPV
                     NLAYSARNRS"
     gene            1463..>2650
                     /gene="speA"
                     /locus_tag="VFV61_01535"
     CDS             1463..>2650
                     /gene="speA"
                     /locus_tag="VFV61_01535"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF003763.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HEX4899181.1"
                     /translation="MTTAIEQTLETYGIENWGAGYFDVNRKGNLIVRPAEGDPRSADL
                     KEIIDDLANRGIGAPVLLRFPQLVAAQVRKLQRAFSRSIREFEYQGAHLCVYPMKVNQ
                     QRSVVEEYLREGSRYDFGLEAGSKAELYAALALEQTPDSLLVLNGFKDQEFIELAFAG
                     ARCGKRVVIVIEKLNELDHVLNIAEGQENDLPIIGMRVKLYSKGSGRWEKSGGEAAKF
                     GLTTTEMLEVIRRLEQAGRIDMLRLLHFHIGSQLTDIKRIKNAMKEAARVYAKVYQMK
                     VPVDLLDVGGGMAVDYDGSKTAFDSSANYTAQEFANDVIYTIKQVCDDENVPHPTIIQ
                     ESGRYLAAYHAILVTNVQDEIETVVEDITPIEIDDDDPQVVTELGDLRASITVKNYRE
                     YYHD"
BASE COUNT          647 a          691 c          727 g          585 t
ORIGIN      
        1 ccgagcggtt ccgcgccgaa taagcgaggt ttaccggggc ttcaaaccca gggacgagac
       61 gcttgtagct gtttgttgtg ggtgctgcaa aggcgacgac ggccgccgcg tgttttagta
      121 gtccgcctat gtaatggcgc gccatctcag aaagaccggc ataaccgtca ccgccaaata
      181 gtggcttatc acctttccac agcgattggt gacaatgcat gccggacccg ttatccccat
      241 aaagcggttt cggcataaag gtcgcagcct taccctgttg gaacgcagta ttgcgaacta
      301 catatttaaa gatttggaga ttgtcggcag tccccaacat cgtggagtag cgaaaatcaa
      361 tctcacactg accagcggta gcaacctcat gatgatggca ttcgacgttg atcccacact
      421 tcaacaatgt cagtgaaatt tctgaacgca ggtcgctcaa cgagtcgagc ggcggcaccg
      481 gcacataacc ttctttggga cggatgcgga aaccaagatt agaaccggag aattcgtttg
      541 cgtcgcggcc gctgttccaa tgggcctcgt cagaatcgat cgtgtacccg gcggagttgg
      601 cgtcgttatg aaacttggcg gaatcgaata caaaaaactc ggcttcgggt ccgaagtagg
      661 cagtgtcggc cagaccagta aacttcaggt aactttccgc tctgactgca accgaacgtg
      721 gatcgaaggc ataaccttct ttggtaatcg gatcgatggc gttgccaatg aggcaaagcg
      781 tggggtgctc cgaaaaaggg tccatccacg cccgcccggc atcggggacc aagagcatgt
      841 ccgactcgtg aatggcggcc cagccgcgga tcgaagaagc gtcaaaaccg aagccttctt
      901 cgaacttgtc ttcgtccaac tcgccgatgg ggaatgttaa gtgctgccat gagcctggca
      961 gatccgtgaa tcgcacggac acgaatttgg cgccttcatc cgaggcgtac ttcaagacag
     1021 actttgggtt catgagttct cctgactgtt aaagggagtg ttaggggtgc tcttcgcccc
     1081 ggcgtggttg atggtgcgaa gtggctgaaa agctgaagtg atccgtagaa aaaggcgaac
     1141 gtcatggagc gacggcgcgt aaaaaagttt gcattatagt tagccagact actatgtcaa
     1201 gatgtttgcc gcaactgacg acgagcggag tgcccagact ggaattaacg tgcttaatga
     1261 tgacgtttta actgcttttt cccaccgcac cgctccaaaa tagttataga ccgaccccct
     1321 gatttctgtt acattgcgac gactaagcgt tccatcccat agaaaaactt ccgccctcga
     1381 ttcggatgaa atacacccgc gtatttttat cagcaattat tggcatggaa ggagttttgc
     1441 agtttagtca ggagaccgcg aattgacgac ggcaatcgag cagacgttag agacttatgg
     1501 catcgagaat tggggcgctg gttactttga cgtaaatcgc aaggggaatc tgatcgtccg
     1561 acccgccgaa ggggatcctc gctcagccga ccttaaagag attatcgacg acctcgccaa
     1621 ccgtggcatc ggcgcacccg tactccttag gtttccgcaa ttagttgctg cccaggtcag
     1681 gaaacttcaa cgcgcattca gcaggtccat tcgagaattt gagtatcagg gtgcgcacct
     1741 ctgtgtctac cccatgaagg tgaaccagca gcgttctgtt gtagaagagt atctccgtga
     1801 aggttcgcgc tacgacttcg gacttgaagc cggctctaag gccgagcttt acgcggcgct
     1861 cgcgttggag caaacacccg acagcctgct ggttttgaat ggcttcaagg atcaggagtt
     1921 cattgagctt gcttttgctg gagcacgttg cggcaagcgc gtcgtgatcg tgattgagaa
     1981 actcaacgaa cttgatcatg tgctgaacat cgcggaagga caagaaaacg atctgcccat
     2041 catcggcatg cgcgtcaagc tttattcgaa gggctcaggt cgctgggaaa aatccggagg
     2101 cgaggcagca aagtttggcc tcacaaccac tgagatgctt gaggtgatcc gccgcctgga
     2161 acaggcgggc cggatcgaca tgctgcgttt gctgcacttc catatcggct cccagcttac
     2221 tgacatcaaa cgtattaaga atgcgatgaa ggaagccgcg cgcgtttacg ccaaggtcta
     2281 tcagatgaaa gtgccggtcg atctgctcga cgtcggcggc ggcatggccg tggattacga
     2341 cggttctaaa actgcttttg actcttcggc gaactacacg gcccaggagt ttgcgaacga
     2401 cgtcatctac acgatcaagc aagtctgtga tgacgagaac gtgccccacc cgacaatcat
     2461 tcaggagtcg ggccgctacc tcgctgccta tcacgcgatc ctcgtaacca atgttcagga
     2521 cgagatcgaa accgtcgtgg aagacattac gcccatcgaa atcgacgacg acgatcccca
     2581 ggtggttacg gaactcggcg acctgcgcgc gtcgatcacg gtaaagaact atcgcgagta
     2641 ctaccacgac
//
LOCUS       DASUEX010000090         2212 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_477742, whole genome shotgun sequence.
ACCESSION   DASUEX010000090 DASUEX010000000
VERSION     DASUEX010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477742"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(80..1564)
                     /gene="ffh"
                     /locus_tag="VFV61_01540"
     CDS             complement(80..1564)
                     /gene="ffh"
                     /locus_tag="VFV61_01540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEX4899182.1"
                     /translation="MFESLSDKLKRTLKNLRGEGVLTKEHVDAALREIRLALLEADVN
                     YKVAKDFIASVKEKAEGQQVWQDLKPSEQVVKIVFDELVELLGGQSSRLVFTKQIPNA
                     VMIVGLQGSGKTTSTGKIARWLAQNQDRKPLLLSVDVYRPAAREQLKVIARATGQQIF
                     EQPETNDPLTLVREAVKHAQQTGFDTLLIDTAGRLHIDDALMNELVMIKNETRPNEIL
                     FVADAMTGQDAVKSAEEFHRRVGITGVILTKMDGDARGGAALSIKQVTGQPVKFVGVG
                     EKYDALEPFYPDRVAQRILGMGDVLSLIEEVQSKVDQSEAEEQLKKLQKNEFTLDDFR
                     SQLRQVKKLGSFSKIMKLLPDQLLGGIGMPQLDDEQTKLMEKELKRTESIIDSMTREE
                     RGDHRILNANRRRRIAKGSGTSVAEVNQLIKQYSEMRQMMRQLSGSGIFGGGGLKSKM
                     LRRMTGMPDLSGFGSNGEEMGSLPSLPAGPSKKKLKKKRKKRRR"
     gene            1680..>2212
                     /locus_tag="VFV61_01545"
     CDS             1680..>2212
                     /locus_tag="VFV61_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899183.1"
                     /translation="MRLNKRHLYRTFYLFLLTSFLVGALWFPSSHARLNLVVKTSSAR
                     ANLEFQPIAPGIEYLQVVRRSASNEAGNGPWLINALRVDLKMARLQLARALDEGVGLE
                     TVSSLAARHEAVAATNAGYFRTTGTYRGESVGTFLLGGKLFSEPHNDRAALGLTEANG
                     VVFGRLKFFGEISAGKM"
BASE COUNT          461 a          619 c          573 g          559 t
ORIGIN      
        1 cgcgccggtg cgcatcaatt tgatggctaa caaattatta cctccgatgg tgttgctacc
       61 aactggtagc agagttgact taccttcgtc gcttctttcg cttcttcttc agcttctttt
      121 ttgaaggccc tgcgggcagt gaaggaagag agcccatctc ttctccattg ctaccaaacc
      181 cgctgaggtc cggcatgcca gtcatgcgcc gcaacatctt actcttgagc ccgccgcccc
      241 cgaaaatccc ggagcccgaa agttgccgca tcatctggcg catctcgctg tactgtttga
      301 tcagttgatt tacttctgcc accgaagtac ctgatccttt tgcgattctg cgacggcgat
      361 tggcattcaa gatcctgtgg tcgccacgct cctcgcgagt catcgagtcg atgatggatt
      421 ccgttcgctt cagttctttc tccatcaact tcgtctgctc atcgtctaac tgcggcatgc
      481 cgatgccacc gagaagctga tccgggagca gcttcatgat ttttgagaaa gagccgagtt
      541 tctttacttg tcgcaattga gatcgaaaat cgtcgagcgt gaactcgttc ttctgaagct
      601 tctttagttg ttcttcagct tccgattgat cgaccttcga ctggacttcc tcaatgagcg
      661 ataaaacgtc gcccattccc aggattcgtt gggccactcg atctggataa aagggctcga
      721 gcgcgtcgta tttctcgccg acgccgacaa acttgaccgg ctgcccggtg acttgcttga
      781 tcgacagtgc cgcaccgccg cgagcatcgc cgtccatttt cgtcaaaatc acgccggtga
      841 tgccgacacg acggtgaaac tcttccgctg acttgaccgc atcctgtcct gtcatcgcat
      901 ctgcgacaaa cagtatttcg tttggacgcg tttcgttctt gatcatgacg agctcattca
      961 tgagcgcatc gtcaatgtgc aagcggcccg ccgtatcgat cagcaaggtg tcaaatcccg
     1021 tttgctgggc gtgcttgacc gcttcgcgca ccagcgtgag cggatcattg gtttcgggtt
     1081 gttcaaagat ctgctggccc gtcgccctgg caatgacctt taactgctcg cgcgccgcag
     1141 gccggtaaac gtcaacagag agcagcagtg gcttgcgatc ctgattctga gcgagccagc
     1201 gtgcgatctt tccggttgag gtggttttgc cggagccctg aagccccaca atcatcaccg
     1261 cgttcggaat ctgtttggta aagaccaggc gcgaggactg cccgcccagt agttccacca
     1321 gttcgtcgaa aacaatcttg acgacctgct cggacggctt taaatcctgc cagacctgct
     1381 gtccctcagc cttctccttg acagaggcta taaagtcttt tgcgacctta tagttaacgt
     1441 ccgcttcgag tagcgcgaga cggatttcgc gcagcgcagc atcaacgtgt tccttcgtca
     1501 gcacgccttc gccgcgcaga ttcttgaggg tgcgctttaa cttgtcagat agggactcaa
     1561 acatagagca ataggcgaag tcccgcgctg gagacggaaa cggagatgat aacggcgacc
     1621 ttttagggtg tcaaggaagt gtgactgcaa actccaatag ctcagtaatt tgctgaacca
     1681 tgcgactgaa taaacgccat ctctatcgaa ccttttacct gttcttgttg acctcatttc
     1741 tagtcggagc gctctggttc ccatcgagtc atgcgcggct caatctagtc gttaaaacct
     1801 cttcagcccg ggccaatctc gagtttcaac cgatcgcgcc agggattgag tatctgcagg
     1861 ttgttcgacg ctctgcgtcg aacgaagcag gtaacggacc gtggttgatc aacgcactgc
     1921 gagttgacct gaaaatggca aggttgcaac tggcgcgtgc gctagatgaa ggcgtcggat
     1981 tggaaaccgt cagttctctc gctgcgcgac atgaagctgt agctgcaacg aacgccggct
     2041 acttcaggac cacgggtacg tatcgtggcg aatctgttgg cacctttctg ctcggaggca
     2101 agctgttcag cgaaccgcat aatgaccgcg ccgcgttggg tcttacagaa gcgaacggcg
     2161 tagtttttgg tcgtctcaaa ttcttcggcg agatatcagc gggaaagatg ag
//
LOCUS       DASUEX010000091         3287 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_477799, whole genome shotgun sequence.
ACCESSION   DASUEX010000091 DASUEX010000000
VERSION     DASUEX010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3287
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477799"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /locus_tag="VFV61_01550"
     CDS             <1..807
                     /locus_tag="VFV61_01550"
                     /inference="COORDINATES: protein motif:HMM:NF012885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899184.1"
                     /translation="DKLLALTEDAVTFAVSEGVPVMYVTEDTTRADPETLRALYTTAI
                     RCGAKAVCVCDTVGHSTPDGARAVVSFVKSIIQEQGGGVRLDWHGHQDRGLGVINSIA
                     AIEGGADQVHGSALGMGERVGNTPMDQLLVNLKLMGWIDNNLSRLGEYCALASAACDW
                     PIPHNYPVFGRDAFRTGTGVHAAAVIKSYKKGDRQLADLVYSGVPAGQFGLEQVIEIG
                     PMSGKSNVIYWLEKRGIEPSEERVNRIYEHAKQASCVLAEEDIRELVSQS"
     gene            1032..1577
                     /locus_tag="VFV61_01555"
     CDS             1032..1577
                     /locus_tag="VFV61_01555"
                     /inference="COORDINATES: protein motif:HMM:NF044704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HEX4899185.1"
                     /translation="MGFKIGQKVVYPNHGIGTIEQIEKKQIGATALPFYTLRLAANNS
                     LVLVPVSNATEVGLRAPISSGECEMLFKSLADDFTPPANDWKDRFKEFSDKMRTGDIF
                     EVADVLKHLTYLSHLKPLSFREQRMLERSHYLVISELAAVCRQPECNIEPRVEQALAR
                     ACTKHERRPARAQAAGAAAGH"
     gene            1761..3176
                     /locus_tag="VFV61_01560"
     CDS             1761..3176
                     /locus_tag="VFV61_01560"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEX4899186.1"
                     /translation="MSEPPSYLYNLILADGEAGASIGLLMIVLKIGVVLFLVAVNGFF
                     VAAEFALVGVRRSRIETLAAAGKSSAKRLLGLLDDLNAYLSASQLGITLASLGLGWVG
                     EPVVARMLEGPLSGLTDTWRHGISFFIAFAIITALHIVLGEQAPKLLGLERAERVALA
                     VSLPMKSFYRVFQWPIRALDWASGRTVQLFGLKASSEHGSIYTEAELRQLIDVSRQSG
                     HLRAEERRLIHRVFEFSDTLVREAMVPRTQIAALSSECKLEEIAHAFEQYRFSRLPVY
                     RESIDDVIGFVHSKDVMPYLLQPETFKLQDVIQPPLYVVDTARLEDVLRQMQRGKSHF
                     GFVVDEHGGLEGIITLEDLLEEIVGEISDEHDEEVNEQIRQVNECEYQLEGGLAVRDL
                     NRRLKLSVPESESYTTIGGFLMTAAGHVLKPGEVVRHNGLMFKVERVERRRVMRVKLT
                     LTPETSEAEGQPTAEGARAAG"
BASE COUNT          703 a          913 c          924 g          747 t
ORIGIN      
        1 gacaagcttt tggcgctgac tgaggacgcc gtaacgtttg cagtgagcga aggcgtgcct
       61 gtgatgtatg tgacagaaga caccacgcgc gctgatcccg agacgttacg tgcgctatac
      121 acgacagcaa tccgctgcgg tgcgaaggct gtttgcgttt gcgatacggt gggacactcc
      181 acgccggacg gcgcccgcgc tgtagtaagc ttcgtgaaga gcattattca agagcaaggc
      241 ggcggggtgc ggctcgactg gcacggtcac caggatcgtg gtctcggcgt tattaattct
      301 attgccgcca tcgagggagg cgccgatcaa gtccacggtt cggctttggg catgggcgaa
      361 cgcgtaggca acacgccgat ggatcagctg ctcgtgaacc taaagttgat gggttggatt
      421 gataacaacc tgtctcgtct cggcgaatat tgcgcattgg cgtcagcggc ttgcgattgg
      481 ccaatccctc acaactatcc ggtcttcggt cgcgatgcat tccggaccgg aacgggcgtg
      541 cacgccgccg cggtgatcaa gagttataag aagggcgatc gacagcttgc ggacttggtt
      601 tattcaggtg ttcccgccgg ccagttcggg ctggaacaag ttattgaaat cggaccaatg
      661 agcgggaagt cgaatgtgat ctactggctg gagaagcgcg gcattgagcc cagcgaggaa
      721 cgcgtgaatc gcatttatga acatgcgaag caggcttcgt gtgtccttgc ggaagaagat
      781 attcgggaac tcgtttcgca atcttaacca gcctcgcaac agattcactc aacgaaacga
      841 tctattcctg gcttggccag aatttctcgt cgctaggcgg cagctgtaaa ccttgacgct
      901 gtgtgagacg ccttgctaga cttcgttcac ttaccaacta ggagcccgac gccaactcgc
      961 gtcgttcaga gggttccccc agacccgcgc gtgcggcgct gtctgatttt caaaacgatg
     1021 gaggtgcttc ggtgggcttc aaaatcggcc agaaagtcgt ttatcccaat cacggaatcg
     1081 gaacaatcga gcagatagag aagaagcaga tcggagctac agcgcttcct ttctacacgt
     1141 tacgcctcgc cgctaacaat tccctcgtgc tcgttcccgt ttccaatgcc actgaagtcg
     1201 gcctgcgagc tccgatctct tcaggtgaat gcgaaatgtt gtttaaaagc ctcgccgacg
     1261 acttcactcc ccctgccaat gattggaagg atcgcttcaa ggaattctca gataagatgc
     1321 ggaccggcga tatcttcgaa gttgccgacg tgctcaagca cctgacttac ctgagccacc
     1381 tcaagccgct gtcgttccgc gaacagcgca tgctggaacg ttcccactac ctcgtcattt
     1441 ccgagttggc cgccgtttgc cggcaaccgg aatgcaatat cgaaccgcga gtcgagcagg
     1501 ctctcgcgcg cgcctgcacc aagcacgagc gacgtcctgc gcgggcccag gccgctggcg
     1561 ccgccgccgg ccactaacgc tccagtctct tcaatctggc ttttgcaaaa acacgtccca
     1621 ggcccagcgt ccggcttgtg cggtctattg gtcagcttgg ctcgattgcc ccgcgcttcg
     1681 tggtcagccc ctcgcccctc atgtataatc cggggccagt tcccatgcag gtccattgga
     1741 acccaccccc tttttgaaga atgtcggaac cgccaagtta tctctacaat ttaatactcg
     1801 ccgacggtga ggcaggcgcc tccatcggat tgttaatgat cgttctgaaa atcggggtag
     1861 tgctgttcct ggtcgcagtt aacggctttt tcgtggcggc cgaatttgcg ctcgttggtg
     1921 tgcgccgctc ccgtattgaa accctcgccg ccgccggcaa gagttctgct aaacgcttgc
     1981 tgggtctgct cgacgatctc aacgcctatc tctccgcttc acaattaggg atcacgctcg
     2041 cctctctggg cctcggttgg gttggcgagc cggtagtcgc acgcatgttg gaaggaccgt
     2101 tgtccggact tacggacaca tggcgacatg gcatttcgtt ttttatcgcc tttgcaatca
     2161 tcactgcgtt gcacatcgtg cttggtgaac aagccccaaa acttctcggc ctggaaagag
     2221 cggagcgtgt tgcgcttgca gtttccctgc caatgaaatc gttctatcgg gttttccagt
     2281 ggccgattcg ggccctggat tgggctagtg ggcgcaccgt gcaactcttt ggcttaaagg
     2341 cgtcttcaga acacggctcg atttataccg aagctgagtt gcgacagttg atcgacgttt
     2401 cgcgccaaag cggtcatctg cgcgccgaag agcgacgctt gattcatcgc gtgttcgagt
     2461 tttcagacac actcgttcgc gaagcgatgg tgccgcggac gcaaatcgcc gcgctctcaa
     2521 gcgaatgcaa gctggaagag attgcccacg cctttgagca ataccgcttt tcacgacttc
     2581 cggtttatcg cgaatcgatc gacgacgtga ttgggtttgt gcatagcaaa gacgtcatgc
     2641 cgtatttgct gcaaccggaa accttcaagt tgcaggacgt gatccagcca ccgctttatg
     2701 tggtggacac ggcgcgtctt gaagatgtac tgcgacaaat gcaacggggc aagtcacact
     2761 tcggcttcgt cgttgatgaa catggcggcc tggaaggaat catcactctc gaggatctgc
     2821 tggaagagat cgtcggcgag atttctgacg agcacgacga agaagtcaac gagcagatca
     2881 ggcaagttaa tgagtgcgaa tatcaactgg aaggcggact cgccgttcgc gatttgaatc
     2941 gccggctgaa gctttcggtt ccggaatcgg agagctacac cacaatcggt ggattcctga
     3001 tgaccgccgc cggccatgtg ctgaaacccg gcgaggttgt gcggcacaac ggcctcatgt
     3061 ttaaggttga gcgcgtggag cgccgtcgag taatgcgcgt gaaattgact ctcacaccag
     3121 aaacctcgga agcggaaggc caacctaccg cagaaggggc acgcgccgcc ggttagaaca
     3181 agatcctgtc cacgtctcac cttccttcaa tcacactgca acgtgataaa gttgtagctc
     3241 gaaaaacgcc ttaacattct tttccgtgga gttgacttaa agatgag
//
LOCUS       DASUEX010000092         5189 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_238245, whole genome shotgun sequence.
ACCESSION   DASUEX010000092 DASUEX010000000
VERSION     DASUEX010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5189
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238245"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..799)
                     /locus_tag="VFV61_01565"
     CDS             complement(<1..799)
                     /locus_tag="VFV61_01565"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX4899187.1"
                     /translation="MTPFATHLVHRLALLLCCFTAAAAMAAAQQQTPKASPTPPLTAD
                     DQTPITTFIRRVRLPITVVDKKGQFVPGLTKTDFLVFEDKQPQQIETFSDDLGESLPL
                     YVAVLMDTSPSTAGKLKFEQESAMNFIQTVVRPRRDRVLFATFDHQIELKQDFTDKLD
                     LLDRAVFGVKKLGTQTALYDAIWQFCDQKLRSVSGRRVLVVITDGEDTYSRADLSDAI
                     DIAQRTETTIYVISTKAGLSGTVPGVRLGEVKDKFDNIIVKLAEETGG"
     gene            1080..3629
                     /locus_tag="VFV61_01570"
     CDS             1080..3629
                     /locus_tag="VFV61_01570"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX4899188.1"
                     /translation="MPDLNSPKNEAGPPSPEFVAHYRIIKRLGKGGMGEVFLAEDTRQ
                     HNRKVALKILPPQLTSEEGRVRRFKHEARAILALNHPNILTIFEIGETADTHYIATEF
                     IDGETLRQCLWRQSIKIDEALGIAIQVAMALEAAHAAGIVHRDIKPENIMLRHDRFVR
                     DRFVKVLDFGLAKLTENESRLADPEAATITNTNPGTILGTTGYMSPEQAQGSAIDMRS
                     DIFSLGVVLYEMIAGRAPFTGPTDSHVRVSIIDQEPAPLSQSGEVPRQLDRIVSKALA
                     KDRSKRYQTITDLKLDLEQLRSELADPKERSETIHDHLAKTIAGRPTFDRVTSTDTTR
                     IIAKTTVDAKARRGFFRVYKTPLVIAFVALIAVVGVFAYFKYSRPKINSVAILPFVND
                     SGDPNAEYLSDGITESIINSLSRLPGLKVMSRNAVFRFKGKNPDPQEVGRNLRVGAVL
                     MGRLVKLNNRLIIKTELIDVADGSQLWGAEYNNSPSDILAVQDEIARAISAKLQLRLT
                     GDDEEKLAKRYTADAEAYELYLKGRYFWNKRGEAGLRNGIKYFQEAEQKDPNYALAYS
                     GLADSYALLCDIGVVAPVTEMPQAKAAAQKAVDIDPTLAEGYTSRGFIKLAYDWDWLG
                     AEADLKRGLELNPKYPTAHQWYASYLVQMGKFDRAKQEIEQAQQLDPLSPIISSNAGL
                     YSYFEHRYDDAIAQYQKTLEIDPTFWVAHHYLGLAYAKKGMSDQAISELRNSLYSPGT
                     GPLKEGAVENDPEIAASLGYAFGLAKRRTEAEAILNRLRALAQRRYVSGLYFAIVYTG
                     LGDRDRAIQYLNKAFADRHPGLVLIRVDPIFDELRSDKRFQELIARFEPLP"
     gene            complement(3863..4837)
                     /locus_tag="VFV61_01575"
     CDS             complement(3863..4837)
                     /locus_tag="VFV61_01575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEX4899189.1"
                     /translation="METKDAKSPHADPTPILTFKIDQTHADTRLDAYLASQIEGWSRA
                     RLQNLIADEEVLVNGKPAKSSYKLNANDEIEVELIAAPTTTFAPENIPLDIVFEDDAL
                     IVVNKPAGLVVHPAAGIQSGTLANALAFHFDQLSKKAGTMRPGIVHRIDKGTSGLLVV
                     AKTEMAHEHLADQFRERKVFKSYLALVHGVVERDAGRIEQPLARDPRNRTRMAIVRGG
                     RGALSLYKVRRRFDRFTLLDVEIKTGRTHQIRVHLASLKHPVVGDEVYGAGRDNTVRD
                     PKLRAEIRKLGRHFLHAAELGFRHPVTGEQLRFQSPLPTELQHLIDLL"
BASE COUNT         1249 a         1404 c         1372 g         1164 t
ORIGIN      
        1 tgccaccggt ttcttctgcg agtttgacga taatgttgtc gaacttatct ttcacttcgc
       61 cgagcctcac tcccgggaca gtccccgaca atccagcttt ggtagaaatt acgtaaatag
      121 ttgtctcggt ccgctgcgcg atatcgatcg cgtcgctcaa atccgcgcgg ctgtaagtat
      181 cctcgccatc agtaatgaca accaaaactc ggcggccgga aaccgagcgc agcttctggt
      241 cgcagaattg ccagatggcg tcgtacaaag ctgtctgcgt tccgagtttt tttacaccaa
      301 agactgcccg gtccagcaga tccagtttat ccgtaaagtc ctgcttcagt tcgatctgat
      361 ggtcgaaggt ggcgaagagc actcgatccc gtcgcggccg aaccacggtc tgaataaaat
      421 tcatcgccga ctcttgttcg aattttagtt tgcccgcggt tgaaggtgac gtatccatca
      481 acaccgctac gtagagcggc agactctcgc ccaaatcgtc ggagaacgtt tcaatctgct
      541 gtggctgctt atcttcaaaa acgaggaagt cagtctttgt caggccggga acgaattgac
      601 ctttcttgtc gaccacagta ataggcaaac gcacgcggcg aataaaagta gtaattggag
      661 tttgatcgtc tgccgtaagc ggcggagtag gactcgcttt cggagtttgt tgttgggcgg
      721 cagccatggc cgcggcggcc gtaaagcaac acagtaagag tgccagtcga tgaacaaggt
      781 gcgtcgcgaa aggggtcatg atctaaaagc cagttgcttg tcgatttttc ttaagattag
      841 ctgctcaaag ccggcgattc gatgccacgg caactggatc tcgttgcccc agagccttcc
      901 acacggcggc agcgaacaaa tcccaaatat gaaaacatat cgggcgcaac taaccaagcc
      961 tgctcgtatt aatcactcat ttcgtcgctt aggcactttg tagttcgcaa tactacgcgg
     1021 tgatacaatg cgcgcattcc gaacgactgc gcatcgttgc attgagggac acgtgcttca
     1081 tgcctgatct caactccccg aaaaacgaag ccggcccgcc ttccccggaa tttgtcgctc
     1141 actatcgaat catcaaacga ttgggcaaag ggggaatggg tgaagtgttt ctcgcggagg
     1201 acacgagaca acataaccgc aaagtggccc tcaagatatt gccaccgcaa ctgacgagtg
     1261 aggaaggtcg cgtacggcgc ttcaaacacg aggctcgtgc catcctcgca cttaaccatc
     1321 ccaacattct taccatcttt gaaatcggcg agaccgcaga cactcactac atcgccacgg
     1381 agtttatcga cggtgaaaca ctgcgccagt gcctgtggcg acagtcgatc aaaatcgatg
     1441 aggcgttggg gattgccatt caagtggcaa tggccctgga agcagcgcac gccgcgggaa
     1501 ttgtgcatcg cgacatcaaa ccggaaaaca tcatgctgcg tcatgatcgc tttgtgcgcg
     1561 atcggttcgt gaaggtcctg gactttggcc tggccaagct gacagagaac gagtcacggc
     1621 tggctgatcc agaagcggcg acgataacca ataccaaccc gggcaccatt ctcggcacca
     1681 ccggctacat gtctcctgaa caagcccagg gcagcgccat tgacatgcgc agcgacatct
     1741 tcagtcttgg tgtggtgctt tatgaaatga ttgctggccg cgctccgttc actggtccga
     1801 ctgacagtca cgtccgcgtc tcgatcatcg atcaagaacc cgcccccctc tcgcagtcgg
     1861 gagaggtgcc gcggcaattg gaccgcatcg tctcaaaggc gctggccaag gatcgcagca
     1921 agcgttacca gacaatcact gatctaaaac tcgatctcga gcagttgcgc tcggaactag
     1981 ctgatcccaa agagcgatcc gagacaattc acgatcactt agcgaagaca attgccggac
     2041 gtccgacctt tgaccgggtt acctcgacag ataccactcg catcatcgcc aagacaaccg
     2101 ttgacgctaa ggcaagacgc ggattctttc gggtctacaa aacacctctc gtgatcgcct
     2161 ttgtcgcgct catcgccgtc gttggcgttt ttgcgtattt caagtactcg cggccaaaga
     2221 tcaactcagt agccattctg ccctttgtta acgacagcgg cgatcccaat gccgagtacc
     2281 tctcggatgg aatcacggaa agcattatca acagcctctc ccgcctgcca ggcctcaagg
     2341 tgatgtcgcg caacgccgtc ttcaggttca aaggtaagaa tcccgatccg caggaagtgg
     2401 gaagaaacct gagggtcggt gctgtgttga tgggccgact cgtcaaactt aacaaccgtt
     2461 taataatcaa gacggagcta atcgatgttg ctgatggctc acagctttgg ggcgctgaat
     2521 acaacaatag cccctccgac attctcgccg tgcaggatga gatcgcgcgc gcgatctctg
     2581 caaagttgca gcttcgtctg actggggatg acgaagaaaa gctggccaag cgttacaccg
     2641 ctgatgccga agcatatgag ctttatttaa agggtcgcta cttctggaac aagcgtggtg
     2701 aggctggcct ccgcaacgga atcaaatatt ttcaagaggc ggaacagaag gatccaaact
     2761 acgcactcgc atactctggc ctcgccgatt cctacgccct gctatgtgac atcggcgtag
     2821 ttgcgcccgt caccgagatg ccacaagcga aagcagcagc gcaaaaggca gtagacatcg
     2881 atccaacact cgcggaaggc tacacctcac gagggtttat caaacttgca tacgattggg
     2941 attggttagg agcggaagcc gatttgaagc gcgggttgga actcaatcca aaatatccca
     3001 ccgcccatca atggtatgcc tcgtatctcg tgcagatggg aaaattcgat cgcgccaaac
     3061 aagagatcga acaagcacag caactcgatc cactctcgcc tatcatcagt tctaatgctg
     3121 ggctctattc ctatttcgaa catcgttacg acgacgcgat cgcgcagtat caaaagaccc
     3181 tcgagattga tccaacattt tgggtagcac accattatct gggactggcg tacgccaaaa
     3241 aaggaatgag tgaccaggcc atcagtgaac tgcgtaactc tctctattcg cccggaacgg
     3301 gtccgctgaa agaaggtgcg gtagaaaacg atccggagat tgctgcctcg ctgggatacg
     3361 cttttggcct ggcaaaacgt cggaccgagg ctgaggcaat ccttaatcgc ctgcgagcgc
     3421 tggcgcagcg gcgttatgtg tcaggacttt attttgcgat tgtctatacc ggactcggtg
     3481 acagggatcg ggcgatccag tacttgaaca aagcctttgc agaccggcat ccggggctgg
     3541 tgctgatacg cgtggatcct atatttgacg aactccgctc ggacaagcga ttccaggaat
     3601 tgatcgcgcg cttcgagcca ctgccttaag ctgattggag ccttgccacg acagcggcct
     3661 taaaaacctc cgtggttacg gcttcggctt tgccgtcggg tgtgaggcgg tggctctgcc
     3721 ccgccgtcgc agcccagatt actgccgggc tctgcccgtg gtgcgggctt ggcccgtcag
     3781 atttgttggg ccaaagggcc gaaccggagg catagccgaa atgtaaaagg cactccacgc
     3841 gttccaaggc gcggtcgcac gctcaaagta gatcaatcaa gtgttgcagt tcagtgggta
     3901 atggagactg gaagcgaagc tgttcgcctg tgactggatg acgaaatccg agctcggctg
     3961 cgtgcaggaa gtgccggcct agcttacgaa tctccgctct caacttcgga tcgcgaactg
     4021 tgttgtctct gccggcccca tatacctcat caccgacaac cggatgcttg agagaggcga
     4081 gatgaactct aatttgatga gtccggcctg tcttgatttc aacatcaagt agggtgaatc
     4141 gatcaaaacg acgccgcact ttgtagagcg ataacgcgcc tctcccgcca cgtacgatcg
     4201 ccattcgcgt acgattccgc ggatcacggg ctagcggttg ttcgattcgg ccggcatcgc
     4261 gctcgacgac cccgtgaaca agagcaagat aagacttgaa aaccttccgc tcgcgaaatt
     4321 gatcggccaa atgctcgtgg gccatttcag tcttggccac caccagcaaa ccggaagtac
     4381 ctttgtcgat ccgatgaacg atgccaggtc gcatcgtgcc cgctttttta gaaagctgat
     4441 caaaatgaaa cgcgagtgcg ttggcgagtg tgcccgattg aattccggcg gcgggatgca
     4501 ctaccagacc ggcgggtttg tttacgacaa tcaacgcgtc gtcttcaaaa acgatatcga
     4561 gaggaatgtt ctccggagca aacgtcgtgg ttggtgcggc gattagctca acctcaatct
     4621 cgtcgtttgc atttagcttg taggacgact tggcgggttt tccgttaact aatacttctt
     4681 cgtcggcaat gagattttgt agacgcgccc tgctccaacc ttcgatctgt gaggcgagat
     4741 aggcatcgag gcgggtgtct gcatgggtct gatcgatttt gaaagtcagt atgggcgtgg
     4801 gatcagcatg cggagacttg gcatctttag tttccatttt catctcaatg gcagaggtgt
     4861 aaaccccaag acgccgatgc tttgattaac cgccaaggcg ccaagacgca gagaacgcca
     4921 agaagcattc aacagctaag gtgaaactgt taagttctga ttgaggttca agtcagctgt
     4981 tcagtcttct ttgcgttctc tgcgtcttgg cgacttggcg gtttcacctt ctaaaaaagg
     5041 aaaacgagcg ttgaaacctc acccgttggt gcgttcaaat tctttcatga acgaaacgag
     5101 agcagtgacg ccttctttag gaaatgcgtt gtagatggaa gcgcgaatac cgcccaccga
     5161 acgatgcccc ttcaagccat ctaaacctt
//
LOCUS       DASUEX010000093         3590 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_822011, whole genome shotgun sequence.
ACCESSION   DASUEX010000093 DASUEX010000000
VERSION     DASUEX010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3590
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_822011"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="VFV61_01580"
     CDS             complement(<1..442)
                     /locus_tag="VFV61_01580"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HemK/PrmC family methyltransferase"
                     /protein_id="HEX4899190.1"
                     /translation="MAASIAVLILEASKLLHSAGVPDSRREASALMGHVLGRDRTFII
                     SHSETQISDDQLAEYRRVVHRRSAGEPLQYITGTQEFFKLDFYVTTDVLIPRPETELL
                     VETALDLIPKKDSSALICDVGTGSGCIAISLLSERPNAKAVAIDI"
     gene            712..1080
                     /locus_tag="VFV61_01585"
     CDS             712..1080
                     /locus_tag="VFV61_01585"
                     /inference="COORDINATES: protein motif:HMM:NF039183.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5658 family protein"
                     /protein_id="HEX4899191.1"
                     /translation="MGTLYKSLLLFGMNWMDAQLTLLWIRLNVATEGNALMARLLDHG
                     ALPFLSVKLAVGAVAAYVLYRSAEIPMAKLGMKVVLGIYGLLMMVHLITGLSVLGWHV
                     PQNALTYLSSLPSALLTFFS"
     gene            1331..2497
                     /locus_tag="VFV61_01590"
     CDS             1331..2497
                     /locus_tag="VFV61_01590"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX4899192.1"
                     /translation="MYKLSARQILVIALVSGLFAAIAVAVVDRVSNRFQPSSAAFSEN
                     APAGITDPAMASDEQNNIEIYRTLSPGVVNIHSTSYARDFFGFVEPQEGSGSGSILDQ
                     EGNILTNYHVIEGAQKVSVSLGGQRVYSARVIGGDPDTDLAVIKLVEKPRDALTVVPL
                     GDSDRLVVGQKVLAIGNPFGLDRTLTTGVISGLQRPIRARNGRPIEGAIQTDASINPG
                     NSGGPLLDSHGRMIGINSQILSPTGASAGVGFAVPVNIAKRIVPQLVRNGVVNRPKLG
                     ISTRDVEALKGQVDLPVPDGVLIWQLAPGGAAASAGLRGLTQTENGDVELGDIIVAID
                     GEKVANTDDLYRVLDKRQVGQTVRVEIVRNGKQMTVPVRLQEAPDSGRGTIRRN"
     gene            complement(2825..3514)
                     /locus_tag="VFV61_01595"
     CDS             complement(2825..3514)
                     /locus_tag="VFV61_01595"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001886.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic pyrophosphatase"
                     /protein_id="HEX4899193.1"
                     /translation="MTNGAGEKQQLFELLFQAHPWHGVSPGTDAPQVVSTYIEIVPTD
                     VVKYELDKGSGHLKVDRPQRYSSLCPTLYGFIPQTFCGSLTAELCAARTGRTGIEGDG
                     DPMDICVLTEKSFGHGGLFLRARPIGGLRMIDGQQADDKILAVLDSDVAYGHITDITE
                     CPQGLIDRLKHYFLSYKQLPGDSPRRVEIVDVYDRSEALDVIQRSMKDYSDRYGDPSK
                     RLSAFRELISP"
BASE COUNT          804 a          957 c          988 g          841 t
ORIGIN      
        1 agatatcgat tgccactgct ttcgcgttcg gacgttcgga caagagactg atcgcaatac
       61 atcctgaccc ggtgccaacg tcgcaaatca gggccgagct gtcctttttc ggaatcaaat
      121 cgagcgcggt ctctaccagc aactcggtct cgggtctggg gattaagacg tcagtcgtca
      181 cgtaaaaatc gagcttaaaa aactcctgtg tgccggtgat gtattggaga ggttcgcctg
      241 ccgagcgccg atggaccact cgtcgatact cggctagctg atcgtcgctg atttgggttt
      301 ctgagtggct aatgatgaac gtgcggtcgc gcccaagcac gtggcccatt agcgcgcttg
      361 cttcacggcg cgagtcggga acacccgccg agtggaggag ttttgaggcc tcaaggatga
      421 gtactgctat tgatgctgcc attcaaactt tccagatccg tgctgcgcca atataatacg
      481 ctgcgaacag ttcaggaatt ttgttgcagc ggcttctgcc acgggacaat ccggacagga
      541 tgcacactcg acttggtttc gggaagatgg accacattgg aattatcttt ctgatacaac
      601 ggacgagtct cgttcctccc gtgtacgacg gagcctccgg agcaaacagc ttcgggggct
      661 ctggcgttaa aggcagacgt ttaacaagtt tggccgcctg aaggagcaat cgtgggaact
      721 ctttacaaaa gcttgttgtt gttcggcatg aactggatgg acgcgcagct cacgctcttg
      781 tggatccgtc tcaacgtggc gactgaggga aatgcgctta tggctcggct gttggaccac
      841 ggggcactcc cctttctttc agtcaaacta gcggttggag ccgttgcggc ttacgtgctt
      901 tatcggtcgg cggagattcc aatggccaag ctcggaatga aggtggtgct gggaatatac
      961 ggcctgctga tgatggtaca cctcataacc ggcctatcgg tgcttggatg gcacgtcccc
     1021 cagaacgctc tcacctatct ttccagtctg cccagtgcgc tgctcacctt tttttcgtaa
     1081 gcgccaatag ttaagtgtcg aatcccatca cgccttagat cctgccgcaa acagtctttg
     1141 aaaccattcc gtttgccacg cgttaatttg tcatgcaaca tgcctggttg cgctcctttc
     1201 aaagctggcc taagttaggc ttcgcgggcg tccctggtga gccgcagttc gaaacgcgca
     1261 accaacctgg gatggccctg catctgaaaa aagtcgcatc aagaatcatt acaaccacag
     1321 gaagtaagtt atgtataaat tatctgctcg ccaaatcctg gttatcgcct tggtttccgg
     1381 attgttcgcg gcaatcgcag ttgctgtcgt cgaccgggta tcaaatcgtt ttcagccatc
     1441 cagcgctgct ttctcagaga atgcgcccgc cggaattaca gatccggcga tggccagcga
     1501 tgaacagaac aacatcgaga tttatcgcac tctttcgccc ggtgtcgtga acattcattc
     1561 gacctcctat gcacgcgatt tcttcgggtt cgtggaaccc caggagggat ccggctcagg
     1621 ctcgatcctc gaccaggaag gaaatatcct gaccaactac cacgttatcg aaggcgcgca
     1681 aaaagtgtcg gttagtctgg gcggtcagag ggtctactcg gcacgcgtta tcggcggcga
     1741 tccggataca gatctcgcgg tcattaagtt ggttgaaaag ccgcgtgatg ccttgaccgt
     1801 cgtgcctttg ggggattccg accggctggt agttgggcag aaggttctgg cgataggaaa
     1861 tccgtttgga ctcgaccgca cacttaccac gggcgtgatc agcggtttgc aacgtcccat
     1921 tcgcgcacgc aacggccgtc cgattgaagg tgcgattcaa accgatgcct caatcaatcc
     1981 cggaaattcc gggggtccat tgttagattc tcacggtcgt atgattggca tcaattcaca
     2041 gatcctgtct cctacgggcg catccgctgg tgtgggtttt gccgttccgg tgaatatcgc
     2101 aaagaggatc gttccgcagt tggtccgcaa cggagttgta aatcgaccga agctgggaat
     2161 cagcactcgc gacgttgaag ctctcaaagg acaagttgat ctgcccgtgc cggacggcgt
     2221 tctcatctgg caactcgctc cgggcggagc tgcggccagc gccggccttc gtggtttgac
     2281 tcagaccgaa aatggcgatg tcgaattggg cgacatcatc gtggccatcg atggtgagaa
     2341 ggttgccaac accgacgatc tctatcgcgt gctggacaag aggcaagtgg gtcaaacggt
     2401 cagagttgag atcgttcgca atggcaagca aatgacggtg ccagtgcggc tacaggaagc
     2461 tcccgacagc ggacgcggaa caattcgacg caattagatg aactaacggt tgttgctcag
     2521 ccgccggctc ccaatggggc cagcggctga gatcaaatct cggacatcgc ctgcggtgaa
     2581 ccagaccaac cacttctaca accttggcta cggctggctt tgcaagcact gtagtgcgga
     2641 agatgcagaa ctaaaacagc acgtcgcaag ccagcatcac gctacgacgg aaactcaagc
     2701 aaaacatgaa ttgccattag ctcgttggac ggatggaact cggcaatccc ttgcctgtcc
     2761 gcgctgtggg atcgaagaga tggtccgcaa gtcttgagtt ctgggactcg cgctagctgt
     2821 ggccttacgg ggagatcaat tcgcgaaacg ctgaaagcct cttactcgga tcgccgtagc
     2881 gatcactgta gtccttcatg ctgcgctgaa tcacatctaa ggcttcacta cgatcgtaga
     2941 catcaacaat ctcaacccgc cgtggggaat ctccgggaag ttgtttgtag gacaggaagt
     3001 agtgcttaag tcggtcgatc aatccctgtg gacactccgt aatatcggtg atgtgcccat
     3061 aagcaacatc ggaatccaaa accgcaagga ttttatcgtc ggcttgctga ccgtcgatca
     3121 tccttagccc gccgatgggc cgagccctga gaaacaagcc cccatgtcca aaagattttt
     3181 cggtcagcac acagatatcc attggatcgc cgtcgccctc gatgccggtg cggccggtgc
     3241 gtgcggcaca gagctctgcg gttaggctgc cgcaaaacgt ctggggtata aagccataca
     3301 aggtcggaca gagggatgaa tatctttggg gacggtccac ttttaggtga cctgaaccct
     3361 tgtccaactc atacttcacc acatcggtgg gaacgatctc gatgtaggtg ctgaccacct
     3421 gcggtgcatc tgttccagga gagactccgt gccatggatg cgcctgaaac agcagttcga
     3481 acagttgctg cttctctcca gctccattag tcataaattg ttcctcggct tccggtgatt
     3541 agttaagtaa gtggtaaaat cctagtctcg cattggtttg gtttcaaccg
//
LOCUS       DASUEX010000094         3895 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_134849, whole genome shotgun sequence.
ACCESSION   DASUEX010000094 DASUEX010000000
VERSION     DASUEX010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3895
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134849"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1352)
                     /locus_tag="VFV61_01600"
     CDS             complement(<1..1352)
                     /locus_tag="VFV61_01600"
                     /inference="COORDINATES: protein motif:HMM:NF012931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEX4899194.1"
                     /translation="MKTLLLIIACTIVVSAQEASTPKLNLMPVPASVIFHRERLAVDS
                     NFRVATRGHSDARLQAAIWRFIKRLEGRTVFTLSPALALDDQTTPLIIHTQGPGKNIP
                     GLGEDESYRIDITNRQAILSAPTVIGAIRGLETVLQLLDADRNGYFLPGVQIDDRPRF
                     PWRGLMIDIARHFQTMDVLKRNLDAMAAVKLNVLHWHFTEDQGFRVESKKFPKLHQLG
                     SDGNFYTQEQVKEVIAYARDRGIRVVPEFDIPGHSTSWLVGHPELGSAPGPYTIERRP
                     GIFEPALDPTREDVYKFLDTFLGEMAALFPDDYLHIGGDENEGKQWDRNPAIQAYMKQ
                     KGIKDNHALQAYFNTRLLKILQKHKKKMVGWDEILQPELPKDVVIHSWRGTAALAEAA
                     RKGYDGILSNGYYIDLIQPASQHYVSDPLPADSTLTPEEAKHVLGGEATMWAEWVTPE
                     T"
     gene            complement(1459..3810)
                     /locus_tag="VFV61_01605"
     CDS             complement(1459..3810)
                     /locus_tag="VFV61_01605"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEX4899195.1"
                     /translation="MRKHLLALWAVSLSLCFAVQPLITIKAQTSNLVDSDEFFQRASR
                     NGPERDHSRKIEDLLKRMTLEEKVGQMTQLSIDQVTSGKDQEIKVDPAKLERAIVKYG
                     VGSVLNVADQALTVDHWHQIIGQIQEAATKKTRLGIPMIYGIDSIHGANYVLGATLFP
                     QEIGMAATFNPELMKRASEITAIETRAAGIPWSFSPVLDLGRNPLWPRFWETFGEDPY
                     LAKVMGVAFVRGLEGTDVSSSNAVATSLKHYMGYSFPLTGRDRTPAWIPENYLREYFL
                     PTFETAIKAGARTIMVNSGEINGVPGHVNRHILTDILRTELGFKGFVVSDWQDIKKLV
                     NVWRIAADEKEATRLAVMAGIDMSMVPNDLSFADHLIALVKEGAVPQSRIDEAVRRIL
                     WVKFELGLFDKPMPDASLKSKFGLPEYRQVSLEAARESITLLKNSNNILPLAKTTKVL
                     VTGPTADSLVSMNNGWTYVWQGSEENLYPKDRHTIRKALEAKLGAGNVTFVPGTKITR
                     APGPSNSTPTDQEEEVDIAAAVQAARNVDVIVLALGEGSYCETPGNVTDLTIGAPQIK
                     LAEALKATGKPIVLVLVEGRPRIINRIADDMNAIVMAYNPSHEGGTAIADVLFGDVNP
                     SGRLPFTYPRTPNGLINYDHKPFETENTSFGNMAFNPQFNFGDGLSYTTFSYSDLRLG
                     KQTISANEELPVSVTVKNTGNRAGKEAVLVYVSDLVASLSPPGKRLRRFAKVSLAPGE
                     SRTVTFKLRREDLSFIGTDNKPIVEPGDFEIRIAGLMQRFTVK"
BASE COUNT          791 a         1056 c         1113 g          935 t
ORIGIN      
        1 gtctccggcg tcacccactc ggcccacatc gtcgcttcgc cgccgagaac gtgcttcgcc
       61 tcttcaggcg tcagcgtgct gtcggcggga agtggatcgg atacgtagtg ttgcgacgcg
      121 ggttgaatca agtcgatgta gtaaccgttc gagagtatgc cgtcgtaacc tttgcgcgcg
      181 gcttcggcga gcgccgcggt gccacgccac gagtgaatta cgacatcctt tggcagctcg
      241 ggttgcagga tctcgtccca gccgaccatc ttctttttgt gcttctgaag aatcttcagc
      301 agccgcgtat tgaagtaagc ctgcaacgcg tgattgtctt tgatgccttt ctgcttcatg
      361 taagcctgga ttgcaggatt gcggtcccat tgcttgcctt cgttctcgtc gccgccgatg
      421 tgcaggtagt cgtcaggaaa gagtgcggcc atttcgccga gaaatgtatc gagaaacttg
      481 tagacgtctt cgcgtgtcgg atcgagcgcg ggctcgaaga ttcccggacg gcgctcgatc
      541 gtgtatggac caggcgcgct gccgagctca ggatgaccga cgagccagct cgtgctgtga
      601 ccgggtatgt caaactccgg gacgacgcgt atgccgcggt cgcgcgcgta cgcgatgacc
      661 tctttcacct gttcctgcgt gtagaagttg ccgtcagatc cgagttggtg cagctttgga
      721 aactttttac tctcgacgcg gaagccctgg tcttcggtga agtgccagtg caggacgttc
      781 aacttcacgg ccgccatagc gtcgagattg cgcttgagaa cgtccatcgt ttggaagtga
      841 cgcgcgatgt cgatcatcaa accgcgccat gggaagcgtg gtcgatcgtc tatttgcacg
      901 ccgggcagga agtatccgtt gcgatcggcg tcgagtagtt gcaggacggt ttcgaggccg
      961 cggatggcgc cgattactgt gggcgcgctg agaatcgcct gacggttggt gatatcgatg
     1021 cgataacttt cgtcttcgcc gaggccggga atgtttttgc cgggaccctg cgtgtggata
     1081 atcaatgggg ttgtttgatc atcgagcgcg agtgccggag aaagtgtgaa gacggtgcgt
     1141 ccttcgagac gcttgatgaa ccgccagatc gccgcttgca gccgtgcgtc tgaatggccg
     1201 cgcgtggcga cgcggaagtt gctatcgacg gccaggcgct cgcgatggaa tatcactgac
     1261 gcgggcacgg gcatcaagtt gagcttcggc gttgatgctt cctgcgctga tactacgata
     1321 gtgcaagcga tgattagtaa cagagttttc atttgattcg tgttggtatt gatagttgta
     1381 gacaggatga caggatttac aagattgaca tgaatgaatc ctgttcattc tgtaaatcct
     1441 gtcatcctgt caaaggattt atttcactgt aaatctctgc atcaaaccag caatcctgat
     1501 ctcaaaatca ccaggctcga cgatgggctt attgtcagta ccgatgaacg acagatcttc
     1561 acggcgaagt ttaaaggtca ccgtccgact ttcaccgggt gcgagactca ccttcgcaaa
     1621 gcgtcgcaaa cgtttccccg gcggcgatag cgacgccacg agatcgctga catagaccag
     1681 cactgcttct ttccccgcgc gattgcccgt gttcttcaca gtgacactca ccggcaattc
     1741 ttcattcgcg gagattgtct gcttcccaag ccgcagatcg ctgtaactga aagtcgtata
     1801 gctcagcccg tcaccaaaat tgaactgcgg gttgaacgcc atgtttccaa acgaagtgtt
     1861 ctcagtctcg aatggcttgt gatcgtaatt gatcagacca ttcggcgtgc gcgggtaagt
     1921 gaagggaagc ctgccactcg gattaacgtc gccgaacaac acatcagcga tcgccgttcc
     1981 accttcatga ctcgggttgt aagccatcac gatcgcattc atgtcgtcag cgatacgatt
     2041 gatgatgcgc ggacgacctt cgacgagcac gagcacgatc ggtttgcccg tcgccttcaa
     2101 cgcttcggca agtttgattt gcggcgcacc gatcgtcaga tcggtaacgt tgccgggcgt
     2161 ttcgcaatac gagccttcac ccaacgccag cacgatcaca tcgacatttc gagcagcttg
     2221 aacggcggcc gcaatgtcga cctcctcttc ctgatccgtt ggtgtgctgt ttgacggccc
     2281 gggagctcgc gtgatcttcg tgcctggaac aaacgtgaca ttgccggcgc cgagcttcgc
     2341 ttcgagcgct ttacggatcg tgtgtcgatc tttcggatag aggttttctt ccgagccctg
     2401 ccaaacgtaa gtccagccgt tgttcatcga gaccagcgaa tccgcggttg gaccagtaac
     2461 gagaactttc gtcgtctttg caagcggcag gatattgttg ctgttcttta acagcgtgat
     2521 cgattcgcgc gcagcttcga gactgacctg tcgatattca ggcaagccaa acttcgactt
     2581 cagcgatgca tcgggcatcg gtttgtcgaa cagtccgagc tcaaacttga cccacagtat
     2641 ccgacgcact gcttcatcga tgcgcgactg cggtaccgcg ccttccttca cgagggcgat
     2701 caggtgatcg gcgaaactca gatcgttggg caccatgctc atatcaatgc ccgccattac
     2761 cgcgagacgc gtcgcttctt tttcatccgc agcgatgcgc cagacgttga cgagtttctt
     2821 gatgtcttgc cagtcagaga cgacgaagcc tttaaagccg agctccgtgc gcaggatgtc
     2881 agtgaggata tgtcgattga cgtgaccggg aacgccgttg atctcgcccg agttgaccat
     2941 gatcgtgcgg gcgcctgctt tgatcgcagt ctcgaacgtc gggagaaagt attcgcgcag
     3001 gtaattttcg ggaatccatg caggcgttcg atctcttccg gtgagtggaa aactgtagcc
     3061 catgtaatgc ttcaggctgg tcgcgaccgc gttcgatgac gagacgtccg tgccttccag
     3121 gccacgcaca aacgcaacac ccatcacctt tgcaagatac ggatcctcgc cgaacgtctc
     3181 ccagaaacgc ggccacagtg gattgcggcc gagatcgaga accggagaaa aactccacgg
     3241 tatgcctgcg gcgcgcgttt cgatcgcggt gatctccgag gcgcgcttca tcaattcggg
     3301 attgaacgtc gcggccatgc cgatctcttg cggaaagaga gtcgcgccca ggacgtagtt
     3361 ggcgccgtgg atcgagtcga tgccgtaaat catcggaatg ccgagacgcg ttttcttagt
     3421 cgcagcttcc tgaatctgcc cgatgatctg gtgccagtga tcgacagtga gcgcctggtc
     3481 ggcgacgttc agaaccgatc cgacgccgta cttgacgatc gctctttcga gttttgccgg
     3541 atcgaccttg atctcctgat cctttcccga cgtgacctga tcgatcgaga gctgggtcat
     3601 ctgcccgact ttttcttcga gcgtcatgcg cttgagcaga tcttcgatct tgcgcgagtg
     3661 atcgcgttcg ggaccgttgc ggctggcgcg ctggaagaat tcgtcgctat cgacgaggtt
     3721 ggatgtctgg gctttgatcg tgattagtgg ctgtacggcg aagcacagtg acagactgac
     3781 tgcccacagg gcgagtaggt gtttccgcat ctataagctg tcctcaggaa ggaaatttca
     3841 ggaacggggc tattttaact aattagccgg acttttcaat ggtcatcaaa caaag
//
LOCUS       DASUEX010000095         2516 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1613930, whole genome shotgun sequence.
ACCESSION   DASUEX010000095 DASUEX010000000
VERSION     DASUEX010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2516
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1613930"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2393)
                     /locus_tag="VFV61_01610"
     CDS             complement(<1..2393)
                     /locus_tag="VFV61_01610"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HEX4899196.1"
                     /translation="MVALILSSSALASAQNSKRSLSRVGLPTPSSVLGFAPGDDRTIA
                     DWRQITDYFARLDKASDRVLVRNIGPTTLGRPLIAAFISSRENIQALDKYQEIQRRLA
                     DPRTVPTDVARDALVTSGKVVVAISCSIHSTEIVASQMSMQLAYELASAQDQSTREIL
                     QNTILILIPSANPDGIDIVANWYRKTLGTKYEGQEPPELYHHYAGHDDNRDWFMLNLQ
                     ETRAISRLFWREWFPQIVYDIHQQGPNGSRLFIPPFFDPANPNIPPLLLRQVGLIGHK
                     IAADLQAAGVKGVVTNSTYDTWWHGGFRTAPYYHNSIGILSEAASAKLMTPTTVTADQ
                     LKRANRRGLRNALEASTSFPDPWPGGEWRARDIMNMEMISSRAVLSLAAKFRGEYLRN
                     FFELGRAAAMAGTANDPIAYLVQAGQGRDEAVAKLLDALISQGMEVYRLDRELHASFG
                     PQVLQRTNSAAEKIGTYRQIMNHTTGMHEVPLGSYIIFLNQPQRQNVLALFEPQIYPN
                     RIDGMGEVEQPYDVAGWTLPMQMGVEADGVMAIQEAASERRLTLLRDPNEVRKDLALP
                     LKNSDASPIENPLPRAVRIGLYKSGTANMDEGWTRFVFDTYNVPYTSLSNGDMQVEDR
                     LAQFNVIVLPSLRAREIPDGNTTSYPPEFAGRISEAGVARLKAFVTNGGTLICFDGSC
                     ELAIKQFNLPLRNALEGVKSSEFYCPGSVVALEVDTSQPLARGLRPVTDAYFINSSAF
                     ELTEDRTGENVRIVARYAKENVLRSGWLLGEARLRGKIALAQVQVGKGQIILFAFRP"
BASE COUNT          565 a          675 c          698 g          578 t
ORIGIN      
        1 tggggacgaa aggcaaaaag aattatttga cccttcccga cttgcacctg tgccagcgcg
       61 attttgcctc taagtctggc ctctcctaag agccatcccg aacgcaagac gttctctttc
      121 gcataacgcg cgactatccg cacgttttcc ccggttcgat cttcggtcag ctcaaaggct
      181 gagctgttga tgaagtaagc gtctgtgaca ggtcgcaaac cacgagccag gggctgggag
      241 gtgtctactt ccaaagccac cacggagccg gggcaataaa actcagatga tttaactccc
      301 tcaagcgcat tgcgcaacgg caggttgaat tgtttgatcg cgagttcaca ggagccatca
      361 aagcaaatca gggtgccgcc gtttgtgaca aaggccttga gtctagctac tcctgcttca
      421 cttattctgc cggcaaattc cggtgggtaa ctggttgtat tgccgtcagg gatctcgcgc
      481 gcgcgtaggg acggcaatac gatcacgttg aactgagcca agcgatcctc cacttgcatg
      541 tcgccgttgc tcagtgaagt gtagggcacg ttgtaggtat caaagacaaa gcgggtccag
      601 ccttcgtcca tattcgcagt ccccgacttg tagagcccaa tccgcaccgc gcgcggcaaa
      661 ggattttcaa tcggcgaggc atcgctgttc ttcagcggca gcgcgagatc tttgcggact
      721 tcattcggat cgcgcagcaa cgtaagacgg cgttcactcg ctgcttcctg aattgccata
      781 acgccgtctg cctcgacgcc catttgcatc ggcagagtcc acccagccac gtcgtaaggc
      841 tgttccactt cgcccattcc atcgatgcgg tttggataga tttgaggctc aaacaacgct
      901 aaaacattct gtctctgagg ctgattgaga aaaatgatgt aactgccaag cggcacctca
      961 tgcatgccag tggtgtggtt cattatttgg cgataagtac cgattttttc ggcagcggag
     1021 tttgttcgtt gcagcacctg cgggccaaac gaggcgtgca gttcgcgatc aagtcggtaa
     1081 acttccatgc cctggctgat caatgcgtcc agcagtttgg caacagcttc gtcgcggcct
     1141 tgacctgctt gcacgaggta ggcgatgggg tcattcgctg ttcccgccat tgccgcggcc
     1201 cgccccagtt caaaaaaatt ccttagatat tcgccgcgaa attttgcggc aagagaaaga
     1261 accgcgcgcg aggaaatcat ctccatgttc atgatgtcgc gagcacgcca ttcgccgccc
     1321 ggccagggat cgggaaagct tgtgctagct tccagagcat tgcgcaagcc gcgacggttt
     1381 gcccgtttca attgatcggc ggtcaccgtt gttggagtca tcaacttcgc actggccgcc
     1441 tcagagagga tgccaatcga gttgtgatag tagggagcgg tgcggaaacc gccatgccac
     1501 caggtgtcgt aggtcgagtt tgtgaccact cccttgacgc ctgccgcctg caaatccgca
     1561 gcaatcttgt gcccgattag tcccacttgt cgcagcaata aaggtggaat gtttggattt
     1621 gccggatcga aaaacggtgg aatgaaaagc ctcgagccgt tgggtccttg ctggtgaatg
     1681 tcgtagacga tctgcggaaa ccactctcgc caaaacaagc gcgaaattgc tcgagtctcc
     1741 tgcaaattca acatgaacca atctcgattg tcatcgtgcc cggcgtagtg atgatagagc
     1801 tccggtggtt cctgtccctc gtatttcgtg cccagggttt tgcgatacca gttcgcaaca
     1861 atgtcgatgc cgtcaggatt agcggaaggg atcaggatca gaatcgtgtt ttgcaggatc
     1921 tcgcgcgtcg actggtcttg cgccgaagcc aactcatagg cgagttgcat cgacatttgc
     1981 gacgccacaa tctcggtcga gtgtatggag caggaaatcg cgaccacaac cttgccgcta
     2041 gtgactaaag catcgcgtgc gacgtcggtc ggcaccgtcc gaggatcagc aagccggcgt
     2101 tgaatctcct ggtacttatc gagtgcctga atgttttcgc gcgagctgat aaaagccgcg
     2161 atcaaaggcc ggcctaaggt tgtggggccg atgttcctga ccaaaacgcg atcggaagcc
     2221 ttgtccaatc gggcaaaata gtctgtaatc tgacgccaat cagcgatggt ccgatcatct
     2281 cccggcgcaa atcccagcac tgaacttggc gtgggtaatc caactcggga aagactgcgc
     2341 ttggaattct gtgcgctggc gagtgccgag ctggagagaa tcagggcgac cacccagacg
     2401 atcgttaaaa gatgagttag taaggcgcgt ctgtttggtc tgatgggtcg caggaagttg
     2461 tgactccgga gttaaaattt gggcgagtct aacacaggtc agaacttccc atcggg
//
LOCUS       DASUEX010000096         4768 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_20665, whole genome shotgun sequence.
ACCESSION   DASUEX010000096 DASUEX010000000
VERSION     DASUEX010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4768
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20665"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="VFV61_01615"
     CDS             <1..279
                     /locus_tag="VFV61_01615"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX4899197.1"
                     /translation="RIAMFALGESHIELLEPLSPDSPVGKFLEKRGPGIHHIAVRVED
                     IRAKLAELKAKGLRLIDEVPRVGAGGCLVAFIHPTAANGVLLELVQHQ"
     gene            305..1603
                     /locus_tag="VFV61_01620"
     CDS             305..1603
                     /locus_tag="VFV61_01620"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX4899198.1"
                     /translation="MTTTKKAWIAAAVAVIFSLGLIVWQVKARRGEAVNLTAEDMALI
                     AEDQSPQFRARLASDEASRKDFADNIRSLFAVAEEARTKGIGNKPEVKRQIDLVRSIV
                     IAETFFKSEEGTAGAPQISEQEVEEFFKQPANKEKFDQFVADAKAKNPQMSGQITEEQ
                     MKQVRSQLGQVLIGESKGIAAGVDKKRSVQLQIQMEQARILAQTYAQEQLAEKMKATD
                     EEINAYIAAHPDLDVAKTREKAEAVLKRARGGEDFGKLAEEFSIDPGSKVKGGDLGWF
                     GPGAMLPEFEKAAFALSPGQISDLVETEYGFHIIKLEEKRTQEKDGKKEEQVHARHIL
                     IGGAQSDNPMAPPRSGRDQARAEVEKEKQKKVLDEIVKRSHVTVAENFTVKAPEMPQS
                     PAMPPGFHPQPAPAPESSAPAPKPDGAKSAPGKGAAKRNN"
     gene            1628..2467
                     /locus_tag="VFV61_01625"
     CDS             1628..2467
                     /locus_tag="VFV61_01625"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX4899199.1"
                     /translation="MQFGDYRVEIIPDTEFRLDGGAMFGVVPRNLWAKVCPPDEQNRI
                     RMNMNCLFIETATEKILIETGIGEKWSAKHSAMYGINRERSLADSLRAITGYGPEAIT
                     IVINTHLHFDHAGGNTILNDDGEAVPAFPNARYFLSQAEYEHAEQPSERDRASYFPEN
                     WRPAYNNGQVEMKPADYEVISGLRMETYPGHNRSMQCWRLEREGRTLFGFADLVPMRA
                     HVSLPWIMGYDLYPVETLESKKKLLPQAVRESWSCLFYHEPDRPLCKVIEDNGKLLAI
                     PTN"
     gene            2516..3403
                     /locus_tag="VFV61_01630"
     CDS             2516..3403
                     /locus_tag="VFV61_01630"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: protein motif:HMM:NF005657.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HEX4899200.1"
                     /translation="MKQASIGIIGGSGLYQMPELRDVEEVKVETPFGDPSDAFIIGTL
                     EDQRVAFLPRHGRGHRFTPTEVPYRANIYGMKLLGVERLLSASAVGSLQEQYAPLDMV
                     IPDQFFDRTRARARESTFFGEGIVAHVGFAHPVCGELGDVLERSCQAAEVKTHRGGTY
                     ICMEGPAFSTKAESNVYRSWGMDVIGMTNLQEAKLAREAEICYATLALVTDYDCWHPG
                     HDAVSVDTVVEYLNKNVRNAQIIMKAAVKDLSAAPRTCKCGSALRSAIFTAPDLWPAA
                     TTKRLAAIIKKYAASEIGS"
     gene            3416..3898
                     /gene="bamD"
                     /locus_tag="VFV61_01635"
     CDS             3416..3898
                     /gene="bamD"
                     /locus_tag="VFV61_01635"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEX4899201.1"
                     /translation="MKKSLSIVVLLLAIAICAPVVTAQVTVRGPDPSTLRDPELEKDS
                     MHNLEVARNYFKLKKAYVAALKRTEEIIAGNPNFTRIDEALFIAGSSSLSLAENKGKQ
                     TPSLYISYDGNTKKTLTAEEFREMGRDYLSRLVNDYPDSSFRGQAEEALKLVGGPKKQ
                     "
     gene            4005..>4768
                     /locus_tag="VFV61_01640"
     CDS             4005..>4768
                     /locus_tag="VFV61_01640"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX4899202.1"
                     /translation="MSLLVVGSVAFDAVETPFGKCEKMLGGSTSHVSLAASYFSDVRI
                     VGVVGGDFSDEERQVFVRHGVDTSDLECVPEGETFRWYGRYDYDLNTAHTLDTKLNVF
                     ADFKPKLSAASRESRLVFLGNIQPDLQREVREQVPQAELVALDTMNLWIDIARESLLQ
                     TIRGVDVVIINDAEARQLTGIPNVIKAAKTILTWGPKALIIKRGEYGAALFTPESYFA
                     IPAYPLESVFDPTGAGDSFAGGFMGYLSSQENLDNAA"
BASE COUNT         1141 a         1193 c         1411 g         1023 t
ORIGIN      
        1 cgcatagcga tgtttgcgct gggcgagtcg cacattgagt tgcttgagcc gctttcgccc
       61 gattcccctg tgggaaagtt tctggagaag cgtggcccgg gaattcatca tattgcggtc
      121 cgagtcgaag acattcgcgc caagctcgcc gaactgaaag ccaaaggcct gcgtttgatc
      181 gacgaagtgc ccagagtggg agctggcggt tgcctggtcg cgttcattca cccgacagcc
      241 gccaacggcg tgttgctgga attggtacaa catcaatagt tcgtatttgg aaaggaagtt
      301 tattttgaca actacaaaga aagcatggat tgcggcggcc gtcgcagtaa tcttttcact
      361 cggattaatt gtgtggcagg tgaaggctcg gcgcggcgag gctgttaacc tgactgcgga
      421 agacatggct ttgattgccg aggaccagtc gccgcagttt cgtgcccggc tggcgtcaga
      481 tgaagcgtcg cggaaggatt ttgcggacaa cattcgcagt ctgtttgcgg ttgcggaaga
      541 ggctcgcact aaaggcattg gcaacaagcc ggaggtcaaa cgccagatcg atctggtccg
      601 ttcgatcgtg attgccgaaa ccttcttcaa gtcggaggag ggtacggccg gggcccctca
      661 gatttctgaa caggaagtgg aagagttttt caaacagccg gccaataaag aaaagtttga
      721 tcagttcgtt gccgatgcca aggcgaagaa tccgcaaatg tccgggcaga ttactgagga
      781 gcagatgaaa caggtccgca gtcaactggg ccaggtcttg atcggcgagt caaaggggat
      841 tgcggcggga gtagacaaga agcgttcagt gcagttgcag atccagatgg agcaggcgcg
      901 catcctcgcc cagacctacg ctcaggagca gttggctgaa aagatgaaag ccacagatga
      961 ggaaatcaac gcctacatcg cggcgcaccc ggacctcgat gtcgcaaaga ctcgggaaaa
     1021 agccgaggca gtgctgaagc gggcgcgcgg tggcgaggat ttcggaaagc ttgcggaaga
     1081 gttttcaatt gatcctggca gcaaagtgaa aggcggcgat ctcggttggt ttggccccgg
     1141 ggcaatgctt cctgaattcg agaaggcagc cttcgctttg agtcccggtc agattagtga
     1201 cctggttgag actgaatatg gtttccacat aatcaagctg gaagagaagc ggactcagga
     1261 gaaagacgga aagaaagaag agcaagtaca cgcccggcac attttgattg gcggcgctca
     1321 gtcggacaat cctatggctc cgccgcgcag tggccgcgac caggcgcgcg ccgaagttga
     1381 gaaagaaaag cagaagaaag ttctggatga aatcgtgaag cgctcgcacg ttaccgtagc
     1441 ggaaaacttc acggtaaagg cgccggagat gccgcagtcg cctgcgatgc cgccgggatt
     1501 tcatccgcag ccggcgcccg caccggagtc gtcagcgccc gcgcctaagc ctgacggagc
     1561 gaagagcgcg cccggtaaag gcgccgccaa gcggaacaac tgatctgccg ggccgttaga
     1621 gtttcccatg cagtttggcg attatcgcgt cgagattatt cctgacacgg aatttcgtct
     1681 cgatggtgga gcgatgtttg gagtcgtccc gcgtaatctg tgggcaaaag tctgcccacc
     1741 tgacgagcag aaccgcatcc gcatgaacat gaactgtctc ttcattgaga cggcgacgga
     1801 gaagatactg atcgagaccg gcatcggtga aaagtggtcg gccaagcact ccgcgatgta
     1861 cggcatcaat cgcgaaaggt cactcgcgga ttcactgcgg gcaataacag gttacgggcc
     1921 cgaagcgatc acaatcgtga tcaatactca cttgcacttc gaccacgccg gtgggaacac
     1981 gatcctgaac gacgacggtg aagctgttcc tgcgtttccc aatgcgcgct attttctatc
     2041 tcaggctgag tacgaacacg cagaacagcc gagtgaacgt gaccgcgcca gctatttccc
     2101 ggagaactgg cgcccggctt acaacaacgg gcaggtggaa atgaagccgg cggactatga
     2161 agtgatttcc ggcctacgca tggagactta tcccggacac aatcgttcga tgcagtgctg
     2221 gcggttagaa cgggagggac gaacgctttt tggttttgcc gatctggtgc cgatgcgtgc
     2281 tcacgtttcg ttgccgtgga tcatgggcta cgacctttat ccggttgaaa ccctggagtc
     2341 caaaaagaaa ctattgccac aggcggttag ggaaagttgg agttgcctgt tttatcacga
     2401 accggatcga cccttgtgca aagtcattga agacaatgga aagttattgg caattccgac
     2461 aaattagaaa gcagggtaaa gaacagaaca attgcccgga aaagaaagag acgaagtgaa
     2521 acaagctagc atcggtatta ttggcggcag cgggctttac cagatgcccg aactccggga
     2581 cgtcgaagaa gtgaaggtgg agacgccgtt tggcgatccg tccgatgctt tcatcatcgg
     2641 cactttggag gatcagcgcg tcgcatttct tccccggcac ggtcgtggtc accgcttcac
     2701 tcccactgag gttccttacc gggcaaatat ctacggcatg aaactcttgg gcgtggaacg
     2761 tttgctctcg gcctcagcgg tgggttcctt gcaagagcaa tatgcgccac tcgacatggt
     2821 gattccggat cagtttttcg atcgcacgcg cgcacgtgcg cgggaatcca ctttcttcgg
     2881 cgaaggcatt gtcgctcatg tcggctttgc tcatcccgtg tgtggtgagt tgggcgacgt
     2941 cctggaacgc tcgtgccagg cggccgaggt caagacgcat cgcggcggga cctatatctg
     3001 catggagggt ccggcttttt caacgaaagc cgagtcaaat gtttatcgct cgtggggcat
     3061 ggacgtaatc ggtatgacga atctgcagga ggcgaagctg gcgcgggagg cggagatttg
     3121 ttacgccacg cttgctctcg ttaccgatta tgattgctgg catccgggcc acgacgccgt
     3181 ctccgttgac acggtcgtcg agtatctgaa taagaacgtg cgcaatgccc agatcattat
     3241 gaaagcagcg gtaaaggacc tgagtgcggc cccacgcacc tgcaaatgcg gctcggcact
     3301 acgcagtgcg atcttcactg ctcccgatct ttggccggcg gcgacaacaa aaaggctggc
     3361 tgccatcatc aaaaagtacg cagcatccga gattggtagt tgatgagagg ttgttatgaa
     3421 gaagtcatta tcgattgtgg ttcttttgtt agcgatcgcg atttgtgcgc cggttgtcac
     3481 ggcgcaagtc acggtaagag gaccggaccc ttcaaccctt cgcgacccgg agttagagaa
     3541 ggattcaatg cacaacctcg aggtcgcacg aaactacttt aagttgaaga aggcctatgt
     3601 tgcggcgctc aagcgtaccg aagaaatcat tgccggcaat cctaatttca ctcgtataga
     3661 tgaagccctg ttcatcgcgg gctccagcag tctttcactt gcggaaaaca agggtaagca
     3721 aaccccgtcc ctgtatatca gctatgacgg gaacaccaag aaaaccttaa ctgcagaaga
     3781 gtttcgggag atggggcgcg attatcttag ccgcttggtc aacgattacc cggacagcag
     3841 ttttcggggg caggcggaag aagcattgaa gttggttggc gggcccaaaa agcagtaggc
     3901 agtagacagc aggaagaagg cagtaggcgg aagcagttca agtttcgaaa ttcaaagttc
     3961 aaagaccgaa attcgaagac caaagttcaa agttcaaaaa atccatgtct ctcttagtcg
     4021 taggttcagt tgcgttcgac gcggtcgaaa cgccttttgg taagtgtgaa aaaatgttgg
     4081 gtggctctac ctcgcatgtg tcgctcgcgg ccagctactt ttctgacgtc aggatcgtcg
     4141 gtgttgtcgg cggcgatttc agcgacgaag agcgtcaggt gttcgtccgt cacggtgttg
     4201 ataccagcga tctggaatgc gtgcccgaag gggaaacgtt tcgctggtat ggccgttatg
     4261 actacgacct gaataccgcg catacgctcg acaccaaact aaatgtcttc gcggatttca
     4321 agcccaaact ttcggcggca tcaagagagt ctcggttggt ttttctcggc aatatccaac
     4381 cagatctcca gcgcgaagtt cgtgagcagg tgccgcaggc tgaactcgtg gcgctcgaca
     4441 cgatgaatct gtggatcgat atcgccaggg agtcgttgtt gcaaacgatc cgtggggtcg
     4501 atgttgtcat catcaacgat gctgaggcgc gccagttaac cggaattccc aatgtcatca
     4561 aggcagcgaa aaccattctc acgtggggac ccaaggcatt aattatcaaa cgcggcgaat
     4621 acggcgcggc cctgttcact ccagaaagct acttcgccat ccctgcgtat ccgctcgagt
     4681 cggtttttga tcccacggga gcgggcgact ctttcgccgg cggtttcatg ggctacttgt
     4741 cgagtcagga aaaccttgat aatgccgc
//
LOCUS       DASUEX010000097         6541 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_21152, whole genome shotgun sequence.
ACCESSION   DASUEX010000097 DASUEX010000000
VERSION     DASUEX010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6541
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21152"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="VFV61_01645"
     CDS             <1..293
                     /locus_tag="VFV61_01645"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEX4899203.1"
                     /translation="AAVAKLHFPGLHPDTARWLVTNRSIKAIGLDTASIDFGQSIKFE
                     SHQILFEKDIPAFENVANLDKLPARGFSVVALPMKIKGGSGGPLRIIALLNP"
     gene            303..965
                     /gene="modB"
                     /locus_tag="VFV61_01650"
     CDS             303..965
                     /gene="modB"
                     /locus_tag="VFV61_01650"
                     /inference="COORDINATES: protein motif:HMM:TIGR02141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HEX4899204.1"
                     /translation="MIDWFPIRLSFAVASAATLIALVAGSAIAWLLARKRFPGRDLLD
                     ALITLPLVLPPTVLGYYLLVLLGTRSPLGGFLYNNFGIRLTFTVTAAIIAATIHALPL
                     VTKSLRAAFEGVNIELESAARTLGLSNRLIFFRVTLPLASRGVLAATALAFARALGDF
                     GVTIMIAGNIPGKTQTASVAIYDAAQAGRDNEAFMLAVIVSLIAMVMLYFINHFGQKN
                     RT"
     gene            complement(999..2261)
                     /locus_tag="VFV61_01655"
     CDS             complement(999..2261)
                     /locus_tag="VFV61_01655"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX4899205.1"
                     /translation="MHRRFFIKSGSIALASIGVSLSAPSFLQRALLAQSQERLTGGRR
                     KTLIAIFQRGAVDGLNMIVPYGERNYYELRPSIAIPKPAAGNAEAALDLDGFFGLHPA
                     MASLKPFWERKQLAIVHASGSPDNTRSHFDAQDYMESATPGRKGTPDGWLNRYLQSKT
                     DSHRSLFRAVSMTQTMPRILQGSAPTLAISNLNDFAIRAGRSSANVQGGFEAIYEQTV
                     NDVLGGTGKETFAAVNYLKQVNPGQYQPENGAVYPRTAFGNSLFQIGQLLKAQVGLEV
                     AFTDIGGWDTHVNQGNARGQLALRLQDLSNGIAALATDLGKRMDDVVILTMSEFGRTV
                     RENGNRGTDHGHANAMLMIGNNVQGGKIHGAWPGLENDQLYEGRDLALTTDFRDVFAE
                     VAKKHLGTKNLQAVFPGYSISKSLNLVL"
     gene            complement(2387..4510)
                     /locus_tag="VFV61_01660"
     CDS             complement(2387..4510)
                     /locus_tag="VFV61_01660"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HEX4899206.1"
                     /translation="MQLSLYIIRPVAVLLFVALMVNGFTTVTRAGLDQNGKPSNSLTE
                     EQRITHALNRLGFGARPGDVERVKRIGIQKYIEQQLNPSQVSDEVAEAKVRNLATLTM
                     TTAQLYEKYPQPGQILRQLQRRGELPNDPANPRANQDPQTPPERTIEPEAMPRPEQPN
                     GNPNAAGRNEENRRAIRDYYAERGLLPPQRIMVELQASRILRATYSERQLQEVLVDFW
                     TNHFNVFSGKGADRWLLVSYDRDTIRPHTLGKFHDLLLATAQSPAMLFYLDNFQSVSP
                     NMQVAQRRRQDNGSAAGLFDFGRGTRRGLRRDQSQPGRNPGAAPEPAALPQPPASARL
                     QRRGINENYARELMELHTLGVDGGYTQKDVQEVARCFTGWTIFAPRGGGAAGAMMNQR
                     RAEMLRENAGRFFFNQRAHDDGEKTVLGHKIPAGGGIEDGLRVLDILAHHPATAKFIA
                     SKLTRYFVSDTPPPALVDRVAAAFTKSDGDISETLRAIFFSPEFNSPAAYRAKVKRPF
                     ELAISSLRTLGAETSGGPQLHQWIARMGEPLYGFQTPNGYSDSAESWVNTGGLLERLN
                     FALALASNRIPGTRVDLKRFAGNGAGGANVAKEKTMDQFLELIVGGDVSPKTRDLLLR
                     QLEQEVVITPAPARSQMAADESDGIVEPPRAMPGEMARERSEIPAGPESRGRGRPLAR
                     LEASITDPVTKIVGLILGSPEFQRQ"
     gene            complement(4776..6230)
                     /locus_tag="VFV61_01665"
     CDS             complement(4776..6230)
                     /locus_tag="VFV61_01665"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HEX4899207.1"
                     /translation="MRSTLRQTITLLSALLLIVLTNTSPGISQQVKPSRTGKRAGSYP
                     ASRNREIPRFIRDIDSRNIERTIRKLVSFGTRSTLSAQDDPNRGIGAARDFLYQEFVK
                     VAEKSGGRMTVEKQSFLQEKAARVPTPTVITNVVATLKGTQPQSEARVYVVSGHYDSM
                     CSSPTDEKCDAPGANDDASGTAAVLEMARVMAPYRFDATIVFMAVAGEEQGLLGSTYF
                     AEQARQKNTDIEAMFTNDIVGNTLGGNGVRDRRTVRVFSEGVPSSEKPEEAIVRRGVG
                     GENDSTSRQLARFIKQVAERYVPYMRVMLVYRRDRYLRGGDHIPFVERGYPAVRFTEP
                     NEDYTHQHQTLRTENGIKYGDLPEHVDFVYVADVARVNAASLGMLALAPAKPKGVSIL
                     TARLTNDTDLKWDANTEPDLAGYEIVWRDTTSPVWTDSRWIGRETAYTMTGMSKDNYF
                     FGVRAVDRGGNRSPVSFPRPLGRTGPAPSPTPRP"
     gene            complement(6271..>6541)
                     /locus_tag="VFV61_01670"
     CDS             complement(6271..>6541)
                     /locus_tag="VFV61_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899208.1"
                     /translation="MGVHPAFITWIVLFSMLTLFGSTALLMRFWLKILNLQRQHPAPR
                     PSITHSPPTAFVPQQLPPRYDPASSVTEHTTRTFSPIYREPSEPT"
BASE COUNT         1328 a         1859 c         1811 g         1543 t
ORIGIN      
        1 aggcggcagt cgctaagcta cattttccgg gcctgcatcc cgacactgct cgctggctcg
       61 ttaccaaccg ttccattaaa gcgattggac tcgacaccgc cagcatcgac tttggacaat
      121 cgatcaaatt cgagagtcat cagattcttt tcgagaagga catacccgct tttgaaaacg
      181 tcgccaatct cgacaaactt ccggcgcgag gattttcggt agttgccttg ccgatgaaga
      241 tcaagggcgg cagcggtggc cccttgcgca tcattgcgct gctaaaccct tagcttctcc
      301 ggatgatcga ctggtttcca atccgccttt cattcgccgt ggccagcgcc gcgaccctca
      361 ttgccctcgt cgctggttcg gcgatcgcct ggctgctcgc gcgcaaacgc tttcccgggc
      421 gggatttgct ggacgcattg atcacgctcc cgctcgtgct gccgccgacc gtgctgggct
      481 attacctgct tgtacttctc ggaacgcgct cgcctttggg cgggtttctc tacaacaatt
      541 ttggcatcag gttgacgttc accgtaaccg ccgcaatcat cgcggcgaca attcacgctc
      601 tgccgcttgt gactaagagt ttgcgcgcgg cttttgaggg agtgaatatt gaactggaat
      661 ctgcggcgcg gacactaggc ctgagcaacc gtctgatttt ctttcgggtg actttgccgc
      721 tggcatcacg cggcgtgttg gcggccactg ccctggcctt cgcacgcgca ctcggcgact
      781 ttggtgtgac gatcatgatc gccggaaata ttcccggcaa aacgcaaaca gcgtcagtgg
      841 cgatttatga tgcggcgcag gcgggacgag ataatgaggc tttcatgctc gcggtgattg
      901 tttcgctgat cgcaatggtg atgctttact tcatcaacca tttcggacag aagaacagaa
      961 cttagaacct gcaaagttcc aagttccaag ttcaaagttc aaagcactaa gttcaaagat
     1021 ttcgaaattg aataccctgg aaatactgct tgaagattct ttgtgccgag atgtttcttc
     1081 gctacttccg caaacacgtc gcgaaaatcc gtggtcaatg cgaggtcgcg accttcgtat
     1141 aactgatcat tctctaagcc aggccacgcg ccgtggatct taccgccttg cacgttgttg
     1201 ccaatcatta gcatcgcatt cgcatgaccg tgatcagttc cccggttacc gttctcacgt
     1261 actgtgcggc caaactctga catcgtaagg atcaccacat catccatacg cttccccaga
     1321 tcagtcgcga gggccgcgat gccgttgctt aagtcctgca atcgcagcgc gagttggccc
     1381 cgcgcattac cttgattaac gtgcgtgtcc cagccgccaa tatccgtgaa tgctacttcc
     1441 aggccaactt gcgctttcaa caactgcccg atttgaaaga gtgagttacc aaaggcagtg
     1501 cgtggataca cggcgccgtt ctcaggttga tactgccccg gattgacttg cttcagatag
     1561 ttaacggctg caaatgtttc cttgccggta ccgccaagca cgtcattcac agtctgctcg
     1621 taaatggctt caaatccgcc ttgcacattc gctgaagacc ttccggcgcg aattgcaaaa
     1681 tcgttcagat tggaaatcgc caatgtaggc gcgcttccct gaaggatgcg cggcatggtc
     1741 tgtgtcattg agacggcgcg aaagagtgag cggtgcgaat cggtcttgct ttgtagatac
     1801 cggttgagcc agccgtcggg agttcccttg cgaccgggag tcgctgactc catgtaatcc
     1861 tgcgcgtcga agtgcgaccg agtgttatcc ggcgaacctg aggcgtgcac aatcgcgagc
     1921 tgctttcgtt cccagaatgg cttgagcgaa gccatcgccg gatgcaggcc gaagaagccg
     1981 tcaaggtcga gcgcggcttc cgcgtttccg gctgctggtt tgggaatggc aatcgaaggc
     2041 cgcagctcgt aatagttacg ttcgccatag ggaacgatca tgttcagccc atccacggcg
     2101 ccgcgctgaa aaattgcaat cagagtcttg cgccgtccac ctgtgagtcg ctcctgcgat
     2161 tgtgccagta aggcgcgctg taggaacgaa ggggccgaga gtgaaactcc aatgctcgcc
     2221 agggcgatac ttcccgattt aatgaaaaag cgtctgtgca taatgcaact ccgaaggagt
     2281 cagtagtcag tagactgtgc cgtttctcgg ttgtcagttg ttagttgtta gttgtcgctg
     2341 agcccatcga cccaactgcg tcctgccttc tgctcctgcc tacctcctac tgccgttgaa
     2401 actccggtga acccagaatc aatcccacga tcttggtgac aggatcggtg atgctcgctt
     2461 cgagacgcgc taggggccgt ccgcgtcctc gggattctgg tccagccggt atttcggatc
     2521 gctctcgcgc catttcgcca ggcatcgctc gcggtggctc gacaattcca tcagattcat
     2581 ccgcggccat ctgacttcgc gccggagccg gagtaattac cacctcctgt tcgagctgcc
     2641 ttaacagcag atcccgtgtc ttgggcgaaa cgtcaccgcc gacgatgagt tccaggaact
     2701 gatccatcgt tttctcttta gcgacgtttg cgccaccggc gccgtttccg gcaaagcgtt
     2761 tgagatccac gcgtgtcccc gggatgcgat tgctggcgag ggccaaagca aaattcaagc
     2821 gctccagcaa tccgcccgtg ttaacccaac tctcggcgct gtccgaatag ccgttggggg
     2881 tttgaaagcc gtatagcggt tcgcccattc gcgcaatcca ctgatgcaac tgcgggcctc
     2941 cactggtctc cgcccccagc gtccgtagcg agctgattgc cagttcaaat ggtcgcttca
     3001 ctttggcccg atacgccgcc ggtgagttga actcaggcga aaagaagatc gcgcgcaaag
     3061 tttcgctaat gtcaccatca ctcttagtga acgccgcggc cacgcgatcc acgagcgcgg
     3121 gcggcggtgt gtcgctgaca aaatatcgag tcagtttgct ggcgataaac tttgcagtcg
     3181 caggatggtg cgccagaatg tccagcacgc gcaggccatc ttcaatgcct ccgcccgcag
     3241 gaatcttatg acccagcact gttttctcgc cgtcatcatg ggctcgttga ttgaaaaaga
     3301 accggcctgc gttttcgcgt agcatctctg cccggcgctg gttcatcatc gcgccggcag
     3361 cgccgccgcc gcggggcgca aagattgtcc agccggtgaa gcagcgcgca acttcctgca
     3421 catctttctg tgtatagcca ccgtcaacac cgagggtgtg taattccatc aactcccgcg
     3481 cgtagttttc attgatcccg cgacgttgca atcgcgcact tgcgggaggc tgcggcagag
     3541 cagctggctc gggcgcagcg ccgggattgc gacccggttg actttgatca cgacgaagac
     3601 cacgacgagt acctcttcca aagtcaaaca ggcccgcggc cgacccgttg tcctgtctac
     3661 gccgctgagc tacctgcatg ttgggactta cgctttgaaa gttgtctaga tagaagagca
     3721 ttgccggact ctgggccgtc gccagcaaca gatcatgaaa ctttcctaga gtgtgcggtc
     3781 gaatagtgtc gcgatcatag gaaaccaaca accagcgatc agcccccttg ccggaaaaga
     3841 cattgaagtg gttagtccag aaatcaacca gcacttcttg aagttgccgc tcgctgtaag
     3901 ttgcccgcag aattcgtgag gcttgcaact ccaccatgat gcgctgcggt ggcaagagtc
     3961 cgcgctcagc atagtagtct cgaatcgcac ggcgattttc ttcgttgcgg ccggcggcgt
     4021 tgggattgcc gttcggttgc tcgggtctgg gcatcgcttc gggttcgatg gttctttcgg
     4081 gaggagtttg agggtcctga ttggcgcgcg ggttggccgg gtcgtttggc agttcacctc
     4141 gacgttgcag ttgccgcaga atctgacccg gctgcggata cttctcatag agctgcgccg
     4201 tcgtcatcgt tagcgtggca aggttcctca ccttcgcctc ggcaacttca tcagagactt
     4261 gcgacgggtt gagttgttgt tcgatgtact tctgaattcc gatacgcttc acgcgctcta
     4321 cgtctccggg acgcgcgcca aatccgagcc ggttcagcgc gtgagtgatg cgctgctctt
     4381 cagtgaggct attgcttggc ttgccgtttt gatcgagacc cgcacgagtg actgtcgtga
     4441 agccgttaac cattaatgcg acaaataaca gaaccgcgac agggcgaata atgtaaagcg
     4501 atagttgcat tctcacatca gttcctttgg gttttcctgg atactggggc ttgatgccct
     4561 caagatttag acgcttggtg tgctttggcg gttgacaaac ttacttaaga gttgctgcta
     4621 ctacttgatg acggctttgt cgccggatgt gagcagcaag ttcagttcgg ggaagagttc
     4681 cgttcatgag taacgggccg cagccccatt gaaagaagta gtgctacggc gcggcaaagc
     4741 cgcgtctcag atccggcgat aaagcggttc gtatcctaag gtcgtggagt tggtgagggc
     4801 gcgggtcccg tccggcctag cgggcgcggg aagcttaccg ggctgcgatt ccctccccga
     4861 tcaaccgcgc gcaccccaaa gaagtagttg tctttggaca ttcccgtcat cgtgtatgcc
     4921 gtttctcttc cgatccagcg tgagtctgtc cagacaggtg aggtggtatc gcgccagaca
     4981 atttcgtagc ccgccaaatc aggttcggtg ttcgcgtccc acttgagatc agtgtcattt
     5041 gtaagtcgtg cggtcagaat gctgacgcct ttcggttttg cgggcgcgag tgccagcatt
     5101 cccagactcg cggcgttgac ccgagcaacg tccgccacgt agacgaagtc cacatgttca
     5161 ggcaagtctc catacttaat gccgttctcg gtgcggagtg tttgatgttg atgggtgtag
     5221 tcctcgttcg gctcagtgaa tcgcacagcc ggataacccc gttccacaaa aggaatatga
     5281 tcgccgcccc ttaagtagcg atcacggcga tacaccagca tcactctcat gtaaggtaca
     5341 taacgttccg ccacctgctt gatgaagcgt gcgagttgcc gcgaggttga gtcgttctcg
     5401 ccgccgacgc cgcgtcgcac aatagcttcc tcaggtttct cgctcgacgg cacgccctcg
     5461 gaaaacactc gcaccgtgcg ccggtcgcgt acaccattgc cgccgagagt gttgccgacg
     5521 atgtcgttcg tgaacatcgc ctcgatgtcg gtgttctttt gcctcgcttg ttcggcaaaa
     5581 tacgttgagc ccagcaaacc ttgctcttca cccgcaactg ccatgaacac aatagtggcg
     5641 tcaaacctgt agggcgccat cacgcgcgcc atctccaata cggccgctgt tcctgaggcg
     5701 tcgtcattgg ccccaggggc gtcacacttc tcatctgtgg ggctggagca catcgaatcg
     5761 taatgaccgc taaccacgta gacgcgcgct tccgactgcg gttgcgtccc cttaagcgtc
     5821 gcgaccacat tggtaatcac agttggcgtg ggaacgcgtg cggctttctc ctgaagaaaa
     5881 ctctgctttt caacggtcat gcgcccaccg gatttttcgg caaccttcac aaactcctgg
     5941 tatagaaaat cacgcgcggc gccgatgccg cgattgggat catcctgtgc cgacagagtg
     6001 ctgcgcgtgc caaaggaaac gagtttgcga atcgtgcgtt cgatgttgcg gctatcgatg
     6061 tctctgatga atctgggaat ctcacgattc cgactagccg gataactccc agctcgcttc
     6121 cccgttcggg atggttttac ttgttgtgat atccccggtg aagtgtttgt caaaacgatc
     6181 aagagcaatg ctgacagcaa agtgatcgtc tgcctgagcg ttgatctcat tgcaacctcg
     6241 cagtctgatg agcagacatt gtaatggggc ctaagtcggc tctgaaggct cgcgatagat
     6301 cggcgagaac gttcgcgtgg tgtgctcggt aacgctcgac gccggatcgt agcgcggcgg
     6361 tagttgttgc ggcacaaacg cagtcggcgg tgaatgggtg atgcttggtc gcggcgcggg
     6421 gtgctgtcgc tgcaggttca gaatcttcaa ccagaaccgc attagcaagg cggtgctgcc
     6481 aaagagggtc aacatgctaa acaaaacaat ccaggtaata aaggcaggat gaacacccat
     6541 c
//
LOCUS       DASUEX010000098         2107 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1052221, whole genome shotgun sequence.
ACCESSION   DASUEX010000098 DASUEX010000000
VERSION     DASUEX010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1052221"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(157..933)
                     /locus_tag="VFV61_01675"
     CDS             complement(157..933)
                     /locus_tag="VFV61_01675"
                     /EC_number="3.1.4.17"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004114 - 3',5'-cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-nucleotide phosphodiesterase"
                     /protein_id="HEX4899209.1"
                     /translation="MKIQLLPSTFDGDGQATMDQRLTCYLIDDCVTLDAGSIAIALTA
                     EQRASVQDIIVTHVHMDHIASLPIFIDDLYPTLTQPMRIHATPDVIERLERDVFNWNV
                     YPRFSELKNEFGAVMEYVPIHPGQEFKVAHLTVQAIPVNHIVPTVGIVMSDGRRTVAF
                     SSDTAETDEFWEVLNGLPRLDAILIEASFPNAMAGLADVSRHFTPASLATELRKLKHN
                     GLDILAVHLKPAYRETIIAELVALGLPKLDVMEPGKTYSW"
     gene            complement(1044..>2107)
                     /locus_tag="VFV61_01680"
     CDS             complement(1044..>2107)
                     /locus_tag="VFV61_01680"
                     /inference="COORDINATES: protein motif:HMM:NF024760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HEX4899210.1"
                     /translation="IVAVLIAILLGLIVLLLIGFETGPVGLMIGMVAATLPVPIYVGL
                     ALWIDRYESEPLWMLATAFFWGALVAVFIAFLFNTASSVMVAVVTESQEAGEAFGAVV
                     SAPIVEESAKAIILFIFFFAKRDEFDGVVDGIVYAAMVGLGFAMTENIQYYGRAAFAE
                     GPGLTGVFILRGTLAPFSHPLFTSLTGIGLGLARQSRNIFVKLIMPVLGFAMAIALHS
                     TWNGSIVIFGGAGFLVTYVLIMVPVFAIILVIILLALRREGQIVRQFLVTDMHGGVFT
                     AEEYNRLCTIRGRMGANFNAFSRGGLGHWRASRQFHQTASELAFHRSRIARGMSSADA
                     QEREAAYQMALRDLLQRLR"
BASE COUNT          511 a          574 c          573 g          449 t
ORIGIN      
        1 atgacggtaa cgttccgcgc ggagctgatg cctggtcgtg accgcgaggc gcgcaccttt
       61 cgcgtaaaag aattgttatc tagtgggcgt gtaacgcttg acggcgttgc gggtgagcac
      121 gcggaaaaag aatttaatcc aatccgctag caggtcctac cagctataag tctttccagg
      181 ttccatcaca tcaagtttgg gcaagccgag ggctacgagt tccgcgatga tcgtttcgcg
      241 gtacgcaggt tttaagtgca ctgccagaat atcgagaccg ttgtgcttga gtttgcgcaa
      301 ctccgttgcg agtgaagccg gagtgaagtg acgagagacg tctgctaacc cggccatcgc
      361 attcgggaac gaagcttcga tcaagatcgc gtccagtcgg ggcaggccgt tcagcacttc
      421 ccagaattca tctgtttccg cagtgtccga actaaaagca accgtgcgcc ggccatccga
      481 cattacgatg cccacggtgg gaactatgtg gttcaccggg atggcctgca ctgtcagatg
      541 tgcgacttta aactcctgcc ccggatgaat cggcacatac tccatcacgg cgccaaattc
      601 attcttcagt tctgaaaagc gcgggtagac gttccaatta aagacatcgc gttcgaggcg
      661 ctcgatcacg tcgggtgttg catgaatgcg catcggctga gtcagcgtcg ggtaaagatc
      721 gtcgatgaag attggcaggc tggcgatgtg atccatgtgc acgtgcgtca caatgatgtc
      781 ttgcacactg gcgcgctgct cagccgtgag cgcgatcgca atgctaccag catccagcgt
      841 gacgcagtcg tcgatgagat agcaggtgag gcgttgatcc atagtcgcct ggccgtcgcc
      901 atcgaaggtg ctgggtagga gttggatttt cacgggctgt ttcggcgaaa agaactcttc
      961 gcgcggtccg tccttagtat aagcgaagtg tcttaaatct cgcgagactt ttaacctgta
     1021 tagcgctggg tcccggcgtc aaatcagcgc agtctttgta acagatctcg caaagccatt
     1081 tgataagctg cttcacgctc ctgcgcgtcc gcactcgaca ttccgcgcgc aatgcgactg
     1141 cggtggaaag ccagttcgct ggcggtttgg tgaaactgcc ggctagcgcg ccaatgtccc
     1201 aaaccgcccc ggctaaaggc attgaagttg gctcccatcc gtccacgaat ggtgcagaga
     1261 cgattgtatt cttcggctgt gaagactccg ccgtgcatat ccgtaaccag gaactgacga
     1321 acaatttgtc cttcgcgtcg cagcgcaaga agaattatga caagaataat agcgaatacc
     1381 ggcaccatga tgagcacgta ggttacgaga aagccagcgc cgccaaaaat cacgatcgag
     1441 ccattccagg tactgtggag agcgatggcc attgcaaatc ccaggacggg catgatcagt
     1501 ttgacgaaga tatttcttga ttgccgggcg aggccgaggc cgattcccgt aagactcgtg
     1561 aagagtgggt gcgaaaacgg agccagcgtg ccgcgcagta tgaagactcc agttaatccc
     1621 ggcccttcag cgaatgcagc ccgaccgtag tattggatat tctccgtcat tgcgaaaccg
     1681 agtcccacca ttgccgcgta gacaatcccg tccaccacgc cgtcaaattc atcccgcttg
     1741 gcaaaaaaga agatgaaaag gattatggct ttggcgcttt cctcgacgat cggagcagaa
     1801 acaacggcgc caaacgcttc gcctgcttcc tggctttccg tcaccactgc caccatgacg
     1861 ctgctcgccg tattaaataa gaaggcgata aagacggcta cgagtgcgcc ccaaaagaac
     1921 gctgttgcca gcatccaaag cggctctgat tcataacgat caatccaaag cgcgagtcca
     1981 acatagatag gaaccggcaa ggttgcggcc accatgccga tcattaatcc tactggcccg
     2041 gtctcgaatc caatcagcag caatacgatc aatcccaaca ggatagcaat caacaccgcg
     2101 acgattg
//
LOCUS       DASUEX010000099         5572 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_823040, whole genome shotgun sequence.
ACCESSION   DASUEX010000099 DASUEX010000000
VERSION     DASUEX010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5572
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_823040"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(76..864)
                     /locus_tag="VFV61_01685"
     CDS             complement(76..864)
                     /locus_tag="VFV61_01685"
                     /inference="COORDINATES: protein motif:HMM:NF012995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HEX4899211.1"
                     /translation="MSQTTRYLKRINYSGPTTPTAETLRQLHVAHLQTVPFENLSIHM
                     GEPIVLEDQALFEKIVVRRRGGFCYELNGLFASLLRTLGFGVVRLSARVANNQGEFGP
                     EFDHMTLLVTAPDAPQIRWLADVGFGDSFNEPLLLDSRESQQEGLRAYRIDAEKDHLQ
                     LLQREGEGEWKPQYRFTLEPHEYSDYLQMCHYHQTSPQSHFTKARVCTRATPEGRITL
                     SELRFITTANGERHERSLDDEDAYRLVLREQFGIEINRWRPLSV"
     gene            complement(916..1746)
                     /locus_tag="VFV61_01690"
     CDS             complement(916..1746)
                     /locus_tag="VFV61_01690"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HEX4899212.1"
                     /translation="MTNKQISAVATFHALHESGCFVLPNPWDIGTAVYLQHLGFKALA
                     TTSAGYAFTLGLPDSVLAIKRGEMLGHVAEIAAATPLPVNADFQNGYADSPAEVAASV
                     SLCIETGVAGLSIEDNTGRANSPLYETSLALERIKAAREAIDKSGIPVVLTARCEAAL
                     VGHATPLKVALERLTAFADAGADCLYAPGLAEPEQIKAIVKVVAPKPVNVLMSRPNPT
                     MTVTRLADLGVRRISVGSALARVAWGGFIRAAKQLAEGSFEGFAGAASIKELDQVFDG
                     "
     gene            complement(1802..3559)
                     /locus_tag="VFV61_01695"
     CDS             complement(1802..3559)
                     /locus_tag="VFV61_01695"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEX4899213.1"
                     /translation="MTSNYARSFVALSLFLLISLAFPQKAKLENESQANLRFAISFAT
                     ERSNTPLDGRLLLLISKNNESEPRFQINEDLNTQQVFGTDVEGLKPDAEAIVDASAFG
                     YPVRSLAQLPPGEYWIQALLHRYETFKRSDGHTVKLPMDRGEGQQWSRAPGNLYSKPQ
                     KLAIDPQKTETIKLKLDQEIPPIAPPQDTKYIKHVRIQSKLLSDFWGRPMHLGANILL
                     PEGFDSHPNARYPLVIFHGHFPADFGGFRETPPDPNLKPDYSERFKLAGYNRIVQEHA
                     YKFYQEWTGPNFPRMIIIEIQHANPYYDDSYAVNSANLGPYGDAITYELVPYIEKQFR
                     GIGQGWARFMYGGSTGGWEALAAQIFYPDEYNGAWGACPDPIDFRAYTVVNIYEHKNA
                     YYADSPWKKVPRPGKRNYLGELAATTEDENHMELALGTKSRSGGQFDIWEAVYSPVGP
                     DGYPKPIWNKLTGEIDHSVAAYWREHYDLGHILKRDWAKLGPKLEGKLHIYVGEADNY
                     FLNNAVYLVEDFLKSTKNPHYNGEVDYEPRAEHCWNGDHTRPNATSRLRYHQFYAPKI
                     VERILKSAPPGADLKSWRY"
     gene            complement(3873..>5572)
                     /locus_tag="VFV61_01700"
     CDS             complement(3873..>5572)
                     /locus_tag="VFV61_01700"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4899214.1"
                     /translation="ILQPKFTQYAIDWYILPRSADGLMLLVGLFAGVQFLRLIFSYLQ
                     DLLLKTVGQYVMFDMRRELYDKLQHQEVAYYDRNPVGRIMTRLTNDVDALNELFTQGV
                     TDVLGDLVMIFAIIGVMLWMDVRLTLVTLLTVPLLFAATTWFRKGARRGYDMVRTRIA
                     RINAFLQEHFAGAQTVQIFNAESKSLGKFDDINDDYRKANLDTIFYYALFFPLVDFIG
                     AVGIALVIWYGGYRVMNSALTLGALVAFIQYSGFLFQPIRDISDKYNVLQAAVVASHR
                     IFKTLDLPIAITSPAEPLRNERARGLIEFRDVWFAYKDEDWVLKGVSFTVNPGESVAL
                     VGHTGSGKTTITNLLMRFYDIQRGQILLDGVDLRDWDLESLRRNFAVVLQDIFLFSGT
                     VESNIRLGRDDISDERVHWAAQEVHAHRFIERLRDGYKSEVKERGAGLSVGQKQLISF
                     ARALAFDPALLILDEATSSIDTETEQSIQQAIGRVMQDRTSIVVAHRLSTIQRADQII
                     VLHHGEIREQGRHQELLAQHGLYWMLYKLQYADGAPPQLMIGRDEDATTQPISDFAQ"
BASE COUNT         1291 a         1514 c         1477 g         1290 t
ORIGIN      
        1 gacacccgaa agcgacctct ttcctacttc ggactgaccg cgggggtgtc ctaaacttca
       61 gttttcctga ttagttcaaa cactgagcgg ccgccaacgg tttatctcga ttccaaactg
      121 ttcccgtagc acaagccgat aagcatcctc gtcgtccagg cttcgttcat gccgttcgcc
      181 gttagcagtg gtaataaatc gcagctcact caatgtaatg cgtccttcgg gcgtggctcg
      241 ggtgcagact cgagctttag taaagtgtga ctgcggcgaa gtctgatggt aatggcacat
      301 ctgaaggtag tcagaatatt cgtgaggttc gagcgtaaaa cgatactgcg gtttccattc
      361 accttcgccc tcgcgttgta agagctgcaa atgatctttc tccgcatcga tccggtatgc
      421 gcgtaaacct tcttgctgtg actcacgaga atcgagcagc aatggttcgt taaatgaatc
      481 tccaaagcct acatctgcga gccagcgtat ttgcggggcg tccggcgccg tcaccaataa
      541 cgtcatatga tcgaactccg gcccaaactc gccctggttg ttcgcgactc gtgcagaaag
      601 tcgtacaacg ccaaatccca gtgtgcgtaa cagactcgca aaaagaccgt ttagctcata
      661 acagaacccg ccgcggcggc gcacgacaat cttctcgaac agtgcctgat cttcgagaac
      721 gatgggctcg cccatatgaa tacttagatt ctcgaaggga actgtttgca gatgcgccac
      781 gtgcaactgg cgcagagtct cagcggtggg cgtggtgggc ccagaatagt tgatgcgttt
      841 caggtagcgg gtggtttggc tcataacagt ccgcgaagcg gacgactgat agtagcccaa
      901 cacttaaagt gcttgttagc catcaaagac ttgatccaat tctttgatcg acgccgcccc
      961 ggcaaagcct tcaaacgaac cttccgccag ctgcttcgcc gcgcgtatga atcctcccca
     1021 cgcaacgcga gctaatgcag agcctactga gatacgacgt actcccagat cagccagccg
     1081 cgtgactgtc atggtgggat tcggtcgtga catcaagacg ttcacgggct tcggggcaac
     1141 cacttttacg atggccttga tttgctctgg ttcagcgaga cccggcgcat aaagacaatc
     1201 cgcgccggcg tcggcaaaag cagtgaggcg ctcaagcgca accttcaacg gagttgcatg
     1261 tccgaccaaa gccgcttcac aacgagccgt caggaccaca gggataccgg acttatcgat
     1321 cgcttctcgg gccgccttaa ttctttcgag tgccaggctg gtttcataaa gcggcgagtt
     1381 agcgcgtccc gtgttgtctt cgattgataa gccagccact ccagtttcaa tacacaacga
     1441 aacactagct gctacctctg cgggtgagtc agcgtacccg ttctgaaaat cggcgttcac
     1501 gggtaacggt gtcgccgctg cgatctcggc gacgtgccct aacatttcgc cacgcttgat
     1561 cgccagtact gaatcgggaa gtccgagggt gaaagcataa ccggccgaag tagttgccag
     1621 tgccttaaag ccaagatgct gcaagtaaac cgcggtgccg atgtcccagg ggttcgggag
     1681 gacaaaacat ccggattcgt gcagcgcatg aaaagtggca acggcgctta tttgtttgtt
     1741 ggtcatgtcg agaatcgttt cagtgtcgag atgggagcat aatccccccg ctgagcagcc
     1801 atcagtatct ccaactcttc agatcagcgc ccggaggtgc gctcttcaga atgcgttcga
     1861 caattttagg agcgtaaaac tggtggtagc gaagtcgcga cgttgcattc ggccgcgtgt
     1921 gatcgccgtt ccaacaatgt tccgcgcgcg gctcataatc gacttcgccg ttgtagtgag
     1981 ggttcttcgt agacttgaga aaatcttcga cgagatagac tgcgttgtta agaaagtaat
     2041 tgtcagcctc gccaacatag atgtgaagtt tgccttcgag ctttggtccc agcttcgccc
     2101 agtcgcgctt gagaatgtga ccaaggtcgt agtgctcacg ccaataagcc gcgacggaat
     2161 gatcgatctc tcccgtaagt ttgttccaga ttggtttagg ataaccatcg ggtcctactg
     2221 gtgagtacac cgcctcccaa atatcaaatt gacctcctga tctgctcttc gtgccgaggg
     2281 cgagttccat gtggttctcg tcttcggtgg tggctgcgag ttccccgaga tagtttcgtt
     2341 ttcctgggcg cgggaccttt ttccatgggc tatccgcgta gtaggcattt ttgtgttcgt
     2401 agatattgac aaccgtatag gcgcgaaaat caattggatc gggacacgcg ccccacgcac
     2461 cgttgtattc gtcgggatag aatatctgcg ccgcaagcgc ctcccagcct ccggttgagc
     2521 cgccatacat gaaccgcgcc cacccttgtc caatgccgcg aaactgtttc tcaatgtacg
     2581 gcaccaattc ataagtaatc gcgtcgccat aaggaccgag gttcgcggag ttcacagcgt
     2641 acgaatcgtc gtagtaagga tttgcatgct gaatctcgat gatgatcatg cgcgggaaat
     2701 tcgggcccgt ccattcctgg tagaacttat aggcgtgctc ttgcacgatg cgattatagc
     2761 cggcaagttt gaagcgctcg ctgtaatcag gcttgagatt gggatctggc ggtgtctcgc
     2821 ggaagccacc aaagtcagcg ggaaaatgac catgaaagat cacgagcgga taacgtgcat
     2881 tgggatgaga atcgaatcct tcgggcaaca aaatgttggc gccgaggtgc atgggccggc
     2941 cccaaaaatc cgagagcaac ttactttgaa ttctgacgtg cttgatgtac ttggtgtctt
     3001 gcggcggcgc gatcggcgga atctcctgat cgagcttaag cttgatggtt tccgtctttt
     3061 gcggatcgat agctaacttc tgcggcttgc tatagaggtt tcccggcgcg cgactccact
     3121 gttgtccctc gccgcgatcc attggaagct tgacggtatg tccgtctgaa cgcttgaagg
     3181 tttcgtagcg atgaagcagc gcttggatcc aatattcacc cggcggtagc tgtgccaggc
     3241 tgcgtaccgg atagccaaac gcgctcgcat caacaattgc ttcggcatct ggcttcaatc
     3301 cttcaacgtc ggtaccgaac acttgttgcg tgttcaggtc ctcgttgatc tgaaagcgcg
     3361 gctcgctctc attgtttttt gagatcagca ggagcaggcg cccatcaagc ggcgtgttgc
     3421 tgcgctcggt agcgaatgag atggcgaagc gcaggttagc ttgtgattca ttctctagtt
     3481 ttgctttctg gggaaatgcg agcgagatca gcaagaagag agagagagct acgaatgatc
     3541 gcgcatagtt tgaagtcatc tggggattct ccgtttttgt gaacaaaagc ttcagtcgtt
     3601 attcatgtta tttagtgtgg ttctgtggat cgtaaacctc gccaacaaat gaggacgctc
     3661 caccaaaaga cacgaacaga caccaaatct ccaatcggac tcccaaatct tgatcgcacc
     3721 agcgaatgac tgctcaatga taatccggat cggctttcat tctcaccggg ctttagcccg
     3781 gtgctgattg cgcgcacgaa gactggaaac cgtttaaacg gtttccgtca tctacccgag
     3841 tcgtcccccg ggctaaagcc cggtgagaac ggtcattgtg cgaagtcact aatcggttgc
     3901 gtggttgcat cttcgtcacg gccgatcatc agctgcggcg gcgcgccatc cgcgtattga
     3961 agtttgtaga gcatccaata gagaccatgc tgcgccagca actcctgatg cctaccctgt
     4021 tccctgatct cgccatggtg caacacgata atctgatcgg ctcgctggat tgttgagagc
     4081 ctgtgcgcaa ccacaattga agtgcgatcc tgcatcaccc ggccaattgc ttgctggatc
     4141 gattgctcgg tttcagtatc aatcgagctg gtagcctcat ccagaattaa taacgccgga
     4201 tcgaacgcga gtgcgcgcgc gaacgagatc aactgctttt gtccgaccga taatcccgcg
     4261 ccgcgctcct tcacttccga cttgtacccg tcccgcaaac gctcgatgaa tcggtgcgcg
     4321 tgtacttcct gcgccgccca atgcacacgc tcgtcggaaa tatcatcacg gcctaaacga
     4381 atattacttt caactgtgcc gctaaaaagg aatatgtcct gaagcacaac cgcaaaattg
     4441 cggcgcagcg attccagatc ccaatcccgc aggtccactc cgtccagcag aatctgcccg
     4501 cgctggatat cgtagaaacg catcagcagg ttcgtgatcg tggttttccc ggaacctgta
     4561 tgaccaacga gcgcgacgga ctcaccagga tttacagtga acgaaacgcc cttcagcacc
     4621 cagtcttcgt ccttgtaagc gaaccagacg tcgcggaatt caatcaagcc gcgcgcacgt
     4681 tcgttgcgta agggttccgc cggagaggtg atagcaatcg gcaggtcgag tgtcttgaag
     4741 atacgatgcg aggctaccac cgccgcctgc aacacgttgt acttgtccga aatgtccctg
     4801 attggctgaa acaggaatcc ggagtactga atgaacgcta ccagcgcacc aagcgttagc
     4861 gcggaattca tcacgcgata gccgccatac cagattacga gtgcgatgcc gaccgcgccg
     4921 ataaaatcga caagcgggaa aaagagtgcg tagtaaaaaa ttgtatcgag gttcgctttg
     4981 cgatagtcgt cattgatgtc gtcaaacttg cccagtgatt tactttccgc attgaaaatc
     5041 tgcacggtct gggcgcccgc gaagtgctcc tgcaagaagg cattgatgcg agcgatacgc
     5101 gtgcgcacca tgtcataacc gcgccgcgcg cctttacgaa accaggtggt cgccgcgaag
     5161 agcagcggca cggtcaacag ggtgacgagc gtcagccgca cgtccatcca caacatcaca
     5221 ccgatgattg caaagatcat cacgaggtcg ccgagcacgt ctgtcacgcc ctgcgtgaag
     5281 agttcattga gcgcatctac gtcgttagtg agccgcgtca tgatgcgccc caccgggttg
     5341 cgatcgtagt aggcaacttc ctgatgttgc agtttgtcgt atagttcgcg cctcatatcg
     5401 aacattacat actgaccaac agttttcagt agcaggtcct gtaagtaaga aaagatcagg
     5461 cgcaggaact gaacgccggc aaacaatccc acaagcaaca tcaaaccgtc ggcggaccgg
     5521 ggcaggatgt accagtcgat cgcgtactgc gtaaactttg gctgcaaaat ac
//
LOCUS       DASUEX010000100         2532 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_709113, whole genome shotgun sequence.
ACCESSION   DASUEX010000100 DASUEX010000000
VERSION     DASUEX010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_709113"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..840
                     /locus_tag="VFV61_01705"
     CDS             <1..840
                     /locus_tag="VFV61_01705"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX4899215.1"
                     /translation="AIGGLPQFDSFEAMIKQMDEAGVEKVFITQCKMWSYRNKWMYMD
                     TKLEDVLQYTETYPDRFVGLAGYNPFRIKESLREIELAVTKHGFKGVYVHIFGFDIPL
                     NDAKMYPLYAKCVELNVPVSMQVGHVLEAMPSEGGRPMNLDRIACDFPDLKMIGAHTG
                     WPWVDELISVCYKWENIWFGVDAWLPKYLSPQIIQFIGSRMGSDRCIWGTNGLPWKES
                     LLQFCNLGLKQDVQRKVLRDNAIELFNLEQTKEAIPASLPAIEKTSATGLARDDGQAN
                     AFV"
     gene            884..1660
                     /locus_tag="VFV61_01710"
     CDS             884..1660
                     /locus_tag="VFV61_01710"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEX4899216.1"
                     /translation="MVGLQDFSGSTVIVTGGARGIGGAVVQAFAEAGADVVIGDLRIN
                     EAADAAMQLSEIYSRRIVAVEIDVTKHEDAKRLAKEAASLSGKIDVLVNCAGWDHLEP
                     FIKTSPDLWQKVLSINFIGLANVCHAVLPYLVERKQGAIVNISSDTGRVGSFGEAIYA
                     SSKAAINAFSKTLAREHARDDIRVNVVSPGLCDTPLIEEMRQDQFTNKILGAIVNLIP
                     MKRLGRPEEVAALVLFLASDAASYITGQVFSVNGGLNMVG"
     gene            1700..>2532
                     /locus_tag="VFV61_01715"
     CDS             1700..>2532
                     /locus_tag="VFV61_01715"
                     /inference="COORDINATES: protein motif:HMM:NF017828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HEX4899217.1"
                     /translation="MTTGAINCEDIIYDLDFSSVREWKSAEPNRKAIGYFPVYAPVEI
                     IHACGMLPVGLNGAGDRLDIQYADARFGSFICSIIKTTLELGLTDHLAPFDGLLFSSI
                     CDSARNLAFVMKRNFPDRYIDFLHLPHNPNSPSSVEFLLSEYRRLIDALNELGGEQFS
                     DDALRESIALYNENRALTRQLYLERSERPHVLRTTELYPLIRAGNFLPVAEHSAMLRE
                     TLADLPTRTGKQRDSIRVVIEGSFCEQPPLDLIRILEEAGCYLVDDDLVLGPRWFVND
                     VP"
BASE COUNT          582 a          678 c          694 g          578 t
ORIGIN      
        1 gcgatcggcg gtttgcctca gtttgatagc ttcgaggcga tgatcaagca aatggatgaa
       61 gcgggcgttg aaaaagtctt catcacccag tgcaagatgt ggtcctatcg gaacaagtgg
      121 atgtacatgg acacgaaact cgaagacgtg ctccagtaca ctgaaactta tcctgatcgc
      181 ttcgttggtc tcgccggtta caaccccttc cgcattaagg agagtttgag agaaatcgag
      241 ctcgccgtta ctaaacacgg cttcaagggc gtctacgtac acatcttcgg cttcgacatt
      301 cccttaaacg acgccaagat gtatccgctt tacgccaagt gcgttgaact aaacgtcccg
      361 gtatcgatgc aggtgggcca tgtgctggaa gcaatgccca gcgagggtgg ccgcccaatg
      421 aatcttgacc ggatcgcctg tgactttccc gacttgaaaa tgattggcgc ccacacgggc
      481 tggccgtggg tggatgagct catctcggtc tgctacaagt gggaaaacat ctggtttggt
      541 gttgacgcct ggctgccaaa gtacctctcg ccacagatca ttcagtttat tggtagccgc
      601 atgggcagtg atcgctgcat ttggggcacc aacggtctgc cctggaaaga aagcctgttg
      661 cagttttgca atctgggact gaagcaggat gtacagcgaa aagtgctgcg tgacaacgcc
      721 atcgaactat tcaatctgga gcagacaaag gaagccattc cggcttctct acccgcaatc
      781 gaaaaaactt ccgcaactgg cctggcaaga gatgacggcc aagccaacgc attcgtatga
      841 ccgcagactc cattagtctg caaaaacatt ggagcgcaag aaaatggtag gacttcagga
      901 tttcagcggg tccaccgtca tcgtaaccgg tggagcacgc ggcatcggtg gtgcagttgt
      961 tcaggcattt gccgaagcgg gcgctgacgt cgtgatcggt gatttacgaa ttaatgaagc
     1021 cgcggacgct gccatgcaac tcagcgagat ctacagccgg cgaatcgtag cggtcgagat
     1081 cgacgtcacc aaacatgaag acgccaagcg tctggcgaaa gaggccgcca gcctgtccgg
     1141 caagattgat gtcctggtta attgtgccgg gtgggaccac ctggagccct tcataaagac
     1201 atcgcctgac ctatggcaga aagtgctttc gatcaacttc atcggcctcg caaatgtctg
     1261 tcacgccgtg ttgccgtacc tagttgagcg aaagcagggt gcgatcgtca atatcagttc
     1321 tgacaccggc cgggttggct cttttggcga ggcgatatat gccagctcaa aggccgcgat
     1381 taacgcgttt tcaaagacac tagcgcgcga gcacgcgcgc gatgatatcc gggtgaatgt
     1441 tgtttcgccg ggcttatgcg acacaccctt gatcgaagag atgcggcagg atcaattcac
     1501 caacaagatt ctgggtgcaa tcgtgaacct gattccgatg aagcgtctgg gacgccccga
     1561 agaggtggcg gccctggttc tgttcctggc ctctgacgcg gccagctaca tcaccggaca
     1621 ggtgtttagc gttaacggtg gactcaacat ggtgggttag tccgaaagga tccggctcaa
     1681 aagaccttta gggggacaca tgacgaccgg cgctatcaac tgcgaagata tcatctacga
     1741 tcttgatttc agctcggtgc gagagtggaa atccgccgaa cccaatcgca aagcgatcgg
     1801 ctacttcccg gtctacgccc ccgtcgagat cattcacgct tgcggcatgc tccctgtcgg
     1861 tttgaacggt gccggagacc gtctcgacat tcagtatgcc gatgcgcgtt ttggttcctt
     1921 catttgttca attatcaaaa ccacacttga gctgggacta acggatcatc tggcaccgtt
     1981 tgatgggctg ctcttttctt caatctgcga ctcagcacgg aaccttgcgt ttgtcatgaa
     2041 gcgcaacttt ccggatcgct acatcgactt tctgcacctg ccgcacaatc ccaattcacc
     2101 ttcgagtgtt gagtttctgc tgagcgagta tcgacggctg attgatgcgt tgaacgagct
     2161 cggcggcgag caattcagtg acgatgcgct cagagagtcc atcgccctct acaacgaaaa
     2221 ccgggctctc acccggcagc tttacctgga acgtagtgaa agaccgcacg tcctccgcac
     2281 aaccgaactt tatcccttga ttcgagcggg aaatttcctc cctgtggcgg aacactcggc
     2341 catgcttcgc gaaaccctgg cagatttgcc aactcgcacc ggcaagcaac gagactccat
     2401 tcgcgttgtg attgaaggat ctttttgcga gcagccgccg ttggacctga ttcgcatttt
     2461 ggaagaggcc ggctgttacc tggttgatga cgacctcgtg ctggggccgc gttggtttgt
     2521 gaacgacgta cc
//
LOCUS       DASUEX010000101         4793 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_21976, whole genome shotgun sequence.
ACCESSION   DASUEX010000101 DASUEX010000000
VERSION     DASUEX010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4793
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21976"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="VFV61_01720"
     CDS             <1..507
                     /locus_tag="VFV61_01720"
                     /inference="COORDINATES: protein motif:HMM:NF013654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HEX4899218.1"
                     /translation="ADSTAEVLKSWEAIKAGEKRAAQTTGDNQQTSLLDGVSAKVPAL
                     MEAHQLSTKAARVGFDWEKLQDIFDKLHEEIAELRAAIQTHVSSKDEENHARVREEIG
                     DLLFAVTNIARHLHVEPEAALKLTNRKFRQRFGHIEKSLRSEQRSFESTTLAELEAHW
                     QEAKGSRK"
     gene            complement(648..2063)
                     /locus_tag="VFV61_01725"
     CDS             complement(648..2063)
                     /locus_tag="VFV61_01725"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEX4899219.1"
                     /translation="MIKRNYQRSQGVIAGLAMLAIFLMPISVFTQTKISYHSNKYSVQ
                     DDVKLGRQAAAEAEQQFPLLRDNEVESYVENVGQRLVNNIPAEFQHREFQYFFKVVNA
                     RDINAFALPGGPMYVNRGMIESAGREGEMAGVMAHEIAHVALRHGTAQATKGQKYGLL
                     AGILGIGGQILGGPAGAAAQIAGQGVGVYFLKFSREYETEADILGAQILARAGYDPRD
                     LATMFQRIQQQGGGSSGGFLSSHPSPQNRYARINQEAQYLRVENPIRDSREFDRVKAK
                     LRGYGTAPTMAEIARSGQRYPSEGGTTSNAPGGRVDYPSTRYRNYSEFGFLSVSIPDN
                     WRELRGSNSVWFAPEGGYGQYQGQDVFTHGVNFGVFRSQSRDLRQGTQELVNSFTQGN
                     GNMRQSGGTARSNIGGRSGYNVQLTNLNEATGQRENVTLITTQLRNGDVFYMIAVAPQ
                     SEARSFTNAFNTILRSTRLSD"
     gene            complement(2328..3014)
                     /locus_tag="VFV61_01730"
     CDS             complement(2328..3014)
                     /locus_tag="VFV61_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899220.1"
                     /translation="METKKTKYDTNPLDPDVANRTEEVWGEEEGGPGTRQIKGATRDV
                     GYSANESARSNVYSEAPTRTFDNQPLDAPYHSVFAPPVQQQPPSIYQPNPYLQTPIST
                     RPTSRSVAGVGLPEKWAMMLPYAPFYIGVVASLVELFLVPRKEVKVRFHAAQGLALHL
                     AILVLQTLFGVLGSITDSSFGGSLFKLAATVFLIISMVRVWKGEPHRIAPIAEPAQWF
                     NERIEPRNKD"
     gene            complement(3058..3474)
                     /locus_tag="VFV61_01735"
     CDS             complement(3058..3474)
                     /locus_tag="VFV61_01735"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HEX4899221.1"
                     /translation="MLTLRTSHFVNIVAHAREADPAECCGLIGGSESFLAETIYQLRN
                     VAPNNHVSYEVAPEELFAAQREMRQRGEQLVAIYHSHPRAAEPEPSETDVRLAYYPSA
                     TYLIVGLGGREPVMRAFRISEAERRWERVEYAVADA"
     gene            complement(3685..>4793)
                     /gene="aspS"
                     /locus_tag="VFV61_01740"
     CDS             complement(3685..>4793)
                     /gene="aspS"
                     /locus_tag="VFV61_01740"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEX4899222.1"
                     /translation="RADRQPEFTQLDVEMSFATPDQIYSLIEGLFAGVFKLIGVELPL
                     PFPRLTYAEVIRRFGSDKPDLRIPGMELQDLSGALQHTTFAPYAAALEGGGQIKGIAV
                     AGGAALSRKVLDDLQEFAKRYGAAALAWIKMGDEVSSSLLKVLGADVINELATLAGAQ
                     KGDAVLIVAGKSKTVAASLGALRNEVARREKLIPEGAYAPLWVTDFPMFEYHEEDGRY
                     YSMHHPFTSPVDEDLTLLERAVDGGEPELLGQIRTKAYDPVINGVELASGSIRIHRQD
                     VQRLVFKALGMTQEEARARFGFFLDALEYGTPPHGGIAPGIDRLMMVLAGTDNMRDVI
                     AFPKTASAADLMIDAPGEVDPKQLEELRLKVVKD"
BASE COUNT         1094 a         1384 c         1280 g         1035 t
ORIGIN      
        1 gccgatagca ccgctgaggt tctaaaaagt tgggaagcaa tcaaagccgg ggagaaacgg
       61 gccgcgcaaa ccactggaga caaccagcaa acttcattgc ttgacggtgt ttcggcgaag
      121 gtgccggccc tgatggaagc ccatcaactg tcaacgaaag cagcgcgagt cgggttcgat
      181 tgggaaaaac tgcaagacat ctttgacaaa ctacacgagg agattgccga gctgcgcgct
      241 gcaatccaaa ctcacgttag ttcgaaagac gaagagaatc acgcgcgcgt gcgcgaggaa
      301 attggcgatc tgcttttcgc ggttaccaac atcgctcgcc atctgcacgt ggagccggaa
      361 gcggcgctga agttaaccaa ccgcaaattc cggcaacgct tcggacatat tgaaaaaagt
      421 ttgcggtcag aacaacgaag cttcgaatcg acaacactcg cggagctgga agctcattgg
      481 caggaagcga agggaagtag gaagtaggag gtaggcagta ggctgcgcca gcaggcatcg
      541 cagaaatgca ggctctaagc tctaagctca aagctcaaag ttcaaagacc caaacggcaa
      601 aaggcccgct tccgcgggcc ttcgtgttgg ggtgagtgga aatctactta gtcactcaac
      661 cgagtcgagc gcagaatggt gttaaaggcg ttcgtaaagc tccgggcttc gctttgcggc
      721 gccacggcga tcatgtagaa cacatccccg ttgcggagct gtgtagtaat cagcgtcaca
      781 ttctctcgct gtcccgtagc ctcattgagg ttggtcagct gcacgttata cccactgcga
      841 ccgccgatgt tggatcgcgc cgtcccacca ctctggcgca tgttcccatt tccctgcgtg
      901 aaactgttga cgagttcctg agtcccttgc ctgagatccc gactctgcga acggaagacg
      961 ccaaagttca caccgtgcgt gaaaacatcc tgaccttgat attgtccgta gccaccctct
     1021 ggtgcaaacc aaacggaatt actgccgcgt agctcgcgcc agttgtcagg aatgctgacg
     1081 ctaagaaagc cgaactcgct gtagttgcgg tagcgcgtcg acggataatc aacccgcccg
     1141 ccgggtgcat tgcttgttgt gccaccctcg gacggataac gctgtccgct gcgagcaatc
     1201 tccgccatcg tcggcgccgt tccataacca cgcagcttag ccttgacgcg gtcgaattcg
     1261 cgcgaatcgc gaatcggatt ctctacacgc aggtattgcg cttcctggtt gatacgggcg
     1321 taacgattct gcggactggg gtggctgctc aagaaaccgc cgctgctgcc gccgccctgt
     1381 tgttgaatcc gctggaacat ggtcgccaga tcgcgcggat cgtaaccggc gcgagccagg
     1441 atttgtgccc ccaaaatgtc ggcttcggtt tcgtactcgc ggctgaactt cagaaagtag
     1501 acgccaacgc cctgaccagc aatctgcgcc gccgcgcccg ctggcccacc aaggatctgt
     1561 cccccgatgc cgagaatgcc ggcgagcaag ccatactttt ggcctttggt agcctgcgca
     1621 gttccgtgtc gcagcgcgac gtgggcaatc tcatgcgcca tcacaccagc catttcacct
     1681 tcacgaccgg cggactcaat catgccgcgg ttgacataca tcggtccccc gggcaaagca
     1741 aaagcgttga tgtcgcgcgc gttgactacc ttgaagaaat actgaaactc gcgatgctga
     1801 aactctgcgg gaatattatt aactaaacgc tggccgacgt tctcaacgta actctcgacc
     1861 tcgttgtcgc gcagcaaggg aaactgttgt tcagcttccg ccgcagcctg gcgtcccagt
     1921 ttgacgtcgt cctgcacgct atacttgttg ctgtgatagc tgattttggt ctgcgtaaaa
     1981 accgagatcg gcatcaggaa aatcgccaac attgcgaggc cggcaatcac tccctgactt
     2041 ctttggtaat tccttttaat catttgctcc ctccgtttcg gaacctatca gtaactcagc
     2101 ggactgcgag agagtcattt tggaccgcga gctggggttc ttcgatgctt tcattaaact
     2161 ctcaaactac tcttcaaact ctcaaactac tcttcagatt ttggcgcagt gcaccaaatc
     2221 gcaacataga tatacgcttc aaggacgcca atcccagtaa aaacgctatg aaaatgggat
     2281 tcgcgtcgtc cagcttaata gatagcaatg aggatgccag tgaggagtta atctttgttg
     2341 cggggctcaa tgcgctcatt gaaccactga gcgggctcag caattggggc aatgcgatga
     2401 ggttcgcctt tccacacgcg aaccatagat atgatcagaa acactgttgc cgccagctta
     2461 aaaagcgaac ctccgaagga gctgtcggta atcgaaccaa gcacgccaaa gagtgtttgc
     2521 aggaccagaa tcgccaggtg caaggccaag ccctgcgctg catgaaagcg aacctttacc
     2581 tctttgcgag ggacaagaaa caactccacc aacgacgcga ccacgccgat ataaaacggc
     2641 gcatatggca gcatcattgc ccatttttcc ggtaagccta ctcccgcaac gctgcgcgat
     2701 gtagggcgcg tgctgatcgg tgtttgcagg taaggatttg gttgatagat cgacggcggt
     2761 tgttgttgca caggcggggc gaataccgaa tggtaaggcg cgtcgagcgg ctgattgtcg
     2821 aaggtgcgag tcggcgcctc tgaatagacg ttgctccgcg cgctttcatt agcagagtag
     2881 ccgacgtcgc gcgttgcgcc ttttatctgg cgcgtgcccg gtccaccctc ctcctcgccc
     2941 caaacctctt cagtgcggtt tgctacgtca ggatccagtg gattggtgtc gtactttgtt
     3001 ttctttgtct ccatcgccgg agtctctacg caaataacgc tgtgacggtt cctaatctta
     3061 cgcgtccgct actgcatact caacccgctc ccaacgccgt tccgcttcag agatgcgaaa
     3121 cgcgcgcatt accggctcgc gcccgccgag cccgacgatt aggtaagttg ccgaagggta
     3181 ataggcgagg cgcacgtcag tttcggaagg ttccggctcc gcagcgcgcg gatgagaatg
     3241 gtagatggca accagttgct ctccccgttg acgcatctct cgctgcgccg cgaagagttc
     3301 ttcgggggcg acttcgtaag agacgtggtt gttaggcgcg acgttgcgga gttggtagat
     3361 agtttcggcg agaaagcttt cggatccacc gatcaatccg cagcattcag ccggatcggc
     3421 ttcacgggca tgtgcaacga tgttgacgaa atgagaagta cgtagagtga gcaagatatt
     3481 gtggataacg gcaacgcgta gcgttgttgt gattttagcc cggcatttca atgccggggc
     3541 gcgacggacc gatctagcgc gtcgcggcag cgacgcctga attaattcgc cacgctttca
     3601 atcgtcgcta ccgggctaaa atcatcacgc cgctacgcgt cgtttcgccg ttcccatttg
     3661 agaggatccg accacgtggc tgattcagtc cttcacaact ttcaagcgca gctcttccaa
     3721 ctgtttcgga tctacttcac ccggcgcatc aatcatcaga tccgctgccg aggccgtttt
     3781 gggaaaagcg attacgtcgc gcatgttgtc agtgccggct aaaaccatca tcaaacgatc
     3841 gattcccggc gcgatcccac catgaggcgg cgtcccgtac tcaagcgcat ccaaaaagaa
     3901 gccaaagcgc gcccgcgctt cttcctgagt catgcccagg gccttgaaga ccagccgctg
     3961 cacatcctgc cggtgaattc gaatcgagcc actcgctaac tccacaccgt taatgacggg
     4021 atcgtaagcc ttcgttcgta tctggcccag caattccggt tccccgccgt caacggcacg
     4081 ttcgagcagg gtcagatctt catcaaccgg ggaggtgaac ggatgatgca tcgaatagta
     4141 acggccatct tcctcgtgat actcgaacat gggaaagtct gttacccaga gcggcgcgta
     4201 ggcgccctca ggaattagct tctcccggcg cgccacttcg ttccgcaaag cccccaggct
     4261 cgccgccacg gtcttactct ttcccgccac aatcaaaacc gcatcaccct tttgtgctcc
     4321 agctagcgtt gctaattcat tgatcacgtc ggcgcccagg accttgagta gcgatgagga
     4381 aacctcatca cccattttta tccaggccaa tgccgcggca ccgtagcgct tggcaaactc
     4441 ctggaggtca tcaaggacct tccgcgacaa ggcggcaccg ccagccactg cgattccctt
     4501 tatctgtccg ccaccttcta aagcggcggc ataaggcgca aatgttgtgt gctgcaaagc
     4561 tcccgacaga tcctgcaact ccattccggg aattcgcaga tcgggcttgt ctgatccaaa
     4621 gcgccgaatt acttctgcgt aggtcaaacg aggaaacggg aggggcaact caacgccaat
     4681 caatttgaag acgcctgcga agaggccttc aatcaaactg tagatttgat caggcgtcgc
     4741 gaagctcatc tcgacgtcaa gctgcgtaaa ctcaggctga cggtcggcgc gca
//
LOCUS       DASUEX010000102         2850 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_709256, whole genome shotgun sequence.
ACCESSION   DASUEX010000102 DASUEX010000000
VERSION     DASUEX010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2850
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_709256"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="VFV61_01745"
     CDS             complement(<1..272)
                     /locus_tag="VFV61_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899223.1"
                     /translation="MAAEKQVTGKRMIFWTPARVAATVMVLTLVAMFGASSCNSTDER
                     VTREPAPKVNAAPVNPAAPAPAPASAMATLPATVLDAELKAVNGAP"
     gene            complement(360..1514)
                     /locus_tag="VFV61_01750"
     CDS             complement(360..1514)
                     /locus_tag="VFV61_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899224.1"
                     /translation="MKRCPKCNRSFPDESQKFCTVDGGLLIAEPTFDPSATMRATSAE
                     LGLPTETLSSQAATSRKLPDMAETILSTGSSATGATSNPLPPAESVATEAPTSSPLPP
                     LPQSTVTAETGSLPPERKKSKLPLVLGILAVLLLLGIGGLAAAFFFVVKPRLDQMQER
                     PIVVRETPSPEVPATTNEQVATNPEPTPTPDAFEPPANTTKFENSNANLDGKLAEHYL
                     DFSFYYPEGWQTDPKAGVAGATNFVKVERRLPPDFTQENFAVGWYTSQGTFEADTPLF
                     PRLVELLSANLSKDFPGYRKVSEGPTKINSLDAYEFRFVSLSKETEKGDIQVWGRVVF
                     LPSGTAGQTTGATLIMLATSLAPELSGVEDVGGKGEMPVILESFRFAKKS"
     gene            complement(1549..2808)
                     /locus_tag="VFV61_01755"
     CDS             complement(1549..2808)
                     /locus_tag="VFV61_01755"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX4899225.1"
                     /translation="MKELAMVRSVLVTTALGLGLMLLSAPGAFGQAVGADVGGGAGIF
                     RPKNPETRKRTAKPATPVTKPGTTSRATPRGGRVTPPANDDRVEDLLDKGNELRDARR
                     FAEAEEAYQGVLRLRPRDARAAYGLGNVFTDQQRWDDAETSYRNAVQWNPKSVDALVA
                     LSVVLVQPSTGAGNAKRLADAESFARRAVQIEPMNAVGWDRLGVAMETRGSFTPELEH
                     SYRRAIEVDPEFAVGYAHLARVLKRFGKQVEAAPLYDKAFELAKDPATLNLIAGSMQE
                     EQLWEKSGPVIRRALELDARNPTSLLLMGRMLLVLKRYDEAEPYLKTATEVSPRAFQP
                     FNLLGRSYLALGRLADAEAAYERAAEFAPAGDRKQLAGAFGFAGIGDEYMKAKENASA
                     ERAYQRALDLDPGNQELQQKLSKARSR"
BASE COUNT          596 a          846 c          790 g          618 t
ORIGIN      
        1 atgggcgcgc cgtttacggc tttcagctcg gcatccagca ctgttgccgg cagagtggcc
       61 attgcgcttg ccggagcggg agcaggggcg gcagggttca cgggtgcggc gttaaccttc
      121 ggcgctggtt ctctggtgac gcgctcatcg gtggaattgc aactggaggc accgaacatc
      181 gccaccagtg tcagcaccat aacggttgcc gccacccgcg ccggtgtcca aaaaatcatc
      241 cgcttaccgg ttacctgttt ttcagcagcc atagaataat ttcaacaccc aactaaaact
      301 taacatcgct tcgagaataa catccgcccg caaggtcgtc gaattagcga gcactcacgt
      361 caggacttct tcgcaaacct gaacgattcc aggatcaccg gcatctcacc tttgccaccg
      421 acgtcttcaa cgccactcaa ctcgggagca agagaggttg cgagcataat caacgttgct
      481 ccggttgttt gacccgcggt gccactggga aggaaaacca ctctacccca gacctggatg
      541 tcgcctttct cggtctcctt cgagagactc acaaaacgga attcgtacgc atccagcgaa
      601 ttgattttcg ttggaccctc cgaaaccttt cggtacccag gaaaatcttt ggagagattc
      661 gcgctcaaga gttcaaccag acggggaaac agcggagtgt ctgcctcgaa ggttccctgg
      721 ctggtgtacc aacctacagc aaagttttcc tgcgtaaagt cgggcggcaa acgtctttcc
      781 actttgacga aattcgttgc gcccgcaacc ccggccttag gatctgtttg ccagccttcc
      841 gggtaataga aggagaaatc gagatagtgt tcagccagct tgccatccag atttgcgttc
      901 gagttttcga acttcgtcgt gttagccggc ggctcaaaag cgtctggcgt gggagttggc
      961 tcgggattcg tggccacttg ctcgttcgtg gtggctggca cttccggtga aggagtttcg
     1021 cggaccacta ttggacgctc ctgcatctga tcgagacgcg gcttgaccac aaaaaagaac
     1081 gcggccgcaa gtccgcctat tcccagcagg agaagcaccg caagaatacc gagcactaag
     1141 ggcagcttcg atttcttcct ttccggcggc aggcttcccg tttcggccgt caccgttgac
     1201 tgcggcaggg gcggtagcgg cgaagacgtg ggtgcctcgg tggctacgct ctcagcggga
     1261 ggaagggggt ttgaagttgc tccagtcgcg gagcttccag tagacaggat tgtctcggcc
     1321 atgtccggca gcttccgact ggtggcggcc tggcttgaaa gagtttcagt gggcaatccg
     1381 agttcagcgg aagttgcccg catggtggcg ctcggatcaa aagtcggctc cgcaatcagc
     1441 aggccgccat caacggtaca aaacttctgg ctctcgtccg ggaaagaacg attacattta
     1501 gggcaacgct tcatttgatg aagtcctctc ttatgatctc aactgaaatc aacgcgaacg
     1561 ggccttagaa agtttctgct gtaactcctg attgccagga tcaagatcca aagcacgctg
     1621 gtacgctcgc tcggcgctgg cgttctcttt ggctttcata tactcgtcgc caatgcccgc
     1681 gaacccaaac gcgcccgcga gctgtttgcg atcaccggcg ggagcaaatt ctgcggcgcg
     1741 ttcgtaagcg gcctcggcgt cagcaagacg accgagggcc aaatatgatc gtcccaggag
     1801 gttgaagggc tggaaagcgc ggggactgac ttcggttgcg gtcttgaggt acggctcggc
     1861 ttcatcgtac cgcttaagga ccaacaacat gcgccccatc agtaatagcg acgtgggatt
     1921 tcgggcgtcg agttccagcg ctcgcctgat caccggtccg gatttctccc agagttgctc
     1981 ttcctgcata gacccggcga tcaggttcag tgttgccgga tccttggcaa gttcaaaagc
     2041 tttgtcataa agcggagcgg cctcaacttg cttcccgaag cgctttaaaa cacgcgccag
     2101 gtgtgcataa ccgaccgcaa actccggatc aacttcgata gcgcgccggt acgaatgctc
     2161 cagttcagga gtgaatgaac cacgggtttc catcgcgacg cccagtcgat cccaacccac
     2221 cgcgttcatt ggctcgattt ggacagcgcg acgagcgaag ctttcagcat cggccagccg
     2281 cttggcgttg ccggccccgg tacttggttg caccaacacc acactcaggg cgaccagggc
     2341 atcgacactt tttggattcc attgcacagc attgcgataa gacgtttccg catcatccca
     2401 acgctgctga tcggtgaaga cattacccag accatacgca gcgcgcgcgt cgcgcggccg
     2461 gagtcttaag accccctggt aagcttcctc cgcctcagca aaacgtcgcg catcccgtaa
     2521 ctcatttccc ttatccagca gatcctcaac gcgatcgtcg ttggcgggag gggtcacgcg
     2581 cccaccgcgc ggcgtggctc gggaagtcgt gcccggtttt gttaccggtg tcgccggctt
     2641 agcggttcgt ttgcgggtct cgggattctt gggtcgaaag attcccgcgc cgccgccgac
     2701 gtccgccccc acggcctggc caaaagcgcc cggtgcgctg agcagcatta accctaaccc
     2761 caacgccgtc gtcaccaaaa ccgagcgcac catcgctaat tctttcattg cctttctcct
     2821 gaacctgttg gaagtgaaag tcgagattgt
//
LOCUS       DASUEX010000103         3405 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1614749, whole genome shotgun sequence.
ACCESSION   DASUEX010000103 DASUEX010000000
VERSION     DASUEX010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3405
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1614749"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..381)
                     /locus_tag="VFV61_01760"
     CDS             complement(<1..381)
                     /locus_tag="VFV61_01760"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX4899226.1"
                     /translation="MNRTDVLIIGCGIAGATTALRLARNSERQITIVTRAPDPHESNT
                     RYAQGGIVGRGIDDDAELLEADILEAGAGVSLPAAARILATEGPSLLHEVLEKTARVN
                     FDQDSNGHALFTNEAAHSRRRILHV"
     gene            complement(402..1448)
                     /gene="nadA"
                     /locus_tag="VFV61_01765"
     CDS             complement(402..1448)
                     /gene="nadA"
                     /locus_tag="VFV61_01765"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: protein motif:HMM:NF006879.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEX4899227.1"
                     /translation="MIKETDPEKMYRAMQAKLSNVVPDFELKLKSEIAAEINRLKAEK
                     NAVILGHNYMEPTLFNSVPDFVGDSLELARRAAQTDKDIIVFCGVRFMAETAKILNPT
                     KTVLLPAKKAGCSLAASITAEDVRELKRRFPGVPVVTYVNTYADVKAESDICCTSGNA
                     IAVVESLHAERVIFLPDEYLARNVAKETGKEIIFPTTDPRLKADDELNFQMIGWLGRC
                     EVHDKFTVADIANVRKQFPDVTILAHPECSPAVVAASDFSGSTGAMIRFVETSSAPHY
                     LLLTECAMGDNIAAANPEKDMLRLCSVRCPHMNEITLEETLESLQKNQYVIELAPDIL
                     ERAAQAVQRMIAIG"
     gene            complement(1470..2348)
                     /gene="nadC"
                     /locus_tag="VFV61_01770"
     CDS             complement(1470..2348)
                     /gene="nadC"
                     /locus_tag="VFV61_01770"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEX4899228.1"
                     /translation="MATEVKSEIKPDLDQALTADIESSIQRALSEDLGDGDATTNSIV
                     SPDARLEGLIKAKQQGVVAGLKVAAAVFNALDQKIEFQASVQDGAFVDNGVVVAAISG
                     PARSILTAERTALNFLGRMSGIATLTRQFVERVAGTAAKILDTRKTAPGLRAIDKLAV
                     RCGGGENHRFGLYDMILIKDNHIDHAGSLRRAVELARVARGELEIEVEARTLEHVKDA
                     IDLGVKRILLDNMSLEMMTEAVRLNRGRARLEASGNVSLATVRQIAETGVDFISVGEL
                     THSARVFDFSLIVKTT"
     gene            2575..>3405
                     /locus_tag="VFV61_01775"
     CDS             2575..>3405
                     /locus_tag="VFV61_01775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX4899229.1"
                     /translation="MVNANRSTTYLDDSGPGEAALADALISFCNSLPDPIFTFDSHGV
                     LLTANKSGALLQGGSATSLQGRRCCEMFWRVEGTENCIVERALETGQRVEVELLAGPE
                     EKPTLVIVQPETDAAITQRGAVVIARDISELRCAEAEALAQRSFMASIADRSPDEIYA
                     LDTNGRITWMNERGEAGSSLMLSGRYLIEFVAEESRELATDKLQRALAGEDAEFEMRA
                     VRVDGTIRDVEAHTSPLWKENEVTGTLVFLRDVTDRRRHRELQAQSDKLRAVGELAAG
                     V"
BASE COUNT          728 a          956 c          923 g          798 t
ORIGIN      
        1 aacatgaaga atgcgacggc gagagtgggc cgcttcgtta gtgaatagag cgtggccatt
       61 actatcctgg tcgaagttta cgcgcgctgt cttctccaga acctcatgaa ggagtgacgg
      121 accctcggtg gccagaattc gtgccgcagc gggcaagctt actcccgctc cggcttcgag
      181 aatgtcagcc tcaagcaatt ccgcgtcgtc atcgatgcca cgaccgacga tcccaccttg
      241 cgcgtatcgc gtatttgact cgtgaggatc gggcgcgcga gtaactatgg ttatctggcg
      301 ttcagaattg cgagcaagac ggagcgcagt cgtagcgccg gcaatgccac agccgatgat
      361 cagcacgtcg gttctgttca attggttctc tttgcgggta gctacccgat ggcgatcatt
      421 ctttggacgg cctgcgcggc tcgctccagg atgtccgggg cgagttcaat gacatattga
      481 ttcttctgga gtgactcaag cgtctcttca agtgttatct cgttcatgtg cgggcagcgg
      541 acgctgcaca gacgcagcat gtctttctcc gggttcgcgg ccgcgatgtt atcgcccatt
      601 gcgcattcgg tcagcaacaa gtagtgcggc gcggaactgg tttccacaaa tctgatcatc
      661 gccccggtgc tgccagaaaa atcagaagca gccacgacgg cagggctgca ttccggatgc
      721 gccaggatgg taacgtcggg aaattgtttc cggacgttcg caatgtctgc aacggtaaat
      781 ttgtcgtgca cttcgcaacg cccgagccaa ccaatcatct ggaagttcag ctcgtcatcg
      841 gctttcagcc gcggatccgt cgttgggaaa ataatttcct ttcccgtttc cttggcgacg
      901 ttgcgcgcca gatactcgtc cggcaggaag ataactctct cggcgtgcaa cgattcaact
      961 acagctatcg cattgccgga agtgcagcaa atgtctgact ctgccttaac atcggcgtag
     1021 gtattcacat aagtaaccac cggaacgccg gggaagcgcc gcttcaactc gcgcacatct
     1081 tccgcagtaa tgctggcggc aagagaacaa cccgccttct tggccggcag tagcacagtt
     1141 ttcgtaggat tcaggatctt ggcagtttcc gccatgaacc ggactccgca aaatacgatg
     1201 atgtctttat cagtttgcgc ggcgcgtctt gcgagttcca acgagtcgcc gacaaagtcg
     1261 ggaaccgagt tgaacaaggt cggctccatg taattgtgac caagaatcac tgcgtttttc
     1321 tcagccttga gacggttaat ctccgcagcg atttccgact tcaacttcaa ctcaaagtca
     1381 gggacaacgt tggacaattt cgcctgcatt gcccgataca ttttttccgg atctgtttct
     1441 ttaatcatct cttccagttt cgcttatctt caggtcgttt tcactattag actaaaatca
     1501 aaaaccctcg ctgaatgcgt caactctcct acggaaataa aatcgactcc ggtctcagcg
     1561 atctggcgca cagtcgccag acttacattg ccagacgcct ccaagcgcgc tcgcccgcga
     1621 ttcaaacgca cggcctcagt catcatttcc aggctcatgt tatccaataa gatgcgcttt
     1681 acgcccaaat cgatcgcatc tttaacgtgc tcgagggtgc gggcttcaac ctcgatttcg
     1741 agttcgcctc tggcgacgcg tgcgagttcg acggcgcgtc tcagactacc cgcgtgatcg
     1801 atatgattgt ccttaatcag gatcatgtcg tagagcccga agcgatgatt ctccccaccg
     1861 ccgcagcgca cggccagttt gtcaatcgct cgcaggcccg gagccgtttt acgcgtatca
     1921 agaatcttcg ctgcggttcc ggccacgcgc tctacgaact gacgtgtcag ggtggcgatg
     1981 cccgacatgc gcccgagaaa gttgagcgcg gttcgttcgg ctgtcaggat cgaacgtgca
     2041 ggtccagaga ttgctgcgac aacgacgccg ttatcaacga aagcgccatc ttgaaccgag
     2101 gcttggaatt ctatcttctg atccaaagcg ttaaagaccg cagcagccac ctttagtccg
     2161 gccaccacgc cctgctgctt cgctttgatc aaaccctcga ggcgcgcgtc gggtgagacg
     2221 atcgagttcg tagtcgcatc gccatccccc agatcttcac tcagagcgcg ctgaatcgag
     2281 ctttcaatat ccgcagtcag agcctgatca agatcgggtt tgatttctga cttcacttcg
     2341 gtagccatgc tgctgagttt acaacgtgac aatttccggt gctacccgct ggcgccaaca
     2401 ccttaaacgg caacattaac cggcgagtcg cgtgaccttt gcagcacaaa cagtaagctt
     2461 tttctcgttg cggaaaaact gctggcctcg tcccttgctc atttgctagg atgggtttgc
     2521 ctaccggggc cttcgagctc ccctcagcca accctgaaag tcatttgatt tccaatggtc
     2581 aacgcgaacc gcagcacaac ttatttggat gattctgggc ctggtgaggc tgcgcttgct
     2641 gatgccttga ttagtttttg caattcacta cctgatccaa tcttcacctt tgacagccac
     2701 ggcgtgttgc ttacagccaa caagagcggc gcgttgctgc aaggcggttc tgccacctcg
     2761 ttgcagggcc ggcgctgctg cgaaatgttc tggcgcgtgg agggtactga gaactgtatc
     2821 gtcgagcgcg cgctcgaaac aggtcaacgc gttgaagtcg aattgttggc agggccggaa
     2881 gaaaaaccta cgctcgtgat cgtgcagccg gaaacggatg ccgccatcac tcagcgcggc
     2941 gcggtcgtaa tcgcccgtga tatctcggag ttgcgctgtg ccgaggccga ggcgctggcg
     3001 caacgatcat ttatggcgag catcgccgat cgcagtcctg atgagatcta cgcgctcgat
     3061 acaaacggtc gcatcacctg gatgaacgaa cgaggtgaag cgggcagttc attaatgttg
     3121 tcgggtcgct atttgatcga atttgtggct gaagagtctc gggaattagc caccgataaa
     3181 ctgcaacgag ccttggccgg cgaggatgct gagttcgaga tgcgcgctgt gcgtgttgat
     3241 ggcacgattc gcgacgttga agcgcatacc tcacctttgt ggaaggagaa tgaggtaaca
     3301 ggcaccctgg tttttctgcg cgacgtcacc gatcgccggc ggcaccgaga gcttcaagca
     3361 cagtcagaca agctgcgagc ggttggcgag ctggcggctg gagtc
//
LOCUS       DASUEX010000104         6668 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_595179, whole genome shotgun sequence.
ACCESSION   DASUEX010000104 DASUEX010000000
VERSION     DASUEX010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6668
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_595179"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..700
                     /locus_tag="VFV61_01780"
     CDS             <1..700
                     /locus_tag="VFV61_01780"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX4899230.1"
                     /translation="HFPHEKVIREYQRALVLDPSLDEARNQLALVYCHIGAFEEALQE
                     SEKAIATNPNNNLAQFRIGETLNFQGKYEQALATLRGLPKDVNPALVGHQIALAQFNL
                     GRKTEAANTIEQFLKDYPEDNRGLFTSLEAVLAASNGQQRKAEDNIKLAVERGKGFGH
                     FHHTAYNIACAYAVMNKPDQAIKWLEVVAEDGFPCYPLFERDSNLNNLRQDTRFIAFL
                     SKQKEQWEHFRTIL"
     gene            complement(854..1564)
                     /locus_tag="VFV61_01785"
     CDS             complement(854..1564)
                     /locus_tag="VFV61_01785"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEX4899231.1"
                     /translation="MRVVKFFLVALLLVGLYVSSSQSIPSSRESSIALSGAHAAPLHD
                     GNDLTKTASAQRYLLDSSRSKFIARAFSGGLLWFKGHDHLVAVRDFTGEAQITPDSIM
                     PASLQITAKAASLVETRDVFTEPQKQIINRELREIVLLPEQYPDISFRSTNITGRAIG
                     NSVYDLKIVGALTLHGVTRQISIPTKVTLSGNELRAQGEFSINRSDFKVKATSALHGM
                     IRVRDKVKFEFDIVGNLR"
     gene            1862..3724
                     /locus_tag="VFV61_01790"
     CDS             1862..3724
                     /locus_tag="VFV61_01790"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX4899232.1"
                     /translation="MKTLLCKVLLVVYLVPSIALGQATDAKIKRVENGLLPAVLIKGD
                     PAWSLAERLKFYKVPGLSVAVIENYRVAWARAYGVKDLETKEPVTTETLFQAGSISKS
                     VNAVVAMKKVEEGKISLDENINNKLTTWKLPDNEFTAKKKVTLKNLLSHTAGTTVHGF
                     PGYAVNEKIPTLPDVLDGSGGANTAAVRVDFEPGTRFRYSGGGTTISQLAIMDIEKKP
                     YPDIARETVLAPLNMSNSTYSQPLPDDWRKKAATGYRANGSEVGGKIHVYPEMAAAGL
                     WTTPTDLAKFAIEIQLSIAGRSNKVLKKETAQLMTTAFMDQAGLGFFIERRGNAVYFG
                     HGGADEGFRAELLVNRDKGYGVAVMVNSDNGQILREVMRAVAQEYGWDEFLPKPYEIV
                     SLKPEQLAAAAGRFQVNPDRVLTIKAESDSAASASQSSRQQHGSSGAHPAITKLIVEP
                     TGDPAFDLLPISETTFVRRDANIKYELVRNAAGAVDTIRLITPDGSTNEGKRISADSL
                     VPAEFLLAGKIPEAIEGYRKIKREQPDNVSVEENRLNNIGYILMRQNKLPQALAIFKL
                     NVEFYPAAWNVYDSLAEAYMNNGDKELAIANYKKSLELNPANAGGAEMLKKLQQ"
     gene            complement(3866..4888)
                     /locus_tag="VFV61_01795"
     CDS             complement(3866..4888)
                     /locus_tag="VFV61_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899233.1"
                     /translation="MLSLFVFLLFSISAPAEPGSHLLQTNSSAQTPKLSATDRTRLVE
                     AFRLGDKLGDRIWPRWSKAPFAVLLVTPEYEFLIRHPLPSPDFTNLGYDRELASNVFY
                     RKRTMSPGLLATFPAISGSMVSTIVVGQAENTLAKTSTPWVITLLHEHFHQMQDSQPS
                     FYADVNALNLARGDQSGMWMLNYPFPYDRTEVQEQFAAMSELLAAAVRAPKRERALRV
                     RDYLAARRSFQKLVDPQDYKYLAFQFWKEGIARYTEYHLARLAAVNYRPTKEFRALPD
                     YRSFADVAHTTYDGIFRQLRTQKLGESKREVVYSFGAAEGLLLDQTKPDWRRRYFVEK
                     FDLGKL"
     gene            complement(4893..5225)
                     /locus_tag="VFV61_01800"
                     /pseudo
     CDS             complement(4893..5225)
                     /locus_tag="VFV61_01800"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
     gene            complement(5238..5699)
                     /locus_tag="VFV61_01805"
     CDS             complement(5238..5699)
                     /locus_tag="VFV61_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899234.1"
                     /translation="MNIRRFNWHIWAGFLLSFFGAVSYTLFFVDFPITRDFPWANLIL
                     FMIAAGLLFVGIRRAFAPDRSRLAKVGSSVLATLGVLIIGVFIFAILIAGRWLPPAQG
                     APRIGNKAPEFTLADASGKATSLAELLTTPINVTNTAKPRGVLLIFYRGYW"
     gene            5896..>6668
                     /locus_tag="VFV61_01810"
     CDS             5896..>6668
                     /locus_tag="VFV61_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899235.1"
                     /translation="MKTRSFCAILLLLSLSLMIACMQAPLTNQPNGDAPVSTAAEAAA
                     LKPALDSILANDIMQHTKVLSSDEYEGRGPGTKGEELSVKYITEQYQRLGLKPGNPDG
                     TYVQKVPLAGFTAQPTASFSAGGKKIDLEFPKDYVAVSRRFVPESKVENSDIVFVGYG
                     VVAPEYGWDDYKGVDVRGKTIVMLINDPAVPDTTDATKLDPKMFKGNAMTYYGRWDYK
                     VAEAVRRGAAGVLVVHETKAAGYPWDVPRNGATKPQFDLR"
BASE COUNT         1673 a         1767 c         1823 g         1405 t
ORIGIN      
        1 ccatttccct cacgagaagg taattcggga ataccaacgg gcactggtct tggacccgag
       61 tcttgatgaa gcgcgaaatc agcttgcgct cgtctattgt catatcggag catttgaaga
      121 agcgctccag gaatcggaaa aagcaattgc aactaacccc aacaacaacc tggcacagtt
      181 ccgcatcggc gagacgctaa atttccaggg caagtatgaa caggcgctcg ctactctgcg
      241 cgggctcccc aaggacgtca acccggcgtt ggtggggcac caaatcgctt tggctcagtt
      301 caacctggga agaaagacgg aggccgccaa caccattgag caattcttga aagattaccc
      361 ggaggacaat cgcggcttgt ttaccagttt ggaggccgta ctggcagctt cgaatgggca
      421 gcaacgaaaa gccgaagaca acatcaagct agctgtagaa agaggaaagg gatttggtca
      481 cttccaccat acggcttata acatcgcctg tgcttatgcc gtcatgaaca aacctgatca
      541 ggcaataaag tggttagaag tggtggctga ggacgggttc ccctgctatc cgctgttcga
      601 aagagattct aatttgaata accttcgcca ggacacccgc ttcatcgcct tcctgagcaa
      661 gcagaaagag cagtgggagc acttccgaac cattctgtga agcacggtta agggaaggcc
      721 gagcgagctg aaagagtctc cccataatca ggttactccc gtttgcttca gtattatttg
      781 cgcgcaaagt cctgagccgc tcgctcccgc cgatacgaga tcctaaggaa agcctttcga
      841 attcaacttc ccgctagcgc agattaccga cgatgtcgaa ctcgaacttc actttgtcgc
      901 gcacgcgaat cattccgtgt agtgccgaag ttgcctttac cttgaagtcg ctacggttga
      961 ttgagaattc accctgtgcc cggagttcat ttcccgagag cgtgaccttc gtcggaatgc
     1021 tgatttgccg agtcacgcca tgcaatgtta gcgccccaac aattttcaga tcgtacacgc
     1081 tgttgccgat ggcacggccg gtaatattgg tactcctgaa agagatgtcg gggtactgct
     1141 ccggcaaaag aacgatctcg cgcagctctc tattaatgat ctgcttttgt ggctcggtga
     1201 atacatctct ggtttcaacc agcgatgcgg ccttagcggt aatctgaaga gaagccggca
     1261 tgatcgagtc cggagttatt tgcgcttcgc cggtgaagtc ccgcacggct accaaatgat
     1321 catggccttt aaaccaaagc aaccctcctg agaatgcccg cgcgataaat ttgctgcgag
     1381 acgagtccag caaatatcgc tgagcagaag cggtctttgt aaggtcgttt ccatcgtgca
     1441 gcggtgcagc gtgtgctccg ctaagtgcaa ttgatgattc gcgacttgaa gggattgatt
     1501 gagaggaaga gacgtacaga ccgactagaa gcaaggcgac caggaaaaac ttcacgactc
     1561 gcacaaccac ctctgaatcc gagcgcggaa aacaattcgc tttcaaacgg cttcgaatga
     1621 tgctgcaaga aagggcgaat ccgggggaac caatctgggc acttagaata gactcccaaa
     1681 gttcaaagtc caaggtccaa gccctttttt tggaacggaa tcgtttaatt gtattgagcc
     1741 aacatcgggt aaatcgcgga ccctggacgt tgaaccttcg actttggact taacttctgc
     1801 ctggcaaccg tgataaaacg cagcctcccc agacatccac tcggcaccga ggatttccca
     1861 aatgaaaacg cttctctgca aagttctcct ggtcgtctac ctcgttccgt ccattgctct
     1921 cggtcaagcg acagacgcaa agatcaaacg cgtcgaaaac ggacttttac ccgcggtttt
     1981 aatcaaaggt gatccggcct ggtcgctggc ggagcgcctt aagttctaca aagtgccggg
     2041 attgagcgtt gcggtcatcg agaactaccg cgtggcctgg gctcgcgcct atggtgttaa
     2101 ggatttggaa acgaaggaac cggtgacgac cgaaacactg tttcaagccg gctccatcag
     2161 caagtcagtg aacgcggtgg tggcaatgaa gaaggtcgag gaaggcaaaa tttcactcga
     2221 cgaaaacatc aacaacaaac tgacgacatg gaaactgccg gacaatgagt ttacggcaaa
     2281 gaaaaaggtg acactcaaga acctcttgag tcatacggcg ggcacgacgg tacatggctt
     2341 tcctggttat gccgtcaacg aaaagattcc gactttgccg gacgtgctcg atggcagcgg
     2401 cggggcaaac accgcggcgg tgcgggtcga cttcgaaccg ggcacacggt tccgttactc
     2461 gggtgggggc accacaattt cgcagttggc aatcatggat atcgagaaaa aaccttatcc
     2521 cgatatcgcg cgggagacag ttctcgcgcc acttaacatg agcaacagta cttacagcca
     2581 accgctgccg gacgattggc ggaagaaagc ggccactggt tatcgcgcca acggcagtga
     2641 agttggcggc aagattcatg tttatccgga aatggcggca gcaggtctgt ggaccacgcc
     2701 gaccgacctg gcgaagtttg cgattgaaat acagttatcg attgcggggc gctcaaacaa
     2761 ggttctcaaa aaagaaactg cgcaattgat gaccactgcc ttcatggatc aggccggact
     2821 cggctttttc attgaaaggc gtgggaatgc ggtttacttc ggacacggcg gcgctgatga
     2881 aggttttcgc gccgagctcc tggtcaatcg cgataaagga tatggcgttg cggtaatggt
     2941 gaattcagac aacggacaaa ttcttcgcga ggtgatgcgc gcagtggcgc aggagtatgg
     3001 ctgggacgaa ttcttaccta agccctatga aatcgtttcc ctgaagccgg agcaactggc
     3061 agccgccgca ggccgatttc aggttaatcc tgatcgagta ttgactatca aggccgagtc
     3121 tgactccgcg gcaagcgcca gccaaagctc ccggcaacag catggtagca gcggcgccca
     3181 tcccgctatt acaaaactga tcgtcgagcc caccggcgac ccagcctttg atttacttcc
     3241 aatttcggaa accactttcg tgcgccgcga tgccaacatc aagtacgaat tggtgcgcaa
     3301 cgcagcggga gctgtcgata cgattcgcct gattacgcct gatggcagca ccaatgaagg
     3361 caaacgcatc tcggcagaca gcctcgtgcc cgcggagttt ttactcgccg gaaaaatacc
     3421 ggaggccatt gagggatata gaaaaatcaa acgagagcag ccggacaatg taagtgttga
     3481 agaaaaccgg ctaaacaaca ttggctacat actgatgcgg cagaataaac tgccgcaagc
     3541 actggcgatc ttcaaactca atgttgagtt ttatccggcg gcgtggaatg tctacgacag
     3601 cctcgctgaa gcctacatga acaatggcga caaagagctg gcgattgcga actataagaa
     3661 gtcgctcgag ctgaaccccg ccaatgctgg tggcgctgag atgctgaaga agctgcagca
     3721 atgaacgaaa gcggcattga gtgattgaat cattgaatca ttgagtcata gtcacgggcg
     3781 ccggaaatca gttattagtg tctgttagct tgcgaaactg ctcagcacag ctttccgagg
     3841 gattaagtaa tcggcggtct aaaatctaga gctttcctag atcgaacttt tcgacgaagt
     3901 agcggcggcg ccagtccggt ttagtctgat ccaacaacaa accctcggca gctccaaacg
     3961 aatacacaac ctcacgcttc gactctccca acttctgcgt gcgcaactgg cgaaagatgc
     4021 cgtcgtaagt cgtgtgggcc acatcggcaa atgagcgata gtctggtaag gcacgaaatt
     4081 cttttgtggg acggtagttc acagccgcca gccgcgcgag gtggtactcg gtataacgcg
     4141 caattccctc cttccaaaac tgaaacgcca ggtatttgta atcttgagga tcgacgagtt
     4201 tctggaaact gcggcgcgct gcaagataat cacgaacccg cagcgcgcgc tcgcgtttgg
     4261 gcgcgcgcac ggccgcggcc agcagttcgc tcattgcggc aaactgctcc tgtacttctg
     4321 tgcggtcgta aggaaatggg tagttgagca tccacatccc agactggtcg cctcgcgcca
     4381 ggttgagggc attcacgtca gcataaaaac tcggctgcga atcctgcatt tgatgaaagt
     4441 gttcgtgcag gagagtgata acccagggcg tagaagtctt agcgagggtg ttttctgcct
     4501 gacctaccac gatcgttgag accatgctgc cgctaatcgc tggaaaagtg gcgagtagcc
     4561 cgggagacat cgtgcgcttc cgatagaaga cattgctagc gagttcccgg tcgtaaccga
     4621 ggttcgtgaa gtctggagaa ggtagcggat gccgaatcag gaattcatat tccggagtca
     4681 ctaacagcac tgcgaacggc gccttactcc accgcggcca aattcgatcg ccgagcttgt
     4741 ccccgagacg aaacgcttcg accagtcgtg ttcgatctgt cgcgctgagt tttggcgttt
     4801 gcgcgctgct gttcgtttgc agtaaatgcg agccaggttc cgccggagcg ctaattgaga
     4861 acagcaagaa tacgaagagg gacagcatgg ccttactcca gcagcttgga tgcttcgaga
     4921 atctgctccg ggcgcgcacg aacccagtag ttctccgtca gattcaccca gcgcaccaca
     4981 ccattagcgt ccaggagaaa ttccgccggc cgggcgatgt cctgtccgtt tgcacccgcc
     5041 ccagcatgca gcagatcgta acggcgaatc gcttctgcct ggggatccga aaggaacgtg
     5101 aacgtgtagc cagctttcgt cagcatatcc ctgttgatct ccggcgaatc gacactaatt
     5161 gctactggac gaatccctac ctcagaaagt ttcgcgagat tgcgttcaat accccgtaac
     5221 tcggagtgac aggacggtca ccagtaaccc cgatagaaga ttagcaacac gccacgcggc
     5281 ttcgctgtat tggtgacatt gatgggtgtg gtcagtagtt cagccaggga agttgctttg
     5341 ccactcgcgt cggcgagcgt aaactccggc gccttgttcc ctatgcgcgg cgcgccctgc
     5401 gccggcggca gccaacgtcc cgcaatgaga attgcaaaaa tgaagacgcc aataatcagc
     5461 actccgaggg tggctagcac tgagctgcca actttcgcca gccgtgagcg atcgggggca
     5521 aaggcccgcc gaatacccac aaacagaagc ccggcggcga tcataaacag gatcaggtta
     5581 gcccagggaa aatcgcgggt gatggggaag tcaacgaaga agagcgtgta gctgacagcg
     5641 ccgaaaaagc ttagcaggaa tcccgcccag atgtgccaat tgaatctgcg aatgttcatg
     5701 acaaaggaat attagccgcc attcggtttt ggtcaatggc tgccgggcag cggattaacg
     5761 gacaaacggc ccttgataaa ccagcaatcg gaaactcgat cgtcaatcgg cgttccgcag
     5821 tcgtttgact tgaccgctgt ttcggcttag aaatgctttg tccaaaattc aattcgatga
     5881 aatgaggcct tcgatatgaa gacgcgttcc ttttgcgcca tcctgctttt gctctccctc
     5941 tcgttaatga ttgcttgcat gcaagcgccg ctaactaatc aacctaacgg cgatgctcca
     6001 gtttccacgg cggcggaagc ggcagcactg aaaccggcac ttgattcgat cctggcaaac
     6061 gacatcatgc agcatacgaa ggtgctgtca tcggacgaat acgaagggcg cggaccgggc
     6121 accaagggcg aggagctttc ggtcaagtac ataacggaac agtaccaacg attgggtctc
     6181 aagcccggta atcccgatgg cacctacgtg cagaaagttc cactggccgg ctttacggca
     6241 caaccaactg cgtccttctc tgcggggggc aaaaagattg atttggaatt cccgaaagac
     6301 tatgtcgcgg tctcgcgtcg cttcgttccc gagtcaaaag tcgagaattc cgacattgtc
     6361 ttcgtaggtt atggagtcgt ggcccctgaa tacggctggg acgactacaa gggagtcgat
     6421 gtacgtggca aaacgatcgt catgctgatc aacgatccgg cggtgcctga tacgaccgat
     6481 gccacaaagc tcgatccgaa aatgtttaag ggcaacgcca tgacctacta cggtcgctgg
     6541 gattacaagg tcgccgaagc cgtgcgccgg ggcgcggccg gcgtgctcgt cgtccacgag
     6601 acgaaggcag ccggttaccc gtgggacgtg ccgcgcaatg gcgcgaccaa gccgcagttc
     6661 gacctgcg
//
LOCUS       DASUEX010000105         3823 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_824012, whole genome shotgun sequence.
ACCESSION   DASUEX010000105 DASUEX010000000
VERSION     DASUEX010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3823
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_824012"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..717
                     /gene="dacB"
                     /locus_tag="VFV61_01815"
     CDS             <1..717
                     /gene="dacB"
                     /locus_tag="VFV61_01815"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HEX4899236.1"
                     /translation="LWAAKLFLNTLKARGITVDGDARTRDAREPIADRFEPQRARELT
                     FVSSQPLREIIKATNKGSINLYAELILRTLGRERAGMLPAHEPGSRERGDDEAGLAII
                     RLWLNSSKAPLQGVALHDGSGLSRLNLVMPRTLVQLLAAMQRSPNSQIFRESLPLSGR
                     DGTLGSRLKEYADRVSAKTGYLTYDTALSGFVTTSEGEVLAFSVICNDETERASSGRL
                     IDQIVSRLAAYPSRISEKPQ"
     gene            878..2026
                     /locus_tag="VFV61_01820"
     CDS             878..2026
                     /locus_tag="VFV61_01820"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEX4899237.1"
                     /translation="MFTRISAPIVFAVALLAGGSSLAQSNLSAKVNTLQPRTVGAPVE
                     SDRIKDGLVGPVRRVRTEVVKLVASEGRIVEGKRSLLELVAYDLRGNKIENQYYPVAG
                     ATLTGKETYKYDDKGNISEMILMNDDGSLLSKETYRYEFDFAGNWNKMTTSVAVVDSG
                     RMSFEPTEITYRSIMYYLDENMVKMATAPPATAAVQPNAVPASGNPTSNNSIKTSSEP
                     KPQPPVEVAVDKQPAVNKTAQSVPVSSLNGETLSAGNPVEVKNPGANSTSSPAVVLDS
                     EPPPSAAPKPILRPISGGVLNGSATYLPPPLYPDTAKRMRTAGVVTVEVVIGENGKVI
                     SANATNGPATLREAAIQAALRARFSPTKLSGQPVKVFGTISYKFSLSK"
     gene            2112..3041
                     /locus_tag="VFV61_01825"
     CDS             2112..3041
                     /locus_tag="VFV61_01825"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HEX4899238.1"
                     /translation="MALTGHLSDLSLSELIEFFCNQRKSGRLRVLYPQGSGYFYLQGG
                     SVIHAQIGVLHGIDAVYYALTLANAKFEFSGSADAPERTINQPWTQVVLEGLRRLDEG
                     IAPTEAFPEGYAPDAEDAKPVNKTSANDQAHHSHVPGFLSFMNTDSPKRKPLFAGAVA
                     VALLGSVAAIGVPAGWYSGKAAAVVAEAPAVPVPVSQPEVSPSPESVAVESPATSAAP
                     EDAAALAARKQRERDRAKAREEKAALSAATISPAAAANSSGPKRVSVTVTYDESGRVV
                     QASGGDASALRIARQKRFPPGKAGSATVTIPIN"
     gene            complement(3034..>3823)
                     /locus_tag="VFV61_01830"
     CDS             complement(3034..>3823)
                     /locus_tag="VFV61_01830"
                     /inference="COORDINATES: protein motif:HMM:NF019307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase dimerization domain-containing
                     protein"
                     /protein_id="HEX4899239.1"
                     /translation="GSVGEEGEGNLRGVRHLFNAGKWAGRISAFVSLDGAGLEQITNG
                     ALGSRRYRVQLHGPGGHSWGDFGLANPVHALGRAISSLAAYPAPVDPRTSFNVGRVSG
                     GSSINAIPGEASMEVDLRSISEDELVRLDAFFRRMVREAVEQENSKRRKETPPLELSL
                     NLIGERPSGETPAQSPIVSLAQEATRAVERTPVLERASTDSNVPIALGIPAITLGAGG
                     TSGNQHTIDEWYDPRDRHLGLKRALLVMLGLAGLSPVLNTGKSN"
BASE COUNT          869 a         1113 c         1037 g          804 t
ORIGIN      
        1 ctgtgggcgg ctaaactgtt tctgaacact ttgaaagcgc gcggcattac ggttgacgga
       61 gatgcgcgta cacgagatgc gcgcgagcca attgcagatc gatttgagcc acagcgagca
      121 cgtgagctca cttttgtatc cagccagccc ttgcgcgaga tcatcaaggc cacgaacaag
      181 ggaagcatta atctttacgc ggaactgata ttgcggactt tgggtcgcga gcgtgccggc
      241 atgctgccgg ctcatgaacc tggaagtcgt gagcgaggtg acgatgaggc cggactggca
      301 atcatccggc tctggctaaa tagttcaaaa gcgcccctgc aaggcgtggc gcttcatgat
      361 ggctcgggcc tttcccgtct aaacctggta atgccccgga cccttgtcca gttgctggca
      421 gccatgcagc ggagtcccaa cagccagatt tttcgcgaat cactcccact ttctggtcgc
      481 gacggtaccc tgggctcccg gctcaaagaa tatgcggacc gcgtttccgc caagaccggc
      541 tatctgacat acgacaccgc tttgtcggga tttgttacta cctcagaggg cgaagtccta
      601 gctttttcag ttatttgcaa tgacgaaact gagcgagcca gtagcggacg cttaatagac
      661 cagatcgtct cgcgactcgc cgcttacccg tccagaatct cagaaaaacc ccaataacaa
      721 ctacctaaca gcggtgtttt ccctagacaa acgctgttca actcgttatc attggcctgc
      781 cgcgttccaa tgcggcctca gtgctagcgg ttaagaaccc ctgcctcacg taattcaatc
      841 aacttgtccg acatcctcag agattcagga gttaatcatg ttcacacgca tcagcgcgcc
      901 tattgtgttt gccgtcgccc ttttggctgg tggcagcagc cttgctcaat caaatttaag
      961 cgccaaggtt aatacccttc agccgcgtac tgtcggtgct ccggtggaga gcgaccgcat
     1021 taaggacggg ctggtcgggc ctgtgcgccg ggtgcgcacc gaggttgtta agttggtagc
     1081 atccgaaggt cggatcgttg aaggcaagcg ctcgttactc gagcttgttg cgtacgactt
     1141 gagaggcaac aagattgaga accaatacta tccggtcgct ggagccaccc tgactggtaa
     1201 agagacttac aagtatgacg acaaaggcaa cattagcgag atgatcttga tgaatgacga
     1261 tggttcgctc ttgagcaagg aaacctatag atacgaattc gactttgctg ggaactggaa
     1321 caagatgacg acttctgtcg ccgttgtgga ttcaggaagg atgagttttg aaccgacaga
     1381 gattacttac cgctcaatca tgtactacct cgatgagaac atggtgaaga tggcaaccgc
     1441 cccgccggcg actgccgctg tacagccgaa tgcggttccg gcctctggaa acccgacgag
     1501 caacaactca attaagacaa gttcggaacc caagccccaa ccacccgtcg aagtagcggt
     1561 agacaaacag cccgccgtta acaaaacggc gcagtcggtt cccgtgagtt cgttgaatgg
     1621 cgaaactcta agcgcgggca atccggtcga ggtaaagaac cccggtgcaa actctaccag
     1681 ctctcccgca gttgttctgg attcggagcc tccgccaagt gcggcgccca agccgatctt
     1741 gagaccaatc tccggcggtg tgttgaatgg gtccgctact tacttgccac ccccgttgta
     1801 tccggacacg gcgaagcgca tgcgcacggc aggcgtggtc actgtggaag tggtgattgg
     1861 tgaaaacgga aaagtaatct ccgctaatgc tacgaatgga cccgcgactc tccgcgaagc
     1921 ggcgattcaa gccgcgctac gggcgcgttt ctctcccacc aaactctccg gccagcctgt
     1981 gaaagtattc ggcacgatca gttacaagtt ctccctctct aaataactca gtcgcggcgc
     2041 ctgttaaata cgggcgccgc tgcttttccc cctccaacaa atctctcttc ccgatagtag
     2101 gagctactct catggcattg acgggacacc tcagcgacct ttcactttcc gaactcatcg
     2161 agttcttctg caatcaacgc aaatcgggcc gcctcagagt tctttatccg caagggtcgg
     2221 gatatttcta cctgcagggg ggttcggtga ttcacgcaca gattggcgtg ctacacggaa
     2281 ttgatgccgt ctattacgcg ctcacgctgg caaatgcaaa gtttgaattc agcggttccg
     2341 cggacgcccc ggaacgcaca atcaaccaac cctggaccca ggtggtgctg gaaggattac
     2401 gccgtcttga cgagggaatc gctccgactg aagcgtttcc cgagggctat gctccggacg
     2461 ccgaagatgc aaagccagtc aataaaacct cagcgaatga tcaggcgcac cattctcacg
     2521 tccctggatt cctctccttc atgaacaccg actcgccgaa gcgtaagcca ttgttcgcgg
     2581 gagcagttgc cgtagcatta ctgggaagcg tcgccgccat cggcgtcccc gccggttggt
     2641 actctggcaa agccgccgca gttgtggcag aggcgccagc cgtgcctgtc cctgtgtcgc
     2701 agccggaggt tagtccttcg cccgagtccg ttgcagttga atcgccggca acaagtgctg
     2761 caccagaaga cgccgcggcg ttagcggcca gaaaacagcg tgaaagagat cgcgccaagg
     2821 cgcgtgagga aaaagccgcg ttgagcgcag caacaatttc tccggcagca gccgcgaaca
     2881 gttctggacc caagagagtt tcagtgacgg ttacttacga tgagtctggt cgcgtagttc
     2941 aggcttccgg tggagatgca tctgcgttgc gcatcgctcg acagaaacgc ttccctccag
     3001 gaaaagccgg atcagcaacc gtgacgattc cgattaatta gatttcccag tattaagaac
     3061 aggcgaaagc ccagccagtc ccagcattac caacagcgcc cgtttcaaac caagatggcg
     3121 atcccgtgga tcgtaccact cgtcgatcgt gtgttggttt ccggaagtgc caccggcgcc
     3181 gagtgtgatt gccggtatcc ccagcgcaat cggaacattc gaatcagttg acgctcgttc
     3241 caacaccggc gtgcgttcca cggcccgggt ggcctcctgc gccaacgaca cgatgggaga
     3301 ctgggcgggc gtctcgccgc tggggcgttc accgatcaga ttcagactca gttcgagtgg
     3361 cggcgtctct ttacgtctct tggagttctc ctgctccaca gcttcgcgaa ccatgcggcg
     3421 gaagaaagca tccagtctta ccaattcatc ttcgctgatc gaacgcaggt ccacttccat
     3481 cgaagcctcc ccaggaatcg cattgatact ggaaccgccc gagacgcgtc cgacgttgaa
     3541 actagtgcgt ggatcgactg gcgcgggata ggctgcgagg ctcgagattg cgcgacccag
     3601 ggcgtgcacc gggttagcca gaccaaagtc tccccatgaa tggccacccg gaccgtgtaa
     3661 ttgaacgcga taacgtcgcg agccgagcgc tccgttggta atctgttcca gacccgcgcc
     3721 atcgagactg acaaaagcgg agattctccc cgcccacttc ccggcattga agagatggcg
     3781 aacaccacgg agatttcctt cgccctcttc accgacagaa ccg
//
LOCUS       DASUEX010000106         2059 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_939040, whole genome shotgun sequence.
ACCESSION   DASUEX010000106 DASUEX010000000
VERSION     DASUEX010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_939040"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..668
                     /locus_tag="VFV61_01835"
     CDS             <1..668
                     /locus_tag="VFV61_01835"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX4899240.1"
                     /translation="LPNIGKIIQITAPISHGNSGSPVFNLKGQVVGVVTIKVTNGQNI
                     NLAIGAARVRQLHAGKRRLLAELTMRDRGSDPAESLYKTGLDSLWLGNFDNALGYFEN
                     AVNKNPARADAWVQVGYCKVKQGKNDEAIKAYQQAVLLMPESDEVRNKLGDAYYYSGR
                     LMEAIAAYKEAVRLRPQSAEGYYNLALAYFENGNQQQGLSAARALKQLDERLFARLMS
                     ERD"
     gene            complement(784..>2059)
                     /locus_tag="VFV61_01840"
     CDS             complement(784..>2059)
                     /locus_tag="VFV61_01840"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX4899241.1"
                     /translation="REGNQTVVAGLYIRGGSRNITAENAGIEALMLDVATEASANFPR
                     ERFRRELSRTGTVITYGINYDYAALMLGSTRQHFERGWEIFADAALRPSFAPDDFQRV
                     KQRQLIGLSDDEDTPDSYLQVLLARAAYAGHPYLNDPRGTAKSVAALTVDDLKRYHQQ
                     IMQTSRLLLVVVGDLEVAQVRRMVETSFGKLPRGSYKDVPVPPLSFSASTVTVTPREI
                     PTNYITGVFSAPPLTSPDIYPLRVASSILQNRVFVEVRAKRNLSYAPDAFLGSQAANT
                     GGIYVTAVDANQAVRLMLDEITRLQQEEVTAEELLGTTQHFLTRYYLGQETNGAQAGE
                     LAQAELIGGGWRNSAQFIDRLRAVTAADVKRVSRLYMRNLRFVVLGDPKSIDPNVFTG
                     RPEVGSEFRVPGSEFRVPSSGAQQTQPFGSVR"
BASE COUNT          477 a          571 c          579 g          432 t
ORIGIN      
        1 aactgccgaa catcggcaag atcattcaaa tcacagctcc catctcgcac ggcaatagcg
       61 gcagcccggt atttaacttg aagggtcagg ttgtcggtgt ggtcaccatt aaagtcacca
      121 acggccagaa catcaacctg gcaattggcg ctgcacgcgt gcgacaactt catgctggta
      181 agcgccggct gctggcagag ctaacgatgc gcgaccgggg cagtgatccc gcggagtcgc
      241 tttataagac gggtcttgat tctttgtggt taggaaattt cgacaacgca ctcggttact
      301 tcgagaatgc ggtgaacaag aatccggcgc gggccgatgc ctgggttcag gtcggctact
      361 gcaaagtgaa acagggaaag aacgatgaag ccatcaaggc ctatcagcag gcagtgctcc
      421 tgatgccgga atcagatgag gtacgaaaca aattgggcga cgcttattac tattccggaa
      481 ggttgatgga agcgatcgca gcctataaag aggcggtacg gttacgaccg cagagcgccg
      541 aaggctatta caatctggcg ctcgcgtatt tcgagaatgg aaaccaacaa caagggttaa
      601 gcgccgcgcg tgcgcttaag caactggatg agagactttt tgccaggctg atgagcgaac
      661 gggattaaga agggtttcag attcaaggtt tcaggtttca ggtgtcgggt tcaaggtttc
      721 aggtttcagg tttgaggtct cacgtttgac ccgttaagga caatcgccaa tcattatcgg
      781 tcgctaccgt accgaaccga atggttgcgt ctgttgagcg ccgctactgg gaacgcgaaa
      841 ctcagaacct ggtacgcgaa actctgaacc gacttccggc ctccccgtga acacattcgg
      901 gtcaatactc ttcggatcgc caagcactac aaaccgcaga ttacgcatgt agagacgcga
      961 aacgcgtttc acatccgcag ccgtcacggc tcgcagtcga tcgatgaatt gcgccgagtt
     1021 acgccagccg ccgccaatca attccgcctg cgccaattcg cccgcctggg cgccgttcgt
     1081 ttcctgcccg aggtagtagc gcgtcagaaa atgctgcgtc gtgccaagca gttcttccgc
     1141 tgtcacttct tcttgctgca agcgtgtgat ctcgtcgagc attaagcgga ccgcctgatt
     1201 cgcgtcaaca gcagtaacgt aaatcccgcc cgtgttcgcc gcctggcttc ccaggaaagc
     1261 atccggtgcg tacgacagat tgcgcttcgc ccgcacttcg acaaaaacgc ggttctgcaa
     1321 aatcgatgat gccacgcgca gcggatagat gtcgggagac gtaagcggcg gagctgagaa
     1381 aactcctgtg atgtagttcg ttggaatttc acgcggcgtc acagtcactg tcgacgcaga
     1441 aaacgagagc ggcggcaccg gcacatcttt gtagctcccg cgcggcaact tgccgaatga
     1501 cgtttccacc atccgacgaa cctgcgccac ttccagatcg cccaccacaa ccaacaggag
     1561 tcgcgacgtc tgcatgatct gctggtgata acgcttcaga tcatcaactg tcagggcagc
     1621 gacagacttg gcggtgccgc gtggatcgtt caggtacgga tgtcctgcat aagcggcgcg
     1681 tgccagcagc acttgcaggt aggagtccgg cgtatcttca tcgtcactca agccaatcag
     1741 ctgtcgttgc ttgacgcgct ggaaatcgtc gggagcaaag ctgggccgca gcgccgcgtc
     1801 ggcaaatatt tcccagcctc tttcaaagtg ttgacgcgtg ctacccaaca tcagggctgc
     1861 gtaatcgtag ttgatcccgt aagtgatcac cgtcccggtg cgggacaact cgcgacgaaa
     1921 acgttcgcgc gggaaattcg cgctggcttc agtagcaaca tccagcatca aggcttcgat
     1981 gccggcgttt tcagcggtga tgttgcgcga gcctccgcgg atataaagcc cggcaacaac
     2041 tgtctgattg ccttcgcgg
//
LOCUS       DASUEX010000107         4508 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_939108, whole genome shotgun sequence.
ACCESSION   DASUEX010000107 DASUEX010000000
VERSION     DASUEX010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4508
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_939108"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1034
                     /gene="ftcD"
                     /locus_tag="VFV61_01845"
     CDS             <1..1034
                     /gene="ftcD"
                     /locus_tag="VFV61_01845"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HEX4899242.1"
                     /translation="RDLRLPIADCRFTIFLSSLLQETNLSPTHPIANPQSPIGNLVEC
                     VPNFSEGRNKETIARLSEAIASVETACVLDTHIDPDHNRSVITFVAHPSQVVTAAVKA
                     VALAAELIDMRRHTGVHPRLGATDVLPFVPVAGITLDECAALAHRAGERIARELSIPV
                     FYYEQAALRPERKNLEDVRRGALELLREQIATVPERAPDVGPSRVHETAGAIAVGARP
                     FLIAFNINLRTNDVEIARQIARAVRARQGGLPFVKALGFELATRGLVQVSMNLVNYEV
                     TGMEAAYAAVKSEAERLGVEIANTEIVGLVPEKALDTKAEYFSKLENFEGDKVLENRI
                     EQLCRGTRE"
     gene            complement(1325..2644)
                     /locus_tag="VFV61_01850"
     CDS             complement(1325..2644)
                     /locus_tag="VFV61_01850"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX4899243.1"
                     /translation="MPFVFSRFFYFLILVGLVPLSLSWGRPWLRWATLAYDLILISLA
                     IIDGRYSRLSKGIHISREFAGRFAVGAETDVQIKLHNSTPRPITLRIKDEYPPLMKLI
                     GAREAAVRVAAQTEAILVYGLKPPKRGRFEFGQTAVRFLSRWSLAWCQTKVGEPTAVK
                     VYPNMRRAREAELKALGARSLVAAHRKTSWRGEGREFESLREYVRGDELRHISWTATA
                     RRGRLTTRQYQIERDQTILIALDAGRLMTARIDQETKLDSAVHAALALMSAAARGGDN
                     AGLMVFGRRIQHYLPPSRGREHMDAALEALHAVEPEMIEPSYARALEFVAANSKRRSL
                     VVLLTDLVDEEGSKELLTSLKLLRPRHLPLVVTIADRDLKAVVRDAPETGQELFTQSV
                     AEEIMHLREAALRLVESQGGLALDVTAAALAPALLEKYLQVKERGLL"
     gene            complement(2651..3373)
                     /locus_tag="VFV61_01855"
     CDS             complement(2651..3373)
                     /locus_tag="VFV61_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899244.1"
                     /translation="MSPKKLALWFLIVSVSLSALFGIVAIIRGNFGDFEARVMLTTVT
                     ISAASICALAAGAFWESRSTRTLPGLAVALAVFAAALIIIGIWGKISATPYWKFSVSV
                     GVRAIACAQACLISLARLASRFAWTRTITLLAIFFLAALVIFALYGEVREEGYYKLMG
                     ATAILVAALTIMVPIFHRLSRGDLSSAKPSASGDQHLFPTVLCPRCGMTQSNSSTQIT
                     CSSCGCRFLVTIFSDVPPTQPN"
     gene            complement(3604..4482)
                     /locus_tag="VFV61_01860"
     CDS             complement(3604..4482)
                     /locus_tag="VFV61_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899245.1"
                     /translation="MSRRFSRRYLTAALLLLMATIPPSSTSRAISDEDDLTAEQVIAR
                     HLDSIGSAEARASITSRVILGRALAAARIGGSGQAEGQVVLASRGHNSLVGMAFTVPN
                     YPHEKMGYDGKKLTVAELTPGVRSELGKFFMAHEMPFREGLLSGTLSTAWPLLNLPSR
                     KAALKYEGTKTLQGHKRYVLRYETKNDSGLKTRLYFDAETFRHVRTEYEHRIIQQMAT
                     APGVTQKQGDSITKLVEEFSDFKPVDGLSLPHSYKLQLSVEMLGRRILQDWTLSLSQF
                     VANRTLDDKEFDVVAK"
BASE COUNT         1069 a         1232 c         1211 g          996 t
ORIGIN      
        1 atagggattt gcgattgcca attgccgatt gccgatttac gatctttctt agctcattac
       61 tccaagaaac taacttgtca cctactcatc caatcgccaa tccccaatcg ccaatcggca
      121 atcttgttga gtgtgtacca aacttcagcg agggccgcaa caaagaaacg atagcgcggt
      181 tgagcgaggc gattgccagc gttgaaactg cctgcgtcct ggatactcac attgatccgg
      241 atcacaatcg ttccgtaatc acttttgtcg cgcacccgtc ccaagtggtc acggcggcag
      301 ttaaggccgt cgcgttggcg gccgagctga tcgacatgcg tcggcataca ggtgtgcatc
      361 ctcggctcgg cgctaccgat gtcctcccct ttgttcccgt tgccggtatc acattagacg
      421 aatgcgcggc gctggctcat cgcgccgggg agcgaatcgc acgcgaactc tcaattccgg
      481 tcttctacta cgagcaagcc gcactccggc cggaacgaaa aaacctggaa gacgtgcggc
      541 gcggtgcctt ggagttactt cgtgagcaga tcgctaccgt accggagcgg gcccctgatg
      601 tcggaccttc gcgcgtgcat gaaactgccg gtgcgatcgc tgtcggcgcg cgaccgttcc
      661 tgattgcgtt caatatcaat ttgcgcacga acgatgtgga aattgcgcgt cagatcgcgc
      721 gcgcggtacg agcgcggcag gggggcttac ctttcgtcaa ggctctgggg ttcgaattag
      781 caacccgcgg cctggtccag gtttcaatga atctcgttaa ttacgaagtc accggaatgg
      841 aagctgccta tgcggcggtt aagagcgaag cggaaagatt aggcgtggaa atagccaata
      901 cagaaattgt ggggctggtg ccggagaaag ccctggatac gaaggcggaa tacttttcga
      961 aacttgagaa ctttgaagga gacaaggtgc tcgagaatcg gatcgaacag ttatgcaggg
     1021 gaacgaggga ataggggaag aaaaaaatat ttatcatttc tcatttgtca tttttcattg
     1081 gaagcaacga aaccgccaag tcgccaggga gcgcggagca caccaagaag atgtgacggt
     1141 cagaaaagtt gtgcgacgtg gtcggaataa tcctggatac tgttcatatc ttccgagagc
     1201 ctgacgtact ctgctttccc ttttcggctt ggcggttttg ttttcgatga aaaatgaaaa
     1261 atgtgaaatg acaaatggaa aattgtttcc ctctgtttct cccccttccc ccgttcccct
     1321 gttttcacaa cagccctctt tccttcacct gtaaatattt ctccagcagc gctggggcaa
     1381 gtgcagccgc agtcacatcc aacgcgagac cgccttgtga ctcaaccaag cgcaaagccg
     1441 cctcgcgtaa atgcatgatc tcttcagcta ccgactgcgt gaaaagttcc tgaccggttt
     1501 ccggcgcatc ccgtacgacc gccttcaaat cccgatcggc gattgttacc actagcggca
     1561 gatgtctggg ccgcagcagt ttcaaagacg taagcagttc ttttgaacct tcttcatcaa
     1621 caaggtccgt gagcaacacc acaagggatc tgcgtttact gttcgcggcc acaaactcca
     1681 aggcacgcgc gtaggacggc tcaatcattt ccggctcaac tgcatggagc gcctcgagcg
     1741 cggcgtccat gtgctcgcga ccgcgactgg gtggcaagta gtgttgaatc cgacgaccaa
     1801 agaccatcaa gccggcattg tctcccccgc gcgccgccgc tgacatcagg gcaagcgcag
     1861 catgcacggc cgagtccaac tttgtctcct gatcgatgcg cgcggtcatc aggcggccgg
     1921 catcgagagc aatcagaata gtttggtcgc gctcaatctg atattggcga gtcgtcaatc
     1981 ggccccggcg tgccgtcgcc gtccaggaga tgtgacgtaa ctcgtcgccg cgcacatatt
     2041 cacgcaagga ttcaaactct cgtccttcac cgcgccagga agtcttacga tgagcagcga
     2101 caagggaacg cgcgccgaga gctttcaact cagcttcgcg agcgcgccgc atgttcggat
     2161 aaaccttaac tgcggttggt tcaccgacct tcgtctggca ccacgcaaga ctccagcgcg
     2221 aaagaaagcg caccgccgtc tgcccaaact cgaagcgacc gcgtttcggc ggcttcaatc
     2281 catagaccag gatcgcctcg gtttgcgcgg cgactcggac agccgcttcg cgcgcgccaa
     2341 tcagcttcat cagtggtggg tactcatctt tgatgcgcag tgtaatcggc cgcggagtgg
     2401 agttgtgcag tttgatttgc acgtcagtct cggcgcccac cgcaaagcga ccagcaaact
     2461 ctcgtgagat gtgaattcct ttcgacagcc tgctgtagcg cccatcaata atggcgaggc
     2521 ttatgagaat gagatcgtag gcgagcgtgg cccagcgcag ccagggacgc ccccaggaga
     2581 gtgaaagcgg caccaggccg acgaggatga gaaaatagaa gaatctactg aaaacgaagg
     2641 gcatcgctgt tcagttcggc tgcgttggag gcacatcgct gaagatggtc accagaaaac
     2701 ggcagccaca gcttgagcaa gtgatctggg tagaggagtt tgattgagtc atgccgcacc
     2761 gcgggcagag tactgtcggg aaaagatgct gatcgccaga cgccgatggt ttcgcactgc
     2821 tcaggtcgcc acgactcaag cgatgaaaga ttggcaccat gatagtgagt gccgccacca
     2881 gaatcgcggt ggcacccatt aacttgtaat agccttcctc ccgtacctcg ccataaagag
     2941 caaaaatgac cagcgctgcc agaaaaaaga tcgccagcag ggtaattgtc cttgtccagg
     3001 cgaaccgcga cgccaggcgc gccagggaga tcaggcaggc ctgcgcgcag gcgatagcac
     3061 gcactccgac cgaaacgctg aatttccaat agggagtcgc gctaatcttt ccccagatgc
     3121 ctatgattat taaggcagcg gcgaagactg cgagcgccac tgcgagaccc ggaagagtgc
     3181 gcgtgctgcg actttcccaa aaggcgcccg ccgccaaagc gcagatgctg gcagctgaga
     3241 tcgtgacggt agtgagcatc acgcgggcct caaagtcgcc gaagttgcct ctaatgatcg
     3301 caactattcc gaatagcgca ctgaggctga cggagacaat caaaaaccag agagcaagtt
     3361 tcttgggaga cataatttgg taacaagctg tttgcttacg cgcaaactaa cggtttgcgc
     3421 aacgaatcgg ggagcattcc acaaatcgcc tcgcccgcaa aatgcggagc ataactttgc
     3481 agtcgcaaac tatctgagtt tttatctagc cgcaagtctc attgcttcgt cggcaatctc
     3541 atttgcagag gaatcaagag attcaggcta cctgcgagaa ttggaattga tccacggctg
     3601 actctatttt gcgacgacgt caaactcttt atcgtccaat gttcggttag cgacaaactg
     3661 tgaaaggctc aacgtccaat cctggagaat acggcggccc agcatttcta ccgacagttg
     3721 taatttgtag gaatgcggta acgataaccc gtcgaccggc ttgaaatccg aaaattcttc
     3781 gaccaacttt gttatcgaat caccctgctt ttgagtgaca cccggcgcag tggccatttg
     3841 ttgaatgatt cgatgttcgt actcggtgcg tacgtgtcga aatgtttcag catcaaaata
     3901 gagtctggtc ttcaagcccg aatcattctt agtctcatat cgaaggacat acctcttatg
     3961 tccctgaagt gttttggttc cctcatactt cagcgccgct tttcttgagg gcagattcag
     4021 taggggccag gcggtcgaca gagttcctga cagtaatcct tcccggaagg gcatctcatg
     4081 ggccataaag aacttcccca gctccgatcg cacacccggg gttaattctg caacggtcaa
     4141 cttcttgccg tcatatccca ttttttcgtg cggatagttc ggaacggtaa aggccatgcc
     4201 tacaaggctg ttgtggcctc gagaagcgag cacgacttgg ccttctgcct gacctgaccc
     4261 acctattcgt gctgcagcca gggccctgcc cagaattacg cgactagtaa tggaagcgcg
     4321 cgcttctgca gagccgatcg aatcaagatg tctggcgatc acttgttcag cggttaaatc
     4381 gtcttcatct gatatggctc tcgaagtaga ggatggggga atggtagcca tcaaaagcag
     4441 aagcgcggcg gtcaggtatc tccgcgaaaa tcgtcgtgac attttttact cctttttaat
     4501 ttctgcgc
//
LOCUS       DASUEX010000108         5196 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_595798, whole genome shotgun sequence.
ACCESSION   DASUEX010000108 DASUEX010000000
VERSION     DASUEX010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5196
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_595798"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..775)
                     /locus_tag="VFV61_01865"
     CDS             complement(<1..775)
                     /locus_tag="VFV61_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899246.1"
                     /translation="MTDSIEQTILCVASFFKGNDFIRECKRNGARVVLLTRDKLLTAD
                     WARESIDDLVAIPGKTSVQSYLTAATHIARHRRVSHVVALEEYDIVTAAHIREHLSVA
                     GMGVTTARCFQDKLAMRARARDLGIPQPDFVPLVNFEAIAEFMKRTSAPWMLKPRIGA
                     SAMGIRKLEEPDAVWGTIAELDARAEFQERSAFHLLEHFIPGDVYHVDSLVAGGKILF
                     ANVERYGAPPFDVSHFGGVNLSHTAKRGSKVQRQMLALNK"
     gene            complement(1029..4490)
                     /locus_tag="VFV61_01870"
     CDS             complement(1029..4490)
                     /locus_tag="VFV61_01870"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX4899247.1"
                     /translation="MTKDRLGPAGKLAQAFINSKLTPLVIVASILLGVGAVLLLPREE
                     EPQIVVPMIDVFVQMPGASAKEVEERVTKPMEKLLWEVPGVEYIYSTSAPGYSMAVVR
                     FYVGQSEEASIVRLNQKMSANSDLIPPGASPPLIKPRSIDDVPILALTLASDRYDHFT
                     LRRVAAQVHDQIKEVRDVSEVKIIGGQRRQIRVLLDDARMAAYGAAPASIVPMLAQEN
                     TLLQSGSFSLGNREFLVETGGFLHSAEEVGDVVIGVSNGRPVFLRDVARIEDGAEEPA
                     DYVLYGTGPAKNGATSTTTTPSAAKQNVMPALTISVAKRKGTNAIAIADQVIEKVKHL
                     QGTLIPGDVNVVVTRNYGETASEKSNELLLHMMIAILSVSLLIWIVLGLREAGVVATA
                     IPVTLALTLAVFYFYGYTLNRITLFALIFSIGILVDDAIVVVENITRHYRLPENRERP
                     IMNIAVEAVNEVGNPTILATFAVIAAILPMAFVSGLMGPYMRPIPIGASAAMVFSLLV
                     AFVVTPWASLRLLKRDTGHGDSHDREGWTTRGYRRVMGALIHDRRWRYTFLFCVALLL
                     VGALSLVALKLVRVKMLPFDNKSEFQVIVDMPEGTTLEGTAAVTREIGDYIRTVPEVT
                     DYQMYVGTASPYNFNGLVRHYFLRRGANVADIQVNLVPKDERSAQSHDIAKRVRPAIQ
                     QIATKYLARVKVSETPPGPPVLQTLVAEVYGPNYPRQIEVARQIEAIFDKTEGVVDVD
                     SYIEDPQVKYHFVVDKEKAALNGVSAQQIAATLRIAIEGINVGLTHQPQEKEDVPIVL
                     RLPRAERSSVDDLKQIKVIGLQGSLVSLGELVRVEEQVADQSIYHKNLMPVVYVTGDV
                     AGKVESPVYAILQLNEALDHLQLPEGYGLERYVANQPFSAEKLAMKWDGEWHITYEVF
                     RDLGLAFGAVLILIYILVVGWFQSFKTPLTIMAAIPFSLIGILPAHALMGAFFTATSM
                     IGFIAGAGIVVRNSIILVDFVELRLNQGMALADAVVDAGAVRFRPMMLTAAAVIVGSG
                     VILFDPIFQGLAISLMAGEVASLLLSRMTVPVLFYMSERKKQEVRSASEQLISAPQAP
                     EETNVTSVERPAPGIESVSVVESAAESEPATGNGEGSEAEHDPMPETSDATNGIEDEE
                     SSGSTNQ"
     gene            complement(4504..>5196)
                     /locus_tag="VFV61_01875"
     CDS             complement(4504..>5196)
                     /locus_tag="VFV61_01875"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX4899248.1"
                     /translation="LQALEAKRRQALARIDQAKADVAGSQVYSSFSRIAAPINGVVVS
                     RQADVGYMAAPGAPLLTIESGTDYRLEAAVQESQINNIHLRDQVRVQIDALGPQELAG
                     IVVEVVPTADPASRTYLVKISIAPPDGNQQIIRSGLYGKARFIIGQTQALTIPRKAVV
                     ERGQLTSVYVVDQSGVARMRLVKTGKTYTDRVEMLSGLSEGEQIVIDGVEAVSDGSRV
                     REAAPGLPPATK"
BASE COUNT         1166 a         1496 c         1416 g         1118 t
ORIGIN      
        1 tcttattcaa cgcgagcatt tgccgctgta ctttcgaacc gcgctttgct gtgtgcgaga
       61 gattaacgcc gccaaaatgc gaaacgtcga acggtggtgc cccgtaacgc tcaacgttcg
      121 cgaagaggat cttgcctccc gcaactaacg aatccacgtg atagacatcg cccggaatga
      181 agtgttcaag gagatgaaag gccgaacgtt cttgaaactc tgcccgggca tcaagttcgg
      241 caattgtgcc ccacaccgca tcgggttctt ccagtttcct gatgcccatc gcggatgcgc
      301 caatgcgagg tttcagcatc catggcgcgg aagtgcgctt catgaattcg gcaatcgcct
      361 cgaaattgac caaaggaaca aaatctggtt gcgggatacc aagatcgcgg gctcgggcgc
      421 gcatcgccag cttgtcttga aagcagcgcg cggtggtgac gcccatgccg gccacagaca
      481 ggtgttcacg gatatgggcg gcggtaacga tgtcgtattc ttcgagtgca accacatgcg
      541 acactcgccg gtgccgcgct atgtgggtcg cggcagtgag gtaagactga acactggtct
      601 tccccggaat ggccaccaga tcgtcaatcg actcacgcgc ccaatccgcc gtcagcaact
      661 tgtctctggt taacaaaacc acccgcgcac cattccgctt acattcgcgt atgaaatcgt
      721 tgcccttgaa gaaactggca acacaaagaa tggtttgttc gatcgagtcg gtcataacga
      781 atccagactg ttcatgtttt ttcccctgtc atttgcgatt gaatgaaaag ccgcgcagtg
      841 aaaccaagga catctactta gtgcgcgacg cggattgaat cagcttaata catgaaagac
      901 tgcaacagac atgccgggac ggttgaccat aaaacgttgc gggcattgtc gccccagtga
      961 agggttgggc ggaaagttcc ccttttgttg cgcgaaagct cgcatggttg tgtgcgctgg
     1021 aatctcgctt attgattggt gctgccggac gattcctcat cctcaatccc gttcgttgcg
     1081 tcagacgttt caggcatcgg atcgtgctca gcttcgctac cttcaccatt cccggtagcc
     1141 ggttcacttt ccgccgcgga ttcaaccact gatacggact cgatccccgg cgccggccgc
     1201 tcaacggaag taacgttagt ttcctcaggc gcctgaggag cagagatcag ctgctcgctt
     1261 gcggaccgca cctcctgttt tttccgttca ctcatgtaga aaagaactgg tacggtcatg
     1321 cgagagagca agagtgaagc aacctctccg gccatgagcg aaattgccag accctggaag
     1381 atcggatcaa atagaattac ccccgagccg acaatgaccg ccgcggcggt caacatcatc
     1441 ggacggaaac gcactgctcc tgcatcgacc acagcgtctg caagcgccat cccctgattc
     1501 agtcttagtt ccacgaaatc caccagaatg atcgaattgc gcacgacaat ccccgcgccg
     1561 gcgatgaagc caatcatcga ggtggcggtg aagaaagcac ccattaaagc gtgcgcgggc
     1621 aaaatgccaa tgagcgagaa tggaatcgcg gccatgatcg ttagcggcgt cttgaaagat
     1681 tgaaaccagc ccacgacgag gatgtaaatc agtatgagca ccgccccgaa cgccaatccc
     1741 aaatcgcgga agacctcgta agtgatatgc cattcgccgt cccacttcat cgcgagtttt
     1801 tcggcgctga aaggctgatt ggcgacatag cgttcgaggc catagccttc cggcagttgt
     1861 aagtgatcca gggcttcgtt tagttgcagg atggcataga cagggctttc cacttttccc
     1921 gccacatcac cggtgacgta gacgacaggc atcagattct tatgatagat gctctggtcc
     1981 gccacctgct cttcaacccg cacaagttca cctaacgaga cgaggctccc ctgcaaccca
     2041 ataaccttga tctgcttgag gtcatcaaca ctcgaacgct cggcgcgcgg cagtcgcaga
     2101 acaatcggca catcctcctt ttcttgtggc tgatgagtca ggccaacatt gatgccctcg
     2161 attgcgatac gcagcgttgc ggcgatctgc tgtgctgaaa cgccgttcag tgccgctttc
     2221 tctttgtcca cgacgaagtg atatttgact tgcgggtcct cgatatacga gtcaacgtca
     2281 accactcctt cagtcttgtc gaagatagct tcaatctgcc gggcaacttc aatctggcgc
     2341 gggtaatttg gtccatagac ctctgcaacc agcgtttgca gaacgggtgg accgggtggt
     2401 gtctcagaga ctttgacgcg ggccaggtat tttgtggcga tttgttgaat cgcgggacgg
     2461 acgcgtttgg cgatgtcgtg actctgcgcc gatcgctcat ccttgggcac caggttgacc
     2521 tggatgtcgg ccacgttggc cccgcggcgc agaaagtagt ggcgcaccag gccgttgaag
     2581 ttgtaaggcg aagcagtgcc gacgtacatc tgatagtcgg tcacttctgg cacagtgcga
     2641 atgtaatctc caatctctcg cgttaccgcc gcggtccctt cgagcgtggt gccctcgggc
     2701 atatccacaa tgacttgaaa ctcgctcttg ttatcaaacg gaagcatctt tacgcgcaca
     2761 agcttcagcg caacgagcga gagcgcgccc accagcaaca acgccacgca aaagagaaac
     2821 gtatagcgcc agcgccgatc gtgaatcagc gcgcccatta ccctgcgata gccacgcgtc
     2881 gtccagcctt cgcgatcgtg agaatcgcca tgaccagtat cccgcttcaa tagtcgcaag
     2941 ctggcccacg gcgtcaccac gaacgccacc aggagcgaga agaccatcgc ggctgacgcg
     3001 ccaatgggaa ttggtctcat gtagggcccc atcaagccgc tcacaaaggc catcggaaga
     3061 atagccgcaa tcacagcgaa ggttgccaga atcgtcggat tgccaacttc gttcacggcc
     3121 tcgacggcaa tgttcatgat aggccgctcg cgattctctg gcaggcgata gtgacgagtg
     3181 atgttctcta ccaccacgat ggcgtcgtcc accagaatgc caatcgagaa gatcagggcg
     3241 aaaagcgtga tgcggttgag ggtgtaacca tagaaataga agacggcaag ggtgagcgcg
     3301 agcgttacgg gaatagcagt cgcgacaacc ccggcttccc gcaaaccaag gacgatccag
     3361 atcaacagtg agacagacag gatggcaatc atcatgtgca gcagaagttc attagacttc
     3421 tcgctcgctg tctcgccgta gttgcgggta actactacat tgacgtcgcc tggaatcaga
     3481 gtgccttgca agtgcttgac cttctcaatc acctgatccg cgatcgcgat cgcattagtg
     3541 cctttgcgct tagctaccga gattgttagc gccggcatta cgttctgttt agccgctgac
     3601 ggagtagtgg tggtggatgt agccccgttc tttgccgggc cagttccata cagaacatag
     3661 tcagccggct cttcggcgcc gtcttcaatt cgcgccacat cgcgcaggaa cacggggcgg
     3721 ccgttggaaa cgccgatgac cacgtcgccg acttcttctg cggaatgcag gaagcccccg
     3781 gtttctacta gaaactcgcg gttgcccagg gaaaagctgc cagactgcaa cagtgtgttt
     3841 tcctgcgcga gcatcgggac aatgctcgcc ggagcggcgc cgtaggcagc catgcgcgcg
     3901 tcatccagca gcacgcgaat ctgacgacgc tgcccgccaa tgatcttcac ttcagagacg
     3961 tctctgactt ctttaatttg atcgtgaacc tgcgcggcaa cgcgtctgag ggtgaagtgg
     4021 tcgtagcgat cactggcaag agtgagggcc agaatgggca cgtcgtctat cgagcgcggc
     4081 ttgatgagcg gcgggctggc gccgggcggg atcagatcag agttggcgga catcttctgg
     4141 ttgagccgga cgatgctcgc ttcttcactt tggccgacgt aaaagcggac gaccgccatt
     4201 gaataaccgg gtgccgaggt cgaataaatg tactcaaccc cgggaacttc ccagagcaat
     4261 ttctccattg gcttcgtcac ccgctcttca acttctttgg cagatgcgcc cggcatctgc
     4321 acgaagacgt cgatcatggg cacgacgatc tgcggttctt cttcgcgagg gagcaggagc
     4381 actgcgccga ctcccagcag gatggaagcg acaatgacaa gcggcgtcaa tttggagttg
     4441 atgaatgcct gtgccagttt gccggcggga ccaagtcgat ctttcgtcac tcttccacct
     4501 cgactacttc gtcgctggcg gcaaacccgg agcggcctct cgcacccggc tgccatcact
     4561 taccgcttcc acgccatcaa tcacgatctg ctcgccttcg cttaatcccg aaagcatttc
     4621 cacccggtca gtgtaggtct tgccggtctt cactaatctc atgcgcgcga cgcccgactg
     4681 gtcgaccaca tagacactgg taagttgtcc acgctcgacg acagcttttc ggggaatcgt
     4741 gagggcttgc gtctggccga tgatgaagcg ggctttacca taaagcccag agcgaatgat
     4801 ttgctgattg ccgtcgggcg gtgcaatgct gatcttgacc agataagttc tgctcgccgg
     4861 atcagcagtt ggaacaacct cgaccacgat gcccgcaagt tcttgcggac ccagggcatc
     4921 tatctgaacg cggacctgat cccgtaaatg aatgttgtta atttgggatt cttgtacggc
     4981 ggcttcgagc cggtaatcgg tcccactttc gatcgttagc aaaggcgcgc ctggtgcggc
     5041 catgtaaccg acatccgctt gccgggaaac aacaactccg ttaatgggcg cagcaattcg
     5101 ggagaagctc gaataaacct gagagcccgc gacatcagcc ttagcctgat caatacgggc
     5161 cagcgcctgt cgccttttcg cctccagcgc ttgcag
//
LOCUS       DASUEX010000109         3135 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1511828, whole genome shotgun sequence.
ACCESSION   DASUEX010000109 DASUEX010000000
VERSION     DASUEX010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3135
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1511828"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1267
                     /locus_tag="VFV61_01880"
     CDS             <1..1267
                     /locus_tag="VFV61_01880"
                     /inference="COORDINATES: protein motif:HMM:NF021606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HEX4899249.1"
                     /translation="TVKILFTLTGNAVALTLLSVDLNDYVSARLSNGADLTNLNAQIE
                     NARQFSISVVWTLYAATMLVLGLLRRSRLLRWGGLLVLLTAIGKVVVLDSTYYAAWWH
                     FPIFNQTFMAYALLVAVLAFSARLYGRPGSANESERRVMLPALLLAANLFALAALSLE
                     VIGYYDRSMASLLLSTSSVEVFNPFQEGKIFTLALIWTLYATCAFMFGAWRHSRVWRY
                     GGLLLLAFTAPLVLANLSYYDASWHILILNRTLAGFAILIAALWLVVRTYARSGDAFE
                     EAYIVRPVATVAGNLLAIVALSAQAAGYYEAKIFEELTHGTAAASGFEGFEGPRNLEL
                     AKQLSLSVVWGLYACGLFVAARVRRLRLLRVMGLVLLSLTTLKVFLVDLSSLERGYRI
                     FSFIMLGAILLVVSYFYQRSQQRAAVKES"
     gene            complement(1402..1542)
                     /locus_tag="VFV61_01885"
     CDS             complement(1402..1542)
                     /locus_tag="VFV61_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899250.1"
                     /translation="MPLSKYSSSDIRVSRPSGSIVRFLWKTLGFYLALVGLGLIGEAV
                     ID"
     gene            complement(1626..2720)
                     /locus_tag="VFV61_01890"
     CDS             complement(1626..2720)
                     /locus_tag="VFV61_01890"
                     /inference="COORDINATES: protein motif:HMM:TIGR03718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEX4899251.1"
                     /translation="MVLPRVRWTCSLTAGARPGVLHRKAHRVGFREAATWSLVWISLA
                     LLFCFLLYQYALWKFPQDPRLMAVPGFDPEAAAWLVSLEFLTGYVVEKSLSVDNIFIF
                     VMVFSYFAIPAKYQHRVLFYGILGALVFRAIFVALGSALMQYHWVVLFFGAFLIFTGI
                     KIFFAKEKEVEPEKNLLIRLLRRLVPVTHELQGRQFFARRDGRLYATPLLVALVFLEA
                     TDIVFAVDSVPAIFALTNEPLVVFTSNIFAILGLRALYFMLAGAVDKFHLIKYGLGIV
                     LIFVGLKMVWLNELFGGKFPIGISLGIIAVVIGASVVLSLAFPKAVTEVGAESSPASG
                     TSTEISQLSLTEEVLTPIEATETQKKRARI"
BASE COUNT          704 a          869 c          869 g          693 t
ORIGIN      
        1 aacagtcaaa attctcttca cgctcacggg caacgccgtg gcgctgacgc ttttgtcggt
       61 cgacctgaac gactacgtca gcgcgcgcct gtcgaatggt gcagacctaa cgaacctaaa
      121 cgcgcaaatc gagaacgcga ggcagttctc gatctccgtg gtctggacgc tttatgcggc
      181 gacgatgctg gtgttaggac tgctgcggcg gtcccggctg ttgcgctggg gtgggttgct
      241 tgtgttgctg acggcaatcg gtaaggtagt cgtccttgac tccacctact acgcggcgtg
      301 gtggcacttt ccgatcttca atcagacctt tatggcctat gcgttactgg tcgcggtgct
      361 cgccttcagc gcgcgtctct acggacgacc gggaagcgcc aacgaatcgg aaagacgcgt
      421 gatgctcccc gcgctgctac ttgcagccaa cctgttcgcg ctagcggcgc tcagccttga
      481 agtaattggc tactacgacc gctcgatggc cagcctcctc ctcagcacgt ccagcgttga
      541 agtcttcaat ccgtttcagg aaggaaagat cttcacgctc gcgctgattt ggactctcta
      601 cgcgacgtgc gcgttcatgt ttggcgcgtg gcggcactcg cgagtgtggc gctatggtgg
      661 actgttgctc ctggcgttca cggcgccatt ggtcttggcg aacctgagtt actacgacgc
      721 gtcatggcat attttgatcc ttaaccgaac gctggctgga ttcgcgatcc tgatcgcggc
      781 cctttggctt gtggtgcgaa cttacgcgcg ctcgggtgac gccttcgagg aggcctacat
      841 agtgcggccg gtcgcgacgg tggctggcaa cctgcttgcc attgtcgcgc tcagcgcaca
      901 agccgccgga tactatgaag ctaagatttt cgaggagttg acccacggga cggctgcggc
      961 gtcagggttt gagggctttg agggtccgcg caatctggag cttgcgaagc agctttcgct
     1021 ctcggtcgtg tgggggctgt atgcctgcgg cttatttgtt gccgcccgag tgcgccgcct
     1081 gcgtttgctg cgagtgatgg ggctcgtact cctctcgttg acgaccctca aagtgttttt
     1141 agtggacttg tcttcgcttg aacgcggcta ccgaattttt tccttcatca tgctgggagc
     1201 gatcctgctc gtagtttctt acttctacca gcggagccag cagcgcgctg ccgtcaaaga
     1261 gagttgagtg ttcggatctt gtttacaaaa ttactgattc cgctgacaag ttcaggggcc
     1321 accggcagct tgggatcact gtacgatgtt acttgctttt ccctttcatt cccgaccgtc
     1381 ttgagaacat gattcattcc ctcaatcaat aacagcttcg ccgattagac cgagtcccac
     1441 cagcgcgagg tagaagccga gcgtcttcca caggaaccga actatcgagc cggacggcct
     1501 acttacccgg atgtctgacg aactgtattt gcttagtggc atagttgcga aacgttctcc
     1561 gttagacgat ctttgactgg ccactgcggg agaattgtcg atttggcgtc cactcttcgt
     1621 cctcactaaa ttcgtgctcg cttcttttga gtctcagttg cctctattgg tgtgagcact
     1681 tcctcagtca gactaagttg cgagatctcg gtgcttgttc ccgacgcggg actcgattct
     1741 gcgccgacct cagtcactgc tttggggaaa gcaagtgaaa gtacaaccga agcgccgata
     1801 acgactgcaa tgatcccgag tgaaataccg attgggaact tgccgccgaa caactcattg
     1861 agccagacca tctttagtcc cacgaagatc agaactatcc ccaagccgta cttgatcagg
     1921 tgaaacttat ctaccgctcc ggccagcatg aaataaagcg cccgcaggcc aaggatggcg
     1981 aagatgttcg aggtgaagac aacgaggggc tcattggtca aagcaaagat tgccggaact
     2041 gaatccaccg cgaagactat gtccgtcgcc tccaggaaga ccaacgccac cagtaagggg
     2101 gtagcgtaaa gtcgcccatc tcgccgggca aaaaactgcc gaccctgaag ttcatgagtt
     2161 acggggacga gtcggcgcag cagtcgaatt agcaaattct tctctggttc gacctctttc
     2221 tccttagcga agaagatctt gatgccggtg aagatcagaa aggcgccgaa gaacaagact
     2281 acccaatggt actgcattag ggctgagccc agtgcaacga atatcgcccg gaaaacgagc
     2341 gccccgagaa tgccatagaa gaggactcgg tgttggtact tcgctgggat agcgaagtag
     2401 ctgaagacca ttacaaaaat gaagatgtta tcgacggaga gcgatttctc aaccacgtaa
     2461 ccggtcagga actccagcga gacaagccac gctgccgcct ctggatcaaa cccaggcacc
     2521 gccatcaagc gcgggtcctg cggaaacttc cagagcgcgt actgataaag aaggaaacaa
     2581 aagaggagcg ccagactaat ccatacgagg ctccaagtcg cagcctcacg gaacccaacc
     2641 ctgtgtgcct tacgatggag gaccccaggt cgagcgccag cagtaagaga acaagtccaa
     2701 cgaacgcgag gtagaaccac caatactcgg agagcgggaa aagctgaatc atgaccctgt
     2761 cctttcgcta gagcggatgt gacttccagc agccacgcta aaaggaaaaa accctttgcg
     2821 tgccgcgtga gccgcaaaag gtcttggtca ttgagctgga tgtgctcttc ccgatgcgcc
     2881 gggtgccttt acgtcaggca ccgtgttgac ggcttcaccg aacccattga aggtgggcga
     2941 cctactcccc tttcgagggt taccatctta caaccgacca caggccgagt acagccgcac
     3001 cgcgggagac aatccgaccg taacggtcac gatgcaaaag atgtagtttg aaggcgaggt
     3061 aaaggattgg aattacaagg atcgctaggt ccgaatagat tcaaacgact gcccaccacg
     3121 acgtgcccaa actgc
//
LOCUS       DASUEX010000110         2291 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1390776, whole genome shotgun sequence.
ACCESSION   DASUEX010000110 DASUEX010000000
VERSION     DASUEX010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1390776"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /locus_tag="VFV61_01895"
     CDS             <1..448
                     /locus_tag="VFV61_01895"
                     /inference="COORDINATES: protein motif:HMM:NF021942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HEX4899252.1"
                     /translation="DTEGTKLYPDATFTLRLSYGAVKGYMENGKFVPPFTTLGGLYSR
                     AAKFKKFPWVLPPRWIEQKSKVNLATQFNFVSTNDIIGGNSGSPTINKNRELVGLIFD
                     GNIQSLVGNFDYDESVNRSISVDSRGMLEVLRNVFGADAVVAELTR"
     gene            complement(566..>2291)
                     /locus_tag="VFV61_01900"
     CDS             complement(566..>2291)
                     /locus_tag="VFV61_01900"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEX4899253.1"
                     /translation="KQQNICVVGDEDQSIYKWRGADITNILNFEQHFPDTKIIRLEQN
                     YRSTQTILDVAGAVVKNNTERKGKKLWTSNPAGDRVRYYQAFDAESEARFVSSKILEH
                     RQEQYDLRSAVLYRTNSQSRVFEEAMRRAGLQYNIVGGFSFYERMEVRDIVAYLKLAL
                     NPNDSIALARVINSPPRGIGKQTVEEIDRRAAEASVTPWEAISLIVENPGSLGSRAIS
                     ALKSFRKLIGDLGEMAGGPGRGDGETGGRGDAETQGRGDAVSEESVSDIVKAAILKSG
                     YENALKSEATDEAEARLENLQELVNAAVDYDDQGISGLRDFIDHSALVSDTDQYKGDA
                     PVTLMTAHSAKGLEFPLVFIVGLEDGLFPHSRSATDPAEMEEERRLCYVAMTRAERFL
                     YVTHAMKRRVYGEELPSEPSQFLNEMPLDLMEDLTRGNSWLSFARGSAAIDYPSSEPR
                     KERKKYEGKTYDSIDSIAEFFKQRGQQFGQASGSSRPAGGYGRKETSFGKAASSSSRE
                     TRNSKLETAVPAAGEFAPGSYVRHAKYGRGLVLRREGVGDGLKLTVTFPGYGQKKLVQ
                     KYANLEKG"
BASE COUNT          489 a          661 c          607 g          534 t
ORIGIN      
        1 cgataccgag ggtactaaac tctatcccga tgccacgttc acgctgcgcc tgtcgtacgg
       61 ggccgtgaaa ggctacatgg aaaacggaaa gttcgtgccg cctttcacga ctctgggcgg
      121 actgtattcg cgagctgcga agtttaagaa gtttccctgg gtgttgccac cgcgctggat
      181 tgaacagaag tcgaaagtta acctggcaac gcagtttaat ttcgtttcca cgaacgacat
      241 catcggtggc aacagtggtt cgccgacgat caacaagaac cgggaactgg tgggtctgat
      301 cttcgacggc aacattcaat cgctcgtcgg caacttcgat tacgacgaat cggtgaaccg
      361 gtcgatctcg gtcgattcgc gcggcatgct ggaagtgcta cgaaatgttt tcggcgccga
      421 tgcggttgtc gcagaactga ctagataaat ttccagaagt ccaaggtcta aggtccaaag
      481 tccaacgtcc gttctcggac gttggacttt tcagtgtagg ccagctgtta gcttgcactt
      541 gcaaccaacg gttttcgtta cagcttcagc ccttttccag gttggcatac ttctgtacca
      601 actttttctg cccgtatccg ggaaacgtca cagtcaactt caaaccatcg cctacgcctt
      661 cgcgccggag caccaggcca cgtccgtatt tggcgtgacg cacataggag ccaggagcga
      721 actcgcccgc ggcgggcact gcggtttcga gtttcgagtt tcgcgtttcg cgcgacgagc
      781 tcgaggctgc ctttccaaaa ctagtttctt tgcggccata accgcccgcg gggcgagagc
      841 tgccgcttgc ctggccaaac tgttgcccgc gttgcttgaa aaactcggca atggaatcaa
      901 tgctgtcgta agttttgccc tcgtatttct tgcgctcttt ccgtggctcg cttgaagggt
      961 aatcgatagc cgccgaaccg cgcgcaaagg agagccagga gttgccacgt gtcaggtcct
     1021 ccatcaaatc gagcggcatc tcgttaagaa actgtgaagg ctccgagggc agctcttcgc
     1081 catagacccg ccgcttcatc gcatgcgtga catacaggaa tctctcagcg cgcgtcatcg
     1141 cgacatagca aagccgccgc tcttcttcca tctcggctgg atcggtggct gagcgagaat
     1201 gcgggaaaag cccatcttcg aggcccacaa tgaacaccaa gggaaactcc aaacccttcg
     1261 ccgaatgggc cgtcatcagg gtaaccgggg catcgccttt gtattgatcg gtatcggaaa
     1321 caagcgctga atgatcgatg aaatctcgca agcccgagat tccctgatcg tcatagtcaa
     1381 cagcggcgtt aacaagttct tgtaagttct ccaaacgggc ttctgcttca tcagtggctt
     1441 ccgacttcag ggcgttttcg taaccgctct tcaatatggc ggccttgacg atgtccgaga
     1501 cggactcttc tgagacggcg tccccgcgtc cctgagtctc cgcgtctccg cgtccccccg
     1561 tctccccatc tccgcgcccc ggcccgccgg ccatctcgcc caaatcccca attagtttgc
     1621 ggaaactctt gagcgcggaa atcgcgcgag agccaaggct cccgggattc tcgacgatca
     1681 aggagatcgc ttcccagggc gtgactgacg cttccgcggc gcgacgatcg atttcttcga
     1741 ctgtttgctt gccgataccc cgcggcggac tgttgataac tcgggcgagc gcaattgaat
     1801 cgttcggatt caaggcgagc ttgagatacg cgacgatgtc tcgcacttcc atgcgttcgt
     1861 aaaacgaaaa accaccgacg atgttgtact gcagtccagc gcggcgcatc gcttcctcga
     1921 agacacgcga ttgagaatta gtgcggtaaa gcacggcact gcgcagatcg tattgctcct
     1981 ggcggtgttc aagtattttc gacgaaacga agcgcgcctc tgattccgca tcaaatgcct
     2041 ggtaataacg aacgcgatcc ccagcgggat tggaggtcca gagcttcttg cccttgcgct
     2101 cggtgttgtt ctttaccacg gcaccagcga catcaaggat cgtttgcgtt gaacgatagt
     2161 tctgttccag acgaattatc tttgtatccg ggaagtgctg ttcaaaattg agaatgttgg
     2221 taatgtcggc gccgcgccac ttgtagatcg actgatcctc atcccccacc acacaaatgt
     2281 tctgctgctt c
//
LOCUS       DASUEX010000111         4206 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_710642, whole genome shotgun sequence.
ACCESSION   DASUEX010000111 DASUEX010000000
VERSION     DASUEX010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4206
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_710642"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2547
                     /gene="lptD"
                     /locus_tag="VFV61_01905"
     CDS             <1..2547
                     /gene="lptD"
                     /locus_tag="VFV61_01905"
                     /inference="COORDINATES: protein motif:HMM:NF016348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HEX4899254.1"
                     /translation="NSGGLHTRLRAATRRIIFQFGLITLSGILLSVAAPALAQQTNPV
                     DRKVTNPMTDTPNVNPLVQDQPVRPRLPPRRGAQGEATEDLKIDAGTQTVSGPPNAQV
                     FVYEGNVDARIGTYRLQADKVTVYEATNKVVAEGNVVFDQSDQQRITGSRAEWNYRTK
                     TGFFVDSTGFTNQTEDGTRIYFTADRVEKISLDTIVATNAQVTACDEDVPKWSFHASR
                     VKIKTGDRVRAYSPTMRIKGVPMLYLPYTSISIKRRDRASGFLTPTFGGSGAKGARFS
                     GAYYKTLGRSADATFRTDIYTSRGIGIGADVRTRANSRSYLNFGFYSVKDRILGSKAD
                     ATHPDQGGSSFYVEGVHYFPNGFIAAADVNITSNLAYRQVFSDNIQQAISPEERSQVF
                     VNKNHGSYSFNFLARTQVTSLPTSRIRIRELPSISIEKRPGLLSFLKRLPVYFSFEGT
                     AGGMSRKETVEDLIAFRAEVGGEPIISPSLVQRLDFHPRVQVPLTFAGFNITASASSR
                     VTFYSNQLDPTTRSVLSQNLTRGYGEFELDFRPPAFARDFHRGDAFFFRHVIEPYIIY
                     RRIYGINEFDRLIRFDYNDAIANTNEIEFGISNRFFTRRSTENVSSAAARAASRGELT
                     PLSSQPYEALTITIRGKYFFDPFFGGALLPGQRNQFYPINTLSGFSYGGFPRRFSPIN
                     IEARYRPSNNIFVDLRTDLDTRDGGLRAISTTFGINRPLVQAFQSFYYTRAIALVGDL
                     SRFGDANGNEPGTQRGSQWSPSIFLGNRERGLFGGASFFFDFQKRPGRGTDSLISSTV
                     TLGYTWDCCAVTVQNYTFDVGLRKENRFVFSFRLNGIGTFGTEQIGQNFR"
     gene            2798..>4206
                     /locus_tag="VFV61_01910"
     CDS             2798..>4206
                     /locus_tag="VFV61_01910"
                     /inference="COORDINATES: protein motif:HMM:NF013580.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallo-peptidase"
                     /protein_id="HEX4899255.1"
                     /translation="MPKKLRLLVFLLAALFLAASFFSLDLTRPSHAAQNGAVLVKDSR
                     ADAQDLDKLLRRYDKLELDPAIVAAQVQETGRIFLPTSTGGFDIRLAPHDMRADRYRS
                     QESLAGGVILELERGPVRTYKGVIAGLPNAQARLTVDKETFEGLIITPGQIYFVEPAK
                     RYSSSAGPRDFVVYQQSDLLQGSFGECGVTTLAEKVGSEALRVQSQTGKVVSGDGIAE
                     ELFTPPRIVELATEADFEYFTFFGNATAANNEIISIMNQVEGIYDSTFGLQFTIVFQN
                     VWSTAGDPYTSTAASTALGEFTNYWNTNHGSSAGPGFVPRDLAHMWTGKSFDGTTIGI
                     AWRPGLDCPLAQNGYGMSERLSSTPGKFILTAHEIGHNFNAEHADAQPNCANTIMGTS
                     LGPSTSQTFCPFSINQIETQANAKIACLAQALTPGCTYSLSSFGQSVGSSGGSGSVNV
                     NTVGTNCAWAANSAVSWII"
BASE COUNT          915 a         1223 c         1072 g          996 t
ORIGIN      
        1 aattcgggag gtcttcacac tcgtttgcgc gccgcgaccc gcagaatcat cttccaattc
       61 ggactaatca ccctcagcgg aattctactg agtgtcgctg cgcctgcact cgcacaacaa
      121 accaatccgg tcgatcggaa agttacgaat ccgatgaccg acactcccaa cgtcaatcct
      181 ctcgtacagg atcagcctgt ccgtccgcgc ttacctccgc ggcgcggcgc gcaaggcgaa
      241 gccactgaag acctgaagat cgacgccggc acacaaactg tctccggccc gcctaatgca
      301 caagtgttcg tctacgaagg taacgtggat gcacgcatcg gcacctatcg cttgcaggct
      361 gacaaggtca cagtttacga agccactaac aaggtggtcg ccgaaggcaa tgtggttttc
      421 gatcagagcg atcagcaacg catcaccggt tctcgtgctg agtggaacta ccgcaccaag
      481 actggcttct ttgtagattc aactggcttc acaaatcaga ctgaagacgg cacacgcatc
      541 tacttcaccg ccgatcgggt tgagaaaatc agcctcgaca ccatagtggc aactaacgcc
      601 caggttactg cctgcgatga agacgttccc aaatggagct tccatgcgtc tcgggtcaaa
      661 ataaaaaccg gggatcgcgt gcgggcctat tcgcctacga tgagaatcaa gggcgtgccc
      721 atgctctact tgccgtacac ctcgatttcc atcaaacgtc gcgatcgcgc ttccggattt
      781 ctgacgccga cgttcggtgg gtcgggggcg aaaggggcaa ggttctccgg cgcctactac
      841 aagactctgg gacgttccgc agacgccact ttccgcacgg acatctacac ctcgcgcggc
      901 atcggcatcg gagccgatgt gcgcacccgc gcgaactccc gctcttatct aaactttggt
      961 ttctattcgg tgaaggaccg catccttggt agtaaggccg acgccacgca tcctgatcag
     1021 ggcggctcga gcttctatgt tgaaggtgtc cattattttc cgaatggttt cattgccgcc
     1081 gccgatgtga acatcacttc gaatctcgcc taccgccagg ttttttccga caacattcag
     1141 caggcaatct cgcccgagga acgctcacag gtttttgtaa acaagaatca cgggtcttac
     1201 agtttcaact tccttgcccg cacgcaggtg acatccttgc cgacttctcg aattcgcatt
     1261 cgcgagttgc ctagtatctc gatcgaaaag cggccgggtt tactcagttt tctgaagcgc
     1321 cttccggttt atttttcctt cgaaggcact gccggcggca tgagtcgtaa ggagaccgtc
     1381 gaagatttga tcgctttcag agctgaagtt ggcggcgagc cgatcatttc accatcgctg
     1441 gtacagcgtt tggactttca tccgcgcgtt caggtgccgc tgacatttgc cggtttcaac
     1501 atcacggcgt cagccagttc tcgcgtgacc ttctactcaa accaactcga tcccacgacc
     1561 cgctcagttt tgagtcagaa cctcactcgg ggttacggcg agtttgaatt ggattttcgt
     1621 cccccagcat ttgcgcgaga ctttcaccgg ggcgatgctt tcttcttccg tcatgtgatt
     1681 gaaccttaca tcatctatcg gcgcatctat ggtattaacg aattcgatcg cttgatccgg
     1741 ttcgactaca acgatgccat tgccaacacg aacgaaatcg agtttggcat ttctaatcgt
     1801 ttcttcacgc ggcgctccac ggagaatgtc agcagtgcgg cagcgcgcgc tgccagcaga
     1861 ggagagctca ccccgctctc tagccaaccg tatgaagcct tgacgattac gattcgtggc
     1921 aagtacttct ttgatccgtt ttttggcggc gccttgcttc ccggccagcg aaaccagttc
     1981 tatccgatca atacactttc cggcttcagc tacggcggtt ttccgcgacg cttttcgcca
     2041 atcaacatcg aagctcgtta tcgtcccagt aacaacattt tcgtcgacct gcggaccgac
     2101 cttgatacgc gcgacggcgg cttgcgtgct atctccacga cctttggcat caaccgtccc
     2161 ctcgtgcagg cttttcagag cttctactac actcgtgcca tcgcactcgt aggcgacctc
     2221 tcgagatttg gcgacgccaa cggaaacgaa ccgggtacgc aacgcggctc gcagtggagt
     2281 ccctcaatct ttctcggcaa tcgcgaacgg ggattgttcg gcggcgcgtc gttctttttc
     2341 gatttccaga agcgaccggg gcggggaacc gactcgctaa taagctcgac cgttacgctt
     2401 ggctatacct gggattgctg cgcggtgacg gtgcagaact acacattcga tgtgggattg
     2461 cgtaaggaaa accgcttcgt attcagcttc cgcctcaacg gcatcggcac ttttggcacc
     2521 gagcaaatcg ggcaaaactt ccgctagccg cagccctttt cagacagcgc tcccactgag
     2581 ttatgctgtt aaagttggcc ttccgccgga cctttttttg gctagccggt tccccgacct
     2641 gaggtttgtg ataaattcac tatggtcgcc ccacctgccg ccgcaggatc aaagcctcaa
     2701 ccagtggaaa ctcgataggt tcgctgccag gctgatgcgc ggctgtcgca cctcccggca
     2761 agattatccc ttaacaaacg ttggttcgga gaaagctgtg ccaaaaaaat taagactcct
     2821 cgtgttctta ctcgctgcgt tgttccttgc tgcgagcttc ttctcactcg atctaacccg
     2881 tccttcccat gccgctcaga acggagccgt gctcgtaaaa gactcccgag ctgacgcgca
     2941 ggatctagac aagctcttgc gacgctacga caagctggaa ctcgacccgg caatcgtggc
     3001 cgcgcaggtt caggagacgg gacgaatctt tctaccaact tcgactggcg gatttgatat
     3061 cagacttgcg ccacacgata tgcgcgcgga ccgctaccgc tcgcaggagt ctttggctgg
     3121 tggtgtgatt ctcgagctcg agcgtggccc ggtgcgcact tacaagggag tgatagcagg
     3181 actgccgaat gcccaggctc gtctcactgt ggataaagaa actttcgaag gactgatcat
     3241 cacacccggc cagatctatt ttgtggagcc ggccaagcgc tattcatctt ccgccggccc
     3301 tcgtgatttt gtcgtttatc aacaatccga tctgctgcaa ggatcttttg gggaatgcgg
     3361 agttaccact ctcgcggaaa aggttggcag cgaagcgctt cgggtgcaaa gtcaaaccgg
     3421 gaaggttgtg agtggggatg gcattgccga agaattgttc acaccgccgc gcatcgtcga
     3481 gcttgccacc gaagcggatt ttgaatactt cacttttttc gggaacgcaa ccgccgcgaa
     3541 caatgagatt atcagcatca tgaatcaggt ggagggtatt tacgacagta cgtttggtct
     3601 ccaattcacg atcgtcttcc agaacgtttg gtcgaccgca ggtgatcctt acacctcgac
     3661 agctgcctcg accgcgttgg gtgagtttac gaactactgg aatacgaatc acggatcgag
     3721 tgcagggcct ggctttgtcc ctcgagacct cgcgcacatg tggacaggga agagctttga
     3781 tggaactacc atcggaatag cctggcgccc aggcctggat tgtcctttgg cccaaaacgg
     3841 ctatggcatg tctgaacgat taagttcgac acccggaaaa tttatcctca cggctcacga
     3901 aattgggcac aacttcaatg ccgagcacgc cgacgctcag ccaaattgtg cgaatacaat
     3961 catgggcacc tcgcttggcc ccagcacctc tcaaacattt tgtcccttct caattaatca
     4021 aatcgagaca caagcaaacg ctaagattgc gtgtctggcg caggcactta cacctggttg
     4081 tacctactcg ctttcctctt tcggtcaatc ggtgggttct tccggagggt ctggtagcgt
     4141 caacgttaat actgtgggaa caaactgcgc ctgggccgca aacagtgccg taagttggat
     4201 catcat
//
LOCUS       DASUEX010000112         2637 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1512560, whole genome shotgun sequence.
ACCESSION   DASUEX010000112 DASUEX010000000
VERSION     DASUEX010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2637
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1512560"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1070
                     /locus_tag="VFV61_01915"
     CDS             <1..1070
                     /locus_tag="VFV61_01915"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEX4899256.1"
                     /translation="AALRTFGPQHRFSTGLWTNGAFNKSAGIIYGNLYVSGRDPSFHY
                     EHALEIAQQLNQLGIRTITGDLIVAPGFTMNFNWSARSSGERLYDTLDSTLRPAEAAG
                     AWTFARTTNGTQATMQTVPSVAVMGDVYVNPVAPGAKLLLTHQSSKLTDILKVLLCYS
                     NNFMAERLGDSLGGPEAVERELVKSLGIPAEELKLATLSGLGTNRVSPRAMMTVFRAL
                     REELRRYRLSTTDIMPVAGIDPGTLEERFTDAAERGSVIAKTGTLIRTDGGASALVGQ
                     MKTATGEILLFVILNQRGNVSRFRDNQDFLVKQVQQTRGGPKAFDYKPLALTMRLSDT
                     RSTVAASPNEYEPVTANGNSP"
     gene            complement(1164..1589)
                     /locus_tag="VFV61_01920"
     CDS             complement(1164..1589)
                     /locus_tag="VFV61_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899257.1"
                     /translation="MAITPPRIGQVVPDLGSDCSATPMPAVDARKSQGFSVCSHHPVC
                     DSVTFSNDGGTQLPNSPLECCGSVSGIRNAFALYLEHRELASRKLATTSSQDRPARVW
                     CRTLHAKLGGKRLAAFELAGRILLSTGWHSTLRRDDAWI"
     gene            1582..2622
                     /locus_tag="VFV61_01925"
     CDS             1582..2622
                     /locus_tag="VFV61_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899258.1"
                     /translation="MAIKQHNAKMQMQRGERGFSLIQVVIAVAVMAIVTTLAVLGIAS
                     ARQRIRLTNSSRLLASYLEKTRVDSVRRHAMTAGQMSVITFLNNRSYRVSMDFDGNGT
                     METRDINLDEGVQIATNPLPDPIFFDWRGRLVGTSEKKVSITLQYGTDRNDQRSIDVT
                     RSGDVTIDSDVYLDDVPNVNVNVNSLTGVDSGSTLNGNNNAIPTPTPQPSPTPTPDVV
                     PTPTPVPTPTPTPFPTPSPYPSLSPTPTPLPTVTPTPTPTPVPTPTPCLVSVTPPSLS
                     IRKNGGTGTVGFSISPMGPVSFTTGPSNLQVTQGSGNTFTVTSVNTSRGDFTLVFSTP
                     CGSTSVVVSVTN"
BASE COUNT          620 a          774 c          707 g          536 t
ORIGIN      
        1 tggcagcctt gcgcaccttt ggaccgcaac atcgtttcag caccggtctg tggaccaacg
       61 gcgccttcaa caagtcggcc gggatcatct acggaaatct ctatgtgtct ggccgcgatc
      121 cctcgtttca ctatgagcat gcgctggaaa tcgcccagca attaaaccag ctcgggatca
      181 gaacgatcac tggtgacctg atcgttgcgc ctggtttcac gatgaacttc aactggtctg
      241 cccggagttc cggcgaacgt ctctatgaca ctctcgattc gacactgaga cctgctgaag
      301 cagccggcgc ctggactttt gcccggacaa cgaatgggac gcaagccacc atgcagacgg
      361 ttccgagcgt cgcagtcatg ggtgatgttt acgttaatcc ggttgcgcct ggggcaaagc
      421 ttctgctcac acaccagtca agcaagctta cagacatact caaggtgctg ctctgctact
      481 caaacaattt catggcagaa agacttgggg atagtttagg tgggccagaa gctgtggaac
      541 gcgagctggt taagtccctc ggcatcccgg cggaagagct caaacttgcc acgctcagcg
      601 ggttgggaac taatcgagtc agtccgcgcg caatgatgac agtcttccgc gctctgcggg
      661 aggaactcag aagatacagg ctctcaacga cggacataat gcccgtcgct ggaatcgatc
      721 cgggaacgct tgaggaacga ttcaccgatg cggcagagcg cggcagcgtg attgctaaaa
      781 cgggaaccct gatcagaacc gatggcggcg ctagcgcgct cgtgggccag atgaagaccg
      841 cgacgggcga aatccttctc ttcgtgatcc ttaatcagcg cgggaatgtg tcgcggtttc
      901 gcgacaatca agacttcctg gtgaaacagg tccagcaaac tcgcggaggg ccgaaagcgt
      961 ttgactacaa accactagcg ctgaccatga ggctctccga cactcgcagc acggttgcgg
     1021 cttcaccaaa cgaatatgag ccggtaacgg ccaatggaaa ctctccctga actcttgacc
     1081 acctgcaatc gacgactctc gtcataaaac ctgtcactcc gaaaggttct cactgatttc
     1141 tcactgctct ccgcctgcac aattcagatc caagcatcat cacgccgtag tgtggagtgc
     1201 cagccggtag acaaaagtat gcgccccgcc agctcgaaag ctgcgaggcg cttaccgccc
     1261 agcttcgcat gcagcgtgcg gcaccacaca cgggcaggac gatcctgaga acttgtggtc
     1321 gccagctttc tggaagccag ctccctgtgt tccagataaa gagcgaaagc gttacgaatt
     1381 ccggagaccg acccacagca ttccagcggg gaattcggaa gttgagttcc tccgtcgtta
     1441 ctgaaagtta cgctgtcgca gactggatga tgagaacaaa ccgagaaacc ctggcttttg
     1501 cgggcatcga ctgctggcat gggtgttgcc gagcaatcgc tgccgagatc cggcacgacc
     1561 tgaccgatgc gtggaggagt aatggccatc aaacagcaca acgcgaaaat gcaaatgcaa
     1621 cggggcgagc gtggcttcag cctaatccag gtcgtaatcg ccgtcgcggt gatggcaatc
     1681 gtgacgactc tggcggtact gggaattgcc tctgcgcgac aaagaatccg gcttacaaac
     1741 tcttcacgct tgctggccag ctatctcgag aagacacgag ttgattcggt tcgacgacac
     1801 gcgatgacgg cgggtcaaat gagcgtgatc acgtttctca acaatcggtc ttatcgcgtg
     1861 tcgatggact ttgatggcaa cggcaccatg gaaactcgcg acattaacct ggatgagggt
     1921 gttcagatcg ccaccaatcc gctgcctgat ccaatcttct tcgattggcg aggccggtta
     1981 gttggcacca gtgagaagaa ggtcagtatc acgctgcaat atggtaccga taggaacgat
     2041 caacgcagca tcgatgttac gcgatctggt gacgttacca tcgatagcga cgtttacctc
     2101 gatgacgtgc ctaatgtaaa tgtgaacgta aactcgctga caggggttga ttcaggctcc
     2161 actctaaacg gcaacaacaa tgcgatcccg acgcctactc cccagccgtc accaacgcca
     2221 acccccgacg tggtcccgac gccgactccg gtgccgacac cgacgccaac tccattccca
     2281 acgccaagcc cgtatccatc gttatcgccg acacctactc cgctgccaac agttacgcca
     2341 acaccgactc cgacgccggt tccgactcca acaccgtgcc ttgtgtcggt aacgccgccg
     2401 tctctgtcca ttcgtaagaa tggcggcact ggaacggtcg gtttcagcat ctcgcccatg
     2461 ggcccggtga gcttcacaac tggacccagc aatctgcaag taacccaggg ctccggcaat
     2521 acttttaccg tgacctccgt gaacacctca cgaggtgact ttacgttggt gttcagcaca
     2581 ccttgcggct ctacgagcgt cgtggtgagc gttacgaact gaggcgcaat tcagacg
//
LOCUS       DASUEX010000113         4023 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_710880, whole genome shotgun sequence.
ACCESSION   DASUEX010000113 DASUEX010000000
VERSION     DASUEX010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4023
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_710880"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            87..1256
                     /locus_tag="VFV61_01930"
     CDS             87..1256
                     /locus_tag="VFV61_01930"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HEX4899259.1"
                     /translation="MPVKIDDLLRVATSHGASDLHLKVGSFPVMRIGGELHPISDAPR
                     VKQEDALDMAFSMMSNRQKQRFKEASEVDIGYGVSGLGRFRANIFQQRGSVSIVLRVI
                     PEQTRSTTELGLPPVIDKIAEERRGLILVTGSTGSGKSTTLAAMIDRINAIRSGHIVT
                     IEDPIEFLHKDKMSFVTQREVDVDTRSFSEALRGALRQDPDVILVGEMRDYETIETAL
                     MAAETGHLVLSTLHTLDATETITRIVSCFPSHQQKSVRIQLAGILKGVISMRLVRAAT
                     GAGRVPAVEVMVSTGLIRDYIVNEEKTYLIREAIAAGTSQYGMQTFDQSLFNLFQSGL
                     ISLEEAVHNASNADEFKMRVSGILSAEQAINTMNPRVETISQSKDRNTGSLFVKH"
     gene            complement(1597..3135)
                     /locus_tag="VFV61_01935"
     CDS             complement(1597..3135)
                     /locus_tag="VFV61_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899260.1"
                     /translation="MFEALTDPARAAAAREQIHEYFRQHSEWFCTLGEGNVQALNRSE
                     LAIDLLNGRLIFSCWTEQGTRSWRVGAWKRSGEKLLLEASRRLGAERPVIEIIPRASA
                     SSIAATVKAARLLRCEQLARLAASSQPGLLKIERTSLSPGARRGQPGKYARIILRQKH
                     QRIAVTGSVAASKASETDAFLASALLWFARASERSRPPYIQQLWLIVDREIKKSLTAR
                     LALLRDSLRETIVVCELDDGWTGLAEVTCPDRKDLWKKRLSRFPPGAEPEVSELAAAI
                     TAEAPQAIDIVHARHGQTLRYLGMPFARVRRVFSSERVWFGIEGSHRRLLEEKTFHFY
                     AELMNDLRAHRAATAPDQRHALYRNAAEAWLESILRRDITQLDPGLIIAPLYAQFRLA
                     RGGRSGVRPIDLLALRQDGRLVVIELKVSEDREHVLQGADYWQRVEAYRRRGHIARAK
                     LFGERKISNEAPLVYLVAPTLRVHPAFQTLARTIASDIEIYRFDINEDWRSGVRVMRR
                     LRVN"
     gene            3399..>4023
                     /locus_tag="VFV61_01940"
     CDS             3399..>4023
                     /locus_tag="VFV61_01940"
                     /inference="COORDINATES: protein motif:HMM:NF012336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEX4899261.1"
                     /translation="MKNVYLAASVRTPIGRFGGTLASWTAADLGVAVAKETLRRAALQ
                     AGDIQESIWGCARQAGGGPNVGRQITYRAGAPETVPAFTVNQACGSGLKAIILAAQEI
                     LLDRANVVLAGGTESMSRVPYFAEGARWGLRLGDQQLVDGMYRDGFNDPLSGLVMGET
                     AENLARKYEIPRDEQDEFALRSQQRAQLAIESGRFDDEIVPLEIKGRR"
BASE COUNT          875 a         1138 c         1151 g          859 t
ORIGIN      
        1 gcgttcctgt ctcacccaaa aaattcagaa ctcttagcct taacatcagg atctgatcac
       61 gtcttgcgag gtattaaagt cagcccatgc ccgtaaaaat tgacgatctg cttcgagtgg
      121 ccaccagcca cggtgcctcg gatctccacc ttaaagtggg atcatttcct gttatgcgta
      181 tcggaggcga actgcacccc atcagcgacg cgcctcgcgt gaaacaggaa gatgcgctgg
      241 acatggcctt ttcgatgatg tcgaaccggc aaaaacagcg ctttaaagaa gcttcagaag
      301 tagacatcgg ttatggcgtc tccggcctgg gccgatttcg cgccaacatt tttcagcagc
      361 gcggcagcgt gagcatcgtg ctgcgcgtaa ttccggaaca aacgcgaagc accactgaac
      421 tcggactgcc tccggtaatc gacaagattg cggaagaacg acgaggactg attctggtaa
      481 cgggatctac aggttcggga aagtcgacga cgcttgccgc catgatcgat cgtattaacg
      541 cgatccgcag tggacatatt gtaacgatcg aagatcccat cgagtttttg cacaaggaca
      601 agatgtcgtt tgttacgcag cgagaagttg acgttgatac gcgatcgttc tcggaagctt
      661 tgcgcggcgc cctgcggcag gatccggatg tgattctggt cggcgaaatg cgcgactacg
      721 aaacgattga gacagccttg atggcagccg agacgggtca cctggtgctc tccacactgc
      781 acacgctgga cgcgacggaa accatcacgc gcatcgtttc ctgttttcct tcacaccaac
      841 agaagtctgt gcggattcaa ctcgcaggaa tacttaaagg tgtgatttca atgcgactcg
      901 tgcgcgccgc cacaggcgcg gggcgcgtgc cggctgtgga agtgatggtt tcgacgggac
      961 tgattcgcga ctatatcgta aacgaagaga agacctactt gattcgtgaa gcgattgcgg
     1021 ccggcacctc gcagtacggc atgcagactt tcgatcagtc tctctttaat ctgtttcagt
     1081 ccggcttgat ttctctggaa gaggccgtgc acaacgcgag taacgcggac gagtttaaga
     1141 tgcgcgtctc cggcatcctt tcggctgagc aggcgatcaa tactatgaat ccgcgagtag
     1201 aaacaatctc gcagtcgaaa gatcgaaata cgggatcatt gtttgtgaag cactgaactt
     1261 tttggccttg cctcctaagt cgccctcgac gtaggttacg gctttgccgc cggatgtgag
     1321 gcgcggctcg ccgcgccgag agcggccaga gtattcgcgg gctatgcccg ctagttcgcg
     1381 ctcaagcctt aaacctaatt ggccaaagca cgcaccacgg gcatagccca agcatgtaat
     1441 gggcacctct ccggcggggc atagccacca cctcaaatct ggtggcacag ccgaaacgga
     1501 gatggcccat gactttcgag gcaacgcaga tttcttttcg gagtggcgca acctcctggc
     1561 ttgcgctcgc aaactgacag ttttgcgtac gagagctcag ttcactcgca agcgccgcat
     1621 cacgcgtact ccggaacgcc agtcttcatt aatgtcaaaa cggtagatct cgatgtcgct
     1681 cgcgatagtg cgcgcaagcg tctgaaaagc cggatgcacc ctgagcgtcg gagcgacgag
     1741 atagacgagc ggcgcttcgt tgctgatttt gcgctcgcca aacaacttgg cccgcgcaat
     1801 gtggccgcga cggcggtagg cttcaacgcg ttgccaataa tccgctccct gcaacacatg
     1861 ctcacgatcc tcagagactt tcagttcgat gacaaccagg cgcccatcct gccgcagagc
     1921 cagcaagtca atcggacgca cccctgaacg accaccccgc gccaaacgaa actgagcata
     1981 aaggggcgcg atgatcagac ccggatccaa ctgggtgatg tcgcgtcgca ggatcgattc
     2041 gagccaggcc tcggcggcgt ttcgataaag cgcatgccgc tgatccggcg ccgtcgccgc
     2101 tcgatgcgcg cgcaggtcgt tcatgagctc ggcatagaaa tggaaagtct tttcttccaa
     2161 cagccgtcga tgcgatcctt ctatcccaaa ccaaactcgt tcgcttgaga acacgcgacg
     2221 cacccgcgcg aagggcattc ccaggtaacg cagggtttgc ccatggcgcg catggacgat
     2281 gtcgatcgcc tgcggagctt ctgcagtaat cgcggctgct agttcgctga cttcgggttc
     2341 cgccccgggg ggaaatctcg aaagcctttt cttccaaaga tctttgcggt cgggacaagt
     2401 aacttccgcc aacccggtcc agccgtcgtc aagctcgcag accacaatcg tttcgcggag
     2461 actgtcgcgc aacaaagcca gccgtgctgt taacgacttt ttgatttcac gatccacgat
     2521 cagccacagt tgctggatgt aaggggggcg ggaacgttcg cttgcgcgag cgaaccaaag
     2581 cagcgctgaa gccagaaaag cgtcagtctc gctggctttg gaagcagcca ccgatccggt
     2641 gacggcaata cgttggtgct tttgacgcag gattatgcgc gcgtacttgc ctggctgccc
     2701 gcgcctggct cccggactga gggaggtccg ctcgatcttt aataaacccg gttggctgga
     2761 agcggcgagc cgcgccagtt gttcgcagcg cagcaggcgc gctgccttaa cggttgcagc
     2821 gatcgaactc gccgaagctc gcggaatgat ttcgattacc ggtcgctccg caccgaggcg
     2881 gcgagaagct tcgagtagca gcttttctcc ggaccgcttc caggcgccaa cgcgccagga
     2941 gcgtgttcct tgttcagtcc aacaggaaaa aatcaaacgc ccattcagca ggtcaattgc
     3001 caactcactg cgattcagtg cttggacgtt tccctcacca agcgtgcaga accactcgct
     3061 gtgttgacgg aagtattcgt ggatctgttc gcgggcagcg gcggcgcggg ccggatcggt
     3121 gagggcttcg aacatgttgg tgaggttctt acatgaaggc ggctcaaatc tcgcttaaca
     3181 ccgccaaggc tgccaagtta cgccacggtc gccaaggatt tatggcgaag aactgatagc
     3241 ccataccacc ttggcgctct cggcgtttct tggcgccttg gcggtttgat tctggtgttt
     3301 gttcgccatc gcttcactgt cgttgttttc gtcgttactt ctaccgtgtg ctaccatttc
     3361 cggtcaacta attccgaaaa cttctatatc caagagtaat gaaaaacgtt tatcttgccg
     3421 catccgtgcg cactccgatc ggacgatttg gcggcaccct cgcttcctgg accgcggccg
     3481 atttgggtgt tgccgttgcg aaggaaacat tgcgacgcgc ggccctgcaa gccggtgaca
     3541 ttcaggaatc gatttggggt tgtgcgcgcc aggcgggtgg cggaccgaat gtcggacgcc
     3601 aaatcaccta tcgcgcgggc gcgccggaaa ccgtgccggc attcactgta aaccaggcat
     3661 gcggatcagg attgaaggca attattcttg cggcccagga gattctgctc gaccgcgcca
     3721 atgtggtgtt ggctggtggt acggagagca tgtcgcgcgt gccctacttc gctgaaggag
     3781 cgcgctgggg cctgcgcctc ggcgatcagc agctcgttga cggcatgtat cgcgatgggt
     3841 tcaacgatcc gctttccgga ctagtgatgg gagagaccgc agagaacctc gcgcgcaaat
     3901 atgaaatacc acgcgacgag caggacgagt ttgccttacg ttcgcaacaa cgagcgcagt
     3961 tagccatcga gtcgggtcgt tttgacgacg agatcgtgcc cttggaaatc aaagggcgga
     4021 gag
//
LOCUS       DASUEX010000114         3829 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1512617, whole genome shotgun sequence.
ACCESSION   DASUEX010000114 DASUEX010000000
VERSION     DASUEX010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3829
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1512617"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="VFV61_01945"
     CDS             <1..251
                     /locus_tag="VFV61_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899262.1"
                     /translation="SLLPTSCRHHPRTLVQSGTNSELYQHLAQEQKAYCCLGLLPSLS
                     EVMGGGMSKKGFSSLACLKVVTAALERPVAEPIFAYER"
     gene            complement(349..>3829)
                     /locus_tag="VFV61_01950"
     CDS             complement(349..>3829)
                     /locus_tag="VFV61_01950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899263.1"
                     /translation="GSNLNAGSRLRFNGVDHAVTFISTTEVHAQIAANEIAVPGIVPV
                     EILNPAPGGGISLTQNFTIINTGPTVSSLAPANAVAGGPAFTLTVNGTNFDATSVVLW
                     NGVSRTTTLVSSTQLTAQITAADIQSAGSFAVTVSNSGGTPSNALNFIVNNPLPQITA
                     ITPTSVSSGGPSFTLTVNGTGFVPGSVVRFNGNLAANDRPTVFDNNTQLTATITAADI
                     AAPGAFPITVFTPLPGGGVSNAINLTVENPAPIITSLTPASMTAGAADFTLSINGSNF
                     NNNSVVRVDGIDSATTFVSSSALSVQILAAKVASPGAISIGVFNPGAGISNSIQLQVS
                     NPQPVISNISPVSKTAGDSAFTLTVNGTGFNGTSVARLSGNDRPTTFVSSSQLDVQLS
                     AADLSSAGLYPITVFNPAPGGGTSSAINLTVNNPVPVIANLAPSSLLQGATTTQVTVN
                     GTGFVSGSVVAWNGQSRSTTFVSPTQLNFQVTDSDLNVEGVASVTVNNPAPGGGNSAP
                     SPFSVNMNPRRVSVVSSSGFSGGSASVTIQLTAQGDENALGFTLFYDTAILSNPTVSL
                     GADSADASLNINTSEVGAGHYGVALSLPAGMTFSAGARQVVIVNFDVAAVPSGTQTQI
                     TFGDQPITREVANITAEVLPTAFVPGSITVSDGVEADVAPRPNGSGNGMVTVSDWVQI
                     GRFVAGIDTVASSGEMQRADCAPRSSLGNGALTISDWVQAGRYAAALDTVLPAGGPMT
                     IPSGVFEAKDSGDMHANSVREPSINVPRSVRMTVLQEQGQERSIAIELDSYGTENGLG
                     FSLMFDPSKLRFRSAENGANAESATLQINQASADKGRLGVALVLPVGHSFNAGTNQIV
                     ILKFIELQPEGQPSGVTFTDLPIKRELVSVDAGNLVAGFDGLPEVNSLDNAQYFVYRQ
                     YLDLLGRLPDSEGLDYWTEQISSCGTDEACVRQRRVAVSNAFLLGREFQQTSGFILRM
                     YRAGLGIRPTYAQFIKERSSLMTGMQIENGKTEFANYLVQLPEFELLFPRTTTPTQYV
                     DGLNANTGNALTPSQRAAFIDGLQSGNESRASVLRQIADNPVFADREFNSSFVLMQYF
                     GYLKRDPDAEGFAFWLDQLVPFGSRARETQRAMVWSFITSSDYQMRFSSAGGDCRRSG
                     QNRDCLVEPGIER"
BASE COUNT          925 a          997 c         1040 g          867 t
ORIGIN      
        1 ccagtttact tcccacgtct tgccgccatc atccgagaac gcttgttcaa agcggcacga
       61 attcggagtt atatcagcat cttgcgcagg agcagaaagc atattgttgt ttgggattac
      121 tgccgagcct gtctgaggtg atgggcggcg gtatgagcaa gaaagggttc agcagtcttg
      181 cgtgcttaaa agttgtaacc gccgccctgg agaggcctgt tgccgagcct atcttcgctt
      241 acgaaagatg aacggaagat aaattcgagc gaacaggata atgaaaatat ggggatttgc
      301 tgaatttcac cagtattcgt tctattcaaa tggtcctatt tccccaggtc aacgctcaat
      361 cccgggctca acgagacagt cccgattttg accggaacgc cggcaatcac cgccagcgga
      421 actgaagcgc atttgatagt cagatgaggt tatgaaggac caaaccatcg ctcgttgggt
      481 ctcacgagca cgtgatccga aggggactaa ctggtcaagc cagaatgcga agccttccgc
      541 atccggatcg cgctttagat aaccaaagta ctgcatcagc acaaaagagg agttgaattc
      601 ccgatcagca aatacagggt tgtcggcaat ctggcgtaga actgaggcac gcgattcgtt
      661 gccgctctgc aaaccatcaa tgaatgctgc cctttgcgat ggtgtcaagg cgttgccggt
      721 attcgcgttg aggccatcaa catattgagt gggtgtagtc gttcgcggaa agagcagttc
      781 gaattctggc agttggacca gatagtttgc gaactcggtc ttaccgttct cgatttgcat
      841 tcccgtcatc aagctgcttc gttcttttat gaactgcgca taagttggtc taatcccgag
      901 cccagctctg tacatgcgaa gaatgaaccc actagtttgc tgaaattccc gcccgagcag
      961 aaaagcattt gagacggcca cacgtcgctg ccgcacacaa gcctcatccg ttccacaaga
     1021 gctgatctgt tccgtccagt agtcaagacc ctctgagtca ggcaaccgcc caagcagatc
     1081 gagatactgt cgatagacga aatactgtgc gttatcgagt gaattcacct ctgggagccc
     1141 atcaaagccg gccaccaaat tgccagcgtc gacggaaact agttcacgtt tgattggcaa
     1201 gtcagtgaag gtaactccgc ttggttggcc ctcgggctgg agttcaataa atttgagaat
     1261 gacaatctga tttgttcctg cattgaaact atggccaacc ggtagcacca gcgcgacgcc
     1321 aagtcttccc ttatcggcgc tggcctggtt gatctgcaag gttgcggact ctgcattcgc
     1381 gccgttttcg gctgaccgga atcgcagctt cgatggatcg aacatcaaac taaagccgag
     1441 tccgttttcg gttccgtaag agtcaagttc gatcgcgata cttctttctt gtccttgttc
     1501 ctgcagcact gtcatgcgga cagaccgcgg aacgttaatg ctcggctctc taacagaatt
     1561 tgcatgcatg tcgccggaat ctttcgcttc aaagacacca ctcggaatag tcattggtcc
     1621 acctgctggg aggaccgtgt ccaaggcggc tgcataacga cctgcttgaa cccaatctga
     1681 aattgtgagt gcgccgttgc ccaggctgct tctgggagcg caatcggcac gctgcatttc
     1741 accgctagaa gcgacggtgt cgatacccgc gacaaaacgc ccaatttgta cccaatcaga
     1801 aacggtgacc ataccgttgc cggaaccgtt cgggcgcggc gcaacatcag cttcgacccc
     1861 atccgacact gttatcgacc ccggaacaaa cgcggttggc aacacctctg cagtgatatt
     1921 ggcaacttcg cgagtaatcg gctgatcgcc gaatgtgatc tgcgtttgtg ttcccgaggg
     1981 aacagcagca acatcaaagt taacaatcac cacctgtcgg gcaccggcac tgaaggtcat
     2041 tccagccggc aaggacaatg cgacgccata atgtcccgcg cctacctcac tggtgttgat
     2101 attcagcgac gcgtcggctg aatccgcacc aagactaacg gtgggattac tcaagatcgc
     2161 agtgtcgtag aaaagcgtaa agcctagcgc gttctcatct ccttgtgccg tcaactgaat
     2221 tgttacactt gcacttccgc cggagaaccc ggacgaacta acaacactta cacgcctggg
     2281 attcatgttg acggagaagg ggctcggagc cgagttgccg ccgcccggag caggattgtt
     2341 caccgtcacg ctggccactc cctcaacgtt gagatccgaa tccgtgacct gaaaattaag
     2401 ttgtgtcggg ctcacaaacg tggtcgagcg actttgacca ttccacgcga caacggaacc
     2461 gctaacaaag ccagtgccgt tgactgtcac ttgggtggtg gtcgcacctt gcagcaaact
     2521 ggaaggcgcc aggttcgcta tgaccggtac agggttgttg acggtcaagt tgatcgcgct
     2581 tgaagtccca ccacccggcg caggattgaa aacagtgatt ggatagagac ctgctgaaga
     2641 gagatcagcc gcactgagtt gcacatcaag ttgcgacgag ctgacaaagg tggtgggacg
     2701 atcgtttcca ctgagccgag cgaccgatgt gccattaaag ccggtaccat taacagtcag
     2761 cgtgaacgct gagtctcctg ctgtcttgct gacaggcgag atattcgaga tcactggctg
     2821 cgggttggag acctgcaatt gaattgaatt cgagattcca gcgcctggat tgaaaacccc
     2881 aatactaatt gctccggggc tcgccacctt tgcagcaagt atctggacgc tcaaagcaga
     2941 gctgctgaca aaggtcgttg cagaatcgat cccgtcaacc cgtaccactg agttgttatt
     3001 gaagttagag ccgttgatgg agagtgtgaa atcagccgcc cccgctgtca tgctcgcggg
     3061 cgtgagactg gtaatgatag gcgcagggtt ctccacagtt agattaattg cgtttgaaac
     3121 cccgccacca ggaagcggag tgaatacggt tatcgggaag gcgccaggag cagcgatatc
     3181 ggcagcagta atagtcgctg ttaattgagt gttgttgtca aagactgttg gtctatcgtt
     3241 cgcagcgaga ttgccattga agcggacaac ggagcctggc acgaatccgg taccgttaac
     3301 tgtgagcgtg aaagaaggac cgccgcttga aacggaggtc ggggtgatcg ccgtaatttg
     3361 aggcagcggg ttattaacaa tgaagttaag cgcattcgaa ggcgtgccac cagaattcga
     3421 aacggttacg gcaaacgatc ctgcgctttg aatgtccgcg gccgtgattt gcgcagtcaa
     3481 ctgcgttgag ctaaccaagg tcgtggtgcg actcacgcca ttccagagca caactgaagt
     3541 tgcatcgaag tttgtgccgt tcacggtcag ggtaaatgct ggcccaccgg ccacagcgtt
     3601 tgctggagcc aaactggaga cggttggtcc cgtattgatg attgtgaaat tttgcgtcag
     3661 agagatacca ccgccaggcg caggattaag gatctccacc gggacaatgc cggggacggc
     3721 aatctcgttt gctgcaattt gggcgtgcac ttcggtggtg ctaataaagg tcaccgcgtg
     3781 gtccacacca ttaaaacgga gccggctgcc ggcattcagg ttgctgcct
//
LOCUS       DASUEX010000115         2408 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_825823, whole genome shotgun sequence.
ACCESSION   DASUEX010000115 DASUEX010000000
VERSION     DASUEX010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_825823"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1079)
                     /locus_tag="VFV61_01955"
     CDS             complement(<1..1079)
                     /locus_tag="VFV61_01955"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX4899264.1"
                     /translation="MDETIKPGSEADTWEKTVRDPLATTRTPLEAIIGTTLSGRYRIQ
                     RKLGHGGFGIVYLASDEKMLSRPVVVKALADDGNENDWIAKKFKQEIEALARIDHPSI
                     VGIFDVGEMPDGKPYIVMQYVDGFSLRSLITPEGLDFWRAANIMRQTGRALSAAHDRG
                     ILHRDLKPENIMVQTLGGGDEQVKIIDFGIARVKNSVVSFTTASDKTVGTIAYMSPEQ
                     LIAAKLTPASDVYSLAVIAYELLTGRRPNNPESGYQLREMQEAGVRIKPTDLRPGLPL
                     EAEAIILKGLSFKAESRPQRARDFGDFLAAALTEDDEHTVLASAAIPLPSPQDVIGPE
                     APAPLQSSVSEQTTTAPSASRLRRWG"
     gene            complement(1105..>2408)
                     /locus_tag="VFV61_01960"
     CDS             complement(1105..>2408)
                     /locus_tag="VFV61_01960"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HEX4899265.1"
                     /translation="KNPQAKEDIISKDRRQPKVTIAAAEMTPADGRQRERMLRLKLEV
                     AEAPAQAEYREGSGAQDLRLFRNGLLVRTWSGDLLEGGANKTIETSVPIVAGENRFTA
                     YAFNRDNVKSTDDVLSVSGAESLRRSGTAYLIVAGVGNYANPQYNLNYSVADATAIGQ
                     QLKQQQELLARYNPIVVVSLLNEEATRGNILLALKLLAGEGGGSLPANAPRDLSKIKP
                     AQPEDAVIFYFSGHGTAVEDRFYLIPHDLGYMGPRSRLNREGLEAIISHSISDLELEA
                     ALRPVDVDQLLLVIDACNSGQALNAEEERRGPMNAKGLAQLAYEKGMYVLTASQSDEV
                     AFESEGLKHSYLAYALVEEGIKSSAADADRNGQVLLKEWFDYATDRVPQIGKQKVVAG
                     KQLEEVDPDERRVQRPRVFNMREGGAERFVIAGVARAGLNQ"
BASE COUNT          476 a          712 c          644 g          576 t
ORIGIN      
        1 ccccatcgcc tcagccttga ggccgaaggg gcagtagtgg tttgttccga tactgaactt
       61 tgaagaggcg ctggcgcctc aggcccgatc acatcctgag gcgacggtaa tgggattgcg
      121 gcactcgcca ggactgtatg ctcatcgtct tcagtaagcg cggcggcgag aaagtccccg
      181 aagtcgcgag ccctttgggg acgcgattcc gctttgaacg aaagtccctt caggatgatt
      241 gcctctgcct caagcggaag accgggacgc aaatcagtgg gcttgattcg cacaccagcc
      301 tcctgcatct cgcgtagttg gtaacccgac tccggattat ttggacgtct cccagtcagc
      361 aactcgtaag cgatcaccgc caaactgtag acatcgctgg ccggagttag ctttgccgca
      421 atcaattgtt ccggagacat gtaggcaatg gtgcccaccg tcttatcgct tgcggtcgta
      481 aacgaaacga cagagttttt aacgcgagca atgccgaaat cgatgatctt cacctgctca
      541 tcgccgccac ccaacgtctg caccatgatg ttttcgggtt tcagatcccg atgcaggatc
      601 ccgcggtcgt gcgcggcgct gagcgcgcga cccgtctgac gcatgatgtt ggcggcgcgc
      661 caaaaatcca ggccttcggg cgtgatcagc gaccgcaggc tgaaaccatc gacatactgc
      721 atgacaatgt aaggcttgcc atccggcatt tcaccgacgt cgaagatgcc gacgatgctg
      781 ggatgatcaa tccgagcgag ggcttcgatc tcctgcttaa actttttcgc aatccagtcg
      841 ttctcgttgc cgtcatccgc gagggctttg accaccactg gccgggataa catcttttcg
      901 tcggaagcca gatagacgat tccaaatcca ccgtggccca gtttgcgctg aatgcggtaa
      961 cgtccgctga gcgtggtgcc gatgatggct tcgagcggag ttcgtgtggt cgcaagagga
     1021 tcacgaacgg tcttttccca ggtgtctgcc tcagatcccg gtttgatggt ttcgtccatt
     1081 ggcactggcg aatttccctt gcgcttactg attcagtccg gcacgggcga cgccggcaat
     1141 gacaaacctc tccgcgccac cttcgcgcat attgaaaacc ctgggtcgct gcacgcggcg
     1201 ctcgtcagga tcaacttctt ccaattgctt tcccgcgact accttttgtt ttccgatttg
     1261 cggaacgcga tcagtcgcgt agtcgaacca ttcctttaac agcacctggc cgttccggtc
     1321 ggcgtcggct gcgctggatt ttattccctc ttcaaccagc gcgtaagcta aataactgtg
     1381 tttgagcccc tccgactcaa aagccacttc gtcactctgt gaagcggtta agacgtacat
     1441 ccctttttca taggcgagct gggctagtcc ttttgcattc atcggcccgc ggcgctcttc
     1501 ttccgcgttc agtgcctgac ccgaattgca ggcgtcgatc acgagtagca gttgatcaac
     1561 atcgactggc ctgagcgcgg cctcaagctc aagatcggaa atactgtggc tgatgattgc
     1621 ctcgaggccc tcccgattta aacgagacct cggccccata tatcccaggt catgtgggat
     1681 caggtaaaag cgatcttcca cggcggtgcc gtgtccggag aaatagaaaa ttacggcgtc
     1741 ttcgggttgc gctggtttta ttttcgagag atctcgcggc gcgttcgccg gcaatgaccc
     1801 accaccttcg cccgcaagca gcttaagcgc cagcaggata ttcccccgcg ttgcctcttc
     1861 attgagaagt gaaaccacga ctatcgggtt atagcgcgcc agcaattcct gctgttgttt
     1921 aagctgctgc ccaatcgcag tggcgtccgc gacgctgtag ttcaggttgt actgcggatt
     1981 ggcgtagttg cccactccgg ccacgatcag gtaagccgtg ccggaacgcc ttagcgattc
     2041 agcgccgctt acggaaagca catcatctgt cgacttaaca ttgtcgcgat tgaacgcgta
     2101 ggccgtgaag cgattttcgc cagcgacaat tggtacgctc gtctcaatag ttttgtttgc
     2161 gccgccctct aacaaatctc cggaccaggt ccggaccagc aagccattgc gaaacagtct
     2221 caaatcctgg gcaccacttc cctcgcggta ctctgcttgg gccggagctt ctgcaacctc
     2281 tagtttaagg cgcagcatcc gctctcgttg cctgccatca gctggggtca tctcggcggc
     2341 cgcgatagtc actttcggtt ggcggcgatc cttgctgata atgtcttcct ttgcctgcgg
     2401 gttcttgt
//
LOCUS       DASUEX010000116         3831 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_240492, whole genome shotgun sequence.
ACCESSION   DASUEX010000116 DASUEX010000000
VERSION     DASUEX010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3831
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240492"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1006)
                     /locus_tag="VFV61_01965"
     CDS             complement(<1..1006)
                     /locus_tag="VFV61_01965"
                     /inference="COORDINATES: protein motif:HMM:NF024884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HEX4899266.1"
                     /translation="MGYIVAVIVIAAATIVLRVFGEHINPTTVALAFLLIVVFLATAW
                     GPKPAIVASLLGVACFNFFFLPPLHTFTIADPENWIALFAFLITAITVGQLSAHAKRR
                     AEEANTARRESEALYNELQDTFEQASQTKALKQSERLKSALLDAVTHDLRTPLASIKA
                     SVTTLLDEQSASADHDAESRLDEAERQEMLEVIDEECDRLNRFIGGLIELARIEAGEM
                     HLRRRWGAVDEIISTALARAEPLTHGRKVTLDIEAELPVVRVDEQAVSEVVYTLIDNA
                     VKYSPPEMPIEIAAKRSGEGYVEMFVEDRGTGIPPHLRQRVFDKFFRATRDGNVSTNQ
                     P"
     gene            complement(1060..2928)
                     /locus_tag="VFV61_01970"
     CDS             complement(1060..2928)
                     /locus_tag="VFV61_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899267.1"
                     /translation="MCLTGVDYFSTLGYQPGIAFLAAGALSPIATLVLVLLTLFGALP
                     IYNRVAAESPHGEGSISMLEHLLSRWKGKLFVLVLLGFVATDFIITITLSAADATAHI
                     IENPFTPALFRHPVGVTLVLIIFLAAIFLKGFKEAIGLAVVLVTAYLLLNVIVIGSGL
                     YELFTHPVHFSQWKDAVLAYPQVHGNVWLVLLVSLIIFPKLALGLSGFETGVAVMPLV
                     KGDRELTKEQLEEIQSTSTGSPKPDAAAILEGRIRNTRKLLRTAALIMSVMLITSSFV
                     TAALIPAEKFAEGGEANGRAISYLAHESFGDIFGTVYDLSTIFILWFAGASAMAGLLN
                     IVPRYLPRFGMAPDWARATRPLVLVFTIIAVAVTLIFKADVDAQGGAYATGVLVLMSS
                     AAIAVTLAMYRRRRHWHSFFAISLVFAYTTIQNIHERPEGIKIASFFILTIVLTSLIS
                     RVWRSTELRVERVELDDAARDFVRVAARGTVRIIANRRDQGDAKEYETKEYEKRTDNH
                     IPPGESVLFFEVTPGDASEFSGVLKIYGERVDGFRVLRAVSPAVPNAIAAFLLHLRDE
                     TGKLPHVYFGWSEGNPLSYLMKYIAFGEGDTAPVTHEVLRQAEKDPERRPIVHVGG"
     gene            complement(3402..3620)
                     /locus_tag="VFV61_01975"
     CDS             complement(3402..3620)
                     /locus_tag="VFV61_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899268.1"
                     /translation="MATKHLRGRNKRLLTYLWVAVLAIVTIVLIYKEQTAVLYILATL
                     GVTAILLIVAFADLSRSAVGAGDSTPAK"
     gene            complement(3632..>3831)
                     /locus_tag="VFV61_01980"
     CDS             complement(3632..>3831)
                     /locus_tag="VFV61_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899269.1"
                     /translation="RVDYAAIKKNKQPDILLQAYDIVEIPEAGMFSPGRIGQTLLGTI
                     TGGLSSAMQSTGQIIPTRILY"
BASE COUNT          930 a         1071 c          979 g          851 t
ORIGIN      
        1 aaggttggtt cgtggacaca tttccatcac gcgttgcgcg aaaaaactta tcgaaaacgc
       61 gctggcgcaa gtgaggagga atcccggtgc ctcgatcctc gacaaacatt tctacgtaac
      121 cttcgccgct gcgctttgcg gcaatttcga taggcatctc aggaggtgaa tacttaactg
      181 cgttatcaat aagcgtgtac accacttcgc tgaccgcctg ctcgtccaca cgcaccaccg
      241 gcaattccgc ttcgatgtcg agcgtaactt ttcgaccgtg agtaagcggt tctgcacgtg
      301 ccagggctgt ggagataatt tcgtcaaccg ctccccagcg ccgtcgcagg tgcatctctc
      361 ccgcttcgat gcgcgccagc tcgattaacc ccccaatgaa acggttcagg cggtcacact
      421 cctcgtcgat gacctccaac atttcctgcc gctccgcttc gtctaatcga ctttcggcgt
      481 catgatccgc agacgcagac tgctcgtcca gcaacgtagt tactgatgct ttaattgagg
      541 ccaagggagt cctcaggtca tgagttaccg catcgagcaa agcagatttt agtcgctcgc
      601 tctgcttcag ggcctttgtt tgacttgctt gttcgaaagt atcttgcagc tcgttgtaga
      661 gggcctcaga ctctctccgc gctgtgttag cctcctctgc tcgccgctta gcgtgtgctg
      721 aaagctgccc cactgttatg gcggtgatca agaaggcaaa gagtgcgatc caattttccg
      781 ggtcggcaat agtgaaagta tgaaggggtg gcaggaagaa gaagttgaaa caggcaaccc
      841 ctaacagaga tgccacaata gcgggtttag ggccccacgc tgtagctaga aagactacga
      901 tcagcaagaa ggccagcgcg acagtagtgg gattaatatg ctctccgaag acccggagaa
      961 caatggtggc ggccgcgata acgatcacgg ctacgatgta tcccaagaaa tttttacgga
     1021 ccacaactct cctcaaacca cagttgtggg tctgtgcgtt tagccaccaa cgtgaacgat
     1081 cggccgccgc tctggatctt tctcggcctg gcgcagcact tcgtgcgtca cgggcgcggt
     1141 atcgccctcg ccgaacgcga tgtacttcat caggtaactg agcggatttc cctcgctcca
     1201 accaaagtaa acgtgcggca gttttccggt ctcgtcgcgc aagtgaagca ggaaagcggc
     1261 aattgcgttc ggcacggctg gagataccgc gcgcagcact cggaacccgt ctactctctc
     1321 tccgtatatt ttcaaaaccc ctgagaattc agacgcgtcg cccggcgtta cttcgaaaaa
     1381 cagaaccgat tcacccggcg gaatatggtt gtcggtgcgc ttttcatact cttttgtttc
     1441 gtactccttc gcgtctccct ggtctcggcg attggcaatg attcgcactg tgcccctcgc
     1501 cgcaacgcga acaaagtcgc gtgcggcatc gtcaagctcc actcgctcta cccgcaattc
     1561 ggtactacgc caaacccggg atataagtga cgtcaacacg atcgttagaa taaagaagga
     1621 cgcgatcttg ataccttcag gtcgttcgtg gatattctga atggtcgtgt aagcaaacac
     1681 tagtgagatg gcaaaaaagg aatgccaatg ccgacgccgg cgatacatgg ccagcgtgac
     1741 cgcgatggcg gctgaactca ttagcaccaa cactcccgta gcgtaggcac ctccctgggc
     1801 atctacgtca gccttgaaaa tcaaagtgac cgccactgcg ataatggtga acacgaggac
     1861 gaggggacgg gttgcgcgcg cccagtcagg ggccattcca aagcgaggca gatagcgtgg
     1921 cacaatgttc agtaaacccg ccatcgccga ggcaccggca aaccacaaga tgaaaatggt
     1981 gctcaagtca tagacagttc caaagatgtc gccaaacgat tcatgtgcca gatatgaaat
     2041 tgcacggccg ttggcctcac ctccttcagc aaacttttcg gcaggaatga gtgcagccgt
     2101 aacgaagctg ctcgtgatta acatcacgct cattatcagc gcggcagtcc gcaacagctt
     2161 gcgagtgttt cttattcgac cttcgaggat cgctgctgcg tcaggtttag gacttccagt
     2221 gctcgttgac tgaatctctt ccaactgctc tttcgttagt tctctatcac ccttcaccaa
     2281 cggcatgacc gcaacgccgg tttcaaaccc tgacaacccc agcgccaact tcgggaaaat
     2341 tatcagtgac accaatagga caagccatac attgccgtgc acctgagggt aggccagcac
     2401 cgcatccttc cattgcgaaa aatgcaccgg atgcgtgaac agttcataca gtccggaacc
     2461 aatgacaatg acattcagaa gcagataggc agtgaccagc accaccgcca atccgatcgc
     2521 ctctttaaac cccttcagga agatggcggc cagaaagatg atcaagacga gcgtcactcc
     2581 taccgggtgt ctgaagagag ctggagtaaa cggattttcg attatgtgtg ccgtggcgtc
     2641 tgcggccgat agcgtgatgg tgataatgaa atcggtggcc acgaacccca gcaaaacgag
     2701 gacaaacaac ttgcccttcc agcgcgacaa cagatgttcc agcatcgaga tcgagccttc
     2761 gccgtgagga ctttcggcgg ccactcgatt gtagattggc aaggcaccaa ataacgtcag
     2821 gagaacgagg acgagggttg ctatcggtga gagcgcgccc gcggcgagga aagcaatgcc
     2881 gggctggtaa ccgagagtcg agaagtagtc gacgccggtt aagcacatca cctgccacca
     2941 gggatgttgt ttgggacctg acccgcctgg tccccgatgg gattctgttt ccctcccgcc
     3001 cttaaacaac cagagcttga acttgcgcca tgacctctca gaaacaagca tcttgcggac
     3061 tagccaacgg gtctagtagt gcttggcctt ctcccttgtt gaatgtcgtc gaatcctact
     3121 cttattcgat cgcacgtcaa gaatctgctt tgcgctctac aaaaagtcct gggaaaacaa
     3181 aaaagcgggt gggggcctcc cacaaccccc accctaaggc cacgtgtcct caccccacgc
     3241 agcacttcgt ccaccgggga ggtgaacgca ggcggattat gcaacagtcc gcctaaactt
     3301 tcaataatgt gaaatggctt aaaacgggga aatctggcta gaattcgcct aatctggcaa
     3361 gcagcttttt tgcgatggat ctggagctaa acggatcgag attacttcgc tggagtcgag
     3421 tctccggcgc caactgcact ccgtgagaga tctgcgaacg caacgatcaa gagaatggcg
     3481 gtgacgccca gagtagcaag gatataaagc acggccgtct gctctttgta aatcaatacg
     3541 attgttacaa tcgcgagcac cgcgacccaa agataggtaa gcagtctttt gttacgtccg
     3601 cgtaggtgtt ttgtagccat tattaatcct gctaataaag aattcttgtc gggataattt
     3661 gtccggtcga ctgcatggca ctgctgagtc ccccggttat cgttcccagc aacgtctgcc
     3721 ctatacgtcc tggcgagaac atgcctgctt caggtatttc aacgatgtcg tacgcctgca
     3781 ataagatatc cggctgctta tttttcttaa tcgccgcgta gtcaactcga a
//
LOCUS       DASUEX010000117         3623 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_711520, whole genome shotgun sequence.
ACCESSION   DASUEX010000117 DASUEX010000000
VERSION     DASUEX010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3623
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_711520"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..762
                     /locus_tag="VFV61_01985"
     CDS             <1..762
                     /locus_tag="VFV61_01985"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX4899270.1"
                     /translation="KANGRISYVFEKAQGRSPALNAGVAATHGDLVGFIDDDEEVDES
                     WYEVVLAAFANEDVDFIGGPYVPRFESEVPKWLPKDHNAVVGIVDGGDKVVSFDDSYP
                     GMLMGGNAVFRREVLQRVGPYSTALGRSGTRLLSCEDEDMYQRLREAGARGLYLPQLI
                     VYHFIPSERLTKSYHRSWCFWQSVSSAVLDRIRPKPVAYVLGVPRYFYGRALRGMLRL
                     ANLREREPATRFSHELSLWELAGFFYGKHFYRPDS"
     gene            806..2743
                     /gene="asnB"
                     /locus_tag="VFV61_01990"
     CDS             806..2743
                     /gene="asnB"
                     /locus_tag="VFV61_01990"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEX4899271.1"
                     /translation="MCGIAGFVDKWDRNAGESFERGRLLKGMCDVIRHRGPDDDGFLL
                     KEGVALGMRRLSIIDLAGGAQPISGEDGSVTIVFNGEIYSFHELRSELEKRGHTFKTH
                     SDTEAIVHAYEEYGPSCADHLRGMFAFAIWDDKKRELYIARDRVGKKPLYYTVTPGQT
                     LVFGSEIKSILEHPDVKREINLEALDAYFTLGYVPDPLSIFQNIYKLPPGHHLTFSSG
                     RVAIQQYWDFNFERGESRRPEQDYLEELRALLDESVRLRLISDVPLGAFLSGGIDSST
                     IVALMARHMNQPVKTFSIGFHEDSYNELKYARLTAKKLGTDHHEFFVTPQICDIIDDL
                     VWHFDEPFADPSAIPTFMVSKLARDHVTVALSGDGGDELFAGYTHYVVHESRRAFSAL
                     PKALRAGVLRPLSYRLPHGAWGRNYLHNISLDPIDRYLDSLSYFTELGKESLYTSDFQ
                     KALQTKDQVARMFREYAGRVKTNEPLDQLLYIDGKTYLPGDILTKVDRMSMATSLEVR
                     VPLLDHKLIEFVSKVPADLKLAGTETKHLLKRVARDLIPSEILDRPKQGFGIPLEEWI
                     NDQLRDQIRDTLREPRTRQRGYVNSNYVDLILDEHHKGRRDHSFNLWGLLILELWHRR
                     YIDQSPRMSESDERDAVPVPV"
     gene            2807..>3623
                     /locus_tag="VFV61_01995"
     CDS             2807..>3623
                     /locus_tag="VFV61_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899272.1"
                     /translation="MQPRWVRKALENVVAAEVSTFELVVKVASEKDNGENLLHKLYNG
                     MDRSLWPTSPDALELVSIEDLVGSLPSLKSDELKQTDLDVLLSFGPTELNSRFANLAK
                     YGVWFYAFGIDEQAPPGFREVLTLDPIMICSVKSLNGQPPEERIIYKSVSPTLSRFSI
                     RRNNNECYWKSAAFFARGLQDLYHGRNLPEMGVADPTDSSLAPLTNAALGGMFLKLSH
                     RAASRALEKLSSFEQWVLAYRINQTEFKYLEPPADRFWADPFPIKVAGKYYIFF"
BASE COUNT          829 a          938 c          972 g          884 t
ORIGIN      
        1 aaggccaacg gccggatctc gtacgtgttt gagaaagcgc agggccgatc tccggcgctc
       61 aacgcgggtg tagctgctac tcatggcgac ttggtaggct tcatcgatga cgatgaagag
      121 gtggacgaat catggtacga agtggtgctc gctgccttcg caaacgaaga cgtagacttc
      181 attggcggtc cttacgtgcc gcggtttgaa agtgaagttc cgaagtggct cccaaaagat
      241 cataacgccg tagttgggat tgttgatggg ggtgacaaag tagtttcctt cgacgactcc
      301 tatccgggaa tgcttatggg aggcaacgca gttttccgac gtgaagtctt gcagcgagtc
      361 ggtccatact ccaccgccct cggccgcagc ggcacccgct tgttgtcgtg cgaagacgag
      421 gacatgtatc agcgtctgcg agaggccggt gcgcggggtc tctatctgcc gcaactgatc
      481 gtttatcatt tcattccaag cgagcgactg acaaaaagct atcatcgctc gtggtgtttc
      541 tggcagagtg tttccagcgc tgtgctggat cgaattcgcc cgaagccggt tgcgtatgtt
      601 ttaggggtgc cgcgttattt ttacggccga gcccttcgtg ggatgttacg cttggccaat
      661 ctacgcgaac gcgagcccgc cactcgtttc tcgcatgagc tatctttgtg ggagttggcg
      721 ggtttctttt atggtaaaca tttctaccgg ccggatagct gaggatttga gggaacggtt
      781 cctgtatttc tcctttagat aaattatgtg cggcattgca ggatttgtag ataagtggga
      841 ccgaaacgcc ggcgaatcat ttgaacgtgg tcgtctgctg aaaggcatgt gcgatgtcat
      901 cagacatcgc ggtcctgatg acgacgggtt cctactgaaa gagggagtcg ccttgggtat
      961 gcggcgtctc tcaatcatcg atcttgccgg tggcgcccaa ccgatttccg gtgaagatgg
     1021 ctcggtcact atcgtcttca atggcgagat ctacagtttt cacgaactca ggagtgagct
     1081 cgagaaacgc ggtcacacct tcaagaccca ttccgacacc gaagcgatcg ttcatgctta
     1141 cgaagagtat gggccgtcct gtgcggatca cctgcggggg atgtttgcgt ttgcgatctg
     1201 ggacgacaaa aaacgcgaac tttatattgc ccgggatcga gtgggaaaga agccgctcta
     1261 ctacacagta acgccgggac aaacgctggt gttcggttca gagatcaagt cgatcctcga
     1321 acacccggat gtaaagcgcg agatcaacct cgaagcactg gatgcgtact tcacactagg
     1381 atacgttcct gatccactca gcatctttca gaacatctac aagcttcctc caggacatca
     1441 cctgacgttc tcatcaggtc gagtggcgat tcaacagtat tgggatttca acttcgagcg
     1501 gggagagtcg cgtcgccccg aacaggatta tcttgaagaa ctccgcgcgc tgctcgatga
     1561 gtccgtgcgt ctgcgcttga tttcagacgt tcccctcgga gcctttctat ccggtggaat
     1621 agattccagc accatcgttg cgctgatggc gcgacacatg aatcaaccgg ttaagacgtt
     1681 ttccatagga tttcatgagg acagttataa cgaactcaag tacgcgcgcc tgaccgccaa
     1741 gaaactgggc acggaccatc acgagttttt cgttaccccg caaatctgcg acatcatcga
     1801 cgatctcgtt tggcactttg acgaaccgtt tgccgaccct tccgcgatac caaccttcat
     1861 ggtttcgaaa ctcgctcgcg accatgtgac tgtggcgttg tctggagacg gtggcgacga
     1921 attgttcgct ggttacacgc actatgtagt gcatgaaagc cgccgtgcct tctccgcgtt
     1981 gccgaaggcg ttgcgggcag gtgtgctgcg gccgctgagt tatcgtctcc cgcatggcgc
     2041 ctggggtcgc aattatcttc ataacatttc gctcgatccg attgatcgtt atctcgacag
     2101 cctttcctat ttcactgaat tgggtaaaga gtcgctttac acgagcgact tccaaaaagc
     2161 cctgcagaca aaggaccaag tggcgcgcat gttccgcgag tacgcgggtc gagttaaaac
     2221 aaacgaacca ctcgatcaat tgctttatat cgacggcaag acttacttgc ccggagatat
     2281 cctgaccaaa gtcgatcgaa tgagtatggc gacctcactc gaggtgcgcg tgccactgtt
     2341 ggatcacaag ctgatcgagt ttgttagtaa agttccggca gacttgaagt tggcaggcac
     2401 cgaaacaaag cacctgctga aacgagtggc cagggatttg atcccgtcag agattctgga
     2461 ccggcccaaa caaggcttcg gaataccgct cgaagaatgg atcaacgatc aattacgcga
     2521 tcagattcga gacacgttgc gcgaacctcg cacccgacaa cgcggctacg tgaactcaaa
     2581 ctacgtcgac ctcatcctgg acgaacacca caaaggacgt cgcgatcact cctttaatct
     2641 ctgggggctt ctgatactcg agttgtggca ccgccgctac atcgatcagt caccgcgcat
     2701 gtctgagtct gacgagcgcg atgctgtgcc tgtacccgta tagataatga cggcacacgt
     2761 cgctaaatct tcccttttaa gagtaggaat aattatcgac tcttttgtgc agccccgttg
     2821 ggtgcgcaag gccctcgaaa atgtggtcgc tgccgaggta tcgacttttg agctggtagt
     2881 gaaggtcgcc agtgaaaaag acaatggaga aaatctactg cacaagctct acaacggcat
     2941 ggaccgaagc ttgtggccca cttcgccaga tgccttagag ctggtaagta tcgaggatct
     3001 ggttggttcg ctgccaagtc tcaagtcaga tgagttaaag cagaccgact tggatgtact
     3061 tttgagtttt gggccaaccg agttgaattc gaggtttgcc aatctcgcta agtacggcgt
     3121 ttggttttat gcgttcggaa tagacgaaca agccccgcct ggcttcagag aggttttgac
     3181 tctggatccg atcatgatct gctcagttaa gtctctaaac ggtcagcctc cagaagaacg
     3241 gatcatttac aaatcggttt cacccacgct tagtaggttc tcaatccgcc ggaacaacaa
     3301 tgagtgttac tggaagtcag cggcgttttt cgcgcgcggc cttcaagacc tttaccatgg
     3361 acgcaacctt cctgaaatgg gggtcgctga tcccactgac agttccctag cgccgcttac
     3421 gaacgctgcg ctggggggaa tgtttctcaa gctctcccat cgagccgctt cacgagccct
     3481 tgaaaagctt tcatctttcg agcagtgggt gctcgcgtat cgcatcaatc agactgaatt
     3541 caaatacctt gagccaccag ccgatcggtt ttgggcagat ccattcccaa tcaaagtcgc
     3601 aggtaagtac tacatttttt ttg
//
LOCUS       DASUEX010000118         7483 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1513427, whole genome shotgun sequence.
ACCESSION   DASUEX010000118 DASUEX010000000
VERSION     DASUEX010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7483
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1513427"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(294..668)
                     /locus_tag="VFV61_02000"
     CDS             complement(294..668)
                     /locus_tag="VFV61_02000"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX4899273.1"
                     /translation="MKRVAVFISLGLGFSAVMFFATTSQRARVMSGSNAASGRQIHVK
                     NCQRCHGPQGKGDGPAGKLLKTKPADWTDKKKMSALSDAYLYKIIAGGGGAVGKSTLL
                     PAFKGKLNDNQIRDVISFIRSL"
     gene            complement(754..3231)
                     /locus_tag="VFV61_02005"
     CDS             complement(754..3231)
                     /locus_tag="VFV61_02005"
                     /inference="COORDINATES: protein motif:HMM:NF023282.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MtrB/PioB family decaheme-associated outer
                     membrane protein"
                     /protein_id="HEX4899274.1"
                     /translation="MRNQQNRRWSLGHLVVPLVLLLAPVSTLAQEQSTIAANSDHIVK
                     NEESKKADRAKEENKKKDQAKPSATPEPGPTGKGLGSNLNLLIDIGGQARSVTGERPS
                     KFEEYKRVREGFLFRRFRIASNPADSPKFFRLIGRGPSERDQQYLFDIGEYGRYRTTV
                     EWGQLPKLFSRGSRTPYVTQGNGLLIVSDQIKATLQPLLDVRSPTLPLVAGNIFRNSP
                     LINIGQTRETLSINQTFQVTSHWSVRARFLDYKRSGSKPLGIGAYERIATPNAEIGIT
                     TAIGDSFRILNLELPEPIDSRTDQITFGTSYVTRHWGVNFDYTFSQFNNRIESLTFDN
                     PFRLTDKQAVGGRNRLEFARGIYAEEPSNKSHSFLISAFVDLPGHSRLASALGWSFWK
                     QNAPFLPFTLNTAIVASNLPAGVSPTSLAALPRQSLEGDVDTFTQDHVFASRPAKNFS
                     FDIHYRSYDYNNTTPDMLFPGSAADSESSWLISLGTEPMENRPVSFHRQNFIAEGTWD
                     IAKWLKWKIEYQWEGWDREHRQVANSNEHTFGTQLSYKPNKRFDSKLIYRYSYRTPRA
                     YDPGVLEFNQLRVFDQAKRIRHNANLQWQYSVTPKLGLSGTFSYLRDNYDKNFFGLVR
                     YLQGQGSVDLLYTPNETTTFYANYSRERYSSLLQSIAKTGAPFDFRNRWNREDRNSND
                     SFGIGVTSYFAKGKLLLDANYALSLSNDRITTANLTAVSPTAVLNATAFPFPDVKSNY
                     HELNVDTSYQLTDNVALGFRYIFEPYRLDDFQWNGLNAYPLDRLPPQEQYPTSRPLLL
                     DSRYSSHNAHVFGIYLRFSKGKNAGTP"
     gene            complement(3262..4260)
                     /locus_tag="VFV61_02010"
     CDS             complement(3262..4260)
                     /locus_tag="VFV61_02010"
                     /inference="COORDINATES: protein motif:HMM:TIGR03508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DmsE family decaheme c-type cytochrome"
                     /protein_id="HEX4899275.1"
                     /translation="MSKRPQKFRTLMRAILLLTLFLFIPAFFVSSGRNLSGPADNPRA
                     PQAADENYVGSETCKLCHEDQFKNFSHTVHAKIADAKDWKGKVTGCETCHGPGKAHVD
                     GGGDTAKIKNPAKMKPEEVSATCQTCHAQMNEHAIWRGSKHESAGLSCVSCHSSHATA
                     GANPGQLFANMQSETKLLKMRTQAETCFQCHGDMRKAQFQRSTHFFRNEDRESRMTCS
                     TCHEPHGSIGVKMMRTGNINETCYTCHAEKRGPFLWEHSPVRENCMTCHKPHGSNNTN
                     LLIARAPMLCQQCHIQGRHQTVAGRPNSGFVMNRACLNCHSQVHGSNHPSGILLQR"
     gene            complement(4570..5937)
                     /locus_tag="VFV61_02015"
     CDS             complement(4570..5937)
                     /locus_tag="VFV61_02015"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX4899276.1"
                     /translation="MDEAHILVADDDRGILHTCRKILEHAGYEVTTAADGKAAISLLK
                     ASHYDLFLVDLKMPALNGLEAVSLARALDPALMIVMFTAHGTIETAVEAVKRGAFDYL
                     TKPFTSDQLRLAVERALRQKQLLEENLNLREQLATELGFDKIVGTSEAMRKLFATLQK
                     VVRSNANILIQGETGTGKDLVARTLHAHSFRRDRPFVAVDCAALPENLLESELFGHEK
                     GAFTGADRVIRGQLELAHTGTLFLDEIGELSFALQAKLLRTLQEREFRRLGSEHQILV
                     DIRVISATSRDLRTEIAARSFRQELYYRLNAVTVNLPPLRDRKNDIPILAHHFLEHFC
                     RECGLTWLDVSPDVRKLFLDYGWPGNVRELQNVVQHAVLMAETKTIGICDLPDYLRTE
                     PSEQLSFHEVRSKEAESVEKPFLEELLRKHRGNISKAADEAGLSRKTVYRLAKRFNVD
                     LHEFR"
     gene            complement(5940..>7483)
                     /locus_tag="VFV61_02020"
     CDS             complement(5940..>7483)
                     /locus_tag="VFV61_02020"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX4899277.1"
                     /translation="GLDLANLILITLALTRIYRHPLGGRKFVAGALALISLAAALHFT
                     SSLKWGSDTSLLFWNAEQFAWSFSLITCALAMGETSRNLFDKVFVRLQITFILLASVM
                     LLVITQTEKSDYFAGIRGRADHLVEFVREHIDYLRQQNQPLSAIIAQEDISKAIFGFG
                     NLPELKVVRIMAGSEVATFEVDNEGKVVSRLKSIESASPATRFDTEEYFLIRALPLKV
                     SPPGEVAFYGTREFLDEHIRRRIVIIFSLFTAMVALSTLMIGLVVRDASTTIREQARE
                     IGKTQEQLSQASKLAAIGELAAGVAHEINNPATTILSLSSFWVSQNGAKSVAVDQEDL
                     QEVMTQAQRIAKITTSLLEFSRPQVSALSRVPLDRVINLGLRLVDDLLSANHISVEKR
                     LRARIPLVSGDEDSLVRALENLFRNAIDAMPHGGALRVNLVRDDKSSNRMRLEISDTG
                     VGIEKDNLDRIFDPFFTTKEVGKGTGLGLSIVHGIIKEHHGTITVESNPGQGTKFTIA
                     LPTEV"
BASE COUNT         1697 a         1996 c         2001 g         1789 t
ORIGIN      
        1 aaagctatcg gttgggtcac catgcggttg tacgagcgaa cgacgaacaa tccgctcaag
       61 gtaaatattg caaccggcag gacggcgaaa ataattatcc gcttcatatc tttttcctga
      121 cgtcagtcag aaggggccag ttcgttgtct catgtagctc tgcgggtctc cgtggtctct
      181 ggattgaaat cctacccggc agaacctcaa ccgcagagac acggagatcg cacagggcct
      241 taggaagaaa ccaaagccgc caagacgcgt caaagccaag cgatgcgtat caactacaga
      301 ctgcgaatga agctgatgac gtcgcgaatt tgattgtcgt tcagcttgcc tttgaacgcc
      361 ggcaacagcg tagatttacc gacggctccg ccaccgcccg caatgatctt gtacagatag
      421 gcgtccgata gcgccgacat ctttttcttg tcggtccagt cggctggttt tgttttcaga
      481 agcttgccgg cgggtccgtc tcccttgccc tgtggtccgt gacatctctg gcaattcttt
      541 acgtgaatct ggcgaccgct ggcggcattg cttccagaca tgacccgggc cctttgagac
      601 gttgtcgcaa agaacatgac tgccgagaat ccaaggccga gagaaataaa cactgcaact
      661 cttttcacaa ttgctcacct ccatcgagat tgttgaatcc agacgatcag gccaacgaac
      721 agccgattgg cttcacctaa gtgtgttaaa taactagggt gtgccagcat tctttccttt
      781 cgagaagcgc aggtaaattc cgaacacatg tgcattatgg ctcgagtagc gcgagtccag
      841 cagcaacgga cggctggtcg ggtactgctc ctggggagga agccggtcaa gcggatatgc
      901 atttaacccg ttccactgga aatcatccag gcgatacggc tcaaaaatgt agcggaaccc
      961 cagggcgacg ttgtcggtca actggtagct cgtatccacg tttagttcat ggtagttgct
     1021 cttcacgtcc ggaaacggaa acgcggtagc gttgaggact gcggtcgggg acaccgcagt
     1081 caaattcgcc gtcgtaatcc gatcattaga gagcgagagc gcgtagttgg catcgagaag
     1141 caatttgccc ttagcaaagt aggaggtgac gccaatacca aagctgtcat tcgagttgcg
     1201 atcctcccga ttccatctgt ttctaaaatc aaaaggtgct cccgtcttgg caatgctctg
     1261 cagcaaagag ctataacgct cgcgcgaata gttggcgtaa aaggtggtgg tctcgtttgg
     1321 tgtgtagagt aaatcaacac tcccctgccc ctgcaggtat ctcaccaacc caaagaagtt
     1381 cttgtcgtaa ttgtcgcgca gatacgagaa ggtgcccgag agtcccagct ttggcgttac
     1441 tgaatattgc cattgcagat tcgcgttatg acgaatgcgc ttggcctggt cgaagaccct
     1501 gagctggtta aactccagta cgcccggatc ataagcgcga ggtgttctat aggaatagcg
     1561 gtagatcagc ttgctatcga acctcttatt gggcttgtaa ctaagctgag tgccaaacgt
     1621 atgctcgttc gaattagcta cttgccggtg ctctctgtcc catccttccc actggtactc
     1681 aatcttccac ttcagccact tcgcgatgtc ccaggtgcct tcggcaatga agttctgtct
     1741 atgaaacgac accggcctat tttccatagg ttcggttccg aggcttatga gccaggacga
     1801 ttcactatct gccgcggagc ccgggaagag catgtccggc gtggtgttgt tgtagtcgta
     1861 ggaacgataa tgaatgtcaa acgagaagtt ttttgcgggt cgggacgcaa agacatgatc
     1921 ttgtgtgaaa gtatcaacgt caccctcgag actctgacgc ggcaatgccg caagcgatgt
     1981 cggcgacaca cccgccggca ggttactagc cactatcgcc gtattgagag tgaaaggaag
     2041 aaacggcgca ttctgtttcc agaaactcca accgagcgca ctggcgagcc gcgaatggcc
     2101 cggcaaatcc acgaaggccg agatgaggaa actgtgggac ttgttgctgg gctcttcggc
     2161 atagatgccg cgcgcaaact ccaggcggtt acggcctccc acggcctgtt tgtcagtgag
     2221 tcgaaatgga ttgtcgaagg taagagattc aatccgattg ttgaactgag aaaaagtgta
     2281 atcgaagttg accccccagt gacgcgtaac gtagctcgtt ccaaacgtta tctgatctgt
     2341 gcgactgtcg atcggctccg gcaattccag attgagaatg cggaaagagt ctccgatcgc
     2401 ggtagtgatg ccgatttctg cattcggagt ggcaatgcgc tcataggcgc cgatgccaag
     2461 aggtttcgat ccggagcgtt tgtaatcaag aaatctggcg cgcacgctcc aatgcgaagt
     2521 cacttgaaat gtctggttaa tgctcaacgt ctcgcgggtc tgtccgatat tgataagcgg
     2581 cgagttgcga aagatattgc cggctaccaa tggcagagta ggtgatctga catccagcaa
     2641 aggttgtaat gtggccttga tttgatccga cactataagg agcccattgc cttgcgtcac
     2701 atagggcgtg cggctcccgc gtgaaaaaag cttcggcaac tggccccact cgacagtggt
     2761 tcgatacctg ccgtattcac cgatgtcaaa caggtactgc tgatctctct cagacggccc
     2821 tcttccgatc agccgaaaga atttcggcga gtctgccggg ttcgaagcga ttctgaacct
     2881 tcgaaacaaa aagccttcgc gtacgcgctt gtactcctca aacttcgacg gtctttcgcc
     2941 ggtaacactg cgcgcttgcc ccccgatatc tatgagcaga ttcagattac tgccaaggcc
     3001 ctttccagtg ggtccgggct caggcgtcgc cgagggtttc gcctggtctt tcttcttatt
     3061 ctcttccttc gctcgatcgg ccttcttgct ttcctcattc ttgactatgt gatcactatt
     3121 ggcagcaatg gtgctctgct cctgggccaa ggtggaaaca ggcgccagca agagaacaag
     3181 gggtacaacc aaatggccca gcgaccatct tctgttttgt tggtttctca ttgtcttctc
     3241 ctctctattc tcgctgtgcg cttaccgttg caggaggata cctgaaggat gattcgagcc
     3301 gtggacctgc gagtggcagt tcagacatgc tctgttcatg acgaagcctg aatttggtct
     3361 gccggcgacg gtctggtgcc ggccttggat gtgacactgt tggcagagca tcggcgctct
     3421 ggcaatcaac aagttggtgt tgtttgagcc gtgcggctta tgacaggtca tgcagttctc
     3481 gcgcactggt gagtgttccc agagaaaagg cccacgtttt tcagcgtggc aggtgtagca
     3541 agtttcgttg atgttgccag tgcgcatcat ctttacgccg attgatccgt gcggctcgtg
     3601 acaggtagag caagtcatgc ggctctctct atcttcgttg cggaagaagt gcgtcgaacg
     3661 ctgaaactgc gctttgcgca tgtcgccgtg acactggaag cacgtctctg cctgggtcct
     3721 catcttcagc agtttggttt ccgactgcat attggcaaag agttggccag ggttagcccc
     3781 tgctgtcgca tgcgaactgt ggcagctcac acaggaaagc ccggcactct cgtgcttgct
     3841 acctcgccag attgcatgct cgttcatctg agcgtgacac gtctggcaag tcgcgctaac
     3901 ctcctccggt ttcatcttcg cagggttctt gatcttcgcc gtgtcgccgc cgccatctac
     3961 gtgggccttg cccgggccat gacacgtctc acagccagta actttgccct tccagtcctt
     4021 tgcgtcagcg atcttggcat ggactgtgtg agaaaagttc ttgaactggt cttcgtgaca
     4081 gagcttgcaa gtctcgctgc cgacgtagtt ttcgtccgcg gcttggggcg ccctcgggtt
     4141 gtctgccggt ccgctgagat tgcgtccaga agagacaaaa aaggccggga tgaataagaa
     4201 cagagtaagc agaagaattg ctcgcataag ggttctgaac ttctgaggtc gcttggacat
     4261 aggaagtcct ccttgtgaat tcaccaactt tgcgggcccg atcttgttcg caccgacgat
     4321 agccaaagct ctcgccgctt cagcgaccgc atagtcaaat cgcgatccac gctgcgcgtg
     4381 atattagtga gaaaccgtaa atgatgcaga atcagtagct tggaaggaca ttgatttctg
     4441 gccagcaggg gtgtcgcggg ccgatttgtt tactttggta tccagataca agagcacacg
     4501 acggaatttc caagtattca agaagaatcg aggtgcggat ggtaaacaac agtgtgacac
     4561 ttggggtatc taacggaact cgtgtaaatc gacgttgaat cttttagcca ggcgatagac
     4621 cgtcttgcgg gacagtccgg cctcgtcagc ggccttcgag atattgcctc tgtgttttct
     4681 gagcaactcc tccaggaagg gcttttcaac cgactcggct tccttactgc ggacttcatg
     4741 aaatgagagt tgttcggaag gctcggttcg caaataatca ggtagatcgc aaatccctat
     4801 tgttttggtt tccgccatga gaacagcgtg ttggacgaca ttttgaagtt cacggacgtt
     4861 acccggccag ccgtaatcaa gaaatagctt tcttacatcg ggactaacat cgagccatgt
     4921 cagaccacat tcgcggcaga aatgctcgag gaagtggtgg gccaggatcg gaatgtcatt
     4981 ctttcgatca cgcagcggag gaagattgac cgttactgca ttcaaacggt agtagagttc
     5041 ctggcggaaa ctccttgcag cgatttcagt acgcagatcg cggctggtgg ctgagatcac
     5101 tcgaatgtcg accaggatct ggtgctcgct tcccagccgg cggaactcac gctcttgcaa
     5161 tgtccgcaat agcttggcct gaagcgcgaa cgagagttca ccaatttcgt ccaggaacag
     5221 cgttcccgta tgagccaact ccaactggcc tctgataacc cggtcggcgc ccgtaaatgc
     5281 acccttctca tggccgaaaa gctcgctctc caacaggttt tcgggcaagg cggcacaatc
     5341 aaccgcgaca aagggtcggt ctcggcggaa actgtgcgcg tggagagtgc gcgcaaccag
     5401 gtccttgccg gtcccggtct ctccctggat cagaatgttg gcattgctgc gcaccacctt
     5461 ctgtagcgtc gcgaagagtt tccgcattgc ttcgctggta cctacaatct tgtcgaaacc
     5521 cagctcggta gctaactgct cacgcagatt aagattctct tccagtagtt gtttctgccg
     5581 taaagctcgc tcaaccgcta accggagttg atcagacgtg aaaggcttgg ttaagtaatc
     5641 gaaggccccg cgcttgaccg cttcaactgc cgtttcgatc gtcccatgcg cggtgaacat
     5701 gacgatcata agtgcggggt cgagtgcgcg agctaagctc acagcttcca gcccgttcag
     5761 ggccggcatc ttgagatcca ctagaaacaa atcataatgg ctggctttca gcagactaat
     5821 tgcggccttg ccgtccgctg cggtggtcac ctcataaccc gcatgctcca gaatctttcg
     5881 gcacgtgtgc agaatgccgc gatcatcgtc cgcgaccaga atgtgcgcct cgtccatagt
     5941 cacacctccg taggtagagc aattgtgaat ttggtgccct gacccggatt gctctcaacg
     6001 gtaattgttc cgtgatgctc tttgatgatg ccgtgcacga tcgagagtcc caggccagta
     6061 cccttgccta cttccttcgt cgtgaaaaat ggatcaaaga ttcgatcgag attatccttt
     6121 tcaattccca caccggtatc ggaaatctcc agccgcattc ggttggacga tttgtcgtcc
     6181 cggacgagat ttacccgcag ggccccgccg tgcggcatgg cgtcgatggc gttgcgaaag
     6241 agattttcca gtgcccgcac gagactgtct tcatcgccgg acacaagcgg gatacgagct
     6301 cgtaaacgct tttcgacgga gatgtgattg gcactcagca aatcgtcaac taaccgcaag
     6361 cccaggttga taacgcgatc gagcggcact cggctaagtg ctgagacctg cggacgcgag
     6421 aactcaagca gcgaggttgt tattttcgca atgcgttggg cttgcgtcat tacctcctgc
     6481 aagtcttcct ggtcaacagc aactgacttc gcgccgtttt gcgaaaccca aaaactcgac
     6541 agacttaaaa tagtagtcgc cggattgttg atctcgtgag ctacgcctgc tgccaattca
     6601 ccaatcgctg ccagtttgga cgcttggctc agttgctcct gtgtcttccc gatctcgcgc
     6661 gcctgctccc taatcgttgt gcttgcatcg cgcactacca aaccaatcat gagcgtcgaa
     6721 agcgcaacca tggcagtaaa gagagaaaag atgatgacaa tccggcgccg gatgtgttca
     6781 tcgagaaact cacgcgtgcc gtagaacgcc acttcgccgg gcggcgagac ctttagagga
     6841 agcgcgcgaa ttaggaaata ttcttccgtg tcgaaccgcg ttgccggcga ggctgattca
     6901 atgctcttca gcctcgagac cactttgcct tcgttgtcga cttcgaacgt ggccacctca
     6961 ctgccagcca tgattcgaac tactttcaat tcaggcaagt taccgaatcc aaagatcgcc
     7021 ttcgaaatgt cttcctgcgc gatgatagcc gacaagggct gattttgttg ccgaagatag
     7081 tcgatgtgtt cgcgcacaaa ctccacaaga tgatcggcgc gaccacgaat gccggcgaag
     7141 taatcggatt tttcggtctg ggtgatcact agaagcatca cgctggccag cagaataaaa
     7201 gtgatctgca atcgaacgaa tactttgtca aacaaattcc tgctggtctc gcccatcgcc
     7261 agcgcgcaag tgatgaggga gaatgaccag gcaaactgct ccgcgttcca aaacagaagt
     7321 gaggtgtcac ttccccactt taacgacgag gtaaagtgca gtgcggcggc tagagagatc
     7381 agcgccagcg cgcccgcaac aaacttgcgg ccacccagcg gatgtcggta aatgcgcgtc
     7441 agcgccagcg ttattagaat tagatttgcg agatccaggc cgg
//
LOCUS       DASUEX010000119         2471 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1392484, whole genome shotgun sequence.
ACCESSION   DASUEX010000119 DASUEX010000000
VERSION     DASUEX010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1392484"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(168..581)
                     /locus_tag="VFV61_02025"
     CDS             complement(168..581)
                     /locus_tag="VFV61_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899278.1"
                     /translation="MGAWLSDSTIRDYFFALGLGAALAGAELAAGDAAGDGVSVAVGE
                     GCCTGADSLSGAADCSTERWPVIAGNESMSASNINAAAAPMVILLRMLCVPRGPKAVL
                     ETLLEKSAPASALPGCSKTVTTKTTQERINSPYKK"
     gene            615..699
                     /locus_tag="VFV61_02030"
     tRNA            615..699
                     /locus_tag="VFV61_02030"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:649..651,aa:Leu,seq:gag)
     gene            complement(775..>2471)
                     /locus_tag="VFV61_02035"
     CDS             complement(775..>2471)
                     /locus_tag="VFV61_02035"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HEX4899279.1"
                     /translation="KVMMLERGTWWVTPAKIGKAPPSPRPSIPAWAKTQQPPQPVQYW
                     TRPDHKDGMLDFFAAIRNRFNKDGLYQYSMFKQADILTASGVGGGSLIYSNVTLRPQL
                     EVLQGIGLSLGDPEYQAAYKWMEDFRGKLNHIVTKIPLPGRDVTNLTTDDYLYLDRAR
                     VLRDAAKLSGTKLGIELPWAPLDLSLIEYDPHRPDGGDAAKVGTFCQREGRCILGCLP
                     SARHTFSKTLYTKLLSDPTKGVTLSPLSEVRDIKQIAGGYEVTYRDHRDDNKKTVSAP
                     LVFLAGGTLGTTEVLLRSRDKGGLKLSAKLGTHFSTNGDFGAFCVGTTKPVHSTRGPI
                     NTSGIHAKFDGLHIHVEDAGIPAMFAAIASTTIGVLDNFAQREALKAKMKFAFTSMAM
                     PDLRDFFPGLPDSYDPDSFQTEAEMVANIFFFNVMGQDDASGKFTLDNDNLKLDWDKK
                     IGETPIFGKIETLLKSLSESMGGRYVPFPFWKGLGDKKLTVTHPLGGCPIAPSSFDGV
                     VDELGRVFDGNKPAGSKDVYPGLFVVDGSAIPGALAINPTLTIAAQALKSLNAALP"
BASE COUNT          518 a          663 c          698 g          592 t
ORIGIN      
        1 gcgagtttga aagccgacag ctttgcgcag attgttaact accggaacgg atgagaaccg
       61 gggaacaggg ggaacagtaa gacgggtagt atcgatttac gattcacgat ttacaattca
      121 cgattcacga ttcacgattc acgatttaga ggtgcttact tgctaactta ttttttatac
      181 ggactgttta ttctttcctg tgttgtctta gtagtgactg tcttgctgca acctggcaaa
      241 gctgatgccg gcgcgctttt ctcaagcagt gtttccagta ccgcttttgg cccgcgagga
      301 acacaaagca ttctgagcaa gatcaccatt ggcgccgcgg cggcgtttat gttgctggcg
      361 ctcatgcttt cattgccggc tattaccggc caacgctcag tactgcaatc agcggcgccg
      421 gagagcgagt cagctcctgt gcaacagcct tcgcccacag cgaccgagac tccgtctcca
      481 gcagcgtcgc ccgcagcgag ttctgcgcca gcgagcgctg caccaagtcc cagcgcgaag
      541 aagtagtcgc gaattgttga atcgcttagc caggcgccca cggcgggacg ccgctacgat
      601 tcgatttgat ctttgccgaa gtggcggaac tggtagacgc gcacgtttga ggggcgtgtg
      661 aggtaactcg tgggggttcg agtcccccct tcggcaccag cccttagttc accgaatgga
      721 aaaccccgaa ttaacgctgt gctttgttca acgacaacgt tccaaaaacg aatgttaagg
      781 cagcgcggcg ttcagcgact tcaatgcctg cgccgcgatc gttagcgtcg gattgatcgc
      841 cagtgctccg ggtattgctg acccatccac gacgaagaga ccgggataca cgtccttaga
      901 tccggctggc ttgtttccat cgaacacccg accgagttcg tctaccacac catcgaaact
      961 ggacggtgct attgggcaac cacccagcgg atgagtcact gttagtttct tgtccccaag
     1021 gcccttccag aatgggaaag gtacgtagcg accacccatc gactcggaaa gtgacttgag
     1081 cagcgtctca atttttccga agattggtgt ctcgccgatc ttcttgtccc agtcgagttt
     1141 tagattgtcg ttgtccagag tgaatttgcc gctggcatca tcctggccca taacgttgaa
     1201 gaagaagatg ttggcgacca tctcagcctc ggtctgaaag ctgtcgggat catagctatc
     1261 gggcaggcca gggaaaaagt cgcgcaggtc aggcattgcc attgacgtga aggcgaattt
     1321 cattttggct ttgagtgcct cgcgttgcgc gaagttgtcg agcacgccga tggtggtgct
     1381 ggcaatcgcc gcgaacatcg ctggaattcc cgcatcctcg acgtgtatat ggaggccatc
     1441 gaacttggcg tggatgcccg acgtgtttat tgggccgcgt gtgctgtgga ccggcttcgt
     1501 cgtccctaca cagaacgctc cgaagtcgcc attagttgaa aagtgggttc ccagcttggc
     1561 gctaagttta agtccaccct tgtcgcgcga acgcagtaag acttcagttg ttcccagggt
     1621 gcctcctgcg agaaaaacca gtggcgcgga gacagttttc ttgttgtcgt cgcgatgatc
     1681 ccgataggtt acttcatagc cgcccgctat ctgtttgatg tctctcacct cggacagggg
     1741 cgagagagtc actcccttgg tggggtctga aagtaacttt gtgtagagcg tcttgctgaa
     1801 ggtgtggcgc gcagacggca aacaacccag gatgcagcga ccctcgcgct ggcagaaagt
     1861 tccgaccttc gccgcgtcgc cgccatccgg acggtgtgga tcatactcga tgagcgaaag
     1921 atcgagcgga gcccagggaa gctcgatacc gagtttagtt cctgagagtt tggctgcatc
     1981 gcgcagaacg cgggccctgt cgagatagag ataatcgtcg gtggtcaaat tcgtcacgtc
     2041 ccgaccaggc agtggaatct tggtgacgat gtgattgagt ttgccgcgaa agtcttccat
     2101 ccatttgtag gccgcttgat attctggatc accgagactg agtccgatgc cttgcaacac
     2161 ctccagctgg ggacgaagcg ttacgttcga ataaatcaat gatcccccgc caacaccgct
     2221 ggctgtcaga atatccgcct gcttgaacat cgaatattgg tagaggccat ccttattgaa
     2281 tctgttgcgg attgcggcga aaaaatcgag catgccgtcc ttgtgatctg gccgtgtcca
     2341 gtattgcact ggctgcggtg gctgttgtgt tttggcccat gccgggatgg acgggcgcgg
     2401 gctcggcggc gccttaccaa tcttggctgg cgttacccac caggttccgc gttcaagcat
     2461 catcactttc t
//
LOCUS       DASUEX010000120         2650 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_483212, whole genome shotgun sequence.
ACCESSION   DASUEX010000120 DASUEX010000000
VERSION     DASUEX010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483212"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            262..1098
                     /locus_tag="VFV61_02040"
     CDS             262..1098
                     /locus_tag="VFV61_02040"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX4899280.1"
                     /translation="MVTAPSTEIFSDISLARRLERTEARSNASFVEARARAFPEIGAE
                     WIDVCGAYAMFDGNESPLTQTFGLGLFDPMSENDLERIEKFFQERGAPVFHEVSPLAD
                     PAIFPLLNKRGYQPCEFTTVLYRSIERGTTFTELRNDDKIQVRLTTPAEVELWAQTSS
                     RGWSEFPELAEFMLRFGKVVAQRTDGLAFIAELAGKPIATGAMSIYDDVALLAGASTI
                     PEGRKQGAQLALLDSRLRYAAEHGCTIATMGALPGSASQRNAERQGFRIAYTRTKWEL
                     AG"
     gene            1248..>2650
                     /locus_tag="VFV61_02045"
     CDS             1248..>2650
                     /locus_tag="VFV61_02045"
                     /inference="COORDINATES: protein motif:HMM:NF014044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEX4899281.1"
                     /translation="MQDFEKLGVFYLGRPYDLKSKKPKNGLLLYDSKDLVTHAVCVGM
                     TGSGKTGLCMALLEEAAIDGIPAIVIDPKGDLANLLLTFPALKPEDFAPWINEDDAQK
                     KGLSPADYATQQAGFWEKGLAEWGQDRDRIKRLREAAEFRVYTPGSNSGIPVSVLKSF
                     AAPPATIREDNELMRERVNTTATSLLGLLGIDADPIKSREHILLSNILNNAWAGGNDL
                     DIAALIQQIQTPPLTRIGVMDLDSFFPAKDRFELAMSLNNLLAAPGFSSWMEGDPLDV
                     QQILYGPAGKPRVSIFSIAHLSDAERMFFVSLLLNQTLGWMRTQSGTTSLRAILYMDE
                     IFGYFPPVANPPSKLPLLTLLKQGRAFGLGVVLATQNPVDLDYKGLSNTGTWFIGRLQ
                     TENDKNRVLEGLEGVAAGSDLKFDRQQMEETLAGLSSRIFLLNNVHDDGPEVFESRWA
                     MSYLRGPLTRSQIKTLM"
BASE COUNT          594 a          706 c          738 g          612 t
ORIGIN      
        1 gcgaatgtgg catccgtatc tggtttacaa gctgcgccgc ttagtgcgca aacgggagat
       61 atgagatcgc cacagatgag cgcagcttcg acgcgaataa attaatccta aaagctcttg
      121 ttctttgatc cgcgagaatc gttgtatatc tgtggctgaa ttcatttggg atgtccagcg
      181 ttagtcggtt gcgcacagcg tacaaaaaag acaaccaaag cccggcggca ggtcggcctt
      241 ggtgaccatg aggtttcttc gatggtaact gctcccagca ctgaaatctt ttctgacatt
      301 tctttggcgc ggcgtttgga gcgaactgaa gcacgttcca atgcaagttt tgtcgaagcc
      361 cgcgcccgcg cttttccgga aatcggagct gaatggatcg acgtctgcgg tgcctatgcg
      421 atgtttgatg gcaacgagtc gcctttgacg cagacctttg gactgggctt gttcgatccg
      481 atgtcggaga acgatctgga gcgcatcgag aagttttttc aggagcgcgg tgcccccgtg
      541 tttcatgagg tgagcccctt agcggacccg gccatctttc ctctcctgaa caagcgaggc
      601 tatcagcctt gtgagttcac gaccgttctc taccgttcca ttgaaagagg taccacgttc
      661 acggaactgc gcaacgacga caagattcaa gtgcgtctga cgactccggc tgaagtcgaa
      721 ctctgggccc aaacatcttc gcgcggctgg agtgagtttc cagagttggc cgagttcatg
      781 ctgcggtttg gcaaagttgt cgcccagcga accgatggac tggcgttcat tgcggagttg
      841 gctgggaaac cgattgctac cggcgcgatg agcatctacg acgacgttgc tctgctggcg
      901 ggagccagca cgatcccgga agggcgaaaa cagggagcgc aattggcgtt gctcgacagt
      961 cgtctgcgct atgcggccga acatggttgc accattgcaa ctatgggcgc gcttccgggc
     1021 agcgcctcgc aacggaacgc cgagcgccag gggttccgca ttgcttacac tcgaacaaaa
     1081 tgggagttgg ctgggtagag gacaaggact tcgatttccc attgtagatt ggcgattgcc
     1141 gattcctttc gttaactaca ggaatgaatc tcaattggca tttggcaatc ggcaatctat
     1201 tatctatccg caccaacccc ccatttcaca ctgaaaggtt tccgccgatg caggacttcg
     1261 aaaagctggg tgtcttctat cttgggcgtc cttacgatct aaaaagcaaa aaaccaaaaa
     1321 acggtctcct gctttacgac tcaaaggacc tcgtcacgca cgcggtctgc gtcggcatga
     1381 cgggcagcgg gaagaccgga ctgtgcatgg cgctgcttga agaggcagct atcgatggca
     1441 ttcccgccat tgtgatcgat cccaaaggcg atctcgcaaa cctgctgctt acctttcccg
     1501 ctcttaaacc ggaagacttt gccccctgga tcaacgaaga cgacgcccaa aagaagggac
     1561 tctcgccggc agactatgcc acgcagcagg caggtttctg ggaaaaaggt ttggcagaat
     1621 ggggtcagga tcgggatcgt atcaagcgct tgcgagaggc cgccgagttt cgcgtttaca
     1681 cgcccggtag caattccggg atcccggttt ccgtgctcaa gtcgttcgcc gctccaccag
     1741 caacgattcg ggaagacaac gagttgatgc gcgaacgagt aaacactacc gccacgagcc
     1801 tgctcggtct attgggcatc gatgctgacc cgattaagag ccgcgaacac attttgcttt
     1861 ccaacatcct caacaacgcc tgggcaggcg gcaatgatct ggatatcgcg gcgctaattc
     1921 agcagattca gacgccacct ctgacacgca tcggtgtgat ggatctggac tccttttttc
     1981 cggccaagga tcgttttgag ctggcaatgt cgctcaacaa cctgctcgca gctccgggct
     2041 tcagtagttg gatggaaggc gatcccctcg acgtgcagca gattctttat ggcccggcgg
     2101 gaaagccgcg agtttcaatt ttctctatcg cgcaccttag cgacgccgaa cgcatgtttt
     2161 ttgtttccct cctgctgaac caaactctcg gttggatgcg aacccagtct ggcactacga
     2221 gcttgcgcgc gatcctatac atggacgaaa tcttcggcta cttcccgccg gttgcgaacc
     2281 cgccgtcgaa gctgccgttg cttaccttgc tcaaacaggg gcgggctttt ggattgggag
     2341 tggtattggc cacacagaat cctgtcgacc tggattacaa aggcttgtcc aacaccggca
     2401 cctggttcat cggacgctta caaactgaga atgataagaa tcgtgtactc gaaggcctgg
     2461 aaggagtggc ggcgggtagt gatttgaaat tcgatcggca acagatggaa gaaacgctgg
     2521 ctggcttaag cagccggatt ttcctgctga acaatgttca cgatgatggt ccggaggttt
     2581 tcgaaagtcg ctgggcaatg tcgtatctcc gtgggccact tacccgaagt caaatcaaga
     2641 cattgatgga
//
LOCUS       DASUEX010000121         2655 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_712278, whole genome shotgun sequence.
ACCESSION   DASUEX010000121 DASUEX010000000
VERSION     DASUEX010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_712278"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(117..1181)
                     /locus_tag="VFV61_02050"
     CDS             complement(117..1181)
                     /locus_tag="VFV61_02050"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX4899282.1"
                     /translation="MLLIPGVALLGLTLTQIPLSSAQSQNQQRPRLVESTSRTKPEAK
                     PTPPVDAADSDAEVIRVDTDLVNTVFTAVDQDRHFVTTLRANDLRVLENQTPQDISLF
                     ERETNRPLTIVILVDTSKSQERTLPDEKKAAKAFLGAVMRPAVDKVAIVSFTGAPTVQ
                     QDLTDSFPKLERAIDRLRVELPPDNPECENVKTPQEDPLCWTSIWDSVWASANEVLAK
                     APENSRRAIVLLSDGDDTASTIKRQESIDSSVRNNVAVYSIGIGDPQFYKIEKDSLEK
                     LSNRTGGRAFFPSSEPELQRAFTQIQEELRSQYVVAYSPKNKARDGARREVKLEIVNP
                     ELRKQKLQLLYRQNYYAPKR"
     gene            complement(1293..1862)
                     /locus_tag="VFV61_02055"
     CDS             complement(1293..1862)
                     /locus_tag="VFV61_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899283.1"
                     /translation="MIMTNKLLPVALIAALVGGSVGALVMHTRNQSNTAAATERATVP
                     TDATYVADRQVTPDPFMPAAFDTAEEQGAYKVGFADGFRAAQAGVEGARSVTPAQVSY
                     APRRTVYRNSGRSYANSTNSRSVYSQPRSRSFWDKHRDKLTVAIGAGSGAAIGGLIGG
                     KKGAGIGALAGGGGSALYTYKLRNRSRRY"
     gene            complement(2164..>2655)
                     /locus_tag="VFV61_02060"
     CDS             complement(2164..>2655)
                     /locus_tag="VFV61_02060"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX4899284.1"
                     /translation="FTADPKATVFLGPVVVLIDTGTAGAAEVVASALLERNRGQIVGE
                     KSFGAGAEQQLFTLRGGDGLLLTTVKWASASGKPFLGEDRAHSGVTPSVEVKGPELAE
                     SVDPEDLTGNDDDAVAKPAQPGEKRDVAPEPNTPKPADDVQMKKALELLRENKPAQTQ
                     RAA"
BASE COUNT          591 a          747 c          709 g          608 t
ORIGIN      
        1 tgatgaggtg attgagccgg cgcaaacaag accaaaactg attcgggcgt tacagctgct
       61 ggaaaacaaa cgcgacacaa acccgccgcg gaagcacggc aacatcccac tctgaactaa
      121 cgcttgggcg catagtagtt ttgccggtaa agcaactgca acttttgctt acgcagttcc
      181 gggttaacga tctcaagctt cacttcccgc cgtgctccat cacgagcctt attcttgggc
      241 gagtaggcaa cgacgtattg cgaccgcaat tcttcctgga tttgcgtgaa cgcgcgctgc
      301 agctccggct cactggaagg aaaaaaagcg cgacctccgg ttcgattaga gagcttctct
      361 aaggaatcct tttcaatctt atagaattgc ggatcgccta tgccgatgct gtaaacggca
      421 acgttgttgc gcacggacga gtcgatcgac tcctgtcgct ttattgtgct cgcggtgtcg
      481 tcaccgtcgc ttaagagcac aatcgctcgg cgcgagtttt ccggagcttt tgccaggacc
      541 tcattcgcgc tggcccacac cgaatcccaa atgctggtcc aacagagcgg atcctcctgg
      601 ggcgttttca cattttcaca ttcggggtta tccggaggta gttcaaccct caagcgatca
      661 atagcacgtt caagttttgg gaaactgtcc gtcagatcct gctgcacggt aggagcgccc
      721 gtaaaagaaa cgatcgccac cttgtccact gctggtcgca taacagcgcc gagaaatgcc
      781 tttgccgctt ttttctcgtc gggcagagtt cgttcctgtg acttgctggt gtcgacgagg
      841 atgacaatgg tcagcggtcg gttggtttct ctttcgaaga gactgatgtc ttgaggtgtc
      901 tgattttcca gcacccgcaa atcgttcgcc cgcagcgttg tcacgaagtg gcgatcttga
      961 tcaactgccg tgaaaacggt gttcacgaga tctgtgtcaa cacgaataac ttcggcgtca
     1021 ctgtcggccg catcgacagg cggcgttggt ttggcttcag gtttagtacg cgaagtagat
     1081 tccactaatc gcggtcgttg ctgattctga ctctgagcag agctcaaggg tatctgcgtt
     1141 aacgtcaacc ccaggagagc gacgccaggg atcagaagca tctttaaagt tttgaagtgg
     1201 tagttcatct cgccttaacc cgcacagaga aaggtcagca caaagaaatg ggcagccctt
     1261 ttggagctgc ccggtcaaac tgaagtttgg tcttagtaac ggcgactgcg attacgcagc
     1321 ttgtaggtgt acaaggctga accgccacca ccggccaatg caccgatccc ggcgcccttc
     1381 ttaccaccga tcagaccgcc gatagccgcg cccgaaccgg cgccgattgc caccgtcagc
     1441 ttatcgcggt gcttatccca aaaactgcgg ctgcgcggct gcgagtagac gctgcgcgag
     1501 ttggtagagt tcgcatagct acgaccgcta ttccggtaga ccgtacgtcg cggggcatag
     1561 ctgacctgtg ccggcgttac gctacgtgca ccctcaacac ccgcctgggc agcgcggaaa
     1621 ccatcggcaa agccaacttt gtaggcaccc tgttcctcgg cggtatcaaa ggcagccggc
     1681 ataaagggat caggagttac ctggcggtcg gcaacgtaag tagcatccgt cggaactgtt
     1741 gcacgttcgg tagcagcagc cgtattggac tggtttctgg tgtgcattac cagggctcca
     1801 acggaaccgc ccacaagagc cgctatcaaa gccaccggca gtaatttgtt tgtcataatc
     1861 atcgctcaat ctccttatca aatgtctcgg cgttgaagct cgcggttaga aggagcaaca
     1921 tgcgtacctc ggaggcgtat gcagcgccag ggtttgatag taatgctaaa gcaggctggt
     1981 tttcgcggaa tactgcgcta agaaagaaag ttgcaagaaa ctcgcaataa taggtctctg
     2041 tgcctcaaca ctgttcttac ccgatatgag gaagcccgta cgcgaaatga tcagtggaca
     2101 atcacaaaaa cgaaagcggc cggggatggc ccccagccgc ttgttctgat tggtcttaac
     2161 gaactaggcc gctcgctgag tctgagcggg cttattctca cgtaacagct ctaaggcctt
     2221 cttcatttgc acgtcgtcag ccggcttggg agtgtttggt tcaggagcca catctctttt
     2281 ctcacctggc tgagcgggtt tcgctacggc gtcgtcatca ttccctgtta aatcctcggg
     2341 atcgacggac tcggccagtt ccgggccctt aacctctacc gacggcgtca ctcctgagtg
     2401 cgcccgatcc tcacccagaa aaggcttgcc acttgcgctc gcccatttta ccgtcgtgag
     2461 caacaaaccg tccccacctc ggagtgtgaa taactgttgc tctgcaccgg cgccaaaact
     2521 tttttcaccg acaatctgcc cgcgattgcg ctcgaggaga gcggatgcga caacctctgc
     2581 cgcgcccgct gtgccagtgt caatcaacac cacgacggga cctaaaaaga ctgttgcctt
     2641 gggatcggcg gtgaa
//
LOCUS       DASUEX010000122         3562 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1616258, whole genome shotgun sequence.
ACCESSION   DASUEX010000122 DASUEX010000000
VERSION     DASUEX010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3562
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1616258"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..957
                     /locus_tag="VFV61_02065"
     CDS             <1..957
                     /locus_tag="VFV61_02065"
                     /inference="COORDINATES: protein motif:HMM:NF012662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEX4899285.1"
                     /translation="KHAQQTGFDTLLIDTAGRLHIDDALMEELVSIKQETHPVEILFV
                     ADAMTGQDAVRSAEEFHRRVGITGVILTKMDGDARGGAALSIKQVIGQPVKFVGVGER
                     YDALEPFYPDRVAQRILGMGDVLSLIEEVQSKVDQKEAEDQLKKLQKNEFTLDDFRSQ
                     LRQVKKLGSFSKIMKLLPDQLLGGIGMPQLDDEQSKMMEQELRRTEAIIDSMTWEERN
                     DHRILNANRRRRISRGSGTSVAQVNQLIKQYVEMRQMMRQLSSSGMFGGGGLKSKMLR
                     KMTGMPDMAGFAGEEGQMPQIPSLPAGPSRKKLKKKKKKRRR"
     gene            1051..1257
                     /gene="rpsP"
                     /locus_tag="VFV61_02070"
     CDS             1051..1257
                     /gene="rpsP"
                     /locus_tag="VFV61_02070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEX4899286.1"
                     /translation="MKEKQSKRDGAYLENLGTYNPTRNPAEIKLNLTRVNYWIEKGAQ
                     PTDTVSRLIKASARAEQAATEAQA"
     gene            1347..1580
                     /locus_tag="VFV61_02075"
     CDS             1347..1580
                     /locus_tag="VFV61_02075"
                     /inference="COORDINATES: protein motif:HMM:NF024483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEX4899287.1"
                     /translation="MRDAIEMIVKALVDDSENVDIREVEQRNGTTLIEVRVAPNDVGK
                     IIGKQGRTIRALRSLARIGGTKKNRRYLLEIVE"
     gene            1584..2111
                     /gene="rimM"
                     /locus_tag="VFV61_02080"
     CDS             1584..2111
                     /gene="rimM"
                     /locus_tag="VFV61_02080"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEX4899288.1"
                     /translation="MEHAGASELVVIARAVRPRGLKGEIVAELLTDFPDRFEEIEELI
                     LVSPRGERTTKRLEDYWFQNDRVVLKVAGYDDVDAAKELVGFEFAVPEQDRVPLPADH
                     YYDWELEGCTVKLGEESIGTVSSVLKTGGAEILVVTDKSGKERLIPLADSIVTEVDAA
                     GKMIIIDPPEGLLDL"
     gene            2132..2833
                     /gene="trmD"
                     /locus_tag="VFV61_02085"
     CDS             2132..2833
                     /gene="trmD"
                     /locus_tag="VFV61_02085"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: protein motif:HMM:TIGR00088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEX4899289.1"
                     /translation="MLRFDIITIFPEFFHAVVDCGILRRARNAGLVEITAHDLRQWTT
                     DKHHVVDDRPFGGGDGMVLKPEPIFAGVEALTGASRREDFPAKTRVILLSAQGQVFSQ
                     ALAQEIAQNTAHAILLCGRYEGVDERVAEALVTDEISIGDYVLSGGEPAATVLIDAVV
                     RLLPGALGSDTSAVFESFSEGLLDHPQYTRPPEFRGMKVPEVLLSGNHAEIERWRKEA
                     AIAKTRRKRPDLLKE"
     gene            2858..3250
                     /gene="rplS"
                     /locus_tag="VFV61_02090"
     CDS             2858..3250
                     /gene="rplS"
                     /locus_tag="VFV61_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004415194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEX4899290.1"
                     /translation="MNRLDKIEETQLRNNIPQFQPGDTVRVHVRIKESETKERLQAFE
                     GVVIARKHGGARETITVRKTSFGVGVERIFPLHATIIDHIDLIKRGRVRRAKLYYLRG
                     LRGKAARIRERDTRGQGVDSQASAAGKK"
     gene            3268..>3562
                     /locus_tag="VFV61_02095"
     CDS             3268..>3562
                     /locus_tag="VFV61_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899291.1"
                     /translation="MRIAIDAHTVGTKLGGNESYAVNLIEALAQIDSINQYTIYITTN
                     EARERFGNHWPNFKVRSTLPHTPLIRIPLTLSAELRKNPVDVLHVQFTAPPFCP"
BASE COUNT          895 a          899 c         1047 g          721 t
ORIGIN      
        1 aaacatgcac agcaaacagg cttcgacacg ctcttgatcg acaccgcggg ccgcctgcac
       61 atcgatgacg cgctcatgga agagctcgtc agtatcaagc aggagactca tccggtcgag
      121 atactctttg ttgccgatgc gatgacgggc caggacgcag ttcgttctgc tgaagagttt
      181 caccggcgcg tcggaatcac cggagtcatc ctgaccaaga tggacggtga tgctcgcggc
      241 ggtgcagcgc tctcgatcaa acaggtcata gggcagccgg taaagtttgt cggtgttggg
      301 gaacgttacg acgcgctcga gcctttctat cccgatcgag tggcccagcg aattctgggc
      361 atgggcgacg ttctctcgct gatcgaagaa gtacagtcga aggtcgacca gaaagaagct
      421 gaagatcagc tcaagaagct tcagaagaac gagttcacct tagacgactt tcgttcgcag
      481 ttgcgacagg tcaagaagct cggttctttc tctaagataa tgaagctcct gccggaccaa
      541 ctgctcggtg gcatcggcat gcctcagttg gacgacgagc agtcgaagat gatggagcag
      601 gagctcaggc gcactgaagc catcatcgat tcgatgacgt gggaagagcg gaacgatcat
      661 cgcattttga atgcgaatcg ccgccgtcgc atctcgcgtg gctcgggaac gtcagtcgcg
      721 caagtgaatc aactcatcaa gcagtacgtc gagatgaggc agatgatgcg acagctttcc
      781 agctccggaa tgtttggcgg tggcgggctg aaaagcaaga tgttgagaaa gatgacgggc
      841 atgcccgaca tggcggggtt tgctggagaa gaaggtcaaa tgccgcaaat accttcgctg
      901 ccggccgggc catcaagaaa gaagttgaaa aagaaaaaga agaagagacg aaggtgagcc
      961 acgcgtcagt ggccctgaag gaggaagcaa ttgttagcga ttaaattaat gcgcaccgga
     1021 gctaagaaga gcccgtcata acgtgttgtt gtgaaagaga aacagtcaaa acgcgacggc
     1081 gcctatctcg agaacctcgg cacttacaat ccaacgcgaa atcccgccga gatcaaactc
     1141 aacctgactc gagttaacta ctggatcgaa aaaggtgcgc aacccactga cacggtcagc
     1201 cggttgatca aagccagcgc acgtgcggaa caagccgcga ccgaggcaca ggcctaatgc
     1261 ttgagtttag agtacagcct ttaggctgct gttttagcgt agcaagctaa agcttgaact
     1321 ctgaactcgt ttttgtgaac taagttatgc gagacgccat cgagatgatc gtcaaggccc
     1381 tggtcgacga tagcgagaat gtagacatcc gcgaagtcga gcagcgcaat ggaaccacgc
     1441 tcatcgaggt gcgcgtcgcg ccgaacgacg tcggcaagat catcggcaag caagggcgaa
     1501 cgatacgcgc gctgagatct ctggcccgga tcggcgggac caagaagaac cggcgctact
     1561 tgctggaaat cgttgaatga acgatggaac acgcgggcgc gagtgagctt gtagttatcg
     1621 cgcgagcggt tagaccccgc ggcctgaagg gcgaaatcgt cgcggagctt ttgacagatt
     1681 ttccggatcg gtttgaagaa atcgaagaac tgatcctcgt ttcgccacgt ggcgagcgaa
     1741 caacgaaacg tctcgaagat tactggtttc agaacgatcg agtcgtattg aaagtggctg
     1801 gttacgacga tgtcgacgca gccaaagagc tcgtcggatt tgaattcgct gttcccgaac
     1861 aagatcgtgt tccgctgccg gcggatcact attacgactg ggaactcgaa gggtgcaccg
     1921 tcaagcttgg cgaggagagt atcggcacag tcagctccgt gctgaaaaca ggtggcgctg
     1981 agatcctcgt tgtcaccgac aagagcggca aggagcgatt gataccgctg gctgattcga
     2041 tcgtcaccga agtcgacgct gcgggaaaga tgatcatcat cgacccgcct gaaggacttt
     2101 tggatttgta gatctttgat tgtgaagaag gatgctgcgc ttcgatatca tcaccatttt
     2161 tcccgagttc tttcacgctg ttgtcgattg tgggattttg aggcgcgcgc gtaacgctgg
     2221 tctggttgaa atcacggcgc acgatctgcg gcaatggacc acggacaagc accacgtcgt
     2281 ggacgatcgt ccttttggcg gaggcgacgg gatggttttg aaacccgagc cgatcttcgc
     2341 cggtgttgag gcgttaactg gcgcttcgcg tcgagaagac ttcccggcca aaacgcgcgt
     2401 gattttgctg tcggcccagg gccaggtctt ttcgcaagca ctcgcccagg agattgcgca
     2461 aaacacggcg cacgcgatcc tgctttgcgg gcgttatgaa ggagtcgacg aaagagtcgc
     2521 ggaggcatta gtcaccgacg agatctcgat tggcgattac gtgctgtcag gtggtgaacc
     2581 agcggcgact gtattgatcg atgccgtcgt tcgattgtta cccggcgcac tgggcagcga
     2641 tacttcggca gtgtttgaat ccttctcgga aggtttgttg gatcatccgc aatacacgcg
     2701 gccacctgaa ttcaggggaa tgaaagtgcc ggaagtattg ctgagcggca accacgcgga
     2761 aatcgagcgt tggcgcaagg aagcagcgat cgcgaaaaca aggcgtaagc gtcctgattt
     2821 gctgaaagaa taaaggattt ttaaggagca tcgagcaatg aatcgattgg ataaaattga
     2881 agagacgcaa ctgcgtaata atattccaca gtttcagcca ggcgatacgg tccgagttca
     2941 cgtccggatt aaggaatctg agaccaaaga gcgcttgcag gcttttgaag gcgtcgtgat
     3001 tgcgcgcaaa catggcggcg cacgcgagac gatcacggtg cgaaaaacga gtttcggtgt
     3061 cggcgttgaa agaatcttcc cgctgcacgc gacgatcatc gatcacatcg acctcataaa
     3121 acgcggtcgc gtccggagag caaagcttta ctacctgcgt ggattgcgcg gtaaggccgc
     3181 acgcatccgt gagcgcgaca cgcgcggaca aggcgtcgat agtcaggcgt ccgcggccgg
     3241 caagaaataa aagcttttga ggcgaagttg cggatcgcga tcgatgctca tactgtcggg
     3301 acgaagctcg ggggcaacga aagctacgcc gtaaatctca tcgaagcgct cgctcagatc
     3361 gacagtatca accaatacac gatctatatc actacaaacg aagcacgcga gcgcttcggc
     3421 aaccattggc ccaacttcaa agttcgctca acccttccgc acacaccttt aatcagaatt
     3481 ccgctgacac tcagcgccga gcttcgcaag aatcccgtgg acgtgctgca cgttcagttt
     3541 acggcgccgc cgttttgccc ct
//
LOCUS       DASUEX010000123         2558 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_826913, whole genome shotgun sequence.
ACCESSION   DASUEX010000123 DASUEX010000000
VERSION     DASUEX010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_826913"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(183..689)
                     /locus_tag="VFV61_02100"
     CDS             complement(183..689)
                     /locus_tag="VFV61_02100"
                     /inference="COORDINATES: protein motif:HMM:NF025173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HEX4899292.1"
                     /translation="MRKRVLVISGIAVLVIAAAVFATAQAGLRLNHFQGHGRSERRHG
                     PGPEMMEHMARALGLTEDQKTQIKALMDGIQATEEARHQKMEELHKQLEAVTANGQFD
                     ETKVREVANQQAQIHADQIVEHERMKAKVFSLLTPEQRVKAEEMHKRGFGEHRRRGGP
                     PEGGRPEN"
     gene            888..1592
                     /locus_tag="VFV61_02105"
     CDS             888..1592
                     /locus_tag="VFV61_02105"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX4899293.1"
                     /translation="MERILIIDDDAALCELVGEYLEPLGFKVEAVHRGDTGVERALRS
                     EHDLILLDVMLPGQNGFEVLRQIRASSKVPVLMLTARGDDVDRIVGLEIGADDYLPKP
                     FNPRELAARVRAILRRSNSRDDAEAATTLVVGDVEMELGTRLVRRSGKIVELTAVEFE
                     VLERLLRAAGRITTREELSREVLGRSPSPFDRSIDMHISNLRKKLGHQIGAAERIKTV
                     RGVGYIYAQPGVTKGA"
     gene            1599..>2558
                     /locus_tag="VFV61_02110"
     CDS             1599..>2558
                     /locus_tag="VFV61_02110"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEX4899294.1"
                     /translation="MFRSLFLKIFLWFGLAMISMVLATLAVQQFMLREPPRQPQREAA
                     DPALVESSRTAVATYERQGKAALISYMDQMEQRARMRVFLFDSELNELSGRRTPFGAR
                     DIARRVLKTNSPEFAPTEFSPLVARPVEGANETRYAFVAELPRRQPPPLIHHVMHILG
                     LILIGSAFCYWLARYLTAPVKKLRIATKELAQGNLSARVGRSGGRRDELVSLGSDFDL
                     MAEKIESLVQAQRRLLGDISHELRSPLARLNVALELARQRSGLEAASALARIQREAEN
                     LNEMIGQLLTLTRLQTGEIQKSEFDLCRLVREIADDAEFEAQSG"
BASE COUNT          532 a          729 c          705 g          592 t
ORIGIN      
        1 ccaagaaaat tgagcagtag gatgagcgga cgcttgaaat tagaggagca acctgccgta
       61 gtctctgact tcgctcgtct tgctaacctc ctcggccacc tacttctcga ttcccgaaaa
      121 gtcaaaaaca aaggcgcgac accgttgccg cgcctttgaa ttgaaagtgc caaccgaccc
      181 tgttagtttt ccggacgtcc cccttcaggc ggaccgcctc gacgacgatg ttcaccaaag
      241 ccacgcttgt gcatctcttc agctttcaca cgctgttccg gcgtcagcag actgaaaacc
      301 ttcgccttca tgcgctcatg ctcgacgatt tgatcggcat gaatctgcgc ctgctggttc
      361 gcgacttcac ggaccttagt ttcatcaaac tgcccattag cggtgaccgc ttcaagctgt
      421 ttgtgaagct cttccatttt ctgatggcgg gcttcttcgg tggcctgtat cccatccatc
      481 agcgctttga tctgggtctt ctggtcttca gtcaacccca acgcccgtgc catgtgctcc
      541 atcatttccg gtcccgggcc atgccgtctt tctgagcggc catgaccctg gaaatggtta
      601 agacgcagcc cagcctgggc cgtggcaaag actgcggcgg cgatcaccaa caccgcaatc
      661 ccacttatta cgagtactcg ttttctcatc tttgatctcc ttactttttg cttccgacca
      721 actagactag ctgctcgtgc ttacgtcaac cttatacgcg aatcaatcga gcagcgacgt
      781 aaagagttgg ttaaccaatc gtaaagtttt gtaaagacgc gacgccggag tcttacactt
      841 ctttacaaac ctctgcaccc gggtgggcta caatgccgcc ggcccatatg gaacgaatcc
      901 taatcatcga cgacgatgcg gctttatgcg aattggttgg cgaatacctg gaaccgttgg
      961 ggttcaaagt tgaggcagtc catcgcggcg acacaggcgt cgaacgcgcg ctccgttccg
     1021 aacacgatct gattttgctc gacgtaatgt tgccggggca aaacgggttc gaagtgctgc
     1081 ggcaaatccg ggcctcgtcg aaagttccgg tgctcatgct taccgcgcgc ggcgacgatg
     1141 tcgatcggat cgtgggcctc gaaatcggcg ccgacgatta cctgccgaaa ccattcaatc
     1201 cgcgcgaact ggcggcacgt gtgcgcgcga tcttgcgacg gagcaactct cgtgacgacg
     1261 cggaagcggc aaccactctc gtagttggcg acgttgaaat ggaattggga acgcgcctgg
     1321 ttcgtcgttc gggaaaaatc gttgaactga cggccgtgga gtttgaagtg ttggagaggt
     1381 tgctccgggc cgccggccgc atcactactc gcgaagagtt gtcgcgtgag gttctaggcc
     1441 gtagcccttc gccgttcgat cgcagcattg atatgcacat cagcaatctg cgcaagaaac
     1501 taggccatca aattggtgcg gcggaacgca tcaagacggt gcgtggtgtt ggttacatct
     1561 acgcccaacc tggtgtgacc aaaggagcct agcccaactt gtttcgcagt ctcttcctaa
     1621 aaatctttct ctggttcggc ttggccatga tctcaatggt gcttgccacc ttagctgtgc
     1681 agcagttcat gctgcgtgaa ccgccgcgtc agccgcaacg tgaggcggct gatccggctc
     1741 tggtagaaag ctcgcgaacg gccgtcgcca cttacgagcg ccagggaaaa gccgcgctaa
     1801 taagttacat ggatcagatg gaacaacgcg cgcggatgcg cgtcttcctg tttgatagcg
     1861 aacttaatga gttgtctggg cgccgcacgc ctttcggcgc gcgcgacata gcccgacgcg
     1921 tgctaaagac caactctccg gagtttgcgc ccaccgagtt ttcgccgctg gttgctcgtc
     1981 ccgtcgaagg cgccaacgaa acccgttatg catttgtggc ggagctgcca cgccggcaac
     2041 cgccacctct gatacatcac gtaatgcata ttctcgggct gatcctaatc ggaagtgcat
     2101 tctgttactg gctggcgcgt tatctgacgg ctccagttaa gaaattgcgc atcgctacca
     2161 aggaattggc gcaaggaaac ctttctgcgc gtgtcggtcg ctcaggtggc cgcagggatg
     2221 agttggtatc tctgggttca gattttgact taatggccga gaagatcgag tccctcgtgc
     2281 aggctcaaag gcgcttgctg ggagacatct cacatgaatt gcgatcgccg ctcgcgcgtc
     2341 tgaacgtggc gctggagttg gcacggcaac gttccggcct cgaggccgcc agtgccctcg
     2401 cccgaattca gcgcgaggct gaaaatctca atgagatgat cgggcagctc ttaacgctga
     2461 cgaggttgca gactggcgaa atccagaagt ctgaatttga tctgtgtcgc ctggtccgcg
     2521 aaatcgccga tgatgctgag tttgaggcgc agagcggc
//
LOCUS       DASUEX010000124         2117 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_25261, whole genome shotgun sequence.
ACCESSION   DASUEX010000124 DASUEX010000000
VERSION     DASUEX010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25261"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..780)
                     /locus_tag="VFV61_02115"
     CDS             complement(<1..780)
                     /locus_tag="VFV61_02115"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HEX4899295.1"
                     /translation="MKSQLVYTLDFSQINLKSLPLVGGKNASLGELFTTLKSKGIGVL
                     DGFATTADAYRSLLAQGELEYELRTLVSDFDYEDVEELRRRGHAARVAILDMPLPREL
                     RAAVINAYENLCRRLGDEPALAVRSSASAEDLPEASFAGAAETFLNVRGREALLRAVH
                     QCFASVFTDRAISYRARLGYDQLSVAISVGVQPMVRSDMGSAGVMFTLDTESGFRNVV
                     LVNSSYGLGEFVVQGVVTPDEWTVFKPTLKCGFRSIIGRQLG"
     gene            1310..>2117
                     /locus_tag="VFV61_02120"
     CDS             1310..>2117
                     /locus_tag="VFV61_02120"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX4899296.1"
                     /translation="MRSWSFVPIEAGTVAEALKQFNAELPAAILLDIDLPDGSGLDVL
                     RQIKQERPETIVIMITGNVQVETTISALRGGAYDFIGKPISLEELRITIRNGIEARQL
                     RREVEHVRKDRAREFNFRQIIGASPAMKKMLGLAAKVAESDVSSVLLQGESGTGKDLV
                     AKAIHYGSHRADHPFIAINCAALPATLIESELFGYEKGAFTDAKSRKEGLFEQAEGGT
                     LLLDEIGELELALQAKLLRVLEEGAFRRVGGLKDIALDVRVLAASNRDLKT"
BASE COUNT          508 a          569 c          591 g          449 t
ORIGIN      
        1 gccgagttgc cgcccgatga tcgatcggaa cccgcacttg agagttggtt tgaaaacggt
       61 ccactcgtca ggtgtaacca caccttgaac cacgaattca cccaagccgt aggaactgtt
      121 gaccagtacc acgttgcgaa agcccgactc agtatccagg gtgaacatga cgccagcgct
      181 acccatgtcc gagcgcacca tcggttgcac cccgacggag atcgctacgc tcaattggtc
      241 gtaaccgagc cgcgcgcggt aactgatcgc tcgatcggtg aagaccgagg cgaagcattg
      301 gtgaactgcg cgcaacagcg cttcacggcc gcgaacattc aggaatgttt cggcggcacc
      361 ggcaaacgag gcctcgggta gatcttcagc gctagccgaa gatctaaccg cgagtgcggg
      421 ttcgtctccg aggcgcctgc aaagattctc atatgcattg atcacggctg cgcgcaactc
      481 cctggggagc ggcatatcaa ggattgccac gcgagcagca tgtccgcgtc ttctgagttc
      541 ctccacatct tcgtagtcaa aatccgaaac cagggtacga agttcgtatt cgagttcgcc
      601 ttgggccagc agtgaacgat atgcatctgc ggtcgtagca aagccgtcga gcactccgat
      661 gcctttggac ttcaaagtcg tgaagagctc tcccagagat gcattcttgc cgccaaccag
      721 cggcaagctt tttaagttga tttgagagaa atcaagcgtg taaacgagtt ggcttttcat
      781 aattgaccct ttcccggaat cggattcttt gtttgcggca gcaaactaaa cgagcttgct
      841 ctaaagcttg aaagagcaac gcgtatgcct gtcagagccc agccgttgag ggtcaaattc
      901 aacgaaaaac ccgcggtttc attactgtcc ctgtcaaaaa actcgcagcg ttgcgtgaaa
      961 ctgcgcagtc tccgctagag actttccggt gggaaaaaca aaaccgccag gacgataaga
     1021 actgctcagc acgccaggaa tctgaatggt gtaagcgcga cttggttagc aattactctc
     1081 gcgctgcggc agcttctcct ttcgtcgtct tttcatcctg gcatgcctgg cgtttcttat
     1141 cgtctgggcg gtctcctttt tccaagccag cattaggccc tataatcgaa cagacctgcc
     1201 gtttagtaag tccttcaagc tgggagggca gctttaactg aaaagtagca acgagaaaat
     1261 acttgtagta gacgacgatc aggcaattcg gtggactctg agcgaagctc tgcgaagctg
     1321 gagctttgtg cccattgaag ccgggaccgt ggccgaggcg ctcaagcaat tcaacgcaga
     1381 gttaccggct gcgattctct tggacattga tctgcccgat ggttccgggt tggacgtgtt
     1441 gcgtcagata aagcaggagc gacctgagac gatcgtgatc atgatcaccg gtaacgtcca
     1501 ggttgagacc acgatctctg cactgcgtgg cggagcctac gactttattg gcaagcccat
     1561 cagcctggaa gaactgcgca tcacgatccg gaatggaatc gaagcgcgtc aactgcgccg
     1621 ggaagtagag cacgtgagaa aggaccgagc tcgagagttt aacttccggc aaatcattgg
     1681 cgcgtctccg gcgatgaaga agatgctcgg gctggccgcc aaagtggcag aaagcgacgt
     1741 gtcctcggtc ctcctgcaag gagaatcagg gacgggaaaa gacttagtgg caaaagcaat
     1801 tcattacgga tcacaccgcg ccgaccatcc cttcatcgcg atcaactgtg ccgcgctgcc
     1861 ggcaacgcta atcgaatctg agctgtttgg ttacgaaaag ggagctttca ctgatgccaa
     1921 gtcgcgcaag gaaggcctct tcgagcaagc ggaaggcggc accctgctgc tggatgaaat
     1981 cggggagctg gaactcgctc tgcaagcaaa actattgcgc gtcttggaag agggggcctt
     2041 caggcgggtc ggcggattga aagacatagc gcttgatgtg cgggtcctcg ctgcttccaa
     2101 tcgcgacctt aagaccg
//
LOCUS       DASUEX010000125         2773 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_483746, whole genome shotgun sequence.
ACCESSION   DASUEX010000125 DASUEX010000000
VERSION     DASUEX010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2773
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483746"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1830
                     /locus_tag="VFV61_02125"
     CDS             <1..1830
                     /locus_tag="VFV61_02125"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX4899297.1"
                     /translation="RQMTRLPIRRQLITLQRVLLKKLILLVLSCLLCAHTLNAQTPSA
                     YAQYLGRYTDGADYVVYFEGTRYGMTLRPALWTATQLLKQTGSNQFAVVDRTERGATF
                     TRDERGSVTGVTIRGMEGEGLRLTRAGDTPLPIEHLLQGAGRDAAEAYLAQVGVNPTR
                     ILEAAERVLARFPSKASPVVDFLATAEPKFSSNVRLHTLLGAAHIAAGNRAAGLASFR
                     RAYQLDPANKDAISGLARLQALPAKRADDKPWTLPFPLASVFKPPTPAEIHAVEADWR
                     TRDLAVRGVAEVAGGRIKFGADELSVRIVSHLVNGQKHFGAIIAPIDATPSCCPVIVD
                     VKGVSWNYFHLNLERLDSPWFMGETRRRFIYVVPSFRGEVMQFGGVSYQSEGDRTNAW
                     DGATDDAIALLNAALQTTPAADERRICVFGRSRGGSVALLFGERDPRISCVIEWAGPT
                     DWFELMGTEGWTQQELFAEGLRTRAAPSETGGQLVEHFLLKAVRGEEDLGTVRHRMLA
                     SSPLYFAKRLPRVQLHYGMEDTSVPVRNGLQLAARLKLPNSAQAAMDRGDERRSRSIS
                     RRVSRAGGSEAFFYPRQGHDTDRLMAPDLSRKFLIEALLEGRK"
     gene            1914..>2773
                     /locus_tag="VFV61_02130"
     CDS             1914..>2773
                     /locus_tag="VFV61_02130"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX4899298.1"
                     /translation="MFKRNVRARAVTLKASPLFFVVVMVLAISKFLITVAAEDWTQFR
                     GPNGSGVSASTGLPVEFGAGKNVVWKTDLPPGHSSPVLTKNRIFVTGYSKIDAPAKQN
                     AHARQASPIEKENYKLVVIALDRQTGKIVWQREVPRKITGRLQNVNNPASPSPVTDGT
                     NVYVFFQEFGLISFDAAGKERWRRPLGPFNMFYGFGASPILVDDKVILPVDQDNPSSY
                     LIAVDKNSGKVSWKIDRPVVISGYSTPIVYQPKQGPKQIVIPESFQLSAYSVVDGKRV
                     WWVRGLACEM"
BASE COUNT          584 a          755 c          804 g          630 t
ORIGIN      
        1 cggcaaatga cacgattgcc gattcgaagg cagttaatca cgttacagag agtcttgctg
       61 aagaaactta tcctcctggt tctttcctgc cttctctgcg cgcacacgct gaacgcgcag
      121 acgccttctg cttatgccca atatctcgga cgttacaccg acggcgcgga ttacgtggtt
      181 tattttgaag ggacacgata cggaatgacg ctccgcccgg cgctctggac agcgacgcaa
      241 ctgttgaaac agacagggtc aaatcagttc gcagtcgtag atcgaaccga acgcggggca
      301 acttttaccc gcgacgaacg cggaagcgtc accggagtca cgattcgtgg catggaaggt
      361 gagggactga ggcttacccg cgcgggagat acgccacttc cgatcgaaca cctgttgcaa
      421 ggcgcagggc gcgacgcggc cgaggcatat ttggcccagg ttggagttaa tcccacacga
      481 attttagaag cagccgaacg cgttctcgct cgctttcctt ccaaagcatc acccgttgtc
      541 gatttcctcg cgacggccga gccaaagttc tcttctaatg tccgactgca cacgctgctc
      601 ggcgctgccc atatcgccgc cgggaatcgc gccgctgggc tggctagttt tcggcgcgcc
      661 tatcagctcg atccagcaaa caaagatgcg atctccgggt tggctcgctt gcaggcatta
      721 ccagccaagc gtgcggacga caaaccctgg actctacctt ttccccttgc ttctgtattc
      781 aagcccccga cccctgctga aattcatgcg gtcgaggctg actggcggac acgcgatctc
      841 gcggtgcgcg gagtcgcaga agtagctggc gggcgcatca agtttggcgc cgatgagtta
      901 agtgtccgca ttgtgtctca tcttgtgaat ggccaaaaac attttggcgc gatcatagct
      961 ccgattgatg cgacaccttc ctgctgtccc gtgatcgtgg acgtgaaagg agtttcctgg
     1021 aattactttc acctcaatct ggagcggttg gattcgccgt ggtttatggg cgagacgagg
     1081 cgcaggttta tctatgtggt tccgtcgttt cgtggggaag tgatgcaatt tggtggcgtg
     1141 agttatcagt cggagggtga tcgcaccaat gcctgggacg gcgccaccga cgacgccatc
     1201 gccttactga atgccgcgct gcaaaccact ccagcggccg acgagaggcg catttgtgta
     1261 tttggacgca gtcgcggcgg gagtgtggcg ctgttattcg gcgagcgcga tccgcggatc
     1321 agttgtgtta tcgaatgggc tggcccgaca gactggtttg agctgatggg caccgagggt
     1381 tggacgcagc aggagttgtt tgcagaagga ttacgcacgc gtgccgcgcc ttccgaaact
     1441 ggcggacaac tagtcgaaca cttcttgctg aaagcagttc gcggagaaga ggatttgggc
     1501 actgtgcgcc atcgcatgct ggcgagttcg ccgctctatt tcgccaagcg actgccacgc
     1561 gtccaacttc attatgggat ggaagatacg tcggttcccg tcaggaacgg acttcagctt
     1621 gcggcccgac tcaagctccc taattcagcc caggctgcga tggatcgagg agacgagcga
     1681 cgctcgcgtt caatttctcg tcgcgtttcg cgggccggag gatctgaggc ctttttctat
     1741 cccagacagg gacacgatac cgatcggcta atggcgccgg acttgtctcg caagtttctc
     1801 atcgaggctc tgcttgaggg cagaaaatga tccgcggcgg aagtaacgtt tcctttcccg
     1861 cttttcttca actagaatcg cgctgttgaa ctccacttag caggaccaat cttatgttca
     1921 agagaaatgt tcgtgcccgc gcagtaacgc tcaaagcatc accgttgttc tttgtcgtgg
     1981 tcatggtgtt ggctatctcg aaattcttga taactgtagc cgcggaagat tggacccagt
     2041 ttcggggacc gaatgggagc ggagtatcgg cctcgaccgg actaccggtc gagttcggcg
     2101 ccgggaagaa tgtagtctgg aagacggatc tcccgcccgg acactcgtcg ccggtgctta
     2161 ccaagaatcg tatctttgtc acggggtatt cgaagattga cgcgcccgcg aaacagaacg
     2221 cgcacgccag gcaggcgagc cccatcgaaa aggagaatta caaactcgtt gtcatcgctc
     2281 tcgatcggca gacggggaaa atagtctggc agcgtgaagt gccgcgaaaa attaccggac
     2341 ggttgcaaaa cgtaaacaat ccggcttcac cttctccagt cacggacggc acaaatgttt
     2401 acgtattctt ccaggagttt ggactgatct ctttcgacgc tgccgggaaa gagcgttggc
     2461 gccgcccgct cggccctttc aacatgttct acggctttgg ggcctcgccc atccttgtcg
     2521 acgacaaagt gatcctgcct gtcgatcagg acaatcccag ctcgtaccta atcgcggtcg
     2581 ataagaacag cgggaaggta agttggaaaa ttgaccggcc cgtagtgatc tccggttatt
     2641 cgacgccgat tgtttatcaa ccgaagcagg gtccaaagca gatcgtgatt cccgaatcgt
     2701 ttcagctctc agcttattcg gtggtggatg gcaaacgtgt ctggtgggtg cgcggcctgg
     2761 cttgtgagat gaa
//
LOCUS       DASUEX010000126         3804 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_827312, whole genome shotgun sequence.
ACCESSION   DASUEX010000126 DASUEX010000000
VERSION     DASUEX010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3804
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_827312"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            123..199
                     /locus_tag="VFV61_02135"
     tRNA            123..199
                     /locus_tag="VFV61_02135"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:157..159,aa:Pro,seq:tgg)
     gene            complement(328..3003)
                     /locus_tag="VFV61_02140"
     CDS             complement(328..3003)
                     /locus_tag="VFV61_02140"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX4899299.1"
                     /translation="MTPERWHKIEQSLQAALEHDPAARAEFLERECAGDSALREELES
                     LLASVPEAERFLDKNAMEDSAALLDEEASPLPGRQIGAYSIQEKLGSGGTGEVYLAQD
                     TRLGRKVALKLLDPGFIEDSVSRTRFLREARLASSLDHSNICTIHEVGDAEGRPFIAM
                     QYVEGETLRDVIGGKPLPLDSLLSIGLQVADAIAAAHSQNIIHRDIKPGNIIVTPQGG
                     VKVLDFGLAKLLEKDAAETDTHLTVTGAIMGTPASMSPEQARGERTDHRSDIFSFGGV
                     LYEMATGQIPFKGRTRADVISALLLEAHTPAIELNKYLPPHLSTVIDRALAKDPADRY
                     QSMRELIADLRQVVAEAGGLDQLFSSSTVPRSGVPFVPTSRRTARGTFSRPVPKQIAV
                     PLLTILIVALLGLAFLTYRSWSKATLSSSQNPAPPAARLPFQSIAVLPFKPLVTRSRD
                     EALEMGMADTLINKLSSMNQIAVRPINAVRKYADLEQDAVAAGREQRVDVVLDGNIQR
                     TADKIRVTVRLIKVADGSQLWAESFDEKFTDIFAVQDRVSEKVVGLLALQLTGQEESL
                     LTKRHTDDATAYEFYLKGRYHLNRLTDDGFTKGRDYFQRAIDRDENYALAYAGLADAY
                     NRLSGYNALASTAGFPKARAAAIKALELDDKLAEAHTVLGSVNLFYDWDWSSAERELK
                     RAIEINRSYADAHQMYGYYLSSVGRLDEALDEMRRAQELDPVSLEKTAAIGEVLYYRR
                     EYDQAIGQYQKALEMDPNSGFVYWAIGRAYTEKAMYEPAIAAFQKSIPLSGDSPDEAA
                     SLGYAYARSGKLKEARQAINDLQERSQKRYVSPTVIAIIYAALGEHDQAFAWLEKAYD
                     GRDSILVFLRVEPPFDKLRSDPRFERLLQRVGLKP"
     gene            complement(3050..3622)
                     /locus_tag="VFV61_02145"
     CDS             complement(3050..3622)
                     /locus_tag="VFV61_02145"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX4899300.1"
                     /translation="MANPSAEEITGLLLAWGGGDKAALDRLIPLVHGELKRLAHRQMR
                     RERGGDTLQTTALVNEAYLRLVDYERTAPRDRSHFFAIAAQAMRRILVERARMRRSAK
                     RGSGQKVSLDEAGEASDERAAELVALDDALTSLSAIDTRKNQIVELKYFGGLTIQETA
                     EVLGISTPTVEREWHTAKIWLFREISRTRE"
BASE COUNT          810 a         1060 c         1014 g          920 t
ORIGIN      
        1 ccgtgcgaaa tcgttggcat taaccaaggc ctttttgacg gaattattga ccaccgcttg
       61 accctaagtc tgacagcctt ctagaatgcc ctgccattcg aacaaatcgt tggcgaaatt
      121 tgcggggcgt agcacagcct ggtagtgcgc tcggtttggg accgagaggt cggaggttcg
      181 aatcctctcg ccccgaccaa aacatcttaa caaaacatca ctaaaggcca ctcttgcggg
      241 tggcctttag tgtgtttgtg ctgtgaattg tgccaaacgg ctttggttta acgcgcttta
      301 gttgatcatt tcgactttgt ggttcgctca aggtttgagg ccaacacgtt gaagcaatcg
      361 ctcaaacctt ggatccgatc taagtttgtc gaacggcggc tcaactctca ggaaaacgag
      421 aatactgtcc cgtccgtcgt aagccttttc cagccatgca aacgcttgat catgctcgcc
      481 aagtgctgca tagatgatcg ctattaccgt cggcgacacg tagcgtttct gcgagcgctc
      541 ttgtagatcg tttatggctt gccgcgcctc ttttagtttg cccgatcgcg cataagcata
      601 gccgagtgag gctgcctcat ccgggctgtc gcccgataag ggtatggatt tctgaaaggc
      661 ggcgatagcc ggctcgtaca ttgctttctc ggtgtaagcg cgcccgatgg cccagtacac
      721 aaacccggag ttaggatcca tctccaacgc cttttggtat tgccctatcg cctgatcata
      781 ttctcgacgg taataaagta cttctcctat ggctgcggtt ttttccaacg aaaccggatc
      841 cagttcctgc gcacgcctca tctcatcgag cgcttcgtcg agtctcccca ccgaggataa
      901 atagtatccg tacatttggt gagcatcagc gtagctacgg ttgatctcaa tcgctcgctt
      961 tagttcgcgc tcagcgctgg accagtccca gtcatagaaa agattgaccg accccagcac
     1021 ggtgtgggcc tcggccaatt tgtcatcaag ttccagcgcc ttgatcgccg ccgcgcgtgc
     1081 tttgggaaaa ccggctgtgg acgcgagcgc gttgtagcca ctcagcctgt tgtatgcatc
     1141 ggccaagccg gcatatgcaa gggcgtagtt ttcgtccctg tcaatcgcgc gttgaaagta
     1201 atcgcggcct ttcgtgaacc catcatccgt aagtctgttt aagtgatagc ggcctttcag
     1261 gtagaactca taagctgtag cgtcatcagt atgccgctta gttaaaagag actcttcctg
     1321 acccgtcagc tgtaaggcca gcagcccgac gactttttcg gagacacgat cctgcacggc
     1381 aaagatgtca gtgaactttt cgtcaaagga ttcagcccag agttgactcc catcggcaac
     1441 tttaattaag cgcaccgtca cgcgaatctt gtcagcagtt ctttgaatgt ttccatccag
     1501 cacgacatcc actcgctgct cccgaccagc ggccacagcg tcttgctcca ggtcggcgta
     1561 cttacgcacg gcattgattg gccgcacggc gatctgattc atgctgctta acttattgat
     1621 cagtgtatct gccatgccca tctcaagcgc ttcgtcgcga ctgcgggtaa ccagcggctt
     1681 aaagggcaaa acagcgatcg attgaaacgg taagcgggcc gctggcggag ccggattttg
     1741 agaggatgac aacgtggctt tcgaccagga gcggtaagtc aagaatgcca gaccgagaag
     1801 cgcaacaatc agaatagtca ggagcggcac agctatttgc ttgggcaccg ggcggctaaa
     1861 cgtgccgcgc gcagtgcgcc ggctagttgg aacaaacgga acacctgagc gaggaaccgt
     1921 ggacgaactg aagagttgat ccaaacctcc ggcctctgcg acgacttgcc gcaaatccgc
     1981 gatcaattcc cgcattgact ggtaacgatc agcaggatcc ttggctaacg cgcgatcaat
     2041 tacagttgac aggtgtggtg gtaggtattt gttcagttca attgctggcg tgtgagcctc
     2101 aagcaacaat gcgctgatca cgtctgcccg tgttctccct ttgaaaggaa tttgcccggt
     2161 tgccatttcg tagaggacgc caccgaagga aaaaatatcg gagcggtgat cagtgcgctc
     2221 acctcgagcc tgctccggtg acatcgaggc aggggtaccc atgatcgcgc ccgttacggt
     2281 gaggtgggta tcagtctctg ccgcgtcttt ctcaagcagc ttggcaagac cgaagtcgag
     2341 gactttcacg ccgccttgag gggtaacgat gatgtttccc ggtttgatgt cgcgatggat
     2401 gatgttctga gagtgagcgg cagctatcgc atccgctact tgcaggccaa ttgaaagcag
     2461 ggaatcgagc ggaaggggct tgcccccgat cacgtcgcgc aaagtctcgc cctcgacgta
     2521 ctgcatggcg ataaagggac gaccctccgc gtctcccact tcgtggatcg tgcagatgtt
     2581 cgaatgatcc agagacgaag ccaggcgtgc ttctcgaagg aagcgtgtgc gtgaaacact
     2641 gtcctcgata aatccaggat cgagcagctt cagagcgact ttgcggccga gtctcgtgtc
     2701 ctgcgccagg tagacttcac cggttccgcc tgatccgagt ttttcctgaa tcgagtaggc
     2761 gccaatttgc ctcccaggca gtggcgaggc ttcttcatcg agcaacgcgg cggaatcctc
     2821 catggcgttc ttatcaagaa atcgttcggc ttctggcact gaggcaagca gcgactccaa
     2881 ctcctccctt aacgcgctat ctccagcgca ctcacgctcg agaaactcag cacgtgcggc
     2941 aggatcatgt tcaagcgctg cttgcagcga ctgctctatt ttgtgccaac gttcgggagt
     3001 cataaccgct gcagcaattt gcgattcagg cgccgaccct tggttgttac tattctctgg
     3061 ttcttgagat ttcccgaaac aaccatatct tagctgtgtg ccactcgcgc tccacggttg
     3121 gagttgaaat tcccagaacc tctgctgtct cctggatcgt cagtccgcca aaatatttca
     3181 attccactat ctgatttttg cgagtatcga tggctgacag gcttgtgagt gcgtcgtcga
     3241 gcgctacgag ttcggcggca cgttcatcag aggcttctcc agcctcgtca agagacactt
     3301 tctgtccgct gccacgcttc gctgagcgac gcatgcgggc gcgctccacg agaatgcgac
     3361 gcattgcctg cgccgcaatg gcaaagaaat gcgaacggtc gcgcggcgct gttctttcgt
     3421 agtctaccag ccgtagatac gcctcattaa ccagggccgt cgtttgaagt gtgtcgccgc
     3481 cgcgttcccg tcgcatctgg cgatgagcga gccgtttcag ttccccgtgg actaaaggga
     3541 tcaaccggtc gagggcggct ttgtcgccac cgccccaggc gaggagcagg ccggtgattt
     3601 cctctgcgga ggggttcgcc atttcatgcc gttggttgga gtgactcgaa acaaatcaat
     3661 tatagcatcg gtgatcgcac accttgccgt ccattctttc gacacttgac ctactcctac
     3721 tctctttaaa tctcgctgcc gccctaccaa aactcggtat cagaaaatct tttgctggtc
     3781 gtgatggaat ttgacctcgg tttg
//
LOCUS       DASUEX010000127         5463 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1056700, whole genome shotgun sequence.
ACCESSION   DASUEX010000127 DASUEX010000000
VERSION     DASUEX010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5463
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1056700"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..801)
                     /locus_tag="VFV61_02150"
     CDS             complement(<1..801)
                     /locus_tag="VFV61_02150"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEX4899301.1"
                     /translation="MITVAREHVGSEYMAWAKSSSQARFNLATSGLANVRLEELSVRL
                     EDLELTRDGGYGYQPLQNAIADRLDVHPESIVTAIGTSLANHLAMAALVKPGDEVLIE
                     QPTYEPLLALARYLGANIKRFQRRFETEFRVLPEEVERNLTSRTRLVVLTNMHNPTGV
                     LIPQETLRAVGKLAKRMGARVLVDEVYLEALFAERPPTAFALGAEFVTTSSLTKAFGL
                     SGLRCGWIVAAPELAQKIWQLNDLFGVMPAHPAERLSVIALEQLVEIGA"
     gene            complement(798..2126)
                     /locus_tag="VFV61_02155"
     CDS             complement(798..2126)
                     /locus_tag="VFV61_02155"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX4899302.1"
                     /translation="MSSDKSQAHASTQLARRLGLFDATMLVMGGIIGSGIFMNPYVVA
                     RQVSTPWLILGVWAFGGLIALVAAFIWAELAALRPDVGGQYAYLREAFHPSVAFLYGW
                     ALLLVIQSGGMAAVAVTFARYFLELTQSPISGSLVAALVLATLTAINCVGVRAGGTVQ
                     SFLMVLKIVAILALIICGLALAAPQPQEKVNATPPISIGLLTAIGAAMVPVLFSYGGW
                     QTASFVAGEIREPRRNLPRALLIGVIGVVVLYLLVNVACLYVLNPDGLAKTTTPASDV
                     MKVALGATGAKLIAAGIAISTLGFLSQGMLTAPRVYFAMAEDGLFFKSVARVHQRTRV
                     PMVAIVLQGVLAIVIALWGRYEQILNYVVSVDFIFFGLTALCLFRLRRAEQASSIRVP
                     GHPLTTFLFAAVCWIVVANTIYRYPENTLIGLAILLAGIPAYLFWKWRDG"
     gene            complement(2218..2694)
                     /locus_tag="VFV61_02160"
     CDS             complement(2218..2694)
                     /locus_tag="VFV61_02160"
                     /inference="COORDINATES: protein motif:HMM:NF025160.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
                     /protein_id="HEX4899303.1"
                     /translation="MPDKSASSVTLWSWLFPLTYLIHIGEEFYGGEGYPSYLKRLRGV
                     DMTPTRFLVGQAVGLALIVLGILIARRLNFPRQLLVILGTVVLFNGISHLATSSYYRE
                     YGPGLISGVLIWIPFGMATLIRFKDSMSRRRYWLYVAIGVGINVAIALIALKGGKR"
     gene            complement(2783..4045)
                     /locus_tag="VFV61_02165"
     CDS             complement(2783..4045)
                     /locus_tag="VFV61_02165"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX4899304.1"
                     /translation="MAETQQNLQAGTSAQPYRIDQKLHQILYKPESRPSLAPAPEEVL
                     PEPVYPQHPDPVPMSRREYQKLNAKRTWKTWGAPYFRSRWHSKELRPIIPYLFTEWKC
                     NLDCHYCWSYNNAVKGMTEDVAKRSIDWLHSIGSRVLALMGGEPLLRPKFIHKIVDYS
                     AKKDIFVYLPTNGRLMKPDVIDRLGDAGIATFNLAVDSVAEKKSLPKALNPIRPYFDY
                     LVKRQHHYGYSVMFNINICHNNMDDVKELTEIAHDNGIATDYHVNETPMLEQSHFKHL
                     DENQTYLTPDDWPKVDALLDYLQDLRMNHGYKMANPVQHMEDMKLLMRGEVPPWQCRA
                     GQNSLIIRTDGSLAPCFPMYSATHDWGTVGNHKFDVKQLDQMKQECTKHCLSTCNYIL
                     GYCYDNMRVITWGLKQAMHGFKGASNGF"
     gene            4407..>5463
                     /locus_tag="VFV61_02170"
     CDS             4407..>5463
                     /locus_tag="VFV61_02170"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX4899305.1"
                     /translation="MAAKAEAIITAGLRPELFPQRGVILGSRTLVLATVALLVFAGFS
                     FRVIGLSAEGLSEDELNKLNAVTDYRQHGLTAANGEHPFLMKALQTGSLFLAERWNTT
                     SYVAAKTQLQVSPETALRLPGTIFGALTIILIYLVAVELFGAEVALIAAALWAFDPQA
                     IGFSRVAKEDTFLLFFFLLANIFWLQGQRVAESNTSKRPQPYYWAAAASCGAMLASKY
                     LPSLLVVGLSYYWIFQAIPETRWRLGKKRMLLFLLVMSCVFLLLNPTILLPATWHEMG
                     IFAGQKRIGHDGYEFMGSLYSHRMTDWLNGTPWYFYLVFIGVKLPLLTVAGFITGLPL
                     LFRRKIGDGRYFILFWFF"
BASE COUNT         1272 a         1480 c         1468 g         1243 t
ORIGIN      
        1 cgcgccaatc tcaacgagct gctctaacgc aattacactc aacctttcag ccggatgcgc
       61 cggcatcacg ccaaagagat cgttcagttg ccagatcttt tgtgccaact cgggtgctgc
      121 aacgatccag ccacagcgca agccactcaa gccaaaagcc ttcgttaagc tgctggtagt
      181 aacgaactct gcacccagcg caaaagcagt cggcggacgt tcagcgaaaa gagcttcgag
      241 gtaaacttcg tcaacaagca ctcgcgcccc cattcgcttt gccagtttgc cgacagcgcg
      301 gagggtctcc tgtgggatta gcacgccggt gggattgtgc atgttggtta gaacaacgag
      361 gcgcgtgcgc gaagttagat tccgctcaac ttcctcgggc aacacgcgaa actcagtctc
      421 gaatcgcctc tggaagcgtt tgatgttggc gcccaggtaa cgagctagag caagcagtgg
      481 ctcgtatgtt ggttgctcaa tcaggacttc atcgcctggt ttgaccagcg ctgccatcgc
      541 caaatgattt gcgagagaag tgccaattgc cgtgacgatt gattcgggat ggacgtcgag
      601 acggtcggcg atcgcatttt ggagcggttg gtagccatag cctccatccc ttgttagttc
      661 cagatcctcg agccgaacgc tcaattcctc gaggcgaaca tttgccaggc cactcgtggc
      721 gagattaaag cgcgcctgcg agctgctttt agcccaggcc atgtattccg aaccaacgtg
      781 ttctcgtgcc accgtgatca tccgtctctc catttccaaa aaaggtaggc aggtattcct
      841 gccagcagaa tggccaaacc aatcaaagta ttttccggat aacgatagat cgtgttggcc
      901 acgacaatcc aacagacggc cgcgaatagg aatgtcgtaa gcggatgtcc gggtacgcga
      961 atgctggaag cctgctcagc gcggcgcaac ctgaaaagac acagtgctgt cagtccaaag
     1021 aagatgaaat ccaccgacac gacgtaatta agaatctgtt cataccgtcc ccacagggct
     1081 atcacgattg caagcacgcc ctggagcacg attgctacca tgggaacgcg tgtccgctga
     1141 tgcacccggg ccacactctt gaaaaacaag ccatcttcgg ccatagcgaa atagacgcgc
     1201 ggtgcggtga gcataccctg actcaggaaa cccaaagttg aaatcgcgat ccctgcggca
     1261 atcagtttcg cgccggttgc tcccagcgcc accttcatga cgtcggacgc cggcgtagtc
     1321 gtctttgcca gtccgtcagg attaagcacg tagaggcagg cgacgttcac caggaggtaa
     1381 agcaccacca ctccaatcac tccgatcaag agcgcgcgcg gcagatttcg gcgtggctcg
     1441 cgaatctccc ccgcgacaaa gctcgcagtc tgccaaccac cgtaggaaaa cagcaccggc
     1501 accattgctg cgccgattgc cgtcaaaaga cctattgaga ttgggggcgt ggcgtttacc
     1561 ttttcctgcg gttgaggagc cgccagggcc aatccacaga ttatgagggc caggatggcg
     1621 acaattttta gcaccatcag aaagctttgc actgtcccac ctgcgcgtac gccgacacag
     1681 ttgatcgcag tgagtgtagc aagaactaag gcagccacca ggcttccgct aattggtgat
     1741 tgagtcaact ccaaaaagta gcgggcgaag gtaactgcaa ctgcggccat gccgccgctc
     1801 tgaatcacga gcaacaaagc ccagccgtag agaaaggcta ctgacggatg aaaggcttcg
     1861 cgtaaatacg catactggcc gccgacgtct ggtcttaggg ccgccagctc cgcccaaata
     1921 aaggcggcga caagggcgat caatccgcca aaagcccaca cgcctagaat cagccaggga
     1981 gttgaaacct ggcgcgccac tacgtaagga ttcataaaaa tcccggagcc gatgatgcct
     2041 cccatcacca gcatcgtggc gtcaaacaga ccgagacgtc tcgctagctg agttgaagca
     2101 tgcgcctggc tcttgtctga gctcataaga atgagtgtgg aagttgggga agttagtctt
     2161 gacgcgcgca accttaccac aggatagatg cgttcacgcc gcaggtgtga atgacattta
     2221 cctcttgccg cctttaagtg ctatcaaggc aattgcgaca ttgatcccga caccaatcgc
     2281 cacgtacaac cagtaacgac gtcgcgacat actgtctttg aaacgtatga gtgtcgccat
     2341 gccaaacgga atccaaatca agacgccaga gattaagccc ggaccatatt ctcgataata
     2401 agaactggtc gcgagatggc tgatgccgtt aaagaggaca actgtgccca ggatgacgag
     2461 aagctgacgg ggaaagttaa ggcggcgggc aatcaggatt cccaggacta ttagggcgag
     2521 cccgacggct tgacccacga ggaacctcgt tggcgtcata tcaacgccgc gaagccgctt
     2581 aagatacgag ggatatcctt caccgccata gaactcttca ccgatgtgaa ttaggtaagt
     2641 cagcgggaag agccatgacc agagagtaac ggaggaggcg gatttgtcgg gcatgttcgt
     2701 tactggttat acctcggcag gttcaacaaa aaagccggag ccattccgac tccggcttct
     2761 tccatttcag ggcgacagga gactagaagc cgttgctggc acctttaaag ccatgcatgg
     2821 cctgcttcag accccaggta atcacgcgca tattgtcgta gcagtaaccg aggatgtagt
     2881 tacaagtcga gaggcaatgc ttcgtgcatt cctgcttcat ttgatcgagt tgtttcacat
     2941 caaacttatg gttgccgacc gttccccaat cgtgcgtcgc tgaatacatc gggaagcacg
     3001 gggcgaggga gccatcggtg cgaatgatta gtgagttttg accggcgcga cactgccacg
     3061 gtggcacctc accacgcatc aagagtttca tgtcttccat atgctgcacc gggtttgcca
     3121 tcttgtaccc atggttcatg cgcaagtctt gaaggtagtc gagcaacgca tcaaccttcg
     3181 gccaatcatc gggcgtcagg taagtctggt tctcatccag gtgtttgaaa tgactctgct
     3241 ccaacatcgg cgtttcattt acgtgatagt ccgtagcgat gccgttgtcg tgcgcaatct
     3301 cggtcaactc tttcacatcg tccatattgt tgtggcagat gttgatatta aacatcaccg
     3361 aataaccgta gtgatgttgc cgcttaacga gatagtcgaa gtaaggtcga atcggattca
     3421 gagccttcgg caacgacttc ttttcggcta ctgaatccac ggcgagattg aaggtcgcga
     3481 tcccggcatc acccagtcga tcaatcacgt ctggcttcat taaacgtccg ttcgtgggca
     3541 gatagacaaa aatgtccttc ttggcgctgt aatcgacaat cttgtgaatg aacttaggcc
     3601 gcagcagagg ttcgccgccc atcaaggcca gcacccgcga gccaattgaa tgcagccagt
     3661 cgatcgagcg cttggccaca tcttccgtca tgcccttaac ggcgttgttg tacgaccagc
     3721 agtaatggca atcgaggtta catttccatt cggtgaagag gtacggaatg atcggccgca
     3781 gttctttcga gtgccagcgg gaccggaagt aaggagcccc ccaggtcttc caggtccgct
     3841 tcgcgtttag cttctgatat tcccgccgcg acattggcac ggggtcaggg tgttgcggat
     3901 agactggttc cggcagaacc tcttcgggag cgggagccaa agacggtctg ctttcgggct
     3961 tataaagaat ttggtgcaac ttttgatcga tgcgatacgg ttgcgctgag gtccccgctt
     4021 gaagattctg ctgagtttct gccatttttc cccctctgag ataaaggtga tcgggcggaa
     4081 ggattaagat tccaaaacct gcggattctg gctgatgttt tagttaattg caagtgggtc
     4141 gatgtttgga cgcaggttct gctcgggtta cgccggaacg cctacccgcg gattgctgat
     4201 tgccaattgc cgattgccaa aaggtcagga gcgacaaagg ccaagcgaaa atctgcaact
     4261 ggcaatcggc cctcggcaat tcgacggcgg ggcgcgttca gttgcgagcc atgatgaatc
     4321 aggttaacct ctggcctttt gttgttgcct gaattcgcca agatgtaccg gggtgtcggg
     4381 tagggttaaa taagggagtt cttgtattgg ctgccaaagc agaagcaatc atcaccgcgg
     4441 gattgaggcc cgaactcttc ccgcagcgcg gcgtcattct gggaagccga actctggttc
     4501 tggccacggt cgccttgctg gtgttcgccg gttttagctt tcgagtgatc ggtcttagtg
     4561 ccgaaggcct gagcgaagat gaactcaaca aacttaacgc cgtcaccgac tatcgacagc
     4621 atgggctgac tgcggccaac ggtgaacacc cgttcctgat gaaggcgctg cagactggta
     4681 gcctgttcct ggctgaaagg tggaacacca cttcttacgt agctgcgaag actcaacttc
     4741 aagtctcacc tgagactgcc ttgagacttc caggcacgat cttcggagcg ctaacaatca
     4801 ttcttattta cctggtggcg gtggaactgt tcggagctga ggtcgcactt atcgccgcgg
     4861 cgctttgggc tttcgatccc caggcgattg gctttagccg cgtcgccaaa gaagacacgt
     4921 tcctgctttt cttctttctg ctcgcgaata tcttctggct gcaaggtcag cgcgtggcag
     4981 aaagcaacac gagcaaacgt ccgcaacctt actactgggc cgcggccgct tcctgcgggg
     5041 caatgttagc ttcgaagtat ctaccgtcct tacttgtcgt tggtctttct tattactgga
     5101 tcttccaggc catacctgaa actcgctggc gccttggcaa gaagcgaatg ctgctttttc
     5161 tcctagtcat gtcctgcgtg tttttgctgc tcaaccccac cattctgctg cccgcgacct
     5221 ggcacgaaat gggaatcttc gccggacaaa aacggatcgg gcacgacggc tatgaattta
     5281 tgggttcgct ctacagccat cgcatgacag attggctgaa tggaaccccc tggtactttt
     5341 atcttgtctt cataggcgtc aaactgccgt tgttgacggt tgctggcttc atcaccggat
     5401 tgccattgct gtttcgacgc aagatcggcg acggccgcta cttcattttg ttttggtttt
     5461 ttg
//
LOCUS       DASUEX010000128         4656 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1056750, whole genome shotgun sequence.
ACCESSION   DASUEX010000128 DASUEX010000000
VERSION     DASUEX010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4656
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1056750"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..921)
                     /locus_tag="VFV61_02175"
     CDS             complement(<1..921)
                     /locus_tag="VFV61_02175"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX4899306.1"
                     /translation="MAREVKKKRRDQRNSNSEKLTRRTFVKLVPALSVAAATIPEIAT
                     KSRAQNPTPTPTPVSTPSPSPTPTPAPLRVNKEMMRGAEQLFGLELTDAHKQMALQNV
                     NTNLERYETLRQIPVPLDTEPATLFHPALPGKKFNITQRKFALSKPQLPKFTTVEDLA
                     FATVTQLAELIRTRKVSPVDLTKMYLGRLKRFGPKLNCVVTLTEELALAQAAAAESEI
                     KRGKYRGPLHGIPWGAKDLFATKGIPTTWGAEPYRSQVIDYDATVVERLREAGAVLVA
                     KLSMGALAQGGRWFAGMTRNPWQVDEDRIGS"
     gene            complement(990..1583)
                     /locus_tag="VFV61_02180"
     CDS             complement(990..1583)
                     /locus_tag="VFV61_02180"
                     /EC_number="5.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase family protein"
                     /protein_id="HEX4899307.1"
                     /translation="MMKNYRQSLAFLLTGCFALTGVVAGSLFPGSVAANQTSQPKTII
                     LVRHAEKMIVPPENKDPDLSVAGEARAKEIARIFGGSGITAIYATQYKRTQQTVKPLA
                     ERLRVSVTLVEAKKTSDLVAQIRARGAGETVFIAGHNNTVPEIIAALGGPSLPIIPET
                     EFDNLYILTLQADGATKLVKLKYGSSLPVSGQSMTKP"
     gene            2067..>4656
                     /locus_tag="VFV61_02185"
     CDS             2067..>4656
                     /locus_tag="VFV61_02185"
                     /EC_number="4.1.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF008907.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional oxoglutarate
                     decarboxylase/oxoglutarate dehydrogenase thiamine
                     pyrophosphate-binding subunit/dihydrolipoyllysine-residue
                     succinyltransferase subunit"
                     /protein_id="HEX4899308.1"
                     /translation="MSQQQKPDLSEVIAENFGANATYVEGLLSRFRSNPNLVDESWRA
                     YFSELLGDGYQATQTDISDNGSRTTATVPTEAAAPPVSTAAKPAPAKKAETPTVAPAA
                     TPGIEMQPIRGAALKIVENMEASISVPTATSQRRIPVKLLDENRRFINKYLEENHRGK
                     ASYTHLIAWAMLRALDQHPRLNDGYALVDNLPTRLVRADVNLGIAIDIAKKDGTRNLL
                     VPNIKAANKLRFSEFLEAYDEVVKRAREGKLQIGDFQGTTISLTNPGTIGTVASTPRL
                     MAGQSAIIATGAIEYPAEYQAMAAEALSQLGISKAINISSTYDHRIIQGAESGSFLAR
                     VHELLVGQHNFYDDIFTDLGIPYAPLRWNVDRNPFFLSADRKREQTIKEARVMELINA
                     YRVRGHLIADIDPLHAMPLLYHPELDLETYGLTIWDLDREFITGGLAGTEIAPLREIL
                     AIVQRAYCGKVGIEYRHIQSKEQKLWIRERIRQQFVEPQPIAPEIKKKLLWKLISAEQ
                     FERFLHTKYLGQKRFSLEGCETIIPLLDRLIEDAATRGIEDITLGMAHRGRLNVLANV
                     LGHFCERIFAAFEGSAHPSFPADEGDVKYHQGAVGERETANGRTVKLTLSPNPSHLEA
                     VDPVVEGMVRAKQDAMRAQGASREEAIDRALPVLLHGDAAFAGQGIVMETLNLADLKG
                     YRTGGTIHIIINNQIGFTTSPEAGRSTIYSTDVARMTQLPIFHINGDDPEAAYRTLQI
                     ALSYRQEFNKDVVLDVVGFRKLGHNETDEPSYTQPLMYTRVKAHPGVRAIYARKLISE
                     GVIDEAELNAMIEERIKRYEDALNRAKQAVAEKEPLRAVTPVAELDGSEVIETGVAAG
                     TIKEIAQ"
BASE COUNT         1060 a         1262 c         1301 g         1033 t
ORIGIN      
        1 ggagccaatg cgatcttcat ccacctgcca cggatttctc gtcatccccg cgaaccagcg
       61 accgccttgc gccagagcgc ccatagagag ctttgcgact aacactgcgc ctgcttctct
      121 gagacgctcg acaacggtag cgtcgtagtc aatgacctgg ctgcgataag gctctgctcc
      181 ccaggtcgtc ggaattcctt tcgtcgcgaa gagatcttta gcaccccagg gaatgccgtg
      241 aagtggccca cgatacttgc cgcgcttgat ttcgctttcc gccgcagcgg cctgggcgag
      301 ggccagttct tcagtaagcg tcactacgca gttcaacttc ggaccgaaac gcttgagccg
      361 gcccaggtac atttttgtga ggtcgaccgg cgaaaccttg cgcgtgcgaa ttagttcggc
      421 aagctgcgtc acggtagcga aggcaagatc ttcgactgtc gtgaacttag gaagctgcgg
      481 cttgctcaac gcgaatttgc gctgcgtgat gttgaacttc ttgccgggta acgctggatg
      541 aaaaagtgtg gcgggctcgg tatcgagggg aaccggaatt tggcgcagcg tttcgtagcg
      601 ctcgagattc gtgttgacgt tttgcagcgc catttgtttg tgcgcatcag tcagctcaag
      661 gccgaagagt tgttcggcgc cacgcatcat ttccttattc acacgcaagg gcgccggagt
      721 cggcgtcggt gaaggcgagg gtgtggacac cggagtgggc gtgggcgttg gattttgggc
      781 gcgcgatttc gtagcaatct caggaatggt tgccgccgcg acgctgagtg cgggaaccag
      841 cttgacgaat gtacgccggg ttagtttttc actgttcgag ttgcgttgat ctcgccgctt
      901 cttttttact tcgcgcgcca ttcgctcatt ctcctttaat ctcaagatca ctggggactc
      961 acggacggac gcccccacaa acagttccct tacggtttcg tcatgctttg gccgcttacc
     1021 gggagcgaac tcccatactt aagttttacc agtttggttg ctccgtcagc ttgaagcgtg
     1081 aggatgtaga gattgtcgaa ctcggtttct ggaatgatcg gaagacttgg tccgccgaga
     1141 gcggcgatga tttccggaac ggtattgtta tggccagcta tgaaaactgt ctcgccggca
     1201 ccacgagcgc gaatctgagc cacaagatcg gaagtttttt tggcctcaac gagagtgacc
     1261 gaaactcgta agcgctcggc caaaggctta actgtttgct gggtccgttt gtactgcgtg
     1321 gcgtaaatgg ccgtgatacc gctgccacca aatatcctcg cgatctcttt cgcgcgcgct
     1381 tcgcctgcaa cactcaagtc cggatctttg ttttccgggg gaacgatcat cttctcggca
     1441 tgtcttacca gaatgatcgt tttaggctgc gaagtttgat ttgcggcaac cgagccaggg
     1501 aataagctgc ccgcaacgac gccggtcaga gcaaagcatc cagtgagaag gaaagcgaga
     1561 gactggcgat agtttttcat cataggtttg tagggtgctg gtcgaaatga agtggccgcg
     1621 taaacatatc actggcgctc acgaagtttg aagtttttgg gcggcttggg aatgccgatc
     1681 ggcgattggc gatttccaat taccgcttgg cgagtgccga ttgcggcttg ccgctttcat
     1741 tgtggtaagg cttgccttac cgagaagggg tttttgacta gcttgcgcga ggctgagcct
     1801 cgcgctgcag aaaagagcat cgggccggta aggtaaacct taccgcacgg aaggcggcaa
     1861 agccgcactc gccaatcgcc aatcgctaat cgccgatcac caatcaccaa tcaacaatca
     1921 acaatcggca atccgcaatc cgcaatccgc atgggtcgga tgcaaacgat ttccgaaggt
     1981 ggtagtctat gaagcacaat caccatcact atttcggtgt tcgcaacgcg gcagcacagc
     2041 cttaagagca gcgtgaggta gtcagtttga gtcagcagca aaaaccagat ttatctgaag
     2101 taatcgcgga gaatttcggc gccaatgcga cgtatgttga aggccttttg agccgctttc
     2161 gtagtaatcc aaacttagtc gacgaatcgt ggcgcgcata cttttccgaa ctcctcggtg
     2221 acggatatca agctacgcag acggacattt ccgacaatgg tagccggaca acggcaaccg
     2281 taccgaccga agcggcggcg ccacccgtct ccactgcagc gaagccggcc cccgcgaaaa
     2341 aagctgaaac gccaactgtc gcaccagccg caactccagg tattgaaatg cagccgattc
     2401 gcggagctgc tctcaagatc gtggaaaaca tggaggccag catctcggtc cccacggcca
     2461 cttctcagcg tcgcattccg gtgaaattgt tggatgagaa tcgccggttc attaacaagt
     2521 atctggaaga gaaccatcgc ggtaaggctt cttacacgca tttgattgcc tgggccatgc
     2581 tgcgtgctct cgatcagcat ccccgtttga acgacggtta cgcgctggtc gataacttgc
     2641 cgacgcgctt agttcgcgcg gatgtgaacc tgggaatcgc gattgacatc gccaagaagg
     2701 acggcacccg caacctcctg gtccccaata tcaaggcggc gaacaagctg cggttctcgg
     2761 agtttctgga agcgtatgac gaagtagtga agcgggctcg tgaagggaaa cttcagatcg
     2821 gagatttcca gggcactact atctcactga ctaatcccgg gacgattggc acagtagctt
     2881 cgacgccgcg actaatggcg ggtcagtcgg caattatcgc gacgggcgca attgaatatc
     2941 cggcggagta tcaagcgatg gcggctgagg ctttgtccca gttgggaatc agcaaagcca
     3001 tcaacatcag cagcacctat gatcatcgca tcattcaggg cgctgaatca ggctcattcc
     3061 tggcacgtgt ccacgagcta ctcgtggggc aacacaattt ctacgacgac atcttcaccg
     3121 atttaggcat cccttacgcg cccctgcgct ggaacgtcga tcgcaatccg tttttccttt
     3181 cagctgatcg caaacgcgaa caaacgatta aagaagcacg cgtgatggag ctgattaatg
     3241 cttatcgcgt gcgcggtcac ctgattgccg acattgatcc gctacacgcc atgcccttgc
     3301 tttatcaccc ggaactggat ctcgagactt atggtcttac catctgggat ctcgatcgcg
     3361 agtttattac cgggggactt gcgggaaccg aaattgcacc gctgcgtgag atccttgcca
     3421 ttgtgcaacg ggcctactgc ggcaaagtcg gcatcgagta ccgtcacatt caaagtaagg
     3481 aacagaaact ttggattagg gaacggattc gccagcagtt cgtagagccg caaccgattg
     3541 cgccggagat caagaaaaaa ctcttatgga aactgatttc tgcggaacag tttgagcggt
     3601 tcctgcatac caagtacctg ggccagaagc gattctcgct ggaaggctgc gagacaatca
     3661 ttcccctgct cgatcggctg attgaagacg ccgccacgcg gggcattgaa gacattacat
     3721 tgggcatggc tcaccgtggc cgcctcaatg tgctcgctaa tgtgctggga catttctgcg
     3781 aacggatctt tgccgcgttt gaaggctcag cgcatccttc attcccagcc gatgagggcg
     3841 atgttaagta ccaccagggt gcagtgggag aacgtgagac tgccaatggc cgcaccgtca
     3901 aactaaccct ctcaccgaat ccgagccatc tggaagcagt ggaccccgtt gttgaaggca
     3961 tggtgcgggc gaagcaggat gcaatgcgcg ctcagggcgc ttcgcgcgaa gaagcgatcg
     4021 accgcgcttt gccggtgttg ttgcatggcg atgcggcttt tgctggtcag ggtatcgtga
     4081 tggaaacctt gaacctcgcc gatctgaagg gctatcgcac cggcggcacc attcacatca
     4141 tcattaacaa ccaaattggc ttcaccactt caccggaggc cgggcgctcg acaatttatt
     4201 ccaccgatgt ggcgcgcatg acgcagttgc cgatctttca tatcaacggc gacgatcccg
     4261 aagctgccta tcgcacgttg cagattgccc tctcctatcg ccaagagttc aacaaagacg
     4321 tggtgcttga cgtagtcggc ttccgtaagc tcggtcacaa cgaaaccgac gagcccagct
     4381 atacgcagcc cttaatgtat acgcgcgtca aagcgcatcc cggagtgcgc gcaatctatg
     4441 cgcgcaagtt gatcagtgaa ggcgtaattg acgaagccga actgaacgcg atgatcgagg
     4501 agcgcattaa acgctacgaa gacgctctta accgtgccaa acaggcggtc gcagaaaagg
     4561 aaccgctgcg agccgtcacg ccagttgcgg aactcgacgg ctccgaagta attgaaaccg
     4621 gagtcgcagc ggggaccatc aaggagatcg ctcaaa
//
LOCUS       DASUEX010000129         2662 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_827587, whole genome shotgun sequence.
ACCESSION   DASUEX010000129 DASUEX010000000
VERSION     DASUEX010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2662
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_827587"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..878)
                     /locus_tag="VFV61_02190"
     CDS             complement(<1..878)
                     /locus_tag="VFV61_02190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CofH family radical SAM protein"
                     /protein_id="HEX4899309.1"
                     /translation="MQERTVEPVLDKALDGQRLTAEDCAALLESDDIAKIGAAADEIR
                     KRKHPDNIVTYIIDRNINYTNVCNVVCTFCAFYRRPGAPDTYVRTLDEICEKIDETIA
                     LGGTGVLMQGGLHPDFGIEWYEDLLRTLSAKYPGFQLHCFSPPEIHNIHLISGLDHET
                     IMQRLKDAGLYSLPGGGAEILDDEVRKRVATKCTTDEWLAVMRAVHKVGLRSSATMMF
                     GIGDGTVHRVRHLQRIRDLQDETGGFFAFIPWTFQRENTALGRKIKEEPTGIDYLKML
                     AVSVLFLDNIDNFQSS"
     gene            complement(936..1595)
                     /locus_tag="VFV61_02195"
     CDS             complement(936..1595)
                     /locus_tag="VFV61_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899310.1"
                     /translation="MKRLILLVLCLGFLAACSKATQQNQSAAQPQSTPKYFYFVRKID
                     GKVSPLPPDLGEFKGAQRVEGEIIGSIVQKTSGPQEKTKDGSKYIYTYFVCQGKDIEM
                     IDNAGLYHAIGTKESLLVANIEKPDVPDMEFLQVGLDPKSGGMPKLPSGGTMTHLTFE
                     TSKQAPSTLRILGEFRWDKQASQLLTTIRVKTDAGETDIPVNQLIPYRKKAEPSPSPA
                     K"
     gene            1703..>2662
                     /locus_tag="VFV61_02200"
     CDS             1703..>2662
                     /locus_tag="VFV61_02200"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX4899311.1"
                     /translation="MDAELSGSVSVRKFDLHTHFYPPIYFDKIRELPSEFSFDQSPSG
                     QTIITYRGARFFGVTPPMTDVAKRIEDMDRVGIDVEVVSLSTPNVFFADAQYQPEIAR
                     MVNDAYAELIVKHPTRFKAFASIPMDSPDEALKELHRAIDGLKMNGVILLSNVGGNAL
                     TSPQYRPFFEEANRMKLCILLHPMLPVYTEPFREYVLGPIVGFMFDTTLAVARMCFDG
                     MFRDFPDIRWIVAHLGGAVPYLMERMDNGWRDFPECRAKIDELPSTYLKRLYYDTVNF
                     NPHMLMMVRDMIGADHMVMGSDHPHLLGSIDRAVSSIEGLDITA"
BASE COUNT          554 a          748 c          723 g          637 t
ORIGIN      
        1 caggacgatt gaaaattgtc gatgttatcg aggaacagta ccgaaactgc cagcattttc
       61 aggtaatcga tgccggttgg ttcttctttg atcttgcgtc ccagcgcggt gttctcgcgc
      121 tgaaaagtcc acggaatgaa agcaaagaag ccgccggtct catcttgcag atcccgaatc
      181 cgctgcaggt gacgcacgcg atgcaccgtt ccgtcgccga tgccgaacat catcgtggcg
      241 ctcgagcgca aaccgacttt gtgcaccgcc cgcatgactg ccagccactc gtccgtggtg
      301 cacttcgtcg cgacgcgttt gcgcacttcg tcatccagta tctcagcgcc accaccgggc
      361 aggctgtaca atcccgcgtc tttcaaacgc tgcatgatgg tctcgtggtc gagacccgaa
      421 atcagatgga tgttgtgaat ctccggtggc gaaaagcagt gaagctgaaa tcccggatac
      481 ttcgcactca acgtacgcag cagatcttcg taccactcaa tgccaaagtc cggatgcagt
      541 ccgccctgca ttagaacgcc cgtgccgccc agcgcaatcg tctcgtcgat cttctcgcaa
      601 atctcatcca gcgtacgcac gtaagtgtcc ggagcgccgg gccggcgata gaacgcacag
      661 aacgtgcaga cgacgttgca cacgttcgtg tagttgatgt tccgatcgat gatgtaggtg
      721 acgatgttgt cgggatgttt gcgtttgcga atctcgtccg ctgccgcacc gatcttcgcg
      781 atatcgtcgg actcgagcag cgcggcacag tcttcagcgg tcaaacgctg gccgtccaac
      841 gccttgtcga ggacgggctc tacagttctc tcctgcatgt gctcagtcta gtgattcggc
      901 agtcaatact caaagtcgcc gaaacgattc aagcactact tcgctggcga aggcgagggc
      961 tcagccttct tgcggtacgg tataagttga ttaactggaa tgtcagtttc ccccgcgtct
     1021 gtcttaactc ggatcgtggt cagcagctgc gaagcttgct tgtcccagcg aaactctcca
     1081 aggatgcgca aagtgctggg cgcttgcttc gaagtttcga acgtcaagtg cgtcatggtg
     1141 ccgccgctcg gcagcttcgg catcccgccg ctttttggat ccagtccgac ctgcaaaaac
     1201 tccatatcgg gcacatccgg cttctcgata ttcgccacca gcagactctc cttagtaccg
     1261 atcgcgtgat acagaccggc gttgtcgatc atctcgatgt ctttgccctg gcagacaaag
     1321 tacgtgtaga tgtacttact gccgtctttt gttttctcct gcgggcccga agttttctgc
     1381 acgatcgagc cgatgatttc gccttcaacc cgctgcgcgc ctttgaattc tcccaggtcc
     1441 ggcggcagcg ggctaacttt gccatcaatt ttgcgaacaa aatagaagta cttcggcgtc
     1501 gattgcggtt gcgcagcaga ttggttttgc tgcgtagcct ttgaacaggc tgcgaggaaa
     1561 ccgagacaaa gaaccaaaag gatcagtcgc ttcatggtcg gggaaattct cctttggtga
     1621 cgctttctat caggtattga ttccactaag caagcgggcg agcgttgatc tctttagata
     1681 aggcgaaata gaatcacgcg ccgtggacgc ggagctatca ggttcagttt cagttcgcaa
     1741 gtttgatctg cacacgcact tttacccgcc catctatttc gacaagattc gcgagcttcc
     1801 ttccgagttt tcgttcgacc agagtccttc gggtcaaacg atcatcacct atcgcggggc
     1861 gcgattcttt ggggtgacgc cgccgatgac ggatgtcgca aagagaattg aagacatgga
     1921 tcgcgttggc atcgacgtcg aggttgtgtc gctgtcaacg ccgaatgtct tctttgccga
     1981 cgcacagtat cagccggaaa tcgcacgcat ggttaacgat gcctatgccg agctgatcgt
     2041 gaagcatccg acacggttca aggcattcgc ttcaattccg atggattctc ctgacgaagc
     2101 tttaaaggaa cttcatcgtg ccattgacgg gctgaaaatg aatggcgtga ttctgttgag
     2161 caatgtcggc ggcaacgccc tcacctcgcc gcagtaccgg ccgttctttg aagaagcgaa
     2221 tcggatgaag ctctgcatcc ttttgcatcc gatgttgccg gtctacaccg agccatttcg
     2281 cgaatacgtg ctcgggccga tcgtcggatt catgttcgac acgacgctgg ccgtggcgcg
     2341 catgtgcttc gacggaatgt ttcgcgactt tcccgatatt cgctggatcg tggcgcatct
     2401 cggcggcgcg gtgccttacc tgatggagcg gatggacaac ggctggcgcg actttccgga
     2461 atgccgcgcg aagatcgacg agctgccgag cacgtatctg aaacggcttt actacgacac
     2521 ggtcaatttc aacccgcaca tgctgatgat ggtgcgcgac atgatcggcg ccgatcatat
     2581 ggtgatgggc agtgaccatc cgcacctgct cggctcgatc gatcgtgcgg tctcgtcaat
     2641 tgagggcctc gatataacgg ct
//
LOCUS       DASUEX010000130         7861 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1393382, whole genome shotgun sequence.
ACCESSION   DASUEX010000130 DASUEX010000000
VERSION     DASUEX010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7861
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1393382"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            322..1335
                     /locus_tag="VFV61_02205"
     CDS             322..1335
                     /locus_tag="VFV61_02205"
                     /inference="COORDINATES: protein motif:HMM:NF024948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HEX4899312.1"
                     /translation="MSALLLLTLCFSAASAKSLAEYHLQLRESITALDTMSQHDESES
                     ESDRAARVAQTLSAVRVALPEIDHVQLGEVTVAVNNNWLHRELDSYEKTQTDRAGTLA
                     RLLERLRALEQRVAEVDQIGRTPSDKAAAGQRLAAILSRPEYTRSVQSESALARLWNR
                     LMKWLQQFIPKPKPLAPGNAGWISQLAQFLVVLLALAVLAYVIKTFAPRIFRNRPAKK
                     KSKAKPRIVLGETLAPDESAGDLLASAEALARRGELRAAIRKAYIALLVELGDRKILS
                     LAQYKTNRDYLGAVREVEPLHTNVKQLTDSFERHWYGFASASDADWANFRARYKQALQ
                     DLK"
     gene            1352..2779
                     /locus_tag="VFV61_02210"
     CDS             1352..2779
                     /locus_tag="VFV61_02210"
                     /inference="COORDINATES: protein motif:HMM:NF025617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HEX4899313.1"
                     /translation="MRQKLAIVGTILLLIGVLFAINAATYVSKDETKDFELSPNRSSY
                     HAGPTGTRALYDFLNESGSKVMRWRETPDKLLAKGNEAVRTFVIIGDTAITIDEDQAK
                     TLLLWVERGGRLVLVDRRPQVELVPRSGEWRVTTEFGDLPTLTIDPANATAMTENVKP
                     VRAAQPTVLTRDIDAVQPSKFAAGFSFVRLEKTDAGEPKSASSQPEASADEQTDSEFD
                     SEDPEPTPVPVYENAADSISPAPVVHLGESRRPLLIDYPHGRGRILLLGDPYIFANAG
                     ISLKDNLQLAINVLDARPSAESPGLIAFDEYHQGRGATRNEFVAYFTGTPVLPLLGQM
                     VLVILLILWTRGRRFARPIPLVQVDRRSALEFVASMAELQQRAGAFDLALENIYARTR
                     RVLARYAGTDYNSSRTEIASRVAARSSIEARSLEVLMRQCEGAINGEPISERQSIFLV
                     KRLREIEGELGLRMRARDIRQTQRI"
     gene            2961..3914
                     /locus_tag="VFV61_02215"
     CDS             2961..3914
                     /locus_tag="VFV61_02215"
                     /inference="COORDINATES: protein motif:HMM:NF040105.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX4899314.1"
                     /translation="MIEYISSTVAHIRNELRKVIVGQDDPIEQILIALLAEGHALVEG
                     VPGTAKTLTVKTLARIINANFGRIQFTPDLMPSDITGTNVFNISTSTFNLRQGPIFTD
                     ILLADEINRTPPKTQAALLEAMEERQVTIDGEGQQLSPLFTVLATENPIEYEGTYPLP
                     EAQLDRFLVKILLDYPSETDERQVVANWDAGFNSRRLDQVAIQPLSEADAISRCRVGV
                     SSVRMEPGVQHYAVDIVRRTRTHPFIHYGASPRASVALLLCSKALSAIRGREFVTPDD
                     VRDISRPVLRHRLSLRAEAELDGATPDAVITDILKSAEVPR"
     gene            4210..7761
                     /locus_tag="VFV61_02220"
     CDS             4210..7761
                     /locus_tag="VFV61_02220"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX4899315.1"
                     /translation="MFTRLRRTVFLFMMVIGCTSICLAQAPGNLKGTVADSAGALIPG
                     ATVEAINDATGEKHSTTTSDDGSYSISNLQVGIYTVTATASGFSSATIKEVKVSVAFT
                     TDVSLVLAAGGASANVTVTAGDAQTQLNTTDQQLSTLLDNKKIIDLPLLSRDPSALIL
                     LSPGTVSSSGIGGFIVNGQRERNNNFLVDGIDNNDADVPGIPGGIATPNIDATQEFRV
                     ITGNFNAEYGRNTGAIIAVATKNGTNDFHGNGYIYYRSDRFNARNFFDVSGAANPQQR
                     RQFGASIGGPIVHDKAFFFFNYEGDRFDQGFTVTRTVPTARARLGLFDLGGTIGTLDA
                     RETGINNALGFPIRAQQTALLNAIYPLPNRPGQGAVPGVFENFVFGSQTNDKSDSIAT
                     RVDYRMSEKHNLTGSFNFNNGNFEFCCESFPTVDDSIKSPQRTFLLALNLTSNLTPNL
                     VNEARFGGNRSELFFNGEGDPGVGTANVDAIRTAFVAQGASFPTTGFGGANGSLVGFT
                     ISGPPSIAPFDTQFRFTGTTVLADSMTWIRGDHNFKFGFEHRRVYTNGASNFGRAENL
                     NFNLPTIFGFPLLVDAGGNDLSLAGSLGTVQNFASFLYGLVGQQSQSQYFNKDGGRTE
                     ADYRGYRVREFDLFFQDTWRVRPNFTLNYGLRYEIKGVPFEVNGQMSTLVDQDPSGFT
                     PAGGFVFQLVGKNSPNPDLKLYDNDYNNFAPRFGFNWSPAWDSGFISKLTGGPGKMSI
                     RGGYGVFYDRVFGNLFSNSSANPPFQRDFVDFPGDFVEAFGRPPTQVPTAIANGFFRD
                     VNFNGDFLFPVIFALPGNNQFQSKFANPITQAWNFGFQRQIGNDMLIEADYVANKGNN
                     QLRVIDGNLTSVTRVNAIRAGIDAPRTISPTNTTNNFINGSLNSAFFQSALNLSVGQS
                     SYHSGQFRLTKRLTNKSFGLGQVQVAYTYSHSIDDSTDPLVGQTGERTFPRDSSGFAG
                     GWTAERGNSGFDTRHRFVANYIYEFPLYFENKALDYLFGHWSMGGILQVQSGNPYSVF
                     GGTDSAGTALGQRADFADPNAPVGTFTRLAATTGLDARTQTGPTRDLFRNPCAGVVSA
                     TGASCTSQFIGRQGNVARGAFVGPSFNKFDFNLVKQVPLDHFREGMKLTLRADFFNLF
                     NRVNFGQPVNTINSANFGQSTTAAAGRIVQFVGRFEF"
BASE COUNT         1766 a         2209 c         2064 g         1822 t
ORIGIN      
        1 ctcggcccga tgccggacat caacgttacc tcgcctttcg ctcccgcgat tgtgcagagt
       61 cccggagcta ctccaccccc acgcttcgcc tcgccgggaa gcgtgctcgg actgagatga
      121 gttgctgaag tgcgccaact acttcatctt cattttctta ctcgccgatt ggattgcgcg
      181 aatcgatgcg gtacggaagc ggtagcgacg gcattcaacg aaactggctt tgaggttcgg
      241 acgcagagtg gctgctcagt tgagtgcaat cgctaccact cccgtgctgt accggtcttt
      301 tcgtgcgccg caatacgcct cttgtcagcc ctgttgttac tgacactgtg cttctccgcc
      361 gcgagcgcca aatcgctagc tgagtatcac ctgcagttgc gtgaatcaat caccgcgctc
      421 gacaccatga gccagcatga tgaaagcgaa tctgagagtg accgagcggc gcgagtagcc
      481 caaactttga gcgctgtgcg ggtagccttg ccggagattg accatgtcca actcggtgaa
      541 gtcacggttg ccgtcaacaa taattggctg catcgcgagc tcgacagcta cgagaaaact
      601 cagacggatc gcgccggaac actcgcgcgc cttctggaac gtttgcgggc gctggaacag
      661 cgcgttgccg aagttgatca gatcggcagg acgcccagcg acaaagcagc agccggccag
      721 cgcctggcag cgattctgag ccggcctgaa tacacgcgca gtgtgcaaag cgagagtgcc
      781 ctggcgcgac tttggaaccg actcatgaaa tggttgcagc agttcattcc gaagccgaag
      841 ccattggcgc cgggaaacgc cggctggatc agccagttgg cccagttcct cgtagtgctg
      901 ctggcgctcg cggttctagc ctacgtcata aaaacctttg cgccgcgcat cttcaggaac
      961 cggcccgcga agaagaagtc caaggcgaag ccgcgcatag tcttaggcga gacgctggct
     1021 cctgatgaat cggccggcga tctactcgcg agtgccgaag cgctcgcccg gcgcggggaa
     1081 cttcgtgctg ccattcgcaa agcctacatt gccttactgg tcgaacttgg tgaccgtaag
     1141 attctgagtc tggcgcaata caaaaccaat cgcgattacc tcggcgccgt gcgcgaagtt
     1201 gaaccgctgc acacaaacgt caaacagttg actgacagct tcgaacgaca ctggtacggc
     1261 tttgcctcag cctccgatgc cgactgggca aactttcgcg cgcgctataa gcaagcgctc
     1321 caggatctaa agtaagccaa acaactcacc aatgcggcaa aaactcgcca tcgtcggaac
     1381 tatcctgctg ctgatcggag ttctgtttgc gatcaacgcc gccacttatg tcagtaaaga
     1441 tgagacaaag gatttcgagt tgtcgcccaa tcgttcctct tatcacgcag gtcccaccgg
     1501 cacgcgcgcg ctttacgact ttctcaacga atccggaagc aaggtgatgc gctggcgcga
     1561 aacgccagac aagctgctgg ctaaaggcaa cgaagccgtg cgcacttttg tgatcatcgg
     1621 cgacactgcg ataacgatcg acgaagatca ggcaaaaacg ctgctcctct gggtcgagcg
     1681 tggcggaaga ttggttttgg tcgaccgacg accgcaggtt gagttagtac ctcgttccgg
     1741 cgaatggcgg gtaacaaccg agttcggtga tttgccaacc ttaacgattg accctgccaa
     1801 tgcaacagct atgactgaga acgtcaaacc agtgcgagcc gcgcagccta ccgtactaac
     1861 gagagatatt gatgcggtgc aaccttcgaa atttgcagcc ggttttagtt ttgtgcgcct
     1921 ggaaaaaacc gacgctggcg aaccaaagtc tgcgtccagc caacctgagg cctctgcgga
     1981 tgagcaaacc gactctgaat tcgattccga agaccctgag cccacgcctg tgcctgttta
     2041 cgaaaatgcg gcggatagta tttccccggc gcccgttgtg cacctggggg agtcgcgccg
     2101 tcctttactc atagactatc ctcacggccg cggccgcatc ctcctgctcg gcgacccgta
     2161 cattttcgca aacgccggaa tcagtttgaa agataacctg cagttggcga tcaacgtgct
     2221 tgatgcccgg cccagtgctg aaagccctgg cctgattgcc ttcgacgaat atcatcaggg
     2281 tcgcggtgca acgcgcaatg agttcgtcgc ctacttcacc ggaacaccag tgttgccact
     2341 gctggggcaa atggtcctcg tgatccttct gattctttgg acgcgcggac gacgcttcgc
     2401 ccgacccata ccgttggttc aggtcgatag gcgttcggcc ctggagtttg tggcctcgat
     2461 ggccgagctg caacagcgtg ccggcgcttt cgatttagcg ctggaaaata tttacgcgcg
     2521 gacgcggcgg gtcctggcgc gttacgcggg cactgactac aacagttcgc gaaccgagat
     2581 tgcgtcgcgc gttgcggcgc ggtcatccat tgaagcgcgc tctttggagg tgttgatgcg
     2641 acagtgtgaa ggagcaatta acggcgaacc gatcagcgaa cgccaatcga tctttcttgt
     2701 gaaacggtta cgtgagattg aaggtgagct tggtctgcgt atgcgggctc gggatatcag
     2761 acaaacgcaa cggatctaaa acgagctttg tactttgaac tttgtacttt gtgcttcctg
     2821 cttagtgctt tgttcttctg gccgcgcttg agctgctgga aggtatcatt cagctttggt
     2881 aagcgaacaa aacacaaagc actaagcacc aagttcaaac taattcaaga ttcgattctt
     2941 aattcaacct aggacaccag atgattgaat acatttcctc caccgtcgct cacattcgca
     3001 acgaactgcg caaggttatc gtcggccagg acgatccaat cgagcagatt ctgatcgcgc
     3061 tgctcgccga aggtcacgcg ctcgttgaag gtgtgccggg caccgcgaag accctcacgg
     3121 tcaaaacact cgcgcgcatc atcaacgcta actttggccg catccaattc acgcccgatc
     3181 taatgccgtc tgacattacc ggcacgaatg tcttcaacat ttcgacgtcc accttcaatc
     3241 tgcgacaggg tccaattttt accgacattc tcttggcaga cgagatcaat cgaaccccgc
     3301 caaaaactca ggcggcctta cttgaggcaa tggaggagcg ccaggtgacg atcgatggcg
     3361 aggggcaaca actctcccca ctcttcacag tgcttgcgac tgagaatcca attgagtatg
     3421 aaggcaccta tccccttccc gaagcgcagc ttgatcgttt tctagtaaag attttgctgg
     3481 attaccccag cgaaaccgac gagcgacaag tggtagctaa ttgggatgcc ggattcaatt
     3541 cgcgtcgtct ggatcaagtt gcaattcaac ctttgtctga ggccgacgca atttcacgtt
     3601 gccgcgtcgg cgtcagttcg gtgcgcatgg aaccgggtgt ccaacactat gccgtggaca
     3661 ttgtgcggcg cacacgcacg caccccttca tccattacgg cgcgagtccg cgcgcgtcag
     3721 tcgctttgct gctgtgttca aaggcgctgt ccgcgatccg cggtcgcgag tttgtgactc
     3781 ccgatgacgt cagagatatt tcgcggccgg tcttgcgtca tcgcttgtcg ttgcgcgctg
     3841 aagctgaact cgacggcgcc acgcccgacg cggtgattac agacattctc aagtcggctg
     3901 aagttcccag gtagtgatcc tcaccgggtc tgcttcgctg aaattctgta tttttgtcca
     3961 taagctcgag cgagggcgta gtccgtcaaa cgctcgttcg cgaccaacgc ttaatccata
     4021 aaattgaaag caatagtttc gtttcttgga tagccaagac cagtcgcgat ctgaacattt
     4081 gaatttgact gaaaattgtc gcctgcggtt gtcggtatag tcgttgccta cttaagtctt
     4141 catcccccca gaacaaattc tccccacaat aaacttccca ggaagctacg aaatttcaag
     4201 gagtaatcca tgtttacaag attacggcga accgtgtttc tttttatgat ggtgattggt
     4261 tgcacaagta tctgcctggc gcaggctccg ggaaatctca aagggacagt tgcagactct
     4321 gcgggtgctt tgattccagg tgcaacagtg gaagcgatca atgacgccac gggcgaaaaa
     4381 cactctacca ccacttcgga tgatggaagt tacagcattt ccaaccttca ggttggcatc
     4441 tataccgtta ctgccaccgc ttcagggttt tcgtccgcga ctatcaaaga agtgaaagtt
     4501 tccgtcgcct tcacgaccga cgtttcgctc gtgcttgcag cggggggcgc ttccgcaaac
     4561 gtgacggtaa ccgccgggga tgctcaaacc cagctcaaca caaccgatca gcaactctct
     4621 acactgctgg acaacaagaa gattatcgat cttccgcttt tgtcgcgcga tcccagcgca
     4681 ctgatcctgc tatcgccggg cactgttagc tcaagcggta tcggcggatt catcgttaac
     4741 ggtcagcgcg agaggaacaa caattttctc gtcgatggta tagacaataa cgatgccgat
     4801 gtcccgggaa tccccggagg catcgccaca cctaacattg atgcgacgca agaatttcgc
     4861 gtcatcaccg gcaatttcaa cgccgagtat ggacgcaaca ccggcgccat catcgccgta
     4921 gctacgaaaa atgggaccaa cgactttcac ggcaacggtt acatttatta tcgttccgac
     4981 cgatttaacg cccgcaactt ctttgacgta tccggggcgg cgaatccgca acagcgcaga
     5041 cagtttggcg cttcaatcgg cggcccaatc gtccacgaca aagctttctt cttcttcaac
     5101 tacgaaggag atcgcttcga ccaggggttc acagtgactc gcacggttcc gaccgcgcgg
     5161 gccaggctgg gtctttttga tcttggcggc acaatcggca cgctggatgc ccgggaaacc
     5221 ggaatcaaca atgccctggg cttcccgata cgcgctcagc agaccgcgtt gctaaatgcc
     5281 atttatccgc tgccaaaccg tcccggccag ggagcggttc ctggtgtgtt cgaaaacttt
     5341 gttttcgggt ctcagactaa tgacaagtca gacagcattg ccacacgtgt cgactaccgg
     5401 atgagcgaga aacataactt gaccggtagc ttcaacttta ataatggcaa cttcgaattt
     5461 tgctgtgaat cgttcccgac cgttgacgac tcaatcaaga gtccgcaacg aacttttttg
     5521 ctggcgctga atctcacctc caacttaact cccaatctcg ttaatgaggc gcgcttcggt
     5581 ggaaaccgtt cggaactctt ctttaacggt gaaggtgatc ctggagttgg gacggctaac
     5641 gtcgatgcga tcagaacagc ctttgttgcg cagggcgcta gttttccgac gaccggcttc
     5701 ggtggagcca atggatccct ggtcggattc acaatctccg gaccgccgag tatcgccccg
     5761 tttgataccc aattccgctt taccggcacg acagtccttg ccgactcaat gacctggatt
     5821 agaggtgacc ataacttcaa gtttgggttt gagcaccgca gggtttatac gaacggcgcc
     5881 agcaactttg gccgcgctga aaaccttaac ttcaatctgc cgacgatctt tggcttcccg
     5941 cttctggtcg atgccggtgg aaacgatctt agtcttgccg gttcgctggg aacagtgcag
     6001 aacttcgcca gcttcctcta cggcttagtg ggtcagcaat cccagtcgca gtatttcaat
     6061 aaggatggcg gccgcactga agccgactat cggggatacc gagttcgtga attcgatctc
     6121 ttcttccagg acacctggcg ggttcgtcca aacttcacgc tgaactacgg cctgcgttac
     6181 gagatcaagg gtgtgccctt tgaagtcaac ggtcagatgt ccacgctcgt cgatcaagac
     6241 ccaagcgggt ttactcccgc aggtggcttc gtgtttcaac tggtgggaaa gaactctccg
     6301 aatcctgatc tgaagctcta tgacaacgac tacaacaact ttgctccccg cttcggtttt
     6361 aactggagtc cagcatggga cagcggattc atatccaagt tgaccggtgg cccgggcaag
     6421 atgtcgatca gaggtggtta cggggttttc tatgatcgcg tcttcggtaa cctgttcagc
     6481 aactccagcg ccaacccgcc gttccaaaga gacttcgttg atttcccggg cgacttcgtt
     6541 gaggcttttg ggcgcccgcc aactcaggtt cccacagcga ttgctaatgg gttcttcagg
     6601 gatgtcaatt tcaacggcga tttccttttc cctgtaatct ttgctctccc aggtaacaat
     6661 cagtttcaga gcaagtttgc taatccaatt acccaagcct ggaactttgg gttccaacgc
     6721 caaatcggaa atgacatgtt gattgaggct gattatgtag ccaataaggg aaacaatcag
     6781 ttgcgcgtaa tcgatggcaa cctgaccagc gtgacacgcg ttaatgctat ccgagcgggg
     6841 atagacgcgc ctcgaacgat tagcccaacc aacacaacta acaacttcat taacggctcg
     6901 ctgaattcag ccttctttca gtcggcgttg aatctgtctg tcggccagtc aagctatcat
     6961 tctggccaat tcagactcac caagaggttg acgaataaat cgtttggcct cggtcaggtt
     7021 caagtggctt acacgtacag ccattccatc gatgactcga cagacccatt ggtcggtcag
     7081 acgggtgagc gcaccttccc gcgcgactcg tcagggtttg ctggtgggtg gacggcggaa
     7141 cggggtaact caggatttga tactcgtcac cgctttgtgg cgaattacat ctacgagttc
     7201 ccgctctatt ttgagaacaa agccctggat tacctattcg gacactggtc gatgggcgga
     7261 attcttcagg tccagtctgg caatccctac agtgtctttg gcggtactga tagcgcggga
     7321 actgcccttg ggcaacgggc cgattttgcc gatcccaatg ctcccgttgg aaccttcacc
     7381 cgccttgccg cgactactgg tctcgatgca cggacacaaa cgggtccaac gcgggacttg
     7441 tttagaaacc cctgcgctgg agtcgtgagt gcgaccggag cttcctgcac cagtcaattc
     7501 attggcaggc agggtaacgt cgctcgcggg gccttcgtcg gaccgagctt caacaagttc
     7561 gacttcaacc tggtgaaaca ggttccgctg gatcatttcc gcgaaggtat gaagctcacg
     7621 ctgcgcgcgg atttctttaa cctgtttaat cgcgtaaatt ttggccagcc agtgaacaca
     7681 atcaacagtg caaactttgg ccaatcaact acggcggcag ctggaaggat cgtccagttt
     7741 gtcgggcgct tcgagttcta agctcggaac ttggtagttt tactcgactg gcgttgcttc
     7801 ccgcgaagca gcgccagttt tttctaaaca tgcagcttcg attcagcgaa tgaaattatc
     7861 g
//
LOCUS       DASUEX010000131         3752 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_598882, whole genome shotgun sequence.
ACCESSION   DASUEX010000131 DASUEX010000000
VERSION     DASUEX010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3752
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_598882"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1664)
                     /locus_tag="VFV61_02225"
     CDS             complement(<1..1664)
                     /locus_tag="VFV61_02225"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX4899316.1"
                     /translation="MSVDKDLVSVQQARDLIEAAHKAQAEVARFDQAKIDRICEAMAK
                     VAMREAARLGAMAVEETGYGVPADKQEKNRFAAEDVWNYFRGLRTVGVVSESKDVVEI
                     ASPRGVVAGIIPSTNPTSTAIFKILIAIKSRNAIVLSPHPSAARCINETVRVMREAGI
                     KEGLPADAIGCMTTATIEGTETLMKHKQTAVILATGGIGLVRAAYSSGKPAFGVGPGN
                     VPAFIERTADVPKAVQDILTGKCFDNGTICASEQAVICDAPIEKAVREQFKAQGAHFL
                     SASEADQLGRIVATPQRSLNPGIVGRSVEVIAKMAGLSVPPGTRCLLADVAGVGRDFP
                     LSMEKLSPILAFYVVDGVEQGAARSREVLSYGGMGHTAGMHTRSREAAVRVGAELPAS
                     RITINTPTTHGAIGFSTALPPSMTLGCGSWGGNVTSDNVSPLHLMDIKRIAFETRPVK
                     SARPAASRAASSPMPVAAAPQPKQPSVAVAGGKINREEIAAIVDKFLAGRSPAPADVP
                     RPAPSQAVEVEAGSMYSGPPASGRTTAPAPPPAAPVAADRTASDGRT"
     gene            complement(1938..2204)
                     /locus_tag="VFV61_02230"
                     /pseudo
     CDS             complement(1938..2204)
                     /locus_tag="VFV61_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183618.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
     gene            2673..3200
                     /locus_tag="VFV61_02235"
     CDS             2673..3200
                     /locus_tag="VFV61_02235"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX4899317.1"
                     /translation="MAATIGEQLRLAREERGIPLREISDETRISIRYLEAIESGDFKR
                     LPGGIFNRSFVKAYARCIGYDEKKAVEAYSRSMLDLGEGGDEVVTTPYQSKVYTDVPA
                     TRSPVLTVALAIIILALLTLGALAALHWFQRRSAQFNQAPASLLALYQIANGDEVVVD
                     AYLESRKAGNEAVQQ"
     gene            3356..>3752
                     /locus_tag="VFV61_02240"
     CDS             3356..>3752
                     /locus_tag="VFV61_02240"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEX4899318.1"
                     /translation="MVPTTIPEGLTFDDVLLVPARSDVLPSETDTSTQFTRNIRLQIP
                     LCSAAMDTVTEAALAIALAQQGGIGVIHKNLSVSRQAEEVDKVKRSESGMIVDPVTIR
                     PDRPVREALMVMENYHISGVPVVDEDGHLV"
BASE COUNT          806 a         1081 c         1003 g          862 t
ORIGIN      
        1 gtgcgcccat cactcgcagt cctgtcagca gcaacgggag cggcgggagg tggcgcaggt
       61 gcagttgttc ttccgctcgc cggtggcccg gaatacatac ttcccgcttc cacttccact
      121 gcctgcgatg gggccggacg cggcacatcc gccggtgctg gcgacctgcc cgccaggaac
      181 ttatcgacaa tcgcagctat ttcttcgcgg ttgatcttgc cgccggcgac ggccactgaa
      241 ggctgtttgg gctgtggcgc ggcggcgaca ggcatcgggc ttgatgctgc cctggaggca
      301 gccggtcgcg cactcttcac cggtctggtt tcaaaggcga tacgcttgat atccatcagg
      361 tgaaggggcg acacgttgtc tgaggtaacg tttccacccc acgaaccgca gcctaaggtc
      421 atcgacggcg gcagggccgt agaaaatcca atcgcgccat gcgtggtggg tgtattgatc
      481 gtgatgcgcg acgccggtag ctctgcgcca acgcgaaccg cggcctcgcg cgatcgcgta
      541 tgcattccgg cggtatggcc catacccccg taactgagaa cttcgcggct gcgcgctgct
      601 ccttgttcca caccatcgac tacgtaaaac gcgagaatcg gcgagagctt ctccatcgac
      661 aaaggaaaat cgcgaccgac tccagcaacg tcagccagca aacaacgagt gcccggcggc
      721 acactaagcc ccgccatttt ggcaatcact tcgaccgagc ggccgacaat tcccgggttg
      781 agtgagcgct gcggcgtcgc gacgattctt cctaattggt cagcttcgga agcggagaga
      841 aaatgagccc cttgtgcttt gaattgttcg cgaaccgcct tctcaatcgg ggcgtcgcag
      901 atcactgctt gctccgaggc gcagatcgtt ccgttgtcga aacactttcc cgtcaagata
      961 tcctgaaccg ctttgggcac atccgcagtt ctctcaataa acgccggcac gttaccgggt
     1021 ccaactccga aggccggttt gcctgacgag taagcagcgc gcaccaaacc gatgcccccc
     1081 gtcgccagaa tcacagcagt ctgcttatgc ttcatgaggg tttcggtacc ctcaatcgta
     1141 gccgtggtca tgcagccaat cgcatctgcg ggcagtccct ctttgatccc cgcttcgcgc
     1201 atcacccgaa ctgtctcgtt gatacaacgt gcggctgatg gatgtggtga aagcacgatt
     1261 gcgtttcgcg atttgatggc gataaggatt ttgaaaattg cggtcgaggt aggattggtt
     1321 gaggggatga tacccgcgac cacgccgcgt gggctggcga tctccacaac gtctttggat
     1381 tcagagacga caccaaccgt gcgtaatccg cgaaagtagt tccaaacgtc ttcagccgcg
     1441 aagcgattct tttcctgctt gtccgcggga acaccataac cggtctcctc gacggccatg
     1501 gcacccaggc gcgctgcttc gcgcatcgcg actttagcca tcgcttcgca gatgcgatcg
     1561 atcttagctt ggtcaaaacg ggcaacttcg gcctgcgctt tatgagcggc ttctataaga
     1621 tcgcgcgctt gttgcaccga tacgagatct ttgtctaccg acatcgccaa ccccccaacg
     1681 attgccaatt gccaattgcc aattttcgat tggtgcgaat tttaagaagg tctttccaaa
     1741 aatcagcggt tcgccctgat acgcattttt aatcggcaat cagcaattga aaatcgccaa
     1801 tcgaaaacct atctggcttt ttctttattc gcgtcacgcg tacccgctgg tagcgatctg
     1861 tcaccttgcc ccagttggcc gcgcgctccg ccttgcaatg aacgctcatc cggactcaat
     1921 cctgtctgac catcaaccgg gagaactcgt tcaacttctg catggggtcg gggaattacg
     1981 tgaacactga tcaactcgcc tacgcgacga gcggcggccg cgccggcatc cgtggcggcc
     2041 ttgaccgcgc ctacatcacc gcgcacaaaa atggtcacgt agccagcgcc gatatactct
     2101 tttccgagta gttgcacatt cgcagctttg accattgcat ccgcagcctc aacggctccg
     2161 acgaagcctt tcgtttcgac cattccgagt gcttctaagg gcatctctcg tctcctcact
     2221 tttttccgtt gtagaaatta aagctgattt tcaggtagct ggcgccaacg taacaccgca
     2281 gacacgaaga atcaagagag gttgataagc gtaatcgtga atcgtgaatc gtgaatcgtg
     2341 aattgtgcgt gacaaacgct ggtttcggga tggcgcactt ccaaatgaca ctagaaacgt
     2401 gagattcgag tgaattgccc gatccgcgat ctccgatccc cggattcacg atccccgtat
     2461 tcccgattcc cgattcacga ttcacgaagt ttgacaccgt ttagcatcgc aagctatatt
     2521 gcggccaaga gtaagtcact cttagagata aggttccatc gaggttttat gacaacctgc
     2581 actcgcagga attagggtga agtctaattt ccacgggctc tgctaggttt gcagaccaaa
     2641 caaactatcg cagggactga cgcggattgg taatggcggc tactatcggc gaacagttgc
     2701 gactcgcacg cgaggagcgg ggcattccac tccgagagat ttccgacgaa acccgcatct
     2761 ctatccgtta tctcgaagca atcgaatcag gcgatttcaa acgactgccc ggtggaattt
     2821 tcaaccggag tttcgttaaa gcctatgccc gttgcatcgg ctacgacgaa aagaaggcag
     2881 ttgaggctta ctctcgatct atgctggatc tgggcgaggg cggcgacgag gttgttacca
     2941 caccctatca gtcgaaggtc tacacagatg ttcccgccac gcgttcgccg gttttgacgg
     3001 tggcgctggc gataattatt ctggctctat taactcttgg tgcgcttgcc gcgttacact
     3061 ggttccaacg tcgctcagct caattcaacc aggccccagc ttcgctgctg gcgctgtatc
     3121 agatagcgaa tggcgacgaa gttgttgttg atgcgtatct tgagtctagg aaagctggca
     3181 acgaagcagt tcagcaatag tttttgcgtt gaccgctggc ttgcgcctga cggcgcggcc
     3241 gaggtgctgc gcgcaatttg tttggaggta gctgcccgct cctgaaagcg ggcagaactg
     3301 ttttcgggat gtctcaatct caaatcattt tgctacggga gtaattatca aaatcatggt
     3361 tccgactacg attcctgaag gcctgacatt cgacgatgtg cttctggttc ccgcacgttc
     3421 tgacgtgttg ccgtcagaga ccgacacgtc cactcaattt acccggaata tccgtttaca
     3481 aattccactc tgctcggcgg cgatggatac cgttaccgaa gccgccctcg caatcgccct
     3541 cgcccaacag ggtggcatcg gcgtgattca caagaacctc agcgtttcgc gacaggccga
     3601 agaagtcgac aaggtaaaac gatctgaatc gggaatgatt gtcgatcccg tcaccatccg
     3661 acccgatcga ccggttcgcg aagccttaat ggtgatggag aactatcata tcagcggcgt
     3721 cccggtggtc gatgaggacg gtcacctggt gg
//
LOCUS       DASUEX010000132         2340 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_26296, whole genome shotgun sequence.
ACCESSION   DASUEX010000132 DASUEX010000000
VERSION     DASUEX010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26296"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..822)
                     /locus_tag="VFV61_02245"
     CDS             complement(<1..822)
                     /locus_tag="VFV61_02245"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEX4899319.1"
                     /translation="MADVIVEARAVELEHPPLPQVESCVLVVFGASGDLTRRKLMPAL
                     FHLACEGCMREFQILGVGRNEMTHEEFRRMMREGARELGKLEVGDDQWNEFAQRLHYI
                     AGDFAGNEVFHKIAALSEELASHGAGWNRLYYLATPPSLAPTVVEGLGEAGLADQEKG
                     WTRIVFEKPFGRDLESARTLNDQIAKVFTEDQVYRIDHYLGKGTVQNILVFRFGNSMF
                     EPIWNRNFVDHVEITAAEELGVGSRAGYYEEAGALRDMVANHLLQLLTLTAMEPTV"
     gene            complement(826..1836)
                     /gene="gnd"
                     /locus_tag="VFV61_02250"
     CDS             complement(826..1836)
                     /gene="gnd"
                     /locus_tag="VFV61_02250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HEX4899320.1"
                     /translation="MQIGMIGLGRMGANMVKRLLRGGHQCTVYDRDAVAVETLVREGA
                     LGASTPSEFISKLDPPRAIWLMIPAAVVDKTLQDLLLAAESGDIIIDGGNSYYVDDIR
                     RAKELKARGIHYVDVGTSGGIWGGERGYCQMIGGEGEVVRHLDPIFKTLAPGRGSAPR
                     TPGRDNNASTAEEGYLHCGPSGAGHFVKMVHNGIEYGLMEAYAEGLNILFHANAGKQD
                     RATDAETSPLRNPEHFQYDFNLAEITELWRRGSVVASWLLDLTAIALLEQPHLEEFSG
                     RVSDSGEGRWTINAAVEESVPVPVLSAALFERFTSRGEADFANKVLSAMRFQFGGHAE
                     KK"
     gene            complement(1883..>2340)
                     /locus_tag="VFV61_02255"
     CDS             complement(1883..>2340)
                     /locus_tag="VFV61_02255"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX4899321.1"
                     /translation="FSQAMIDRAKVRLGETGQSNVSFAVADITSRWPCDDESYDLVTC
                     SLVLEHIKEIDFIFAESSRCLRDGGRLFLCELHPYRQYAGTKANFTRDQKVTQIDSFV
                     HHVSDFLDGTARNGLSLESLKEWWHEEDKNKPPRLLSLMFSKSSQNHGR"
BASE COUNT          541 a          701 c          579 g          519 t
ORIGIN      
        1 gaccgtcggc tccatcgccg tgagagtcaa caattgcaac aggtgattgg caaccatgtc
       61 gcgcaatgct ccggcctctt cgtaatagcc ggcccgtgaa cctacaccca actcttcagc
      121 ggccgtgatc tcaacgtggt caacaaagtt gcgattccag atgggctcaa acatcgaatt
      181 gccgaaacgg aagacgagga tgttctggac ggtgccttta ccgaggtagt ggtcgatgcg
      241 gtaaacctga tcttcagtga agaccttcgc gatctgatcg ttgagcgtgc gagccgactc
      301 cagatcccgg ccaaacggtt tttcaaaaac aatgcgagtc cagccctttt cctgatcggc
      361 gagacccgct tcacccagcc cttccacgac cgtcggtgct aacgagggag gcgtagccag
      421 gtaataaagc cgattccagc cggcaccgtg cgatgcgagt tcttcactga gggccgctat
      481 cttgtgaaag acttcgttgc ccgcgaaatc accggcaatg tagtggagcc gttgcgcaaa
      541 ttcgttccat tggtcatcgc caacctccag cttgccgagt tcccttgccc cttcgcgcat
      601 cattcggcga aattcctcat gggtcatttc attgcgacca acaccgagaa tttgaaattc
      661 cctcatgcag ccctcgcaag cgagatgaaa taacgcaggc atcagtttgc ggcgggttag
      721 atctccggaa gcaccaaaga caacaagcac acacgattcc acctggggca aagggggatg
      781 ctccagctcc actgctcttg cttctacgat gacgtctgcc attagctact tcttttctgc
      841 atgcccgcca aactggaagc gcattgcaga caatactttg ttcgcaaaat cagcttcgcc
      901 acgagaagta aagcgctcaa acaaagccgc actcagcact ggcacgggca cactctcttc
      961 aactgcggca ttgatggtcc agcgaccctc acctgagtca gaaacgcgac cggaaaactc
     1021 ttccaaatgt ggttgttcga gcaaggcgat ggccgtcagg tccagcagcc aggaagcaac
     1081 cacactgcct cgccgccaga gctcggtaat ctcggcaagg ttgaagtcat actgaaagtg
     1141 ttcaggattg cggagtggcg aggtttcagc atcagtcgcc cggtcctgtt tgcccgcatt
     1201 cgcgtggaac agaatgttca gcccttcagc ataagcctcc atcaatccat actcgatacc
     1261 gttatggacc atcttgacaa agtggccggc gcccgatgga ccacaatgca gatagccctc
     1321 ttccgccgtg cttgcattgt tgtcacggcc gggggtgcga ggggcagatc cgcggcccgg
     1381 tgcgagagtt ttgaagattg gatccaggtg ccgaacaact tcgccctcac caccaatcat
     1441 ttgacaataa ccgcgctcgc cgccccagat tccgccgctg gtcccaacgt ccacataatg
     1501 aatgcccctg gccttgagtt ccttcgcacg cctgatatcg tcaacgtagt aagagtttcc
     1561 gccatcaatg atgatgtcgc cggattcggc ggcaagcagc agatcctgca acgtcttgtc
     1621 tactaccgcc gcaggtatca tcagccagat tgcgcgcggt ggatccagct ttgagataaa
     1681 ttcgctggga gtcgacgcgc cgagagcgcc ttcccgaaca agcgtctcga ctgccacggc
     1741 gtcacgatcg tagaccgtgc actgatgtcc gccacgcaac aagcgcttca ccatattcgc
     1801 gcccattcgc cctaacccga tcattccgat ttgcatcact ccaactcctg cctgctgttt
     1861 acttgattgt ttgtttcatc cgctaccgtc catggttctg acttgatttg ctgaacatca
     1921 gcgagagcag ccgcggcggc ttgtttttgt cttcttcgtg ccaccactcc ttcagactct
     1981 caagcgacaa cccattcctt gccgtaccgt ccaggaaatc agagacatga tgaacaaacg
     2041 aatcgatttg cgtgactttc tgatcacgcg tgaaattcgc ttttgtacct gcgtactggc
     2101 gatacgggtg caactcgcaa agaaaaagtc ggcccccatc acgcaggcaa cgggaagact
     2161 ctgcgaaaat gaaatcaatc tctttaatgt gttccagcac caggctacaa gttaccagat
     2221 cataagactc gtcatcgcag ggccagcggc tggtgatatc ggcgactgca aaggacacgt
     2281 ttgactgccc ggtttcgccg agcctgacct tagctcgatc gatcatcgcc tgcgaaaaat
//
LOCUS       DASUEX010000133         2176 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_599308, whole genome shotgun sequence.
ACCESSION   DASUEX010000133 DASUEX010000000
VERSION     DASUEX010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_599308"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..862)
                     /gene="thiL"
                     /locus_tag="VFV61_02260"
     CDS             complement(<1..862)
                     /gene="thiL"
                     /locus_tag="VFV61_02260"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEX4899322.1"
                     /translation="MKDEEQRRIGFTIQTRSSSQRNEFDLIQSVRQRAVKQPLLARHH
                     PHHASLLQGIGDDGAVIKQQAGRETVITTDLLVEDIDFRRDSTPVAFLGHKALAVSLS
                     DLAAMGARPRWSLLALGIPQDLWETEFVDQFYQGFFALAERYDVRLIGGDVSRTPEKV
                     VIDSIALGECVAERSVYRFGAKPGDLIYVTGSLGAAAAGLRLIERGARIGQEHGEEPE
                     WKLVQELLKRQLCPEPRVGWGLVLGEERLASGMIDISDGLSSDLNHLCSESNVGALIR
                     ANQLPVDAPVA"
     gene            complement(877..>2176)
                     /locus_tag="VFV61_02265"
     CDS             complement(877..>2176)
                     /locus_tag="VFV61_02265"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEX4899323.1"
                     /translation="GHVALLTTSLADFHEVDALQLKSYNHAASHISSAAKIGGSLQPV
                     TTSPTVYRLASRLDEIGFSDIVNIRNRIMKLRASGATVYQFEGGEPYMNTPDFIKAAM
                     VRAIEENKTRYAPSSGIPELRAAIAAKLREKNQIPAEDKDVIVLNGGMQGLFGAFQSV
                     VNPGEEVLIFSPYWTPIKDLVYHCEGKPVLVPTSEARRDGFEATLERFANDRTRAIYF
                     NTPQNPTGVVFTPDEARAVARFAQARDLIVIADEAYEDLVYDGKHFSIASIAGMFERT
                     ISCFTFSKSYAMTGWRLGYTVATAPWMTGLQKTTLYSSNGVSTPTQWAGLAAFTNHSK
                     LLEEHNRAYRERRDLLLAGLNELGFTCELPAGAFYAFPDVSRISRDSREAAEILLDRA
                     QVATVPGVVFGEHGEGHLRFSFSTSLETIEAGLESLRRNL"
BASE COUNT          508 a          573 c          596 g          499 t
ORIGIN      
        1 ctgcaactgg tgcgtcaacc ggaagttggt tagctcgtat gagcgcgccc acattgcttt
       61 cactacagag gtgatttagg tctgaggaca agccatcgct gatatcgatc attccagaag
      121 caagccgttc ttcgcctaat accagtcccc acccaactcg tggctcgggg caaagttgcc
      181 gctttaataa ttcctgaaca agtttccatt cgggctcttc accgtgctct tgaccgattc
      241 gcgccccccg ttcgattaaa cgcaaccccg cggcagcagc gcccagcgac ccggttacgt
      301 aaatcaaatc acctggtttc gctccaaaac gatagactga acgttcagca acacactctc
      361 ccagcgcaat ggaatcgatc acgaccttct caggagttcg cgagacatcg ccgccgatta
      421 gtcgcacgtc gtaacgttcg gccagagcga agaagccctg ataaaactga tcgacaaatt
      481 cggtttccca aagatcctgc ggtattccca gggctaagag cgaccagcgc ggccgcgcgc
      541 ccatcgcagc gaggtcggac agggacaccg caagggcttt gtgaccgagg aaggccactg
      601 gtgtgctatc acgtcgaaaa tcaatgtctt cgaccagcag atcggtggta atgacagttt
      661 cgcgcccggc ctgctgtttg atcacagcgc cgtcatcgcc aataccttga agcagggaag
      721 cgtggtgcgg gtgatgtctg gcgagcaggg gttgcttaac agctcgctgg cgaactgact
      781 ggatcaaatc aaattcgttg cgttgagaac tggatcgggt ttgaatagtg aagccaatcc
      841 gacgctgctc ttcatccttc attcctcgtc cttcgtttag agattgcgtc gcagcgattc
      901 gagtccggct tcaattgttt ccagtgaggt ggaaaaggaa aaacgcagat gaccttcgcc
      961 atgttcacca aacacgaccc ccgggactgt tgctacctgg gcccgatcca gaagtatctc
     1021 tgccgcttcc cggctgtcac gactaattcg cgagacgtca ggaaatgcgt aaaaagcccc
     1081 ggctggtaat tcgcaagtga agccaagttc attcagacct gcgagtagta gatcgcgtcg
     1141 ctcacgatat gcgcgattgt gttcctcaag aagctttgaa tggttggtga aggccgcaag
     1201 cccggcccac tgtgtaggcg tggaaactcc gttggaagaa tagagagtcg tcttttgcag
     1261 tcccgtcatc cagggtgcgg tggcaactgt gtagccgagg cgccaaccgg tcatcgcgta
     1321 ggacttcgaa aaagtaaagc acgatatagt tcgttcaaac atgccggcga ttgaagcgat
     1381 ggaaaagtgc ttgccatcgt agaccagatc ttcgtatgcc tcatctgcga tcacaatcag
     1441 atcgcgtgcc tgagcaaagc gagcaacagc gcgagcttca tctggcgtga agacaactcc
     1501 ggtcgggttt tgcggcgtgt tgaagtagat ggcgcgcgtg cgatcgttgg caaagcgttc
     1561 gagcgttgct tcaaaaccgt cgcgccgcgc ttcacttgtg ggcacaagga cgggtttgcc
     1621 ttcgcagtga taaacgagat ctttgattgg cgtccagtag ggcgagaaga tcaaaacttc
     1681 ttcgccagga tttactacgg attgaaacgc gccaaagagt ccctgcatgc cgccattcaa
     1741 aacgatgacg tctttatcct cagcgggaat ctgattcttc tcgcgcagct tggcggcaat
     1801 cgcggcacgt aattccggaa tgcccgatga cggtgcgtag cgagttttgt tttcttcaat
     1861 cgcacgtacc atcgctgcct taatgaagtc gggcgtgttc atgtaaggct caccgccttc
     1921 aaactgatag acggtggcgc cggaggcgcg cagcttcatt atgcgatttc gaatgtttac
     1981 gatgtcggaa aagccaatct catcaagccg cgaggcgagg cgataaacgg ttggtgacgt
     2041 ggtcactggc tgcaatgaac ctccgatttt tgcggctgag ctgatgtggg atgcagcgtg
     2101 attataactt ttgagttgaa gtgcgtctac ttcatgaaag tcggctaatg aagtggttaa
     2161 aagggcaaca tgtccc
//
LOCUS       DASUEX010000134         2414 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_942963, whole genome shotgun sequence.
ACCESSION   DASUEX010000134 DASUEX010000000
VERSION     DASUEX010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_942963"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="VFV61_02270"
     CDS             <1..487
                     /locus_tag="VFV61_02270"
                     /inference="COORDINATES: protein motif:HMM:NF012447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HEX4899324.1"
                     /translation="AIALTDLMSITERRIDRLVNPDLNENLPPFLTLEPGISSGFMMM
                     QVTAAALLNEAKVFAHPASVDNVPTDGGKEDHVSMGMTAATKLRTIVNNAEAVTAIEL
                     ITAAQGLEYRAPLLPGRGVKAAYDVVRRFVPRLGQDRSMAPDIERLTAAIVAGEFDEL
                     I"
     gene            550..858
                     /locus_tag="VFV61_02275"
     CDS             550..858
                     /locus_tag="VFV61_02275"
                     /inference="COORDINATES: protein motif:HMM:NF024845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HEX4899325.1"
                     /translation="MTDIFDERRKGLEEEYFHKKNQEAIERLRAKMNVAAEAKAAGAS
                     TMQCPRCDGGTLEESKFEEVVIDRCNKCGGVWLDFGELEQLTKTEKAGWLGRLWSSSE
                     "
     gene            867..>2414
                     /gene="hutU"
                     /locus_tag="VFV61_02280"
     CDS             867..>2414
                     /gene="hutU"
                     /locus_tag="VFV61_02280"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: protein motif:HMM:TIGR01228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HEX4899326.1"
                     /translation="MSQQTVRAPRGATLTCKSWHQEAALRCLMNNLDPDVAERPSELI
                     VYGGAGKAARNWPAFAAIVAALKELENDETLLVQSGKPVGIFRTHEYAPRVLIANSNL
                     VGAWANWEHFHELERKGLMMYGQMTAGSWIYIGTQGIVQGTFETFAAMADKHFGGDLS
                     GKLLVSGGMGGMGGAQPLAATLNGAVFLGIDVDPARIERRVRTGYCDRMALSIDEALE
                     ICEAAREEKRAVSVGLVGNCAEVLPEIVKRGVKVDAITDQTSAHDPLNGYIPAGVTLS
                     EAADLRKANPAEYWTRSLESMAQHVEAMLALKRAGAVAFDYGNNIRKFAFDAGCKDAF
                     EIPGFVPEYIRPLFCEGKGPFRWVALSGDESDIRKTDDLALELFPDDKTLNRWLRLAR
                     DRVQFQGLPARICWLGYGERAEMGEAINDLVKRGDLQAPVAIGRDHLDTGSVASPFRE
                     TEAMKDGSDAVADWPILNALLNTASGASWVSFHHGGGVGIGYSLHAGQVSVADGSPEA
                     AKRLNRVL"
BASE COUNT          547 a          613 c          743 g          511 t
ORIGIN      
        1 agcaattgcg ctcacggatc tgatgagcat caccgaaaga cgtatcgatc gtctggtcaa
       61 ccctgatctg aatgagaatc tcccaccgtt cttgacactg gagcccggta tctcttcggg
      121 tttcatgatg atgcaagtca ctgcagccgc tttgctgaac gaggctaagg tgtttgccca
      181 tcctgcgagc gttgacaatg ttcccaccga cggaggcaag gaagatcacg tatccatggg
      241 catgacggcc gcgacaaaat tgcgaactat agtcaacaat gcagaagccg taactgcgat
      301 tgaactgata acggcagcgc aaggtttgga gtatcgcgcg ccattactgc caggcagggg
      361 agtcaaggct gcatacgatg tcgttcgaag gtttgtcccc cgactcgggc aggatcgttc
      421 aatggcgcct gatatcgagc gactgactgc ggcaatcgtc gcaggtgaat ttgacgagtt
      481 gatctagatc acccaccggt acagggtctg aagcaagaca aattatagga agaggaaaag
      541 agggagataa tgaccgacat attcgatgaa cgccggaagg gcctggaaga agagtatttt
      601 cataagaaga accaggaagc catcgaaaga ctgcgggcga agatgaacgt ggctgctgaa
      661 gcgaaagccg ccggcgcctc caccatgcaa tgcccgcgct gcgatggtgg aactcttgaa
      721 gagtcgaagt ttgaggaagt cgtaattgat cgctgcaaca agtgtggcgg tgtctggctt
      781 gatttcggcg agttggaaca gctcaccaag actgaaaaag caggttggct cggccgattg
      841 tggagtagtt cggaatagag aggtctatgt cgcagcaaac agttcgtgcg ccccgagggg
      901 caacgctcac ttgtaagagt tggcaccagg aggccgccct gcgttgcctc atgaacaacc
      961 tggatccgga tgttgcagag cgtccaagtg agctgattgt ctacggcggc gccggaaaag
     1021 cggcccggaa ttggccggcc tttgcagcta tcgtcgccgc cctgaaggag cttgagaacg
     1081 acgagacact gttggtccaa tccggcaaac cagtgggtat ctttcgcacc catgaatatg
     1141 caccacgcgt tctaatcgca aattcaaatc tggtgggcgc ttgggcgaac tgggaacact
     1201 tccatgagct cgagcgcaag ggcctgatga tgtacggcca gatgacagcc ggtagttgga
     1261 tttacatcgg cacccaggga atcgtgcaag ggactttcga gacctttgcc gctatggccg
     1321 ataagcactt tggcggcgat ctcagtggca agctgctggt ttccggggga atgggcggca
     1381 tgggcggagc gcagccactg gctgccacct tgaatggcgc cgtctttctc gggatcgacg
     1441 tggatcccgc tcggatcgaa cggcgagtaa gaactggcta ctgcgatcgc atggcgctct
     1501 ccatcgatga agccctggag atttgtgaag ccgcccgcga agagaagcgt gcagtttcag
     1561 tcggcctggt aggcaattgt gctgaagtgc tgccggagat tgtgaagcga ggcgttaagg
     1621 ttgacgctat caccgatcag acaagtgctc acgatcctct caatggttac attcctgctg
     1681 gtgtgacact cagcgaagca gcggatttgc ggaaggccaa tcccgcggag tattggacca
     1741 ggtcgctcga gtcgatggcc caacatgtgg aagcgatgct ggcgctcaaa cgagcaggtg
     1801 cggttgcgtt tgattatggc aacaacatcc ggaagtttgc cttcgatgcc ggctgcaagg
     1861 atgctttcga gattcccggt ttcgttcctg aatacatcag gccactcttt tgtgaaggaa
     1921 agggaccatt tcgttgggtt gctctgtcgg gcgacgaatc tgacatccgc aaaacagacg
     1981 acctcgcact cgaactgttt ccggatgata agactttgaa tcgctggctt cgtctggcgc
     2041 gcgatcgggt gcagtttcag ggtttgccgg cccgcatctg ctggctcggt tacggcgaac
     2101 gcgccgagat gggcgaggcc atcaacgatc ttgtcaagcg cggcgatctg caggcgccgg
     2161 tagcaatcgg ccgcgatcac ctcgacaccg gctcggtcgc atctccgttt cgtgaaacag
     2221 aagcgatgaa ggatggatcg gacgcggtag cagactggcc aatcctgaat gccctgctca
     2281 acaccgcgag tggcgcgagt tgggtgtctt tccatcacgg cggcggcgtt ggtatcggct
     2341 actcgctgca tgccggtcaa gtgtcggttg ccgatggctc gccggaagca gctaaacgtc
     2401 tgaatcgtgt gctg
//
LOCUS       DASUEX010000135         4389 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1057561, whole genome shotgun sequence.
ACCESSION   DASUEX010000135 DASUEX010000000
VERSION     DASUEX010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4389
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1057561"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..854)
                     /gene="kdsA"
                     /locus_tag="VFV61_02285"
     CDS             complement(6..854)
                     /gene="kdsA"
                     /locus_tag="VFV61_02285"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF003543.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HEX4899327.1"
                     /translation="MQTKSFQVGNATFGDGRLTIIAGPCVIESSEHALMMARECAQRA
                     REAGLDFVYKSSFDKANRSSVQSFRGLGMEAGLEVLRRVKEEVGVPVLTDVHDVSQIR
                     PVAEVVDVLQIPAFLSRQTDLILAAAQSGRAVNVKKGQFLAPADARNIVDKARAAGCE
                     KILLTERGVSFGYNNLVVDMRSFPIMSEFGVPVVFDVTHSLQLPGGLGHATGGLSQYI
                     EPLARAGVAAGVDAVFMEVHDHPERALSDGPNALPLERMQSLLNILRDIHELVGTISA
                     RETDNR"
     gene            complement(895..2418)
                     /locus_tag="VFV61_02290"
     CDS             complement(895..2418)
                     /locus_tag="VFV61_02290"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX4899328.1"
                     /translation="MLHCPICGRLYPTDVKVCPEDNAALQADATIATDGPVDPLIGHT
                     LDDKYRIEARLGIGGMGTVYRARHLLIDRAVAVKVLSQRFVEDEAAQMRFRREAKAAG
                     RLQHQNAVAVTDFGQTTDGYVYIVMELLEGRTLRDVLAKEAPLDPARAVALMLQTSAA
                     VAAAHEAGIIHRDLKPANIFIVHNSEVPAVVKVLDFGIAKLAAETLEEDEAHTLTQVG
                     AMIGTPRYMSPEQCDGAELSPAADVYSLGVILYEMLTGMVPFSGSTPLAIAMKHTAEA
                     PRPPREFVISIPAELEAVVLHALEKAPENRPADAAEFRRELLATAERLGLEHGSTTSA
                     PDLQSLRDAGTQSPSGRLVVDLARLRENRAASSSANEMTVIRQSRDPEVVKEASGAGN
                     IHTGYSFPRVSVQLGQTFERARQKPIALLGLLVVLAAIGGVGYFTTRAWRTATPAAEQ
                     AIITPTPTPTPDATPTPTPAPSPKREQAKPPVNANKKKESKVGSALRKVGRILKKPF"
     gene            complement(2631..4328)
                     /locus_tag="VFV61_02295"
     CDS             complement(2631..4328)
                     /locus_tag="VFV61_02295"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003792.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEX4899329.1"
                     /translation="MPKYIFVTGGVVSSLGKGLAASSIGCLLEARGLRVALIKLDPYI
                     NVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFTHAHLSQANNWTTGRIYLSVIEKE
                     RRGDYLGKTIQVIPHITNEIKDAVRAVAEKDVLDVVIVEIGGTVGDIESLPFLEAIRQ
                     MGNEEGAGNALFVHVTLVPFIAAAGELKTKPTQHSVRDLREIGIAPDVLLCRSEKPLS
                     HDLRSKIALFCNVKDSAVISAQDVDTIYDVPLAFHEQGLDELIITQLGFGARAAQIDL
                     AQWRELVGTIREPSGGETSIAIVGKYVELEDSYKSLREALTHGGVANKLRVSVKWIES
                     EELMDDDYESRLRDFDAILIPGGFGKRGVAGMIRAISYARKSRTPFFGICLGMQTSCI
                     EYARNVCGLKDADSTEFDLETPYPIIFKLRDLVDVDELGGTMRLGEWPCELKPESLAS
                     EIYGGAAEISERHRHRYEFNPDFRETLERAGLTFSGVSPDQKFIEIVELPRDVHPWFL
                     GCQFHPEYKSKPLDAHPLFSSFVRAAYENRLQNETSMDKNGEAELVVHERVTVTSAD"
BASE COUNT          962 a         1289 c         1187 g          951 t
ORIGIN      
        1 agacattacc tgttatctgt ttctcgagcc gagattgttc caactaactc atgaatgtcg
       61 cgcaaaatat ttaagagcga ctgcatgcgt tcgagcggca aggcgttcgg gccatcggag
      121 agcgcccgtt ccggatgatc gtggacttcc ataaaaaccg catcaacacc ggccgcaacc
      181 ccagcgcgcg cgagcggttc gatgtactgc gacaagccgc cggttgcatg acccagcccg
      241 cccggcagtt gcagcgaatg cgtcacgtcg aaaacaacgg gaacgccgaa ctcgctcatg
      301 atcggaaaag atcgcatgtc aacgaccaga ttgttatagc cgaagctcac gccgcgttcg
      361 gtcagcaaga tcttctcgca gcctgctgcc cgtgccttgt cgacgatgtt gcgagcatcg
      421 gccggcgcga ggaattgccc cttcttaaca ttcacggccc gccctgattg cgccgcggct
      481 agaatcaaat cagtttgccg cgacaagaac gcgggaattt gcaggacgtc aacgacctcg
      541 gcgaccggtc gaatttgcga aacgtcgtgc acatcggtta gcaccggaac tccgacttct
      601 tcttttaccc ggcgcagaac ctcaaggcct gcttccattc caaggccgcg aaaactctgt
      661 accgaggagc gattggcctt gtcgaaagac gacttgtaaa cgaagtcaag tccggcctct
      721 cgcgcgcgtt gcgcacactc ccttgccatc atcagggcgt gctcggatga ttcaatcacg
      781 caaggccccg cgatgatcgt caagcgccca tcaccaaagg tggcattgcc cacctgaaac
      841 gatttcgttt gcacgtttgg atttaaccct aactgaattg ttcgagcaac tagcctagaa
      901 cggtttcttc aatatgcgac cgaccttcct taacgcggaa ccaaccttcg attctttttt
      961 cttgttcgca ttcacgggcg gctttgcttg ttcccgtttc gggctgggcg cgggtgttgg
     1021 tgtcggcgtc gcatcgggcg ttggagttgg cgtcggagta atgattgcct gctccgctgc
     1081 cggcgtcgca gtccgccaag ctcgcgttgt gaaataaccc acgccgccga ttgccgcaag
     1141 taccacaagc aatcccagca aagcgatcgg tttttggcgg gccctttcga aagtctgacc
     1201 tagctgcaca ctaactcgcg gaaaagagta acccgtatga atgttccccg cgccgctggc
     1261 ttcctttact acctcggggt cacgcgactg ccggatgact gtcatttcgt tggcactgga
     1321 acttgcagca cgattttcac gcagtcgcgc cagatcaacg accagtcgtc ctgagggcga
     1381 ttgcgttccc gcatcgcgga gtgattgcaa atccggagcg ctggtagtcg acccgtgctc
     1441 caggccgagc cgttcggcgg tggccaacaa ctcgcgacga aattcagcag catctgcagg
     1501 acgattttcg ggcgctttct cgagggcgtg cagcactact gcctccagtt cagctggaat
     1561 cgaaattaca aactcgcgcg gcggccgcgg cgcctcggca gtgtgcttca tggcaatggc
     1621 taacggcgtc gatccagaga acggcaccat accagtcagc atttcataaa gaatcacacc
     1681 cagactgtaa acgtcggccg ccggactgag ctcggctccg tcacattgtt ccggagacat
     1741 gtagcggggc gtgccgatca tcgcgccgac ttgcgtcaac gtgtgcgctt cgtcttcctc
     1801 gagagtctca gcggcgagtt tggcaatgcc aaagtcgagc accttcacca ccgccggcac
     1861 ttccgaatta tggacgatga aaatgtttgc tggtttcaga tcgcgatgga ttattccggc
     1921 ttcatgcgcg gcagctacgg cagcggaggt ttgcagcatg agggcaaccg cgcgggcggg
     1981 atcgagcgga gcttccttcg ccagcacatc gcggagcgtg cggccttcca gcaactccat
     2041 cacgatataa acatagccat cggttgtttg cccgaagtcg gttacggcga cggcgttttg
     2101 atgttgcagg cggccagcag cttttgcctc acgacggaag cgcatctgcg ccgcctcgtc
     2161 ctccacaaac cgttgactca gaactttgac cgccaccgcc cgatcaatca gcagatgtct
     2221 ggcgcggtac accgtgccca tgccgccgat tccgagtcgt gcttcgattc gatatttgtc
     2281 atcaagggtg tgcccaatga ggggatcgac aggaccgtca gtcgcaatcg tggcatcagc
     2341 ctgaagtgcc gcgttgtctt ccggacagac ctttacatcc gtcgggtata gccggccgca
     2401 tattgggcaa tggagcatat gatctcgtgg ggaacgttga gctcaatgat tatcccgcaa
     2461 aacgttgtga gatgcaccta agcgacggac ccgtggggga cgcaatcaaa acgtacgaag
     2521 tctttcaggt aggtagtagt gagtcttgat gtgggtggcg cggccggcgc cgcacatccg
     2581 gcggcaaagc cgaaagctcc cactactcta agtcttgagg caaggctggc tcaatcggca
     2641 gacgtcacgg tgacacgctc gtgcaccacg agttccgctt cgccgttctt atccatcgac
     2701 gtttcgttct gcaaacgatt ctcatacgcc gcgcgcacga aagaagaaaa cagcggatgg
     2761 gcatcgaggg gtttggattt gtattcagga tgaaactggc aaccgagaaa ccaggggtgc
     2821 acgtcgcgcg gtaactcaac aatctcgata aacttttgat cgggcgatac gccactaaag
     2881 gttaggccgg ctcgctcgag cgtctcgcgg aagtccgggt tgaattcgta gcggtgacga
     2941 tgccgctcgc tgatctcggc ggcgcctccg tagatttcgc tcgcgagcga ttctggtttc
     3001 agttcgcaag gccattcacc tagtcgcata gttccaccca gctcatccac atcgaccaga
     3061 tctcgtaact tgaaaatgat tggataaggc gtctccaaat cgaattcggt ggagtcggca
     3121 tctttaagcc cgcaaacgtt tcgcgcatac tcaatgcaag acgtctgcat tcccagacag
     3181 ataccaaaaa acggtgtgcg actcttacgc gcatatgaaa ttgcgcggat cattcccgcc
     3241 acgccccgct tgccaaagcc gcccggaatc aaaatcgcgt caaaatcacg cagacgagac
     3301 tcgtagtcat cgtccatcaa ctcttccgac tcaatccatt taacgctgac gcgcagttta
     3361 ttggcgacgc caccgtgcgt tagcgcctcg cgcagagact tgtacgagtc ttccaactcc
     3421 acatacttgc ccacgatggc gatcgaagtt tccccgcctg agggctcgcg gatagttccc
     3481 accagttcgc gccactgtgc cagatcaatc tgtgccgccc gcgcgccaaa accaagctga
     3541 gtaatgatta actcgtccag cccctgttcg tggaacgcca gtggaacgtc atagatcgta
     3601 tcgacatcct gagcgctgat cactgctgaa tctttaacgt tgcagaagag ggcaatcttt
     3661 gagcgcagat catgcgagag aggtttttct gaacggcaaa gcaggacatc gggagcgata
     3721 ccgatctcgc gtagatcgcg aaccgaatgt tgagtcggct tggtctttag ctcgccggcg
     3781 gcggcgatga aaggcaccag agtcacgtgc acaaaaagcg cattgccggc gccttcttcg
     3841 ttgcccattt gccggatagc ttccagaaat ggtagcgact caatatcgcc gactgtcccg
     3901 ccgatctcga caatcactac gtcgaggaca tctttttccg cgacggcgcg cacggcatct
     3961 ttaatctcgt tggtgatgtg gggaattacc tgaatcgtct tgcccagata gtcgccgcgg
     4021 cgttccttct cgatcacgga caagtagata cggccggtag tccaattatt cgcctgcgaa
     4081 agatgcgcgt gagtgaagcg ctcgtaatgg cccaggtcga gatcggtttc agcgccgtca
     4141 tcggtaacga agacctcccc gtgctgaaac ggcgacatgg tgccgggatc gacattgata
     4201 tagggatcaa gcttgatcag ggcaacgcgc agtcccctcg cctcgagcaa gcaaccgatc
     4261 gacgaagccg ccaggccttt acccaggctc gaaaccaccc cacccgttac aaagatgtat
     4321 ttaggcattg cgttctcctc tagcttccac ttttcgatct taataggcca cagatggaca
     4381 cggattcac
//
LOCUS       DASUEX010000136         3280 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_828455, whole genome shotgun sequence.
ACCESSION   DASUEX010000136 DASUEX010000000
VERSION     DASUEX010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3280
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_828455"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(242..688)
                     /locus_tag="VFV61_02300"
     CDS             complement(242..688)
                     /locus_tag="VFV61_02300"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX4899330.1"
                     /translation="MPEVIGIDHIYVTVSDLEASEQFYDRVMIDVLAFRKNKFSVAGD
                     PHIQYFNRYFGFVLRPSRASAKHDPYSPGLHHFCLRVDSAADVHAVANQLRALGVNAT
                     EPRLYPEYASDYVATFFEGPDGIRLEVTNYRQERRERHDHWHRGAG"
     gene            complement(764..1825)
                     /locus_tag="VFV61_02305"
     CDS             complement(764..1825)
                     /locus_tag="VFV61_02305"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium chain dehydrogenase/reductase family
                     protein"
                     /protein_id="HEX4899331.1"
                     /translation="MKHRRIIVPHYGGPDALQVLEEERPEPMHGQVRVRVLSAGVALP
                     DIMAREGIHPETPQVPFTPGWDLIGVVDRLGTGVSGIAPGQIVAAMPISGAYAEFVCL
                     PEGELVPVPSGLDATEAVSLVLNYITAHQMLHRSARVRPGQRVLIHGAAGGVGTALLQ
                     LGHLAGLEMYGTCSSRGASAVSDLGGIPIDYQRQDVVEEIHRLTREGVDAVFDPIGGT
                     HLWHSRKALRPGGSVVGYGLITSIRGEGLTSGRPGRRQRFRGTAIFGLYIVGGWLLPG
                     RKRVVPYSIQTLKRLKPEWFRQDLIALFDLLQQKKIKPLIAQRLPLTEARHAHELLGK
                     GGVIGKIVLNVSSLESGAG"
     gene            complement(1822..1962)
                     /locus_tag="VFV61_02310"
     CDS             complement(1822..1962)
                     /locus_tag="VFV61_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899332.1"
                     /translation="MNIAPNNAINMDSEKRRAFIAPLFTAGYGKRYAAQARATGNSGN
                     DV"
     gene            complement(1963..2421)
                     /locus_tag="VFV61_02315"
     CDS             complement(1963..2421)
                     /locus_tag="VFV61_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899333.1"
                     /translation="MTNNSAVKILVAGLVGGFIGNGVLGAIFSSSPIQSILYNPEWQS
                     QLFIEITSKRNVPVSVAGLVILSIVHSWLFSVLMPSIPGKTWLRKGLFWGLTIWLMYW
                     LFQEWFIYNTLLGEPLILNALELTILILGALVEGIIIAFFLARKPSGGTV"
     gene            complement(2418..2774)
                     /locus_tag="VFV61_02320"
     CDS             complement(2418..2774)
                     /locus_tag="VFV61_02320"
                     /inference="COORDINATES: protein motif:HMM:NF016854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HEX4899334.1"
                     /translation="MPKPNVKSGHKAILDSLLLNMPSVVDGKMFGYPAYFVNGKLFAC
                     VYGEGVGVKVPEEVANKLLAEKHVVPFQPLGKPKMREWIQINRARPTDYGKDINVFRT
                     SIEFVSQLQTTKRKKK"
     gene            complement(2923..>3280)
                     /locus_tag="VFV61_02325"
     CDS             complement(2923..>3280)
                     /locus_tag="VFV61_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899335.1"
                     /translation="MRWTAFPREIDREKLDRVILRKIGGVTYDLRIWDEDADQRLDRP
                     WNLQWRNRLVYERTGLAAPHHTLEVPLAPASRYYWSARARFVVDGRTMVTRWMRMSKC
                     FSNDLFLGYPYFNTSK"
BASE COUNT          703 a          967 c          921 g          689 t
ORIGIN      
        1 tggttcgttg cacggcagtg cccgacacgc gtcgctgcga gctcattcca acctcccctt
       61 catgtttccc gtcgacatcc gggccgcttc gggtcgaaag cagacatgga cgtcaggcga
      121 tttctcactt cgaacctcgg cattacgcga ccggcgtgcc acctaacgtt ccggttcagc
      181 ggcggcgcgc cgatctacgt gccttggcac tttatccatc accgtccgct gcaaccggtt
      241 gttagccggc gccgcgatgc cagtgatcat ggcgttccct ccgctcttgt cggtagttgg
      301 taacctctag acgtatacca tccgggcctt caaagaaggt tgctacgtag tcgcttgcgt
      361 actctggata caaccttggt tctgtagcgt tgactccgag ggcgcgaagt tgattcgcaa
      421 ccgcatgaac atcagccgcg gaatcgacac gcaggcagaa gtgatgcaga cccggagagt
      481 aagggtcgtg tttcgcggac gcgcgggacg gtctgagcac aaagccgaag tatcgattga
      541 agtactgaat gtgcgggtcg cccgcgacac tgaatttgtt cttgcggaag gcgaggacgt
      601 cgatcatcac ccggtcgtag aactgttctg atgcctcaag atcagacacc gttacgtaga
      661 tgtgatcgat tccgatgacc tcaggcacga gcgagctcca ttggctgtcc ggctaacgct
      721 cggcatcagc ggcgtcgcga acgccgtccg ctgcatgccg ttgttatccc gcgcctgatt
      781 caagcgagga cacgttgagc acgatcttgc ctatcactcc tccctttccg agcaactcgt
      841 gcgcgtgcct agcttcagtg aggggaagtc gctgcgcgat gagcggcttg atcttcttct
      901 gttggaggag gtcaaacagg gcgatcaagt cctgtcgaaa ccattccggt ttgagccgtt
      961 tgagcgtctg aatactgtag gggaccacgc gcttgcggcc cgggagaagc cagccgccga
     1021 cgatgtagag cccgaagatg gcggttccac gaaaacgttg acggcgccct ggacgacctg
     1081 aagtcaatcc ctccccacgt atcgaagtaa tgaggccata gcccaccacg ctcccgccag
     1141 gacggagagc cttgcgggaa tgccagagat gggtgccacc gatggggtca aagacggcgt
     1201 ccacgccctc gcgggtaaga cggtggatct cttccacgac gtcctgacgc tggtaatcaa
     1261 tcgggatacc gcccaggtcg gaaacagccg aggccccccg cgatgaacat gtaccgtaca
     1321 tctccagccc ggcgaggtgc ccaagctgca agagggccgt gccgaccccg cccgccgcgc
     1381 cgtgtatcaa cacgcgctgg cccggtctga ccctggcgga acgatgcagc atctggtgcg
     1441 cggtgatgta gttcagtacg aggctgacag cctcggtggc gtccaacccg gatggcaccg
     1501 ggaccagttc accttccggc aggcagacga actccgcata cgcgccgctg atcggcatcg
     1561 cggcaacgat ttgacccggt gcgattccgg agacaccggt gccgagccga tccaccacgc
     1621 cgatcagatc ccagcccggc gtaaacggca cctgaggcgt ttcgggatga atgccctcgc
     1681 gcgccatgat gtcgggcaag gcgacacccg cggagagcac tctcacccgc acttgaccat
     1741 gcatcggctc ggggcgctct tcttcgagca cctgaagcgc atcaggaccg ccgtagtgag
     1801 ggacgatgat acgcctgtgt ttcatacatc gtttccactg ttgccggtcg ctctggcttg
     1861 tgcggcataa cgtttgccat aaccggcagt gaaaagcggg gcgatgaagg cgcgccgctt
     1921 ctcactgtcc atgtttatgg cgttgttagg tgcgatgttc acttacaccg tgccacccga
     1981 aggtttgcgc gccagaaaaa acgcaatgat tattccttca actaaggcac ctaatatcaa
     2041 gatggtgagc tcaagagcat tcagaatgag tggctcgcca agcaacgtgt tgtagataaa
     2101 ccattcctga aacaaccaat acatcaacca gatcgttaac ccccagaaca gtccttttct
     2161 taaccatgtt ttcccgggta ttgagggcat gagaacactg aaaagccaag agtggacaat
     2221 gctcaatatc acaaggccag cgaccgagac cggtacgttt cgtttcgagg tgatctcgat
     2281 aaagagctgg ctttgccatt cagggttgta aagtatcgac tggatcggag agctggaaaa
     2341 tatggcaccc agcaccccgt taccaataaa gccgccaacg agaccggcga ctagaatttt
     2401 cacggcactg ttgtttgtca ttttttcttc ctcttcgtgg tttgtaattg gctcacaaac
     2461 tctatagagg tacgaaagac gttgatatcc tttccataat ccgttggcct cgcacgattg
     2521 atttgaatcc actcgcgcat tttcggtttg ccaaggggct ggaaggggac aacatgtttc
     2581 tcggctagaa gcttgttcgc gacctcctca ggaaccttga ctcccacccc ttcgccgtag
     2641 acacacgcaa acagcttccc gtttacgaag taggcgggat agccaaacat ctttccatct
     2701 accacgcttg gcatgttgag cagcaaagaa tcgaggatcg ccttgtggcc ggatttgaca
     2761 tttggtttcg gcatatgtac gcctccattc gcacctaacg ttggagttca cccgcgcgcg
     2821 gaggcgggcg aagccggccg tagcacgtcg ggtgcagtga aaagttaggc cccaagcggt
     2881 atgtctccga tcagacttcc ccgaaggcga ccactggcaa tcctactttg acgtgttgaa
     2941 gtatgggtaa cctagaaaca gatcgttcga aaaacactta ctcatgcgca tccagcgcgt
     3001 caccatcgtc cgaccgtcga ccacgaaccg cgcgcgcgcc gaccaatagt agcggctggc
     3061 gggagccagc gggacttcca gcgtgtgatg cggggccgca aggcccgtgc gctcgtaaac
     3121 cagccggttt cgccattgca agttccaggg gcgatcgagc cgctgatcgg cgtcctcgtc
     3181 ccagattctt agatcgtaag tgacgccgcc tattttccgc aggatcacgc gatcgagttt
     3241 ctcgcggtcg atttcgcggg gaaacgcagt ccagcgcatc
//
LOCUS       DASUEX010000137         3552 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1515469, whole genome shotgun sequence.
ACCESSION   DASUEX010000137 DASUEX010000000
VERSION     DASUEX010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3552
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1515469"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(168..710)
                     /gene="mreD"
                     /locus_tag="VFV61_02330"
     CDS             complement(168..710)
                     /gene="mreD"
                     /locus_tag="VFV61_02330"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEX4899336.1"
                     /translation="MPQFKIAAVLALAIILQLTLGEAWPPLSHIDFPLIVVVYVALQR
                     DAWTALIVGTIAGLAIDAPSGGLIGAGGFSKTLTAYLVYLGATRVNLENALIRIPVLA
                     AATLVDGAVYFFWQRSLGHPPSAPFVQTISYNLIGTTVIGTFVLYMLNSVFNPTGTQS
                     RQFASRRRGARRSASFSKRR"
     gene            complement(739..1653)
                     /gene="mreC"
                     /locus_tag="VFV61_02335"
     CDS             complement(739..1653)
                     /gene="mreC"
                     /locus_tag="VFV61_02335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEX4899337.1"
                     /translation="MAATRTQKEIRQRAPIWLVMLLFANFVVMAVDANQRFAQQQTFK
                     GLVQAVFSPIQNATSWVSSTGGGFINEILNFRSTARENQSLKERLAQAELELRNTQEA
                     KTENYRLQGLLALKAEPGAEKVFARVIARDASVWFNTVMINRGSSSGIALNMPVITPG
                     GIVGRVVAVSPVTAQVMLITDEKAAAGAIVGQLGGSGALGSVRGLGNSGLVEMRYVSG
                     LQKVDVGDYILTTGQDGIYPAGLNVGEVIEVKNGTATQPHQIYIRPSARLDQLTEVAV
                     LLYQPPQRPAPEQTLPNVDKTKATPSDR"
     gene            complement(1718..2764)
                     /locus_tag="VFV61_02340"
     CDS             complement(1718..2764)
                     /locus_tag="VFV61_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002621977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEX4899338.1"
                     /translation="MAFKSLFSLFSSDLAIDLGTANTLVYAKGRGIVVSEPSIVAINK
                     VTNQVEAVGRDAKDMLGRTPGNIVAIRPMKDGVIANFEVTEKMLQHFIRKAHNGKTWV
                     SPRVVIGIPSEITQVERRAVEDSAYRAKASEVYLVEEAMAAAIGAGLPITEPHGNMVV
                     DIGGGTTDIAVISLSGIVYSRAVRVAGNEMDEAIISYIKKKYNLLIGERTAEAIKIEL
                     GSAYPLDEPLSYEVRGRNLIEGIPKTITITDEEVREALADSVATIINAVRVALERTPP
                     ELSADIVERGIVMTGGGALLKNLDKRLSIETGLPVSVADDPLASVVLGTGKMLSDFDL
                     LKRVRWENSMTSGM"
     gene            complement(3105..>3552)
                     /locus_tag="VFV61_02345"
     CDS             complement(3105..>3552)
                     /locus_tag="VFV61_02345"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX4899339.1"
                     /translation="VLMEVAAVILLSVRDTDAVARYGGDEFVVVLPDTDTELAGVVAE
                     RIRGKISKYEFTGGKRLQLSLTASFGVATFPYHASSPQQLIACADTAMYEAKAANKNC
                     VRFAAAVNNPPAPLPGTPSTQVETEASPVSAFESQHSEVEDGERPS"
BASE COUNT          790 a          976 c          986 g          800 t
ORIGIN      
        1 gtctcactcc caagagtccc agcaacaaca ccgcgaccat ctgcacagca aagagtctgg
       61 ctcggagatt ttgcgacgtg tcgataaact tcattgcttg tattcagttg tccgtagtca
      121 gttggtcggg gaggtaattc actgtcaacc ttcgcagcga gactcgctca cctgcgcttg
      181 gaaaagctag cactccgtcg cgcaccgcgc cggcgggaag caaactgccg gctttgagtg
      241 cccgtcgggt tgaagaccga gttgagcatg taaagaacga aggtgccgat cacggtcgta
      301 ccaatcagat tataagatat tgtttgcaca aaaggtgcgg aaggtgggtg cccgagggat
      361 ctttgccaaa agaaatatac agccccatcc acgagcgtgg cagctgccag gacgggaatc
      421 cgaatcaatg cattttcgag gttgacgcgc gtcgcaccca ggtagacaag ataggctgtc
      481 aaggttttgg aaaagccgcc cgccccgatc aggccaccgc tgggagcgtc aatcgccaag
      541 ccggcaatcg tgccgacaat caaggccgtc caggcgtcgc gttgcaaagc gacatagacg
      601 acgacgatta gggggaagtc gatgtgcgag agtggcggcc aggcctctcc caaggtgagc
      661 tgcaggatga tggcgagcgc gagcaccgcg gcgatcttga actgaggcat ggtagaactg
      721 tgacaagtgg agcgctgctc acctgtcgct cggcgtcgct ttggttttgt ctacgttggg
      781 cagtgtctgt tcgggtgcag gccgctgcgg tggttgataa agcaacaccg caacctcggt
      841 caattgatcc aaacgcgcgc tggggcggat gtagatctga tgaggctgag ttgcggtgcc
      901 attcttgact tcgataactt cgccgacgtt caagcccgcc ggataaatcc cgtcttgacc
      961 tgttgtcagg atgtaatcgc caacgtctac tttctgcaat ccggagacgt aacgcatttc
     1021 gaccaaaccg gaattcccca ggccccggac cgagcccagt gcgcccgagc cgcccagttg
     1081 tcccacgatt gctccggctg cggctttctc gtctgtgatc agcattacct gagcggtcac
     1141 ggggctgaca gcgactacac ggccgacgat cccaccgggc gtgatcaccg gcatgttaag
     1201 cgcgattcct gacgaactgc cgcggttaat catgacggtg ttgaaccaga cagaagcgtc
     1261 gcgggcgatc acgcgtgcaa aaaccttttc cgctcctggc tccgccttga gcgctaacaa
     1321 gccctgtaag cgatagtttt cggtcttggc ttcctgagtg ttgcgcagct ccagttcagc
     1381 ctgggccaga cgttctttca ggctctggtt ttcccgggca gtactacgaa agttgaggat
     1441 ttcattaatg aagccgccac cggtactcga aacccaggag gtcgcatttt gaatcggaga
     1501 gaatacggct tgcactaacc ccttgaacgt ctgctgttgc gcgaaacgct ggtttgcatc
     1561 cactgccatc accacaaagt tcgcgaacag cagcatcacg agccaaattg gcgctcgctg
     1621 acgtatttcc ttctgtgtgc gagtggcagc catgaggtcg tgaatcgtga tcgtgaatcg
     1681 taaatcgtaa atcgtgaatc gtaagacgtg aatcgcccta catgccgctg gtcatcgagt
     1741 tttcccagcg gactcgtttg agcaagtcaa aatccgaaag cattttgcct gttcccagca
     1801 ccacggacgc caacgggtcg tcggctactg atactggcaa gccggtttca atcgagagac
     1861 gtttatccag gttcttaagg agagctccgc cgcccgtcat aacgattcct cgttcgacta
     1921 tgtcagccga aagctcaggt ggtgtgcgct caagggccac gcgaacggca ttgatgattg
     1981 tagccaccga gtctgccagc gcttcgcgta cttcttcatc ggtgatcgta atagtcttgg
     2041 gaatgccttc gatgaggttt cgtccacgca cctcgtatga gagcggctcg tccagcggat
     2101 aggctgaacc caactcaatc ttgatcgctt cggcagttcg ctcgccaatc aacaggttat
     2161 atttcttctt gatgtaagaa atgatcgctt cgtccatttc attgccggca acgcgcaccg
     2221 cacgcgagta gacgatcccg ctcaagctga tgactgcgat gtcggtggtg ccgccaccga
     2281 tgtcaacaac catgtttcca tgcggttctg taatcggcag gccggcgccg atagcagctg
     2341 ccatcgcttc ttccacgaga taaacttcgg aagctttggc gcgataggct gagtcttcaa
     2401 ctgctcgccg ctccacctgg gtgatctccg aaggaatgcc gattaccacg cgcgggctga
     2461 cccacgtttt gccattgtgc gctttgcgga tgaagtgctg cagcattttc tccgttactt
     2521 caaagttggc aattacgcca tccttcatcg gccggattgc cacgatgttg ccgggcgtgc
     2581 gtcccagcat gtccttggcg tcgcggccga cggcttcgac ctgattcgtg actttgttga
     2641 ttgcaactat cgacggttcg gagactacga tgccgcgccc tttggcgtaa accaacgtgt
     2701 ttgcagttcc cagatctatc gcaagatcac tggaaaatag gctaaaaaga gatttaaagg
     2761 ccatctatta aattcacttc cgtgtggtta agattgagaa atgccgtgga ctacgccgtc
     2821 gggatggtaa ctgactcaaa caaaaaaagt ggctgattcg agagccacgc tatccgggaa
     2881 agacactcca cagactcgcc gacaagtcga agccctttaa gatacaccga atttcaaaga
     2941 ttgtgctatc gggaaattct atttgtcctg aaaaccgatc ttcgcgcacc gctgatcgtt
     3001 taggagggcg ctgacttggc agcgtttcta gtttgcggaa agaaagcggc gactcgctgc
     3061 cgcagtccaa aatggatctt ccagcagttg ttttcaaaag ggaatcagga cggccgttcg
     3121 ccatcttcaa cttcagagtg ttgactctcg aaagccgaga caggcgatgc ttcggtctcc
     3181 acttgcgtgg agggagtgcc gggcaggggt gccggagggt tgttgactgc ggccgcgaaa
     3241 cggacgcagt ttttgtttgc ggccttcgct tcatacatcg ctgtgtcggc gcaggctatg
     3301 agttgttggg gcgaagaagc gtggtaaggg aaagttgcca cgccgaaact agctgtgagc
     3361 gacaattgca gacgcttgcc gccggtaaac tcgtacttac tgatttttcc acgaatgcgc
     3421 tcggcgacca cacccgccag ttcggtgtcc gtatcgggca agacaacaac gaactcatca
     3481 ccgccgtaac gggccacggc atcagtgtcg cgcacggaca aaagaatcac cgccgcgacc
     3541 tccatcagca ca
//
LOCUS       DASUEX010000138         2123 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_828481, whole genome shotgun sequence.
ACCESSION   DASUEX010000138 DASUEX010000000
VERSION     DASUEX010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_828481"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="VFV61_02350"
     CDS             <1..200
                     /locus_tag="VFV61_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899340.1"
                     /translation="VPFRETRLYIQGVVRNTLQYKRLYDEDGNFRPEVGARAIYPSPN
                     SSPSPTDDRIKIRRVIGEEED"
     gene            292..684
                     /locus_tag="VFV61_02355"
     CDS             292..684
                     /locus_tag="VFV61_02355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HEX4899341.1"
                     /translation="MYTLRVRSREREELVEFTDEVQQKLTESAAKEGVVVLYVQHTTA
                     GLTVNENADPDVPRDMLHALRTLIPQHGMGFRHGEENSDAHIKASLVGPSVTVPFSDG
                     KLLLGRWQGIFLCEFDGGRERKVVMMIR"
     gene            734..1873
                     /locus_tag="VFV61_02360"
     CDS             734..1873
                     /locus_tag="VFV61_02360"
                     /inference="COORDINATES: protein motif:HMM:NF037565.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath C-terminal domain-containing
                     protein"
                     /protein_id="HEX4899342.1"
                     /translation="MPEDPKPGIEDFRNGPPGIEPAQTSITAIIGNFNSSAAERPVLL
                     TSEADLNDKLNDNTSPSAQAVAQFFVNGGRECWVVCAKPTEPKLDRVEALKSAIESLD
                     QIDAPNLLSIAAASELDATECAEVIAFAAELCKTRNSFLLVDPPTKLHTPDDILTWLD
                     GNPALRIENAALYFPWVRIADRSNPAQQVRLPPSTTMAGVIARVDLNRGVWKAPAGRE
                     ATLIAVAGLDYSLSDKENDRLNRHGVNCLRTFPDSGPVCWGARTVSLNNEWRYVPVRR
                     LALFIERSIYQSTQWAIFEPNAEALWANVRAVIEDFLVGLWRQGALAGIKPEEAFFVK
                     CDRTTMTQNDLDNGRLIALVGIAPLKPSEFVIIRIELMTAGASEK"
BASE COUNT          505 a          564 c          597 g          457 t
ORIGIN      
        1 ccgtgccgtt tcgcgaaacg cggctctaca ttcagggcgt ggtgcgtaac acgctgcagt
       61 acaagcggct ctatgacgag gacggaaatt ttcgtcccga agtcggcgcg cgtgcaatct
      121 atccttcgcc gaattcgtca ccgtcaccaa ctgacgaccg aataaaaatc cggcgagtga
      181 ttggtgaaga agaagattaa ttatccgccg attacgcaga ttacacagac tagacacacc
      241 atttggcgtg gccgttcctg tgaatctgcg taatcagcgg atcatcacgc catgtacaca
      301 cttagagttc gttcacgtga acgagaagaa ctggttgagt tcactgacga agttcagcag
      361 aagttaactg aatcagccgc gaaggagggc gtggtcgtgc tctacgtcca gcatacgact
      421 gcgggtctaa ccgtcaacga gaacgccgac cctgatgtcc cgcgcgatat gttgcatgcg
      481 ctgcgcacat tgattccgca acatggcatg ggctttcgtc acggtgaaga gaattccgac
      541 gcgcatatca aagcaagttt ggttggtccc agcgtcaccg ttcccttttc cgatggcaaa
      601 cttctcctcg gccgctggca ggggatcttc ctgtgcgagt ttgacggcgg gcgtgagcgg
      661 aaagttgtga tgatgatccg ctgaacaagc gtcggcaatg gcggtttttt gggccaacct
      721 tgggacgaga atgatgccag aagatccaaa accgggaatc gaagacttta gaaatggacc
      781 gccaggaatc gaaccggcgc agacttcaat tacagccatc atcggcaatt tcaattcgag
      841 cgcagccgag cgccctgttt tgctgaccag tgaggccgac ctaaacgaca aactgaacga
      901 caataccagt ccctcggccc aggcggtcgc ccagtttttc gtcaacggcg gccgggaatg
      961 ttgggtcgtt tgcgcgaagc cgactgagcc gaagttggac agggtcgagg cgctcaaatc
     1021 ggccatcgag agtctcgatc agattgacgc tccaaatcta ctcagcattg ctgcggcgtc
     1081 tgaacttgac gcgaccgagt gcgccgaagt catcgcgttc gccgcggaat tatgcaagac
     1141 ccgaaattct tttctcctgg tagatccgcc aacgaaacta cacactccag acgatatcct
     1201 gacctggctc gatggcaatc cagcccttcg catcgaaaat gctgcattgt attttccctg
     1261 ggtcagaatc gccgacaggt cgaatccggc gcagcaggta cgtcttccgc caagcacaac
     1321 gatggccgga gtaatcgcga gagtcgactt gaacagaggt gtctggaaag ctccggcggg
     1381 tcgggaagcc acattaatag ctgttgctgg cctcgactat tcgttatcag acaaggaaaa
     1441 cgatcgttta aatcggcacg gcgtgaattg cctgcgcaca ttccctgata gtgggccggt
     1501 gtgctgggga gcacgcaccg tttcactgaa taacgagtgg aggtacgtgc cggtgcggcg
     1561 cctggcattg tttattgaga gaagtatcta tcagagcacg cagtgggcca tctttgaacc
     1621 aaacgccgag gccctgtggg cgaacgtgcg ggcagtcatc gaggattttc tggtgggact
     1681 ttggcgacaa ggcgcactcg cgggcatcaa gccggaagag gcgttcttcg tcaaatgcga
     1741 ccggacaacg atgacgcaaa acgatctgga caacggccgg ctaattgccc tggttggaat
     1801 cgcgccgtta aagccctccg agtttgtgat catccgcatc gagttaatga cggcgggcgc
     1861 gagcgaaaag tgatcatgat cgtccgctga actggaaacc tctacacgcg gcccgctttt
     1921 tccgcgttct ccagttaact gcgttcaatg acatcgccgc acggttggat ctggcagaca
     1981 atgcgcgagt cccgaacttg atttgccgag gaatttggga taaacgcact caactcaaat
     2041 tgtcagacac caaagaggtc gcatgtcgga gcactttcag atcgttgttt cggcggaaaa
     2101 caattcctac ctgagttggc agt
//
LOCUS       DASUEX010000139         2453 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_599759, whole genome shotgun sequence.
ACCESSION   DASUEX010000139 DASUEX010000000
VERSION     DASUEX010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2453
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_599759"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /locus_tag="VFV61_02365"
     CDS             complement(<1..548)
                     /locus_tag="VFV61_02365"
                     /inference="COORDINATES: protein motif:HMM:NF013854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HEX4899343.1"
                     /translation="MPSICSTLLIGSRNPGKIREIKLALDELPLTLKSLDRVPNLSVP
                     KEFGETYAENAIIKATDYGRQTGMCVLADDSGLEVDWLEGEPGLHSARFGAGSDKDRV
                     ELLLAKLIGAANGDRRARFVSVVAIVHPTVGVLNVAEGECRGRINHAPLGNNGFGYDP
                     VFVPDGYQLTFAQLPNEVKARI"
     gene            complement(563..2197)
                     /locus_tag="VFV61_02370"
     CDS             complement(563..2197)
                     /locus_tag="VFV61_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899344.1"
                     /translation="MKTFRRILLVAALTLILAFVWIWLNRPQRADMSAAAPASALVYL
                     ESNSLMEIADGIAATSTWKLVQPLIGETKTAWPSSQTRRFIAFTGIGPTASVILARAQ
                     VAMVMIDLGAREEAETMTLKPEAALLIETHTSKRRIKSTVEQALQTFAERFYARPTLR
                     RTVVDGDEFLIWSAAENNRQIVAIIDDSLVIVANSDRAVKACLEARRGLRPSLNNEPE
                     LQRMKSRLSADGALAFGFVPPSHTPELLALATPVALGRAPGGAQIDGLVARSAAKILS
                     SAGWSAKLVEGAIEDHYFFALNSSVVSRMRPVFQPPQQPLKPTAFIPQALQSVTMYGF
                     KQPDQTWRELQTTLSSQLDTLSAVLLTSVLKSGLTPYGIDDPEKFLRLVGPEVMTLRL
                     KNEASSVLIAQVRDEPGLRALLQNPVAQTSDNEERNLTVHYQSGYVLMGSADDVQTCL
                     QATSEQKLKHFGSEPTATIVTYSDDSQRVADFISAIVRAQNTTAKVDGAVQMRQLVQL
                     PYAVTETSLSESGLDRRTRSPFGQFSALVPLLFPPN"
BASE COUNT          542 a          669 c          675 g          567 t
ORIGIN      
        1 cttatgcgcg ccttaacttc atttggaagc tgcgcaaacg tcagctggta gccatcagga
       61 acgaaaacgg gatcgtagcc gaatccattg ttaccaagcg gcgcatgatt aattcgtccg
      121 cggcattcac cttccgctac attaagcacg cctaccgtgg ggtggacgat tgccaccaca
      181 cttacgaagc gggcgcgccg atcaccattg gcagctccaa tgagttttgc aagcagcagc
      241 tcaacgcggt ccttatctga gccagcacca aatcttgccg agtgcagtcc gggttctccc
      301 tccagccaat ccacttccaa tcccgagtca tcggccagca cacacatacc tgtctggcgc
      361 ccataatccg tcgccttgat gatcgcgttc tcagcataag tctcgccgaa ttccttcggg
      421 acggacagat tagggacccg gtcgagggat ttcagggtca acggcagctc atccagagcg
      481 agtttaatct cccttatctt accgggattg cgactgccaa ttaacagggt ggaacaaatc
      541 gacggcatag aagaatcaaa atctaatttg gtggaaagag caagggcacc agagcgctaa
      601 actgcccgaa gggtgaacga gtgcggcgat ctagcccaga ttcactcagc gaagtttcgg
      661 tgactgcgta gggaagctgc accagctggc gcatctgtac cgcaccatct actttcgccg
      721 tcgtgttttg cgcacggacg attgcggaga taaagtccgc cacccgttgc gagtcgtcgc
      781 tgtaagtgac gattgttgcg gtgggctcgc ttccgaagtg tttcagcttt tgttcactcg
      841 tggcttgtag acaagtctgc acatcgtcgg cggaccccat caatacgtaa ccactctggt
      901 aatgcacagt gagattccgt tcttcattat ccgaggtttg ggccacggga ttctgcagca
      961 gtgctctcaa gccgggttcg tcgcgcactt gagcgatcaa gacggaactg gcttcgttct
     1021 tcaatcttag tgtcattacc tccggcccaa ccagccgcag aaacttctct ggatcgtcga
     1081 ttccatatgg cgttagtccg gatttaagaa ccgaagtaag cagcactgct gagagcgtgt
     1141 cgagctggga tgaaagagtt gtttgtaatt ctcgccaggt ctgatccggt tgtttgaagc
     1201 catacatggt gacagactgc agggcctggg gaataaaagc ggtaggcttt aatggttgct
     1261 gcggcggctg aaacaccgga cgcatgcgtg atactaccga agagttcagg gcgaaaaagt
     1321 agtggtcttc gattgcccct tccaccaact tcgcgctcca gcccgctgaa ctcagaatct
     1381 tcgcagcact cctggcgact aatccgtcaa tctgggcgcc gccaggtgct cttccgagcg
     1441 cgacaggagt cgccagtgcc agaagctccg gggtatggct cggtggtaca aaaccaaagg
     1501 ccaaagcacc atccgcactt aacctgcttt tcattcgttg cagttccggc tcgttattca
     1561 ggctgggtcg caaaccgcgc cgcgcttcaa ggcacgcttt cacggcgcga tcgctgttgg
     1621 caacaatcac gagggagtcg tcgatgatag ccacaatctg gcggttgttt tctgccgcgc
     1681 tccagattaa gaactcatca ccgtcgacta ctgtgcgtct gagcgtgggt cgggcgtaga
     1741 accgttccgc aaaagtttgc agtgcctgct caaccgtact cttgatgcgg cgcttggagg
     1801 tgtgggtttc aatcagcagt gccgcttccg gtttcagcgt catagtctct gcttcttctc
     1861 gcgcgcccag atcaatcatt accattgcga cttgtgcgcg cgcgagaatc acactcgcgg
     1921 ttggtccgat cccggtgaag gctatgaaac gccgtgtttg tgagctgggc caagcagtct
     1981 tggtttctcc gatcagtggc tgcactaact tccaggtgct ggttgcggct attccatcag
     2041 cgatttccat gaggctgttt gattcaagat agacgagggc actggcgggc gccgcagcag
     2101 acatgtctgc tcgttggggg cgatttagcc agatccaaac gaaagcaagg attagagtca
     2161 gagccgcgac gagcaaaatg cgacggaagg ttttcattga ctcgcatcgt agcgtctcag
     2221 cgcaaagtcc agcaagcagt gaggcaaaat gcgaaagttt attcgggtca gagagtacga
     2281 ttgataggcc agcggccgtg tggtactttc ttgcaaactt tcgggacccc ggacttaaag
     2341 aaacgctaat ccgtaatgac ataccgtgat ctatcagcgt cccccgctgc tgaggcggac
     2401 tttggtcagt tcctggcaca actccgtgat gagttgacca gcggccggcg gga
//
LOCUS       DASUEX010000140         2895 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_713711, whole genome shotgun sequence.
ACCESSION   DASUEX010000140 DASUEX010000000
VERSION     DASUEX010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2895
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_713711"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1463)
                     /locus_tag="VFV61_02375"
     CDS             complement(<1..1463)
                     /locus_tag="VFV61_02375"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEX4899345.1"
                     /translation="MHTRLEPKIFAIFREGYSRKQFYSDLAAGTTVGIVALPLAIAFA
                     IASGVKPEQGLFTAIVAGFVISLLGGSRAQISGPTGAFVVIIYGIVQQYGYDGLAVAT
                     LIAGGLLILMGVFRMGALLKFIPYPVTVGFTTGIALIIFSSQLGDFLGLNLASPPAGF
                     IEKLQAYRNHLFELNLTAVLIGVVSLLVIGIWPRITHRVPGMLIAIFVGTLVVKLFNL
                     PVATIGSRFGSVPNHLPLPHLPVISLSLIKQMFSPAVTIAFLAALESLLAAVVADGML
                     GTRHRSNMELVAQGFGNIGSVLFGGIPATGAIARTATNIKSGGRTPVAGMIHAVVLFL
                     ILLFFGKYAALIPMPTLAAILIVVAYNMSEWEEFTDLLRGPKSDAVVLLATFFLTVLI
                     DLTVAIQIGVLLATFLFMQRMSDATHITQVTETLVDQDETPTRDISKLEVPSGVEVFE
                     IYGSLFFGAIERFKDAMRSVEERPRVLILRMRQVQTI"
     gene            complement(1777..2433)
                     /locus_tag="VFV61_02380"
     CDS             complement(1777..2433)
                     /locus_tag="VFV61_02380"
                     /inference="COORDINATES: protein motif:HMM:NF018626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HEX4899346.1"
                     /translation="MAHSKYKLLLLIGVLAASGCQRQARIVSPKPAVPANVTIRPLPQ
                     NAPPQLKRMLDGAIAQAGVTTSYDPSYVALDYPGGDVPEKTGVCSDVVVRAFRKAGID
                     LQKEIHEDMKAARSDYPTKWGATSTDANIDHRRVLNLMAYFRRQGKSLTIIYDPTDYQ
                     PGDIVAWDLTSGIDHIGIVTNMLSDSGDRYLIVHNIGAGTRVEDVLFAWTIKGHYRFF
                     "
     gene            2526..2789
                     /locus_tag="VFV61_02385"
     CDS             2526..2789
                     /locus_tag="VFV61_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899347.1"
                     /translation="MNILQDIRYAFRMLIKRPGFTVIVVLTLALGIGANTTIFSAIDA
                     VLLNPLPYKDPERLMVVKIRASRSGERSSASPATSGIRPLRSR"
BASE COUNT          750 a          753 c          757 g          635 t
ORIGIN      
        1 tcaatcgtct gcacttgtcg catgcggagg atgagaaccc gcggacgttc ctccacgctg
       61 cgcatagcgt ctttgaaacg ttcgattgcc ccaaagaata atgaaccgta gatctcaaat
      121 acctcgacgc cagagggcac ctctaattta gagatatcgc gagtcggagt ttcgtcctga
      181 tcgactagcg tttcggtcac ctgggtaatg tgggtggcgt ctgacatacg ttgcatgaac
      241 aagaacgtcg ctaacagaac gccgatttgg attgccacag taaggtcaat cagaactgtc
      301 agaaagaatg tggccagcag cactacggcg tcactttttg gtccgcgcag aagatctgtg
      361 aattcctccc attcactcat gttgtaggcg acgacaatga gaatggcagc cagggtcggc
      421 atcggaatca gtgctgcata ctttccaaag aacaatagga tcagaaacaa cacgactgca
      481 tgaatcattc cggcaaccgg tgttcgacct ccgcttttta tgttcgtcgc agttcgagca
      541 atcgcgccgg tcgcgggaat tcctccgaac aaaaccgatc caatattgcc gaatccctgc
      601 gctactagtt ccatgttgga gcggtgtctg gtgccgagca ttccatcagc cacaaccgcc
      661 gcgagcagcg attcgagtgc cgccagaaag gcaatggtta ccgcaggcga gaacatctgc
      721 tttatgagtg acaacgagat tacggggagg tgcggcagcg gcagatgatt ggggacgctg
      781 ccgaaccggc tgcctattgt ggcaaccggc aaattaaaaa gcttaactac cagggtacca
      841 acgaagatcg caatcaacat ccccggcaca cgatgggtta tcctgggcca aattccgatc
      901 accaggagcg acaccacgcc gattagaacg gcggtaagat tgagctcaaa aaggtggttg
      961 cggtaggcct gcaacttttc aataaaacct gctggtggtg aggcgagatt gagaccaaga
     1021 aagtctccca gttgagacga gaaaatgatc agcgcaatcc cggtggtgaa gcctaccgtc
     1081 actggataag gaataaactt caacagggct cccatacgaa agacgcccat caagatcagc
     1141 agcccgccgg caattaaagt cgcaacagcc aacccatcgt aaccatattg ctgaacaatg
     1201 ccataaatga tgactacaaa agctcccgta ggtccgctta tctgcgcgcg agagcctccc
     1261 aatagggaaa tcacaaatcc agccacgatg gcagtgaaga gaccctgctc tggtttcaca
     1321 ccggaagcga tcgcaaatgc gatggcgagg ggcaaggcga cgataccaac agtcgtgccc
     1381 gccgccaggt cagaatagaa ttgttttcgt gaatatcctt cccgaaagat tgcgaagatt
     1441 ttcggctcca ggcgggtgtg catcgatagg tttctgtagt tattctaacg gggcctttta
     1501 tccaaaccta aaggcacgtt gttgtcttca agtacgaatg aatgatacca aactattcag
     1561 cactttgacg cgagctatgc gctcactgaa gattacgatt cgtggaacgc acaacaatca
     1621 ttgcgatcaa gctagtgttt gataaaaact gaagcgttac caaaaaacct ggtccaggat
     1681 ctcggccgcg gagaaactgc gtgacgtgtt ggaccaagca aagccaggac gtgcgtgcga
     1741 cactcacaag cgcacttacc gctgcggcat gagtgattaa aagaagcggt aatggccttt
     1801 gatcgtccag gcaaacagca catcttcaac tcgtgtgccg gcgccgatgt tgtgaacgat
     1861 gaggtaacga tctccggagt ctgacaacat gtttgtcacg ataccgatgt gatcgatgcc
     1921 gctggtcaga tcccaggcaa caatgtcacc aggttgatag tccgtggggt cgtaaataat
     1981 cgtcagcgat tttccctgac gtctgaagta agccatcagg ttcaaaacgc ggcgatgatc
     2041 gatattggca tcagtactag tcgcacccca ctttgtcggg tagtcggagc gcgcggcttt
     2101 catgtcttcg tgaatctctt tttgcagatc gataccggct ttacgaaacg cacgaaccac
     2161 cacgtccgaa caaacaccgg ttttttccgg cacgtctccg ccgggataat cgagtgcgac
     2221 gtaggatgga tcgtaactcg tcgtcacccc ggcctgcgcg attgcgccat caagcattcg
     2281 tttgagttga ggcggagcgt tctgcggaag tggccggata gtaacattgg ccggtacagc
     2341 aggtttggga ctgacgatgc gtgcctgtcg ctgacaaccg cttgctgcca acacaccgat
     2401 caggagtaag agtttgtatt ttgaatgggc cattggcatg tagctcttag acgccactgc
     2461 gcacacaagc gttccaaaag atattgaaac tgtgctgttg gcggtgctaa cttcagcacc
     2521 cgacaatgaa catcttacaa gacattcggt acgcctttcg gatgctaatc aagcggccag
     2581 gcttcacggt catcgtggtt ttgacactgg cgctgggcat cggcgctaac accacgatct
     2641 tcagcgccat cgatgccgtt ttgctaaacc cactacccta taaagatccg gaacgtttaa
     2701 tggtggtgaa gatccgggca agcaggtctg gcgagagatc gtcggcgtcg ccggcaacgt
     2761 caggcataag gcccttgaga tcgaggtaac gccggaagtc tattttccct acaaacagtt
     2821 gccgcgaaat ttcatgaacc tcgtcgtgcg cactgcttca gatccgccga gtatggttcc
     2881 cgcaatacgc aatca
//
LOCUS       DASUEX010000141         2528 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1515763, whole genome shotgun sequence.
ACCESSION   DASUEX010000141 DASUEX010000000
VERSION     DASUEX010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1515763"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..696
                     /locus_tag="VFV61_02390"
     CDS             <1..696
                     /locus_tag="VFV61_02390"
                     /inference="COORDINATES: protein motif:HMM:NF017970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HEX4899348.1"
                     /translation="SVRIWVLDEDVKPVTLAELKPGDFFGELAVLDRGARSTNATAIV
                     DSTLHRLSSDDFQKFLLEHPDVAIDVICEIGARMRQTNALVSQRVTRNINEQIEQSST
                     VGQKVADKVASFGGSWTFIFIYVGFLLSWIIFNSLVLLHYGSREGGAQFDPYPYILLN
                     LMLSMTAALQAPIIMMSQNRAAEKDRLAAEQDFRVNLKSELMLEDLTRKGRERDEQLN
                     ELIALVKDLKEKS"
     gene            complement(789..2039)
                     /locus_tag="VFV61_02395"
     CDS             complement(789..2039)
                     /locus_tag="VFV61_02395"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX4899349.1"
                     /translation="MASSNLISHAGASSQGQDARPRRSGTPTPTPVPRSGVMQPQATA
                     TQTPGPVTTQTPAARPTATPPPSPTPYDRIAPTLGEPPPPPVLRAKPSPTPEEEIDEG
                     SIVRINTALVPLNVRVIDRNNRPINNVRQEDFQVFEDGVPQPIESFSREEVPISYGLA
                     VDTSGSLRSQLLSVIDAGKTIINSNKPEDETFLARFISKDKIETVQDFTSNRDLLMDG
                     LDSLYVEGGQTAVIDAVYLTAEHVAEYKKGDESDRRRRALIVITDGEDRDSFYKQEQL
                     FAKLREEDVQIFVVGFVNELDKEGGFIRKSPRERAVNLLTKFATETGGRAFFPQSLSE
                     LPQIANDIVRDLRTQYVVTYNPTNKERDGTYRAIKVVVADASGEKRIALTRSGRLATK
                     ETGVVPKPPPVKMPATKVGAGSRP"
BASE COUNT          532 a          672 c          722 g          602 t
ORIGIN      
        1 tcagttcgga tttgggtgct cgacgaagat gttaagccag tgacgctggc cgagctgaag
       61 cctggcgact ttttcggcga gttggctgtc ctggatcgag gcgcacgctc aactaacgct
      121 accgccatcg tggattcaac gctgcaccgg ttaagcagtg acgactttca aaagtttttg
      181 ttggagcatc ctgatgtagc gatcgacgtc atctgtgaaa tcggcgcgcg gatgcggcag
      241 acaaatgcac tggtttccca gcgagtcact cgtaacatca acgaacagat tgagcagagc
      301 tcgacggtgg ggcagaaagt agcggacaag gttgcttcat tcggcggttc gtggacgttc
      361 atattcatct atgtgggatt tctcctgagc tggataatct tcaactcgct cgtgctcctc
      421 cactatggca gccgagaggg tggagctcaa tttgatccct atccttatat tttgctgaac
      481 ctgatgctct cgatgactgc ggctcttcaa gccccgatca tcatgatgtc gcaaaaccgc
      541 gctgctgaaa aagacaggtt ggcggcggag caggacttta gggtgaacct gaaaagcgaa
      601 ttgatgctgg aagacttgac gcgcaagggt cgcgagcgtg acgagcagtt gaacgaattg
      661 atcgcgctgg tgaaggatct aaaagagaaa tcttaagtgg gtcgccacat tgcagggatt
      721 agagaccacg ctaatgaaaa agggcacgcg ctggcgtgcc cttggcattt ttgacgtggc
      781 cctccaggct acggtcgtga tccggcgccg actttcgtgg ccggcatttt gactggaggt
      841 ggctttggta ccacgccagt ttctttcgtg gccaaacgtc cactgcgggt cagcgcgatc
      901 cgtttttcgc cgctcgcgtc ggcaactacg accttaattg cacggtaggt cccgtcacgt
      961 tctttattcg tgggattgta ggtgactaca tattgcgttc tcaggtctcg gacgatgtcg
     1021 ttggcaatct gaggcaactc ggaaagtgat tgcggaaaaa acgctctgcc gccggtctca
     1081 gtcgcaaatt tcgtcagcag gttaaccgct cgctcgcgcg ggctcttgcg aataaatccg
     1141 ccctccttat ccagttcatt gacaaagccg acgacgaaga tctgcacgtc ttcttctcgc
     1201 aactttgcaa agagctgttc ctgcttgtag aagctgtcgc gatcttctcc gtcggtgatt
     1261 acaatcagcg cccgccgcct gcgatcactt tcgtccccct tcttgtactc ggccacatgt
     1321 tcggccgtga gatacacggc atcgatcacc gcggtctggc caccttcgac gtaaagactg
     1381 tcaagaccgt ccatcaagag gtctcgattt gaagtaaagt cctgaactgt ttcgatcttg
     1441 tccttgctga taaaacgcgc gagaaaggtt tcgtcctccg gcttattgct attaattatc
     1501 gtcttacctg catcaattac gcttagcaac tgcgagcgca aggaccccga agtgtcaacg
     1561 gccagtccgt aactgattgg cacctcttcc cgagaaaaag actcaattgg ttgcggcacg
     1621 ccatcttcga aaacctggaa gtcttcctgc cgtacgttgt tgatgggccg gttatttcga
     1681 tcgatcactc gaacgttcag tgggaccaac gcagtgttga ttcgaacgat gctcccttcg
     1741 tcgatctctt cttccggcgt gggacttggc tttgctcgca agaccggcgg tggtggcggc
     1801 tcacccagag tgggtgcaat gcggtcgtac ggcgtcggac tcggcggtgg cgtcgcagtg
     1861 ggacgggctg ccggtgtctg cgtcgtcacc ggtcctggtg tctgagtcgc agttgcctgc
     1921 ggttgcatca cgccagagcg aggaaccggg gtcggagttg gtgtgccgct gcgtcgtggt
     1981 cttgcgtcct gtccctggct actggcgcct gcgtgcgaaa tcaagtttga gctcgccatc
     2041 aggccgagca gtagaacaaa tgtgcccacg aaccaaaagg cccgggtccg agagctaggt
     2101 aacaaagtct cttgctgtcg catcaacgat ctattccttc aacatgaaaa ccagggtgcc
     2161 gaaacatgag aagcctcgga tcaaaaacta gtctagcctt tcaggccgcc catgcgaagc
     2221 gctccgcacc ccgtcattgg tcggggtcgg agcgctgaac cgaattctaa acggtacgct
     2281 cgcatccgtc agggctgggg caagtgccgg gggtctcact aaggagatcc actgcgcaca
     2341 ttgaaagatg ctgcgggaga gaaaatgcgt tgccggataa ggcgaaaaaa cggttatcgc
     2401 accggcggca gggcgtagta accctctttc gagcgcacga acaagcgtgg cagaccgcgt
     2461 ggaggtgtga cctttacctt cactttgtgc catttgccgt cgttggagac attgcttggt
     2521 gtgtagcc
//
LOCUS       DASUEX010000142         3030 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_357250, whole genome shotgun sequence.
ACCESSION   DASUEX010000142 DASUEX010000000
VERSION     DASUEX010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3030
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357250"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="VFV61_02400"
     CDS             <1..227
                     /locus_tag="VFV61_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899350.1"
                     /translation="ATVESQADSPARAAEMAKGYSGLLAVGQYLKRGRDEEQLYKSTK
                     VTAEGNQILVNFSMPRQTASEMLKKQLPAG"
     gene            337..>3030
                     /gene="secA"
                     /locus_tag="VFV61_02405"
     CDS             337..>3030
                     /gene="secA"
                     /locus_tag="VFV61_02405"
                     /inference="COORDINATES: protein motif:HMM:NF009538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEX4899351.1"
                     /translation="MIDKFLTKVFGSSNQRFLKSIQPIVDQINALEPSVKKMTDDELR
                     ARTAAFKEQAQRAVAGARDKEERKRLEREMLDEILPEAFAIVREASVRTTGMRHFDVQ
                     LIGGIVLHQGRIAEMRTGEGKTLVATLPAYLNALTGRGGVHVITVNDYLASRDAEWMG
                     QIHRFLGLEVGCIQNDMDDFERQAAYAADITYGTNNEFGFDYLRDNMKFDLATCVQRG
                     HYFAIVDEVDSILIDEARTPLIISGPSDEATDKYYKADAIIPQLKRGEDNEGVKTGDY
                     VVDEKAHTAVLTEDGVDKAERLLGVGNLYDPSNMELLHCVEQALKAHTLYRLDHQYVV
                     QDGEVIIVDDFTGRLMKGRRWSDGLHQAVEAKEGVRIEKENQTLATITLQNYFRLYEK
                     LSGMTGTAETEAAEFQSTYKLDVIVIPTHMPMVRKDQSDVIYRTLPEKWDAVIEEIKD
                     CYDRGQPTLVGTVSVENSELISRRLQREAVPHNVLNAKYHEREAEIVAQAGRKGAVTI
                     ATNMAGRGTDILLGGNPDFMAREFLKKDEIDPDEATEVQWDEAFRKAKRIVESEHQEV
                     VTIGGLHIVGTERHESRRIDNQLRGRAGRQGDPGSSRFFLSLEDDLMRIFAGDKVKAL
                     MQRLGMEQGVAIESKMVSKRIESAQKSVEGRNFEARKHLLEYDDVMNKQRETIYGLRR
                     QLMEEPDQREYLLGDPPKSGVAYDLLSDITKQYLNPDVSPEDWNSDNYKLQIKTIYDL
                     DIDAEKIDLESLSSEEVLERVWEKLKTKYAEKEQQIGPEAMRTYERIIMLNIIDAQWK
                     DHLLSLDHLKQGIGLVGYGQKDPLVEYKKQSFDLFQEMLDRIDTTTIRSLFNLQVVAE
                     QPPEALRQRRLPRRQTSLTFTGPNQGAAPAGEEAGKIKTVVR"
BASE COUNT          781 a          753 c          873 g          623 t
ORIGIN      
        1 ctgccaccgt agagtcacag gcggattcac cggcgcgagc ggccgaaatg gccaaagggt
       61 acagcggctt gttggctgtg gggcaatact taaagcgcgg tcgcgacgaa gagcagctct
      121 acaaaagcac caaagtcacg gccgagggca atcagatcct ggtgaacttt tcgatgccgc
      181 gacaaacggc cagcgagatg ctgaaaaagc agcttccggc aggttagccg tgcggggcta
      241 ttcgcccgtt tgcctcactc caaccactcc actacaatca ttaattgccc ttggaagagc
      301 aaattagctt tcaattgata gagactataa cgacttatga tcgacaaatt tctaactaag
      361 gtattcggca gcagcaacca gcgattcctc aagtccattc aaccaatcgt tgaccagatc
      421 aacgcgctcg agccatctgt caagaagatg actgatgacg agcttcgtgc tcgtacggct
      481 gctttcaaag agcaggcgca gcgcgccgtt gctggggctc gcgataagga agaacgcaag
      541 cggctcgaac gggaaatgct ggatgagatc ctgccggaag cgtttgcaat agttcgtgaa
      601 gcaagtgtgc gtaccaccgg gatgcgccat ttcgacgtgc agttgattgg cggcatcgtc
      661 ctgcaccagg gaaggattgc tgagatgcgc actggtgagg gcaaaacact ggtggcaact
      721 ctgcccgcat acttgaacgc cctgaccggg cgcggcggtg ttcacgtgat cactgtcaac
      781 gattatcttg catcgcgcga cgcagagtgg atggggcaga ttcaccgctt tctggggttg
      841 gaggtcggct gtatccagaa cgatatggac gattttgagc gccaggcggc atatgctgcg
      901 gacattacct acggtactaa taacgagttt gggtttgact atctgcgtga caacatgaag
      961 tttgacctcg ccacgtgtgt gcagcgcggc cactacttcg caatcgtcga cgaggtggat
     1021 tccatcctga tcgacgaagc ccgtactccg ctgatcattt ccggcccctc cgacgaagca
     1081 accgataagt actacaaagc cgatgcgatc attccccaac tcaagcgcgg cgaagacaat
     1141 gaaggggtga agactggcga ctatgtggtg gatgaaaaag cacacacggc ggtactgacc
     1201 gaagacggag ttgataaagc tgagcggcta ttaggggtgg gcaacctcta tgatccttca
     1261 aacatggaac tgctccattg cgtggagcag gccctgaagg cgcacacact ttatcgcctg
     1321 gaccaccaat acgtcgtaca ggacggtgaa gtaattatcg ttgacgactt tactggccgt
     1381 ttgatgaaag ggcggcgctg gtcagatggt ttgcatcagg cagtcgaagc caaagagggt
     1441 gtcaggattg aaaaggaaaa tcagaccctt gccacgataa ccctgcaaaa ctattttcga
     1501 ctttacgaga agctgtccgg gatgacaggt accgctgaaa cggaagctgc cgagtttcaa
     1561 tccacctaca aattggatgt gatcgtgatc cccacgcaca tgccgatggt gcgcaaggat
     1621 caatccgatg tgatctatcg cacgctgccg gagaagtggg atgcagtgat tgaagagatc
     1681 aaggactgtt atgaccgcgg ccagccgacg ctcgtaggta ctgtttcagt ggaaaattct
     1741 gagttgattt cacgacggct gcaacgcgaa gcggtgccgc ataatgttct gaatgcgaaa
     1801 tatcacgaac gcgaagccga gatcgtggcc caggcaggcc gcaaaggcgc cgtgactatt
     1861 gctaccaaca tggctggtcg tggtaccgac atcctgcttg gcggtaatcc ggatttcatg
     1921 gcgcgtgaat tcctaaagaa ggacgaaata gatcctgacg aagcgacgga ggtgcagtgg
     1981 gacgaggcat tccgcaaggc caaaagaatc gttgaaagcg agcatcagga ggtagttact
     2041 atcggcgggc tgcacatcgt tggtactgaa cggcatgaat cacggcgcat tgataatcag
     2101 ttgcgcggac gtgccggccg gcaaggtgac cccggttcgt cgcggttctt tctttctttg
     2161 gaagacgacc taatgaggat ctttgcgggc gacaaggtaa aagccctgat gcaacggctc
     2221 gggatggagc agggtgtcgc gatcgaatcg aagatggttt caaaacggat tgagtcagcg
     2281 caaaaatccg ttgaagggcg caacttcgaa gcacgcaaac atctgctcga atacgacgac
     2341 gtgatgaata agcagcgcga gaccatctac ggattgcgcc gccagttgat ggaagaacca
     2401 gatcagcgcg aatacttgtt gggcgatcca ccgaaatcgg gtgtggccta cgatctactg
     2461 tccgatatca cgaaacagta cttgaaccct gatgtgtcgc ctgaggactg gaactccgat
     2521 aactacaagc tacagatcaa gaccatctat gacctggaca tcgatgcaga aaaaatcgat
     2581 ctcgaaagtc tctcttccga ggaagtgctt gagagagtct gggaaaagct gaagaccaag
     2641 tatgcggaga aggaacagca gattgggccc gaggcgatgc gcacctatga acgcatcatc
     2701 atgctcaaca tcatcgatgc gcaatggaag gatcacctgc tttctttgga ccacctaaag
     2761 caggggattg ggttggtcgg gtacggccag aaagacccgc ttgttgagta caagaagcag
     2821 agctttgacc tcttccagga gatgctcgat cgaattgata ccactaccat caggtcgctc
     2881 ttcaatcttc aggtggttgc cgagcagccg cccgaagcgt tgcgtcagag acggcttccg
     2941 cgtcgccaaa cgtcgttgac ttttacgggg ccgaaccagg gagcggcacc tgccggagaa
     3001 gaagcgggta agatcaagac tgtcgttcgt
//
LOCUS       DASUEX010000143         2736 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1395181, whole genome shotgun sequence.
ACCESSION   DASUEX010000143 DASUEX010000000
VERSION     DASUEX010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1395181"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="VFV61_02410"
     CDS             <1..216
                     /locus_tag="VFV61_02410"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX4899352.1"
                     /translation="YLIKHEGQVVKRKSMLEEVWGLHEDTDTRAIDNFIVRLRRYIET
                     EPSRPKHLLTVRGLGYRFVSGDEKGHG"
     gene            complement(399..1346)
                     /locus_tag="VFV61_02415"
     CDS             complement(399..1346)
                     /locus_tag="VFV61_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899353.1"
                     /translation="MGYNNIDAFGNGWLAFELNVLRRLKFKSVALPLSGEPYLGVQLK
                     RWDVRVVANDPFTWASTKATALIENSALKLDEADYEIVLQDTYVPRDELDNVALLKWF
                     NESDAWWFDNLRANAEKLQDPHKRAIALTLGMMVGDYVLSFDTQTLGMREPLSLPKVF
                     RQMEELLPQPYDNALRNKSTNQDLRSFVAERQHTDLLFLRLPAPVVQVEPSRGPSSWR
                     EEWLQGGDSFWNEFDLKRAGRLGAPVQSKQQYLGLVEDLLRTASHIPSWAIAHTENGF
                     ISGEELMETVGRIRRVEAVYVKDFSDLLGVKASIITAGT"
     gene            complement(1370..2029)
                     /locus_tag="VFV61_02420"
     CDS             complement(1370..2029)
                     /locus_tag="VFV61_02420"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX4899354.1"
                     /translation="MSTMTTNDKKHVDAFTQLASATDEFEQYKSPHAVRIVELADEIA
                     QHFLLASRDRRSLRAAALLHDLGEAAMERDYIQRAAALSADERVDLTRHPVIGEQEAA
                     RVGADRATTLLVRWHHEWWNGSGYPDGLRGDEIPLAGRILRLADSYVSLTASRPFRAA
                     YSAAVARQMIIERAGIEFDPRVVQTLLQLELTDHVEPAEAVEPVKTGYESKELFSSFM
                     K"
     gene            complement(2081..>2736)
                     /locus_tag="VFV61_02425"
     CDS             complement(2081..>2736)
                     /locus_tag="VFV61_02425"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX4899355.1"
                     /translation="EIKNSYYALARRYHPDRFHMQAGTPLHASVESAFAKIAQAYVTL
                     ADPSQRTAYNAKLAAQERIRSSTMEIPKQTEQDRSRANAANREQGEKAADDFSRAESN
                     FQEGFIALQQGQTKAAIVSLAAAARLAPGEARFRAYYGRALASGEGTRRLAEAELQAA
                     LKLDPANSTYRLMLAELCFDLGFYRRAEAELKRVLTAEPNNPGGHKLLRRLEATTTS"
BASE COUNT          603 a          777 c          720 g          636 t
ORIGIN      
        1 tatctgatca aacatgaggg acaggtggtg aagcgcaagt cgatgctgga agaagtttgg
       61 ggactccacg aagataccga cacgcgcgca attgacaact tcattgttcg gctcaggcgc
      121 tacatcgaaa ccgagccgtc acgtccgaaa cacttgctga cggttcgagg attgggatac
      181 cggtttgtga gcggggatga gaagggtcac gggtaaaaac gagcttcgaa cttggttctt
      241 tgaactttgt ttgctgggtg aaacttgacc ggcagatgtc aaggctgagc acttgcagca
      301 aagaactaaa cacaaagtac taagctcatt ttattctctc ggcgaactct gcggtccttc
      361 gcggcctggc ggttttagtt tctgcaaatg gaaaaccgct aggttccagc ggtgatgatc
      421 gatgctttca ctcccagcaa gtcggaaaag tcttttacgt agaccgcctc cactcttctg
      481 atacgcccga ctgtttccat taactcttcg cctgagatga acccattctc cgtatgggca
      541 atcgcccatg aaggaatgtg tgaggctgtg cgcaacaaat cttcaaccag tccgagatac
      601 tgttgcttgc tttgcaccgg agcgccgagt cgacctgcgc gcttcaggtc aaactcattc
      661 caaaaactat cgccgccctg gagccattct tcgcgccaac tcgacggccc acgcgagggc
      721 tcaacctgca ccaccggcgc aggcaatctc aaaaaaagca gatcggtatg ttgccgctcg
      781 gcaacaaagc tacgcagatc ctggttcgtg ctcttgtttc ttagagcgtt gtcgtagggc
      841 tgtggcagta attcctccat ctgtcgaaag accttgggca gggacagtgg ctcccgcatc
      901 cccagcgtct gcgtgtcaaa ggaaagaaca tagtctccga ccatcatccc cagggtgaga
      961 gcaatagcgc gtttgtgcgg atcttgcagt ttctcggcgt tggcgcgcag attgtcaaac
     1021 caccaggcat cgctttcatt aaaccatttc agaagggcaa cattgtccag ctcatctcgc
     1081 ggcacgtagg tgtcctgaag gacgatttcg tagtcagctt catccagttt taaagcggag
     1141 ttttctatta gcgcagtcgc tttggtagag gcccaggtga aaggatcatt cgcaacgacg
     1201 cgcacgtccc aacgcttcaa ctgcacaccc aggtacggtt cgccacttaa cggcagggcc
     1261 accgatttga atttcaagcg gcgcagaacg ttgagctcaa acgctaacca gccgtttcca
     1321 aatgcatcga tgttgttgta acccactttt ctgttgacct tcctacgcct tacttcataa
     1381 acgacgagaa cagttcttta ctctcgtatc cggtcttaac aggctcgact gcttcggccg
     1441 gttctacgtg atcggtgagt tcaagctgca gcaacgtctg caccactcgc ggatcgaatt
     1501 cgattcccgc tcgctcaata atcatttgtc gggcaacagc cgccgagtat gccgcccgaa
     1561 aggggcggct agccgtcaac gatacgtatg aatccgccag ccgcagaatc cgtcccgcga
     1621 gcgggatctc atcacctcgg agcccgtcgg ggtagccgct gccgttccac cactcatgat
     1681 gccagcgaac tagcagcgta gtagcgcgat cagcaccgac gcgcgcggcc tcctgttcgc
     1741 cgataactgg atgccgcgtc agatcgacac gttcatcagc actcaacgcc gcggcgcgct
     1801 gaatgtaatc tctttccatt gccgcttcac caagatcgtg cagcaacgct gcggcccgta
     1861 gcgacctgcg atcccgactt gccagcaaaa aatgctgcgc gatttcgtcg gcgagttcca
     1921 caatacgcac ggcgtgggga ctcttgtatt gctcaaactc gtcagtggcc gacgccagtt
     1981 gagtaaaggc gtcgacgtgc tttttgtcat ttgttgtcat ggtagacatt agtaacgaga
     2041 ttctacttgg ctgaacagct acaaccaacc gttcctaaca ctaagacgtc gtcgtggctt
     2101 caagacggcg taacagttta tgcccaccag ggttatttgg ctccgcagtc agcacgcgtt
     2161 tcagttcggc ttcggcccga cggtagaagc ccaaatcaaa acagagttcg gccagcatca
     2221 agcgatacgt tgagtttgcc ggatcaagtt tgagcgccgc ttgcagctct gcctcagcga
     2281 ggcgccgcgt gccttctcct gatgcgagtg cccgtccata gtaagcacga aaccgtgcct
     2341 cgccgggcgc caaacgtgcg gcggcggcca aactgacgat cgctgctttg gtttgccctt
     2401 gctgcaaagc gataaaccct tcctgaaaat tgctctcggc gcgcgaaaag tcatcggccg
     2461 ccttctcacc ctgctctcta ttagctgcgt tagcgcgtga acgatcctgt tcagtctgct
     2521 tcgggatctc catagtcgag ctgcggattc gttcctgggc cgcaagcttg gcgttataag
     2581 cggtgcgttg gctgggatcc gcgagggtca cgtatgcttg agcgattttg gcgaaagcgg
     2641 actcgacgct tgcgtgtaac ggcgtgcccg cctgcatatg aaagcgatcc ggatgataac
     2701 gacgcgccaa cgcgtaatag ctgttcttga tttcct
//
LOCUS       DASUEX010000144         2248 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_714484, whole genome shotgun sequence.
ACCESSION   DASUEX010000144 DASUEX010000000
VERSION     DASUEX010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_714484"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..884
                     /locus_tag="VFV61_02430"
     CDS             <1..884
                     /locus_tag="VFV61_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899356.1"
                     /translation="LPIHVGDRYPGPIETIEGIKRAVTSAVGRSPTDVAPVCCDAKGN
                     YMIYVGLPGASIKHTKLNPVPTDKTHFPPKIIELYEQAMAASSAAVLRNNAIEDRSQG
                     YALSTGDSALRAKQLEVRAYALQHEKLIRAVLDSSSEAQQRIVAAYLLGYAGQSKQQI
                     AALVRASYDADETVRNNATRALGVLAESSIRVAAQIPPDRFIEMLSSGSWSDRNKAGL
                     VLDNLTRSRNPKLLAQLRSEALVSLIEMARWRNAGHASTARLLLARMAGIEEERAQQL
                     ANEDNADEIINALSKAP"
     gene            complement(894..>2248)
                     /locus_tag="VFV61_02435"
     CDS             complement(894..>2248)
                     /locus_tag="VFV61_02435"
                     /inference="COORDINATES: protein motif:HMM:NF019285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HEX4899357.1"
                     /translation="ALLSSRILPPGNKKPRRGWQEMIHHAVSVLGFIVFAGIAWSLSS
                     NRRRIAWRTIVWGVALQVLIGLIIFRLPASHRILLWLNDAVLVLLNASKSGSVFLFGP
                     LAASPGEAGSIGFILVFQVLPVVIFFAAFTSLLYHLRVLQVFVRLFARLFHRTMKISG
                     AESLSSAANIFVGIESALVVRPYLERMTRSELLLILTTGMATVASSTLGVYVGFLTGV
                     FPQIAGHLISASVLAIPACVVISKLLLPETETPETLTAVPPEDESTRSKNLISAIIEG
                     AMEGLKLAAGVATLLIAMLGIVALFDKILAVGSSWLGMSQPLSIVRILGWCFYPFAFL
                     LGVQSSDVPIAARLLGERVILTEVFSYNHLAQLISSGQITDPRTVVILSYALCGFAHV
                     AAMAIFVGGTAALAPSRRDDLASLGLRALLAATLATLMSGCIAGMFSGGEGILLSRPG
                     "
BASE COUNT          580 a          599 c          601 g          468 t
ORIGIN      
        1 cattaccaat tcacgtgggt gatcgatacc cgggacccat tgaaacaatt gagggaatca
       61 aacgagctgt gacctccgct gtcggtcgct cgccaacgga tgtggctccg gtatgttgcg
      121 atgctaaagg caactacatg atctacgttg gcctccccgg cgcctcgata aaacacacaa
      181 aactcaaccc ggtcccaaca gataagactc attttccgcc aaagatcata gagctctacg
      241 agcaggcaat ggcggcctct agcgcagctg ttttgaggaa caatgccatt gaagatcgtt
      301 cgcaaggcta tgcgctctcg actggtgatt cggccttacg tgccaaacaa ctggaagttc
      361 gtgcttacgc tctgcaacat gaaaagttaa tccgtgcggt gttggattcg tcgagcgagg
      421 cacaacaacg tatcgtcgca gcatatctgc ttggctatgc tggtcaatct aagcaacaga
      481 tcgctgctct ggtgcgcgcc agttatgacg cggatgagac agttagaaac aatgcaacaa
      541 gggctttagg cgttctcgca gaatccagca ttagagtggc cgcgcaaatt ccaccggaca
      601 gattcattga aatgctcagt tcgggttcat ggagtgatcg taacaaagcg ggattggtac
      661 tcgacaactt gacgagaagc agaaatccaa agctgctcgc ccaattacgt tccgaagcat
      721 tggtgtcact catcgagatg gctcgttggc gaaatgctgg acacgcttcc actgcacgac
      781 ttctgctcgc acgcatggcg ggaattgagg aagaacgtgc acagcaactg gccaacgagg
      841 ataacgccga cgaaataatc aatgcgttat cgaaggcacc atagggtcat tgcctagcct
      901 ggtctggaca gcaatattcc ttcgccacca ctaaacatgc ccgctatgca gccgctcatg
      961 agtgttgcca atgtcgctgc cagcaaggct cgtaagccga ggctggcgag atcgtcgcga
     1021 cgcgacggtg caagtgctgc agtacctcct acaaatattg ccatcgcggc cacgtgcgcg
     1081 aatccgcaga gcgcgtaact gaggatcacc acggtccgag gatcggtgat ctgtccgctg
     1141 gagattaact gtgcgagatg gttgtaggag aagacctctg tcagtatgac tcgctcgccc
     1201 aacaagcgag ctgcgatcgg tacatcgctc gactgcactc caagcagaaa agcgaaagga
     1261 tagaaacacc agcccaggat tctgacaatc gaaagtggtt gactcatccc taaccacgaa
     1321 cttccaacgg ctagtatttt gtcgaacagg gccacgattc ccagcattgc gatcagcaat
     1381 gtcgcaactc cggcggccag tttcagtcct tccatcgctc cttcgatgat tgcgctgatc
     1441 agattctttg atcgcgtcga ttcatcttcc gggggaacgg ctgtgagtgt ctcgggcgtt
     1501 tcagtctcag gcagcaggag ttttgatatg actacgcaag cagggatcgc taagaccgaa
     1561 gcagagatca ggtgcccggc gatctgtgga aacactccgg tgagaaagcc gacgtagact
     1621 cccagcgtac tcgatgccac cgttgccatg ccggtagtca aaatcagcaa caactccgaa
     1681 cgcgtcattc tttccagata cggtcgcacc acgagcgccg actcaattcc cacaaagatg
     1741 ttcgccgcac tggagagtga ttcagcgccg ctgattttca tcgttcggtg gaagagcctg
     1801 gcgaagaggc gcacgaatac ctgcagcacg cgcaggtgat agagcaggga ggtgaatgct
     1861 gcgaagaaaa tgaccacggg aagaacttga aagacgagga tgaagccgat ggatcctgct
     1921 tctccgggac ttgcggccaa tggtccaaac aggaacaccg aaccgctctt tgacgcgttt
     1981 agtaagacga gcacggcgtc gttcagccag agtaagattc gatgggaagc gggtaaacga
     2041 aagataatca gaccgatcag gacttgcaga gcgactcccc acacgatcgt tctccaggca
     2101 atccttcgtc gattacttga aagtgaccaa gcgattcccg cgaacactat gaagcccagg
     2161 actgacaccg catggtgaat catctcttgc catcctcgtc ttggtttctt attgccgggc
     2221 ggtaagatac gagatgaaag aagcgcga
//
LOCUS       DASUEX010000145         4440 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_829263, whole genome shotgun sequence.
ACCESSION   DASUEX010000145 DASUEX010000000
VERSION     DASUEX010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4440
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_829263"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            410..1051
                     /locus_tag="VFV61_02440"
     CDS             410..1051
                     /locus_tag="VFV61_02440"
                     /inference="COORDINATES: protein motif:HMM:NF012523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatA-like O-acetyltransferase"
                     /protein_id="HEX4899358.1"
                     /translation="MPNYLDVSTWARRDLFEFFRTYENPYFNICGQLDVSSLLDLLQS
                     KPDVSPTLTYHYLALRVANEIEPFKYRLRDDQILIHDVIHGGTTVLLPNESFTFAYFD
                     YQIAFDKFIKDAQASVAAVKRGDTPFKPLDGDDRIHFTVLPWVSFTSFAHARNKTRAI
                     SVPKIAFGKFIRNNERTYLPTSVEVHHAMMDGLHVGRFFNRMEEMLAEPSAYL"
     gene            1150..2895
                     /locus_tag="VFV61_02445"
     CDS             1150..2895
                     /locus_tag="VFV61_02445"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEX4899359.1"
                     /translation="MRWSQYFIPTLREEPADAEVVSHKLLLRAGLVRQLGAGLYSILP
                     LGQRVALKIIEILREEMNGIGAQEFYLPALNPREIWDESGRWDVMGDNMFRLKDRKGA
                     DLCLGMTHEEVFTSIARSDLNSYKQLPQVWYQIQVKFRDEVRPKSGVLRVRQFIMKDA
                     YSFDADMNGLDKSFNDQRAAYQRIFDRCGLQYVIVEASSGSMGGSASNEFMVKTDAGE
                     DMIATCASCGYAANLEKATSRLDEVRDGAGLDFPSEFPTPGVRTIDDLANFAGGAAAE
                     SQIKTLVYVDSGASAYYLALLRGDHQLHETKLFDALGANARAATGEEIQSLLGANPGS
                     LGAVGAKKKASELQPGATVRIVADLALRSRRNMTTGANIDDYHLRGIDVGRDIKIDQW
                     ADLRTVNSGEKCPQCENGTLEVAKALEIGHIFKLGTKYSESMGANVLTQEGKQVPIVM
                     GSYGIGVERIMASAIELHHDDDGIIWPKSIAPFDVIVTITNMKQDEVRNAGESLYKEL
                     HRAGLDVLLDDRDERAGVKFKDADLIGIPYRVTVGKKVSEGLVELFDRRAKTTEEVKL
                     ADISGRMQKLALAGL"
     gene            2914..4341
                     /locus_tag="VFV61_02450"
     CDS             2914..4341
                     /locus_tag="VFV61_02450"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX4899360.1"
                     /translation="MKSLCLSLVLLITSVLPCPAQELLPDLVRRIKPSAVAIETFDAR
                     GEKIARGSGFFIDFDRVVTNRHVIEAAYRAEVHSYTGIVMIVKGVLAVDAEGDLALLK
                     VEAPPNQVRPLPLDRTSPQEGESVVVIGNPFGLEGSVTNGIVSAVRDIPTFGRIIQIT
                     APISPGSSGSPVVNMQGQVIGVATLQITGGQSVNFAIPSERISQLQTATLMTLPELVA
                     SSGRNKRAKAVQFFRDGLSFLSRDDCERALPYFEKAVDSDSNYAEAWAQSGFCKEKLG
                     RHAEAIEASKKAVSLRPAAESYFNIGLANYYLKQYRESAEAYRAAIRLDPYNSADAYY
                     ALGLVYRDWGKADDEIQAYKQAIKLRPDYTSAYERLGSRYFRSRKYSEAAEIYKQLAS
                     LKPGDASANNSLGETYLEMSKFNEALDNFRQAIRLKPDYGRAYFNLGKCFLAMGNRDA
                     ALEQYNILQNLDQDWAEKLGNLINP"
BASE COUNT         1042 a         1173 c         1198 g         1027 t
ORIGIN      
        1 gcaacttcct cggcgacctt acgagcgtaa tccgggtaat cgacggaaga ctccgagagc
       61 gttcccaagt ccgccacttc gattcccaac tcatcgagca gcgacttgag tctctccttt
      121 gcggaaaagc cggcgtggtc actggcaagc gcaatcttca cgtgttcaac ctcatgtaaa
      181 aagaattagc cgaagagggc gtcgatgtta caacacgcga cgtaagttgt cagttgttat
      241 ggagtgcgcc ggcaaagcga agtcccgacg cgtcgggaac ggcgctttgg ttgctcacca
      301 aagcggagtc aggtaatcga aagcgttgtc tcccgatgca tcgggattgc cgcactccaa
      361 aacgcctgtg aattgctcaa cgcttgctgg tctaagttac aatctccgca tgccaaacta
      421 tctcgatgtc tccacttggg cgcgacgcga cctgtttgag ttttttcgca cctacgagaa
      481 cccctacttc aacatctgtg gtcagctcga tgtcagcagt cttctcgatt tattgcagtc
      541 taaacctgac gtcagcccta ctctgaccta ccactattta gccttgcggg tggccaacga
      601 aattgagccg tttaagtatc gtctcagaga cgatcagata ttgattcacg acgtaattca
      661 cggaggaacg accgttcttc tccccaacga aagctttacg tttgcttact tcgactacca
      721 aattgcgttt gacaagttca ttaaggacgc acaggcttca gtggcggcag taaaacgcgg
      781 tgacactcct ttcaaaccgt tggacggaga tgaccgaatt cacttcactg tcttgccctg
      841 ggtctccttc accagttttg cccacgctcg caataaaacg cgcgccatct cggtccccaa
      901 gatcgcgttc gggaagttca ttcgaaataa tgaacgaacg tacttgccaa cctcagttga
      961 agtgcatcac gcaatgatgg acggattgca tgtgggacga ttcttcaacc ggatggaaga
     1021 gatgttggca gaaccctccg cttacttgta aagctccgca aattttctct ggttgcgtta
     1081 ctgaattcgc taagcgagaa tggcctgacg ttcattcagc ccctgaggtg attaggattc
     1141 gtttctaata tgcgctggtc acaatatttt attcctacgc ttcgcgaaga gcctgcggat
     1201 gcggaggtcg tttcacataa gctcttgcta cgcgctggtc tggtacgaca gctcggtgcg
     1261 ggcctctatt ccattctgcc tctcggacaa cgcgtcgccc tcaagattat cgagatcctg
     1321 cgcgaggaaa tgaatggcat cggcgcgcag gagttttatt tgccggcgct gaatccacgt
     1381 gagatttggg atgaatccgg ccgctgggac gtcatgggcg ataacatgtt tcgcctgaaa
     1441 gaccgcaaag gcgccgatct ttgcctgggc atgacacatg aggaggtttt cacttcgatt
     1501 gcgcgcagcg atctgaattc ctacaaacaa ctgcctcagg tttggtatca aatccaggtc
     1561 aagtttcgcg acgaagtgcg ccctaaatcg ggcgtgctgc gcgtgcggca gttcatcatg
     1621 aaagatgcct actctttcga cgcagacatg aacggtctcg acaagtcgtt caatgatcaa
     1681 cgcgcggctt accagcgcat cttcgatcgc tgcggtctgc aatatgtgat cgtcgaagcc
     1741 tcgtccggat caatgggagg atcggcatcg aatgagttca tggttaaaac ggatgccggc
     1801 gaagatatga ttgccacttg cgcgagctgc ggttacgcgg ccaatcttga gaaggctact
     1861 tcgcgtctcg atgaggttcg cgacggcgct ggactcgact ttccgtcaga atttcctact
     1921 ccgggcgtac gcacgattga cgatctggca aactttgccg gcggcgccgc cgccgagtcg
     1981 caaatcaaaa cactggttta tgttgattcc ggagcatcgg cttattacct ggcgctgctg
     2041 cgcggagatc atcaactcca cgaaacaaag ctattcgacg cgttgggtgc caacgctcgc
     2101 gctgccacgg gcgaagaaat ccaatcattg ctaggcgcca atcccggcag cctgggggcc
     2161 gtcggggcca aaaagaaggc ctctgaattg cagcctggcg caaccgttcg catagtagct
     2221 gacctcgcct tgcgcagccg gcgcaacatg accacggggg ccaatattga tgactatcat
     2281 ttgcgcggca ttgatgtagg ccgcgacatc aagattgatc aatgggccga tttgcgcacg
     2341 gttaattccg gagagaagtg tccgcagtgt gagaacggca ccctcgaagt tgccaaagcg
     2401 ttggagatcg gtcacatctt taaattaggc accaagtatt ccgagtcgat gggcgctaac
     2461 gttctcacgc aggaaggcaa gcaagtcccg attgtaatgg gaagctacgg aattggcgtc
     2521 gaacgaatca tggcctcggc gattgaactg catcatgacg acgacgggat catctggccc
     2581 aagtcgatcg cgccattcga cgtgatcgtc acaattacaa acatgaagca ggatgaggtg
     2641 cgcaacgccg gcgagagtct ctacaaggag ctgcaccgcg ccggtcttga cgtgttgctt
     2701 gatgaccgcg acgagcgggc gggagtcaaa ttcaaggatg ccgacttaat cggcattccc
     2761 tatcgcgtca ctgttggcaa aaaggtctcc gaaggattgg ttgagttgtt tgaccgccga
     2821 gcgaagacca ctgaagaagt gaagctggct gacataagcg gccgaatgca gaaacttgcg
     2881 cttgccggtt tgtagttttc taagacgtta gagatgaaat ccctctgcct ctcgttagtt
     2941 ctcctaatca ccagcgtcct gccctgcccg gctcaagagc tactgccgga tctggtgcgc
     3001 cgcataaaac cgtcggcggt ggcgattgag acgtttgacg cacgcggcga gaaaatcgca
     3061 cgtggcagcg gtttcttcat cgatttcgat cgtgtcgtca ccaatcgtca cgtaatcgag
     3121 gcggcctatc gcgccgaagt tcactcttac acggggatcg tgatgatcgt aaagggtgtt
     3181 ctcgccgtcg atgctgaagg cgatttggcg ctactcaagg ttgaagcacc tccgaatcaa
     3241 gtccgaccac tgccactcga tcgcacctct ccgcaggagg gcgaaagcgt cgtggtcatt
     3301 ggaaacccgt ttggtctgga aggaagcgtc acgaatggta tcgtgtcagc agtgcgcgac
     3361 attccgactt tcggcaggat cattcaaatc actgctccga tctcacctgg gtcaagcggc
     3421 agtccagtcg tgaacatgca gggacaagta atcggtgtcg caaccttgca aatcaccggt
     3481 ggacaaagcg tcaacttcgc aattccctcc gagcgtatct cgcaactgca aactgctacc
     3541 ttgatgacct tgccggaact ggtagcttcg tcgggccgta acaagcgcgc taaagccgtt
     3601 cagttttttc gcgacggctt gagttttctt tctcgtgacg actgtgagcg agcgcttcct
     3661 tatttcgaga aggcagttga ttccgatagc aactatgccg aggcctgggc ccagagcggc
     3721 ttttgcaaag agaagttggg ccggcatgca gaagcgattg aggcttcaaa gaaggctgtc
     3781 agcctgcgtc cggctgccga gtcgtacttc aacatcggct tggcaaacta ctatctgaaa
     3841 cagtatcgcg aatcagcaga ggcttatcgc gcggcgattc ggctggatcc ctacaattcc
     3901 gcagacgctt actacgctct cggactcgtc tatcgcgatt ggggaaaggc tgacgatgag
     3961 attcaagcgt ataaacaggc gatcaaactg cggcctgatt acacaagtgc gtatgagagg
     4021 ctgggcagcc gttactttcg ttccaggaaa tactccgaag ctgccgagat ctataagcag
     4081 ctggcgtcgt tgaaaccggg cgatgcgagc gcgaacaaca gtcttggcga aacttatctc
     4141 gagatgagca agttcaatga ggcgctcgac aactttcgcc aggcgattcg cttgaagccc
     4201 gattacggac gcgcgtattt caacctggga aagtgcttcc tggcgatggg gaaccgcgac
     4261 gctgctttgg agcagtacaa catcctgcaa aatctcgatc aggattgggc cgaaaagctc
     4321 gggaatctga tcaatccata aggtcagttg gtagctgtta gttgttagtg gaaaccaact
     4381 gacaatgaac tactgactat tgaccactat caactaacaa ctgactactg atcataatgg
//
LOCUS       DASUEX010000146         4370 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1516521, whole genome shotgun sequence.
ACCESSION   DASUEX010000146 DASUEX010000000
VERSION     DASUEX010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4370
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1516521"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="VFV61_02455"
     CDS             <1..509
                     /locus_tag="VFV61_02455"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HEX4899361.1"
                     /translation="PGHFLQFLHANRTSSKFGQVFIGYAFAEGWAHYSEEMMWDAGLG
                     NGDSETHIGQLISATLRNVRFLSAIGMHTGRMTVAESERMFREEGFQDAGTARQQAAR
                     GTFDPAYLNYTMGKLMIRKLRDDWTKTRGGKDAWQKFHDEFLKYGGPPIPLVRKAMMG
                     DDDSGSLF"
     gene            620..1177
                     /locus_tag="VFV61_02460"
     CDS             620..1177
                     /locus_tag="VFV61_02460"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX4899362.1"
                     /translation="MPAVARTLKPVPNPPGELENLFRAHHDRVFRTAHRITGSAADAE
                     DVLQTVFLRLIKGGESYDFANHPEAYLSRAAINASLDLLRSRNRSRAVGLEDVNVGGI
                     ESKGRSPEAQHADRELQLLIRQAVARLGETAGQMFVLRYYEGYDNQEIAKLLGTSQMV
                     VGVVLHRARTKLRKEIGHYLEKHHE"
     gene            1170..3866
                     /locus_tag="VFV61_02465"
     CDS             1170..3866
                     /locus_tag="VFV61_02465"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HEX4899363.1"
                     /translation="MNKTTESILDEVTAGIRNEQVDPATVKQAADRGWARLTTENLEL
                     KSTMTNTETEHIENCADFQTLIPAYLNNELSEARSLLLVDHTHECIPCRRAMKEARTR
                     TAAPQKTAVTPRRYSLQPVILRWGIAAALVIGFGLLALPFIQRYAPFGGELEATVQAA
                     EGQVYQIADTTSTPIIAGEKLAKGERVRTAKGAHAMLRLGDGTTIEMRDRSELSVTKN
                     AQGTTIHLHRGDIMVEAAKQKSGRLFVDTGDSLVSVTGTIFSVASGTKGSRVSVVAGE
                     VNLEHKGNERVVRAGEQVTTNPSIETIPVKDDVAWSRNAANYVQTLAALSSLSKELNK
                     VQQPGVRNSTHLLDLMPENTVVYAALPNLTATIAESHRIMQERINQNPALRQWYEKEH
                     SGHRGPGMDQVIDTVREFGEYLGDEVAVSLSMNDKGEPDSPLVLAELKNSAGFRQLLE
                     QQIAKYGGNKQGKPNLQFVDDPLTATAAAPEAGKKNDRLYVWINNNLFAASPQLTQLQ
                     ALAGVTQKGGGSPFTGTAFHNRIAQVYKEGAGLVVAANLEQLIAKNKAELAKGGDGAK
                     REDALQQLGILNLKYFALDQKETQGKTHTRAVLSFNEARGIPSWLAEPGPMGALEYIS
                     PDANVVAGFVVKNPVSLVDDLLGVVETVSPDLRKNLEKLQAERGFDIRRDFAAPLGGE
                     FAFAIDGPILPTPSWKMVFEVNDPQHLQQTLERVVTEVNKEAAKFGKTGLVWDRSEIS
                     GRTYYTLRSTDFGVEVNLTYVNGYMVVGPSRALVELAVRSQGSGYSLLRSARFTAGLP
                     ADGNANFSAVFYHNLGPLVQPFADRIASSANKLPQEQAEAIKKMAADMPPTLAYAYAQ
                     GDSITFAANTEGGPFGLSPASLLGMPNALEIQHILSKGMGKN"
     gene            4015..>4370
                     /locus_tag="VFV61_02470"
     CDS             4015..>4370
                     /locus_tag="VFV61_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899364.1"
                     /translation="MKLVSISLGSWLLGLSAILLTFCVVEGANLSSADLKGATVMSLV
                     VAALLFSLAYAPSLFWLRRRLGGCRPAALFPLASALVLNLPVFLIGILAIGRTLAPSE
                     ALAFIGAFILMGAAFG"
BASE COUNT          972 a         1182 c         1261 g          955 t
ORIGIN      
        1 ggccgggaca ctttctacaa ttcctgcatg ctaatcgcac ctcatcaaag tttggacagg
       61 tctttattgg ctatgctttc gccgagggct gggctcacta ctcggaagag atgatgtggg
      121 acgccggtct ggggaatggg gattcggaga cgcacattgg acaactgatc agtgcaacgc
      181 tccgcaacgt gagatttctt tctgcgattg gtatgcacac cggtcgaatg acggtagccg
      241 agtcggagcg gatgtttcgg gaggaaggtt ttcaggatgc gggaacggct cgacaacaag
      301 ctgcccgcgg cacctttgat ccagcttacc tgaattatac gatgggcaaa ctgatgatca
      361 ggaaactgcg agacgactgg actaagaccc gtggcgggaa agacgcttgg caaaaatttc
      421 atgacgagtt cttgaaatat ggtggcccgc caatcccgct cgtgcgcaaa gcgatgatgg
      481 gcgatgacga ttcgggatcg ctgttttagg cgctgaaaat agtctttctt cccattttta
      541 gcgaaaaaag ttcccctcgt ctgttaatag tccctcctgc tgctcgttta ctgagtggaa
      601 acaacttcag gagggcatcg tgccagcagt cgctagaacc ttgaaaccag tgccaaaccc
      661 tccgggagag ctggagaatc tcttccgagc acaccacgat cgggtctttc gcaccgctca
      721 tcgcatcact ggcagcgccg ccgatgccga agatgtgctg cagacagttt tccttcgcct
      781 catcaaaggc ggagaaagct acgacttcgc caaccatcct gaagcttatc tctcccgcgc
      841 ggccatcaat gcttccctcg accttctgcg tagccgcaac cgttcgcggg cggtcgggct
      901 ggaggatgtg aatgtcggag ggatcgaaag caagggcaga agcccagaag cgcaacacgc
      961 tgaccgcgag ttgcagcttt tgattcggca ggcagtggct cgtttgggtg agaccgccgg
     1021 gcagatgttt gtgttgcgtt actacgaagg ctacgacaac caggagatcg cgaagctttt
     1081 gggaacttcg cagatggttg tcggtgtggt gttgcaccgc gcgcgcacga aactacgaaa
     1141 agaaattggg cactatctgg agaaacatca tgaataagac cacggaatcc atcctcgacg
     1201 aggtaaccgc cggaatccgg aacgagcaag ttgatccggc cacggtaaaa caggcagcgg
     1261 accgcggttg ggcgcggctg acaacagaaa atttagaact gaagagcacc atgacaaaca
     1321 ctgaaaccga acacatcgaa aactgcgctg attttcagac gctcattccc gcatatctca
     1381 acaatgagct ttccgaagcg cgctcgctgt tgttagtcga tcacacacac gaatgcattc
     1441 cctgccgtcg ggcaatgaag gaagcacgca cgcgcaccgc ggcgccgcag aaaactgcgg
     1501 ttaccccgcg ccgctacagt ttgcaacccg tcatcctgcg ttggggcatc gctgccgcgt
     1561 tagtgattgg ttttggcctc ctggcgttgc cgttcatcca gcgttatgcg ccattcggtg
     1621 gcgagttgga ggcgaccgtc caggctgccg aaggtcaggt atatcaaatc gccgacacca
     1681 cgagcactcc gatcattgcg ggagagaaac tcgcaaaggg cgagcgcgtg cgtactgcga
     1741 aaggcgcaca tgcaatgttg cgcctgggcg acggcaccac gatcgaaatg agagatcggt
     1801 ccgagctctc agttactaag aatgcccagg gcaccacgat tcacctgcat cgcggcgaca
     1861 tcatggtgga agcggcgaag cagaagagcg gccgtctgtt tgtggacacg ggcgactcgc
     1921 tcgtttctgt caccggcacc attttttcag ttgccagcgg caccaaaggc tcgcgcgtat
     1981 cggtcgttgc cggcgaagtg aatctcgagc acaagggaaa cgagcgcgtc gttcgcgctg
     2041 gtgaacaggt cacaaccaat ccgagcatcg aaacgattcc tgtcaaggac gacgtggctt
     2101 ggagccgcaa cgctgcaaac tacgtgcaga ctctcgcagc gctctcgagt ctgagtaagg
     2161 aattgaataa ggtgcagcag ccgggcgtgc gtaactcaac gcaccttttg gatctgatgc
     2221 ccgagaacac cgtcgtctat gcggcgttgc cgaatttgac ggcgacgatt gccgagtcgc
     2281 atcgcatcat gcaggagcgc atcaaccaga accctgctct gcggcaatgg tatgagaaag
     2341 aacattcagg ccatcgcggt ccgggtatgg atcaggtgat cgacacggtg cgtgagtttg
     2401 gcgagtatct gggcgatgaa gttgccgttt cgctctcaat gaatgacaag ggtgagccgg
     2461 attcaccgct ggtgctggcg gagttgaaga actcggctgg tttccgccag ctcctggaac
     2521 agcagattgc caagtacggc ggcaacaaac agggaaaacc gaacctgcaa tttgtcgacg
     2581 atccgctgac tgcgaccgcc gcggcgccgg aagccggcaa gaagaacgat cggctttacg
     2641 tctggattaa caacaacctg ttcgccgcca gtccgcagct cactcaattg caagctttgg
     2701 cgggggtgac gcaaaagggc ggtggcagtc cgtttacggg aactgccttc cacaaccgga
     2761 tcgcccaggt ttacaaagag ggcgccggct tagtcgtggc agcgaatctc gaacagctga
     2821 ttgcgaagaa caaagcggag ctggccaagg gtggcgacgg cgccaagcgc gaagatgcgt
     2881 tgcagcagct cggcatcctc aatctcaaat actttgccct tgatcagaaa gagactcagg
     2941 gcaagaccca cacgcgtgca gtgctctcgt tcaatgaagc gcgcggtatt ccttcgtggc
     3001 tggcagagcc gggaccaatg ggtgcacttg aatacatttc gccggacgcg aatgtggttg
     3061 ccggcttcgt cgtgaagaat ccggtctcgc tcgtagacga tttgctgggt gtggtggaaa
     3121 cggtttcgcc tgacctacgc aaaaacctcg agaagctgca agcggaaagg ggttttgata
     3181 ttcgtcgcga ctttgccgca ccgctcggtg gtgaattcgc ttttgctatc gatggtccga
     3241 ttctgcccac accttcctgg aagatggtgt ttgaagtgaa cgatccacaa cacctccagc
     3301 agacgcttga gcgcgttgtg actgaggtta ataaggaagc ggcgaagttt ggcaagaccg
     3361 gactggtctg ggatcgttcc gagatttccg gacgcactta ttacaccttg cgttcgactg
     3421 acttcggcgt ggaagtgaat ctgacctatg tcaacggtta catggttgtt ggtcccagcc
     3481 gcgccctggt tgagcttgcc gtgagatcgc agggatcggg ttattcgttg ctgcgttctg
     3541 cccggttcac tgctggattg ccggctgacg gcaatgcgaa cttctcggcg gtcttctatc
     3601 acaatcttgg tccgctcgtg cagccgtttg cggatcgcat tgcgagttcg gccaacaagc
     3661 tgccgcaaga acaagcggag gcaatcaaga agatggccgc cgacatgccg ccgactctgg
     3721 cctacgcata tgcccagggc gatagcatta ctttcgcggc caataccgaa ggcggcccct
     3781 ttgggttgag tccggcgagc ttgttgggaa tgcccaacgc gctcgagatt cagcacatct
     3841 tgtcgaaagg catgggcaag aactgattca attgccgatt gccgatttgc gattgccgat
     3901 ttctggaatg atgaacgatg aacgcggaat gatgaatttc agaatcggca attggcaatc
     3961 gggaatcggc aatcggcaac attcatcgtt catcattcag aattcatctt tccgatgaaa
     4021 ctcgtatcaa tctctctcgg ctcctggctg ctaggcttgt cagcaattct gctgactttc
     4081 tgtgttgtcg aaggtgccaa tctcagctcc gctgatctca aaggcgcgac cgtaatgtct
     4141 ctggtcgtcg cggcgctgct tttctccctc gcatacgcgc cctctctctt ctggctacgg
     4201 cgccgccttg gtggctgccg ccccgctgca ctctttccgc tcgcctcagc gctcgtactc
     4261 aatcttcctg tctttctgat cggaatcctg gcaataggca gaactttagc gccatcggaa
     4321 gcgttagcat tcatcggcgc cttcatcctg atgggtgctg cttttggatt
//
LOCUS       DASUEX010000147         2760 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_27854, whole genome shotgun sequence.
ACCESSION   DASUEX010000147 DASUEX010000000
VERSION     DASUEX010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2760
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27854"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..1471)
                     /locus_tag="VFV61_02475"
     CDS             complement(92..1471)
                     /locus_tag="VFV61_02475"
                     /inference="COORDINATES: protein motif:HMM:NF013715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEX4899365.1"
                     /translation="MKNSAQPQGLEALDAVDAAGLPASSRPIKPGLRINVRGRSLNLR
                     GMRRSPTGLLRWLAILGPGLIATSAGNDAGGIATYSQAGAKFGYQLIWVMVILTVSFG
                     VVQEMCARLGAATGRGLLDLIRERFGIAWALFAVAVILIANGGVTVSEFVGIGAAMEL
                     LGVSKYVAVPLGAALIWYLVIFGSYAKVEKILLLMTLVFFAYPVAAFMAKPNWGEVAR
                     GAFIPTFNSDPAYLFLVVGLLGTTVTPYIQIFQQSSLVEKGVARRHYGPERIDAYFGA
                     VFSNLMSVSMIIATAATLYVVGQREIGSAADAARALEPVVGSAARVLFGIGLLGATLL
                     AGAVLPLATAYAVSEAFGMPKGVNLDFRRGRVFFSLFTALIVLGVVLALIPGIPVMKL
                     LVGVQVLNGLLLPIILVFILLLINDKNLTRELKNTRINNALGIGTLILITTAVLVMIG
                     GQVLEWLGR"
     gene            complement(1468..>2760)
                     /locus_tag="VFV61_02480"
     CDS             complement(1468..>2760)
                     /locus_tag="VFV61_02480"
                     /inference="COORDINATES: protein motif:HMM:NF015413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX4899366.1"
                     /translation="LFPGWLGKGGPEIMVMLSQLLRFKIQDEHGGQAGLSDLSVALLE
                     GDHPRVTCLYFSDEMRQKCSLPWEKVRSIDWQERVIKVADLAEAEEESKESMAKAVLL
                     SAGILDAMVLDLQNRRATRANDLLLEESQGNQLLLRAADTGLGGIARRLTRGLFGPVA
                     ESALYDWKYVEFLRGDPGAVRSEAGEHLRISRLPPGEIARLTEALPYLHAAELITLLA
                     DPKAADTLEKMTAERQLQVFEELDEEQALRLLELMAPDIATDLLARLHPQTMRRYVER
                     LPRKQAERILELLRYPEHTVGSIMTNDAVYVCGDLTVAAARDKLREPLKNPDFVYFVY
                     VVEDEESRRLRGVISIRDLITAPDEKKLEELMDPYLVTLQPLEAADESAHRVLDSHLA
                     ALPVVGEDKRLLGVVTVDAAVAQVAPRSWSAQAPRIFS"
BASE COUNT          654 a          845 c          716 g          545 t
ORIGIN      
        1 cgttatttca attcccgcct gcggcgggaa gcgggggcaa gcccaccttc cggactgagc
       61 atttgacccg acccttcggt tgcctttgct atcagcgacc cagccactcg agcacctgcc
      121 cgccgatcat caccagtacc gcagtcgtaa tcaggatcag tgtgccgatg cccaacgcgt
      181 tgttaatccg cgtattcttc agttctcgcg tcaggttctt gtcattaata agcagcagga
      241 tgaagacgag aatgatcggc aacagcagac cgttcagcac ttgaactccc accagcaact
      301 tcatcaccgg gatgcccggg atcaaagcca gaaccacacc caacacaatt aacgccgtaa
      361 agagactaaa gaaaacgcga ccgcgccgga aatcaagatt gacgccctta ggcatgccga
      421 acgcttcgct gaccgcatag gctgtcgcga gtggcaagac tgcgccggcg agaagtgtgg
      481 cgcccagcaa accaatcccg aaaagcacac gagctgcgct tcccaccacc ggttccagtg
      541 cgcgcgcggc gtccgcagcc gagccgattt cacgctgacc gacgacatac aaggtcgcag
      601 cagtggcgat gatcattgac accgacatca aatttgaaaa gacggcgccg aaataggcat
      661 caatgcgctc cggtccgtag tgacgccgtg ccactccctt ttccaccagg gagctctgtt
      721 gaaagatttg aatgtaagga gtgacagtcg taccgagcaa accaactacc agaaaaaggt
      781 acgccggatc agagttgaaa gtaggaataa atgcgccgcg tgccacctcg ccccagtttg
      841 gtttggccat aaaggctgcg accggatagg caaagaaaac caatgtcatt aacagcagaa
      901 tcttttctac tttcgcgtag gagccgaaga tcaccagata ccagatcaag gccgcgccca
      961 ggggaaccgc cacatactta ctgaccccca gcagttccat ggcggcgcca atgcccacaa
     1021 attcgcttac cgtcacgccg ccattcgcga tcaaaataac cgccactgcg aacaaggccc
     1081 aggcgatccc gaatctttcg cgaatcaggt caagcaagcc cctgccagtt gccgctccca
     1141 gccgggcaca catttcctgc accacgccaa acgaaacagt caggatcacc attacccaga
     1201 tcaactggta accaaacttc gcgcctgctt gactgtaagt ggcaatgccg ccggcgtcgt
     1261 taccggcaga agtggcaatc aatcccggcc caaggatggc gagccagcgc agcaagcctg
     1321 tgggactccg ccgcatgccc cgcaggttga ggctgcgacc gcgaacatta attcgcagtc
     1381 ccggcttaat gggacgcgaa ctcgccggca gccctgcggc atctactgcg tcgagcgcct
     1441 cgagtccctg cggctgcgct gaattcttca tgagaaaatc ctgggagctt gtgcgctcca
     1501 actacgcggc gctacctgcg ccactgcggc atcaactgtg acgacaccga gcagtcgttt
     1561 gtcttcccca actaccggca acgcagcgag gtggctatcg agaacgcgat gggcactttc
     1621 atccgcagct tccagtggct gaagcgtgac caggtaggga tccattagct cttccagttt
     1681 cttttcatca ggcgccgtaa tcagatctcg aattgaaatc acgccgcgca gacggcgcga
     1741 ttcttcatct tcgacaacgt aaacgaagta gacaaaatcc ggatttttca agggctcgcg
     1801 caacttgtca cgtgctgctg ctactgtgag atctccgcaa acgtagactg cgtcgttagt
     1861 catgatgctg ccaaccgtgt gttccggata gcgcaagagt tccagaatcc gttcggcttg
     1921 tttccgcggt aaacgttcga cgtaccggcg catggtttgc gggtgcaaac gagccagcag
     1981 atccgtagca atatcaggcg ccatcagctc gagtagacga agcgcctgct cctcatcgag
     2041 ttcctcaaaa acctgaagct gacgttcggc tgtcattttt tccagagtgt cggccgcttt
     2101 cggatcagcc aaaagggtga ttagttcggc ggcatgtaag taaggcaaag cctctgtgag
     2161 acgcgcgatc tcgccgggag gcaagcgact aatacgcaag tgctcgcccg cttcactcct
     2221 caccgccccg ggatcgcccc gtaagaactc cacgtacttc cagtcataaa gtgcgctttc
     2281 cgcaacaggg ccaaaaagcc ctcgcgtcaa ccggcgcgcg attccgccga gacctgtatc
     2341 ggcggctcgc aataaaagtt ggttgccctg cgattcttcc agcagcaaat cgttggctcg
     2401 cgtggcacga cggttttgca gatcgagaac catggcatcg aggatgccgg cactgagaag
     2461 taccgctttt gccatagact ctttcgattc ctcctcggct tcggcgagat cggcaacctt
     2521 gattacgcgc tcctgccaat cgatcgagcg cactttctcc caaggtaggg aacatttctg
     2581 acgcatttcg tcgctgaagt agaggcaggt cacgcgcgga tggtcacctt cgaggagtgc
     2641 gacgctcaag tcgctcaatc ctgcttgccc gccatgctca tcctggattt tgaagcgcag
     2701 cagttgcgag agcatcacca tgatctcggg acctcctttg ccaagccagc caggaaaaag
//
LOCUS       DASUEX010000148         3404 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1395797, whole genome shotgun sequence.
ACCESSION   DASUEX010000148 DASUEX010000000
VERSION     DASUEX010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3404
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1395797"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..603)
                     /locus_tag="VFV61_02485"
     CDS             complement(<1..603)
                     /locus_tag="VFV61_02485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEX4899367.1"
                     /translation="MKHAITNLSRTFLAVLLFATFGPVNAQQETFTRTDAMISMRDGV
                     RLNTHVYTPVSTTEKFPILLLRTPYGIGNLSSAQLAAALPELAADGYIVVQQDIRGRF
                     KSEGQFVMLRQPRDPKDKTAIDESTDTYDTIEWLLKNVSNNNGRAGIAGTSYGAWLAV
                     MAMLDPHPALKAVVQQASPADMWIGDDFHHNGAFRLSYGFE"
     gene            complement(657..2351)
                     /locus_tag="VFV61_02490"
     CDS             complement(657..2351)
                     /locus_tag="VFV61_02490"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HEX4899368.1"
                     /translation="MKANLHKLLSLTLILALSATALAQGRNPASFDVIIKGGTVYDGS
                     GRPPTRADVGLRGDRIAAIGNLSRATATTVVDAKGLAVAPGFINMLSHSESSLIVDPR
                     SLSEIKQGVTTQIFGEFSMGPLNDQMKQRLSANRGDSRFQIEWTTLAEYLTYLEKRGI
                     PQNVASFIGAATIREHVIGLEDKQPTPAQLEEMRELVRREMEAGALGITTALIYPPAF
                     FAKTEELIEMCKVAAKYKGKYTTHMRSEGNQLIEGVQETIRISREAGLPAEIYHLKAS
                     GAANWPKMDQVFKMIQNARRRGLKITANMYTYPAGGTGLDASMPPWVWDGGREAGYKR
                     LQDSDTRKKIADAIRTPSNDWENLYLLAGSPDRLLLASFRSEQLKPLVGKTLGEVAKM
                     RGKDPVETIMDLVLEDRSRIGTIYFLMSEDNLKKQIRQPWVSFGSDAASIAPEGNVLR
                     SSAHPRAYGNFARLLGKYVREEKVISLSEAVRRLSSLPATNLGLDHRGFLRPGMFADV
                     VVFDPQTIADRATFENPHQLSVGVQHVFVNGTQVLKDGEHTGAKPGRALWGPGKVK"
     gene            complement(2385..>3404)
                     /locus_tag="VFV61_02495"
     CDS             complement(2385..>3404)
                     /locus_tag="VFV61_02495"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEX4899369.1"
                     /translation="YLTGAGDVPGEDPVSVGLLGADFAIEDGRYRIKHIFTGENWNPE
                     LRAPLSAPGIQVAEGDYLLEVNGRALAPPTNLYSLFEGTAGRQTLIRVNKTASMEGSR
                     VVTVIPVPSEDGLRARAWIEDNRRLVDKLSGGRLAYVWLPNTAGPGYTAFTRYYYAQQ
                     NKEGAIIDERYNQGGQVADYIVNELDRKLMGFFAQRDGEPANSPTAGIYGPKVMIINE
                     SAGSGGDALPYMFRQRKLGPLVGTRTWGGLVGTLGFPPTIDGGGITAPNLAFYDLSGK
                     WAVENEGVPPDIEVEYTPAEVNKGHDPQLERAVQEAMRLLEQNPVRRVPRPAPIDRVS
                     KPGTR"
BASE COUNT          681 a          987 c          928 g          808 t
ORIGIN      
        1 ttcgaaacca taactcaacc ggaaagcgcc attgtggtga aagtcgtcgc ctatccacat
       61 gtccgccggc gacgcctgct gaacaactgc cttgagggcc ggatgcggat caagcatggc
      121 catcaccgcg agccacgcgc catagctcgt tccagcgatc ccagctcttc cgttattgtt
      181 ggacacattt ttcaggagcc attcaatcgt gtcgtaagtg tcagtgcttt cgtcgatggc
      241 ggttttatcc tttggatccc gcggttgccg aagcatcacg aactgacctt ccgacttgaa
      301 ccgtccgcga atgtcctgct gcacgacgat gtatccgtcg gccgccagct cgggaagcgc
      361 agcggccagt tgcgccgagc ttagattgcc gatcccgtat ggagtcctga gcagaagaat
      421 cgggaacttc tcggtggtgc taaccggagt gtacacatgt gtgtttagcc gcactccatc
      481 ccgcatcgag atcatcgcgt cagtgcgagt aaaagtttcc tgctgcgcgt tgacagggcc
      541 gaaggttgcg aagagcagca ctgcgaggaa cgtgcgactt agatttgtga tggcgtgttt
      601 catttcttgt cgttcccgat agttgtcctt tgtcctgagt ccgttgaaat gctcaattac
      661 ttcaccttgc ccggtcccca aagcgccctt ccaggttttg ctcccgtgtg ttcgccgtcc
      721 ttgagtacct gcgtgccgtt cacaaatacg tgttgcacgc cgaccgacag ctggtgcggg
      781 ttttcaaacg tggcgcggtc agcaatcgtt tgcggatcaa acaccacgac atctgcgaac
      841 attccgggtc taagaaagcc gcgatgatcc agcccgagat tagtggccgg taagcttgag
      901 agccgccgca ccgcttcgct cagcgagatc accttttctt cgcgcacata tttgcccagc
      961 agccgcgcaa aattgccata ggctcgtgga tgcgccgaag acctcagaac gttaccttcc
     1021 ggtgcaatcg aagctgcatc agagccgaac gaaacccagg gttggcgaat ctgcttttta
     1081 aggttgtctt cactcatcag gaagtaaatt gtgccgatgc gcgatcgatc ttcaagcacg
     1141 agatccataa tggtctcgac gggatctttg ccgcgcatct tcgcaacttc acccagagtc
     1201 ttaccaacca acggcttcaa ctgctcgcta cggaaactgg ccagcaggag tcgatcgggc
     1261 gaacctgcga gcaggtagag attctcccag tcgttcgagg gagtgcgaat ggcgtccgca
     1321 atcttcttgc gcgtatcaga atcctgaagg cgcttgtagc cggcttcgcg cccgccatcc
     1381 caaacccatg gcggcatact ggcgtccagg ccagtgccgc cggccggata ggtgtacatg
     1441 ttggcagtga tctttagccc ccggcgtcgc gcgttctgga tcatcttgaa cacctgatcc
     1501 atcttgggcc agttggccgc gcccgatgcc ttgaggtgat agatctctgc cgggagtccg
     1561 gcctcgcgac tgatgcgaat ggtttcctgt acgccttcga tcaactgatt gccttcgctg
     1621 cgcatgtgcg tggtgtactt gcctttgtat ttggcagcga ccttgcacat ttcgatcaat
     1681 tcttcggtct tggcgaaaaa tgccggggga taaataaggg cggtcgtgat gcccagcgcc
     1741 ccggcctcca tctcacgtcg caccaattcg cgcatctctt cgagttgcgc aggtgtcggt
     1801 tgcttgtctt ccaaaccaat cacatgctca cgaatggttg ccgctccaat gaacgaggcg
     1861 acgttttgag ggattccgcg tttctcgaga taggtgagat attcagccag cgtcgtccac
     1921 tcgatttgaa atctggagtc accccggttt gcgctcaggc gctgcttcat ttgatcgttg
     1981 agcgggccca tggaaaattc gccgaatatc tgggtagtga cgccctgttt gatctcgctc
     2041 agggatcggg gatctacgat caaagacgat tcagagtgcg agagcatgtt gataaagccc
     2101 ggcgctacgg cgagaccttt agcgtccaca acggttgttg ctgtggctct actcaagttt
     2161 ccgatggcag cgatgcggtc gcctctgagc cctacatcgg cccgcgtcgg tgggcgacca
     2221 ctgccgtcat agacagtgcc gcccttaata atgacgtcga atgaagcggg attgcgccct
     2281 tgcgcaagcg ccgtggctga tagtgcaagt atcagcgtta gagatagaag cttatggaga
     2341 ttggctttca tgattatgtt gcgtttcagt aggtctgaac aaaactatct agttcccggt
     2401 ttcgacaccc tgtcgatggg cgcgggacgc ggcactcgcc gcaccggatt ctgttcaagc
     2461 agcctcatcg cctcctgcac cgctcgctcg agctggggat cgtgcccctt gtttacttcg
     2521 gctggcgtgt actccacctc gatgtccgga ggaacgccct cgttttccac ggcccacttt
     2581 cccgacaggt catagaacgc caaattaggt gcggtgattc caccgccatc gatcgtcggc
     2641 ggaaagccga gcgtgccaac gagcccgccc caggtgcggg taccaaccag cggtccgagc
     2701 tttctttggc gaaacatgta cggcaacgcg tccccacccg agcccgccga ctcgttaatg
     2761 atcatcacct taggtccgta gattcccgca gttggcgaat tcgcgggctc gccgtcacgc
     2821 tgggcgaaga aacccattag ttttctatcc agttcattga cgatgtaatc agctacctgt
     2881 ccgccctggt tataacgttc atcgatgatc gcgccttctt tattctgttg cgcatagtag
     2941 taccgagtga aagctgtgta gcctgggcca gccgtgttgg gaagccaaac ataggccaag
     3001 cgtccacccg aaagtttgtc aaccaatcga cgattgtctt cgatccaggc tcgtgcgcgg
     3061 aggccatctt cacttggaac agggatcacg gtgacaacac gtgaaccttc catcgaggca
     3121 gtcttgttga ctcggatcag cgtctgcctt ccggctgtgc cctcaaacaa gctgtagaga
     3181 tttgtgggcg gtgccagcgc gcggccattt acttcgagca ggtaatctcc ttcggctacc
     3241 tggatccccg gcgcgctgag cggcgcgcgc aattcaggat tccaattctc accggtaaag
     3301 atgtgtttga ttcgataacg tccgtcctcg atcgcaaagt ctgctcctaa caacccaact
     3361 gatactggat cctcaccggg gacgtcgcct gcgccggtca gata
//
LOCUS       DASUEX010000149         2209 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_358680, whole genome shotgun sequence.
ACCESSION   DASUEX010000149 DASUEX010000000
VERSION     DASUEX010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358680"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..791)
                     /locus_tag="VFV61_02500"
     CDS             complement(<1..791)
                     /locus_tag="VFV61_02500"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX4899370.1"
                     /translation="MNRKLIRDSFQPQGLNPSISHTAMTQNRRQFLYNAAVFAVSARL
                     SFGVQQRKESMRIILLGTKGGPRVGETGRKNPATLLLINDKPYLIDCGYGVSRQMLAA
                     GVALNRLRYIFITHHHSDHNLEYGPVIYNAWATGFPIKVDAYGPPGLQKMTEDFFSYM
                     KFDIDTRIVDEGRPDLRQFVTAHDFNKAGIVLENEDVKVSAVVVRHPPIKYSFAYRFD
                     GRDRSVVISGDTAYAPELAEFARGADVLVHEVMYMPGVEALLKRVP"
     gene            complement(788..1666)
                     /locus_tag="VFV61_02505"
     CDS             complement(788..1666)
                     /locus_tag="VFV61_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899371.1"
                     /translation="MTTTHKIGRFFQHIGYQIHRGTLPLRRSGFSTFDEERILNHYIT
                     EFFGAGHRGTAVDIGAGDGRTGSNTLALFKNGWKGIGIEGDDRKAAKLATLYRQFPNV
                     EARRFRVTPDNVLALLEGHGVETNFSLLSIDIDSYDYLVLDAILSRYRPGIVISEINE
                     KIPPPIKFSVNYDPDFQLREHFYGYSIAYLEELCGKHDYAVIDLEYNNVFLAPREIAG
                     GRALTVEEAYRRGYADRPDRHKKFHRNENMEFLQSASPEEGIRFIRQFFAQFNGKYEL
                     SVASGGGEGASMNRES"
     gene            complement(1833..>2209)
                     /locus_tag="VFV61_02510"
     CDS             complement(1833..>2209)
                     /locus_tag="VFV61_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899372.1"
                     /translation="ATATEGDPVNFKVKDDLKINGKVVIAAGTLVKGTIANAQKAGRM
                     GKGGKLGIRVESTTAVDGQKVRLRASKGGEGGDKVGTVIALSVFFGLFGLLKKGNNAK
                     IKKGTEIKVYTDEVKSVKAGAQ"
BASE COUNT          504 a          608 c          553 g          544 t
ORIGIN      
        1 ggcacccgct tcaacaatgc ctcgactccc ggcatgtaca tcacttcatg aaccagcaca
       61 tcggcgcccc gggcaaattc ggcaagctcg ggtgcgtagg cagtatcccc ggagatcact
      121 accgatcgat ctcgtccatc gaaacgatat gcgaaagagt acttgatagg tggatggcgc
      181 accaccactg cgcttacctt gacatcttcg ttctccagca caatgccggc tttgttgaaa
      241 tcatgtgcag taacaaactg ccgcagatcg ggccttccct cgtcaacgat ccgcgtgtcg
      301 atatcaaact tcatgtagct gaaaaagtct tcagtcattt tttgcaagcc gggtgggcca
      361 taggcatcca ccttgatcgg aaacccggtg gcccaggcgt tatagatgac cgggccatat
      421 tccagattgt gatcggaatg atgatgggtg atgaagatgt aacgaagccg gttcagtgcg
      481 acgccggcgg ccagcatttg cctcgagact ccataaccgc aatctatgag gtaaggttta
      541 tcgttgatca gcagcagcgt cgccggattc ttacggcctg tttcgccaac gcgagggcca
      601 cctttcgtgc caagcaggat gatgcgcatt gattccttcc tttgctgaac gccaaacgag
      661 aggcgggcag agactgcaaa aactgctgcg ttatacagga actgtctgcg attttgcgtc
      721 atggccgtgt gagagatcga cgggtttaac ccctgcggtt ggaacgaatc acgaatcaat
      781 ttacgattca cgattcacga ttcatgctgg cgccttctcc cccacccgaa gcaacactca
      841 attcatactt gccgttgaac tgcgcgaaga attgccgaat gaatcttatg ccctcctcgg
      901 gtgaggcgga ttgcaggaat tccatatttt cattccggtg aaactttttg tgccggtctg
      961 gtcggtcagc gtacccgcgc cgataggcct cttcgactgt caaagcgcgg ccacctgcta
     1021 tttcgcgcgg agcgagaaag acgttgttgt attcgagatc gatcaccgcg taatcgtgtt
     1081 ttccgcagag ctcctccagg taagcgatgc tgtaaccgta aaaatgttcc cgtaactgaa
     1141 agtctggatc atagttcacg ctgaacttaa tgggcggcgg tatcttctcg ttaatctcag
     1201 aaatgacaat gccaggtcga taacggctga ggatcgcgtc caagaccaga tagtcatagc
     1261 tgtcgatgtc aatactcagc agactgaaat ttgtctcgac cccatggcct tccagcaaag
     1321 cgagcacgtt atcaggagtc acgcgaaatc ggcgagcctc gacgttcggg aattgtcgat
     1381 agagcgttgc aagtttagcc gccttgcggt cgtcaccctc gatgcctatg cccttccaac
     1441 cattcttgaa cagcgccagc gtattggagc cagttctccc atcgcctgcg ccgatatcga
     1501 ccgccgttcc gcgatggccg gcgccaaaga attcagtgat gtaatgattg aggatgcgtt
     1561 cttcgtcaaa agtcgagaag ccactgcgcc ggagaggtaa ggtgccccgg tgaatttggt
     1621 agccgatgtg ctgaaagaac cgcccaatct tatgagtcgt tgtcattacc gcaatgtttt
     1681 ccgtggagtg aatctggaga ctgcaatgct gtctcgctac ttgatattga tgagaattct
     1741 aacaaccacc tgcgcgagat tgccagatgc tgagaagact aaggcgagct gtctgttgcg
     1801 cagctcgcct gacatcaagg cgtcgaagtc ccttactggg ctccggcttt taccgattta
     1861 acttcatccg tatagacctt tatttctgtg cccttcttta tcttggcgtt gttccccttc
     1921 ttaagcagac caaataaacc gaagaacacg gataaggcga tgaccgtacc gaccttatcc
     1981 ccgccttctc caccttttga ggctctgaga cgcacctttt gtccatcgac agcagtggtc
     2041 gattccacac ggattccgag tttaccgccc ttgcccatgc gaccggcttt ttgcgcgttg
     2101 gcaatcgtac ccttgaccag cgtacctgca gcaatgacaa ccttcccatt aatcttcaga
     2161 tcgtccttga ccttgaaatt cactggatca ccctcggtgg cggtggcac
//
LOCUS       DASUEX010000150         4052 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1396778, whole genome shotgun sequence.
ACCESSION   DASUEX010000150 DASUEX010000000
VERSION     DASUEX010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4052
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1396778"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(140..994)
                     /locus_tag="VFV61_02515"
     CDS             complement(140..994)
                     /locus_tag="VFV61_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899373.1"
                     /translation="MSQSPVESAITPPLEVGKPRRIRFDNRYVAPLFITFILLVGHLS
                     YGILESYDKTALAIAAAIVTELVLGRMFFGKWLNLASAYITGISVGILVRSPAYWPFL
                     LCSVISIMSKYVLRVKGRHIWNPSNFGIAVLLFLAPETMAILSIQWGNNLLPIVVIWI
                     LGSIIIWRARRFHICATYVVAFFLFAFLRSWITGHPWQAEVAPITGPMYQLFVFFMIT
                     DPKTTVRSRLAQCVVAFLVAFVEFLLRLNQVVYAPIYALFLVGPAANLIEIWLDSRHQ
                     KDAVTVTA"
     gene            complement(991..2784)
                     /locus_tag="VFV61_02520"
     CDS             complement(991..2784)
                     /locus_tag="VFV61_02520"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HEX4899374.1"
                     /translation="MRGRPAKLLLALLFVALLATPLLLKRLAARREAGKSKLEASQAL
                     SRYGFHFQEVAQASGISFVHQAPKLDPKLDHIMPQVASMGAGASVVDFDRDGWQDIYL
                     TNSGEGSKNCLYRNLGNGGFKDVAGELGIADVNQLGTGVSMGAVWGDYDNDGYEDLFL
                     YKWGRPELFHNDGGRSFTRATDQAGLPAWVNANTAIWFDYDSDGLLDLFVGGYYSEAI
                     NLWNLQTTKMMPESFEYAKNGGRKYLFHNLGSGKFEEVSGKLGINSRRWALAAAAGDL
                     RGSGHQDLFIANDYGVSELYFNDGKRFRDVGEQTGVGFAPKSGMTAAFGDVLNQGTYA
                     IYVSNISEDGVLIQGNNLWVPQEGTSGDGLRYENLARALGVEMGGWSWGMQFGDLNND
                     GTLDVYLTNGYVSADRNKSYWYDFSKVAGGNSSIISDAANWPAMEGRSLSGYQHKRVW
                     LNDGAGKFNDVAQAVGATDTYDGRSVALADLWNRGVLDVIVTNQRGPLLIYKNTVTPE
                     NKWIAFELEGTASNRSALGAEVKLFWSGRQQAQTVSGGSGFCAQNQRILHFGLGKNAQ
                     IEKAVIRWPSGKTQAIDNPAMGRVNKIKEAA"
     gene            complement(2781..>4052)
                     /locus_tag="VFV61_02525"
     CDS             complement(2781..>4052)
                     /locus_tag="VFV61_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899375.1"
                     /translation="EMKFVKDAVPWGSAIGLRINTIGTARVEATEEWYKIGGDELLPR
                     DGFYDLRITAELWETYYYDQLNLMTVDHPAGTDIFVDERFVIPPAKLAITTVEKPRKI
                     AHAVDDSGKDVTDIVSDLDDQYLDNFGRGRYQGVSRHHYVEVDLGEDAPQSGPLWLIA
                     KGWMHPTDSSINVAISQGQEVRAQPLSMEVPDGKGGWVVARPNLGFPAGRKKICLFDL
                     SGVFRPGTPRRLRLRTNLEIFWDSIEWAKGLPDTQLKTIKINPAVADLHYRGYSVIDK
                     PNDSSPEVPHYDRVTASKQIWRDLIGYYTRFGDVRVLLEKIDDRYVIMNAGDEISLRF
                     PEHQPPPPGWVRDYLLMGDGWIKDGDYNSTFSKTVLPLPYHAKNEYTTPPGKLEDEWV
                     YKKYPEDWQKYHTRYVTPAVFRNALRPRGDR"
BASE COUNT          961 a         1173 c         1023 g          895 t
ORIGIN      
        1 ccggctcttt acaggctgac agcagcagca accaaataag cagcgagcaa cagatgatcg
       61 ggtaacgtgg cattagtatc gggaatctcc ttgggtaaca gaacaactcg tcgctgcggc
      121 ttcggctttg caggcggtat cacgctgtaa ctgtcacggc gtccttctgg tggcgcgaat
      181 ccaaccagat ctcgattaga ttcgctgccg gccctaccag gaacagtgcg tagatgggag
      241 cgtagacaac ctgattgagc ctgagaagaa attcaacaaa cgcgacaagg aatgccacca
      301 cacattgggc cagacgcgag cgcacagtcg tctttggatc agtaatcatg aagaaaacaa
      361 agagttgata catggggccg gtgatgggcg ccacttctgc ctgccaggga tggccggtaa
      421 tccaactgcg caggaacgca aacaagaaga acgcgacaac atacgtggcg cagatatgaa
      481 agcggcgcgc ccgccaaatt atgatcgagc ccaaaatcca gatgacaaca atcggcaaga
      541 gattgttgcc ccattgaata ctcagaattg ccattgtctc tggcgccaga aacaacagca
      601 cagcgatgcc gaagtttgac ggattccaga tgtgacgccc tttcacacgc aacacatatt
      661 tcgacattat cgagatcacg ctgcacagca gaaaaggcca gtaggccggc gagcgcacca
      721 agattcctac gctgattccc gtgatgtaag cgctggcaag attgagccac ttcccaaaaa
      781 acatgcgacc gagaaccaac tcagtaacga ttgccgccgc gatcgccagc gccgttttgt
      841 catagctttc gaggatgccg taggaaagat gcccaaccaa taagatgaag gtgataaaaa
      901 gaggagcaac gtaacgattg tcgaaacgga tccgccgtgg tttccccact tccagtggcg
      961 gagtgattgc agactcgacg ggagactgac tcatgcagcc tccttgatct tgttcacccg
     1021 gcccattgct gggttatcaa tggcttgagt ttttccagaa ggccagcgaa tcactgcctt
     1081 ttcaatctgc gcatttttgc ccaaaccgaa gtgcagaatt cgttgattct gggcgcagaa
     1141 gccactgcca ccggaaacag tttgggcttg ctgtcgtccg ctccagaaca gtttaacttc
     1201 ggcgcccagc gcgctgcggt tactggccgt gccttccaac tcaaacgcga tccacttgtt
     1261 ctccggcgtg actgtgttct tgtagatcag caatggccca cgctggttgg taacaatcac
     1321 atcgagcaca ccccggttcc atagatcggc gagcgctacg gaacgtccat cgtaagtgtc
     1381 cgttgcacct accgcctggg ctacgtcgtt aaatttaccg gcgccatcgt tcaaccaaac
     1441 gcgcttatgc tggtaaccgg acaaactgcg cccttccatc gccggccaat tcgcggcatc
     1501 acttatgatc gagctattac cgccggcaac ttttgaaaag tcataccaat agcttttgtt
     1561 ccgatccgca gagacgtagc cgttagtcag gtagacgtct aacgtcccat cattgttgag
     1621 atcgccaaac tgcatacccc agctccaccc tcccatttcg acacccaatg cgcgagccag
     1681 attctcatat cgcaggccgt cgccagatgt gccttcctgg ggcacccaga ggttattccc
     1741 ctgaatgagc acaccatctt cggaaatgtt cgagacgtag atcgcatacg tgccctggtt
     1801 cagaacatcg ccaaacgcgg cggtcatgcc gcttttggga gcaaagccga caccggtctg
     1861 ttcgcccaca tcacggaatc gttttccgtc gttgaagtac agctctgaaa ccccatagtc
     1921 attcgcaatg aacagatcct gatggcccga acctctcagg tcgcccgcgg ctgccgccag
     1981 cgcccagcga cgactgttaa tgccgagttt gccgctaacc tcttcaaact ttcccgatcc
     2041 gagattatga aacagatact tccgtccgcc gtttttcgca tattcgaagc tctcaggcat
     2101 catcttcgtc gtctgcaaat tccagagatt gatagcctcc gaatagtatc caccgacaaa
     2161 tagatcgagc agtccatcgc tgtcatagtc gaaccagatc gctgtgttgg cgttcaccca
     2221 ggcaggcaat ccggcttgat ctgtcgcacg cgtaaaagag cggccgccgt cgttgtgaaa
     2281 aagttcgggg cgtccccatt tgtatagaaa aagatcttcg tagccgtcat tgtcgtagtc
     2341 tccccagacc gcgcccatcg aaacgcccgt gcctaattga ttgacatccg cgatgcctag
     2401 ttcgcccgcc acatccttga acccgccgtt gccgaggttc cgatacaaac aattcttgct
     2461 accttcaccg ctgttggtta aatagatgtc ctgccaaccg tcacgatcaa agtcgacgac
     2521 tgaagcgccc gctcccatcg aggcaacctg cggcatgatg tgatcgagtt taggatccaa
     2581 cttcggggct tgatggacaa agctgattcc agaggcctgt gccacttcct gaaaatgaaa
     2641 accgtagcgg ctgagcgcct ggctggcctc caatttggac ttcccggctt ctcgacgcgc
     2701 cgccagacgc ttaagcagca gcggcgtcgc cagcaacgcg acgaagagaa gggcaagcaa
     2761 aagttttgca ggacgacctc tcatcgatct cccctggggc ggagggcgtt tcggaaaacc
     2821 gctggcgtga cgtaacgcgt gtgatacttc tgccagtcct caggatattt tttgtaaacc
     2881 cactcatcct cgagtttacc cggtggtgtt gtgtactcgt tcttcgcatg atacggcagt
     2941 ggcagcactg tcttggaaaa agtagagttg tagtcgccgt ccttaatcca gccatcgccc
     3001 attaatagat agtcacgcac ccagccgggg ggtggtggct gatgttccgg gaaacggagt
     3061 gaaatctcat cgccggcatt catgatcacg tagcggtcgt caattttctc gagcagtacg
     3121 cgcacgtcgc caaagcgcgt gtaataaccg atcagatcgc gccagatctg tttcgaggcc
     3181 gtaacccgat catagtgcgg gacctcgggc gaagaatcat tcggcttatc gatcactgag
     3241 tacccgcggt aatgcaagtc cgccaccgcc ggattgatct tgatcgtttt caattgcgta
     3301 tcgggaaggc ccttcgccca ctcaatcgag tcccagaaga tctccagatt tgtgcgcagc
     3361 cgcaagcggc gcggtgtgcc cgggcggaac acaccgctga gatcgaacag gcagatcttc
     3421 ttgcgccctg caggaaagcc aaggtttggt cgggccacaa cccagccacc cttgccatcg
     3481 ggaacttcca tgctcaacgg ctgtgcgcga acttcctgac cctgactgat cgccacgttg
     3541 atcgaagaat cggtcggatg catccagcct ttagcgatca accacagggg accgctctgc
     3601 ggcgcgtctt cgccaagatc aacttcgacg taatggtggc gactaacacc ttggtagcga
     3661 ccacgcccaa agttatcgag gtattggtcg tcaaggtcgc tgacaatgtc ggtcacgtcc
     3721 ttgccgctgt catccaccgc gtgcgctatc ttgcgcggct tctcaactgt cgtgattgca
     3781 agcttcgctg gcgggataac aaaacgttca tcaacgaaga tatctgtacc cgccggatga
     3841 tcgacggtca ttaggttcaa ttgatcgtag tagtaagttt cccaaagctc cgctgttatg
     3901 cgcaggtcat agaaaccatc acgaggcaaa agttcatcgc cgccgatctt gtaccactct
     3961 tctgtagctt cgactctcgc tgtgccaatt gtattgatgc gtagaccgat tgccgaaccc
     4021 cagggcacgg catctttcac aaacttcatc tc
//
LOCUS       DASUEX010000151         2488 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1518411, whole genome shotgun sequence.
ACCESSION   DASUEX010000151 DASUEX010000000
VERSION     DASUEX010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1518411"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..579
                     /locus_tag="VFV61_02530"
     CDS             <1..579
                     /locus_tag="VFV61_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899376.1"
                     /translation="KKYDKDSDQRIHYDEMKVEPQMQEHFGWLDANSDGYLDRAEYDF
                     VRTGTSTSGHGITAVRLGGNGDLTPTNVAWRLTKSYPNIPAPLIYRDVMYLMKEGGIV
                     TSINPATGEVLKMGRTPEALEDYSASPVAGDGKIFMVSGSCKVTVLKAGAQWEVLATN
                     DLDEECWATPAIAGNNLFIRTRNTLYSFATTK"
     gene            complement(745..1230)
                     /locus_tag="VFV61_02535"
     CDS             complement(745..1230)
                     /locus_tag="VFV61_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899377.1"
                     /translation="MNSKRITLALIISLAIAALAGLGGAPLKVRGQENKSINQLIGTW
                     EVTVTPNGGAPIIDFATFHESGGVTNIDPDPNLSAGLGTWERLGPNRFASTFVHFLSD
                     HGTPLGLLKVNAEATYDPKTDTISGPFRTDVVIGGNVVQSICGTVALRRVGVEPLEDC
                     P"
     gene            complement(1256..1507)
                     /locus_tag="VFV61_02540"
     CDS             complement(1256..1507)
                     /locus_tag="VFV61_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899378.1"
                     /translation="MKKTKTTEITVETNELFIIKRRDRLAPGQCEACDAWVQMVTLAE
                     AAVIARVSELTADQIKARSLHSTNTPEGQVLICLNSLLA"
     gene            1728..>2488
                     /locus_tag="VFV61_02545"
     CDS             1728..>2488
                     /locus_tag="VFV61_02545"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEX4899379.1"
                     /translation="MSKSQQIKRFYEFGPFLVDASERVLLREGEPVMLPPKLFDTLLA
                     LVERSGHIVEKAELMETVWPGTFVEESNLSSSVSLLRKTLGQAEDGKPYIETVPRRGY
                     RFAPAVELTDETADLIVGRRTRVHVVTREEEETSDQDVNGDVRSKSTGSSMPARSGNE
                     RMFATAAIQAQENTGMESVLATTSPVQAVSPRLVFIFAISAVVVLGAGIFLAVRYFGR
                     NEGATAREPFGKIKLTRLTTTGTAHDAAISPDGKF"
BASE COUNT          574 a          680 c          688 g          546 t
ORIGIN      
        1 aagaaatacg ataaggatag cgaccaacgg atccattatg acgagatgaa agttgagcca
       61 cagatgcagg agcattttgg atggctcgac gccaacagcg acggctatct cgatcgcgcg
      121 gaatacgact tcgttcgcac cggcaccagc acttcgggac atggcattac ggctgtacgg
      181 ctgggtggca acggtgatct cacaccgacg aacgttgcct ggcgactgac gaaatcctat
      241 ccgaacattc ccgcgccgct gatctatcgt gacgtgatgt acttaatgaa agaaggaggc
      301 atagtcacga gtataaatcc cgcaactgga gaggttttga aaatggggcg cacgcccgag
      361 gcactcgagg attactccgc atccccggta gcgggcgatg gaaagatctt catggtaagt
      421 ggaagctgta aggtgacggt gctaaaagca ggcgcacaat gggaagtact ggcaacgaac
      481 gaccttgacg aagagtgctg ggctactccg gcgattgccg gcaacaacct attcattcgc
      541 acacgcaaca cgctctactc cttcgcaact acaaaatagc ttcgcagacg cgggaagttc
      601 tcgcggctat gtaggccggg tgtatcgcgc gcaagatatg tcaaccagcc ccagcaggga
      661 cctgccatgc gcccgcgttg tgcgccttcg ctgacttgcc gctaatcgac tgaagggtcg
      721 gcaaaggcgc accacactcg aatcctatgg gcagtcttcc aatggctcga cgccgacgcg
      781 tctgagagcc accgtgccgc aaatggactg gacaacattc ccgccaatca ctacatcggt
      841 ccgaaatggg ccgctgattg tgtcggtttt ggggtcataa gtcgcctcgg cattaacctt
      901 caacaagccg agcggagttc cgtgatcgct cagaaaatga acgaacgtgc tggcgaagcg
      961 attgggacca agtcgctccc aagttccaag gcccgcgctt agattcggat ccggatcgat
     1021 gttcgtgacg cctccgctct cgtgaaaggt cgcgaagtct atgatcggag ctcctccgtt
     1081 tggcgtcacg gtaacttccc aagtccctat caattggttg atagatttat tctcctgccc
     1141 gcggaccttg agaggtgcgc cccccaaccc tgctagcgcg gcaatcgcca agctaataat
     1201 cagagccaaa gtgattctct tgctgttcat ggtgtttctc cttttcatgt cattgttaag
     1261 cgagtaatga gttgaggcag atcagaacct gcccctcagg tgtgtttgtt gagtggagag
     1321 aacgagcctt gatctgatcc gcggtcaact cgctcacgcg ggctatgacg gctgcttcag
     1381 ccagcgtgac catctggacc cacgcatcgc aggcttcaca ttgaccaggg gccaatcgat
     1441 cacgcctttt gatgatgaac aactcattcg tctcgacagt gatctcggtt gtctttgttt
     1501 tcttcatgac atctccgcac gctgaaagtt tcctttgagg tgcattggcg ttgttccttg
     1561 ctggcccaaa cgcgccgcag tacaattcgg cctgttgcca cgaccttccg cacctcaacc
     1621 accacggcgt gctgtcgtca acgagcgccc atcatgctca ggcgcacccc atgaagtctt
     1681 gaggaaactc ttgggaagtt ctgggaaatt tcctggcaag ttctaacgtg agcaagagtc
     1741 aacaaataaa gcgcttttac gagttcgggc cttttctcgt cgacgcctcg gagcgtgtgc
     1801 tgctgcgcga gggcgagccc gtgatgctgc cgcccaagct cttcgacact ctccttgcgc
     1861 tcgttgagcg cagcggacac atcgtcgaaa aagccgagtt gatggagacc gtgtggccgg
     1921 gcacatttgt cgaagagagc aacctttcat ccagcgtttc gcttttgcgc aagactttgg
     1981 gccaagccga ggatggcaag ccttatatcg agacggtacc gcggcggggc tatcgatttg
     2041 cgcccgcggt ggagttgacg gacgaaactg ccgatttaat cgtgggcagg cgcacgcggg
     2101 ttcatgtggt gacccgtgaa gaagaggaaa cttcagatca ggatgtaaac ggagatgtta
     2161 gaagcaaatc aacaggcagc tccatgcccg cgcgtagcgg caacgaaagg atgtttgcga
     2221 ctgctgcgat tcaagcccag gaaaacactg ggatggaatc tgtactcgca acgacaagtc
     2281 ccgtccaggc tgtgagcccc aggctagttt tcatattcgc catttcagcc gtcgtcgtat
     2341 taggagcagg cattttcctg gcagttagat attttgggag gaacgagggt gcgactgcac
     2401 gagagccttt tgggaagatc aaactgacac gccttaccac cacgggcaca gcacacgatg
     2461 ccgctatctc tccagatggc aaattctt
//
LOCUS       DASUEX010000152         3600 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_831591, whole genome shotgun sequence.
ACCESSION   DASUEX010000152 DASUEX010000000
VERSION     DASUEX010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3600
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_831591"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..1316)
                     /locus_tag="VFV61_02550"
     CDS             complement(60..1316)
                     /locus_tag="VFV61_02550"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX4899380.1"
                     /translation="MCKRNFAILILIVSVGSLAYADNWPQWRGPAMNGVSAEKNLPLR
                     WTTEENIAWKLALPGLSGSTPIVWRDRIFLNVAEGDDLYLWCIDRPKGVLAWKKQLGT
                     GNVKMRKQNMSSPSPVTDGRNVYVMTGTGVLKGFDFSGKEIWSRDLQREYGAFGLNWG
                     YASSPLLTGDALYIQVLHGMKTDDPSYVMRVDKTSGKTVWKVDRPTEAIKESPDSYTT
                     PALLRYGKNAELVVTGGDCVTGHDLATGKELWRANGLNPDNNPFYRIVASPVIFNEII
                     YAPTRVRPLLALKAGGRGDITTSHLLWSTPNGPDVPTPITDGQYFYIVNDRGIMWCLD
                     AKTGAEIYAQQRLQPGSYSASPVLADGKIYVTNEDGLTSVIRAGAKFEILAENALHDY
                     CLSSPAISDGQIFIRTTQALYCIGKK"
     gene            complement(1518..>3600)
                     /locus_tag="VFV61_02555"
     CDS             complement(1518..>3600)
                     /locus_tag="VFV61_02555"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX4899381.1"
                     /translation="YWKKYRTAPKAFVMLQAAQELWQSRFGKLTSLRVFPKRDRKLAE
                     AQPIFEQTLRATLEPLKAGFTILPVRAQGLAASRGATDFGEYFLYFSFFLVISALLLT
                     ALFFKLGVEQRVREIGVLQAMGFSAGRVRAIFLGEGIILALVGSALGLVGALAYGQLI
                     MIGLRTWWVDAVGTTQLSLHVRPMSLVLGGLGGIVAALVCVVLTLRKLGKQSTRSLLS
                     GQAEPDDEGKRRFVKLGFSLRFLLGIAFSVFGTALLVTAGLKLIGETTGFFGGGVLLL
                     IGLLIWQSAWLHRAEAKPLQGKGWWPLSRLGIRNASSRPSRSVLCIALIASAAFIIVA
                     VDAFRHREGTTTLERKSGSGGFSLLAESLVPIVHDLGTDAGREALNLTLDASSPLAGV
                     TFERFRVRPGDDASCLNLYQPANPKIIAPRDSFLQSNRFAFSNSIEATAAEQSNPWLL
                     LNHKFADGAIPFIADANSATYVLHVKTGEDLVLPSGEHFRLVATLANSVLQSELIISE
                     ANFLRLFPNEEGYRLFLIDTPAREQADAVATTLEERLADYGVDVVPTGERLANFHRVE
                     NTYLSTFQMLGGLGLLLGTLGMAAVLLRNVLERRRELALLRAVGYNAGHLALMVIAEN
                     FFLLTCGLLTGTVCAIMAIAPVFFERGGRVPGISLGFLLLAVLISGLTASILATWATL
                     RSPLLPALRAE"
BASE COUNT          853 a         1004 c          987 g          756 t
ORIGIN      
        1 tgggcgttgc ccacggctat tagctccctc cgctgcgggg acgaactgta ctcgctaaat
       61 cacttcttac cgatgcaata gagggcctgc gtggtgcgaa tgaaaatctg tccatctgaa
      121 atcgccggcg aactgagaca ataatcgtgg agtgcgtttt ccgctagtat ctcaaacttc
      181 gccccggctc gaatcactga cgttaaccca tcttcattgg taacgtaaat ttttccatcc
      241 gccagaaccg gcgaagcgct gtagctcccc ggctgcaacc gttgctgcgc gtaaatctcg
      301 gctcctgttt tggcatcgag gcaccacatg atgccgcggt cattcactat atagaaatac
      361 tgcccgtcgg taatgggggt gggcacgtcc ggaccattag gagtcgacca gagcagatgc
      421 gaagttgtga tgtcaccgcg accgccggcc ttcagggcga gtaatggacg cacgcgcgtg
      481 ggcgcgtaga tgatttcgtt gaagatcacg ggcgaggcca caatgcggta aaagggattg
      541 ttatcgggat tcagaccgtt tgcgcgccag agttccttcc cggttgcgag atcgtgtcca
      601 gtgacgcagt cgccaccagt aaccaccagt tctgcgttct tgccatagcg cagcagggcc
      661 ggtgttgtgt aagagtcggg cgactccttg atggcctccg ttggccgatc gactttccag
      721 accgtcttgc cgctggtctt atctacgcgc atgacgtacg agggatcgtc ggttttcatt
      781 ccgtgcagca cctgaatata gagcgcgtcg ccggtcaata gcggggaaga ggcgtatccc
      841 caattcaagc caaaggctcc gtattcacgt tgcaggtcgc gagaccaaat ctccttgccg
      901 ctaaaatcga aacctttcag cacgccggtg ccagtcatca cataaacgtt cctgccgtcg
      961 gtaacgggcg aaggtgacga catgttttgt ttgcgcatct tgacgttgcc tgtgcccaac
     1021 tgcttcttcc aggccaagac ccccttcggg cgatcgatgc accagagata caagtcgtcg
     1081 ccttcggcga cattgagaaa aatacgatcc cgccaaacga tcggtgtcga accactcaag
     1141 cccggaagcg cgagcttcca agcgatgttt tcttccgtcg tccagcgcag cgggaggttc
     1201 ttctcagcgc tgaccccgtt cattgccggc ccgcgccatt gcggccagtt gtcggcgtaa
     1261 gcaagtgagc cgacactgac gataaggatg agaatcgcaa aattccgttt acacattgtt
     1321 gctcccaccg ctccggttaa ttcgaaaagg cacgctagtg attcaatacc attcgaggtc
     1381 tctcattctc accgcgcttc agcgcggtgt tcggattcga gagttaaccg gaaaccgttt
     1441 aaacggtttc cggttttaag gtgcctacgc gtcaccggac tgaagtccgg tgtgaaataa
     1501 gaacaacttg tcttttgcta ctccgcgcgc aaagccggca gcagcggtga tcttaacgtt
     1561 gcccaggttg ccaggattga tgcagtcagt cctgatatta ataccgccaa cagcaagaag
     1621 cccagagaaa tacccggcac gcgtccgccg cgttcaaaaa ataccggagc aattgccatg
     1681 atggcgcaaa cagtaccagt caacagtccg caagttaata ggaaaaagtt ctcggcgatt
     1741 accatcagcg ccagatgccc cgcgttgtaa cctaccgcac gcaagagcgc cagctcgcgg
     1801 cgacgttcca gcacattgcg caagagcacg gcggccattc cgagggttcc caagagcaac
     1861 ccaagacctc ccaacatttg aaaagtagag agataggtgt tttcaacgcg atggaaattt
     1921 gcgagccgct ccccagtagg cacaacgtca acaccatagt cggcaaggcg ctcctcgagt
     1981 gtggttgcca ccgcgtctgc ctgttcgcgc gcaggcgtgt cgatcaagaa gagccggtac
     2041 ccttcttcgt taggaaagag tcgcaagaaa ttcgcttcag aaattatcag ctcactttgc
     2101 agcacactat tggcgagcgt ggccaccaga cggaagtgtt cacccgaagg caagacgaga
     2161 tcttcaccag ttttgacatg cagaacgtag gtcgcggagt tggcgtcagc gatgaacggg
     2221 atcgcaccgt cagcaaattt gtgattaagc agtaaccacg gattgctttg ttccgccgca
     2281 gtcgcctcaa ttgaatttga gaaagcaaag cggttgcttt gtagaaaact gtcgcgcggc
     2341 gcgatgatct tcggattcgc tggctggtaa agattgagac agctggcatc atcgccgggg
     2401 cgcacgcgaa agcgttcgaa agtaacgcca gcaagcggcg aggaagcatc gagggtgaga
     2461 ttgagcgctt cgcgcccggc atctgtgcca agatcatgca cgattggcac gagagactcg
     2521 gccagcaagc tgaacccgcc gctgcctgac ttgcgctcga gtgttgttgt tccttcgcga
     2581 tggcggaagg cgtctacggc cacaatgata aaggccgcag aagcgatcag ggctatgcac
     2641 aagacgctgc ggcttgggcg tgaagatgcg ttgcggattc ctaaccgcga gagcggccac
     2701 caaccttttc cctggagcgg cttcgcctca gcgcgatgca gccaggcaga ctgccagatc
     2761 agaagtccga tcaatagcag taccccgccg ccaaagaaac cagtggtctc gccgattaat
     2821 ttcaaaccgg cggtaactaa cagagctgtg ccgaatactg agaacgcgat tccgagcaag
     2881 aaacgaaggc tgaaccccag cttaacgaat cttcgtttcc cttcgtcgtc aggttctgct
     2941 tgacctgaca aaagactccg cgttgattgc tttcctaatt tccgtaaggt gagcactacg
     3001 cagacgagcg ccgccacgat gcccccaagg ccgcccagca caagtgacat aggcctgaca
     3061 tggagagata gttgagtggt gccgactgca tcgacccacc aggtgcggag accaatcatg
     3121 atcagttgac cgtaagcgag ggcgccgacc aggccgaggg cgcttccaac cagagcgaga
     3181 atgattcctt caccaagaaa tattgctctt actcgacccg ccgaaaaccc catcgcttgc
     3241 agtacaccta tttctctgac gcgctgctcc acaccgagct taaagaacag ggctgttaac
     3301 agaagcgccg agattacgag aaagaaactg aagtaaagaa aatattcgcc gaagtcggtc
     3361 gcgccgcgcg aggcggcgag tccttgggcg cgcaccggaa gaatcgtgaa acctgctttc
     3421 aagggttcaa gggttgcgcg cagggtttgt tcaaaaatcg gctgtgcctc cgcgagcttt
     3481 cgatcgcgct tgggaaagac gcgcagcgaa gttagtttgc caaagcgtga ttgccaaagt
     3541 tcctgagctg cttggagcat cacaaatgct ttgggcgccg tgcgatactt tttccaatag
//
LOCUS       DASUEX010000153         3190 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1519095, whole genome shotgun sequence.
ACCESSION   DASUEX010000153 DASUEX010000000
VERSION     DASUEX010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3190
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1519095"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            64..612
                     /locus_tag="VFV61_02560"
     CDS             64..612
                     /locus_tag="VFV61_02560"
                     /inference="COORDINATES: protein motif:HMM:TIGR02999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX4899382.1"
                     /translation="MTELLGEWREGDKTALERLTPLVYAEIRRMAHRLMQRERVGHTL
                     QTTALVNEAYIRLLGQQPVEWRSRSHFFAVVAQLMRHLLIDHARRRLYAKRGGDAQQV
                     SLNEATLMSDARAGELVALDEALGKLSQLDPRKGRVIELRYFGGLSLEETSEMLGVSV
                     MTVRRDWRAAKAWLYREMKAEG"
     gene            654..2744
                     /locus_tag="VFV61_02565"
     CDS             654..2744
                     /locus_tag="VFV61_02565"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX4899383.1"
                     /translation="MSYARWQKIESVLQEALDLPAASRAAYLSGACRGDADLHREATS
                     LIDALEHSPDFIEEPAIIKDARVLASDLPDPNIGRVIGAYNILEHLGSGGMGEVYRAE
                     DSRLGRSVALKILPDLLVSDATLFRRFQTEARAASALNHPNVLTVHEVGEVDGTHFIA
                     TEFIDGETIRELTDKGDLTIGEVLDICIQVGTALASAHAAGIIHRDIKPENIMRRSDG
                     LVKVLDFGIAKPTGKSLCGDFAPMPEAEAQYTEMGVVLGTVGYMSPEQARGLPVNQRT
                     DIWSLGVVLYELLARRAPFARETRMDTLVSILEHEPQPVFTMTTQTPALIRSLQQIVT
                     RSLTKDPGQRYQTVEAMLEELKECKKEFDHAILLGDETTGGWLSRTSITRSETTEHSP
                     QNIIEARTRLLPSPVTSATSRGASWKALGIGAAILLGALFLYGFYLRQVRERSTGLST
                     TASATKLYREMSEPERLAFVAGQEQRISAMLGDRQVPLDQDALMAIKGKVDSYVARKD
                     KTSNGVNGETLSEIYERATPYIPLIARSFEAQKVPVTVGIYLPMIEAEYHPCSVSPIG
                     AKGLFQFLPQTAMKYGVSPKEMCDAEKMTPAAAHYIADRMAELGEDSQSLTLVLLSYN
                     RGVGWVQNTLRELRGTDKFQRNFWTLLANRDKLDESFQNESAGYVPRFFAAAIIGENP
                     RVFELQTPPLSTLR"
     gene            complement(2777..>3190)
                     /locus_tag="VFV61_02570"
     CDS             complement(2777..>3190)
                     /locus_tag="VFV61_02570"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX4899384.1"
                     /translation="DTNLKGAWNFAKAVLRTMLRQETGGSILNISSISGTVGMPGQSN
                     YSASKAGMVGLTKALAKEVASRKVTVNALALGMVATDMAGALDEEYRAKILEQIPLGR
                     FAEAREVARIACFLLSDDARYITGQVIQVVGGLAI"
BASE COUNT          757 a          852 c          885 g          696 t
ORIGIN      
        1 tagaattccc tggtgcaaca agtcttaaag catgatccga ataaggtgag agcaggagac
       61 aatttgaccg aacttctggg cgagtggcga gagggagata agactgccct tgaaagactg
      121 acgccactgg tctatgcaga aattcggcga atggcacatc gtctcatgca acgcgaacgc
      181 gttggtcaca ctctgcagac taccgccctg gttaatgaag cttacattcg actattgggc
      241 cagcaaccag ttgagtggcg cagccgctct cacttctttg ctgtggtggc gcaactaatg
      301 cggcatctgc tgatcgacca tgctcggcgt cgcctttatg caaagcgcgg cggggacgct
      361 cagcaggttt ccctcaacga ggcaacgctg atgtctgatg ctcgtgcagg tgaattggta
      421 gcgctggatg aagcgcttgg aaaactgtcc caactcgacc cgcgaaaggg ccgggtaatc
      481 gaactgcgtt atttcggtgg actcagcctg gaggaaacct ctgaaatgct gggagtctct
      541 gtcatgactg ttagaaggga ttggcgcgcc gcaaaggcgt ggctgtatcg cgagatgaaa
      601 gctgagggct gattcgatcg aagtgcgccc actcgtcaat cgtcaataac cttatgtcct
      661 acgctcgttg gcaaaagatc gaatcagtct tacaggaggc cttagacctt cccgcggcct
      721 cgcgcgccgc ttacctcagc ggtgcgtgtc gcggtgatgc cgacctgcat cgagaagcca
      781 catctctaat cgacgcgctg gagcacagtc ccgatttcat tgaggagccc gcaatcatca
      841 aggacgctcg cgtgcttgcc agtgacctgc ctgacccaaa tatcggacgc gtcatcggag
      901 cctacaatat cctggaacac ctgggttccg gaggcatggg tgaggtttat cgtgcagagg
      961 attcgcgtct tggccgctcc gtcgcgctga agattttgcc agacttactg gtctctgacg
     1021 caacgctgtt tcggcgtttc cagaccgagg cgcgggctgc gtccgccttg aatcatccga
     1081 atgtcctcac cgttcatgag gtcggggaag ttgatggaac ccatttcatt gccaccgagt
     1141 tcatcgacgg tgaaaccatc cgggaattga cagataaggg tgacctcacg attggtgaag
     1201 tcctggacat ctgcattcag gttggcactg ctctggcaag tgcacacgcg gccggaatca
     1261 ttcatcgcga catcaagccc gaaaacatca tgagacgaag cgacggtttg gtgaaggtgc
     1321 ttgatttcgg aatcgcaaag ccaaccggca aaagcctctg cggagatttt gcgccaatgc
     1381 ctgaagctga agctcagtat accgaaatgg gagtcgtgct cggcacagtt ggttacatgt
     1441 cccccgagca agctcgcggt ttacccgtca atcagcgaac agatatctgg agtttgggag
     1501 ttgtgcttta tgagttgctg gcccggcgag ctccatttgc gcgggaaacg cgaatggata
     1561 cgcttgtgag cattctcgaa cacgaaccac aaccggtctt tacgatgaca acgcaaacgc
     1621 ccgctttgat tcgcagcttg caacaaatcg tgactcgcag tctcaccaaa gatccgggcc
     1681 agcgatacca gacagtggaa gcgatgctcg aagaactcaa ggagtgtaag aaagagttcg
     1741 accacgcaat tctcctcggc gatgaaacca ccggcggatg gctcagtcga acttcaatca
     1801 cgagatcgga aaccacagaa cactctcctc agaatattat cgaggcgaga acacggctgc
     1861 ttccctcgcc agtgacgtcg gcaaccagta ggggagcaag ctggaaggcc cttgggatcg
     1921 gcgccgcaat attgctgggg gctctcttct tgtacgggtt ttacctgcgc caggtgcgag
     1981 agcgttcgac gggcttatcc acgaccgcaa gcgcgacgaa gctttacagg gagatgagcg
     2041 agccggagag acttgcattc gtagcggggc aagagcagcg catttcggca atgctgggcg
     2101 accggcaggt cccgcttgat caggatgcgc tgatggcaat caagggaaag gttgattcct
     2161 acgtcgcgcg caaagataag acttcaaacg gagtcaacgg tgaaaccctc tcggagatct
     2221 acgaacgagc tactccgtat atcccgctga ttgcgcgttc ctttgaagcg caaaaagtgc
     2281 ccgtcacggt cggcatctac ttgccaatga ttgaagctga ataccatcct tgttctgtta
     2341 gtcccattgg cgctaaggga ttgttccagt ttctgccgca gacagccatg aagtacggcg
     2401 taagcccaaa agaaatgtgt gatgctgaga agatgacgcc ggcggcagcg cattacatcg
     2461 ctgacagaat ggctgaactt ggtgaggact ctcaaagttt gaccttggtt ttgttgagct
     2521 ataaccgtgg tgttggttgg gtgcaaaaca cgctcaggga gttacgagga acagacaagt
     2581 ttcaacgaaa cttctggacc ctcctcgcca accgggacaa attggatgag tcgtttcaaa
     2641 acgagagcgc tgggtacgtt cccagatttt tcgcggcggc gatcattggt gagaacccgc
     2701 gcgtctttga attgcagact ccgccactgt caaccctcag atagtctcag caccccaaga
     2761 cttgagactt aagaaattag atcgccaacc caccaaccac ctggatgacc tggccagtga
     2821 tgtaacgcgc atcatcagac aagagaaaac aggcaatgcg tgctacctcc cgcgcttcag
     2881 caaaacggcc caatggaatc tgctccagaa ttttggctcg atactcctca tcgagtgctc
     2941 cagccatatc ggtcgcaacc atgccgagcg cgagcgcgtt gacggttact ttccgactcg
     3001 ccacttcctt tgccagggct ttggtcaagc ccaccatgcc ggcttttgaa gcagaatagt
     3061 ttgattgccc tggcatacca acggtgccgc taatcgaact gatgttcagg atggagccac
     3121 ccgtttcctg ccgcaacatt gtgcgcagta ccgctttggc aaaattccag gcgcctttga
     3181 gattggtgtc
//
LOCUS       DASUEX010000154         3393 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_487178, whole genome shotgun sequence.
ACCESSION   DASUEX010000154 DASUEX010000000
VERSION     DASUEX010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3393
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487178"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2685
                     /locus_tag="VFV61_02575"
     CDS             <1..2685
                     /locus_tag="VFV61_02575"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX4899385.1"
                     /translation="TLDEDRLRRFEQEAYAASGLNHPHILTIYEIGEIEHRRFIAMEY
                     VEGETLRHHISQAATTSSPDGVAAGSGLKLGHALDIAIQIASALAASQTAGIAHRDIK
                     PDNIMVRRDGYIKVLDFGLAKLTERPETTDTEAPTRALVNTSPGAVMGTVNYMSPEQA
                     MAQVVDARTDIWSLGVVLYEMVTGKTPFSGPTPSHVIVAITDKDPAPLARYLSDVPEA
                     LEWIVTKALTKDRDDRYQTAREILTDLRRLKQRLDAGVEMERSVPPDTISGGHSITTA
                     DTLAGRTLSAFPGTSTQVGATTTSSSAEHLTSSVRSHKLAWGIGAVALIAVVIGGSVL
                     VAKLRTKETVRPFAKVRFSQLTSTGKATVATISPDGKYVVHVVSDGTQSSLWVRQTAT
                     ASNVQIVPPSETRYVGVTFSMDGNYVYYVVYEKSAAIGFVYQIPVLGGTPRKIIEDVD
                     TPITFSPDGRRFAWIRNFPRAGETALFTANSDGTGEKKIASLQRPERFMAGISIGPSW
                     SPTEEVVAAPAAGPENGSDVGRIVAVDLKTGAQKEVSSHRWPFLQQVVWMRHGEGIIL
                     TAQEAQRGPNQLWHVGYPDGVVERITNDLNNYNGVSLSADGGTIATVQSQTSSSVWLA
                     PNSRAETAVKISSGTNEGGGGVALLPDGRLVYALAGSGSSDLLLTDPNGTSSQPLTGN
                     SALNGLPSVSPDGRFIVFVSNRTGGPHVWRMNSDGSNLKQLTNGIAEIFPVVSPDNKW
                     VLYQNLSDLRLWRVSIDGGEGNQLIDKLASQAVISPDGKLVACRYREQELSPFQLAIL
                     AFNDGKTLKTFDLPPSAFGTPNLDWTADGKDVLYVDTRAGIGNVWRQPIDGGAAKQLT
                     FFNTDQIFAFDLSTDGKTMALARGNVSNDVVLIVAANQ"
     gene            2704..3156
                     /locus_tag="VFV61_02580"
     CDS             2704..3156
                     /locus_tag="VFV61_02580"
                     /inference="COORDINATES: protein motif:HMM:NF025227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 family protein"
                     /protein_id="HEX4899386.1"
                     /translation="MNSKSGIAIKYGLLITLVVVVWVILTHFVVPLPPTSQVNIVAPV
                     LFNLAAIIALSLGIKARNANGDMDFKEGLKTGLGISLVYAISACLFFLLLFLIVGPSI
                     MANEPMAADSPVWRVALAAFAGMFFGSLILGLVYSTIISFFLARRYQR"
BASE COUNT          744 a          934 c          942 g          773 t
ORIGIN      
        1 acgctcgacg aggatcggct gcgacgtttc gaacaggaag cctatgcggc ctccggactc
       61 aaccatccgc atatcctgac gatctacgaa atcggagaga ttgaacaccg gcgctttatc
      121 gccatggagt atgtggaagg cgaaacccta cgccatcaca tttcccaggc tgccacgacg
      181 tccagtccgg atggagttgc tgctggttcg gggttgaagc ttggtcacgc gctggacatt
      241 gcgatccaga ttgcttccgc cctcgcggct tcgcaaacag ccggcatcgc ccatcgcgac
      301 attaagccgg acaacatcat ggttcggcgc gacggttaca ttaaggtgct ggacttcgga
      361 ctcgcaaagt taaccgagcg tccggaaacg accgatacag aagcgccgac tcgagccttg
      421 gtcaatacca gtcccggcgc ggtgatgggc acggttaact acatgtcgcc agaacaagcg
      481 atggcgcagg tagttgacgc gcgcaccgac atctggagtt tgggcgtcgt cctttatgaa
      541 atggtgaccg gtaagactcc tttctccgga cccactccga gtcacgtgat tgtcgccatc
      601 accgataaag atccagcgcc gctcgcgcgc tacctcagcg acgttccgga agcgctcgag
      661 tggatcgtta ccaaggcact caccaaagat cgcgacgatc ggtatcaaac ggcgcgggag
      721 attcttactg acctgcgacg gctcaaacag cgactcgacg ccggcgtgga aatggaacgg
      781 tctgttccac ctgacacaat ctccggcgga cactcgatca ctactgctga tactctggcc
      841 ggccggacgt tgagcgcctt cccgggaact tctactcaag tcggcgcgac aaccactagc
      901 tccagcgccg agcacctgac gtcctcggtg cgcagtcaca aactcgcgtg gggcatcggc
      961 gctgtggcct tgatagccgt tgtcattggc ggcagtgtgc tggtagcaaa gcttagaact
     1021 aaagaaacag tgcgaccttt tgccaaagtt cgtttctcgc aattaaccag tacgggcaaa
     1081 gccactgtcg ccacgatttc tcctgacggg aaatacgtcg tccacgtagt aagcgatgga
     1141 acgcagtcga gtctgtgggt gcgtcagacc gccaccgcaa gcaatgtgca gattgttcca
     1201 cccagcgaga cgcgttacgt tggcgtcact ttttccatgg acggcaacta cgtttactac
     1261 gtcgtttacg aaaagagcgc cgcgatcggc ttcgtttatc agattccagt tttgggcggc
     1321 accccgcgga aaattattga ggacgtcgac acccccataa cgttttcacc ggacggcagg
     1381 cggttcgcct ggattcgcaa ctttcctcgc gccggagaaa ccgcgctctt tacagcgaac
     1441 agcgacggca ctggagaaaa gaagattgct tcgctgcaac ggcccgaacg ctttatggca
     1501 ggtatttcga tcggcccatc gtggtctccc accgaggagg tggtagcggc tcctgctgct
     1561 ggcccagaaa acgggtcaga cgtaggtagg atcgtcgcag tcgatttgaa gaccggcgcg
     1621 caaaaggagg tctcctctca ccggtggccc ttcctgcagc aagttgtctg gatgcgccat
     1681 ggtgagggaa taatcctgac cgctcaggaa gcccagcgcg gcccgaacca actatggcac
     1741 gttgggtatc ccgacggggt agttgagaga atcacaaacg atctgaacaa ctataacggc
     1801 gtcagtctca gcgctgatgg cggcaccatt gccactgtgc agagccaaac gagttcaagc
     1861 gtgtggttgg ccccaaacag tagagctgag accgcagtca agatttcgag cggcacgaat
     1921 gaaggcggcg gcggcgttgc gttgctgcct gacggccggc ttgtttatgc gcttgccgga
     1981 tcgggcagca gcgaccttct gcttaccgac ccaaatggta ctagctcgca accgctgact
     2041 ggtaattcag cattaaacgg actgccctcg gtttcgccag atggacgctt cattgtgttt
     2101 gtttcaaatc gcactggcgg gcctcatgtc tggcgcatga atagtgacgg cagtaacctg
     2161 aagcagctta ctaatggtat agctgagata tttcctgtcg tctcgccaga caataagtgg
     2221 gtgctttatc aaaacctaag tgacttgcgt ctctggcgcg tatccatcga cggcggtgag
     2281 ggaaatcaat taatcgataa actggcaagt caggctgtta tctcaccgga cggaaagctg
     2341 gtggcttgtc gttatcgcga gcaggagctg agtccatttc agttggcgat tctcgccttt
     2401 aacgatggca aaaccctgaa gacctttgat ctcccgccct cagctttcgg gacaccaaat
     2461 ctcgactgga ctgcggatgg caaggatgta ttgtacgttg atacgcgtgc cggtattgga
     2521 aatgtctgga gacaaccaat tgatggtggt gccgcgaaac aattgacctt ctttaatact
     2581 gatcagattt ttgctttcga tctttcgacg gatggcaaaa cgatggcgct ggcccgcggc
     2641 aatgtctcta acgacgtggt tttaatcgtt gctgcgaacc agtaagatcg cgaatcgtaa
     2701 atcgtgaata gtaaatcggg aatcgcaatc aaatacggcc tactaataac gttagtagta
     2761 gttgtgtggg tgatcctcac gcactttgta gttcctttgc cgcccacatc gcaggtgaac
     2821 attgtggcgc cggtgctttt caacctggcg gccatcatcg ccctctctct cggtatcaag
     2881 gctcgaaatg cgaacggtga catggacttc aaggaaggcc tgaaaaccgg cctggggatt
     2941 tctctcgtct acgctatctc tgcttgcctc tttttcttgc tcttgttcct gatcgttgga
     3001 ccctcaatca tggccaacga accgatggcg gcagactcgc cggtgtggag agttgcgctc
     3061 gccgctttcg ccggcatgtt cttcggctca ttaatcctcg gattggtcta ctccacaatc
     3121 atttccttct ttcttgctcg gcgctatcaa cggtgaccgg ggagcgggcg acgctgtcgg
     3181 ctttggggtc aaccggcacc ttgcacgcaa cgcatcttct aatgtaagaa aaagctaacc
     3241 gccaaggtgc cgagagcgcc gaggatgccg ggaagctttg gagtaagaga atgaaggttc
     3301 actcagccag cttgtgcgtt tgttctctcg gcgtcttggc gttctcagcg ccttggcggt
     3361 aaagttcagc cctttaattc ggttggtaac cct
//
LOCUS       DASUEX010000155         2436 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_717421, whole genome shotgun sequence.
ACCESSION   DASUEX010000155 DASUEX010000000
VERSION     DASUEX010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_717421"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="VFV61_02585"
     CDS             complement(<1..278)
                     /locus_tag="VFV61_02585"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX4899387.1"
                     /translation="MASQQTGLATHDLSAKYFARASRLSPGGVHSPVRAFKGVGGEPI
                     FFRSAQGATMTSVDGRQYIDFCQSFGPLMLGHRDEEVSAVVEEAVATA"
     gene            complement(300..1256)
                     /gene="hemB"
                     /locus_tag="VFV61_02590"
     CDS             complement(300..1256)
                     /gene="hemB"
                     /locus_tag="VFV61_02590"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF012702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEX4899388.1"
                     /translation="MKRLMRLRATPHLRDLCAETEFNRAQLIQPLFVVEGLEGEEPIA
                     GLRGNVRHGTSSLLRQIEHDLADGVTQFLLFPVPGEKKEKGFKHQFTVAAIAEIRRQF
                     GSGLCLWIDTCLCSYTSHGHCAVLDGSRIDHPATLAELANAAVAFAEAGADGISPSDM
                     IDGRVGHIRAALDERLMDLVPIMSYSTKFASNFYGPFRNAADSAPQFGDRRHYQLDVR
                     SRSEAIAASKRCAEEGADLLMVKPGLTSLDLIAPIQEQTGRRVGAYQVSGEYAGLALL
                     DEQGLINFEQALLETWHVFKRAQAQFIITYGARYARRLGIGN"
     gene            complement(1253..>2436)
                     /locus_tag="VFV61_02595"
     CDS             complement(1253..>2436)
                     /locus_tag="VFV61_02595"
                     /inference="COORDINATES: protein motif:HMM:NF013538.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899389.1"
                     /translation="LVRSERWEVADRSGRLRILTSSPRRSYNLEAFLPQVLPAKFDAL
                     EFVPVRGNVPTRVRKLWQPDIDGLVVAKAALDRLLEAEQAEFADTSADLRQALGQCRW
                     MVLPLRANPAAPGQGALAIETRRERKDLQDLLAAINCAETFQAVVREREILRAFGGGC
                     HQKIGVSVLHRPYGEITSLRGLADDGTILSNYSLYSSRPQPPRVFREQMWPLEPSQAD
                     WFGRERIPATLPDKPAAFWISKAEALPNGVSLSPSQIVWASGLHTWRRLAQRGVWVNG
                     CAESLGEQEDARIENLAGSEREWFKLTHADGYAGNGTKTLATYRLIPKNDNPQFKDQT
                     YFFWTSGSGFERALTLNPWLKQMTHFCGPGNTQRVLLRNGVEPFVFLDHAQWLEEMRL
                     S"
BASE COUNT          549 a          753 c          648 g          486 t
ORIGIN      
        1 caggcagtag ccactgcttc ttccacaacg gcgctcactt cttcgtcgcg atgaccgagc
       61 atcaaaggac caaagctctg acagaagtcg atgtactgcc gcccgtccac ggatgtcatg
      121 gtcgcgcctt gagcgctgcg aaagaaaatt ggctcgccgc caacgccctt gaaagcccgg
      181 acgggactgt gcacgccgcc gggagacaga cggctggcgc gcgcaaaata tttcgctgaa
      241 agatcgtgag tcgctaaacc tgtctgttgt gaagccatat ttaggttgat tcgaaacgac
      301 taattgccga tgcccagtcg ccgcgcatag cgagcaccat acgtaataat gaactgggct
      361 tgcgcgcgct tgaacacgtg ccaggtctcc aataatgctt gttcgaaatt gattaaaccc
      421 tgttcgtcca acaacgccag cccggcgtat tcaccgctaa cctgataggc tccgacccgc
      481 cgcccggttt gctcttgaat cggagcaatc aggtcaagcg aagtcaatcc gggcttgacc
      541 atcagcaaat cagctccctc ttccgcgcag cgcttgctcg ccgcaatcgc ttcgctgcgg
      601 ctgcgcacat cgagttgata atgacgtcga tcaccaaact gcggcgcgga gtccgctgca
      661 ttccgaaacg gcccatagaa gttgctggcg aactttgtgc tgtagctcat tatcgggacc
      721 aaatccatca gtcgctcgtc gagtgccgcc cgaatgtgtc cgacgcgtcc atctatcatg
      781 tcgctcggac tgatgccgtc ggcgccggct tcggcgaaag caacggcggc gttagccagc
      841 tcagccagag tcgcggggtg atcgatgcgc gaaccgtcca gcaccgcaca gtggccgtga
      901 ctcgtgtaag agcagagaca ggtatcaatc cataaacaca aaccgctccc aaactggcga
      961 cgaatctcag cgattgctgc aaccgtaaac tgatgcttga aacccttctc ctttttctcg
     1021 cccgggacag ggaaaagtaa aaactgggtt acgccatccg cgagatcgtg ctcaatctgt
     1081 ctcagcagcg aagaggtgcc gtggcggacg ttaccgcgta atccagctat gggctcctcg
     1141 ccctcgaggc cttcaaccac aaacaaaggt tgaatcaact gcgcccggtt gaattcagtc
     1201 tcggcacaca ggtcgcgcag gtgcggcgtt gctcgaaggc gcatgagacg cttcatgaaa
     1261 gtcgcatctc ctccagccat tgagcgtgat caagaaatac gaacggctcc acgccattcc
     1321 ttaagaggac gcgttgcgtg ttcccgggcc cacagaagtg cgtcatctgt ttcaaccagg
     1381 gattgagcgt cagcgcccgt tcgaagcctg agccgctcgt ccagaaaaag taggtctggt
     1441 ccttaaattg gggattatcg ttcttcggaa tcaaacgata ggtggccagt gtcttcgtgc
     1501 cattgccggc gtagccatca gcgtgcgtga gcttgaacca ttcacgttcg gaacccgcaa
     1561 ggttttcaat gcgcgcgtct tcctgttcac ccaaactctc agcgcaaccg ttgacccaaa
     1621 ccccacgctg cgccaggcgg cgccaggtgt gcaggccact ggcccagaca atctgcgacg
     1681 gtgacaatga cactccgttg ggcaacgcct ctgctttcga gatccaaaac gctgccggct
     1741 tatcaggcag cgtcgccgga atccgctcgc gcccgaacca gtctgcctgc gaaggttcaa
     1801 gcggccacat ctgttcccgg aaaaccctgg ggggctgtgg tctcgaggag taaagcgagt
     1861 agttactcaa aatcgttccg tcatcagcga ggccgcgcaa agacgtgatt tcgccataag
     1921 gtcggtgcaa cacactaacg ccaatcttct gatggcaacc gcctccaaac gcgcggagaa
     1981 tctcgcgctc ccgcaccacc gcctgaaagg tctccgcgca attgatcgca gcaagtagat
     2041 cctgcaaatc cttgcgctcg cgccgggtct cgatcgctaa agcaccctga ccgggagccg
     2101 ccggattagc gcgcaacgga agcaccatcc aacgacattg cccaagtgct tgtcttaaat
     2161 cggcgcttgt gtccgcgaac tctgcctgtt ctgcttccag cagccgatcc agcgcagctt
     2221 ttgcaacaac caatccatca atatcgggct gccacagctt ccgtactcgt gtcggcacgt
     2281 tgccgcgcac aggcacgaac tccaaagcgt cgaattttgc cggcaatacc tgaggcagaa
     2341 atgcttcgag gttgtagctc cggcgcggcg aagaggtaag gatgcgcagt cgccctgagc
     2401 gatcggcaac ctcccaacgc tcactacgaa ccagca
//
LOCUS       DASUEX010000156         3764 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_947389, whole genome shotgun sequence.
ACCESSION   DASUEX010000156 DASUEX010000000
VERSION     DASUEX010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3764
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_947389"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1174)
                     /locus_tag="VFV61_02600"
     CDS             complement(<1..1174)
                     /locus_tag="VFV61_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899390.1"
                     /translation="MRHVLDPSPEQLAMPESTRGSAPSSKIIRWERLSWLAALALGFI
                     QALGRRHESADGLAYMGPDGISYLDIGDAYMRGDWYVAVNAMWSPFYSWLLGIWLHLF
                     KPSPFWEFTVVRLLNFLIYAVALFSFAYFLRALRRDRELRVSGVALPEWSWLVFGYTI
                     FIWTSLFMNRVWRTSPDLLVAALIFLACGLLLRIRAAPNRWQLFAALGIVLGVGFLSK
                     TIMFPLALVFILVAHLLARRAAEFKPALLRALIALLMFLTLATPFVILLSRAKHRLTI
                     GDSARLNYGWYVNGTTRYTHWQGEPTGSGTPVHPTRKIFATPAVYEFSAPPGISYPPW
                     YDPSYWYEGVTPRFVFRQQLAAIARNLTWLYGFLFYRFFLGALTFALFVLLYQSGRW"
     gene            1349..3157
                     /locus_tag="VFV61_02605"
     CDS             1349..3157
                     /locus_tag="VFV61_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899391.1"
                     /translation="MQTDKKTRWRWGVIAALAITLLSLFPQCYLWWERGRQWNGSQAF
                     FYTDEPAYAAYINALIDGRPRRNDPYTGRDDRPAAPLPESLFSIQFIPAYFIALPARV
                     LGLSTATAFILVTPIVAFTTALTLFWLLALITDDSRAAAAFVPLVLCAGLLVAGNGVV
                     RPFFGQQATLVYLPFLRRYSPAVVFPCFLIFFAFVWRILTTTSRQLRLLYVLGAAAAF
                     TVCLYGYFYLWTAALAWPALVACLWFTARPDEWRAGLRSIALLTAVILTVSVPYSILL
                     FRRAPTMDLAQALVRTHTPDIWRASEALALLLVIVVVVAILARRSSRQDPRILFTLAF
                     ALLPFVPFNQQVITGRSLQPMHYEQFVVTYTTLIALALVIVWLRSGRVEQSPVGGLLL
                     LTISLFGYGWGLGETWIATRRFAYVNSLRDEARPVGLRLRELAKPPVIDQQNPLEVVF
                     APNLGRSDNLPTTAPQAVLWAPHMFVFSGVAAAENKERFFQFLYFSGVGVEEFSHYEN
                     GFVKYAIFGWERANEKLTANFRPITAEEIALEAKNYADYIANFDSTRARRPTLAYLLL
                     DANQPINLSNLERWYTRDNGQRIGRYLLYRLTPRTT"
     gene            3154..>3764
                     /locus_tag="VFV61_02610"
     CDS             3154..>3764
                     /locus_tag="VFV61_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899392.1"
                     /translation="MSQSVVPRLNIEWRLGLIVAIAMSVLALVPQFHLWAVRSSDWQG
                     TYVTFDFDEVAYASYLNALIDGRPRRNDPYTGRDVTARQPLPESLHSIQFVAAYALAL
                     PARALGLSAGATFVIVRALAAFASTLALFWLLGLLTGDSRVAAAGTIVVLCLGGLAGE
                     PYDAWRIISLRGRGETLPFLRRYVPALVFPLFFIFVGLVWQALR"
BASE COUNT          806 a         1045 c         1024 g          889 t
ORIGIN      
        1 gccacctgcc actctggtac agcaacacaa acaaggcgaa ggtcaaagca ccgaggaaaa
       61 accgataaaa cagaaaaccg taaagccagg tcaggttacg cgcgatggcg gccaattgct
      121 ggcggaaaac gaagcgcggc gtcacgccct cgtaccaata ggaagggtcg taccagggtg
      181 gataactaat gccgggcggt gcgctgaatt catagacggc gggcgtggcg aagatttttc
      241 gagtgggatg aaccggcgtg ccgctgcccg tgggttcgcc ctgccaatgt gtatatcgcg
      301 tcgttccgtt aacgtaccat ccgtagttta agcgggcgct atcgccgatc gtaagtcgat
      361 gcttggcgcg tgacagcaat atgacaaagg gagtcgccaa cgtcaggaac attagcagcg
      421 cgattaaggc acgcagcaac gcgggcttga attccgctgc gcgtcgcgcc agtaggtgag
      481 caacaagaat gaaaacaaga gccagcggga acattatcgt cttagacaga aaacctacgc
      541 ccagcacaat tcccaaggct gcgaacaact gccaacgatt cggtgcggcg cggattcgca
      601 acaagagtcc acaagcgagg aagattaaag cagcgacaag cagatccgga ctggtgcgcc
      661 agacacgatt catgaacagc gacgtccaga tgaagatcgt gtaaccaaaa accagccaac
      721 tccactctgg cagggcgacg ccactaacgc gcagttcgcg gtcgcggcgc aacgcacgta
      781 gaaagtaggc gaaactgaac agcgctacgg cgtagatcag aaagttcaat aaccgcacga
      841 cggtgaattc ccagaatggt gacggcttga atagatgtag ccagatgccg agcagccagg
      901 aatagaaagg gctccacatg gcgtttactg cgacgtacca gtcgccgcgc atgtaggcgt
      961 cgccgatgtc gaggtaagag atgccatcgg gtcccatgta agcgagacca tcggcgctct
     1021 catgtcggcg tcccaatgcc tgaatgaagc ccaacgcgag cgcagctaac caactgaggc
     1081 gctcccaacg aattatcttg ctgctgggcg cactgccgcg tgtcgattcc ggcatggcaa
     1141 gctgttcggg ggaaggatca agcacgtggc gcatcttcac tctgcgtcaa atcgcttgtc
     1201 aacgagaagg agcgaagggg gaaatgggga aaaggaaaat aggggcaggg ggaaaaggaa
     1261 gaaaggggcg gtgggaaacg ggtggtttcc catttcccct tttcccctgt tccccctttt
     1321 cccgttcccc tcttatactc gccgcttaat gcagaccgac aaaaagaccc gctggcgttg
     1381 gggcgtgatc gcggcactcg caataacgct gctatcgctt ttccctcaat gctacttgtg
     1441 gtgggagcgt ggccggcagt ggaatggcag tcaggcattt ttttataccg atgagcctgc
     1501 atatgctgct tacattaatg ctctgatcga cgggcgcccc cgacgaaacg atccttacac
     1561 gggccgcgac gacagaccgg ccgcaccact acccgaatcc cttttttcga ttcagttcat
     1621 tcccgcttat ttcatagcac taccggcaag agtgttgggc ctctcaaccg cgaccgcatt
     1681 cattcttgtg acacccattg tggctttcac caccgcgcta accttatttt ggttactggc
     1741 tttgatcact gacgactctc gtgctgccgc cgcgtttgtg ccacttgtcc tttgtgccgg
     1801 attgctcgtt gccggtaatg gagtggtacg gccgtttttc ggtcagcaag caactctcgt
     1861 ctatctgcct ttcctgcgac gttatagtcc cgcagtcgtc tttccgtgtt tcctgatttt
     1921 ttttgcgttc gtttggcgaa tattgacgac cacttcgcgt cagctgcggc tcctttacgt
     1981 actcggtgcg gctgctgctt ttacagtttg tctttacggt tacttctatc tttggacggc
     2041 cgctctggcg tggcccgcgc tcgttgcatg tttatggttc acggcacgac ctgatgagtg
     2101 gcgcgctggc ttgcgaagca tcgcgttact aacggccgtc atcctgaccg tatcggtgcc
     2161 ttactcaatc ctcctcttcc ggcgtgcccc aaccatggat ttagcacagg cactagtgcg
     2221 cacgcatacc cctgatattt ggcgtgcaag cgaagcgttg gcgctactcc ttgttatagt
     2281 cgtggtggtc gcaattcttg ctcgccgcag ctcacgacaa gatccacgaa tcctgttcac
     2341 gttggctttc gcattgctgc cgttcgtgcc attcaaccaa caagtaatca cggggcgctc
     2401 gttgcagcca atgcattacg agcaatttgt agtgacctat acaaccctga ttgcattggc
     2461 cctggtaatc gtctggttgc gaagcggaag agttgagcag agtccggtcg gcgggctttt
     2521 gctgctgaca attagtttgt ttggctatgg ttggggactg ggcgaaactt ggatcgccac
     2581 tcgacggttt gcctatgtca attctttacg cgacgaggcg cgccctgttg gtctgcgatt
     2641 acgcgagttg gccaaaccac cagttatcga tcaacagaat ccacttgaag ttgttttcgc
     2701 gcccaacctg ggacggagcg acaacctgcc gaccaccgcg ccacaagctg tgctttgggc
     2761 accacacatg tttgttttct ccggtgttgc cgcagcagaa aataaggaac gcttctttca
     2821 gtttctttac ttctcgggag taggcgtcga agagttttcc cattatgaaa acgggtttgt
     2881 gaagtacgcc atttttggtt gggaacgagc caatgagaag ctaacggcca acttcagacc
     2941 gatcaccgcc gaagaaatcg ctctggaagc gaagaactac gccgactaca ttgcgaactt
     3001 cgactcaacg cgcgccaggc gaccaactct cgcgtacctt ttgctggacg caaatcagcc
     3061 tataaatttg agcaacctcg agcgttggta cacgcgcgac aacggccagc gaattggtcg
     3121 atatctcctt tatcgactaa caccgcgtac aacatgagtc aatctgtcgt gccgcgcctt
     3181 aacatcgaat ggcgattagg actcattgtc gcgatcgcaa tgagcgttct cgccctggtg
     3241 ccgcaatttc atctttgggc cgtacgttcc agcgactggc agggcacata cgtgactttt
     3301 gattttgacg aagtcgctta cgcctcgtac ttgaacgctc tgattgatgg acgtccgcgc
     3361 cgtaacgatc cctacaccgg acgagacgtt acagcccggc aaccgctgcc tgagtcctta
     3421 cactcaatcc aatttgtggc tgcttacgcc ttagcattac cggcccgagc tttgggtttg
     3481 tcagcgggcg cgacttttgt gattgtccgc gcccttgccg ccttcgcttc aactctcgct
     3541 cttttctggt tactgggttt gctcaccggt gatagccgtg ttgccgcggc cgggacaatc
     3601 gttgtgttgt gtctcggcgg tttagcgggt gaaccttacg atgcctggcg catcatctcg
     3661 ttgcgaggga ggggtgagac cttgcccttc ctgcgacgct atgtccccgc cctggtcttt
     3721 cccttgttct tcatcttcgt tggacttgtt tggcaggcat tgcg
//
LOCUS       DASUEX010000157         2289 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_31179, whole genome shotgun sequence.
ACCESSION   DASUEX010000157 DASUEX010000000
VERSION     DASUEX010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31179"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(270..>2289)
                     /locus_tag="VFV61_02615"
     CDS             complement(270..>2289)
                     /locus_tag="VFV61_02615"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX4899393.1"
                     /translation="FFQGRVDHNWNGRNEFFARHTLDDADQRLPTDFPQFPRAFRSRN
                     QFFTAENRFIQSAATVQTFRFSFSRTRIGQQVEANTSQPLQPFIPGREFIGNIDIGGV
                     PRFGPQTSVSVRLTQNVFGFEHGLVHTAGRHIIKAGGVIERYQDNMVNPTFSLGIWTF
                     ANLRNFLTNNPQSFLGLTPNGALDRYWRFTFFAAYVQDNFQVHPRLSLNAGLRYETAS
                     LPVDIHGRDSALPSLSDSAPTVGPLYKNPTRLNFSPRVGFAWDVFGDGKTSLRGGYGL
                     YFNTNNQQNLIVTVTNPPATPRIAIGFTPTSPCRPAFPTAPLSCAFANSIRPVQFDLD
                     NPYLNVYNLSVQRELWWDIVAMVAYAGARGVHLLRSNDVNIAVPTIRPDGTPFFPVGA
                     PRQNTAFSTIELKSSDGNSWYNAMIFDLRKRWSKSFSIQSSYTFSRNIDTTQASTFFS
                     DATNGTTTAFPEFPSLNYNKGLADYHAKHNWVVNFIWELPFARNVKGMGKIFLDGWQL
                     AGISNVRSGNPLTVFVQANRSRSLWQPSLGPGIGRDRASIAPGFTYESAVLGRPDQYF
                     NPAAFVLQPAGTFGNTGRGAFIGPNYRTFDLAAMKNTRFTRLLGESGNVQIRLEVFNL
                     FNRANFAPPALTAFAGAADGEAPLSTFGRIRSTVTTSRQIQLGLRLSF"
BASE COUNT          556 a          588 c          657 g          488 t
ORIGIN      
        1 gtaggcgaca ccgacacaga gtagggcgcg cataggactg ccaaggtcca cctgtccgcg
       61 gtgcagggcg ggaccataga gtccccacgc cagcgcggcg cctaacgcaa aaattatcca
      121 gagcattcaa gttctcctgt ggtgaatagt gaatagtgaa tgggcttgca caaaaagtcg
      181 caatccgcat caccgttaga ggtttcgtga aacttcaatc ttgtcttggc ctgcgaagca
      241 ggcgccagca gaatgcttag tcccctcgct caaaagctta gccgcaaacc caattgaatc
      301 tgtcttgacg ttgtaaccgt tgagcgaatg cgcccaaagg ttgagagggg cgcctcgcca
      361 tccgctgcac cggcaaaggc tgtcagcgct gggggcgcaa agtttgcccg gttaaataga
      421 ttgaatactt ccaaccggat ctgcacgtta ccagactcgc ccagcaatcg ggtgaatcga
      481 gtattcttca tcgcggccaa atcaaaagtg cggtagttgg gtccgatgaa cgcgccacgt
      541 ccagtgtttc cgaacgttcc tgccggttgc agcacaaacg ccgccggatt gaaatactga
      601 tctggcctcc caagcactgc tgactcgtag gtaaatccgg gagcaatcga cgctcggtcg
      661 cggccaattc ccggaccaag tgacggttgc cacagcgagc gtgaacggtt tgcctgaacg
      721 aaaacggtca atggattgcc gctgcgaacg ttgctgattc ctgcaagctg ccacccatcg
      781 agaaatattt ttcccattcc cttcacgttg cgcgcgaaag gcagttccca gatgaagttg
      841 accacccagt tgtgcttcgc gtgatagtct gccagaccct tgttgtagtt caagcttggg
      901 aattcgggaa atgccgtcgt cgtgccattg gtcgcatcgg aaaaaaatgt cgaggcttgc
      961 gtcgtatcga tgttgcgcga aaaagtgtac gaagactgaa tgctgaaact cttactccat
     1021 ctcttacgca gatcaaagat cattgcgttg taccaggagt tgccgtcgct gctcttgagt
     1081 tcaatggtag aaaatgcagt gttctggcga ggggctccaa cggggaaaaa aggcgtgccg
     1141 tccggtcgaa tcgtcggtac ggcgatgttc acatcattac tgcgtaacag gtgcacgcca
     1201 cgcgcaccag cgtaggcgac catggcgaca atgtcccacc acagttcgcg ctgcacgcta
     1261 aggttgtaaa cattcagata aggattatcg agatcaaact gcacgggacg gattgaattt
     1321 gcgaaggcgc atgatagcgg cgccgtgggg aaagccgggc ggcaaggcga agtcggtgta
     1381 aagccgatcg caatgcgcgg cgttgccggt gggttcgtca cggtcacgat caggttctgc
     1441 tggttgtttg tgttgaaata cagaccgtat ccgcccctca gacttgtctt gccgtcgccg
     1501 aaaacatccc aggcaaaccc cacccgaggt gaaaaattca gccgcgtggg atttttgtac
     1561 agcgggccaa ccgtcggagc actgtcgcta agagaaggca gagctgaatc gcggccgtgg
     1621 atgtccactg gcaaactggc agtttcgtag cgaagtcctg cattcaaact cagtctgggg
     1681 tgaacctgaa aattgtcctg tacgtaggcc gcaaagaaag taaagcgcca gtaacgatca
     1741 agtgcgccat tgggggttag tcccagaaaa ctttgcggat tgttcgtcag gaagtttctc
     1801 aggttggcaa aggtccagat gccaagcgag aaggttgggt tcaccatgtt gtcctggtag
     1861 cgttcgatta cgccacctgc cttgatgata tgacggcccg cggtatggac caggccatgt
     1921 tcaaaaccaa agacgttctg cgttaaccgc acgctaaccg aagtctgcgg accaaagcgt
     1981 ggcacgccgc caatatcgat gttaccgata aactcgcgtc cgggaatgaa gggttgtagt
     2041 ggttgagaag tgttggcttc gacctgctga ccaatgcggg tacgactaaa gctgaagcga
     2101 aaggtctgca ccgtagcggc gctctggatg aaacggtttt cggcggtgaa aaactggttg
     2161 cgagaacgga atgctcgcgg aaactgcggg aaatcagtgg ggagccgctg atcagcatca
     2221 tcaagtgtat ggcgcgcaaa aaactcgttg cgaccgttcc agttgtgatc cacgcggccc
     2281 tggaaaaaa
//
LOCUS       DASUEX010000158         2146 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_832752, whole genome shotgun sequence.
ACCESSION   DASUEX010000158 DASUEX010000000
VERSION     DASUEX010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_832752"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..854
                     /locus_tag="VFV61_02620"
     CDS             <1..854
                     /locus_tag="VFV61_02620"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX4899394.1"
                     /translation="SIRVPAHFCGIVGLKPTSGRIPMDGHVPIPLGTLAKAACLGPMA
                     RTVADVSLLFRVLAGIESDTAEQSHQVENSRVCWYDFNGVTPVSHDTRIAVGAAVAAL
                     EKAGLSCSEGRPAVVAVGSRLWVELFAESSAHDLREFYSGSEDLAGPVARKILDIVRA
                     QRRPEEVARTLQERDRLRHELLAMMASTPLIVAPIGATSAFPHHASKLLVDGESVGVF
                     RAFSYAQTFNVFDLPAVAVRAGSTAAGLPVGVQIVGRPFEEQRVLNAAAIVEEALGPW
                     TPGRFES"
     gene            1019..2020
                     /locus_tag="VFV61_02625"
     CDS             1019..2020
                     /locus_tag="VFV61_02625"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEX4899395.1"
                     /translation="MEPLSGILAFFFFGFIAIVLLVIAVKTIRIVPQATVMLVERLGR
                     FDKIASSGLNILIPFLDRPRAYYWTNSRPGLTSIDLREQYIDLPPQPVITRDNVTIHV
                     DSVVYWQVIDPVKAIYEMNDLVGGIVQLTITGMRAVMGEMDLDHTLSSRDAINSKLRI
                     ILDEATDKWGVKVTRVDVKNINPPEDVRITMEKQMTAERNRRALVLQAEGERQAAVTR
                     AEGEKTAAVTRAEGEKQSAILQAEGAAQARLRNAEAESEAIKRIANAIGTGDPAQYLI
                     TSRYIESLRDMTRSNNSKVIFMPVETTAMLSSIGAIKEVLSQVGEKGDDQPKPRQLP"
BASE COUNT          462 a          571 c          616 g          497 t
ORIGIN      
        1 gttcgattcg cgtgccggca cacttctgcg gcatcgtggg cttgaaaccc accagcggtc
       61 gcataccgat ggatggccac gtcccgattc cgctcggtac tcttgccaag gccgcttgcc
      121 tgggtccgat ggcacgcaca gttgcagatg tctccctctt gtttcgggtg ttggcaggta
      181 ttgagagcga tactgcggaa cagtcgcacc aagttgagaa ttcgagagtt tgctggtacg
      241 acttcaacgg tgtgacgcca gtctctcatg acacgagaat cgcagttggt gctgcggttg
      301 cggcattaga gaaagccgga ctgtcttgta gtgaaggacg accagcggta gttgcggtgg
      361 gatctcgcct atgggttgag ttgtttgccg aatcctcagc tcacgatctg cgtgagttct
      421 attctggttc agaagatctt gctgggccag tcgcgcgtaa gatattggac attgtcaggg
      481 cccagcgccg gccagaagag gtggcgagaa cgctacaaga gcgagaccga ttgcgacacg
      541 aactattggc gatgatggcg tcgacgcctc tgatcgtggc acccattggc gccaccagcg
      601 cttttccgca tcacgctagc aagctgttag tcgacggcga atcggtcggc gtgttccggg
      661 cgttcagtta tgcgcaaact tttaatgtat tcgatctgcc tgcagtggca gtgcgggctg
      721 ggagcacggc ggctggattg ccggttggcg tgcagatagt cggccggcca tttgaagagc
      781 aaagagttct gaacgcggct gcgatcgtcg aagaggcgct tggcccttgg acgccgggac
      841 gtttcgaatc ttaggattgg atcaatcggc aaatcgccaa tcggcattcc caggcgcgcc
      901 cgttaccgcg tcgctagact tggcgctgtc tttcttttcc cggtttcgat ccgatatagt
      961 gccagtcaga gttcgcgttc acgacgtcaa cgaattgttt tgttagagga gtcaaatcat
     1021 ggagccccta tccggtattc tcgccttctt tttcttcggc tttatcgcga ttgtcctgct
     1081 tgtcattgcg gtgaagacga ttcgcatcgt tcctcaggca accgtgatgt tggtagaacg
     1141 gctaggtcgc ttcgataaga ttgccagtag cggcttaaat attctgattc cttttctcga
     1201 tcgaccccgt gcctactact ggaccaacag tcgtcccggt ttgacttcaa ttgatttacg
     1261 cgagcagtac atcgatctac cgccacaacc ggtgatcacg cgcgacaacg tgaccattca
     1321 tgttgactcc gtcgtttact ggcaggtgat cgatccggtc aaggcaatct atgagatgaa
     1381 cgacctcgtc ggcggtatcg tgcaattgac gatcaccggc atgcgcgcgg taatgggtga
     1441 aatggatctt gatcacactc tttcctccag ggatgcaatc aattcgaaac tacgaatcat
     1501 tctcgacgaa gcgactgata aatggggagt caaggtgacg cgcgtggacg tcaagaacat
     1561 caatcccccg gaagacgtgc gcatcacgat ggaaaaacag atgactgctg agcgcaaccg
     1621 gcgcgccctc gtattacagg cagagggcga acgtcaggct gcggtcaccc gcgctgaagg
     1681 tgagaagact gcggctgtta ctcgtgctga gggcgaaaag cagtcggcca ttctgcaggc
     1741 ggaaggtgcg gcccaggccc gcttgcgcaa cgccgaagct gagtcagagg ccattaagcg
     1801 aatcgccaat gcgatcggca ctggtgaccc ggcacagtat ctaatcacct cgcgctacat
     1861 cgaaagtctc cgtgacatga cgcgcagtaa caactccaaa gtgatcttca tgccggttga
     1921 gaccacagcc atgctctcca gcatcggggc cattaaggaa gtcttatcgc aggtgggtga
     1981 aaaaggcgat gaccaaccga aacccaggca actaccttaa cgtttcagca cgtgcctcga
     2041 gattgtcgtg tatctcgagc cggcgctgcc gagctcactg cgaataagca acacttcgtt
     2101 aacgctgatt tcctctggta caaatttgag ctcccgatgg gcgcgt
//
LOCUS       DASUEX010000159         2883 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_487730, whole genome shotgun sequence.
ACCESSION   DASUEX010000159 DASUEX010000000
VERSION     DASUEX010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2883
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487730"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..889
                     /locus_tag="VFV61_02630"
     CDS             <1..889
                     /locus_tag="VFV61_02630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX4899396.1"
                     /translation="LRDHEDSDVTAEEPEQSQTRLRELRLAIVGRPNVGKSSLLNSLL
                     GRERVIVSPLAGTTRDAIDTVLETPEQTFRIIDTAGIRRKGRTNEMAEKLSVVMARKS
                     LERADVAVVLIDAEEGPTALDANIAGYAFDAGCSIIIAVNKWDAIADKETNTALEFER
                     RVREKMKFLDWAPVVTISALKGQRVDRILPLTIRANEARNRRISTSQLNAFFERAIAQ
                     PRGGATPAPVRGGVSRLHVQYLTQIAVRPPTFVVFTAGGKPGLHFSYERHLQNRLREE
                     FDFFATPLRIVERHKKRKK"
     gene            1093..2271
                     /gene="tgt"
                     /locus_tag="VFV61_02635"
     CDS             1093..2271
                     /gene="tgt"
                     /locus_tag="VFV61_02635"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEX4899397.1"
                     /translation="MKPPISFEVEATSGAARTGTLKTRRGLIETPVFMPVGTAGTVKG
                     TRFEALEANDLDARIILGNTYHLWLRPGIETIQACGGLHRFIGWQRALLTDSGGFQVW
                     SLGKLRRINEAGTEFRSHVDGSLRFLSPEVSMEVQAALGSDIVMVFDECAPGQAPAGE
                     ARQSMELTARWAKRSREKFDELQREGKDLGRRDGETELSGAQALFGIVQGAAHHELRR
                     ESLDRTREIGFDGYAIGGLSVGEEKSVMWNIIDGIVPLMPVGQPRYLMGVGTPEDLIE
                     AVARGVDMFDCVLPTRNGRTGQAFTSRGKLNVKNAQWATDTRPLDEACSCSVCGRHSR
                     AYLRHLYTAGEMLAGVLITHHNLAFFLDTLRHVRQSIRSGDFPKFRREFTERLSANGE
                     "
     gene            2353..2655
                     /gene="yajC"
                     /locus_tag="VFV61_02640"
     CDS             2353..2655
                     /gene="yajC"
                     /locus_tag="VFV61_02640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEX4899398.1"
                     /translation="MTNFLMLFQGGFGALSGLLPMVLIIAVFYVLLIRPQQKRQRDLQ
                     ATIAQLKAGDRIVTTGGVIGTITSVKDTSFLIRSAEKSILEIARSAVAGVEEEEKK"
     gene            2684..>2883
                     /locus_tag="VFV61_02645"
     CDS             2684..>2883
                     /locus_tag="VFV61_02645"
                     /inference="COORDINATES: protein motif:HMM:NF019187.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899399.1"
                     /translation="MKKQNLLQRIIIISVVTLVSLYLVIGPRRKPTLNDFTWSGIKTT
                     VANNIHLGLDLKGGSHLVMKVKT"
BASE COUNT          653 a          743 c          855 g          632 t
ORIGIN      
        1 tctcagagat cacgaggact ccgacgtcac ggctgaggag ccggagcagt cacaaaccag
       61 actgcgtgag ttgcgcctgg ccatcgtcgg ccgtccgaat gtgggcaagt cttcattgct
      121 caacagtctg ctggggcgtg agcgcgtgat tgtatcgcca ttagcaggga caactcgtga
      181 tgcgattgac acggtgctgg aaacgcctga acaaacattt cgcatcatcg acaccgccgg
      241 gatccgtcgc aagggcagga caaatgagat ggccgaaaaa ctttcggtgg tgatggcgcg
      301 caagagtctc gagcgggccg acgttgctgt tgtgttaatc gatgccgagg aagggcccac
      361 cgcgctggat gccaacattg ccggatatgc tttcgatgcc ggctgctcga tcattattgc
      421 tgtcaataag tgggatgcca tcgcagacaa ggaaacgaat acggcgcttg aattcgagcg
      481 ccgggtcagg gagaaaatga agtttcttga ctgggcccca gtggtaacga tctcagcact
      541 caaggggcag cgagtcgaca ggatcctgcc actcacgatt cgtgccaatg aagctcgcaa
      601 ccggaggata tcaacgtcgc agttaaatgc gttctttgaa cgcgcgattg cccagccgcg
      661 tggaggagcg acgcctgcac cagttcgtgg cggcgtttcc cggttacatg tgcagtacct
      721 gacgcagatt gcggtgcggc cccccacgtt tgtcgtgttc accgcaggag gtaagccagg
      781 cttgcacttt tcgtacgaac gtcatctgca aaaccggttg cgggaggaat ttgatttctt
      841 tgctacgcct ctccggatag ttgaacgaca taagaagcgt aagaagtaga cagtagacag
      901 tagacagtaa acggtagacg gtagtcagtg gtccgaagtt agttgtcagt tatcagtttt
      961 tttcaattgt cgggctccgg cctcggctaa cgagtgatga ctagctacag tctactgact
     1021 actgcccgct aacgactgcc cactgacaac tgacaacgga caactgccca ctgactactg
     1081 acaatccttc gaatgaaacc tccgatcagt tttgaagttg aagctacgag tggcgcggcc
     1141 agaaccggaa ccttaaagac tcgccgcggc cttattgaga cgccggtctt tatgcctgtg
     1201 ggtaccgcgg gcacggtcaa gggaacgcgt ttcgaagcgc tcgaagccaa cgatcttgac
     1261 gcgcgcatca ttctgggcaa cacctaccat ctttggttgc ggccaggcat cgagacgatt
     1321 caggcttgcg ggggactgca tcgctttatt ggatggcagc gcgcgctgct tactgactcg
     1381 ggaggctttc aggtctggag cttggggaag ttgcgacgga ttaacgaagc tgggacagag
     1441 tttcgttcgc acgtcgatgg cagtctacgt tttctctcgc cagaagtctc gatggaagtt
     1501 caggcggcgt tgggctctga catagtgatg gtctttgacg aatgtgctcc cggtcaggct
     1561 cctgccggtg aagcgcgtca gagcatggaa ttgaccgctc gttgggcgaa gcgctcgcgg
     1621 gagaagtttg acgaactaca gcgagagggg aaggacctgg gacggcgcga tggcgaaact
     1681 gaattgagcg gggcgcaggc attatttgga atcgttcagg gagctgcgca tcacgaactg
     1741 cggcgggaaa gtctcgaccg cacccgcgag attgggttcg atggttacgc gatcggtggc
     1801 ctgagcgtcg gcgaagaaaa gtctgtaatg tggaacatta tcgatgggat cgtcccgctg
     1861 atgccggttg ggcagccgcg ctatctgatg ggcgtcggta cacccgaaga cctcattgag
     1921 gcagtggcgc ggggcgtgga catgttcgac tgcgtgctgc cgacgcgcaa tggccgaacc
     1981 ggccaggcat tcacttcgcg ggggaaattg aacgtcaaga acgcgcaatg ggcgaccgat
     2041 acgcggcccc tggacgaggc ctgtagctgt tcagtttgtg gccggcattc gcgcgcttac
     2101 ctgcggcatc tatacaccgc cggtgagatg cttgcgggcg ttttgatcac tcatcacaat
     2161 ctggccttct tccttgacac tctgcggcac gtgaggcaat ctatccggtc tggcgacttc
     2221 cctaaatttc gacgagagtt cacagagcgt ctaagcgcga atggggaata acataagccg
     2281 cgcttggaat caggctcaat gcggtctaaa cgattgaaga ttccgtcgtt tactcacgaa
     2341 taaaaacacc agatgacaaa ctttctaatg ttgtttcagg gcggcttcgg agcgcttagt
     2401 ggtctgctcc cgatggtgct gatcattgcc gtcttttatg tattattgat tcgtccgcaa
     2461 cagaagcggc aacgtgactt gcaagccacc attgcgcagc tcaaggccgg agaccgcatc
     2521 gttacgacgg gcggcgtgat tggcacgatc acgtccgtta aagacaccag ctttttgatt
     2581 agaagcgccg agaagtcgat cctggaaatt gcgcgttccg ccgtggcggg cgtcgaagaa
     2641 gaggaaaaga agtaaccgat ccaagggcct aaacgttctt gttatgaaga aacagaacct
     2701 gctgcaacgc atcatcatca tctcagtcgt taccctcgta agcctgtatc tcgttatcgg
     2761 ccctcgccgt aaaccgacgc tcaatgattt cacgtggtcc ggcatcaaaa ctacggtcgc
     2821 caataacatt cacctcggtc tcgatctcaa aggcggatca cacctggtga tgaaggtgaa
     2881 gac
//
LOCUS       DASUEX010000160         2655 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_832929, whole genome shotgun sequence.
ACCESSION   DASUEX010000160 DASUEX010000000
VERSION     DASUEX010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_832929"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..337
                     /locus_tag="VFV61_02650"
     CDS             77..337
                     /locus_tag="VFV61_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899400.1"
                     /translation="MPKGDRGAYTDKQKRQAEHIEDSYEERGTSKKEAERRAWATVNK
                     ESGGGKKSGSGRAKKGNKSASKKGGKKAAKKSGSKKSSAKKR"
     gene            380..568
                     /locus_tag="VFV61_02655"
     CDS             380..568
                     /locus_tag="VFV61_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899401.1"
                     /translation="MGDNGGSGSTGVVAILVIFVIVVVAALFVFGGRIFSGNKKVDVN
                     ISAPSAPASSQPAPAPSR"
     gene            636..1043
                     /locus_tag="VFV61_02660"
     CDS             636..1043
                     /locus_tag="VFV61_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899402.1"
                     /translation="MKELLQEKQGRLYAAAAMRSRGEVFDVTRTSLDQSYEGWLLAMK
                     ATEELNMSAQAALISNDLRRPHELKQKPALQLCQPIEARQIDPGPHSSKLTTQEYVQV
                     MMATIREGRQHARWPREVIEHFLAAEVIVSSEQ"
     gene            complement(1180..>2655)
                     /locus_tag="VFV61_02665"
     CDS             complement(1180..>2655)
                     /locus_tag="VFV61_02665"
                     /inference="COORDINATES: protein motif:HMM:NF013560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M2 family metallopeptidase"
                     /protein_id="HEX4899403.1"
                     /translation="VLPAPDNPAEREEITKLAASLEGDYGKGKYCPDGEKGKCLSLGD
                     MEEILANSRNPEELKRIWLGWHQVSPPYRKDYERFVELSNKGAREMGFKDTGAMWREK
                     YDMEPEAFAAEMERLWQQVKPLYDSLYTYTRRKLSEKYGKEVVPPDGPIPAHLLGNMW
                     AQTWGNVYPIIAPQGSGDRGYDLTATLKSRNTDAKQMVRYGESFFTSLGFEQLPATFW
                     ERSLFTKPSDREVVCHASAWDVDFEKDVRLKMCIQINEEDFSVIHHELGHNYYQMAYA
                     GQPFLYRDSANDAFHEAIGDTIALSVTPPYLKQIGLIDKVPNQSADIGFLLNRALDKV
                     AFLPFGYLVDQWRWKVFSGEVGPGDYNKAWWDLRTKYQGVAPPVARSEQDFDAGAKYH
                     VPANVPYSRYFLAAILQFQFHRALCREAGFNGPLYQCSIYGNKQAGQKLNQMLALGLS
                     KPWPEALKTMTGEDKMDATAIIDYFAPLKQWLDAENQKAAQ"
BASE COUNT          630 a          695 c          743 g          587 t
ORIGIN      
        1 gatgaagttc gtcggctcag tgtttgcaga gcacgaggtc tggccagtta acaaaaatca
       61 acacggggtg tgaacaatgc cgaagggaga cagaggagct tacaccgaca agcagaagcg
      121 tcaggccgaa cacattgaag actcttacga agagcgcggg acgtctaaga aagaagcgga
      181 gcgacgcgcc tgggccacag tgaataagga atctggtggt ggcaaaaaga gcggcagcgg
      241 ccgcgccaag aaagggaaca agagtgcttc taagaagggc ggaaagaaag ccgccaaaaa
      301 gagcggttct aagaagagca gtgcgaaaaa gcgctgatgt tgaaacaacg gcgggataaa
      361 tcaatggagg attcagataa tgggtgacaa cggtggcagt ggcagcacag gtgtagtggc
      421 aattctggtg atctttgtga tcgtcgtagt ggcggcgctc tttgtattcg gcgggcgcat
      481 tttcagcgga aacaagaagg ttgatgtaaa catttccgct ccgtcagcac cggcatcgtc
      541 tcaaccagcg ccggcgcctt cgcgataaat cccgcaagtt cttgcgcgag agctacttgg
      601 aaataagcac gtattagtag ttcttagtaa ttaccgtgaa agaacttctg caggagaagc
      661 aggggcggct gtatgccgcc gccgcgatgc gttccagagg cgaggttttc gacgtcactc
      721 ggacttcact tgaccagtca tacgaaggct ggctgctcgc aatgaaagcg actgaggagc
      781 taaatatgtc ggcacaggca gcattaatca gcaacgacct ccgacgcccc cacgaactga
      841 aacaaaagcc agccttgcaa ctctgtcagc caatcgaggc ccggcaaatt gacccgggcc
      901 cgcattcgag caaattgacg acgcaggaat atgtgcaggt aatgatggcc actatccgcg
      961 aaggccgcca acatgcccgt tggcccaggg aagttattga gcacttcctt gcggcggaag
     1021 tgatcgtcag cagcgagcaa taagtactag gtacattgga taacgctaag ccgctgtgac
     1081 gcgaaggcgc aaagaagaag gatatgggtt ggcgttgcag atgattcgtt ctttgcgact
     1141 cggtgccttg gcgtcagagt cgcaattttt cctgcctcct cactgtgcgg ccttctgatt
     1201 ctcagcatct agccactgct tcagcggcgc gaagtaatca ataatcgctg tcgcatccat
     1261 cttgtcttca ccagtcattg tcttgagggc ctcgggccac ggcttgctca agcccagcgc
     1321 caacatctga ttgagttttt gtcccgcctg cttgttgcca taaatcgaac attgatagag
     1381 cggcccgttg aagcccgcct cgcgacagag cgctcgatga aattgaaact gtaggatcgc
     1441 cgcgaggaag taacgcgaat atggaacatt cgccgggacg tgatatttcg cgccggcgtc
     1501 aaaatcctgt tccgagcgtg ccactggtgg cgcgacaccc tggtacttgg tccgtaagtc
     1561 ccaccaggct ttgttgtaat cgcccggacc aacttcaccc gaaaaaactt tccagcgcca
     1621 ctgatccacc aggtaaccaa aaggcaggaa cgccaccttg tccagggctc gattcaacag
     1681 gaagccaatg tcggcacttt gattcggcac cttatcaatc aaaccgatct gtttgagata
     1741 gggaggagtt actgatagcg cgattgtgtc gccgatcgct tcatgaaagg catcgttggc
     1801 gctgtcgcga tagagaaagg gctgtcccgc atatgccatc tggtagtagt tgtgtcccag
     1861 ttcatgatgg atgaccgaga agtcttcctc gttgatctga atgcacatct tcaggcgcac
     1921 gtctttctcg aagtcgacgt cccaggcgct cgcatgacag acgacctcac gatcagaagg
     1981 tttagtgaag agcgaacgct cccagaaggt tgccggaagt tgttcaaagc ccagcgaggt
     2041 gaaaaacgat tcgccgtaac gaaccatctg tttcgcatct gtattgcgac tcttcagcgt
     2101 ggctgttaag tcgtagccgc gatcgccgct gccttgcggg gcgatgatgg gatagacatt
     2161 tccccacgtc tgcgcccaca tgttgccgag caagtgagcc ggtatcggac catcgggtgg
     2221 cacgacttcc ttaccgtatt tttctgagag cttgcggcgc gtgtaggtgt aaagcgaatc
     2281 gtagagtggt ttcacctgct gccatagtcg ctccatctca gccgcgaaag cttcgggctc
     2341 catgtcgtac ttctcgcgcc acatggctcc cgtgtccttg aaacccattt cgcgcgcacc
     2401 tttattactg agttctacaa agcgctcgta gtctttgcgg tagggaggtg aaacctgatg
     2461 ccagccgagc cagatgcgtt tgagctcttc cggattccgg ctgttagcaa gaatctcttc
     2521 catgtcgccg agactcaggc atttcccttt ttcgccgtcg gggcagtact tccctttgcc
     2581 gtagtcgcct tcgagggacg ccgctaactt cgtgatttcc tcgcgttccg cagggttatc
     2641 tggagccggc aatac
//
LOCUS       DASUEX010000161         3338 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_487784, whole genome shotgun sequence.
ACCESSION   DASUEX010000161 DASUEX010000000
VERSION     DASUEX010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3338
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487784"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(276..1244)
                     /locus_tag="VFV61_02670"
     CDS             complement(276..1244)
                     /locus_tag="VFV61_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899404.1"
                     /translation="MRRLFDPNIIIPSTNQFIFLWIKMQLVISLPRTFNFKRTVISHG
                     WSGLLPFSLDHKQWVLTRALEIPQKAPVTVSLSATRGELRIDSSRRLSKDASEAVKRD
                     VRHMLRLDDDMRSFYQSVNQVPGFEWIAQTGAGRMLRSPTVFEDLVKMICTTNCSWAL
                     TEKMVTGLVRNLGRETNEGRRTFPAPEAMALMPLKFYVNEVRAGYRAPYLKELAERVA
                     SGSLQVEQWLQTELSTAELVKEMRGVKGVGPYAAENLLKLLGRYDGLALDSWTRAKFY
                     KVRNNGRKASDKKIERYYSRFKEWRGLALWCDMTRDWLDAEGNSAH"
     gene            complement(1654..2868)
                     /locus_tag="VFV61_02675"
     CDS             complement(1654..2868)
                     /locus_tag="VFV61_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899405.1"
                     /translation="MKRWQKALLITVVLLLISQIPFAYRRFKLGKLHAAILTVQSQRQ
                     PTPDREALQEFRGVVHVHSFLGGHSSGTFQEIIAAANSNQLNFVLMTEHPAREFSTAE
                     MTLKGEHGGVLFINGNEVSASNGDRLLLFPGEAQAASDNPQSTQEILSQRSTGISFVA
                     YPEDFKSWDAHGFNGVEVYNLYTDARKINALKMFFDGLWSYNSYPDLLFATFYQRPAA
                     NLKSWDEVSKSKNLKLVAIAGNDAHANVGVALTDSSGNTLLGLKLDPYERSFRIVRLH
                     VLAAPLHGATLNEATLLDAISSGHCFIGFDVFGDSSGFRFTATTGREVKQMGDEITLV
                     DRVQMNAKVPVPARIVLLKDGVIIEDKAGFRELEYLVTEKGTYRVEVYLPQLPAPVSG
                     QPWIISNPIYVR"
     gene            complement(2890..3180)
                     /locus_tag="VFV61_02680"
     CDS             complement(2890..3180)
                     /locus_tag="VFV61_02680"
                     /inference="COORDINATES: protein motif:HMM:NF019491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HEX4899406.1"
                     /translation="MLTHIVIWKYKADVEPEVREEHLRLLRNLKSAIIEVQSLRVGSD
                     IVHLPRSYDTGLVASFRDRAGLDAYTIHPEHIKVAEFGRSISEQVASVDFED"
BASE COUNT          783 a          910 c          846 g          799 t
ORIGIN      
        1 acgctcgacc tgtcatataa gcgcgtcttt aacaccccga atggcgaagt cactgcaacg
       61 accactgaga agctatcact cagcgcggac ggtaaggtct tgaccaacgt tcgtcacagc
      121 gagagtccac gtggtccaca agattcgacg ctcgtcttta acaagaaagg ttaggaagtc
      181 tacgggcggt ggtagcaatt tggcttcgga ggaacgccag gatcaagagc tgtggcgggc
      241 ctccgcagct tcaaatgggt tatgaggtag gattttcagt gtgcagagtt gccctcggcg
      301 tcgagccaat cccgcgtcat gtcacaccac aaagcgagcc ctcgccactc tttgaaacgc
      361 gaatagtacc gctcaatctt cttgtcgctc gccttgcggc cattattgcg cactttgtaa
      421 aactttgccc tggtccagga atcgagcgcc agtccatcgt aacggcccaa caacttcaac
      481 aagttttcgg cggcgtatgg accaactcct ttcacgccgc gcatctcttt gaccagttcc
      541 gcagtagata actcagtctg taaccactgc tcgacctgca aagaacccga tgcgacccgt
      601 tctgccaact ccttgagata aggtgcgcga tagccggcac ggacctcatt cacataaaac
      661 ttcaatggca ttaacgccat cgcttcgggc gcaggaaacg tccgtcgtcc ctcgtttgtc
      721 tcccgcccca ggttgcgaac gagtcctgtc accatctttt ccgttaacgc ccaggagcag
      781 ttagtggtgc agatcatctt caccagatct tcaaagactg tcggagatcg caacattcgg
      841 cccgctcccg tctgggcaat ccattcgaaa cctgggacct gattcacgct ctgataaaaa
      901 ctccgcatgt cgtcatccag gcggagcata tggcgcacat ctcgcttcac agcttcactt
      961 gcgtctttac tgagccgccg ggacgaatca attcgcaact cacctcgggt agctgagagc
     1021 gagacggtaa cgggcgcttt ttgaggaatt tcgagcgcgc gagtgagcac ccattgtttg
     1081 tggtcaagcg aaaatggtag taagccactc cagccgtgac tgatgaccgt gcgtttaaag
     1141 ttaaaggttc ggggtagaga gataacaagc tgcatcttga tccatagaaa gatgaattgg
     1201 tttgttgaag gaatgattat gttcggatcg aagagtcggc gcaaaccgtg gtttctttcc
     1261 gtcgcttacc tctatcctgt attgtctcct caaggagctt ttggagcctt tgtatgcttc
     1321 agattcggtc aacgtgattc ttgacagagc ctaccctcag gactagacta acttcacttg
     1381 aaaacagtgt gtggaaggct gttctcgggt ttccctaata ctcgggaaga cctgagcgtg
     1441 gagtcgatca ctctggctct ctacagcata taacggtgaa cgtacgcacc gcttccaaat
     1501 aaactagccg gagctttccg gcccccgttc gtcgccccat tgggcagttg cagggcagcc
     1561 catggggcga tgtcatgtct ggcctccgaa aactcgttgc cttcctcaaa tttcgtgtaa
     1621 tttctgggat cttctgaggt atggaaggca cttttatcta acataaatcg gattcgagat
     1681 tatccaaggc tgcccactca caggggccgg taactgaggc agatagacct caacccgata
     1741 agtgcccttt tccgtcacca gatactcaag ttccctaaaa cccgctttat cctcgattat
     1801 cacgccgtcc ttgagcagca caatcctcgc gggaacgggc accttcgcat tcatctgcac
     1861 tcgatcgacc agggttatct cgtcccccat ctgtttcact tctctccccg ttgttgctgt
     1921 gaaacgaaag cccgaactgt ctccgaaaac atcaaagccg atgaagcaat gaccagacga
     1981 gattgcatcc aagagcgtgg cttcattcaa tgtagcgcca tgcaacgggg cagcaaggac
     2041 atgcaggcga acaattcgaa aactgcgctc ataaggatcc agtttcaggc ccagcaaggt
     2101 attgcccgat gaatccgtca aggcaacccc gacgttggca tgagcgtcat taccggcaat
     2161 tgcgaccagt ttcaggtttt tactcttgct tacttcatcc cagcttttga gatttgccgc
     2221 aggacgctga tagaaggttg caaacagaag gtcgggataa ctgttgtaag accagagccc
     2281 atcaaaaaac atcttcaggg cattgatctt tcgtgcgtcg gtgtagaggt tgtaaacctc
     2341 gaccccattg aatccgtgcg cgtcccagct cttgaaatcc tctggatagg cgacgaagga
     2401 gatgccggtc gaacgttgtg acaagatttc ctgggttgac tgcggattgt ctgaagccgc
     2461 ctgcgcctcg ccggggaaaa gcagcaggcg gtcgccgttg gacgcgctca cctcattccc
     2521 gttaatgaac aagacaccgc catgttcccc cttcagggtc atttccgccg tgctgaattc
     2581 gcgcgctgga tgctctgtca ttagcacaaa attcaactga ttcgaattgg cagccgctat
     2641 gatctcctga aaggttccac tgctgtgccc gcccaggaag gagtggacat gaacaactcc
     2701 gcggaattcc tggagtgcct cgcggtcggg cgtgggttga cgttgggatt gaacggtaag
     2761 aatagcggcg tggagcttgc ccagcttgaa acgacgatac gcaaaaggga tctgcgatat
     2821 caacaggaga actactgtga ttagtaaagc cttctgccag cgtttcatca agatttgctg
     2881 ccgacgttct cagtcctcga aatccactga ggccacctgt tcgctgatgc ttcggccaaa
     2941 ctcggcgact ttgatatgtt ccggatggat ggtgtaagcg tcgagcccgg cgcgatcacg
     3001 aaacgatgct accagtccgg tgtcgtagga acgtggcaga tgcactatgt cagatccgac
     3061 ccttagactc tggacctcga taatggccga cttgaggttg cgcaacaggc gcaggtgttc
     3121 ttcccggact tcgggttcca catcagcttt atatttccag attacgatat gtgtaagcat
     3181 gatcattaaa aggaggaagg atgaaggcgg aaggatgaaa gtcaaccaca agagttgtga
     3241 gctaacattt aggttctcgc tatgtcagcg catccgcctt ccgtcttccg tcccttcgca
     3301 ccatctccgc gttacgtcgt gccggtagtc aaatagcc
//
LOCUS       DASUEX010000162         2704 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_718449, whole genome shotgun sequence.
ACCESSION   DASUEX010000162 DASUEX010000000
VERSION     DASUEX010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_718449"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            252..1619
                     /locus_tag="VFV61_02685"
     CDS             252..1619
                     /locus_tag="VFV61_02685"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX4899407.1"
                     /translation="MPDILLVEDKESLRRVLRLTLENAGYSVTEAADARIALNEIASA
                     PHRIVLTDLRMPNGSGLDVLHAARAADSDVPVIVMTAFGSIDEAVQAMKDGAQDFLQK
                     PVDSNHLLLLVERALEQARLRTENILLREEWSRRYGFPRILGESEAIKRTVAETQRVA
                     QTEANVLLLGESGTGKELFARALHHLSHRREKPFVAINCAAIPETLIENELFGHERGA
                     FTGASDRRLGKFELAAGGTVFLDEIGELPLAVQGKLLRAIEEKTVDRIGGRAPLPVDV
                     RVVAATNKDLKGAVEKGEFRGDLFFRLAVFPIEIPPLRQRDNDVLLLARDFAAQLGKE
                     LRGKAATLSEHSLRALQAHQWPGNVRELENAIERACILTDTMVLEPKDLGLAERAGED
                     AEQLQSLDLSGTLFEVTQRAIRSVERRKIVAALAANANNKTRTAEDLGVSYKTLLTKL
                     KDYDL"
     gene            1626..>2704
                     /gene="murJ"
                     /locus_tag="VFV61_02690"
     CDS             1626..>2704
                     /gene="murJ"
                     /locus_tag="VFV61_02690"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEX4899408.1"
                     /translation="MSDSPERQSPAPSSEAESIGVHHDAGAIAGETLEPATAATSAPD
                     TDRVTKGQPGTGRSAFFVGTGILISRIVGLIRQRIFAHYFGTSAAGDAFSAAFRIPNF
                     LQNIFGEGALSASFIPVYAKLLAADDEAEASRVANAVLGLLALVTSLIVLIGVLTTPY
                     LIDLIAPGFSGETRALTIRLVKILFPGAGLLVLSAWCLGVLNSHHRFFISYTAPVVWN
                     LAIIGALVSFGNKVGQFPLAEFTAWGSVIGSALQFGIQLPTVFKLVQRLRPVIDVANA
                     QVRTVVRNFFPVFMSRGVIQISAFVDAILASFLPTGAVVALTYAQSLYTLPVSLFGMS
                     VSAAELPAMSRAIGSDEEVAHVLRLR"
BASE COUNT          550 a          809 c          735 g          610 t
ORIGIN      
        1 tgaagtcggt accactacac ggtagcgggt ggaccggtca catcccgagc gcaatcgtga
       61 atttccgatt gtttttttag ctacgagcaa accgccaatc gccagtcacc aatcggcaat
      121 ccctgatacc gaccctccgc accactagct caaaagaaca tccttgacac gggtctgctt
      181 agcgggaatg atgaatcgac accggcgcaa atcgcgccgg tcgttctagc tggcccctga
      241 ccctgtcgcg catgcctgac atacttctcg ttgaagacaa agaaagccta cgccgcgttt
      301 tgcgtttgac tctcgaaaac gccggctatt ccgtcacgga agccgccgac gcccgcatcg
      361 cgcttaatga aatcgccagc gctcctcatc gcatcgtgct cactgatttg cgcatgccga
      421 acggctctgg tctggatgtc ctacatgccg cccgcgccgc cgacagcgat gtgcccgtaa
      481 tcgtgatgac tgcctttggc tccatcgatg aagccgttca ggcgatgaaa gatggggccc
      541 aggacttcct gcaaaaaccg gtcgactcca atcatcttct acttttggtt gaacgcgccc
      601 tggaacaggc acgcctgcgc accgaaaaca ttctcttgcg agaagaatgg tcgcgccgct
      661 acggctttcc gcgcattctc ggagagtcgg aagcaatcaa acgcacagtc gccgaaacgc
      721 agcgtgtcgc tcaaactgag gccaacgttc tgctgctggg tgaatccggc accggcaagg
      781 aactgtttgc gcgcgcgctt catcacctct ctcaccgtcg cgaaaagcct tttgtggcca
      841 tcaactgcgc agccattccc gagacactca ttgaaaacga gttgtttggc cacgagcgtg
      901 gcgcctttac cggagccagc gatcgccggc tcgggaagtt tgaactggcc gccgggggca
      961 ccgtctttct ggatgagatc ggcgagttgc cgctggccgt tcagggcaaa ctgctgcgcg
     1021 ctatcgaaga gaagaccgtg gatcgcatcg gcggacgagc gcctttgccc gtcgacgtgc
     1081 gtgttgtagc cgcaaccaac aaagatctga agggcgccgt ggaaaagggc gagtttcgtg
     1141 gcgacctctt cttccggctc gcggtctttc cgatcgagat tccacctttg cgccagcgcg
     1201 ataatgatgt tttgctgctg gcgcgagact ttgctgctca actcggtaaa gagttgcgcg
     1261 gaaaagcggc aaccctcagc gagcattcgc tgcgtgcctt gcaagcacac caatggccgg
     1321 gaaatgtacg cgaacttgaa aacgcaatcg aacgcgcttg catcctgact gatacgatgg
     1381 tgttggaacc gaaagacctg ggtctggctg aacgcgccgg cgaggatgcg gagcagttgc
     1441 aatcactcga tttgagcggc acgcttttcg aagtaaccca acgagcaatt cgatcagtcg
     1501 aacgccggaa gatagtcgca gcgttagcag ccaacgccaa taataaaacc cgcaccgcgg
     1561 aggatctggg tgtcagttac aagacactct taacaaaatt gaaggattac gatctctgat
     1621 cagagatgag cgattctcca gaacgtcagt cacctgcccc ttcttccgaa gctgaatcga
     1681 tcggtgttca ccacgacgcg ggagctatcg ccggcgagac tttggaaccg gcaacggccg
     1741 ctacttcggc cccggacacg gatcgggtaa caaaaggaca accaggaacc ggacggtccg
     1801 cattcttcgt tggcaccggc atcctcatta gccgcatcgt cggactgatt cgccagcgta
     1861 tcttcgccca ctacttcggc acctcagccg ccggcgatgc tttcagtgcc gcctttcgca
     1921 tccctaactt tttacaaaat atcttcggcg agggcgcact ttcagcttca ttcattccgg
     1981 tttacgcgaa actgctggct gctgacgatg aagccgaagc ctcgcgtgtc gcgaacgcgg
     2041 tgctcgggtt gctcgcttta gttacctctt tgatcgtcct gattggagtt ctgactactc
     2101 catatctgat cgacctcatt gctcccggat tctcggggga gacgcgcgca ctaacgatcc
     2161 ggctcgtcaa gattctgttt cccggtgcgg gcctgctcgt gctttcggcg tggtgtctcg
     2221 gcgtgctcaa cagccaccac cgctttttta tttcttacac ggcgccggtt gtttggaatc
     2281 tcgccatcat cggcgccctt gtcagttttg gaaataaagt cgggcagttt ccgctcgcgg
     2341 agtttaccgc ctggggctca gtcatcggca gcgccttaca gtttggaatt caacttccca
     2401 ccgttttcaa gctagtgcaa cggctgcgtc cggtgattga tgtcgcaaat gctcaggtgc
     2461 ggacggtggt gagaaacttc tttcccgttt tcatgagtcg gggcgtgatt cagattagcg
     2521 cctttgtcga tgcgattctt gccagtttct taccgactgg cgccgtcgtc gcactcacat
     2581 acgcgcagag cctctacacc ttgccggtca gccttttcgg gatgtccgtc tccgctgcgg
     2641 agctgccggc catgtcccgg gccatcggtt ccgatgaaga agttgcacac gtcctccggc
     2701 tgcg
//
LOCUS       DASUEX010000163         4131 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1061905, whole genome shotgun sequence.
ACCESSION   DASUEX010000163 DASUEX010000000
VERSION     DASUEX010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4131
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1061905"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..701
                     /locus_tag="VFV61_02695"
     CDS             <1..701
                     /locus_tag="VFV61_02695"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEX4899409.1"
                     /translation="TLARPIQATLPSRIQQRFHLWLDGFDPPSPEVSWWKKDYDEALN
                     RTPELKEIAQQSPELQKTVNVDIWFDKLAFQPAQAVFGITSGKTTGRGLGLGFPEVIP
                     IADSDYVYAAIAEETGLFGGAIIILAVIIFVAAGVRTSLDARDMFTKLCAAGLTAFVG
                     FQALVNIGGILRALPMTGITLPFVSHGGFSLITSYAMLGMLMAFSHRNAGDRLLASRA
                     PNRSSSPLKLEAVE"
     gene            698..2206
                     /locus_tag="VFV61_02700"
     CDS             698..2206
                     /locus_tag="VFV61_02700"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HEX4899410.1"
                     /translation="MTQPARSFTLKSQAISDRGLNERRPLNEDSFLADSTRRIFVVAD
                     GVGGAEAGEVASKTAVEVLDEAFRHQEEGADIEDLMELAIQRANASIHQMAQDNARFS
                     MMATTVVALHLKGNVATIGHVGDSRLYRLTPEGDIVRETEDHSVVEEEVRAGRMTAEQ
                     AANHPSKNVISRALGAEDTVEVDLKTFEVEDGTEFLLCTDGITRHISDGELRQLLVIH
                     DDLDALCKELKQRCYERGAEDNLTAVVVRCGGHILAHERAEDLQRTISPETEMFRAAA
                     SAPAVAEVDLEDRTLDALVPPSRTSFPAADQSAPVTRQPLNIPEREHNSGGFAKSVGR
                     LVLFLMFLAVVAAAFYGGRRYKGPIPFLKEPADALVQASPSPTAEDPQAKFERTRADI
                     DSEPQAWLSGEMTRELAAQNILNPLDSVDPGFLYLYGRASMLSGNSADAAKAFEQAII
                     RSAAAPSPTNATVKKDATLALAALALKSDPDKAKALTYLDDLVPKPSPTVSP"
     gene            2335..3885
                     /locus_tag="VFV61_02705"
     CDS             2335..3885
                     /locus_tag="VFV61_02705"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HEX4899411.1"
                     /translation="MPELKLQQSRLDGRYDVLECLGRGSYAEIYVAKDNAAAEDAPST
                     VVIKALNIFLQETPDAELEHTLVANFQNEAVALDLVRHPHIISRLGHGTAIDLGGTTF
                     HYIVLEYMPGGDMAALSRHHPLSIEKAVGYLEQVCSGLAHAHMCGVIHRDIKPQNLLL
                     TSDRKTVKIADFGVARFEATEGAITRVGTNIYAAPEHNPLVQTAPLAADALAKQEPLT
                     VAADIYSLAKTTYSLLAGEAPRRFAHEPITAWPEAISGHYWSEPVLRVLEKATQTRPE
                     ERQQSVAEFWEELADAVLPPTQPLSVAEAIKRRPSSDWMLEAEEITQAPPRPRFEPGL
                     PVATAAVGGEVISTAKRPKIVVPVSMIPGPLAAAPRASAKDIVRAFPDQPRNGKANLP
                     RQPKATSRSKRFLVALILIVCFSGMLLATHRYVTSTWNPLLRIPQLENVFVVGREGVT
                     TTDVNLRGDPSSTNTAIGLAETGSRVKVLAVNNNWYEVQVTQHGRPKTDPYTSDRGWL
                     NKRFVKLD"
BASE COUNT          905 a         1141 c         1195 g          890 t
ORIGIN      
        1 ctacgctcgc ccgaccgatt caggcgaccc tcccatcgcg aattcagcaa cgctttcatt
       61 tgtggcttga tggtttcgat cctccatctc cggaagtttc ctggtggaag aaagactatg
      121 acgaggcgct gaatcgtact ccggagctga aggagattgc gcagcagagt cccgagctac
      181 aaaagactgt caatgtcgac atctggttcg ataagcttgc gttccaaccg gcgcaggctg
      241 tctttggcat cacttccggg aaaacaaccg gacgtggttt gggtctcggc tttccggaag
      301 tgattcccat cgccgactcc gactacgtgt atgcggccat cgccgaggag accggcctgt
      361 tcgggggcgc gatcattatt ctggcggtaa ttatcttcgt cgctgccggc gttcgcactt
      421 cgctcgacgc gcgtgatatg ttcacgaaac tctgtgcggc aggtttaacg gcattcgtgg
      481 gttttcaagc cctggtaaat attggcggga ttctgcgagc gctgcccatg accggtatta
      541 cccttccgtt cgtcagccat ggcgggttta gtttgattac cagctatgcg atgctaggca
      601 tgcttatggc tttctctcac cgcaacgcag gggacaggtt gttggccagc agagcgccca
      661 atcgaagctc ttcaccactc aaactggagg cggtcgaatg acgcagccgg ctagaagctt
      721 taccctgaaa tcacaggcca tctccgatcg cggtctaaac gaaagacgcc cgcttaatga
      781 ggattcgttt ttggccgata gcacgcgccg tatctttgtc gtcgctgacg gcgtcggcgg
      841 tgctgaagcc ggcgaggttg cttccaagac cgcagtcgag gtgctggacg aagcttttcg
      901 gcatcaggag gaaggcgcgg acatcgagga tttgatggag ttggcaatcc agcgcgccaa
      961 cgcttcgatc catcagatgg cgcaggacaa cgcacgcttc tcgatgatgg ccacaactgt
     1021 ggttgcgctg cacctgaagg gcaacgttgc cactatcggc cacgtcgggg attcaaggtt
     1081 gtaccgctta actccggagg gcgacatcgt tcgcgaaacc gaggaccact cggtggttga
     1141 agaagaagtg cgcgcgggtc ggatgactgc cgagcaggct gcaaatcatc cgagtaagaa
     1201 cgtcatcagt cgcgccctcg gcgccgaaga caccgtcgaa gttgatttga agacctttga
     1261 agtcgaggac ggcaccgagt ttctactttg caccgacggc atcacccggc atatttccga
     1321 tggcgagtta agacagttac tcgtaattca tgacgacctt gatgcgctct gcaaagagtt
     1381 gaagcagcgc tgttacgaac ggggcgcgga agataatctg acggcagtgg tagttcgctg
     1441 cggcgggcac attcttgcgc acgaacgagc cgaagacttg cagcgcacta tctctccgga
     1501 aacggaaatg ttccgcgcgg ctgcaagtgc tcccgcggtg gccgaagtcg atcttgaaga
     1561 tcggactctc gatgctctgg tgccgccgtc tcgcacttcc ttccccgctg cagaccaatc
     1621 cgcgccggtc actcggcaac cactgaacat acctgagcgt gagcacaatt ccggtgggtt
     1681 cgccaagagc gtcggccgtt tggtcctgtt cctaatgttc ctggccgttg tcgcggctgc
     1741 gttttatggc ggacggcgtt acaaaggtcc gataccattt ttgaaggaac cagccgacgc
     1801 cctcgtgcaa gcctcgcctt cgcccacagc ggaggacccg caggcaaagt ttgagcggac
     1861 aagggctgac atagacagcg agccacaagc ctggctgagt ggcgaaatga cgagggagtt
     1921 agcagcgcag aacattctga atccgctcga ttcagtcgat ccaggtttcc tctatctcta
     1981 cgggcgcgcc tccatgttgt cgggaaacag cgccgacgcg gcgaaggctt ttgaacaggc
     2041 aattattcgc agtgccgctg caccgtcgcc aacaaatgcc acggtaaaaa aagacgccac
     2101 ccttgcgctg gcagccctgg ctttgaaatc tgatcccgac aaggccaagg cgctgacgta
     2161 tctcgacgat ttggtgccta agccctctcc cacggtttcc ccttaaagct tatgaatgaa
     2221 gcgcattcgc ttctcgccca aacccccgcg tgcttttgcg aaatattttt gactgggcgc
     2281 ggggattttt gcgtatagag catcaaagag agcgctgttt gaagattgct gtaaatgcca
     2341 gaacttaagt tacaacaaag ccggttggac ggtcgttacg acgtactcga gtgcctgggg
     2401 cgcggcagtt atgccgagat ctacgtagca aaggacaatg ccgcggcgga agacgcaccg
     2461 tccacggttg taatcaaagc cctcaacatc ttccttcagg aaaccccgga cgctgaactg
     2521 gaacacaccc tcgttgcaaa cttccagaac gaagccgttg ctcttgacct cgttcgtcac
     2581 ccgcacatca tcagtcgttt gggacacgga acggcgatcg atctgggcgg cactacattt
     2641 cactacatcg tccttgagta catgccgggc ggcgacatgg ccgcgctctc gcgacatcat
     2701 ccgctctcga tcgagaaggc tgtcggctat ttggaacagg tctgttccgg cttagcgcat
     2761 gcgcatatgt gcggggtaat tcatcgcgac atcaagcccc agaacctgct tctgacgagt
     2821 gatcgcaaga cggtaaagat tgctgacttc ggtgtcgcgc gctttgaggc gaccgaaggt
     2881 gcgattacgc gagtcgggac aaatatctat gctgccccgg aacataatcc gctcgtccag
     2941 accgctccgc tggctgcgga cgcgctcgcg aagcaagaac cattaacagt cgctgccgat
     3001 atttattcat tggccaagac gacgtacagc ttgcttgccg gagaggcgcc gcggcgcttt
     3061 gcgcatgagc cgattacagc ctggcccgaa gcgatcagcg gtcactactg gtccgagccg
     3121 gtgctgcgtg tgttggagaa agccacgcag acgcgccccg aagaacgaca acagtcagtc
     3181 gctgagttct gggaagagtt ggccgatgcc gtgttgcctc cgacgcagcc cttgagcgtg
     3241 gctgaggcga tcaaacgacg gcccagttcc gattggatgt tggaagctga ggagatcacc
     3301 caggcgccgc cccggccgcg cttcgaaccg gggttgccgg tggcaactgc ggctgttggc
     3361 ggcgaggtga taagcacggc caagcgtccc aagatagtgg ttcccgtgag tatgattccg
     3421 ggtccgttgg cggctgctcc ccgcgcttct gcaaaggaca tcgttagggc ttttcccgat
     3481 cagccgcgca atggcaaggc taaccttcca cgtcagccca aagcgacgag cagatcaaaa
     3541 cgatttctcg tggcgcttat actgattgta tgtttctccg gaatgctgtt ggcgacgcat
     3601 cgatacgtga cttcgacctg gaacccgctc ttaagaattc cgcagctgga gaatgtattt
     3661 gtggtcggtc gggagggtgt tacgaccaca gacgtgaacc taagaggcga tcccagctcg
     3721 accaatacag cgattggact tgcagagacc ggctcgcgtg tgaaagtgtt ggcggtgaac
     3781 aataactggt atgaggtcca ggtgacgcag catggccggc ccaagaccga tccctacact
     3841 tcagatcggg gctggcttaa taaaagattc gtgaaactgg actaatatca ttgccgattg
     3901 aacattgcca attgccaatt ggtcttatgc aaagctatca aattggcaat cggcaatcgg
     3961 caattggcaa tctattgagg agcgacaatc attgggcttg attgaatcaa ttagaaagtg
     4021 gattgacggc gacaccgaca ccgatttgtt ggaagcggct gatgagcacg cgaaaccgcg
     4081 tcgcatctgg gaagagttcc tggtgaagat cgcccgcgaa gtcgaggcgg c
//
LOCUS       DASUEX010000164         2084 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_488130, whole genome shotgun sequence.
ACCESSION   DASUEX010000164 DASUEX010000000
VERSION     DASUEX010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488130"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2084
                     /locus_tag="VFV61_02710"
     CDS             <1..>2084
                     /locus_tag="VFV61_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899412.1"
                     /translation="LYDEALGQRPWKGIQKEFVSRYSRYLRSIKPQAGKTEAELKESP
                     EYQDLAAQAQAALDQVKPETSEIDHKVVQIQRQLDAVTDPFQNQRGRLTVISYNIETA
                     SGAAKEKYRKEAERKRAEVVDVELPAGDGSGAVAVQKMNYQQLEKFYNDLRDEKAQLL
                     GKKAELLKEPTEVAKKRDDYLKHHLIGLGPSQIDGLLRKMDAFDYSILGHQISVNSSG
                     IVDRCQVCHVGVREPLDLKPEDLAPDGPGKKPDSLARAFVSHPDKELLRVHNPDRFGC
                     SGCHWGNGRATTSDIKGHGRHKYWLWPLFEKENIEAGCQQCHARDRVTQGAETLNLGR
                     DLFAERGCVGCHRYEGFDRETDALSAARQNTTQLEEQILANEKQIREDTEAANQAADD
                     TEAQRLLAHAESLRVTNSLLAARKDQVNLQSKYLMQDQKKVGPNLKDVKLKLRKEWIP
                     VWLKDPQAFRPGTKMPQFWRFGIDQDGEEQIKAISAYLWQESFDAKPAEQPRGDAAHG
                     KELFETRGCLACHSIGEPDSAVGGHFAANLQKIGEKANYEYIVRWIHNPRERWAPYCP
                     KEKRDLTAADYSQHNLPYVFDTELHSRCPNDGTELQVQNMTVMPNFRLADSETRDIAT
                     YLFSLSSAPSYPDASYMDDPNLKPKGQALIKQYGCAGCHEIKGFEEEQRIGKELTVEG
                     ATPIERLDFALLT"
BASE COUNT          537 a          548 c          592 g          407 t
ORIGIN      
        1 ctttacgatg aggcgcttgg tcagcgtccc tggaagggta ttcaaaaaga atttgttagc
       61 cgctacagcc gctacctgag aagcatcaag ccgcaagccg gcaagaccga agcggaactt
      121 aaagagagcc cggagtatca agacctggca gcgcaggcac aagctgcgct tgaccaggtg
      181 aaaccggaga cttccgaaat cgatcacaag gtggtgcaaa ttcaacggca gctcgacgcc
      241 gttacagatc cttttcagaa tcagcgcggc cgcctgaccg taatcagcta caacatcgaa
      301 acggcttccg gagccgcgaa agagaagtat cgtaaagagg cggagcgcaa acgggctgag
      361 gttgtggacg tcgagctacc cgccggcgat ggttcaggag cagttgcagt tcagaaaatg
      421 aactatcagc aactggaaaa gttctacaac gatctgcgcg atgaaaaggc ccaactgctg
      481 ggaaagaagg cggagctgct caaagaaccc accgaggtgg ccaagaagcg cgatgattat
      541 ctgaagcatc acttgatcgg actgggcccc tcacaaatcg atggcctgct gcgcaagatg
      601 gatgcttttg attattccat cctcgggcat cagattagcg tcaactcctc gggtatcgtg
      661 gatcgttgcc aggtctgcca cgttggggtt cgtgaacctc tcgatctcaa acccgaagac
      721 ctggcgcccg acggtcccgg aaagaagccg gacagcctgg ctcgagcgtt cgtgagtcac
      781 cctgataaag agttgttgcg agtgcacaat cccgatcgct ttggttgttc cggatgccat
      841 tggggcaatg gccgcgcgac caccagcgat atcaagggac acggccggca caaatattgg
      901 ctgtggccgc tcttcgaaaa ggaaaacatt gaagccggtt gccagcagtg ccatgcgcgc
      961 gaccgtgtca cgcagggggc cgaaactcta aatctgggtc gcgatctctt cgccgagcgt
     1021 ggttgcgtcg gttgtcaccg ttacgaaggc ttcgatcgtg aaacagatgc cctctcggca
     1081 gcacggcaga atactacgca gctcgaagag cagatcctgg cgaacgagaa acagatccgc
     1141 gaagacactg aggctgcgaa tcaggctgct gacgataccg aggcacaaag actattagcc
     1201 cacgccgagt cgttgcgagt cacgaatagt ctgctggcgg cgcgcaagga tcaggtgaat
     1261 ctgcaatcga agtatctgat gcaggatcaa aagaaagtcg ggccgaatct gaaagatgtg
     1321 aagttgaaac ttcgcaaaga atggattcca gtatggctca aagatccgca ggctttccgt
     1381 ccgggaacca agatgccgca gttttggcgc tttggcattg atcaggatgg cgaggagcag
     1441 atcaaagcta tttcggctta tctctggcaa gaaagctttg acgcaaaacc cgccgaacag
     1501 ccacgcggcg atgccgcgca cggcaaggag ctcttcgaaa ctcgtggttg tcttgcctgt
     1561 cattcaatcg gcgaaccgga tagcgccgtc ggcgggcact tcgccgccaa cctgcaaaag
     1621 attggcgaga aggcgaacta cgaatacatc gtccgctgga ttcataatcc gcgggaacgc
     1681 tgggctcctt attgtcccaa ggaaaaacga gatctaactg cggcggatta ctcgcaacat
     1741 aatctgcctt acgtctttga tactgaactt cattcgcgtt gtccgaacga cggaaccgag
     1801 ctgcaagtgc agaatatgac tgtcatgcca aacttcaggc tggcagacag cgaaacgcgc
     1861 gacatcgcga cctacctttt ctcgctctcg tcggcgccct cgtatcccga tgcgtcttac
     1921 atggacgacc cgaatctgaa acccaaaggt caggcgctaa taaaacagta cggctgcgct
     1981 ggttgtcatg agatcaaagg gttcgaagag gaacagcgaa tcggtaagga gttgacagtc
     2041 gaaggtgcaa caccgattga gcggttggac tttgctttgc tcac
//
LOCUS       DASUEX010000165         3975 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1161642, whole genome shotgun sequence.
ACCESSION   DASUEX010000165 DASUEX010000000
VERSION     DASUEX010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3975
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1161642"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1355)
                     /locus_tag="VFV61_02715"
     CDS             complement(<1..1355)
                     /locus_tag="VFV61_02715"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II and III secretion system protein family
                     protein"
                     /protein_id="HEX4899413.1"
                     /translation="MQSPSMKRRVSLVLASLIIMLVNSMVSAQETSYSASFTQNKEPI
                     PVNVLVGQSRVINFDRPVGRFSVSNPEIAEAVLVTPNQVLVNGKGFGQVNFIAWEQSG
                     GEYLVFDVYVRANLSLIDSQMRVLFPKDDIRLSQANGSVVMSGSVGDVKTGAQAQSVV
                     EAAGFKTVNMLSSPVKAATQVQLQVRVAEVSRNRLKDVGSSYTYQGSPGSGTYLNSGG
                     GPSSLSNPPASPLSGIISGALSSSALNMLVMGGNSLAMLRMLQTEGSLRELAEPNLIA
                     MDGQEASFLAGGEFPVPIVQGGSDKTTVSIIFKEYGVRLNFKPTIIDEDHIRLELEPE
                     VSTIDFANGVKFDGFLIPALKTRRAKTGVELRDGQSFALAGLLDNSEIKSLSKVPVLG
                     DIPVLGSLFRSKSFQKNETELMFIVTAMLVKPVNRDDLPQMRGIDGLKKASPLGIEPK
                     G"
     gene            complement(1447..2346)
                     /gene="cpaB"
                     /locus_tag="VFV61_02720"
     CDS             complement(1447..2346)
                     /gene="cpaB"
                     /locus_tag="VFV61_02720"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HEX4899414.1"
                     /translation="MRNKRFFIVLAGALLFGLLAAVSVSRYLSSAQAYTKSLRGVAVA
                     KVAIPIGTKIIAEQITLVQFPQESTPDGAFDSIEKLAGRVAVVNIAPKEPITEARLAP
                     EGTAGGLSAIIPEGYRAMTVKVDDVVGISGFIMPGTLVDVVVVIVPTGNGAIAQDPIS
                     KIVLQNIKVLANGQNIDKPESEREANSVKAVTLLVTPEQAEKLALASSEGKLQLVMRN
                     SIDQGDEQTIGINKRTLLGGDVATPQPAPGSLKSERPADQKPAPRRVRVQSEAKPAPA
                     APPAPKASVEMIEGAKKRSVDFP"
     gene            complement(2394..2915)
                     /locus_tag="VFV61_02725"
     CDS             complement(2394..2915)
                     /locus_tag="VFV61_02725"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HEX4899415.1"
                     /translation="MSATVVTIALLLPLAVIITYHDVRYRRIPNAYVLAALLGGVAIN
                     GIFGGLSGLAASLGGCALAFGLMFMLHVFGAMGAGDVKLFAAIGAITGAHLVLPTFLV
                     VVLTGGVLAMVTIIRAGVLITTMHRVLQILVGMLPGWEMPKFAIPTDRSHTIPYGVAI
                     TIGSIISTAVFRA"
     gene            complement(2957..3115)
                     /locus_tag="VFV61_02730"
     CDS             complement(2957..3115)
                     /locus_tag="VFV61_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899416.1"
                     /translation="MIMNFLRDETGLELSEYAVAAALVAMACALAFTTLGVAIGDKIN
                     TLAATISG"
     gene            complement(3227..3385)
                     /locus_tag="VFV61_02735"
     CDS             complement(3227..3385)
                     /locus_tag="VFV61_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899417.1"
                     /translation="MIKRFFRDETGLELSEYAVAAALIAIAVVGAFTALGTAISQKVN
                     TLAATITG"
     gene            complement(3475..3723)
                     /locus_tag="VFV61_02740"
     CDS             complement(3475..3723)
                     /locus_tag="VFV61_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899418.1"
                     /translation="MKELKAKELISLAGIAVTAPILALAILVSARRAAVADYDMLLVI
                     LSLCGAVVSGLNGFGRRTIKTPYDQSRKEPRPRVAIPS"
     gene            complement(3792..>3975)
                     /locus_tag="VFV61_02745"
     CDS             complement(3792..>3975)
                     /locus_tag="VFV61_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899419.1"
                     /translation="AGRQKQVLRKRWPGNLPSERGGPARDSGLSTAGYKTISFGFHES
                     AIVQGFTVIGLLARSL"
BASE COUNT          863 a         1094 c         1116 g          902 t
ORIGIN      
        1 tcgcccttcg gctcaatacc caggggcgac gcttttttca gtccgtcgat gccgcgcatc
       61 tgtggcaggt catcgcgatt caccggctta accagcatcg cagtgacgat gaacatcaat
      121 tcggtttcgt tcttctgaaa cgatttcgag cggaagagcg aacccaatac cggaatgtcg
      181 cccagcacgg gaaccttgga aagcgatttg atttcgctgt tgtctaagag accggccagt
      241 gcgaacgact gaccatcgcg cagctcgacg cccgtcttgg cgcggcgggt ctttaacgcg
      301 gggatcagga agccatcaaa cttgacgccg ttagcaaaat cgatggtcga gacttcgggt
      361 tccaattcga gacggatgtg gtcttcatcg ataatcgtcg gtttgaaatt caaccgcacg
      421 ccgtattctt tgaagatgat cgatacggtg gtcttgtcgc tgccaccttg cacgatcggc
      481 acgggaaact caccgccagc caggaagctg gcttcctggc catccatggc tatcaagttc
      541 ggctcggcaa gttcgcgaag tgatccttcg gtctgcagca tgcgcaacat ggccaaagaa
      601 tttcctccca tcacgagcat gtttaaggca ctgctcgaga gcgcaccgga aatgatgccg
      661 ctcaacgggc tggccggagg attgctaagc gagctcggtc caccaccact gttcaggtaa
      721 gtgccgctgc cgggactacc ctggtatgtg taagagctgc ccacatcttt cagacgattg
      781 cgactgacct cggctacgcg aacttgcagt tgcacctggg tcgcggcctt caccggcgag
      841 gaaagcatgt ttacggtttt gaagccggct gcctcgacga cgctttgagc ctgcgctcca
      901 gtcttgacat caccgacgct gccagacatc accaccgagc cgttggcctg actgagacgg
      961 atgtcatctt tcgggaagag gacgcgcatc tgcgagtcga ttagcgaaag gttggcgcgc
     1021 acataaacgt cgaacaccag atactcgccg cctgattgtt cccaggcgat gaagttcact
     1081 tggccgaaac cttttccatt gacgagcact tgattaggcg tgacgagcac ggcctcggcg
     1141 atctcgggat tcgagacgga aaagcggcct acgggtcggt cgaagttgat aacccgcgac
     1201 tgcccgacga gcacgtttac aggaatgggc tctttattct gcgtgaagga agcgctgtag
     1261 gaggtttcct gcgcgctaac catggaatta accagcataa taatcaacga cgctaacacg
     1321 aggctgacgc ggcgtttcat tgagggactt tgcatggtct gttgctcctt aaaattagtg
     1381 gcagtcgatg ttgtttgggc tgacgccgtc atcaacttgc aatccttttt tgagttccga
     1441 ctctccttac gggaagtcaa cacttcgttt cttggcgcct tcaatcatct cgactgaggc
     1501 ttttggtgcg ggtggcgcag ccggtgccgg ctttgcttct gattgcaccc gaacacgacg
     1561 cggtgcaggt ttttggtcgg cgggccgttc actcttcaat gaaccaggcg caggctgcgg
     1621 agtagcgaca tcgccgccca acagagttct cttgttgata ccgatagttt gctcgtcgcc
     1681 ctggtcgata gagttacgca tcacgagctg gagcttgcct tcactggaag cgagagcgag
     1741 cttctcagcc tgttcaggcg taacgagaag cgtgacggcc ttgacgctgt tggcttcacg
     1801 ttcgctttcc ggcttgtcaa tgttctgacc attggccagc accttgatgt tttgcagaac
     1861 gatcttggag atcgggtcct gagcgatcgc gccgtttccg gtgggaacga tgacaaccac
     1921 aacgtcgacg agtgttcccg gcatgatgaa gcccgagata ccaacgacgt cgtcgacctt
     1981 aaccgtcatt gcgcgatagc cctcaggaat gatcgcggag agacctcccg cagtaccctc
     2041 aggcgctaga cgcgcttccg tgatgggctc ttttggcgca atgttcacga ctgccacgcg
     2101 tccagcgagt ttctcgatcg aatcgaacgc gccgtcggga gtcgattcct gggggaattg
     2161 caccagggta atctgttcag cgatgatttt ggttccaatc ggaatcgcta ctttggcaac
     2221 cgccacgccg cgtagactct tggtgtaagc ctgcgcgctc gacaaatagc gactgacaga
     2281 aaccgccgca agcagcccaa acaaaagggc gccggctaac acgatgaaaa agcgtttgtt
     2341 gcgcatgatc tgaatctccg tggtcagttg acccgacaaa gggggtttgc gctctaggcg
     2401 cgaaaaaccg ctgttgaaat gatgctgcca atcgtgatgg ctacgccgta gggaatcgta
     2461 tgagagcggt cagttggaat ggcgaacttg ggcatttccc agccgggcaa catccccacg
     2521 agtatttgca agactctgtg catcgtggtg attagcacgc cggctcgaat aatcgtgacc
     2581 attgcgagca cacctcccgt cagtacgacg acgagaaatg ttggaagcac aagatgcgcg
     2641 cccgtaatcg caccgattgc tgcgaagagt ttcacgtcgc cggctcccat tgcgccaaag
     2701 acatgcagca tgaacatcag gccgaaggcc agggcgcatc cacctaaact ggcggccagt
     2761 cccgacagtc ctccgaagat gccgttgatc gccactccgc caagcagtgc ggcgagcacg
     2821 taagcgtttg gaatacgacg gtaccgcacg tcgtgataag tgataatcac tgcgagcggc
     2881 aggaggaggg cgatcgtgac aaccgttgcc gacatttgtt agggcacctc gaggaccgtt
     2941 gggggaagaa tttgtattag ccgctgatcg tcgcggccag cgtgttgatt ttgtcgccga
     3001 tagcaacgcc gagagtcgtg aaagcgagag cgcacgccat cgccacgagg gcggcggcta
     3061 ccgcatattc ggaaagctca agtccagtct catctcgaag aaagttcata atcatggttt
     3121 tcactccctt gctggaggat cttttggcgg aagagccgat aacgcgcttt acccgagttt
     3181 gaatctcagc taaagcgcgc tcgggcccat tttcgctaag ttggacttat ccggtgatgg
     3241 tcgcggcaag cgtgttaacc ttctggctaa tggctgtgcc taaagcggtg aaagcaccga
     3301 caacggcgat cgcaatcaag gctgcggcaa ctgcgtactc cgacaactca agaccggttt
     3361 catcgcggaa gaatctctta atcattttgt tgctccttct aagttggttt agggtgattg
     3421 atccttcacc attcatgtcc gtgaattgcg aaggcttgag agttagcgca aagctcagga
     3481 tggaatggcc actcttggcc tcggttcctt gcgactctgg tcatagggcg ttttgatcgt
     3541 gcggcgacca aaaccgttaa gcccacttac cacagcgcca cacagagata agatcactag
     3601 caacatatcg tagtcagcca cggccgcacg gcgagcactg accaggattg ccagtgccag
     3661 gattggcgcg gtaactgcga ttcctgcgag cgatatcaac tctttagctt ttagttcctt
     3721 catgtgtgcc ttggtgtgta tttctctctt gggtgatctc gcgacgcatt gcctcgcgac
     3781 ggggatgacc attacaagga ccgtgccagc aaaccgatca cagtaaagcc ctgcacaatt
     3841 gcactttcgt gaaagccgaa gctgatcgtc ttgtagcctg cggttgacaa gcccgagtcc
     3901 cgagcggggc cgccacgttc gcttggcagg ttcccgggcc accttttcct gagaacttgc
     3961 ttttgtcgcc cggcg
//
LOCUS       DASUEX010000166         2035 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_362605, whole genome shotgun sequence.
ACCESSION   DASUEX010000166 DASUEX010000000
VERSION     DASUEX010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362605"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(202..1599)
                     /gene="boxB"
                     /locus_tag="VFV61_02750"
     CDS             complement(202..1599)
                     /gene="boxB"
                     /locus_tag="VFV61_02750"
                     /EC_number="1.14.13.208"
                     /inference="COORDINATES: protein motif:HMM:TIGR03225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0010128 - benzoate catabolic process via
                     CoA ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA 2,3-epoxidase subunit BoxB"
                     /protein_id="HEX4899420.1"
                     /translation="MNLYERIPNNVNLAEDKKLQRALEKWLPDYLDWWKEMGPEGFQE
                     KDVYLRTAISVEPDGWAHFDYVKMPDYRWGIFLKPAAPDAKIGFGDNYGQPAYQEVPG
                     EFRNSLRRIIVTQGDTEPASVEQQRQLGKTCPSLYDLRNLFQVNVEEGRHLWAMVYLL
                     QRYFGRDGRDEADALLERRSGNPDKPRILEAFNQPCDDWLNFFCFTMFTDRDGKYQLS
                     ALAESGFEPLARTCQFMLTEEAHHMFVGDTGIGRVVKRTAQLMKEGDVRELGGIPLCI
                     IQRYINFWYSYSLDLFGGEISSNSADFFAAGLKGRYKEQEQYQDHSAADETHTMTIVE
                     NGALNSREVPLRNALNEVLRNEYFKDCEKGLARWNKVLADEGLSNRLYLPSTRFHRHV
                     GEYAGHHFDLDGNLISAEEFASRQADWLPTLEDREYVRSLMHPVTEPGKIANWIAPPA
                     TGIKGKPFEFEYVRL"
     gene            complement(1665..>2035)
                     /locus_tag="VFV61_02755"
     CDS             complement(1665..>2035)
                     /locus_tag="VFV61_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899421.1"
                     /translation="TRLQSRFLAEPERVNEITNRVGAFDTEAAEAAGLITFAPDDLDW
                     EDEIRVAIEERTSLSPDALTGMEASLRFAGPETMDTKIYGRLTAWQNWIFQRPNAVGE
                     HGALTNYGKPTQAQFDFKRT"
BASE COUNT          435 a          581 c          522 g          497 t
ORIGIN      
        1 atttcgaatt tcgaatttcg aatttcggat gtcgcagggt cgtggctaac atcaaggtct
       61 tcggcaccgc caacatcagc gcggtggatc gtcgggttgt ccaaatctct attcacgatt
      121 ggtaccggtt cggatgtcga atttcgaatg tcgaatgtcg aatttcgcaa ttcacaattc
      181 gcaattcgca attcgcaatt cctaaagcct cacatactca aactcaaagg gtttgccttt
      241 gatcccggtc gctggaggtg cgatccagtt cgcaatcttt ccgggttccg tcaccgggtg
      301 catgagactc cgcacatact ctcgatcttc caatgttggc agccaatcgg cttggcggct
      361 tgcaaactcc tcggcgctga tcagattgcc gtcgaggtcg aagtgatggc ctgcatattc
      421 gccaacgtgg cgatgaaacc gagtgctcgg tagatacagc cggttgctca gtccttcgtc
      481 cgccagcact ttgttccaac gagcgagacc cttctcgcag tccttgaagt actcattccg
      541 cagtacctca ttcaatgcat ttctgagggg cacttcgcgg ctgttgagtg caccgttttc
      601 gacaatcgtc atcgtgtgtg tttcgtctgc ggcgctgtgg tcctgatact gctcttgttc
      661 cttgtaacgg cccttcagac cggcggcaaa gaagtcggcc gagtttgagc taatctctcc
      721 cccaaacaga tcgagcgagt agctatacca gaagttgatg taccgctgaa tgatgcacaa
      781 cggaatgcca ccaagctcac gcacgtcacc ctctttcatc agttgcgccg tgcgtttgac
      841 gacccggccg atgccggtat cgccgacaaa catgtggtgc gcctcctctg tcagcatgaa
      901 ctggcaggtc cgggcaagtg gttcaaaacc agactctgcg agagcagaaa gttgatactt
      961 cccatcgcgg tcggtgaaca tcgtgaagca gaaaaagtta agccagtcat cgcagggctg
     1021 gttaaaggct tccaggatgc gcggcttgtc aggattgccg ctgcgacgtt caagcaaggc
     1081 atcggcttca tcgcgaccat cccggccaaa ataacgctgc agcagataga ccatggccca
     1141 caggtgacgg ccctcctcga cgtttacctg gaaaagattt ctcaggtcat aaagtgaggg
     1201 acaggtcttg ccgagttggc gttgctgctc aactgaagca ggttcggtat caccctgtgt
     1261 gacgataatc cgccgtaacg aattccgaaa ctcaccgggt acttcctgat aggcgggctg
     1321 gccatagttg tcaccaaaac cgatttttgc gtcaggcgcc gccggcttga ggaaaatgcc
     1381 ccagcgataa tccggcatct tcacatagtc gaaatgcgcc caaccatcgg gttcgacgct
     1441 gatcgccgtg cgcaggtaga catccttctc ctgaaaccct tcgggtccca tctctttcca
     1501 ccaatccaaa taatccggca gccacttctc cagcgcgcgt tgcagcttct tgtcttcggc
     1561 aagatttacg ttgttaggaa tgcgctcgta caggttcata aatgcctcgc tcagaaaact
     1621 ccaatgttat tgggccatcg acgccgtgcg tccccagtcc ttctttaagt tcgtttgaaa
     1681 tcaaactgcg cttgcgtcgg cttgccgtag ttggtcaatg ccccgtgttc gccgacagcg
     1741 ttcggtcgct ggaaaatcca gttttgccag gcagtcagcc gtccatagat cttggtgtcc
     1801 attgtctccg gaccggcaaa tcgcaaggat gcttccattc cggtcagggc gtccggtgac
     1861 agcgaggtcc gttcctcaat agcgacgcgg atttcgtctt cccagtccag gtcatcggga
     1921 gcgaaagtga tcaggccggc agcttccgcg gcttcggtgt caaaagcacc aacacgattc
     1981 gtgatctcat tcactctctc tggctccgcc agaaagcgcg actgcagtct tgtca
//
LOCUS       DASUEX010000167         4176 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_362652, whole genome shotgun sequence.
ACCESSION   DASUEX010000167 DASUEX010000000
VERSION     DASUEX010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4176
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362652"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            254..1228
                     /locus_tag="VFV61_02760"
     CDS             254..1228
                     /locus_tag="VFV61_02760"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEX4899422.1"
                     /translation="MSQSSEMSGKAEFDPSVTTCAKCGTPMPLGLRFCRNCGFRLGEG
                     PAEYTETVRFPNGQLGATQQPFSPNYAAPLAHAPAGAFGKPRKRMSGMSWMFIGLLVF
                     FLAAAAFTAIITPIRRNVTTISVPDKPRAFIGVNDFETGEGGVTFNNVEPPGSPADKA
                     GLVGGDIITSFDGHVVTTDDEMTELMGDTTIGKPVDVIYLRDGEQKTTKLTPITREDY
                     QRLEREFSNRPGGKGLFGFDDDNTERVAIAGTKLFGVKLNEINQNRPADMAGIKEGDI
                     VTEFDGVPIRTPEELSARVRRAVPYNTIKVVVMRGTEMLEIPVKMGKQ"
     gene            complement(1258..1629)
                     /locus_tag="VFV61_02765"
     CDS             complement(1258..1629)
                     /locus_tag="VFV61_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899423.1"
                     /translation="MVNRLDQIKEILATYKKHGWRLRQVLLSPQTRAEIVDDAWAGDA
                     QIEAREFDALWFSRASHGNREAWELRLVATTQYALFETFEADEPEAAQEEVRREMEAR
                     MKEKVSGAWSQVSDRSNDPNS"
     gene            1887..2711
                     /locus_tag="VFV61_02770"
     CDS             1887..2711
                     /locus_tag="VFV61_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899424.1"
                     /translation="MSPNPETTAAADKKIVVPTPFYEALRKALKKRKTTIERICPIGD
                     PVARRILEEYGAVFLAHKKVLPPPACVFTNEDQVTAFQNAAGSAAETIADAEIELQPE
                     AMKALLKAREEAQKEALDITPRGGEEAARRNFEDSMRLWDTRFLPALDYWMSQGRLSS
                     DQVNRLKALPLHDQIGEVLELEKVGIFFSKDLSKSILYSIAAPGTSQHIAMLALDVTE
                     FDNPRVREILAQHGWFQTVLSDLPHFTYLGLKEKDLPKQGLKSVIVDGQTFWVPNV"
     gene            2981..>4176
                     /locus_tag="VFV61_02775"
     CDS             2981..>4176
                     /locus_tag="VFV61_02775"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX4899425.1"
                     /translation="MDVPQFIAGRFRIECEIGTGGMGTVYRATHLGLERPVAVKIIKQ
                     EFAGDRDVADRFLREARTMARLRHTHAAMIFDAGNLPDGRHFIIMEFVEGKTLSQALA
                     RETRFSPEQAVKIAVQICEVLEEAHQLGIIHRDLKPSNIMLNERGVRVLDFGVAKVLV
                     TADTTATHATTGSGQIVGTPRYMSPEQCLGQRVGARSDLYSLGVVLYEMLAGRPPFVD
                     VLPSAVLVKQAIAPPPPLPQLRPDIPRQLALVVHSLLAKRPEDRPRTAAAARLLLEKS
                     LVPRARELPQTDPFSSTLAVVAERGNIVSRLVAPLATVIFLGALLFVWGGNGETASQA
                     AANLLAASKLGSESTAETNHRDERTIPSPVLANSLTLDAGRSRPATLDLARAESIAAR
                     VSRNTVR"
BASE COUNT          966 a         1167 c         1126 g          917 t
ORIGIN      
        1 cgccacctcg ggataagcaa gattccgcac acggatcacg cgaaatgcaa ccgaaacgcg
       61 aggaaacgca ctttttttct ggaacgcacc cccacgattc gggaacttcg cgcggcacta
      121 caaatcacgc tcgtttttga cttactataa gcggtgcggt gtggcgactc gctgatgcaa
      181 ctggcaggca gtttttggcg tatacccatc cggagccgat gtcagcgcca acccttagac
      241 tggtgagagt ctcatgtcgc agagttcaga aatgagtgga aaagcagaat tcgatccaag
      301 tgttactact tgtgcaaaat gcggcacgcc tatgccctta ggtctgcgct tctgccgcaa
      361 ttgtggcttt cgactgggcg aagggccggc tgagtacaca gaaacagtgc gcttcccaaa
      421 cggtcaactc ggcgctacgc aacaaccatt ctcgcccaac tatgcagcgc ccttggcgca
      481 tgcgcccgcc ggcgctttcg gcaaaccacg taagcgaatg agcggtatga gctggatgtt
      541 tatcggtctg cttgttttct ttctcgctgc cgcggcgttt actgccatca tcacgccgat
      601 acgtcgcaac gttacaacga tttctgtgcc cgacaagccg cgcgctttta ttggtgttaa
      661 tgattttgag actggtgagg gaggcgtcac gttcaacaat gtagagccgc ccggatctcc
      721 cgcggacaaa gccggtctcg tcggtggcga catcatcacc agcttcgacg gtcatgttgt
      781 taccactgac gacgaaatga ccgaactaat gggagacact acgatcggga agccggtaga
      841 tgtgatttac ctgcgcgacg gcgagcaaaa gacgacgaag ctaacgccaa tcacgcgtga
      901 ggactaccag cgactggaaa gagagttttc gaatcgtccc ggcggcaaag gtctatttgg
      961 gtttgacgac gacaacacgg aaagagtggc gattgcggga actaagctct tcggcgtaaa
     1021 gctaaatgag attaatcaga accgcccggc cgacatggcg ggcatcaagg aaggcgacat
     1081 cgtgaccgaa ttcgatggcg tgccgatcag gactcctgag gagttgtctg cgcgagtgag
     1141 gagagccgta ccctataaca ccatcaaagt tgtcgtcatg cgcggcactg aaatgctcga
     1201 gataccggtg aagatgggaa aacagtaaaa agggtgtcag gtgacgggta tcgggtttta
     1261 gctgttagga tcattgctcc tatccgaaac ctgagaccag gcacccgaca ccttttcttt
     1321 catccgcgct tccatttctc ttctaacttc ctcttgggct gcctccggct cgtcagcctc
     1381 gaaagtttca aagagcgcat actgagtggt agcgaccaag cgcaactccc aggcttcccg
     1441 attgccgtgc gaggctcgtg aaaaccaaag cgcatcaaac tctcgcgctt caatctgcgc
     1501 atctccagcc caggcatcgt cgacaatctc cgcgcgagtc tgtggactca gcagtacttg
     1561 tcttaggcgc cagccgtgct ttttatacgt ggccaggatt tctttgattt ggtcgagtcg
     1621 gttgaccatc ctatttgtcc acccctatca tcttgctaac aaagatgcag tggcaccagg
     1681 gcgagcaagt ctttctaatt gacttcggtc attctagaga tatcaagaca ggttatcatt
     1741 tcgcagaatc gcaattgtcg gttctagctt ctccgacatc gttaaaacca agttctctcg
     1801 ttctgttctt attagtagca gcgctcctaa gctgcccgcg aagtgcgacc gccaactcta
     1861 taagaaacat ctacccacgc acaccgatga gccccaatcc cgaaactacg gcagccgccg
     1921 acaagaagat tgtcgtgccc accccctttt atgaagcgct gcgcaaagct ttaaagaagc
     1981 gcaagaccac gatagaacgg atttgcccga ttggagatcc agtagcgcga cgcattctgg
     2041 aagaatatgg cgcagttttc ctggcacaca agaaggtgct gccgcctcct gcatgtgttt
     2101 ttaccaacga agatcaggta accgcctttc agaacgccgc cggttctgcg gctgaaacaa
     2161 ttgctgatgc ggaaattgag ttgcagccgg aagcgatgaa ggctttgctc aaagcgcgtg
     2221 aggaagcaca gaaggaagca ctcgacatca ccccgcgcgg cggagaggaa gcagcgcgcc
     2281 gtaatttcga agacagtatg cgactctggg atacaagatt cttgccggcg ctcgattact
     2341 ggatgtcgca gggtcgcttg agtagtgacc aggtgaatcg cctaaaagct ttgccgctac
     2401 atgatcagat aggagaagtc ctggaactag agaaggtggg gatcttcttc agtaaagacc
     2461 tttccaaatc gatcttgtac tcaatcgccg cgcccggtac ctcgcaacat atcgcgatgt
     2521 tggcactcga cgtcaccgaa ttcgataacc cgcgggtgcg agaaatcctg gcgcagcatg
     2581 gttggtttca gaccgtgctt agcgatctcc cgcacttcac ttacctgggg ctgaaggaaa
     2641 aagacctacc gaagcaggga ctcaaaagcg taattgttga tggccagact ttttgggttc
     2701 cgaatgtcta ataggttcaa ggttcaaggc tagactttcg ggcttctcgc tcaacacgtg
     2761 aaccgtcaag acggttgaac ctttaacctg aaaccttaaa cttcaaacct ggagatttca
     2821 ccgacaacga tgctagacta gcgccgctcc aaatccccgg acacaccggg agcgctcccc
     2881 tggatcaaca gcaacttaat aggcctgctt aacgccgcac ggatctgtgc agctcccgga
     2941 gcagtcgccg ctcggccttt atcccgaaag aaggtctgct atggacgtcc cgcaatttat
     3001 cgccggcagg tttcgcatcg aatgtgagat tggaaccggc gggatgggta ccgtgtaccg
     3061 cgcgactcac ctcggtctcg aacgccccgt cgctgtcaag attatcaaac aggagtttgc
     3121 cggagatcgc gacgtcgccg atcgcttcct gcgcgaggcg cgcactatgg cgcgattgcg
     3181 tcatacccac gcagcgatga tcttcgacgc cggcaatctg ccggatggac gacacttcat
     3241 catcatggag tttgtcgaag gtaagacact ttctcaggcc ctggctcgcg agactcgttt
     3301 ttccccagaa caagcggtga agatcgcggt ccagatttgc gaagtgttgg aggaagcgca
     3361 ccagctgggc atcattcacc gcgacctgaa gccgtcgaac atcatgctca acgaacgcgg
     3421 tgtgcgcgtt ctggattttg gagtcgcaaa agttctggtg acggcggata cgactgctac
     3481 gcacgccacg accggctcgg gccaaatagt cggaacaccg cgatacatgt cgccggaaca
     3541 atgtttgggg cagcgcgtcg gcgctcgcag cgatctttac agtttgggag tagtgctcta
     3601 cgagatgctg gctggccgtc cgccgtttgt tgatgtcttg ccgtcagcgg tgttggtcaa
     3661 gcaggcgatc gcgccgccac caccattgcc gcagttgcgt cctgacattc cccggcagtt
     3721 agccctcgta gtgcactcgt tgttagcaaa gcgtccggaa gatcgtccgc gcacggcagc
     3781 agcagctcgt ctcctcctcg aaaagagtct cgtgcctcga gcgcgcgagc tgccgcagac
     3841 agatcctttt tcttctaccc tcgcagtcgt tgccgaacgc ggcaatattg tctctcgctt
     3901 ggttgctccg ctcgctaccg taatcttttt aggagcactg cttttcgttt ggggtggcaa
     3961 cggtgagaca gcctcacagg cggcagcaaa cctgctcgct gcgagcaagc tgggttcgga
     4021 atcaactgca gaaacaaacc atcgcgacga gcgcaccatt ccgtcaccag ttctggccaa
     4081 ctctttaaca ctcgacgcgg gaagatcaag accggcgacg cttgacctgg ctcgagcgga
     4141 gagcatagcc gcgagagttt cgcgcaacac ggtccg
//
LOCUS       DASUEX010000168         2068 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1277902, whole genome shotgun sequence.
ACCESSION   DASUEX010000168 DASUEX010000000
VERSION     DASUEX010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1277902"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="VFV61_02780"
     CDS             complement(<1..398)
                     /locus_tag="VFV61_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899426.1"
                     /translation="MNTRKMTLCLVLCLAISSTAFAQGTRMLRHPSVSRDLVAFAYAG
                     DLWVVARSGGEARRLTSTQGAEVDPYFSPDGSKIAFSATVAGNTDVYVVPTTGGDAKR
                     LTYHPGADRARGWTPDGRRVLFASGRIGAP"
     gene            complement(487..>2068)
                     /locus_tag="VFV61_02785"
     CDS             complement(487..>2068)
                     /locus_tag="VFV61_02785"
                     /inference="COORDINATES: protein motif:HMM:NF013586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEX4899427.1"
                     /translation="SPAGSMYTTVTDMGRFMSALLAGGRGAQGQMLKASTIDQMWTPQ
                     FAPPGQKTGYGIGFGVRELEGRRTVGHGGAIYGFATTLKAMPDDKLGVVVVTTKDSAN
                     AVTNRMADYALTTMLAVRQGKPIPQPETSGPVDPQLARRIAGRYVNGSNSVELIESAG
                     KLSKLSSDGGEQVRLRSLGDALIVDDKLAYGEKILVRDNALVIGDETFKRVEVPKPPP
                     ATVRWRGLIGEYGWDHDILYIFEKDGKLWALIEWFEFDPLEQVSENVFKFPDRGLYDG
                     ERLIFTRDQTGRATQVEAASVVFKRRNVGPEEGAAQLRIKPLRPISELLKEALVAQPP
                     KEAGQFREAELVELNRLDPTIKLEIRYATPNNFLASVFYSEARAFLQRPAAEALVRAQ
                     QKLKQKGYGLLIHDAYRPWYVTKVFWDATPEDKKIFVADPSRGSRHNRGAAVDLTLYD
                     LASGKPVEMVGTYDETTDRSYPDYPGGTSLQRWHRDLLRAAMEAEGFTVYEAEWWHFD
                     YKDWQKYPIGNVVFDKIK"
BASE COUNT          435 a          610 c          568 g          455 t
ORIGIN      
        1 tgaggcgcgc cgatccgccc cgaagcgaaa agcacccgac gaccatcggg tgtccagcct
       61 cgcgctcggt cagctccggg atgataggtc aaccgcttgg cgtcgccacc tgtagtcggt
      121 accacgtaaa cgtcggtgtt acccgcaacg gttgcgctga aagcaatctt ggatccatcg
      181 ggcgagaagt aaggatcaac ctcggctccc tgtgtcgagg ttaggcggcg ggcctctccg
      241 ccgctccgcg cgacgaccca caggtcgccg gcgtatgcga aagccacaag atcacggctc
      301 actgatggat gacggagcat tcgcgttccc tgggcgaagg cagtggacga gatcgcaaga
      361 cataacacta aacacaaggt catttttcgt gtgttcataa ggagcctcat aagaatgtca
      421 tttgaaaagt acataaccct aaaggtaggc cagagcgact caagtaacga atggtcatgc
      481 ccacattcat ttgatcttgt cgaataccac gttcccgatc ggatacttct gccaatcctt
      541 gtagtcaaaa tgccaccatt ccgcttcgta tacggtgaag ccctcagctt ccatcgcggc
      601 acgcagtagg tcgcggtgcc aacgctgcag tgaagtgccg ccgggatagt cggggtatga
      661 gcgatcagtg gtctcgtcat aagtgccaac catctcaaca ggttttccgc tcgccaggtc
      721 atagagagtc agatcaacag ccgcgccgcg gttgtggcgc gaccctcgtg atgggtcagc
      781 cacgaaaatt ttcttgtctt ccggcgtcgc atcccagaac actttggtca cataccaggg
      841 ccggtaagcg tcgtggataa gcaatccgta gcctttctgt tttagcttct gctgtgcgcg
      901 gacaagcgct tcagcggcgg gacgttgcag gaaggcccgc gcctcagagt aaaagacact
      961 ggccagaaag ttattgggcg tcgcgtagcg aatctccaat ttgattgttg gatcaagacg
     1021 attaagttca accagctccg cctcgcgaaa ctgtccagcc tcttttggcg gttgcgcaac
     1081 cagcgcttcc ttcaacaact cgctgatagg tctgagtggc ttgatgcgaa gttgcgcggc
     1141 accttcctcc ggccccacgt tgcgacgctt gaagacaacg ctggccgcct ccacttgagt
     1201 cgcgcgtcca gtttggtcac gagtgaagat taatcgctcg ccgtcataga gaccccggtc
     1261 cggaaacttg aagacgttct ccgagacttg ctcaagcggg tcgaattcga accattcaat
     1321 caaggcccaa agctttccgt ccttctcgaa gatgtaaagg atgtcgtgat cccagccgta
     1381 ctcgccgatg agtccgcgcc agcgaaccgt tgcaggcgga ggtttcggaa cttcgactcg
     1441 cttaaaggtt tcatcaccga tcaccagcgc attgtcgcga accaggattt tctcgccgta
     1501 cgcgagcttg tcatcgacga tgagcgcgtc gccaagtgat cggagtcgca cttgctctcc
     1561 cccgtcgctg ctaagcttag aaagtttgcc ggcgctctct atcaactcaa cactgttgga
     1621 gccgtttaca tagcgtcctg caattcggcg cgcaagctga ggatcgacgg gcccggatgt
     1681 ttcaggctgg gggatgggtt tgccctggcg gacggcaagc atagttgtca aagcgtagtc
     1741 ggccatgcga ttcgtcaccg cgttcgcaga gtccttggtc gttaccacga ctacgccaag
     1801 cttgtcatcg ggcatggcct tcagtgttgt tgcaaagcca tagatggcac cgccatggcc
     1861 gaccgtgcga cgaccttcaa gctcgcgcac tccaaaacca atgccgtaac ctgttttttg
     1921 tcccggcggt gcaaactgtg gcgtccacat ctggtcgatc gttgacgcct tgagcatctg
     1981 tccttgcgca cctcgcccac cggctagcag cgcgctcatg aaccgtccca tgtccgttac
     2041 cgtcgtgtac atgctgccgg cggggctc
//
LOCUS       DASUEX010000169         3914 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_488527, whole genome shotgun sequence.
ACCESSION   DASUEX010000169 DASUEX010000000
VERSION     DASUEX010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3914
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488527"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1965
                     /locus_tag="VFV61_02790"
     CDS             <1..1965
                     /locus_tag="VFV61_02790"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX4899428.1"
                     /translation="FIVMELLDGESLADRIRRGPIDLESLLTLGVQIADALESAHSKG
                     IVHRDLKPANIFVTSRGQAKILDFGVAKFDRHNAPDASSVVETIRKDQLTSAGTTIGT
                     VAYMSPEQARGEITDARTDLFSIGTVLYQMATGFLPFQGETSAVVFDALLNRDPAPLT
                     QYNATLPPELNRIIGQALEKDRDMRYQSATDLKTALKRLKRDLSSGQHSTTASGTRRA
                     AAPVSDHSIAVLYFENLSGMKEDEYLRDGITEDITTELSKIQGLKTFSRAMVLSYRDK
                     SVTAGQVGKELGASYVLAGSLRRAGERLRINAQLVDAATDFPLWSERYDREMKDVFEL
                     QDEIAQKIAAALRITLSPQEQQALSAKPTENLQAYDLYLRGRNYARRVGRQDLMFALQ
                     MYENAVALDPDFALAHAGLANVCAQYYYHFERQQQWIDRAIAATQKAISKGHDAPEIQ
                     LAEAWVIFAEGRYDECVVKIRAALSRNPDVDGGYYLLGRALFSAGRYQEVVDMMEEAL
                     AHAGENYNTIIPIHNSLGALGKKDALKNFSHREIAVYSAHVKKVPEDARARVLLASNY
                     AMNGRMDEAKREVDMAMILRPDDSMILYNAACLFCAMNNVPDALNAIRKAWESGYRDA
                     VWTRQDPDLELLHGNEEFEKLYPAASAKSA"
     gene            complement(2135..2833)
                     /locus_tag="VFV61_02795"
     CDS             complement(2135..2833)
                     /locus_tag="VFV61_02795"
                     /inference="COORDINATES: protein motif:HMM:NF012976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899429.1"
                     /translation="MFDSRRTFLKQSGIGLATIGASGLLLRSDAQPTQQVNRDQSDRV
                     LIQLAASRKDETPASVELKRVTPFPYGPFYRTGAPFRGKLSVPGEPGMPFVLSGRVWA
                     FDTKRPLSGVVLDFWHVDMNEKYSNGTTDLRNRGRLISSETGHYELESIRPIPYRPNP
                     QGAPEFWRCAHFHLLALYPGYQPLITEIHFQGDAKKSDPMYRVENAITVEERKVNGTT
                     FQTGVFDVVLLPEG"
     gene            complement(2995..3492)
                     /locus_tag="VFV61_02800"
     CDS             complement(2995..3492)
                     /locus_tag="VFV61_02800"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX4899430.1"
                     /translation="MSINPQPTLEIRTAVADDAPAIASVLYESFVEYESSYTPEGFAA
                     TTPTADQIRRRMIEGPTWVVLRDETIVGTVAAVAQGESLYVRGMAVLPAARGQKIGEL
                     LLAHIESYAAARGFQRLHLSTTPFLNAAIRLYERFGFQRNTDAPHDLFGTPIFTMAKE
                     LSSDA"
     gene            complement(3615..>3914)
                     /locus_tag="VFV61_02805"
     CDS             complement(3615..>3914)
                     /locus_tag="VFV61_02805"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX4899431.1"
                     /translation="LAQNSASDNPNRLRALILVTDGEDRGSRRRLDELLALLREKKIR
                     VYVVGLVTQLEKKSTARSTSFLVTLANETGGRAFFPKTVPELKDTANEMLGIIRN"
BASE COUNT          885 a         1069 c         1097 g          863 t
ORIGIN      
        1 ttcatcgtca tggagttgct ggacggcgag tcgctcgctg atcgcatccg gcgcgggcct
       61 atagacctcg agtcgctatt aactctaggc gtgcaaatcg cagatgcgct cgaatcggct
      121 cactccaaag gaatagtgca tcgtgacttg aagccggcaa acatcttcgt cacttcgcgc
      181 gggcaggcaa agattctcga cttcggtgtc gccaagttcg atcggcacaa tgcaccggat
      241 gcttcttcgg tggtggagac gatacgcaaa gaccagttga cctcagctgg aacgacgatt
      301 ggcaccgttg cttacatgtc gcccgaacag gcgaggggtg agatcaccga cgcccgcact
      361 gacctttttt ctatcggaac cgttctctat caaatggcga ctgggtttct tcctttccag
      421 ggagagacat cggcagtcgt gttcgatgct ctcctcaatc gcgatccagc accgttgacc
      481 caatacaatg cgacgttgcc gccggagctc aatcgcatca tcggccaggc attggagaag
      541 gaccgagata tgcgctatca gagcgcgact gatctcaaga ccgctctcaa acggttgaag
      601 cgcgatctca gttcgggaca acactccacc actgccagcg gcacccggcg cgctgcggca
      661 cccgtgagtg atcactccat cgccgttctc tacttcgaga atcttagtgg catgaaggaa
      721 gacgagtacc tgcgcgacgg catcaccgaa gatatcacca ccgagctctc aaaaattcag
      781 ggtttgaaga ctttctcacg cgcgatggtg ctttcttatc gcgacaagtc ggtaacggcg
      841 ggacaggtcg gaaaagaact aggcgcgtcg tacgtgctcg cgggtagttt gcgtcgtgcc
      901 ggagaacgct tgcgtatcaa tgctcagctc gtcgacgcgg caacagattt tccgctctgg
      961 tcggaacggt acgaccgcga gatgaaagac gtcttcgaac tgcaggatga gatcgcgcag
     1021 aagatcgccg ccgcactgcg catcacgctt tcaccgcaag agcagcaggc gctctcggcg
     1081 aaaccgactg agaacctgca ggcttatgat ctctacctgc gcggcaggaa ctatgcgcgg
     1141 cgtgtgggcc gtcaggatct gatgtttgct ttgcaaatgt atgagaacgc ggtcgcactt
     1201 gatccggact tcgcgctcgc acatgcgggg ctggcgaatg tctgcgctca gtactactac
     1261 catttcgagc ggcagcagca atggatcgat cgggccatcg cggcgacgca gaaagcgatt
     1321 tcgaagggtc acgacgcgcc ggagattcaa cttgccgagg cctgggtgat ttttgccgaa
     1381 ggccgttatg acgagtgcgt ggtaaagatt cgcgccgcgt taagccggaa tcctgatgtt
     1441 gatggtggtt attacctgct gggacgcgcg cttttctcag ccggacgata ccaggaagtc
     1501 gtggacatga tggaagaggc cctcgcgcac gcgggcgaga actacaacac aattattccg
     1561 attcacaatt cccttggggc gttgggaaag aaagatgcgc tgaagaattt cagccatcgt
     1621 gagatagctg tgtatagcgc gcatgtgaag aaggttcctg aggacgcacg cgcccgcgtc
     1681 ttgttggcga gtaactacgc catgaacggc agaatggatg aggccaagcg ggaagtggac
     1741 atggcgatga ttctgcgccc cgacgattcg atgattctct acaacgccgc gtgcctcttt
     1801 tgcgcgatga acaatgttcc cgatgcgttg aatgcgatca ggaaggcgtg ggagtcgggt
     1861 tatcgcgatg cggtgtggac gcgacaggat cctgatctgg agttgctgca cgggaatgag
     1921 gagtttgaga agctgtatcc ggcagcgagc gcaaaaagcg cctgacgcga ttgcgattgc
     1981 cgcggccctg gggtgtttca ggtctcaagt ctcaggtctc aggtctcaag tctaggcgtc
     2041 tgtctcaatg cactttccaa atatcttcag ggccggagtt tcgagcgtga gtcctgagac
     2101 ttgagacctg agacccctac tgtcggcaat cgcctcatcc ctctggcagc agcacaacat
     2161 caaacacgcc ggtctgaaaa gttgtgccgt ttactttcct ttcctcgacc gtgatcgcgt
     2221 tttcaactcg gtacattgga tcgctcttct tcgcatcgcc ctgaaagtga atctcggtga
     2281 tgagtggctg ataacccgga taaagcgcta gcaggtggaa gtgagcgcag cgccagaatt
     2341 ccggggcgcc ctgagggttt ggtcgatagg gaatcggacg aatgctttcc agttcatagt
     2401 gaccagtttc cgatgaaatc aatcggccac gattgcgcaa gtcagtcgtg ccatttgaat
     2461 acttctcgtt catgtcgacg tgccaaaaat ccagaaccac acccgacaaa ggccgcttag
     2521 tgtcgaaggc ccacacgcga ccagaaagca caaacggcat ccccggctct ccgggcacac
     2581 tgagcttgcc tcggaaaggt gcgccggttc tgtagaacgg accgtaggga aaaggtgtga
     2641 cccttttcag ttcgacactt gcaggcgtct cgtctttcct gcttgcggcc agctgaatca
     2701 gcacacgatc cgactgatcg cggtttacct gctgagtggg ttgcgcgtca ctcctcagta
     2761 gcaagcccga agcgccaatg gtcgccagtc caattcctga ttgcttcagg aacgttcgtc
     2821 gtgaatcaaa catgatgctt taccttcagt tcagaatgct ggttctaagg aagagacact
     2881 gctggtcagg atcttgtggc gaaaatatct gcggatcagt cgtcgctacg cgacgatgcc
     2941 gtaggaggca tggccttgcc cggcgttgaa acgccgggct aaattccgcc gccgctacgc
     3001 gtcggagctt aattcctttg ccatcgtaaa gattggagtt ccaaacagat catgcggcgc
     3061 atcagtgttt cgctggaaac cgaaacgctc atagagccga atggcggcat tcagaaaggg
     3121 cgtagtgctg agatgtaaac gctggaagcc acgagcagcc gcatagctct caatatgcgc
     3181 caatagtagt tctcctatct tttgtcccct tgccgcaggc agcacagcca tccctcgtac
     3241 atagagtgat tcaccttgcg ccacggctgc tacagtgccg acaatagtct cgtcccgcaa
     3301 gaccacccac gtcggtccct caatcatacg gcgccgaatc tggtcggcgg tcggagtggt
     3361 ggcagcgaat ccttccggag tgtatgagga ttcatattcg acgaacgact cgtacaaaac
     3421 ggaagcgatt gcaggagcat cgtccgcgac agccgttcgt atctcaaggg taggttgggg
     3481 attgatgctc atcgcagatg atgtgagtac cacagcgccc agcgtgagag ccgaaccact
     3541 aagcaacact aaaccacacc aagcttgagg ctccaacctc ttcgatcgcc aatcgggaat
     3601 cgaaggctcg tcagttaatt acggattatt cccaacattt cgttggcagt gtctttaagt
     3661 tcggggacgg ttttggggaa gaacgctcgc cccccagtct cattcgccag cgttaccagg
     3721 aaggaagtcg atcttgctgt cgactttttc tcaagttgtg tgaccagccc aacaacataa
     3781 acccggatct tcttttcacg cagcaacgcc aaaagttcat ccagtctccg acgactgcct
     3841 ctatcctcac catccgttac cagaatgagt gcacgaaggc ggttagggtt atcactcgcg
     3901 ctgttttgag caag
//
LOCUS       DASUEX010000170         3379 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_949898, whole genome shotgun sequence.
ACCESSION   DASUEX010000170 DASUEX010000000
VERSION     DASUEX010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3379
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_949898"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            283..1434
                     /locus_tag="VFV61_02810"
     CDS             283..1434
                     /locus_tag="VFV61_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899432.1"
                     /translation="MNFKVLRIITCALMLILAVTSPSIAPSPGHAAPAQSPDITRTFD
                     FRDGTLGWQPDFVGYTQYNQDIYELLAELRALPPELSVSGTGFYLQGHNRCDCLVMFL
                     KRRLGAQDGVVAGQRYRASFTVRLASNAQSGCIGIGGSPGESVRLLVGASQIEPVTFV
                     GPYDTRRLNLESTVATLAGNIANGQPCNLNLNPYISIERMQQHVSEVTADSAGNLWLF
                     VGTGSGFEGRTTLYYQGIDVVLTPVGTPEANPVNPPTLLTEENTQNAVAINAQTFTRD
                     PFPFYTTGFFSSDGRTYIMFFAANTQLMEGESLSTVTVLAEDERGIKFQLPVDSVLRV
                     PNFDWLTQIVVRLPNELIGARNVRVSFNLRGASSNTAALKVISDANRSQ"
     gene            1487..2443
                     /locus_tag="VFV61_02815"
     CDS             1487..2443
                     /locus_tag="VFV61_02815"
                     /inference="COORDINATES: protein motif:HMM:NF006159.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX4899433.1"
                     /translation="MKQRLKGKVALVAGATRGAGRGIAIELGAAGATVYCTGRSTHAQ
                     PSEIKRPETIEETAELVTQAALESGAEGRGERQEGGRGIGVQVDHLDPAQVEALIKRI
                     ESERGQLDILVNDIWGGEHLTEWYKPVWEHSLEKGLHQLRLAIDTHIITSHFALPLLI
                     KKPGGVVVELTDGTVEYNKERYRLSLFYDLAKTSINRIAWAQAQELEKHGCTAVSLTP
                     GWLRSEQMLDNYEVKEANWRDAIAKQPHFAIAETPRYVGRAVAHLAADPNLSRWNGQS
                     LSSGQLAKVYGFTDLDGSQPDAWRYVVEVQEAGKPTDVTGYR"
     gene            2465..>3379
                     /locus_tag="VFV61_02820"
     CDS             2465..>3379
                     /locus_tag="VFV61_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899434.1"
                     /translation="MIAGGCARSNQSNGRGITVVGVRSLNSAEELKRYVDFGQEVYRH
                     IPQWVPPDSHHLIELLSGNGGFGPDTQIQPFWVEADGRVLATITAVTNEAYNQRWNEQ
                     LGHLVFFEALPEQNEAVEMLMRAVDDWFRARACQSVRLSLLPGMQLGITIDGYDKVPT
                     VFHAYNPPYYHSYIKNGGFRTECGVVQYQVTFTPELAERYRGMVKRAEQADVSLRTWD
                     FERMESENETFTTLFNETFANHFGFMALPPAVMSGLTVGLKDFLIPDFTLFAETEGET
                     VGFVYSLPDLNQVFHPLKGKDLEENLAEL"
BASE COUNT          822 a          852 c          936 g          769 t
ORIGIN      
        1 tgaccggcaa gttgtcctgg gcgttggctg caacagtcag cattgccagt actacaataa
       61 ggtttcttgc tctcattttt ttgcgctcca aacctggatc caattatatt gaggccagac
      121 aacagaaaaa caggtcgtgt tctggtcggc ctttgtttgg gcaaaacccg aacttaggat
      181 ggcatccgac gtttgagtca gcaagcaatc tggcaatcta aaaacaccgc gatcatacac
      241 aagatggcat tgagcctttc atggcagttg tgaggtaaag tcatgaactt caaagtgttg
      301 cgaataatta cttgcgcatt aatgttaatt ctcgctgtga cctcgccttc tattgctccc
      361 agcccaggtc acgcggcgcc agcacaaagt cctgacatta cgcgaacctt cgatttccgg
      421 gatgggacgc tcggatggca gcccgatttt gttggttaca ctcaatataa tcaggatatt
      481 tacgagttac ttgcagaact ccgagcttta ccgccagagt tgagcgtgag cggcactggg
      541 ttttacctgc aaggtcacaa tcgctgcgac tgtctggtca tgtttctgaa acgccggctt
      601 ggtgctcaag acggcgttgt tgctggccag cgatatcgtg ccagcttcac cgtcaggttg
      661 gcctcgaacg cccagagcgg ttgcattggg attggaggtt caccaggcga gtcagttcgc
      721 ttgctggtcg gcgcctcgca aattgaaccc gttactttcg tgggccccta cgacacgaga
      781 cgcctgaacc tcgaatcaac cgttgccaca ctagcgggca acatcgccaa cggccagccc
      841 tgcaacctta atttaaaccc atatatttcg atcgagcgaa tgcagcagca cgtaagtgaa
      901 gtcacggcgg atagcgcagg caacctatgg ctgtttgtag gaacggggtc cggatttgag
      961 ggtcggacaa cactctacta tcagggaata gacgtggtct taacaccggt gggaacgccg
     1021 gaagcaaatc ccgtgaaccc tcccacgttg ctaaccgaag aaaacacgca gaatgccgtt
     1081 gcgattaacg cacaaacgtt cacgcgcgat ccttttcctt tctacaccac cggttttttc
     1141 agcagcgatg ggcgtaccta cattatgttc ttcgctgcca atacacagct gatggaaggg
     1201 gaaagcttat ctacggtgac ggtactagcc gaggatgaga ggggaatcaa attccaactg
     1261 ccagtcgact cggtgctgcg agtgccaaat ttcgactggc taacgcagat cgttgttcgt
     1321 ttgcctaacg aattgatagg ggccagaaat gtacgcgtga gtttcaacct ccgcggcgca
     1381 agtagtaaca ctgctgctct caaagtcatc tctgatgcga atcgttctca atgaactggc
     1441 gtgctcctca ttcgggcaaa atcggcattc caaaaatcgg ctcaaaatga agcagagact
     1501 taaaggcaaa gttgctctgg ttgcaggggc gacgcgtggt gccgggcgcg gcatcgcgat
     1561 cgaactgggg gcggcaggag cgactgtgta ctgcactggc cgcagcacgc acgcgcaacc
     1621 ttccgaaatc aagcgtcccg agacaattga agagacggcg gagttagtga cgcaagctgc
     1681 cttggaatca ggagcagaag gcagaggcga aaggcaggaa ggtgggcgag gcattggcgt
     1741 ccaggttgat cacttggatc cggcgcaagt agaagcactc attaaaagga tcgaaagcga
     1801 gcggggccag ctcgacatcc tggttaacga tatttggggc ggggagcatt taacggaatg
     1861 gtacaagccg gtttgggaac attcactgga aaaaggcctg caccaactgc ggctcgctat
     1921 cgatacacac atcatcacca gtcacttcgc cttaccactc ttaatcaaaa agccgggtgg
     1981 cgtggtcgtt gaactaaccg atggcacggt cgaatacaac aaagaaaggt atcgcttgtc
     2041 gctgttctat gatcttgcaa agacgtcgat aaatcgaatc gcctgggctc aggcgcaaga
     2101 gttagagaaa catggttgca ctgctgtttc cttaactccg ggatggctgc gttcagaaca
     2161 gatgctggat aactacgaag tgaaggaagc gaattggcgc gacgcaatcg ccaagcaacc
     2221 gcattttgcg attgcggaaa cacctagata tgtgggacgc gcggttgcgc acctggctgc
     2281 tgatcctaac ttgtcgcgct ggaatggcca gtcgctttcg agcggtcagc ttgccaaggt
     2341 ctatggcttt actgatcttg atggatcgca gcccgatgcc tggcgttatg tagttgaggt
     2401 tcaggaagcc ggcaaaccaa ccgatgtaac tgggtatagg tagaggtttc aggtctcagg
     2461 tctcatgatc gcgggcggat gtgcgcgatc caaccaatcg aatgggaggg gcattacagt
     2521 tgtgggtgtc agatcattaa actcagcgga agagctcaag cgttatgtag acttcggtca
     2581 ggaagtctat cgccacatcc cgcaatgggt gccacctgat tcgcatcatc tcattgaact
     2641 attaagtggc aatggcgggt ttggacccga cacccagatc cagcctttct gggttgaagc
     2701 tgatggccgc gtgctggcga cgattaccgc ggttacaaac gaagcctaca accagcgctg
     2761 gaatgaacag ctgggacatc tggttttctt cgaggcactg cctgagcaaa acgaagcagt
     2821 cgaaatgttg atgcgcgcgg tcgacgattg gtttcgcgcg cgcgcttgcc aatcggtgcg
     2881 cctgtcccta ttgcccggga tgcaattggg aataacaatc gacggctacg acaaagtccc
     2941 gactgttttt cacgcttata atcctcccta ctatcacagc tacattaaga atggtggctt
     3001 ccgaaccgaa tgtggcgtgg tgcagtatca ggttactttc acgcccgaac ttgccgaacg
     3061 ttaccgggga atggtgaagc gggccgagca ggcggacgtc tcgctgcgaa cctgggactt
     3121 cgagcgcatg gaatcggaga acgaaacctt caccacactc ttcaacgaaa cctttgcgaa
     3181 ccacttcggt ttcatggcat tgccgccagc ggtgatgagc gggcttactg ttgggttaaa
     3241 agacttcttg attccggact tcacactctt cgcagagacg gagggagaga cggtgggatt
     3301 tgtttattcg ctgcccgatc tgaaccaggt tttccatccg ctgaagggca aagacctgga
     3361 ggaaaacctt gccgagctt
//
LOCUS       DASUEX010000171         3148 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_364586, whole genome shotgun sequence.
ACCESSION   DASUEX010000171 DASUEX010000000
VERSION     DASUEX010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3148
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364586"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(199..1905)
                     /locus_tag="VFV61_02825"
     CDS             complement(199..1905)
                     /locus_tag="VFV61_02825"
                     /inference="COORDINATES: protein motif:HMM:NF014589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HEX4899435.1"
                     /translation="MYILGLTTLGDSAATLIHDGEIVAAAEEERFSRRKHHSGFPYNA
                     MQYCLEEAGIKVADLAHIALYWKPWVLRHKAMQALKSAVISREMFKARVDRGVAQVSE
                     SYLGMFRHPQLIRKHFGPGNFRFHFLEHHQCHAASAFFVSGFDRAAIMTWDGTGEDTT
                     TLFSQGQDRNIKVLKRIKLPHSLGQFYSAVTNFIGFDMFGGDEWKVMGLAAYCKPEYY
                     DFLARKVLTLNGNHDFHLNIRVLDHHLAKHYQFGEEISRELGPPRRPDEELTQRHWNI
                     AASAQKVLEDTALHLLKGLHERTGEENLCMAGGVAFNSVMNGRIMTESPFKNYFVQPA
                     AGDAGCSLGAALLVHHQKLQQPRKFVMEHAYYGPGFTSASCKAALDDAGLKFETLPDE
                     QMLPRVAQMMAAGAIIGWFQGRMEFGPRALGNRSFIADPRRADMRELLNRKVKLREWF
                     RPLAPSMKEEVSADMFGHPHHDPFMITVLHVTPEQRERFPAVVHVDGTARPQTVSRKT
                     NPRYWSLITEFEKLTGCPMVLNTSFNIQEPIVCRPEDAIKTFQSASFDALVLENHLVL
                     RQ"
     gene            complement(1905..3077)
                     /locus_tag="VFV61_02830"
     CDS             complement(1905..3077)
                     /locus_tag="VFV61_02830"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX4899436.1"
                     /translation="MYLRLLDFLRCPTCQARLSCIALEQQQQDEGGEIVSGLLQCAAA
                     SHWFPIVHGIPRMLPDAILEHWPRIQELIPEPVPTLLEPLITSLKSQKAVGYHQATRD
                     NFSHEWALHDLGGRTWGISLSDRVQWFFLDALNIQKEDLVGRLMLDAGCGNGSQSVAY
                     TALGVEVIAIDLSSGLEKGYAYRRLHSGARPEHVHFVQGNLQHPPLAPNTFDIIHSAG
                     VLHHTPDTLTTFRALRPLLRPGGTFYVWLYKYERLVTPIVNSIRAVSTKISPASFARI
                     ADFMALPFIGFCKTVNALGIRDYSNMNKREAALALLDIFGAPYAYYHSYDEVCGWYKS
                     EGFGEVWPCNDGRRGFGVCGRDGLAMVSRPSETREQQSARAASQSPNAPVMHRERA"
BASE COUNT          751 a          867 c          838 g          692 t
ORIGIN      
        1 ggagagcatt gtcgacatgg gtggataaac attctcatcc gagccggtca ctgaaaagcc
       61 acgcgcttga agcatacccg cgagcgaggc catcgcagtg ccgcaaattc caatcaggtg
      121 atagtgcatc gatgattgaa cgctaaggaa ggatcagtta gagagcctca cttcagacgg
      181 ggtggaccct ctgtgtggtt actgtcttaa gaccagatgg ttttccaaaa ccagagcgtc
      241 aaaacttgcg ctctgaaaag ttttgatcgc gtcttcggga cgacaaacga tcggctcctg
      301 aatgttgaat gaagtattta gaaccatcgg acaacccgtc agcttttcaa attcagtgat
      361 tagtgaccag taacgcgggt tcgtcttgcg gctaacggtt tgcggtcgtg ctgtgccatc
      421 aacgtgcacc actgcgggga accgttcccg ctgttctgga gttacatgca gcaccgtaat
      481 catgaacggg tcgtgatgtg gatggccaaa catgtccgcg ctgacttctt ctttcatcga
      541 cggggcgagg ggacgaaacc attcgcgcag ttttactttc ctgtttagca gttcgcgcat
      601 atcagcccgg cgtggatccg cgatgaaact gcgattaccc aaagcgcggg gcccaaactc
      661 catccgcccc tgaaaccagc cgatgattgc accagcagcc atcatttgcg ccacgcgtgg
      721 cagcatctgt tcgtccggca gagtctcaaa cttcaaaccg gcatcatcaa gtgcggcctt
      781 acaggatgcg gaagtgaaac ccggcccgta ataggcatgt tccatcacga atttccgcgg
      841 ctgctgtagc ttttgatgat gaactagtaa ggccgcgccc agcgagcatc ccgcatcacc
      901 ggctgcaggt tgcacgaagt agttcttgaa cggagactcc gtcatgatgc ggccgttcat
      961 cacggaattg aaggcgacgc cgccggccat gcagaggttt tcttcaccag tgcgttcgtg
     1021 caatcccttg agcaagtgca gtgcggtgtc ttccaaaacc ttttgggcac tcgccgcaat
     1081 gttccagtgc cgttgagtca gctcctcgtc gggccgccgc gggggcccaa gctccctgct
     1141 aatctcttcc ccgaattggt agtgcttcgc caaatgatga tcgagtacgc gaatgttgag
     1201 gtggaagtcg tgattgccgt taagcgtgag caccttccgg gccaggaagt cgtagtactc
     1261 gggtttgcaa taagcagcaa gccccatcac tttccattca tcaccgccaa acatgtcgaa
     1321 gccgataaag ttggttaccg cagaataaaa ctgccctagc gagtggggaa gcttaattcg
     1381 cttcagcact tttatgttgc gatcctggcc ctgcgaaaag agtgtcgtcg tgtcttcccc
     1441 agtgccgtcc caggtcatga tcgccgcgcg atcgaatccc gagacaaaaa acgcgctggc
     1501 ggcgtgacac tggtgatgtt caagaaagtg gaagcgaaag ttgcccggcc caaagtgttt
     1561 acgaatcaat tggggatggc gaaacatgcc cagatagctc tcgctgacct gagctacgcc
     1621 gcggtccacg cgcgctttaa acatctcgcg cgaaatcacc gccgacttca gcgcctgcat
     1681 agctttgtga cgcaagaccc atggtttcca gtagagagcg atgtgggcga ggtcggcgac
     1741 ttttatgcct gcttcttcga ggcagtattg catcgcgttg taaggaaaac cgctgtgatg
     1801 cttgcgacgt gaaaaacgct cctcctccgc ggccgcgaca atttcgccgt cgtgaatcaa
     1861 agtggctgcc gaatcaccga gcgtagttaa accgaggata tacattaagc tctctctcta
     1921 tgcatgactg gcgcgttcgg cgattgagac gcagcgcgag cagattgttg ctctctggtt
     1981 tcagacgggc gagacaccat tgccaaacca tctcttccgc aaacgccgaa gccccggcgc
     2041 ccgtcattgc aaggccagac ttcaccaaag ccttcggact tataccaacc gcaaacctcg
     2101 tcgtacgaat gatagtacgc gtacggtgct ccaaagatgt ccagcaacgc cagcgccgcc
     2161 tcacgcttgt tcatatttga atagtcgcga atgccaagcg cattaacggt tttgcagaaa
     2221 ccgataaatg gcaaagccat gaaatcggca atgcgcgcaa aacttgccgg cgagatctta
     2281 gtcgaaaccg cccggatcga attcacgatc ggtgtgacta gcctttcgta cttatataac
     2341 cagacataga acgtgccacc gggccgcaag aggggacgca aggcgcgaaa cgttgtaagc
     2401 gtatcaggcg tgtgatgcag cacgcccgct gaatgaatga tatcgaacgt gtttggcgcc
     2461 agcggcggat gctgcaaatt gccctgcaca aagtggacgt gttcggggcg agcgccagaa
     2521 tgcaagcggc gataagcgta tcccttttcc aatccggagc tgagatcaat tgcgattact
     2581 tcaacaccaa gggcagtgta agctaccgac tggctgccat ttccgcagcc ggcatcgagc
     2641 attaatcttc cgactagatc ctctttttga atattcagtg catcaagaaa aaaccactgg
     2701 acgcgatcag aaagcgaaat cccccaggtc ctgccgccaa ggtcgtgaag agcccattca
     2761 tgactgaagt tatcgcgggt ggcttgatga tagccaactg ccttttgcga tttcagactg
     2821 gtaatcaacg gctcgagcaa agtcggaaca ggctccggga ttagctcttg gatcctgggc
     2881 caatgttcca gaatcgcatc cggaagcatc cgtggaatcc cgtgcacgat cggaaaccaa
     2941 tgcgaggccg ctgcacattg cagcagtccg cttacgatct cgccgccttc gtcctgctgc
     3001 tgctgctcca aggcaatgca gcttaatctc gcctggcagg ttgggcagcg taaaaaatcc
     3061 agcaatctta gatacatcag cgtctttgct aaatcagact cctcgcaaca aaagcaggag
     3121 ctggggcgat tccaattcca cggtggcc
//
LOCUS       DASUEX010000172         2254 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1523612, whole genome shotgun sequence.
ACCESSION   DASUEX010000172 DASUEX010000000
VERSION     DASUEX010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1523612"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="VFV61_02835"
     CDS             complement(<1..361)
                     /locus_tag="VFV61_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899437.1"
                     /translation="MKIFFNNAGRLRSGWRVLIFAGLFVAVLFALSTLLRFAYALLIN
                     LAPSLTPNSYFENVIYRFLFTAAALLAGYICTRGLEGLPWRALGLPLHRNWLRQLLIG
                     SMIGVGSLALAALIATAG"
     gene            502..>2254
                     /locus_tag="VFV61_02840"
     CDS             502..>2254
                     /locus_tag="VFV61_02840"
                     /inference="COORDINATES: protein motif:HMM:NF039586.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEX4899438.1"
                     /translation="MSDLHENPASSKPNRLSQRGGLFLWVLGFTVLLMLAVIVSQQTW
                     LWTVVQPDTASDTLVLYALSTLNFVAFVVFSFIFVRNLVKLRRERRERQLGSKIKTRL
                     LVYFIAISFLPITAMAVFSYLFLNRSLEKWFSPFPEGVVQEAREVRRQSLERQNSSLR
                     ETAALLAPMLNRQSEVERQQTLDQITANGQLAAIEIVNSDGTVATRSRAELSPGHLTE
                     LEALLETARSAKTDIGERLADGKAFDVIAAPLGNDQRLLLAPARRADTDLNDTIADSQ
                     SEYQKLVKRQRRVRLLGVSTLGLMTLMLLFVSSWVAIYLARGIATPIKALAEASDEIA
                     KGNLRHRVDTTAEDELALLAHSFNQMTAQLEENRRRLDAGSAELRDKNLALEERRNYI
                     ETVLESLSTGVISLDEQDHVTTINAAAARMLRLSPSPASDTTFSELLSTEDHTTVAQL
                     LSRARRAGQASEQAQLVGCNPTDAGPLPVALTATALRPTPGEKRGVVLVIEDLSELLA
                     AQRAAAWSEVARRMAHEIKNPLTPIQLSAERIAKSYQKLRANGHEQGTNGLDEPVDEL
                     RVGAVVDECTETITREVA"
BASE COUNT          542 a          587 c          651 g          474 t
ORIGIN      
        1 cgccggcggt agcgattagg gctgcaagcg cgagtgaacc gactcctatc atcgatccaa
       61 ttaatagctg gcgcagccag ttccgatgca gcgggaggcc caaagcacgc cagggaagac
      121 cttcaagacc gcgggtgcaa atgtaaccgg ccaggagagc agcggccgta aagaggaaac
      181 gataaatgac attctcgaaa taggagtttg gtgttagcga gggcgcaagg ttgattaaca
      241 gtgcgtaagc gaaccggagc agcgtgctca gggcgaaaag tacggcaaca aaaagaccgg
      301 caaaaatgag cactcgccag ccactccgga gccttccggc attattgaag aagattttca
      361 tgagatttcg aattagagac tgcgaggcat tctagtccat gagggccagg atttcatcct
      421 gacccaagca cacgctcact ctcttcaaac cgaaggtaag tccgagcaga taccgtgtgt
      481 taaactttga acttcatttc aatgagtgat ctgcacgaaa atcctgcttc atccaagcca
      541 aatcgactga gccagcgtgg aggtctattc ctctgggtac tcggatttac ggtgctgcta
      601 atgttggcgg tgatcgtgtc gcaacagacg tggctttgga ctgtcgtgca accggataca
      661 gcctcggata cgctggtctt atatgcactc tccacgttga attttgtggc ctttgtggtc
      721 ttttcattca tcttcgtccg caacctggta aagctgcggc gcgaacgtcg cgagcggcaa
      781 ctcggttcga agatcaaaac acggttgctg gtctacttta ttgccatcag ctttttgccc
      841 atcacggcga tggccgtttt ctcatatctc ttccttaacc gctcactgga gaaatggttt
      901 agtccttttc cggaaggtgt ggtgcaggag gcgcgggaag tgcggcgaca gtctttggaa
      961 cggcagaata gcagcctacg ggaaacggca gcactgcttg cgccgatgct gaaccgccaa
     1021 tcggaagttg aaaggcagca gacgctcgat cagatcacgg cgaatgggca acttgccgcg
     1081 attgaaatcg tgaattcgga cggcacggtc gcgacaagga gcagggccga actgagtccg
     1141 ggccacctga ccgaattgga ggctctgctg gaaacggcgc gcagcgcaaa aaccgatatc
     1201 ggtgaaaggc tggcggacgg aaaagctttt gatgtgattg ccgcgccact cgggaatgat
     1261 cagcgactac tgcttgcacc cgcgcgccga gcagacactg acttgaatga cactattgcg
     1321 gattcacaga gcgagtatca aaagctggtg aagaggcaga ggcgtgtgcg gctgctcgga
     1381 gtttcaactc tcgggttgat gactttaatg ctgctttttg tgtcgagctg ggtagcaatc
     1441 tatctggcac gtggtattgc cactccaatc aaagctctgg ccgaggcgtc ggatgaaatt
     1501 gcgaagggga atcttcgcca tcgcgtagac acgacggctg aggatgagct ggcactgctg
     1561 gcccattcat tcaatcagat gacagcgcag ctggaagaaa atcgccggcg ccttgatgcc
     1621 ggctctgctg aattgcgcga caagaacctg gcgttggagg agcgtagaaa ctacatcgag
     1681 accgtattgg aatctctttc caccggcgtg atttcgctcg atgaacagga tcacgtcaca
     1741 accatcaatg ctgctgcagc acgcatgctt cgtttgtccc catctcctgc ttccgatacg
     1801 acgttcagcg agttactgag tacggaagat cacacaactg ttgcgcaact gttgagtcgg
     1861 gcgcgccgtg ccgggcaggc ttccgaacag gcccaattag ttgggtgcaa cccgaccgac
     1921 gccggtccac ttcctgtcgc cctaactgct acggctttac gcccgacgcc cggagaaaag
     1981 cgaggagtcg ttctcgtaat tgaagacctg tctgagttgt tagccgccca gcgcgccgcc
     2041 gcctggagcg aggtggccag acggatggcc catgagatca agaatccgct gacgccgatt
     2101 caactctcgg ccgaacggat tgccaagagc taccaaaaac tccgggcaaa tggccacgag
     2161 cagggaacca acggactgga tgaaccggtt gacgaactgc gtgttggcgc agtggttgat
     2221 gaatgcacag aaacgattac ccgcgaagtt gccg
//
LOCUS       DASUEX010000173         4891 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1278905, whole genome shotgun sequence.
ACCESSION   DASUEX010000173 DASUEX010000000
VERSION     DASUEX010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4891
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1278905"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..748
                     /locus_tag="VFV61_02845"
     CDS             <1..748
                     /locus_tag="VFV61_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899439.1"
                     /translation="EEIANLATTDLEELTSARAPLSEIGGHLEQLKTILDDIEAGANP
                     ALQNKEELQDALDRARSYVATAQASTDAAGGSDADIAKNNLQLLAVGFGAVAPAMLAE
                     VGKYVNELQSPLAAAGLANEGQRLLAAARELPQLQSKIKQGLKRVSVSEIERKANQDV
                     AYTARILDVTFRELNLLAISPVAMFDVARIDDRSGSGFGGLRYGLGGGLRLSVVSLDL
                     TAGYSWNPNRRSGEGRGAFVFSLDVSDLFR"
     gene            complement(927..1481)
                     /locus_tag="VFV61_02850"
     CDS             complement(927..1481)
                     /locus_tag="VFV61_02850"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX4899440.1"
                     /translation="MRKVKYFVANSLDNFIARPDGGVEWLFNDGTDYGMAEFFRSIDC
                     VLLGRKTYDFATGHGQRQRKSKKSKKKASVSWGMKSYVFSRTLKAEPDDDFTVVSENA
                     GAFVQKLKNEPGRDIWLMGGGVLAGSLLTAGVVDEIGLNIHPVLLGEGIPLFSGLTIQ
                     RDLELIESKAHQNGCVQVAYRVKS"
     gene            complement(1517..2053)
                     /locus_tag="VFV61_02855"
     CDS             complement(1517..2053)
                     /locus_tag="VFV61_02855"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX4899441.1"
                     /translation="MRTVTFGVANSLDNFIARADGAVDWLVWDDEVAAISREFWKTID
                     TVVMGRKTYEVAARMGSGAYPGVKNYVFSRTLKQTRSKKLEVISRNSAEFVRQLKQEA
                     GKGICVIGGGELASALFKAGLIDQVGLNIQPVMLGSGIPLFYAMTEQLDSELLEHKKL
                     KNGSVFIFYRVKKTESDA"
     gene            complement(2099..3169)
                     /locus_tag="VFV61_02860"
     CDS             complement(2099..3169)
                     /locus_tag="VFV61_02860"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX4899442.1"
                     /translation="MKLTYQVVLALVITPVLFSSPMKTPAEKAAAMDALFSDYAQPGA
                     PGASVIVIANDKVLFKKAYGLANLEAKIAATPNTNYRLASVTKQFTAMAIMILAERKQ
                     LSYDDSLASFFPGFPEYGKQVTVRHLLNHSSGLIAYEDVMDENATVPLTDQDVLELMK
                     RQDHTHFPPGSSYRYSNSGYVLLGLIVEKAAGMSFPEFLRRNIFAPLKMDGTVLYQRD
                     DRSDEAHRAYGYSKRGEVFERTDQSLTSSTRGDGTVYSSVADLYKWDRALRSSRLVKR
                     ATLQQAFTSGARVDEDTGYGFGWFIEKRRGLRTVWHSGNTIGCTQFIRRYLDRKFTII
                     VQANRNNALLAELVDKIEDIYF"
     gene            complement(3197..4705)
                     /locus_tag="VFV61_02865"
     CDS             complement(3197..4705)
                     /locus_tag="VFV61_02865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4899443.1"
                     /translation="MRKKTSGAWILAATILGSSMVFIDGTVVNVALPALQSNLNATVV
                     DVQWVVEAYALFLAALLLTGGSLGDHFGRRRVYIIGVFLFALASVWCGLAPNINQLII
                     ARAAQGIGGALLVPGSLAIISNAFAADERGRAIGTWSGFTAITAAVGPVLGGWLIEKV
                     SWRAVFFINAPFAIIVLVLVFLHVPESRDEESQGLDWWGATFATISLGAIVFGLIESS
                     RLGFSHPLILAALCVGVVTLVLFLFVEARKRNPMLPLTMFRSRNFSGANLLTLFLYTA
                     LSGALFFLPLNLIQVQGYSPTAAGAALLPFILLMFLLSRWSGGLIKRYGAKLPLIIGP
                     IIVAVGFVLFMLPGVGGSYWQTFFPAVVVLGLGMAISVAPLTTTVMSSVKENRAGVAS
                     GINNAVSRTAGLLAVAVFGLVMLQGFNSRLDERLNALQLAPQVRHELDQQRVRLADAE
                     PPAELDEATKTKLHQAIEESFVTGFRRVAFGSALLALLSAVSAWWLIAGKKF"
BASE COUNT         1194 a         1352 c         1308 g         1037 t
ORIGIN      
        1 tgaagagatt gcgaatctcg caactaccga tcttgaagag ctcacctcgg cgcgggcgcc
       61 gctcagcgaa atcggtggac atctcgagca gttgaaaaca attctggacg acatagaagc
      121 cggagctaat cctgctttgc aaaacaaaga ggagcttcag gacgcactcg atcgcgctcg
      181 ctcgtatgtc gctacagcgc aggcttctac cgacgcggct ggcggcagcg atgccgacat
      241 tgccaaaaac aatttgcaat tgttagcggt cgggtttggc gcagtggctc ccgcgatgct
      301 ggcagaggta ggcaagtacg ttaacgaatt gcagtcgcca ctggctgcgg ccggactcgc
      361 gaatgaggga cagaggttgt tggccgccgc gcgcgaactg ccgcaactgc aaagcaagat
      421 caaacagggt ctgaagcgtg tttcagtgtc tgaaattgaa cgcaaagcga atcaggatgt
      481 cgcgtacacc gcgcggatcc tcgatgtcac ttttcgtgaa ctcaacctgc tcgcgattag
      541 cccggtggca atgttcgacg tggcacgcat cgacgatcgc agtggttcag gttttggcgg
      601 gttgcgctac ggcctcggcg gcggcctgcg actcagcgtg gtgtcgttgg atttgacggc
      661 cggctattcg tggaatccga accggaggag tggcgaaggt cggggcgcat tcgtattctc
      721 actcgacgtc agcgatttgt ttcggtagat gctactcccg aaaggttacc gccaagccgc
      781 caagttacgc caagttcgcc aaattagttt gattcttgag agatcaaaca tctgtataag
      841 tgattttgga cgaatcttgc ttgaacggac atcagtcttg gcgaacttgg tgagacttgg
      901 tggcttggcg gtaatgccat acaaattcaa cttttcactc gataagcaac ttgaacacaa
      961 ccattctggt gcgcctttga ttcgataagc tcaagatcgc gctgaatcgt cagcccggaa
     1021 aacagaggga ttccctctcc caaaagcacc ggatgaatgt tgagaccaat ctcgtcaacc
     1081 acacctgccg tcaaaagact accagccaac acaccgccac ccatgagcca gatgtctctc
     1141 cccggttcat tcttcagttt ctgcacaaac gcgccggcat tctcagaaac gactgtgaaa
     1201 tcgtcatcgg gctcggcctt gagcgtgcga gaaaagacat agctcttcat gccccaactg
     1261 acactcgcct tttttttcga ctttttggac ttgcgctgtc gttggccgtg gccggttgcg
     1321 aagtcataag tctttcgccc cagcaacaca cagtcaatcg aacgaaagaa ttccgccatg
     1381 ccgtaatctg tcccgtcgtt aaataaccac tcaacgccgc cgtccgggcg cgcgatgaag
     1441 ttatcaagac tgttcgcgac gaaatatttt acttttcgca ttccggtaag ccgtaggggc
     1501 gtccgcctac aaattgttat gcgtcgcttt cggttttctt tacgcgatag aaaataaaca
     1561 cactgccatt cttgagcttc ttgtgctcaa gcaattccga gtcgagttgc tccgtcattg
     1621 cgtaaaagag tgggataccg gagccaagca tcactggctg aatattcaaa ccaacctggt
     1681 cgatcaagcc cgccttaaac aacgcgcttg cgagttcgcc accgccgatc acgcaaatac
     1741 ccttgccagc ttcctgctta agctgccgca caaattcagc agagttccgc gaaatcactt
     1801 ctagtttctt gctgcgggtt tgtttgagcg tgcgcgagaa gacgtagttc ttgacgcccg
     1861 gataggctcc gctgcccatg cgagccgcca cttcataagt cttgcgaccc attacgacgg
     1921 tgtcgatggt cttccagaat tcccgggaga tcgccgcaac ctcgtcgtcc caaacaagcc
     1981 aatcaaccgc cccatccgcg cgggcaatga aattgtcgag actgttggca acgccaaacg
     2041 taactgtgcg catgatcaaa gtactcgaag ttcgcggact caaatggcgt tcttcgtgtc
     2101 aaaaataaat atcttcaatc ttatcgacaa gttcagcgag gagcgcgttg ttgcgatttg
     2161 cctggacgat gatcgtgaac ttgcgatcca ggtagcgccg gatgaattga gtgcaaccga
     2221 tcgtgttgcc gctgtgccaa acagttcgta ggccgcgtcg cttctcgata aaccagccaa
     2281 agccgtaacc ggtgtcttca tcgaccctgg cgccactggt gaaggcttgt tgcaaggttg
     2341 ctctcttcac caggcgactg cttcggagcg cccggtccca cttgtaaaga tctgcaactg
     2401 atgaatagac cgtgccatcg ccccgagttg aactggtcaa actctgatca gttcgttcga
     2461 agacttcgcc acgcttcgag taaccatagg cacgatgagc ttcgtctgaa cgatcgtcgc
     2521 gctggtaaag cacggtgccg tccatcttca acggagcaaa aatgttccgc cgcaggaatt
     2581 ccggaaacga catgcccgcg gctttctcga caatcaaacc cagcagcaca tagccgctgt
     2641 tgctgtagcg atatgatgaa ccgggcggga aatgtgtgtg gtcctggcgc ttcatcagtt
     2701 cgagcacgtc ttgatcggta agtggcacag tcgcgttttc gtccatcaca tcctcgtacg
     2761 cgattaagcc cgagctgtga ttcagcagat gacgcaccgt gacttgcttc ccgtattccg
     2821 gaaagccggg aaaaaagctg gccaaactat cgtcatacga aagctgcttg cgttcagcga
     2881 gaatcatgat cgccatggca gtgaactgtt tggtgacgga ggcaagccga tagtttgtgt
     2941 tgggtgtcgc cgcaatcttc gcttcaaggt tcgccagacc atatgctttc ttaaagagca
     3001 ccttgtcgtt tgcaatcacg atgacgctgg cgccgggggc gccgggttgc gcgtagtcac
     3061 tgaagagtgc gtccatggcc gccgcctttt cagccggagt tttcatagga gatgagaaga
     3121 gaactggagt gatgaccagc gccaacacga cttggtaagt tagtttcatt ggatgctcgc
     3181 gaagcggaaa gccattttag aacttcttcc cagcaatcag ccaccaggca cttacggcgc
     3241 tcaagagggc gagcagcgcc gaaccgaatg ctacgcgacg aaacccagtc acaaacgatt
     3301 cttcgatcgc ttggtgcaac ttagtcttcg ttgcttcatc gagctccgca ggcggctcag
     3361 catccgccag gcgcacgcgc tgctgatcca actcgtgcct aacctgcggc gccagctgca
     3421 gcgcgttcaa acgttcatcc aggcgactgt tgaatccttg cagcatcacc aggccaaata
     3481 ctgcgaccgc cagcaaaccg gctgttcgcg aaacggcgtt gttgattccc gaagcaacac
     3541 cggcgcgatt ctccttcacc gagctcatca cagttgttgt taagggcgca acgctgatgg
     3601 ccatgccgag tcccagaact acgacggcgg gaaagaacgt ttgccaatag ctgccgccga
     3661 caccgggcag catgaacaat acgaaaccca ctgccacaat gattggccca atgatcagcg
     3721 gcagtttcgc gccatagcgc ttaatcaacc caccagacca gcgcgatagt aagaacatca
     3781 gcagaataaa cggcaacaac gcggccccgg ctgctgtcgg cgagtagccc tgcacctgaa
     3841 tcagattaag cggtagaaaa aagagcgccc cgctgagggc ggtatagaga aagagcgtca
     3901 gcaggtttgc gccgctaaag ttgcgcgagc gaaacattgt cagtggtaac atcgggttcc
     3961 gcttccgcgc ctcaacaaat agaaacaaga ccagtgtcac aactccaaca caaagggccg
     4021 cgaggatcag cgggtgcgag aagccgagtc ttgaggattc aatcaagcca aagacaatcg
     4081 cgcccagact aatagttgcg aaggtggcgc cccaccaatc aagaccctgc gattcttcgt
     4141 cgcgactctc aggcacgtgc agaaaaacaa gcaccagcac gatgatggcg aacggtgcgt
     4201 tgatgaaaaa gacggcccgc caggaaacct tttctattaa ccagccgccc agtaccggcc
     4261 cgacggcagc cgtgatcgcc gtaaaacccg accaggtacc gatcgcgcga ccgcgttcgt
     4321 cggccgcgaa agcattgctg attattgcca ggctcccggg taccagcaga gcaccgccaa
     4381 ttccctgggc ggcacgcgcg atgatcagtt gattaatgtt gggagcgagt ccgcaccaaa
     4441 ccgaagcgag ggcaaaaagg aaaacaccga tgatgtaaac gcgtctgcgt ccgaagtgat
     4501 cgcccagcga accgccggtc aggaggaggg cggcaaggaa cagagcgtaa gcttcgacca
     4561 cccattggac gtcgacaacg gtcgcgttga gattagactg taaggcagga agggcgacgt
     4621 tgacgaccgt gccgtcaata aagaccatgc tggagccaag aatagtagcg gctagaatcc
     4681 aggctccact tgtctttttg cgcatcgctg cattttcatg cagcagggaa gtaagggcaa
     4741 actcagtgcg gtctttagtt ccaggttatt cgttcggatt ccaagcggga tcgtaaacga
     4801 accacggcgc gtggcgattc ttccaataaa agagaatagt gttgaagctg ggaattacca
     4861 ccaggagttg gaatacaaag atgagcaagc c
//
LOCUS       DASUEX010000174         3987 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1402796, whole genome shotgun sequence.
ACCESSION   DASUEX010000174 DASUEX010000000
VERSION     DASUEX010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3987
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1402796"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            498..1217
                     /locus_tag="VFV61_02870"
     CDS             498..1217
                     /locus_tag="VFV61_02870"
                     /inference="COORDINATES: protein motif:HMM:NF016269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HEX4899444.1"
                     /translation="MRATKIMAAVMIALVISFGPWVKKSTARTNSDDDTTKRLNAAAD
                     VLKEIMSAPDKGIPQDLLDKAACAVIVPNVKKGAFIVGAKYGRGFIVCRNSNGRGWSA
                     PGGVKVEGGSVGFQIGGSETDIVMLLMNQGAIDKLLSSKFTIGGDASVAAGPVGRTSS
                     AATDAQLHAELLSYSRARGLFAGVSLDGATLRPDDDANKDMYGKEMSNKDVVLGNMKP
                     PRTAARLSAELNKHSSRRAGS"
     gene            1331..2773
                     /locus_tag="VFV61_02875"
     CDS             1331..2773
                     /locus_tag="VFV61_02875"
                     /inference="COORDINATES: protein motif:HMM:NF024833.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HEX4899445.1"
                     /translation="MKIKHTEDRFVAIILGFTLLFGVGLASSAIGHVRDNNGDYNQNP
                     QDRNWNNYPNWGGSFELRQTALNAGYNEGAKEGRNDYARGRRSNLQDFSAYQEATKDY
                     NSRLGDRELYRRYFRRAFENGYNSELQTTYDSTSRLNGTFRLDSSRSDNPRNVANRAT
                     RNLRGNERQGVSARMLARLESPEMLAIERRYSTITMASSLAPQSTFEADGVERQEQLD
                     GRTIQVTATLRGDQLVVRSTGYRNNDFTATFEPIESGRRLRVRREVYSDRLTQPVVVN
                     SIYNRSSDVAQWNVYDGSQPGGTTVTSGEFIVRDGETVVAVLNSDLTSQQAKQGDRFT
                     LTVREPGQYEGAVIEGTVGSVDRGGRLTGRSGMSLNFDTIRLRNGRTYRFAGVLGSVR
                     TPNGDTVKVDNEGTAQGENQTTQTVQRTAIGTAVGAIIGAIAGGGKGAAIGAIIGAAG
                     GAGSVYVQGKDNLELPTGTEITIRSSAPSQ"
     gene            complement(2918..3613)
                     /locus_tag="VFV61_02880"
     CDS             complement(2918..3613)
                     /locus_tag="VFV61_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HEX4899446.1"
                     /translation="MPLFHPERHRTQHIGWLRAAVLGANDGIVSTASLVVGVAASHAT
                     HTAVLVAGVAGLAAGAMSMAAGEYVSVSSQLDTERADIERERTELAGNEDYERKELAA
                     IYVKRGLELSLANQVADQLMAKDALGAHARDELGISETISARPVQAAITSAITFAVGA
                     ALPLLVVVIAPATRLGLMVAGTSLFFLALLGALAAYTGGAPILKGIFRVTLWGTLAMA
                     ITAGIGALFGTTV"
     gene            complement(3660..3902)
                     /locus_tag="VFV61_02885"
     CDS             complement(3660..3902)
                     /locus_tag="VFV61_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899447.1"
                     /translation="MNETRKTPLVIALVVVVVLFLIFGGWAMAGMGGGMMGQGGIGGY
                     RIGHGWINGGFSWLWIPTLLTLGLGVLLGWLIFGKK"
BASE COUNT          983 a         1076 c         1084 g          844 t
ORIGIN      
        1 cggaacttat tgttcgcgag cgccgattgc tggacatttt ggaattgcat aatacgcgca
       61 ctcctcccgt ttatgattct gtttaactct cggataggca aaagacatgc cctcaaaaat
      121 ctctcgacct tcggctcggg ggaaatcaaa aacgctcttc gcgaaagtgg aatgcttcca
      181 aattaacctc cagtatcaga agcgcggtat atcccaaacc gtgacgtagg atttcttcgc
      241 gtaattctcc tgatttactg cgcgaatttt cgcaacgatc ttcaaccaac aactcttctt
      301 cgagatggca ggacaaaaca aggccagttt gataacacat taaagctgct atgtgcgctg
      361 gcacacataa gtcgccaagg ctcggaactt gcagtgttat atcacataca aggttaggcg
      421 taattgtgtc caacggaacg gttggtttgt ttcgcggaaa atgacaagac aagcgagatt
      481 caaatggggg aggaaaaatg agagcaacaa aaataatggc agcggtgatg attgccttag
      541 tgatcagttt tgggccatgg gtgaagaagt caaccgctcg tacgaacagc gacgatgaca
      601 ctacgaagcg gcttaacgcg gcagccgacg tactgaagga aatcatgtcg gcgcccgaca
      661 aaggcatccc gcaagacctt ctggataagg cagcgtgcgc cgtgattgtg ccgaacgtaa
      721 agaaaggcgc tttcatcgta ggcgcgaaat atggacgagg ttttatcgtg tgcagaaaca
      781 gcaacgggcg cggttggtcg gctcccggcg gcgtgaaagt ggaaggcggc agtgttgggt
      841 ttcaaattgg cggatccgaa actgacatcg taatgctgct tatgaaccag ggcgccatag
      901 ataagctcct gtccagcaaa ttcactatcg gaggcgatgc ttcagtagcg gcggggccag
      961 taggccgtac gtcttcggct gcgacggacg ctcagcttca cgccgagctt ctcagctatt
     1021 ctcgagcgcg cggattattc gcaggcgtct cactggacgg cgccactctg cggcccgacg
     1081 atgacgcaaa taaggacatg tacggcaagg agatgtcaaa caaagatgtg gttctgggaa
     1141 acatgaagcc accgcggacc gcagccaggc tgagcgcgga gttgaataag cactccagcc
     1201 gcagagctgg tagttagcga accctgaaca ggagggtgcg acttgaataa ggctcattcg
     1261 tctttcgcct gtcttgtcgc gtcaatcgct tgggtgtcaa ctagatatct aagcaagaaa
     1321 ggaatctaat atgaagataa aacacacaga ggacagattc gtggcaatca ttctggggtt
     1381 cacgttactc tttggcgttg ggctcgcttc cagcgcaata ggacatgtgc gcgataacaa
     1441 tggtgactat aaccagaatc cccaggatcg taactggaat aattacccga actggggtgg
     1501 ctctttcgag ctgcgccaaa ctgctctgaa cgcgggctac aacgaaggcg ctaaggaagg
     1561 ccgcaatgac tatgcgcgag gaagacgttc taacctccag gactttagtg cttaccagga
     1621 agccacgaaa gactacaact cacgtctcgg cgaccgcgaa ctatatcgcc gctacttccg
     1681 tcgtgcgttc gagaatggat acaactctga actccaaact acatacgact caactagtcg
     1741 cttaaacggg accttcaggt tggattcgtc gcgcagcgac aatccccgca atgttgccaa
     1801 tcgtgcaact cggaaccttc gcggcaacga gcgacagggt gtctcagcgc gcatgctggc
     1861 gcgactcgaa tcacctgaga tgttggcaat cgagcgccgc tactcaacca taacgatggc
     1921 ctcctcgctc gctccgcagt cgacctttga agcggacgga gtcgaacgtc aggagcaact
     1981 cgatgggcga acgattcagg taactgcgac gttgcgcgga gatcagttgg ttgtgagatc
     2041 gaccggttac cgaaacaatg atttcaccgc cactttcgag ccaatcgaaa gcggccgtcg
     2101 gttgcgcgtg aggcgcgagg tctactcgga ccggctgacg caacccgttg tcgtcaatag
     2161 catctacaac cgttcctcag atgtcgcgca gtggaatgtt tatgacggct cgcagccggg
     2221 cggcacaacc gtaaccagcg gtgaattcat cgttcgcgat ggcgaaactg tagtggcggt
     2281 cctaaatagt gacctgactt cacagcaggc aaagcagggc gaccggttca cgctgaccgt
     2341 gcgcgagccc ggccaatacg aaggcgcagt tatcgaaggc acggtgggta gcgttgaccg
     2401 tggtggacga ctgactggcc ggtccgggat gtcgctcaac ttcgacacga tccggctgcg
     2461 taacggtcga acttacaggt tcgccggagt tctcgggtct gtacgaacgc cgaatggaga
     2521 taccgtcaag gtagacaacg aaggcaccgc acaaggcgag aaccagacca cgcaaaccgt
     2581 ccagcgcact gctattggca cagcagttgg cgcgatcatt ggcgcgattg ccggtggcgg
     2641 gaagggagcg gcgatcggcg caatcattgg tgcggctggg ggagccggct cagtttatgt
     2701 tcaggggaaa gataacctcg agcttcccac cggtacggaa ataacgattc ggtcgagtgc
     2761 accaagccaa tagctggtca gggagcaact gtcgctatcg cggcggcggt ctcctaattc
     2821 tgtaggggcg gcactccgga gttgacgccc ctacaggctc gtcaatatag gacatcacct
     2881 tcgtgtgaga acgatctgat cgacttcgga ttcgacgcta cacagtggta ccaaacaacg
     2941 ctcctatgcc agccgtgatt gccattgcaa gagtccccca caacgtgaca cggaatattc
     3001 cttttaggat gggggcgccg ccggtataag cggccagcgc gcctagcagc gcaagaaaga
     3061 ataaggacgt tcctgcaacc attagcccga ggcgggtggc aggggcgatg acgacgacga
     3121 gtaaagggag tgcggcccca actgcaaacg ttatagccga agtaatcgcc gcttgaactg
     3181 ggcgcgcact aatggtttct gagatgccga gttcatcgcg ggcatgtgct cctagtgcat
     3241 ccttagccat tagttgatcg gcgacttgat ttgccagact caattcgagc ccacgtttga
     3301 cgtagatggc tgcaagttcc tttctctcgt agtcctcatt gccggcgagt tctgttcgtt
     3361 cacgttcgat gtctgctcgt tcagtatcca attgagagct aaccgaaaca tattcacccg
     3421 cagccatgga catggcgcct gcagctaagc ctgcgacacc tgccaccaaa accgcagtat
     3481 gagttgcatg tgatgcggcc acaccaacaa ccaggctggc agttgagact atgccatcgt
     3541 tcgcgcccag aacagccgcg cggagccaac ctatatgctg tgtacggtgc cgctcaggat
     3601 ggaataaggg catcttattt tccctaccag acaatctggc aattcatcat ttggatcggt
     3661 cacttcttac cgaagatcag ccagccaagc aggacgccaa gaccgagggt aagtaaagtg
     3721 ggaatccaca accaactgaa cccgccgttt atccatccgt gtccaatcct gtacccgcct
     3781 atccctcctt ggcccatcat cccgccaccc atccctgcca ttgcccagcc gccgaagatc
     3841 agaaaaagca ccacgacgac gaccaacgcg ataaccagag gtgtcttcct tgtttcattc
     3901 ataacctaag ctcctcggtt tacatcttct tcatctccat ctttttgttg gccgctttgc
     3961 ttccaccatt catcttcgac atcatgc
//
LOCUS       DASUEX010000175         3819 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1163316, whole genome shotgun sequence.
ACCESSION   DASUEX010000175 DASUEX010000000
VERSION     DASUEX010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3819
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1163316"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3399
                     /locus_tag="VFV61_02890"
     CDS             <1..3399
                     /locus_tag="VFV61_02890"
                     /inference="COORDINATES: protein motif:HMM:NF020083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899448.1"
                     /translation="QRVGRSNHTVAGFPKGRIFPLSRDELVECAALIDAVRRGELDRL
                     SIPEKPLDILAQQVVATVAPEEWTEDGLFGMVRRAYPYRNLERKEFDSILRMLAEGFS
                     TKRGRRSTYIHHDAVNQRLRGRRGARLAAITSGGAIPDTADYAVVLEPNGLVIGSVNE
                     DFAIESLQGDIFQLGNTSWRVLRVEQGKVRVEDAHGQPPSIPFWLGEAPARTHELSTA
                     VSRLREEVAARVQGPTANVQSQDEWNEDEATQVKGWVLQDAIDWLMNQVGISQSAAEQ
                     IVEYLASAKAALGVMPSLDNIVLERFFDDSGSMQLVVHSPFGSRLNRAWGLALRKRFC
                     RKFNFELQAAATEDAIILSLGPTHSFPLEDVFHYLNSKTVRELLCQALLDAPMWNIRW
                     RWNVTRALAVLRRRGGKKIPAQLQRMDAEDLLTAVFPDQVACAENLTGQREIPEHPLV
                     AQTVKDCLEEAMDVEALEALLSAIERNEKNLFARDLLEASPLAAEILNARPYAYLDDA
                     PLEERRTRAVYQRRWLDPETASDLGKLDQAAIDRVREEVWPEPRNADELHDALVELGV
                     LTAAEGAQWQEYFIELQNDRRAAVLRTVPGPPLWVAAERLPQLSVIYPQASLMPAIKA
                     PESLADANWSFESALVEVVRGRLEGLGPTTVPELASSLALLTNDVEGALLRLEAEGFV
                     IRGKFTPGLDQTEWCARRLLARIHSYTLNRLRQEIEPVNASDFVRFLLAWQKVAPEHQ
                     MEGPESVRAIIEQLEGFEAPAAAWEGEIFPARIVEYDPAWLDALCLSGEVMWARLTPP
                     SQKNGDQRPRGSGPVRTTPIALIRRQNFAVFNSLFPVRIEELKFSTVTEAVFDYLKNR
                     GASFFTDIVEGTKLLRSQVEEALAEMVAQGMVISDSFTGLRALLTPAHRKTQAGARRK
                     HREPVYDMASAGRWSLIQRNPAVREGSTEAQENAEKIARLLLKRYGVVFKRLLEREGL
                     TIPWRVLLRLYHRLEARGEIRGGRFVAGISGEQFALPEAIGMLRAIRRARVGNAEFVV
                     RTPEDQHTIGGDAGISSNSESLISISAADPLNIVGIITPGARITAHTSNRVLYHNGEP
                     IAVLEAGETKYLSQLSRAMEWKAKAALLRKATPPQLRSYLRRPA"
     gene            3583..>3819
                     /locus_tag="VFV61_02895"
     CDS             3583..>3819
                     /locus_tag="VFV61_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899449.1"
                     /translation="MRTTFQFIVSPLVLLVALSVSLSAQEKEKKEPQFKLVEFHMALL
                     KLGPNGNATNSEQAKAVHDEQQAYFQELVKSGKAV"
BASE COUNT          836 a          958 c         1159 g          866 t
ORIGIN      
        1 cagcgagttg gtcgttcaaa ccacacggtt gctggtttcc cgaaggggcg gatctttcca
       61 ctctcgcgcg acgagctggt tgaatgtgcg gcgttgattg atgccgtgcg gcggggcgaa
      121 ctggatcgac tatcaattcc agaaaagccg ctcgacatcc tggcccagca ggtggttgcc
      181 accgtggcac cggaagaatg gactgaggat ggacttttcg gcatggtgcg gcgcgcttat
      241 ccgtatcgaa acctcgaacg aaaagagttt gattcgattt tgcggatgct ggctgaaggt
      301 ttttccacga agcgaggtcg ccgctctacc tacattcatc acgatgcggt aaatcagcgg
      361 ttgcggggtc gccgcggtgc gcgactcgca gcaattacgt caggcggtgc gattccagac
      421 accgcagact acgccgtagt gctcgaacct aatggcctgg tgatcggcag tgtcaacgaa
      481 gactttgcga tcgagagcct gcagggtgac atctttcaac tcggaaatac ctcttggcga
      541 gttctgcgtg tcgagcaggg aaaggtgcgc gtcgaggatg ctcacgggca accgccctcg
      601 attccattct ggctaggtga ggctccggcc aggacccacg agctctcaac cgctgtttcc
      661 cgcttgcggg aggaggtggc ggcgagagtt caaggcccaa cagccaatgt tcaaagtcag
      721 gatgagtgga atgaggatga agctactcaa gttaagggct gggtattgca ggacgcgatc
      781 gactggttaa tgaatcaagt tggcatctcg cagtcggccg ccgagcagat tgttgaatac
      841 cttgcctcag ccaaagcggc gcttggagta atgccttcgc tcgacaatat tgtgcttgaa
      901 cgcttctttg acgatagcgg cagcatgcag ttggtagttc attcaccctt tggcagcagg
      961 cttaaccgcg cctggggact cgctctgcga aaacgttttt gccgtaagtt caattttgag
     1021 ttgcaggcag cggccactga agatgccatc attctgtcgc ttggtccaac acacagcttt
     1081 cctctcgaag atgtgttcca ttatctcaat tcaaaaactg tacgcgagtt actttgccag
     1141 gccctgcttg acgcgcccat gtggaacatt cgttggcgct ggaatgtgac gcgtgcgctg
     1201 gccgtgcttc ggcgccgtgg tggaaagaag atccctgcac agcttcagcg aatggatgcg
     1261 gaagacttgt tgaccgcagt ttttccggat caagtggcat gtgcggaaaa tctgaccggc
     1321 caacgcgaga ttcctgaaca tccgttggtg gcccaaacgg tgaaagactg tttggaagag
     1381 gccatggacg tcgaagccct cgaagctctg ctgagcgcca tcgaacgaaa cgagaagaat
     1441 ctcttcgcac gcgatctgct ggaggcttca cccctggcgg ccgagattct caatgctcgt
     1501 ccatatgcct acctggatga tgctccgctg gaggagcgac gcacacgtgc ggtctatcag
     1561 cgtcgctggc ttgatccaga gacagcgtcg gatctgggca agctggacca ggcggccatt
     1621 gaccgggtac gggaagaggt ttggccagag ccgcgaaatg ctgacgagct tcatgatgca
     1681 ctggtggagt tgggcgtttt gactgctgca gagggagcgc agtggcaaga atattttatt
     1741 gagttgcaga atgatcgacg cgcggctgtt ttgagaacgg tcccaggtcc gccgttgtgg
     1801 gtggctgctg aacgactgcc acagttgagc gtcatctatc cacaagcgag cttgatgccc
     1861 gcaatcaaag cgcctgagag cctggctgac gcaaactggt cgtttgaaag cgcgctggtg
     1921 gaagttgttc gcgggcggct tgaaggactg ggaccgacga ctgtgcctga gcttgcaagt
     1981 tcgcttgctc tcctgaccaa cgacgttgag ggcgcattgc tgcgccttga ggcagaaggt
     2041 tttgtcattc ggggcaaatt tacgcccggc ttggatcaaa ctgaatggtg tgctcgccgc
     2101 ctgctcgcac ggattcacag ttacacgctc aaccgactgc ggcaggagat cgagcctgtc
     2161 aacgcctcgg attttgttcg tttccttttg gcctggcaga aagttgcgcc cgagcatcag
     2221 atggagggac ccgaaagtgt tcgcgcgatc attgagcaat tggagggctt cgaagcgccc
     2281 gcggccgcat gggaaggcga gatctttcct gcgcggatcg tggaatacga tccggcctgg
     2341 ttggatgctt tatgcctgtc gggagaggtg atgtgggcga gactgacgcc cccgtctcag
     2401 aaaaatggcg accaacgacc acgtggatcg ggtccggtcc gaaccacacc aattgccctg
     2461 attcgacggc agaactttgc ggtcttcaat tcgctgtttc ccgttcgcat cgaggagttg
     2521 aagttctcaa cggtaaccga ggccgttttt gactacctca agaatcgcgg cgcgtcgttt
     2581 ttcaccgaca tcgttgaggg cacgaaacta ttgcgtagtc aggttgaaga agcattggcg
     2641 gaaatggtgg cgcagggcat ggttatttcc gacagcttta ccggcttgcg cgcactgtta
     2701 acgccggcgc accgcaagac tcaggcgggc gcaaggcgca agcatcggga acccgtttac
     2761 gacatggcaa gtgcaggtcg gtggtcgctc attcaacgaa acccggcggt tcgcgagggt
     2821 tcgactgagg ctcaggagaa tgccgaaaag atcgcgcgcc tgttgctcaa gcgctatggc
     2881 gtcgttttca aacgtctact tgagcgagaa ggattaacaa ttccgtggcg tgtgttgttg
     2941 aggctttatc atcgtctgga agctcgaggc gagattcgtg gtggtcgttt cgtggctgga
     3001 atttccggcg aacagtttgc cttgccggaa gcaattggaa tgttgcgagc gatcaggcgt
     3061 gctcgagtgg gtaatgcaga gtttgtggtg cgtacgccgg aagatcagca caccattggt
     3121 ggtgatgcag ggatctcttc aaattcagag agcctgattt cgatcagcgc ggcagatcca
     3181 ctgaacatcg tcggtatcat cacgccgggt gcacgtatca cggcgcacac ttccaatcga
     3241 gttctctatc acaacggcga accgatcgct gttctggaag cgggcgagac aaagtatttg
     3301 tcacagttaa gccgggcaat ggagtggaaa gctaaggcgg cgctcctgag aaaagcaacc
     3361 ccgccccagt tgcgatcgta tctaagacgt ccagcttgaa ccccggggct cgtaatatga
     3421 gttcgcgaat cgaatggggt tattgttcat agcctgcgaa gcaggcgtga gcataaagcc
     3481 tgggggcgta agccccaggg ggccgttctt taaaggaatt tgaggcgaag gtttaactgg
     3541 cacttgcaag tctcaatcta aaccctagca aggaatcgca tcatgagaac cacatttcag
     3601 tttattgttt cccctttggt gcttttagta gcgctttcag tctcacttag tgcccaagaa
     3661 aaggagaaga aggaaccaca gtttaagttg gtcgagtttc acatggcatt attgaaactc
     3721 gggccgaatg gcaatgcgac caattcggaa caagcaaagg ccgttcatga tgagcagcag
     3781 gcatactttc aggagctggt gaagtcaggc aaggctgtc
//
LOCUS       DASUEX010000176         6751 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_837132, whole genome shotgun sequence.
ACCESSION   DASUEX010000176 DASUEX010000000
VERSION     DASUEX010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6751
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_837132"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1197
                     /locus_tag="VFV61_02900"
     CDS             <1..1197
                     /locus_tag="VFV61_02900"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX4899450.1"
                     /translation="SVCVLFLFYITQAIVTRRTAIVLVCIMLASGIAGTFYSLFDLTR
                     GRGVIVATIKNDSPLRQAHIEAGDAVWRVNGKRVYSTADVDAALKSAPADKTVAVSVI
                     SRGEHVERPGLVLTEKLKQQATPAGITGTERTHRFRASGWTRHYETYSEILQVLAQLG
                     LGLALAHLRNHGARLRFKLAIAATAILALGIALTAMRTVLVAFVIGAGIILLRATKGK
                     TKLLATAALGLVLAFGAFVVWQTRAQDALTLRDSSASLRVAVARVGARRIFLHPVFGH
                     GMDAVHKHWNEWGFPGDQLLGLHSTPLQLAFDRGLPALLCWLWIMLTFWLLTVRAEHR
                     IRDTGDTNSYGLLLGATGALAGFFASSLVNYNFGDAEVALVFWWLMGIVVVLSYKERL
                     AAPGNV"
     gene            1217..1864
                     /locus_tag="VFV61_02905"
     CDS             1217..1864
                     /locus_tag="VFV61_02905"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX4899451.1"
                     /translation="MLTLLIVALMIGVSPSFAGTDILGKEVTARRRDNEVARATWRPS
                     SPQTTRRKTRKRKPDVIYYPTPPETVAEMLRLAEIKKGDVLYDLGSGDGRIPIAAAKE
                     FGIRAVGIEIDPKLISEAESNARSAGVAQLVRFRNEDMFRINVSEANVVTLYLSEKLN
                     VLLRPKLLRELRPGSRIVSHDFRMGNWKPEQTVRVPWGKLYRTVYVWTVPKRSKL"
     gene            complement(1951..2775)
                     /locus_tag="VFV61_02910"
     CDS             complement(1951..2775)
                     /locus_tag="VFV61_02910"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX4899452.1"
                     /translation="MISAQTARRAVWRPLWELPSRFELILSLARRELLARYKGSALGV
                     VWALLTPVVMIAIFTFIFAGIFGARFGASASPWDYAIHLFCGLLPWTMFQEAVLQSST
                     TIVSHANLVKRVVFPLETLPIAQTLSALGNQLFGTVALLIAILLIRQQFPVSIIWLPV
                     LLIPQFLLTLGIAWLIASLGVFLRDIAQGINLFMMAWMFLTPIIYPESIVPARYRPFV
                     NLNPFTPLIRSYRRVLIEGTAPDWAGLAYFTAFALVSFIFGYWWFARTRKNFADVI"
     gene            complement(3049..3177)
                     /locus_tag="VFV61_02915"
     CDS             complement(3049..3177)
                     /locus_tag="VFV61_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899453.1"
                     /translation="MSHVRFGFAAGSEAVAMPRRSGALYMLGYAQWCGLWPNKVKD"
     gene            complement(3269..5824)
                     /locus_tag="VFV61_02920"
     CDS             complement(3269..5824)
                     /locus_tag="VFV61_02920"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899454.1"
                     /translation="MKLVLRMLCALIVLTLASGVFFSALAQTPSATPEPFVSQLTSGA
                     AGFNSFAKDVTANGRFVVVESNGDIATEKIPNRDSAGNLNPNPRNNEDGNREIFLIDY
                     AQRRIFQITNTKSVPKATPTPTPTPTPTPTPTPNPSPTPTPTPTPTPVPTPPDPANIQ
                     IEISNNRPMITLVPGTNGHYRIVFSANAPHPGLFDGVDTGGVLAADGNQEVWIYELPI
                     ITEVDLTTGADLPFQDLTLGTFTKITDTPASRVPSPGATGIFPFVADDNRDATISETG
                     EIIAFISTRNLASNNADGNPELFLHTISAPGVFVQGTATADSFVGPVLFSVFQNSPSL
                     SADGTVVAFISNGNLSGNNNNDDGNGHGNAEVYIGTFNGATFTVTRQATRTKADASGP
                     AFGATVNVLSPGRRLSRDGTLVAFESLADDPKANATTNQPFLAVFVYTIAQDKFDLVG
                     KRAIGFPGDVIHFPTFTDYNSSLAPATLIYASALNFKVDGTFPPAAEDSTGLNPSRAV
                     QVFSTSLPITTTNTFRRLTKNSVFAAFSPLTTATIERTVFSAGGAELGGGNPDGSTEV
                     FYLLSPPVTTESAGTLSFFTGASNVALPEASPSPSPSPTPTPSPSPGTATGLAPGELG
                     IIRTSVEFVAADATATGGSETKRSPALPVELNGVSVAVNGAAAGLYFVGQASKQINFV
                     VPIGMTAGVKTVVVHSRMAGGIQFRGSLLIVPAQPDIFTTTNDAGGRAAVFNVTNPMA
                     RTPEPFSVTSTDGSGNTVATVLEVNLTGVRTATAAEVKVSIVTATATTDITGDSIVLV
                     SPNREMPGWDLIQFRLPASLAGAGDVPIIVTITRGGATFVSRPAATAPHITIN"
     gene            6104..>6751
                     /locus_tag="VFV61_02925"
     CDS             6104..>6751
                     /locus_tag="VFV61_02925"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4899455.1"
                     /translation="MTTALRVEGVSKRYRIYERPNDRLREMLTRGRWKRHQEFWALKD
                     VSFEIEAGTTTGIIGPNGSGKSTLLQIMTGTLEPTHGSVWHEGRIAALLELGSGFNTE
                     FTGIENVFMNASLMGFSRSETEALLPEIERFAEIGPFIYQPVKTYSSGMYVRLAFSVA
                     ISAAPQILIIDEALAVGDAVFQHRCMRRLKEMQESGVTIFFVSHDLASVRALCSRA"
BASE COUNT         1554 a         1789 c         1915 g         1493 t
ORIGIN      
        1 tccgtctgcg ttcttttcct gttctacatt acccaggcaa tcgttacccg ccgcaccgca
       61 atcgtcctcg tgtgcatcat gctggcttcg ggtatcgcgg ggacctttta cagcctgttt
      121 gatctgacgc gcgggcgcgg ggtgatcgtg gcaacaatca agaacgacag tccgttgcgg
      181 caagcacaca tagaagctgg cgacgcagtc tggagagtta acggcaagcg tgtctactcg
      241 actgcagatg ttgatgccgc actgaagtcg gcgcccgcag acaagacagt tgctgtcagt
      301 gttatttcac gcggtgaaca cgtagaacgt cctggcttgg ttctgacgga aaagctgaaa
      361 cagcaagcaa cgcccgcggg cattactggt accgaaagga ctcatcgatt tcgcgcttca
      421 ggttggacgc gtcactacga aacgtattcc gagattctgc aggtcctggc ccagcttgga
      481 ctcggtctgg cgctggccca tctgcgaaat cacggcgcgc ggttacgctt caaattagcg
      541 atcgccgcca ccgcgatcct cgcgcttggc attgctttga ctgcaatgcg taccgtgctg
      601 gtagcgtttg tgattggtgc gggaataatc cttttgcgtg ccacaaaggg aaaaacgaaa
      661 ctgctggcta cggccgcgct gggtctcgtc ctggccttcg gtgctttcgt tgtttggcaa
      721 acccgcgccc aggacgcact gaccttgcgg gattcgagcg cgtctttgcg ggttgccgtc
      781 gcgcgcgtgg gtgcaaggcg aatttttttg catccagtgt ttggtcacgg gatggatgcg
      841 gtacacaagc attggaacga gtggggcttt cccggtgacc aactgctggg acttcattcc
      901 acgcccctac aactcgcttt tgatcgcggc ctcccggcct tgctctgttg gctgtggata
      961 atgctcacct tctggttgct gaccgtccgg gcagagcacc gtatccgcga tacgggagat
     1021 accaacagct acggtttgtt gcttggtgcg accggcgcgc ttgccggctt cttcgctagt
     1081 tctctggtta actataattt tggcgacgct gaggtggcgc tcgtgttctg gtggttgatg
     1141 ggaatcgtag tagtgctcag ctacaaggaa agacttgctg ctcctggaaa cgtttaagcc
     1201 acgacctttt ttacatatgc tgactcttct aatcgttgcg ttaatgattg gcgtcagccc
     1261 gtcgtttgcc ggaactgaca tcttgggcaa ggaagtcaca gctcgtcgta gagacaatga
     1321 ggtcgctcga gccacttggc gcccgagcag cccacaaacc acgcgaagaa aaacgcgcaa
     1381 acgtaagcct gatgtgatct attacccaac gcctcccgag acagtggccg agatgctacg
     1441 cctggcagaa attaagaaag gtgatgtcct ctacgatctg ggcagcggtg acgggcgcat
     1501 tccgatcgcc gcggctaaag agtttggcat tcgggcggtg ggtatcgaga tcgatcccaa
     1561 gctgataagt gaggccgagt cgaatgcgcg cagcgccggc gttgcacagc tggtgcgctt
     1621 tcgcaatgaa gacatgtttc gcatcaatgt tagcgaagca aatgtggtta cgctctatct
     1681 gtctgagaag ctcaatgtac tattgcgccc gaagttgttg cgcgaactgc gaccgggatc
     1741 gcgtatcgtt tctcacgact tccgcatggg caactggaaa ccggaacaaa cagtccgtgt
     1801 tccgtgggga aagttgtatc gcacggttta cgtctggact gtgccaaagc gctcaaaact
     1861 ctgactcaat gcggcagagc cggtgtcgct gctctggcgg caaagccgaa cggtccacta
     1921 tctgagtttt gagacaaagc tgacgagccc tcagatgacg tcggcaaaat tcttgcgcgt
     1981 acgcgcaaac caccagtatc cgaagatgaa gcttacgagg gcgaaggcag tgaagtatgc
     2041 gaggccggcc caatccggag cagtcccttc aattaagacg cggcgatagc tccggatcag
     2101 cggagtgaag gggttgaggt ttacgaacgg ccggtagcgc gcgggcacga tcgattctgg
     2161 gtagataatc ggcgtcagga acatccacgc catcatgaag agattgatcc cctgcgcgat
     2221 gtcgcgcaga aaaacaccca gcgaggcgat cagccaggcg ataccaagcg tcaaaaggaa
     2281 ttgggggatc aagagcaccg gtaaccagat aatcgagacc ggaaattgct gacgaatcag
     2341 aagaatcgcg atcagcaagg cgacggtgcc gaaaagttga tttcccagtg ccgatagcgt
     2401 ctgagcgatc ggcagtgttt cgagaggaaa gacaacgcgc ttgacgagat tcgcatgcga
     2461 aacgatcgtc gttgacgatt gtagtactgc ttcctgaaac atcgtccacg gcaacaatcc
     2521 acagaagaga tggatcgcat aatcccaggg tgaagcgctg gcaccaaagc gggcgccaaa
     2581 aattcccgcg aagataaacg taaagatcgc aatcattact accggcgtga gcaacgccca
     2641 caccacgcca agcgccgagc ctttgtagcg ggccagcaac tcacgccgcg ccagcgaaag
     2701 gattaactca aaacggctcg gcaattccca gagcgggcgc cagacggccc ttcgtgctgt
     2761 ttgcgcagat atcatgtgtt cgtgatagta aatcctgaat aagttttata gaagttcagt
     2821 tccgcaggga agttttagaa tcagcccttg atgggcacgg ctgatcactg attcaaaaaa
     2881 acctcttgca tcagacctca aggttaacaa catctgtata aatcggcgtt tatctgtggc
     2941 tgactccatt taaacgacgc aggttacggc tttgccgccg gatgtgaggc gcggctcgcc
     3001 gcgccgaggt agcccagaat atccgcgggc tatgcccttg gttagggctc aatccttaac
     3061 cttattgggc caaaggccgc accactgggc atagcccagc atgtagaggg caccgctccg
     3121 gcggggcata gccaccgcct cacttccggc ggcaaagccg aaacggacat gactcatgac
     3181 ttatgaggca aagccgttcc gtattaaaaa gaagaggctc gcacgacgaa gcgggcgagc
     3241 ccttggtaac tccagcaaca aggaatcttc agttaatcgt aatgtgtggt gccgtggctg
     3301 caggtctact tacgaatgtt gctccacctc tggtgatggt cactattatg ggaacatcac
     3361 cggcgccagc caatgaagcc ggcagcctga actggatcag atcccaaccg ggcatctccc
     3421 gattcggact gacgagcaca attgagtcgc cagtgatgtc tgtcgtagct gtagcggtta
     3481 cgatcgaaac cttcacttca gcagcagtcg cagttctgac cccggttaaa ttcacttcca
     3541 gcacagtggc gacggtgttg ccactgccgt ctgtcgacgt gacgctaaaa ggctctggcg
     3601 tccgcgccat gggattggtt acgttgaaga cagcagcgcg cccaccagcg tcattcgtgg
     3661 tcgtaaagat atccggttgc gcggggacga ttagcaatga acctcggaac tgaataccgc
     3721 cagccattct gctatggacg actaccgttt taactcccgc cgtcattcca atcggaacca
     3781 caaaattaat ttgcttcgat gcttgaccaa cgaaatacaa gcccgccgct gctccgttga
     3841 ctgccacaga cactccgttt agttccactg gaagtgccgg acttcgtttc gtttcggatc
     3901 ccccagttgc cgtggcgtct gcggccacga attccacgct ggtgcgaatg atgcccaact
     3961 cgcccggagc taaacctgta gccgtgcccg ggcttgggga gggcgttggc gtcggagatg
     4021 gtgacggact cgggctggct tcaggtagcg caacgttgct ggcgccagtg aagaaggaca
     4081 gcgtgccggc ggattcagta gtcacgggag gactcaacaa atagaagact tcggttgagc
     4141 catccggatt gccaccgccc agttcggcac ctccggcgct gaacacagtg cgttcgatag
     4201 tcgcagttgt gagcggacta aaggcagcaa aaactgaatt cttcgtcagc cgtctaaaag
     4261 tatttgttgt ggtgattggc agcgaggtag aaaaaacctg aaccgcccgc gaaggattca
     4321 aacctgtgct gtcttctgct gccggcggga aagtgccatc aaccttaaaa ttaagtgctg
     4381 atgcgtagat caacgtcgca ggtgccagcg atgaattgta atcggtgaac gttggaaaat
     4441 ggatcacgtc gcccgggaag ccaatcgctc gtttacctac aaggtcaaac ttgtcctggg
     4501 cgatcgtgta aacaaacacc gcgaggaaag gttgattcgt tgtggcattc gccttgggat
     4561 catctgcgag cgattcaaaa gccacgagcg tgccgtcacg actcaagcgc cggcccggac
     4621 tcaatacatt tacggtcgcg ccgaaagccg ggccactggc atcagccttg gttcgcgtgg
     4681 cctgccttgt aacagtgaac gtcgcgccgt tgaacgtgcc tatgtacacc tctgcatttc
     4741 cgtgtccgtt accgtcgtca ttgttattgt tgccgctgag gtttccattg gagatgaagg
     4801 cgacaacagt tccgtctgct gatagcgacg gcgaattctg aaacacggaa aacagcacag
     4861 gtcccacaaa gctgtctgcg gtcgccgtgc cctgcacgaa aactcccgga gcggaaatgg
     4921 tatgtaaaaa caattcggga ttcccgtcag cattattgga cgcaagattc cgcgtagaaa
     4981 tgaaggcgat gatctcgccg gtttccgaga tcgtcgcatc gcgattatcg tccgccacaa
     5041 aaggaaagat ccccgtagct cccggagacg gaacccggct cgccggagtg tcagtaatct
     5101 ttgtgaacgt tcccagggtc aaatcctgaa acggcaagtc ggcaccggtc gtaagatcga
     5161 cttcggtaat aatcggtaac tcgtaaatcc aaacttcctg attgccgtcc gcggctagca
     5221 cgccaccggt atcaacgcca tcgaataaac ccggatgcgg cgcattggcg ctgaagacaa
     5281 tgcggtaatg tccattggta cccggcacca gagtgatcat gggacgattg ttactgatct
     5341 cgatctgaat gtttgccgga tccggtggag tcggaactgg agtcggtgtc ggcgtgggcg
     5401 ttggtgtcgg actgggattc ggtgttggcg tcggtgtcgg agttggagtc ggagtcggcg
     5461 tgggcgtcgc cttggggaca ctcttggtat tggttatctg aaagatgcga cgctgcgcgt
     5521 aatcaatcag gaagatctcg cggttgccat cctcattgtt acggggattg ggatttagat
     5581 ttccggcgga gtcccggttc ggtatcttct cagtagcaat gtcgccattc gactcgacca
     5641 ccacgaaacg accatttgca gtgacgtctt tcgcgaaaga attgaatccg gcagctcccg
     5701 atgttaattg cgagacaaag ggttcagggg tggcgctcgg tgtttgcgct aaggcagaaa
     5761 aaaacacgcc tgaggcgagc gtaagcacaa tcagggcgca caacattctc agaactagct
     5821 tcacgaaatc ctccggaata aagacgcgac ttggagcgga ttagatgccc atgggagctt
     5881 gagcagcagt ccagcgccag gtaaattatt gaaaaatatt tcccttaaac actggccgtt
     5941 aaaagaatcg ctattctacg cagaacgccc gcttatccgc aagcgcaccc tcagttgcgc
     6001 ttccagttgt gccgtgatga gttaagtcac gcacgtttgc cccactaaga ggtctaaatc
     6061 cgctgacttt acggcatagt cgagaacagt tatcgttcaa cacatgacca ctgcattgcg
     6121 cgtcgaaggc gtttccaagc gctatcgtat ctacgagcgc ccgaatgatc gcctcagaga
     6181 aatgctgacg cgcggtcgtt ggaaacgcca tcaggagttc tgggcgctca aggatgtgag
     6241 ctttgaaatt gaagccggaa ctacaactgg catcatcgga ccgaacggca gcggcaaatc
     6301 taccctgctg caaataatga ccggcacgct cgagcccaca cacggaagtg tctggcacga
     6361 aggccggatt gccgcgttac tggaactcgg ttccggattc aatactgagt tcactggtat
     6421 tgaaaacgtg ttcatgaatg cttcgttgat gggtttttcg cgaagcgaga ctgaagcctt
     6481 gttgcctgaa atcgagcgct ttgcggagat cgggcccttc atctatcagc cagtcaagac
     6541 atactcgagc ggaatgtacg tgcgcctcgc cttttccgta gcgatcagtg cggccccaca
     6601 gatcctgatt atcgatgaag cgctggcggt cggcgatgct gttttccagc atcgttgcat
     6661 gcgccgcctg aaagagatgc aggagagcgg cgtaacgatc ttttttgttt cgcacgatct
     6721 ggcttcggtg cgagcactct gttcgcgtgc g
//
LOCUS       DASUEX010000177         3492 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1403752, whole genome shotgun sequence.
ACCESSION   DASUEX010000177 DASUEX010000000
VERSION     DASUEX010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3492
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1403752"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="VFV61_02930"
     CDS             <1..525
                     /locus_tag="VFV61_02930"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX4899456.1"
                     /translation="LPGMSGTELCRRFRREPLTRRTPIIMLTAKGSESDRVAGLDLGA
                     DDYITKPFSVRELLARVRAVLRRLDENTSQNYEDDQLAIDFADIRTVCNGRKIKLTNK
                     EFSLLSVLAKSADRVVTRQQLLDSVWGYSYYGDARTLDVHIRRLRQKLEECGDCIETV
                     VGVGYRFTGCARKT"
     gene            548..1822
                     /locus_tag="VFV61_02935"
     CDS             548..1822
                     /locus_tag="VFV61_02935"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX4899457.1"
                     /translation="MIRYSILLLALACLLAAFILLFLSFSVSAVVLAGFSFLAGFLLF
                     RKSVKKSDASVTHKLNSDTGTEIFALGKLFEATLGGMREGLLVVDSDLRVVASNPAAY
                     RLFNLASGKLDSRRLTELTRNPAIYNAFLEALQGKELSGVKVETHDPGRRVFDLRVVP
                     LGVAGRKNSAGAIGVFFDITQLEKLERVRQEFLSNVSHELRTPLTAILAFVETLEAGA
                     LNDPENSQRFLAIIRKNAGRMQNLIDDILELSAIESGTTGVQSQMIDLRPLANDVITS
                     LAAQATSQNVTVGNEVSAETTVFADPRRLEQMLTNLVENAIKFNRDGGEVFIECENGS
                     RSIIRVRDTGEGIPAQHLERLFERFYRVDRARSRDLGGTGLGLAIVKHLARAHGGEVR
                     VRSELGQGSVFEIDLPLKPEGNVAWVSSDDVN"
     gene            complement(1819..2061)
                     /locus_tag="VFV61_02940"
     CDS             complement(1819..2061)
                     /locus_tag="VFV61_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899458.1"
                     /translation="MKTLSPEIVNLIKYHLADCDFCNAEVPLLAHYHPPVKGECKAPE
                     IPINLRILAESLLGKKARERRKSENESLNYGLVAEG"
     gene            2738..>3492
                     /locus_tag="VFV61_02945"
     CDS             2738..>3492
                     /locus_tag="VFV61_02945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899459.1"
                     /translation="MKSTTSEIEKETEISGTEQTRPRTRRPNERQTKSGKWVESLRLS
                     DVGRLWMELHRVVSHHPLVRASRSAGLLIEEGSSTAYTDLTQELFVQLLSKNRFEHYL
                     ESAMTDYEIECEISQIELTNLLTAELRKRHPESYRLARRISTLIQSSSNFRRFDSSGI
                     DDEPHRRLADRVYGLSEWPDGKRRRDSHELEQRTHMVSVRSRDTRMVGCTGDSQVVIS
                     NPDLEDLIVSVLDAIDSPADVRTLRSLVMSRLPV"
BASE COUNT          794 a          922 c          954 g          822 t
ORIGIN      
        1 ctgccgggta tgagtggcac cgagctctgt cgccgttttc gccgcgagcc actaacgcga
       61 cgcacgccga ttattatgtt gacggccaaa ggttcggaat cggatcgtgt tgccggattg
      121 gatctggggg ccgacgacta catcaccaaa cccttttcgg ttagggaact gctggcccgt
      181 gttcgtgccg tgcttcgccg ccttgacgaa aacacttccc aaaactatga agacgatcaa
      241 cttgccatcg actttgcaga tatacgcacc gtctgcaatg gtcgcaagat caagctgaca
      301 aataaggagt ttagtttgtt gtcggttctg gcgaagagcg cagatcgggt ggtgacacgg
      361 cagcagcttc tggatagcgt ctggggctac agttattatg gtgacgcgcg gactcttgat
      421 gtgcacatta gacgcttgcg tcagaagctg gaagagtgcg gcgactgcat cgagacggtg
      481 gtcggcgtcg gttatcgctt cacgggatgt gcccgcaaga cgtaggctta agatctaagg
      541 ttgtaaggtg atcagatatt ccattctctt gcttgctctt gcgtgtttgc tggctgcgtt
      601 catcctcctc ttcctttcct tcagtgtcag cgcagtcgtg cttgctggtt ttagcttcct
      661 cgccggcttc ctcctctttc gtaagtctgt caaaaaatcc gacgcttccg taacccacaa
      721 gctgaattcc gacaccggga ctgaaatctt tgccttggga aaattattcg aagcgaccct
      781 cgggggcatg cgggaaggac tgttggttgt cgacagcgat cttcgtgtcg tcgcttcgaa
      841 cccggcagca taccggcttt tcaatcttgc ctctggcaag cttgattcgc ggcgccttac
      901 ggaattgacg cgcaacccgg cgatttacaa cgcctttctc gaagcccttc agggaaagga
      961 actttcggga gtgaaagttg agacgcacga ccccggacgc cgtgtttttg atttgcgggt
     1021 tgtacccctg ggcgtcgccg gccgaaaaaa ctcagccggc gcaatcggtg tgttttttga
     1081 catcacacaa ttggaaaagc tggaacgggt gcgtcaggag tttctatcca atgtgtctca
     1141 tgaactgagg actcccttga ccgcaatcct ggcgtttgtg gaaacgctgg aagcgggagc
     1201 gctcaacgat ccggaaaata gccagcggtt tttggccatt attcgcaaga acgctggacg
     1261 catgcagaac ctgatcgatg acattttgga gctcagtgcg atcgagagcg ggacaaccgg
     1321 ggttcagtcg caaatgatcg acctacgccc gttggccaat gacgttatta catctctcgc
     1381 tgcccaggca acgagccaga acgtgacggt cggcaatgag gtgagcgcag agacaacggt
     1441 ctttgcagat ccgaggcggt tggaacaaat gctgacgaac ctggttgaga atgcaattaa
     1501 gttcaatcgc gacgggggcg aagtcttcat tgaatgcgaa aacgggagcc gcagtattat
     1561 tcgagtgcga gacactggcg aggggattcc cgcgcagcac ctggagagac tcttcgagag
     1621 gttctatcga gttgatcgag cgcgctctcg cgacctcggg gggactggac taggcctggc
     1681 tatagtcaag cacctggcgc gagcgcatgg cggcgaagtt cgcgtcagat ccgaactggg
     1741 tcagggaagt gtgtttgaaa ttgacctgcc gttgaagccg gaggggaatg tcgcgtgggt
     1801 gagttcagat gacgttaatt agccttcggc caccagccca taattgagcg actcgttctc
     1861 tgatttgcgg cgttcgcgcg ctttctttcc cagaagggat tcggcgagga ttctcaggtt
     1921 gatcggaatt tcgggtgctt tgcactcacc tttgactggt gggtggtagt gagccaacag
     1981 aggaacctca gcgttacaga aatcacagtc cgccagatga tatttgatca ggttcacgat
     2041 ctcgggtgaa agggttttca tacgaaacga caaaaggata tttgacgaag gacaactcgg
     2101 cgctttcgtg aatgaagtag cgttagaaac tctcatttag acccccaaaa tgtcgcgctg
     2161 acaccggggc ttccttttta gggcgatttc ccaaaataac tccgcagaag cgtcctggat
     2221 gagaggccag aggagcacgt ggccatcaaa actgctgccc gctcggttat caacctttga
     2281 ataggattaa tcccctcatt gttccagact tttggagcct ctttgagtta cttttctcta
     2341 ttgttttcag cgattttgcc ccggtgtaaa aacttgttgc ccggttgctg gattcttgct
     2401 actatgacac ccgcttaagg caaagagcgc ctgctctttg accccttgcc aggaagaaaa
     2461 ctcccttcgt cttcgactca aaatttgagc agaacctaga ttgtcgagat aaaagactag
     2521 ctccctcccc atcggacgag cgactggcta atccttaagc acgcccctca atcaatagaa
     2581 agattgcccc tggtcctccg aaaaacccat atccaggtga gaaaaacctc tgggatgaca
     2641 ccgaaaacga gatcaccacg atagtttcag ccttctggca gcccctaccc accgccctta
     2701 aaagcctatt ccaatcaccg cgtcaacgga caatcctgtg aaatccacaa cttccgaaat
     2761 cgaaaaagag accgagatca gcggaaccga acaaactcgg cctcggacgc gccggcctaa
     2821 tgaacgtcag acgaagagtg gcaagtgggt tgagagtctg cgcttgtctg acgtgggacg
     2881 cctctggatg gaactccacc gcgtcgtctc tcaccatccg ctcgtaagag cctctcggag
     2941 tgcaggactg ctgattgaag agggcagctc cactgcatac accgacttga ctcaggagtt
     3001 gttcgtgcaa ttgctcagca agaaccgctt cgagcactac ctcgagtcag cgatgactga
     3061 ctacgaaatc gagtgtgaga ttagtcagat tgagctgacg aacttgttga ctgccgagtt
     3121 aagaaaacgg catcctgaaa gttatcgcct ggcgcgccgc atttctacgc tcatccaatc
     3181 cagttccaac tttcgccgct tcgattcaag cgggattgat gacgagccgc atcgacgtct
     3241 ggccgatcgc gtttatggtt taagcgagtg gcccgatggc aagcgtcgcc gcgattcgca
     3301 cgaactcgaa caacggacac acatggtttc cgtccgctcg cgcgacaccc ggatggtcgg
     3361 ctgcaccggc gattcccagg tcgtgattag taatccggac ctggaagact taatcgtttc
     3421 cgttttggat gccatcgatt ccccggccga tgtgcgcacc ctgcgaagcc tcgtaatgtc
     3481 gcgcctgccg gt
//
LOCUS       DASUEX010000178         5257 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_36289, whole genome shotgun sequence.
ACCESSION   DASUEX010000178 DASUEX010000000
VERSION     DASUEX010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5257
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36289"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            271..636
                     /locus_tag="VFV61_02950"
     CDS             271..636
                     /locus_tag="VFV61_02950"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX4899460.1"
                     /translation="MKYMLLVHHDEITFAERSETERQQLIQESVQLANHLHSEGKYLN
                     AAPLHPTAETTCVKVRDGKLFVTDGPFAETREQLGGFFLIDAEDLEEAIGIAARIPGA
                     RIGTVEVRAVTEVEGLPQD"
     gene            complement(753..1346)
                     /locus_tag="VFV61_02955"
     CDS             complement(753..1346)
                     /locus_tag="VFV61_02955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899461.1"
                     /translation="MTTAAYFIVIMIIATALYFGIRWLVRASSQYRGVKVVTCPETER
                     PAQVEVDARHALLTAFVGRPDIRLENCSRWPIKEQCGQECLTNLDVAPSECLVSGVLM
                     RWYRGKKCVYCDKSGEELHWMDHKPALQTPDGKLVEWRDVPIEDISMVLKTHLPVCWN
                     CYIAQSFRKDHPDLVTYRPWRQSTPGDADGLSESRHL"
     gene            complement(1349..1747)
                     /locus_tag="VFV61_02960"
     CDS             complement(1349..1747)
                     /locus_tag="VFV61_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899462.1"
                     /translation="MPKDDRDILEILKDELSFIEKGGYGRSVRTPWQPKSVFQDSLAC
                     INYADPNHTHPCNECRLIDFVSPQHKREAIPCHFIPLDEAGETVEDMELEDNEAKLER
                     KVKDWLRARIREIEEERSRISQRSTEELRG"
     gene            complement(1859..2047)
                     /locus_tag="VFV61_02965"
     CDS             complement(1859..2047)
                     /locus_tag="VFV61_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899463.1"
                     /translation="MREIPQSYVRRYREWARGMPLGDRRLALAMSSFGLSILAHETAK
                     FSQPDRSPSANFLTILSG"
     gene            2181..2915
                     /locus_tag="VFV61_02970"
     CDS             2181..2915
                     /locus_tag="VFV61_02970"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX4899464.1"
                     /translation="MQNATNLDVETEGNGGRPLPDEALLQLFHGSDGIKLTSIHPRGA
                     VLFAEGQPGDGVYLLRTGRAKVSISSAAGKTVILRVAQPGALLGVNSVIKDVPHEVTV
                     ETLERCRIDFISRSDFIKALAKTDPALVGVAHTLGNELSEVFNHLRSLLLSQSAAERL
                     ARLLLKWCDEEGESVSEGVRVNSGLTHEEISQIICTSRETVTRLFAELRKKHNVGFAG
                     NAIFVRDRRALEALVCSREIAGISSG"
     gene            complement(3173..3334)
                     /locus_tag="VFV61_02975"
     CDS             complement(3173..3334)
                     /locus_tag="VFV61_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899465.1"
                     /translation="MRGMSKGFELILVTIFANVAAKVVIGARSYRASFARFNRLRRTA
                     GCEPHDSCS"
     gene            complement(3344..3745)
                     /locus_tag="VFV61_02980"
     CDS             complement(3344..3745)
                     /locus_tag="VFV61_02980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899466.1"
                     /translation="MEGQIKLVLHLGVAAQAKLWFAAFQKFDGREARLFGVCRRYESD
                     RAGNIPSAGAAMGGVAICTTYVVAPVFSALEIIVLFSAGMARKTSLRYFFGRLVFEGN
                     NLRRIAFFDVRSAGTMTRLATCHLPLPTANG"
     gene            4190..>5257
                     /locus_tag="VFV61_02985"
     CDS             4190..>5257
                     /locus_tag="VFV61_02985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899467.1"
                     /translation="MKSKTKSKFATKVLVWLALVIFTSVAIAQQPSPTPKKSPEPQPT
                     VDAGEDAGNYTVVGSIEFGYRGLSVDGDHNKYQSDLNYRAGPRLFDSTFLLKSKDGKG
                     GLFDTLLVTSTGWGGDPNGNVRMSVEKAKWYRFDGTYRRFKYFRFLNSFANPNWVFSP
                     ATFSRPPNPISGEHGYDTRMQLGDFDLTLLPKDERIRFNIGYSPERYDGPAFTNYHVG
                     GNEFNLLQQMKSRANDFRVGADGKVGPIDFSFLQGFRRFRDDTSIHLGTTPGINLNPT
                     AASLTSFHRDEPARGKVNYTRLSLHSLIAKKLDITGRIIYSKATSNSSLAESFTGRNW
                     NPRVTGWPPTPPAATPNILNLG"
BASE COUNT         1277 a         1406 c         1395 g         1179 t
ORIGIN      
        1 aagaagcgga gaaactcttc aacggactct ccgccggcgg gaaaattgaa atgccgctgc
       61 aggacatgtt ttggggagcg tattatggtt cattcgctga caaattcggc gtgcagtgga
      121 tggtgaacta cgacaagaat ggccggcagt gattgtcgga atgttaaaaa gcgcttcctg
      181 tcgtgtccga aactctttgc cgctccggca gcgtgagggt ttattagaaa tgcagaaagt
      241 actctcaaga caaagaaaga ggaaggccct atgaaataca tgctgctggt tcaccacgac
      301 gagatcacat ttgcagagcg cagtgagaca gagcggcagc aattgattca ggagtctgtc
      361 caactggcga accacctcca ctcagagggg aaatacctga acgcggcacc gctgcatccg
      421 acggcggaga caacctgcgt caaggttcgc gacggtaagc ttttcgtaac ggatggtccg
      481 ttcgccgaga cacgagagca actgggagga ttctttctga tcgacgctga ggatctcgaa
      541 gaagcgattg gcatcgccgc ccgcattccc ggagcgcgta tcggaactgt cgaagtccga
      601 gcagtaactg aggtggaggg cctgccgcag gattaattaa tgtaaggcgt ccctccgtgg
      661 gcagcccgct tagaccaagg gcggccatgg aggccgcccc tgcaagaaac caaatcagct
      721 agaaaaccaa cccacccgtg cagccttacc aatcaaaggt gacgtgactc agacaagccg
      781 tctgcgtctc caggagtgct ctgccgccat ggtctatagg tgaccaggtc gggatgatcc
      841 ttgcgaaacg actgcgctat gtaacagttc caacaaaccg gtaggtgcgt cttcagcacc
      901 atcgagatgt cctcaatagg aacgtcacgc cattccacca gcttgccgtc tggggtttgc
      961 aaggctggtt tatggtccat ccaatgaagt tcctcgcccg atttatcaca gtagacgcat
     1021 ttctttccgc ggtaccatct cattagcacg ccgctaacta agcattcact gggcgctacg
     1081 tcgagatttg taaggcactc ctgtccgcac tgctctttga tcggccagcg cgagcaattc
     1141 tccagtcgaa tatccggacg gcccacgaac gcagttaata acgcgtgacg cgcgtccact
     1201 tcgacctgtg ccggcctttc tgtttcggga caagtgacta ccttgacacc gcgatattgg
     1261 gacgaagctc tgaccagcca acggataccg aagtagaggg ccgtagcgat aatcatgatt
     1321 actatgaagt aagctgcggt agtcatgttt aacctcttaa ttcctcggtc gatcgctggc
     1381 tgatccttga gcgttcctcc tcaatctcac gaatccgagc acgaagccaa tccttaactt
     1441 ttcgctcgag ttttgcttcg ttgtcctcaa gctccatatc ttcgacagtc tcacccgctt
     1501 catccaaagg aatgaagtgg caaggtattg cttcgcgttt gtgttgcgga ctgacgaagt
     1561 cgatcagacg acattcgttg cacgggtggg tatggttggg atcagcgtag ttgatacagg
     1621 ccagagaatc ttgaaaaacc gatttcggct gccatggtgt acggactgac cgtccatacc
     1681 cgcctttttc gatgaagctc agttcatctt taaggatttc gagaatgtcg cggtcgtctt
     1741 ttggcatggc ctaaaacctc catgatcccc ggcctaaccc aagtagcctg ccgctgcttt
     1801 tgctgagcca atcacactgt cccaaccact ctatgggacg gcaatgcata tgccggaact
     1861 acccgctgag aattgttaag aaatttgcgc tgggggaacg atcaggctgg gaaaacttcg
     1921 cagtctcgtg cgcgagaatg ctcagtccga aactcgacat tgccaacgcc aggcgccgat
     1981 cgccaagcgg cataccacgg gcccattcgc ggtagcggcg aacatagctt tgcggaatct
     2041 cccgcaatag gcgacccgat tgcgcaatat tccgccagag acatcgctaa agaatactgt
     2101 tggcggcatt aacaactgag attcaggctg gcacaagcat tgcgaattcg tccgcgctgg
     2161 cagtcttaac agggggaccg atgcaaaacg cgaccaatct cgatgttgaa accgaaggaa
     2221 acggtgggcg cccattacct gacgaagccc tgctccagct tttccatggt agcgacggca
     2281 tcaagctgac tagcatccat ccgcgcgggg cggtgttgtt tgccgaaggt caaccaggtg
     2341 atggcgttta cctgctgcgc acgggtcgcg ccaaggtttc aatctcttcc gctgccggga
     2401 aaacggtaat tctacgggta gctcagcccg gcgctttgtt gggtgtcaac tccgtgatca
     2461 aggatgtacc acacgaagtt acagttgaaa ccttagagcg ttgccggatc gactttatct
     2521 cgcgatccga tttcataaag gcgctcgcca aaaccgatcc cgcgcttgtc ggcgtggctc
     2581 ataccctggg caatgaattg agcgaggtgt tcaaccacct caggtcatta ctattgtctc
     2641 agtctgctgc ggagagactc gccaggctgc tgctcaagtg gtgcgatgag gagggtgagt
     2701 cagtatccga aggtgttcgg gtgaactctg gactgacgca cgaagagatt tcccagatta
     2761 tctgcacatc gcgcgagacc gtgacgcgcc tatttgcaga actgagaaag aaacacaacg
     2821 taggttttgc cgggaacgcg attttcgttc gcgaccggag ggcgcttgag gccctcgttt
     2881 gctcccgaga aatagcggga atttcttcgg gttgatgtga tggtcgtcac agccagatat
     2941 cgcctcgacc attacaatcg cttcggattg acagcgaaag agtggtatat aggaaggcta
     3001 aaggctgagc tcgtctgagt cgaattcttg tgagcccggt cgtacccgcc ggctcccgaa
     3061 gttgtaggaa ctatccgatc ttttaaaccc gattgcgatc cactgcgatt gcgacaggga
     3121 ggtcattgtg agtctaaaaa gaaccatcca ggtttttctc gtctgcggat tcctagctgc
     3181 agctatcatg tggctcgcat ccggcagttc ttctcaatct attgaacctg gcaaagctgg
     3241 cgcggtagct gcgcgcgccg atgactacct tggcagcgac gtttgcaaag attgtcacga
     3301 ggatcagttc aaatcctttg ctcatacctc tcatgctgcc ctctcaacca ttggcagttg
     3361 gaaggggaag gtgacaggtt gcgagtcttg tcatggtccc ggcagagcgc acgtcgaaga
     3421 aggcgatccg acgaagatta tttccttcaa aaacaagtcg cccaaagaaa tatcggagac
     3481 ttgtcttacg tgccatgccg gcagagaaga gcacaataat ttcaagcgcg gagaacactg
     3541 gcgcaacgac ataggttgtg cagattgcca ctccgcccat ggcagcccca gcggaaggaa
     3601 tgttgccagc tcgatcactt tcatatcgcc ggcaaactcc gaaaagccgg gcttctcgac
     3661 cgtcaaactt ctgaaaagca gcgaaccaca gctttgcttg agctgccaca ccgaggtgaa
     3721 gcaccagttt aatttgccct tccatcacaa ggtgctggaa ggcgccatga agtgcagtga
     3781 ttgtcataac cctcatggcg gttttgaact aaaacaaaca agactcgcga ctggcgccga
     3841 cgcggcttgc ataaagtgtc atgcagataa acagggtcct tttgtttacg aacatgcccc
     3901 ggtgaaaacc gaagggtgcg tggcgtgtca tacaccacac ggctctgcga atccacggct
     3961 gttgtcaact agcagggtgg cccagctttg tctgacatgt cacagtgttg cccacgacgt
     4021 tggcgcaatt gagccggccg gtccgcaaca taacctgaac gcgcaatacg ccaattgcac
     4081 aaattgtcac gtaaagatcc acgggtcgcg cactagtccc gtcttcttca gatgaggcct
     4141 ttgcagacgg agttgaaaag ccgtttgcat tttgatccag agaatagtga tgaagagcaa
     4201 aaccaaatcg aaattcgcca caaaggttct ggtctggttg gcgttggtga tcttcacgtc
     4261 agtagcaatc gcccaacaac cgagcccgac gccgaaaaaa tctcctgaac ctcaacccac
     4321 tgtcgacgcc ggagaagatg ctggcaacta cacggtcgtc ggcagcatag agtttggtta
     4381 tcgcggtctg agtgtggacg gcgaccataa caagtaccaa agcgatctca actacagagc
     4441 cgggccgcgg cttttcgaca gcactttcct gttgaaatcc aaggacggca agggcggcct
     4501 cttcgatacc cttttggtca cttcgacggg gtggggtgga gatcccaacg gtaacgtgcg
     4561 aatgagtgtc gaaaaagcga aatggtatcg ctttgacgga acctatcgcc gcttcaagta
     4621 tttccgattt ctgaacagtt tcgcgaatcc gaattgggtt tttagtcctg ctactttcag
     4681 tcggccgcct aatccaatta gtggcgagca cggttacgat acgcgcatgc agttgggaga
     4741 tttcgatctc actctgctac ccaaagacga acgcatcagg ttcaacatcg gctattctcc
     4801 ggaacgttac gacggtcccg cattcaccaa ctaccatgtc ggtggaaacg agtttaatct
     4861 gctgcaacag atgaaatccc gtgcgaacga ctttcgcgtc ggcgcggacg gcaaagtcgg
     4921 gccaattgat ttttcgtttt tgcagggctt tcgccgcttt cgcgacgata cgtcaatcca
     4981 cctgggaaca acgcccggga taaatctaaa tccaacagca gcgagtctga cgagctttca
     5041 tcgtgatgaa cccgcacgcg gaaaagtcaa ctacacacga ctaagcctgc attcgctgat
     5101 agcgaaaaag ttggatatta ctggacgtat tatttacagc aaggcaacat cgaactcctc
     5161 gttggcagaa agcttcacgg gtaggaactg gaaccccagg gtgactggat ggcctccgac
     5221 tcccccagcg gctaccccga atatccttaa cctcggc
//
LOCUS       DASUEX010000179         2757 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_608007, whole genome shotgun sequence.
ACCESSION   DASUEX010000179 DASUEX010000000
VERSION     DASUEX010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_608007"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..731
                     /locus_tag="VFV61_02990"
     CDS             <1..731
                     /locus_tag="VFV61_02990"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEX4899468.1"
                     /translation="LVSEVAPDQTARLTISRGGSEQEVAVTIAKRKDSDALTRIQGFG
                     KGLEGFKNFEGLKPGAQVWKWEGDGDGPLVLAFGNNRRIGVSTTTLTKQLAEYFGIVD
                     GKGVLVTSVTEDSAAAKAGIKAGDIITAIDGEKIEDAGDLARGINKKKEGDVTLTVIR
                     NKNQRNITVTPKTVSNPMGMPGTAPQIGRRVIVPRIELPVIPEMNISIPRIELPAIPE
                     INVEFPARAPRPRIRRLPREQHPI"
     gene            complement(898..>2757)
                     /locus_tag="VFV61_02995"
     CDS             complement(898..>2757)
                     /locus_tag="VFV61_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899469.1"
                     /translation="STSSPSLFQSFDFMKRYLNILFAFALVASVAAGNVLAQLPQIDS
                     STLPQAQQRSTSGFNLAEYGVEVQPDSRLIIMMAALEAAGFDPTPAGREPSAFRAQVR
                     RDLAGLDPDLRQRLRAFYERNKLPAPATAADQAARYVSLAFAFTAMPLFEAPARSEDL
                     PGGLLEVLDFAPLVREFYRKSGIDERLVSYTRAYQAEGDRLRQPAAIMVSAVLSYLHT
                     RPITITNERVISKSPGKKTTAKAYTMRSHERRFLIVPDLLAAPGVINLRVIGDDYYAV
                     LPEGTNPQSSELQRAYLQYVVDPLMLRFNRSIADRREQIRQVIADREKAGGTVTPDVF
                     LVVSRSLVAAADARFEEVKRLDALSREARRKLDQTKDESLKASIARETQAEMNAIRDE
                     TIAKLADEYERGAVLAFLFADELKGIESSGFDVANFFVDIITSFDPARESRRPAEYAE
                     ARQRALAARQARLAARNAETETQAYSPAEASRAAALVKQLSQVEELLRLKDYPTAETR
                     LKDLIKDNPREPRIFFALAQTASLAAGDATDEEVQAERLNRALANYRLAVQAAAPETD
                     RGLMSRAHEAMGRIHAFLENPAEAAKEFDEAIKLGEVRGGAYKEAVENRKKLP"
BASE COUNT          626 a          767 c          799 g          565 t
ORIGIN      
        1 gcctcgtgtc agaagtggca cccgatcaaa ctgcgcgctt gacgataagt cggggcgggt
       61 cggaacagga agtggcagtc accattgcca aacgcaaaga ctccgacgcg ctcactcgca
      121 tccagggatt cggtaaaggc ctcgaaggtt ttaagaattt cgaaggactt aagccgggag
      181 cgcaagtttg gaaatgggag ggcgacggcg atggtcctct ggtgctggct tttggcaaca
      241 accgccggat cggcgttagc acgacgacgc tcacaaaaca gcttgccgag tactttggca
      301 tagttgacgg caaaggcgtt ctggtaacgt cggttaccga ggatagcgcg gcggctaagg
      361 cgggaatcaa ggccggcgac atcatcacgg ctatcgacgg tgagaagatt gaggacgccg
      421 gcgacctcgc acgcggaatc aacaagaaga aggaaggcga tgtgacgctg accgtgattc
      481 gcaacaagaa tcagcggaac atcaccgtca ctccgaagac agtttcgaat ccgatgggaa
      541 tgccgggaac ggcgccccaa atcggccggc gagtaatcgt cccgcggatc gagctgccag
      601 tgattccgga aatgaacata tcgattccgc gaattgaatt gccggcgatt ccagagatca
      661 acgttgagtt tccggctcgt gcgccacgac caaggattcg acggttgcca agagaacaac
      721 atcctatata acgcgaagtc gcagagacgc gaagtcgcag agacgcggag tcgcagagaa
      781 aacataaggc cggctagagg acctaacgac aggttcctca gccgaccttt tacttttgtt
      841 catctctgcg tctccgcgac tttgcgtctt tgcgaagaga acttgagacc tgagactcta
      901 aggcagcttc ttccggttct caaccgcttc cttgtatgca ccaccccgga cttcgccaag
      961 cttgattgct tcgtcaaact ccttggccgc ttcagctggg ttctctaaaa acgcgtggat
     1021 gcgacccatg gcttcgtggg cgcgagacat caagccgcga tctgtttccg gggcagcagc
     1081 ttgcaccgca agccggtaat ttgcgagggc gcgattcaac cgctcagcct gcacctcttc
     1141 atcggtcgca tctccggcgg ccagactcgc ggtctgtgca agcgcgaaaa agatccgcgg
     1201 ttcgcgcggg ttatccttga tcagatcttt caagcgcgtc tcggctgtcg gataatcttt
     1261 caaccgcagc agttcctcaa cctgcgaaag ttgctttacg agtgctgcgg ccctggatgc
     1321 ctctgcgggt gaataggctt gagtctctgt ttccgcattg cgcgccgcca ggcgcgcctg
     1381 ccgcgccgcg agtgcccgct ggcgtgcctc cgcatactcc gctggacggc gactctcgcg
     1441 ggcggggtcg aatgaagtaa taatgtcgac aaagaaattc gcgacgtcga aaccactgct
     1501 ttcgattcct ttgagttcat cggcaaacag aaacgccagc accgcaccac gctcgtactc
     1561 atcagcgagc ttggcaatag tctcgtcgcg gatggcattc atctccgctt gcgtctcgcg
     1621 tgcaatgctg gccttcaaac tctcgtcttt agtttgatcc agtttgcgac gcgcttcacg
     1681 agacagagca tcgagtcgtt taacttcctc aaaacgcgca tcagccgccg ccaccaggga
     1741 ccgcgagacg accagaaaga cgtcgggagt caccgtgcca ccggcctttt cacgatccgc
     1801 aatcacctgg cgaatctgtt cgcggcgatc ggcgatactg cgattgaagc gcagcattaa
     1861 cgggtcaact acatattgca gataggctcg ttgcagttcc gacgattgcg gattggtccc
     1921 ctccggcagg acggcatagt agtcgtcacc aatgacccgc aggttgatca cgcccggcgc
     1981 ggcgagcaag tcaggcacaa tcaaaaacct gcgctcgtgg gatcgcatgg tgtaagcctt
     2041 ggccgttgtt ttcttccctg gtgatttcga gatcacgcgt tcgttggtaa tcgtgattgg
     2101 gcgtgtatgc agatatgaaa gtacagcgct aaccatgatg gcagccggtt gtcgtaaacg
     2161 atcgccttcc gcttggtagg ctctcgtgta gctgaccagc cgttcgtcaa ttcctgactt
     2221 gcgatagaac tcgcgcacga gtggggcgaa atccagaacc tccaggagac caccgggaag
     2281 gtcctcagat cgtgctggcg cttcaaacaa aggcatagca gtgaacgcaa atgcgagtga
     2341 gacataacgc gctgcctggt ccgccgcggt agctggggcc gggagtttat tgcgttcgta
     2401 aaaagcgcgg aggcgctggc gcaaatcggg atcgaggcct gcaaggtcgc gtctcacttg
     2461 tgcgcgaaag gccgaaggtt ctcttcccgc tggcgtggga tcaaagccgg cagcttcaag
     2521 cgccgccatc atgatgatca gacgcgaatc gggttggacc tcgaccccgt actccgcgag
     2581 attaaaaccc gacgtggagc gctgttgcgc ctgtggcaaa gtgctggaat caatctgcgg
     2641 caattgtgcc aacacgttac cagccgcgac gctggcgacc agggcaaaag caaataagat
     2701 gtttagatat ctcttcataa agtcaaaaga ctgaaaaagt gacggggagg aggtaga
//
LOCUS       DASUEX010000180         4048 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_837554, whole genome shotgun sequence.
ACCESSION   DASUEX010000180 DASUEX010000000
VERSION     DASUEX010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4048
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_837554"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1794)
                     /locus_tag="VFV61_03000"
     CDS             complement(<1..1794)
                     /locus_tag="VFV61_03000"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX4899470.1"
                     /translation="MAGVPGKQRFILISLAVAVVLGATLPAFLTASCFTHQNTPAELK
                     ALENLRSQTRRGQLPAEEVVTRIEAEFPRTKAAGLARLVRARIKVRANDYAGAATLLD
                     TNVIQDYTSLGDHALFMRGNALEQTGQTAAARIAYERLIRSYPSSIRAREATLRVADI
                     LLKSGQGTAVPVLLKELTNKNDAAALLLLAKASAQSGDNARALEAYRQIYFFAPASSE
                     SAAAQTMIPQLSSTLSPLNEAEATARAEKLFDAKRYNDAYQAYTDALAKFPGLANTQN
                     QLRRGIAAANAKRTEAAAVLSNIPSSAGESRAEALFYLTQSYARARQWEQVRATVEEL
                     RRAFPNSVFLPRALVSAGQIAADAKNDADATYFLRAAVTAAPGSIEVVQAQFDLAWQA
                     HEARNFSESAKLLTEHLAYYVDKNNDNRGRAGYWAARDSERAGKLAEARALYQAVLGR
                     YDANWYGYLAKQRLNAMKGSTPAQGFPSDSVVGRAMANLQTVTVAEETAGAAEESRIM
                     KAEQLNNVGLDDWAFEELARAAESGPNSPKVNLAIAQIYRYQEDNVRALNTLKKSFPD
                     YSQMKPEEMTRAEWDVFYPLAYWDIIVQESRA"
     gene            complement(1922..3304)
                     /locus_tag="VFV61_03005"
     CDS             complement(1922..3304)
                     /locus_tag="VFV61_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899471.1"
                     /translation="MPTTRAMEETTVVSGKLEESLRHEIESYIEGRLGGVKQEIAELQ
                     SRLNEALTQLLDRQSEVQLDGSVAASIMEHLKAAHDEGIDLAASESARAKASSDMAIV
                     KAAVTEMGEQTSQGEILKTLVNRAASFAPRVAFFAVKGDNAIGWRARGFEGSVGDSAV
                     QQLSIPLSGDNLLSDVAKSRTTWSGGAHSHSENYVLLNRLGQEAPPERVVSIPLNVRK
                     RAVAVLYADSGSLDSEAINLEALETLVQMCGMCVDLLAAQAPAKAPAPQTEHQEATPE
                     PAVSSYAPEREYAPEPVAEAETAVAEVAAEVVPAEIVDEPQPTDFVADIVEAEAEAAP
                     EEVSPPVDALTPSVPRRRYGADVELPVAVSGEEERRLHVDARRFARLLVSEIKLYNEQ
                     KVQEGRMQSDLYDRLREYIDRSRDMYDKRVKPEVAQSYDYFHHELVNTLAEGDAGKLG
                     NAYPGATVSA"
     gene            3557..3976
                     /locus_tag="VFV61_03010"
     CDS             3557..3976
                     /locus_tag="VFV61_03010"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX4899472.1"
                     /translation="MAATILIADDYEDNLELLRLMLEAGSYRVREARDGRQCVTMALA
                     EPPDLVMIDLSMPLLDGWGVIEELQNDERTAGIPCVAVTAYGDSDRERALQTGFDAYI
                     AKPFRSAELLQVVASLIGTSKTKSSPQGLERADLPAL"
BASE COUNT          820 a         1151 c         1162 g          915 t
ORIGIN      
        1 cgcgcgcgat tcctgaacga tgatgtccca gtaggcgagc gggtaaaaca cgtcccattc
       61 ggcgcgcgtc atctcttcgg gcttcatctg cgaatagtca gggaagcttt tcttaagcgt
      121 gttcagcgcc cgcacattgt cttcctgata acgatagatt tgggcaatcg cgaggttaac
      181 cttcggacta tttggtccag actcagccgc ccgtgccagc tcttcaaatg cccaatcgtc
      241 gagcccgacg ttgttcagtt gctccgcctt catgatccgg gactcttccg cagcgccggc
      301 agtctcctcg gcgactgtta ctgtttgcag gtttgccatc gcgcgtccca ccacagaatc
      361 agaggggaag ccttgcgccg gcgtgctgcc cttcatcgca ttcaaacgct gcttcgcaag
      421 gtagccatac caattcgcgt cgtaacgtcc gagcacggcc tggtaaaggg cgcgggcttc
      481 ggcaagtttg ccggcacgtt cggaatcacg ggcagcccag taaccggcgc gaccacgatt
      541 gtcgttgttt ttgtcgacgt aatacgcgag atgttctgtg aggagttttg ctgactccga
      601 aaaatttcgc gcttcgtgag cctgccaggc gagatcaaac tgggcctgca ccacttcgat
      661 cgaaccgggc gctgcggtaa ccgcggcgcg caggaaataa gtcgcatcgg catcattctt
      721 agcgtctgcc gcgatttgtc cggcgctgac aagtgcacgc ggcagaaaaa cgctattggg
      781 aaatgcgcga cgtagctcct ccacggtcgc acgcacttgt tcccattgac gcgcgcgcgc
      841 gtacgattga gtcagataaa agagagcttc ggcgcgcgac tcaccggcag aggaaggaat
      901 gttactcagg actgccgcag cctccgtccg cttcgcgttc gccgcagcga tgccacgacg
      961 caactggttc tgggtattcg ccagaccggg aaacttcgcg agcgcatcgg tgtaagcctg
     1021 gtaagcgtcg ttataacgct tggcgtcaaa caatttctca gcacgagcgg tagcctcagc
     1081 ttcattgagc ggcgaaagcg tggacgagag ttgcggaatc attgtctgag ccgccgcgct
     1141 ttcggaggac gccggggcga agaaataaat ctgccgataa gcttctaacg cacgtgcatt
     1201 gtctccactc tgagccgaag ctttcgctaa taacagaagc gcggctgcgt cgtttttgtt
     1261 cgtcaattct ttcaggagga caggaaccgc tgtcccctgg cccgatttta agagaatgtc
     1321 agcgactcgc agtgtcgctt cacgagcgcg aatcgaactg ggatagcttc tgatcagtcg
     1381 ttcatacgct atgcgcgctg cggcagtctg tcctgtttgt tccaaagcgt tgccacgcat
     1441 gaacagggca tgatcgccaa gcgaggtgta gtcctgaatt acgttcgtgt cgaggagggt
     1501 cgccgcgccg gcatagtcgt ttgcgcgaac cttgatccgt gcgcgcacca ggcgtgctaa
     1561 acccgctgcc ttggtacgcg gaaactccgc ctcgatgcgc gtaaccacct cttccgccgg
     1621 taactgaccg cggcgggttt gcgaacgtag attctcgagg gcctttaatt cagcgggagt
     1681 gttctggtgg gtaaaacaac tggcggtcag aaaggccgga agcgtagcgc ccaaaactac
     1741 ggctaccgcc agcgaaatga gaatgaaacg ctgcttgcca ggtacacctg ccatgttttt
     1801 cccccaaaga agtcaggctg ggaaaaccag cctgaagttt aaaaaagaag agtcccaagt
     1861 ctcaaggccg cttgcaaaga ctttggtctt gagacttgag acttaaaacc tgagacggat
     1921 tttaggctga aacagttgct ccgggatagg cgttgcccag tttcccggcg tccccttcgg
     1981 cgagggtgtt taccagctca tggtggaagt agtcataact ctgggccact tccggtttca
     2041 cacgtttgtc atacatgtcg cgtgagcgat cgatgtattc ccgcaggcgg tcgtaaaggt
     2101 cactctgcat gcggccttcc tgaactttct gttcgttata gagcttgatc tcggaaacaa
     2161 gcagccgggc aaagcgtcgg gcatcgacat gcagtcgccg ttcttcctcg ccggaaacgg
     2221 cgaccggaag ttcaacgtca gcgccataac gtcgccgcgg cacgctcggc gtcagggcat
     2281 caactggagg tgagacttct tccggagccg cttcggcttc agcttcaacg atgtccgcaa
     2341 cgaagtctgt cggttgtggc tcgtcgacaa tttcagccgg aactacttca gcggccactt
     2401 ccgcaactgc ggtttcagct tctgcgacag gttccggtgc atactcgcgc tcaggagcgt
     2461 aactactgac tgctggttcg ggcgtcgctt cctgatgttc ggtttgcgga gcgggcgctt
     2521 tcgctggagc ttgtgcggct aacagatcga cacacatccc gcacatctgc accaatgttt
     2581 ccaacgcttc gaggttgatt gcttctgaat caagagaacc agaatcggcg tagaggacag
     2641 ccactgcgcg cttgcgcacg ttcaggggta tgctgacaac tcgttccggc ggcgcctctt
     2701 ggcccaatcg gttaagcagt acgtaattct ctgagtgaga gtgagcaccg ccgctccagg
     2761 tcgtgcgcga cttggcgacg tcagacagta gattatcgcc ggagagcgga atcgaaagct
     2821 gttgtaccgc gctatcgccc acgctacctt caaagccgcg ggcacgccaa ccaattgcgt
     2881 tatcgccttt gacggcgaag aaagcgacgc gcggggcgaa agaggcggct cggttcacta
     2941 gcgttttcag gatctctccc tgcgaagttt gttcgcccat ctcagtcacc gcagccttaa
     3001 cgatggccat gtcactcgag gccttcgcgc gcgcggactc tgatgcggcc agatcgattc
     3061 cctcatcgtg agcggctttc aggtgttcca tgatggaagc cgcgacggag ccatccagtt
     3121 gcacttcact ctggcgatcg aggagttggg tgagagcttc gttgagccgg ctctgcagct
     3181 cggcaatctc ctgcttgact ccgcccaggc gcccttcgat atagctctct atctcatggc
     3241 gcaggctttc ttccagtttt ccactgacca cagttgtttc ctccattgct cgggtcgtag
     3301 gcatgaccca ggttcgtagg cggggctgct aattcgggtc aaacgctttt ccagataggc
     3361 cttgcaggac cgcacggggc ctgaaccgat tatggacgtg cgagattcag cggtcaatag
     3421 tcttcatcag ggggccgaac cggtactgtt tcgggcgcag ttctgaccct gggcgcccgt
     3481 ttctcgcgtt tgacagccgt gtaaccggca gcttataaaa gttcgactcc aacatgcgaa
     3541 ggcagtcgta acccccatgg cagccacaat tctcattgcc gacgattacg aagacaatct
     3601 cgagctcctg cgtctgatgc tggaagccgg atcgtatcgc gtgcgcgaag cgcgcgacgg
     3661 ccgtcagtgt gtgaccatgg cgctggcaga accgcctgac ctggtcatga tcgatctatc
     3721 catgccgttg ttggatggct ggggggttat tgaagaactg cagaacgacg agcgcacggc
     3781 ggggatcccg tgcgtggccg tgactgctta cggcgactca gatcgtgagc gcgcactaca
     3841 aacgggtttt gatgcttata tcgccaagcc ttttcgcagt gcggagttgt tacaggtagt
     3901 cgcaagcctg atcggcacca gcaaaaccaa aagctcgcct cagggtttgg agcgtgccga
     3961 cctgccggcg ctttgatact taagctcatg gctaggaagg ttggcttgcc accgcttctt
     4021 tttttccaat caagttgtcg gcgaaaca
//
LOCUS       DASUEX010000181         6113 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1403929, whole genome shotgun sequence.
ACCESSION   DASUEX010000181 DASUEX010000000
VERSION     DASUEX010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6113
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1403929"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            8..6022
                     /locus_tag="VFV61_03015"
     CDS             8..6022
                     /locus_tag="VFV61_03015"
                     /inference="COORDINATES: protein motif:HMM:NF028476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HEX4899473.1"
                     /translation="MLPAEVLVVLAGISVFLVRPAASRVMTPFLVVWAFSPLVAYLVS
                     RRTTRAPRVMAGEDLQFARTVARRTWRFFENFVGTEDNWLPPDNYQEDPKPFVAHRTS
                     PTNIGMLLLSTAAAHDFGYLGLTEFAQRQTSTFATLGKLAKLHGHFFNWYDTKTLQPL
                     TPQYISTVDSGNLAGSLIALKQTCIELPDLKLFDARTLEGLKDTLVAINFEAEHIVAS
                     RQRTAFVTARQLREEIAASQQLLDAPASQSLTFWASLFESLRRHGSTIEDIVDALAQE
                     HGTETYEELRWWAGRFSHQVSSFESDLRELTPWAFGSFAKNLKPPAADMAPEKPEGWP
                     AIVASLNAVPTLADLPELVDSALVQLAALGNSEPTDSTNADNLLEEPAIVLTSALENA
                     AAAARELLSRLSSLAHECEQTFDEMDFHFLFDEERKLFAIGYNVSDLRADNSFYDLLG
                     SEARLASFVAIAKGDVPQEHWFRMGRQLTSVNGSQALISWTGTMFEYLMPLLVMRTYE
                     GTLLDQTYRTVVARQIEYGRERGVPWGISESAYNARDLQLNYQYGPFGVPGLGLKRGL
                     IEDLVTSPYATVLAAMIHPRAAMENLRALARDGALSAFGFYEAIDYTSERLPQNQKRV
                     LIRAWMTHHQGMSLVALNNALLNGRMEQRFHSDPLVQATELLLQERIPVGVVAAHPRA
                     EEVLSGRVIRSLPGMITRVYNSANLETPRTQLLSNGTYHLMITTAGSGYAACGRNAVT
                     RWREDVTRDNWGAFVYLRDVRSGSVWSAGHQPVQRRAQSYEVSFSEDKADFWRTDAGM
                     ITHMEVVVSAEDNAEVRRISLTNNSLRTREIELTSYAEIVLAPQQADAAHPAFSNLFI
                     ETEFFAAENALLAHRRQRAVEDEKIWAVHAVVAEGDTLGAVQYETDRGRFLGRGHTPA
                     DPIAVMEERPLSNTVGAVLDPVFSLRRRVRIKPNQTARVSFSTAIASSREQAMMLADK
                     YHDPNIFERESRLAWTKAQVEMSHLSLDAEDAHLFQRLAARVLYSDPTLRPRPHVLAL
                     NTKAQSSLWLYAISGDLPILLVRVNRLEDLQMVRKILRGHEYLHYKGLKIDLVILNDH
                     PPGYMQILHKELELLLRTTGLQALQDKPGGIFLRRADIMPEADRILLHAVARVVIVTE
                     RGSLEDQLERSAVAELLPPPFVPRLPSQSYPEPTVALPELTFFNGLGGFHQGGREYVI
                     VLGADQWTPAPWANIIANESEFGFQVTETGAGYTWSANSRENRLTPWSNDAVSDPPGE
                     AVYLRDEDTGTVWSTTPAPIREAEPYTIRHGQGYTVFEHTSHGISQELLVFAPLKGSV
                     KISLLRLRNRTDRKRRLTITQYNELVLGFSRSSSAPYIITEIDPESGRIFARNPFNNE
                     FASRVAFVATNDTVSSATCDRKEFLGRNGSPAQPAALRRAHLAGRDGAGLDPCAALQV
                     SIELAPREAREVIFLLGEAESKEAAETLISQFSQPAIVNGAFEEVLSYWDELLGTVEV
                     HTPDAALNTMLNRWLLYQTLSCRVWARSAFYQSGGAFGFRDQLQDVMALVYSRPAIAR
                     EQIIRASGRQFKEGDVQHWWHPPTGRGVRTRFSDDLLWLPFVTSFYITVTGDTGVLDE
                     VVPFLEMPLLGPEEHELYMQPVVSTETATVFEHCARALDRSLAVGPHGLPLMGGGDWN
                     DGMNRVGHLGKGESVWVGWFLHTTLARFAPLCEPRKESSRAERYLKHLENLKLALEEH
                     GWDGDWYRRAYFDDGTPLGSVQNEECRIDSIVQSWGVISGAAENYRSVRAMAAVEEYL
                     IRRGDGLVILFTPPFDRSGLDPGYIKGYVPGVRENGGQYTHAAIWTLIAYAMLGDGER
                     AGELFSLLNPINHASTRAGLHKYKVEPYVAVGDVYAVPPHTGRGGWTWYTGSASWMYR
                     AGLESILGFKLRGERLQIDPCIPQGWREFEIIYRRGKTTYRIKISNPLALHRGIASLE
                     MDGTKLEGNEIGLRDDGLTHAVHVVLGELPAKSENETEVKQQARNN"
BASE COUNT         1279 a         1739 c         1759 g         1336 t
ORIGIN      
        1 gtggttcatg ctgccagccg aagtcctggt ggtactcgct gggatttctg ttttcctcgt
       61 ccgcccggcc gcgtcccgtg tgatgacgcc attcctggtc gtctgggcat tttcacccct
      121 ggtagcttac ctcgtcagtc gccgaaccac tcgagcaccg cgggtgatgg caggggaaga
      181 tctgcaattc gcgcgaacgg tggcgcgtcg cacctggcgt ttcttcgaga atttcgtcgg
      241 cacagaagac aactggttgc cgcccgacaa ctatcaggaa gatcccaagc cgtttgtcgc
      301 gcatcggacg tccccaacga acattggcat gcttctgctt tccactgcag cagcgcatga
      361 tttcggttat ttggggttga ccgagtttgc gcagcggcaa acgtcgacgt tcgcgacctt
      421 aggaaagctg gctaagttgc atggccactt cttcaattgg tacgacacca agacgctgca
      481 acccctgaca ccgcaataca tttccacagt tgatagcggc aatctcgccg gcagcctgat
      541 tgccttgaag cagacgtgta ttgagcttcc ggatctgaaa ctgtttgacg ccagaactct
      601 ggaaggcttg aaggacacgc tggtggcaat taatttcgag gcggaacaca ttgtcgcgag
      661 tcgtcagcga actgccttcg ttactgcgcg ccaactgcgg gaggagattg cagcaagcca
      721 acagctactt gacgcgcctg cctcgcaatc gctgaccttt tgggcctccc tcttcgagtc
      781 gctgaggcgt catgggtcaa ccatagagga catcgttgat gcgctcgcac aggaacatgg
      841 aaccgaaacg tatgaagagc tgcgctggtg ggcgggaagg ttctcgcatc aggtcagctc
      901 gtttgaaagt gacctgcgcg agctgacacc ctgggctttt ggctcgttcg cgaagaacct
      961 gaagccgcca gcggcagaca tggcgccgga aaagccggaa ggctggccgg caatcgttgc
     1021 gtccctgaat gctgttccta cgctcgccga tcttcccgaa ctcgtcgata gcgcgctggt
     1081 gcaactcgcg gcgctgggca actcagaacc aacagactcg accaacgctg acaacttgct
     1141 tgaagagcca gcgatcgttt taaccagcgc gctcgaaaac gctgcggccg ctgctcgcga
     1201 actgctctcg cggctgtcgt cgttagccca cgagtgtgaa cagaccttcg atgagatgga
     1261 tttccacttt ctctttgacg aggaacgcaa gctgtttgca atcggctaca acgtctctga
     1321 cttgcgagct gacaactcat tctacgatct gcttggttcg gaagcgcgtc tcgcgagttt
     1381 cgtagcaatc gccaaaggtg atgtccctca ggaacattgg tttcgaatgg gacggcagtt
     1441 gacttctgtg aacggcagcc aggcactcat ctcctggacg ggcacaatgt tcgaatacct
     1501 gatgccgtta ctggtgatgc gcacctacga aggcacgctg ctcgatcaaa cttaccgcac
     1561 ggtggtggcc cgtcaaattg agtatggcag ggagcggggc gtgccctggg gcatttctga
     1621 gtcggcctat aacgcgcgcg atctgcaact gaattatcaa tatggtccgt ttggtgtacc
     1681 gggcctgggt ttgaagcggg gattaatcga agacctcgta acgtcccctt acgcaaccgt
     1741 gctcgccgca atgattcatc cccgtgcggc catggagaac ctgcgggcgc tcgccaggga
     1801 tggcgcgctc agtgcttttg gcttttatga agctattgat tacacgagcg agcggctgcc
     1861 acagaaccaa aagcgggtgc tgattcgcgc ctggatgacg caccatcagg gaatgagcct
     1921 ggtagctctg aataacgcgc ttctaaatgg gcgtatggag cagcgctttc acagcgaccc
     1981 gctggtccag gcaactgaac tgttgcttca ggaaaggatt cccgtcggtg tggtagctgc
     2041 acatccgcgg gcagaggagg ttctgtcagg gcgcgtgatt cgatccttgc ccggcatgat
     2101 cacgcgcgtc tacaactccg caaacctgga gacgccacgc acgcaactgc tctccaacgg
     2161 aacctatcac ctgatgatta cgacggccgg atccggctac gccgcctgcg gtcgcaacgc
     2221 ggtcacgcgc tggcgggaag acgtcacgcg cgacaactgg ggcgcatttg tttacctgcg
     2281 cgacgtgcgt agcggcagtg tttggtcggc cggtcatcaa ccggtgcaac gccgtgccca
     2341 gtcgtatgaa gtttcctttt ccgaggacaa ggccgatttc tggcgcacgg acgccggcat
     2401 gatcacgcac atggaagtcg tagtttccgc ggaagataac gctgaagtgc gtcgcatttc
     2461 gttaaccaat aactcactgc gtactcggga aatagaactg accagttacg ctgagattgt
     2521 tttggcgccc caacaggcag acgccgccca cccagcgttc agcaatctat tcatcgagac
     2581 tgagttcttt gcggccgaga acgcgttact ggcacaccgg cgccagcgcg ccgtcgaaga
     2641 cgaaaaaatc tgggcggttc acgctgtggt cgcagaaggc gatacgcttg gcgccgttca
     2701 atatgaaacg gatcgcggac gttttcttgg tcgcggtcat acgcctgctg atccgatcgc
     2761 cgtgatggaa gagcggccct tatcaaatac tgtgggcgca gttctcgatc cggttttcag
     2821 tctgcgccgt cgcgtgcgca taaaaccaaa tcaaactgcg cgtgtctcat tcagcacggc
     2881 catcgccagc agtcgtgaac aggcgatgat gctggccgat aaatatcacg acccaaatat
     2941 cttcgagcgc gagtcgcgat tggcctggac gaaagctcag gttgagatga gccacttgag
     3001 cctggatgcc gaggacgctc atctcttcca acgtctcgcc gcccgggtgc tctattcgga
     3061 tccgacactg cgtccgcgtc cgcacgtatt agcactcaac accaaagctc agtcgagcct
     3121 gtggctttat gcgatcagcg gggacttgcc gatcctgctg gtgcgcgtca accgcctcga
     3181 agatctgcag atggtgcgaa agatccttcg cggtcacgaa tacctgcatt acaagggtct
     3241 gaagattgac ctcgtaatcc tcaacgatca cccgcccggt tacatgcaga ttctgcacaa
     3301 agaattggag ttgctcttac gcaccacagg tctgcaagca cttcaggata aacccggcgg
     3361 catcttcctc aggcgcgcgg acataatgcc ggaagcggat cgcattctgc tgcacgcggt
     3421 ggcgcgagtt gtgatcgtca ccgagcgcgg ttcgcttgaa gatcagctcg aacggtctgc
     3481 ggttgcggag ttgttgccac ccccattcgt gccgcgcttg ccctcccaga gttatcccga
     3541 accgacggtg gccttgccgg agctaacctt cttcaacggc ctcgggggat ttcatcaagg
     3601 cgggcgcgaa tacgtgatcg tccttggcgc agaccagtgg acgccggctc cctgggccaa
     3661 catcattgcc aacgaaagcg agttcggctt tcaagtaacg gagaccggcg ccggttacac
     3721 ctggtcagca aacagccgtg agaatcggct cacaccatgg tcaaacgacg ccgttagcga
     3781 tccccccggt gaggccgtct acttgcgtga tgaagatacg ggcaccgttt ggtcaaccac
     3841 ccccgctccc atccgcgaag ctgagcccta taccatccgc cacggtcagg gctataccgt
     3901 gttcgagcac acgagccacg gaatctccca ggagttgtta gtctttgcgc cgcttaaagg
     3961 gtcggtgaag atttcactgc tgcgcttacg taaccgtacg gaccggaagc gtcgcctaac
     4021 gattacgcaa tacaacgaac tggtgctggg ctttagtcgg agcagttcgg ctccttacat
     4081 catcaccgaa attgatcctg agagcgggcg aatcttcgcg cgcaatcctt tcaataatga
     4141 gtttgccagc cgcgtggctt ttgttgccac caacgataca gtttcgagcg cgacctgtga
     4201 tcgtaaagaa tttctcggtc gcaatggctc cccggcgcaa cccgcagccc tgcgtcgcgc
     4261 tcacctggca ggccgcgatg gcgccggcct tgatccctgc gccgccttac aagtctccat
     4321 tgaactcgcg ccccgcgaag cgcgtgaagt tattttcttg ttaggcgaag ccgaatcgaa
     4381 agaggcggcg gagacattga tttcacagtt cagccagccc gcgatcgtaa atggagcctt
     4441 tgaggaagtg ctttcttact gggacgaatt gttgggaacc gtggaagtgc acacgcctga
     4501 tgcagcctta aacacaatgc tgaatcgctg gctgctttat cagaccttgt cctgtcgtgt
     4561 ttgggcgcgc tccgctttct atcagtccgg cggcgccttt ggttttcgcg atcagttgca
     4621 ggacgtaatg gcgcttgtgt acagccgccc tgcaatcgct cgcgaacaaa tcattcgcgc
     4681 ttccggtcgt caattcaaag aaggcgatgt gcagcattgg tggcatccgc caactggacg
     4741 tggggtacgc acacgcttct cggacgatct cctctggctg ccgtttgtga cttcgttcta
     4801 cataacggtc acgggcgata ccggcgtgct tgatgaagtg gttccctttc tggaaatgcc
     4861 gctgttggga cctgaagagc acgagcttta tatgcaacca gtagtctcga ccgagacagc
     4921 aaccgttttc gagcactgtg cgcgggcgct ggatcgaagt ctcgccgtcg gcccgcatgg
     4981 attgccatta atgggcggcg gcgattggaa cgacggcatg aatcgcgttg gtcatctggg
     5041 taagggcgaa agcgtctggg ttggctggtt ccttcatacg actctcgcgc gcttcgcgcc
     5101 cttatgcgaa ccacgaaagg agtcgtccag ggccgagcgc tatctcaaac atttggagaa
     5161 cttgaagctc gctcttgagg aacatggttg ggacggcgac tggtaccggc gggcctattt
     5221 cgacgatgga acgccgctcg gttctgttca gaatgaggag tgccgtatcg actccatcgt
     5281 ccagtcctgg ggcgtcattt ccggagccgc agaaaactat cgatcggttc gcgcgatggc
     5341 cgccgtcgag gaatacttga tccggcgggg cgatggtctc gtgatcctct tcacgccgcc
     5401 cttcgataga agcggtttgg atccgggata catcaagggc tatgtacctg gtgtgcgcga
     5461 gaacggagga caatacactc acgccgcaat ctggaccctg atagcctacg cgatgctagg
     5521 cgacggcgaa cgggcgggcg aactgttctc cctcctgaac cccatcaatc atgcgtcgac
     5581 acgtgccggc ctgcacaagt ataaagtcga accgtatgtt gccgtaggtg acgtttacgc
     5641 tgtgccaccg catacaggac gtggcggttg gacctggtac accggttcgg cgagctggat
     5701 gtatcgtgcc ggacttgagt cgatcctggg cttcaaatta cgcggcgagc gacttcagat
     5761 cgatccctgc ataccacagg gctggcgcga gtttgaaatc atctatcggc gcggcaaaac
     5821 aacttatcga atcaagataa gcaacccgct ggcgcttcat cgtggtatcg ccagtctgga
     5881 gatggacggc accaaactgg aaggcaacga gatcggtttg cgtgacgacg gtctaaccca
     5941 tgcagtgcac gtggtgctcg gcgagctacc agcaaagagc gagaatgaga cggaagtaaa
     6001 acagcaggcg cgtaacaact aatctgttct tgagtggagt tcgttcaagt atgccggtaa
     6061 ttgatctgat cagaggccaa taatcttctt gtccagcagg gtcttcatca tgc
//
LOCUS       DASUEX010000182         5594 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_251313, whole genome shotgun sequence.
ACCESSION   DASUEX010000182 DASUEX010000000
VERSION     DASUEX010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5594
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251313"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..524
                     /locus_tag="VFV61_03020"
     CDS             <1..524
                     /locus_tag="VFV61_03020"
                     /inference="COORDINATES: protein motif:HMM:NF014872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899474.1"
                     /translation="VARAGRVVEVPGDTVQWNYRHTSFKDGELLLGATLRLTPDDAAA
                     IMARMEDAKSRRLATQPHGGRSAGCFFKNPPASSIGTGKMIDEMGMKGVRRGTALVSP
                     VHANFIVTEGENARAEDALALAEEIRERVKREQGIELEYEVELWRANEPRKDESSSSA
                     PAVSPDAVEKEST"
     gene            521..1609
                     /locus_tag="VFV61_03025"
     CDS             521..1609
                     /locus_tag="VFV61_03025"
                     /inference="COORDINATES: protein motif:HMM:NF020067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HEX4899475.1"
                     /translation="MREQVIPQKVGNRSGLNPKKRSGPVAQRPTRRDAEGRSFGMRVR
                     VLIGYVPVLLKVIIAIAVGVGVFAAYRAASAASFFQLRSVEVQGESRASLQDVQSLVK
                     REVTKSGVWQADLSELSAKLERLPWVRRAVVSRVLPDGIRVRLVEREPRAVVRTAAGR
                     FIWVDEDAVMLGEMLPADQLPPFFLRGWNEEEGEAERKENSERVQKFMELRRDWEALG
                     LAERVSEVNLIDLRDVRAQLAADDSQIEVRLGSQDLGKRLRQALEVLDGQRNTPRGSA
                     ISYIDLNQGKGAIVGFVSGAHAFSAQPGTKPNATANTAAPERRRSESDREQRLANRSA
                     RDKKKDAETKSTRKETNADARPRRVRST"
     gene            1655..2917
                     /gene="ftsA"
                     /locus_tag="VFV61_03030"
     CDS             1655..2917
                     /gene="ftsA"
                     /locus_tag="VFV61_03030"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEX4899476.1"
                     /translation="MAKRTPHTIGLDIGTSRVTCIACEPGDGGLLNIVGIGEAEARGL
                     RKGVIVNPDAAVDAIKKAVEEVERMSDVQAEEVTINLAGSHILGLNGQAIVAVSGRDR
                     EITPEDVRRAIDSACAIQLPAGREIVDRLPQEFIVDDQDGINDPVGMIGARLAVKVHI
                     VTSPVTARQNAINAVNRAGLIVADMVLEQLAAAEASLSEDDKEFGSALVDIGAETTGL
                     VIFQRGAVQHTAVFPLGGSHFTNDIAFGLRTPIPEAEKIKRTVGSACSTSLSEMERSE
                     LVEVPSVGGRAPRQLSRQILCDILQPRAEEVLMHVADEIKDSGWERQLSSGVVLTGGG
                     ALLSGMTEIAEQVFDAPVRLGFPERDRFGGLVEDIQSPAWAAAAGLSLLASRAQASEM
                     RAGMSRRGSTGKLTHFVSKFRNRFGSIF"
     gene            3051..4202
                     /gene="ftsZ"
                     /locus_tag="VFV61_03035"
     CDS             3051..4202
                     /gene="ftsZ"
                     /locus_tag="VFV61_03035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEX4899477.1"
                     /translation="MAADARKPGTGINIFIDDDPPITGARIKVIGIGGGGGNAVNRMI
                     EAGIEGIEFLVANTDLQALKRSRAPIKIQLGSRLTKGLGAGADPGVGREAALEDTDKI
                     IEVLEGADMVFVTTGMGGGTGTGAAPIIASLATELNCLTVAVVTKPFQFEGRRRMQQA
                     DHGLRELRECVDTVITIPNERLLHTVEKAASLSDSFRIADDVLRQAVQGISDLITVPG
                     LINLDFADVKAIMSGMGMALMGAGHAFGENRAIEATQQAISSPLLEEASIQGAKGVLI
                     NITGGTDLTLYEVNAAAMIIREAADEDANIIFGAVIDEAMRDQMRITVIATGFDRESV
                     AVAAAGSTSTLPANAPRTIPRPLDDLPRPMMSARADDLDVPTFIRKKAD"
     gene            4497..4907
                     /locus_tag="VFV61_03040"
     CDS             4497..4907
                     /locus_tag="VFV61_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899478.1"
                     /translation="MNRKLIRTTLAEVKGKARETPPPRERSNGGFQRSSGGEGGREPQ
                     QRPAQPSSVQVRKRVPPPMETNAEIFYYKKQIDAHTPMVIVLTDGEEIEGTMEWYDRG
                     AIKVNRLAAPNLLVLKRNIKYMYKADERVEQSAD"
     gene            4980..>5594
                     /locus_tag="VFV61_03045"
     CDS             4980..>5594
                     /locus_tag="VFV61_03045"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:NF016084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEX4899479.1"
                     /translation="MGQFANSSSAFGQRRRSLPRVAITMGDPAGIGPEVVLKAVAEDE
                     IRAVCVPFIIGDAQLLAHTARTLDLQCGYDIIRQGEEIPDEVAGPVIFHLDNIGGYIE
                     PGIESGAAGKAAAGYIEAAVDLCAAGSVDAIATAPINKRALFLGGYSFPGHTEFLAHL
                     TNTEEFAMAFVAANLRIVLLSTHVPLAEAIRMVTRERIIKTIHLA"
BASE COUNT         1258 a         1446 c         1710 g         1180 t
ORIGIN      
        1 gcgtcgctcg cgccggacgg gtcgtggagg ttccgggcga cacagtgcaa tggaattatc
       61 gtcacacctc tttcaaggac ggcgagctgc tgttgggagc gaccttgcgg ctgactccag
      121 acgatgccgc agcgatcatg gcgcgcatgg aagacgcgaa gagccgccgg ctcgcgactc
      181 aaccacacgg cggtcgttca gcgggttgtt tctttaagaa tccgccagcc agctctatcg
      241 ggaccggcaa gatgattgat gagatgggta tgaaaggagt gcggcggggg acagcgctgg
      301 tttctcccgt acatgcgaac ttcattgtga cggagggtga gaacgcgcgg gctgaagacg
      361 ctttagccct ggcggaggag attcgcgagc gcgtgaagcg tgagcaggga attgaactcg
      421 agtatgaagt tgaactctgg cgcgcgaacg aaccgcgaaa ggacgaatcg agtagcagcg
      481 caccagccgt ttcccctgat gcggttgaga aggagtcgac gtgagagaac aggtcatccc
      541 ccagaaagtc ggaaatcgta gcggccttaa ccctaagaaa cggagcggcc cagtcgccca
      601 acggccgact cgtcgtgacg ccgaaggtcg atcatttggc atgcgagtgc gtgtcctcat
      661 cggctacgtt ccagttttgt tgaaagtgat catcgccatt gccgtaggtg tcggagtctt
      721 cgccgcatac cgggcagcgt ccgcggcaag tttttttcag ttgcgtagcg tcgaagtaca
      781 gggcgagtcg cgcgcctcgc tccaggatgt ccagagtttg gtgaaacggg aggttacgaa
      841 gtcaggagtg tggcaggctg acttgagtga gctaagcgcg aaacttgaac gcctgccgtg
      901 ggtcaggcgc gcagtcgttt cccgtgtatt gccggatggt attcgggtcc ggcttgtgga
      961 acgagagccg cgcgctgtgg tgcgcactgc agccggccgc ttcatctggg tagatgaaga
     1021 tgcggtaatg ctcggcgaaa tgctgcctgc cgatcaactg ccgcctttct ttttgcgtgg
     1081 ctggaatgaa gaagaaggtg aagcggagcg gaaagaaaac agcgagcgtg tgcaaaagtt
     1141 tatggagctg cggcgcgatt gggaggcgct gggtttagcc gagcgcgtca gcgaagtgaa
     1201 tctaatcgat ctacgcgacg tacgggcgca attggcggca gacgattcgc aaattgaagt
     1261 gcgtctgggt tcacaggatc tcggcaagcg attgcggcag gcgcttgagg ttttggatgg
     1321 acagcgaaat acgccgcggg gatcagcgat cagctatatc gatctcaacc agggtaaagg
     1381 cgctatcgtt ggatttgtgt cgggggcgca tgccttcagc gctcagcctg gtaccaaacc
     1441 taacgcgacg gctaatactg cggcgccgga aagacgacgg tccgaatcag atcgcgaaca
     1501 aagacttgcg aacaggtctg caagagacaa aaagaaagac gcggaaacaa agtctacgag
     1561 gaaagaaact aacgccgatg cgagaccacg acgagttcgg tccacctagc tcgaacctga
     1621 gaacccgaat ggttttgtta aggagcaaaa gttgatggcc aagcgcaccc cccacacgat
     1681 cggcctggat attggaacca gcagggtgac gtgtatcgct tgcgagcccg gcgacggcgg
     1741 gctgctgaac atcgtgggca ttggcgaggc ggaagcccgc ggcctgcgca agggtgtgat
     1801 tgtgaatccc gacgccgcgg tcgatgccat caagaaggcg gttgaagaag tcgagcggat
     1861 gagcgacgtg caggctgagg aagtgacgat caacctggcc ggttcgcaca tcctcgggct
     1921 taacgggcaa gccatcgttg ctgtctcggg acgcgatcga gaaataactc cggaagatgt
     1981 gcgtcgcgct atcgattccg cctgcgcgat tcaattgcca gccgggcgcg agatcgtgga
     2041 tcgactgcca caggaattta tcgtcgacga tcaggacgga atcaacgatc cagtgggaat
     2101 gatcggagcg cgtctcgcag tcaaagtgca cattgttacc agtccggtta ccgcgcggca
     2161 gaacgccatt aatgcagtta accgggcggg cctgatcgtt gccgatatgg tgctggaaca
     2221 gctcgctgcc gcggaagcgt cactctccga ggacgacaag gaatttggct cagctttggt
     2281 cgatattggc gcagaaacga cgggtttggt gatctttcag cggggcgccg tccagcacac
     2341 ggcggtcttt ccgctgggag ggtcgcactt cactaacgac atcgcctttg gtttgcgtac
     2401 gccgattccg gaagcggaaa agattaagcg aacagtgggg agcgcttgca gtacctcact
     2461 gagtgaaatg gaacggagcg agttggttga agttccctca gtcggcggcc gcgctccgcg
     2521 gcaactctca cggcagattc tttgcgacat cctgcaacct cgcgcggaag aagtgctgat
     2581 gcacgtcgcc gatgagatta aggattctgg ttgggagcgt cagctttcaa gtggcgttgt
     2641 gctcaccgga ggcggtgctc tcttaagcgg catgaccgag atcgcagaac aagtgtttga
     2701 cgcgccggtg cgccttggtt tccctgagcg tgatcggttt ggtggtttgg ttgaagacat
     2761 tcagagtcct gcctgggccg cagcggcagg attgtcgtta ctcgcatcgc gcgcgcaggc
     2821 gagcgaaatg cgcgccggca tgagccggcg tggttccacc ggaaagttga cacacttcgt
     2881 gtcaaagttt cgaaatcgtt ttggtagtat tttttgacaa gttgttgtat gctctcgccc
     2941 cggttgcaca agatattgtc cgcaatatat ggacgccgat ggcacgagct acccaatata
     3001 gaagaaaagg gccttggaag ccttcccgct ttgggagaga ggaaatgact atggcagcgg
     3061 acgctcgtaa acccggaact ggcattaata ttttcattga tgatgatccg cccatcacag
     3121 gggcacgcat aaaggtaatc ggaattgggg gcggcggcgg taatgcggtc aatcgcatga
     3181 ttgaagccgg cattgagggc attgagtttc tcgttgccaa tactgacttg caggcgctca
     3241 aacgttcgcg cgcgcccatc aagattcagc ttggcagccg tctcaccaaa ggtctaggtg
     3301 ccggagccga tccgggcgtg ggtcgcgaag cagcattgga agataccgac aagatcatcg
     3361 aagtgctgga aggcgcggac atggtgtttg taacgaccgg tatgggtggg ggcacgggca
     3421 ccggcgcggc tccaatcatt gcctcgctgg ccaccgagtt gaactgtctc acggttgccg
     3481 tagtaacaaa gccgtttcag tttgaaggtc gacgacgcat gcagcaggca gatcacggtc
     3541 tgcgtgagtt gcgcgagtgt gttgacacgg taatcacaat tccgaatgag cggctgctgc
     3601 atactgtgga aaaggccgct tcattatccg actcgtttcg cattgccgat gatgtcctgc
     3661 gccaggcggt ccagggcatc agcgatctga tcacggtacc cggattgatc aatctggatt
     3721 ttgctgatgt gaaagcgatc atgtccggca tgggaatggc cctgatgggc gcgggacatg
     3781 cgtttggtga aaaccgcgcc attgaggcaa cgcaacaggc gatctcctca ccgctactag
     3841 aggaagcgtc gatccagggc gccaaaggcg tgctgatcaa tattaccggc ggcaccgatc
     3901 tgacgcttta tgaagtgaat gccgcggcga tgatcattcg cgaagccgcc gatgaagatg
     3961 ccaacatcat ttttggtgca gtgattgacg aggcgatgcg cgaccagatg aggatcacag
     4021 tgatcgctac aggctttgat cgggagtctg tggcagtcgc tgcggctggt tcgacctcta
     4081 cgttgcccgc aaacgccccg cgcaccattc ccaggccgtt agacgacctg ccgcgcccga
     4141 tgatgagcgc tcgcgcggac gacctcgacg tgcccacttt cattcgcaaa aaagccgact
     4201 aagaatcgga gcgaggttag cttgaagcag ggaaggctga aaagccttcc ctgctttttc
     4261 gcaagctcac gcctgcaggt ttcttatgtt ttcactttgc tttttttttt ctggttgtgt
     4321 agcatgtgcc cattccctgg caggtttttc cattacgagc aaaaatatcc aatctcctcg
     4381 agtggccgca gcctgcgaga gttttgtttt atcgatctcc gtcagctctt cccttcacat
     4441 gatgtgcctg gcacgatcga tccaaacccg tcctattttt tggaggtaat catcaagtga
     4501 atcgtaaact tataagaact acccttgcag aggttaaagg taaagcgcgc gaaactccgc
     4561 caccacgaga gagatccaac ggtggctttc agcgtagctc tggcggcgaa ggtggaaggg
     4621 aaccgcaaca gcgtccggca caaccatcat cggtgcaagt tcgcaaacgt gtgccaccac
     4681 cgatggaaac aaatgcggag atcttttatt acaagaagca aatcgatgct cacacaccaa
     4741 tggtgatcgt gctcaccgac ggtgaagaaa ttgaaggaac aatggagtgg tatgatcgcg
     4801 gtgcgatcaa ggtcaatcgc ctggcagcgc ccaaccttct ggttctcaaa agaaacatca
     4861 aatacatgta caaagccgac gaacgcgtag agcagtcagc cgactaaacc gccgcagccc
     4921 gtttgtcaga tggacctccc ccaccttcgc cagacgcgaa ttatgcgtcg tctcaactaa
     4981 tgggacagtt cgctaatagc tcaagcgcct ttggtcagcg ccggcgatcg ttaccgaggg
     5041 tcgccattac catgggcgat ccggcgggta tcggtcctga ggtcgttctc aaggccgtcg
     5101 ccgaggatga aataagggca gtgtgtgttc cctttatcat tggcgacgcc cagttgttgg
     5161 cccacaccgc ccgcactctg gacctgcaat gcggctacga catcattcgt caaggtgaag
     5221 agatacccga tgaagttgcc ggcccagtaa tctttcatct ggacaacatc ggtggttaca
     5281 tcgaacccgg cattgaatcg ggtgccgctg gcaaggcggc ggcgggatac atcgaagcag
     5341 ccgttgatct ttgtgcggcg gggagtgtcg atgccatcgc caccgctccc atcaacaaac
     5401 gcgcgttgtt tctggggggc tacagttttc ccggccatac agaattcctg gcacacctga
     5461 cgaatactga agaatttgca atggcgtttg tggcagcaaa cctgcgcatc gttttgcttt
     5521 ccactcatgt tcccctggct gaggcgattc gaatggtgac gcgcgagcga atcatcaaaa
     5581 cgattcacct ggca
//
LOCUS       DASUEX010000183         2136 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1525703, whole genome shotgun sequence.
ACCESSION   DASUEX010000183 DASUEX010000000
VERSION     DASUEX010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1525703"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /locus_tag="VFV61_03050"
     CDS             complement(<1..569)
                     /locus_tag="VFV61_03050"
                     /inference="COORDINATES: protein motif:HMM:NF012670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899480.1"
                     /translation="MPQHSDTTPELDQLCINTIRTLSLDAVQKAESGHPGLPLGAAPM
                     AYVLWTRFLRHNPQNPKWENRDRFLLSAGHGSMLIYSLLHLTGYDLPLAEIENFRQWG
                     SKTPGHPENTLTPGVEITTGPLGQGFANGVGMAMASAHLAAKFNQQDFPVIDHYIYAI
                     VSDGDLMEGVASEAASLAGHLKLGKLIYF"
     gene            complement(618..>2136)
                     /locus_tag="VFV61_03055"
     CDS             complement(618..>2136)
                     /locus_tag="VFV61_03055"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HEX4899481.1"
                     /translation="GFEPSPQLVSVMTGSRPAVVLSLDIGTSGIRAALFDERGEEIEG
                     ANLRLRWPAMHDDATAFNAELRLSWIGKAIDDLLELSIPVAPEIQLISISCFWHSLVG
                     VAEDGDSTTPVFTWASTEATRAAEELRGSFNEPALHARTGCRFHPSYWPAKLRWLSQE
                     ERHRVAATRYWMSFGEFLVLHLCNETAASISMASGTGLFNQQRCDWDAELIDALHIPI
                     EALPKLAGSHTTFQLNAQHAARWPQLSEAKLFPAIGDGAADAIASGCATRNNLALMVG
                     SSGALRLLYRGEPPAQIPPELWCYRADDKRVILGGALSDGGNLYEWLRDSLPNIEEEW
                     IENSLAQIEPDAHGLTILPFWAGERSPGWSLTARGAILGLTSKTQPLEILRAAMEAVA
                     YRFALIHRALVPFAPDAAIVASGTALRRSPVWIKILADVLGTPITLSTHREGSALGAA
                     LLALEAAGKIANIETWNVVAGVTGETFEPDIARHARYQVGLERQQTIYDQLVSET"
BASE COUNT          481 a          583 c          624 g          448 t
ORIGIN      
        1 tagaagtaga ttagtttgcc gagttttaga tgaccggcaa gcgatgccgc ttccgaggcg
       61 acgccctcca tcaagtcgcc atccgagacg atcgcgtaaa tgtagtggtc gataaccggg
      121 aagtcctgct gattaaactt ggctgccaga tgggccgagg ccatcgccat cccgacgccg
      181 ttagcgaagc cttgccccag cggtccggtg gttatttcca caccaggagt cagcgtgttt
      241 tccggatgtc cgggagtctt tgaaccccac tggcggaagt tctcgatttc agcgagcgga
      301 aggtcatatc cggtgagatg gagtagggaa taaatcaaca tcgaaccatg gccagcggaa
      361 agcaaaaagc gatcgcgatt ttcccacttc gggttctgcg gattgtgacg gagaaagcgc
      421 gtccacaaga cgtaggccat cggcgctgcg cccaacggca atcccgggtg ccctgattcc
      481 gccttttgca cggcatcgag ggagagtgtc cgaatcgtgt tgatgcaaag ttgatcaagc
      541 tcaggagtgg tgtctgaatg ctggggcatc ggcgcgctca tttgtgattt tatccttggg
      601 ttgatagttg gtggaggtta agtttcgctg actagttgat cgtagatcgt ttgttgccgc
      661 tcaagcccga cctgatagcg agcatgccgg gcaatgtcgg gttcgaaggt ttctccggtg
      721 actccggcga caacgttcca tgtttcgatg tttgctattt tgcccgccgc ttccaatgca
      781 agcaaggcgg cgccaagtgc agagccttcg cgatgagttg agagcgtgat tggagtaccg
      841 agcacgtctg caagaatctt gatccagaca ggagaacggc gcaaagccgt gcccgaagcg
      901 acgatcgcag cgtcaggcgc aaacggtacc agcgcgcgat gaataagagc gaatcgatag
      961 gcaacagctt ccatcgccgc gcgcagaatt tccagcggct gagtcttgct ggtcaaaccg
     1021 aggattgcac ccctggcagt tagcgaccaa ccgggactgc gttcacccgc ccaaaacggc
     1081 aggatcgtta gaccgtgggc gtcgggttct atctgagcca gagaattctc aatccactcc
     1141 tcttcaatat taggcagaga atcgcgcaac cattcataaa gattcccgcc gtcagaaagc
     1201 gcgccgccga gaattacgcg tttatcatcg gcgcgatagc accagagctc gggaggtatt
     1261 tgcgccggcg gctcgccgcg atacaacagc cgtaatgccc ccgagctgcc gaccatcaag
     1321 gctagattgt tgcgggtagc gcagccgctg gcaatcgcat ctgcggcgcc gtcgccgatc
     1381 gcgggaaaca gtttcgcttc gctcaactgc ggccagcgag ccgcgtgctg ggcgttcaat
     1441 tgaaatgtgg tgtgagaccc ggcgagtttg ggcaaggctt caatgggaat atgaagcgcg
     1501 tcgattagct ccgcatccca gtcgcagcgt tgctggttga acaacccggt tcccgacgcc
     1561 atcgaaatgc tggcagcagt ttcgttgcag agatgtaaaa ccaggaattc tccgaacgac
     1621 atccagtaac gagtggctgc cacgcgatgg cgctcttcct gactaagcca gcgtaatttt
     1681 gccggccagt aactgggatg aaagcgacaa ccggtacgag cgtgaagcgc cggttcgtta
     1741 aatgatcctc tcagttcctc agcagccctc gtcgcttcag tgctggccca ggtgaagacc
     1801 ggagtggttg agtcgccatc ctcggcaaca ccgacgaggc tatgccaaaa gcatgagatc
     1861 gaaatcaatt gaatctcggg agcgacggga attgatagct ccagcaggtc gtcaattgcc
     1921 ttcccaatcc agctcagtct taactcggca ttgaacgcag tggcatcgtc atgcatcgcc
     1981 ggccagcgca gacgaagatt cgcgccttcg atttcttcac cgcgttcatc gaagagtgcg
     2041 gcacgaatgc cggaggtgcc gatgtctaaa ctcaacacca cggcgggacg tgagcctgtc
     2101 atcacagaga cgagttgagg tgatggctca aaccct
//
LOCUS       DASUEX010000184         3295 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_37023, whole genome shotgun sequence.
ACCESSION   DASUEX010000184 DASUEX010000000
VERSION     DASUEX010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3295
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37023"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="VFV61_03060"
     CDS             <1..182
                     /locus_tag="VFV61_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899482.1"
                     /translation="QMRPLQTELKSRLHFDPENSDEEQNQIEIRHKGSGLVGAGDLHV
                     SINGPTAEPYTEKIS"
     gene            142..2286
                     /locus_tag="VFV61_03065"
     CDS             142..2286
                     /locus_tag="VFV61_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899483.1"
                     /translation="MAQQPSPTPKKSPEPQPTVDAGEDAGNYTVIGSIEFGYRGLSVD
                     GDHNKYQSDLNYRTGPRLFDSTFLLKSKDGKGGLFDTLLVTSTGWGGDPNGNVRMSVE
                     KAKWYRFDGTYRRFKYFRFVNSFANPNWVFSPASFSRPPNPVTGEHGTDTRMQLGDFD
                     LTILPKDERIRFNLGYSPERYDGPAFTNYHVGGNEFNLLQQMKSRANDFRVGADGKVG
                     PIDFSFLQGFRRFREDSFIHLGTTPGINLNPAAASLTSFNRDEPARGKVNYTRLSLHS
                     LIAKKLDITGRIVYSKATSDYSLAESFTGRNWNPRVTGWPPTPPAATPNILNLGRYNI
                     TGNTERPNTLGDIGVTLLATDKLRISNTFRVEDFEIDGTAVFSDFFSITRGSGATLRT
                     DTVGFNNLDANRITKYRKYQNTIEGDYQFNSRYSMHIGYRYGKRHIEEIFEGFNLGSN
                     GSVTPPPVRSSDEFEEENHTHAILGGFKARPGNNWTVYFDAEHGTADNVFTRIGNYNY
                     TNIRGKSRFALPSRKVSFTLAVITRNNSNPSEIAGVSLEDFGVDVKSRVFSSTLDWAP
                     TSDLSFSAGYTHHRIDSDAVVEYFFNSVRHFFGSSQYHQKNNFFFVDSVARLHPRITL
                     YAAYRINKDIGQGSLLADPTGNPGTLIASYPMSYQSPEARLAIKLNQRLDWNIGYQYY
                     NYNESALVGPRPQNYHAHLPYTSLRFYFGRRE"
     gene            2422..2904
                     /locus_tag="VFV61_03070"
     CDS             2422..2904
                     /locus_tag="VFV61_03070"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HEX4899484.1"
                     /translation="MANFRGDETNGKTGSAKPQNLTRRSFLDYLLGTSAFATLAAILY
                     PIIRFMAPPQIVEAMQSSVVAAKISELPPNSGKIFKFGSKPGLIVHTASGELKAFSAV
                     CTHLDCIVQYRSDTKQIWCACHNGQYNLNGENIGGPPPRPLEAYPVNKRGDDIVVNRS
                     "
     gene            2911..>3295
                     /locus_tag="VFV61_03075"
     CDS             2911..>3295
                     /locus_tag="VFV61_03075"
                     /inference="COORDINATES: protein motif:HMM:NF012263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HEX4899485.1"
                     /translation="MRIASFGRWLDERLSISTIARTAQKKEVPLHRQSIWYYFGGMTL
                     FLFVVQVVTGILLLLYYRPSAENAFESVQFIMTEVKFGWLIRSIHSWSANLMILTLFI
                     HMFSVYFMRSYNRPRELTWVSGVLLL"
BASE COUNT          815 a          889 c          841 g          750 t
ORIGIN      
        1 ttcagatgag gcctttgcag acggagttga aaagccgttt gcattttgat ccagagaata
       61 gtgatgaaga gcaaaaccaa atcgaaattc gccacaaagg ttctggtctg gttggcgctg
      121 gtgatcttca cgtcagtatc aatggcccaa cagccgagcc ctacaccgaa aaaatctcct
      181 gaacctcaac ctactgtcga cgccggagaa gatgctggca attacacggt catcggcagc
      241 atagagtttg gttatcgcgg tctaagtgtg gacggcgacc ataacaagta ccaaagcgat
      301 ctcaactaca gaacggggcc acggcttttc gacagcactt tcctgttgaa atccaaggac
      361 ggcaagggcg gcctcttcga tactctgctg gtaacttcga cagggtgggg tggagatccc
      421 aacggtaacg tgcgcatgag tgtcgaaaaa gcgaaatggt atcgctttga cgggacctat
      481 cggcgcttca agtattttag atttgtcaac agcttcgcaa acccgaactg ggtattcagt
      541 cccgccagct tcagccgtcc gcccaatccg gtgaccggcg agcacggcac cgatacgcgc
      601 atgcagttgg gagattttga tctaacgatt ctgccgaagg acgagcgaat caggttcaat
      661 ctcggctatt cgcctgagcg ctacgacgga cccgcattca ctaactacca tgtcggtgga
      721 aacgagttta atctgctcca acagatgaaa tcccgtgcga acgactttcg cgttggcgcg
      781 gacggcaagg ttgggcccat tgatttttcg tttctgcagg gttttcgccg cttccgcgaa
      841 gatagcttta ttcaccttgg aacaacgcct gggataaacc tgaatcccgc agcagccagc
      901 ctgacaagct tcaataggga tgaacccgca cgcggaaaag tcaactacac acgactgagc
      961 ctgcattcgc tgatcgcgaa aaagttagac ataactggac ggatcgttta cagcaaggct
     1021 acatccgact attcgttggc agagagcttc acgggcagaa actggaaccc cagggtgacg
     1081 ggatggcctc ccactccccc agcggcaacc ccgaacatac ttaatctcgg ccggtacaac
     1141 attactggaa acacggaacg gccaaacacg ctgggagata ttggcgttac tttgctcgct
     1201 accgataagc tgcggatctc aaacacgttt cgggttgaag acttcgagat cgacgggaca
     1261 gctgtattta gcgacttctt ctcgatcacg cgagggtcag gggctaccct gagaaccgat
     1321 accgtcggct tcaataatct tgatgccaac cgcataacca agtatcgcaa gtatcagaac
     1381 acgattgaag gcgactacca attcaattct cgctattcga tgcacatcgg ttaccgctat
     1441 gggaaacgcc acatcgaaga gatttttgaa ggcttcaatc taggctcgaa tgggtcagtg
     1501 acaccgcctc ctgtccgttc ttccgacgag tttgaggaag agaaccacac tcacgcaatc
     1561 ctcggtggct ttaaggctcg acccggaaac aattggacag tctacttcga cgcggaacac
     1621 ggaacggccg acaatgtgtt cacgcgtatc ggaaattaca actacaccaa tattcgcggg
     1681 aagagcaggt ttgcgcttcc cagccggaag gtgtctttca ccctggccgt aatcacgaga
     1741 aacaactcca atccttccga gatcgccgga gtgtcgttag aggatttcgg cgtcgacgtg
     1801 aagtcgcgtg tgttcagttc cacccttgac tgggctccta cttcggacct ttcatttagc
     1861 gctggttaca cccatcaccg gattgatagt gatgccgtag tggagtactt tttcaacagc
     1921 gtccgacatt tctttggcag tagccagtac caccaaaaga acaatttctt tttcgtggac
     1981 tcggtcgcaa ggctccaccc gaggattacg ctttacgcgg cctatcgaat caataaggac
     2041 atcggacagg gcagcctcct ggcggacccg accggaaacc cgggaacatt gatcgcgtct
     2101 tatccgatga gttaccaatc ccctgaggca cgtctggcta tcaagctgaa tcagcgcctc
     2161 gactggaaca tcggttatca gtactacaac tacaatgaaa gcgcgctggt cgggccgcgg
     2221 ccgcagaatt atcacgcgca ccttccctac acgtcgttgc ggttttattt tggccggaga
     2281 gagtaaggcg ggaaaaagcg cgaaagcaaa acagtttacg ctagcccctg ctcagtctaa
     2341 gtgactgcca gttgggtctt tttgtcgtta gctgacaccc cggctacaac gatcacctgc
     2401 gtccatttcg ggaggatgag gatggcaaac ttccgtgggg atgagaccaa tgggaaaact
     2461 gggtccgcaa aaccccagaa ccttactcgc cgaagctttc ttgattactt gctggggacg
     2521 agtgcgtttg ccaccctcgc cgcgatcctc tatcccataa tcagattcat ggcaccacct
     2581 cagatcgttg aggcgatgca gagttctgtg gtggctgcca aaatcagcga gcttccgcca
     2641 aactccggaa agatcttcaa atttggcagc aaaccagggc tcatcgtcca cactgcatcc
     2701 ggagaactaa aagcattctc ggctgtttgt actcatcttg actgcattgt tcagtaccgc
     2761 tcagacacaa aacagatttg gtgtgcctgt cacaacgggc aatacaacct taacggcgag
     2821 aacataggtg gcccgccccc acgaccactc gaggcatacc cggtaaacaa gcgcggtgac
     2881 gatattgtcg ttaacagatc ttgagagccg atgagaatcg cgagtttcgg acgctggctt
     2941 gacgagcgtc tatcaatcag cacgatcgcc cggacggcgc agaagaaaga agtaccgctt
     3001 catcgccagt ccatctggta ttacttcggc ggcatgacgc tttttctgtt cgtcgtccag
     3061 gtagtaaccg ggatcctgct attgctctac taccgtccca gcgccgagaa cgcttttgag
     3121 agcgttcaat tcatcatgac agaggtcaag tttggctggc tgattcgttc gattcacagt
     3181 tggtcggcaa acctgatgat cctgactctg tttattcaca tgttcagcgt ctatttcatg
     3241 aggtcctaca accggccgcg cgagctaacg tgggttagcg gagtgttgct gctgt
//
LOCUS       DASUEX010000185         4024 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1622759, whole genome shotgun sequence.
ACCESSION   DASUEX010000185 DASUEX010000000
VERSION     DASUEX010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4024
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1622759"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            198..1781
                     /locus_tag="VFV61_03080"
     CDS             198..1781
                     /locus_tag="VFV61_03080"
                     /inference="COORDINATES: protein motif:HMM:NF015237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HEX4899486.1"
                     /translation="MSEDKPAVREKTPTLFRNYISFVGAAITLASLVSVVLLFMLEMT
                     STAHNPYLGIFTYIIFPSILMFGLAVLFLGAILERRRRRRSLEWSVAPYPRLDLNDPH
                     ARRAFFAFLLVTFFFVSASAFGSYRAFEYTESVEFCGASCHTPMKPEFVAYQASPHAR
                     VRCVDCHVGPGAGWYVRSKLSGAYQLYSVSFNKFPRPITTPVHNLRPAPETCEQCHWP
                     EKFFGAQLKVFNHYAYDEKNSNRQVRMLINVGGGSPATGAVTGIHWHMNIGNEVTYVP
                     LDPQRQVIPWVRIKDREGNVTEYHDRRRQLSPEQINLAVKRKMDCVDCHNRPAHVYLP
                     PDVAVDQAFTSGKLDPSLPYLKRKAVESLSGQYDTEPQALSAIEKNLNQFYQTNYADV
                     YSQKQAAIKTAISETQRIFQTYFFPEMKTDWRTHPNNIGHLHFSGCFRCHDGEHVSDS
                     GKTISNDCNVCHTVIFDSARPPAENVKTGSYQHPVDLGSLADRKCDTCHKPNEPFKHP
                     INLGDISLFQCAECHTKKK"
     gene            1838..2296
                     /gene="nrfH"
                     /locus_tag="VFV61_03085"
     CDS             1838..2296
                     /gene="nrfH"
                     /locus_tag="VFV61_03085"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF015237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase small subunit"
                     /protein_id="HEX4899487.1"
                     /translation="MRFRLVPGIVLGITLGLAVGVGAYTFVYAQGYSYMTDNAEACVN
                     CHVMREQYSGWLKSSHRNVAVCNDCHTPPGFVGKYTTKARNGFWHSYYFTTGWFEDNI
                     HITPRGREVTETACRKCHQDIVTAIEGPHPGVADKQTSCIRCHDSVGHMH"
     gene            2301..3779
                     /locus_tag="VFV61_03090"
     CDS             2301..3779
                     /locus_tag="VFV61_03090"
                     /inference="COORDINATES: protein motif:HMM:NF014398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase
                     subunit c552"
                     /protein_id="HEX4899488.1"
                     /translation="MREAAAEERKEIMSEDNEQEKAANGAPAKPARVSRKRRWLRFWG
                     VVALAVVATVFGLALLVNIFQRKQEARDPFFRVVELTDDTVDPAIWGKNFPLQYDDYL
                     KTTDQVRTRYGGSEAEPRTPTQADPRSIVAQSRLEEDPRLKLMWAGYAFSKDFREERG
                     HAYMLEDQMFTERQQVTKQPGACINCHASVYVAYKQLGGEDLYKGFDQLNRMTYAEAR
                     KFVTHPVSCIDCHDSQTMQLRVTRLAFMEGMRVFKASQGIQNYDVNDQASRQEMRSFV
                     CGQCHDEYYFSGPEKRLVYPWAKGLQVENILAFYDEKKFKDWEHADTGAPVLKAQHPE
                     FEMWNQGIHARSGVACADCHMPYKREGALKISDHHVRSPLLNINRACQTCHKWSEDEL
                     KARVETIQGRTHNLRNLAMDALVDLITDIKAARSAGRTDAAVTTAQDFQRRAQFYLDF
                     IESENSNGFHAPQEATRILGESINFSRKGQVALRSSVQAAGQ"
BASE COUNT          941 a         1102 c         1078 g          903 t
ORIGIN      
        1 tgtctgaaac gctctaccgg gaagaacacg ctcttgcata cgaggagaac aaggagaaag
       61 agccaagcga gacctccgca gctgtcggcg gaccagagcc ctccccgcct gaaactccag
      121 ctcagtaact cagcgcgctt gccagatcga gttaatctat atcagtctcg ttgtctgttc
      181 cgcgaacacg gaaaactatg agtgaagaca aaccagcagt tagagagaaa acgcccacac
      241 tatttcgcaa ctacattagt ttcgtgggtg ctgcgattac gctggccagc ctggtcagtg
      301 tggttcttct gtttatgctg gaaatgacga gtaccgctca taatccttac ctgggcatct
      361 tcacttacat catctttccc tcgattctga tgttcggatt agcggtgttg ttcctgggtg
      421 caattctcga gcgccgccgt cgtcgtcgtt ctttggagtg gtccgtggcg ccttacccgc
      481 gactcgatct gaacgatcca cacgcgcggc gcgccttctt cgcatttctg ctggttactt
      541 tcttttttgt ttctgccagt gcgttcggca gttatcgtgc ttttgaatac accgagtcgg
      601 tcgagttttg cggtgcttcg tgtcataccc cgatgaagcc ggagtttgtc gcatatcagg
      661 cttccccgca cgcgcgcgta cgctgcgtgg attgtcacgt gggtcctggc gctggctggt
      721 acgtgcgttc aaaactgtcg ggcgcctacc agctctactc agtttccttc aacaagtttc
      781 cgcgtccaat taccacgccg gtccacaact tgcgaccggc accggaaact tgtgagcaat
      841 gtcattggcc cgagaaattc tttggcgcgc agctcaaggt tttcaatcac tacgcttacg
      901 acgagaagaa ctccaaccgg caagtgcgca tgctcatcaa tgttgggggc ggtagcccgg
      961 caactggcgc cgtcaccggc atccactggc atatgaacat tgggaacgag gttacttacg
     1021 ttccattaga tccgcagcgc caggtaattc cctgggtgcg aatcaaggat cgggaaggta
     1081 acgtgacgga ataccacgat cggcggcggc aactctcacc cgaacagatt aacctggcgg
     1141 tgaagcggaa aatggattgt gtcgattgcc ataatcgtcc cgctcacgtc tacctgccgc
     1201 cagatgtcgc cgtggaccag gcctttacca gcggcaagtt ggatccttcg ctaccttact
     1261 taaagcggaa agctgtcgaa tctctcagtg ggcagtacga cacagaacca caggctctaa
     1321 gcgcgattga gaagaacctg aatcagtttt atcaaacaaa ctacgccgac gtttattcgc
     1381 agaagcaggc cgcgatcaaa accgcgattt cggaaaccca gcgcattttt cagacttact
     1441 tttttccgga aatgaagact gactggagaa cccacccaaa caatatcggg catcttcatt
     1501 tctcgggatg cttccgctgc cacgatggtg aacacgtcag cgactcaggc aaaacgattt
     1561 caaacgactg caacgtctgc cataccgtga tcttcgactc cgcgcggccg cccgctgaga
     1621 atgttaagac cggctcctat cagcacccgg tcgatcttgg cagcctggct gatcggaaat
     1681 gcgatacctg ccacaaaccc aacgaaccct tcaaacatcc gataaacctc ggtgacattt
     1741 ccctgttcca atgcgcagag tgtcatacga aaaagaagta aattccttga ttagttacta
     1801 atcggcgttt gctctgctac gctgggcgac gaaggtgatg aggtttcgtc tggtccccgg
     1861 aattgttttg ggcattaccc tcggcctggc cgtaggtgtg ggtgcgtata cgttcgttta
     1921 cgcgcaaggt tactcctaca tgacagacaa tgccgaagcc tgcgtcaact gtcatgttat
     1981 gcgcgagcaa tacagcggct ggttaaagtc cagccatcga aacgtagccg tttgcaacga
     2041 ttgccacacg ccgcccggtt tcgtcggtaa atacaccacc aaagcgcgga atggcttttg
     2101 gcattcctat tacttcacga ctggttggtt cgaagacaac atccacatca cgccacgcgg
     2161 ccgcgaggtg accgaaacgg cttgccgaaa gtgtcatcag gacatcgtga ctgcgattga
     2221 aggtccgcac ccgggagtcg ccgacaaaca aaccagctgc atccggtgtc atgactccgt
     2281 gggacacatg cactaagaga atgcgggaag ccgcggcaga agaaaggaaa gagatcatga
     2341 gcgaagacaa tgagcaggag aaggcagcaa atggcgcccc agctaagcct gcaagagtct
     2401 ctcgtaaaag acggtggctg cgcttctggg gcgtagtcgc tctcgctgtt gttgcgactg
     2461 tcttcggtct cgcactcctg gttaatattt ttcagcgaaa acaggaagcg cgcgatcctt
     2521 tcttccgagt cgtggagttg acggacgata cagttgaccc ggcgatctgg ggcaaaaact
     2581 ttcccttgca gtatgacgac tatttaaaaa caaccgatca ggtgcgtaca cgctacgggg
     2641 gaagtgaggc tgaaccccgc acaccaacgc aggccgatcc gcgttccatc gtcgctcagt
     2701 cgagattaga agaggatccg cggctgaagc tcatgtgggc ggggtatgca ttttcgaaag
     2761 actttcgcga agaacgcggt cacgcctaca tgcttgaaga ccagatgttc acggagcggc
     2821 aacaggtaac gaaacaaccc ggtgcttgta tcaattgtca cgcttcggtg tatgtcgcct
     2881 acaagcagct cggcggcgag gacctttata agggcttcga tcagttgaac cggatgactt
     2941 acgccgaagc tcgcaaattt gtcacgcatc ccgtttcctg tattgactgt cacgattcac
     3001 agacgatgca actgcgagta acacgccttg cgttcatgga ggggatgcgc gtgtttaaag
     3061 cctcgcaggg tattcagaac tacgacgtga acgatcaggc cagtcgtcag gagatgcgct
     3121 cgtttgtctg cggtcagtgc catgatgagt actatttcag cggtccggag aagcggctcg
     3181 tgtatccctg ggctaagggt cttcaagtgg agaacattct cgccttctac gacgagaaga
     3241 agttcaaaga ctgggaacat gccgacaccg gtgcacccgt gctcaaggcg cagcacccgg
     3301 agtttgagat gtggaaccag gggattcacg cccgctctgg ggtagcctgc gccgactgtc
     3361 acatgcccta caagagggaa ggcgcgctta agatcagtga tcaccacgta cgctcaccgc
     3421 tgctgaacat caaccgtgcc tgccagacct gccataaatg gtcggaggat gaattaaagg
     3481 ccagagtcga aaccattcaa gggcggaccc acaatctccg caacctcgcg atggacgctc
     3541 ttgtcgatct gattaccgat atcaaggcag cccgaagtgc tggtcggaca gatgccgcag
     3601 taacaactgc ccaggacttt cagcgacgtg cgcagttcta cctcgacttc attgagtcgg
     3661 aaaactcaaa tggcttccat gctccgcagg aagccacgcg catactggga gaatcaatta
     3721 acttctcgcg gaaagggcaa gtcgctttgc gcagcagcgt gcaagcggcg ggtcagtgac
     3781 ggtagtatct tgtgtaaagc gccgcaggcg cggcattcaa tagccgcggc cgtaaggccg
     3841 tggattaaaa cccctaaatg gggtcgaggc ccgcagggcc gtcactcttt tgcgctgtga
     3901 atgggatcta gtgtcgcacc ttcggcgctc gaccattgtc tttcgactat cccacggcct
     3961 cacggccgtg gctatttact gacaggtcct tcggacctta attaggaatg ttttttgtgc
     4021 acat
//
LOCUS       DASUEX010000186         2239 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1404994, whole genome shotgun sequence.
ACCESSION   DASUEX010000186 DASUEX010000000
VERSION     DASUEX010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1404994"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..1309)
                     /locus_tag="VFV61_03095"
     CDS             complement(29..1309)
                     /locus_tag="VFV61_03095"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEX4899489.1"
                     /translation="MKSLRASRALLPLLLPFLAFGCNRNVSTSSSTKTLSSAASLTIH
                     AGTSPAGADSREPELSATTDGRMILSWVEKVGSKRYALRFATRDAGNWSTARTVAEGD
                     NWFVNWADFPSVIALTDGSLAAHWLVKSGTGTYAYDVNIARSKDGGNTWGKAIIPHTD
                     QTQTEHGFVSLLPLADGRVGAVWLDGRNLKDVKGEGDEHMPLPVSMTLRYAAIDVAGK
                     LSDEAQLDERVCECCQTSAALTSEGAIAVYRDRSDKEVRDIYYVSRQQGNWTTPRPVH
                     ADNWEISGCPVNGPAVAADGRRVAVAWFTGAKDSPRVKIAFSADAGASFAGPIDVDDG
                     ETLGRVDVLLLPDGSALVCWLSGTVEGGAVKVRRVSPDGMLGAPAVVAKTDISRSSGF
                     PRMARLGDEVHVAWTEFGKPSYVRTATVNVGDYK"
     gene            1512..1976
                     /locus_tag="VFV61_03100"
     CDS             1512..1976
                     /locus_tag="VFV61_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899490.1"
                     /translation="MHRRFLAATFVATALLFSQGGSFLVAALCPHMQSGTASCMSQSA
                     EPTMSHEDMGHRDHMQMEQEPKSQPDSSASALSKPASPCPHCAVHSGTTPRSASLRET
                     EVAKRSLDSGIPLQVPEVVPVAPATVAVLISRAHGPPGDPTPRHILINVFRI"
BASE COUNT          488 a          649 c          600 g          502 t
ORIGIN      
        1 gttagaaagt aattttcgca gacccctatt atttgtaatc tccgacatta acggttgcgg
       61 ttcgtacata tgaaggctta ccgaattcgg tccaggcgac atgcacttcg tctccgagtc
      121 gggccattct tggaaaaccg cttgaacgcg agatgtcggt tttagcgaca acggctggag
      181 caccaagcat tccatccgga cttacgcgtc gcaccttaac tgccccgcct tccaccgtcc
      241 ctgaaagcca gcaaacgaga gcagatccgt ccggcaacag cagaacatcc actcgtccaa
      301 gtgtttcacc atcatccacg tcgatgggac cggcgaaact cgcgccggca tcggcagaga
      361 acgctatctt gactcgcggc gaatctttgg cccccgtaaa ccaggctacc gccacgcgcc
      421 ggccgtctgc ggcgaccgct ggcccattca ccgggcagcc gctgatctcc cagttgtccg
      481 cgtggacggg ccgaggcgta gtccaattgc cttgctgacg acttacatag tagatgtcgc
      541 gcacttcctt gtcagagcga tcacgataga ctgcgatcgc gccttcggag gtcaatgcgg
      601 ccgaggtttg gcagcactca cacacgcgtt cgtcgagttg ggcctcatct gagagtttgc
      661 cggcgacatc aatcgctgca taacgcaatg tcatgcttac gggcaaaggc atgtgctcat
      721 cgccttcacc tttaacgtct ttgagattgc gaccgtccaa ccacacggca ccgacacgac
      781 catccgccag cggcagcagc gagacgaaac cgtgctcagt ctgcgtctgg tctgtatgcg
      841 gaattattgc cttgccccag gtattacccc catctttaga cctggcgatg ttaacgtcgt
      901 acgcgtaggt ccctgttcca ctcttcacca gccagtgtgc tgccagcgag ccatcggtga
      961 gcgcaatcac cgaggggaag tcggcccagt taacgaacca gttgtctcct tccgccactg
     1021 tacgagccgt cgaccagtta ccggcatcgc gtgtcgcaaa gcgtagcgcg tatcgcttac
     1081 tgcctacttt ctccacccag ctaagtatca tccggccgtc ggttgtcgca ctgagttctg
     1141 gctcgcggct gtcggcgccc gcaggggaag tgccggcgtg gatcgtaagt gaagctgctg
     1201 agctaagggt ttttgttgaa gacgaagtgc tgacgttgcg gttacaaccg aaagcgagaa
     1261 acggcagcag caatggcagc aaggcgcgtg acgcgcgcaa cgatttcata tcgagacaaa
     1321 ttcccccact gaagtccagc tgactaaaag actgagggat attacagcgt cgccgcttgc
     1381 tttagttagt cggcattcga cgcgtagcgt cgaggtgaat ttagcccggc ctttcaagga
     1441 cgcttgggcg gctccctagc catctgctat attcaagcct caagtgcaag acgtaaatga
     1501 cctgtatcac gatgcatagg cggttcttag cagccacttt cgtggcgaca gcgttgctgt
     1561 tcagtcaggg aggcagtttc ctggttgctg cgctctgccc gcacatgcag tcgggaacgg
     1621 cctcatgtat gtcacagtct gctgaaccca cgatgtcgca tgaagacatg ggacacaggg
     1681 atcatatgca gatggagcag gagccgaaat ctcaaccgga tagcagtgct tccgcattaa
     1741 gcaagccggc cagtccgtgc ccacactgcg ccgtacattc aggcactact ccgcgcagcg
     1801 cctcgcttag agaaacggag gtagcaaagc gttcgctcga ctctggtatt ccgctccagg
     1861 ttcccgaagt tgttccagtt gctcctgcta ccgttgcagt tttgatttcc agagcgcacg
     1921 ggccaccggg cgatcccact cccaggcata tcctcattaa cgtcttccgc atttagtacc
     1981 ttccgacaat cacattcgtg atagcgacgc aggcgtgtgc cttgtgttgc ctctattcat
     2041 tcaagcttga gctagaactt agtcagactt tagtttggcg aaccaactaa ggggggatcg
     2101 tgaatctgtc aggtaatttc aggattgcgc tgcgaacaat tagtgcattg gcactagtgt
     2161 ttgcgctcgg cgtgagtgct ttgccgcagc atgaaggtca tgacatgtca aaaatgaaag
     2221 cggcaagtaa gccgaagac
//
LOCUS       DASUEX010000187         3715 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1068029, whole genome shotgun sequence.
ACCESSION   DASUEX010000187 DASUEX010000000
VERSION     DASUEX010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3715
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1068029"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..1449)
                     /locus_tag="VFV61_03105"
     CDS             complement(67..1449)
                     /locus_tag="VFV61_03105"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX4899491.1"
                     /translation="MNDQTPAKETVLVVDDDRSIRSGLFWALSSTYRVLEAESGNVAL
                     DFLQRETIDVVVSDLHLPPHAEDISEGLAIIKAAHDREQPIPVVVITGSNSRAVALEA
                     VRKGAHGFFEKPLDEAEVLHVVNQAARMRRLELENARLREELPRSPSFSRLLGGSPSL
                     ERIVRDAHAVAATSATVLLTGENGTGKELLARAIHEESSRSAGPFVAVSCAALPEALI
                     ESELFGHEKGSFTSATYSRKGRFELANRGTLFLDEIGELTLSVQVKLLRVLQERAFER
                     VGGTKTLNVDIRLIAASNRDLEDEVEAGRFRRDLFYRLNVVPLVIPPLRNRREDIPLL
                     AAHFAAKTAEKYGRQTPELDSDLLEALLEYDWPGNVRELENLIERVVVLTGGHKLTTE
                     FLPEKMWRVLPDTNVADENTFEGGVLAVKRKLILSALESVGGNKVAAAKRLGISRSYL
                     HRLISEFGIQ"
     gene            complement(1530..3488)
                     /locus_tag="VFV61_03110"
     CDS             complement(1530..3488)
                     /locus_tag="VFV61_03110"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX4899492.1"
                     /translation="MFLRPRLFLIFVLACTAPLLLVGATSFLMNLRNARQTLAIELQE
                     ELAGAKYALHELLQTRQQDLLTLAGNKALVDFAEQQEKVELSALNGPATILTVGGAEQ
                     PVKDLQDALAPMLLNEKSYAAIAYFDSKRRPRFIAQKPTGPSDQSLIIRTRGFLEHES
                     EPDARAWSTKRGESVCSIVSLASVGKTLRCATSVYQDADAQGSHGILVTDTRLDSLVA
                     QVARTSEISASDQNRASPLAAVIVVLDRTGEIVYHTNDALKHQQVETSLPYFGPIATA
                     MVAGQSGQNFYRSTQGDEWLAAYAPIEPQGLSLAAARNYSLFTIALRRAGWFCLALAG
                     LFGISLAGVLTWFYQRRLQSLERVTEGVTAIAKGELDLHIDARSRDDIRQLAEGVNLV
                     TERLREQLAREAETRQIESFVRLSAMLTHDLKNAIGGLSLLVSNMEKHFNNEAFRADA
                     MRSLTDATEKLQSLVERLSNPITTLSGEYRRPQSVDVVPIVKKAVSQMVGQSGEGETG
                     SAISKHEVEVHFPDSLVALVDGERIEKVFENLILNAVEAMADKPGKLTITGGVEEEGK
                     IWVTVTDTGIGMSSRFIEEKLFRAFATTKKKGMGLGLYTCREVVRAHGGSIEVRSQPG
                     VGTTFRVVLPSAPPEGSIDKLTLNNSRS"
BASE COUNT          851 a         1083 c          938 g          843 t
ORIGIN      
        1 cttgagacct gaaacttgag acttgagacc tgagacttga gacctgaaac ttgagacttg
       61 agactactat tggattccaa actcactaat caagcgatgc aggtatgagc ggctgattcc
      121 cagtcgcttg gcggcggcaa ccttattgcc acccacgctt tccagcgcac tcagaatcag
      181 ttttcgcttc acggccaata ccccaccttc gaatgtgttc tcatccgcga cattcgtatc
      241 gggcagcaca cgccacatct tctcgggcag aaactccgta gttagtttgt ggccgccggt
      301 cagcaccacc acccgctcga tcagattctc caactcacgc acgttgcccg gccaatcgta
      361 ttccagtaac gcctcaagca agtctgaatc gagctcagga gtttgacgac catatttttc
      421 cgcggtcttc gcggcaaagt gcgccgccaa aagcggtatg tcttcccggc ggtttctgag
      481 cggcgggatc accagtggca ccacattcaa tcgataaaag aggtcccgcc ggaaacgacc
      541 cgcctccact tcatcttcca ggtcgcgatt gcttgcggca attagtctga tatccacgtt
      601 cagcgtctta gtaccaccga ctctctcgaa cgctctctcc tgcaacacac gaagcagttt
      661 cacctgcacg ctcagcgtca actcgccaat ctcatccaga aacaaagtgc cgcgattggc
      721 taattcaaaa cggcccttgc gcgagtatgt ggccgaagtg aatgagccct tttcgtgacc
      781 aaacagctca gattcgatga gcgcttcggg caaagctgca cagctaaccg ccacaaaggg
      841 gccagccgag cgggaactct cttcgtgaat tgctcgggcc aacaactctt tgcccgtgcc
      901 gttttcaccg gtgagtaaaa ccgtcgcact cgtagccgca acggcgtgcg catccctgac
      961 aattctttcc agcgacggac tgccacccag tagccgacta aaactggggg accttggtaa
     1021 ctcctcgcgc agtctcgcgt tttccagttc cagccgccgc atgcgggccg cctgattcac
     1081 aacgtgaaga acctctgcct catcaagcgg tttttcaaag aacccatggg cgcctttcct
     1141 tacggcctcc agggccaccg ccctcgaatt gctgccagtg atcaccacca ccggaatcgg
     1201 ctgttctctg tcatgcgcgg ctttgattat tgccaggccc tccgaaatgt cttcggcgtg
     1261 aggaggcaga tgcagatcac tgacgaccac atcgatggtt tcgcgctgca aaaaatcgag
     1321 cgccacattg cctgactcag cttcgagcac ccgataggtc gaactgagag cccaaaacag
     1381 accgctacga atactccggt catcatcgac aaccaggacg gtttccttgg ctggagtttg
     1441 atcgttcata tgctcgtcgc gcaatacacc cagtgtcacg caaaacggtt acagcattct
     1501 tgttgtgggg cggaaggatc ggggcagcac taagaacggg agttgttcag agtcagttta
     1561 tcaattgaac cttcaggtgg agccgatggt agcacgacgc gaaaagtcgt accaacaccc
     1621 ggctgggaac gcacctcaat cgagccgccg tgcgctctga caacctcacg acacgtgtaa
     1681 aggccgagtc ccatcccctt cttctttgtg gtggcaaagg cgcggaagag cttctcctca
     1741 atgaagcggc tactcatccc gattccggtg tcggtaaccg tcacccaaat tttcccttct
     1801 tcctccacac cgcccgtaat ggtcaacttg cccggtttgt ctgccatcgc ttccacggcg
     1861 ttcagtatta gattttcgaa aaccttttcg atgcgctcac catcaaccag tgcaacgagc
     1921 gagtcaggaa aatgaacctc gacctcatgc ttgctaatgg cagatcctgt ctctccctca
     1981 cctgactgtc ccaccatctg tgaaactgct ttcttcacaa tgggcaccac atcgaccgat
     2041 tgcgggcgtc tatattctcc gctcagggta gtaatgggat tgctcagccg ttcaaccagg
     2101 gactgcagtt tttcagtggc atctgtaagc gatcgcattg catcggcgcg gaaagcttcg
     2161 ttgttgaagt gtttctccat gtttgaaacc agcagcgaca atccaccaat cgcattcttc
     2221 aggtcgtgag tgagcattgc tgacaatcgg acaaaggact ctatttggcg cgtctccgct
     2281 tcacgggcca actgttcacg caaacgttct gtcaccaggt taacgccttc cgccaattga
     2341 cggatgtcgt cacgcgagcg cgcatcgata tgcagatcaa gttcgccctt ggcaatggcg
     2401 gtcacacctt cggttactct ctccagactc tgcaatcgcc gttggtagaa ccaggtcagc
     2461 acgccggcga ggctgatacc gaaaagacca gccagagcca ggcaaaacca tcctgcccgc
     2521 cgaagcgcaa tcgtgaagag tgaatagttg cgtgccgcag cgagcgaaag gccctggggt
     2581 tcaattggcg cgtacgccgc aagccattca tcgccctggg ttgagcgata gaagttctgg
     2641 ccactttgac ccgccaccat cgccgtcgca attggaccaa aatacggaag ggacgtttcc
     2701 acctgttggt gtttgagggc atcgttggtg tgatagacga tctcgccggt gcgatctaga
     2761 accacgatca ccgccgctaa tggactcgct cggttttgat cgctcgcaga aatctccgat
     2821 gtgcgggcga cctgcgcaac cagggaatcc agtcttgtat cagtcaccag aattccgtgg
     2881 gaaccttgag cgtctgcatc ctggtaaacg gacgtggcgc aacgaagagt ctttcccaca
     2941 gaagccagac taacgatgct gcaaacactt tcaccgcgtt tcgtgctcca agcacgcgcg
     3001 tcaggttcgc tttcatgttc gaggaatccc ctggtcctga tgatcaaact ctggtccgaa
     3061 ggcccagtag gtttttgcgc gatgaagcga ggtcttcttt ttgaatcaaa gtaagcgatt
     3121 gctgcatagg acttctcgtt cagcagcatg ggcgcaaggg cgtcctgtag atctttgacc
     3181 ggctgttcag ccccacccac cgtaagaatg gtcgcgggtc cattcagggc actgagctcc
     3241 actttttcct gctgttcagc gaaatccacc agggctttgt tgccggccag ggtgagtaga
     3301 tcctgctgtc gggtctgcag caattcgtgg agagcgtatt tggcgccagc tagttcctcc
     3361 tgcagttcta tagccagcgt ttgccgcgca ttacgcagat tcatcaggaa gctagtcgcg
     3421 ccaactagta ggagtggcgc ggtgcatgcg aggacgaaaa tcaggaagag tcttggtctg
     3481 aggaacacag caagatctcc agcatccggt acccgtttag tgatattagc tcagtgcaag
     3541 atttccggta aggaagtttt gagtttcgga ctttgggagc gaaaccccag gtacagacga
     3601 aatatgaatt cgagcccgca aagccgcaag cataaagcct catggagcga ggtttggagt
     3661 tgagtttcaa gtccgtcaag actcgaaact caaaactcga aactcaaaac tcgaa
//
LOCUS       DASUEX010000188         7000 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_147033, whole genome shotgun sequence.
ACCESSION   DASUEX010000188 DASUEX010000000
VERSION     DASUEX010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7000
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147033"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..636
                     /locus_tag="VFV61_03115"
     CDS             <1..636
                     /locus_tag="VFV61_03115"
                     /inference="COORDINATES: protein motif:HMM:NF025314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4214 domain-containing protein"
                     /protein_id="HEX4899493.1"
                     /translation="GREPDPGGFQGWQDTINNCPSGNTTCDRIHVSGNFFQSPEFQAR
                     GYFVYRFYPVAFGRKPDYAEFIPDLARVSGFLSDTQLEAAKVAFINDFMSRPAFAATY
                     NGLSDTAYVDTLLTTAGVAPHQFRDFWIAALGNGTRTRATVLRDISESPEVYNKYYNQ
                     AFVVMQYFGYLRRDPDASYLNWIQELNTTGNSRNMINGFVNSLEYYFRFGP"
     gene            822..1997
                     /locus_tag="VFV61_03120"
     CDS             822..1997
                     /locus_tag="VFV61_03120"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEX4899494.1"
                     /translation="MRIALDGFPLSTPKTGIGHYTLQLARALAEISPADEFELISPFP
                     FSDSVIDELRQASLANLILNNPQAASFRRRWWALGLPLFLRKNSFDLFHGTNYEVPLW
                     NRRRNVLTIHDLSLLLHPEYHEPRLVRRSRRRLPLMIRSAAKIIAVSESMKREMVAHL
                     KINADRIVVTPEAPRQNFRPLPAAETIAIRERHGIEADFILAVGTIEPRKNLIALARA
                     LDHILRTTALRPQLVVAGGEGWLTEDLHSFIARSSCAERVRFIGYTSDDELGALYSSC
                     RISVYASLYEGFGLPPLEAMACGAPVIASRIPAIEETVGDVALLVDPHDVAELARAIV
                     QLWNDEELRAERSAAGLRRAAEFSWKKTASLTLDVYREVLRPLGLKAIKKRGQAAPS"
     gene            complement(2087..2791)
                     /locus_tag="VFV61_03125"
     CDS             complement(2087..2791)
                     /locus_tag="VFV61_03125"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX4899495.1"
                     /translation="MQNNVPDISIVIPALNERENLELLLPLLNEVINALNLKSEIIIA
                     DGGSADGTAELAQQQGAQVVMQTEPGYGGALLAGFASARAPYVVTMDADLSHPPVFLR
                     DFWEQRTTAQMLIASRYVPGGKADMGSSRRVLSAILNKTYARVLDLPLADLSSGYRMY
                     HRDLLAKLELRARDFDVLEEILVKVHLLGGRIKEVPFHYQARNSGKSHAKLFKFGWAY
                     LRTLARMRRLRNSTRN"
     gene            complement(3115..3963)
                     /locus_tag="VFV61_03130"
     CDS             complement(3115..3963)
                     /locus_tag="VFV61_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899496.1"
                     /translation="MKLAVTILLITLAASSAWAQAPTLRIVAPDPNLPAELYYGNTKV
                     KPLRLRPGTNTQITIDDADFFVFQHYVDFLNRMPEPGGYAGWQAVLNGCVPVTASCDR
                     IHVSGAFFQSPEFQERGYFAYRQYSVAFGRKPDFNEFMPDLRAVSGFLDANQLEAAKV
                     ALINNFMARPAFVSTYNGLANAPFVDLLVSTAGVVLPNKQALIDSLNAGTMTRAQVFR
                     AIMESPEVYAKYYNQSFVVMQYFGYLRRDPDALYVDWIAVLDQTGDPRHMINGFMNSL
                     EYRNRF"
     gene            complement(4298..>7000)
                     /locus_tag="VFV61_03135"
     CDS             complement(4298..>7000)
                     /locus_tag="VFV61_03135"
                     /inference="COORDINATES: protein motif:HMM:NF021126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEX4899497.1"
                     /translation="GAPLAVGADGQNLYLPNFLGRKVFWDHMIFAGFPMMADPQVMTW
                     YPPALLFSLIPGSWNPFILLAYVLTASFMYGYVLTVTESRLAALTSGVILGMSGFMMA
                     HLGHAVVIHAVAWVPLQLWSYEKLRHRWSAGWFVAGCVAIALSFFGGHTPIFTLGFGL
                     AAAYAVVLGWNAPIGRWRYYLTCGLMMVLGIGLVALQLLPTAEIVGESIRTGYKFSDF
                     ISHSLPPHQVLMLVFPKLFGGLKESGALPYYGAENLTELTGYVGLLPLLLAGIGVARW
                     PQRALSIFWLSVALVAVVLAMGDGTPLARLIYHVPVINQFRAPGRHLLEFTFAVCILA
                     SLGMASLLKHNATSALVFKVITLGAVVLIVGAVLVFTNLTSLEALAAHKDLSPEQVWR
                     WTRWAVAIPLALFVCSAVSLSYWVRRTKSLGRIVLLFAVLMIDLASFGWSYDWHYVGI
                     DKDALQVPEIATRYRYLLQGNHQRILPTRGTMAPRGEMPPNLSRLWGVPNASGYNSLV
                     LSRFSKLLNMQDVGTVTRPLWWDPDDQSLNLASVRYLLIPKMEVMTDQSGTSWQKTDL
                     QLWLGAGCNPTSTDSVTFNPPDPAPGTAIGIVARLACAVNIPDGTEVVRLQVTDVDNN
                     SESHSLLAGRDASDWAYDCETIKKSLKHSRAQIFDNFAAKVYEESCTGHYYRSNFQLS
                     GRKKIKSIEFRWTGPSGSMILDKISLIDNELHTSRPVDPMTMGQHWRFLEESDGSRVF
                     ENLNAMPRAWLVSDVLVLEAEEARTAGQTSRLPGGRAFDPKRTGLIERPLPMDIAPTD
                     TNATARVTALVDNTMEVRTDSAAAAFLITSDVYYPGWRVTIDDQPVELFRANYAFRGV
                     AVPAGQHVVKFTYHPRRFYLGVAVSLASLFVLGGLAVVSLARRK"
BASE COUNT         1650 a         1926 c         1925 g         1499 t
ORIGIN      
        1 ggtcgcgaac cagacccagg tggcttccag ggttggcagg acactataaa caactgtccc
       61 tctggaaaca cgacctgcga tcgcattcat gtctcgggaa acttcttcca gtcgcctgaa
      121 ttccaggcgc gcggttactt cgtctatcgc ttctatcccg tggcctttgg tcgcaagccc
      181 gactacgccg agttcattcc cgacctagca cgcgtcagtg gctttctcag cgatacgcaa
      241 ttggaagcag caaaagtggc gttcatcaat gacttcatga gccgacctgc gtttgcggcg
      301 acttataacg gactcagcga tacggcgtat gtggacacgc tgctaacgac agccggcgtt
      361 gcgccccacc agtttcgcga cttctggatc gcggccttgg ggaatgggac acggacaaga
      421 gcgacggtgc tgcgcgacat ttctgagagt cccgaggttt acaacaagta ctacaaccag
      481 gccttcgtgg tgatgcagta ctttggttac ttgcgccgtg atccggatgc ttcgtacttg
      541 aactggatcc aggaactgaa cacgacgggc aactcacgaa acatgatcaa tggttttgtg
      601 aattcactcg agtattactt ccgcttcgga ccctaggaag attgccaatt ggtgaatgcc
      661 aattgccgat ttgtgacaac tctgtcaggc aattcgcagt ctttgcaatt ttagtcggaa
      721 ggcgagcttg cctccgctaa gtgtaagtaa cactcaacca acgggggtaa cacccttggg
      781 cggttctgaa ataatcggca atcgccaatt ggcaatcggc aatgagaata gcgctcgacg
      841 gctttccgct gtcaactcca aaaaccggca tcggtcatta caccctgcag ttggcacgtg
      901 cccttgccga gatctcgcca gcagatgagt tcgagctgat ctcaccattt ccctttagtg
      961 attccgtcat cgacgaactc cggcaggcgt ccctggcgaa cctgatccta aacaatccgc
     1021 aagcagcttc atttcgccgc cgttggtggg cgctgggact gccattattt ctgcggaaaa
     1081 attcgttcga cctctttcat ggcactaact atgaagttcc actttggaat cggcgtcgca
     1141 atgtgctcac tattcacgat ctctcactcc tgctccatcc cgagtatcac gagccacgtc
     1201 tcgtgcgacg ctcccggcgt cgcttaccct tgatgattcg ttcagccgcc aaaatcatag
     1261 ctgtctcaga aagcatgaaa cgagagatgg tcgcgcatct aaagatcaac gccgaccgaa
     1321 tagtggtcac gccggaagca ccgcgccaaa atttccggcc gctgccggcg gcagagacga
     1381 ttgcgatcag ggaacgacac ggaatcgaag cagacttcat tctcgccgtc ggtaccattg
     1441 agccacgaaa gaacctgata gccctggccc gcgcgctcga tcacatcctg cgcactacag
     1501 cgctgcgccc gcagttggta gtcgccggcg gtgagggctg gctgacggag gaccttcatt
     1561 cgtttattgc ccgaagcagt tgcgctgagc gcgtccggtt tattggctac acttcagatg
     1621 acgagttggg cgctctttac tcatcttgcc gcattagcgt ttacgcgtct ttgtacgaag
     1681 gatttggttt gccaccgctg gaggcaatgg cgtgcggggc ccctgtgatt gccagtcgga
     1741 ttccagcgat cgaggaaaca gtgggagatg tggccttgct agtagatccg cacgatgtgg
     1801 ctgagctggc gcgcgccatc gtgcagcttt ggaacgacga ggagctacgc gcggaacgat
     1861 ctgcagccgg actgcgtcgc gcggccgagt tcagctggaa aaaaacagcg agtctgactc
     1921 tggatgtgta tcgagaggtg cttcgtcctc ttggattgaa ggcgattaag aagcgggggc
     1981 aagccgcccc ttcctgactg agcattttgt gacttgagag tcttggccct catgctcctg
     2041 gtcaagcagc agagccaatc ggcaatcgac aatcggcaat tggcaatcag ttgcgcgttg
     2101 agtttcttag gcgcctcata cgagcgagag ttctgagata agcccaacca aacttaaata
     2161 gtttcgcgtg cgactttccc gaatttcttg cctggtaatg gaaaggcact tccttgattc
     2221 tcccgcccag caagtgaacc tttaccagta tctcctcgag cacatcgaaa tcgcgggccc
     2281 gtagctcgag ttttgccagc agatcgcggt ggtacattcg atagccgctg gagagatctg
     2341 cgaggggcag atcgagcacg cgcgcgtagg tcttattgag gatggcacta agcacgcgac
     2401 gagaagatcc catatcggct ttgccgcccg ggacgtaacg cgaagcaatt agcatttgtg
     2461 cggtggttcg ttgctcccag aagtcgcgca ggaagaccgg agggtgagac aagtctgcat
     2521 ccatcgtgac gacatagggc gcgcgagcgg aagcgaaacc cgccagtagc gcgcccccat
     2581 aaccaggttc ggtttgcatc actacctggg cgccttgttg ctgcgcgagt tctgccgtac
     2641 cgtccgcgga acccccgtcc gcaatgataa tttccgattt gaggttcagt gcgttgatga
     2701 cctcgttcaa gagaggcagc aagagttcca ggttttcgcg ctcattgagc gcgggaataa
     2761 ctattgaaat gtcgggaacg ttgttctgca ttagaatata gatgtttgct tgaaactcta
     2821 ttctgaatgt tcgtgcggct tcgccgcttt ccgtgcggta agccttcggc ttaccgacta
     2881 caataccctc gctgcgaggc tatgcctcgt ccatctcctg taagttcaat ccgaaagccg
     2941 aggcgcagcc tcagacaaga agctcctcgc cgggaagcca gaggcttacc gcccggaaag
     3001 cggcagagcc gcagttatga aaacgtagag gcgtttgcag ttaattatct acatgcaaac
     3061 acaaaaaagg ccaactgaaa gcagttggcc ttttatttca gctggtcggg tctcttagaa
     3121 gcggttgcga tactccaggg agttcatgaa accgttaatc atgtgtcgcg gatccccggt
     3181 ttgatccagc accgcaatcc agtccacata tagcgcatcc gggtcccggc gcagatagcc
     3241 gaagtattgc atcactacga aagactggtt gtagtacttc gcatacacct cggggctctc
     3301 cataatcgcg cggaacactt gcgcccgcgt catcgtgccc gcattaagcg aatctatcaa
     3361 cgcttgctta ttcggtagaa caacacccgc cgtcgacacg agcaggtcaa cgaagggagc
     3421 gtttgcgagt ccgttgtaag tgctgacgaa agccgggcgc gccatgaaat tgttgatcag
     3481 cgcgaccttc gcagcttcca gttgattggc atcgagaaat ccgcttacgg ctcgcaggtc
     3541 aggcatgaac tcgttgaaat ccggcttgcg gccaaaggcg accgaatact gacggtaggc
     3601 aaagtatccg cgctcttgaa actcaggcga ctgaaagaag gctcccgaaa cgtggatgcg
     3661 atcgcagctg gccgttacgg gcacacaacc attcagcaca gcctgccagc cggcatagcc
     3721 acccggctcg ggcatgcgat tcagaaagtc gacgtaatgc tggaagacaa agaagtcggc
     3781 gtcgtcaatc gtgatctggg tatttgttcc gggacgcaaa cgcaggggtt ttaccttcgt
     3841 gtttccgtag taaagctcgg cgggaagatt cgggtccgga gcgacgatgc gcaacgtagg
     3901 tgcctgagcc caagcgctcg acgcggcgag ggtaatcaac agaatagtaa cggctaattt
     3961 catttttgct gctcctacct aagtttcatt agcggaaaag agagccgggt tgaggtgttc
     4021 tctttaggag gtgccacgcc acccaggggg caccatcgaa tatagcccgc cgccgcaatt
     4081 agtgtcaatt agatcatttc tcaggggtga ccgtagggca gacagtttgc gttacttccc
     4141 ctggccagcc cttttgcgaa accggatttc cgttcaggtt gaccggcgtt gccacagcca
     4201 gatgggcgcg gagagccacc tttcagcttg ccagccaaga tatatagacg ctattcctat
     4261 cacgaaagtt ggactttgat tctgtttttg tccgggttta ctttcgacgg gccagactca
     4321 cgactgccaa gccgccaagc acaaatagcg aggcgagact tacggcaact cccagataga
     4381 agcggcgcgg gtggtaagta aacttcacta catgttggcc cgcgggaacg gcgacgcccc
     4441 ggaaggcata gttagcccgg aaaagctcga ctggctgatc gtcaatggta actcgccagc
     4501 cagggtagta aacgtcactg gtaatcagaa aggcagcagc agccgagtct gtacgtactt
     4561 ccatggtgtt gtccaccagg gcagtcaccc gcgccgtcgc gttcgtatcc gtcggcgcaa
     4621 tgtccatggg cagcggcctc tcgatcaacc cagttcgctt cggatcaaag gcgcgaccac
     4681 cgggcaacct ggaagtctgg cctgcagtgc gagcttcctc agcttcgaga accaggacat
     4741 cggaaactaa ccaggcgcgc ggcatcgcgt ttagattctc gaaaactcga gagccatcgc
     4801 tctcttctaa aaagcgccag tgctgaccca tcgtcattgg atcgaccgga cgcgaagtgt
     4861 gaagctcgtt atcgatgaga ctgattttgt ccaggatcat ggagccggaa ggaccggtcc
     4921 agcgaaactc gatactcttg atctttttgc gtccgctcaa ttgaaagttg gatcggtagt
     4981 agtgacccgt gcatgattct tcgtagacct tggctgcgaa gttatcaaaa atctgtgcgc
     5041 gactgtgctt aaggcttttt ttgattgttt cgcagtcgta agcccaatcg cttgcatccc
     5101 ggccagcaag caaactatga ctctcggaat tgttgtccac gtcggtaact tgcaaacgta
     5161 cgacttcggt gccatcagga atgtttacgg cgcaggcaag gcgagcaacg attccaatcg
     5221 ccgtgccggg agcggggtcg gggggattaa aagtaaccga atcggtcgac gtggggttgc
     5281 aaccagcgcc gagccagagc tgcagatctg ttttttgcca cgacgttccc gattgatcag
     5341 tcatcacctc catcttcggt atcaggagat aacgaacaga cgctaagttc agactctgat
     5401 cgtcaggatc ccaccacagc ggccgggtca ccgtgcccac atcttgcatg ttcagtaatt
     5461 tgctgaaacg tgaaaggacc agggaattgt atccgctagc attgggaact ccccacaagc
     5521 gcgacagatt gggaggcatc tcaccccgcg gcgccatcgt tcctcgggtc gggagtattc
     5581 gttgatggtt gccttgcagc aggtagcggt agcgagtcgc aatttcaggg acttgaagcg
     5641 catccttgtc gattcccacg taatgccaat cgtaggacca gccgaagctg gcaagatcga
     5701 tcataagcac cgcaaacagg agaacaattc tgccgaggga ttttgtccgc ctgacccagt
     5761 aactcaacga cactgctgag caaacaaaaa gagcgagcgg gatagcaact gcccatctcg
     5821 tccaacgcca gacctgttct ggagataaat ccttatgagc ggccagggcc tcaaggctcg
     5881 tgagattagt aaagacgagc accgctccca ctatcagcac aaccgctccc agggtaatga
     5941 ctttgaaaac aagcgccgac gttgcattgt gttttagcaa gcttgccatc cccaaactgg
     6001 ccaggatgca aacagcaaaa gtgaactcca acaagtgtct tccgggggcg cggaattgat
     6061 tgattaccgg aacgtggtag atcaggcgag ccaggggagt cccgtctccc attgccagca
     6121 ccaccgcaac cagtgcaacg cttagccaga agattgagag cgcccgctgt ggccacctgg
     6181 ccacaccaat acccgccagc aaaagtggca gcaagcccac atatccagtc agctctgtta
     6241 ggttttctgc gccgtaatag ggaagggcac cggattcttt cagtccccca aacagtttgg
     6301 gaaagacgag catcaagacc tggtgcggtg gcagagagtg agaaatgaaa tcggaaaatt
     6361 tatagccagt gcggatgctt tccccaacaa tctcggccgt gggcaggagc tggagagcga
     6421 ctaggccaat tcccagaacc atcatgaggc cgcaagtcag gtaataacgc caacgaccaa
     6481 ttggcgcgtt ccagccgagt acgaccgcgt aggctgcagc tagtccgaac cccagggtga
     6541 agattggagt atgacctcca aaaaagctca gtgcgatcgc gacgcagccg gcaacaaacc
     6601 atccagcact ccacctgtgg cgcagctttt cataagacca cagctgcagg ggaacccacg
     6661 cgactgcatg aatcacgacg gcgtggccca ggtgcgccat catgaaaccg ctcatcccaa
     6721 gaatcacacc gctggtgagt gcggccaatc gcgattcagt aacggtcagc acatagccgt
     6781 acatgaacga cgccgtcagg acgtaggcga gcaggatgaa cggattccag gaccccggaa
     6841 tgagtgagaa gagaagtgcc ggcggatacc acgtcattac ctgcgggtcg gccatcatgg
     6901 gaaagccggc gaaaatcata tgatcccaaa aaactttgcg gcccagaaaa tttggcaagt
     6961 agagattctg gccgtcggcg cctacggcga gtggcgcgcc
//
LOCUS       DASUEX010000189         2739 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1068516, whole genome shotgun sequence.
ACCESSION   DASUEX010000189 DASUEX010000000
VERSION     DASUEX010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1068516"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            126..1679
                     /locus_tag="VFV61_03140"
     CDS             126..1679
                     /locus_tag="VFV61_03140"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HEX4899498.1"
                     /translation="MPATQIPENDMRIAPANLRELLRQRSAATPEKTFLFSENDDRRY
                     TYAEFEAAVFRAAALLASQGIGKGDVVSLLLPNSVEYLVGYFACWSLGALAGPVNSLL
                     KAEEIAYVMSNSEAKAILVHTDFQPTIESIRSELPSLNAVIPFDDEAEATRNSGGANS
                     PVIELNRADEAIIIYTSGTTGKPKGCLLTHGNVIANARQISKWLSFTAADRLLTIMPL
                     FHMNAVSVTTMSALYAGGSTVVSPKFSASRFWQIISDYQITSFGSVATMLSMLLSTYP
                     DGVPVGLKTDQLRFAMCGSAPVPAETMKRFEETFNCLVIEGYGLSESTCRSTFNPPDQ
                     RRRAGSCGIPIGNEMKVVDDHDNEVPDGQLGEIVMRGENILKGYYKNETATATAFRNG
                     WFHTGDIGYRDADGFYFIVDRKSDMIIRAGENIYPREIDEVLYQHPAVAAAATIGVPD
                     SLYGEEVAAFIVLKDGASCTAADLIDFCRVHLADFKCPKSIRIVAEIPKGPTGKLLKR
                     KLAKEFAPQ"
     gene            complement(1745..2587)
                     /locus_tag="VFV61_03145"
     CDS             complement(1745..2587)
                     /locus_tag="VFV61_03145"
                     /inference="COORDINATES: protein motif:HMM:NF015213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HEX4899499.1"
                     /translation="MFNSFIIVLREGFESFLLVAVILTYLRKAGHKWLTSAVYVAILL
                     GLGISAGLGYVLKNGVEEVTILQTFGTTAGPYVNQFLANEALREGVLGIVAIVMVATL
                     VVHMWRSGPQLQEKMRARLGVMSSRKSRIAAVAGVFLFTFLMITREGMETALLLLQVR
                     DQQMITGALLGLAAALVFAWGWARYGHLINIKRFFRVTGIFLLLFMLQVAIYSFHEFS
                     EAGLLPNSETLHTATEKFSPDGLYGKWFSLLMVAVCGLWLFGGWLIDRLKPTHNTTIR
                     LGET"
BASE COUNT          690 a          713 c          733 g          603 t
ORIGIN      
        1 caaacttagg tttgtcgttt gtcattactg ataagaagag atggggcaaa agcgacggac
       61 tagagttgtc agacgaacaa gtggatgact ctagcgagcc cgtaatatac catacgctct
      121 agaacatgcc agccacccaa atacccgaaa acgacatgcg aattgcgccg gcaaacctca
      181 gggagctgct caggcagcgc agtgcggcca cgcccgaaaa aacttttctt ttttccgaga
      241 acgatgaccg gcgctatact tatgcagagt ttgaagcggc cgtctttcga gccgccgcgt
      301 tgctcgcgtc gcaagggata ggaaaaggcg acgttgtcag tctgctgctc ccaaatagcg
      361 ttgaatacct cgttggctat tttgcctgct ggagtttagg cgcgctcgcg ggtccggtaa
      421 attcgctttt gaaagccgag gaaattgcct atgtcatgtc taactcggaa gccaaagcga
      481 ttctcgtgca cactgatttc cagcccacca ttgaaagtat ccggagcgaa ttgccgagtc
      541 tcaacgcagt aattcctttc gacgatgagg ctgaagcgac caggaacagt ggtggtgcga
      601 attcgcccgt aattgaactc aatcgcgctg acgaggccat cattatttac acctcgggaa
      661 ccacgggcaa accgaaggga tgtttgctca cgcacggaaa cgtgattgcc aatgcgaggc
      721 agatcagcaa gtggcttagc ttcacagcag cagatcgctt gttgacgatc atgccgctgt
      781 ttcatatgaa cgctgtatcg gtgaccacta tgtccgcgct gtatgccggc ggctcaacag
      841 tggtgagtcc aaaattctcg gcctctcgtt tctggcaaat catctctgat tatcagatca
      901 cttccttcgg ctcggtcgct acgatgttgt cgatgctcct cagcacttat cccgatggcg
      961 ttccggtggg cttgaagact gatcagttgc gcttcgcgat gtgtggttcg gctccggtac
     1021 ccgcggagac tatgaaacgc tttgaggaaa cgttcaattg cctggtgatt gagggctatg
     1081 ggctgtcgga atctacgtgt cgctcaactt tcaacccacc cgaccaacgc cgtcgcgccg
     1141 gctcttgtgg cattcccatc gggaatgaaa tgaaggtagt agacgaccac gataatgaag
     1201 tgccggacgg tcaactcggt gaaattgtga tgcgtgggga gaacatactc aaaggttatt
     1261 acaagaacga gaccgcgaca gcgacggcct tccgcaatgg ctggttccat acgggcgaca
     1321 tcggctaccg cgatgccgac ggtttttatt tcatcgtcga tcgcaagagc gacatgatta
     1381 ttcgcgcggg agaaaacatc tatccgcggg agatcgacga agtcctctat caacatccgg
     1441 cggtggcggc ggcggcaacg atcggcgtcc cggattcact ctatggtgaa gaggtggcag
     1501 ctttcatagt cttgaaggat ggcgccagtt gcaccgcagc agacttgatt gatttctgcc
     1561 gagtacattt ggcagacttc aagtgcccca aatcaatccg cattgttgca gagatcccta
     1621 aaggaccgac cggcaagctg ttgaagagga aattagcgaa agagtttgca ccgcaataag
     1681 ccatccggga aacaggaaag gcgcggcctt atttaaccgc ctcttgtttc ggaagtttgt
     1741 aagcttaagt ctcacccaat cgtatggtcg tattgtgagt cggcttcaaa cggtcaatta
     1801 gccaacctcc aaacaaccag agcccacaaa cggcaaccat caacagggaa aaccattttc
     1861 cataaaggcc gtcaggcgaa aacttctccg ttgcggtgtg aagtgtttcg ctgtttggaa
     1921 gcaggcccgc ttccgagaat tcgtggaacg aatagattgc cacctggagc atgaatagca
     1981 ataggaagat tccggtaacg cgaaagaacc gcttaatgtt tatgagatgt ccgtaacgcg
     2041 cccaacccca ggcgaagacc agtgcggcgg ccaacccgag cagggcaccg gtaatcattt
     2101 gttggtcgcg cacctgcaac aacaacaatg ccgtttccat gccttcgcgc gtgatcatca
     2161 aaaaggtaaa caggaagacg ccagccacgg cagctattcg cgattttctc gatgacatca
     2221 ctccgagccg agccctcatc ttctcctgga gctgtggtcc gctacgccac atgtgcacaa
     2281 ccagcgtcgc caccataaca atcgctacta tgccgagaac gccttcacga agtgcttcgt
     2341 ttgccaggaa ctgattgacg tacggtccgg ctgttgtgcc gaaggtctgc agaatagtca
     2401 cttcttcaac gccgtttttt aagacatagc cgagcccggc actgatcccc agccccaaaa
     2461 ggatcgccac ataaacggcg gaagtgagcc acttatgccc agctttgcgc aggtacgtca
     2521 ggatcacggc gacgaggagg aacgactcaa agccttcgcg gagcacgatt atgaatgaat
     2581 taaacataga gcggctttct tcagttggat ggcgaggaag ccaatgggcc tccagcacgg
     2641 gcgagagtat agcccaattg aaaatagatt tcaatttcat aagagggtcc aaacgaaggc
     2701 ggggcgagga gcggacctta ctgcgaggtt aggggaata
//
LOCUS       DASUEX010000190         2608 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1280519, whole genome shotgun sequence.
ACCESSION   DASUEX010000190 DASUEX010000000
VERSION     DASUEX010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1280519"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..888
                     /locus_tag="VFV61_03150"
     CDS             <1..888
                     /locus_tag="VFV61_03150"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEX4899500.1"
                     /translation="DAAINRGNSGGPLVNMRGEVIGINSQIATSTGDYNGIGFALPAS
                     EASFVYQQIVSQGKVKRGYLGITLDSVKDEFAKVYGLNETRGAIVMDVQQTRDGQPTP
                     AAKAGIQSNDIIVEFNGQPVVSAQDLITKVAGSPVGQAAVLGYLRDIEGKLEKRTASV
                     VLGERPGLQVSKSSDPTTPPKDPNAKGNLPHLGITLQEITQQLIAEKHLTGVRGLYVK
                     EVDPNGLAADVRLAGGVPAVGEGDVITRINRVPVTTLADFQRVLNGLKPGDPIVLNVS
                     RYQRDGDRIVQRIVQFTYQ"
     gene            973..2532
                     /locus_tag="VFV61_03155"
     CDS             973..2532
                     /locus_tag="VFV61_03155"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX4899501.1"
                     /translation="MAQAKKALARSSRKSNGSAPRTSTKTYSNYIGGKWVPSRSGEVF
                     ENLNPADTRDVIGRFPRSTSDDVNDAVNAARGAYDRWRHTPAPRRAEILFRLGEILIR
                     NKDAYSADMTREMGKVLKETGGDVQEAIDCTYYTAGEGRRLHGFTTPAEMPDKFAMCV
                     RQPVGLCGLISPWNFPMAIPSWKLIPALVCGNTVVIKPAEDTPLSTYNLVKACEEAGI
                     PPGVVNLVTGYGDPGAAMTNHPSLRLISFTGSTATGRLVASACAERNAICSLEMGGKN
                     VIMVMEDADIDLAIEGAVWGAFGTSGQRCTAASRVVVHKKVYKKFSQQLVERAKALRV
                     GNGANPKTDMGPVINNDAVDKIMGYVDIGQNEDGATLACGGKRLTRGEYAHGYFIEPT
                     VFTDVTPDMRIAQEEIFGPVTSVIPAKSLDEAVEIANGVRYGLSAAIYTRDVNRAFHA
                     MNEMYTGIFYVNASTIGAEVHLPFGGTKATGNGHREAGTQVLDIFSEWKSIYVDYSGK
                     LQRAQIDEVNL"
BASE COUNT          596 a          716 c          760 g          536 t
ORIGIN      
        1 gatgccgcta tcaatcgggg caacagcggc ggcccgctcg ttaacatgcg gggcgaagtg
       61 atcggcatca attcacaaat cgccacttcg actggcgatt acaacggcat cgggttcgca
      121 ctaccggcca gtgaagcaag ctttgtctat cagcagattg tctcgcaagg aaaagtgaag
      181 cgcggctacc ttggcattac actcgattca gtcaaagacg aattcgccaa agtttacggt
      241 ctaaacgaaa ctcgcggcgc gatcgtgatg gatgtgcagc aaacccgaga cgggcaaccg
      301 acgcctgctg ccaaggccgg aattcaatcc aacgacatca tcgtggaatt caacggtcag
      361 ccagttgtca gcgctcagga tttgatcacg aaagtcgcgg gcagtccggt gggtcaggca
      421 gcggtgctcg gttatctgcg tgacatcgag ggcaaactcg aaaaacgaac cgccagcgtg
      481 gtactgggcg aaagaccggg cctgcaagtc tcgaagagtt ctgatccaac aactccgccg
      541 aaggatccga acgccaaggg caacctgccg catctcggta tcacccttca ggaaatcacg
      601 cagcagctga tcgccgaaaa acatctcacg ggtgtgcgcg gtctctacgt gaaggaagtg
      661 gatcccaatg ggctggctgc ggatgtgcgg ctggcgggcg gcgtgccggc ggttggcgaa
      721 ggcgatgtca ttacacgcat caaccgggtg ccggtgacta ccctggcgga cttccagcgc
      781 gtgctcaacg gattgaagcc cggagatccg atcgtgctaa acgtttcgcg ttatcagcgc
      841 gacggcgacc ggattgtgca acgtatcgtt cagtttactt accaataaga tctcggtatc
      901 gggtgttggg tgtcgggttc cagaaagccc ggttcaaccg gcacccgata cctgtcacct
      961 gatacctgaa ttatggctca agcaaagaaa gcactcgcga gatcctctcg caaaagcaac
     1021 ggcagcgcgc cgcgcacttc gacaaaaaca tattccaatt acatcggtgg caaatgggtg
     1081 ccgagcagat ccggtgaggt ttttgagaac cttaatccag cggatactcg cgacgtcatc
     1141 ggccgctttc ctcgttctac cagtgatgac gtaaacgacg ccgttaatgc ggcgcgcgga
     1201 gcgtatgatc gctggcgtca tacacccgct ccgcgccgcg ccgaaattct tttccgtctc
     1261 ggcgagattc tgattcgcaa caaggacgcc tactccgcag acatgacgcg agagatgggc
     1321 aaggttttga aagagaccgg cggcgatgtg caggaagcaa ttgactgcac ttactacacg
     1381 gccggtgagg gccggaggct gcacggcttc accacgccag ctgaaatgcc tgacaaattc
     1441 gcgatgtgtg tgcgccaacc ggtgggactc tgtggcttga tcagcccctg gaattttccg
     1501 atggcgatcc cgtcgtggaa actcattcct gctctggtct gtggcaacac agtcgtcatc
     1561 aagcccgccg aagacacacc gctctcaact tacaacctcg tcaaggcatg tgaggaagcg
     1621 ggcattcctc cgggcgtggt caatctcgtt accggttacg gtgatcccgg cgccgccatg
     1681 actaaccatc cctcattgcg tcttatttct ttcactggct caaccgcaac tggccggctg
     1741 gtcgcctcag cttgcgcgga acgcaatgcg atctgttctc tcgagatggg cggcaagaac
     1801 gtcatcatgg tgatggagga tgccgacatc gatctcgcga ttgaaggcgc agtctgggga
     1861 gcgttcggca cctctggtca acggtgcacc gccgcctctc gggttgtggt gcacaagaaa
     1921 gtctacaaga agttttcaca gcagttagtg gaacgcgcga aagcgctccg cgtaggaaat
     1981 ggggctaacc ctaagaccga catgggtccc gttatcaaca acgacgctgt cgacaaaatc
     2041 atgggttacg tcgacatcgg tcagaacgaa gacggcgcga cgctcgcgtg tggcggcaag
     2101 cggctgacgc gcggcgagta cgcgcacggc tacttcatcg aaccgacggt gttcaccgac
     2161 gtcactcccg atatgcgcat cgcgcaggaa gagatttttg ggccagtcac ttcagtgatt
     2221 ccggcgaagt ctctggatga agctgtggag attgcgaatg gtgttcgcta cggtctctca
     2281 gcggcgatct acacgcgcga cgtgaatcgc gccttccacg ccatgaacga aatgtatacc
     2341 ggcatctttt atgtgaatgc ttcgacgatc ggcgccgaag tccatttgcc gtttggcggc
     2401 accaaagcga cgggcaacgg ccaccgcgaa gcgggcactc aggtgttgga tattttctcg
     2461 gaatggaagt ctatctatgt ggactacagc ggaaagttgc agcgggcgca gattgacgag
     2521 gtcaatttat agatgtcggt tttcaggtgt cagctgctag tcaacatagg gatgtcagat
     2581 ttcagatggt agatgctagt cgacgcaa
//
LOCUS       DASUEX010000191         3931 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_491168, whole genome shotgun sequence.
ACCESSION   DASUEX010000191 DASUEX010000000
VERSION     DASUEX010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3931
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491168"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..669
                     /gene="argC"
                     /locus_tag="VFV61_03160"
     CDS             <1..669
                     /gene="argC"
                     /locus_tag="VFV61_03160"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEX4899502.1"
                     /translation="AMDAQPEFVYGLTETNREAIKNARLISNPGCFATATLLGLAPLV
                     ANHWITGRVVVDAKTGSSGSGAKAAANTHHPQRMNSFYAYKPFTHQHVPEIEQELRHV
                     GPWDSELVFMTHSLPVARGIFASIYVETKTELSEAEVHAAFSRFYSDSYFVRIVKNSP
                     DINWVKTTNFCDIGFAVRGRQLVVFSALDNLVKGAAGQAVQNMNLMFGLEETTGLKLI
                     GTNP"
     gene            827..2023
                     /locus_tag="VFV61_03165"
     CDS             827..2023
                     /locus_tag="VFV61_03165"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX4899503.1"
                     /translation="MKQEKKTATSEVAALEERYQVATYKKMPIVAERGEGVWLYATDG
                     QKYLDLYGGHAVAGTGHSHPHMVAALREQADKLLFYSNLVYSEARARAAEKLVTIAPA
                     SLAKVFFCNSGTEANENAMRMARMATGRENVITFSGGFHGRTADAISATFLGKYRELG
                     KPNVPGHLAAEFGDLESVRQLADDSVAAIMLEPIQSMAGVRMAEPEFYAGLRRLCDER
                     GIVLIYDEVQTGVGRTGEWFFAGSEAGAGVVPDIVTLAKALGSGIPVGACLVTEEIAA
                     KIKENDLGTTFGGGMMAMAAVSATLEAIEQDDMLANVKAVEAHLRESLASLPGVVEVA
                     GRGFLLGLRFTQKAAEIHRALLERRIITGTSSDPHVLRLLPPLCLAKPEVDLFVTALR
                     EVCEGC"
     gene            2170..2457
                     /locus_tag="VFV61_03170"
     CDS             2170..2457
                     /locus_tag="VFV61_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899504.1"
                     /translation="MRTRRSIRLSYSWFDIAGLAGVVLIIIAYLLLQLGKLPSTAPSY
                     SLLNAIGAFLVMVSLFFDFNLSAFLMEAFWFLISLFGLLRPWFSRTVVANR"
     gene            2463..3458
                     /locus_tag="VFV61_03175"
     CDS             2463..3458
                     /locus_tag="VFV61_03175"
                     /EC_number="2.1.3.9"
                     /inference="COORDINATES: protein motif:HMM:NF003384.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_function: GO:0043857 - N-acetylornithine
                     carbamoyltransferase activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylornithine carbamoyltransferase"
                     /protein_id="HEX4899505.1"
                     /translation="MKHLLTTGDCSRSELEELLATAHRFKVEGAPKLLADRSIALVFF
                     NPSLRTRASMQVGIYELGGNPVVLEPGGSSWTLEHRTGVTMDGDKTEHVAEFVRVLGR
                     YCSAIGVRTFAALKDWHEERTDPVLQAFAKFAEVPLINLESAMFHPCQALADMMTIRE
                     KLGRERKRVVLTWAWHPKPLPMAVPNSFALAAAQMGHDLVVAHPDGYELDEQLVAHIK
                     QHAAGAGGSLQVTDDIDEAFAGAEVIYAKSWGAKQFYGTPEQDIEWRAQFRERWIVDQ
                     RKMETTNRGIFMHCLPVRRNVIVTDEVIESPASVVIDEAENRLHVQKAILAKLVA"
BASE COUNT          901 a          993 c         1144 g          893 t
ORIGIN      
        1 gccatggacg cgcagccgga atttgtctac ggcctgactg aaactaatcg tgaagcgatc
       61 aagaatgccc gactgatcag caatcccggc tgctttgcta ccgcaaccct gctcgggctg
      121 gcgcccctcg ttgcgaacca ctggatcacc ggtcgcgttg tagttgacgc aaaaaccggc
      181 tcttccggtt caggggccaa agccgcagct aatactcatc atccgcaaag gatgaattcg
      241 ttttatgcct acaagccgtt cacacatcaa cacgtcccgg aaatcgaaca ggaactgcga
      301 cacgtcgggc cgtgggatag cgagctggtt ttcatgacgc actcgttgcc cgtggcgcgc
      361 gggatcttcg cttcaatcta tgttgagaca aaaactgaac tcagtgaagc cgaagttcac
      421 gcggctttta gtcgcttcta tagcgattcg tatttcgtgc gcattgtgaa aaattcaccg
      481 gacatcaact gggtaaagac cactaatttc tgtgacattg gttttgcagt gcgcggccgg
      541 cagttagttg tcttttcggc gctcgacaat ctcgtgaagg gcgcagccgg ccaggcggtg
      601 cagaacatga acctgatgtt tggcttggaa gagaccacgg gattgaaatt gattggcacg
      661 aatccttagt tcagagatca acgctgaggc gcaaaatcgc caagacgcaa agaggagtaa
      721 aaactgataa cgatcaaccc acggaaatgt aagtttcctc ttggaacctt tgcgactcag
      781 cgccttagcg tctctgcgat tggtgtcttg gcggtttaag ttagaaatga aacaagagaa
      841 gaaaacagca acaagcgagg tcgccgcact tgaagaacgc taccaggtag cgacctacaa
      901 gaagatgccg attgtcgccg agcgcggcga aggcgtctgg ttgtatgcca ccgatggcca
      961 gaagtatttg gatctttatg gcggccatgc ggtagccggc accggtcact cgcatccgca
     1021 catggtggcg gcactgcgcg aacaggcgga caagctcttg ttctattcca acctggttta
     1081 ttcggaagca cgcgcacgcg cagctgaaaa gctggtgacg attgcacctg cctcgctggc
     1141 gaaagttttt ttctgtaatt ccggaaccga agcaaacgag aacgcgatgc ggatggcgcg
     1201 catggcaact ggtcgggaaa atgtcatcac tttctccggc ggctttcacg gacgcaccgc
     1261 cgatgcgatc agcgccacct ttctcggaaa gtatcgtgag ctcgggaagc caaacgtgcc
     1321 gggtcatctg gccgccgagt ttggtgacct ggagagtgtg cgacaactag cggacgactc
     1381 agtcgccgcc atcatgttgg aaccgattca gtcgatggct ggtgtccgaa tggctgaacc
     1441 ggagttttat gccggactgc gccggctatg cgacgagcgc ggcatcgttt tgatctatga
     1501 cgaagtgcaa acgggggtcg ggcgtactgg tgagtggttc ttcgctggaa gcgaagccgg
     1561 cgctggtgtt gttcctgaca tcgttacgct cgctaaagca ctcggcagcg gtatcccggt
     1621 aggtgcttgc ctggtcacgg aagagattgc cgcgaagatt aaagagaacg acctgggaac
     1681 aacttttggt ggcggcatga tggcgatggc ggcagtatcc gcaacgctcg aagcgatcga
     1741 acaggacgac atgctcgcaa acgtgaaagc agtggaagcg cacttgcgcg agtcgctggc
     1801 ttcgttaccg ggtgtggtcg aagtcgcagg caggggattt cttttgggtt tgcgctttac
     1861 acagaaggcg gcagagattc atcgggcgtt gcttgagcgc cgaatcataa ccggtacttc
     1921 aagtgatcca catgttttga gactcttgcc gccgttatgc ctagcgaagc ccgaagtcga
     1981 tttgtttgtg acggcgctca gagaagtttg cgaaggctgt tagcgcgggt tgtaacgcag
     2041 cgccagtctt gatggcgctc attttcattc tcaccgcgct tcagcgcggt gtaagacgga
     2101 atgagcgaac accgaaaccg tttaaacggt ttcccactac taacccaatg ctcacctggt
     2161 tgaagcccgg tgagaacgag acggagcatt cgcttgagct acagttggtt cgacatcgcg
     2221 ggactggccg gcgtcgtgct gatcattatt gcctacttgc tcttgcaact cggcaaactg
     2281 cccagcacgg ctccgagtta ctcactgctg aatgccatcg gcgcatttct ggtgatggtg
     2341 tcgttgttct ttgacttcaa cctttcagcg tttctgatgg aagcgttctg gtttctgatc
     2401 agtcttttcg gtctgctgcg accgtggttc tcgagaaccg tggtagcgaa ccgttagcag
     2461 taatgaaaca tcttctaacc accggtgatt gttcgcgaag tgagctggaa gaactgcttg
     2521 ccacggcgca ccgttttaag gttgagggag ctccgaaatt actggctgac agatcaattg
     2581 cgcttgtgtt cttcaatccc agtctgcgga cacgcgcgtc aatgcaagta ggtatctatg
     2641 aacttggtgg taatccggta gtgctcgaac caggcggcag cagttggact ctggagcacc
     2701 ggacgggcgt tacaatggac ggcgacaaaa ctgagcacgt cgctgaattt gtgcgcgttc
     2761 ttggtcgcta ttgttcggcc attggcgtgc gaacttttgc ggcgttgaaa gactggcacg
     2821 aagagcgtac tgatccagtg ttgcaggcat tcgcaaagtt cgcggaagtt ccgcttatca
     2881 atcttgagtc ggccatgttt catccctgcc aagccctcgc ggacatgatg accattcgtg
     2941 aaaagctggg tcgagagcgc aagagagttg tattgacgtg ggcttggcat cccaagccgt
     3001 tgccgatggc tgtgccgaac agttttgctt tggccgcagc acaaatggga cacgatctgg
     3061 tcgtggctca tcccgacggg tatgaactcg atgaacagtt agtcgcgcac atcaaacaac
     3121 acgcggcagg cgccggaggc agtttgcagg taacggatga tatcgacgaa gcgttcgctg
     3181 gtgctgaagt catctatgct aagagctggg gagctaaaca gttctacgga actcccgagc
     3241 aagacatcga gtggcgggcc cagtttcgcg agcgctggat tgtcgatcaa agaaagatgg
     3301 aaaccacgaa ccgtgggatc ttcatgcact gtctgccggt gcgacgaaat gtgatagtta
     3361 cagatgaagt aatcgagtct ccggcttctg tggtaattga cgaagcggag aaccggctgc
     3421 acgtgcagaa ggcgatcctg gcgaaacttg ttgcttaaca gaattgaagc atgcctcaag
     3481 aagtgaggaa ctgttttcga ttcgggtctg ccgcctggtg tgaggcgcgg gccctgcccc
     3541 gccgttcagt tgatcctaga tcctgggcta tgcccttggc ttagctggtt aaagacaatg
     3601 agggcatagc ccaaagtcta ataagttcgt ttccggcggg gcagagccgc cgccttacac
     3661 caaggggcaa agcccgaaac acagtcgggc catgacttcg aggcaaggcc cggatgcaag
     3721 acgatttgtg agaatctttg gcaaacattg aacgcggaag aatatgagga cagccactaa
     3781 cgaagtcggt aatcaattgc gtctcgatct cctgcgcgag gcgttgccct acattcagcg
     3841 tttccagggc cagacttttg ttatcaaact ctccggaaag gccaccgagg atcacaataa
     3901 cctcagctcg ctggcagaag aactggccct c
//
LOCUS       DASUEX010000192         3105 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_491337, whole genome shotgun sequence.
ACCESSION   DASUEX010000192 DASUEX010000000
VERSION     DASUEX010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3105
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491337"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1654
                     /locus_tag="VFV61_03180"
     CDS             <1..1654
                     /locus_tag="VFV61_03180"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899506.1"
                     /translation="EAMTRSLATGEVFVTLNGDWVASGQFVSAGDARAVEEGAGLLAF
                     KRELRELEARSEVLRSELEATEIAAKQARARLVELEDAVVGLNEVIGREERELMTREL
                     TAAGLAQEIERAERHMRVVAEDSARVETERTDVEQRRANALADADAAEAARLAAGETV
                     IKASATLAEARLAADLESEGLALQRATAAAATERRRATTAELRRIESEYADVAARIER
                     HRFEAVEITNRIEELRQSIAYLEQKANAVASEREREEQEISGLLAQLELARQQADSLS
                     SELSEINRRAAEVRNSRGAIEVQRAEGQARLTFVRENCAAELNQSLEELAHEQSLDEQ
                     FDLEAGQARVEELRTRIEGFGAVNMMALDELNESEDRLLFLITQRQDILDGISSTEEA
                     LREIKRRSRERFRHAFEQINKNFGEFFQELFGGGRGEMSLIEADDVLESGIDVIAQPP
                     GKRLQNVLLLSGGEKAMAALALVLGIFHYRPAPFCLLDEVDAPLDEANVGRFTDKVAQ
                     MSAQTQFIVITHNKRTMEMARALYGVTMEEVGVSKLVSVRFE"
     gene            1906..2628
                     /locus_tag="VFV61_03185"
     CDS             1906..2628
                     /locus_tag="VFV61_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899507.1"
                     /translation="MHRLEKLLGTLIIASSAVLAVAQESPNHKQNVRPEKSSSGPVPP
                     SVTPSKSAARFSYEFTQPQFYLRHILIEHDASGQGRITFERLNEEVPIVESLEISPAA
                     LQRISSLWQALRFLDSETNYQSDKQFPHLGTMKLTMIDGERKRTAEFNWSNVQEAADL
                     VKEYRRVADQANFVFDVSVARENQPLNAPKLMEAFESLLKRNGLSDPKQVIPLLKDIS
                     GDERLPLIARNHALRLLAKINK"
     gene            complement(2650..3036)
                     /locus_tag="VFV61_03190"
     CDS             complement(2650..3036)
                     /locus_tag="VFV61_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899508.1"
                     /translation="MWLRLMCTLLIISGASVAAQGKSRWQGCLPADVEANEVIGAESP
                     FSGQPAKKITVTSKLNQIAARCRGKRLVDAKGKQIHFYRLTGCWGNPPENYQEILENQ
                     RQELTRLRRRYRVIEISCNPSGLLIQ"
BASE COUNT          720 a          852 c          887 g          646 t
ORIGIN      
        1 cgaggccatg actcggtcgc tcgcgacagg cgaagtattt gtcactctca atggcgactg
       61 ggtagccagc ggacagtttg ttagcgccgg agatgcgcgc gccgtggaag agggtgcggg
      121 cctcctcgca ttcaaacgag agctgcgaga gctggaggcc cgttccgagg tcttgcgttc
      181 agaattggaa gcgaccgaaa tcgcggcaaa gcaagcgcgc gctcgtctag tcgaattgga
      241 agatgcagta gttggcctta acgaagtgat cggacgtgag gagcgcgaac tcatgacgcg
      301 cgaactgacg gccgctggct tggcacagga gatcgaacgc gcagagcgtc acatgcgtgt
      361 agtcgctgag gattcagcgc gtgtggaaac ggagcgcact gacgtcgagc aacgccgcgc
      421 caatgccttg gccgatgccg atgccgcgga ggctgcgcgt ctcgctgcgg gtgaaacggt
      481 gatcaaggct tcagcgaccc ttgccgaagc gcgtctcgcc gcggacttgg agagtgaagg
      541 tcttgccttg cagcgcgcca cagccgcggc agctactgag cgaagacgtg ctactacggc
      601 agaattacga cgcatcgaga gtgagtatgc cgatgttgcc gcgcgtatcg aacggcaccg
      661 atttgaggcc gttgagatta ccaaccggat tgaagaactg cggcagtcaa ttgcatatct
      721 ggagcaaaag gccaacgccg ttgccagtga gcgggagaga gaagaacagg aaatctccgg
      781 attgttggca caactggaac tggcgcgtca gcaggctgat tcactctcca gcgagctttc
      841 ggaaattaac cggcgcgccg ccgaagtgcg caactcacgc ggcgcgatcg aagtgcaacg
      901 cgccgaaggt caggcccgcc tcacctttgt gcgtgagaac tgcgcggccg aattgaatca
      961 atcccttgaa gagctggcgc acgaacagag tctggacgag cagtttgatc tggaagccgg
     1021 tcaggcccgg gtagaggagc tgcggacacg cattgaaggc tttggcgccg tgaatatgat
     1081 ggcgcttgat gagttgaacg aatcggaaga ccgcttgttg ttcctcatca cgcaacgcca
     1141 ggacattctt gatggcatca gttctacgga agaggcgttg cgcgaaatca aacgccgctc
     1201 gcgcgaacgt ttccggcatg ctttcgaaca gatcaacaag aacttcggcg agttcttcca
     1261 ggagcttttt ggcggcggtc gcggcgagat gagtttgatc gaagccgacg acgtgttgga
     1321 atccggcatc gatgtgattg cccaaccgcc gggcaagcga ctgcaaaatg ttttgttact
     1381 ttccggcggc gaaaaagcaa tggctgctct ggcgctggtt ctgggaatct ttcattaccg
     1441 gcccgcgccc ttctgcctgc ttgatgaagt tgatgcgccg cttgatgagg ccaacgtcgg
     1501 cagatttacc gacaaggtgg cgcaaatgtc agcccaaacc cagttcatcg tcatcacgca
     1561 caacaagcga acgatggaga tggcgcgggc tctctacgga gtaacaatgg aagaggttgg
     1621 cgtgtctaaa ctggtttccg taagatttga gtagggaaaa cgggagcgac tttcatggcg
     1681 ggccctgccc gcctttttca ttgccaattg ccaattgccg attgccaatt ggaggctcag
     1741 aatcacgttt gattgaatcg caaggacttc taatcaggtt tccaattgga aatcggcaat
     1801 cgtcaatcgg caattagctg gcggttcatg cttgcaacct cccctcaact gcgtggcgca
     1861 aaccttttgc cgtaaagtac catcacaacc gaccggtgaa gacgtatgca tagacttgag
     1921 aaactcctgg gaaccctcat tattgcaagc agtgcggtgc tggcagttgc gcaggagtcg
     1981 ccgaaccaca aacaaaatgt tcggccggag aagtcgtcct ccggcccggt cccgccgtcg
     2041 gtgacgccga gcaagagtgc ggcacgtttc agctacgagt tcacccagcc gcagttttat
     2101 ctgcgtcaca tcctgattga gcacgatgct tccggccagg gcagaatcac tttcgaacgc
     2161 cttaacgaag aggttcccat cgtcgagagt cttgaaatat cgcccgcggc tttgcagcga
     2221 atctccagcc tctggcaagc gctcagattt ttggattcag agacaaacta ccagtccgat
     2281 aaacagtttc ctcacttggg tacgatgaaa ctgacgatga tcgatggcga gcgaaaacga
     2341 acggcagagt tcaactggtc aaatgttcaa gaggcggccg atctggtgaa ggagtaccga
     2401 cgagtcgctg accaggcaaa ttttgttttt gacgtctctg tggcccgcga gaaccaacct
     2461 ctcaatgcac ccaagttaat ggaggcattc gaatcgctcc tgaaacgaaa cgggctatca
     2521 gatcccaaac aggtaatccc attactgaag gacatcagcg gcgacgaacg attgcctcta
     2581 attgcccgga accacgcatt gcgattgctt gcgaagatca acaaatagtc caccgctccc
     2641 agaccctctt cactggatca acagcccgct gggattgcag ctaatctcaa tgacacggta
     2701 tcgccggcgc aaccgtgtca actcctgtcg ttgattctcc aggatctcct gatagttctc
     2761 gggcggattt ccccagcaac cagtcagccg gtagaaatgg atttgctttc ctttagcgtc
     2821 caccaggcgt ttgccacgac agcgcgcagc gatttggttc aacttggacg ttacagtgat
     2881 cttttttgcg ggctgacccg agaacggaga ctccgcgccg atcacttcat tagcctcgac
     2941 gtcggcgggc aaacatccct gccaccgcga cttcccctgc gccgccacac tcgcgccgct
     3001 aataataagc agggtgcaca tgagtctcag ccacatcgtt tttgttcctc aacgctaacc
     3061 cattagattt gcggaaaccc agcctccgcc gcgcagttag atgcc
//
LOCUS       DASUEX010000193         2413 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_955635, whole genome shotgun sequence.
ACCESSION   DASUEX010000193 DASUEX010000000
VERSION     DASUEX010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_955635"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            583..831
                     /locus_tag="VFV61_03195"
     CDS             583..831
                     /locus_tag="VFV61_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899509.1"
                     /translation="MNLDKGLPLAERLQRLDHIQARRFSKLAGTVLEIATEGILRHLA
                     ACDRMDVNPDISAVREIIDDALNGRRVYAEAAEITRAA"
     gene            948..1023
                     /locus_tag="VFV61_03200"
     tRNA            948..1023
                     /locus_tag="VFV61_03200"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:981..983,aa:Arg,seq:tct)
     gene            1109..1185
                     /locus_tag="VFV61_03205"
     tRNA            1109..1185
                     /locus_tag="VFV61_03205"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1143..1145,aa:His,seq:gtg)
     gene            1210..1476
                     /locus_tag="VFV61_03210"
     CDS             1210..1476
                     /locus_tag="VFV61_03210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899510.1"
                     /translation="MPEAQRTATHSQTFVADAETFEFETVEQENGQATVIRFSLDDPR
                     YHAGDVVLVLSGTDIHFHGMIGKIADGTAIATDRRGSLLPATTQ"
     gene            complement(1480..>2413)
                     /locus_tag="VFV61_03215"
     CDS             complement(1480..>2413)
                     /locus_tag="VFV61_03215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899511.1"
                     /translation="MPRFPKLFAPLLLLVLVLASQLSAGQGGSRRSRGAPRPVTIPVT
                     IKVNKPETEIRVVDLMIREDGEVQSLLSIRRPADNPVTLAVLLQDDLVSSIASEARGV
                     ANFIRNLPGGSRVMVGYIRNGSLQVRRRFTTDLDRAAASVRPPLGSTSASPFNPYVEI
                     IEALRRFDSQPLGRRAIVVVSDGLDVSRGLDSSSAADSIDLARAIREAQRRGVAIYSI
                     FAPPALSSNQFLIANGQSSLQRLSDETGGRAFFQGTSAPVSFQPFLRDISLTLERQIA
                     LTYLSTHPNKGFHRLEVKPLDRDVEVRHPAGYTR"
BASE COUNT          533 a          661 c          706 g          513 t
ORIGIN      
        1 ctgagcgacc tggacaccgt cgtcggcgcc atcctcgacg agctcgagaa catccggtcg
       61 gtcctgctcc acggcgagcc cgagcagcag cgggaactgg tgcggcgctt cctggtgggg
      121 atgagacgat tcaaatcccc gcgaaaacca cgctgaaatt ccgcgtcggc aaagcgatga
      181 aggattcggt tctgccacgt aagtaacgcg gttttcgccg aatcttagta tcgcggcgag
      241 cagattcaca ccggcgtagc gtccctcatc gggcgcttcg ccggtgtttt ctttaccgca
      301 gccccttgcg ggccgagttt agcgatcttc gccgtgacaa agaatttaca aatttccgcg
      361 taaaagatct tcccgtcacc gcctcgtcgt gttatagttt cgggtttcaa agtcacagtt
      421 taggctttct gtggcccctc tttttgggtt cgactgggtt cttactgtac aaaccaaccg
      481 aaactgcaag gactttgcat cctgggaagt ttcggcggca tctatagttt agacaaatca
      541 acgcgctgat tttacctgtt tagaaggaga gaatatttaa tcgtgaatct tgacaagggc
      601 ctgccgctgg ctgagcggct tcagagacta gatcatattc aggcacggcg tttttcgaag
      661 ctggccggca cggtgttgga gattgcaact gagggcatcc tgcgccatct ggcggcgtgc
      721 gatcggatgg atgtgaatcc ggatatttct gcggtgcgag agattatcga cgacgcgctt
      781 aacggccggc gtgtgtacgc tgaggcggct gagattacgc gcgcagctta accgcaacac
      841 tgcaattcaa actcgaaccg aggctgtccc ataggggcgg cctctcgctt tgttcagtcg
      901 gtttattcgc gagtaacgtg gcgtgctaac atctcgacgc aacttgggct cccgtagctc
      961 agcggataga gcatctgcct tctaagcaga gggccgcagg ttcgagtcct gccgggagca
     1021 ccattaggag gatatccacc ctcgcttgtc gaaaccacgc gagtgttgta aacaagaggt
     1081 tgatgcaatt ttaagccgat catttgcggc ggctatagtt cagttggtta gaacgcctga
     1141 ttgtggatca ggaggtcgtg ggttcgagcc ccactagccg ccccactacg aattcccacg
     1201 ggagattaaa tgccagaagc tcaaagaaca gctactcatt cgcagacatt tgtcgcggat
     1261 gccgagactt tcgaattcga aactgtcgag caggaaaatg gacaggccac tgtgattagg
     1321 ttcagtctgg acgatccacg ataccatgcg ggtgacgtag ttctggtgtt atccggcacc
     1381 gacattcatt ttcacggaat gattgggaag attgcggacg ggacggcaat tgccacggat
     1441 cgtcggggct cgttgctgcc ggccacaact cagtaacgct caacgcgtgt agccggcagg
     1501 gtgacgaacc tctacgtccc ggtcgagcgg cttcacctcc agccgatgaa aacccttgtt
     1561 cggatgcgtc gacagatagg tcagcgcaat ctgccgctcg agtgttaaag aaatgtctct
     1621 caagaagggt tgaaagctga cgggcgcgga ggtgccctgg aagaaagctc ggccgccagt
     1681 ttcatctgaa agtctctgta acgaactttg gccgttcgcg atcaggaact ggttactcga
     1741 aagcgcgggc ggcgcgaaga tggagtaaat cgcgacaccg cgacgttggg cttcccggat
     1801 cgccctggca aggtcgatgc tgtcggccgc agaagaactg tcgagcccgc gcgaaacatc
     1861 caggccatcg gaaactacca ctatcgctcg ccgtccgagc ggctgggaat caaagcgacg
     1921 caaggcctcg attatctcaa cgtatgggtt gaaggggctg gcactcgtgc tgccgagagg
     1981 cggtcgcacg ctggccgccg ccctatccag atcagtggtg aacctgcgcc gcacttgtaa
     2041 gctgccattg cgaatgtagc caaccatcac gcgcgatccg ccgggcaagt tccggatgaa
     2101 attggcgacg ccccgcgctt cgcttgcaat cgacgacacc aaatcgtcct gtagcaacac
     2161 tgccagcgtc accggattat cggcgggccg gcggatggaa agcaggctct ggacctcgcc
     2221 gtcttcgcgg atcatcagat cgacaacacg aatctcagtc tcgggcttat taactttgat
     2281 agttacaggg atcgtgaccg gacgtggcgc gccacgcgac cgacgcgaac ctccctgacc
     2341 ggcggagagc tgcgacgcca gcacgagaac caacagcagc agaggagcga atagttttgg
     2401 aaaccggggc atg
//
LOCUS       DASUEX010000194         2921 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_955668, whole genome shotgun sequence.
ACCESSION   DASUEX010000194 DASUEX010000000
VERSION     DASUEX010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2921
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_955668"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1157
                     /locus_tag="VFV61_03220"
     CDS             <1..1157
                     /locus_tag="VFV61_03220"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX4899512.1"
                     /translation="TAKPLAAAVALILLLSAGVIAQGEDANKLELVRAHYETAQALER
                     AGDWVKATEEWSALLKLAPDDARAWTNLGVALNRQSKSSEALAAWSRAIEIDPKLAGA
                     HFNVGLTLVKDSKYVEAVAPLRRALALEPTNEAARRTLALALIGSERFREATREIAQL
                     LVRAPRDTGLLEMAAQNFMRQGRFQEAAVVLRRRVDIDPASSSLWALYGDALDGSART
                     PEATAAYQKALELDPESTQIRYGLGYLYWKLYRYEEAERELVEVLRRDSDNASAAFTL
                     GDLYLTKGMANQAQPFLEKAAAAYPNEFDTRFALGRALVLQGDLTRGIVELRAAVLLN
                     GSIADGHYQLGRALMRTGAKDEARRELDRARELQEQRRSAEAERFLKKLP"
     gene            complement(1182..2390)
                     /gene="nagA"
                     /locus_tag="VFV61_03225"
     CDS             complement(1182..2390)
                     /gene="nagA"
                     /locus_tag="VFV61_03225"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEX4899513.1"
                     /translation="MKDGEFKSMLIRGARIVLPEGVTSGKSLLVEAGRIVRIDEESNR
                     LRAEISKDFTGLTLYPGFVDMHIHGADGVDTMQATAADLRQVAKFLVKAGVTAWMPTL
                     VPAPDIDYQSAVTAIEEVMLEQPQRQTDARIVGVHYEGPFVNSVQCGALRSAYFKSFS
                     ESTDIDGLPTPKDCGAIRMMTLAPEIEGGIRLVRELKERGWISALGHTRAEVDVMDEA
                     FAAGARHMTHFMNAMPPLHHRAPGPVAWGLMKDEVTCDIIADGIHLDPLMLRLLLRKK
                     GADRLALISDAIAAAGKGDGQYMIWGETIQVKGGRTRNANGNIAGSVITMADAVRMML
                     SLGASESEVALMASTNPARILGIDNECGSIAEGMRADLAGLDDKGKVRLTMVGGAIVF
                     DDESGGSTNQ"
     gene            complement(2473..>2921)
                     /locus_tag="VFV61_03230"
     CDS             complement(2473..>2921)
                     /locus_tag="VFV61_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899514.1"
                     /translation="ITDITSGQPVARVGTASAMPAGGALTGFYPNAIRDPNGGLAGTV
                     DASGLPYIFDPTALAAPANGTYGNLPRAFARLFGRNQTNLTLTNNVYFDAERNVRLQL
                     RAESFNVFNHTQFTGVGVNLTDLGTLGRPTGTRLPREFQFGAKLSF"
BASE COUNT          644 a          824 c          828 g          625 t
ORIGIN      
        1 tgaccgctaa gccactagcc gcagctgttg cgttgattct tcttctgagt gccggagtga
       61 ttgcgcaagg ggaagacgct aataaattgg agttggtgcg tgcgcactat gaaacagcgc
      121 aagcactcga gcgcgcgggc gactgggtta aggccaccga ggagtggtcc gctctgctta
      181 aacttgcacc ggatgatgcg cgcgcttgga ccaacctcgg ggtggcgctc aatcgacaaa
      241 gcaagagctc tgaagctctt gcggcctgga gccgcgctat cgaaatcgat cccaagcttg
      301 ctggcgcgca ctttaacgta ggtttgacac ttgtaaagga cagcaaatac gtagaagcag
      361 ttgcaccgct tcgtcgcgcg ctggctctcg agccgacaaa tgaggcagcc cgccggaccc
      421 tagcgctggc attgatcggt agcgaacggt ttcgcgaagc cacgcgtgag attgcgcagc
      481 tcctggttcg cgcaccgcgt gatactggcc ttctagaaat ggcggcccag aacttcatgc
      541 gtcaggggcg ttttcaggag gccgccgtag ttctgcgaag gcgagttgat attgatcccg
      601 cgtccagctc gctctgggcc ctgtatggag acgcgctcga tggctcggca cgaactccgg
      661 aagcaactgc tgcttatcag aaagcattag agcttgatcc agaatcgacg cagattcgtt
      721 atggcctggg ctatctttac tggaaacttt atcgctatga agaagccgag cgcgaactag
      781 tagaagtgct gcgccgagat tcagataatg cgagcgctgc tttcacgttg ggcgatctgt
      841 atctgactaa agggatggca aaccaggctc aaccgtttct tgaaaaagca gccgctgcat
      901 atccgaatga gtttgatacc aggtttgcgc tgggcagagc actggtgctt cagggggacc
      961 tgacacgcgg gattgtcgag cttagggcgg cggtcctact taatggctca atcgccgatg
     1021 gtcattacca actcgggcga gcgctaatgc ggacgggggc gaaagacgag gctagacgcg
     1081 agctggatag agcccgagag ttacaagagc aacggcgctc cgcggaagct gaacgcttcc
     1141 tcaaaaaact gccctaggat taggttcggg taccgatctt tctactgatt agtgcttcct
     1201 ccagattcgt cgtcgaatac gattgccccg cccaccatcg tcagccgcac ctttccctta
     1261 tcgtcgaggc cagcaagatc cgcacgcata ccttctgcga tcgagccgca ctcgttgtca
     1321 atgccaagga tccgtgctgg attcgtcgac gccatcaatg caacctcgct ttcagatgct
     1381 ccgagggaca gcatcattct cacagcatcg gccatcgtga tcactgaccc ggcgatgttg
     1441 ccgtttgcgt ttcgcgttcg tccgcccttc acctggattg tttctcccca aatcatgtat
     1501 tgaccgtcgc ctttgccggc agcggctatc gcgtcgctaa tcaaggccaa acgatccgcc
     1561 cctttctttc gcaacagcag gcgaagcatc aagggatcca gatgaatgcc gtcggcgata
     1621 atgtcgcagg tcacctcatc tttcatgagg ccccaagcaa ccggaccggg cgcccggtga
     1681 tgtaaaggtg gcatggcgtt cataaaatga gtcatgtgac gtgcgcctgc ggcaaaggcc
     1741 tcgtccatca cgtcgacctc agcccgagta tgtcccagag ctgaaatcca tccccgttcc
     1801 ttaagctcgc gcaccaaccg gatgccaccc tcgatctcgg gcgccaacgt catcatgcgg
     1861 atagcgccgc agtctttggg tgtaggcagc ccatcgatgt cggtcgactc tgaaaagctt
     1921 ttgaagtatg cagatcgaag cgcaccacat tgaaccgaat tcacaaatgg cccctcgtaa
     1981 tgcactccca cgattcgcgc atcggtctga cgctgaggtt gctccagcat gacttcttca
     2041 atcgcagtaa cagcgctttg gtaatcgatg tctggcgcag gcacgagtgt tggcatccag
     2101 gcagtcacac cggctttgac taaaaactta gcaacctgcc gaaggtccgc ggctgttgct
     2161 tgcatggtgt ccactccgtc agcgccatga atgtgcatat ccacgaaacc gggataaaga
     2221 gtgaggccgg tgaagtcttt ggatatctcc gcacgaagtc gattggattc ctcgtcgatg
     2281 cgaacaatgc gaccagcctc aactaacagc gattttccag aagtgacacc ctccggcagt
     2341 acgatgcggg cgccgcgaat gagcattgat ttgaattcgc cgtctttcat gatgaccctc
     2401 gcgcggatgc taatgtgccc gcgcagatca aagaactacg cgggcacatt agacctattt
     2461 aaacccaaag cgctagaaag aaagctttgc gccgaactgg aactctcggg gcaggcgtgt
     2521 acctgtgggc cttcccagcg tacctaggtc agtcaggttc actccaacgc cagtgaactg
     2581 agtgtggttg aagacgttga atgattcggc gcgcagctgg aggcgaacgt tccgttccgc
     2641 gtcgaagtaa acgttgttcg tcagagtcag gttggtctgg ttgcgcccga acaagcgagc
     2701 gaatgcacgc ggcaggttcc cgtaggtgcc gtttgccggc gccgccaaag cggtgggatc
     2761 gaagatatac ggaaggcccg aagcgtcgac agttccggcg agcccaccgt tcggatcgcg
     2821 aatcgcattg ggatagaacc cagtcagcgc gcctcccgct ggcatggctg aggccgtgcc
     2881 aactctagca accggttgac ctgaagtaat gtccgtgatg c
//
LOCUS       DASUEX010000195         4181 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_254083, whole genome shotgun sequence.
ACCESSION   DASUEX010000195 DASUEX010000000
VERSION     DASUEX010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4181
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254083"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1563)
                     /locus_tag="VFV61_03235"
     CDS             complement(<1..1563)
                     /locus_tag="VFV61_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899515.1"
                     /translation="MFSFHRFLVCLIFLLALTPSGVAQSRLTAEQWREDLRYFAANMQ
                     KTHRNLFHSMTREQFESAVKRLDERIPTMADHEIIVELMRIVGMVGDGHTGVRINQAF
                     TSAYPVRLYFFKDGLFVQGAAPEYREAVGGRVLRIGSSSVDQAIRAVSELAWHDNEMG
                     LKATTPRLLVIPEVLHALRIADNLQSVQLEVEKDGRRISLELKPTGQLRQLIDQNPST
                     WIDARNSAAQTPLWLRDPHNNFWFQHLTGAEAACAEGCEKGIAYVQFNAVQDRPPAGA
                     KPGETVEAFFTKVFDYVEANKIDKFVLDMRLNGGGNNYLNLPLTIGMIKSRVNKRGNL
                     FVIIGRETFSAAQNTVNELEKYTNAIFVGEPTAATPNHFGDARNVVLPNSKISIRAST
                     LWWQDMDPRDTRKWTAPQIAAELTSTDYKASIDPAMAAILKFVPGNGLPQQLTSALKA
                     GGYAAASKAYRSFREDPAHVYLEPEADVNRLGYELLRDQRLDEAIEIFKLNVEAFPRS
                     ANTYDSLAEAYLA"
     gene            complement(1691..2317)
                     /locus_tag="VFV61_03240"
     CDS             complement(1691..2317)
                     /locus_tag="VFV61_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899516.1"
                     /translation="MKRCPQCEFIYEDDQIRCDMDGNDLVFDHPTPSVTSQDPLPQAK
                     RNSSRRALLSFCAIVFGVLVFAIGYASLERAMTVSSEPASLPQVSANEPARQEELAPA
                     PAKEAIVPGAEDPTVTAASNTAKTTRLAVRDPSHNLTSKDGVEPLERNSLGTRGVILG
                     SVPQQNRIESSRHQPPMVRANTPPPPAKKDSKVVSIVKKTGRFFKKAF"
     gene            complement(2461..>4181)
                     /locus_tag="VFV61_03245"
     CDS             complement(2461..>4181)
                     /locus_tag="VFV61_03245"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX4899517.1"
                     /translation="LLTPHYVCAQQRAQINASHNRPRLVLLIVADQFRYDYLERFGDL
                     FGEKGLKRLMREGASWVEANYDHFPTYTAPGHATLLTGAYPAANGIVGNDWPDRETGK
                     KVSSVSDETTVVLGGAQPETGASPRRLLASTLGDELRLSTNDRSKVIAISLKDRAAIL
                     PAGRHANAAYWFSPKSGSMVSSDYYFKELPAWVTRFNSQRPADKFFQAKWERLLPLEE
                     YQKHAGADAPPWEDIGKVVGATNSFPHIISGGASKTGPAFYDEVQFSPFGNEIELSFA
                     KQTIEEEGLGRDEDPDVLSISFDANDYVGHRYGPYSQEVMDITLRFDQQVAQLLDFID
                     SRIGLSNTIVVFTADHGVAPGPKHVIAAGLAADRVDYAEIQRRVRAAISARYNPQAKS
                     PDPTADYLYRYDDAGKATDAIINNNIYFNLEALKRDRVEVEDIERVAGEAALTVPGVA
                     RYFTRGQLLRGGALASSASGSSVDPIARRVQHGFFARRSGNVIFVTEPFTYSSTAVNA
                     THGSPYAYDTHVPLIIRGDGIAGGRYFQAATPADIAPTLAAVLRIQAPSNVTGRILLE
                     ALQGKD"
BASE COUNT          900 a         1119 c         1175 g          987 t
ORIGIN      
        1 tgccaggtat gcctcagcca ggctgtcgta agtatttgcc gaccgcggaa acgcctcgac
       61 attcaatttg aaaatttcga tcgcctcatc aagtcgctgg tcccgcaaca gctcgtatcc
      121 aagccgattc acatcggcct cgggttccag atagacatgc gccggatcct cacggaacga
      181 acggtaagct ttgcttgcgg cggcgtatcc acctgctttt agcgcgctgg ttagttgctg
      241 cgggagacca ttcccgggaa caaacttcag aatcgctgcc attgccggat cgatgctggc
      301 cttgtaatca gtcgaggtca gctcagctgc tatctgcggc gctgtccact tgcgcgtgtc
      361 acgcggatcc atgtcttgcc accaaagagt tgaggcacgg atcgagatct tgctgttggg
      421 gaggactaca tttctcgcat cgccgaagtg gtttggagtt gccgccgtgg gctcaccaac
      481 aaaaatggcg ttcgtgtatt tctccagctc gttgacggtg ttctgcgcag cggaaaaggt
      541 ttcgcgccca atgatgacaa agagattgcc ccgtttgttg actcgcgact tgatcattcc
      601 gattgtgagc ggcagattga gataattgtt gccgccaccg ttaagacgca tgtcgagcac
      661 aaatttatcg atcttgttcg cctccacata atcaaagact tttgtgaaaa atgcttccac
      721 tgtttcccca ggctttgctc cagctggtgg tcgatcttga acggcgttga actgaacgta
      781 agcaattcct ttttcgcacc cctcagcaca ggcagcttct gctcccgtta ggtgttgaaa
      841 ccagaagttg ttatgcgggt cgcgcagcca gaggggagtc tgcgctgcgg agttcctcgc
      901 atcgatccag gtagacggat tctgatcaat taattgccgc agctgccccg tcggtttcaa
      961 ttcaagactg atccgacgtc catctttttc gacctccaac tggacacttt gcaagttgtc
     1021 agcgatgcgc aaagcatgga gcacctcggg aattaccaac aggcgtggcg tcgtcgcttt
     1081 aagccccatt tcgttgtcgt gccaggctaa ttcgcttacc gcgcgtattg cctgatcgac
     1141 gctactactt ccgatccgca gcactcgtcc acccactgcc tcccgatact cgggagctgc
     1201 cccttgaacg aagagaccgt ccttaaaaaa gtaaagacgg acgggataag cggacgtgaa
     1261 cgcctgatta atccgaacgc cggtgtgacc gtcgccgacc atccctacaa tccgcatcaa
     1321 ctcgacgatg atctcgtgat cggccatcgt gggaatgcgt tcatcaagcc gcttcacggc
     1381 cgactcaaat tgttcacgag tcatgctgtg aaaaagattg cggtgagtct tctgcatgtt
     1441 tgcggcgaag tagcgcaggt cttctcgcca ttgctccgcc gttaatcgcg actgggcgac
     1501 gccgctcggc gttagagcca gcaggaagat caggcagacg aggaatcggt ggaaagaaaa
     1561 catagcgagc ctccagttaa ggtttgtctc agataggcgg cgccttcacc gcaggaatgt
     1621 gagcggacag actaatccca gggtgtctac ctttaaaaac accacggctg agacctttgt
     1681 gtcaggaaac ctagaaggct ttcttaaaaa agcgccctgt cttcttcaca atcgaaacga
     1741 ctttcgaatc ttttttggca gggggaggag gagtatttgc tcgcaccatt gggggttggt
     1801 gtcgcgacga ctcgatacga ttctgctgcg gaacacttcc gagaatcacg cctcgggttc
     1861 cgagggagtt gcgttccagt ggttccacac cgtcttttga cgtcaaattg tgcgatggat
     1921 cgcgcacagc caatcgcgta gtcttcgcag tgttgctggc agctgtcacg gttggatctt
     1981 cggcgcctgg cacaatcgct tccttagctg gagcgggggc gagctcctcc tgcctcgcgg
     2041 gttcgttggc agagacttgc ggcagcgatg ctggttctga actgaccgtc atggctcgtt
     2101 caagtgaggc atagccgatg gcgaaaacca gtacgccaaa tacgattgcg cagaacgaga
     2161 gtaacgcgcg tcgcgagctg tttcgcttgg cttgaggtaa aggatcttgc gaagtcacag
     2221 aaggcgtagg gtgatcaaaa acgaggtcgt ttccatccat gtcgcaacgg atctggtcgt
     2281 cctcatagat aaattcacac tgcgggcacc gtttcatttt ctgcctcccg gataggttgt
     2341 ctttggggcg cggtagttta aatcggggtt aggggtgtga gagttaccgc gggttgagaa
     2401 tataccgttt ggctggggcg agacaacaaa aaagattttg ggcgctaagt cgaaccgttt
     2461 ctagtctttc ccctggagtg cttccagcag aattcgccca gtaacattgc tgggcgcctg
     2521 tattctaaga actgcggcga gggtgggggc gatatcggca ggggtcgccg cctgaaagta
     2581 cctgccaccg gcgatcccgt cgccgcgaat aattaatgga acatgcgtgt cgtaagcata
     2641 aggggagccg tgggtggcgt ttactgcagt actggaatag gtaaatggct ccgtgacaaa
     2701 aatcacgttg ccgctgcgcc gcgcaaagaa gccgtgttgc actcgccggg cgatcggatc
     2761 aactgaagat ccacttgcgc tgctggcaag agcgccccct cgcaggagct gtccgcgcgt
     2821 gaaatagcga gcaaccccgg gaactgtcag cgcggcttca ccagcaacac gttcaatatc
     2881 ttccacttca acgcggtcgc gcttcagcgc ctccagattg aaatagatgt tgttgttgat
     2941 gatggcgtcg gtcgctttgc ctgcgtcatc atagcgatac aaataatcgg cagtcggatc
     3001 cggagacttc gcctgaggat tgtatcgggc actgatggcg gcgcgtactc gtcgctggat
     3061 ctctgcatag tcgacgcgat cggcggcgag tccggcagcg attacatgtt tcggcccggg
     3121 cgccacgcca tgatcagcgg tgaacacaac gattgtgtta ctgagtccga tacgtgaatc
     3181 gataaagtcc aacaactgcg cgacctgctg atcaaatcga agcgttatgt ccatcacctc
     3241 ctggctgtac ggcccataac ggtggccgac gtagtcgttg gcatcgaagc tgatgcttag
     3301 cacatccggg tcttcatctc ggccgaggcc ctcctcttca atagtctgct tggcaaagga
     3361 aagttcgatt tcatttccaa agggagagaa ctggacctcg tcataaaatg ccggccccgt
     3421 tttggaagct ccaccgctaa taatgtgcgg aaacgagttt gttgcgccta caactttgcc
     3481 gatgtcctcc caaggtggag catctgcacc ggcgtgcttt tgatattcct ccagcggcag
     3541 tagccgttcc cattttgctt ggaagaactt atcggccggg cgttgggaat tgaacctcgt
     3601 gacccaggct ggcaattcct taaagtaata atctgaggaa accattgaac ctgatttcgg
     3661 gctgaaccag taggcggcgt ttgcatggcg tcccgcgggc aagatcgccg cccgatcctt
     3721 gagtgagatc gcaatcacct tcgaacgatc gttggtactg agccgcagtt catcgccgag
     3781 cgtagacgct aatagtcgcc gcggactcgc cccagtctct ggttgggcgc cgccaagcac
     3841 gacggtagtt tcatctgaga cgctgctgac ttttttcccg gtttcgcgat cgggccagtc
     3901 attaccgaca atgccgttgg cagcgggata agctcccgtc aagagagtcg cgtgtcccgg
     3961 ggcggtgtag gtcggaaaat gatcgtaatt cgcctcgacc catgacgctc cttcgcgcat
     4021 tagtcgtttc agaccttttt cgccgaagag atcgccgaag cgctccaggt agtcgtaacg
     4081 aaattgatcg gcaacgatca agagcacgag gcgaggtcga ttgtgcgaag cgttaatttg
     4141 ggcgcgttgt tgcgcgcaca cgtagtgcgg tgttaagagg c
//
LOCUS       DASUEX010000196         6239 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_956288, whole genome shotgun sequence.
ACCESSION   DASUEX010000196 DASUEX010000000
VERSION     DASUEX010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6239
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_956288"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(214..603)
                     /locus_tag="VFV61_03250"
     CDS             complement(214..603)
                     /locus_tag="VFV61_03250"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX4899518.1"
                     /translation="MNSLKKAPLGNHSRYSRRRNPVSGCPLTAALAAVGGKWKLIIVY
                     WLAESPKHFAALRKLMPDISQKVLTQQLRELADDDVVQRDPKGAVPAPVEYSLTDYGR
                     TLLPLLESIRGWGNAHIKRMTAPPDAS"
     gene            670..1005
                     /locus_tag="VFV61_03255"
     CDS             670..1005
                     /locus_tag="VFV61_03255"
                     /inference="COORDINATES: protein motif:HMM:NF019590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HEX4899519.1"
                     /translation="MIVEVRSYRIKPGRRAEFIHLFETRAVAALRAQGMKVVGPFLDL
                     ENPNKFVWLRSFPSLDERERMRNAFYEGDLWKNELEAIAMPMLESYDVTLCETSPGCV
                     FDLPQTINQ"
     gene            1035..1610
                     /locus_tag="VFV61_03260"
     CDS             1035..1610
                     /locus_tag="VFV61_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899520.1"
                     /translation="MNRGRHFPLALLLLTSLCLTSPSVNAQPESAATPQRDGQHDFDF
                     EFGGWKAHISRLLKPLTGSKTWMEYDGTSVVRKVWDGRANLGELNVSGPQGHIQGLSL
                     RTYNPQSHQWHIGWVNSADGTMGPPMIGGFKNGRGEFYNQESFNGRAIFVRFIFSDIT
                     ANSFQIEQAFSDDGGKTWEANWIAKFSRVKG"
     gene            complement(1639..2454)
                     /locus_tag="VFV61_03265"
     CDS             complement(1639..2454)
                     /locus_tag="VFV61_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899521.1"
                     /translation="MKFQILLLTGVVISLTLTSSLSSAPTQQQAAAPATTQSAELAEA
                     EELSTQVITLYHERKYDKALSLAKRVLMLREKALGPEHFLVADALANVAELQYVRGLR
                     NEALRTYRRYLITYDKNRDVDARDTRKVMISALTRNICLLLSSLRTEYKDFEEAFALQ
                     KRLYKLENGFDFDEAANHPIDDLRRGGLLINKRISGPSPVTPRSRHVFGSLIMKIQVD
                     EQGRVIAAKTICGDQFVIEASEEAMLKTTYKPTTVDGKPVPVTALALYYFQAY"
     gene            complement(2454..3740)
                     /locus_tag="VFV61_03270"
     CDS             complement(2454..3740)
                     /locus_tag="VFV61_03270"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX4899522.1"
                     /translation="MPRVKLSVLLVLLGFFAQAVSAPQTNFTRQQYEEDFDYLWQSFS
                     DDYAYFDQKQTDWQRVREIYRPQVSVVRTRDDFVGLLESVLEELYDFHTHLNTNTPSS
                     SRLVPSGADMWAEWIQDRAIITEVRPESLAEKARIKVGMQIVSINGVAVAEAVSRRTG
                     KSLRSVDKPARDWALRILLAGRHNEKRSVEVMTNGDRSVLSIDNESAEAAAAQKFLLE
                     RSKLQNDLGYIRINNSLGDTELIKQFDLALASLRNTRGLILDLRDTPSGGNTTVARGI
                     MSRFISRDMPYQKHSLPAEEHRYGIRRSWVELVSPRGDFTYRAPVVVLVNHWTGSMGE
                     GIAIGMDGMKRATIVGTEMACLVGATSGIKLPHTGIGVNFPTEKLFHVNGTPREKFVP
                     SVHVDLVKRDNQHGDAVMTRGLAVVRNMIKRSRGSQ"
     gene            complement(3795..4295)
                     /locus_tag="VFV61_03275"
     CDS             complement(3795..4295)
                     /locus_tag="VFV61_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899523.1"
                     /translation="MFQNPLTTKIVDFLLAIGIPVLPSQLGGECFLPGIRVETGKLLV
                     DEERLKYPGDLLHEAGHLAVAPGEVRPGLSGEVVIPGADMSTVEVQATAWAYAAVTHL
                     GLDPSLLFHEGGYAGKSKGLIFTYGLGVYPGANTLQDIRMTALGEMARSLGVPPYPHM
                     LKWIRE"
     gene            complement(4478..4783)
                     /locus_tag="VFV61_03280"
     CDS             complement(4478..4783)
                     /locus_tag="VFV61_03280"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX4899524.1"
                     /translation="MYSKDNLAKLKKMDSLAPEVMKAFWAFDKAAVAEGAISVKHKEL
                     IAVAVAVTTQCPYCIDIHSGNARKAGATDAEIVEAAMVAASLRAGGAVTHATHALSI"
     gene            complement(5176..5994)
                     /locus_tag="VFV61_03285"
     CDS             complement(5176..5994)
                     /locus_tag="VFV61_03285"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX4899525.1"
                     /translation="MSAPNTEMESLKTKLKAMWMSGDFGQIAKQLEAEAEAFINRLGL
                     KPGERVLDVACGSGNLTIPAARAGAEVTGADIATNLLEQGRARAAAEGLTIQFDEGDA
                     ESLPYEDASFDVVVSMFGAMFAPRPQLVAAELVRVCRPGGRIAMANWTAEGHIGQMFK
                     TTGKHVPPPPNMPSPIKWGDEATVRELLHDGVADLKLTRRMCLFNLPFSPAEVVEHFR
                     TYYGPTQRAFAALDSEGQAALRSDLEQLWAKNNQATDNTTKVDGEYLEVVATRS"
BASE COUNT         1407 a         1748 c         1589 g         1495 t
ORIGIN      
        1 gatttgtatc cgcagattat tgagtggttt gagaagggga attaaccgct actttgagtc
       61 tcccaatgaa ttcagcaccg tagttacctg gaagaggtga gtcgccagcc gctgcctgtc
      121 cgcgaccgca gagcgatctc gaaactgcac gaaaaaaggg cctaacagag cgccgttgtc
      181 gtttcgctcc tgccggcgca gaccctaagg tcgtcaactc gcatccggcg gggcggtcat
      241 ccgctttata tgagcattac cccagccgcg aatactctcg agcaagggca acaaggtgcg
      301 accatagtcg gtgagtgaat attcgactgg ggccggaacc gcacccttgg gatcacgttg
      361 cacgacgtcg tcatccgcga gctcacgcag ttgttgggtc aaaaccttct gcgaaatgtc
      421 cggcatcagt tttcgcagcg ccgcgaagtg cttcggcgat tccgccagcc aatacacaat
      481 aatcaacttc catttgccgc cgaccgcggc gagggcggcc gtcaggggac agccactcac
      541 cggattacgc cgccgtgaat aacgactatg gttacctaaa ggtgccttct tgagggaatt
      601 catagcccag actatattcc tgcccgcgtc ggctacaaga tcaatcatcg cagcattgag
      661 gagtcagcca tgatagtaga agtccgcagt taccgaatca agcccggccg tcgcgccgag
      721 ttcattcact tgtttgaaac gcgagccgtg gccgcactca gagcgcaggg aatgaaagtg
      781 gtagggccgt ttctcgatct cgagaacccc aacaagtttg tttggttgcg cagttttcct
      841 tcgctggatg aacgcgaacg aatgaggaac gcgttttacg aaggtgattt gtggaagaac
      901 gaactcgaag ctatcgcgat gccaatgctt gaaagttacg acgtcacgct ttgcgaaaca
      961 tctccaggat gcgtgtttga tttaccgcag actatcaatc aatgatcctt tccaaggtcg
     1021 gagaaattat cgtcatgaac agaggcagac attttccctt agcgcttttg ttattgactt
     1081 ccctttgcct gacgtcgccg tcagtgaacg ctcagcccga gtcagccgca acgccacagc
     1141 gcgatgggca acacgacttc gacttcgaat ttggcggttg gaaagctcac atctcgcgat
     1201 tgctaaaacc gctgacagga tccaaaacct ggatggaata cgatggcact tccgtcgtgc
     1261 gcaaggtctg ggatggtcgc gctaacctgg gagaactaaa tgtcagtggt ccgcaaggtc
     1321 atatccaggg cttgagccta cgtacctaca atccgcaatc ccatcagtgg cacatcggtt
     1381 gggtcaacag cgccgacgga acaatgggac cgcccatgat cggtggattc aagaatggtc
     1441 gtggcgagtt ttacaatcag gagtcattca atggccgggc catctttgtg cgctttatct
     1501 tttccgacat cactgcgaat tcgttccaaa tcgagcaggc gttttcagac gatggcggca
     1561 agacctggga ggcaaactgg atcgcgaaat tctcgcgggt gaaaggatag agttccgctt
     1621 aacaagagac accctgagct aataggcttg gaaataatac agggccaggg cggtaacggg
     1681 cacaggtttt ccgtcaaccg tggtcggctt atatgtcgtt ttcagcatcg cctcttccga
     1741 tgcttcgatg acaaattgat ctccacagat cgtcttcgcc gctatcactc tgccctgctc
     1801 gtcaacctgt atcttcatga ttaatgagcc gaagacatgg cgggatcggg gagtaacagg
     1861 cgagggccca ctgatccgct tatttatcag tagcccacct ctgcgtaaat cgtcgatcgg
     1921 gtgattcgcc gcttcgtcga agtcgaaacc gttttcgagc ttgtacagcc gcttctgtaa
     1981 tgcaaacgcc tcttcgaagt ccttgtactc tgttcttaga ctactaagta acaaacagat
     2041 gttccgggtg agagccgaga tcatcacctt gcgcgtgtca cgagcgtcaa cgtcgcgatt
     2101 cttgtcgtag gtaatcagat aacgtcgata ggttctcaac gcctcgttgc gcagaccccg
     2161 gacgtactgt agctctgcca cattagctaa cgcatccgcc actaagaagt gctctgggcc
     2221 gagagctttt tctctaagca tcagtactcg tttggcgagc gaaagagcct tgtcatactt
     2281 ccgctcgtga taaagagtta tgacttgagt gctcaattct tccgcctccg ccaactcggc
     2341 agactgagtc gtcgccggtg cagctgcttg ctgctgggta ggggccgagc taagggatga
     2401 agtcagagtg agcgagataa ctacaccagt gagtaacagg atctggaatt tcattattga
     2461 gacccccgcg aacgtttaat catattcctt actactgcca gccctctcgt cattacagcg
     2521 tcaccatgct gattatccct cttcaccaaa tcaacgtgga cggacggcac aaacttctcg
     2581 cgcggggttc cattaacatg aaacaacttt tcggtcggga agttcactcc gattcccgtg
     2641 tgcggcagtt tgattccgga tgttgctcca accagacacg ccatttcggt accgacaatg
     2701 gttgcccgct tcattccgtc catgccgatc gcgattcctt cgcccatact accagtccaa
     2761 tggttcacta agactacgac aggagctctg taagtgaagt ctcctcgcgg cgacaccagc
     2821 tcgacccaac tacgcctgat tccgtaccga tgttcttctg cgggcagact gtgtttttga
     2881 taaggcatgt cgcggctaat gaaacgactc atgatcccac gcgcgaccgt cgtgttacca
     2941 ccgctgggcg tatcacgcag atcaagtatc aagccgcgcg tatttcttaa agaggcgagc
     3001 gcaaggtcaa actgcttaat taattcggtg tcgccgagag aattatttat ccgaatgtaa
     3061 cccagatcgt tttggagttt gctgcgctca agcaagaatt tttgcgcagc cgctgcctca
     3121 gcactctcat tgtcaatact aagcacgctc cgatctccgt tagtcataac ctcgacactt
     3181 cttttctcat tatggcgtcc agccagcaga attctcagtg cccaatctct tgccggctta
     3241 tcgaccgaac gcagagactt gcctgtacgc cggctcacag cctcggcaac ggcaacgcca
     3301 ttaatggaaa caatctgcat tccaactttt attctcgctt tttcagcaag cgattcgggt
     3361 cttacttccg tgatgattgc cctgtcctga atccattctg cccacatgtc agcgccggag
     3421 gggacgagtc gggacgagga tggggtgttt gtgttcagat gtgtgtgaaa gtcataaagc
     3481 tcttcgagga cagattccaa cagccccacg aaatcgtcgc gtgttctgac cacactgacc
     3541 tgaggccggt aaatctcgcg cactctttgc cagtcggttt gcttttgatc gaagtaagcg
     3601 tagtcgtctg agaagctctg ccagaggtag tcaaagtcct cttcatattg ctgacgcgta
     3661 aagttggttt gcggcgcgga gacggcttgg gcaaagaaac ctaacaagac gagtagaacg
     3721 gagagtttta ccctaggcaa ttcagttctc cattagccaa cgccgcaggc gttttgattt
     3781 agaagaactt accctcactc cctgatccat ttcaacatgt gagggtatgg cggaactcct
     3841 aagctgcggg ccatctcacc taaagcggtc atcctaatat cctgcaaagt attggctccc
     3901 ggataaactc ccaggccata ggtaaagata agtcccttgg actttcctgc ataccctcct
     3961 tcgtgaaaca acaggctggg atccagaccg agatgagtca ccgccgcgta ggcccaggca
     4021 gtggcctgca cctccactgt gctcatgtca gcaccaggaa taactacttc tccgctcaaa
     4081 ccaggacgca cctcgcccgg cgctacagcg aggtgccctg cttcatgcaa taagtcaccc
     4141 ggatatttca atctctcctc atcaacaagg agcttgcccg tctcaactct aatgccgggg
     4201 aggaagcatt cgcctcctag ttgtgaaggc agcaccggaa taccgatcgc caatagaaaa
     4261 tcaacgatct tcgttgtcag gggattctga aacatcacga gtcatggccc atgacttttg
     4321 aggcaaagcc attctctaag tgctcgaaaa caacctgaca ggcgcaactg tcagccccgg
     4381 ttcaactatg aacgccgcgg tccttgaagc aagcggaatt aaacttccac ccctacgcgg
     4441 gatagatgat tgcatccggg acgcagcgtc gttgcgttca gatggacagg gcgtgcgtcg
     4501 cgtgcgtgac tgcccctccc gctcgaagtg aggcggcgac catcgccgct tcgacaatct
     4561 ctgcgtcggt tgcacctgct ttgcgcgcgt taccagaatg gatatcgatg caataagggc
     4621 actgagtagt gacagcaacg gcgacggcaa ttagttcttt atgctttacg gaaattgctc
     4681 cctcggcgac cgctgccttg tcgaatgccc agaaggcctt cattacttcc ggcgccaggc
     4741 tgtccatttt ctttagcttc gctaagttgt ctttgctgta catagttttg gtctctctct
     4801 ctctctctcg ttctggcaat aatataattg actcaatttc agcggaatca aacacacgtc
     4861 tgagagtgcc aagtgaggtc cgcaggaccg tcatcaaata gccacggccg tgaggcagtg
     4921 gtcagtcgaa acctaaatag tcgagcgccg aaggtgcggc agtaaacccc catcacagtg
     4981 cagaggaatg ccggccctgc gggcctcgat ttcatcttca ggccttcgga tccacggcct
     5041 tacggccgcg gctattgaat gccgcgccgg cggcgctcct ggaacccaaa ccacgatgac
     5101 cttatttggt gagtcaggta tgtgattgcc ctctttctct ttctctctgt ctcgtaaaca
     5161 ggcagtttga acccgctaac tcctcgtcgc gacaacctcg agatactcac cgtcgacctt
     5221 tgtggtgttg tctgttgcct ggttattctt agcccaaagc tgctccaggt cgcttcttaa
     5281 ggccgcctgc ccctcgctgt cgagcgctgc gaacgcgcgc tgcgtgggac cgtaataggt
     5341 gcggaaatgc tcgacgactt cagcgggtga gaagggaagg ttgaacaagc acatccggcg
     5401 cgttagtttc aaatctgcaa ctccatcatg tagcagttcg cgcactgtgg cctcgtcacc
     5461 ccacttgatc ggggacggca tattgggcgg gggcggcaca tgtttgccgg tggtcttgaa
     5521 catctgtcca atgtgtcctt ccgccgtcca gttggccatg gcgatcctgc cgccgggtcg
     5581 gcaaacacgc accagttcag cagctaccaa ctgcgggcga ggggcaaaca tagctccgaa
     5641 catgctcacg actacatcga agctcgcatc ctcgtaggga agactctccg catcgccttc
     5701 atcaaactga atcgtcaggc cctcagccgc agcgcgggcg cggccctgtt ccaacaggtt
     5761 cgtggcaatg tcggcaccgg ttacttcggc acccgcgcgg gctgcgggta ttgtcagatt
     5821 tccgctgccg caagccacat cgagcacacg ctcgcccggt ttaagcccaa gccggttgat
     5881 aaatgcttct gcttcggctt ccagttgttt cgctatttga ccaaagtccc cgctcatcca
     5941 cattgctttg agcttggtct tgagggattc catttcagta ttaggagcgc tcatggttga
     6001 tttcctttcc agaagataaa tcggctgttg ggtgtttggt tgcaattatg gcatagcccg
     6061 aattctaagc tgacttcacg gcgctgcaca tccggcggcg acctaatttc taaggacccc
     6121 tctggcccaa cgtgagaaac aatcccgccc agagtattaa ccatcccccg tctggtattt
     6181 gcagtagcag actttgaggc ggctgccagc cgtttccctc gcacttgata attatgctg
//
LOCUS       DASUEX010000197         5784 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_148049, whole genome shotgun sequence.
ACCESSION   DASUEX010000197 DASUEX010000000
VERSION     DASUEX010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5784
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148049"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="VFV61_03290"
     CDS             complement(<1..223)
                     /locus_tag="VFV61_03290"
                     /inference="COORDINATES: protein motif:HMM:NF013671.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HEX4899526.1"
                     /translation="MSANTTPTGTTSGAPMAPAMTLIVTEPAAAEIKKFMASEEGLPE
                     SAGLRVRVVPGGCSGFQYSLNIEEESRQGD"
     gene            complement(367..1449)
                     /locus_tag="VFV61_03295"
     CDS             complement(367..1449)
                     /locus_tag="VFV61_03295"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HEX4899527.1"
                     /translation="MSQLTPELILNALRQINDPDLNKDIVTLGFIKDLAIDGGNVSFR
                     IVLTTPACPVKDQMEGAARELVGAIPGVQTVSVRMDAEVPKGRGIGEKVSVPGVRNII
                     AVSSGKGGVGKSTVAVNVAVSLALDGARVGLMDADVYGPNVPIMLGASNAQPVVEGNK
                     LVPIEAYGVKIMSMAFLQPGDKPMIVRGPILHGLVKQFLSDVKWGELDYLVVDMPPGT
                     GDVQLSLAQLVPVQGAVLVTTPQEVAVADVRRALRMFETVAIPILGIVENMSYFIAPD
                     TGNRYDIFGQGGGERLAEMYHVPFLGAIPLGISVREGGDAGRPVVISEPNSFQAQAFR
                     RLAEEVARQVSIEAMKPELVVLGKGQ"
     gene            1646..2218
                     /locus_tag="VFV61_03300"
     CDS             1646..2218
                     /locus_tag="VFV61_03300"
                     /inference="COORDINATES: protein motif:HMM:NF013738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HEX4899528.1"
                     /translation="MPASFITQHFFAPVNVGHIDNPDGAGSAASVKCGAVLRVFLKVD
                     EAQRITAAKFKVAGCSYLVASCSVLSAALTGKTTGEAAVICQPPDSSIALLGQECPAE
                     KRDCQTLASQGFVAAIRNYSDAIRDEWSGAEALICTCFGVSEKTIETAIQSGGLRTIA
                     EVTKASNAGAGCRSCHPLIEDILDQIAREG"
     gene            complement(2234..4249)
                     /locus_tag="VFV61_03305"
     CDS             complement(2234..4249)
                     /locus_tag="VFV61_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899529.1"
                     /translation="MLALLYFALAIYFGDRISRSFFRFISVPHRLATSFLTGLLLSTL
                     ITYLGALAFARSSQPLVMGTITFLAAFALAIYKIPRYAAADYLNTYRERPVGSARWDW
                     ICCGAFLLFACWLMFATLNFKEGKFWIGFKGWTDFGANISLIQSFVFGHNFPTTHPFF
                     PGEPIRYHFLFWFQAANLEFLGLNPVWSINLLSIVSLLALLILIVTFAEILFNSRAVG
                     RIAALLFLFSSSLYYLPFLRSQESISGLLSSTLHRTDFLSSGYPWRGEDWGVLTISVL
                     GFQRHFISGIAILVVVLIFLVDRYQQNVNKIVSEDKGRTDGLGSNNPRTKNQLLALAI
                     DKPLLSASVFSGLLIGLLPFWNSAVFVAALAVLGCLLLLFSYRFYLASLLATAALIGL
                     PQVLLLRPRTVDQPIFHWGYTMDKPTIFQVLKYLGWAFGFKWLLILVALILLSKAHRK
                     FFLASLSLLVIVFTIQLSTDLFNNHKFLNLWGVFGSIFAAYGLWRIGKAHVAGTVLAC
                     VLALATIFGGIVDLFPIHNDPALTVPYENDRLTNWLLENTQPADVFLTHPLLTHPILF
                     TGRKIFLGNTLFAWSSGYRVGEREAIYVSMFQERNPVELVRLLNQNSIAYVAIDNGVR
                     SNSMIKHLNESVYQQHFEKVFDDTDRQYDLITIYKVPAAAPESAPTK"
     gene            4390..5580
                     /gene="nifS"
                     /locus_tag="VFV61_03310"
     CDS             4390..5580
                     /gene="nifS"
                     /locus_tag="VFV61_03310"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR03402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HEX4899530.1"
                     /translation="MDLIYLDNNATTRIAPQVLAAMQPYLSEHYGNPSSAHSLGRKMK
                     AAVEHARQQVADLIGAADVSEVVFTSCGSESDNWAIGGFLEQNPTRRHIITTRVEHEA
                     VRNLCEHLAQIGCEVTWLEVDGKGELNLEDLRKALRPDTGIVSVMLANNETGVLFPIA
                     EIGKIIRENSDAVFHVDGVQAVGKIPIDLKNLEVDLFALSGHKLNAPQGIGALYVRNG
                     VKLPPFIIGGAQEHGRRAGTSAVPNIVGLGAACELARLSDEHEEIERLRDKLEAEILK
                     RVSNARVNGVTTPGKRLPNTTNISFEYVEGENILLHLDREGICVSTGSACHSTTKVSS
                     PTLRAMNVPFTAARGSIRFSLGRFNTEAEIDRTLEVLPGIIESLLEMSPYEKSQVSSL
                     TSQV"
BASE COUNT         1494 a         1491 c         1545 g         1254 t
ORIGIN      
        1 agtcgccttg ccgcgattcc tcttcaatat tcagactgta ttgaaaaccg gaacagccac
       61 cgggaacgac gcggacacgg agtccagcgc tttccggcaa gccttcttcg ctggccataa
      121 atttcttaat ttcagccgca gccggctcgg tgacaatgag cgtcatggcc ggagccatag
      181 gggcgccgct ggttgtgccg gttggtgttg tatttgctga cattctttta aaaattctcc
      241 tttaatcaat tgccgattcc caattgccaa ttgccgatta gtgtaactgg atcgcctgta
      301 tgggtaaaag tctggtttac agttttgaca caatcggaaa tctgcaatcg ccaatcggca
      361 atgatgttac tggcctttcc ccaaaacgac aagttcaggt ttcattgcct caattgagac
      421 ctgccgggcc acctcctcgg cgaggcgacg aaaagcctga gcttggaacg aattcggctc
      481 gctgattact actggtctgc ctgcatcacc accttctcga acgctgatac cgaggggaat
      541 tgcccccaga aaaggcacgt gatacatctc cgcgagtctt tcgccgccgc cctggccgaa
      601 aatgtcgtaa cgatttccgg tatcgggcgc gatgaagtaa ctcatgtttt cgacgatccc
      661 caggatcggg atcgcgactg tttcaaacat ccgcaaagca cgtcgcacat ccgcgacagc
      721 aacttcctga ggagttgtga ccaaaacggc gccttgcacc gggacaagtt gagcaagtga
      781 taactgcacg tccccggtac cgggtggcat atcgacgaca agataatcga gttcacccca
      841 cttcacatct gaaagaaact gtttgacgag accgtgcaag atcgggccgc gtacgatcat
      901 cggcttgtcg cctggttgca ggaaagccat ggacattatc tttacgccat aagcctcaat
      961 cggaaccagt ttgttgcctt cgacgaccgg ttgcgcgttg ctggcgccaa gcataatggg
     1021 cacgttgggg ccatagacgt cggcgtccat caaacccacg cgggctccat caagcgccaa
     1081 cgaaacggct acgttgactg cgacggttga tttaccgacg ccacccttgc ccgaagacac
     1141 ggcgatgatg ttacgaacac cgggaacgga aaccttttcg ccgataccgc ggcctttagg
     1201 tacttcagca tccattctta cgctaactgt ctgcaccccg ggaatggcgc ccacaagttc
     1261 cctggctgcg ccttccattt gatctttgac cgggcaagca ggcgtggtaa gtacgatgcg
     1321 aaacgaaaca ttgccaccat caattgccag gtctttgatg aacccgagcg tgacgatgtc
     1381 tttgtttaag tctggatcat tgatctgccg cagggcatta agaataagtt ctggagttag
     1441 ttgtgacatg ttgttgttcg gctgaagtag gattatgcag cagggagttt tgaaaagtcg
     1501 aacgggggcc gtgcgatggc cgattgctga ctgccgattg acccggagct gagtgaggtc
     1561 gttaccagcg gctaccgccc ggtggaactg acttcgatcc caagctagaa tccttacttc
     1621 ttgcgaaacc tttgcatcac gactaatgcc tgcaagtttc ataactcaac acttctttgc
     1681 cccggtaaac gtgggacaca tagacaatcc agacggtgct ggtagcgctg catcggtcaa
     1741 gtgcggcgct gtgctgcggg tcttcttaaa agttgatgaa gctcaaagaa ttacggctgc
     1801 gaaattcaag gttgccggct gtagttacct cgtggctagt tgctcagtct taagcgcagc
     1861 gttgacgggc aagactactg gcgaagcggc ggtcatctgc caaccgccag attcctcgat
     1921 cgcgctgctg ggtcaggaat gtccggcgga gaagagagac tgccagacgc ttgcgagcca
     1981 aggattcgtc gccgcgatca gaaattacag cgacgccatc agggatgaat ggtcgggagc
     2041 tgaggcgctg atttgtactt gctttggagt ctcggaaaaa accatcgaaa ctgcgattca
     2101 aagcggcgga ctccgcacca tcgccgaagt cacgaaagct tcaaacgctg gggcgggttg
     2161 ccgttcatgt cacccattga ttgaagatat tcttgatcaa atcgcgcgcg agggttagga
     2221 aggctattcg tgatcacttc gttggcgcag attctggcgc ggcagcgggt accttgtaga
     2281 ttgttatcag gtcatattgc cgatcggtat catcaaagac tttctcaaaa tgctgctgat
     2341 aaaccgattc gttcagatgt tttatcatgc tgtttgaacg cacaccgtta tcgattgcta
     2401 cataggcaat gctgttctgg ttaagcagac gaaccagctc aaccggattg cgttcctgaa
     2461 acatagatac gtaaatcgct tcccgctcac ccactcgata gcccgaagac cacgcgaaga
     2521 gcgtgtttcc gaggaaaatc ttgcgacccg taaacaggat tggatgagta agcagtgggt
     2581 gggtcaggaa cacatccgct ggctgagtat tctccaggag ccagttggtc aatctatcat
     2641 tctcgtaagg gacagtgagc gcggggtcgt tatgaatggg gaatagatcg actatccctc
     2701 caaaaattgt cgccaatgcc agcacgcacg ccagcactgt cccggctaca tgagctttgc
     2761 cgatgcgcca taatccgtat gcggcaaaga tagagccgaa aacaccccag aggttcaaga
     2821 atttgtggtt gttgaaaagg tctgtgctca attgaatcgt gaacacaatg acaagcaaac
     2881 ttaatgacgc tagaaagaac ttgcggtgcg ctttggaaag aagaataagc gccaccagaa
     2941 tcagcaacca cttaaaacca aacgcccagc cgagatactt gagaacctgg aatatcgttg
     3001 gcttgtccat cgtatatccc cagtgaaaga tcggctgatc caccgttcgc ggtctgagca
     3061 acaaaacctg cggaagacct atcagcgctg ccgtggcaag cagcgacgca aggtaaaacc
     3121 ggtatgaaaa tagaagtaag agacagccga gcacggccag tgctgcaacg aagacggcac
     3181 tgttccagaa aggaagcagg ccgatcaata agccggaaaa aaccgaagcg cttagcagcg
     3241 gcttatcgat cgctaatgcc agcagctgat tcttagtacg cggattatta cttccgagac
     3301 cgtccgttcg gcccttatcc tcgctgacaa tcttattaac attttgctga tatcggtcga
     3361 ccagaaaaat cagcacgaca acaagaatcg caatgccact gataaagtgt cgctggaaac
     3421 cgaggacgct gattgtcagc acgccccagt cttcgccccg ccacggataa cccgacgaca
     3481 gaaaatctgt ccggtgaagc gtcgagctga gaagaccgct gatactttct tgagaccgca
     3541 ggaacggcag gtagtaaagc gagctcgaaa acaagaatag tagagcagca attctcccaa
     3601 ccgcccttga gttgaagagg atctccgcga aggtcacgat gagaatcaag agcgccagca
     3661 acgaaacgat gctcagcaga ttgatactcc agacggggtt aagtccgagg aattcgagat
     3721 tcgccgcctg aaaccagaaa aggaagtggt agcggatcgg ttcacctgga aagaatgggt
     3781 gggtggtggg aaagttgtga ccaaaaacga agctttggat taacgagata ttggcgccga
     3841 aatcggtcca gcctttaaag ccaatccaaa acttaccttc tttgaaattc agcgttgcaa
     3901 acatcagcca gcaggcaaac agcaagaacg ctccgcagca aatccagtcc caacgggccg
     3961 agccaacggg acgctcgcgg taagtgttaa gataatccgc tgctgcgtat cgaggaatct
     4021 tgtagattgc cagggcaaac gctgcgagaa aagtgatggt gcccattacg aggggctgac
     4081 tagatcgtgc aaacgcgagc gcgccgaggt atgtaatcag cgtactcagc aataagcccg
     4141 taagaaatga agtagccagc cggtgtggga ctgagatgaa gcgaaaaaaa gagcgactga
     4201 tgcgatcgcc gaagtaaatg gccagtgcga aatagagaag ggcgagcatc actactcctg
     4261 gagtttcaag cctgctgcaa acaagcttcg cctggaaaca gaagtgcaca accggtttct
     4321 cgcgaggccc gcgagtataa cagggcgtct cccctggaga acaagactca ctggtaaacg
     4381 gaaggccaaa tggacttgat ctacttagac aataacgcta ccacacgcat cgcaccgcag
     4441 gttcttgctg cgatgcagcc ctatctctcg gaacactatg ggaatccatc ttcggcgcat
     4501 tcactgggac gaaaaatgaa ggcagccgtt gagcacgcgc gccaacaggt ggcggatttg
     4561 atcggggccg ctgacgtaag cgaagttgtc ttcacctcct gtggctcaga gtcagataat
     4621 tgggccattg gcggttttct ggaacagaat cccactcgca ggcacatcat caccacacgc
     4681 gtcgaacatg aggccgtacg taatctctgt gagcatctcg cgcagatcgg ttgcgaagta
     4741 acgtggctgg aggttgacgg aaaaggcgag cttaatcttg aggatctgcg gaaagccttg
     4801 cgacccgaca ccggaatcgt ttccgtgatg ctcgccaaca atgaaaccgg ggtgttgttt
     4861 cccattgcgg agattggcaa gattatcagg gagaactctg atgctgtgtt tcatgtggat
     4921 ggagtacagg ctgtcggcaa aatcccaatc gatttgaaaa acctggaagt agatctcttt
     4981 gcactttcag ggcacaaact caatgcaccc cagggaattg gcgcgttgta cgtgcgcaac
     5041 ggtgtgaagt tgccgccatt cattatcggt ggcgctcagg aacatggccg ccgcgcgggc
     5101 acgtcagcag tgccaaacat tgtagggttg ggagccgcgt gtgagttggc ccgactctcc
     5161 gacgagcatg aagaaatcga acgactgcga gacaagttag aagctgagat tcttaagaga
     5221 gtttccaacg cccgggtcaa cggcgtcacc acgccgggaa aacgtcttcc caacacaacc
     5281 aacatttcgt ttgagtacgt ggaaggcgag aacatcctgc tgcatctcga ccgcgaggga
     5341 atctgcgtct cgacgggatc ggcatgtcat tcgacaacca aagtttcatc gccaacattg
     5401 cgcgcgatga acgttccctt cacggcggcg cgcggttcaa tccggttttc acttgggcgt
     5461 ttcaacaccg aggccgagat cgatcgcaca cttgaagtgt tgcctgggat cattgagagt
     5521 ctgttggaga tgtcgcctta cgagaaatct caagtctcaa gtctcacgtc tcaagtttga
     5581 ggggctggta cttcaattcc ctgctgcttt gtgtggtttc atggatcgta ttcctcgagc
     5641 aacagcgatc ttgagacctg agacctgaaa cctgagacct gaaacctgaa acctgaaacc
     5701 tgaaatgaga tctgtgaccc atatcacacg agactgaaac gcgcccgaga cccgacttcc
     5761 aacatctgac acccgagagc cctg
//
LOCUS       DASUEX010000198         3040 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1165563, whole genome shotgun sequence.
ACCESSION   DASUEX010000198 DASUEX010000000
VERSION     DASUEX010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3040
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1165563"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1153
                     /locus_tag="VFV61_03315"
     CDS             <1..1153
                     /locus_tag="VFV61_03315"
                     /inference="COORDINATES: protein motif:HMM:NF015901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HEX4899531.1"
                     /translation="GRDLEKAEAVGNGELFVDPVVQNAAADRKTLTAPRFDCDFFESG
                     NLARTFTATGGAKAVIEPVIKSETRGVRTLTGKKIAAVFVKETQDVERMDAQDDAKFN
                     ENDRNGVAANISYVAADDMIRMRGGEPTVWDARARTKAIELDSNLRDHVSYSRGKTAT
                     TYYSQEQTNGATPFRKVKSPVYVVSDRGEFHHDSGVAIYSGNARAWQDDNFVRGDKLT
                     IYRDEKKMDASGRVQSAVYNARRKDSGAIVPVFATADSMFYSDPNRLLHYESNVDIRQ
                     GTDRITSGVADVYLNKDTNEMEKTVAQRNVVLTQPNRKGTGDWAEYTTVNEVAVLKGN
                     PARVEDADKGNTEGARLTVFMREDRVVADDPRGPQSPGRVRSTHKVKKN"
     gene            1256..2062
                     /gene="lptB"
                     /locus_tag="VFV61_03320"
     CDS             1256..2062
                     /gene="lptB"
                     /locus_tag="VFV61_03320"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HEX4899532.1"
                     /translation="MAITENKKDGFIKLDSVLAERYAQPAPSAFALAASHLQKTYSRR
                     RVVDDVSLHVEKGEVVGLLGANGAGKTTTFYMIVGLEHPDSGAVQLGERDVTQLPMYL
                     RARLGLGYLPQEPSVFRRMTAAQNILAVLETMGLRRRERLTRLEELLEEFGIAQVRNT
                     RGDALSGGERRRTEIARALATEPQYILLDEPFAGIDPKAVNDIQNVILYLRNRGIGLL
                     ITDHNVRETLGVTDRAYIMAEGKIFRSGPPKELTEDAEVRRLYLGEKFRM"
     gene            2155..>3040
                     /locus_tag="VFV61_03325"
     CDS             2155..>3040
                     /locus_tag="VFV61_03325"
                     /inference="COORDINATES: protein motif:HMM:NF016827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899533.1"
                     /translation="MSLKLTTNLSQRLVLTPQLRQRIEMLQMTTLELSDLIQQQLLEN
                     PVLEEVATQEEAQELAEKILDHLASADAAADEPRVGAVEPELGAASSNGSGDTGSLVA
                     PDSENEAGDFEAHGSADPSEDSIGDDIVGEEASRDAFDEIDFGREFQDYLDPGYKTQE
                     VEYKEDAPTFEQFLTKPPSLADHLEWQLHMSPMEAEKCEAAICVIGNLDADGRLSATN
                     EEIAQMGGWTEELVEETRQAVMRLDPVGCGARDVKESLLVQLEVRGESDRLASRLIND
                     HLQDLQQHKLPHLSKQIGI"
BASE COUNT          756 a          778 c          895 g          611 t
ORIGIN      
        1 cggtcgcgat ttggaaaaag ccgaagcggt tggtaatggt gaattgtttg ttgatccggt
       61 cgtgcaaaat gccgccgcag atcgcaagac actaacggcc ccgcggtttg attgcgattt
      121 ttttgagtcg ggaaacctgg cgcggacctt tacagccacc ggtggcgcta aagcggtaat
      181 tgagcccgtg atcaagagtg agacgcgtgg cgtccgcaca ctgacaggga agaaaattgc
      241 agcagtgttt gtaaaagaaa cgcaggacgt cgagcgcatg gatgctcagg acgatgccaa
      301 gttcaatgaa aacgatcgaa acggtgtggc ggcaaacatc tcctacgtgg cggctgatga
      361 catgatacgc atgcgaggcg gcgagcccac ggtttgggat gcgcgcgccc gaaccaaagc
      421 tatcgaactg gattcaaatc tgcgcgacca cgtctcctac agtcgcggta agactgccac
      481 cacctactac agtcaggagc agaccaacgg cgcgactccg tttcgcaaag tgaagagtcc
      541 ggtttatgtg gtcagcgacc gcggggagtt tcatcacgac agcggcgtcg caatttatag
      601 cggcaatgcg cgcgcctggc aggacgataa tttcgtgcgg ggcgacaaac tgacaatcta
      661 tcgcgacgaa aagaaaatgg acgccagcgg tcgcgtgcaa agcgcagtct acaacgcgcg
      721 acggaaggat tccggtgcga tcgttcctgt gtttgccacc gctgattcga tgttctattc
      781 cgacccaaac cgtcttttgc actacgaaag caatgtagat attcgtcagg ggacagaccg
      841 gattaccagc ggcgttgccg atgtctacct caacaaggac accaacgaga tggaaaaaac
      901 cgtcgcgcag cgaaatgttg tcttgaccca acctaaccgg aaaggcaccg gggattgggc
      961 cgaatacaca acagtgaacg aggtggcggt gttgaagggc aacccggcac gggtggagga
     1021 cgctgacaaa gggaatacag aaggagctcg tttgacggtt tttatgcgag aagatagagt
     1081 cgtcgcggat gacccgcgtg ggccgcagtc acccgggcgt gtccgctcaa ctcataaagt
     1141 aaagaagaac tagctcaata ccgagttgat tgccgtcgcg cattcgtcgt gtaacgcaaa
     1201 ctgttagttt gcgaatttac ctgcgcaaac aggttgcgct agcgagtgga aagtcttggc
     1261 gatcactgaa aacaaaaaag acggatttat taagctggat tcggtgctag ccgaacgcta
     1321 cgcgcaacca gccccttcag ctttcgccct cgccgcttct catttacaga aaacttatag
     1381 ccgcaggcgc gtggttgatg atgtgagcct gcacgttgag aagggcgagg tagtcggttt
     1441 gttaggcgcc aacggtgcgg gcaagaccac gacgttctat atgatcgttg gtctggagca
     1501 tcctgattca ggtgcagtgc aactgggaga acgcgatgta actcaactgc cgatgtattt
     1561 gcgcgcccgc ttggggcttg gttacctgcc gcaggagccc tcggtgtttc gcaggatgac
     1621 ggctgcccaa aatattcttg ccgtattgga aacgatgggc ctgcgacggc gcgagcggct
     1681 gacgaggtta gaggaattgt tggaagagtt tggtatcgct caggtgcgca acactcgcgg
     1741 cgacgccctc tctggcggcg agcgaagaag aactgaaata gctcgcgcac ttgcaactga
     1801 accgcaatat attctgttgg acgagccttt cgccggcatc gaccccaaag ccgtcaacga
     1861 tattcaaaat gtcatcctct atctccgcaa ccggggaatc gggcttctga tcacggatca
     1921 caacgtccgc gaaaccctcg gcgtgacgga ccgcgcctat ataatggcgg agggcaaaat
     1981 atttcgctcg ggacctccaa aagagttgac agaagacgca gaagttcggc gactatattt
     2041 aggcgaaaaa ttccgcatgt gaaagtgccg cgcaaacagt cccggaatcg gttataatct
     2101 tctcgcgcat ccaggcgcaa aacaaaatca caacagtcgc ttttgtccta aggaatgtct
     2161 cttaaactaa ctacaaacct ctctcaacgt ctcgtgttga cgccccagtt gcgccagcgc
     2221 atagagatgc tgcaaatgac gacgctcgag ttgagcgatc tgattcaaca gcagttgctt
     2281 gagaatccgg tgcttgagga ggtggcgacc caggaggaag cccaggagct ggcggaaaag
     2341 attttggatc acctcgccag cgccgatgcc gcggcggacg agccgcgcgt cggggctgta
     2401 gagcccgaac tgggcgcagc gtcttctaat ggctcgggtg acacgggaag cctggttgcc
     2461 cctgacagcg aaaatgaagc tggtgacttt gaggcccacg gttcggcgga tcccagtgag
     2521 gattcaattg gtgatgacat cgttggcgag gaagcctccc gcgacgcgtt tgacgaaata
     2581 gatttcggtc gtgagttcca ggattatctc gatcccggtt acaagacgca ggaagttgaa
     2641 tacaaagaag acgcccccac cttcgaacag ttcctaacca agcccccatc cctggctgat
     2701 catctcgaat ggcaactgca catgagtccg atggaagcgg agaagtgtga ggccgcgatc
     2761 tgtgtgattg gaaatctgga tgccgatggg cgactcagtg cgaccaacga ggaaatcgcg
     2821 cagatgggag gctggacaga ggagctggtc gaggaaacgc gacaggctgt gatgcgtctc
     2881 gacccggtcg gttgtggcgc ccgcgacgta aaagaatcct tgttagtgca gcttgaagtg
     2941 cgtggagaaa gcgaccggct cgcctccaga ttgattaacg accatcttca ggatctacag
     3001 cagcacaagc tgccgcactt atccaaacag atcggcatcg
//
LOCUS       DASUEX010000199         2595 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1529283, whole genome shotgun sequence.
ACCESSION   DASUEX010000199 DASUEX010000000
VERSION     DASUEX010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1529283"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /locus_tag="VFV61_03330"
     CDS             complement(<1..370)
                     /locus_tag="VFV61_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899534.1"
                     /translation="MSITTLKIRLSMRVALSIALTAAISATVLSQQPGSQHAPSAERL
                     RAHVTYLASDKLEGRRTGTKGANAAGDYIAKEFASLKLKPGASKASYRQSFPYVAGLE
                     LGKTNKLQFSDNGPNLRVGED"
     gene            complement(485..1558)
                     /locus_tag="VFV61_03335"
     CDS             complement(485..1558)
                     /locus_tag="VFV61_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899535.1"
                     /translation="MTRTKQLTLLTGCALTILSFCLVSAYTKPVATPALVAAPVEKHL
                     RNVTQLTFGGENAEAYFSADGRRLIFQSTRENHGCDQIYTMNVDGSNVRMVSTGTGRT
                     TCSYFFPNQGRILYSSTHLGSKDCPPRPDFSQGYVWAIYPTYDIFIARPNGSELKQLT
                     QTPGYDAEATISTSGKKIVFTSTRDGDLDIYSMDANGRNVRRLTNEIGYDGGPFYSRD
                     GKQIVYRAYHPQSEKDKADYQSLLKANLIRPTTLEIWVMNADGSNKRQITQNGKANFA
                     PYFFPNGKRIIFASNMDDPKGRNFDLYTIKVDGSGLERITDNDTFDGFPMFSPDGKKL
                     VFASNRNAKERGETNVFIADWVE"
     gene            complement(1601..2434)
                     /gene="lgt"
                     /locus_tag="VFV61_03340"
     CDS             complement(1601..2434)
                     /gene="lgt"
                     /locus_tag="VFV61_03340"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEX4899536.1"
                     /translation="MYPELFRIGNFPINTYGVFLALAFLGAIMITVRLGARDGLPRER
                     IYDLSLWMLLAGLIGSKILMFWVEPEYREHPLQLLSLDFLRSGGVFYGGLIGAVVAGY
                     FLMKRYKLPWWKTADACAPGIALGNFFGRQGCFAAGCCWGKPTTLPWGVQFTELGHSI
                     TGVPIDVHLHPVQLYESFSMLIVFFFLLWLHKRKRFSGQIILAYALLYSLVRFAVEFV
                     RDDPRGDLLGLTTLTGLSTSQMISLIVAVSALIAFVIRRRKSGDLAKGDRIATPTTAR
                     A"
BASE COUNT          620 a          644 c          732 g          599 t
ORIGIN      
        1 agtcttcacc aacccgcaga tttggcccgt tatcagaaaa ctggagttta ttagttttgc
       61 ctaattcgag tccggcgacg taggggaacg attgcctgta actggccttg ctagcgccgg
      121 gtttgagctt aaggctggca aactctttcg cgatgtaatc tcccgcggcg ttcgcaccct
      181 ttgtcccggt gcgtcgtcct tcgagtttgt cggaagcgag ataagtgacg tgtgctcgga
      241 gacgctcggc gctgggagcg tgttgtgaac ctggctgttg cgagagaaca gtagcggaaa
      301 tggctgctgt taaggcaatg gacagggcga cgcgcattga gagtctgatt tttagcgttg
      361 taatgctcat gatttggttc cttgcagctt gaatctattc atcgttagcg ggggcaagcc
      421 gcccttcctt actgaaagtt tccctccttg aactctcgcc tctaaacctg aaactcgaaa
      481 cttcttattc cacccagtca gcaatgaaaa cattcgtctc gccacgttcc ttggcattgc
      541 ggttcgaagc gaacacgagt ttcttgccat cgggcgaaaa catggggaaa ccatcaaaag
      601 tatcgttgtc agtgatccgt tccaggccgg agccatcgac cttgatcgtg tagagatcga
      661 agttgcgtcc tttcggatcg tccatgttgg aagcaaagat gatccgttta ccgttgggga
      721 aaaaataagg cgcgaagttg gctttcccgt tttgcgtaat ctgccgcttg ttcgagccgt
      781 cggcattcat cacccaaatc tcgagcgtag tcggacgaat caagtttgcc ttcagcaggc
      841 tttgatagtc ggccttatcc ttctcgcttt gcgggtggta agcccggtag acaatctgct
      901 taccgtcacg agaataaaaa gggccgccat cgtagccaat ttcattcgtc aagcggcgta
      961 cgttccggcc attcgcgtcc attgaataga tgtccaggtc gccgtcgcgc gtggaagtga
     1021 aaacgatctt cttgccggaa gtggaaattg tagcttcagc gtcgtagcca ggtgtctggg
     1081 ttagctgttt caactctgat ccgttgggac gcgcaataaa gatgtcgtag gtcggatata
     1141 tcgcccagac atagccttgc gaaaagtccg gccgcggcgg acagtctttg ctaccgagat
     1201 gcgttgacga gtaaaggatg cgtccctgat tcgggaagaa gtaggagcag gtggtgcggc
     1261 cggtgccagt ggaaaccatc ctcacgtttg agccatcgac gttcatcgta taaatctggt
     1321 cgcaaccgtg attttcgcgg gtggactgaa agatgagacg ccggccatca gccgaaaaat
     1381 aggcttcggc gttctcgcca ccgaacgtta gttgggtaac gttgcgcaaa tgtttttcta
     1441 ccggcgctgc aaccagagcc ggagttgcca cgggttttgt gtaagcggag accagacaga
     1501 agctaaggat tgtcagggcg cagcctgtta gtagagttaa ttgtttcgtt cttgtcatga
     1561 tgaagagctg tgctggttat gatgtcaggg tgtctatgga ttaggcgcga gccgtggttg
     1621 gcgtcgcgat acgatcacct tttgccaggt cgccagactt gcgccgccga atgacaaaag
     1681 cgattagcgc gctcaccgcc acgatgagac taatcatttg cgaagtggag agaccggtca
     1741 gtgtagttaa gcctaacaga tcaccacgcg gatcgtcgcg tacaaactcg acggcgaagc
     1801 gaacgaggga ataaagcaac gcgtaggcga gaatgatctg cccgctaaaa cgtttccgct
     1861 tatgaagcca caatagaaag aaaaacacga tcagcatgga aaacgattcg tagagttgta
     1921 ccggatgcag atgcacatcg ataggaactc cagtaatgct atgcccgagc tcggtgaact
     1981 gtacgcccca gggcagtgtg gttggtttgc cccaacagca gccggccgca aagcaaccct
     2041 ggcgtccaaa aaaattaccg agcgcaatgc cgggagcgca ggcgtcggca gtcttccacc
     2101 agggcagttt gtagcgcttc atcaggaagt agccggcaac gaccgctccg attaacccgc
     2161 cataaaagac accgccagat cgcaggaagt cgagcgagag cagttgtaat ggatgttcgc
     2221 gatattctgg ctcgacccaa aacatcagga tcttggaacc gatcagtccg gcgagcagca
     2281 tccacaagct caggtcgtag attcgttcgc gcggcaaacc gtcgcgcgcg cccaggcgca
     2341 ccgtgatcat gatggcgccg aggaacgcga gcgccaggaa tacgccatac gtgttgatgg
     2401 ggaaattacc gatgcgaaaa agttctggat acatcttagt gattgccgaa tgccgattgg
     2461 cgattgttag tagtcagtag ttcgtaatca gtagtgactg gtagtcagcg taacgcgatg
     2521 acaaatggaa aatggcaaat ggcaaatgaa acagactcca gactgcggcc tccgacccac
     2581 tgctactgcc ttctg
//
LOCUS       DASUEX010000200         2010 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1282026, whole genome shotgun sequence.
ACCESSION   DASUEX010000200 DASUEX010000000
VERSION     DASUEX010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1282026"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..593
                     /locus_tag="VFV61_03345"
     CDS             <1..593
                     /locus_tag="VFV61_03345"
                     /inference="COORDINATES: protein motif:HMM:NF042562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6687 family protein"
                     /protein_id="HEX4899537.1"
                     /translation="GGEPVDDAKAYELILPEVERVITRVDAYEHLWRDGWNQVATAIE
                     SFEKGASTAAELDGVNTTLVTIAPEVFGPSGFDPKKHGVPVMAISRYAHGQLFLIAMP
                     YENGWSYRFDYPYYSWAETVVRPKIERGDLTMLLARLNEIEKNKKGTWQRDGSEMTSA
                     IKFLDGTNIPAASSLRPDEVAAIVRSEHRTRSAGVK"
     gene            630..1547
                     /locus_tag="VFV61_03350"
     CDS             630..1547
                     /locus_tag="VFV61_03350"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX4899538.1"
                     /translation="MHDVLIIGAGPAGLAAAFWCDQLGLDVLMLEQESEIGGQLHSIY
                     GPIENYPGLRAQDGREFLEGFAAGAAEAGFDLWTNVAIEKVDLTAKRVTLKSGEELCA
                     ITIVIATGVRRRQLGIPGEAEFAGKGIIESGARDKDLYAGKDVCIIGGGDAAVENALL
                     LSEACATVTLVNRSKKLRARREFTEQLASNHCVTVFNEAIATRILGSSAVAAVEIERK
                     QAIKPFQMAVKGVLIRIGVEPNSDMFRDQLGVDDRGYIVVTSQQETTVPNVFAIGDIS
                     NPLAPTISGAAGNGATVAKVIATRLNAGG"
     gene            1624..>2010
                     /locus_tag="VFV61_03355"
     CDS             1624..>2010
                     /locus_tag="VFV61_03355"
                     /inference="COORDINATES: protein motif:HMM:NF015690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HEX4899539.1"
                     /translation="MTSDTCGDRPPYTDAHIAAYPVEYIAGIDLYNAGEFHAAHDAWE
                     ERWMGEVGPQEKLFLQAMIQSAVAFHHLEIGRPGAARRMHQMAREKFARLGVNVFMSL
                     DLLDYQAQLEAALAWLRDAPDPRELVQ"
BASE COUNT          464 a          510 c          606 g          430 t
ORIGIN      
        1 atggtggcga gcctgtcgac gatgcgaaag cctacgaact gattctgcct gaagtggagc
       61 gggtgataac aagagtcgac gcgtacgaac atctgtggcg agatggttgg aatcaggttg
      121 ctacggcgat tgagagtttt gagaagggtg cctcgaccgc cgcagaactg gacggcgtaa
      181 acacaacgct tgtcactatc gcaccagaag tctttggccc atcgggtttt gatccaaaaa
      241 aacacggcgt tccggttatg gcgatttcgc gttacgcgca tggacaattg tttctgatcg
      301 caatgcccta tgaaaacggc tggagttacc gttttgatta tccatattac tcgtgggcgg
      361 aaaccgtggt gcggccgaag attgagcgag gtgacctgac aatgctgctc gctcgcttga
      421 atgaaatcga aaagaataaa aaaggcactt ggcagcgaga tggaagcgag atgacctcgg
      481 ctatcaagtt tctcgatggg actaacatac ctgctgcgtc gagcctgcgt cctgatgaag
      541 tcgcagcgat tgtccgctcg gaacatcgaa cgagaagcgc tggcgtgaaa taggtttgcc
      601 aattcaactc cagccttctg aaccagccca tgcatgacgt cttaatcatc ggcgccggcc
      661 cggcgggcct ggcggctgct ttctggtgcg accaactggg cctggacgtt ttgatgcttg
      721 aacaagagtc agagatcggc ggacagcttc actccatata cggaccaatc gaaaattatc
      781 ccggcctgcg cgcgcaggat gggcgggagt ttcttgaagg cttcgccgcc ggcgcagccg
      841 aggctgggtt tgatctttgg acaaatgtcg cgatcgaaaa ggtcgacttg acggcaaaaa
      901 gagtaacgct gaagagcggc gaagagctct gcgcgatcac gatagtgatc gctaccggcg
      961 ttcgacgccg tcagctcggg attccgggcg aggcggagtt tgcgggtaaa ggaatcatcg
     1021 agtccggcgc ccgcgacaag gacctttacg ccggaaagga tgtctgcatc atcggcggtg
     1081 gcgacgccgc tgttgagaac gcgctgctgt tgagcgaggc ctgcgccacg gtgactctcg
     1141 tgaatcgcag caagaagtta cgagcgcgcc gtgagttcac ggaacagttg gccagcaacc
     1201 attgcgttac ggttttcaat gaagcgattg cgactcgcat cttgggaagc tccgctgttg
     1261 cagcggtgga gattgagcgc aagcaggcaa ttaagccctt tcagatggcc gtcaagggcg
     1321 tcttgattcg aattggagtc gaaccgaact ctgacatgtt cagggatcag ctcggagtgg
     1381 acgatcgcgg gtacatcgta gtcacgagtc aacaggaaac caccgtgccg aatgtattcg
     1441 ccatcggtga tatctcaaat cccctggcgc cgaccatcag cggagctgct ggaaatggcg
     1501 ccaccgtggc aaaggtgatt gccacgcgct tgaatgccgg cggatagctc gaaatggagg
     1561 caagcacgct cagaataagg tcatttcaga tttcagtctc gcgtccaatt tagataagcg
     1621 gctatgactt ctgacacttg cggcgaccgt ccgccataca ccgacgcaca tatcgctgcg
     1681 tatccggttg agtacattgc ggggatcgat ctctacaacg ccggagagtt tcatgcggct
     1741 cacgatgcct gggaggaacg ctggatggga gaagtaggcc cgcaggagaa acttttctta
     1801 caggcaatga ttcagtcggc cgttgccttt caccacctgg agattggcag gcccggtgca
     1861 gcgcgcagaa tgcatcaaat ggccagagag aagtttgcac gcctcggtgt caacgttttc
     1921 atgtcgctcg atttgttaga ctaccaggcg caacttgaag ccgcgctggc ttggttacgc
     1981 gacgctcctg acccgcgtga gttagttcaa
//
LOCUS       DASUEX010000201         2028 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1408865, whole genome shotgun sequence.
ACCESSION   DASUEX010000201 DASUEX010000000
VERSION     DASUEX010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1408865"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1510
                     /locus_tag="VFV61_03360"
     CDS             <1..1510
                     /locus_tag="VFV61_03360"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX4899540.1"
                     /translation="ISQTQTGTDPKNPAPQVNAPSAPLPNNAEDCACESQVLPEDLAI
                     VNGVRITRQDIERVTRDPVNQLRRQVIEARKRELDLQINSKLLAIEAKKRGISSTKLL
                     EQEVFAKVKEPTEAEARVFYDQNKARIKAEFKDAREDVLQYLRDQRQREGAKKFAEGL
                     RATIETKLELAEAPPPKDDAERARVRAVVNGERITSGDIEDALKPIVFDVQQRVYKLR
                     KDELDLSINDTLLVQEAQKRKITTNGLLDAEVKPKTVTEEEARAFFEQNKDRVSGDFA
                     QTKDAILRYLLQIEVRRAEREFVEKLRAAASIQIFLVAPESPVFSISTADQPSLGNAA
                     APVTIVEFTDYQCPSCAATQPTLERLVKEYGNRVRLVARDFPLSQHPEAFKAAEAAEA
                     AREQGKYWEYREILMRNQSALGVVKLKDYAGELALDRVRFDQALESGKFAEMVQRDIE
                     DGMRLGIDSTPTVFINGRRMSDKSYEALKANIETALKSSTSGKSAEGGRRTF"
     gene            complement(1764..>2028)
                     /locus_tag="VFV61_03365"
     CDS             complement(1764..>2028)
                     /locus_tag="VFV61_03365"
                     /inference="COORDINATES: protein motif:HMM:NF024420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HEX4899541.1"
                     /translation="ASDISRGSMETRFIEVKGRAAVGEIALTANEYKTAQRLGDDYWL
                     YVVFNCASTPQVTTIQNPARFDWEPLSKIDCYRIGAEAILKSH"
BASE COUNT          527 a          536 c          552 g          413 t
ORIGIN      
        1 catcagccaa acacaaaccg ggacggaccc aaagaacccg gcacctcaag tcaacgcacc
       61 aagtgcccct ctgcccaaca acgccgagga ttgcgcatgt gagtcgcaag tgttacctga
      121 agatttggcg atcgtgaacg gcgttcgaat tactcgccag gatattgaaa gggtaacccg
      181 ggatccggtg aatcaactgc gacgccaggt catcgaagcg cgcaagcgag agcttgatct
      241 ccaaattaac tcaaaactgc tggccattga agcgaaaaag cgtggaataa gcagcacgaa
      301 gctgctcgaa caagaagttt tcgccaaagt gaaagagcct accgaggccg aggctcgggt
      361 tttctacgat cagaataagg cgcgtattaa ggccgagttt aaggacgcca gggaggatgt
      421 gctccaatat ctgcgcgatc agcgacagcg cgagggggca aagaaattcg cggaagggct
      481 gcgcgcaacg atcgaaacca agctggagct cgcggaagcg ccgccgccga aggatgacgc
      541 ggaacgtgct cgcgtgcggg ctgtagtcaa cggcgagcgc atcacttccg gagacataga
      601 agatgccctg aaaccgatcg tgtttgatgt tcagcagcgc gtttacaaac tacgtaagga
      661 cgagcttgac ctgagcatca acgatacgct tctggtgcag gaggcccaaa aaagaaagat
      721 caccacgaat ggcttgctcg atgctgaagt caagccaaaa acagtcaccg aagaagaggc
      781 gcgcgccttc ttcgaacaga ataaggaccg agtaagcgga gacttcgccc agacaaagga
      841 tgcgatcctt agatatctgc tacaaattga ggtacgcaga gccgaacgcg aatttgtcga
      901 aaaactgcgc gccgcagcct cgattcagat atttctggtg gctcctgagt caccagtctt
      961 ttccatctcg acagcggacc agccttctct gggtaacgcc gccgccccag tgacaatcgt
     1021 tgagttcacc gattatcaat gtccgagttg cgccgccacg caaccgactc tcgagcgtct
     1081 cgtcaaggaa tacggcaatc gggtaaggct ggtcgcgcgc gactttccgc ttagccaaca
     1141 ccccgaggcc ttcaaggcgg cagaagcggc cgaggcagcc cgcgagcaag gcaagtactg
     1201 ggagtaccga gaaatactga tgcgcaatca atcggcactg ggcgtcgtca agttgaagga
     1261 ctatgctggc gaactcgctc tcgatcgcgt ccggttcgat caggcgctgg aatcgggaaa
     1321 gtttgccgag atggtgcagc gcgacatcga agacggaatg aggctgggta tcgattcgac
     1381 gccgaccgta tttatcaacg gtcgtcggat gtcggataaa agttatgagg ctttaaaagc
     1441 gaatattgag accgcattga agagttcgac gtcaggaaaa agcgcggaag gtggacgtcg
     1501 aaccttctaa catttgcacg gctccagaga agattggctc cgcaggtagg acttcgagac
     1561 cgttcaaatt atcgcggctt tcggcgaact tcttatcttt gctaagaatt tcgcacattc
     1621 tgattttaca ttgttttcac cgtctacacc ttcagcactc cgttgtgcct tagttgtgac
     1681 aggcattccg tgttatcaat tccacaccaa gccggcattc gaacacgatg tgcagttgaa
     1741 tcgtttcaca gcacaacgaa tgtttagtga ctcttcaaaa tcgcttctgc cccgatgcga
     1801 tagcaatcaa tcttcgacag cggttcccaa tcaaaacgag ccggattctg aattgtggtt
     1861 acttgcggtg tcgacgcgca gttgaagacg acatataacc agtagtcatc gcccaggcgt
     1921 tgcgcggtct tgtattcgtt cgccgtaagt gcaatctcgc ccaccgcggc gcggcccttt
     1981 acttcaatga accgagtttc catggacccc cgcgaaatgt cagaggct
//
LOCUS       DASUEX010000202         3537 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1530287, whole genome shotgun sequence.
ACCESSION   DASUEX010000202 DASUEX010000000
VERSION     DASUEX010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3537
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1530287"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..566)
                     /locus_tag="VFV61_03370"
     CDS             complement(<1..566)
                     /locus_tag="VFV61_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899542.1"
                     /translation="MSDMNPQPSAASSSGSFSPPPPPAPVLEPPALRPLHLRKPAIVL
                     FVIGLLILIGGIAGFIAGGLQTGGAVCFWGVLLFAFSFIPLPKAGPDAPAPMSAFERI
                     AGVFYEPARVFNNLRSHPKWLAGLLVIVLLNIAYAVAFQQRLTPERIVNFTAEKMAQT
                     PMIPPEAVERTREQGLEQAKNPVQRVGTA"
     gene            complement(668..1687)
                     /locus_tag="VFV61_03375"
     CDS             complement(668..1687)
                     /locus_tag="VFV61_03375"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HEX4899543.1"
                     /translation="MENGSSAAVQTPQAHLVTVTGLVKHFPVEGSDDVVRAVDGVSFA
                     IMRGETLGLVGESGCGKSTVGRCLLRLIEPTAGQIEFAGRDVLALGKSEMRQLRREMQ
                     IVFQDPYASLNPRMTVGDIIGEPLVIHKIGTKTERRERVRSLLQRVGLNPDYSKRYPH
                     EFSGGQRQRIGVARTLALNPQLIVADEPVSALDVSVQAQVVNLLQELQAEFSLTYLFI
                     SHGLAVVEHISTRVAVMYLGRIVEIASAKDLYLQPLHPYTQALLSAIPIPDPTRTRER
                     IVLQGDVPTPINPPSGCRFRTRCPIAIDECAHVDPGLREVAAGHEVACIRVPGWEKAA
                     PRSET"
     gene            complement(1995..3077)
                     /locus_tag="VFV61_03380"
     CDS             complement(1995..3077)
                     /locus_tag="VFV61_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4899544.1"
                     /translation="MNTILTSTEIATPAVPHLLEVKNLRTQFQTRAGLVRAVDGVSFH
                     LDGGELLGLVGESGCGKSITALSVMRLVAPPGKIVAGEITFDGKNLLGLSDSEMRQIR
                     GDDIAMIFQDPMTSLNPVFTVGEQIAEALRLHRQLDRKAARAAAIDAMREVAIPDPAR
                     RADDYPHQLSGGMRQRVMIAMALACDPKLLIADEPTTALDVTIQAQILELLNELRKTR
                     ELAVLLITHDLGVVAEVADRVAVMYTGKIVEEAPVNELFARPRHPYTEGLLRSVPKLT
                     MEHVAKKERLETIEGVVPNPTDLPPGCHFAPRCQYRISRCEEGEIPLYELEGDVRVRC
                     VLFDLAAAVTADHHATAEDRTLTGDN"
     gene            complement(3052..>3537)
                     /locus_tag="VFV61_03385"
     CDS             complement(3052..>3537)
                     /locus_tag="VFV61_03385"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEX4899545.1"
                     /translation="QFLATKNRTYVFSALSSTLVNPDLNLQKFFESISFGSPTEGNVI
                     HEGPSEVQLLYSLSITKEGDPPPFVGREVTQKARVLSKPEPTYTEEGRQTQTTGTVVL
                     RAVFSETGEVIKIRALSGLPHGLTQKAIDAARQIKFIPAIKDGRFVSTHIQLEYNFNL
                     Y"
BASE COUNT          814 a         1000 c          953 g          770 t
ORIGIN      
        1 gcagtcccaa cgcgttgcac tggattcttg gcttgttcca aaccttgttc ccgcgttcgc
       61 tcaacagcct cgggcggaat catgggagtc tgggccatct tctccgcagt gaagttaacg
      121 atacgctccg gcgttagccg ctgctgaaag gccaccgcat aggcaatgtt caagagcaca
      181 atcacgagca gccccgcaag ccatttggga tgcgaccgca agttattgaa aacgcgagct
      241 ggttcgtaga agactccggc aattctttcg aaggcagaca tcggtgctgg ggcatccggg
      301 ccagcttttg gcaagggaat aaagctaaac gcgaacagga gcacgcccca gaaacacacg
      361 gctccaccgg tttgtagacc accggcaatg aagccagcaa tcccgccaat caaaatcagc
      421 agtcctatga caaataagac gatggccggc ttgcgcaaat gaagcggccg aagtgctggt
      481 ggttcgagca ccggcgcagg cggtggtggt ggtgaaaaag atccactgga cgacgcggca
      541 ctcggctggg gattcatatc gctcattaaa agttcctcct ggagtaagag gacggggaga
      601 cacgcttccg tccgcgatat tgttcggctg gcatcactgt cgatgcttga tacgtcggta
      661 ataaagtcta tgtttcagat cttggcgccg ccttctccca acccggaacc ctaatgcagg
      721 cgacctcatg gcccgcggct acctcgcgca gcccgggatc gacgtgcgcg cactcgtcga
      781 tcgcaatggg acagcgcgtg cgaaagcggc accctgacgg cggattgatc ggagtcggca
      841 catcaccttg cagcacgatg cgttcgcggg tgcgggtagg atcgggaatt ggtatcgccg
      901 aaagcaacgc ctgcgtataa ggatgcagtg gttgcaggta aagatcttta gcactcgcga
      961 tctccacaat ccgccccaga tacattaccg ccacacgagt cgagatatgc tcaaccacgg
     1021 cgagcccatg cgagataaaa agataagtaa gactgaactc cgcctgtagc tcctgaagca
     1081 gattaacgac ctgagcctgc acggacacat caagggccga caccggctcg tcagccacaa
     1141 ttagctgcgg attcaaagca agcgtgcgcg ccaccccaat acgctgccgt tgaccccccg
     1201 aaaattcatg agggtaacgc ttgctgtaat caggattaag cccgacgcgt tgcaacaagc
     1261 tgcggacacg ctcgcgtctt tctgtcttgg ttccgatctt atgaattaca agtggttcgc
     1321 cgatgatgtc gcccacggtc atgcgcggat tgagtgaggc gtagggatcc tgaaaaacga
     1381 tctgcatctc gcggcgcaac tgtcgcattt cgcttttacc caaagccagg acgtcacgcc
     1441 ccgcaaactc aatttgtcct gcggttggtt cgatcaaacg taagagacaa cggccgaccg
     1501 tcgattttcc acagcctgac tcacccacga ggccaagtgt ttcgccgcgc atgatggcga
     1561 aggacacgcc atcaaccgcg cgcactacgt cgtcactgcc ctctaccgga aagtgcttta
     1621 cgagcccggt gacggttacg agatgagcct gtggcgtttg gacagccgct gaagaaccat
     1681 tttccatttc tcattttcca tttccgattg gctcgatagt caaaagcacg gagacgctaa
     1741 ggcgcgaagg cgcaatgaaa cgattcaatg ctttacgtaa ttgcttggtg ccacttcgca
     1801 gatcgtcttc ttagccagca caaccacgat ccacaaaacc aagcgaaccg actctaccaa
     1861 gattgtgact aactctgcgc gccgcgacga agtggtcaca gagcattttt atggatttct
     1921 ttgcggctct gcgcctttgc tctctgcgtt tacttatgac gactgacgaa atagaaacga
     1981 catatgggaa atgttcagtt atcccccgta agcgtcctat cttccgccgt ggcgtgatga
     2041 tcggccgtga cggccgccgc caagtcaaac aacacacagc gcacccgcac gtcaccttcc
     2101 agctcataga gcggaatctc accctcctcg cagcgtgaga tgcggtattg gcagcgcggc
     2161 gcaaaatgac aaccgggagg cagatcagtc ggattaggaa cgacgccttc tattgtttcg
     2221 aggcgctcct tcttcgccac atgctccatc gttaacttgg gaactgaacg taatagacct
     2281 tccgtgtagg gatggcgcgg ccgcgcaaac aattcgttca cgggcgcctc ttctacaatt
     2341 ttccccgtgt acatgaccgc aacgcgatcg gcaacttcgg cgacgacgcc caggtcgtga
     2401 gttatcaaca gcaccgctag ctcccgtgtt ttgcgcaatt cattgagcag ttccaggatc
     2461 tgcgcctgaa tcgtcacgtc gagggccgta gttggctcgt cagcaatcag aagtttggga
     2521 tcacaagcta gcgccatggc aatcatcacg cgctggcgca tgccgccgga tagttgatgc
     2581 ggatagtcat cagcacgccg cgccggatct ggaatcgcca cttcccgcat tgcatcgatt
     2641 gccgcagcgc gcgctgcttt gcgatcgagt tggcggtgca gacgcagcgc ttctgcaatc
     2701 tgttccccga ctgtgaagac gggattgagc gacgtcatcg gatcctgaaa gatcatcgcg
     2761 atatcgtcgc cgcgaatctg acgcatctct gaatccgaaa gaccgagcag gttcttgccg
     2821 tcgaacgtga tctcgcccgc gacgattttt cctggaggag ctaccagacg catcaccgag
     2881 agcgctgtga tcgacttgcc acaccctgat tcgcctacaa gcccgagtaa ttcaccacca
     2941 tcgaggtgga agctcacgcc gtcgaccgcg cggacgagtc cagcgcgcgt ctggaattga
     3001 gtgcggaggt ttttgacttc gagtagatgt ggcacggcag gcgttgcgat ttcagtagag
     3061 gttaaaattg tattcaagct ggatgtgcgt cgacacgaac ctaccgtcct taatggcagg
     3121 gataaacttt atttggcgcg ccgcgtcgat ggctttttgg gtcaaaccat gtggtaagcc
     3181 gctcagggct cgaattttga tgacttcgcc agtttctgaa aacacagctc gcaggacaac
     3241 tgttcccgtt gtctgggtct gacgtccttc ctcggtgtag gtcggttcag gctttgacaa
     3301 gacccgcgcc ttttgagtca cttcgcgccc cacgaaaggt ggaggatcac cttctttggt
     3361 tatcgacagc gaatacagta attgcacctc gctggggcct tcgtggataa cattgccttc
     3421 cgttggactt ccgaaactga tcgattcaaa gaatttctgc aagttgagat ccggattaac
     3481 aagcgtcgaa ctcagagcgg agaatacata agtcctattc ttggtagcca ggaattg
//
LOCUS       DASUEX010000203         4749 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1282139, whole genome shotgun sequence.
ACCESSION   DASUEX010000203 DASUEX010000000
VERSION     DASUEX010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4749
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1282139"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="VFV61_03390"
     CDS             <1..495
                     /locus_tag="VFV61_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899546.1"
                     /translation="VPGEVPSSEPKTGPLGLPSPEEILRRRQDSRRNRDRLPPGDRPP
                     AGEIGVDLPTGRGDPRVSSRGREKAASEDPILVMLDRLYNTADAYLQSIVEKSGGRLL
                     RADSVTALPVAFSQVAAELRSQYLLGYDPSNKSRDDQYHVIKVTTTRPGLVVRARPGR
                     RQGK"
     gene            675..1250
                     /locus_tag="VFV61_03395"
     CDS             675..1250
                     /locus_tag="VFV61_03395"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HEX4899547.1"
                     /translation="MPDRSAAWELLCEYTKGDSLRKHALAVEAAMRAAAQRYGEPEAD
                     VDEWGLVGLLHDFDYEMFPTPDQHPFTGANILCGRGYSERIIRAIMGHATYTGVPRDT
                     QMARALFATDELCGFLVACALVRPDKSLAGLEVKSVRKKLKDKAFARSVNRDDIRQGA
                     EELGVDLDEHIRFVIDALLPLQEEIGLRAAH"
     gene            complement(1256..1594)
                     /locus_tag="VFV61_03400"
     CDS             complement(1256..1594)
                     /locus_tag="VFV61_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899548.1"
                     /translation="MRLKLVMLVAVIAALVGAGSSVAIILTRSTSVHVLSSPDLVVTA
                     TLVLPIACVILAAFFVYRHTARRRKTQALLTGVLAAFLSLGLFILASIVTSRNIPRPP
                     QQQPTPRNVG"
     gene            1716..2636
                     /locus_tag="VFV61_03405"
     CDS             1716..2636
                     /locus_tag="VFV61_03405"
                     /inference="COORDINATES: protein motif:HMM:NF013761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HEX4899549.1"
                     /translation="MLTRSALIYLSQKEGLKDFAARFRLFKKLTTRFIAGESIAEAVA
                     AIRELNGEGCSASFDHLNESVANAAEAEGEVREYLRVLEQIDETGINSNISIKLTQFG
                     LGLDPELAYRNARAVVAEAARRGHFVRVDMESSEVTQATIDVFKRLRSEFGLNDVGIV
                     LQSYLRRTYDDAVELLKLPARIRICKGAYDEPPEVAFPDKQDTDNNYVRVMQLLLKSQ
                     VYHGIATHDPKMIEATLDFMRREGIAKDSFEFQMLYGIRRDLQRQLARDGYNMRVYVP
                     YGKHWYPYFMRRLAERPANIWFVLKNLLKG"
     gene            2641..3132
                     /locus_tag="VFV61_03410"
     CDS             2641..3132
                     /locus_tag="VFV61_03410"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEX4899550.1"
                     /translation="MENLTWILWCVLGLVLIVAEVFTTGFVLLWFGIGALAAAFVGLI
                     GIDSLAIQFLIFILVSGGLTAASRTIFVNYFSRDKTGPSLRSGADALPGKIGTVVSSS
                     RGALNEGAVKVYGSTWTAYPAAGESPLEAGERVRVESIEGASIYVRRVDDGRGWQQKS
                     LDE"
     gene            complement(3247..4170)
                     /locus_tag="VFV61_03415"
     CDS             complement(3247..4170)
                     /locus_tag="VFV61_03415"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HEX4899551.1"
                     /translation="MIVEVHAKSDVGRVRRGNEDNFLVLDLSSQKTWTGSDGNKPPDE
                     INKLQVGDKGLVLVVSDGMGGALAGDVASRMAVDSVREMLIGVNSEDGCAVETPLVDC
                     LKSATVYANLAIHKRSQEDSRCAGMGATFTGAAVKGDMLDLVQVGDSRGYMIRRDQIR
                     LATKDQSLVQQLVDVGQISEAEAETHMFRNVILQALGAQSEIIPVTGKIQLRRGDMLL
                     LCSDGLSGKLRSEDIQNIIAGNPDLATACDELVAEANNRGGEDNITVVLVRFLGDDLQ
                     EPTGDHITVELPPLEEDKTLDDTFEADTEAS"
     gene            complement(4248..>4749)
                     /locus_tag="VFV61_03420"
     CDS             complement(4248..>4749)
                     /locus_tag="VFV61_03420"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEX4899552.1"
                     /translation="EDANWIGFIQNGAGAILSPFDSWLVMRGTKTLALRMEQHDKTGR
                     AVAAFLEEHPHVQKVYYPGSASHPQHALARRQQKGFGGMVAFDVGSLEAARTVLESVK
                     LCTLAESLGGVETLISHPATMTHASVNAEKRERLGITDGLVRISVGLEDTDDIIADLD
                     QALKQL"
BASE COUNT         1088 a         1294 c         1300 g         1067 t
ORIGIN      
        1 gttcctggag aggtcccctc atcagaaccg aagactggac cgctcggttt accatcgccg
       61 gaggagattc tgagaagacg acaagattca cgtcgcaacc gagatcggct gccgcctggg
      121 gatcgtccgc cggcaggcga aatcggagtg gatctgccca ccggccgcgg tgatccacgc
      181 gtttcctcca gaggtaggga gaaggcggca tctgaagatc cgatcctggt gatgttggat
      241 aggctttaca acaccgccga cgcttacctg caaagcatag tcgaaaagtc gggcggacgg
      301 ctcttgcgcg cagattcagt tactgctctt cctgtggcct tttcccaagt cgccgccgag
      361 ctgcgctccc aatacctcct tggctacgat ccctcgaata agagtcgcga cgaccaatac
      421 cacgtaataa aagtgaccac cacgcgtccc ggactagtcg tccgcgctcg ccctgggcgc
      481 cggcaaggga agtgaaaggc gattggcgat tggcaatttc cgattgccaa ttggccctta
      541 acgaaaaggg caatcgaaga tccgcaatcg gcaatcggcc agcgccaatt cgatcacctt
      601 cttgtttagc tccgctatcc cgtgctagtt taaatcgcta cacacaaacc aacttccagt
      661 cgttcactgc aagcatgcct gatcgaagcg ccgcgtggga attactatgc gaatatacaa
      721 agggcgatag cttgcggaag cacgctctcg cagttgaagc tgccatgcgt gccgccgccc
      781 aacgctacgg tgaacctgag gctgacgtcg atgagtgggg acttgtgggt ctgctccacg
      841 atttcgacta cgaaatgttt ccgactccgg atcagcaccc atttacgggc gcgaatatcc
      901 tttgcggccg cggctattcc gaacgaatca ttcgggcgat tatgggccac gccacctaca
      961 caggcgtgcc gcgcgacaca cagatggccc gcgccctatt tgccaccgat gaactgtgcg
     1021 ggttcctggt cgcttgcgcc ctggtgcggc ccgataagag cctcgctggc ctggaagtga
     1081 agtcagtgag gaagaaacta aaagataaag cctttgcgcg atctgtgaat cgagatgaca
     1141 ttaggcaggg cgcagaggaa cttggcgtcg atttggatga gcatattcga ttcgtaatcg
     1201 acgccttgct gccgctacaa gaagagattg gattgcgcgc ggctcattag agcactcaac
     1261 cgacattgcg tggcgttggt tgttgctgtg gcggtctggg aatatttcgc gaagtaacaa
     1321 ttgatgcgag tatgaagagt cccagactga ggaatgccgc taacaccccc gtcagcagcg
     1381 cctgagtttt ccgccggcga gcagtatgcc gataaacgaa aaaggcggcg agaatcacgc
     1441 aagcaatggg tagcacgaga gtcgcggtta ctaccagatc cgggctcgac aggacatgca
     1501 cagaagtcga gcgcgtcaga atgatggcga ctgaggagcc ggcacccact agtgccgcaa
     1561 tcaccgcgac caacatgacc agcttcaatc gcattgaaac caagccctca aaagggattt
     1621 aatccgtcga ggaggccaag aataccatag cctcaaagtc ctggcccggg tggcccattt
     1681 caagcaggtg acaaaggaca gccaacttcc cagacatgct caccagaagc gctttaatct
     1741 acttatcgca aaaagagggt ttgaaagact tcgccgcccg ctttcgtttg ttcaagaaac
     1801 taacgacgcg ttttattgcg ggtgaatcaa tcgccgaggc ggtggcagct attcgcgagc
     1861 taaacggtga agggtgcagc gcttctttcg atcatttgaa cgagtcggta gctaatgccg
     1921 cagaggccga aggggaagtg cgcgagtacc tgcgagttct cgagcaaatc gacgagacgg
     1981 gaatcaactc gaacatttcg atcaaattga cacagtttgg tctggggctt gaccccgaac
     2041 tggcgtaccg gaatgctcgc gcggtagttg cggaagctgc gcgccgcggc cacttcgttc
     2101 gcgttgatat ggagagctcc gaggttaccc aggcaactat cgatgtcttc aagcgactgc
     2161 gatctgaatt tggactgaac gacgtgggta tcgtgctgca atcatacctg cgacgaacct
     2221 atgacgatgc agtagagttg ctgaaactcc cggcacggat tcgcatctgc aaaggtgctt
     2281 atgatgaacc gccggaagtt gcttttcccg ataagcagga cacggataac aactatgtcc
     2341 gggtgatgca gctattactt aagagtcagg tctatcatgg aatcgctact cacgatccta
     2401 aaatgattga ggctacgctc gatttcatga ggcgcgaagg tattgcgaaa gactcttttg
     2461 aatttcagat gctctatggt atccggcgcg acttgcaacg ccagctggcc cgcgacggtt
     2521 acaacatgcg agtttacgtt ccttatggaa aacactggta tccttacttc atgagacgcc
     2581 tggcagaacg tccggcaaat atctggtttg tcctgaagaa tctgctgaag ggctaagcga
     2641 gtggaaaatc ttacctggat cctctggtgc gttctcggcc tggtgctgat tgtcgccgaa
     2701 gttttcacta ccggatttgt tttgctttgg tttggaatcg gcgcgctcgc ggctgccttc
     2761 gtcgggctga taggcatcga tagcctggcg attcaattcc taatcttcat cctcgtgtcc
     2821 ggtggcctca ctgccgcatc gcgaacaatc ttcgttaatt atttttcgcg cgataagacc
     2881 ggcccctcgt tacgcagtgg cgcggatgca cttcctggca agattggcac cgtagtttca
     2941 tccagccgcg gtgcactcaa tgaaggagca gtaaaagttt atggttcgac ctggacggcg
     3001 tacccggctg cgggcgaatc gccattggag gcgggggagc gcgttcgagt tgaaagcata
     3061 gagggcgctt cgatctatgt caggcgagta gacgatggcc gcggctggca acaaaaatcg
     3121 ctggacgagt aaggcgattc atgttgcagt gcgctgaatc gtccgcgctc taagctggct
     3181 tatacaaaaa aagcggcaac ccgacacgtc gagggccgct cctcccagaa caagctattt
     3241 gagaatttaa gaagcttcgg tgtccgcttc gaacgtgtcg tcgagggtct tatcttcttc
     3301 gagcgggggc agctcaaccg taatgtgatc cccggttggt tcttgcaagt cgtcgccaag
     3361 aaaacgaacc aggacaactg ttatgttatc ttcgccgccg cgattgtttg cctcggccac
     3421 caattcgtcg caagcagtcg cgagatcggg gtttccggcg atgatgttct gaatatcttc
     3481 tgaacgcagt ttccccgaaa gtccatcgct gcacaacaac aacatatcac cgcgacgcaa
     3541 ttgaatcttt ccagtaacag gaatgatttc actttgagcg cccagcgcct gcagaatcac
     3601 gttgcggaac atgtgggttt cagcttctgc ttcactgatc tggcccacat caaccaactg
     3661 ctgcaccagg gattgatcct tggttgccag ccgaatctga tcgcggcgaa tcatgtagcc
     3721 acgactgtcg cctacctgca ccagatccag catatcgccc ttgactgccg cgcctgtgaa
     3781 tgttgccccc atgccggcac agcgcgagtc ttcctgactg cgcttgtgga tcgccagatt
     3841 cgcatacact gtcgcgctct tgaggcaatc gacgagggga gtttccacgg cacacccgtc
     3901 ttcagagttg acgccaatca acatctcgcg gactgagtcc accgccatgc ggctggcaac
     3961 atcacctgca agggctccgc ccataccatc agaaacgacg agcacaagac ctttatcgcc
     4021 gacctggagt ttattgattt cgtctggagg tttattgccg tcagagccgg tccaggtttt
     4081 ctgactggag agatccagaa ccagaaaatt gtcttcgttc ccacggcgca cccgaccgac
     4141 atcagacttt gcgtgaactt ctacaatcat aaggagagcg gagtgcgatg tactattggc
     4201 tcgaagattt taacccttct gcagcgaaac tgtcacttgt tcagttttta tagttgtttt
     4261 agagcctggt caagatcggc aatgatgtca tccgtgtctt ccagtccgac tgagatgcgg
     4321 accagcccat cagtaattcc gagccgttct cgtttctctg cgttcacaga tgcgtgagtc
     4381 attgtcgccg gatgagagat cagtgtttct actccgccca gactttccgc gagcgtgcac
     4441 agcttaacag attccagcac ggtccgcgcc gcttcgagcg agccaacatc gaaggcgacc
     4501 atacccccaa aacctttttg ctggcgtcgc gcaagcgcat gttggggatg cgacgcggag
     4561 ccgggatagt aaaccttttg gacgtgggga tgttcttcga ggaacgccgc aaccgcgcgg
     4621 ccggtcttgt cgtgctgttc catgcgtaac gcgagggttt tggtaccacg catcaccagc
     4681 caggaatcaa aaggcgaaag aatcgcgccc gcgccgtttt ggatgaatcc gatccagtta
     4741 gcgtcttct
//
LOCUS       DASUEX010000204         2926 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1627587, whole genome shotgun sequence.
ACCESSION   DASUEX010000204 DASUEX010000000
VERSION     DASUEX010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2926
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1627587"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="VFV61_03425"
     CDS             <1..243
                     /locus_tag="VFV61_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899553.1"
                     /translation="LVEGGRHFVEPVEALIRGSSIHGSMLGRGWIGIGLRIEMWVEGK
                     LASTSPVKSIHVRRHTRAGAAVVINSQAGADNQRRI"
     gene            243..1307
                     /locus_tag="VFV61_03430"
     CDS             243..1307
                     /locus_tag="VFV61_03430"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX4899554.1"
                     /translation="MEKMQANVFYGKNDIRVEEVEKPRARAGEAVMRITLTTICGTDL
                     HIVRGEYPVASGLVIGHEPVGVITELGEGVTGYEIGDRVLVGAITPCGQCRACLSGHA
                     SQCGHGGDYEALGGWRFGNTINGAQAEYLRVPHAQANLAKIPKELTDEQVVLLADIAS
                     TGFGGAEAARIKIGDAVVVFAQGPIGLCATAGAKLMGASFIIGVDGDENRLAMSKRMG
                     ADVTLDYRHVDVVAEVKRLTGGGADVAIEALGTQPTFESALRCLRPAGTLSSLGVYSG
                     KLEIPYEAFAAGIGDHKIATTLCPGGKERMRRLMEVVRYGRVDFTPLLTHRFKLADIP
                     AAYELFGERADGVLKVAIQP"
     gene            1336..1827
                     /locus_tag="VFV61_03435"
     CDS             1336..1827
                     /locus_tag="VFV61_03435"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HEX4899555.1"
                     /translation="MKIEEVFDQPGEITRRTFCNRVVMTSIGLGLAAKSLSGQTAAAQ
                     TPAVAYPPRKIEGAEQVLPGTSLYFNYPRRNDPAVLVRAQDGEYFAYSRKCAHLGCSV
                     EFDSQRRCLKCPCHQGAYDARTGYVMYGPPPRPLDQIMLQMRAGGEVWAVGKTPVSAS
                     HDT"
     gene            1906..2397
                     /locus_tag="VFV61_03440"
     CDS             1906..2397
                     /locus_tag="VFV61_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899556.1"
                     /translation="MSRNLISNTLSRVLIAALFALGMSFGAHANTQTVKATIKPNAET
                     QQPRYTEYRGVKLGMTTDEARAKLGTPSMKYSDQDFYMVSENETAQIIYDAAHKVIAI
                     SVDYLGGIGAPDYKTVVGVELQTTANGSLYRVVRYESQGFWVSYHRSQGSVQTVTVTI
                     QKI"
     gene            2515..>2926
                     /locus_tag="VFV61_03445"
     CDS             2515..>2926
                     /locus_tag="VFV61_03445"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX4899557.1"
                     /translation="MNEPIKILIAYDGSECAQAALEDLKIAGLPKKAEVFVISLADVI
                     LPPGHEEKSNSSPSHLPPGVRRAYERAELKLKHAESLAKEAGDQVKKSFPEWQVRFEA
                     LADSPAWAIIRTAEDWKPDLIVMGAQGHAVLGGRL"
BASE COUNT          683 a          746 c          889 g          608 t
ORIGIN      
        1 ctggtggagg gcggacgtca ctttgtcgag ccagtcgagg cgctgatccg gggttcgagt
       61 attcacggtt ccatgcttgg tcgcggatgg ataggaatag gactgcgcat agagatgtgg
      121 gtggagggca aactagcgag cacatcgcca gtcaagtcaa tccacgtccg gcggcacacg
      181 cgagctgggg cggcggtggt tattaactcg caagccggcg ccgataatca gcggagaatc
      241 tgatggaaaa aatgcaggca aatgtttttt acgggaagaa cgacatcagg gtagaggagg
      301 ttgagaaacc acgagctcgc gccggcgagg ctgtcatgcg cataacgctg accacgattt
      361 gtggaacgga tctgcacatc gtgcgcggcg agtatccagt ggcgtcaggt ctcgtaatcg
      421 gtcacgagcc ggtgggcgtg attacagagc ttggtgaggg agtcacagga tacgagatag
      481 gcgaccgtgt gctcgtcggc gcgatcactc cgtgcggtca gtgccgcgcc tgtttgtcag
      541 gacacgcttc ccagtgtggg catggcgggg attacgaagc ccttggcggt tggcgtttcg
      601 gtaacaccat caacggtgcc caggctgagt atcttcgcgt tccccacgcg caggccaacc
      661 tggcaaagat cccgaaagag ttgactgacg aacaagtagt cttactcgcc gatatcgcgt
      721 ccaccgggtt cggaggcgcc gaggccgcca gaataaaaat cggtgacgcg gtggttgtct
      781 ttgcgcaggg accaatcggc ttatgtgcca ccgccggtgc caaacttatg ggagcgtcgt
      841 tcatcatcgg tgttgacggt gatgagaatc gacttgctat gtcgaaacgc atgggcgcgg
      901 acgtaacact cgactatcgc cacgttgacg ttgttgcaga ggtaaaacgt ttgaccggag
      961 gcggggctga tgtggcaatc gaggcgctgg gaacgcagcc aacattcgag agcgcgttac
     1021 gctgcctgcg gccggcagga acactgtcga gtctcggcgt ttattccggc aagttggaaa
     1081 ttccgtatga agcattcgcg gcgggcattg gcgatcacaa gatcgccacc acgctctgtc
     1141 cgggcggaaa ggaacgtatg cgccggctaa tggaggttgt gcgttacggg cgtgttgatt
     1201 ttacacccct gctgacgcac cgcttcaaac tcgcggacat acccgcagcc tatgaactct
     1261 ttggagagcg agccgacgga gtgctgaaag tcgcaattca gccgtaagca gccaacgtgt
     1321 gaaaggagtg ctgcaatgaa aatcgaagaa gtctttgatc aacctggcga gatcacccgt
     1381 cgcacttttt gcaaccgagt agtgatgact tcgatcggcc tgggattggc ggcgaagagt
     1441 ctcagcggtc aaacagctgc agcacagact ccagcagtcg catatccacc ccgaaaaatc
     1501 gaaggcgccg aacaagtgct gcccggcacg tcactttatt tcaattatcc caggaggaat
     1561 gatccggcag tgctggtgcg tgctcaagat ggagagtatt tcgcctacag tcgcaaatgc
     1621 gcacacctgg gatgctcagt ggaatttgat tcacagcgtc gttgcttgaa gtgcccctgc
     1681 catcaggggg cctacgatgc gcggacgggt tatgtgatgt atggaccgcc gccgcgcccg
     1741 ctggaccaga tcatgttgca gatgcgcgcc ggcggcgaag tatgggccgt tggcaaaaca
     1801 cctgtgagtg ccagtcacga tacgtgacag cttgccggtg agttggcgct taaccatggc
     1861 cactcgcacc agccagcagt cttcgttagt taggaggtag gaaaaatgag tcgaaactta
     1921 atcagcaata ctctctcgcg ggtcttaatc gccgccttgt tcgctctggg tatgagtttt
     1981 ggcgcgcacg ccaacacaca aacggtaaaa gcgactatca agcccaatgc tgagacgcaa
     2041 cagccccgtt acacagaata ccgcggtgtg aaactgggta tgactacgga tgaggcacgc
     2101 gccaaactcg gcacaccgtc aatgaaatac agcgaccagg acttttacat ggtttctgaa
     2161 aacgaaactg cacagattat ctatgatgct gcgcacaaag taatcgcgat ttcagttgat
     2221 tatctgggtg gaatcggcgc gcccgattac aaaacggtag tgggtgttga gctgcaaaca
     2281 acggccaacg gttcgctcta tcgagtggtg cgttacgaga gtcagggctt ttgggtctcg
     2341 tatcaccgat cgcagggatc ggtgcaaacg gtcactgtca ccattcagaa gatctgatcg
     2401 ctggtgagta gattagcagg actgaattga tcctagctta ggtttcttgg gcaaagctgt
     2461 acagcgccaa caccgccccc tgcagatcgg tgcggattga actacaatgc gcgcatgaac
     2521 gaaccgataa agatactcat tgcttacgat ggctctgagt gcgcgcaggc tgcactcgaa
     2581 gatctcaaaa tcgccggact gcccaaaaaa gccgaagtat tcgttattag tctggccgat
     2641 gtgattctgc caccgggcca tgaagagaaa agcaactcat ctccttcgca cctaccgccg
     2701 ggtgtgcgac gtgcttacga acgggccgag ctcaaactga agcatgcaga gtcgttggca
     2761 aaagaggcgg gcgaccaggt taagaagagt tttcccgagt ggcaggttcg cttcgaagca
     2821 ctcgctgatt caccggcctg ggcaatcatc cggaccgctg aggattggaa gcctgacttg
     2881 attgtcatgg gcgcgcaggg acatgcggtg cttggtggca ggctga
//
LOCUS       DASUEX010000205         2050 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1282840, whole genome shotgun sequence.
ACCESSION   DASUEX010000205 DASUEX010000000
VERSION     DASUEX010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1282840"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1321)
                     /locus_tag="VFV61_03450"
     CDS             complement(<1..1321)
                     /locus_tag="VFV61_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899558.1"
                     /translation="MLTKTLITAGVMIAALLSASSGNASRGLLQHSRALSHVSVSDDY
                     QAVPDQREIRQEFHQTYPFSPTGRVSVENLNGEVRIAVWDRNDVQVDAVKRAYKQERI
                     DEAKIEVNAGAEAIRIRTDYPDWDQRFTDNERGRTNNPAIVDYSITVPRKARLESIDL
                     VNGSLEINGVEGDVKASSVNGRVIARGLLGIAKLSTVNGGLEATFANLDSARPVSLNS
                     VNGPVTLIIPSDANAVVRAGTVHGSISNDFGIQVHHGDYVGHELYGQIGQGGPTIKLG
                     NVNGPIKIKHAQDGRALSPATGLIPDKEKKNKGYGESEDGDDDARLSAEERREAAAAR
                     RAERAEQANAERMSRETQAEVQREVERAIREAQREIERAQREVERETRQQIRTQTRQE
                     IREARGVGRGEGEGDGKEFTEKESKTFTVSGTPSVNLSTFDGAITIHG"
     gene            complement(1401..>2050)
                     /locus_tag="VFV61_03455"
     CDS             complement(1401..>2050)
                     /locus_tag="VFV61_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899559.1"
                     /translation="LQEVAGNLQSATASSLGGLAKVYSATKSLAQGTELAQSPQVTLS
                     EPPRMDVIAAGGSVTLASFASGSAAGFSSNKSLAIEKVHSKLACSLRKRDLPKAADKD
                     LNWEVVAQAPKRMELLRHEFPIKHNAEIAQLLRREPKLRAIISKLPIKAGKGDWSNVG
                     EFRSLSDVIGFGLKAAQAEEAEIQKVETTEDATRHVFEFKRAVSDSEKDEMTSQE"
BASE COUNT          386 a          625 c          518 g          521 t
ORIGIN      
        1 agccgtgaat agttatcgcg ccatcgaagg tcgagagatt tacactgggc gtgccgctga
       61 cggtaaaagt cttcgactct ttttcagtga actctttgcc atcgccttcc ccttcgccgc
      121 ggcctacacc tcgtgcttca cgaatttctt gacgcgtctg ggtgcggatc tgctggcggg
      181 tttcgcgttc aacctctcgc tgggcccgct cgatttcacg ctgagcttcg cgaatggcac
      241 gctccacttc cctttgcact tcagcttgag tttcacgcga cattctctcg gcgttggcct
      301 gttcagcacg ttcggcccgg cgcgccgcag cggcctcgcg ccgctcttcc gcgctcagac
      361 gcgcatcgtc atcgccgtct tcactttcgc cgtagccttt gttcttcttc tctttgtcgg
      421 gaattagtcc cgtcgccggg ctcaaggctc ggccatcctg ggcatgcttg atcttgatag
      481 gaccgttaac attgcctaac ttgatcgtgg gtccgccctg gccaatctgg ccgtagagct
      541 catgaccaac gtagtctccg tgatgcacct gaatcccgaa atcattgctg attgagccat
      601 gaacggtgcc cgcccgtacg acggcattcg catccgaggg aatgatcagt gtcaccgggc
      661 cattaactga gttcagtgaa actgggcgag cactgtcgag attggcaaag gtcgcttcca
      721 gaccaccgtt aactgtggaa agcttggcta ttccgagcag accgcgcgca atcacccggc
      781 catttactga cgacgccttg acgtctccct cgacgccgtt tatttccagc gagccattga
      841 cgagatcgat cgactccagc cgagccttgc gcggcacggt gatggaatag tcaacgatgg
      901 ccggattatt tgtgcgaccg cgctcgttat cggtgaagcg ctggtcccaa tcgggatagt
      961 cagtgcgaat ccgaatcgct tcggcgcccg cgttcacttc gatcttcgct tcgtcgatac
     1021 gctcttgttt gtaagcgcgc ttaacagcat ccacctgaac gtcatttcga tcccaaactg
     1081 caatacgaac ctcgccattt agattttcga cacttactcg gcctgtgggt gaaaacggat
     1141 aggtctgatg aaactcctga cgaatctctc gctgatccgg taccgcctga tagtcgtcac
     1201 taaccgacac gtgcgacaac gcacgtgaat gttgcagcaa accgcgcgag gcgttgccgc
     1261 tcgaggcgct caacagcgcc gcaatcatta ctcctgccgt tattaaagtt ttcgttaaca
     1321 ttctgcttct cacttttact ccatttgtta ttacgctgag gcgcaaaccg ttgcgcctga
     1381 tttgccgccc gggctcgttc ttattcctga gacgtcatct cgtctttttc actatcgctg
     1441 acagcgcgtt tgaattcgaa aacatgacgc gtcgcgtctt cggtggtctc gaccttctgg
     1501 atttccgcct cttcggcctg cgctgccttt aatccaaaac cgattacgtc gcttagagat
     1561 ctaaactcgc caacgtttga ccaatcaccc ttgcccgcct tgatcggcag cttgctgatg
     1621 atggcgcgca acttgggttc ccgacggagc agttgtgcta tctcagcatt gtgcttgatc
     1681 gggaattcgt ggcgcagcaa ctccattcgt ttgggggctt gggcgacaac ctcccagttc
     1741 agatccttgt ccgccgcctt tggtaaatct cgcttgcgca gcgaacaggc aagcttgctg
     1801 tggacctttt caatcgcgag cgatttgttg ctactgaacc cagcggcgga accactggcg
     1861 aaacttgcga gcgtcactga acctcccgcg gcaatgacgt ccatgcgcgg aggctccgac
     1921 aaagtcactt gcggcgactg agctaattca gttccctggg ccagagattt cgtggctgag
     1981 tagaccttcg ccaatcctcc caaactactg gcagtcgcgg attgcaaatt gcccgccact
     2041 tcctgcaacc
//
LOCUS       DASUEX010000206         2526 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1531956, whole genome shotgun sequence.
ACCESSION   DASUEX010000206 DASUEX010000000
VERSION     DASUEX010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2526
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1531956"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="VFV61_03460"
     CDS             <1..236
                     /locus_tag="VFV61_03460"
                     /inference="COORDINATES: protein motif:HMM:NF020977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEX4899560.1"
                     /translation="ELAAKEQAEIEVIETYLPKAASQDEIDQAVAAAIAETSAASLKD
                     MGKVMKAAQAALAGKNADGRAVSETVKAKLGAL"
     gene            707..1294
                     /locus_tag="VFV61_03465"
     CDS             707..1294
                     /locus_tag="VFV61_03465"
                     /inference="COORDINATES: protein motif:HMM:NF022215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2585 family protein"
                     /protein_id="HEX4899561.1"
                     /translation="MSETPKLNALISRLAPSLFILSVLGLALFQLRNQGRSWWCACQQ
                     YFPWAGDIWSAHNSQHLLDPYSFTHLLHGFAFCWLLALAIPRLSSTWRLALATLLEAA
                     WEVFENTEFVIRRYREETAALGYNGDSVFNSFGDILLCIAGFMIAQRLGFKRSVLVFV
                     ATELVLLLWIRDSLLLEILMLVYPIEGLKAWQIGK"
     gene            1640..2137
                     /gene="rimP"
                     /locus_tag="VFV61_03470"
     CDS             1640..2137
                     /gene="rimP"
                     /locus_tag="VFV61_03470"
                     /inference="COORDINATES: protein motif:HMM:NF014617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEX4899562.1"
                     /translation="MSPSSTEERVKAIAEKVAIDQGLELVHAEVAGPENKPIVRIFID
                     KPAGVTHQDCSEVSLHLGTILDVEDFIHSSYTLEVSSPGLDRGLYKRADYERFAGSLA
                     KVKTKRPVGNQRNFRGRLLGVEGDDVLFDDKTSGRVRIALDAIVKANLEIDFDAELRK
                     PITRL"
     gene            2204..>2526
                     /locus_tag="VFV61_03475"
     CDS             2204..>2526
                     /locus_tag="VFV61_03475"
                     /inference="COORDINATES: protein motif:HMM:NF020118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NusA N-terminal domain-containing protein"
                     /protein_id="HEX4899563.1"
                     /translation="MSSSIAQNIEALCQEQGIERDLVIEAMKEAVRAAAKKQFRSGED
                     IQVDWNPETGIELSASKVVVEEVENGATELSIDEARELAGDEVELGDALLLPLPMEEL
                     GRIAA"
BASE COUNT          573 a          676 c          678 g          599 t
ORIGIN      
        1 cggagttggc tgcgaaagag caggcggaga tcgaagtgat cgaaacctac ttgcccaagg
       61 cagcgtccca ggacgaaatc gatcaggctg tcgctgccgc gattgcagaa acgagtgcgg
      121 cgtccctgaa agacatgggc aaggttatga aggccgcgca agcagccctc gctggcaaga
      181 acgccgatgg tcgcgctgtg agtgagactg taaaggctaa actcggtgcc ttgtagcgga
      241 acagtttcat tcacaccgca ctttagggcg gtgtcgctag agaaccgaat tagaaaccat
      301 ttaaacggca atgatatatt tgactcacca agccatgtca tcgtgtttgg gttccaaaag
      361 cgccgcaggc gcgacattca atagccccgc ggccgtaagg ccgtggatcc gaaggcctca
      421 agatgaaatc gaggcccgca gggccgacat tcctctgcac cgtgatatgg ggtttacggc
      481 cgcaccttcg gcgctcgact atttaggttt cgactgacca cggcctcacg gccgtggcta
      541 tttgatgacg gtcctgcgga cctcacttgg cactctcaga cgtgtgtttg atttcgccga
      601 aattgagtca aatatatcat tgccgtttca acggtttccc tttctaaccc tcggactatc
      661 actgggctag agcccggtga gaatgaaagt ctcaagtcaa aatcagttgt ccgaaacacc
      721 taagcttaac gcgctaattt ccaggctcgc gccttcgctc ttcatcctct ccgttctggg
      781 tctcgcactt tttcagcttc gcaatcaggg ccgttcctgg tggtgtgcct gccaacagta
      841 cttcccctgg gccggcgaca tctggagtgc tcataactcg cagcacctac tcgatcctta
      901 ttctttcacg cacctactcc atggctttgc attctgctgg ctgttggcac tcgccattcc
      961 acgattgtca tcaacgtggc gcctggcgtt ggcaaccctt ctggaagctg catgggaggt
     1021 atttgaaaac accgaattcg ttatccgacg atatcgggaa gagaccgccg cactgggcta
     1081 caacggcgat tctgttttta attcatttgg cgacattctg ctttgcatcg ccggcttcat
     1141 gatcgcccag cggctcggct tcaaacgcag cgtgctggtg ttcgttgcta ccgagttggt
     1201 gcttctactc tggatcagag acagcctgtt gctggagatc ctgatgcttg tctatcccat
     1261 cgaggggctt aaggcttggc agataggaaa gtaacgcccg gggcatcgga ctgacccgaa
     1321 acctcttaaa ttccattttc cagttctcat ttctcatttt tcattgtcag cctcttttta
     1381 accccatttt cgtagggtcc cagacctgaa gaatcgaaat cgaatgaaaa atgagaattg
     1441 agaaatgaat gaatggaaaa tgatcttgcc ttgaagttgc gctttacctg tttctgacca
     1501 tcgcaaaggc ttagttggcc ctcgcttgct tcccctcaac cctttccgta taatcctccg
     1561 cttgctcgaa gccgtcggtt ctgtcctgtg ggttcgcgcc cactttttct tttggggcat
     1621 gtagttttgg aggtacgggg tgagccccag ttcgactgaa gagcgcgtca aggcaattgc
     1681 ggaaaaggtg gctatcgatc agggtctgga gctagtccac gctgaagttg ccggccccga
     1741 gaacaaacca attgttcgca tcttcattga taagccggct ggagttaccc atcaggattg
     1801 ctccgaagtg agtctacacc tcggtacgat cctcgatgtt gaagacttca tccactcttc
     1861 ctacacgctg gaagtttcgt cgcccgggtt agatcgcggt ctttacaaac gggccgatta
     1921 cgagcgtttt gccggcagcc tggcaaaagt taagacaaaa cggccggttg gcaaccaacg
     1981 gaatttccgc ggccgtttgc tgggagtcga gggcgacgac gtgctctttg acgacaaaac
     2041 aagtggtcgc gtcagaattg ctctggacgc gattgtgaag gcaaatctgg aaattgattt
     2101 cgacgcggag ttgcgaaaac cgataacgcg tctgtaacaa acaactgaat aggctcagcg
     2161 acgcagaagc gattcgggcg tcgagccacc cgggagactt taagtgagtt catccatagc
     2221 acaaaacatc gaggcccttt gccaggagca ggggatcgaa cgcgacctcg tcatcgaagc
     2281 catgaaggag gcagtccgcg cggctgccaa gaaacagttt cgctccggcg aagacattca
     2341 ggtcgactgg aatccggaaa ccggcatcga gctctcggct tccaaggttg tcgtcgaaga
     2401 ggtagagaac ggcgccaccg agctttcgat cgacgaagca cgcgagctag cgggcgatga
     2461 ggttgaattg ggagacgcgc tgcttctacc actgccaatg gaagaactag gacgcatcgc
     2521 cgcaca
//
LOCUS       DASUEX010000207         2448 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1628941, whole genome shotgun sequence.
ACCESSION   DASUEX010000207 DASUEX010000000
VERSION     DASUEX010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1628941"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /locus_tag="VFV61_03480"
     CDS             <1..490
                     /locus_tag="VFV61_03480"
                     /inference="COORDINATES: protein motif:HMM:NF016184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein tyrosine phosphatase family protein"
                     /protein_id="HEX4899564.1"
                     /translation="LLLSSLIVVRAQEVPPIRNFVRLNDDFCTGAQPRLEHLEKLKAD
                     GVKSILNLRPAGEHRAAEEEAKVKELGLQYFNIPVVFGEPKEEQVAEFFRITDERKNF
                     PIFIHCAAGIRVGAFWFIRRALRDGWTLEAAEKEADQIGLRESPHWKEFARKYIEQHR
                     QK"
     gene            593..2290
                     /locus_tag="VFV61_03485"
     CDS             593..2290
                     /locus_tag="VFV61_03485"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX4899565.1"
                     /translation="MSKSTLAQDQRFELPNYKLVLGVLVLQFYSGGTFSIEGKGWPTV
                     NGGWKLQGAEIELTPVGSGGCEVPGRYRLRHEGKGLGFELVSDECQLRKVILNGSNWR
                     PADEPKTIPLRRISHTATARPAKADWKSANGSWPSFRGAQAAGVADGQNLPDTWNAKT
                     GENILWRTPIPGLAHSSPVVWGNRVFVTSAVSSDPKASFRPGLYGDGDASQDRSRHRW
                     MMYALDKRDGKILWERVAYEGEPREKRHIKSTYANSTPATDGRIVVAWFGSQGVYAFD
                     VTGRPLWKVDLGRVDMGAYDIPTYEWGPASSPIIWNNLVILQCDTQADSFLLALDAGT
                     GKTVWKTEREELPSWGTPTVAMTGAGPVLVTNASNFIRGYDPRTGKELWRLGRSSKIT
                     APTPIFSDDMFVVASGRGPERPIFVVRANARGDLTLPDGKSDSESIVWSRTGRGSYMP
                     TPLSYKGQLYVLSNNGLFDAYNLKTGAEVYRQRLPLIGSGFSASPVAADGKIYLSNED
                     GEILVIAAGDKFAHVATNSMGELLMATPALSEGVMYVRSAQSISAVGRKQAAIKAKD"
BASE COUNT          543 a          640 c          713 g          552 t
ORIGIN      
        1 actgctcctg tcttcgctga ttgttgtgcg cgctcaagaa gtcccgccaa tcagaaactt
       61 cgttcgtctc aacgacgact tttgcaccgg cgctcaacct cgtctcgaac acctcgagaa
      121 gttaaaggct gacggtgtga agtcgatcct gaacctgcgg ccggctggtg aacatcgcgc
      181 ggccgaggag gaagcgaagg tcaaagagct cggcttgcag tatttcaaca tcccggtggt
      241 ttttggcgag cctaaagaag aacaagtagc cgagtttttc agaatcaccg atgagcgaaa
      301 aaactttccc atcttcattc actgtgcggc cggcattcgc gtcggtgcct tctggtttat
      361 ccggcgggca ctgcgcgatg gctggactct ggaagcggca gaaaaggaag ctgatcaaat
      421 cggcctgcgc gagagtccgc actggaaaga gtttgcgcgg aagtatattg agcagcaccg
      481 ccagaaatga ttcagggcct cagctgtccc cttaggagtc gatatgaaaa caggtagcag
      541 tagatccaga ttcttctctc ttctgatcac catagcgctc agcatgtctg taatgtccaa
      601 aagtacgctg gcgcaagatc agaggttcga gctgccaaac tacaagttgg tgttaggcgt
      661 gttggttctg cagttctatt cgggtggtac cttttcgatt gagggaaaag gctggcccac
      721 cgtgaatggc ggctggaaat tacagggggc tgagatagag cttacgcccg tcggatcggg
      781 gggttgtgag gtgccgggcc gttatcgcct gcgtcacgaa ggtaaaggct tgggattcga
      841 gctcgtgtct gacgaatgtc aactgcgcaa agtgattctt aatggcagca attggaggcc
      901 agcggatgag ccgaagacga ttccattgcg acgcatctcg cacactgcaa ctgcacgccc
      961 ggccaaggct gattggaaat cagcaaacgg aagctggcca tcgtttcgcg gcgcgcaggc
     1021 cgcaggcgta gccgacggtc agaacctgcc cgacacctgg aacgcgaaga caggtgagaa
     1081 tattctctgg cgcacaccaa ttcctgggtt ggcccactcg agccctgtgg tctggggaaa
     1141 tcgagttttt gttacgagcg cggtcagcag tgatcctaag gctagctttc gtcccgggct
     1201 ctacggagac ggcgatgcct ctcaagatcg gtcgcgtcac cgctggatga tgtatgcgct
     1261 cgacaagcgt gacggcaaaa ttctttggga gcgagtggct tacgaaggtg agccgcgtga
     1321 gaaacgccac atcaagtcga cttatgcgaa ttctactccc gcgactgacg ggcgcatcgt
     1381 tgtcgcctgg tttggctccc agggggttta cgcctttgac gtcacaggac ggccactgtg
     1441 gaaagttgat ttaggtcgtg tggatatggg ggcgtatgac attcccactt acgagtgggg
     1501 accggcgagc tcgcccatca tttggaacaa tctcgtcatt ctccagtgcg acacacaagc
     1561 tgattcattc ttgttggccc tcgatgctgg gacgggcaag acagtttgga aaacagaacg
     1621 cgaggagttg ccttcgtggg gcacgcccac ggtagcaatg accggagccg gcccagttct
     1681 tgtcaccaat gcttcgaact ttatccgtgg ttacgatccg cgcaccggca aagaactctg
     1741 gcgtctcggt cgcagttcta agatcacggc tcccactccg atcttctctg acgatatgtt
     1801 tgtggtggcc agtggacgtg gccccgaacg tccgatcttt gtagtcaggg cgaacgcgcg
     1861 tggggatcta actttgcctg acggaaaaag cgatagtgaa tccatcgtct ggagtcgcac
     1921 tggacgcggt tcttacatgc ccacgccgct tagttacaaa ggacagctgt acgtcctctc
     1981 taacaatgga ctcttcgatg cttacaactt aaaaactgga gctgaagtct accggcagcg
     2041 actaccgctc atcggcagtg gtttcagcgc ttcgcccgtc gccgccgacg gcaaaattta
     2101 tctttcaaac gaagacggtg aaattctggt gatcgcggcg ggagacaagt ttgcccatgt
     2161 cgccacgaac tcgatgggcg aactactgat ggcaactccg gcgctttcgg aaggcgtcat
     2221 gtacgtgcgt tcggcgcaaa gtatttccgc agttgggcgt aagcaagctg ctatcaaagc
     2281 gaaggactag gctatcgacg cggagcgtcg cggtgatttt agcccggctt ttcaaagccg
     2341 ggtagccacg ccacatttga ccggtcgcgt agcgacgatt cagattctag ccccataggt
     2401 aacgctcatc atattgaatc ccgtgttggc ggagtaactc cacgtatt
//
LOCUS       DASUEX010000208         3279 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_43247, whole genome shotgun sequence.
ACCESSION   DASUEX010000208 DASUEX010000000
VERSION     DASUEX010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3279
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43247"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="VFV61_03490"
     CDS             complement(<1..413)
                     /locus_tag="VFV61_03490"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX4899566.1"
                     /translation="MKFIETLKLAVAAIWAHKLRSVLTLLGMIIGVMAVVVVYSLIQG
                     FNTYVDEKIAGIGAKSFNVQRFNPLEDFKDTDTIAAAQRRNKDLTMEDFDYLKERATL
                     IGKLGARARGTPSEIKRGDQTLEDVFVSGATAAIA"
     gene            complement(624..1862)
                     /locus_tag="VFV61_03495"
     CDS             complement(624..1862)
                     /locus_tag="VFV61_03495"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX4899567.1"
                     /translation="MRLDDVKESSLMALDTLRSNKLRSSLTILGVSVGVVTIIFMVSI
                     IQGLNRAFADQIESLGSNTIFVSKFAPSFGRPPNPEEIHRKDLTMDDAEALREAPSIA
                     GVSPIHRMIAATARYQDKQTDTPILLGVTPYYEFVHSQYVASGRFIGEIDMQDRSNVC
                     VLGVDVMRALFPYENAVDKEIRINGNPYRVIGVMEPLGNFFGQSRDNSIFMPISTFDK
                     YYPDQPFPQVVFFIIVRPHSRAQVKSAMDEIADILRRRRRVPQNAPNNFGISSQDSLL
                     DVYNQLTGATALVLTAISFVALMIGGIGVMNIMLVSVTERTKEIGIRKAVGATKFNIL
                     SQFLIEAVVLTAIGGLAGLIVGEVASLLMNRYSPLPAYVPLWAVAMGIGISAAVGIVF
                     GLWPAWKAARLDPIEALRWE"
     gene            complement(1905..3260)
                     /locus_tag="VFV61_03500"
     CDS             complement(1905..3260)
                     /locus_tag="VFV61_03500"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX4899568.1"
                     /translation="MKRHQPDEIISNGMPNASRRDFALSRRSAGKSRFRLFRLTALWE
                     AFLVATASLRTNKLRTALTLMGIVVGVTAVIAVVTIIKGLDKTVAQTFSSQGSTVFTI
                     SKKPAIIKSREEFVTFNKRKDVTDEDADAIARLCTACWRVGIAANAAETVKHADQKAE
                     NVRIRGVTPLTMFEIDGVSIDAGRIWTEGEGDAGRPVAVAGADLLKNLFGDAPADRAV
                     GQEIRIAGRPYTVIGVLEPLGSIFGFSRDNVVYIPYSAYQQAFGARSSIVAFIQVPSA
                     EQLEAAEDQVRTIMLNRRGKMFSDDGDAGFSLETQDVFLDLYGQATSNIYIVTIGVAA
                     ISLVVGGIVVMNIMLVSVTERTKEIGIRKAIGARRKDILAQFLIEAVTVTAIGGALGV
                     ATGFGLAYIISMLIGFPLLVSVASAVLGVGVSSVVGIISGLWPAWRAAKLDPIEALRA
                     E"
BASE COUNT          775 a          901 c          881 g          722 t
ORIGIN      
        1 tctgcgatgg cggcggtggc gccggagaca aaaacgtcct cgagcgtttg gtcgccgcgc
       61 ttaatctcgg acggtgtgcc acgggcacgc gcgccgagtt ttccaatcag agttgcgcgc
      121 tctttcagat agtcgaaatc ctccatcgtc agatctttgt tgcggcgctg agctgcggcg
      181 attgtgtctg tatctttgaa gtcttccaga gggttgaagc gctgcacgtt gaacgacttg
      241 gcgccgatgc ccgcaatttt ttcatcgacg taggtattga atccctgtat cagcgagtaa
      301 acgaccacga cggccataac tcctataatc atgccgagca gagtcaatac ggagcgcagc
      361 ttgtgcgccc agattgcggc cacggcgagc ttcaacgtct cgatgaattt cacggatagt
      421 taatacggat caggaacgtt aagggtttct ctgctagggg ctcggttttt gaactttgaa
      481 cttattactt tgaactttgc aagctgtctc tcccacagct ccggggtgaa ccggatcgga
      541 taacgctctc atttcgttag cgaacaaagt acagtttcaa agtacaaagt tcaaagaccc
      601 aaaaccaaag accactatta gcgtcattcc caacgcaaag cctcaatggg atcgaggcgc
      661 gccgctttcc aggccggcca gagtccgaag acgataccca ccgccgccga aataccgata
      721 cccatggcca cggcccagag cggcacatat gcaggcaggg gcgaatagcg attcatcagc
      781 aggctcgcaa cttcgccgac gatcaaaccc gccaggccgc caatcgccgt caatacgaca
      841 gcttcgatca gaaattgtga aaggatgttg aattttgtgg cgcccaccgc tttacgaatc
      901 ccaatttctt ttgtccgctc agtcacagag accagcatga tgttcatgac gccaatgccg
      961 ccaatcatca gcgcgacaaa cgaaattgct gttagcacga gtgcggtggc gccggttagt
     1021 tgattgtaaa cgtcgagcag tgaatcctgc gatgagatac caaaattgtt cggcgcgttc
     1081 tgcgggacac gccggcggcg gcgcaggatg tcggcgatct catccatcgc cgactttacc
     1141 tgcgctcggg aatgggggcg cacgatgata aagaagacca cctgggggaa cggttgatcg
     1201 ggatagtact tatcgaacgt gcttatcggc atgaagatcg aattatcgcg cgactggcca
     1261 aagaaattgc cgagcggttc catcacccca atcactctat acgggttgcc gtttattctg
     1321 atttccttgt cgaccgcgtt ctcgtaaggg aaaagcgcgc gcatcacgtc gacacccagg
     1381 acgcaaacat tggaacggtc ctgcatgtca atttctccga tgaaccgccc gctcgccaca
     1441 tactggctgt gcacaaactc gtaataggga gtcactccca gaagaatcgg cgtgtcagtt
     1501 tgtttgtcct ggtagcgagc ggttgccgcg atcatgcggt ggatgggaga gacaccggca
     1561 atggaaggcg cttcacgcaa tgcttccgca tcatccatgg tcaggtcttt gcgatgaatt
     1621 tcttcagggt tgggcggtcg gccgaaactt ggtgcgaatt tagaaacgaa gattgtattt
     1681 gagccgagcg attcgatttg gtcagcgaag gcgcggttca ggccctgaat gatcgacacc
     1741 atgaagatga tggtgacaac gcctacgctt actccaagaa tcgtcaagga cgaacgaagc
     1801 ttgttcgacc gcaaggtgtc gagtgccatc agtgaggatt cttttacatc atctaaacgc
     1861 atgggattaa ttgccgaatg ccgattgcca atcgctaatc gccattactc cgctcgcagt
     1921 gcctcaatcg gatcgagttt cgcagcgcgc caggccggcc ataagccgct aatgattcct
     1981 actaccgaag aaacaccgac gcctagaacc gccgaagcca cactcaccaa cagcgggaag
     2041 ccaatcaaca tcgagattat gtaagccaaa ccaaaaccag ttgcgactcc gagtgcgcca
     2101 ccgatggcgg ttactgtaac tgcctcgatg agaaactgcg ccagaatatc tttacgccgc
     2161 gcgccgatgg ctttgcgaat gccaatttcc ttggtgcgtt ccgtgacgga gacaagcatg
     2221 atattcatca ccacgatgcc tccgacgacg agggaaattg ccgccacccc aatcgtaacg
     2281 atgtagatgt tggaagttgc ctgcccgtag agatcgagaa acacatcctg agtctctagt
     2341 gagaatcccg catcaccgtc atcgctaaac attttgccgc gtcggttcag catgattgtg
     2401 cgtacttgat cttccgcagc ttctaattgt tccgcgctag gtacctgaat aaacgccacg
     2461 atggagctgc gcgcaccaaa cgcttgttga tatgccgagt aaggaatgta aaccacgttg
     2521 tcgcgcgaga aaccaaaaat ggatcccagc ggctccagca caccgatgac cgtgtaaggg
     2581 cgtccggcaa tgcgaatctc ctgacccact gcgcgatcag cgggcgcgtc gccaaagagg
     2641 ttcttgagga gatccgctcc cgccaccgca accggtcgcc ccgcgtcgcc ttcaccttcg
     2701 gtccaaatgc gcccggcatc gatcgaaaca ccgtcgatct caaacatcgt tagtggagtt
     2761 acgccgcgga tgcgtacgtt ttccgccttt tgatccgcgt gcttcaccgt ttcggcggca
     2821 ttggccgcta tgcctacgcg ccagcaggcc gtgcaaagtc gcgcaatcgc gtcagcgtct
     2881 tcgtcggtaa catctttgcg cttattgaaa gtgacaaatt cttctcggga tttgatgatc
     2941 gccggctttt tggaaatggt gaacaccgtc gaaccctgcg acgagaaagt ttgggcgaca
     3001 gtcttatcga gtcccttgat gatcgtgaca acggcaatca ccgcggtcac gccgacaacg
     3061 attcccatca gtgttaaggc agtgcggagt ttgtttgtgc gcagactagc cgtggccacc
     3121 aggaaggctt cccagagagc ggtgaggcgg aataagcgga agcgagattt gcctgcagaa
     3181 cggcgggata acgcgaaatc acgccgggag gcgttgggca tcccgtttga gatgatctca
     3241 tcgggctgat ggcgtttcat tagagctcac gctgacgca
//
LOCUS       DASUEX010000209         4018 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1073812, whole genome shotgun sequence.
ACCESSION   DASUEX010000209 DASUEX010000000
VERSION     DASUEX010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4018
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1073812"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="VFV61_03505"
     CDS             complement(<1..247)
                     /locus_tag="VFV61_03505"
                     /inference="COORDINATES: protein motif:HMM:NF040124.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6526 family protein"
                     /protein_id="HEX4899569.1"
                     /translation="MNPQTFANHTRWHAPFHFFLAPVMIINLIWSIVVFAKNPELETG
                     RWLIVSLALVVLGALVRTYSLRVAINSSTTSSIFLNVI"
     gene            complement(372..2234)
                     /locus_tag="VFV61_03510"
     CDS             complement(372..2234)
                     /locus_tag="VFV61_03510"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4899570.1"
                     /translation="MSTAIEEKKKRVTAATAWRDARELIWTHRYRLMVGMLLMLINRL
                     VGLVLPASSKYLIDDVVLKQRSDLLIPLALAAGAATLVQAFTSFTLSQVLGVAAQRAI
                     TDMRKRVQEHVARLPIRYFDSTQTGVLISRIMNDAEGIRNLVGTGLVQLIGGLVSAAI
                     AMIVLFYLNWRLTSITLLALAAFGGVMALAFKKLRPLFRERGKIHAEVTGRLTESLGG
                     IRIVKAYAAEKREELVFARGAHRLFRNVARSMTAVSGVTAFSTIIVGVVGVILILVGG
                     QAVLAGQMTLGDLFMYMIFTGLVAAPLVEIASIGTQITEAFAGLDRIREIQQMATEDA
                     DEAQRAGLETITGEIAFRDVSFEYNDDSPVLRHVSFVAPAGSTTALVGSSGSGKSTLI
                     SLVMAFNRPLTGEVTVDGRNLASIRLKDYRNQLGVVLQDNFLFDGTIAENIGFSHPHA
                     TREEIIAASRIAHCEEFIAGFADGYDTIVGERGVKLSGGQRQRVAIARAILAKPKILI
                     LDEATSSLDSESEAMIQDGLQTLRRGRTTFVIAHRLSTIRSADQILVLEGGEIVERGT
                     HEELLAAAGRYKQLYDKQYLFERNQFINPGEDFTPELPKATPAAADSTTIPTNL"
     gene            2435..2770
                     /locus_tag="VFV61_03515"
     CDS             2435..2770
                     /locus_tag="VFV61_03515"
                     /inference="COORDINATES: protein motif:HMM:NF015899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HEX4899571.1"
                     /translation="MENVLSDGACLLLPSMPYGKLALPRATLLHSLVPNKNQVQKGLP
                     MALSQDLLDILACPACKAPVELKPDGSGLKCVSCRRVYPIRDDIPVMLIDEATIEDSS
                     EDEEPARHR"
     gene            2745..3917
                     /locus_tag="VFV61_03520"
     CDS             2745..3917
                     /locus_tag="VFV61_03520"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEX4899572.1"
                     /translation="MKSLRDIVESSPHLRATAMHSLRLEEEPQPLGPPLWDWAEVRSV
                     LVIRLRSIGDTVLATPSLHALKRFLPDARIDILVEDWVAPVLEGFPYVDNVLTLKRNS
                     TTARARAMRQLRSLRYDVVYNLHGGTTATLLTRASGATRRVGLATYQYSRLYTHLAPS
                     PLILWKRAQTHSVEQQLALLGWTGVPVSDRPPTQLAVVDHAAVSVSTRLRAAGWNEQQ
                     PLAVIHPAAAFATKQWSAENFARVAEHLYDRGLAVVVITAPAEAEVGALVEGLSAAPV
                     INFSDLSLPEVTALASRARVFVGNDSGIAHIAAAVGLPSVVIFGSSNVAHWRPWATAP
                     AEVVLEEMDCQPCHGYYCEKFAQPECILRVPLERVIGAVDRVLRESEWQSRNGDGP"
BASE COUNT          902 a         1102 c         1127 g          887 t
ORIGIN      
        1 tgattacgtt caaaaagata ctgcttgtcg tagagctgtt tatagcgacc cgcagcgaat
       61 aagttcgcac cagggctccg agcactacca gggccagcga gacgatcagc cagcgtccgg
      121 tttcaagttc cgggttcttc gcgaacacca caatcgacca gattaagttg atgatcatca
      181 ccggggctaa gaaaaaatga aacggcgcat gccaacgagt atggttagcg aacgtttggg
      241 gattcatcaa ttttgctcct gtagataggt ttgatcggag atagtgtttg aatctcgatg
      301 gtctctgacc tagcggtgcg cgtaagtctt cgactcagcg ctttctgcct actgcctact
      361 ggccggctgc cctagagatt cgtcggtatc gtggttgagt cggctgccgc cggcgtggct
      421 ttgggcaatt ccggagtgaa gtcttcgcca ggattgatga actgattacg ttcaaaaaga
      481 tactgcttgt cgtagagctg tttatagcga cccgcagcgg cgagcagttc ctcgtgagtg
      541 ccgcgctcga caatctcccc accttccagc accaggattt gatccgcact gcgaatcgtt
      601 gagagtcgat gcgcgatgac gaaggtggtg cgtccgcgcc tcagggtctg caagccatcc
      661 tgaatcatcg cttcactttc actgtcgaga ctggaagtgg cttcgtccag aatcaggatt
      721 tttggcttag ctagaatcgc acgcgcgatg gctacgcgct gtcgctggcc gcctgacagt
      781 tttacgccgc gctctcccac aatcgtgtcg taaccgtcgg cgaagccggc gatgaattct
      841 tcacagtggg cgatgcggct ggcggcgata atctcttcgc gagtcgcatg aggatgggaa
      901 aacccgatgt tctccgcaat cgtgccgtcg aacagaaaat tatcctggag caccacacca
      961 agttgattcc ggtaatcctt gagccggatt gaagccagat tgcgtccgtc cacagtcact
     1021 tcacccgtga gcggtcgatt gaaggccatc acgagactga tcagtgtcga cttaccggag
     1081 ccgctcgatc cgacgagtgc tgttgtggac ccggcgggcg cgacgaagga tacgtgacgc
     1141 agtaccggag agtcgtcgtt gtattcgaag ctgacgtcac ggaaagcaat ctcgccggtg
     1201 atcgtttcca gccccgctcg ttgcgcttca tctgcatcct ccgtagccat ttgttggatc
     1261 tcacgaatcc gatcaaggcc ggcaaaggct tcggtgatct gcgtgccgat agaagcaatt
     1321 tccacgagtg gtgcggctac cagtccggtg aagatcatgt acatgaacag atctccaagt
     1381 gtcatctggc cggcaagtac tgcctggccg cctaccagga tgagaatcac gcccacaaca
     1441 ccgacgatga tggttgagaa agcggtaact ccggagactg cggtcattga gcgagcgacg
     1501 ttgcggaaca aacgatgagc gccacgcgca aacactaatt cttcgcgctt ctcggcggcg
     1561 taagccttga cgatgcgaat gccccccagc gactcggtta accgtcctgt cacctccgca
     1621 tgaattttcc cgcgctcgcg aaagagcggc cgaagtttct taaaggctaa ggccatcacg
     1681 ccgccgaaag ccgcaagggc gagcaaagtg attgaggtca aacgccagtt caaataaaag
     1741 agcactatca tcgcaatggc ggcactgacg agaccaccaa tcagttgcac caacccggtg
     1801 cccaccaggt tcctgatgcc ttcggcgtca ttcatgatgc gggagatgag cacgccggtt
     1861 tgggttgagt cgaagtatct gattggtaaa cgagccacat gttcctgcac tcgtttgcgc
     1921 atgtcggtaa tggcccgctg cgcggccacc ccgaggactt gcgacagagt gaacgatgtg
     1981 aaggcctgca ccagcgttgc ggctccggca gccaatgcca gcgggatcag taggtcgctg
     2041 cgttgtttca gcaccacgtc gtcgatcaga tactttgagc tagcgggcag gacgagacca
     2101 accaaccgat taatgagcat tagcaacatt cccaccatca gccggtagcg atgagtccag
     2161 atcaattctc gagcatcgcg ccaggccgtg gcagcggtta cgcgtttttt cttttcttca
     2221 atggcggtgc tcattggtgg atctaaaggt aacctagcct aaaccaaaag gcgcgacaag
     2281 ggaggggccc ctttagattg ccgattggcg atttctgatt gccaatcggc ccggatgatt
     2341 gtcggtattt gccggtcttc aaaacgaacc acgaaattgc accaatcaag actaaacaac
     2401 ggccaaatga agaatgaaag atctgaaatg acatatggaa aatgttcttt ctgatggagc
     2461 ctgcctactg cttccttcca tgccctatgg taagcttgcc ctaccgagag ctactttgct
     2521 tcactccctg gtgccgaata aaaatcaggt tcagaaaggg cttcctatgg cactcagtca
     2581 agacctgtta gacatactcg cctgccccgc ttgtaaagca ccggtagaat tgaaaccgga
     2641 tggcagcgga ttgaaatgcg tctcctgtag gcgcgtctat ccaattcgtg atgacatccc
     2701 cgtcatgttg atcgacgaag caacgattga agattccagc gaagatgaag agcctgcgcg
     2761 acatcgttga atcttcccct cacttgcgcg cgacggctat gcacagtttg cgccttgaag
     2821 aagaaccgca gccgctcggt ccaccacttt gggactgggc ggaggtgcgc agcgtacttg
     2881 tcatcaggct gcgttcgatc ggtgatacgg tcctggccac tccttcgctt catgcattaa
     2941 agcggtttct ccccgacgct cgcatcgaca tacttgtgga agactgggtg gcgcccgtgt
     3001 tggaaggctt tccttacgtc gacaacgtgc tgacgcttaa acgaaacagc acgacagcgc
     3061 gcgcccgcgc tatgcgccag ctgcgatcct tgcgttatga cgtagtctat aaccttcacg
     3121 gcggcaccac ggccacatta ctgacgcgcg cctccggcgc aacgcggaga gtcgggctgg
     3181 caacgtatca gtacagccgc ctctacaccc atctggcgcc ttcgccgttg attctatgga
     3241 aacgtgctca gacgcattca gttgaacagc aactagcgtt gctgggttgg actggagttc
     3301 ccgtaagcga tcggccgcca acgcaacttg ccgtggttga ccatgctgct gttagtgtct
     3361 ccacgagact gcgagcggca ggttggaatg aacaacaacc actggccgta attcatccgg
     3421 cggcggcatt tgctacgaag caatggtcgg cagagaactt cgcgcgcgtc gcggaacacc
     3481 tgtacgatcg cggactcgca gtggttgtca tcaccgcacc tgccgaagcc gaagttggag
     3541 cgttggtcga aggtctttca gcggcgcccg tgatcaattt cagcgatctc tctttgcctg
     3601 aggtgacggc gttagcatca cgagcgcgag tatttgttgg taacgacagc ggcattgccc
     3661 acatcgccgc tgcggtgggt ttgccgtccg tagtgatctt cggatcgtca aacgtcgcgc
     3721 attggcgccc ctgggcaaca gcgccggctg aagtcgttct ggaagagatg gactgccagc
     3781 cgtgtcatgg ctattactgc gaaaagtttg cgcagcctga atgtattctg cgcgtgccgt
     3841 tggagcgggt aattggcgcg gtcgatcgag ttttgagaga aagcgagtgg caaagccgta
     3901 acggagatgg cccatgactt tcgaggcaaa gccggtagag atcaaacgga gctaagtgtg
     3961 caccagctaa ctgctcaacg ggcaaaagaa atcattacct ccatgcgggg ccgcaagg
//
LOCUS       DASUEX010000210         2038 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1167022, whole genome shotgun sequence.
ACCESSION   DASUEX010000210 DASUEX010000000
VERSION     DASUEX010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1167022"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..1039)
                     /locus_tag="VFV61_03525"
     CDS             complement(116..1039)
                     /locus_tag="VFV61_03525"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX4899573.1"
                     /translation="MLFKSALIFLVLAVFAGCAATPPAPPVVEPVGEEITSPEPPPPP
                     KIALVLGGGAAKGFAHIGVIKALESHAITPDIIIGTSAGSVVGALYAGGYNGFDLQRV
                     ALSMDEGTVSDWVLPNRGFIKGESLQKFVNNAVQNRPIEALNRTLAVVATDLQSGELV
                     VFERGDTGMAVRASSSVPGIFRPVMINGREYVDGGLVSPVPVKTARDLGADIVIAVDI
                     SDRPKTARLRDTIDVLMQTFIIMGRTIASQELTRADIVVVPDITNLNSTNFDSRNYAI
                     IEGEKAGLAAVPKLQEKVAAYYESKKTAVPR"
     gene            complement(1118..1591)
                     /gene="bfr"
                     /locus_tag="VFV61_03530"
     CDS             complement(1118..1591)
                     /gene="bfr"
                     /locus_tag="VFV61_03530"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HEX4899574.1"
                     /translation="MKGDKQVLKHLNQVLANELTAINQYFLHARMFKNWGLKKLDEHE
                     FKESVDEMKHADRLIERILFLEGLPNLQDLGKLLIGENVQECLKCDLQLEMDAHPALR
                     EAIAYCESSGDYVSRELFEEILESEEEHIDWLETQLNLIQKIGIQNYMQSQISGS"
     gene            1739..1918
                     /locus_tag="VFV61_03535"
     CDS             1739..1918
                     /locus_tag="VFV61_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899575.1"
                     /translation="MEDLLGAVGAPAAATGHTQTQMEFDQRTGAPAHCVADLAFGDAV
                     AKANVHGWAMDEARM"
BASE COUNT          418 a          595 c          594 g          431 t
ORIGIN      
        1 aacccgccgg ttcgatagcc cgatgtattg ggaacatcgg gttggacgct gatacgcagt
       61 aagtgcagcg acggtcccag gccgcatgca atggtaggtg cggtaacact ttctactacc
      121 ggggcacagc cgttttcttg ctctcgtaat acgccgcgac tttttcctga agtttcggta
      181 cggccgcaag cccggccttt tcgccctcga tgatggcgta gttgcgcgag tcgaagttgg
      241 tcgagttcag gttggtgatg tccggaacca caacgatatc ggcgcgcgtg agttcctggc
      301 tggcgatggt ccgccccatg ataatgaagg tctgcatcaa gacgtcgatc gtatcgcgca
      361 gcctggcagt cttcggccgg tcggaaatgt ccacggcgat cacgatgtcg gctccgaggt
      421 cgcgcgcggt ctttaccgga acagggctta cgaggccgcc gtccacatat tcccggccat
      481 tgatcatcac cggccggaaa atgccgggta cgctgctcga agcgcgcacc gccatgccgg
      541 tgtcgccgcg ctcgaaaacg accagttcgc cggactgcag atcggttgcc acgaccgcca
      601 gagtgcggtt gagcgcttct atgggccggt tctggaccgc gttgttgacg aatttctgca
      661 gcgattcacc tttgatgaat ccgcggttcg gcaagaccca gtcgctgacg gtgccctcgt
      721 ccattgacaa cgccacgcgc tgcaggtcga acccgttgta accaccggca taaagcgccc
      781 ccaccacgct gccggcactg gtaccgatga tgatgtcagg cgtgatcgca tgcgactcga
      841 gcgccttgat caccccgatg tgggcaaagc ccttggcagc cccgccgccg agcacgagcg
      901 cgatctttgg cggcggtggc ggctccggcg aagtgatttc ctctccgacg ggctcgacga
      961 cagggggcgc tggaggcgtg gccgcgcaac ctgcgaacac cgcaagtacc aaaaatatca
     1021 acgcactttt gaacaacatc ggttcggatt tcttccgcct aacagcgagt ctggagactg
     1081 tgtagcggtt agcatgccct cgcccacgaa ttcagtctca actcccagaa atctgcgact
     1141 gcatgtagtt ctgaattccg atcttctgga tcagattgag ttgggtctcg agccagtcga
     1201 tatgctcttc ctcactctcc aggatctctt cgaagagctc gcgggaaaca tagtcaccgg
     1261 aactctcgca atacgcgatc gcctcgcgca gcgccgggtg cgcgtccatc tcgagctgca
     1321 ggtcgcattt cagacactcc tgcacgttct cgccgatcag cagcttgccg agatcctgga
     1381 ggttcggaag tccctctaga aacaggatgc gctcgatcaa cctgtcggcg tgcttcatct
     1441 cgtccaccga ctccttgaat tcgtgctcgt cgagcttctt caggccccag ttcttgaaca
     1501 tgcgggcatg aagaaaatac tgattgatcg cggtcagttc gttggcgaga acctggttga
     1561 gatgtttcag gacctgcttg tcgcccttca tgtgcgcccc ctggtctgct tgtgacgaat
     1621 caagcacgca ttataggtcg cgctacgcgc agccagaaga aagaacaggg atcagaaacg
     1681 agaggaacta cgcgacgttc aactgctcgc gcggcgattg gcccgatgaa agcgtttcgt
     1741 ggaggacctg ttgggcgcag tcggcgcacc tgccgcagca actggccaca cccagacaca
     1801 aatggagttc gatcaacgaa cgggcgcccc cgcccactgc gtcgcggatc tggctttcgg
     1861 tgatgccgtt gcaaaggcaa acgtacatgg ttgggctatg gatgaggcgc gcatgtgata
     1921 ctaaatcaca atgagaatga ttgtcaataa cggaatggga tgtaggatgc ggaaaatgaa
     1981 cacatccgcc agggggcgat tggcgtcgtc gtcaaatacg tgtactgatc ttcacggg
//
LOCUS       DASUEX010000211         2691 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_615684, whole genome shotgun sequence.
ACCESSION   DASUEX010000211 DASUEX010000000
VERSION     DASUEX010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2691
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_615684"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(161..598)
                     /locus_tag="VFV61_03540"
     CDS             complement(161..598)
                     /locus_tag="VFV61_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899576.1"
                     /translation="MKNQILRSVAIFGLFFMLAMTSVQAQTPSRVEVKVPFDFAAGKV
                     LFKAGIYSIKKVSDNTLAIRSIDGKTTKLVDAPLTIGSRDFKAGERLVFNQYGNQYFL
                     SQVWLSSDSGRQLFSGAETKAAREYRLAHNNAKPQRVEIAARR"
     gene            1043..1612
                     /locus_tag="VFV61_03545"
     CDS             1043..1612
                     /locus_tag="VFV61_03545"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX4899577.1"
                     /translation="MPQTSQNVTQLLIGWSNGDKEALDALLPLVYEELRKQAAGYLRR
                     ERPGHTLQTTALIHEAYLKLVDQKNVHWQNRAHFFGIAAQLMRRILVDHARSKKRVKR
                     GGSDIRVSFNEANVLGQTKDLDIVALDEALARLAEIDEQQSRIVELRFFSGLTVEETA
                     EVLAISPATVKRDWSMAKAWLHREISGEA"
     gene            1616..>2691
                     /locus_tag="VFV61_03550"
     CDS             1616..>2691
                     /locus_tag="VFV61_03550"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX4899578.1"
                     /translation="MSDVSKKHWQQVKDVFEGALERQGAEREAFLDSACSSDLALRKE
                     VESLLRSYGQAGSFMEAPAVALAAESLIGEQKKLLVGQKVKHYQVVASIGEGGMGEVY
                     LAKDTILGRRVALKVLPEYVGKDPDRLRRFKQEARSASTLNHPNVCVIHEIGETEDGR
                     PFIAMEHIEGLTLRQRMNDHALKLGEALDIAIQVAAALTAAHEARIVHRDIKPENIMI
                     RRDGYVKVFDFGLAKLTEQRQHADNATMSTLLVNSSPGTVMGTAAYMSPEQARGVPVD
                     ERTDIWSLGVVLYEMVSGRPPFSGQTPTDVVVAVVEKDPPALSQYFAGAPAEFERIIR
                     KALRKDTDQRYQIVKEMAIDLRSLR"
BASE COUNT          651 a          692 c          763 g          585 t
ORIGIN      
        1 caagcccggt atgaatgaaa gtgaactagt ctctcaaggt attagttcca tctaagcagc
       61 gagcgatgga tcctgatcca aaatagagac ttgaagtgcg aaagggcgga caaggtgtcc
      121 gccctcaaca agtcgagtgc ctgagttgta agcgactcta ttatctgcga gccgcgatct
      181 ctacacgttg cggcttcgca ttgttatgcg caagtctata ctcgcgtgcg gcctttgttt
      241 ccgctccaga aaacaactgc ctgccgctgt cgctgctcaa ccagacttgg gataggaagt
      301 attggtttcc atactgattg aagaccaggc gttcgcccgc cttgaagtct cgtgagccga
      361 tggtaagtgg cgcatcgacg agcttcgtgg ttttcccgtc aatgctgcga attgccagcg
      421 tattgtcgga cacctttttg atactgtaga ttcccgcctt gaacagcact ttccccgcgg
      481 cgaaatcaaa cggaacctta acttcgaccc tgctaggtgt ttgcgcctgc acgctcgtca
      541 tggccagcat gaagaagagg ccgaatatcg cgacacttcg tagaatttgg tttttcatat
      601 gacctcctta cggtcctaat ttcaggccag ccctattgcc gccttcacct gttaacgcga
      661 caaccggaag caaaggggct cacggggcta aaaataattt gctgcctgaa ataaactgcc
      721 aagacgccaa ggcacgccaa gatcgccaag gctgcaaaca accggattaa gtacgagctc
      781 aaatccaagt ccagaactca attccagacc ctgaactcat caaggctttg attgagctca
      841 aaccgtattg aagactcagc ggacttggcg gctccaggca tcctgccgtt atttgttgcc
      901 cacctgagta atgagcccca aactccccga agctcgcgtt attaaataag gagcagtgtg
      961 gccgcagcgt ttgcccccac gaacggaatt cggtgcaccg tcgtacaatg ggggcaatcg
     1021 ttaattcggt gggtagtggt gcatgccgca aacgtcacaa aacgtaactc aactgctgat
     1081 cggctggagc aacggtgaca aggaagctct cgacgcgttg cttccgctgg tctatgagga
     1141 gctgcgtaag caggcggccg gatatctgcg acgcgagcgg cccggacata cgctgcaaac
     1201 gacggcgctg atccacgagg catatttgaa gctggtggat caaaagaacg tacactggca
     1261 aaaccgcgcc cacttctttg gtatcgcagc tcagttgatg cggcgcattc tcgtggatca
     1321 cgcgcgtagc aaaaagcgcg tcaagcgcgg cgggtcggac attcgcgtat cgttcaatga
     1381 ggcgaatgtg ctcggacaaa cgaaggacct cgacatagtt gccctcgatg aggcactggc
     1441 gcgtttggct gaaatcgacg agcagcaaag caggattgta gagttaagat tcttcagcgg
     1501 acttacagtt gaagaaactg ccgaagttct ggcgatttct cccgcgacag tgaagcgcga
     1561 ctggagcatg gccaaggcgt ggttgcaccg agagattagc ggtgaggctt gaaggatgag
     1621 cgacgttagc aagaaacact ggcagcaggt gaaggacgtc tttgaaggcg cgcttgaacg
     1681 gcagggcgcc gaacgcgaag cgtttcttga tagtgcctgt agcagtgatc tcgctctgcg
     1741 taaggaagtg gagtcattac tgcgttcgta tggacaggca ggcagtttta tggaagcgcc
     1801 ggcggtggcc ttagctgccg agtcattaat cggtgaacag aaaaaactgc tggtggggca
     1861 gaaggtaaaa cactatcagg tcgttgcctc gatcggtgaa ggtgggatgg gcgaagtcta
     1921 tctggcaaaa gacacaatcc tgggccggcg tgtggcgttg aaagttctgc cggaatacgt
     1981 cggcaaggat ccggatcgct tgcgacgttt taagcaggaa gcgcgcagcg cctcaacact
     2041 aaaccacccg aatgtttgtg tgattcacga aatcggcgaa acagaagacg gccggccctt
     2101 catcgcaatg gaacacattg aggggctgac gctgcgtcag cggatgaacg atcacgcttt
     2161 gaagctcggc gaagcgttgg atatcgcgat tcaggttgcc gccgcgctca cagcggcgca
     2221 tgaagcgcgc atcgtccatc gcgatatcaa gccggaaaac atcatgattc gtcgcgacgg
     2281 ctacgttaag gtctttgatt ttggactcgc caagctaacc gaacaacggc agcacgcgga
     2341 caacgcaacg atgtccactt tgcttgtgaa ctcctcaccc ggcaccgtga tgggtacggc
     2401 ggcttatatg tcgccggagc aggctcgagg tgttcccgtc gatgaacgaa ccgacatctg
     2461 gagtttagga gttgtgcttt atgaaatggt ctcggggcgt cctccgtttt cgggtcagac
     2521 tccgaccgat gtcgttgtcg ctgttgttga aaaggatccg cccgcacttt cccaatattt
     2581 tgccggcgcc ccggctgagt tcgaacgtat catcagaaag gcactgcgca aagacacgga
     2641 ccagcgttac cagatagtca aagagatggc tatcgattta cgaagcctgc g
//
LOCUS       DASUEX010000212        10907 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1533046, whole genome shotgun sequence.
ACCESSION   DASUEX010000212 DASUEX010000000
VERSION     DASUEX010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 10907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10907
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1533046"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..909)
                     /gene="amrB"
                     /locus_tag="VFV61_03555"
     CDS             complement(<1..909)
                     /gene="amrB"
                     /locus_tag="VFV61_03555"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HEX4899579.1"
                     /translation="MGNESSLVFSGIVPHPPIMVPEVGREAIGEVRQSISAMGELTRR
                     VIANKAATVILISPHAPLEPYAFVAYQDHELHADFAAFRAPKVQVTAFLDQELLNAIA
                     KRGAEENYTVVGISDAKLDHGSAVPLYFLQRNGWRGKVVALGYSFLSNEDHLRFGSCI
                     GRAIRDVDRPVALIASGDLSHRLKPDAPAGFNPTAYIFDDEIVDALRSSDPERIVQIN
                     QNLRELAGECGYRSMLIAVGATRELPLSCEVLNYEAPFGVGYMVAQLTSKERSKERIA
                     TTGLAADSSEADGENLPALARAAVERF"
     gene            1169..2392
                     /locus_tag="VFV61_03560"
     CDS             1169..2392
                     /locus_tag="VFV61_03560"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX4899580.1"
                     /translation="MMRQMKQFDVLIVGAGPAGSFAAELLARGGKRVALFDGRPEGEP
                     KACGGGVTAKALKAWPQLLNAVGRTVDELDLYSPSGKRLHLQLDEPFAIYSRIAFDSY
                     LRERARSVGAEVIAAKISGRAFRKHEDGWQVKNLVGDEWTGRMLVGADGAGSPIARML
                     AGPLPPSEMEVAFGYRAPLPKNAEAPTVVAFLPGWVGYAWAFPRLDHISFGIATTQDA
                     FIHKPLDDLLWDFMIGYYRQREDATAKLWSARRIDQERDKLLLDKLKNTAERYAARIP
                     GLNATTWDTRKVSGEDWALIGDAAGFADPVTGEGIYYALRSAELLAECHLVGNPDQYE
                     QRWREDFGAELKRAAQMRPRFYGNFWGAPFTERMIEFARGHRGIKRVLGDLVAGEQGY
                     TDLKKKLAKSAIRPL"
     gene            complement(2527..3159)
                     /gene="pdxH"
                     /locus_tag="VFV61_03565"
     CDS             complement(2527..3159)
                     /gene="pdxH"
                     /locus_tag="VFV61_03565"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF004231.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEX4899581.1"
                     /translation="MENISSANKAAELDERNAETNPIRQFQIWFDDAVGRSLPLAEAM
                     TLCTATADGKPSARMVLLKSVDESGFVFYTNYRSPKARELDANPFAAMVFYWEPLERQ
                     VRVEGKVARISADESDEYFQTRPRESQLGALASPQSAVIDSREVLEQRFRELEDEYRD
                     RTIARPDHWGGYRLVPDRIEFWKGRPGRLHDRLLYEAQTDGSWKRSRLAP"
     gene            complement(3167..3589)
                     /locus_tag="VFV61_03570"
     CDS             complement(3167..3589)
                     /locus_tag="VFV61_03570"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX4899582.1"
                     /translation="MTKYTIEERVRWGDVDAASIIFYGAYIRFFEFAETELFRAVGLP
                     YGVMFEELDIWLPRVHLECDFRHAAQLDDLLEVSVYVGRFGNKSMRLNFEVRRKGEDL
                     LIAEAHFVLAAVKRDSFETVPIPEMLKARLAPFTQAAG"
     gene            complement(3785..4612)
                     /locus_tag="VFV61_03575"
     CDS             complement(3785..4612)
                     /locus_tag="VFV61_03575"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX4899583.1"
                     /translation="MTSFKVPQRTSVGRGVDRVDIAYDDAGSGPVVVLLHGYPFNRTL
                     WRDQVMALQENYRVIVPDLRGHGASAIMPGPATMEQMAGDIAGLLDNLNIERATIGGL
                     SMGGYVALAFYRLFPFRVLSLILAATRAQADNDEAKQNREVQAAKARNEGMEGIADAL
                     LPKLLTAETVAKRPEIVKHLRGMMASTNPEGAAAALQGMATRQDQTSFLSEIAAPTLI
                     LVGSEDAITPPVDAELMHREIKGSRLEIIEGAGHVVNLEKREEFNAAIGRFLSGVAG"
     gene            complement(4609..7845)
                     /locus_tag="VFV61_03580"
     CDS             complement(4609..7845)
                     /locus_tag="VFV61_03580"
                     /inference="COORDINATES: protein motif:HMM:NF015859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE domain-containing protein"
                     /protein_id="HEX4899584.1"
                     /translation="MPVGESKLNGIRPRRYFLPYFVLLLGLLITAFSTFYVWRTAKSQ
                     DLIRFGSTTQELTTYLRGRPRIYIEVLRAGTGLFAISPTTSASQFHNFVERLELKHQY
                     PGAQGIGYIARVKRQQKASFISETRSRHHSDFTIWPEQERDEYNPVVYFEPLDNHPHQ
                     LVGFDMGSEPVRRAAMEAARDTGLPAATAHLPLVKDGEEPGDVGFLIYAPIYENDRTP
                     TTDEERRDALAGFVFSRFRAADFLKAILAIKNTTDVDISLHDGQVPTRENLIHDTATV
                     AAPINNSAYRPFRAQINIEIAGRTWTSVFTSRPEFAAAKGNSQIYVVVVGALISLLLF
                     GLTHSQVKARAATERTASELQISERKVRKTLSDRERAEEALRESEERYRELIENANDI
                     VFTLDLDGNVTSMNSAVERLTGFSREELRKMNMRQFLTAGSDAAAREMTGRKLAGEER
                     TNYEIDVQAKHGGLVRLEVSSRLIFKSGKPVGIQGVARDITARRAAEEALREADQRAL
                     SEYERLLERVSILSQALGTARELLQIFRALREFAVVSAPCDGIFVSLYDPIRDVRTAC
                     YGWGDHQEIDTSELPPMPVTATGPNSIAVRTGEVVVTNDYMNATRNHPVVLVGPDNGL
                     RPKSSLAAPMSVMGRIVGTIEIQSYEASAYRDEHVTAMRMAANLVAVAIENVRLLNQE
                     SSARATAEESNRLKDEFLATVSHELRTPLTAILGWSRMLQSDSLDQSMAANAIETIRR
                     NAKAQSQIIDDILDVARIITGKLAMELDPLELAPIIEAAVDVVRPTAEAKGVRVESEL
                     PAQPMVVAGDSNRLQQIVWNLLSNAIKFTPSGGRVVLTVRNLGAQVEINVSDNGQGIS
                     KDFLPFVFDRFRQADSTTTRQHGGLGLGLAIARHLVEIHGGAIKATSAGADQGSTFTV
                     LLPRLGSNSKLNDPKGDYATSAKALTSNKLKGIHVLLVDDDEDTLRLMTAALNQGSAR
                     VTAVSSAADALDALKSETPDVLISDIAMPNEDGYQLLAKVRALGSAQSRFLPAVAITA
                     YAREEDRLQAIASGFQAYLAKPIELSELIAVVAEAVRGVESNTEVDLA"
     gene            complement(7865..9190)
                     /locus_tag="VFV61_03585"
     CDS             complement(7865..9190)
                     /locus_tag="VFV61_03585"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX4899585.1"
                     /translation="MRFIKYSKFKGLDVSAINLGDLMEELSDSLLSSGYNDDYYWTRQ
                     RNDPDTSLDALRRALLQALMNQGLLNEYQVQEMLADNDGEFKGSALEEMLNQLIERLV
                     EEGYLKLQELPAERSQQPGAGGPQVSEPLPRNIKFEVTEKGLDFLGYKTLRNLLGSLG
                     KSSAGRHDTPQLSTGVEAAAASKRYEFGDTINLDVNTTLLSAIQREGLRVPLNLEYSD
                     LHVHQCDYQSSCATVVMLDCSHSMILYGEDRFTPAKKVALALAHLIRTQYPGDSLKLV
                     LFHDSAEELAFAELATTQVGPYHTNTAEGLRLARRILQSQRKEMKQIVMITDGKPTAC
                     FVDSNQMLAGAGVGRGRRPDQTVTRRIYKNSMGGDPFVMAETFKEVQACRKAGIMINT
                     FMLASDYYLVEFVKQMTAMTNGKAYFANPDNLSQFVLMDFLRRRTSRIK"
     gene            complement(9355..10824)
                     /locus_tag="VFV61_03590"
     CDS             complement(9355..10824)
                     /locus_tag="VFV61_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899586.1"
                     /translation="MRQPATLGELKASGYRSVSVKDELRANLIRKLKAGEKLFLGIVG
                     YDETVIPQLVNAILARHNIILLGLRGQAKSRIIRQLTELLDERIPIIAGSEINDDPFK
                     PISAYGREQLETHGDSLPIAWIERDSRFVEKLATPDVTIADIIGDVDPIKAAKGGHLL
                     SDELTIHYGLLPRANRGIFAINELPDLAGKIQVGLFNIMQEGDVQIKGYPVRLPLDVL
                     LVFSANPEDYTARGKIITPLKDRIGAEITTHYPAELPTAIQITRQEAWVDRDGWQAEL
                     HIPEFIREVVEQIAFEARDDQRVDKQSGVSQRLPITVTESVISNAERRALLTGEKVIV
                     PRISDIYAAIPSMTGKMELEYEGEQIGARRIARDLIKRAAGEIFNGYFVGIEFASTVQ
                     WFDAGNNLRLADTAAADECRRLLEAVPELIETSLIPFDFNKADTARVVAACELVLGGL
                     YAENKISRNEEGGYTAVTKAKKDRRGMIYDDLTETGKYS"
BASE COUNT         2390 a         3074 c         2950 g         2493 t
ORIGIN      
        1 aaaacgttca accgcagcac gcgccaatgc cggtaaattc tccccgtccg cctcggaact
       61 gtcagcggcg aggccggtgg ttgcaattct ttccttactc ctctccttgc ttgtgagttg
      121 tgcaaccata tagccgaccc caaacggggc ttcgtaattc aggacctcac aggagagcgg
      181 cagttccctc gtcgccccca ccgcaattaa cattgaacga tagccacact cgccggctag
      241 ctcccgcaag ttttgattga tctgaacgat gcgttcagga tcactggagc gcagcgcgtc
      301 gacgatctcg tcgtcaaaga tatacgcagt ggggttgaag ccagccggag catctggctt
      361 gagccgatga ctgaggtcgc cgcttgcgat caaagcgacg ggccggtcaa cgtcgcgaat
      421 tgcccgtccg atgcatgagc caaaccttaa gtgatcctcg ttagaaagaa agctgtatcc
      481 gagagccaca accttgcccc gccagccgtt gcgctgcagg aagtagagcg gtacggcact
      541 tccgtgatcg agtttcgcat cggaaatacc gacgaccgta tagttttctt cagcaccgcg
      601 cttggcaatc gcgttcagca actcttgatc gaggaaagct gttacttgaa ctttaggggc
      661 ccggaaagcc gcgaagtctg cgtgaagttc gtgatcctgg taagcaacga aagcgtaggg
      721 ctcgagcggc gcgtgaggcg agatcaagat tacagtcgcc gccttgttgg caatgacgcg
      781 cctggtcaat tcgcccatgg cggaaatcga ctgccgcact tcgccgatgg cttcacgacc
      841 gacctccggc accatgattg gtggatgcgg aacgatcccg gaaaagacaa gagagctttc
      901 gtttcccaat ggtggtcgcc tcccgtagga acctgagctc gggcgttgaa acgctgagca
      961 acgattggct tgtgcaggaa acggctggat tttatcacgc agcaccggga tttccgattt
     1021 ccgattggcg attgccaatt gccgattcga gacgagtatc aatgccgacc cgttctgcca
     1081 gctctaaacc gccgtcaact catcagccaa gactaatcgc gaatcggcaa ttgccaatcg
     1141 ccaattggca atcgaggttg cggctcttat gatgaggcag atgaaacagt ttgatgtctt
     1201 aattgtaggc gcgggtcccg ccggatcgtt tgctgctgaa ctcttagcgc gcggcggcaa
     1261 gagggtggca ctgttcgacg gacgtcctga gggcgagccg aaagcttgtg gcggcggcgt
     1321 cactgcgaaa gcactgaagg cctggccgca actgctaaac gccgtgggcc ggacggttga
     1381 tgagctcgat ctctattctc cctccggcaa gcgtctgcat cttcagctcg acgagccatt
     1441 cgcgatctat tcacgcatcg ctttcgattc ttatttacgt gaacgagcgc ggtcagtcgg
     1501 cgccgaagtg attgcggcga aaatttccgg acgcgctttc cgaaagcatg aagacgggtg
     1561 gcaggtaaag aatctggttg gtgatgaatg gacggggcgg atgttagtgg gcgcagatgg
     1621 agctggaagc ccgatcgcac gcatgttggc cggtccgttg ccgccttcgg agatggaggt
     1681 agcttttggt taccgtgctc ccctccctaa aaacgcggaa gcgccgactg tcgttgcctt
     1741 cctgccgggc tgggttggct acgcctgggc atttccgagg ctggatcata tttcgtttgg
     1801 catcgccact acgcaggacg cattcataca caagccgctt gacgatttgt tgtgggattt
     1861 catgattggt tattaccggc aacgcgaaga tgcgacagca aaactgtggt ctgctcgcag
     1921 gatcgatcag gaaagagaca aacttcttct ggacaaactg aaaaataccg ccgagcgcta
     1981 tgcggcacgc attcccggac tgaacgccac aacgtgggat acgagaaagg tgtccggcga
     2041 agactgggcg ctgataggcg atgcagcagg cttcgcagat ccggtaaccg gcgaaggtat
     2101 ctactatgcg ctacgctccg cggaattgct tgcggagtgc cacctggttg gcaacccaga
     2161 tcaatatgaa cagcgctggc gcgaagactt tggagccgag cttaaacggg cagcacagat
     2221 gcgcccgcgc ttctacggaa acttctgggg cgcaccgttt acggaacgaa tgatcgagtt
     2281 tgcgcgcggc catcgcggca tcaagcgcgt gttgggtgat ctggttgccg gtgagcaggg
     2341 atatacggat ttgaaaaaga agctggcgaa gagtgcgata cggccgttgt agcaacctaa
     2401 tgtgatgaag tgaactgagg cttcccaaaa caatgctcag tccagcagtg tggattacct
     2461 ccctggttgg aattgattca accctggcag cggcaagccg gtcttcctaa ctgaggatct
     2521 caaactttat ggcgccagac gactacgttt ccaactgcca tcggtttgcg cctcgtaaag
     2581 cagccgatca tgtaagcggc ctggtctgcc tttccaaaac tcaattcgat caggtaccag
     2641 ccgatagccg ccccagtgat caggtcgggc aatcgtccgg tcgcggtatt catcctcaag
     2701 ctctcggaaa cgttgctcga gtacttcgcg actgtcaatg acagcgctct gtggcgaagc
     2761 aagcgcgccg agttggctct cacgcggccg ggtttgaaaa tactcgtccg actcgtccgc
     2821 agagatccgc gcgacttttc cttcaactct cacctgccgt tcgagtggct cccaatagaa
     2881 caccatcgcg gcgaaaggat tcgcgtccaa ctctcgcgct ttgggactgc ggtagttggt
     2941 gtagaaaacg aagccagatt cgtcgacgct cttgagcagc accattcgcg ctgagggctt
     3001 gccgtccgca gttgccgtgc acaacgtcat cgcctcggca agcggcaggc tgcgaccgac
     3061 agcatcgtca aaccaaattt gaaattgacg gatcggattc gtctccgcat tgcgctcatc
     3121 cagttccgcg gctttgtttg cgcttgagat gttttccacg gcgttactat ccagcagctt
     3181 gagtaaaggg agcgagcctg gctttcagca tttccggaat cggaactgtt tcgaagctgt
     3241 cgcgtttcac ggcggcgaga acgaaatgcg cctcggcgat caacaagtct tcacccttgc
     3301 ggcgcacttc aaaattcagc cgcatggact tgttcccaaa ccgtccgaca taaaccgaaa
     3361 cttccaacaa gtcgtcgagc tgagcggcat gacgaaaatc acattcgaga tgaacccgag
     3421 gcagccagat gtcgagttct tcgaacatca cgccgtaagg taagccgaca gcgcgaaaca
     3481 gctccgtttc tgcgaattca aagaaacgaa tataggcgcc gtaaaaaatt atactggcgg
     3541 catcaacatc accccaacgt accctctctt cgatcgtgta tttagtcatg ggtgacggat
     3601 ccgggtctca ggtgtcgggt ctcaggtgtc gggtctcagg tctcaggtgt cgggtctcaa
     3661 gtctcaagtc ctcaagtctc agagtcgtcg tcccgcacca accagaagac cctaccgggc
     3721 cctcgaaacc tgaaatctca gacctgagac ctgagacctg agacctgaga cctgagactt
     3781 ttatttaccc ggcgacaccg ctgagaaacc ttccgatcgc cgcgttaaat tcttcccgtt
     3841 tttccaggtt cacgacgtga ccggcgcctt caatgatttc cagccgcgag cccttgattt
     3901 cgcgatgcat tagttctgca tctacaggcg gagtgattgc atcttcactg ccaaccagaa
     3961 tcaaagtcgg cgccgcgatc tctgacaaga atgaagtttg atcctgacgc gttgccattc
     4021 cctgcagtgc cgcggcggct ccctcgggat tcgtgcttgc catcattccg cgcaggtgct
     4081 taacaatctc aggacgcttc gctactgtct cggccgtcag cagcttgggc aacagggcat
     4141 cagcaattcc ttccatccct tcgtttcgcg ccttagccgc ctgcacttcg cgattctgtt
     4201 tcgcctcatc gttatcggcc tgcgcgcgcg tggcggccag aatcagggaa agcactcgaa
     4261 aaggaaacag gcgatagaag gccaacgcga cataacctcc catcgaaagc ccgccgatcg
     4321 tggctcgttc gatgttcagg ttgtccaaca atccggcgat atccccagcc atctgctcca
     4381 tcgtcgccgg gcccggcatg atcgcacttg cgccgtgtcc ccgcagatcg ggaacgatga
     4441 cgcgatagtt ctcctggagc gccatcacct gatcacgcca caatgtccga ttgaaaggat
     4501 agccatggag gaggacgacg actgggcccg agccagcgtc gtcatacgcg atatcaacgc
     4561 gatcgactcc ccgccccacg ctcgttcgct gaggaacctt gaaactcgtc acgctagatc
     4621 cacctctgtg ttcgattcaa ctcctcgaac tgcttccgct acgacggcaa tcaattccga
     4681 caactcaatc ggcttcgcca gatatgcttg aaagccggac gcgatagctt gcaaacgatc
     4741 ctcctcgcga gcataagcag ttattgctac agccggcagg aatcttgatt gagcggagcc
     4801 caaagcgcgc acctttgcca gaagttggta accgtcttca ttgggcatag cgatatcgga
     4861 aatcaagaca tcgggtgtct cagacttcaa tgcgtctaga gcatccgctg ctgaggaaac
     4921 tgccgtgacc cgagcgctgc cctggttcag ggccgctgtc atcaaccgta aggtgtcttc
     4981 gtcgtcgtca accaacagca cgtggattcc cttaagtttg ttcgacgtca aggcctttgc
     5041 tgaagtcgcg tagtctcctt tgggatcatt caattttgag ttggaaccga gtcgtggcag
     5101 caatacggtg aaagttgatc cctggtccgc gccagcgctg gtcgccttga ttgctccgcc
     5161 atggatttca acgagatggc gcgcgatcgc caatcccaat cccaggcctc catgttgccg
     5221 cgtcgtggtg ctgtctgcct gtcgaaaacg atcgaagacg aaaggaagga agtctttact
     5281 aatgccctgg ccattatcgc tcacattaat ctcaacctgc gcaccaagat tgcggactgt
     5341 caacacgacc cgtccaccgc tcggtgtgaa cttgatggcg ttggaaagaa ggttccaaac
     5401 aatttgttgc aagcggttag aatcgccggc gacgaccatt ggctgggccg gcagttcgct
     5461 ttcgacccgt acgcctttag cttcagccgt cggtcgcacg acgtctacgg cggcctcgat
     5521 tattggagcc agctcaagcg gatcgagttc cattgcgagc ttgccggtaa tgatccgtgc
     5581 cacatcgagg atgtcatcga tgatttgcga ctgcgctttg gcgttgcgcc gaatcgtttc
     5641 aatcgcattt gccgccatcg attgatcaag tgaatcagac tgtagcatcc gcgaccagcc
     5701 aaggatggct gtcaaaggtg tacgcagttc gtgcgaaacg gtagcgagga attcgtcttt
     5761 caaacgatta gattcttcgg cggtcgcgcg ggcactgctt tcctgattca acaatctgac
     5821 gttttcaatg gcaacagcta cgagatttgc tgccatgcgc atcgccgtca catgttcgtc
     5881 gcgataggca ctagcttcat aactctgaat ttcgatagtt ccgacaatcc ttcccatcac
     5941 tgacatgggc gctgccagcg acgacttcgg tcgcaaccca ttgtccggtc cgaccagcac
     6001 cacggggtgg ttgcgggtgg cattcatgta gtcgttggtg actaccactt cgccggtgcg
     6061 gaccgcaatg ctgttgggtc cagtagcggt gaccggcatg ggcggcagtt cgctggtatc
     6121 tatttcctga tgatcgcccc aaccgtaaca ggcagtgcgg acgtcacgaa tcggatcgta
     6181 aagtgagaca aagattccat cgcacggtgc agagacaacg gcaaactcgc gcaaggcgcg
     6241 gaagatttgg agtaactcgc gcgcggtgcc gagtgcctgc gagaggatgg atacccgttc
     6301 cagcagtcgc tcatactctg aaagcgcgcg ttgatcagct tcgcgcaacg cctcttccgc
     6361 agcccggcgc gcggtgatgt cacgcgccac tccctgaatg ccaaccggct tgccggattt
     6421 gaaaatgaga cggctgctaa cttccagtcg caccaaacca ccgtgcttcg cctgcacatc
     6481 gatctcgtaa ttagtgcgct cttcgccggc gagttttcgc cccgtcattt cgcgtgcggc
     6541 cgcatccgat ccggcggtga gaaactgtct catgttcatc ttgcgcaatt cctcgcgcga
     6601 aaaacccgtc aacctttcta ctgcactgtt catggaagtg acattgccgt cgagatcaag
     6661 cgtgaacaca atgtcgttgg cgttttcaat cagctcacgg taacgctctt cactttcgcg
     6721 cagcgcttct tcggcccgtt cccgatcact gagagtcttc ctcaccttgc gttccgaaat
     6781 ttgcaattcc gaagctgtgc gttcggtggc cgcgcgcgct ttaacctggg agtgtgtcag
     6841 accaaacagt agcaagctga taagagcccc gacaactacg acgtagatct gggaattacc
     6901 tttcgcggca gcaaattccg gtctggatgt gaataccgac gtccaggtcc ggccggcaat
     6961 ctcgatgttg atctgggccc ggaatggccg gtaagcgctg ttattgatcg gcgccgcaac
     7021 cgtcgctgta tcgtgaatta gattttcacg cgtgggtact tgtccgtcgt ggaggctgat
     7081 atcaacatcg gtggtgttct tgatcgcgag gattgctttc aagaagtcag cagcgcgaaa
     7141 acggctaaac acaaagcccg caagtgcgtc tcgccgttct tcgtccgtgg tcggcgtgcg
     7201 atcattttcg tagattgggg cgtaaatcag gaaccccacg tcgcccggtt cttcaccgtc
     7261 ttttaccaat ggcaggtgcg ccgtagcggc gggcaagccg gtgtctcgcg cggcctccat
     7321 cgccgcacga cgcaccggtt cactgcccat gtcgaaacca accagctgat gtgggtggtt
     7381 atcgagcggc tcaaagtaga caaccggatt gtattcatcg cgctcctgtt cgggccagat
     7441 tgtgaaatca gaatgatggc ggctgcgagt ctccgagatg aacgatgctt tttgctgccg
     7501 tttcacccga gctatgtaac caatgccctg ggcaccaggg tattgatgct tcaactcgag
     7561 ccgctctaca aaattgtgaa actggctggc gcttgtcgtg ggactgatgg caaacaaacc
     7621 cgtgcccgcg cggagtacct cgatgtagat ccgggggcgt ccccgcagat acgtcgttag
     7681 ttcctgagtt gttgaaccga agcgaatgag atcttgagat ttggcagttc gccaaacata
     7741 aaaagtagag aaagctgtga tcaggaggcc gagcagaagc acaaaatacg gaaggaagta
     7801 ccggcgcggt cgaattccgt taagctttga ttcccctact ggcattggaa actacaacaa
     7861 cgagctactt gatccggctg gttcgtcgcc gaagaaaatc catgagaaca aactgcgaaa
     7921 gattgtccgg gttcgcgaag taagctttgc cattcgtcat ggcggtcatc tgcttcacaa
     7981 actctacgag ataatagtca ctcgccagca tgaaagtatt gatcattata cccgccttgc
     8041 ggcaggcctg gacttctttg aacgtttccg ccatcacaaa gggatcgccg cccattgaat
     8101 tcttatagat gcgacgcgtg acagtttggt cggggcgccg gccgcgtccc acgccggccc
     8161 ccgccagcat ttggttgcta tcaacgaaac aagccgtggg tttgccatcg gtaatcatca
     8221 cgatctgttt catttccttg cgttgcgact gaaggatacg ccgtgccaac cgcaagcctt
     8281 cggctgtgtt cgtgtgataa ggaccaacct gcgtggttgc cagctcagcg aacgccaatt
     8341 cttccgcgct gtcatggaag agcacgagct tcagcgaatc gcccggatac tgagtgcgaa
     8401 tcagatgtgc gagtgcaagt gccactttct tagccggcgt gaaacgatcc tcaccataaa
     8461 ggatcatcga gtgcgagcaa tcgagcataa ccacggtcgc acaggaagat tgataatcac
     8521 actggtgtac gtgcagatcg ctgtattcga gatttagtgg aacgcgcaga ccttctcgtt
     8581 ggatggccga caagagcgtt gtattcacat cgaggttgat ggtgtcgcca aactcataac
     8641 gcttgcttgc tgccgcggcc tctacaccgg tggacagctg gggcgtatcg tgtctgcccg
     8701 ctgagctctt gcccaggctt cccaacagat ttcgcaaggt cttgtaacca agaaagtcga
     8761 ggcctttttc ggtaacttca aacttgatgt tgcgcggcag gggctccgag acctgcgggc
     8821 cacccgctcc cggttgctgc gatctctcag caggcagttc ctgcagcttt agatagcctt
     8881 cttccaccag ccgctcaatc agttggttga gcatctcttc gagtgccgaa cctttaaact
     8941 cgccgtcatt gtccgcgagc atttcctgaa cctggtattc gttaagcagt ccctggttca
     9001 tgagcgcttg aagtagtgcg cgccgcagcg catccaacga ggtgtcgggg tcgtttcgct
     9061 ggcgcgtcca gtagtagtcg tcgttgtagc cagaagagag gagcgaatca gacaactctt
     9121 ccatcaagtc gccgaggtta attgcggaga catcgaggcc tttgaatttg gagtacttaa
     9181 tgaaccgcat caaaattgcc gactgccgat tgccaattgc cgattgccga ttagtgacta
     9241 gctattgccg gatgccaaat ctagagtagt tcaggaccaa cgctagtaac aaaagcttgg
     9301 aatttccact ccaattgcca atcgccaatc gccaatcacc actcagcaat cccttcagct
     9361 atacttccct gtctccgtca ggtcgtcgta gatcatcccg cgtcggtcct tcttcgcctt
     9421 cgtaactgct gtatagccac cttcctcgtt gcggctgatc ttattctcag cgtagagacc
     9481 tcccaacacc aattcgcaag ccgcgaccac tcgagccgta tcagctttat tgaaatcaaa
     9541 aggaatcaaa ctggtttcga tcaactcggg cacggcttcg agcagacgcc ggcactcgtc
     9601 agccgcagca gtatcagcca gtcttaagtt attcccggca tcgaaccatt gcaccgtgct
     9661 cgcgaattca atgcccacaa aataaccgtt gaatatttct ccagcggcgc gcttgattag
     9721 atctctggcg atgcgacggg caccgatctg ttcgccctcg tattccaatt ccatctttcc
     9781 ggtcatcgaa ggaatcgccg cgtaaatatc agagatccgc ggcacgatta ctttttcgcc
     9841 agtcagcaac gcgcggcgct cggcgttgga aatcactgac tccgtcaccg ttatcggcaa
     9901 gcgctgggaa acacccgact gtttgtccac gcgctgatca tcgcgcgcct cgaacgcaat
     9961 ctgttccacc acttcgcgga tgaactccgg aatgtgtagc tccgcttgcc aaccgtcacg
    10021 atcgacccag gcttcctgcc gggtgatctg aattgcagtc ggcaactcgg ccggataatg
    10081 tgtcgtaatc tcagcgccga tgcggtcctt cagaggggtg atgatctttc cgcgcgccgt
    10141 gtagtcttcc ggattagccg aaaagacgag cagcacatcg agcggcaaac gcactgggta
    10201 acccttaatc tgcacgtcgc cttcctgcat gatgttgaag agaccaacct gaatctttcc
    10261 ggcgagatcg ggtagttcat tgatggcgaa gatgccgcga ttcgctcgcg ggaggaggcc
    10321 gtaatgaatt gtcagctcat cgctgagcag atgaccgccc ttcgcggctt tgatgggatc
    10381 gacgtcgccg atgatgtcag cgatcgttac gtccggagtt gccagctttt cgacaaaacg
    10441 cgaatcgcgt tcgatccagg ctatcgggag cgagtcgccg tgagtttcca gctgttcgcg
    10501 accgtaggca gagattggct tgaacggatc gtcgttaatc tccgaaccgg caatgatggg
    10561 aattcgttcg tcgagcagct cagttagttg ccggatgatc cggctctttg cctgaccgcg
    10621 cagcccaagc aaaattatgt tgtgccgcgc taggatggcg ttgaccaatt ggggaataac
    10681 cgtttcatcg taaccgacga tgccgagaaa gagtttttct ccggccttaa gcttccggat
    10741 caggttggct cgcaattcgt ctttgacgga aacgctacgg tagccgctgg cttttaactc
    10801 acccaaagtc gctggttgtc gcattttgat tagtcccgat tggttcgatc tgatttacga
    10861 catcttaata cgggcaagtg aaagtcccta aaccggaggc atcagag
//
LOCUS       DASUEX010000213         4816 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1074764, whole genome shotgun sequence.
ACCESSION   DASUEX010000213 DASUEX010000000
VERSION     DASUEX010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4816
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1074764"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            343..930
                     /locus_tag="VFV61_03595"
     CDS             343..930
                     /locus_tag="VFV61_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899587.1"
                     /translation="MIRLLLVSSLAILSAASVLAQTEKTTPAPAATPMNKSQGTEITT
                     PAANPADVSSIDAIIAAVYDVISGPAGKKRDWDRMRSLFMPGARLIPTGPRQAGGYGS
                     RALTVEDYVTRGTPLFEKEGFFETERARRTESFGQIAHVFSTYESRHAPDDAKPFQRG
                     INSMQLMNDGKRWWIVTIFWQGEDEKNPLPEKYLK"
     gene            complement(1122..1940)
                     /locus_tag="VFV61_03600"
     CDS             complement(1122..1940)
                     /locus_tag="VFV61_03600"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC/CoxI family protein"
                     /protein_id="HEX4899588.1"
                     /translation="MSESANRPASAVVNAAILQLLERGETAALATVVAGSSAVGAKLL
                     VESTGETTGDLGSAALNNVVTQFAQQFLNSRDEAHNFSVSDLAPQLTSLAATRILFER
                     IQPEPRIVIFGAGHVGAALAKLASFAGYHVTLIDDRAELVMRERFPEELIELVAAESW
                     TKAASKAIGPGRGVSVAVVTRGHAEDEECLRAVMNSTPDYVGLIGSKRRTNIVLERLR
                     VTGVAEGKLASVCAPIGLDIGAVTPEEVALAILAEIVAVRRGGEGGSLSAFRRA"
     gene            complement(1965..2261)
                     /locus_tag="VFV61_03605"
     CDS             complement(1965..2261)
                     /locus_tag="VFV61_03605"
                     /inference="COORDINATES: protein motif:HMM:NF017897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF962 domain-containing protein"
                     /protein_id="HEX4899589.1"
                     /translation="MPESFVENYKAKHQHPLNKLTHSIGIPLIVLSLPLFFFNWRWAL
                     ALFIFGWILQFVGHAIEGNQPAFFRNPVYLLVGPLWLAKRAAAALGLVKTSSSK"
     gene            complement(2370..2708)
                     /locus_tag="VFV61_03610"
     CDS             complement(2370..2708)
                     /locus_tag="VFV61_03610"
                     /inference="COORDINATES: protein motif:HMM:NF014662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEX4899590.1"
                     /translation="MSSLSQPDLYRLIEAANERGECVVVATVAHTRGSTPQQRGAKML
                     FFEDGQAVGTVGGGCIEAEVWAEAREALRSKQSALHHYSLTAEEASEEGMVCGGTMEI
                     FIDCQLPIAD"
     gene            complement(2836..3588)
                     /locus_tag="VFV61_03615"
     CDS             complement(2836..3588)
                     /locus_tag="VFV61_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899591.1"
                     /translation="MQRFIRSVAVLVILVLVLGLSLIDAGAQTRRKRRSRRVPRPVIT
                     NPTITPSAETSTSGEPKVLSTADETPDEPVAETTKSRKKKAANNEEDMQQTINALSNQ
                     VNRLNDKLTQMQANDRSLLDMERLTRAEQRSESLRSQQLEAETKLADLQSQLDQIDYA
                     LKPENIERVTSGYGTTRPEEARDTRRRQLESEKSRLQAQVRILETSRTRLETAVANAD
                     AEVDMLRRRLQQHDATPTVVDSQPARNQKPER"
     gene            complement(3832..4686)
                     /gene="nadC"
                     /locus_tag="VFV61_03620"
     CDS             complement(3832..4686)
                     /gene="nadC"
                     /locus_tag="VFV61_03620"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEX4899592.1"
                     /translation="MNWMDEGALYSQIADMLREDLGRGDITSQAIVERNTRARGRFVA
                     NEKMVVAGLEAAEEVFLTLDSQQQLESFVADGEEIAAGKVIARTAGFADILLAGERVA
                     LTLMQRLSAVATSTGQFVHAVAGTGAQIVASRETTPGLRMLERYAVLLGGGKTNRFGL
                     DDGVVITSNHLAVTGGLAVALRHAKEKLGHAQKIQVQVSNEQELRTAVNHGADILIFE
                     SLSPEQVGKLTEVGRALSPSLSFECSGRMTAANARAYAEAGANVIRINLLQCSPAVMD
                     ISFVVRPF"
BASE COUNT         1088 a         1380 c         1248 g         1100 t
ORIGIN      
        1 ggagtcggtt agctgcagca ccagccaacc ctgagccacg gcctgcatcc aggtgccgat
       61 attggaaagg aaggcgccga cccaaaagcg cctgtagttc gcatgcgaaa gcgcacgaaa
      121 catcgccgta cgacgtttca tcggccgctc aagcggcgaa acctcctgta cctctaaaac
      181 cattctttgg ttggggactg ctcccttcta agcaccttgc tgactggttg ccggtaacat
      241 ttgatcatca caatgttagc gacgaacatt gcagcactgc cgcagcgata gaaatttgtg
      301 taacattcag gtctcgtcaa ttctaacagg ggggagattt ttatgatcag attgttactc
      361 gtcagctcgc ttgccatctt gagcgctgct tccgtgctgg cgcaaaccga gaaaaccaca
      421 ccagctcctg ctgcaacgcc catgaacaag tctcagggaa cggaaattac aactccggcg
      481 gctaaccctg ccgatgttag ttcaatcgat gcgatcatcg ccgcggtgta tgacgtgatt
      541 tcagggcctg ccgggaagaa gcgcgattgg gatcgcatgc gatccctctt catgcctggt
      601 gcacgcctga ttcccaccgg gccgcgccag gctggcggtt atggatcccg agcattaaca
      661 gtcgaggatt atgtcacgcg gggcacgccg ttgttcgaga aagaaggctt ttttgaaacg
      721 gaacgcgcgc gtcgcaccga gagtttcgga caaattgctc atgtcttcag cacttacgaa
      781 tcgcgtcacg ctcccgacga tgcgaagcct tttcaacgtg gcatcaacag catgcagttg
      841 atgaatgatg gcaagcgttg gtggattgtg acgatcttct ggcaaggcga agacgagaag
      901 aatcctttgc cggagaagta tctgaaatag cgaggagaga gcaacaaaca agaagggagg
      961 aagacatttt ccatatatca tttcaaattt ctcatttgtc attcgcgacg gttgtttctt
     1021 ttcttggatg taggtcctaa cggaacgaaa atccaaattg aattcgcgaa tgagaattga
     1081 aaaatgatat atggaaaatg tcttcctctt ctcctgttcc cctatgctct acgaaaggcg
     1141 gaaagcgagc cgccttcccc accacggcgc actgctacaa tttcagccag gattgctaac
     1201 gcaacctcct cgggggtaac agcgccgatg tccagaccta tcggtgcaca gacgctcgcc
     1261 agcttccctt ccgcaacacc ggtgacgcgc aaacgctcca ggacaatatt cgtgcgacgc
     1321 ttactcccaa tcaacccgac gtaatcaggt gtggagttca tcaccgcgcg caggcactct
     1381 tcgtcttccg catgcccgcg cgtgacaacg gccacggaaa cgcctcgtcc cggcccaata
     1441 gctttgcttg ctgccttcgt ccaactctcg gctgccacca actcaatcaa ctcttccgga
     1501 aagcgttcgc gcatcactaa ctcggcgcga tcgtcgatca gcgtgacgtg ataaccagca
     1561 aagctcgcca gcttagcgag agcggcgccc acgtgcccgg ccccaaaaat aacaattcgg
     1621 ggttcgggtt gaatccgctc gaaaaggatt cgcgtggccg ccagcgaagt taactgcggg
     1681 gccaggtcgc tgacgctgaa gttatgtgct tcatcgcgcg aatttaagaa ttgctgtgcg
     1741 aattgagtta cgacattgtt cagcgcggcg ctccccagat ctcctgtggt ttccccggtg
     1801 ctctctacta agagtttggc tcccactgcc gacgatcctg caaccacagt tgcaagcgct
     1861 gcagtttcgc cgcgctccag tagttgcagg atggcggcgt tgacgaccgc agaggcgggt
     1921 ctgttcgcgg attctgacat ggccgaaggg acgatacgtg aggactattt agaggatgat
     1981 gttttcacga gaccgagcgc ggcggccgca cgctttgcca accaaagtgg tcccaccagc
     2041 aaataaactg ggttcctaaa aaaggctggt tgattgcctt cgatggcatg accgacgaac
     2101 tgtagtatcc aaccgaagat gaagagcgcg agcgcccagc gccagttgaa aaagaacagc
     2161 gggagcgaaa gcacgatcag cggaattccg atgctgtggg tcaacttatt cagaggatgc
     2221 tgatgcttgg ctttgtagtt ttcaacaaat gattcgggca tgagacctcc ggtgattgcc
     2281 gattgccaat tggcaattag ttcttttgac ggtcagccca accggtcaca ttgaagagtg
     2341 aaattcaaca aaaggcaaat tgccaatctt caatcggcaa tcggcaattg gcagtctatg
     2401 aagatttcca tcgtgccgcc gcaaaccatt ccctcttcgc tcgcttcttc agccgttaat
     2461 gagtaatgat gcagcgccga ttgcttcgag cgcaatgctt cacgagcttc cgcccaaact
     2521 tcggcctcaa tgcaaccgcc accgactgtt cctaccgcct ggccatcttc gaagaagagc
     2581 attttcgccc cacgttgttg cggcgtcgaa ccacgcgtgt gcgccaccgt ggcgacgaca
     2641 acgcactcgc cacgttcgtt ggcggcttcg atcaatcggt agagatctgg ttggctgaga
     2701 gaactcatga aagggtccgt ggtcagttgt cagttttgag ttgtcaattg tcactagtta
     2761 gatccgacgt aacttctgct gcaacgaacc actggtaccg accactgaca actgaccact
     2821 aaccaatcgc caatcctacc gctccggctt ctgattgcgc gcaggttgag agtcaacaac
     2881 ggtcggcgtg gcatcgtgtt gctgcaaacg ccggcgcagc atgtccactt cagcgtcggc
     2941 attagcaact gctgtctcca aacgagtcct gctcgtttcc aggattctga cctgcgcctg
     3001 caagcgagac ttctcactct cgagctgtct ccggcgcgtg tcacgcgctt cctctggacg
     3061 agttgtgccg taacctgaag ttacgcgctc gatgttctca ggcttcaacg cataatcaat
     3121 ctgatcgagt tgggattgca ggtcagctaa ctttgtttca gcttcgagtt gctgcgaccg
     3181 caaagactcg gaacgctgct cggcccgcgt caatctttcc atatccagca gcgaccgatc
     3241 attcgcctgc atctgcgtca gcttgtcgtt cagccggtta acctggttgg agagcgcgtt
     3301 aattgtctgc tgcatatctt cttcattatt tgcagccttt ttctttcggg actttgtggt
     3361 ttcggcaacg ggctcgtctg gtgtttcgtc cgcggtgcta agtactttcg gttcaccgct
     3421 cgtagaagtt tctgcggacg gcgtgatcgt cggattggta ataaccggtc gcggaacacg
     3481 ccgcgagcga cgcttacggc gcgtctgcgc gccggcatca atcaagctca ggcctagaac
     3541 cagaaccaga atcactaaaa ccgcgaccga tcgaatgaat cgctgcatag aaactccctt
     3601 tccccttcga aaaccggctt tcaagatacc ctcggtgagg agatgcgttc aagcgctcgc
     3661 acgatgcttg agtgccccat taccaacgtc tggcaaccga tctcgttcca caaactcggc
     3721 gttgaagcga acctgcctcc gcaggtaagc agaactcgag gcgcaagcct atgccgcctc
     3781 gcatcaggcg ccagagccgc ttgagatggt gttccgccat ccagattcac ctcaaaaagg
     3841 ccgaactacg aaactaatgt ccatcaccgc gggcgagcat tgcagcaggt tgatgcggat
     3901 gacatttgcc cccgcttcgg cgtacgcgcg agcattcgcg gccgtcatcc ttccagaaca
     3961 ttcaaaactg agcgaaggcg agagcgcgcg gccaacttca gttaacttac ctacttgctc
     4021 gggtgacaaa ctttcaaaga tcagaatgtc ggcgccgtga tttaccgccg tccgcagctc
     4081 ctgctcgttg gaaacttgca cctgaatctt ttgggcgtgg cccagttttt ctttggcgtg
     4141 ccgcaacgcg actgccaagc ccccagtgac ggcaagatga ttagaggtga tcacaacacc
     4201 atcatcgagt ccaaaacgat tagttttgcc tccgcctaaa agcaccgcat agcgctccaa
     4261 catccgcaat ccgggcgtcg tttctcgcga ggcaacaatc tgagctccag ttccagccac
     4321 tgcgtgtacg aattgaccag tcgaggtagc taccgcggag agtcgctgca tcagcgttag
     4381 cgccactcgt tcgcccgcca gcaggatgtc tgcgaatcca gccgtacgcg cgatcacttt
     4441 tccggctgca atctcttcgc cgtcggcgac aaaggattcc agttgttgtt gtgaatcgag
     4501 ggtgagaaaa acttcttccg cagcctccaa gccagcgacg accattttct cattcgcgac
     4561 aaagcggccg cgcgcgcggg tgttgcgttc gacgatcgcc tgactagtga tgtcgccgcg
     4621 acccaggtcc tcccgcaaca tatctgcgat ctgggagtag agcgctcctt catccatcca
     4681 attcatggtt gatttcctga ctcttaacta agcgaagcga tgcggggttc ttggaaaaaa
     4741 tcatttctag tttccccaaa tggcgtgagc accagagggt agtccaccgt atggcgatag
     4801 actcgttggt ccgctc
//
LOCUS       DASUEX010000214         5640 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1167647, whole genome shotgun sequence.
ACCESSION   DASUEX010000214 DASUEX010000000
VERSION     DASUEX010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5640
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1167647"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..878)
                     /locus_tag="VFV61_03625"
     CDS             complement(<1..878)
                     /locus_tag="VFV61_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899593.1"
                     /translation="MKTRIKQLLRKRQVTLLGILGALTLLLACYPATEVSYSNPQDPG
                     AAQWLIEFKTGEERVHLEMRYQRKRDNGYGYSNNGFMTAPDKLTGLTREQAMSSGTNV
                     KFQLQRDAGTFYFEGWFKEGNGSGHFTFSPNTAFGAELNRLGLGTPTAEQQLSMALHD
                     VGFALINELKSQGYEQPTLEQLVKLGQHGVHLEYVQGLKSLGYSVKYIDFLIKMRDHG
                     VTVNFIRELASLNYTGLTAEELIRTRDHGVTGKYINEFRAAGYNQQTLDDWIMLRDHG
                     VNIEFVRELDSLGYSR"
     gene            1375..2463
                     /gene="tilS"
                     /locus_tag="VFV61_03630"
     CDS             1375..2463
                     /gene="tilS"
                     /locus_tag="VFV61_03630"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEX4899594.1"
                     /translation="MKTTTGKIRTRSLPPKLSRFARRLLSAWKQADFPRNNAAVIVAV
                     SGGADSTALLLALHELIRAGKLSLQLTVAHLDHGLRELSKEDAAWVKQLATGLGYPAV
                     VRRVDVHKRAAETGDNLEQSARRARYDFLKATARKARAQLVLTAHTVDDQAETVLLRL
                     LRGSAAEGLSGIESQRPLEQGSNIQLGRPLISWARRTEVEDYCRAEQIDFRRDEMNEN
                     EEFSRVRIRKQLLPLMQSFNNKIVDALSRTAGLLREDADFLSLEAQALLTAATVGSEL
                     NANGSASLNVRVVGAAPAAVRRRTLRLWLERERGHLRRLEMVHLVALDRLLTGDKGGR
                     VAQLPDGAQVTRKRGWLVLNGVSPRKKS"
     gene            2725..4674
                     /gene="ftsH"
                     /locus_tag="VFV61_03635"
     CDS             2725..4674
                     /gene="ftsH"
                     /locus_tag="VFV61_03635"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEX4899595.1"
                     /translation="MTLSSTARQIIFWLLIVAGALLLYKLVVNPTGRNSTAIDLSTLY
                     TKMEAREIKQLTVRQNETTAIDTRNQEYRVQLTNEQIKNELFKTANEPVDGKKLVEKF
                     EDDSGSSSYIWPMLITWAPLLFIIGIWVFMLRQMQSGGNKALSFGKSRAKLLNNQQKR
                     VTFKDVAGVEEAKEVLQEIIEFLKEPQKFQKLGGRIPKGVLMMGPPGTGKTLLARAIA
                     GEANVPFFSISGSDFVEMFVGVGASRVRDLFEQGKKNAPCIVFIDEIDAVGRHRGAGL
                     GGGHDEREQTLNQLLVEMDGFESNDGVILIASTNRPDVLDPALLRPGRFDRRVVVSRP
                     DVRGREGILKVHTRKIPLGEDVDISVIARGTPGFTGADLANLVNEAALNAARYNKKLV
                     AMADFELAKDKVLMGAERKSMVISNEEKRVTAYHEAGHTLVGLKVPNADPVHKVTIIP
                     RGMALGLTQQLPEGDRHNYSEEYLLGQIAILMGGRIAEESFLGSITTGASNDIERATE
                     LARAMVCEYGMSEMGPLTFGKKEEQIFLGREIAQHRDFSEETAIKIDEQVKKIVTAQY
                     QRARNIIEENRDVMVKLSECLLERETLDAVEIRRIVAGLPLDEQQPVSDPAGNDEDRP
                     QLKEPAAKPLKPILPPITGGNPAPA"
     gene            4796..>5640
                     /gene="folP"
                     /locus_tag="VFV61_03640"
     CDS             4796..>5640
                     /gene="folP"
                     /locus_tag="VFV61_03640"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEX4899596.1"
                     /translation="MHREWHIGGGRTLPIGKRTLVMGILNVTPDSFSDGSQFSSLDQA
                     LAHAEKMITEGADVIDVGGESTRPGAVPVTAEEEIERVVPVIAALANRTDIPISIDTT
                     KALVARAALAAGAAIVNDISALRFDFHIADEVAQAGAGLVLMHSRGTPITMHRLPPVA
                     DIMKEVISSLSSSITMAERRGVKRDSIVIDPGIGFGKSQEQNLELIAKLEQLAAAFPD
                     LPLLIGTSRKSFIGRLLADESGEPAPVSERLHGTLATIAIAASQGVSIVRTHDVKPAG
                     DVVRI"
BASE COUNT         1314 a         1520 c         1579 g         1227 t
ORIGIN      
        1 aatcgcgagt agcccagcga atccaactcc ctgacaaact ctatgttcac gccgtgatcg
       61 cgcaacatga tccagtcgtc caaagtctgt tggttgtaac cagccgcgcg aaactcatta
      121 atgtactttc cggtcacgcc gtgatcgcga gtgcggatca actcctccgc agtgagtccg
      181 gtgtaattca ggctcgcaag ttctcggatg aaattgaccg tgacgccgtg atctctcatc
      241 ttgatcaaaa agtcgatgta cttgacgctg taaccaagcg acttcaaccc ctgcacgtac
      301 tcgagatgaa caccatgttg acccagcttg accaactgct cgagggtcgg ttgctcatat
      361 ccttgagact taagttcatt gatcaaagcg aagccgacgt catgtagtgc cattgaaagc
      421 tgttgttcgg cggttggtgt accaaggcca aggcgattta gctctgcccc aaaggcagta
      481 ttgggactga aagtgaagtg acctgaacca ttaccttctt tgaaccagcc ttcgaagtaa
      541 aacgttcccg cgtcgcgctg aagttggaac ttcacgttgg tgccggacga cattgcctgc
      601 tcacgagtca gtcccgttaa cttgtccggg gcagtcatga aaccgttatt gctatagcca
      661 tacccgttgt cgcgtttgcg ctggtagcgc atctcgaggt gaacccgttc ctccccagtt
      721 ttaaattcga tcagccactg cgccgcacca ggatcctgcg ggtttgaata gctaacttct
      781 gttgccggat agcaggccag cagcagcgtc agggcgccga gaatccctaa aagcgtgact
      841 tgccgtttgc ggagaagttg cttgattcgc gttttcattg tttgaagtac gggataatcc
      901 atggcagcct cctgaaaatc ctaaaaacaa gcgtggtttg ggcggagcag tacggctaga
      961 caattcgggt tctgaaaaga taaacaccac gccgggtgcc tttgtgacac tgaaataatc
     1021 aagtccggct ctcgaggtta gtagtgcttt cgacgcgtag cgtcgcggtg atcttagccc
     1081 ggcttttcaa agccgggtct aacgacacgt aatccccgga gtcgcgtcag cgacggctga
     1141 cagatgaaaa cacccagcag gcgctaccag tcgtcgctac gcgacgaatt caaaaaggcg
     1201 tcgccgtccc ggcgttgaaa cgctgggcta aaaacattgc gacgcttcgc gtcgagagaa
     1261 tcagctctgt aacgtcgcgc gcgggcgatt gagggtcgca tacggaattg gtttgtcagt
     1321 tgcggaatcc cagtcagttg acgatgtctg aggtacgttt gacaatcagg cagattgaaa
     1381 acaacaaccg gcaaaatcag aacacgttcg ctgccaccaa aactcagccg ttttgcgcgc
     1441 cgcctactct ccgcctggaa gcaggcggac ttccccagga ataacgctgc cgtcattgtg
     1501 gctgtgtcgg gcggtgccga ctccactgcg cttttgctcg cgcttcacga actaatcagg
     1561 gccggcaagc tttcgttaca attaacagtt gcgcatcttg atcatggctt aagagagttg
     1621 agtaaggaag acgcggcctg ggtgaagcaa ctggctactg ggcttggcta tccggcagtt
     1681 gtgcggcgag tcgatgtcca caaacgcgct gctgaaacgg gcgacaatct cgaacagtca
     1741 gctcgccgcg ctcgctacga tttcctgaaa gcaactgccc gcaaagcccg cgcgcagtta
     1801 gtgctgacgg cccatacggt cgatgatcag gctgagacag ttttgcttcg ccttttgcgc
     1861 ggcagcgccg ccgagggttt gagcggcatc gagtctcagc gccctttgga gcagggctcg
     1921 aatattcagc tcggcagacc acttatttcc tgggcgcggc gcacggaagt ggaggactac
     1981 tgccgcgcag agcagatcga ctttcgccgc gacgagatga atgagaatga ggagttctct
     2041 cgggtcagga tccggaaaca actgctaccg ctaatgcagt ccttcaataa caagattgtg
     2101 gatgcacttt cccgtaccgc cgggctcctg cgcgaagatg cagactttct ttcacttgag
     2161 gctcaagcgc tccttacggc cgctactgtt ggttctgaac tcaacgcgaa cggctctgct
     2221 tctttaaacg taagagtcgt gggtgcggcg cctgcggcag tgagacggcg gactctgcga
     2281 ctatggctgg agcgagagcg gggtcacctc cgccgcctcg agatggtgca cctggttgct
     2341 ctggaccgtc tgctgacggg agataagggc ggacgggtcg ctcagttgcc ggatggcgca
     2401 caagtgacac gaaaacgcgg ctggctagtg ctaaatggcg tgtctccccg caaaaagagt
     2461 tgaaaaacat gggagcactc tctagaatcg atgtcgctaa caccgccgca gcaagagctg
     2521 gcggttttcg ctttttcggc cagcagcaac ggacgaagca tttgacgaga gttggcccta
     2581 acataactaa acggttagca caaccgccat tggtcgcgcg gcatcaaaac gagtcgctta
     2641 gaagcgacgt ttgcaatttc ccggcggcag ggctgagaaa gacaaaggac gcgttctggg
     2701 ttatgatcag cgtcggaggc tttaatgaca ttgagttcca ctgctagaca aataattttt
     2761 tggttgctga tagtcgcggg tgcactcctg ctctacaaac tggtggtcaa tccgacgggc
     2821 cgcaattcca cggctatcga cttgtccacg ctgtacacaa agatggaagc ccgtgagatc
     2881 aagcagctga ctgttcgaca aaatgagact actgcgatcg acacgcgcaa tcaggaatat
     2941 cgcgtacaac ttactaacga gcaaattaag aacgagttat ttaagacggc gaatgagccg
     3001 gtagacggca agaagctcgt cgaaaaattt gaagacgact cgggatccag cagttatatc
     3061 tggccgatgc tgatcacctg ggcgccgctg cttttcatca tcggcatttg ggttttcatg
     3121 ctgcggcaaa tgcagtcggg aggcaacaaa gcgcttagct ttggcaaatc gcgggctaag
     3181 ttgctcaata accagcaaaa gcgggttacc ttcaaggatg tcgctggtgt tgaagaagcc
     3241 aaggaagtac tgcaggaaat cattgaattc ttgaaagaac cgcaaaagtt ccaaaaactc
     3301 ggcggtcgca tacccaaagg cgtgttgatg atgggcccgc cgggaaccgg aaagactttg
     3361 ctggcacgcg ctattgctgg tgaagccaac gttccgttct tctcgatctc aggttctgat
     3421 ttcgtggaga tgttcgtggg tgtgggcgcg tcgcgcgtcc gcgacttgtt cgagcaaggc
     3481 aagaagaacg ctccgtgtat tgtcttcatc gatgagattg atgctgtcgg ccggcatcgt
     3541 ggcgctggtc tgggcggtgg acacgacgag cgtgaacaga ccttgaatca gttgctggtt
     3601 gagatggacg gcttcgagtc gaacgacggt gtcattttga tcgcttcgac gaaccgtccc
     3661 gacgtactcg atccggcgtt gcttcgtcca ggcagatttg accgccgtgt agttgtgtcg
     3721 cgacccgatg ttcgcggccg cgaaggcatt ctcaaagttc atacgcgcaa gattccgctc
     3781 ggcgaagatg tggacatcag cgtgatcgcg cgaggcacac ccggattcac cggagccgac
     3841 ctggctaatc tagttaacga agcggcactc aacgctgctc gctataacaa gaagctggtg
     3901 gccatggcgg actttgagct ggcgaaggac aaggtgttga tgggggccga gcgcaagagc
     3961 atggtcattt ccaacgaaga gaagcgtgtc accgcttatc acgaagcggg acatactctc
     4021 gtcggcttga aagtacccaa cgccgatccg gtgcacaagg tcaccatcat tccacgcggc
     4081 atggcattgg gactgacgca gcaattgccg gaaggcgatc ggcataacta cagcgaagaa
     4141 tacctgctgg gccaaatcgc aatcctgatg ggcggccgca tcgccgaaga gtctttcctc
     4201 ggcagcatta cgacaggcgc ttccaacgac atcgaacgcg caactgaact ggcgcgcgcg
     4261 atggtttgtg aatacggcat gtcggagatg ggtccgctca ctttcggtaa gaaggaagag
     4321 caaatcttcc tcggtcgcga gatcgcacag caccgtgact tttcggaaga gactgcaatc
     4381 aagatcgacg agcaagtaaa gaagatcgtc accgcacaat accagcgcgc gcgaaacatc
     4441 atcgaagaga atcgcgatgt gatggttaag ctctcggaat gcctgctcga acgcgagacg
     4501 ctggatgccg ttgagattcg ccgcatcgtt gccgggttac cgctggatga acagcagccg
     4561 gtgtctgatc cagctggaaa tgacgaggat cggcctcaac tgaaagaacc ggcagcgaag
     4621 ccgctgaagc ccatcctgcc gccaattact ggaggcaatc cggctccggc gtaaatcggg
     4681 aataacctaa aaagtgaaaa ggggaatggg gcaacccgtt cctctttttc ttttccacct
     4741 tcttcccccc gttccccctt ttaccctctt cccctgttcc cctatcatca atgccatgca
     4801 tcgtgaatgg cacatcggcg gcgggagaac tcttcccatc ggcaaaagaa ccctcgtgat
     4861 gggcatcctg aacgtcaccc cagacagctt cagcgatggc tcgcaattct cttccctcga
     4921 tcaagccctg gcacatgctg aaaagatgat cactgaagga gccgacgtca tcgacgtggg
     4981 cggagagtcc acgcgtccgg gcgctgttcc ggttactgcg gaagaagaaa ttgagcgtgt
     5041 cgtaccagtc atcgcagcct tagctaaccg caccgacatc ccgatctcga tcgatacaac
     5101 gaaggcttta gttgcgcgtg cagctcttgc agccggcgcc gcaatcgtca acgacatttc
     5161 cgccctgcgt tttgattttc atatcgccga tgaggtggct caggcaggtg caggtttggt
     5221 cttgatgcat tcgcgcggca cgcccattac catgcatcgc ctgccaccgg ttgccgacat
     5281 catgaaagag gtaatcagca gtctgagtag cagtatcaca atggccgaac gacgtggcgt
     5341 aaaacgagac tcaatcgtca tcgatcccgg gatcggcttt ggcaaaagcc aggagcaaaa
     5401 ccttgaattg atcgcaaagc tggagcagct ggccgccgcc ttcccggacc ttcctttgtt
     5461 aattggtaca tcgcgaaagt ctttcatcgg ccgtctgctc gccgatgaaa gtggcgaacc
     5521 ggcgcctgtc tccgaacgcc tccatggcac tctcgcaact attgccattg ccgcctcgca
     5581 gggagtgagt attgtgcgaa cgcacgatgt gaagccggca ggcgacgtcg tacgtattat
//
LOCUS       DASUEX010000215         3919 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1412072, whole genome shotgun sequence.
ACCESSION   DASUEX010000215 DASUEX010000000
VERSION     DASUEX010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3919
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1412072"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..1352)
                     /locus_tag="VFV61_03645"
     CDS             complement(87..1352)
                     /locus_tag="VFV61_03645"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HEX4899597.1"
                     /translation="MFSRLERLRRVVITGMGCVTPIGIGRAAFWRGLISGESGVRRIT
                     SFDIENSPVKIAAEIEGFDWEAQLNPKDRKHVPRTVPLALAAAREALEDARLTPTELT
                     LDQRREFGVVLGTGGGGLAFTERQYEYWYIGPANKASVYTIPASTHGGLSSELSMAFG
                     LRGLSHIVSTGCTSSTDALAYAAEHIALGRQNVMIAGGVDAPIAPGILAAFNLMTVLT
                     SEWNEEPQRASRPFSRNRSGIVLGEGAWLYVLEDLEHAQARDAKIYAEIIGYGATCDA
                     YHRVRLEEGGDEPARAMRLALEDAGCRPEDVDYVNLHGTSTLLNDRIETSALKLAFDG
                     HAKRIPMSATKSQVGHPQGASGAAGLGAALCAMHEKMIPPTINLDEPDPDCDLDYVAN
                     DARAADVRIALCNCIGFGSKNSALLVARM"
     gene            complement(1517..2575)
                     /locus_tag="VFV61_03650"
     CDS             complement(1517..2575)
                     /locus_tag="VFV61_03650"
                     /inference="COORDINATES: protein motif:HMM:NF024813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEX4899598.1"
                     /translation="MGIMDAIRSQFIEVIEWLDDSGNTLLYRFPVQGQEIKNGARLTV
                     RESQAAVFVFQGQIADVFSPGLYTIDGGNTPILSKLGAWMHGFNSPFKAEVYFVNTRQ
                     FTDLKWGTANPVMMRDTDFGMVRLRAFGIYSIRVTEPRAFIKEVAGTNAHFVTEDIEG
                     QLKRTLVSGFSDALAESKIAALDLASNYDELSKFVRSKLGDEFKSYGLELTKYLIENI
                     SLPQEVEAAMDKRTSMGVIGDVGRYTQFQAADAMRDAAQNTSGGAGTGAGLGAGFAIG
                     NAMAGAMTNAMNPKPADSSAGAAKVNCIKCGALMLAGSKFCSECGAKQEKQKCSKCQS
                     ELSPGTKFCNECGTKVEG"
     gene            complement(2718..3212)
                     /locus_tag="VFV61_03655"
     CDS             complement(2718..3212)
                     /locus_tag="VFV61_03655"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEX4899599.1"
                     /translation="MTEQSGQSWRSLQTRIWRYAPLLFWMAFIFFASTGAFSGDNTSR
                     IIRPLLLWLFPHVSEERIAIVHLLVRKLAHFSEYAVLAFLAARAFAGSARRMLREHWV
                     VASLILVVAYALLDEFHQSFVASRTASIFDSLIDITGGLFMLICLAHFKRTRMKKEFT
                     RRAS"
     gene            complement(3307..>3919)
                     /gene="atpG"
                     /locus_tag="VFV61_03660"
     CDS             complement(3307..>3919)
                     /gene="atpG"
                     /locus_tag="VFV61_03660"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HEX4899600.1"
                     /translation="FNTNLIKAAQAFVREHAGKTIEIVAVGRKGRDFFRRRGALNGEY
                     IGLTGKGRVDFAEAIGVARDVINRFTEDPELDKAFVIYNEFKSVLQQRVVVEQLLPVS
                     RAKTESEANLSSQPVSLVDYIYEQPPAEMFSQLLPRLIETQIFRALLESVASEQGARM
                     TAMDSASKNASELIDSLTLNMNRVRQAAITNEIIEVVSGAAAQ"
BASE COUNT          911 a         1108 c          998 g          902 t
ORIGIN      
        1 ggtgtcgggt gacagcgtca ggtttcgggt ttcaagcgtt cgtcgcccgt cacctgatcc
       61 ccgacacctg atccccatca ccggtttcac atccttgcaa ctaacaaagc gctgttcttg
      121 gaaccgaaac cgatgcagtt gcaaagcgcg attctcacgt cggccgcacg tgcgtcatta
      181 gccacataat ccagatcgca gtccggatcg ggctcgtcaa ggttaatcgt tggcggaatc
      241 atcttctcgt gcatcgcaca tagtgcagcc cctaatccgg ccgcaccgct ggctccctgt
      301 ggatgaccca cttgggactt agttgctgac atcggaatgc gcttagcgtg gccatcaaac
      361 gccaatttaa gagcgctcgt ttcgatgcgg tcgtttagca gtgtcgatgt tccatgcaga
      421 ttcacgtaat ccacgtcttc cgggcggcag ccagcatcct ctaacgctag gcgcatagct
      481 cgcgctggct catcgccacc ttcctcgagc cgtacgcgat ggtaggcatc gcaagtagca
      541 ccgtagccga ttatttcggc gtagatcttc gcgtcacgtg cttgggcgtg ttctagatct
      601 tcgagaacgt agagccaggc gccctctcct aaaacaatgc cggatcgatt gcgtgagaat
      661 ggtcgcgacg ctcgctgcgg ctcttcattc cattcactgg tgagtacagt catcaggttg
      721 aatgcggcga gaattcctgg agcaatcggt gcatcaacac cgccagcaat cattacattc
      781 tgacgtccca gcgcaatgtg ttctgctgca taagcgagcg cgtcagtaga gctggtgcaa
      841 ccagtggaaa cgatgtgcga taaaccgcgg aggccgaacg ccatcgaaag ttcactcgac
      901 agaccaccgt gagttgacgc gggaatcgta tagacgctcg ctttgtttgc agggccgatg
      961 taccagtatt catattgccg ttcagtaaat gccaagccgc cgccgcccgt tccgagtacg
     1021 acgccaaatt cacgacgctg atctaacgtg agttcagttg gcgtcaaccg agcatcctcc
     1081 agcgcctcgc gggcggccgc gagcgccagc ggaacagtgc gtggtacgtg tttgcgatct
     1141 ttaggattaa gttgagcttc ccaatcgaag ccctcgattt ccgcggcaat cttgaccggc
     1201 gagttctcga tgtcgaatga ggtaatccgg cgcacgccgc tttcgccact aatcagcccg
     1261 cgccaaaatg cggctctgcc aatgccaatc ggcgtaacac agcccatccc cgtaatcact
     1321 acgcggcgca gtctttccaa ccggctaaac atttcgcttt ctccggtacg gcatcggatt
     1381 atttcggggg tcgctacgag gataacattg ctggcgcgca aaacaacggg atcggaaaca
     1441 agcaaagggt cgctcgtctc tctggcaagc gacccttcgg ggtttcccga tctacaactt
     1501 agcgttggaa gcgattctag ccttccactt tagtcccgca ttcattgcag aacttggttc
     1561 cgggtgacaa ttcactctga catttggaac acttttgctt ctcttgcttc gcgccacact
     1621 cgctacagaa tttgctgccg gcgagcatca gcgcgccaca cttaatgcag ttaacctttg
     1681 cagcgccggc tgaagagtca gcgggcttcg gattcatcgc gttggtcatc gcaccagcca
     1741 tcgcattgcc aatcgcgaat ccggcgccca ggcccgcgcc ggttcccgct ccgcccgaag
     1801 tgttctgggc tgcatctcgc atcgcatccg ccgcctgaaa ttgtgtatag cgccccacgt
     1861 cgccaattac gcccatcgag gtccgcttgt ccatcgccgc ttcgacttcc tgcggcagcg
     1921 aaatgttttc aattaggtat ttcgtcagtt ccaacccgta cgacttgaac tcatcaccca
     1981 atttactgcg cacgaatttg cttagctcat cgtaattaga agcaagatca agcgccgcga
     2041 tctttgattc cgccagggcg tcgctaaatc cactcacgag agtgcgctta agttggcctt
     2101 cgatatcttc ggtaacgaag tgtgcattcg ttcccgctac ttccttaatg aaagcccgcg
     2161 gctcggtgac gcggattgaa taaatcccga aggcacgcag tcgcaccatc ccaaaatcgg
     2221 tgtctcgcat catcacggga tttgcggttc cccatttgag atctgtgaat tgcctcgtgt
     2281 ttacgaaata aacctcggct ttgaatggcg agttgaagcc atgcatccag gcgccgagtt
     2341 tagaaagtat cggagtatta ccgccatcaa ttgtgtaaag tcccgggcta aaaacgtcgg
     2401 caatctgtcc ttgaaaaaca aagacagcgg cctgcgactc gcgcaccgtt agccgcgcgc
     2461 cgtttttgat ctcctgaccc tgaacgggaa agcggtacag cagggtgttg ccagagtcgt
     2521 caagccactc aatgacttca ataaattgtg aacgtatggc atccataatt cccatagttt
     2581 gaaatctcct tcgtaaagca gttcttctgc aggatctcga tcagcctaac ctgtaacttc
     2641 tgaacaggac cggcagttac taacatcctc aatattgcac aaagcggccg tccggggaaa
     2701 gcaggataga accaacctca tgacgcccgc cgagtaaact cctttttcat ccgcgtacgc
     2761 ttgaagtggg caagacaaat cagcatgaaa agaccgccgg tgatgtcaat caggctgtcg
     2821 aagatcgatg cagtacgtga agcaacaaag ctttggtgaa attcgtcgag cagtgcatag
     2881 gcaacgacca ggataaggct tgctacgacc cagtgttcac gcagcattcg tcgcgcggag
     2941 ccggcaaaag cgcgtgcggc aaggaaagcc aacacagcat actcactaaa atgagccaac
     3001 ttccgaacca gaaggtgaac gatcgcgatc cgttcttcgc tgacatgtgg gaaaagccaa
     3061 agaagcaggg gtcgtattat gcgcgaggta ttgtctcccg aaaatgcgcc ggttgaggcg
     3121 aagaatatga acgccatcca gaaaagcagc ggcgcataac gccagatccg ggtttggagg
     3181 gagcgccagg actgcccgga ttgttcagtc atcaggattt gaggtgagcg gttgcggcaa
     3241 atctagaata atgggaagcc gcataagcag gcttcccatt aggtgcagag atgagcgcgt
     3301 cactttttac tgagcggctg cgcccgagac aacttcgata atctcgttgg taatcgccgc
     3361 ctgccttacg cgattcatat tcaaggttaa ggaatcaatc agttcgctgg cgttcttgga
     3421 agcggaatcc atagccgtca tgcgtgcacc ttgctcggaa gcaactgatt caagcagcgc
     3481 tcggaagatc tgcgtctcaa ttaaacgcgg caacagctga ctgaacatct cagccggtgg
     3541 ttgttcatat atgtaatcaa cgagtgaaac tggctgagac gagagattcg cttcgctttc
     3601 agttttggca cgcgaaacgg gcaacagttg ttcaactact actcgctgct gtagcactga
     3661 cttgaattca ttgtaaatca cgaatgcttt gtccagttcc ggatcctcag taaagcggtt
     3721 gattacatca cgggctacgc caatagcctc cgcaaaatcg acgcgcccct tgcccgtaag
     3781 cccgatgtat tccccattca gcgctccgcg acggcggaag aagtctcttc ctttccgtcc
     3841 caccgcaacg atctcaatcg tctttcccgc atgctctcga acaaaggctt gagcggcttt
     3901 gattagattc gtattgaac
//
LOCUS       DASUEX010000216         5167 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_616982, whole genome shotgun sequence.
ACCESSION   DASUEX010000216 DASUEX010000000
VERSION     DASUEX010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5167
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_616982"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1205
                     /locus_tag="VFV61_03665"
     CDS             <1..1205
                     /locus_tag="VFV61_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899601.1"
                     /translation="EQGYDDQKKRVYALLEKLTGQEKSYSEYARTLANLLAMRKESFA
                     PGKLKIEDLIAKNAMGERNSIHELQNYIVGEAPGGLQLRADNSLDLEKSFVRVDYFAL
                     LQNVTVKNNVQPAVVNRPIDMVAHRIPTIQLKHLLNEANLTSDVIWVNGGPDKQALIM
                     AREDSQGDLSFRYLPVKRLTQDADGSVHFNELAWQPDLPLHIFEDEQLVIPPGQDRAT
                     WLSQWHTDLDWLHALHRTKYANGLVGLYEELAHHKNPRLDLDEPNISPNERLSRRFIK
                     RQRDLAEADLLIVANNHWNFDVRGFNPGGNHGSFLRVSTHSTLMLAGGDRTQLPRGLV
                     VDEPYDSLSFMPTMLALTGNLRDDKTPLPVLWSKGFRRFPGRLVQEVLPATPGIQRIA
                     VTGASPTH"
     gene            1205..3418
                     /locus_tag="VFV61_03670"
     CDS             1205..3418
                     /locus_tag="VFV61_03670"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HEX4899602.1"
                     /translation="MASDAELSSDWQDAEIDELEEDPEPPGSSGARRSFIWLQALAFL
                     LGIALLTYVINRVGVQPIFDALFRVGFGFFFVIGISGMRHFLRTIAMRAAVPSEHRRI
                     SFTHAFAARLGGEAISFLTFTGPLLGEATKVALLRKRVPLTYGVPALVVDNLIYNLSV
                     IFFVLTGACVMLFAYQLPPQVHYLLIAIAVVAALGIVIAGFAAKRRILLLTWIIDRLA
                     QLRLSPKVILKRRQHIYHLESKVYDFYKHQPKAFFGMIVCNLVAHLTSVVEVYVTLRM
                     LGFNPLVSQAYIIESLTKVINVTFGFVPGTIGVYEGGTEVILQALGFAQATGVALALV
                     RKAAILFWTSIGLLVLTWRTAPNAWGRLLDRSPRLRTFMDSLVLSNIAHRPARTAVSI
                     LGTAVGVLLIVFTVGLSHGVLHERGRRESNIGAEIMVRASGTLGFGGSSPFVLPVSRA
                     TEIATISGVRAATPLGQALDRSDAGFGTRLLDGINFDEYSRLTGMRMREGVPLTGGDQ
                     AIVDPVWQQNRRALVGSTVQIYERPFKIVGVYEPPGGGRVKIPLATMQEQEGGEGKTS
                     AILVACSDPAQQETVAARIRERFPDDQIIFTRDLPELYASGVPALNIFIKVVVGVAAA
                     ISMLVILLAMYTTVTERTRQIGILKALGMSKPAIAWVIEKEALLVSFLGVAVGVLLTV
                     VARFFVMRVTSLTIEIEPQWILISLLVGLFGGSLGALYPALRAARQDAVDALSYE"
     gene            3521..3745
                     /locus_tag="VFV61_03675"
     CDS             3521..3745
                     /locus_tag="VFV61_03675"
                     /inference="COORDINATES: protein motif:HMM:NF018961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HEX4899603.1"
                     /translation="MSIARITEISSTSSKSFEDAIQSGVTRACRTLRNVRSAWVKEQQ
                     IRIDKDTIVEYQVNLMITFVLDEDNEDKVA"
     gene            4054..5046
                     /gene="bla"
                     /locus_tag="VFV61_03680"
     CDS             4054..5046
                     /gene="bla"
                     /locus_tag="VFV61_03680"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF033103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase"
                     /protein_id="HEX4899604.1"
                     /translation="MNSKIFSLQSPLAAVVLCFSLVACNNLPDAESSLASTTPKPTRP
                     LPVACSSPSLRSQIEKIAARTGGPVGVAVGLVDGGEVVASNGQQHFPMQSVYKLPIGM
                     AVLQQIDKGTLKLEQTISIKPIEYVRIGQHSPIRDKHPRGAELSLAQILKYAVSDSDG
                     TASDVLMRVAGGAAVIRKYLQELGIDGIMVLDTEKEIGKSVEVQYRNWATPEAALALL
                     KVVAEGRSLSPQSQKLLSELMTETPTGPKRIKGLLPPGTLVAHKTGTGLGGGLTRAVN
                     DIGLVTLPDGKRLAIAVFVSDTKLNSEACEAVIAEISRAAWDCWTKPEVSSPAS"
BASE COUNT         1103 a         1406 c         1451 g         1207 t
ORIGIN      
        1 tcgaacaagg ctacgacgat caaaaaaagc gggtctatgc gctactcgaa aaactaacag
       61 gtcaggagaa gagttacagc gagtatgccc gcacgctcgc taacctgctg gcaatgagaa
      121 aggagagttt cgctcccggt aaactcaaga ttgaggatct gatagctaag aatgccatgg
      181 gcgagcgcaa cagtattcat gaactacaga actacatcgt tggcgaagcg ccgggcggtt
      241 tgcagttgcg ggcggacaac tcgcttgatc tggaaaagag ttttgttcgc gtagactact
      301 tcgcactgct gcagaacgtt actgtcaaga acaatgttca gcccgcggtt gttaaccggc
      361 ccatcgatat ggtggctcac agaattccaa cgatacaact caagcacttg ttaaatgaag
      421 cgaacctcac ctcggatgta atctgggtca atggcggtcc tgacaaacag gctctgatca
      481 tggcgcgtga agattcacag ggtgacctga gctttcgtta cctgccagtc aaaagattga
      541 cccaggacgc cgatggcagt gttcacttta atgaacttgc gtggcagccc gatttaccgc
      601 tgcacatctt cgaagacgag caactggtca tccctcccgg ccaggatcgc gccacctggc
      661 tcagtcaatg gcatacggat ctggattggc tgcatgcgtt gcaccggact aaatacgcta
      721 atggactcgt cggcttgtat gaagaacttg ctcatcacaa gaacccgcgc cttgatctcg
      781 acgagcccaa cattagtccc aacgaaaggc tgagccgtcg ctttattaag cgccaaagag
      841 acctggctga agccgatctg ttgattgttg ctaacaacca ttggaacttt gacgtacgtg
      901 gttttaatcc gggcggtaat cacggctcgt ttctgcgagt gtcgacacac tcgaccctga
      961 tgttagcggg cggcgacaga actcaattgc cgcgcggcct ggtcgtggac gagccgtatg
     1021 acagtcttag ctttatgccg accatgctgg ccttaacagg aaatttgcgg gatgataaga
     1081 ctccccttcc ggttttgtgg agcaaaggct ttcgccgatt cccgggacgt ctggtgcagg
     1141 aagttttacc agccacgccg gggattcaac gaatcgcagt tacgggagca agtccgacgc
     1201 actgatggct tctgatgcag aattgagttc tgattggcag gatgccgaga tcgacgaact
     1261 cgaagaagat cccgagccgc ctggttccag cggcgcccga cgttcattca tttggctgca
     1321 agccctcgcc ttcctgctgg ggattgctct cctcacctac gtcatcaatc gtgtcggtgt
     1381 acagccgatc ttcgatgctc tcttccgcgt cgggttcggt ttcttttttg taatcggcat
     1441 cagcggcatg cggcatttcc tgcgcaccat tgccatgaga gcggcggtcc ccagcgaaca
     1501 ccgtcgcatc agttttactc acgcattcgc ggctcgcctc ggcggcgaag caattagttt
     1561 tttgaccttt acgggaccat tactcggcga agcaacgaag gtggccctgc tccgcaaacg
     1621 ggtgccgctc acttacggcg ttcccgccct cgtagtcgat aacctcattt acaacctgtc
     1681 ggtaatcttt tttgttttga ccggcgcctg tgtgatgttg tttgcctacc agctaccgcc
     1741 gcaggttcac tatctgttga tcgcaattgc ggtcgtggcg gcactcggga ttgtaatcgc
     1801 cggatttgct gccaagcgcc ggattctgct gctgacctgg atcatcgacc ggctggcgca
     1861 attgcggctc agtcctaaag tgattctgaa acgacggcag cacatctatc atctcgaatc
     1921 caaagtctac gacttttaca aacatcagcc gaaagccttc tttggaatga tcgtctgtaa
     1981 tctggtggcg catctgacga gtgttgttga ggtctacgtc acgctgcgca tgcttggatt
     2041 caacccgctc gtttcccagg catatataat cgagtcgctg acgaaagtta ttaatgtcac
     2101 ctttggtttt gtccccggca ccatcggagt ctacgaaggc ggcactgaag tcatcttgca
     2161 ggcgctcggt tttgcccagg cgactggtgt ggcactcgcg ctcgtacgca aggccgcaat
     2221 tcttttttgg acctctatcg gcttgctggt gctgacctgg cgcacggcgc cgaacgcctg
     2281 gggtcgcctg cttgatcgca gcccgcgttt gagaactttt atggacagtc tcgtactatc
     2341 aaacattgca catcgcccgg cccgcaccgc agtgagcatt ctcggcacag ccgttggcgt
     2401 gctcttgatt gtgttcactg tgggactctc gcatggagtg cttcacgaaa ggggtcgccg
     2461 cgaatcgaat attggcgccg agataatggt gcgcgcttcg ggcaccctgg gctttggagg
     2521 ctcctcgcca tttgtgcttc cggtctcacg ggctactgag attgctacga tttccggcgt
     2581 ccgtgccgcc acgccgttgg gccaggcact cgatcgcagc gacgcgggct ttggcacgcg
     2641 cttgctcgac ggtatcaatt ttgacgaata ctctcgcctg accggaatgc gcatgcggga
     2701 aggcgtgccg ctcacaggcg gcgatcaggc gatcgtcgat cccgtctggc agcaaaaccg
     2761 gcgcgccctg gttggttcca cagtgcaaat ctatgaaagg cctttcaaga ttgtcggagt
     2821 ctatgaaccg cccggcggag gtcgcgtaaa gatcccgctc gcgactatgc aagaacagga
     2881 gggtggcgaa ggtaagactt ccgccatcct ggttgcttgc agcgatccag cacaacagga
     2941 gactgtcgcg gctcgcattc gcgagcgctt ccccgacgac cagattattt tcacgcgcga
     3001 cttgcccgag ctttatgctt ccggtgtccc ggcactgaac atcttcatca aggtcgtggt
     3061 cggagtcgcc gcggcaatca gcatgctcgt aatcctgcta gcgatgtaca cgacggtaac
     3121 cgagcgaaca cgacaaattg gcatcttgaa ggcgctcggc atgtcgaagc cggccatcgc
     3181 ctgggtgatc gaaaaagagg cgttgctcgt cagctttctc ggcgttgctg tcggagtgct
     3241 acttacggtg gtggcccgtt tctttgtgat gcgcgttacc agcctgacga tcgagatcga
     3301 gccacaatgg attctgattt ctcttctggt gggattgttc ggcggcagtc tcggagctct
     3361 ctacccagcc ttacgtgctg cgcgccagga cgcggttgat gctttgagtt acgagtgagt
     3421 ggcacgaagc aaagttttct ttccccccgt tgcccccctt ttcttaatcg agcccacttt
     3481 agacgggcga tcaccagcca tttttaggag aaatgaacac atgtcaatcg cacgtattac
     3541 tgaaatcagt tcaacctcga gcaagagttt cgaggatgca attcaatcgg gtgtaactcg
     3601 cgcctgccga acgctgcgta acgtccgcag cgcctgggtc aaggaacagc agattcgtat
     3661 cgataaagat acgatcgtgg agtatcaagt taatttgatg attacgttcg tgctcgacga
     3721 agacaacgaa gacaaggttg cctagcagga gcctgcacgg ctgcgctttt ctgccgccgt
     3781 gcagctacct cgaaacggct tcgccgccgg acgtgcggcg ccgactctgc ggcgccgact
     3841 ctccggcgcc ggagagttag attggaaatc cgggctttgc ccttggtttg ggctcggtcc
     3901 tcagaaatta tctggggctg acgcagcccc aagctaaaca agggttttca ggcgcgcccc
     3961 acatccggcg gcaaagccga accgatgctg gctcaccact cttggtgccg tgcccttaaa
     4021 gtcgtcgttc tacactacca agtagagtca ttcatgaact ccaaaatatt cagtttgcag
     4081 tcgccgttag ctgctgtcgt gttgtgtttt tccctggtcg cgtgtaacaa cctccctgac
     4141 gcagagtcct cgctagcatc gacaacgcca aagccgacca ggccactgcc cgtagcttgt
     4201 tcatctcctt cactccgctc acaaatcgaa aagattgcgg cacgaacggg agggccggtt
     4261 ggtgtggcgg tgggtttggt agacgggggt gaagtcgttg ccagcaacgg tcaacagcat
     4321 ttcccgatgc agagcgttta caagttgccg atcggcatgg ctgtgttgca gcagatcgac
     4381 aagggcacgt tgaagcttga gcaaacgatc agcatcaaac cgattgagta tgtgcgcatt
     4441 gggcagcata gcccgatccg cgacaagcat ccgcgcggcg ccgagctgtc cctcgcccag
     4501 atattgaaat acgcggtttc cgatagcgac ggcacggctt ctgacgtgtt aatgcgagtt
     4561 gccggcggcg ccgcggtaat acgaaagtac ctgcaggagc tgggtatcga cgggatcatg
     4621 gttctcgata ccgaaaagga aatcgggaaa agtgttgagg tgcagtatcg taattgggcg
     4681 acaccagagg cggctctagc cctcttgaaa gttgtggcgg aagggcgcag tctgtctccg
     4741 cagagtcaaa agttgttgtc ggaattaatg acagaaacac ccacagggcc gaaacgcatc
     4801 aaaggtttgc tgccgcccgg gacccttgtg gcacacaaga ctggtacggg attgggcgga
     4861 ggattgacgc gggcggttaa tgatattggg ttggtcacgc tgccggatgg gaaacgttta
     4921 gccatcgcag tcttcgtatc agacacgaaa ttgaattcgg aggcctgtga ggcggtaatt
     4981 gccgagattt ctcgtgcggc ctgggactgt tggacgaagc cggaggtaag ttccccagcg
     5041 tcctgatttc ctgaggagtc aaaaagatga tggctagcac ttcacgtctc tttgtaattg
     5101 cctgctccct gctcctgggc tgcggcatgg cgtttaattt gaaatccgcg gaagaccaaa
     5161 ccacacc
//
LOCUS       DASUEX010000217         3138 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_846305, whole genome shotgun sequence.
ACCESSION   DASUEX010000217 DASUEX010000000
VERSION     DASUEX010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3138
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_846305"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..797
                     /locus_tag="VFV61_03685"
     CDS             <1..797
                     /locus_tag="VFV61_03685"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX4899605.1"
                     /translation="SQEFPEVRFLNIARERLTYAELRAAGIKESQGRIVAITEDHCTP
                     SPDWCEQIARAHEAPYAAIGGAVEKRVPDSPLNWAFYLADYLRYMKPIPAAPSHTLTD
                     CNVSYKRSALDAIATVWESEFHEPNVHAALHARGETLWLEPQIVVYQQRSLDLMSAIR
                     DRYAFGRLFASTRFIKLTLAQRLLYSLLTLALPVVLVARVAGHILRKRRFLGEFVRAL
                     PYLILISTVWAGGEFLGYLTGTAEESLSPRSQSSESVSHGGQEAVI"
     gene            794..1828
                     /locus_tag="VFV61_03690"
     CDS             794..1828
                     /locus_tag="VFV61_03690"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX4899606.1"
                     /translation="MIFKGVEICCPHCKGELREVKAEERTLECESCYRSFPVIFEIPD
                     LRVFPDPYIDSEADRAKGLQVASRFEQSDFAALVDYYYSITSAVPVADAKRYTAGLLA
                     GVARAEGSLISWEKSTTGNTRAASLLEIGCGTAPFMVAAASSFKRVVGVDIAFRWLVV
                     AKRRLADAGLDLPLICACAEALPFPDEIFDRVVADSTLEHVTDQPLALHECHRVMAAP
                     GYLFVSTPNRFSIGPDPQVGLWSGSLLPERWLAAYVRRQGGIPPKRHLLSARKLSAMI
                     RQAGFAPPRVMLPDIGKKQRSNFGSGAQLLIDLYQTAKQMPLSRQALELVGPLLHAVA
                     AKQDGVGLRA"
     gene            1876..>3138
                     /locus_tag="VFV61_03695"
     CDS             1876..>3138
                     /locus_tag="VFV61_03695"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HEX4899607.1"
                     /translation="MNPFRNIFRLSVGDFVAKSLNFLAFVYLARTLGVEGYGVLEFAL
                     AILTYFLLFADGGLELWATREVASGTDLRSLAARIVPLRFVLALASFCGLIGFLLVLP
                     NYPGLRTVMILFGLTLFTQAVNLKWVFMGREEMPRVAIGLIIAQVVFAVAILVLVRSP
                     RTILWVPVLRLTSDLAMAVYFGRLFVKLNGDLRLRFTLRKAGMALRSALPLGASHGLA
                     FMSYNFDTILLGLLLTPAAVGLYSAAYKPVTVVLAMPTTYFIGLFPTLARTYTQDRDS
                     FSAIANRSFGLAALCAVPLGIGGLFLASPIINFLFGPAYTSAVPVLQILVWSAALVIL
                     RGTFRQSLNGAGRAGLDLRCAGASVLLNITLNLLLIPRYGIIGAAVATLVSEVLWFSL
                     AYYYFNRYISRLKLLPLLHQPVMAAIVMG"
BASE COUNT          606 a          872 c          876 g          784 t
ORIGIN      
        1 tcagccagga atttcctgag gtgcgtttct taaacatcgc ccgagaacga ctaacctatg
       61 ctgaattgcg agcagcaggg atcaaagaat cgcaaggacg aatcgtcgca atcactgaag
      121 accactgtac gccgagtcct gactggtgtg agcaaatagc acgcgcacac gaggcgccct
      181 atgcggctat tggcggtgcg gtcgaaaagc gagttccgga ctcacctctc aactgggcgt
      241 tctacctggc tgactatctg cgttacatga agccaattcc ggcggcaccg tcgcatacct
      301 tgacggactg taatgtctct tacaaacgat ctgcgctgga cgccattgct accgtatggg
      361 aatccgaatt tcatgaacct aacgtgcacg cagcattgca cgcgaggggc gagactcttt
      421 ggcttgaacc acaaatcgtg gtctaccagc agcgaagtct cgatctgatg tcagccattc
      481 gtgatcgcta cgctttcgga cgcttgttcg caagtacgcg cttcatcaaa ttgaccctcg
      541 cgcaacgctt gctctactcg ctactgacgc tggccttgcc agttgttctg gtggcgcgcg
      601 ttgcgggcca catcctccgc aaacgtcggt tccttggtga gtttgtacga gccttgccct
      661 atctaatcct gatttcgaca gtctgggcgg gaggagagtt tctgggttat ttgactggaa
      721 cggccgaaga gtccttgagt cccagatctc agagtagcga gagtgtaagc cacggtggtc
      781 aagaggcagt catatgatct ttaagggcgt cgagatctgc tgtcctcact gcaagggcga
      841 acttcgcgaa gtaaaggcag aagagagaac cctggaatgc gagtcttgtt accgcagttt
      901 tccagtcatc tttgaaatcc cggatcttcg agtttttccc gacccttata ttgatagcga
      961 agccgatcgc gcaaaaggat tgcaggtcgc aagccgtttc gaacaatcag acttcgccgc
     1021 actcgttgat tattactaca gtatcacttc cgcggttccg gttgccgatg cgaagcgcta
     1081 cacagccggg ttgttggctg gtgtggccag agcagaggga tcgttaatct cctgggaaaa
     1141 atcgactacc ggaaatacgc gagcggcgag tctgctggaa ataggatgcg gcacggcccc
     1201 cttcatggtg gcggccgcaa gctcattcaa gcgcgtggtg ggagtggata tagcttttcg
     1261 ttggttggtg gtggcaaaga gacggctggc cgacgccggc ctggatcttc ccctgatatg
     1321 tgcttgtgcg gaggccttgc cttttccgga cgagatcttt gatcgcgtcg tcgcagactc
     1381 aactctggag cacgtcaccg accaaccgct cgcgttgcac gagtgtcatc gggtaatggc
     1441 ggctccaggg taccttttcg tgtcaacgcc aaatcggttc agtattgggc cagaccccca
     1501 agtgggactc tggagtggca gtctgcttcc ggagcgctgg ttagctgctt atgtgcgtag
     1561 acagggaggc atccctccaa agcgacacct gctttcggct cgcaagctat ccgcaatgat
     1621 tcgccaagca ggcttcgcgc cgcctcgcgt gatgctgccg gatatcggca agaaacaacg
     1681 cagcaatttt ggcagcggtg cgcagctgct aatcgatctc tatcaaaccg ccaaacagat
     1741 gccgctcagt cgccaagcct tagagttggt cggaccactg ttacatgcgg tagccgcaaa
     1801 acaagacggt gtcgggctga gggcttaatt cttccgactg actgtgaatc gtcagcagcc
     1861 aaatcaaaat tctctgtgaa cccttttcga aacatctttc ggctctcagt cggcgacttc
     1921 gtggcaaaga gtctgaactt cctggccttt gtgtacctgg cgcggacgct tggcgtcgag
     1981 ggttatggag tgctggagtt tgcgctcgcg atcctgacgt actttctact tttcgccgat
     2041 ggcgggctcg agctttgggc cacgcgtgag gttgccagtg gcacagacct acgttcgttg
     2101 gctgcccgca tcgtcccgct gcgattcgtt cttgctctgg cgtccttttg cggattgatt
     2161 ggcttcttgc tagtgctgcc gaattacccg ggactgcgaa cagtcatgat cctgttcggc
     2221 ctcacacttt ttacgcaggc cgttaatttg aaatgggtgt tcatgggcag ggaagagatg
     2281 ccgcgcgtcg ccattggttt gatcatcgcg caggtcgtct ttgcagtcgc gatactagtc
     2341 ttggtgcgca gtcctcgaac aatcctttgg gtgccagtat tacggctgac cagcgacctg
     2401 gcgatggctg tctacttcgg cagactgttt gtgaagctca atggtgacct gaggctccgg
     2461 tttactttgc gtaaggcggg catggccctg cggtcggcac ttcctctcgg ggcgtcgcat
     2521 ggcctggcat tcatgagtta caacttcgat acgatcctgt tgggcttgct actaacgcca
     2581 gctgcagtcg gcttgtacag cgcggcctac aagcccgtta cagtggtcct ggcgatgccg
     2641 accacctact tcatcggact ttttcccacg ttagcgcgca cgtacactca agaccgcgat
     2701 tcgttcagcg cgatcgcgaa ccgctctttt ggattggcag ctctgtgcgc cgtgccgctc
     2761 ggcatcggcg gcttgttcct ggcgtctccg attatcaatt tcctttttgg accagcttac
     2821 acgagcgctg tacccgttct gcaaatactt gtatggtctg ccgccctggt aatcctgcgc
     2881 ggtacttttc gtcaatcact gaatggcgcc ggccgcgccg gtctggacct gcgctgcgcc
     2941 ggtgcttctg tcctgcttaa tatcaccctc aacctcttgc ttattccacg ctacgggatc
     3001 atcggggctg ccgtagccac tctcgtttcg gaagtgctct ggttctcgct ggcttattac
     3061 tatttcaatc ggtacatctc gcgtctgaaa ctgttgccgc tgctacacca gcccgtaatg
     3121 gcagccatcg tgatgggc
//
LOCUS       DASUEX010000218         2465 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_44768, whole genome shotgun sequence.
ACCESSION   DASUEX010000218 DASUEX010000000
VERSION     DASUEX010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2465
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44768"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="VFV61_03700"
     CDS             complement(<1..656)
                     /locus_tag="VFV61_03700"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEX4899608.1"
                     /translation="MQTSFSRRHLLGIRDLSAGEISHLLDTAETFRDVSRREIKKVPA
                     LRGRTVINLFFEPSTRTRTSFEIAAKRLSADAINISVSTSSVSKGETLLDTARNLEAM
                     APDCIVVRHSMAGAPNHLARMCNAPIINAGDGSHEHPTQALLDALTIREYKGRIEGLN
                     VAIIGDILHSRVARSNIYLLTKLGANVRVAGPGTLVPAEFGELVEQGLHVERRIEDAI
                     "
     gene            complement(863..1432)
                     /gene="pyrR"
                     /locus_tag="VFV61_03705"
     CDS             complement(863..1432)
                     /gene="pyrR"
                     /locus_tag="VFV61_03705"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF003549.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEX4899609.1"
                     /translation="MEFIEKARIMDGPRINRALARLASEIVEENHGAGDLYLVGIRRR
                     GVPLAERMADKIADLEGGRPPVGVVDITLYRDDLSTVGAKPIVNRTELPGDIESRNIV
                     LIDDVLYTGRTIRAALDQLIDFGRPRRVQLAVLIDRGKEHRELPIQADYIGKLVPTKK
                     SEIIKVMLTDFDEEEGVVIVERPPAEAVS"
     gene            1570..>2465
                     /locus_tag="VFV61_03710"
     CDS             1570..>2465
                     /locus_tag="VFV61_03710"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF003652.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HEX4899610.1"
                     /translation="MLYRSLLRRLLFRLPPETAHEIALQTLARAPRLTKLLAGGHDDK
                     ISFGKLERFGLSFSNPVGLAAGFDKDGIALQGLAALGFGFIEAGTVTFHPQPGNERPR
                     LFRLPADQALINRAGFNNKGAAAFAQRVGPAQEHHRDYVLGVSIGKSKVARLDDAIED
                     YLKSFTIVYPVADYVAVNVSSPNTPGLRDLQQANQLEALLLALQRRNRELRTQSKRFK
                     ELPLLVKISPDVSAAELEQIAAVAHKTAIAGIIATNTTIRRDGLRSSAAEIHACGDGG
                     LSGLPLRVRSSRMVARLYQLTAG"
BASE COUNT          553 a          687 c          657 g          568 t
ORIGIN      
        1 tcgattgcgt cttcgattct tcgctcgacg tgtaggccct gctcaaccag ttcaccgaac
       61 tcggccggta ccagagtgcc cggtcctgcg actctcacat tagctcccag tttggtcagc
      121 aggtaaatgt tggaacgagc aacgcgcgaa tgaaggatgt cgccgatgat ggcaacattt
      181 aatccctcga tgcggccctt gtattcccgg atcgtcaacg catcgagcag cgcctgcgtt
      241 ggatgttcgt gagagccgtc gccggcatta atgatagggg cattacacat tcgggcgaga
      301 tggttggggg cgccggccat tgaatgccgc acgacgatgc aatcgggagc catggcctcc
      361 aggttccgtg cggtatcaag aagcgtttcg cctttggaaa cgctggacgt ggacacactg
      421 atattaatgg cgtcagcgga caatcgtttg gcggcgattt caaacgaggt acgcgtccgg
      481 gtcgagggtt caaagaagag gttaatcacc gtgcggccac gcaatgccgg cactttcttt
      541 atctcgcgcc gtgaaacatc gcggaaggtc tcggccgtgt cgagcagatg cgaaatttcg
      601 cccgcggaca ggtcgcgaat tcccaggaga tgtctcctac taaaagatgt ttgcattact
      661 ttacgccttg gcctggatca ttacgagctt tgtacttagt gctttgtact tagttcgctt
      721 ttcagccttg agcggttttg gaacgaacta ttcactcggg ttacgaacaa agtacaaagt
      781 tccgagcaca aagttctaaa cctccaacac gcagagtcac agtcctagtg cgaatataaa
      841 aaaagcggcc ccgttggccg cttcatgaaa ccgcttctgc cggtggtcgt tcgacaatga
      901 ccactccttc ctcttcatcg aaatctgtca gcatcacttt tatgatttcg cttttcttgg
      961 tcggcacgag tttgccaatg taatcggcct gaatcggcag ttcgcgatgc tccttgccac
     1021 gatcgattag caccgccaac tgcacgcggc gcgggcgtcc aaagtcgatt aactgatcca
     1081 gtgcggcgcg aattgtgcgc cccgtgtaga gcacatcatc gataaggacg atgttgcgac
     1141 tctcgatgtc tccgggaagt tctgtgcgat tgacaattgg cttggcgccc accgttgaaa
     1201 ggtcgtctcg atagagtgtg atgtccacaa ctcccaccgg cggccggcca ccctcgaggt
     1261 cggcaatttt atcggccatt ctttctgcga gcggcacgcc gcggcgcctg atgccaacca
     1321 ggtagagatc gccagcacca tgattttcct cgacgatctc ggaagcgagc cgggcgagcg
     1381 cgcgattgat tcgcggccca tccataatgc gcgccttctc gatgaattcc atttcgcgcg
     1441 atactaacaa gaacaccggc gagtgacaag tgacacatca ccagtcacaa gttagaatgc
     1501 cttgctttta cttatttgct cattactggt catcgaatgc agcttgccaa ctgtgactag
     1561 ccaatcttaa tgctctatcg ctcattgctg cgccggctac tttttcgtct accgccggag
     1621 acggcgcatg aaatagcact tcaaacactt gccagggcgc cgcgtctcac caaacttctc
     1681 gccggcggtc acgacgacaa gatctcattc ggaaagctcg agcgttttgg tttgagtttt
     1741 tcaaatccgg taggtctcgc tgcgggattt gataaagacg gaatcgcgct gcaaggtctc
     1801 gcggccctgg gttttggttt tatcgaagcg ggtacagtga cgtttcaccc gcaaccggga
     1861 aatgagcgcc cccgtttatt ccgcttgcca gctgaccagg cgctaataaa tcgagcaggc
     1921 ttcaacaaca aaggtgcggc cgcgtttgct caaagagtcg gccccgctca ggaacaccat
     1981 cgcgactatg tcttaggcgt cagcatcggc aagagcaaag tcgcgaggct cgatgacgca
     2041 atcgaagatt atctaaagag ctttacaatc gtttacccgg tggcggatta cgtagccgtt
     2101 aatgttagtt cgccgaacac gccaggcttg agggatctgc aacaggcaaa tcagctcgag
     2161 gcactgctgc ttgccttgca acggcgcaat agggagttgc gcacacagag caagcggttc
     2221 aaagaactcc cgctcctggt caaaatctct cccgacgtct ctgccgctga acttgaacaa
     2281 atcgcggcgg tcgcccacaa aacagctatc gccggaatca tcgccacaaa cactaccatc
     2341 aggcgggacg gattaagatc gtcggccgcg gaaattcacg cttgcggtga cggcggctta
     2401 agcgggttac cattgcgcgt ccggtcaagc aggatggtag ccagactcta ccaacttact
     2461 gccgg
//
LOCUS       DASUEX010000219         2002 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_259863, whole genome shotgun sequence.
ACCESSION   DASUEX010000219 DASUEX010000000
VERSION     DASUEX010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259863"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1421)
                     /locus_tag="VFV61_03715"
     CDS             complement(<1..1421)
                     /locus_tag="VFV61_03715"
                     /inference="COORDINATES: protein motif:HMM:NF036516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899611.1"
                     /translation="MKNSHLKPGFRLLLAIQLLTTFSIAAAQQAPLRSINYRLSMPLP
                     ASHLFEVKVEVELPEQTSPPSLDFQMPRWSPGRYAVFDFAKNVQEVYASSGICPPKQD
                     VVAQTVCRPVTLPVSRIDNQTWRVETKGNSAITFSYKVFGNDLSGTFSQLDTRHANYN
                     GGSIFMYIVDHKADPVKLTITPPGVWRIINGRMERSEQREWQFPNWDIMIDTPTEIAP
                     DWTEDIFQVNGKKYHVMVHSFGDEGGKRSALVRDIEKIVRAETAMWGDPEFAEYTFLI
                     HFAADDRSGDGMEHLTSTQIIEPGALADPETYESTLGTVAHEFFHVWNVKRLRPAEFG
                     PWDFTRPANTRGLWVAEGLTNYYGHLMLHRAGIWNEAQFLRRESDTITGIESALGSRL
                     MSAEDSSQSAPFMDDAAHSQRTNLQNTSISYYPKGELIGLVMDLLIRGRSNGKASLDD
                     VMRRMYDEFYLKSPNASYYLRGRG"
     gene            1666..>2002
                     /locus_tag="VFV61_03720"
     CDS             1666..>2002
                     /locus_tag="VFV61_03720"
                     /inference="COORDINATES: protein motif:HMM:NF013570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HEX4899612.1"
                     /translation="MPATERLYYHDSHLIEFEARVIDQTERVSGWTAITLDRTAFYPT
                     GGGQPSDTGTIDGIRVVECIDDEANGILHVIQGRAPAVGSTVQGRVDWPRRLDHMQQH
                     TGQHILSQAF"
BASE COUNT          467 a          560 c          518 g          457 t
ORIGIN      
        1 cctcgaccac gcaggtagta gcttgcgttc ggactcttca aatagaattc gtcgtacatc
       61 cgccgcatta cgtcgtcaag cgaggccttg ccgttactgc gaccgcgaat aagcaggtcc
      121 atgaccaaac caatgagttc acctttcgga tagtagctga tcgaagtgtt ctgtaagttt
      181 gttcgttgcg aatgggccgc atcgtccatg aaaggagctg attgcgacga gtcttccgcg
      241 ctcattagcc gactgccgag cgcgctctcg attccagtaa tggtatcgga ctcacgccgg
      301 aggaactgtg cttcattcca aattcccgca cggtgcaaca tcaggtgacc gtagtaattg
      361 gtcaatccct ctgctaccca caaaccgcgc gtgttagccg ggcgcgtgaa atcccaggga
      421 ccaaactctg ctggacgtag acgcttcaca ttccagacat gaaagaactc gtgcgcaact
      481 gtgcccaacg tactttcata ggtttccggg tcagcgaggg cgccgggttc aatgatctgc
      541 gtcgaggtca gatgttccat tccgtcgcct gaacgatcat cggccgcaaa gtgaattaag
      601 aaggtgtact cagcaaactc cggatcgccc cacatcgcag tttccgcgcg gacgattttc
      661 tcaatgtcgc gcacgagagc gctgcgcttg ccaccctcgt caccaaacga gtgcaccatc
      721 acgtgatact tcttgccgtt cacttgaaaa atgtcttcag tccaatcagg tgcaatctcg
      781 gtcggcgtat cgatcattat gtcccagttc ggaaactgcc actcgcgttg ttccgagcgt
      841 tccatccgtc cgttgatgat tcgccagacg ccaggaggcg tgatcgtcag cttcactgga
      901 tcagctttgt gatcgacgat gtacatgaag atcgagccgc cgttgtagtt ggcgtgacgc
      961 gtgtccaatt gagaaaacgt gcccgaaaga tcgtttccga aaactttata gctgaaggta
     1021 atcgcagagt tacctttcgt ttcgacccgc caggtttgat tgtcgattcg gctgaccgga
     1081 agggtcacag gacgacaaac cgtctgcgct actacatcct gtttcggcgg acagattccc
     1141 gaactggcgt agacctcctg cacattcttt gcgaaatcaa aaactgcata gcgtcccggg
     1201 gaccagcgcg gcatctgaaa atctagtgac ggtgggcttg tttgttcagg taattcaact
     1261 tcaaccttca cctcgaataa atgcgaagcc ggaagcggca ttgaaagtcg atagttgatc
     1321 gagcgcagcg gcgcttgttg tgcagcggca atagagaatg ttgtcagaag ttgtattgcc
     1381 aacaacagac gaaagccagg tttcagatga gagttcttca ttagagtaag ttggatcctt
     1441 gggtttgatt gtcgggagac agcgtcggat tatgacatcg acatcgagtc tcaacaaaac
     1501 gcgttgcaag ctcgtacagc tccgcctctc ttggcgactt aacggttcgc tttcatcaag
     1561 gctgtgttgg tgaaatgatc ggttggtgct cgccgctcct tgcatgtcct cgaaccttgg
     1621 actaacttca aagatcccaa gccctaagac caaagacgaa taccaatgcc cgcaaccgag
     1681 cgtctctact atcacgactc ccacctgatc gaattcgaag cacgagtcat cgatcagacc
     1741 gagcgtgtga gcggctggac agcgatcaca ctagatcgca ccgcgttcta tcccaccggc
     1801 ggcggccagc ccagcgatac cggtacgatt gacggaatcc gcgtggtcga gtgtattgac
     1861 gacgaagcga acggcatcct ccatgtaatc caaggccgcg caccagcagt cggttccacc
     1921 gtgcaaggcc gcgtcgactg gccgcggcga ctggaccaca tgcagcaaca caccgggcag
     1981 cacattctct cccaagcctt cg
//
LOCUS       DASUEX010000220         2918 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1412889, whole genome shotgun sequence.
ACCESSION   DASUEX010000220 DASUEX010000000
VERSION     DASUEX010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1412889"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(36..851)
                     /locus_tag="VFV61_03725"
     CDS             complement(36..851)
                     /locus_tag="VFV61_03725"
                     /inference="COORDINATES: protein motif:HMM:NF022784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3142 domain-containing protein"
                     /protein_id="HEX4899613.1"
                     /translation="MPRYPRLRSALCVFSLLGVLLLFAVTVVYTRRDRSNFNPALASG
                     SDKLTKLPRTILWAWERPEQLDFLDTTETTVAFLAKTIQLRGARVVARPRLQPLKVPP
                     GAPLIAVARIESDPIEHPALTSAQLHETAREIAELSGQPNVVAVQIDFDATLSERAFY
                     RNLLFELRAQLRAGTALSITALASWCKGDNWLEELPIDEAVPMLFRMGIDKHQVLSQL
                     NEGEPFTSSKCRQSAGVSTDEPIARLPATQRLYVFNPRSWSPTSVGALLQKKL"
     gene            1155..2864
                     /locus_tag="VFV61_03730"
     CDS             1155..2864
                     /locus_tag="VFV61_03730"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF017038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HEX4899614.1"
                     /translation="MERETLEMDVVFVGAGPANLSGALHLTRLVKEHNQLVQEGKRQG
                     QPLGDIEIGVIEKGASVGAHILSGAVMDPKALKELIPDFLEQGAPLESPVKDDYFLYL
                     TEKHAIRSPITPPPLKNHGYYIVSLNRLTEWLGEKCEEAGVNIFPEFPGAEMIYDEND
                     AVVGVRTGDKGIDKEGKPKANFEPGVDLRAKVTVLGEGPRGSLTKRLSERLGLNEGRE
                     PQVYSIGVKELWELPDDRSPAGRVTHTLGFPSDAATYGGGWIYGLQNRILNIGYVTGL
                     DYSDPLIDPHAEFQRFKSHPHIARILEGGKMIRYGAKTIAAGGYFAMPQIYADGVLLV
                     GDCAGFLNSQRLKGIHSAIKSGMLAAEAIFEALQAGDFSAKQLKRYRELVDQSWIIPE
                     LRKVRNFHAGFKHGRWLGLINTGLQFVTGGKSWGLFDRAEAEPGHEVMKKLSSYGYKD
                     DRLEQRYDGLRFDGKITFNKLTDVYHAAVGHNEDQPAHLHVLDTNICSTRCAEEYGNP
                     CQRFCPAAVYEMVDDGGNGRRKLQINFSNCVHCKTCDIMDPYQIINWVTPEGGGGPDY
                     KGM"
BASE COUNT          713 a          701 c          844 g          660 t
ORIGIN      
        1 tgaaacaagc ttgcggagtt tagctgcact taggctcata gttttttctg tagtaaggca
       61 ccaacagaag ttggagacca ggaccgcgga ttgaaaacat agagtcgctg agtggccggg
      121 agcctggcga taggttcatc agtggaaacg ccggcgcttt gccggcactt gctcgacgta
      181 aaaggttcac cctcattgag ttgtgaaaga acctggtgct tgtcgattcc catgcgaaat
      241 agcatgggca ccgcttcatc tatcggcagt tcttccagcc aattgtcgcc tttgcaccat
      301 gaagcaagcg ccgtaatcga tagagctgtt ccggcgcgca gttgagcgcg cagctcgaaa
      361 agcaggttgc gataaaaggc acgttcgctt aacgtcgcgt caaagtcgat ctggaccgcg
      421 actacgttgg gttgacctga gagttcagca atctcacgcg cagtttcgtg gagctgtgca
      481 gaagtaagcg ccggatgttc aataggatcg gattctattc gcgcgaccgc aattaagggt
      541 gctcccggcg ggaccttcaa aggttgcaag cgcggacgcg cgaccacacg tgccccacgc
      601 aattggattg tcttcgcgag aaatgcaacg gtcgtttctg tagtatccag aaaatcgagt
      661 tgttccggtc gttcccaagc ccaaagaatc gtccgaggaa gtttggtcaa cttatcgctt
      721 ccactggcca aagcgggatt gaagttgctg cggtcacgtc tcgtgtaaac gaccgttaca
      781 gcaaacagga gtaagactcc cagcagggaa aaaacgcaga gcgctgaacg aaggcgggga
      841 taccttggca tacgatttag tcggcgaagt cgagagttta cactagcgac aagccctgcg
      901 cgataggtta ctgaggggtt ggggttgagg gtcaggttga atatcaaggt ctcgttccga
      961 tccgaatccc tcttggcata atctgcgtcg atgtgtctct gcggtttctt gctaagcctg
     1021 acttggatca gcagcggctg caattagaaa accactgacc gaccatcctt aacagtcgga
     1081 caatcagatt tgttatactc ccaaaaaatg aatggccatt catttctgca acttccaatt
     1141 ggaaaggtga attgatggaa cgagaaaccc tcgagatgga cgtcgtgttt gtgggagcgg
     1201 gacctgcaaa ccttagcggt gcgctacacc tgacacggtt ggtgaaagaa cataaccagt
     1261 tggtccagga aggaaagcgc cagggccaac cgctcggtga cattgagatt ggcgtaatcg
     1321 aaaagggcgc gtctgtcgga gctcatatcc tctctggtgc agtgatggat ccgaaggcgc
     1381 tcaaagagtt gatccctgat tttctcgaac aaggcgcgcc actggaatca ccggtcaagg
     1441 acgactactt tctttatctg actgaaaagc acgcaatccg ttcacccata actccacctc
     1501 cactcaagaa tcacggctac tacatcgttt cattgaatcg tcttacggaa tggttgggag
     1561 agaagtgtga agaagccggc gtcaacatct ttccggagtt tccgggcgcg gaaatgattt
     1621 acgacgaaaa tgacgcagtg gttggcgttc gcaccggcga caaagggatc gataaagagg
     1681 gaaaaccaaa agcgaacttt gaaccgggcg ttgacctccg ggcgaaagtg acagtgcttg
     1741 gcgaaggacc gcgcgggtct ctcaccaaac gtctgagtga gcgattgggc ctcaatgagg
     1801 gtcgtgaacc gcaggtttac agcattggtg taaaagagtt gtgggaactc cccgatgacc
     1861 gctctccggc cgggcgcgtg acgcatacgt tagggtttcc ttcggatgca gccacctatg
     1921 gcggcggctg gatctatggc ctgcaaaacc ggattttaaa catcggctac gttactggtc
     1981 tcgactatag cgatccgttg atagatccgc atgccgagtt tcagcggttt aagtcgcacc
     2041 ctcatattgc gcgcatactc gaaggcggca agatgattcg ctacggcgct aagacgattg
     2101 cggcgggagg ctatttcgcg atgccgcaga tctatgcaga tggtgtattg ctggtcggtg
     2161 attgtgccgg gttcttgaac tcacaacggc tgaaaggaat tcattcggcc atcaagagcg
     2221 gcatgcttgc cgccgaagcg atctttgagg ctttgcaggc gggagatttt tcggcaaaac
     2281 agttgaagcg ctaccgggaa ctggtggatc aaagttggat tattccggaa cttcgtaagg
     2341 ttcgtaactt tcacgccggc tttaagcatg ggcgatggct cggcctgatt aacacgggac
     2401 tgcaatttgt gacgggtggt aaatcctggg gactctttga tcgcgctgag gctgaacccg
     2461 ggcatgaggt gatgaaaaaa ctctcatcgt atggctacaa ggacgatcgt ctggaacagc
     2521 gttacgatgg gctgcgtttc gacggaaaga tcaccttcaa taagctgaca gacgtctatc
     2581 acgcggctgt gggccataac gaagaccagc cggcgcacct gcacgtgctg gatacaaaca
     2641 tctgctccac gcgctgtgct gaagagtatg gcaatccgtg tcagaggttc tgtccggcgg
     2701 cggtttacga gatggttgac gacggcggca atggcaggcg aaagctgcaa ataaacttct
     2761 caaactgtgt gcactgtaag acctgcgaca tcatggatcc gtaccagatc atcaactggg
     2821 taacgccgga aggtggcggg gggcccgatt ataagggcat gtgaaaaagt ctgcgtccaa
     2881 agtccaaagt ctttgtgggt gtgcggacgt tgaacttt
//
LOCUS       DASUEX010000221         4520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1284154, whole genome shotgun sequence.
ACCESSION   DASUEX010000221 DASUEX010000000
VERSION     DASUEX010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4520
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1284154"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..652)
                     /gene="ispH"
                     /locus_tag="VFV61_03735"
     CDS             complement(<1..652)
                     /gene="ispH"
                     /locus_tag="VFV61_03735"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEX4899615.1"
                     /translation="MEVLLADEYGFCFGVERAVEMVEDAVDEGRGPIRSLGPLIHNAQ
                     EMERLGGKGVSTIDTPVEADGSVAVIRAHGVTPLVQLELEQRAAKVIDATCPFVTRVQ
                     HLAERAAEQGRDVVVAGNPDHPEMIGVVGYAPNNTYVVRDADEVAALPTLRSPLVVSQ
                     TTIKLKTFLEVAEAVRAKADAEPQVINTICSATRDRQDAARALAGEVDAFYVIGGRH"
     gene            complement(681..962)
                     /locus_tag="VFV61_03740"
     CDS             complement(681..962)
                     /locus_tag="VFV61_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899616.1"
                     /translation="MDEEKDPINSAYNAICDAARYLGDASYAVLPRDMAHSLGEFKKS
                     FLNNVRYLVDKDIEWVDARVAGGDRLREEWKRACTRDDSTVTGEPEAAS"
     gene            complement(1209..2228)
                     /locus_tag="VFV61_03745"
     CDS             complement(1209..2228)
                     /locus_tag="VFV61_03745"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEX4899617.1"
                     /translation="MSTKMAVTPDNILQLGFAFFGSKTLLSAVELGVFTELAEQPLSA
                     EQLRQRLQLHPRSFLDFFDALVALGMLQRDGDLYANTPETDHFLVRGKLNYIGGFLEM
                     ANERLYRFWGSLTEGLKTGLPQNEVKTGGPGLFETLYADPERLKLFLAAMTGLSMGAS
                     IAIAQKFPWDKYKTVVDVGGAQGGLLVQLCLAHQHLRGTNFDLDVVGPIFDEYVAVNG
                     LSERLKFQPGNFFDQPLPGADVITMGHILHDWDLEQKRMLLDKAYAALPEGGALLVFE
                     ALIDDERKQNAFGLIMSLNMLIETPGGFDYTGKDCTGWMRDAGFRETRVEHLIGPDSM
                     VIGIK"
     gene            complement(2287..>4520)
                     /locus_tag="VFV61_03750"
     CDS             complement(2287..>4520)
                     /locus_tag="VFV61_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899618.1"
                     /translation="GLGMALALTRNMMKTFLEYHPAVLHDLHESVPFLYTSTGTGPYN
                     AWLDPITIDEWQLLAYHEVEEMTKRGVPGVWTHGFYDGWAPNYMFYVANGHNAIGRFY
                     ETFGNGGADTMDRTVTQQAQRDWFRPNPPHARVRWSLRNNVNMQQSGVLLALNFVANN
                     KERFLQNFYIKSKRSVAKATNEGPAAYVVPADQSRLVEAADMVNLLRLMGVEVHTANK
                     EIAVKDQKFPAGSYVIRMDQPYSRMADMLLDTQYYNVNDPRPYDDTGWTLGALRNVKT
                     VRITEKEVLSAPMSMLTTDARVQGRLTGVANAAGYVINHNTDNTLATLRYRLKDVRIG
                     AAEEAFKIGAQQFNSGSFIIKTEGNPGDVRQKLEAAVTELGLTAQAVEKLPEVKTHDL
                     VAARIAILHTWTNTQNEGWYRIEFDRLQIPYSYISDQLVRNTPNLRDKFDVIIFPPLG
                     GSAQQIVNGIPLRGEPIPWKESQLTPNLGHSPDQTDDMRGGMGMAGIVNLQKFVEAGG
                     LFVVIANNSRIPIDYGMTTGVSIQEPRQLQARGSVFTAQFSDRKSPIAYGYDETVPIY
                     FNQAPLFQVATQGGGGGGGGGGAPGAAGGAQRATGRGSLTDGDVIQAMPQAAPAPSPS
                     PARAGEEELTEEQRLQLGPYFTPLAQRPRVVLRFASDEKKLLISGMLAGGAELANAPA
                     VIDVPVGLGHVLMFANNPMWRHQTQGSFFLLFNAAMNFDSLNTERAQPPPRPRTGATS
                     EEQ"
BASE COUNT          983 a         1340 c         1194 g         1003 t
ORIGIN      
        1 aatgccgccc accgatcacg taaaaagcat ccacctctcc agcaagcgcg cgcgctgcgt
       61 cctggcgatc ccgagttgcc gaacaaatcg tattgattac ctgtggctcg gcatccgctt
      121 tggcccgcac cgcttccgca acttccagga acgtctttaa ctttattgtt gtttgggata
      181 ccacgagtgg ggagcgcaag gtcggtagcg ctgctacctc atccgcatcg cgcacgacgt
      241 aagtgttgtt cggagcatag ccgacgacac caatcatctc gggatgatcg ggatttcccg
      301 ccaccacgac gtcgcgtccc tgttcggccg cgcgttcggc gagatgctgc acgcgcgtaa
      361 caaaaggaca ggtcgcatca attactttcg ccgcacgttg ttccagctcg agctgcacca
      421 aaggagtgac accgtgcgcc cgaattaccg cgactgatcc gtcagcctcc acgggtgtat
      481 cgattgtgga aacccccttg ccacccagtc gctccatctc ctgagcattg tgaatcagag
      541 gacccagcga acgaatcggc ccgcgccctt cgtcgacggc gtcttccacc atctccacgg
      601 cacgctcgac cccaaaacag aatccgtatt cgtcagccag taagacttcc atgatttcct
      661 tagcttagtg aaatgagcct ttagcttgcc gcttcaggtt cgcctgtgac tgttgagtcg
      721 tcgcgggtac aagcgcgttt ccattcctca cgtaaacgat cgccaccggc aacgcgcgcg
      781 tctacccact cgatatcttt atcaaccaga taacgcacgt tgttcaaaaa tgacttctta
      841 aactctccca gactgtgtgc catatcgcgt ggcaagacag cgtaggaagc atcgcccaga
      901 tagcgtgcgg cgtcacaaat tgcgttgtaa gccgaattaa ttgggtcttt ctcttcatcc
      961 atgatcgata acctccaaag gacaaaagcc gtcaccacga cggccagcta attctagcac
     1021 caactgcttt acgtccgcac gggctgactc gttccgcaag atacgggaaa ggacggggga
     1081 agagggaaac gggaagaggg ggaggtggaa cagggaaaaa gggaagacaa atcgattctt
     1141 ctctatgccc tgcttccctt ttccccctcc ccatttccct tttccccggt tccccctctt
     1201 gattcagttc acttgatacc gattaccatc gaatcaggtc cgatcaaatg ctccactcgc
     1261 gtttcgcgaa aaccagcatc acgcatccag ccggtgcagt cctttccggt atagtcgaat
     1321 ccgcccggtg tttcgatcag catgttcagg ctcatgatca agccaaacgc gttctgcttc
     1381 cgctcgtcat caatcaacgc ttcaaacacc aggagagccc cgccttcagg cagggccgcg
     1441 taggctttat cgagcagcat gcgtttctgc tccagatccc agtcgtgcag gatgtgtccc
     1501 atcgtgatca catctgcgcc aggcaacggc tggtcaaaga agttgccggg ctggaacttt
     1561 agtcgttcac tcaatccgtt aaccgcgaca tactcgtcga aaatcggacc gacgacatcg
     1621 aggtcgaagt tggttccacg caagtgctgg tgtgccagac agagttgcac cagcagacct
     1681 ccttgcgcac cgccaacatc cacgactgtt ttgtacttgt cccaggggaa cttctgcgca
     1741 atggcgatgg acgcgcccat gctaagcccc gtcatggccg cgaggaagag tttcaggcgt
     1801 tcggggtcag cgtaaagagt ctcgaaaagg cccgggccgc cggtcttgac ctcgttttga
     1861 ggtaatccgg ttttcaaacc ttcagttaac gatccccaaa aacgatagag ccgctcattt
     1921 gccatttcga ggaagccgcc gatgtagttc aacttgccgc gcaccagaaa gtgatccgtt
     1981 tccggcgtgt tggcatagag atcgccgtcg cgttgtagca tgccaagggc aacgagggcg
     2041 tcgaagaaat cgagaaagct gcgcgggtgc agttgcaacc tctggcgcaa ctgttctgcg
     2101 cttaagggct gctccgccag ctctgtaaac acgccgagtt caaccgcgct aaggagagtt
     2161 ttagatccga aaaacgcaaa ccccagttgc aaaatgttgt caggcgtgac cgccatcttt
     2221 gtcgacatga aattgatcct ccaaattaac catgcttgag tatcgagact cggctttgcc
     2281 gaggggtcac tgctcctcgc tcgtcgctcc cgttcgcggg cgcggcggtg gttgggctcg
     2341 ctccgtgtta agcgaatcga aattcatcgc cgcattgaag agcaaaaaga aacttccttg
     2401 cgtttgatga cgccacatcg gattgttagc aaacatcaga acatggccga gtccgaccgg
     2461 gacatcaatc accgccggtg cgttcgccaa ctccgcacca ccagccagca tgccgctgat
     2521 caaaagcttc ttctcatcgg aagcaaagcg cagcaccact cgcggccgtt gtgcaagcgg
     2581 agtgaagtag ggcccgagtt gcaaacgctg ctcctcggtc aactcttcct cacccgctct
     2641 tgctggtgag ggcgagggcg ccggtgctgc ttgcggcatg gcctgaatca cgtcgccatc
     2701 cgttaagctc cctcgtcccg tcgctcgctg tgcgccgcct gcggctccgg gcgcgccgcc
     2761 gccaccgcca ccgccgccgc ctccctgcgt cgccacctga aacaagggcg cctgattaaa
     2821 ataaatcggt acggtttcgt cgtagccata cgcgatcggg cttttacgat cgctaaactg
     2881 cgctgtgaag accgaacccc ttgcctgtaa ttggcggggc tcctgaatcg agactccggt
     2941 cgtcatccca tagtcgatcg gaatacgcga gttgttggca atgaccacaa acagaccgcc
     3001 cgcttcgaca aacttttgca ggttcacgat accggccata cccatgccgc cgcgcatgtc
     3061 atcggtttga tcgggcgaat ggccgagatt cggggtcaac tgtgattctt tccagggaat
     3121 cggttcaccg cgcaggggaa tgccgttgac gatttgctga gccgaaccgc cgagcggggg
     3181 aaagatgatg acatcaaact tgtcgcgcag atttggtgtg ttgcgcacca actgatcgga
     3241 aatgtacgaa tagggaatct gcaaccgatc aaactcaatg cgataccagc cttcgttctg
     3301 cgtgttagtc caggtgtgaa gaatggcgat gcgcgccgcg acaaggtcgt gagtcttgac
     3361 ctccggaagc ttctcgacag cctgggcggt caaccctaac tcggtgacgg cggcttccag
     3421 cttctgtcgc acgtcgcccg gatttccttc ggtcttgatg atgaacgaac cggagttgaa
     3481 ctgttgcgcg ccgatcttga atgcttcttc ggcggcaccg atacgcacgt ccttgagacg
     3541 atagcgcagc gtcgccagcg tgttgtcggt gttgtgattg atcacgtacc cggcagcatt
     3601 cgctactccg gtcaatcttc cctgcacgcg agcatcggtc gtgagcatgc tcatcggtgc
     3661 gctgagcact tccttctcgg taatacgaac tgtttttacg ttgcgcaaag cgcccaacgt
     3721 ccaacccgta tcatcgtaag gacgcggatc gttcacgttg taatactgcg tatccaaaag
     3781 catatcggcc atccgcgagt aaggttgatc catgcggatc acgtacgatc cggctggaaa
     3841 tttttgatcc ttgacggcga tttctttgtt tgccgtgtgc acctcaacgc ccatcagcct
     3901 cagcaggttg accatgtcag ccgcttccac cagccgtgac tgatcagcgg gaacaacgta
     3961 agcagccggg ccttcattgg tggctttggc aacggaacgt ttgctcttga tatagaagtt
     4021 ctgcaggaat cgctctttat tgttggcgac aaagttcagg gccaacaaca cacctgactg
     4081 ctgcatgttg acgttattgc gcagcgacca ccgcacacgc gcgtgcggag ggttagggcg
     4141 aaaccaatcg cgctgcgcct gttgcgtcac tgtccgatcc atcgtatcag cgccgccatt
     4201 gccgaaagtt tcataaaagc ggccgatcgc gttgtggccg ttggcaacat agaacatata
     4261 gttcggcgcc cagccgtcat agaagccatg tgtccaaacg cctggcacgc cgcgcttagt
     4321 catctcctcg acttcgtgat acgcgagcag ttgccactca tcaatcgtga tgggatcgag
     4381 ccaggcgtta taaggtccgg taccggttga ggtgtaaaga aaaggaaccg attcgtgcaa
     4441 gtcgtgcagc acggccggat gatattccag aaacgttttc atcatgttgc gcgtcagcgc
     4501 gagggccata ccgagaccat
//
LOCUS       DASUEX010000222         4344 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_260149, whole genome shotgun sequence.
ACCESSION   DASUEX010000222 DASUEX010000000
VERSION     DASUEX010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4344
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260149"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            298..1479
                     /locus_tag="VFV61_03755"
     CDS             298..1479
                     /locus_tag="VFV61_03755"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX4899619.1"
                     /translation="MSQSQTAIGSSFGSPLTTLSEDEQMFRGSVREFAEGELRPRVEA
                     MDEQAKLDPALIKQCFDLGLMGIETPEEFGGAGASFFTAILAVEELSRVDASVGVFVD
                     VQNTLVNNALIHWGNQEQKKKYLTRLAAGAVGAYALSEAGSGSDAFAMQTRAVDKGDH
                     YLINGRKLWITNGNEAEIFILFANANPEAGYRGITAYIVEKSFPGFSVGKKENKLGIR
                     ASSTTELILEDCAVPRENVLGEVGKGYKTSIETLNEGRIGIGAQMIGIARGAMDAALA
                     YTGERQQFGKSINQFQGVQFQLAEMATELEAARLLVYNAARMKDAGLNFVKEAAIAKL
                     YSSRAAERISSVAIELYGGYGFVKDYPVEKYWRDSKIGAIYEGTSNMQLQTIAKLMIG
                     K"
     gene            1660..1986
                     /locus_tag="VFV61_03760"
     CDS             1660..1986
                     /locus_tag="VFV61_03760"
                     /inference="COORDINATES: protein motif:HMM:NF024574.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899620.1"
                     /translation="MSRIDIFKQILENDPGNSNVLFGLAKEYEKAGDDRQLIGILDRY
                     LSSADDEGNAYGMLASAHERLGQRDQARQAYERGIAAATAHGHPSMAEEYRTILTTEF
                     PDNSSE"
     gene            1992..4283
                     /locus_tag="VFV61_03765"
     CDS             1992..4283
                     /locus_tag="VFV61_03765"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase E1 component subunit alpha/beta"
                     /protein_id="HEX4899621.1"
                     /translation="MSDTKVLKLVRKSGAETEHPDPPEEKEATERARPAVQLSVAQAA
                     TAPAAAPSAAGKPRAAKTAKPRERGQLDREQMLAIYRTMYLSRKLDDKEIQLKGQNKI
                     FFQISGAGHEAVLVAAGLVLKPGYDWFYPYYRDRALCLQLGMTPLEQLLSAVGAEADP
                     NSHGRQMPSHWGHKKLNIVSQSSPTGTQMLQAAGCAEAGYRARLIKELKQKVTGFHAD
                     EVVYVSLGDGTTSEGETWEALNTICNLKLPVLILLEDNGYAISVPVEVQTCGADASKL
                     VENFPNLLVQRVDGTDVLASLEAMQKAVDFIRAGNGAALVHAKVIRPYSHSLSDDEKL
                     YRPDEERVSDSERDPIKRFGSHLVQEGVIDQDGLQQIKDDIDREVTAAADEALTSPQP
                     DPQSATRYVYSPDVDPAGKDFDNEAGAELTGNPGTMVDLINRCLHTEMARDPRILVFG
                     EDVADVSRDVYLEKVKGKGGVFKVTANLQRKFGSDRVYNSPLAEANIVGRAIGLATRG
                     LKPVVEIQFFDYIWPAFHQIRNELALMRWRSGGDWKAPLVIRVPIGGYLKGGAVYHSQ
                     SGVSTFTQIPGLRVVYPSNALDANGLLRTAIRCDDPVLFLEHKHLYRQAYNKSQYPPD
                     DFMIPFGKARTVREGTDLSIITYGALVQRSVVAAKQAEQQGVSVESIDLRTLAPYDWD
                     AIVATVKKTSRVIVAHEDCLSFGYGAEIAARIGDELFEYLDAPVKRVAAIDTFVAYAP
                     QLEDAILPQATDVAQAISELKAY"
BASE COUNT         1035 a         1100 c         1252 g          957 t
ORIGIN      
        1 gtgtacgcaa aaaggagcaa gccgagggcg gcgaagataa tccccagagg agcaaacttc
       61 tttgatttac ttcggctctt cgtcgattca ggcatcgtta ttcttgggag acgcgagtta
      121 gctcgcaaca gtttaggtgc ggattgcttg cctatcatgc atccgcgggt ggagcggggc
      181 aagcttgctg cctacggcca tcaccgtgca gttttccgtt tggacattgt ccgtagcgtt
      241 tgattatgat gctaaccgtt tcccgaacaa atttgttgcg gagttaaggt tgaatagatg
      301 agtcaaagcc aaactgcgat tggatcaagt ttcggatcac cgctaacaac gctctccgaa
      361 gatgagcaaa tgtttcgcgg cagtgtgcgg gagtttgccg aaggcgaatt gcgcccgcgt
      421 gtggaagcga tggacgaaca ggcgaagctc gatccggcgc tgatcaagca gtgttttgac
      481 ctgggcttga tgggcattga gacgcccgag gaatttggcg gcgccggtgc ctcatttttc
      541 acggccatac ttgcggttga ggagctttcg cgcgtggatg cgtcggtagg agtatttgtc
      601 gacgtgcaga acacgcttgt caataacgcg ctcattcatt ggggcaatca ggaacagaag
      661 aagaaatacc tgaccaggct cgcagccggt gcggtcggag cctacgcctt gagtgaagcc
      721 ggttccggtt cagatgcctt tgccatgcag acacgagctg tcgataaagg cgaccattat
      781 ttgatcaacg gccggaagct gtggatcacc aacggcaacg aagcagaaat ctttattctg
      841 tttgcaaatg ccaatcctga agctgggtac cggggtatca cagcctacat cgtggaaaag
      901 agttttccgg gattctcagt tggaaaaaag gaaaacaaac tggggattcg cgcgtcttcg
      961 acgacggagt tgattctgga agattgcgct gtgcccaggg aaaatgtttt gggcgaagtc
     1021 ggaaaaggtt acaagacgtc aatcgagact ttgaatgaag gccgcatcgg cattggcgcg
     1081 cagatgattg gtatcgcgcg cggtgccatg gacgcggccc tcgcttacac cggcgagcgc
     1141 cagcagtttg gcaagtcgat caatcagttt cagggcgtgc agttccaatt ggcagagatg
     1201 gcaaccgagc tcgaagccgc acgcttgctc gtctataacg cggcgcggat gaaagacgca
     1261 ggcttgaact tcgtcaaaga agcggcaatt gccaaactat attcctcgcg cgccgccgag
     1321 cgaatcagct cggtagcgat cgagctctac ggtggttatg gtttcgttaa agactatccg
     1381 gtggagaaat actggcgcga ctcaaagatt ggcgcaattt acgaaggcac ttccaatatg
     1441 cagttgcaga cgattgcaaa gctcatgatt ggcaagtgat cagttttgcc acgggactgg
     1501 tttctgttct ttgaactttg tacttagtac tttgttgtgg ggtgaaaccg acgcacttcg
     1561 ttttgtagaa gctttcaagt gggcattgtc agaacaaaga actaagtaca aagcacaaag
     1621 ttctaaaacc taaggcgtca aaacgagttt aggaccgcga tgagtcgcat agacattttc
     1681 aaacagattc tcgagaacga tcccggcaac agcaatgtgc tgttcggcct ggcaaaggaa
     1741 tatgaaaagg cgggcgacga tcgtcagttg atcgggattc ttgatcgata cctttccagc
     1801 gcggatgatg aaggcaacgc gtacggcatg ctggcgtcag cccatgagcg actcggccag
     1861 cgcgatcagg cgcgacaggc ttatgagcgc ggcattgcgg cagcaacggc gcatggtcat
     1921 ccttcaatgg ctgaagagta tcgcaccatc ctgaccacgg agtttcccga caactcttcc
     1981 gagtaaacat tatgagcgat acaaaagttc tcaaactggt gcgcaagagc ggggctgaaa
     2041 ctgagcaccc ggacccacct gaagaaaagg aagcaacaga gcgagcgcgt ccggcggtac
     2101 aactctccgt tgcccaggca gcgacagctc cagcagcagc gccatcggcc gcggggaaac
     2161 cacgcgccgc gaaaactgcg aaaccccgcg agcgcgggca actggatcgc gaacagatgc
     2221 tggccatcta tcgcacgatg tatttgtcgc gaaagctcga cgacaaagag atccaactta
     2281 aaggccaaaa caagatcttc tttcaaatta gtggcgccgg acatgaagcc gtgctcgtcg
     2341 ctgccggttt ggtattgaag ccgggttacg attggtttta tccctactac cgcgaccgcg
     2401 cactttgcct gcaactgggt atgacgcctt tggagcagtt gctttccgca gtgggggccg
     2461 aagcggatcc gaattcccac ggccgccaaa tgccatcgca ctggggccac aaaaaactca
     2521 acatcgtttc ccaatcgtct cccacaggta cgcaaatgct acaggctgcg ggctgtgcgg
     2581 aagccggcta tcgcgcgcgg ttgatcaagg aactaaaaca gaaagtcacc gggtttcatg
     2641 ctgacgaggt ggtttatgtc tcgctcggcg acggcaccac cagtgaaggg gaaacgtggg
     2701 aagcactgaa caccatctgt aacctcaaac tgccggtatt gattctcctg gaagacaatg
     2761 gttacgccat cagcgtaccg gttgaggtgc aaacctgcgg ggccgatgcg tcgaagctgg
     2821 ttgagaattt tccgaatctt ttggtgcagc gagttgatgg cactgatgta ctcgcttcat
     2881 tggaagcgat gcagaaggca gtcgacttta ttcgcgcggg taatggtgcg gctctggttc
     2941 atgcaaaggt aatccgtccc tattcccatt cgctttcgga cgacgaaaaa ctttatcgcc
     3001 ccgatgaaga acgggtttct gattccgaac gcgatccaat caagcgtttc ggttctcacc
     3061 tcgttcagga aggcgtcatc gatcaggatg gcttgcagca aataaaggac gatatcgata
     3121 gggaggtcac tgctgccgcc gatgaggctt taaccagtcc gcaacccgat ccgcaatcag
     3181 cgactcgtta cgtttactca ccagacgttg atcccgcggg gaaagatttc gacaacgaag
     3241 ccggcgccga actgactggc aatccgggta cgatggttga tctgatcaat cgctgcctgc
     3301 atacggagat ggcgcgcgat ccgcgcatcc tagtctttgg cgaagacgtc gcagatgtct
     3361 cacgggatgt ttatctggaa aaggttaagg gcaagggcgg cgttttcaag gtaacggcga
     3421 acctgcaacg gaagttcgga agcgatcgcg tttacaactc gcccctcgcc gaagcaaata
     3481 ttgtcggccg ggcaatcggt ctggccacgc gcggtctaaa gccggtggtt gagattcaat
     3541 tcttcgacta tatctggccg gcgtttcatc agattcgcaa tgagctggcg ttaatgcgct
     3601 ggcgctcggg aggcgattgg aaagcgccgc ttgtaattcg ggttccgatt ggcggttacc
     3661 taaaaggcgg cgcggtttac cactcgcaat cgggagtttc aacgtttacg cagattcccg
     3721 gattgcgggt cgtctatccg tcaaatgcgc tcgatgcgaa tggcctgctg cgcaccgcga
     3781 ttcgttgtga cgatccggtg ttgttcctcg agcacaaaca tctctatcgc caggcttaca
     3841 ataagtcgca atacccgccc gacgacttca tgattccttt tggcaaagcc agaacagtgc
     3901 gtgaagggac agatctttcg atcatcactt acggcgccct cgtgcagcgt tcagtagtgg
     3961 ctgcgaaaca ggctgaacaa cagggagtca gtgtcgagag tatcgacctg cgcacgctcg
     4021 cgccctacga ttgggacgcg attgtggcta cggtcaagaa aacgagccgc gtgatcgtgg
     4081 cccatgaaga ctgtctgtcg tttggttatg gcgccgaaat cgccgcgcgg attggggacg
     4141 agctgttcga gtaccttgat gctccggtga agcgcgtcgc cgcaatagac acgtttgtcg
     4201 catacgcgcc ccaattggaa gatgcgattc tgccgcaggc cacggatgtg gcacaggcga
     4261 tcagcgagtt gaaagcttac taagtgtcga tttcccgaaa gtccaaagtc caaagtccaa
     4321 tgtccaacgt ccgctgccct ggcg
//
LOCUS       DASUEX010000223         2800 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1076472, whole genome shotgun sequence.
ACCESSION   DASUEX010000223 DASUEX010000000
VERSION     DASUEX010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2800
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1076472"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="VFV61_03770"
     CDS             <1..272
                     /locus_tag="VFV61_03770"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX4899622.1"
                     /translation="TQQAPIIVTQPATGGSTSGPDDYTIQSEVDKRILDDPNLSTLGV
                     TVSVISGKATLMGTVKSEAIKTQFERLVRSVRGVKDIDNQITVSG"
     gene            complement(355..786)
                     /locus_tag="VFV61_03775"
     CDS             complement(355..786)
                     /locus_tag="VFV61_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899623.1"
                     /translation="MRDEWTEAATTTHQDHVIAHVIGTTVLGYVKLEEAVHLLLDIGF
                     IWKIYVDGEMGLLPHPVAVAELEVEDEAQAQIKLDVDLLLRGGDESALARFLPIRVTS
                     PITSVQVFVRGTEHKLLLEGETGAIQIQTSVSDDLIQVREG"
     gene            898..1539
                     /locus_tag="VFV61_03780"
     CDS             898..1539
                     /locus_tag="VFV61_03780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEX4899624.1"
                     /translation="MDQDVIRFGLAAFVTLLVVVDPPGVVPIFVALTKDDTHARRRAI
                     LTRAVLIAFGVALFFLIAGRSVLSYLGVSVHAFAISGGILLFATALPMLFGQRGGLQA
                     PEEKERGIVGEDISVFPLAIPMLSGPGVIATTLLLTSQAAGDWRRLGALATALALVFF
                     VAFISLYLGARLIARLGEGGVHIATRVMGIVLAALAVQYVLNGITGYYSALLR"
     gene            complement(1518..2426)
                     /locus_tag="VFV61_03785"
     CDS             complement(1518..2426)
                     /locus_tag="VFV61_03785"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEX4899625.1"
                     /translation="MLTASAVHSPETSKGLAGLNWTHTFLVVSLHAFGLLGLWFWPRT
                     VDLVLFLSLYFITTFSIGIGYHRLLTHRGFVSPRWLRRILAWGGAAALQGGPARWAAI
                     HRRHHQMADKHGDPHSPLMGFLHAHVGWIMKRDAAEGSDYRELVPDVSANDPWLRILD
                     YKFLFTLPWVLTVLLCYGIGGWRGVLWGTVVRTLTLWHFTWCINSVCHVIGKRPNQTR
                     DESRNVWWLGLLTLGEGWHNNHHAHPRAAIHGWRWYQVDISGYVIRLLAKVGVIGKVL
                     REPSLQLSPARITARSSSESQRKSAL"
     gene            complement(2484..2720)
                     /locus_tag="VFV61_03790"
     CDS             complement(2484..2720)
                     /locus_tag="VFV61_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899626.1"
                     /translation="MNGLRVYTKPNNTNGDQKVFYSRRGNGPYYRWFYEEKANEWRAS
                     RVITADFTSHSLSMATWKAVPVALQTRLGEHYLD"
BASE COUNT          636 a          784 c          736 g          644 t
ORIGIN      
        1 ctacgcagca ggcgcccatt attgtgacgc agcccgcaac aggcggatcc accagtggcc
       61 cggacgatta cacgattcaa tcagaagtcg acaaacgcat ccttgacgat ccgaatctct
      121 cgactttggg agtgacggtt agcgttatta gcggcaaagc gaccctgatg ggcaccgtta
      181 agagcgaagc gatcaagacc cagtttgagc gactcgttcg cagcgtcaga ggcgtcaagg
      241 acatcgacaa tcaaattact gttagtggtt gagtttagca gcgccagttg ttggcgtctc
      301 ttgaaacttg ccgattgatt cagggcatgc cgcgggcgtg cccttttttt gtgttcagcc
      361 ctcacggact tgaatcaagt cgtcactgac tgaggtttgg atctgaatag cgccggtttc
      421 accttcaagc aacagcttgt gttcagtgcc acgtacaaag acctgcacac ttgttatcgg
      481 ggatgtgact ctgattggca agaagcgcgc taaagctgac tcatcaccac cgcgcagaag
      541 caaatcgaca tcaagcttga tctgcgcctg cgcctcgtcc tcaacctcaa gttcggcaac
      601 cgcaaccgga tgcggcaata agcccatctc gccatcgaca tagatcttcc agataaatcc
      661 gatgtctagt aacagatgga cggcctcctc aagctttacg tatccaagca ctgtagtgcc
      721 gattacatga gcaatgacat gatcctgatg agtcgtcgtc gctgcctctg tccattcgtc
      781 tctcattgaa atcccctctc ccgaaaccaa cgaccaacgt caggtttagc aatcggaatt
      841 agccaagcgg caattggcaa tcaccaacgt tcccgtctat tctcaaggca accaatcatg
      901 gatcaggacg taatcagatt tggtctggcg gcgtttgtca cgttgctggt ggtcgtcgac
      961 ccgccgggcg tggtcccgat cttcgtcgca ctgaccaaag acgacactca tgcccgccgc
     1021 cgcgcgattc taacaagggc agtgctgatt gcctttggcg tcgctctctt ctttcttata
     1081 gccgggcgct cagtgctttc ctatcttggc gtgtcggttc atgccttcgc aatcagcgga
     1141 ggcatcttgc ttttcgctac ggcgctgccc atgttgtttg gacaacgcgg cggcttgcag
     1201 gcacccgagg aaaaagaacg cggcatagta ggcgaagaca tttctgtgtt tccgctggcc
     1261 attcctatgc tctccgggcc gggagtgatc gccacaactt tgctgctgac ttcacaggca
     1321 gcaggcgatt ggcgccgcct gggagcgctt gccacagcgc tcgcgctcgt ctttttcgtt
     1381 gcttttattt cgctttacct tggtgcgagg ctaatcgccc gcctgggtga gggcggtgtg
     1441 catattgcca caagagttat gggaatcgtg ctggccgcgc ttgccgtgca gtatgtgctt
     1501 aacggcatca cgggttacta cagtgcgctc ttgcgctgag actctgatga tgaacgggcg
     1561 gttatccttg ccgggcttaa ctgcaggctc ggctcgcgca atactttacc aatcacgccg
     1621 actttggcta gcagacggat tacgtatccg gaaatatcga cctggtacca acgccagcca
     1681 tgaatcgctg cccgcggatg cgcgtgatga ttgttatgcc agccttcgcc cagtgttaat
     1741 aaccccagcc accaaacatt tctgctttcg tcgcgcgtct gattgggccg cttaccaatc
     1801 acgtgacaga cgctgttgat gcaccaggtg aaatgccaca aagttagggt gcgcaccacc
     1861 gtgccccaaa gaacaccgcg ccaacctcca atgccgtaac aaagcaaaac cgttaaaacc
     1921 cagggcagag tgaaaaggaa tttatagtct agtattcgca gccagggatc gttagccgat
     1981 acgtcaggaa ccagctcgcg gtagtcactg ccctccgccg catctcgctt cataatccaa
     2041 ccaacatgcg cgtgcaaaaa ccccatcagc ggtgaatgcg gatcgccgtg cttgtcagcc
     2101 atttgatgat gacgccggtg aattgccgcc cagcgtgccg ggccaccctg cagtgctgcc
     2161 gcgccacccc aggcaagtat tcgccttagc caacgcggcg aaacaaatcc ccgatgcgtg
     2221 agtaaacgat ggtagccgat gccgatcgaa aaagttgtaa tgaaatagag actgaggaac
     2281 aggacgaggt cgactgtccg cggccagaac caaagcccga gcaggccaaa cgcgtgaagt
     2341 gaaacaacaa gaaaggtgtg ggtccagtta agaccagcaa gtccctttga agtctcaggt
     2401 gagtgaactg ccgaagcggt aagcaaaagt atccttaaat ccggaggaac tcaggctttc
     2461 tccggaaagt gatgcggccg atgctagtcg aggtagtgtt cgcctaatct ggtttgcagt
     2521 gctaccggga cagccttcca ggttgccatc gaaaggctat gagaagtgaa atcagccgtg
     2581 atcacgcgtg aggcgcgcca ttcattcgct ttctcttcgt agaaccaacg atagtaggga
     2641 ccattcccgc gacgactata gaagaccttc tggtcgccat tcgtgttatt cggttttgtg
     2701 tagactctta atccgttcat gaccactcag cttggctcgc taaatcgcac taaacctcgc
     2761 tttatttcct cgagggcgat gctggtgcgt ttgcggcggt
//
LOCUS       DASUEX010000224         4021 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_962401, whole genome shotgun sequence.
ACCESSION   DASUEX010000224 DASUEX010000000
VERSION     DASUEX010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4021
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_962401"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1050
                     /locus_tag="VFV61_03795"
     CDS             <1..1050
                     /locus_tag="VFV61_03795"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX4899627.1"
                     /translation="VEHVRKERAREFNFRQIIGASPAMKKMLGLAAKVAESDVSSVLL
                     QGESGTGKDLVAKAIHYGSHRADHPFIAINCAALPATLIESELFGYEKGAFTDAKSRK
                     EGLFEQAEGGTLLLDEIGELELALQAKLLRVLEEGAFRRVGGLKDIALDVRVLAASNR
                     DLKTESEAGRFRLDLYYRLSIIQIDIPPLRDRGDDVLLLAQHYIDTIGSRLRLRKKIK
                     GLSPAVVEVFRGYEWPGNVRELRNVIERALILEDNDTITLEYLPGSLLGLTGQDGGPT
                     TPSGSRSAQFVLPNEGISLDEAELSFVQQAIDRSGGNQTRAAELLGISRDQLRYRLKK
                     LDEIRGLATGSESSS"
     gene            1063..2991
                     /locus_tag="VFV61_03800"
     CDS             1063..2991
                     /locus_tag="VFV61_03800"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX4899628.1"
                     /translation="MSNTSPPETQLQQGEALERSRKELADIRFALDEAAIVAITDQRG
                     IISYANDKFCEISGYSRAELLGQDHRIINSGYHPKEFIRDLWTTIAGGKVWHGEIKNR
                     AKSGRFYWVDTTIVPFLNAEGKPYQYVAIRSDITERKLGEERIREQAALLDQAQDAIL
                     VRDLNQNILFWNKGAERIYGWSAPEALGKNVFDLLLKERSPQFDTARRAVLEAGEWSG
                     EIQQYRRDGSEIIVESRWTLVRDDQGAPTSILVINTDITDRKRMEAQFLRAQRMESIG
                     TLAGGIAHDLNNVLSPILMAIDMLQLKATDEASKKWLEVLRTNAERGGNMVRQVLSFA
                     RGVEGERVALQPKHLIKEIVKILRETFPKSVEINFELPDDLWIISADATQMHQVLMNL
                     CVNARDAMPEGGSIFIRAENTVVDENYARMHLEAKPGRFVLITVSDTGPGIAPEIQSR
                     IFEPFFTTKEMTKGTGLGLSTALTIVKSHGGFINVYSELHKGTQFSLYLPALDSPETV
                     KAGALQTDLPLGNGELILVVDDEESIREITRSTLETFGYRVLTANDGTEAIALYADRK
                     NEVSVVLTDMMMPFMDGPATIRALQKMNRAVKIIAASGLAAGHKAGEASLEGVEMFLG
                     KPYTAEKLLVALAKILKT"
     gene            complement(3151..3687)
                     /locus_tag="VFV61_03805"
     CDS             complement(3151..3687)
                     /locus_tag="VFV61_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899629.1"
                     /translation="MRTTRIMVFALAALITVLLANTAQAQKGVFVSFKGIEIHTGHEE
                     AGEAYGWMCYAKTTGALPGNFTLTMDFAGVKGPGTSTDVTGGAWTLPVYTSTKVSRLR
                     PILEDPYQGVVFGSVEGGGISWDKDGASATVELKLLIRGGTQTMSEMRGSAYLYGTVV
                     YDDKGRGTFEGTIYFDLL"
BASE COUNT          963 a         1048 c         1128 g          882 t
ORIGIN      
        1 gtagagcacg tgagaaagga acgagctcga gagtttaact tccggcaaat cattggcgcg
       61 tctccggcta tgaagaagat gctcgggctg gccgctaaag tggcagaaag cgacgtgtcc
      121 tcggtcctcc tgcaaggaga atcagggacg ggcaaagact tagtggcaaa agcaattcat
      181 tacggatcac accgcgccga ccatcccttc atcgcgatca actgtgccgc gctgccggca
      241 acgctaatcg aatctgagct gtttggttac gaaaaggggg ctttcactga tgctaagtcg
      301 cgcaaagaag gcctcttcga gcaagccgaa ggcggcactc tgctgctcga tgaaatcgga
      361 gaactggaac tcgcactgca agcaaaacta ttgcgcgtct tggaagaggg agccttcagg
      421 cgggtcggcg gattgaagga catagcgctt gatgtgcggg tcctcgctgc ttccaatcgc
      481 gaccttaaga ccgaaagcga ggcgggtcgt ttccgactcg atctgtatta ccgactctcg
      541 attattcaaa tagatattcc accgctgcgc gatcgaggcg acgatgttct gctgctggcg
      601 caacactaca tcgatacgat tggctccagg cttcgcttgc gaaaaaagat caagggatta
      661 tcgcctgcag tagtcgaagt ctttcgcgga tatgagtggc ccggcaatgt gcgtgagttg
      721 cgcaacgtga tcgaacgagc actcattctc gaagacaacg atacgatcac cctggaatac
      781 ctaccgggca gtctgttggg tctgacaggc caggacggcg ggccaacgac gccgagtggt
      841 tcccgctccg cccagttcgt gctacccaac gagggcatct ctctggacga agcggaactt
      901 tcgtttgtgc agcaggcgat cgaccggagc ggcggcaacc aaacacgcgc tgccgaattg
      961 ctcggcattt cccgagatca attgcggtat cgactcaaga agcttgatga aatccgtggc
     1021 cttgcgactg gttcggaaag tagctcctaa gatctgatgg tattgagtaa cacatcacca
     1081 cctgagacgc agcttcaaca aggtgaagcg ctggaacgtt cgcgcaagga acttgcagac
     1141 atcaggtttg cgcttgatga agcggccatc gttgctatta ccgatcagcg cggcatcatc
     1201 agctatgcca acgataagtt ttgcgaaatc tccgggtatt cacgagcaga gctgctcggt
     1261 caggatcatc gcatcataaa ctctgggtat catccgaaag agttcatcag agatttgtgg
     1321 accacaatcg ccggcggcaa ggtgtggcat ggtgagatta agaaccgcgc gaaaagtggc
     1381 aggttctact gggtcgacac gaccatagtc ccctttctca acgctgaagg gaaaccatat
     1441 cagtacgtcg cgatccgtag tgacattacg gaacgcaaac ttggcgaaga gcgcatccgc
     1501 gagcaagccg ccttgttgga tcaagcccag gacgcaattc tggtccgcga tctcaatcaa
     1561 aatattctct tttggaacaa gggtgccgag cgcatttacg gctggtcggc gccggaagcc
     1621 ctcggcaaga atgtgtttga tttgcttctt aaggaacgct cgccccagtt tgacacagct
     1681 cggcgagcag tgcttgaagc tggtgagtgg agtggtgaaa ttcagcagta ccggcgtgac
     1741 ggatcggaaa tcattgttga gagtcgctgg acgctggtgc gtgatgacca gggagcgcca
     1801 acctcgatcc tcgtgatcaa taccgacatc accgatcgca agcgaatgga ggcgcagttc
     1861 ctgcgcgccc aaaggatgga gtccattggc acgctcgccg gcggcattgc tcacgatcta
     1921 aacaacgtac tgtcgccaat cctgatggcg atcgacatgc tgcagttgaa ggcgacagac
     1981 gaggcgagca agaagtggct ggaagtgctg cgcacgaatg ccgaacgtgg cggaaacatg
     2041 gtgcgccagg ttttgtcgtt cgccaggggt gtggaaggcg aacgcgtagc cttgcagcca
     2101 aaacacctga tcaaggagat cgtcaagatt ttgcgcgaga cgtttcctaa gtcagttgag
     2161 atcaatttcg agttgcctga cgacttatgg attatttcgg cggatgccac tcagatgcat
     2221 caggtcttaa tgaacctttg cgtcaacgcc cgcgatgcca tgcctgaagg aggttctatt
     2281 ttcatcaggg ctgagaatac ggtggtagac gagaattacg cccgcatgca cctcgaggcc
     2341 aagcccggcc gttttgttct gataacggtt tccgatacgg gaccgggaat cgcgccggaa
     2401 atacaaagcc gaatcttcga accattcttc accacaaaag agatgaccaa aggtaccgga
     2461 ctgggtcttt cgactgcgct aacaattgtg aagagtcatg gtgggttcat aaatgtctat
     2521 agcgaacttc ataaaggaac acagttttcg ctttacctgc ccgccctcga ttcaccagag
     2581 acggtcaagg caggcgcgtt acagaccgat ctcccgctcg gtaatggcga attgattttg
     2641 gtagtggatg atgaggaatc aattcgcgaa ataacgcgta gcacgctgga gacgtttgga
     2701 taccgggtgc tcacggccaa cgacggtacc gaagccatcg cactgtatgc cgacagaaag
     2761 aacgaagttt ccgttgtgct caccgacatg atgatgcctt tcatggatgg tccggccacc
     2821 attcgggcgc tgcaaaaaat gaatcgtgcg gtgaagatca tcgccgccag cggactcgcc
     2881 gccgggcata aagcgggcga ggcctcactc gaaggcgtcg aaatgtttct gggcaagccc
     2941 tacacggcag agaaactgct cgtggctttg gcaaagattc tgaaaactta gcggggaaat
     3001 tttcggcggg caagtggaag aaaaggcccg gggattgccg attggcgatt gctaattcgc
     3061 ccgcagcgga atgaagtcag ttagtagtgg tcactgacct cgactaatcg gcaatcggca
     3121 atcgccaatc ggcaattgct tgcggttggt tcacagaaga tcaaagtaga tggtgccttc
     3181 gaacgttccg cgtcctttat catcatagac tacggtgccg taaaggtagg cactgccgcg
     3241 catctcgctc atcgtctggg tgccgccccg gataagcagc ttcagttcaa ccgtggctga
     3301 cgctccgtct ttgtcccacg atattccgcc tccctcaacg ctgccgaaaa ccacgccctg
     3361 gtaagggtct tctaagatcg gtctcagccg ggagactttt gtcgacgtgt aaactgggag
     3421 ggtccacgcg ccgccggtca cgtctgtgct ggttcctggc cccttcactc ctgcgaagtc
     3481 catcgtcaaa gtgaaattac caggcaaggc cccggtggtc ttcgcataac acatccaacc
     3541 gtaggcttcc cctgcttctt cgtgaccggt atgtatctcg attcctttga atgatacaaa
     3601 cacccctttt tgtgcctgcg cggtgttggc cagaagaacc gtaatcaagg cagcgagggc
     3661 gaataccatt attcttgttg tcctcatgat tttgctcctt tgagtcatca acactttcgg
     3721 aagccgccgc gactggagcc atcccacccg aataaacgct cggtgctggg tcacatatgc
     3781 gtcttgtgtt tttactacct tgattgcggc aacaaagctc gctggcgttg ctttcgacaa
     3841 ttcaaacatc aacatattgg gcggggagcc gttatgtacg gtgggcaaaa tcgcaactgg
     3901 tatgccgcga ggaacacccg aattatgaat gggggatgga accacccgtg cgcactttaa
     3961 cccagcgctt gagaaggaat tcgcagtttg tcacgacttt gtacgccggc agaatggcaa
     4021 g
//
LOCUS       DASUEX010000225         2665 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1414197, whole genome shotgun sequence.
ACCESSION   DASUEX010000225 DASUEX010000000
VERSION     DASUEX010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1414197"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..1152)
                     /locus_tag="VFV61_03810"
     CDS             complement(46..1152)
                     /locus_tag="VFV61_03810"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX4899630.1"
                     /translation="MINWSDIRQQFPVTERVAYLNTAAAGPLARSVMQAGSEYYLQMM
                     DEGDKRWEEWLEKREAVRRDIAAFINAEPDEIGLTTNTSSGMNVIVDALEGRGEVVSC
                     DLEFPVSTIPWMHRRIPVHLVKNRNGELDLNDVAAAANDKTGVLSFSHVQFSNGLRSD
                     LEAMGNNKGRHAFVVNASQSAGVFAIDVKRMRIDALCATGHKWMLSGYGSGFVYLSRE
                     LQADSKAHAIGWLSVQEPYAMKNNEIVMRYDLSARAELGCPHFAGIFALGAAVELFTG
                     IGIAEIETRALALNRYLTGRLTEAGCRVLSPLRAESFRSAETLVACEDPARVTTQLAE
                     RGVVVTEKPQGIRVATDFFNNEADIERLIAGLNP"
     gene            complement(1176..>2665)
                     /gene="glgB"
                     /locus_tag="VFV61_03815"
     CDS             complement(1176..>2665)
                     /gene="glgB"
                     /locus_tag="VFV61_03815"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF008967.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEX4899631.1"
                     /translation="RQPLSIYEIHFGSWRRFLEEDNRPFKYREMAPILADYVRRMGFT
                     HVEFLPLKEHPYGPSWGYQVGSYYAASARYGTPDDLRYLIDYLHRKEIGVIMDWVPAH
                     FPKDAFALGRFDGSALYEHLDPRRGEHPDWGTYIFNYGRNEVRNFLIANALYWCKEFH
                     IDGLRVDAVASMLYLDYSRNEGEWLPNQLGGRENLEAISLLKELNEVLHRVCPGVMMI
                     AEESTAWPQVSHPVYAGGLGFDFKWNMGWMHDTLKYFQTDPLFRGGNHNALTFGLIYA
                     WSENFILPFSHDEVVHMKGALLNKMPGDEQQKFATLRALYAYMWAHPGKKLLFMGGEF
                     GQWREWNEEVSLDWHLLEKPLHEGVRNLIRDLNRIYKSESALWEADGEAAGFQWIDAD
                     NASENIVSFIRRATTSDRELVCVCNFSPVPKPEHQLGLPRAGKYKLLLNTDSGRYTGL
                     AGKESAKIVKAQAEPLHGQQYSATISLPGMATLWFEVPQAGTKGI"
BASE COUNT          609 a          778 c          682 g          596 t
ORIGIN      
        1 ggtgaagcca gaatatatag gtgcttacga caatctgcgg gagcctcagg gattgagacc
       61 tgcaataagt cgctcaatat ccgcctcatt gttgaagaaa tccgtagcga cacgaattcc
      121 ctgcggcttt tcggtgacaa ccactccgcg ttccgccagt tgagttgtga ccctcgctgg
      181 gtcttcgcaa gcaacaagag tctccgccga gcggaaactc tcagcgcgca agggcgacag
      241 caccctgcaa ccggcctcag ttaggcgacc agtcaaatat ctattcaacg caagggctcg
      301 agtttcgatt tctgcgatgc caatgccggt gaagagttct accgcagcac caagcgcaaa
      361 gattccggcg aagtgcgggc aacccaactc ggcacgcgcg ctcaagtcgt agcgcatcac
      421 tatctcatta ttcttcatcg cgtaaggctc ctgcacgctc agccagccaa ttgcgtgcgc
      481 cttagaatcg gcctgcaact cgcggctcag gtaaacgaac ccggagccgt aaccggacaa
      541 catccatttg tggcccgtcg cgcagagagc atcgatgcgc atgcgcttta cgtcgatggc
      601 gaagactccc gctgactggc tcgcattcac gacgaaagca tgccggcctt tgttattccc
      661 catagcctcc agatcgctgc gcagcccatt ggaaaactgc acgtgactaa aagaaagcac
      721 ccctgtctta tcgttggctg ctgccgcaac gtcattcaga tcgagttcgc cgttgcgatt
      781 ctttacgaga tgaactggaa tcctgcgatg catccacggg atggtcgaaa caggaaactc
      841 gagatcacac gaaacgactt cgcctctgcc ttcgagggca tcgacgatca cattcattcc
      901 tgatgaggtg ttggtggtaa gaccgatctc atcgggttca gcgttaatga aagccgcgat
      961 gtcgcgccgg acagcttccc ttttttccag ccactcctcc cagcgcttgt cgccctcgtc
     1021 catcatctgg agatagtatt cagaacccgc ttgcatcacg gagcgcgcca atggacctgc
     1081 tgcggcagtg ttcagataag ccacccgctc ggtgacagga aattgttggc ggatgtcaga
     1141 ccagttgatc atgataagtt aatccgcgaa gcggactaga ttccttttgt ccccgcctgc
     1201 ggcacttcaa accaaagcgt cgccatcccc ggcagtgaaa tcgtcgccga gtactgttgc
     1261 ccgtgcaacg gttccgcttg cgcttttacg atcttggcgc tctccttgcc ggcaaggccg
     1321 gtatagcgac cgctatcggt attcaagagt agtttgtact tccccgctcg cggcaacccc
     1381 agttgatgtt ctggctttgg caccggggaa aagttacaaa cgcaaactaa ctcgcgatcg
     1441 cttgtcgttg cgcggcgaat aaaggagacg atgttctcgc tcgcgttgtc ggcatcgatc
     1501 cattgaaacc ccgcggcctc gccatccgcc tcccaaagcg cactttcgct tttgtagatt
     1561 cgattgagat cacgaatcag gttccgcaca ccctcatgca gcggtttctc caggagatgc
     1621 caatcgaggc tcacttcctc gttccattcg cgccactgcc caaactctcc gcccatgaac
     1681 aacagcttct ttcccggatg ggcccacatg taggcgtaga gcgcgcgcag cgtcgcaaac
     1741 ttctgttgct catcgcccgg catcttgttc aagagggcgc ctttcatgtg aactacttca
     1801 tcgtgtgaga acggcaggat gaagttttcg ctccaggcat agatcaagcc aaacgtcagc
     1861 gcattatgat tgccgccgcg aaacagagga tcagtttgaa agtacttcag cgtgtcgtgc
     1921 atccagccca tgttccactt gaaatcgaaa ccgagcccgc cggcgtaaac gggatggctt
     1981 acttgcggcc aggcagtcga ttcttcagca atcatcatca cgccgggaca gacgcggtgt
     2041 agcacctcgt tcaactcctt caatagtgag attgcttcca ggttctcgcg gccgcctaac
     2101 tgattcggaa gccactctcc ttcattgcgg ctgtaatcga ggtaaagcat tgaggcgact
     2161 gcatcgacgc gcaggccatc aatatgaaac tccttgcacc agtagagcgc attggcgatc
     2221 aggaaatttc gcacttcatt acgaccgtaa ttgaaaatgt aagtacccca gtctggatgt
     2281 tcgccgcggc ggggatcgag atgttcataa agcgccgacc cgtcgaagcg gccgagggca
     2341 aaagcgtctt tgggaaagtg cgccggcacc caatccataa ttacgccaat ctcttttcga
     2401 tgtaagtagt cgatcagata acgcaggtca tccggtgttc cgtagcgcgc tgaagccgca
     2461 tagtagctgc ctacctggta accccaggaa ggtccgtaag gatgttcctt aagaggcaaa
     2521 aactcgacgt gcgtaaatcc catcctgcga acgtagtccg caaggattgg cgccatctcg
     2581 cgatacttga atggtcggtt gtcctcctcc aggaagcgtc gccacgaacc aaagtggatt
     2641 tcgtaaatcg ataatggttg ccgga
//
LOCUS       DASUEX010000226         3786 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_150997, whole genome shotgun sequence.
ACCESSION   DASUEX010000226 DASUEX010000000
VERSION     DASUEX010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3786
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150997"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1197)
                     /locus_tag="VFV61_03820"
     CDS             complement(<1..1197)
                     /locus_tag="VFV61_03820"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013935707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HEX4899632.1"
                     /translation="MTSKQQPQPAKSKLDKLRERHREAELGGGPERAEKQHAAGKMTA
                     RERIEFLLDEETFEEFDKLVVHRSRDFGLEKQTYPGDGVVTGHGLIDGRRVFVFAQDF
                     TVFGGSLSETHAEKICKVMDLAIKVGAPIIGLNDSGGARIQEGVLSLGGYADIFLRNT
                     LASGVVPQISCTMGPCAGGAVYSPAITDFNIMVKDTSYMFITGPDVIKTVTHEDVTKE
                     ELGGAITHNRVSGVAHFAADSDEHALRMIRELFSFIPSNNLEEAPRLTTNDSAERTAE
                     KLNTLVPEAANQPYDIRDVIGHVVDDGYFFEVQQLYAPNICVGFARLAGRSVGIVANQ
                     PAFLAGVLDIDASVKAARFVRFCDCFNIPLVTFEDVPGFLPGISQEHGGIIRHGAKLL
                     YAFAEAT"
     gene            complement(1200..1685)
                     /locus_tag="VFV61_03825"
     CDS             complement(1200..1685)
                     /locus_tag="VFV61_03825"
                     /inference="COORDINATES: protein motif:HMM:NF016085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEX4899633.1"
                     /translation="MITVRACKYDGSEHRRWPATVVKQEGSLLVLDAAFAEEVNHDLL
                     GTIVSGTRSIEYYWLDRWYNVFRFSQPNGDLRSYYCNVNIPPEFDGQTLSYVDLDIDI
                     LVEPDLTYRVVDLKDFELNAQRYDYSAEVQTNASRALIEVIDLIETRTFPFTDSKISH
                     E"
     gene            complement(1687..2337)
                     /gene="fsa"
                     /locus_tag="VFV61_03830"
     CDS             complement(1687..2337)
                     /gene="fsa"
                     /locus_tag="VFV61_03830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEX4899634.1"
                     /translation="MKFFLDTANLNEISEAAKLGLADGVTTNPSLIAKEGDVDFKEHI
                     AAICKIVSGPVSAEVTSEDTEGMVREGREYAAIAKNVVVKCPLTRAGLMATHQLDGEG
                     IKVNVTLCFSAAQAILAAKAGASFISPFLGRLDDIGENGLVLLGDIIEVYRNYEWKTE
                     VLAASIRHPIHAIEAARMGADIATMPFKVIDQMFNHPLTDKGQAQFLADWRKSGRK"
     gene            complement(2367..3143)
                     /locus_tag="VFV61_03835"
     CDS             complement(2367..3143)
                     /locus_tag="VFV61_03835"
                     /EC_number="3.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX4899635.1"
                     /translation="MKLCRFLHSSSAEPRYGVISGTTVYPVTADEYFNLKEVPRSQAT
                     IALSEIRLLAPVIPSKVVCVGRNYREHAAELGNKMPEEPLLFLKAPSAVIGHEDAIEL
                     PPQSEQVEHEGELGVVIGRTVRRIQPGEDPLTFVLGYTCVNDVTARDLQRKDVQFTRG
                     KSFDTFCPVGPVIVTDLNPLDTTVSTRVNGEVRQQGCTSDMAFPVPFLIKYIADVMTL
                     YPGDLIATGTPAGVSRMKPGDVVEVDVAGVGVLRNQVQSR"
     gene            complement(3150..>3786)
                     /locus_tag="VFV61_03840"
     CDS             complement(3150..>3786)
                     /locus_tag="VFV61_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899636.1"
                     /translation="PSARVAQPPTLSATGKELTESAITINWTPPAANIDGSTPVNLLG
                     YNVYRIDEAQKESGDTPLNSALVSGTQFADQNFEFGQSYQYFVRSVSLGTDGAQVESL
                     NSNSLSIAPRDTFPPSAPSSITIAAAPGRLSIFFPANPEGDVVGYTIYRASDPDLPKE
                     RWTKLTSALLERTTFQDENVESGRRYYYYLTATDKAGNVSAPSEVISEVVP"
BASE COUNT          874 a         1043 c         1022 g          847 t
ORIGIN      
        1 tgtcgcctcg gcaaaagcat aaagcaactt ggcgccgtgc ctgataatgc cgccgtgctc
       61 ttgggagatg ccaggcagaa atccgggcac atcctcaaag gttaccagtg gaatgttgaa
      121 gcaatcacag aagcgtacga agcgcgcggc cttcaccgag gcatcaatat cgagaacccc
      181 ggccagaaaa gcaggctggt tggcaacaat gccgaccgaa cgcccggcca ggcgcgcaaa
      241 tcccacacaa atattggggg catagagttg ttgcacttcg aagaaataac catcgtccac
      301 gacgtgacca atgacatcgc gaatatcgta aggttgattt gcagcttcgg gaaccagagt
      361 atttagcttt tccgcggtac gttctgccga atcatttgtc gttaagcgcg gcgcctcttc
      421 caggttatta gacggaataa aggaaaagag ctccctgatc atgcgcaacg cgtgttcgtc
      481 agagtctgcg gcgaaatgag caacaccgga gactcgatta tgagtgatgg cgcctccaag
      541 ttcctccttc gttacgtctt catgggttac ggtcttgatt acgtcgggac cggtgataaa
      601 catgtaggac gtgtctttca ccatgatgtt gaagtcggtg atggccgggc tgtaaacggc
      661 gccgccggca caaggtccca tagtgcaact gatctgcgga acgacgccgc tcgcgagcgt
      721 gttgcggaga aagatgtctg cgtaaccacc cagacttaga acgccttcct gaattcgcgc
      781 gccgccggag tcgttcaggc caataatggg cgctccaacc ttgatcgcca ggtccatcac
      841 cttgcagatt ttttccgcat gggtttctga aagcgagcca ccgaatacgg tgaagtcctg
      901 ggcaaagaca aagacgcgcc gcccatcgat caggccgtga ccagtaacca ccccgtcacc
      961 gggatacgtt tgtttctcga ggccaaaatc gcgcgaacga tgcacgacga gtttgtcaaa
     1021 ctcctcaaag gtctcctcgt cgagcagaaa ctcaatccgt tcgcgcgcag tcatcttgcc
     1081 ggcagcgtgc tgcttttcgg cgcgctccgg accgccgccc aactcggctt cacgatgacg
     1141 ttcgcgaagc ttgtctagtt ttgatttcgc cggctgcggt tgctgttttg aggtcatgat
     1201 tattcgtggc tgattttgga atctgtgaaa ggaaatgtgc gagtctcaat cagatcgatc
     1261 acctcgatca gggcgcgact ggcattcgtc tggacttctg ccgagtagtc gtagcgttgc
     1321 gcattcaatt caaagtcttt cagatccacc acgcgataag ttaaatcggg ctcgacgaga
     1381 atatcaatat ccagatctac ataactaagt gtttggccat cgaattccgg cggaatgttg
     1441 acgttgcaat aataactgcg caagtcacca ttgggctggg aaaagcgaaa cacgttgtac
     1501 caacggtcga gccaatagta ttcgatactc ctggtgccgc taacaatcgt ccccagtaag
     1561 tcgtggttaa cctcttcggc aaatgcggca tcgaggacca acaaagaacc ttcttgcttt
     1621 acgacggtgg ccggccagcg acggtgctcg gagccgtcgt atttgcaggc tcgcaccgta
     1681 atcatcttac ttgcgaccac tcttgcgcca atccgcaagg aactgcgcct gccccttgtc
     1741 ggtaagtgga tgattgaaca tctggtcaat caccttgaag ggcatcgtcg caatgtctgc
     1801 tcccatgcgc gccgcttcaa tggcatgaat gggatgacgg atgctcgccg ccagcacttc
     1861 agtcttccac tcgtagttgc gatagacctc tatgatgtcg cccagcaaca ccaggccatt
     1921 ctcaccaata tcatccagac ggccgaggaa aggactaatg aaagacgctc cggccttggc
     1981 ggccaggatg gcctgagccg ccgagaagca gagcgtgaca ttcactttga tcccttcacc
     2041 atcgagctga tgcgtggcca tcagtcccgc tcgcgtcagc gggcacttca cgaccacgtt
     2101 cttggcgatg gctgcatact ctcggccttc acgcaccatg ccttcagtgt cttcgctggt
     2161 cacttccgcc gagaccgggc ccgagacgat cttacaaatc gcggcgatgt gctccttgaa
     2221 atcaacgtcg ccttccttcg cgatcaggga tggattggtg gtgacgccgt cggcgagtcc
     2281 caatttggcc gcctcgctga tctcatttag gttagcggta tcaagaaaga acttcataaa
     2341 tgcctcctgc tgcaacgatc ataaaactag cggctttgaa cttgatttcg cagtacccca
     2401 actccggcca cgtcgacttc gacgacgtca ccgggcttca tccgtgacac accggcgggc
     2461 gtgccagtgg cgatcagatc gcccggataa agtgtcatga cgtctgcgat gtatttaatc
     2521 aggaagggaa ctgggaatgc catatcagaa gtgcaaccct gctgcctgac ttcaccgttg
     2581 actcgggtgc taacagtcgt gtcgagggga ttcaaatccg tgacgatcac gggaccgaca
     2641 gggcaaaacg tatcgaacga cttgccgcgc gtgaattgaa cgtctttgcg ttgcagatcg
     2701 cgcgccgtca catcgttaac gcatgtgtac ccgaggacaa aagtcagcgg atcttcgccg
     2761 ggctgaatgc ggcgcacggt gcgaccgata accacgccga gctcaccttc gtgttcaacc
     2821 tgttcagact gtgggggcaa ttcaatcgca tcctcatgac caatcacggc cgatggtgcc
     2881 ttcagaaaaa gcaaaggctc ttccggcatc ttgtttccca actcggccgc atgttcacga
     2941 taattgcgac cgacgcaaac tacctttgag ggaatcactg gcgctagcag gcgaatctca
     3001 ctcaacgcga tcgtggcttg cgagcgaggc acctctttca gattgaaata ctcatctgcc
     3061 gtgaccgggt aaaccgtagt acccgagatg acaccgtagc gtggctcagc cgagctcgaa
     3121 tgaaggaatc gacaaagttt catcgcgcgt cagggaacga cttcagagat tacttccgat
     3181 ggagcactta cattgcctgc cttgtcggta gcggtcagat aatagtagta gcgtcgaccg
     3241 gactcgacgt tctcatcctg gaaggtcgtt ctttccagca aggctgaagt tagtttcgtc
     3301 caacgttctt taggtaagtc gggatcggaa gcacggtaga tcgtgtaacc aacgacgtct
     3361 ccctccgggt ttgccgggaa gaaaatcgat aaccggccag gtgccgccgc aatggtgatc
     3421 gacgaaggcg ccgaaggagg gaaggtatcg cgcggcgcga ttgagagcga attggagttg
     3481 agactctcaa cctgagcgcc atccgttccc agtgaaaccg aacggacgaa gtactgatag
     3541 ctctggccaa actcaaaatt ctgatccgcg aattgagttc ccgaaacgag cgcgctgttc
     3601 agtggcgtat cgccactttc tttttgcgct tcatcgatgc ggtaaacgtt atagccgagc
     3661 agattcactg gggtagatcc atcgatgttt gcggcgggcg gcgtccagtt gatcgtgatc
     3721 gctgattcag tcaactcctt accagttgca gataaggtgg gtggttgcgc cacgcgcgct
     3781 gatggc
//
LOCUS       DASUEX010000227         3078 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1414581, whole genome shotgun sequence.
ACCESSION   DASUEX010000227 DASUEX010000000
VERSION     DASUEX010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3078
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1414581"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="VFV61_03845"
     CDS             <1..265
                     /locus_tag="VFV61_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX4899637.1"
                     /translation="GLKGDARAVEPLRAALNDPDSDVRKQARWALELRDLKMGKRIKI
                     NKKNIDADVDSDVDVDVDVNVNVDPKIKVKVKPDPKPKAKADF"
     gene            complement(340..951)
                     /gene="purN"
                     /locus_tag="VFV61_03850"
     CDS             complement(340..951)
                     /gene="purN"
                     /locus_tag="VFV61_03850"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEX4899638.1"
                     /translation="MPDTEKVWRLGIIISGRGSNMVALADAVSAGRVPHTEVALVVSD
                     QANALGLERARERGLETIVIERRGRSREDHDREIAAALRERRVDLVCLAGYMRLLSPE
                     FIAAFRNRILNIHPSLLPDFPGLHPHKQALDAGVTSSGCTVHYVDETLDGGPIIAQRR
                     VPVLPEDTEESLAARILKQEHELYAEAVAEVIATAKGGKKVKG"
     gene            complement(1038..>3078)
                     /locus_tag="VFV61_03855"
     CDS             complement(1038..>3078)
                     /locus_tag="VFV61_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899639.1"
                     /translation="ENPKKLIVCTSAPHTAFGKFADKDDEKSAKAFWQLGLPRPFLRS
                     ENESVPAQQAEGEPPVEKEGLKDDEIRLDLAGDVHHYARYWGPASKNTQPTREHLPPR
                     EPLAKANYASVMSGLGGAFHHPSTTSEYEITEQALYPNEDQSRKYVAEQIFNPWRVVK
                     GGGVWFVGGLVALILSFAAIADRSTRPAIHNFKPFIALGITQPERYNSTVESESEAKS
                     QQASIQHNVFTNALRDIGLVKQQWTPTPSSAPECQTPAELAKPLFLWGKCRTVWPAEY
                     AWGFWLFISTVPVMLIGILVMRWYYQRYKEAQENSPGSGDADENKRHVLYVNLLIWGF
                     TIFTTLQALAAVLIVMPYRSFITPFGNSLLVLLTFCWAGFAIYLSLKYSDWLFEQTSQ
                     RAIRRRDWAITWVLAACAFFAVGIGLWVFGKANPAAYLVADIISVLVVVGAPLLLLIA
                     GWKVGGAHQKGFGKVGMGLIGIWHGLLQIGVAVFLIKKGTWLTVGLAIVLVVIFWQVG
                     RAMMRSNLRRLLTVAWFLFGALMLALPPLVYYILINNASYPPTDEWKFFFWPHTVDPA
                     LPFSSYEWWSSWTGWWQLVPIFLGGVFGAFLSCTWLGWYFGVCLGFHGHNNEVGGAAR
                     IEEFKQLIRFRLTPDTLTGYVIGVRDPKRCGRFLDPYLIDIFQLTYPKKEGAGET"
BASE COUNT          771 a          851 c          790 g          666 t
ORIGIN      
        1 gggactgaag ggcgatgcac gcgcagtgga accgttgcgc gctgctttga acgatccgga
       61 ttcggacgta aggaaacaag cacggtgggc tttagaactt cgcgatctga agatgggtaa
      121 acggatcaag atcaacaaga aaaatatcga cgctgatgtg gattcagatg ttgatgttga
      181 tgtcgacgtg aatgtgaacg tggaccctaa gataaaggtg aaggtaaagc cggatccaaa
      241 accgaaggcg aaggcggact tctgaattaa aggggaaggg gtaaaagggc aaatggggaa
      301 gattactctt gggtttttcc ctcttcccct tttgcccttt caccccttaa cctttttacc
      361 ccctttcgcc gttgcgatca cctccgccac cgcctccgca taaagttcgt gctcctgttt
      421 tagaatccgt gccgctaaag actcttcagt atcttccgga agcaccggca ccctcctttg
      481 cgcaatgatg ggccctccat caagcgtctc atcgacatag tgaactgtgc agccactcga
      541 agtgaccccg gcgtcgagtg cttgtttgtg gggatgaagg ccaggaaaat ccggcaacaa
      601 actaggatgg atgtttagaa tgcgattgcg aaaagcggca atgaactctg gcgaaagcag
      661 gcgcatataa ccggccagac aaaccagatc aacccggcgt tcacgcagcg ccgcggcgat
      721 ctcgcgatcg tgatcttcac gactgcggcc gcgacgctcg attacgatcg tttccaaacc
      781 gcgttcgcga gcgcgttcga gtccgagtgc attggcttga tcgctaacca ccaaagccac
      841 ttcagtgtgc ggaacgcgtc cggcactaac cgcatcggcc aacgccacca tgtttgagcc
      901 gcgaccggag atgatgattc cgagacgcca caccttctca gtgtcaggca agtagatccc
      961 tcttgagagt ttccgttttc aacccgagcc tcacagatgc gaaagcgact ggagtgtgta
     1021 gaactcttca acctacgcta agtctcaccc gctccctctt tctttggata tgttagctgg
     1081 aaaatatcaa tcagatacgg atccaggaaa cggccgcacc tcttaggatc tctgacgcca
     1141 atgacatatc cggtcagcgt gtcaggagtg aggcggaaac ggatcagctg tttgaactct
     1201 tcaattcgtg ccgcacctcc aacttcgttg ttgtggccat gaaaacccag gcaaacgccg
     1261 aagtaccaac ctagccaggt acagcttaag aacgctccaa aaactccgcc aagaaagatc
     1321 ggaaccagct gccaccaacc ggtccagctc gaccaccatt cgtaagatga aaatggcagc
     1381 gcagggtcga cggtgtgagg ccagaaaaag aatttccact cgtctgtggg gggataggag
     1441 gcattgttga ttaggatgta gtaaacgagc ggcggaagcg ccagcattaa cgccccgaaa
     1501 agaaaccagg caaccgttag aagccggcgg aggttgctgc gcatcatggc tcgtccgacc
     1561 tgccagaaga tcacaaccag gacgattgct aagcccactg tgagccacgt gcccttctta
     1621 atcagaaaga cggcaacccc gatttggagc agtccatgcc aaattccgat caaccccata
     1681 ccaacttttc cgaacccctt ctgatgagca ccacccacct tccaccctgc aatcagcagt
     1741 aacaacggag ccccaacgac cacaagcacc gaaatgatgt cagccacgag atatgccgcc
     1801 ggattggctt tgccgaatac ccaaagcccg atacccacag caaagaacgc gcacgctgcc
     1861 agaacccagg taatcgccca atcgcggcgg cgaatcgccc gctgggaggt ttgctcaaac
     1921 agccaatcgc tatacttaag actcagatag atcgcgaaac ccgcccagca gaacgtgagc
     1981 agtaccagga gactattacc gaaaggtgtt atgaagctgc ggtaaggcat cactatcaaa
     2041 accgcggcca gtgcctgcag agtggtgaaa atcgtgaatc cccagatcag caaattcacg
     2101 tataggacgt gccgcttgtt ttcgtcagca tctccagagc caggactatt ctcttgtgct
     2161 tctttgtacc tttggtaata ccagcgcatt acgagaatac caatgagcat taccggaacg
     2221 gttgagatga aaagccagaa accccaggca tactcagccg gccatacagt tctgcacttg
     2281 ccccaaagga ataacggctt agcaagctct gctggggttt ggcattccgg cgcacttgag
     2341 ggcgtaggag tccactgctg cttcaccagg ccgatatctc gcagcgcatt ggtgaacacg
     2401 ttatgttgaa tactggcctg ttggctcttc gcttcagact ctgactctac agtcgagttg
     2461 tatcgctcgg gctgagtgat cccgagagcg atgaacggct tgaaattgtg aatggcaggg
     2521 cgcgtacttc gatcagcaat tgccgcgaac gacagaatca aagcgaccaa accgcccaca
     2581 aaccaaactc cgcctccttt tactactcgc cagggattga agatctgttc cgccacgtat
     2641 ttgcgcgact gatcctcgtt gggataaagc gcttgctccg taatttcgta ctcagatgta
     2701 gtcgagggat gatgaaaggc gccgccgagt ccggacatta ctgaagcgta gtttgctttc
     2761 gctaaaggtt ccctgggtgg cagatgttct cgcgtcggtt gcgtgttttt tgaagctggt
     2821 ccccaataac gcgcgtagtg atgcacatcg ccggcgaggt cgagtctgat ctcgtcgtct
     2881 tttagtcctt ccttttcaac tggcggctca ccttcagcct gctgcgccgg tacagactca
     2941 ttctcagatc gcaagaaagg tcgcgggagg ccgagttgcc agaaagcctt cgctgatttt
     3001 tcgtcgtctt tatccgcaaa ctttccaaac gcggtatgcg gcgcgctagt gcagacaatc
     3061 agcttcttag gattttcg
//
LOCUS       DASUEX010000228         2018 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_848184, whole genome shotgun sequence.
ACCESSION   DASUEX010000228 DASUEX010000000
VERSION     DASUEX010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_848184"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="VFV61_03860"
     CDS             complement(<1..284)
                     /locus_tag="VFV61_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899640.1"
                     /translation="MRLLLLSLSVLTIGLVSVAVFSQRQKGSVTRAPSTPVNVNGHPV
                     DGIDATDYGADVREITLEDLKKLLQRDPKDTRPVLLNFWATWCDPCREEFP"
     gene            complement(341..955)
                     /locus_tag="VFV61_03865"
     CDS             complement(341..955)
                     /locus_tag="VFV61_03865"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX4899641.1"
                     /translation="MKKHFTVYLLLASVLVIALAAVAGNGRSSTDIPAPPAIGSVIDD
                     FTLADADGKEHSLKSLAGPKGVVILFVATKCPVSNAYNERMEKLAQDYKAKGINVVGI
                     NSNNTEPAAEVKSHAADKGLTFVILKDDGNKIADRFGATKTPEAYVLDASMKLVYHGR
                     IDNSQKVESITANDLRDALEELLTGKAITKTGGAAFGCTIKRVS"
     gene            complement(1050..1508)
                     /locus_tag="VFV61_03870"
     CDS             complement(1050..1508)
                     /locus_tag="VFV61_03870"
                     /inference="COORDINATES: protein motif:HMM:NF019499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HEX4899642.1"
                     /translation="MRSPQTAAHSSPTAIPWTYLSAALLALIGLGDAVYLTIQHITGA
                     SLRCTVISGCSEVLSSPYAQVGPMPLAAIGAAAYFVVFSFAILAAFSYGFAIPLLRIV
                     VALMFLTTLWLLYLQAFVIKHFCQYCLLSAAVTIAVVAILFLPALWRRRR"
     gene            complement(1548..>2018)
                     /locus_tag="VFV61_03875"
     CDS             complement(1548..>2018)
                     /locus_tag="VFV61_03875"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX4899643.1"
                     /translation="ACGVYYPELKKIESEFGASLKVIFRENPLYPNHEYSIIAAQAAE
                     AAGLQGEAQFWAMHDKLYENQKTWAETKDRDAVVSLFAGYATEIGLNTDRFMRDLNGE
                     AVAQRIFQDGKRSHALGISQTPTFVVNGKEATGESWKPDGLRQMINEALREAGQ"
BASE COUNT          432 a          560 c          586 g          440 t
ORIGIN      
        1 ggaaactctt cgcgacacgg gtcgcaccat gtagcccaga agttaaggag caccggccga
       61 gtgtctttag gatcgcgttg cagcagcttc ttgaggtctt cgagagtgat ctcgcggacg
      121 tccgcgccgt aatccgtagc atcaatgccg tcgaccgggt gcccgttcac attgacggga
      181 gtactcggag cgcgcgttac gcttcccttt tgccgttggg aaaacacggc gacgctcacc
      241 aatccaattg tcaaaacact gagagacaaa agaagcagac gcattttcat gtggcgcagg
      301 cctcgcgcag ttgcatttca aatcggcggg caaaccgtcg ctacgaaacc cgcttaatgg
      361 tgcagccgaa tgccgcgcca ccagtcttcg taatggcctt gccggtgagc agctcctcga
      421 gcgcgtcgcg tagatcgttc gcagtgatgc tttcgacttt ttgcgaattg tcgatgcggc
      481 cgtgatagac gagcttcatg ctcgcatcga gtacgtatgc ttctggagtc ttggtcgcac
      541 cgaaacgatc agcgatctta ttgccatcgt ccttgagaat cacgaacgtc aggcctttat
      601 cagcggcgtg tgacttcact tcggcggccg gctcggtatt gttcgagttg atgccgacta
      661 cgttaatgcc cttcgctttg taatcctggg cgagtttttc catccgctcg ttgtaagcgt
      721 tcgagaccgg gcacttggtc gcgacgaaaa ggattacgac gcctttcggt ccggccaggg
      781 acttcagcga gtgttcttta ccgtcagcat ccgccagtgt gaaatcgtcg atgacactgc
      841 caatcgcggg cggcgcgggg atgtcggtcg agctgcgccc gttgccggca actgccgcaa
      901 gcgcaattac caaaacagaa gcgaggagca gataaaccgt gaaatgcttc ttcatgcgga
      961 actcctttcg agcggtgaat tgaagtcaga gattaacaag cgttggttag acgtggcaag
     1021 cggccgtttg gttcgcgcgc cgacgaactt caccgtcgtc gtcgccacag cgcgggcagg
     1081 aaaaggatcg ctacgacggc gatggtgact gcggccgaca acagacagta ctggcagaaa
     1141 tgcttgataa cgaaggcttg caaataaagc agccagagtg tggtcagaaa cattagcgcc
     1201 acgacgatcc tgagcagcgg aatcgcgaag ccatagctaa atgccgccag aatcgcgaag
     1261 ctgaaaacta cgaagtaggc ggccgcgccg atggccgcaa gcggcattgg accgacttgc
     1321 gcgtacgggc tgctgagaac ttcagagcag ccggaaatga ctgtgcagcg aaggctggcg
     1381 ccggtgatgt gctgaatcgt cagatagact gcgtcaccga ggccgatgag cgccaggagt
     1441 gcggcgctca aatatgtcca gggaatggca gttggcgaag aatgcgctgc ggtttgtggt
     1501 gatctcattc aagatcaacc gcccgctccc gcaggcggtt ttgactccta ctgcccggcc
     1561 tcacgcaacg cttcattaat catctgccgc agaccgtcgg gtttccacga ttcgccggtg
     1621 gcttctttgc cgttgacgac aaatgtcggc gtttgactga tgccaagagc gtgcgagcgt
     1681 tttccgtcct gaaagatccg ctgcgctact gcttcgccgt tcaggtcacg catgaaccgg
     1741 tcagtgttga ggccaatctc ggtggcgtag ccagcaaaga gcgaaacaac cgcgtcccga
     1801 tctttcgttt ccgcccaggt cttctgattc tcgtaaagct tatcgtgcat cgcccagaac
     1861 tgcgcctcgc cttggaggcc ggccgcttcc gccgcctgtg cggcaattat cgagtattca
     1921 tgattcgggt acagcggatt ctcacgaaag atcactttca agctggcgcc gaactcgctt
     1981 tcgatcttct tgagttcagg gtaatagacg ccgcaggc
//
LOCUS       DASUEX010000229         2438 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1169567, whole genome shotgun sequence.
ACCESSION   DASUEX010000229 DASUEX010000000
VERSION     DASUEX010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1169567"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1995
                     /locus_tag="VFV61_03880"
     CDS             <1..1995
                     /locus_tag="VFV61_03880"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899644.1"
                     /translation="DAKWERAVEGVLGSSLQSIVVPTPDDAARAAQWLRENNAGRASF
                     LVAGLHGASDETDTLACRIEERPALSLSFSSEGLEDLFIRDLLGAPRELVAVLNRTLP
                     QKMNARLATSLDDAMAKSLATGEVYVTLNGDWVAQGQFVSAGDARALEEGAGLLAFKR
                     ELRELEGRAEVLQSQLLSAESAAAEARARLIGLEDAVVLLNESIGREEREVMTRELTA
                     AGLAQEIERAERHIRVVAADTARVEEERVQVERRRLDALADADAAEAARSRATETVTN
                     ASAVLAEARREAEQENQGLSQQRAIAAAAAERRRATATELRRLEGEYADVAARVERHR
                     LEVVELTASIEMLRTSIAELTRLAETVAEEQAQEEQQIADFTLRLEEARKAADTLAAE
                     LSELNRRAAEIRDSRAELEVQRAEAQARLTFVRESCAAELNQPLEDLAREQVFDAEFD
                     LTTAQARVDELRSRLDSFGAVNMMALEELTEAEERLVFLTGQRTDIVDSISSTEEALR
                     EIKRRSRERFRHAFEQINKNFSELFQELFGGGRGEMSLIEADDVLESGIDVIAQPPGK
                     RLQNVLLLSGGEKAMAALALVLGIFHYRPSPFCLLDEVDAPLDEANVGRFTDKVVQMS
                     ANTQFIVITHNKRTMEMARALYGVTMEEVGISKLVSVKFE"
     gene            2101..>2438
                     /locus_tag="VFV61_03885"
     CDS             2101..>2438
                     /locus_tag="VFV61_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899645.1"
                     /translation="MINQYLFRHFLPAALAILSAAIFISASELRGPRGAGGLHYGLSL
                     SQESGSKRNARPGEPAQNQAAGNIPKPKAVNARYFYEFSQPQFYVRKVRIEHDSNGRG
                     KITFERLNEDL"
BASE COUNT          533 a          624 c          783 g          498 t
ORIGIN      
        1 gatgcgaaat gggaacgtgc cgttgaagga gtcctgggct cctctttgca gtcgattgtt
       61 gttcccaccc cggatgacgc cgcgcgcgca gcgcaatggc tgcgagagaa caatgctggt
      121 cgagcaagct ttttggtggc aggcctgcat ggcgccagcg atgagactga cacactggcc
      181 tgcagaatcg aagaacgccc tgcactctca ttgtcattct cgagtgaggg actggaagac
      241 ctctttattc gcgatctgct cggtgctccg cgagagctgg tggccgtctt aaatcggacg
      301 ttgccacaga agatgaatgc caggctggcg accagccttg acgacgcgat ggccaagtcg
      361 cttgcgaccg gtgaggttta tgtaacgctg aacggcgact gggtggccca ggggcaattt
      421 gttagcgccg gggatgcgcg tgctctcgaa gaaggcgctg gcttgcttgc cttcaagcga
      481 gaattgcgcg agctcgaggg gcgagcagag gtactccaga gccagttgct gtccgccgaa
      541 tcggccgccg ccgaagcacg tgcgcgcctg attggattgg aagacgcggt cgtgttgttg
      601 aacgaatcga tcggccgtga agaacgtgaa gtgatgactc gggaattaac tgcggcgggt
      661 ttggcccagg agattgagcg cgccgagcgt cacatacggg ttgttgctgc tgataccgcg
      721 cgtgtcgagg aagagcgggt ccaggttgag cgccggcgat tggacgcact tgctgatgca
      781 gacgctgcgg aagcggcgcg tagccgggct actgagactg tgacaaacgc ctccgctgtg
      841 ctggccgaag cacgccgaga agccgaacaa gagaatcagg ggttatcgca acaacgcgcg
      901 atcgctgcag cagccgcgga aagacgccgg gcgacagcta ctgaactgcg ccggctggaa
      961 ggtgagtacg cggatgttgc cgcgcgcgtg gaacgtcatc ggcttgaagt ggttgagctc
     1021 acggcaagca tcgaaatgct ccggacctcg atcgcggagt taacccgttt ggccgaaacc
     1081 gtcgcggagg agcaggcgca ggaggagcaa caaattgcag actttactct gcgtttggag
     1141 gaggcgcgta aggctgccga cacccttgct gccgagctct ccgaactgaa ccgtcgtgct
     1201 gccgaaatac gcgattcaag agccgagctc gaagtgcaaa gggcggaggc gcaggcgcgt
     1261 ttgacgtttg tacgcgaaag ctgcgccgcc gaattgaatc agccgttaga agatttggct
     1321 cgggagcaag tgttcgatgc ggaattcgat ctaaccacag ctcaggcgcg cgtggacgag
     1381 ctccggtctc gcctggatag cttcggcgcg gtcaacatga tggcgttgga agagctgacg
     1441 gaagccgagg agaggttggt cttcctcacc ggccaaagga ccgatattgt cgatagcatt
     1501 tcttcaacgg aagaggcctt gcgcgagatc aaacggcgct cacgggagcg ttttcggcat
     1561 gcgttcgagc agatcaacaa gaactttagt gagttgttcc aggagctttt tggtggtggc
     1621 cgtggcgaga tgagtttgat tgaagccgac gatgtgctgg agtccggtat tgatgtgatc
     1681 gcgcagcctc ccggcaaacg tttgcagaat gtactgctgt tatctggtgg tgaaaaggca
     1741 atggccgccc tggctttggt gcttggcatt ttccactatc gcccgtcgcc attctgtctg
     1801 ctggatgaag tcgacgctcc gcttgacgaa gcgaatgttg gccgctttac ggacaaagtt
     1861 gtgcagatgt cggcgaacac ccagttcatt gtcatcacgc acaacaagcg aacaatggag
     1921 atggcgcgcg ccctttacgg cgtgacgatg gaggaagttg ggatttcgaa actcgtctca
     1981 gtcaagtttg aatagggctc tatcagaaac gtacaggagc gcagaggagt aagcgcagga
     2041 aaaaacaatt cagcgggcga tcggttgacg taatccagct ttcagtcgta tgaatctcaa
     2101 gtgattaatc agtatctgtt ccggcacttt ctccccgccg cgcttgcaat actaagcgcg
     2161 gcgattttca tctccgcgag cgagcttcgc ggtccgcgcg gggctggcgg attgcactat
     2221 ggcctctctc tttcgcagga gagtggaagc aaaaggaatg ctcgccccgg agagccggcc
     2281 cagaatcagg ccgctggcaa tatccccaaa ccgaaggccg ttaatgcgag gtacttctac
     2341 gaattcagtc agccgcagtt ttatgtcagg aaagtccgga ttgaacacga ctcaaacggt
     2401 cggggcaaga taacgttcga gcgtctcaac gaagacct
//
LOCUS       DASUEX010000230         2130 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_964320, whole genome shotgun sequence.
ACCESSION   DASUEX010000230 DASUEX010000000
VERSION     DASUEX010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_964320"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(645..1895)
                     /locus_tag="VFV61_03890"
     CDS             complement(645..1895)
                     /locus_tag="VFV61_03890"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX4899646.1"
                     /translation="MNYFLATATGDDTRVLLTLFIMLVAAKLIAELFERLRQPAVAGE
                     ILAGVVIGPSLLGWAAPSEITSLLAEIGVIFLLFTVGLETKPAAIFKVGKQAAVVAVL
                     GVVAPLLGGWLLMRAWGSTSIEALFVGTAMVATSVGITARVLSGMGLLDAPTARIILG
                     AAVIDDILGLLVLAIVSSMAAGTVNYVEILTTAGLAIGFTAFVALVGAPVVTRVAPSV
                     ERMRSGQGIFIFGLVLCLGLSVAATYIGVAAIIGAFLAGMALAEAAEDNPKMHRQMSG
                     VTEFLVPFFLVNIGMQLSLGVFRSSSVILLCVLVTLVAIATKLLGCGLGAFNLGTRRA
                     GQVGMGMVPRGEVGIIVAQIGLSLAVIGAELYGVVLFMAVATTVIAPPFLKLLYASEV
                     AARKEIGPPDAGGIVASEDLCQIG"
BASE COUNT          523 a          626 c          524 g          457 t
ORIGIN      
        1 gtagcgctca acaatgtgac gaaacgcctc attgtctccg cttctgcatc tttcaatcgc
       61 cgagtgatca tccatcgttc agctctctca cctaacgtat ccctgcagga gccaaattat
      121 tcaaaaaatt tcccggacga ggaaattctt gtttcgactc cctaaattaa ggctgttcag
      181 gaacccactt ctctaactgt ttggttaaaa ggtccgacgt tccttcaaag gaaccagtga
      241 gctcgaataa gaggtttcgc tttgcctgtg tggagtgcaa ccccaggccg agttttcgaa
      301 aatcctgatg agttagatgt cacgtgagag ccgaacaagc atctggcttt tgggcaaggt
      361 gttcactttt gcctgggagc tcctctagcc cggttggacg gagggattgc aatcaacaca
      421 ctcttacggc gaagtccgga tctgcgtctg agcgttgaga gcaaatctct acgccggcgt
      481 ccgagcatgt ttttgcgtgg acttgaatcg ttacccgtat ctttcgacgt tgcggttcgt
      541 tgaggtggtg gcgcgcctct catgaaccac tctccagttc gcgctcagca aaaccaatat
      601 tgaagcgagc ggctttatca tcgacgcgtc ttatcctggg tgtctcaccc aatctggcaa
      661 agatcttctg aagctacgat accgccagcg tcgggcggtc caatctcttt ccgcgcagca
      721 acctcactag cgtaaagcaa cttgaggaat ggcggcgcaa tcacagtcgt ggctacagcc
      781 atgaacaaga caacgccgta gagttctgct ccaatcaccg caaggcttaa accaatctgg
      841 gccacgatga ttccgacttc gccgcgcggc accattccca tacccacttg tcctgcgcgt
      901 ctcgttccga gattgaaggc gcccaaaccg caccctaaca acttggtcgc aatcgcgacc
      961 aaagtcacca gcacgcaaag caggatcact gaagaagaac gaaagacgcc gagactaagc
     1021 tgcataccga tgttgacgag aaagaagggc acgaggaatt ccgtcacgcc actcatctgc
     1081 cggtgcatct tcggattgtc ttcagcggct tccgcgaggg ccatccctgc gagaaaggca
     1141 ccgatgatcg cggcaactcc gatgtaagtt gccgcgacgg atagacccaa gcacaacact
     1201 agcccgaaga tgaagatacc ctgcccactg cgcattcgtt caacgcttgg cgccacgcga
     1261 gtaacgacag gcgctccaac cagagcgaca aaagcggtaa acccgattgc cagacctgca
     1321 gtggtaagga tctcgacgta attaactgta cctgctgcca tactggaaac aatcgcaaga
     1381 actaaaagcc ccaggatgtc atcgatgact gctgcgccca gaatgatgcg cgccgtaggc
     1441 gcatctagta gtcccattcc cgataacacc cgcgctgtaa tgccgactga tgtcgctacc
     1501 attgccgtac caacgaagag cgcttcaatg ctcgtactac cccaggcccg catgagtagc
     1561 cagccaccga ggagtggagc aaccaccccc agcactgcca cgactgccgc ctgcttgccc
     1621 accttaaaaa tggctgccgg tttggtttcc aacccgacgg taaacaggag aaaaatgacg
     1681 ccaatctccg ccaggagact ggtgatttct gagggagctg cccaacccag aagacttggc
     1741 ccgataacga ctccggccag tatctcgcca gcgactgctg gttgacgaag tcgctcgaac
     1801 aattcggcta tcagcttggc cgcgacgagc ataatgaaga gggttagaag aaccctcgta
     1861 tcatctcccg tcgcggtcgc cagaaaataa ttcatacctt cacccatcgt gataccggaa
     1921 ccgtcatcga cgtcttaaat tcaaagccaa gctcgccgca atcattcaca taactgcggc
     1981 gagctggttc accgtccttg agacatacct tttccatacc caaggtgttt cgcgtaaggg
     2041 ccgacgcgaa cgctaacccg gctgaccctc tcgatgagag tcaggaggtt tgattgtggt
     2101 gcgttgacgg tgtttttcac ctcgtcggct
//
LOCUS       DASUEX010000231         5424 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1170010, whole genome shotgun sequence.
ACCESSION   DASUEX010000231 DASUEX010000000
VERSION     DASUEX010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5424
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1170010"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1839
                     /locus_tag="VFV61_03895"
     CDS             <1..1839
                     /locus_tag="VFV61_03895"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HEX4899647.1"
                     /translation="NKLSAHVDLTKPIRLDDTSAPGLDQKVRFLAASLRTRSLLVVPV
                     VLGGDVLGLVGMHTTRSTRAWLDDEVSFLESIASQIAVGYQYTRLFTDKEREGETKRA
                     LLEIANALNARSDFRDVSSLVLERAIALVGADYSALGVLDRQGGRISLAAFKAAPHAT
                     TDTVQGLIASHEQSLDVTEFPAAMAVLAEGKTLRLLDKDLPFPFRLIFNATLGGRAAL
                     VAPVRVAGHTFGLLGLVWSEAREGFDDHEVALVEGIADQIGTALERDQLSAEVMHLRS
                     ALHERYGEDRIIGQTAAIRHAMELALSVANTQTTVLIHGESGTGKELLANLIHYNSGR
                     ENHPYVKLNCGAIPETLLESELFGHEKGAFTDARTRRRGRFEEANGGTLFLDEVGEMS
                     LSAQVRLLRVLQDGEFTRVGGSEVLKTDVRVIAASNVDLERATEQGTFRRDLYYRLSV
                     FPIVLPPLRERPEDIHPLVIHFLEHYKQKTGRFISGISKDALQALMIYHWPGNVRELE
                     NSIERAVIIASGRQIELEDLPEAINKTVLEERDRIKVERASAASEGRTTTFAVTVPSS
                     MEEIERQAIEATLDYTRGDKSHAARALGIGRKTLYRKLEQYNGKPE"
     gene            2243..2710
                     /locus_tag="VFV61_03900"
     CDS             2243..2710
                     /locus_tag="VFV61_03900"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEX4899648.1"
                     /translation="MGSDDYRQDRGDSVSTRLTYLLIGGGIGAVIALLFAPKSGQELR
                     EDIADATRKGIDRSREAAQQLGERAGEYYETGRERAAELYSTASERVGDAARTARDTA
                     SDLARTARETAARQTGTVAAAIDAGKKAYQEEKRKTEMSGRIEAAPKYDEKAN"
     gene            2812..3381
                     /locus_tag="VFV61_03905"
     CDS             2812..3381
                     /locus_tag="VFV61_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899649.1"
                     /translation="MTLALQFNTQDPMFWVMVVIALSFIIMAIAMVSIAVFVSRAVKS
                     VNRLEEKLEPLIEKVTVVSEQGKQIAIQGKHIAEQITAVSSHLSTATMHFSESLAILK
                     DEVGELKEVVSHGAREARDKVELVSNTIDRTHRQVTTTTDFIQSKVIEPARELAAIMA
                     GFRRGLEVLVAPAPKPINQIYGEDEMFIG"
     gene            3551..>5424
                     /locus_tag="VFV61_03910"
     CDS             3551..>5424
                     /locus_tag="VFV61_03910"
                     /inference="COORDINATES: protein motif:HMM:NF012361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX4899650.1"
                     /translation="MSTETLDLKKTVNLPKTGFAQKANLAQNEPARLKKWSELKLYDL
                     IRQDRACAEKFVLHDGPPYANADIHLGTALNKILKDFIVKSRTMMGFDAPYVPGYDCH
                     GLPIELYVDRKLGAKKANMPPVSIRRACREHAAEALKRQTRDFQRLAIFGEWENPYLT
                     MSNHYEAETARLFGRFVERGYVYKGARPVYWCIHDQTALAEAEVEYHQHSSPSVYVKF
                     PLRSDPALIDPALAGRKVFVLIWTTTPWTLPANLGIAVHPDFEYSAFAHGDEVFIVAS
                     ELLESVAEKCGLTATGAEPLQVLARFPGARLDRLECGHAWLDRASLLMVGDHVTLGGE
                     ADAEVELDVREARDPKKTGKSGTGCVHTAPGHGHDDFVLGKSYGLEIYCPVDNSGHFT
                     PEVEHFAGQSVFAANPKIVEFMRERGVLLFTETYEHRYPHCWRCKNPVIFRATPQWFI
                     SMDRAGGPVVERDEDGRDRSNFTDNFSDASSSGANSLRVTALAEIERVQWLPAWGLDR
                     MRNMFKGRPDWCVSRQRVWGVSIPVFYCAKCNEAVADPQIINRVADVFEKESADAWYT
                     RTASELLPEGYKCGNCGEQDWIKETDILDVWFDSGSSSIAVLEHRPELRWPADVYIEG
                     G"
BASE COUNT         1213 a         1498 c         1530 g         1183 t
ORIGIN      
        1 aacaagcttt cggcgcatgt tgacttaacc aagccgatac ggttggacga cacgtcggct
       61 cccgggctcg atcagaaagt tcgcttcctg gcggcgagct tgcgcacacg atcgttgctg
      121 gttgtgccag tagtcctggg aggcgatgtg ctcggcctcg tcggcatgca tacgacgcgt
      181 tcgacgcgcg cctggctcga tgacgaggtt tcttttcttg aatccatcgc cagccagatt
      241 gcagtcggct accagtacac acggcttttc accgataagg aacgcgaggg cgaaacaaaa
      301 cgcgccctcc tcgaaattgc caatgctctt aacgcgcggt cagactttcg cgacgtttcc
      361 tccctcgtgc tcgaacgcgc gatcgcactt gtcggagcgg actattcggc tctgggagtt
      421 ctcgatcgcc aaggcggacg cattagtctg gcagcgttta aggccgcgcc acatgccact
      481 actgacacgg tccagggatt gattgcttcg cacgaacagt ctttggacgt caccgagttt
      541 cccgcggcca tggcggtgct cgccgaaggt aagacgctgc ggttgttgga taaagactta
      601 ccgtttccct tccggctgat tttcaatgct accctcggtg gccgcgccgc cctggttgcc
      661 ccggtgcgtg ttgccggtca cacatttggt ctgctgggcc tcgtttggag tgaagcgcgc
      721 gaaggatttg acgatcacga agtagcgctg gtagagggga tcgcggatca aatcggtaca
      781 gcgctggaac gcgatcaact ttcagcggaa gtcatgcacc tgcgcagcgc tctgcatgag
      841 cgttatggcg aagaccgcat cattggacag actgcggcga ttcgacatgc gatggaatta
      901 gcgctcagtg tggcgaatac ccagaccacg gttttgatcc acggcgaatc aggaacgggt
      961 aaggagcttt tggcgaatct aattcattac aactccgggc gcgagaacca tccctacgtg
     1021 aagcttaatt gcggcgcgat tcctgagacg ctactggaat cggagttgtt tggacacgag
     1081 aaaggtgcgt tcaccgacgc acgtacgcgg cgtcgcggaa gatttgaaga ggcaaatggc
     1141 ggcactttgt tcctcgacga agtaggcgag atgagtttat cggcgcaggt tcgtctgcta
     1201 cgcgtgcttc aggacggcga gttcacacgc gttggtggca gcgaagtatt aaaaacggat
     1261 gtgcgagtga tcgccgcttc aaatgtggat cttgaacgcg ccactgaaca gggaacattc
     1321 aggcgtgatc tttactaccg actttcggtc tttccgatcg tgcttccgcc cttgcgcgag
     1381 cgccctgaag acattcatcc gctcgtgatc cattttcttg aacactacaa acaaaagacc
     1441 ggacgtttca tttccggtat ttccaaagac gcattgcagg ctctaatgat ttatcactgg
     1501 cccggcaatg ttcgggaact ggaaaactcg attgagcgcg ctgtaatcat cgcttcgggc
     1561 cggcagattg aactcgaaga cctgcccgaa gccattaaca agactgtcct ggaagaacgc
     1621 gatcgcatca aggtggaacg cgcgagcgcc gccagcgagg gacgcacgac gacgtttgcg
     1681 gttacggtcc cttcatcgat ggaagaaatc gaacggcagg caatcgaagc cacactcgat
     1741 tacacacgcg gggacaaatc ccacgctgcc cgtgcacttg gtattggtcg caagacgctc
     1801 taccgaaaat tggagcagta caacgggaaa ccggaatagt ctcaagtccc aagtctcaag
     1861 tcccaagtct caagtctcaa agtctcaagt ctcaaagtct caagcatttc accgccagga
     1921 cgacaagtcg cagagacgcc aagaaagctt ggacaactga tcgctggttc gtgatttgta
     1981 ctatctatct tgatttgtac tatctaaagg cctaactgct agatctgcct gccaggcttt
     2041 cctttgtgtc ttggcgcctt ggcgtctcgg cggtgaaatg cttgagacct cagacttgag
     2101 accttagacc tgagacttgg gactttgaga cctgagactt gagacctgag tcttgcgagt
     2161 gttatattcc aacttggaac ttcacaaaca attctaatta acctgtgcag ccccgtggct
     2221 gcgattctgg aggtaatccc ttatgggaag cgacgactat cgacaagatc gaggtgacag
     2281 cgtctccacc cgacttactt atctactaat tggtggcggt atcggagcgg tgatcgctct
     2341 gctgtttgcc cctaaatccg gtcaggaact gcgcgaagac attgctgatg ctactcggaa
     2401 aggcattgac cgttcacgcg aagctgccca acaactcggc gagcgagccg gcgagtatta
     2461 cgagacaggc cgggaacgcg cggcagaact ctattcaact gcttccgagc gtgtgggcga
     2521 tgcggcccgc acggctcgtg acactgcgtc cgatctcgcg cgcaccgcac gcgaaactgc
     2581 ggcgcgccag acgggaactg tggcggcagc tatcgatgcc ggcaagaaag cctatcagga
     2641 agaaaagcgc aaaaccgaaa tgtctggtcg catcgaggct gctcctaagt acgacgagaa
     2701 agcgaactaa ggaattgccg attggcgatt gccgattgac gattccagga aatcggcaat
     2761 tggcaattgg caatcggaaa tgtgcggtta cggaccgaac aacaacgccc catgacgtta
     2821 gcgcttcaat tcaacaccca agaccccatg ttctgggtaa tggtggttat tgccctgtcg
     2881 tttatcatca tggcgatcgc gatggtttcg atcgccgtgt ttgtcagccg tgcggtcaaa
     2941 tcggtgaacc ggttggaaga aaagctcgag ccgctgatcg agaaggttac tgttgtcagt
     3001 gaacagggaa aacagatagc gatccagggc aagcacattg ccgaacaaat caccgcggtc
     3061 tcaagtcatc tttccactgc gacgatgcat ttctcggagt cgctggcaat tctgaaagac
     3121 gaagttggcg agttgaaaga agtcgtcagt catggcgcac gcgaagcgcg cgacaaagtc
     3181 gagctcgtca gcaacactat cgaccgcacg caccggcaag tcacaacgac gacggatttc
     3241 attcagtcaa aagtgattga gccggcgcgc gagctggccg cgattatggc gggcttccgc
     3301 cgcggcctcg aagtactcgt ggctcccgcg ccgaagccca ttaatcaaat ctacggagag
     3361 gatgagatgt tcatcggctg aattcggtct ccatcgtgca atcgaacccg aaccatcgcg
     3421 tcctccggga gctggttcgg gtttttactt gcccgtagtt ctagctcgct cacttccagc
     3481 atctgagatc tgacatctca aatttgagat ctcagtgtta gtattaatga ttcttacgcc
     3541 ctgaaagctt atgtccaccg aaacccttga tctgaaaaag acagtcaatc ttcccaaaac
     3601 cggcttcgct cagaaggcga acctggcgca gaacgaaccg gcccgcctga aaaaatggtc
     3661 ggaactaaag ctctacgacc tgattcgtca ggatcgcgcc tgcgccgaaa agtttgtcct
     3721 ccatgacgga ccgccttacg ccaacgccga catccacttg ggcaccgccc tgaataagat
     3781 tctgaaagac ttcatcgtca agtcgcgcac gatgatgggc ttcgacgccc catacgttcc
     3841 cggttacgac tgccacggtc tgccgattga gctttacgtc gatcgcaagc tcggcgcgaa
     3901 gaaagccaac atgcccccgg tttccatccg gcgcgcctgt cgcgagcatg ctgcggaagc
     3961 actgaaacga cagacacgcg actttcagcg gctggctatc ttcggcgagt gggagaatcc
     4021 ttacctcacc atgtcgaatc attacgaggc ggagactgcg cgcctgttcg gtcgctttgt
     4081 agagcgcggt tacgtttaca aaggcgcgcg ccccgtctac tggtgcattc acgatcaaac
     4141 cgcactcgcc gaagcggagg ttgaatacca tcaacactcc agtccctcgg tgtatgtcaa
     4201 attccccttg cgcagcgatc cggcactgat cgatccagcg ctcgccggac gcaaagtctt
     4261 tgttttgatc tggacgacca cgccctggac cctcccggca aacctgggaa tcgcagtcca
     4321 cccagacttc gagtactccg cgtttgcaca tggcgatgag gtttttatcg tcgccagtga
     4381 gctgctggag tcggttgccg agaaatgtgg actgaccgcc actggcgccg aaccacttca
     4441 agtgctggct cgctttcccg gtgccaggct tgaccgtctc gagtgtggcc acgcgtggct
     4501 ggatcgcgct tcactgttaa tggtgggtga tcacgtaacg ctcggaggcg aagccgatgc
     4561 ggaggtggaa ctcgatgtcc gcgaggcacg cgatccgaag aagactggca aatcgggaac
     4621 cggctgcgtg catacggccc ctggtcacgg gcacgacgac ttcgtgctcg gtaagagcta
     4681 cggcctggaa atctattgtc ccgtggataa ctccggacac ttcactcccg aagttgaaca
     4741 tttcgcaggt cagagcgtgt ttgcagcgaa cccgaaaatt gtcgagttca tgcgtgaacg
     4801 cggcgtcttg ctctttaccg aaacctacga acaccgctat ccgcactgct ggcgctgcaa
     4861 gaatccagtg atctttcgcg ctacgccgca atggttcatc tccatggatc gggcgggtgg
     4921 gccggtggta gaacgcgacg aagacggccg tgaccgcagc aatttcaccg ataatttctc
     4981 tgacgcgagt tcttcgggtg ctaattcgtt gcgcgtgact gcgcttgcgg aaatcgagcg
     5041 cgtgcaatgg ctgcccgcct ggggcttgga tcgcatgcgc aacatgttca aaggccggcc
     5101 ggactggtgc gtttcccggc agcgcgtctg gggcgtttca attcctgttt tttattgtgc
     5161 aaagtgtaat gaagcggtcg ctgatccgca gatcattaat cgggttgccg acgtgtttga
     5221 gaaagagtcg gcggatgcct ggtacacgcg caccgcaagc gagctgctac cagaaggcta
     5281 taagtgcggc aactgcgggg agcaggactg gatcaaggag actgacattc tcgatgtctg
     5341 gttcgacagc ggctcgtctt caatcgctgt gttggagcac cggcccgagc tgcgctggcc
     5401 cgcagacgtt tacatcgagg gcgg
//
LOCUS       DASUEX010000232         2252 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_619780, whole genome shotgun sequence.
ACCESSION   DASUEX010000232 DASUEX010000000
VERSION     DASUEX010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_619780"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..593)
                     /locus_tag="VFV61_03915"
     CDS             complement(<1..593)
                     /locus_tag="VFV61_03915"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: protein motif:HMM:NF014498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase"
                     /protein_id="HEX4899651.1"
                     /translation="MINETTTSGMYEKMKAILSDTVPDFELRIKAEVAAEINRLKAEK
                     NAVILGHNYMEPALFNSIPDYVGDSLMLARKAAETQKDIIVFCGVRFMAETAKILNPT
                     KKVLLPSAKAGCSLAASITAADVRELKRKFPGVPVVTYINTYADVKAESDICCTSSNA
                     VAVVESLKSDTVIFIPDEYLAGNVARETGRHIIFPTR"
     gene            complement(690..1505)
                     /gene="nadC"
                     /locus_tag="VFV61_03920"
     CDS             complement(690..1505)
                     /gene="nadC"
                     /locus_tag="VFV61_03920"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEX4899652.1"
                     /translation="MSLIKRALAEDIGEGDATTNSIVPVEAVLKGEIIAKQDGVIAGL
                     DVAREVFFSLNPSIRFDTATTEGSQVTKGTALAHVSGSARALLTGERTALNFLGRMSG
                     IATLTRQFVNAVAGTAATILDTRKTAPGLRLIDKMAVRRGGGENHRQGLFDMILIKDN
                     HIDFAGSLAEAVNRVRATGTQLEIEVETRTIEDVQQALALGVNRILLDNMSPATMREA
                     VVLNAGRARLEASGNVTLENIREVAETGVNYISVGALTHSAKVFDVSLNWIGR"
     gene            complement(1663..2070)
                     /locus_tag="VFV61_03925"
     CDS             complement(1663..2070)
                     /locus_tag="VFV61_03925"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX4899653.1"
                     /translation="MISDEGQPNTGARAEQELRQVNEEWAKALAQRDRAALERIIADD
                     FVLAYPFDGDDKSQFIDYVIRGDLTIGALESREENLRVLDQIGVVFGSETAHWLYRGR
                     DLTGPYRFVRVYTWRDGRWQMIILHVCSLAHHQ"
BASE COUNT          488 a          620 c          590 g          554 t
ORIGIN      
        1 tcgcgggtcg gaaagatgat gtgccgtcct gtttcgcgcg cgacgttgcc ggcgaggtat
       61 tcgtccggaa taaagatcac cgtatcgctc ttcagcgact caaccaccgc gactgcgttt
      121 gacgacgtgc aacagatgtc tgattcggct ttcacatcag cgtaggtatt gatgtaagta
      181 acgaccggca caccgggaaa tttccgcttc aactcgcgca cgtccgcggc agtgatactg
      241 gcagccaggg agcagcccgc tttcgctgac ggcaagagca ctttcttggt agggtttaga
      301 atcttcgccg tttcagccat aaacctgacg ccgcaaaaga caattatgtc tttctgggtc
      361 tcggccgcct tgcgggcaag cattagcgag tcgccgacgt aatcaggtat cgaattaaaa
      421 agggcaggct ccatgtagtt gtgccccagg attacggcat tcttctccgc ttttagccga
      481 ttgatctccg cagccacttc cgccttaatc cgcagctcga aatcgggcac agtgtcagac
      541 agaattgcct tcatcttttc atacatgccg ctggttgtgg tttcgttaat cattccatcc
      601 tcagtgatca agcttgtaac ccaatatctc agttctcaaa ttggatccag tctacgctgg
      661 ttattagttg atcaattcgt gttcagagtt taccgcccga tccagttgag actcacgtca
      721 aagactttcg cagagtgggt cagcgcccca accgaaatat aattgacacc cgtttccgca
      781 acttcgcgta tgttctcaag cgtaacattt cccgaggctt ccagtctggc ccgcccggca
      841 ttcaggacga ccgcttcgcg cattgtcgca ggagacatgt tatccagcag gatacgattg
      901 acgccgagcg ctaaagcctg ctgcacgtcc tcgattgttc gagtttccac ctcgatctct
      961 agctgagttc cggttgcgcg cactcgattc actgcctcag ccagcgaacc ggcaaagtcg
     1021 atgtggttgt cctttattag aatcatatca aacagcccct ggcgatggtt ctccccgcca
     1081 ccccggcgca cagccatctt gtcgatcaac ctaagacctg gcgcagtctt gcgcgtatcg
     1141 agaatagttg ctgcggtccc ggccacagca tttacaaatt ggcgcgtcag tgtggcgatg
     1201 cctgacatgc ggccgagaaa attgagggcg gtgcgctcgc cggtcaagag cgcgcgggca
     1261 gaaccactga cgtgggccag cgccgtccct ttagtaacct gtgatccctc agtcgtcgca
     1321 gtatcgaagc ggatgcttgg gttaagcgag aagaaaacct ccctggccac atccagtccc
     1381 gcgatgacgc cgtcctgctt ggcgatgatc tcgcctttga gcacggcttc aacgggcacg
     1441 atgctattgg tcgtcgcatc accctcgcca atgtcctctg ccagcgcgcg tttgattaag
     1501 gacatgattt caggatgcag ggttgaattc attttcgctt aagaatttcc caggtacgta
     1561 gtttggcgcc gaagttgtgt tgccggttga aagggtaggt taacgcacgg cgcactaatt
     1621 ttgctaagcg gggcggtagg tatccatcac agtgaaagtg cctcactggt gatgagccag
     1681 cgagcagacg tggaggatta tcatttgcca gcggccgtcg cgccaggtat agacgcgcac
     1741 aaatctgtaa gggccggtca ggtctcggcc tcggtagagc cagtgggcgg tctcactacc
     1801 aaaaactaca ccaatctgat cgaggacccg caaattctcc tcacgtgatt cgagagcgcc
     1861 gatagtcagg tcgccgcgga tcacgtagtc gatgaactga cttttatcgt cgccatcaaa
     1921 tggataggcg agaacaaaat cgtcggcaat gatgcgttca agcgctgccc ggtcgcgctg
     1981 ggccaaagcc ttcgcccatt cttcgttcac ctgccgtagt tcctgttcgg ctcgggcacc
     2041 cgtatttggc tgtccttcat cgctaatcat tgctcgcctc cgaggggatt gtggcgaaag
     2101 cttttatctt aacttgaagc ccaaagcaat agatgttcgg tccactcaga tcaaaagtgt
     2161 gtcaagtgac ggtaaatcga atccaataaa ttgcaggcag gtcaatcctt tcgttgtttt
     2221 agtgtgattt cgtggatcgt tttcgtactg ga
//
LOCUS       DASUEX010000233         2200 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1536916, whole genome shotgun sequence.
ACCESSION   DASUEX010000233 DASUEX010000000
VERSION     DASUEX010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1536916"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="VFV61_03930"
     CDS             complement(<1..250)
                     /locus_tag="VFV61_03930"
                     /inference="COORDINATES: protein motif:HMM:NF045191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899654.1"
                     /translation="MGYFKDLREHIAALDQAGLLVKIDEQINKDTELMPLVRWQFRGL
                     SEEERRAFMFTNVVDAKGRKYDIPVVVGTLAASRRIYGF"
     gene            complement(651..1691)
                     /locus_tag="VFV61_03935"
     CDS             complement(651..1691)
                     /locus_tag="VFV61_03935"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEX4899655.1"
                     /translation="MEYIALDVHKKYTWARVENAKGDRLYESRLAHAHGTVKNFVNRW
                     SAGSPVAVETVGNWYWVVDEIEAGGGKPQLVNARLAKLMMGSVNKSDKLDAKGMNRLQ
                     RTGTLPTVWIPSSSVRDARELPRTRMVLSRQRTQLKNRVHATLGKYGYTVEGASDAFG
                     KKGRKIIEEVLLKLPPHTQQALKLILDELDHVTDNLQAIEGQMIEMFAPCPQTNWLKS
                     LPGVGNILTVVIWTEIGRIERFGRAEQLASYCGLVARENSSGGKIRFGAVRRDVNVYL
                     KWAFVEAANSALLNAERCGYGHIHRLYQRIRPRRGHGKAKVAVARHLSEASFWMLKKG
                     EPYREPLSSTQR"
     gene            complement(1822..>2200)
                     /locus_tag="VFV61_03940"
     CDS             complement(1822..>2200)
                     /locus_tag="VFV61_03940"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX4899656.1"
                     /translation="VRVGLAPDSGGSFILPRLVGVSKAMELLLLGDTVDAKEAQRIGL
                     VARIFPASEFAASARALAEQVARAPRGIGLIKRAISHANWPNLENDLEYEAHLQEIAG
                     RSADYDEGVKAFLEKRTPVFTGK"
BASE COUNT          445 a          672 c          553 g          530 t
ORIGIN      
        1 caaacccgta aattcgtcgt gaagccgcaa gcgtaccaac gacgacggga atgtcatact
       61 tgcgaccctt ggcgtcgacc acattcgtga acataaacgc gcgtcgctcc tcttcggaaa
      121 ggccacggaa ttgccaccgc accaacggca tcagctcggt gtctttattg atctgctcat
      181 cgatcttgac cagcaatccc gcttgatcga gcgcggcgat atgttctctc agatccttga
      241 aataacccat gtctgatccc ccgaaacgta atctctagcc gtgatatttg atttcgctcg
      301 cgaaagccag cagcgagaga agtgctaacg aggatggaaa gccgcttaca agtctgtcac
      361 cctgagcgga gcgaagggtc tgctttctcc gctttcctcg gactagccta acctaggcga
      421 tgcagattct tcgtcgttgc actcctcatc tatgaaagct ttgtcaaggt ggctgtagta
      481 tcaaaaaggt atttcctcag atgtttttga gtaaacctct cctcgttgtc cgccattcac
      541 acccgatgtt atcggactca tctattcgcc gtcttatgcg gcagagaaat atttcacaac
      601 gctatcgcaa tcagaatgcg ggtctcagtg gacccatgag gcgcacgcgc ttagcgttgc
      661 gtggacgaca acggttcccg atagggctct cctttcttaa gcatccagaa actggcttcc
      721 gagaggtggc gcgccacggc tactttggct ttaccatggc cgcgccgggg cctgatgcgt
      781 tggtaaagtc gatggatgtg accatagccg cagcgctcag cattaagcag cgcactattg
      841 gccgcttcga caaaggccca tttcaaatac acattgacat cgcggcgtac cgcgccaaag
      901 cgaatcttcc ctccgctgga gttctcccgc gccaccagcc cgcagtaact cgccaattgc
      961 tcggcccggc cgaaccgttc aattcttccg atctcggtcc aaatcaccac cgtcaggata
     1021 ttgcctaccc caggcaaact cttaagccag ttggtctggg gacagggagc aaacatctcg
     1081 atcatttggc cttcgatcgc ttggagattg tccgtgacgt gatcgagctc atcgaggatg
     1141 agcttaagcg cctgctgggt gtggggtggg agcttaagca gaacttcctc tatgatcttg
     1201 cggccctttt tgccaaaggc gtcgctcgcc ccctccaccg tatagccata cttgcccaaa
     1261 gtggcatgca cgcggttttt cagttgtgtt ctctggcggc tgagcaccat gcgcgtgcgc
     1321 ggcagctcgc gtgcatcgcg caccgaagag gaagggatcc ataccgtggg cagggttcct
     1381 gtcctctgca gccggttcat tcctttagcg tcgagcttgt cgctcttatt gacgctgccc
     1441 atcatcagtt tagccaatcg cgcgttgacc agttgcggct tcccacctcc ggcctcgatt
     1501 tcatcgacta cccagtacca gttgccaacg gtctctaccg ccaccggaga tccggcgctc
     1561 catcgattga cgaaattctt gaccgtccca tgagcgtgcg ccagccgact ctcgtagagt
     1621 cgatctccct ttgcattctc tactcgtgcc caagtgtatt tcttgtgaac gtccaatgcg
     1681 atgtattcca taacggctgc cctcctcgac cgctcatctt atctacagcc gttacactat
     1741 tcatcccttt catattaatc agaatgacat tctgaatgaa ccccattttt cgacaccgtg
     1801 atagtctctc gagccgggtc cctattttcc ggtaaacacc ggcgttcttt tttccaaaaa
     1861 ggcttttacg ccttcgtcgt agtccgcgct ccgcccggcg atttcctgca agtgcgcctc
     1921 atattcgaga tcgttttcca gattcggcca gttcgcgtga ctgatcgcgc gtttgatcag
     1981 gccgatgccg cgcggcgcgc gcgcgacttg ctcggcaagg gcgcgcgcgg acgcggcaaa
     2041 ctcgctggct ggaaaaatcc tcgctacgag accgatccgc tgcgcttctt tggcatccac
     2101 ggtatcgccg agcaggagca actccatcgc tttgctgacg cctacgagcc ggggcaggat
     2161 gaagctgccg ccggaatccg gggccaggcc gacgcgcacg
//
LOCUS       DASUEX010000234         4724 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1633923, whole genome shotgun sequence.
ACCESSION   DASUEX010000234 DASUEX010000000
VERSION     DASUEX010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4724
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1633923"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1083
                     /locus_tag="VFV61_03945"
     CDS             <1..1083
                     /locus_tag="VFV61_03945"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX4899657.1"
                     /translation="RVSLVTLCLSALIVVAEIVTPGFAQRVTPVNPTATQAKATPTPT
                     PPPTQAEQEVDPDDVISVETTEILLPVTVRDANGELVPGLTRTDFRVFEDGVNQPLSD
                     FSLRKVPVDAVLMVDASSSVSSNLEDFRAAAEGFASHLAVADRISLIQFDDRVLLLQD
                     WTKSLVQLRRALRRLSPGMFTRFNDALLLAARDQMPRGNARHAIIVLTDGIDSGRGTT
                     FEAALRAALESQTTVYVVSNSEIERAKKQAELSGLESGSDASLRFNEIKMNDLRLGLE
                     ALTASERNMAQLTSATGGRLYKPQSFSDLEKIYAEVADELRHQYALYYSPLNRKRDGG
                     FRRVKVETRSPALQVSARIGYFAPKK"
     gene            1451..3496
                     /locus_tag="VFV61_03950"
     CDS             1451..3496
                     /locus_tag="VFV61_03950"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX4899658.1"
                     /translation="MRPVKHAEKGLTLIAGALHDVIQSVNKYKPNPSFTPKWSDKPLL
                     KSWQKSKPTLGWPRTTDSLCPNCVIEARESILSGKQDVSVLINEKVGEIKAQIIERDG
                     QVWMVKDCPIHGHFEDMMAIDVKFLTHIEKMFPGRDIDAHNDEKLHNHGSSTVKYGRG
                     AVLTVDLTNRCNMMCDPCFMDANQVGFVHELSMDDIKEILDNAITIKPRRQMSVQFSG
                     GEPSISPHFLESIRYARKVGYNSVQAATNGIEFAKSKEFCREAAAAGLRYVYLQFDGI
                     GNDANSHRQVGNLFDVKMRAINNLHEAGVEIVLVTTLVNGINNDQVGSIVRFALDNPK
                     KIAFLSFQPVSFTGRDEEITEQRRLQQRYTLSHLAHDVKNQVGITEPTRDWFPLSLMG
                     AFADFADLVHGPQAEWGQVSCGCHPNCGVGTAVMVDKETREMRPVPEFLNIPGLVKDM
                     QKITDAARGKWMSNMMMGLALLKHYNPYSSPSQFTLYELFKKFDKSFGLTGKDYGKVS
                     GDRTTQDIEVRRSDRWNFLFIAGMWFQDLFNYDFRRTEMCIIPYGTQEGEISFCAYNT
                     GIGWRNIIENMHQNATVAKWYEEHGRHEIFARGKEVELADKSHAMVLNPIDLARPNKP
                     EMEGPKTAHEEAVMMRKLYQELVLKQQLDTKEADKPVQIQGLGRKKAEPATAEVVAV"
     gene            3705..3914
                     /gene="rpsU"
                     /locus_tag="VFV61_03955"
     CDS             3705..3914
                     /gene="rpsU"
                     /locus_tag="VFV61_03955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEX4899659.1"
                     /translation="MAIVTVNQGESIESALRRFKRKVMTEEIIKDAKKHAFFIPPGQK
                     AKLKSALARKRNKKKGRMRSAPPRD"
     gene            3936..>4724
                     /gene="surE"
                     /locus_tag="VFV61_03960"
     CDS             3936..>4724
                     /gene="surE"
                     /locus_tag="VFV61_03960"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEX4899660.1"
                     /translation="MRRAAVAGTSQQFLLETSGRGELPTIHEGAASREAVNVKSPMTR
                     ILLTNDDGIHSDGLIKLEAALKEVGDVYVVAPESEMSGAAHSLTLARPLRIRQIDERH
                     WTVDGTPTDCVTLALNRILPAEELPHICASGINHGGNLGDDATYSGTVAGALEATILG
                     VPGLAFSLVARDNFDFSEPARFAITAVRKVLTEGLPEGTLLNINFPRGEIKGVRVTRQ
                     GIKNARPVISEHIDPRGKPYFWIGEEYFNKGAAEGTDYQAIEQGC"
BASE COUNT         1169 a         1247 c         1301 g         1007 t
ORIGIN      
        1 cgcgtttcac ttgtcactct ttgcttgagc gcgctcatcg ttgttgctga aatcgtcaca
       61 cccggatttg ctcaacgagt aacgccagta aatccgacgg ccactcaagc gaaagccacc
      121 ccaaccccga cacctccacc aactcaagca gaacaggaag tcgatccaga cgatgtgatt
      181 tccgtcgaga cgacggagat tctgttgccg gtcacggttc gcgacgccaa tggggaattg
      241 gttcctggtc tgacgcgaac agattttcgc gtcttcgaag acggagtcaa ccagccgtta
      301 agcgacttta gcttacgcaa agtgcccgtg gatgcagttc taatggttga tgcttcatcg
      361 tctgtttcaa gcaatcttga agactttcgc gcggccgccg aaggcttcgc ctcgcacctg
      421 gcggttgctg accgcataag tctgattcag tttgacgatc gagtgctctt attgcaggac
      481 tggacgaaga gtctggtgca gctccgtcgg gctttgcgcc gattgtcacc aggtatgttt
      541 acaagattca acgacgcgtt gctgttggca gcacgagatc agatgccccg gggaaacgcc
      601 cgccacgcga tcatcgttct aactgatggc atcgacagtg gacgcgggac cacatttgaa
      661 gctgcgttac gggctgcgct tgagtcgcaa accaccgttt acgtcgtcag caactcagag
      721 attgagcgag caaagaagca agcggagctc tccggattgg aatccggttc agacgcgtca
      781 ctccggttca atgagattaa gatgaacgac ctgcggctcg gtctcgaggc actgactgcg
      841 agcgagcgaa atatggcgca actgaccagc gccaccggcg gtcgccttta caagcctcaa
      901 tcctttagtg atctggaaaa gatatacgca gaagtggctg acgaactgcg acatcagtat
      961 gctctctact actcaccgct aaaccgaaag cgcgacggtg ggttccggcg cgtgaaggtg
     1021 gaaactcgca gcccagcgct tcaggtatct gctcgcattg gctacttcgc gccaaagaaa
     1081 taggaacctg cactccagcg caaaaccttt cctaaatgaa gatgatcggc gaagatattt
     1141 cagtttttgt cactagctca ccaagaccgg ataaaaggta acgctcccaa atcagtgaga
     1201 gccagaatgg ctcacatggc agtttcgcag gcgatgtgtg cgctcacgac acatctcgga
     1261 ccatgcgaga tcaggagatt tcgtgtcctg gtttaggggt aaacctcaat atttcagcag
     1321 ttacgtccag gtgagcgtgg gcggcatggt cattgccaga tgcttcgtgc tagtcttgca
     1381 gccgttttgt taagtacccc cgggctagcg agcaatcaat aaaattcacg aagaaggaag
     1441 acgagattcg atgcgaccag tcaaacacgc ggaaaagggc ctaaccttaa ttgccggagc
     1501 tcttcacgac gttattcaat cggtaaataa gtacaagcca aatccctcct ttacgcccaa
     1561 atggtcagac aagccgctgc tgaaatcctg gcaaaagagc aaaccaacac tcggttggcc
     1621 gcgaaccacg gattcgcttt gtccgaactg cgtaattgaa gcccgcgagt caattctcag
     1681 cggcaagcag gatgtcagcg tcttaatcaa tgagaaggtc ggcgaaatca aggcccagat
     1741 cattgaacgc gacggtcaag tctggatggt gaaagactgc ccaatccacg ggcatttcga
     1801 agacatgatg gccatcgacg tcaaatttct gacgcacatc gaaaagatgt ttcccggtcg
     1861 cgacatcgat gcccataacg acgaaaagct gcacaaccat ggatcaagca cggtgaagta
     1921 cggccgcggt gcggtgctga cagtggatct gactaaccgc tgtaacatga tgtgtgatcc
     1981 ctgcttcatg gacgcaaacc aggttggttt cgtgcacgag ctgagcatgg acgacatcaa
     2041 agagattttg gacaacgcta tcaccatcaa gccgcgccgg cagatgtccg tgcagttttc
     2101 cggtggtgag ccatcaattt cgccacactt cctcgagtcg attcgctatg cacgcaaagt
     2161 tggatacaac agcgtgcagg cggcaactaa tggaattgag tttgccaaga gcaaagagtt
     2221 ctgccgggaa gcagccgcag cgggactgcg ttatgtgtac ctccagttcg atggtatcgg
     2281 taacgatgcc aacagccacc gccaggtggg caacctcttc gatgtgaaga tgcgcgccat
     2341 caacaacctg cacgaagcag gagttgaaat tgtgctggtg acgacgctgg ttaatggaat
     2401 caataacgat caggttggct cgatcgtgcg tttcgctttg gacaacccca agaagattgc
     2461 ttttctgtcg ttccaaccgg tttcgtttac tggccgtgac gaagagatta cggagcagcg
     2521 ccgcttgcag cagcgctaca cgctgtcgca tctggcgcac gacgtgaaaa accaggtggg
     2581 tatcactgag ccgacacgcg actggtttcc cctgtccctg atgggagcat tcgcagattt
     2641 tgctgatttg gttcacggcc cgcaggccga gtggggtcag gtttcgtgcg gttgtcatcc
     2701 taactgcggt gtgggcacgg cggttatggt tgacaaggaa acccgcgaga tgagaccagt
     2761 gccggaattc ttaaacatcc ctggcctggt aaaagacatg cagaagatca ctgacgcggc
     2821 ccgcggcaag tggatgtcga acatgatgat gggactggcg ctcttgaaac actacaatcc
     2881 atacagctcg ccctcccagt tcactctgta tgagttgttc aagaagttcg acaaatcgtt
     2941 cggtttgacg ggcaaggatt acggcaaggt cagcggtgac cgcaccacgc aggatatcga
     3001 ggttcgtcgt tcggatcgtt ggaacttcct ctttatcgcc ggcatgtggt tccaggatct
     3061 cttcaactac gacttccgcc gcaccgagat gtgcatcatt ccctacggca cgcaagaagg
     3121 tgagatttcc ttctgtgctt acaacacggg aattggctgg agaaatatca tcgagaacat
     3181 gcaccagaat gcgaccgtgg cgaagtggta tgaagagcac ggccgtcacg agatttttgc
     3241 ccgcggcaag gaagtcgaac tcgcggacaa gagccacgcc atggtgctca atccaattga
     3301 cctggcgcgc ccgaacaagc cggaaatgga aggtccgaag actgcgcacg aagaagctgt
     3361 gatgatgcgc aagctttatc aggagctggt gctcaagcag cagcttgata cgaaggaagc
     3421 ggacaagccg gtccagattc agggactcgg ccgcaagaag gctgaacctg caaccgcaga
     3481 agtagtcgcg gtatagccct cgatctcgct ttagaaaact gtcggggcgt cctctttgga
     3541 cgccccattt cagcgtagag aggacgctcg ccagcgttgc cgctcatgaa ttttgcggcc
     3601 aaccgcaaag ttgccaactt gaccgcaggc attgctgacg tgttaaataa aggcgctgaa
     3661 taggctttac cactagaaag ttaattaaga gagacggaga tagaatggcg atagttactg
     3721 ttaaccaggg tgaatcgatc gagagcgcat tgcggcgctt caagcgcaaa gtaatgactg
     3781 aagagattat caaggacgct aagaagcatg cgttctttat cccgcctggc caaaaagcca
     3841 agttgaaatc ggcactcgca cgcaagcgca acaagaagaa aggtcgcatg cgcagcgccc
     3901 ctcctcgtga ctaacgagat ctttgccacc tctttttgcg acgcgcagca gtcgcaggta
     3961 cctcccagca gttcctcctt gaaacgagcg gacgaggtga actccccacg atccacgagg
     4021 gcgcagcctc gcgtgaagct gtgaacgtta aatcaccgat gactcgtatc ctgcttacta
     4081 atgacgatgg gatccactct gacggcttga tcaagctcga agcggctttg aaggaagtcg
     4141 gcgacgttta cgtcgtggct ccggagtcag agatgagtgg tgctgcccac agcctgacgc
     4201 tcgcccggcc actgcgcatt cggcagatcg atgagcgaca ctggacggtc gacggcacac
     4261 cgaccgactg cgtcacgctg gccctgaatc gcattttgcc tgccgaagag cttccccaca
     4321 tctgtgcctc cggaatcaat cacggcggca atcttggcga cgacgctact tattcgggta
     4381 cagtcgcggg tgcactcgaa gctaccattc tgggcgtacc gggactcgca tttagtttag
     4441 ttgctcgcga caacttcgac ttctctgaac cggcacgttt tgccatcacc gcagtacgta
     4501 aagtgttaac tgaaggattg ccggaaggaa cgttgttgaa tataaacttt ccgcgcggcg
     4561 agattaaagg agtgcgggtg acccggcagg gaatcaagaa tgctcgaccg gtaatcagtg
     4621 aacacatcga tccgcgtggc aaaccgtatt tctggatcgg tgaagagtac ttcaacaagg
     4681 gcgcggccga gggaactgac tatcaggcaa tcgagcaggg ctgt
//
LOCUS       DASUEX010000235         4651 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_262428, whole genome shotgun sequence.
ACCESSION   DASUEX010000235 DASUEX010000000
VERSION     DASUEX010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4651
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262428"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /gene="coaBC"
                     /locus_tag="VFV61_03965"
     CDS             complement(<1..663)
                     /gene="coaBC"
                     /locus_tag="VFV61_03965"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEX4899661.1"
                     /translation="MADDTLKTSFSGKRVALGISGGIAAYKAAEVLRGLQRAGCIVRV
                     AMSQRASEFITPLTFRALSGSHVIVDDYAPDNPDPIAHITFSQTVDLFVIAPATANII
                     AKFANGVADDFLTSTYLAATAPVLVAPAMNTSMWEHPATRRNIERLLQDGVHVIEPDA
                     GEMACGTIGPGRLSEPERIVAAALEILGQSKSPRQDLAGERLLITTGATREELDPIRF
                     ISN"
     gene            803..1360
                     /locus_tag="VFV61_03970"
     CDS             803..1360
                     /locus_tag="VFV61_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899662.1"
                     /translation="MRSLIPSFPLRLLGYAGFLPLAFLLAVTPCAAQDEQFGPVVRAY
                     LGYLRNEQEVVDDRASRREVSPAYYRHNSNRIRALRQIAIKIARETQNDYLPELEAVS
                     RDELRLLFEQPPRIETLKVNEVFGYTLRFLGSVRSGETFYVFARLDPYEQAELREKGA
                     KSANPRDEQGRGVKVSTSSPLATPP"
     gene            1681..2370
                     /locus_tag="VFV61_03975"
     CDS             1681..2370
                     /locus_tag="VFV61_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899663.1"
                     /translation="MISKSLSRKTLAAFVSVAVLSVYSMVALAGAKVASAELSVSGQV
                     TVNGQKVISGGTLFSGSTVVTADSSTAIVSLSKMGRVELSPNSSLILNFTEESVTGML
                     DKGVAKVSTVAGVSVNITTKDGAVVVDGSQTTSFTVSTNRGNTVVSTEAGLAELRSGA
                     ATQQIAAGENGTAGTPNPQTDDDDDDMSGGALAVLLLASAGAIIAVIIAAKEDNELQV
                     GGNAIVVSPSR"
     gene            2531..3829
                     /locus_tag="VFV61_03980"
     CDS             2531..3829
                     /locus_tag="VFV61_03980"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HEX4899664.1"
                     /translation="MHIAVIGTGYVGLVTGACFAEFGVDVTCVDVDAEKIERLKAGVM
                     PIYEPGLEHLVTKNAQAGRLRFTTNVQEAVEQALVIFLAVGTPPLSDGSPDLSFVESA
                     ASAVAEHMNGYKVIVTKSTVPIGTGERIRKLIREQQKSNLAFGIVSNPEFLREGAAIN
                     DFMRPDRVVIGSADQEAIAIMRDLYRPLYLIEAPVVITSLEAAELTKYAANAFLATKI
                     SFINEIANLCEKIGCDVHDVARAMGMDKRIGSKFLHPGPGFGGSCFPKDTRALASVAQ
                     QFGSDSLLVEAVIEVNRRQRSAMIPKIEKLVGAVKGKTIAVLGLAFKPETNDIREAPA
                     LDIIQGLLERGATVRAYDPVAMEETAKVISNICYAEDEYEAVKDADALVIVTEWNQFR
                     ALDMKRIRDLMKSPRIADLRNVYEPDDLRELGFEYSGVGR"
     gene            3857..>4651
                     /locus_tag="VFV61_03985"
     CDS             3857..>4651
                     /locus_tag="VFV61_03985"
                     /inference="COORDINATES: protein motif:HMM:NF014740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX4899665.1"
                     /translation="MGLSGIALVTGGAGFIGSHIAAALLANGARVRVIDDLSTGYREN
                     VQDAGADIDFVHASVADESALRKALEDVELVFHEAAIPSVPRSVENPRQTHIASVDST
                     FSLLLAAREMKVRRIVYAASSSAYGDQPTLPKVEDMLPVPLSPYAVAKLVGEYYCQVF
                     TRVYGLETVSLRYFNVFGPRQDPSSQYSGVISRFIAALLSGAQPVIFGNGEQSRDFTY
                     IANVVEANLKAAETTKGIGAVINVANGARITLNELLKEVQQLTGKTD"
BASE COUNT         1057 a         1193 c         1290 g         1111 t
ORIGIN      
        1 atttgaaatg aagcggatcg gatccaactc ttcacgggtc gcaccggtgg tgatcagcag
       61 ccgttcgccg gctagatcct gacgtggcga cttcgattga cctagaattt ccagagcagc
      121 cgccacaatc ctctcaggct cactcagtcg ccccgggcca atagtcccgc acgccatttc
      181 accggcatcc ggttctatta catgaacacc gtcctgtagt aatcgttcga tgttccgccg
      241 ggtcgccggg tgttcccaca ttgaggtgtt cattgccggc gccaccagaa ccggggcagt
      301 ggcggccaga taggtggatg ttagaaaatc atctgccacg ccattagcaa atttggcgat
      361 gatgttcgca gtggcgggtg caataacaaa aaggtcgact gtttgggaaa atgtaatgtg
      421 ggctattgga tcgggattgt ctggtgcata atcatccacg atcacatgcg aaccagagag
      481 tgctcgaaag gtgagtggag taatgaactc agaggctcgt tgtgacatgg ccacgcgcac
      541 gatacagccc gcacgctgca gtccgcgcaa cacctcggcg gccttgtagg cagcaatccc
      601 cccgctgatt ccgagggcga ctctcttgcc actgaaactg gtttttaggg tgtcgtcagc
      661 catccggagt ggttcttact ggagaaatga tgctcaatgt aacattgctg aaacacctgc
      721 gcaatcaaag caaaaacagg tacttgttac cattaggcac cctcgactcg ttaattagca
      781 gtttccgcag tttgattctc taatgaggtc attaatccct tcattcccac tacggttgct
      841 cggctacgca ggttttctgc cgctcgcgtt tttgctcgcc gttacgccct gcgctgccca
      901 ggacgagcag tttggtcctg tcgtgcgggc ctatctcggt tatttgcgta atgaacagga
      961 agtggtcgac gaccgcgcca gtcgccgcga ggtgagcccc gcctattatc gacataattc
     1021 gaaccgcatc cgggccctgc ggcagatagc catcaagatt gcccgcgaaa cccaaaacga
     1081 ttatttgcct gaactcgaag cagtttctcg ggatgagttg cgacttttgt ttgaacagcc
     1141 cccgcggata gaaacgctga aggtaaacga ggtgtttggc tacactcttc gctttctggg
     1201 aagtgttcgc agcggtgaaa ccttctacgt ttttgcacgg cttgatcctt acgagcaggc
     1261 agaattgcga gagaaggggg ctaaatccgc caatcctcgt gacgagcagg gtcgtggcgt
     1321 gaaggtctcg accagttccc ctctggcgac accgccgtga cggtattaac accgatcgag
     1381 gttgtattct tccattacgc atgcgaaaag gatggactgg tcctacctca ggtgatatct
     1441 tgtcaacaac cgcaaaaaag cacagttttg tggcaaagtc ggactaaatg atcgttgaca
     1501 gggggcttat attcgtgtaa taaaaagcct gctgttaagc gcctgcgaac gtgatttgcg
     1561 cttgtagcgt tcggccgagt gcatctttga gacggggatt gactagacgt tcgactcatc
     1621 ctggtttctt actagcgcca gcaatcaaga ttcttgtttc acaatacggg aggaaagtta
     1681 atgatcagca aatctttgag ccgcaaaacg ctcgcggcct ttgtctcagt tgcagtcttg
     1741 agtgtttact cgatggttgc actggcaggt gcgaaggttg cgtccgcaga gctttcagtt
     1801 tccggccaag tcacggtgaa cggtcagaaa gttatttctg gcggaactct gttttcaggc
     1861 agcacagtag taaccgctga cagcagcacc gcaatcgtga gcttgagcaa gatgggccgc
     1921 gttgagttgt ctccaaactc cagcctgatt ctcaatttca ccgaagagag cgtcaccggt
     1981 atgttggata aaggtgtcgc caaagtctca acggtcgcag gggtctcggt caacatcacc
     2041 acgaaagacg gcgcggtggt tgtagatggt agccagacaa catcgttcac agtcagcacc
     2101 aatcgcggca acactgttgt ctccactgag gccggtctcg ccgagttgcg ctcgggcgca
     2161 gctacccagc aaatcgctgc tggtgaaaat ggcaccgccg gaactccgaa tccacagaca
     2221 gacgatgatg atgacgatat gagcggcggt gccctggcag tgctcctgct cgcttcagcc
     2281 ggcgctatta ttgccgttat catcgccgcg aaagaagaca acgagctgca agtcggcggc
     2341 aatgcaatcg tagtcagccc ttccagataa acagtcgttg cttcggccac tagaaatact
     2401 gcggtgcgat tttccgctgc ggtcattaac tactaacctt gcgggtcacg cgactctcgg
     2461 tcgttgtgac ccgcaaagtt tgtttgagtc ggcccttagt ttcccgccca cagtttccta
     2521 aggtcttcaa atgcacattg cagttattgg tactggttat gttggcttgg tgaccggtgc
     2581 atgtttcgct gagtttggtg tcgacgtcac ctgcgttgat gttgatgccg agaaaataga
     2641 acggctgaaa gcgggcgtca tgcctatcta cgagccaggc cttgagcact tggtaactaa
     2701 gaacgcccag gccggacggc tcagattcac gaccaacgtt caggaagccg tcgagcaggc
     2761 ccttgtgatc ttcctggccg tgggaactcc ccccttgtcg gatggttccc cggatctgag
     2821 ttttgtcgag tctgcggcga gcgcagtcgc cgaacacatg aacggctaca aggtaatcgt
     2881 cacgaagtcg accgtaccga ttggaacggg cgagcgcatc aggaaactaa ttcgcgaaca
     2941 gcagaaatca aatctggcgt tcggcatagt ttcaaatcca gagtttttgc gtgaaggagc
     3001 ggcgattaac gacttcatgc ggcctgaccg cgttgtgatc ggcagcgctg accaggaggc
     3061 gattgctatc atgcgggatc tttaccgccc gctctatctg atcgaggctc cggtcgtgat
     3121 cacttccctg gaagcagcgg agttgaccaa gtacgcggcg aatgcctttc tcgcgacaaa
     3181 aatatccttc attaatgaaa ttgcgaacct ctgcgaaaag atcggttgtg acgttcacga
     3241 cgtcgccaga gcgatgggaa tggacaagcg tatcggcagc aagtttctgc atccgggtcc
     3301 ggggttcggc ggatcctgtt tcccgaaaga cacacgcgcg ttagcttcag tggcgcagca
     3361 gttcggttct gattctctac tggttgaggc ggtcattgag gtgaaccgca ggcaacgcag
     3421 tgccatgata ccgaagatcg aaaagctggt cggtgcggta aagggaaaaa caatcgcagt
     3481 gctgggcctc gcttttaaac cggagacgaa tgatattcgt gaagcgcctg ccctggacat
     3541 tatccaggga ctcttagagc gcggggcgac tgtgcgtgct tacgaccccg tggcaatgga
     3601 agagacagcc aaagtgattt ctaacatctg ctatgcggag gatgagtatg aagctgtgaa
     3661 ggacgcagat gcgctggtaa tcgtcacgga gtggaatcag tttcgcgctc tcgatatgaa
     3721 gcgaattcga gatttgatga agtccccgcg gatcgccgac ttgcgtaatg tctacgaacc
     3781 agatgatcta cgcgaactcg gctttgaata ttcaggcgtt ggtcgttaat cctcaataga
     3841 attcaaacaa acttctatgg gcctttcggg aattgcattg gtcaccggtg gcgccggttt
     3901 cattggttca catatcgcgg cagctctctt ggcaaatggc gccagggttc gcgtcatcga
     3961 cgacctttca acgggttatc gagaaaacgt tcaagacgcc ggcgctgaca tcgattttgt
     4021 gcacgcgagt gttgccgacg aaagcgcttt gcgcaaagcg ctggaagacg ttgaacttgt
     4081 ctttcatgaa gcagcgattc cttcggtgcc tcgctcggtt gagaatccgc gccagacaca
     4141 catcgcctct gttgattcta ctttttcctt gctcctcgct gcccgggaaa tgaaggtcag
     4201 acgtatcgtc tacgcggcct caagttcagc ttacggagat cagccaacac tgccgaaggt
     4261 cgaggacatg ttgcctgttc cgctttctcc ttatgcggtg gccaaactgg tgggtgagta
     4321 ttactgccag gtcttcactc gagtatatgg actggagaca gtctcactta ggtactttaa
     4381 tgtctttggt ccccggcagg atccgagctc gcaatactct ggagtgattt cgcggttcat
     4441 cgctgccttg ttgagtggcg cgcagccagt gatttttggc aatggggaac agtcgcggga
     4501 cttcacttac atcgccaatg tggtcgaagc caatttaaag gccgcagaaa cgacgaaggg
     4561 catcggtgca gtgatcaatg tggcgaacgg tgcgcgtatt acgctcaatg agctcttaaa
     4621 agaggtgcaa caactaacag gtaagacaga t
//
LOCUS       DASUEX010000236         2172 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1537025, whole genome shotgun sequence.
ACCESSION   DASUEX010000236 DASUEX010000000
VERSION     DASUEX010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1537025"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            79..1545
                     /locus_tag="VFV61_03990"
     CDS             79..1545
                     /locus_tag="VFV61_03990"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX4899666.1"
                     /translation="MCPEDNTALQADPTVIGESAADPLIGHTLDEKYSLEERLGVGGM
                     GTVYRARHLLIDRAVAVKVLHQRFVEDEAARTRFHREAKAAGRLQHPNAVTVTDFGQT
                     SDGYVYIVMELLVGRTLRDVLAKEAPLDPARTVSLMLQISAAVSAAHEAGIIHRDLKP
                     ANIFIVQRAEVPSMVKVLDFGIAKLFADSLDEDDPQTLTLFGAMIGTPRYMSPEQCDG
                     AELTPAADVYSLGVIFYEMLAGTVPFSGSTPLAIAMKHATEAPRSPREIVASIPEELE
                     KVVLHALEKRPEDRPANAAEFRRELLETAERLGLEHAAITSAPDLAALRDIGTESPSG
                     RLVIDISKLRENRAATTSAGELTVISEPQKPRVLNGPESSPVGEKRNFPRVAVPFGNR
                     RRNIRAGAVISLLVLAMVFGATFLAMRSNTSTPAETVVVASPSPTAEPSPSPSPVARR
                     TEQQKKPGNENSNNNNSKKDSKVGSILKKAGRILKKPF"
     gene            1577..>2172
                     /gene="kdsA"
                     /locus_tag="VFV61_03995"
     CDS             1577..>2172
                     /gene="kdsA"
                     /locus_tag="VFV61_03995"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF003543.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HEX4899667.1"
                     /translation="MPSNVKTNSFQVGNATFGDGRLTIIAGPCVIESRDHALMMAREC
                     ASRAREAGLDFVYKSSFDKANRSSIKSFRGLGMEAGLEVLRQVKEELGVPVLTDVHEV
                     SQVAPVAEVADVLQIPAFLSRQTDLVLEAARSGRAVNVKKGQFLAPSDARNIVDKARA
                     AGCEKIMLTERGVSFGYNNLVVDMRSFPIMHEFGVPVV"
BASE COUNT          504 a          577 c          616 g          475 t
ORIGIN      
        1 ttctgactcg ccctccccga acaaactttt atgcttcatt gtcccacatg cgggagacac
       61 tatgcgccgg gcttgctcgt gtgcccggag gacaacactg ccttgcaggc agacccgaca
      121 gtcattgggg aatcggctgc ggatccactg attggccaca ctcttgacga aaaatactct
      181 ttggaagaac ggctcggtgt cggcggaatg ggcacagttt atcgcgcccg ccatctgctg
      241 attgatcgag cagttgcagt gaaagtcctg catcagcgtt ttgtagagga tgaagcagcc
      301 cgaacaaggt tccatcgtga ggccaaggct gccggacgat tgcagcatcc caatgccgtc
      361 acagtaactg actttggtca gacttccgac ggctatgtct acatcgttat ggagttgctg
      421 gtgggtcgga cgctgcgaga cgtgctcgca aaagaagctc cacttgatcc agctcgtact
      481 gtctcgttga tgttacagat ctcggcagca gtctcggccg cacatgaagc ggggatcatt
      541 caccgcgatc taaagccggc aaacattttc attgttcaac gggcggaagt cccttcgatg
      601 gtaaaagtgc tcgactttgg aatcgcaaaa ctctttgccg actcgcttga cgaggacgac
      661 ccccagacac tgacactctt cggcgcaatg attgggacgc cacgctacat gtctcccgaa
      721 caatgcgacg gtgcagaact gactccagcg gcagatgtct acagcctcgg cgtgattttt
      781 tatgagatgc tggcgggcac tgtgcccttt agcggatcaa cacctctcgc gatcgcgatg
      841 aaacacgcaa ctgaggcccc caggagccca cgtgaaatcg tggcttcaat tcctgaggag
      901 ctggagaagg tggtcctgca cgcactggaa aagagacccg aggatcgtcc ggcaaatgct
      961 gctgaatttc gccgtgaatt gcttgaaaca gcagaacgtt tgggacttga acatgcagcg
     1021 atcaccagcg cgcctgacct tgctgcgttg cgagatatcg gtactgagtc gccgtcgggc
     1081 cgactagtaa tcgatatctc taaattacgt gagaatcgcg cggcgacgac cagcgccggc
     1141 gaacttacag tgatcagcga gccacagaag cctcgagtgt tgaatggacc agaatcttcc
     1201 cctgtaggtg aaaagcggaa tttcccgagg gtagcagtcc cgttcggcaa cagaaggaga
     1261 aatattaggg ccggggcggt tatctcactg ctggttttag cgatggtttt tggcgcgact
     1321 tttcttgcca tgcgctccaa tacctccacg ccggcagaaa ccgtcgttgt ggccagtcca
     1381 tctccgactg ccgaaccttc gccatcacct tcaccggtag ccaggcgaac tgagcaacag
     1441 aaaaagcctg gcaatgagaa cagcaacaac aataactcga agaaagactc gaaggtcggt
     1501 tcgattttga agaaggccgg gcgtatattg aaaaagccgt tctgatcaaa acgaccatca
     1561 atccaacaca cttgtgatgc ccagcaacgt aaagaccaac agtttccagg tggggaacgc
     1621 cacattcgga gacggccggc ttacgatcat tgccggaccg tgcgtgatcg agtcccggga
     1681 tcatgcgctg atgatggcgc gtgagtgcgc cagcagagcg cgcgaggcag gcctcgactt
     1741 tgtttacaag tcatctttcg acaaggcgaa tcgttcgtcg attaagagtt ttcgcggact
     1801 cggaatggag gctggactcg aagtacttcg gcaagtcaaa gaggagttgg gtgtgcccgt
     1861 gctcaccgat gtccatgagg tttcccaggt cgcgccggtt gccgaagttg cagatgtgct
     1921 tcagatcccc gcgtttcttt cacggcagac ggatctggtc ctggaagcgg cgcgttcagg
     1981 acgcgcggtc aatgtaaaga agggccagtt tctggcgccg tcggacgcac gcaacatcgt
     2041 cgataaggct cgggccgccg gctgtgaaaa gatcatgttg accgagcgtg gcgtcagttt
     2101 tggttacaac aatctcgtag tagatatgcg gtcgtttcct atcatgcacg agtttggagt
     2161 gccagtggta tt
//
LOCUS       DASUEX010000237         2895 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_849328, whole genome shotgun sequence.
ACCESSION   DASUEX010000237 DASUEX010000000
VERSION     DASUEX010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2895
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_849328"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(250..654)
                     /locus_tag="VFV61_04000"
     CDS             complement(250..654)
                     /locus_tag="VFV61_04000"
                     /inference="COORDINATES: protein motif:HMM:NF024979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX4899668.1"
                     /translation="MKRKPKTYTISAVAEQYSLHPQTLRLYEREGLLKPSRSEGNTRL
                     YTDSDLERLELILSLTRDLGVNLAGVEIIMNMREKMDAMQHEFERFFLYLQSHAEEFT
                     NFTEPPPEGTSDALVRVSRTAVLRRRTTVSRG"
     gene            complement(789..1949)
                     /gene="dnaJ"
                     /locus_tag="VFV61_04005"
     CDS             complement(789..1949)
                     /gene="dnaJ"
                     /locus_tag="VFV61_04005"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEX4899669.1"
                     /translation="MAKQDYYQTLGVKRDAKPEEIKKAYRRLARKYHPDVNPGDKASE
                     ERFKLTTEAHDVLSDPKKRAVYDRFGQYSDNLADAAARGATPGSSSGGASPGFDFTGF
                     DWGTSSGAGSGTSFKDIFSDLFGGGATKEREPPRPQPKRGADIEMPLALSFEEALTGL
                     TTNITVNRSEQCTRCNGAGDIGGPVMICPTCKGSGQVQRAGGRLRFAQECTDCEGTGR
                     RRSPCSLCKGKGTIPKTETVKVRIPAGVETGSRVRIPGKGEGGRMGAPAGDLHIITNV
                     GKHKHFTRKGDNIYVTVPITVPEAALGAKIEVPTVEGKAQLRIPPGTQSGQKFRLRER
                     GAPVLRNPGSKGDQFVEVQVILPKVISEETKELLRQYAKMNSDNPRVEMGLE"
     gene            complement(2078..>2895)
                     /locus_tag="VFV61_04010"
     CDS             complement(2078..>2895)
                     /locus_tag="VFV61_04010"
                     /inference="COORDINATES: protein motif:HMM:NF012242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEX4899670.1"
                     /translation="VQAGVLSGEKTDILLLDVTPLSLGIETLGGVFTKLIERNTTIPT
                     RKSEIFSTASDNQTSVEVHVLQGERQMAGDNRTLGKFHLVGIPPAPRGMPQVEVTFDI
                     DANGIVNVSAKDMGTGREQKITITSSSGLNKDEIDKMMRDAESHAADDAKKREEIEAR
                     NRLDGLVYQMEKTLNENREKAGTVASEVETAIAQGKTALQEGGVDKLNDAFNQLQAAS
                     HKLAEALYQQAPPPGAEPQAEADQASAAGAASGSGSGEDNVIDAEYVDVDENK"
BASE COUNT          572 a          829 c          785 g          709 t
ORIGIN      
        1 tgagccagtg ctggcgtggt gagcggtgag ggcttcatct gatttctcct gtgaggaagg
       61 ctcgcgctaa tggctgcgac ctaagagaga atctattttg aatgaaatcg ttaagtctcg
      121 agcgttcgtt acggctctaa tcaagagagt cgagcatcac tcaaaagtgt acggaggcta
      181 gcactgcggc gcggcgattc gcaatcgttc ccagccaaat cgtaatttca gatcactgca
      241 agatttcaat tagccacggg aaacggtggt gcggcggcgg agcacggcgg tgcgcgagac
      301 gcgcacgagt gcgtcagacg tgccttcggg gggtggttcg gtgaagttgg taaactcttc
      361 ggcgtgcgac tggagataaa gaaagaagcg ctcaaactcg tgctgcatcg catccatctt
      421 ctcgcgcatg ttcatgatga tttccacgcc ggcaagattc acgcccaggt cgcgagttaa
      481 ggaaaggatc aactcgagcc gctcgagatc ggaatcggtg tagaggcgcg tgttgccttc
      541 cgaacgcgat ggcttcaata gcccttcgcg ttcgtaaaga cgcaaggtct gcgggtggag
      601 actgtactgc tccgccaccg cgctgatcgt atatgtcttg ggcttgcgct tcatgtcatt
      661 tccgattgcc gattgccgat tgccgattgg catcttcggt ttcttatctt tttactcacc
      721 ctgttccaaa tctaactggt gccttccgcg aaagaccaat cgtcaatcgg caattggcaa
      781 tcgccaatct actccagtcc catttccacg cggggattgt cggagttcat tttcgcgtac
      841 tgtcgcagca actccttcgt ctcttccgaa atcactttcg gtaaaatcac ctgtacttct
      901 acaaactgat cgcccttgga tccagggttg cgcaagactg gcgcgccgcg ttcgcgtaaa
      961 cgaaactttt gtcccgactg cgtgcccggc ggaatacgca attgggcttt gccttcaacc
     1021 gtaggcactt cgatcttcgc tcccaatgca gcctccggca ccgtgattgg aactgtgacg
     1081 tagatgttgt cgcccttgcg cgtaaaatgt ttgtgcttac caacattcgt gatgatgtgc
     1141 agatcacccg cgggggctcc catccgtccg ccttcgccct tgccaggaat tctgacgcga
     1201 gaacccgttt ccacacctgc aggaatgcgc accttgaccg tttcagtctt gggtattgtt
     1261 cctttgcctt tgcataacga gcaaggagaa cggcgccgac ccgtgccttc gcagtcggtg
     1321 cattcctgag caaaacgcaa acgcccgccc gcacgttgca cctgaccgga gcccttgcaa
     1381 gtggggcaga tcatcaccgg tccgccgatg tcaccggcgc cgttgcagcg cgtgcattgc
     1441 tcggaacgat ttaccgtgat gttggttgtt aagcctgtca gcgcttcttc gaacgaaagc
     1501 gcgagcggca tttcgatgtc ggcgcctcgc ttgggctgcg gccgcggcgg ctcacgctct
     1561 ttcgtggcgc ccccgccgaa gagatcggaa aagatgtctt tgaaactcgt accgctgccg
     1621 gccccggaac tggtgcccca atcgaaacct gtgaaatcga atcccggaga ggcaccgccg
     1681 ctgctgctgc ctggagtcgc tccccgtgca gcggcatccg ccagattgtc ggagtattgg
     1741 ccaaagcgat cgtagacggc gcgctttttc gggtcggaaa gaacgtcatg agcttcggtt
     1801 gttaacttga aacgctcttc ggacgccttg tcgccaggat ttacgtcagg atggtactta
     1861 cgcgccagcc ggcgataagc cttcttgatt tcttcgggct tagcatcgcg ctttacgccg
     1921 agcgtttggt agtaatcctg tttcgccata gcctggtaac caataccaaa actgccgatt
     1981 gccgactgat cagtttctct aatccagcct aaacttcagc tgcgaattcg agtcactgac
     2041 cagtcggcaa ttggcaatct aaaacggcac aatgtgttta cttgttctca tccacatcga
     2101 cgtattcagc gtcgatgaca ttgtcctcgc cggaacctga gcccgaagcg gcgccagctg
     2161 ccgacgcttg atcggcctcc gcctgaggtt cagcgcccgg cggcggtgct tgctggtaca
     2221 gcgcctccgc tagtttgtgc gaagctgcct gcagctgatt gaacgcgtcg ttgagcttgt
     2281 caacgccgcc ttcctgtaaa gcagttttgc cctgggcgat tgccgtctct acttcggaag
     2341 cgacagtgcc cgctttctca cggttctcgt tcagagtttt ctccatctgg tagacaagac
     2401 cgtcgagccg gttgcgcgct tcaatttctt cacgcttctt ggcatcgtca gcggcatgcg
     2461 attccgcatc gcgcatcatc ttgtcgatct catctttgtt aagaccagaa gatgaggtga
     2521 tcgttatctt ctgttcgcga cccgtcccca tatctttcgc ggatacgtta acaatgccgt
     2581 tcgcatcgat atcaaaggtt acctcgacct gcggcatgcc acgaggtgcc ggcggaatgc
     2641 ccacgaggtg gaacttgcct aaggtgcgat tgtcgccggc catctgtcgc tccccctgga
     2701 gaacatgcac ttccactgat gtctgattgt cagaggcggt agaaaagatc tctgactttc
     2761 gcgtcggaat cgtggtgttg cgttcgatca acttcgtgaa cactccgccg agagtctcga
     2821 tgcccaaact aagcggagtt acgtcgagta gaaggatgtc ggttttctca cccgacagca
     2881 cgccggcctg aaccg
//
LOCUS       DASUEX010000238         3135 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_965471, whole genome shotgun sequence.
ACCESSION   DASUEX010000238 DASUEX010000000
VERSION     DASUEX010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3135
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_965471"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="VFV61_04015"
     CDS             <1..354
                     /locus_tag="VFV61_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899671.1"
                     /translation="AMICTSSEERTMPDNIIEEIEYPGDCTLEEINNDLVVKENVRTE
                     KLVELRIIPDGPDGHLSLASMADRPIGAFDRLVLVPVPDGDLESVIEEQAPKEFLFSA
                     TIWVEDEETEVAAFR"
     gene            667..1590
                     /locus_tag="VFV61_04020"
     CDS             667..1590
                     /locus_tag="VFV61_04020"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX4899672.1"
                     /translation="MSTLNLRGILLPFTTPFTKDEDLDTRGLRQNLLHWNQTGIAGYV
                     ALGSTGERVNLDEREYLEVLEIARAEVPENLTFIAGVGQQSTRQTINEVRKAAKAGAD
                     AALVITPSFYRSAITQPALVAHYTAIADASPIPLVLYSMPDLTGITIEPETTAKLSEH
                     PNIIGVKDSSNNVERLKETLRAVQDDFAVMTGNGTVLYDALLAGARGAILAVGCVAPE
                     MCLRIFAQVRQGNMTLAAALQEKLTPLALVVTKRYGIGGLKTAMDLTGLQGGSVRAPL
                     KSPSDEARREIAALLQAVTKENDPVGLAGAL"
     gene            1593..>3135
                     /gene="bshC"
                     /locus_tag="VFV61_04025"
     CDS             1593..>3135
                     /gene="bshC"
                     /locus_tag="VFV61_04025"
                     /inference="COORDINATES: protein motif:HMM:NF021586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis cysteine-adding enzyme
                     BshC"
                     /protein_id="HEX4899673.1"
                     /translation="MSTTEPACYSTPEEAGLRVETLPFDRIPHQSRLFLDYLRDPTSL
                     QRFYPSAVRYHHELSTRVPNVLSAHKTDRTLLCDALSEMNSRWGAGKETLANIELLRD
                     ADCVAVVSGQQAGLFTGPLYTIYKAISAVKLAGCLTQRNTKAVPVFWMATEDHDFPEV
                     ASAEFIGRDCRLTSVEAAAAMHPEGTPVGRVVLDGSINAVLEQLFERLPSSEFTADLE
                     SMLRESWKPGLGYGEAFARMMTLLLGRYGLILLDPINPELKKLAAPLYAEAALRSQEI
                     AEAIEVRSRELEGAGYHAQVMASQNSFPLFLHDESGARHAMTRIEDGRYRAKDSDEPY
                     KKEELAELALAQPERFSPNVTLRAVVQDFLLPTIAYYGGAAEIAYFAQTAEVYRLLDR
                     PATPILPRSSLTMVERHTGRTLEKYGLTPESLFAGAETVLARVVEEHLGTATAQQFMQ
                     AEETFKRELDELREKLNDVDPTLASALETGRRKINYQLEGLRTRFHRAQLSRDEAAQR
                     QLERAF"
BASE COUNT          730 a          881 c          859 g          665 t
ORIGIN      
        1 gccatgattt gtacgtccag cgaggagaga acgatgcctg ataacattat cgaagagatc
       61 gaatacccgg gcgactgcac acttgaagag atcaacaacg atcttgtggt gaaagaaaac
      121 gtgcgaaccg agaagctcgt cgagttgagg ataatccccg acggacccga tggccactta
      181 agcttggcgt caatggcgga tcggccgatt ggcgcctttg acaggttggt gctcgtgccg
      241 gtacccgatg gtgacctgga atccgttata gaagaacagg cacccaagga gtttctcttt
      301 agcgcgacca tctgggtgga ggacgaagaa acagaggttg cagcatttcg gtaaactgct
      361 ctggaaactc aaaacgcgac attaagagcc ggcaaaccag cactcctgac cggagaggcg
      421 attcgagttg agatcgttta attgaatccc tcaggcagga aagcagtcac tcaagcaaaa
      481 cccctctcag gtcaggaaaa ggggtttacc tccgccgagt tcgttttctc acacaggtct
      541 gggcggaata ctccacgcaa actgccattg tcaatcgtag gtaaaacccc ttcctccatt
      601 tcctgccctt ggcgcttcgt cgtagaatgc agaaactttg gttcattccc gtaaacgccg
      661 aacgccatgt caaccttgaa tcttcgcggc attctgctgc cgttcaccac acctttcaca
      721 aaagacgaag atctcgatac ccggggactt cgccagaatc ttctgcattg gaaccagacc
      781 ggcatcgcag gatatgtggc cctcgggtca accggtgaac gcgtcaacct cgatgagcgc
      841 gagtatctcg aggtgctcga gatagctaga gcggaagttc ctgagaatct aaccttcatc
      901 gccggtgtcg gacaacagag cacgcgccaa acaatcaatg aggtaagaaa agccgccaag
      961 gctggcgccg atgccgcact ggtgatcact ccgagtttct atcgctccgc aattactcag
     1021 ccggccctcg ttgcccacta cacagcaatc gccgacgcat cgccgatccc actggtcctt
     1081 tacagcatgc cggacctgac aggcatcacg attgaaccgg agacaaccgc caaactcagc
     1141 gagcacccaa acatcatcgg cgtgaaggac agttctaata acgtcgagcg cttgaaggaa
     1201 acgctgcgcg ctgttcagga cgacttcgcg gtcatgacgg gtaatggaac cgtgctctac
     1261 gatgctctct tagcaggtgc ccgaggcgct attctcgcgg tgggctgcgt tgcgcccgag
     1321 atgtgtcttc gcattttcgc ccaggtcaga cagggcaata tgacactggc agctgctttg
     1381 caggaaaaac ttacgccgct tgcactggtt gtgacgaagc gttacggtat cggtggtctt
     1441 aaaacagcaa tggatctgac cggattgcag ggtggttcgg tgcgtgcccc attgaagagc
     1501 cccagcgacg aagcacgacg ggaaatcgcg gccctgcttc aagcagtaac taaagagaac
     1561 gatccggtcg gcctcgcagg agcattgtga acgtgagcac tactgaaccc gcttgttaca
     1621 gcacacctga ggaagccggt ctgcgagtcg agaccttgcc tttcgatcgc attcctcatc
     1681 aaagccgcct cttcctggat tacctgcgcg atcccacttc cttgcaacgc ttttatccct
     1741 cagccgttcg ctatcatcat gaactttcca cgcgcgtacc caacgttctt tcggcgcaca
     1801 aaacagatcg gacgttgctc tgtgatgcgc tttcggagat gaactcgcgt tggggggccg
     1861 gcaaagaaac gctcgccaac atcgagctgc tgcgtgacgc agattgcgtt gctgtagttt
     1921 ctggccaaca ggctgggctg ttcacaggtc cgctctacac catttacaaa gccatctctg
     1981 cggtaaaact cgctggctgc ctgacacagc ggaacacgaa agccgtcccc gttttctgga
     2041 tggcgactga ggaccacgat tttccggagg tggcctcggc tgaattcatt ggacgtgatt
     2101 gcaggctgac aagcgttgaa gccgcggcag ccatgcaccc ggaaggaact ccggttggtc
     2161 gcgtggtgct tgacggctca ataaacgctg ttctcgagca gctatttgaa cgtttaccgt
     2221 cttcagaatt cacagccgac cttgagagta tgctccgtga aagctggaaa ccgggccttg
     2281 gttatggcga agcttttgcg cgaatgatga ctctgctgct cggccgttac gggctcatct
     2341 tgcttgaccc aattaacccc gaactaaaga agctggcggc gcctctctat gctgaagcag
     2401 ccctacggtc acaggagatt gctgaagcga tcgaagtgcg cagccgcgag ctggaaggtg
     2461 ccggttacca tgcgcaggtg atggcctcgc agaattcctt tcctctcttt ttgcatgacg
     2521 agtcaggcgc ccggcatgcc atgacgcgaa tcgaagacgg taggtaccga gcaaaggatt
     2581 cggacgagcc ttacaagaaa gaggaactcg ctgagctcgc tctcgcccag ccggagcgtt
     2641 ttagtcccaa cgtaacgttg cgggccgtag tgcaggattt cttgttgccg actatcgcgt
     2701 actacggtgg cgctgctgag attgcctact tcgcgcaaac agcggaggtc tatcgcctgt
     2761 tggaccggcc ggctactccg attctgcctc gctcaagcct caccatggtg gagcgacata
     2821 ccggaagaac ccttgagaag tatggtctga cgcccgaatc tctttttgcc ggagcggaga
     2881 ccgtgttggc gagagtagtg gaagaacatc tcgggactgc aaccgcccag cagtttatgc
     2941 aggctgagga aaccttcaaa cgcgagctcg acgaactgcg tgagaaacta aacgatgtcg
     3001 atcccacgct cgcttcggcg cttgagaccg gacgccgcaa gatcaattac caactcgaag
     3061 ggttgcgcac tcgctttcac cgggcacaac tctcacgcga cgaggcagct caacgtcaat
     3121 tggaacgggc gtttg
//
LOCUS       DASUEX010000239         5751 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_729149, whole genome shotgun sequence.
ACCESSION   DASUEX010000239 DASUEX010000000
VERSION     DASUEX010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5751
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_729149"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1887
                     /locus_tag="VFV61_04030"
     CDS             <1..1887
                     /locus_tag="VFV61_04030"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX4899674.1"
                     /translation="AILVGTFGLVLTRLRKLRNAAVQTEFPNLSLLISALLFAGWTAA
                     SVLWAANPYSAAHYAFTWGAYLLFFVLLHFVAARPRALRASFFALAAVVWLLSLSCLI
                     EWLFGAALNDHLYRANLKPLFRGFSGFGETMAVAAPIFGALALSLRKQRPAVLCGATS
                     LVAWLAVLQAFQRAPMLGAVAGFATLGAGILLFKRCRPRSTQRFALLLAGLVIVAVLQ
                     LVVFASIPGEEFTPLQRLKAVSTAEENTRVRLLYWSVGLEMFRAHPLLGVGANNYEVA
                     FPTARAQFAAAHPSSALVGLNEQLLTQYVHNEYVQILAELGAVGILLFGAFCTLLART
                     LWRALRHSRNPLLALGAAGGLLAFALSSAASAFSFRWFGSGLMCFFAAALVSHLAALS
                     SPAPQTESLEVPSFQRMLPAGAFAFSVLMLVGAGMQALNGVTHAWAQTSGTPFQAERL
                     YRQALRFNPFDAASHFDYGSLLYQQRRYDEAVPHLQYAVGHGFNTSTSYAVLAAAQEE
                     AGDVAASEQTLAGAAKAYPRSTFILVRHAAALSRLGKHEESEKRFADALAVDERAARG
                     WYQLINFDIDAALSAAQKDSSLAKPGELKPQTAIYVVLKENERRLNIPATSGWRGRVG
                     ALAN"
     gene            1911..2390
                     /locus_tag="VFV61_04035"
     CDS             1911..2390
                     /locus_tag="VFV61_04035"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEX4899675.1"
                     /translation="MKHANQQGFSLIELLIVVVIVGILSAIAIPNLLASRRAANEAST
                     LSALRTIHSCEVTYFSTVGNGDYTDLPTLALYTLTDRILGTGTKSGYSYVVNPTAIGV
                     RPSLFYTTAVPVTPSGLGQTGTRRFGMTEDGTLHGDSLALTPYASHALVRSAPTLAN"
     gene            2451..3248
                     /locus_tag="VFV61_04040"
     CDS             2451..3248
                     /locus_tag="VFV61_04040"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX4899676.1"
                     /translation="MYILKRMLGRIAAIAGNAFREAVRDRVLYNLVLFVLLLTGAAVF
                     IGELSGGQERKIIVDLGLSAMLLFGVFIAIFVGVGLVYKEIERRTVYTIFAKPVGRGE
                     FLLGKYLGLCLTLLVNVLVMGGGVCLALIYVSGGLDPLVFSIWPAILLLYVELMILTG
                     VALLFSSFSSPALSALLTFFVFIIGHFSADLKSLATSMGSVSARWFFGALYYVLPNLA
                     NLSFITPAAHGELPGGAYVASAIFYSLLYVTVVLAAATLIFSRRNFK"
     gene            3245..4156
                     /locus_tag="VFV61_04045"
     CDS             3245..4156
                     /locus_tag="VFV61_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899677.1"
                     /translation="MRRSLTTVDLLLLLTVLLGLAGVVGVARQIESQRSVAIATAPED
                     QLYLNGATVKRVSLGFNGLAADWYWMRSLQYVGRKLLTSGQGYQLDDLGALNLKLLPP
                     LLDTATTLDPQFMEPYEYAAIVLPGVDLKEAMRITQKGIAANPSSWRLYQHLGYIHWQ
                     QQDYEAARETYRAGAKIPGAPAWMEAMQARMAAAGGSRDTAREIYQRMYEQSDDETIK
                     EMARRRLLQLRSFDERDGLRRLMTAYKTQTGKCPGSWTELAPTLRALKVPLDASGAPL
                     DPSGVAYVVLADKCDVETDPRSEVPNN"
     gene            4165..4980
                     /locus_tag="VFV61_04050"
     CDS             4165..4980
                     /locus_tag="VFV61_04050"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4899678.1"
                     /translation="MPPIVEIENLTKDYEVGFWRKRKVRALDGLTLQVNEGEIFGFLG
                     ANGAGKTTTLKLLMRLMFPTGGNARILGHDIADITMHNQIGYLPENPYFYDYLTAREF
                     LDYCGQLFGSAQPARKQRTAELLARVNLDKKSWDVQLRKFSKGMLQRVGLAQALVNDP
                     AIVFLDEPMSGLDPVGRREVRDLIAGLRQEGKTVFMNSHILSDIEVLCDRVAILKRGR
                     LAHVGHLDELRRRAGEPNRVEVVATGTQAETLLLLLPDRDKIPGKFDGKRVTY"
     gene            complement(5138..5641)
                     /locus_tag="VFV61_04055"
     CDS             complement(5138..5641)
                     /locus_tag="VFV61_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899679.1"
                     /translation="MSSSERDLEGSDDSRRKIIIGAAVVAALFIAIIFYFLMRASSGG
                     NVEPVLEGALRPGSPEFDQNIAKLYLDPPEADEAKRALGDIVMNLQTTVRNFTGRTIN
                     GLEVKAAVVDGEGKPVKQRTVVVIPAKQAELQPNGTMLVVVRLDGMKDSDHRANIKME
                     VTGLKFK"
BASE COUNT         1176 a         1596 c         1567 g         1412 t
ORIGIN      
        1 gcaatactgg tgggcacgtt cggactggtt ctcacgcgac tgagaaaact caggaacgcg
       61 gctgtgcaaa ctgaatttcc aaacctctct ttgctgatca gcgcacttct atttgccggc
      121 tggactgccg cctcagtatt gtgggcggca aacccatatt cggccgcaca ctatgccttc
      181 acctggggcg cctatctgct cttctttgtg ttattgcatt ttgttgctgc ccgcccccgc
      241 gcgctgcgag cctcgttctt cgctttggcc gccgtagtct ggctgcttag tctctcttgc
      301 ctgatcgaat ggttatttgg cgcagctctg aatgaccacc tttaccgcgc gaacctcaaa
      361 ccgctttttc gaggctttag tggtttcggc gaaaccatgg cagttgccgc tcccatcttt
      421 ggcgcactgg cgcttagctt gcgaaagcag cgtccagccg tgctttgcgg cgccacctca
      481 ttagtggcct ggctcgccgt tctccaggca ttccagcgag cgccgatgtt gggcgccgtc
      541 gctggatttg caacgcttgg cgcaggcatc ttgcttttca aacgctgtcg tcctcgcagc
      601 acgcagcgct tcgctctctt gctcgcaggt ttggtgatcg tcgccgtctt acaacttgtc
      661 gtcttcgctt cgattccggg agaagaattc actcccctgc agcgactaaa agccgtctca
      721 acagcagagg aaaatacgag ggtccgtctg ctttactgga gcgtcggtct ggaaatgttc
      781 cgcgcacacc ccctcctcgg tgtcggtgcg aacaattacg aggtggcttt ccctaccgcg
      841 cgcgcacagt tcgcagcggc tcatccgagt agcgcgctag tgggattgaa tgaacagttg
      901 ctcacccagt acgttcacaa tgagtatgtg cagatccttg cagaactggg cgctgtcgga
      961 attctactgt tcggtgcttt ttgtaccttg cttgctcgca ctctttggcg cgccctccga
     1021 cattcacgga atcccttgtt ggcgttgggt gccgcgggcg gcttgctagc attcgctctc
     1081 agttcagccg ctagcgcttt ttcgtttcgc tggttcggaa gtggtctgat gtgctttttt
     1141 gcggccgcac tcgtttcaca tctcgccgcc ttgagttcgc cagcaccgca aaccgagagc
     1201 ttggaagttc cctcctttca gcggatgctc ccggcaggcg catttgcttt ttccgtgctg
     1261 atgctcgtcg gagccggcat gcaagcgctg aacggcgtca ctcatgcctg ggcccagacg
     1321 agtggaacgc cttttcaggc cgaacgcctt tatcgccagg cactccgatt caatccattc
     1381 gacgcggctt ctcatttcga ttacggctcg ctgctttatc agcaacgccg ttatgacgaa
     1441 gccgtacctc acttgcaata cgccgtgggc catggcttca acacttcgac cagctatgca
     1501 gtattggctg ctgcccagga agaagcgggc gacgtggcgg cgtcagaaca aactctggcg
     1561 ggcgctgcga aagcctaccc gcgttcaact ttcattctag tgcgtcatgc tgctgctctc
     1621 agtcggctgg gcaagcatga ggaatcggaa aagcgatttg ccgatgcgct ggccgtggat
     1681 gagcgtgctg cacgtggctg gtatcaacta atcaacttcg acatagatgc tgctctcagc
     1741 gccgcgcaga aggactcgag tctcgccaag ccgggcgagc tcaagccgca aactgcaata
     1801 tatgtggtgc tgaaagagaa tgagagacgt ctgaacatac cggcaactag cggttggcgt
     1861 ggtcgcgttg gcgcgttagc caactaattc cattgaaccc gacaaacatc atgaaacacg
     1921 caaatcaaca agggttttcg ctgattgaac tgctgatcgt ggtagtcata gttggaatcc
     1981 tgtcagcaat agcgattccc aatctgctcg cctcacggcg cgccgccaat gaagcctcaa
     2041 cactgtcagc gttacgcacc attcatagct gcgaggttac ctatttctca actgtaggca
     2101 acggagacta tacggacctg ccgacgctcg cgctgtacac cctcaccgat aggattcttg
     2161 gcaccggaac caagagtggt tacagctatg tggtcaatcc gactgccatc ggggtccgtc
     2221 cctcactctt ctatacgaca gcagtgccgg tcactcccag cgggctgggg caaaccggaa
     2281 cccggcgttt tggtatgact gaagatggaa ccctgcatgg cgattccttg gcgcttacgc
     2341 cctacgcgag ccatgcttta gtccggtctg caccaacact cgcaaactag atccttctgc
     2401 tacgaagtta tgcccctcaa gacattggtg ctgacacgca aagttcgccc gtgtatattc
     2461 tgaagagaat gctgggccgc atagcagcaa tcgccggcaa tgcttttcgc gaagcggtgc
     2521 gagatcgcgt gctttacaat ctcgtgcttt ttgttttgct cctcactggt gccgccgtct
     2581 tcatcggcga actttccgga ggacaagaac gaaaaatcat tgtcgatctg ggtctcagcg
     2641 cgatgctgct gtttggcgtc ttcatcgcga ttttcgtcgg cgtgggcctg gtctacaaag
     2701 agatcgaacg gcggacggtc tatacaatct ttgcgaagcc cgttggccgg ggagaatttt
     2761 tactgggtaa atatctgggg ctctgcctga cgctgcttgt gaacgtgctg gtcatgggtg
     2821 ggggcgtttg tctcgccctc atttacgtaa gtggcggttt ggacccgctc gtgtttagca
     2881 tctggccggc gattcttttg ctctacgtcg agctgatgat cctcaccgga gtcgcgctgc
     2941 tcttttcttc cttctcttca cccgcgctct cagcgctgct cacgtttttc gtcttcatca
     3001 ttggccactt cagcgccgac ctcaagagtc tggctacttc aatgggcagc gtttccgccc
     3061 gctggttttt cggcgccttg tattacgtgc tgcccaacct cgccaatttg agttttatca
     3121 cgccggctgc tcacggtgag ttgcctggag gtgcttacgt cgccagcgcc atcttttact
     3181 cacttctcta tgtaaccgta gttttggcgg cagccacctt gattttcagc cggcgcaatt
     3241 tcaaatgaga cgaagcttga ccacagtgga tctcctgcta ctcctaacag ttcttctggg
     3301 actggcaggc gtagttggag ttgcccgcca gatcgaatca caacgctccg tcgccattgc
     3361 aaccgcaccc gaagaccagc tttatttgaa cggtgccacg gtcaagcgcg tcagcctggg
     3421 tttcaatgga cttgcggccg actggtattg gatgcgatca ctgcagtacg tcggcaggaa
     3481 gctgctgact agtggacaag gttatcagct cgatgatttg ggagcgttga atctcaaact
     3541 gctgccaccg ttactcgata cggccacaac tctggatccg cagttcatgg aaccctatga
     3601 atacgctgcc atagtcttgc cgggggtgga tctaaaggag gcgatgcgca tcactcagaa
     3661 ggggatcgca gctaatcctt cgtcatggcg actttatcaa caccttgggt acatccactg
     3721 gcagcaacag gattacgaag cagcacgcga aacatatcgt gctggggcca aaattcccgg
     3781 tgcacctgcc tggatggagg cgatgcaggc gcggatggca gcagcaggag gcagccgtga
     3841 tactgcgcgc gagatctatc aacgcatgta tgagcagtcc gatgatgaaa caattaagga
     3901 gatggcccgg cgccgtttgc tacagctccg ctcgtttgac gagcgtgatg gcctgcgccg
     3961 cttgatgacg gcttacaaga cgcagactgg taaatgtcca ggttcgtgga cagagttagc
     4021 acccactctc cgcgcgctga aggtcccgct cgatgcttcc ggagcacctt tggatccttc
     4081 cggagtcgct tatgtggtgc tggcagataa atgtgatgtg gaaaccgatc cccgttctga
     4141 agtgcccaac aactaaccaa gaccatgccg ccgattgttg aaatagaaaa cctgacgaaa
     4201 gactacgaag tcggattttg gcgcaagcgg aaagttcgcg ccctcgacgg tctcacgttg
     4261 caagtgaatg agggcgagat ctttgggttc ctgggggcaa acggagcggg caaaaccacc
     4321 acgctgaaac tcctgatgcg gttgatgttt cccaccggag gtaacgcgcg cattcttggg
     4381 catgacatcg ctgacattac gatgcacaac caaattgggt acctgcccga gaacccttac
     4441 ttttatgatt acctgacggc gcgggagttt ctggactact gtggtcaact ttttggcagt
     4501 gcccaaccag ctcggaaaca gcgcactgcg gaactcttgg cgcgagtaaa tctcgacaag
     4561 aaatcgtggg acgtacaact gcgcaagttt tccaaaggaa tgctgcaacg cgttggtctc
     4621 gcgcaggcgc tggtgaacga tcccgcgatt gtctttctcg atgagccaat gagtggtttg
     4681 gacccggtgg gccgcagaga agtgcgtgac ttaatcgccg ggttgcgaca agagggcaaa
     4741 accgttttca tgaactcgca cattctgtct gacattgaag tgttatgcga ccgggtagca
     4801 attcttaaac gaggcaggct ggcgcacgtt ggccacttgg atgaattgcg acgacgggcc
     4861 ggggaaccca atcgggttga agtggtagcc acgggaacgc aagctgaaac tctgttgttg
     4921 ctgctaccgg accgcgacaa aattccaggt aagttcgacg gcaagcgggt tacgtattga
     4981 actagcggat gagcagtatg ttgatgaagt acttgctgcg ctgcggaacg ctgggggaaa
     5041 gcttgtttcg gtccagccag tgaggcagtc tttggaagat ttgtttcttg agtcgccggt
     5101 tgaagaggcc gccgctagtt agttctgaca gcactggtca cttaaacttc aaaccagtga
     5161 cttccatctt gatattggcg cggtgatcgg aatctttcat tccgtccaac cgcacgacaa
     5221 ccagcatcgt accgtttggt tgtaactctg cctgcttagc ggggatcacg actacggtgc
     5281 gctgcttgac aggtttgccc tcaccatcga ctactgcggc cttcacttcc aaaccattta
     5341 tcgtgcgtcc ggtgaaattg cgcacggtag tttgcaggtt catcacgata tcgccgaggg
     5401 cgcgtttcgc ttcgtccgct tccggaggat ccagatagag cttggcgata ttttgatcaa
     5461 attccggcga gcccggacgg agagcgcctt ccaaaaccgg ctcgacattg ccgccgctgc
     5521 tggctcgcat caggaaataa aaaatgatgg caatgaagag cgcagcaact accgccgcac
     5581 caataataat ttttctccgc gagtcgtcgg agccttccaa atctctttct gaactactca
     5641 taatttgaac agcgcaattg taggatcgtg aatatggttt tgcaaagagc atagtccagc
     5701 gtcactccaa gtcgtgaccg tttagacccg tagtcagtgc gctttgttcc t
//
LOCUS       DASUEX010000240         4031 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1080112, whole genome shotgun sequence.
ACCESSION   DASUEX010000240 DASUEX010000000
VERSION     DASUEX010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4031
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1080112"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1900)
                     /locus_tag="VFV61_04060"
     CDS             complement(<1..1900)
                     /locus_tag="VFV61_04060"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional serine/threonine-protein
                     kinase/formylglycine-generating enzyme family protein"
                     /protein_id="HEX4899680.1"
                     /translation="MKECPACRRCFPDDINHCPQDGDATTQSLLGEPILDARYQLERR
                     LGQGGMGVVFQARHIFLKTAHAIKVILPDLVGNDPMLVTRFRQEALAAAAIRHPNIIA
                     VTDFGVVRGTMPFLVMEFVSGKSLQELLAEEGAMPPRRALELMNAIGAGVSAAHRMNI
                     VHRDLKPLNIMTQDNMSVAEGVKILDFGLAKIKSGELLGSFVQAKTTGLMGSPFYMAP
                     EQWSDEEPDARADVYSLGIILYQMLAGDVPFKGSSIPSIMKKHLTMPPPSFQSIGVQV
                     APAIEAVVRHALEKDVAARTPSVGEFLIELRNAVNATPDTRTPLSRDTGPVDPNRTLV
                     SPLSGTDPEVKAGQTTNVQAPAESVSALGNQTGIGEGGQTGNVTAAEKYRLEQEARDQ
                     AAREELAREAEARREAQDAAARTRKAEEDRLRKEREEKEEREREQREQLERVARQASE
                     LEAKLARLSTSMTPESGTVDPEATQLHRSSANSSSGTAPGIADRAGRVHVEIAPKPRN
                     TALYAGAAALVAILVIGGAITAYVMTRSKAIVTDPERPTPSPTAPTTPSFKPDLVEIP
                     GGTFQMGRNDGPTQERPAHPVTVKTFLLDRNEVVNGEYAEFVRETNGVAPSHWPNGKQ
                     IFGQENWPV"
     gene            complement(2149..>4031)
                     /gene="leuS"
                     /locus_tag="VFV61_04065"
     CDS             complement(2149..>4031)
                     /gene="leuS"
                     /locus_tag="VFV61_04065"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEX4899681.1"
                     /translation="IDFKIKDSGSQIRVFTTRIDTVFGATALVLAPDHPLIGELLKGS
                     ALRGDVEKFAEKVAADRKRPADATGEEIKEGLNTGALAVNPFSGETIPVWVANYVLMD
                     YGTGAVMSVPAHDERDFEFAQKYSLPVRQVIAPITHNQIAAEPQSVTGTDQSAEMRNA
                     FTEYGVLINSGEWGGKPSAVAIKEMAQYAEAKGFGGGAVTYRLRDWGISRQRFWGAPV
                     PIINCVKCGSVAVPYEDLPVLLPERAEFTGSGESPLAGVAEFVNTTCPQCGGPARRET
                     DTMDTFVDSSWYFFRYCDPRNDNAPFDPELVKQWIPVDQYIGGDSHAVLHLIYTRFWN
                     KVMRDLGLVSFAEPVKKLLTQGMVTNRLEGTDEWKAMSKSLGNGVDPDEMIEAFGADA
                     ARLFILFAAPVENELRWNESGIEGAVRFLRKVYTMVWRWHERLTDPGQRIEAAQAGEF
                     SAGARALRRKTHQTIARITNDFEHLHLNTSVAALMELFNELAAFEVQPATASPSDLFA
                     VREALESLAIMLAPFCPHVAEELWEQLGHRGGLLAQAIAPKIPTWPQAEAEWARKEEL
                     EIPIQVNGRLRGRVLAAPDVSEEDLRAAALADEKVRSFTDGHEVVKVVVVPRRLVNIV
                     VR"
BASE COUNT          811 a         1222 c         1105 g          893 t
ORIGIN      
        1 cgacaggcca gttctcctga ccaaagattt gtttgccgtt cggccaatgg gaaggcgcga
       61 caccgttagt ctcgcgcaca aactccgcgt actcaccatt cacaacttca tttcgatcga
      121 gcaagaacgt cttcactgta accggatgag cgggcctttc ctgggttggg ccatcgttgc
      181 ggcccatttg aaaagtgcct ccgggaatct cgaccaggtc cggcttgaat gatggcgtgg
      241 ttggtgcggt tggcgaaggt gtcggccgct caggatcagt gacaatggct ttcgatctag
      301 tcatcacgta agcggtgatc gcaccgccga tgaccaggat ggcaaccaga gcagcagcgc
      361 ccgcatagag agctgtgttg cgcggtttcg gagcaatctc aacgtgtacc cttccagctc
      421 gatccgcaat tccgggagcg gtacccgaac ttgagttggc gctcgatcgg tgcaactgcg
      481 ttgcctcagg atctactgtt cccgactcgg gcgtcatgct cgtggagagt cgcgcgagtt
      541 ttgcctccag ttcactggcc tgacgcgcca cacgctccaa ttgctcccgt tgttctcgct
      601 cgcgctcttc cttttcctcg cgctctttgc gcaaccgatc ctcttcggcc ttcctcgtgc
      661 gcgcggcagc gtcttgtgct tcgcgcctgg cctcggcctc gcgcgctaac tcctcacggg
      721 cggcctggtc acgcgcttct tgttccaaac gatatttctc ggccgcagtt acattgcccg
      781 tctgtccgcc ttcgccgatg ccagtttggt ttcccagcgc tgagaccgac tcggcgggcg
      841 cctggacgtt ggttgtctgt cccgccttaa cttcgggatc ggtgccggag agaggcgaaa
      901 ccaaagttct gttcggatcg acgggcccag tatcgcgaga aagcggagtg cgcgtgtcgg
      961 gagtcgcatt aacggcgttg cggagttcga tcagaaactc accgacggac ggagtgcgcg
     1021 ctgcgacatc cttctcgaga gcgtgtcgca cgactgcttc aatcgccgga gccacttgca
     1081 ctccgattga ttgaaagctg ggtggcggca tcgtcagatg ttttttcatg atcgacggaa
     1141 tcgacgaccc tttgaaaggc acgtcgcccg cgagcatttg atagagaatg atccccagac
     1201 tgtaaacgtc ggcgcgcgca tcaggctctt catccgacca ctgttccggc gccatataga
     1261 atggcgaccc catcagtccc gtcgttttgg cctgcacgaa tgaacccaac agctcgccgg
     1321 atttgatctt ggccaatccg aagtcgagaa tcttcacacc ttcggcaacg gacatgttgt
     1381 cctgcgtcat gatgttcaga ggcttcagat cccgatgcac gatgttcatg cggtgggctg
     1441 cggagacacc cgcgccgatt gcattcatca attcaagcgc ccgccgtggt ggcattgctc
     1501 cctcttcggc gagcagctcc tgcaacgact tgccacttac aaactccatc accaggaacg
     1561 gcatggtgcc gcgcacgacg ccaaagtcag tgacagcgat gatgttggga tggcgaatgg
     1621 cggcggcggc cagcgcctcc tggcggaagc gtgtgacgag catcggatcg ttgccgacga
     1681 ggtccggcaa gatcactttg atggcgtgtg ctgtcttaag aaagatatga cgcgcttgaa
     1741 agacgacacc cataccgccc tgaccgagtc gtctttccaa ttgataacga gcgtcgagga
     1801 taggttcgcc cagcagcgat tgagtggtgg catcgccatc ctgaggacaa tgatttatgt
     1861 catcggggaa gcagcgcctg caggctggac attctttcat tgaaaggctc gaaggggtct
     1921 cagggactac ccgcttaagg actggaagct gatcagtagc ccacgcgctc tccgcacggc
     1981 tgaaaggtgc aaacgcgctc tgtgattcta actaacactc tccccgagtc gcaacaagcg
     2041 attgaaatca ttccccatgt ttctggcagg tgggaactct aactgaacag ttttaacgat
     2101 cggagggccg cgccttcggg gcaagcctcg tagcaactac cctccagctc aacggacgac
     2161 gatgttcacg aggcggcgcg gaacgacaac cacttttacg acctcatgtc cgtcggtaaa
     2221 gctacgaacc ttttcatccg cgagtgctgc ggcgcgcaaa tcttcttcac taacatccgg
     2281 ggcggccagc acgcgcccac gcaggcgacc atttacctga atcggaatct ccagctcttc
     2341 ctttcgagcc cactctgctt ccgcctgcgg ccaggtggga atcttcggcg ctattgcttg
     2401 cgccaacaag ccgcccctgt gtcccagctg ctcccagagt tcctccgcta catgcgggca
     2461 gaaaggcgcc agcattatgg ccagcgactc cagcgcttca cgcacggcaa acaaatcact
     2521 cggcgaggca gtagccggct gaacctcaaa ggcagccagt tcgttgaaca gctccatcaa
     2581 agccgccaca ctcgtgttta agtgcaaatg ttcgaagtcg ttggtgatgc gagcaatggt
     2641 ttgatgagtc ttacgtctca gcgcgcgcgc gccggcgcta aactccccgg cttgcgccgc
     2701 ttcaattcgt tgccctgggt cggtaagtcg ttcgtgccag cgccacacca tcgtatagac
     2761 cttgcgcaga aagcgaaccg caccctctat gccggattcg ttccaacgca actcgttttc
     2821 gaccggggca gcaaacaaaa tgaaaagccg cgctgcatcg gcgccaaacg cttcgatcat
     2881 ttcgtccgga tcgacaccgt tgcctaaact cttggacatt gctttccatt cgtcggtgcc
     2941 ttccagtcga ttagttacca tgccttgcgt caacaacttc ttcaccggtt cggcaaatga
     3001 gactaatccc aggtcgcgca ttaccttgtt ccaaaagcgt gtgtagatca ggtgcaagac
     3061 tgcgtgagaa tcgccgccga tgtattgatc cacggggatc cattgtttga cgagctcggg
     3121 atcaaacggc gcgttatcgt tacgtggatc gcagtagcga aagaagtacc aggacgaatc
     3181 gacgaaggtg tccatcgtat ccgtctcgcg gcgggccgga ccgccacact gcggacacgt
     3241 agtgtttaca aactccgcga cacctgccaa tggtgattcg ccgctaccgg taaactccgc
     3301 cctctcggga agcaggaccg gcaggtcttc atagggcacg gccactgaac cgcacttgac
     3361 gcagttgata atcggcactg gcgcgcccca gaaacgctgc cgcgagattc cccagtcgcg
     3421 taagcggtaa gtcacggccc cgcccccaaa tccttttgct tccgcgtact gcgccatttc
     3481 tttgatagcg actgcggaag gctttccgcc ccactcgccg ctattgatca gcacgccata
     3541 ctcagtaaac gcgttgcgca tttcggctga ttggtccgtg ccggttaccg attgcggttc
     3601 ggccgcaatc tgattgtgtg ttatgggcgc gattacctgg cgcactggca acgaatactt
     3661 ctgcgcgaat tcaaagtcgc gctcgtcgtg ggcgggaacg ctcatcaccg cgccggtgcc
     3721 gtaatccatc agcacatagt tggcaaccca gacgggtatt gtttcgccgc tgaacggatt
     3781 aaccgccagg gctccggtgt tcagtccctc cttgatttct tcccctgtgg catcggctgg
     3841 acgcttacga tcggcggcaa ccttctcggc aaacttctca acatcgcctc tcaatgccga
     3901 ccctttaagc aattctccga tcagtggatg atctggggcc agcaccagag ctgtggcacc
     3961 gaagacagtg tcgatacgcg tggtgaaaac tcggatctgg ctgccgctgt ccttgatctt
     4021 gaaatcgatg t
//
LOCUS       DASUEX010000241         4651 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_729242, whole genome shotgun sequence.
ACCESSION   DASUEX010000241 DASUEX010000000
VERSION     DASUEX010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4651
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_729242"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="VFV61_04070"
     CDS             <1..508
                     /locus_tag="VFV61_04070"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEX4899682.1"
                     /translation="QYWIHRYDAVIRRQAAIYRLDPDLVWSVIYEETYFRPWKLGKAG
                     EIGLMQVTPGVGRDWASETGMTEFERQMASDPAALLSDPERNIQIGCWYLEKFYETYR
                     DMPDAEARMIAAYNAGPTRAAEWNRAEPGRPLTGPEYIERINIPSTRAYVTSILERYR
                     KKKGVTSQ"
     gene            550..1896
                     /locus_tag="VFV61_04075"
     CDS             550..1896
                     /locus_tag="VFV61_04075"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX4899683.1"
                     /translation="MTTLYSARWVLPVSSSPIVDGALAIEEAAIVGVGSYAELARRYP
                     EAERRSYGEAAILPGFINAHSHLELTAMRGFLDSVERDFFSWLRRLTVARLEHMTAND
                     LQASATWGACEALRAGVTCVADASDSAAESMKALRETGLRGTVYQESFGPDPKFASEN
                     FAKLTEKVSRLKTLQTSLVQVGVSPHAPYTVSAPQLEMISEFALAGRLPVMMHAAESV
                     AERMLLAEGSGSFAEGFASRGIEWSAPSVSTIRYLARHGILQTQPLLAHCINVDADDL
                     DLIGETQTRIAHCPKSNAKLGHGRAPFAEFLRRKLSVGLGSDSVASNNTCDLLEEARF
                     ALLLARAVDGPNNLSASDALHAATLGGARALGLEGVVGELREGCQADFAVVSLAGTHQ
                     LPSYDPVSCLIFSSSGRDVLLTVVAGKELYREGSLAGVDEERLRARMNEIKVGLDQ"
     gene            complement(2038..3411)
                     /locus_tag="VFV61_04080"
     CDS             complement(2038..3411)
                     /locus_tag="VFV61_04080"
                     /inference="COORDINATES: protein motif:HMM:NF004426.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl ornithine aminotransferase family
                     protein"
                     /protein_id="HEX4899684.1"
                     /translation="MSSTTMAPPRRPLIKTALPGPKGQVIIEADAQFVNPSYPRPSFK
                     LVAERAQGVWVEDVDGNIFLDCNAGVAVCSTGHCHPEVVQAIQRQAEQLIHMCGTDYY
                     YSLMPALAQKLDEIVPVPSPTRTHFANSGTEAVETALKLAMHATGREKFIAFFNSFHG
                     RTLGSLSLTSSKVAQRLGFKRQALDVVHVPFPNEFRNPFRAEDCGNGGAGQGALHWIE
                     ERLFKTTTPPQEVAAIVVEAVQGEGGYVPAPKNFLLGLRRICDEHGILLVVDEVQSGM
                     GRTGKMFASDHHELKPDIVCIAKGIGSGLPIGATVARASLMDWKPGAHASTFGGNPVC
                     IAAALKTIELLQGGLVQNSAAVGAYLKGELTKLMQKYDCIGDVRGMGMMIGVEFVTDK
                     QTNTPAPELRDRVEVACFERGLIILGAGTNTIRWSPPLILTKENVDVAVEIFDEAIAA
                     AVGQKQR"
     gene            complement(3741..4160)
                     /locus_tag="VFV61_04085"
     CDS             complement(3741..4160)
                     /locus_tag="VFV61_04085"
                     /inference="COORDINATES: protein motif:HMM:NF017935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HEX4899685.1"
                     /translation="MKRIRPPLSKALAWCLALRCPVCGRSRIFARLFQIEERCAACGA
                     VFKREEGFFVGAILVAVVTTEAVIVLVYLISLPIVAAHYQLVIGVLLGLALIFPVLFF
                     HHSWSIWLSFDHFIEGLPQEPAGLQTSLPNDRGARKE"
     gene            complement(4231..>4651)
                     /locus_tag="VFV61_04090"
     CDS             complement(4231..>4651)
                     /locus_tag="VFV61_04090"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX4899686.1"
                     /translation="GNRAVKLESVAACSFNGNEQLLRRAIENVVRNAVHYTAEGTEVE
                     IEVRETSTETNARVLTITVRDHGPGVPDAALTEIFRPFYRVDDARDREAGGVGLGLAI
                     ADRAIQLHGGTVTAANAIGGGLIVTISLASTLNSGAV"
BASE COUNT         1048 a         1266 c         1331 g         1006 t
ORIGIN      
        1 tcaatactgg attcatcgtt acgacgctgt gatcaggcgt caggcggcaa tctatcgact
       61 cgatcctgat ctggtttgga gcgtcatcta tgaagagacc tacttccggc cgtggaagtt
      121 aggcaaagct ggtgagatcg gcttgatgca ggtgactccg ggggttggtc gcgattgggc
      181 aagcgaaacg ggtatgaccg agtttgagcg ccagatggca agcgatccgg cggctctcct
      241 cagcgatcct gaacgcaaca ttcagattgg ctgttggtat ctggaaaagt tttacgaaac
      301 ttatcgcgat atgccagacg ccgaggcgcg catgatcgct gcttacaatg ccggaccgac
      361 gcgtgccgcc gaatggaatc gggcagaacc tggtcgcccg ctgactggtc cggagtacat
      421 tgaacgcatc aacattcctt ccacacgtgc gtatgtcact tcgatcctgg agcgctatcg
      481 caagaaaaag ggggtcacgt cgcagtgaat caactactgg ggccctttcc caaatcccgc
      541 agcaactaga tgacgactct ctacagcgcg cgctgggtct tgccagtttc gtcaagtccg
      601 attgtcgacg gcgccctcgc tatcgaagaa gcagcgatcg tgggcgttgg aagttatgcc
      661 gagctggcgc ggcggtaccc cgaagctgaa cgccggtcgt acggcgaggc ggcgattctt
      721 ccgggattca tcaacgcaca ctctcatctt gaacttaccg cgatgcgagg gttcctcgac
      781 agcgtggaac gcgacttttt ttcgtggtta agaagactca cagtcgcccg gctcgaacac
      841 atgacggcaa acgatctgca agcttctgcc acttggggcg cgtgtgaagc cttgagagct
      901 ggagtcacat gtgtcgctga tgcgagcgac tctgccgccg aaagcatgaa ggcgttgcgc
      961 gagacaggac tgcgcggcac tgtgtaccag gagtcgtttg gtcccgatcc aaaattcgcg
     1021 tcggagaatt tcgcgaagtt aactgagaag gtgtcgcgcc taaagacgtt gcagacctcg
     1081 ttggtgcaag tcggcgtttc gcctcacgcg ccctacacgg tgagcgcgcc gcagctggaa
     1141 atgatttccg agtttgcgct ggcggggcga cttccagtga tgatgcatgc agcggaatca
     1201 gtcgctgaaa ggatgctgtt agctgaaggg agcgggagct ttgcggaagg ctttgccagt
     1261 cgcgggatcg aatggagcgc gccgtcagtt tccacgatca ggtatctggc gcggcatggc
     1321 atcttgcaga cacagcctct gctggcgcat tgcataaatg tcgatgctga cgacctcgac
     1381 ctgattggcg aaacgcaaac gcgcattgcg cactgtccca agtccaacgc gaaactcggt
     1441 catggccggg cgcccttcgc ggagtttctg cgtcggaaac tttcggtggg cctgggaagc
     1501 gattctgtag ccagtaacaa cacctgcgat ctgttggaag aagctcgctt cgctctgctc
     1561 ctcgcgcggg cggtcgatgg accgaataat ctcagtgcca gtgatgcact acacgcggcg
     1621 acattgggcg gcgctcgagc cctgggactc gagggcgtcg tcggcgaact ccgtgagggt
     1681 tgccaggcgg acttcgctgt ggtttcgctt gccggaaccc atcaactgcc gtcgtacgat
     1741 ccggtcagtt gtctcatctt ttcgtccagc ggacgtgacg tgctgctgac tgtggtggcg
     1801 ggaaaggagt tgtatcgcga gggaagctta gcaggtgtcg atgaagagcg actccgcgcg
     1861 cgaatgaatg aaatcaaggt tggtcttgat cagtgatgca aaactgtact tggaactttg
     1921 tacttagtac ttagttcgct ggtccggctt gacgcaactt cgcgtcatag ctcgaggtaa
     1981 ttaacaagaa caaagtacaa agcacttagt tccaagtaca aagagcaggg cgcaatctta
     2041 acgctgcttc tgtcccaccg ctgccgctat cgcctcgtca aatatctcga cagcaacgtc
     2101 aacattttcc ttcgtcagga tcagtggcgg cgaccagcga atcgtattcg tacccgctcc
     2161 cagaataatt aatccccgct caaagcaggc gacctcaaca cgatcgcgca gttccggagc
     2221 aggtgtgttg gtctgcttgt cagttacaaa ttcgacgcca atcatcatgc ccataccgcg
     2281 aacatcgccg atgcagtcat acttttgcat caactttgtc aactcgcctt ttaagtaagc
     2341 gcctaccgcg gcgctgtttt gcaccaggcc accctgcaac agctcaatcg tttttagagc
     2401 ggcggcaatg caaaccggat ttccgccgaa ggttgaagcg tgcgcgccgg gcttccaatc
     2461 catcagcgat gcacgtgcaa ctgtagcgcc gatgggcaaa cctgacccga tacccttagc
     2521 gatacaaacg atgtccggct tgagttcatg atgatcgctg gcgaacattt taccggtgcg
     2581 gcccatgccg ctctgcactt catcaacaac caaaagaatc ccgtgttcgt cgcagatgcg
     2641 gcgcaggccg agcaagaagt ttttcggggc cggcacgtat ccaccttcac cttgaaccgc
     2701 ctcaacgacg atggccgcaa cttcctgcgg cggcgtcgtg gttttgaaca aacgttcctc
     2761 aatccaatgc agcgccccct ggcctgcacc gccgttgcca cagtcttcgg cacgaaacgg
     2821 attccgaaac tcgttcggaa agggaacgtg cacaacatcc agcgcctggc gtttgaagcc
     2881 gagacgttgc gccacctttg aggaagttaa ggatagcgag ccgagtgtgc gtccgtgaaa
     2941 actattgaag aaggcaataa acttttcgcg cccggttgca tgcatcgcca gcttcaatgc
     3001 cgtctcgacg gcttcggtgc cgctgtttgc aaagtgtgtt cgcgtcggcg acggaaccgg
     3061 cacgatctcg tcaagcttct gagcgagggc gggcatgaga ctgtagtaat agtcagtccc
     3121 gcacatgtga attaattgtt cggcctggcg ttgaatcgcc tggactactt ccggatgaca
     3181 gtgtccggta gagcagacgg cgactccggc attgcagtcc agaaagatgt tgccgtccac
     3241 gtcttcaacc caaacgccct gagcgcgctc cgcaacgagc ttgaaagaag ggcggggata
     3301 ggacgggtta acgaactgcg catcggcttc aatgattacc tggcctttag ggcccggcag
     3361 cgcggtcttg attagcggcc ggcgcggcgg tgccatagtg gtgctcgaca tttgtttctc
     3421 tccttcttaa atcgcgcttt tgataagcgg tcgggaaaat tacaagtttc cctgaataaa
     3481 atcctgggga aataagggca gagccggttc aaagaaaggg actagtcggc gaagagattc
     3541 gcgcgggaat cgagcggcag ggtatgtagt gcatgcttaa acatcggttc ggattttaca
     3601 cgttccggtt gcggatgagc aatggagagg agtcgacacc cgcgagggcg cggcgcgaaa
     3661 gggaattggg ccacagattg acgcagagac acggctaaga accaatcatt ctgatccggg
     3721 taatccggtt aatttgtggc ttattccttt cgcgctccgc ggtcatttgg gagagacgtt
     3781 tgcaaaccag caggttcctg tggcagtcct tcgatgaaat ggtcgaaact gagccagata
     3841 ctccaactgt gatgaaaaaa cagaacggga aagatcagcg ccaaacccag caagacacca
     3901 atgacgagtt gataatgcgc agcaacgatt ggtagcgaga tcaggtatac caacacaatg
     3961 actgcttcgg tggtaacgac ggccaccaga atggcgccca caaaaaatcc ctcttcgcgt
     4021 ttgaagactg cgccgcacgc agcacaacgc tcttcgatct gaaaaagccg cgcgaagatc
     4081 cgcgatcggc cgcaaaccgg gcaacgaagt gctaaacacc aggccagcgc tttgctcaag
     4141 ggtggacgaa tgcgtttcac agttaagttc ctgctctaac cataaggaca tcaaaaacct
     4201 gtgaactctg aatgaggaag attgatacta ctatacggct ccggaattta aggtactcgc
     4261 taagctgatc gtaacgatta acccgccacc aatggcattc gccgcagtga ctgtcccccc
     4321 gtgaagctgg attgcgcggt cggctatggc caatcccagt cctacgccgc cggcttcgcg
     4381 atcgcgcgcg tcgtctacgc ggtagaaagg ccgaaagatc tctgtaagcg ctgcatcagg
     4441 aacgcctgga ccatgatcac gcaccgtgat ggtgagtact ctggcattag tctcggtcga
     4501 agtctctcgc acttctattt ctacttctgt tccttcggcc gtgtaatgga cggcgttgcg
     4561 cacgacgttc tcaatcgcgc gccgcagcag ttgctcgttg ccgttgaagg agcaggcagc
     4621 gacgctctcg agtttcacgg cgcgattgcc g
//
LOCUS       DASUEX010000242         2849 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1171734, whole genome shotgun sequence.
ACCESSION   DASUEX010000242 DASUEX010000000
VERSION     DASUEX010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2849
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1171734"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..673)
                     /locus_tag="VFV61_04095"
     CDS             complement(95..673)
                     /locus_tag="VFV61_04095"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HEX4899687.1"
                     /translation="MLEQKLILASKSPRRAELLVAAGWEFQAVAANVDESRHDGEAAV
                     AYVRRLAQTKAEKVAQQFPHDLVLGADTTVVVGAEILEQPGDAATAKWMLNLLSGKWH
                     QVLTGVALVRMSDSSLLVEHEVTQVRFAELSAAEIDWYVATGEPLDKAGAYGIQGRAA
                     PFIEEIQGDYFNIVGLPMRLVYNLIRKNFTRV"
     gene            1129..1578
                     /locus_tag="VFV61_04100"
     CDS             1129..1578
                     /locus_tag="VFV61_04100"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HEX4899688.1"
                     /translation="MDSLIKTLPAIMKAAGDSNEVAQAACFVAWKQIAGEGLRGNAVP
                     LCLDDRTLVIAVADAAWQKQMQSLSGQLLFRLNSLLGQPVVTFLEFREDPETVAQARI
                     ALTNVAEAREPAPVPAELVSAAAGIHDEDLRRAFLGAASSCIRRVEN"
     gene            1613..1909
                     /gene="gatC"
                     /locus_tag="VFV61_04105"
     CDS             1613..1909
                     /gene="gatC"
                     /locus_tag="VFV61_04105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEX4899689.1"
                     /translation="MQIKESDIEKIADLAHLEITPAEVAMFTPQMTEIVTYVEQLNDL
                     DTSSVEPALGGLTPAGEATDSARVDEVVPSLGQKLALAEAPDAAAGHFRVPKVL"
     gene            2027..>2849
                     /locus_tag="VFV61_04110"
     CDS             2027..>2849
                     /locus_tag="VFV61_04110"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEX4899690.1"
                     /translation="MSITELREQLISEQTTAEATVQASLKAAESLQPDLNAFLQIDRE
                     GARARAQSIDALPKDGESLPALAGVPVAIKDNICVRGLQTSCGSKILGDYHPPYDATV
                     IQRLQAAGAVIIGKTNCDEFAMGSSNENSAFGPVKNPWDVTRVPGGSSGGSAAAVAAG
                     IVPVALGSDTGGSVRQPASLCGVVGMKPTYGRNSRYGLVAFASSLDQVGIFGRSTRDV
                     AAVLGVIAGRDIHDATTAAVPVPDYVAELEAGGKNLRLGFPRALFGEGLDDDVRAA"
BASE COUNT          651 a          795 c          783 g          620 t
ORIGIN      
        1 tgctcgccgc ttgtttgttt ttcttagccc tgaagttacc cacgacgcag gttacggaat
       61 tggctcatga cttatgaacc aaagccaata acattcatac ccgcgtgaag tttttccgga
      121 tcaagttgta aaccaaccgc atcggtaacc ctacgatatt gaaatagtcg ccctgaatct
      181 cttcgatgaa gggcgcggca cgaccttgaa tcccataggc accggcttta tcgagtggct
      241 cgcctgtcgc cacataccaa tcaatctccg ccgccgaaag ttcggcaaag cgcacctgcg
      301 tgacttcatg ttcgacaagg aggctcgagt cactcatgcg cacgagggcc actccagtta
      361 acacctgatg ccatttcccg ctgaggaggt tcagcatcca cttcgcagtg gccgcatctc
      421 cgggctgttc cagaatttcc gcaccaacta cgacagtcgt atcagcgccg agcaccagat
      481 cgtgaggaaa ctgctgggcg actttctcgg ctttcgtctg cgccaagcgt cgcacatagg
      541 cgactgcagc ttcgccgtca tgacgactct cgtcgacgtt tgcggcgact gcttgaaact
      601 cccagcccgc cgcgaccaag agttccgcgc gccgcggact ctttgaagcg aggattagtt
      661 tctgttctaa cactggactt ctttcaatgt tgcacaggag gtggcggtga ttctagttcc
      721 gcctgctttg gtcgcaagaa cactaacaag acgcaataag taaggagggt gtaaaggaat
      781 acgttacagc caattacgat aagaagaagc agagtctcgt tgctcaaaac atctagcaga
      841 aacaagcggt aaagaaggag cacgggccat ctaaccggat agctcagata gtgattcagg
      901 gactcgtttt tgatgtaggt acttaagggt ccaacgatga tggcgtagac gaaaggtatg
      961 acgatcccgc cgagcagtga caacgccaaa cgtttcatgg cgcgattata agttaatcga
     1021 gaagcgcgac aatctcactt gatacctcgg cttgttggtt tttagaattc tccgctacac
     1081 tacgacgcta cttcccaata gaatctacgc acgccacttt tagttgaaat ggacagccta
     1141 atcaagacct tacccgcgat catgaaagcg gcgggggatt caaatgaggt cgcacaagcc
     1201 gcttgctttg tggcgtggaa gcagattgcc ggcgagggcc tgcgcggcaa tgccgtgccc
     1261 ttgtgtctcg atgatcggac gctggtaatc gcggtcgctg acgctgcctg gcaaaaacag
     1321 atgcagtcgt tgagcggaca gctcctgttc cgtcttaatt cactgctggg ccagccggtc
     1381 gtcacctttc tggagtttcg cgaggatccc gagacggttg cgcaagcgcg gattgcactt
     1441 acgaatgttg cggaagcgcg tgaacctgcg cccgttcctg cggagttagt atcggcggcc
     1501 gcgggaattc acgacgagga tttgcgccgc gcctttctcg gcgctgccag cagttgcatc
     1561 cgacgtgttg agaattagta accgcaaact gttaggttgc gattcatgaa tcatgcaaat
     1621 caaagaatca gacattgaaa agattgctga cctcgcgcac ctggaaatca ctcccgcgga
     1681 agtcgctatg tttactccac aaatgaccga gatcgttacg tacgttgaac aacttaacga
     1741 tctcgacacg agcagtgtcg aacctgcgct cggcggctta acaccggcag gcgaagcgac
     1801 agactcagca cgagttgatg aagtcgtacc ttcactcggc cagaagcttg cgctcgcaga
     1861 agcgcctgat gctgctgccg gccactttcg cgtgccgaag gtgctttaag agaggagtca
     1921 gtagtcagga ggaagtagac gtagcgacta ggcattccgc ggtagcggaa gttaatcgcc
     1981 aatcgccaat tggcaattgg caattggcaa tttagaaatc accatcgtgt ctatcacaga
     2041 actaagagag cagctcatta gtgagcaaac aaccgcggaa gcaacggtgc aagcatcact
     2101 gaaggcggcg gaatcgctgc aaccggactt gaacgcgttc ctgcaaatcg accgcgaagg
     2161 ggctcgtgct cgagcgcagt cgattgatgc gttgccaaag gacggtgaaa gtcttcccgc
     2221 cctggctgga gtgcccgtcg ccatcaaaga caacatctgt gtccgcggcc tgcaaacttc
     2281 ctgcggctca aaaatcctcg gcgactatca tccgccttac gacgccacgg tgatccaacg
     2341 gctgcaagcg gccggcgcgg tgattatcgg aaagacaaac tgcgatgagt ttgccatggg
     2401 ttcttcaaac gagaactctg ccttcggccc agtcaaaaac ccctgggacg tcacgcgcgt
     2461 gcccggtggc tcctcaggtg gttcggcggc cgccgtcgct gctggcattg tccccgtggc
     2521 cctcggttcc gacaccggag gctctgtacg ccagccagcc tcgttgtgcg gcgtcgtcgg
     2581 catgaaaccg acgtacggtc gcaactcacg ttacggcctg gtcgccttcg cttcgtccct
     2641 ggatcaggtg ggaatttttg gtcgcagtac tcgcgacgtg gcagccgtgc ttggcgtaat
     2701 cgcaggccgc gacatccacg atgccactac cgcagctgta cctgtgcctg attacgtcgc
     2761 tgaacttgaa gccggcggca aaaatctgcg gctgggtttt ccacgtgcac tctttggcga
     2821 aggtctcgac gacgacgtgc gtgcggccg
//
LOCUS       DASUEX010000243         2836 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_729450, whole genome shotgun sequence.
ACCESSION   DASUEX010000243 DASUEX010000000
VERSION     DASUEX010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2836
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_729450"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1355
                     /gene="uvrC"
                     /locus_tag="VFV61_04115"
     CDS             <1..1355
                     /gene="uvrC"
                     /locus_tag="VFV61_04115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEX4899691.1"
                     /translation="IKRCLGPCVKGLCTIDEYQEAVKDVRLLLEGRNKELADMLGDRM
                     AEASAEMRYEMAAKYRDLRKTVVRLSEQQKMATTSERDVDSFAYYREGRQLALQLFTM
                     REGNIVGRREFFWEDIDDDFNPASFLSDVLAQYYSSDYVPREIYVPVDFEDRELLEKA
                     LSERKGRRVRIRHSQRGEKRDMIDLGEKNAKLAFEQRFRVLRPDMKLVLEELQETLEL
                     PRFPARIESFDVSNIQGAENVAGMVVCENGKMNRAEYRKFKIRSVEGSNDVASMREAV
                     FRRYRRVLDEGRPLPDLIMIDGGKGQLNAAAEAMRELDLEAVALVGIVKPPLRHNEVS
                     HLLVKGREDEPVYLDSHSLILRLLQMIRDETHRYAVTYHRKRRELRDFTSELTAIPGV
                     GAKRKTRLLRHFGSIQRVSQASVSELSPFVGLKTAQEITDHFARQRALAAGGSAAEAS
                     "
     gene            1684..2175
                     /locus_tag="VFV61_04120"
     CDS             1684..2175
                     /locus_tag="VFV61_04120"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HEX4899692.1"
                     /translation="MASKASIAGHPVHPMIIPFPLALWTTSFITDVLFYFTRHGTLPV
                     IAKFLLAAGCIGAVAAAIPGIIDWLSIKHPATKSIANWHGRLNIIALLIFAASLYLRM
                     KSGGAAWVGYHLKIPFLLSLVGVILISISGWLGGALSFEHGVGVKPQHDTPEDEVAKV
                     RLP"
     gene            complement(2286..>2836)
                     /locus_tag="VFV61_04125"
     CDS             complement(2286..>2836)
                     /locus_tag="VFV61_04125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899693.1"
                     /translation="GNPSFHLAGGQPAAGDAIVSEAAFQQGCTNTITLAPAGSNVKTE
                     VQFTNGKVQAPLAFSGSYNLLGNCMQFKGWAQTSIALRFDQASQTVYGQVDVEGVNLE
                     GVNPIANNFVTVFVRNAIDQRVNPLELIRGQQLQLLIPVKASNGAVQAHVKDVRAEML
                     DGSLRMHISYEFTGAKGGQPQG"
BASE COUNT          603 a          776 c          795 g          662 t
ORIGIN      
        1 acatcaaacg ttgcctcgga ccgtgcgtca agggtctttg caccatcgat gagtatcagg
       61 aagctgtgaa agatgtgcgt ctcctgctcg aaggcagaaa caaagaactc gcggacatgc
      121 ttggcgaccg catggccgaa gcttcggccg agatgcgtta tgagatggcg gcgaagtatc
      181 gcgatttgcg caagaccgtc gtccgtctct ccgaacagca gaagatggct accacttccg
      241 aacgcgatgt tgatagcttc gcctactatc gcgaaggtcg gcagcttgcc ctgcaacttt
      301 ttaccatgcg cgaaggcaat atcgtcggca ggcgcgaatt cttctgggag gatatcgacg
      361 acgacttcaa tccagcgtca ttcctgagtg atgtcctcgc gcagtactac tccagcgact
      421 atgtaccgcg cgagatctac gtgcccgtag actttgaaga tcgcgaactg ctggaaaagg
      481 ccttgagcga gcgcaagggc cggcgcgtca gaatccgcca ttcgcagcgt ggcgaaaagc
      541 gcgacatgat cgatctgggc gaaaagaatg ccaagctggc attcgaacaa cgctttcgcg
      601 tgctgcgtcc cgatatgaag ctcgtgctcg aggagctgca ggagacgctc gagctgccgc
      661 ggttcccagc gcgcatcgaa tcgtttgatg tttccaatat tcagggagct gagaacgtgg
      721 ccggcatggt tgtttgcgaa aacggaaaaa tgaatcgcgc ggagtatcgc aagttcaaga
      781 ttaggagcgt ggaaggctcc aacgatgttg cctcaatgcg cgaagcagtc tttcgtcgct
      841 accgccgcgt gctcgatgaa ggaagacctt tgccggatct aatcatgatc gatggcggta
      901 aaggccaact taacgcggcc gccgaggcga tgcgcgaact cgatctggaa gcagttgccc
      961 tcgttgggat tgttaagccg cccctgcgac acaacgaggt ttcgcacctt ttggtgaaag
     1021 gacgagagga cgagccggtc tatctcgact cacattcgtt gatcctgcgc ttgctgcaga
     1081 tgattcgcga cgagacccat cgctatgcgg tgacttacca tcgcaagcgg cgcgagttgc
     1141 gcgatttcac ctctgagtta actgcaattc cgggagtagg ggccaaacgc aagacgcgtc
     1201 tgctgagaca ctttggatcg attcagcgag tttcgcaggc gagcgtttcg gagctatctc
     1261 cctttgtcgg cttgaaaaca gcgcaggaga tcactgatca cttcgcgcgg caacgggcgc
     1321 tggccgcggg cggctccgcg gctgaagcca gttgaaactc attcttcctg ggatttgcct
     1381 cagaattcag gcgctatgtt ggcttcggct ttgctgccgg gtgtgagcgg caagccgtag
     1441 ctgcatcacg ggctttttgg ggaactcacc tttcctggtt gttatttctc ctggtttggt
     1501 agatcgtacg accggcaaca acaagggaat ccttagcagt taaccggggc tgtcattttt
     1561 cgctcttcgc ttcgtcaatg acgcgcgaca gcaacctcgc gtttgcccca atcgaagtta
     1621 atccccacac ctgaggtagg agaatccgga tcggaatcag gtgctcttaa caggaggatt
     1681 gccatggcga gtaaagctag cattgctggt catccggtac acccgatgat cattcctttc
     1741 cccctcgcgc tctggacgac atcttttatc accgacgtac tattctattt cacgcgtcac
     1801 ggaaccctgc cagtgatcgc aaaatttctg ctggcggcgg gttgcatcgg cgcagttgcc
     1861 gctgccatac ctgggatcat tgattggctt tcgatcaagc accctgcaac caagtcgatc
     1921 gcaaactggc acgggcgctt gaacatcatc gcgttgctga ttttcgcggc gagtctttac
     1981 ctgcgaatga aatcgggcgg cgcggcatgg gtcggctacc acttgaagat tccgtttctg
     2041 ctttcgttgg taggagtgat cttgatttcg atttcaggtt ggctgggtgg ggccttgtcg
     2101 ttcgagcacg gcgtcggagt caaaccgcag cacgacacgc cggaagatga agtggcgaaa
     2161 gtgcggttgc cgtgatacct gagtccagtg tccaatgtcc aatgtccgtc ggcaaactta
     2221 ggcgcgaaaa ttcgaaactc gtctagcgga ccttggacgt tagacattgg gcgttggact
     2281 tgtttttacc cctgcggttg gccacctttc gctccggtga actcgtaaga gatgtgcatt
     2341 cgcaacgagc catcgagcat ttcagcgcgc acgtctttca cgtgcgcttg cactgcgccg
     2401 ttgctggcct tcaccggaat caacagttgc agttgctgcc cgcgaatcaa ctctaacgga
     2461 ttcaccctct gatcgattgc attgcgcaca aagaccgtga cgaagttgtt tgcaatcggg
     2521 ttcactccct caaggtttac gccctcaaca tcgacctgtc cataaactgt ctggctggct
     2581 tgatcgaagc gtagtgcaat gctggtctgc gcccaacctt tgaactgcat acagttgccc
     2641 aacaaattgt aactgccact aaaggccagg ggcgcctgca ccttgccatt ggtgaattgc
     2701 acttcagtct tcacattgct tcccgcaggg gcgagcgtaa tcgtattcgt gcacccctgc
     2761 tgaaacgccg cttcgctcac aatcgcgtcg ccggctgctg gctgaccacc agccagatga
     2821 aagctgggat tgccta
//
LOCUS       DASUEX010000244         2502 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1172517, whole genome shotgun sequence.
ACCESSION   DASUEX010000244 DASUEX010000000
VERSION     DASUEX010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1172517"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="VFV61_04130"
     CDS             complement(<1..392)
                     /locus_tag="VFV61_04130"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX4899694.1"
                     /translation="MKEPSTKVISFPSPVPSKADYQRVKSSYSIKEIKQLFGLSEKTL
                     RRWTEQGIIEASSTSEAPDYVYDFSALTQFRRVRELRAQGQSIRQIEAELQGQLNLFR
                     IETPRLARLLTPFEEALLLHEQGDTKAA"
     gene            complement(470..1354)
                     /locus_tag="VFV61_04135"
     CDS             complement(470..1354)
                     /locus_tag="VFV61_04135"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEX4899695.1"
                     /translation="MRSIWKGHIRFLLVAIPVRIYNAIETSEKIQFNQLHRDDFGPIG
                     YDKRCKKCNQVVTNDQITKGYQYEPDRYAIVEPDDISKIKIKTTKAVDIVGFVDREEI
                     PSTFYDAPYFAGPDGAVSEKPYALLREVMKQTGKIAVGKVVLRDREDLVAVFPHQDGL
                     VLQKLHYPHELRKMEDVPDLESVQKLDAQKLNKNELKLATTLVQEMTTSLSEIDTEDN
                     YYNALRTLIDSKIQGRDVVDFEPVEELPRLDIMSALKKSLQASSRKPMVKAPAPAAKK
                     KPQISLVKPRAAKKRKVG"
     gene            complement(1436..>2502)
                     /locus_tag="VFV61_04140"
     CDS             complement(1436..>2502)
                     /locus_tag="VFV61_04140"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX4899696.1"
                     /translation="NNGDRIQCGETILVVEVTAESIDLSETTQDAGMPARPVLVMVEC
                     VNCGRREQAQASAPDEHLTFLCEDCRIELKRSPQAIPGYDTVKLLGRGGMGCVMLGRD
                     QNTGRAVAIKTLLPEFAVSDKAMKRFMREIDVAAALKHKNIVEFIDRGTHKGVVYLIT
                     EYVDGADASKLAENNGGRLSYEDGISIISQALDALSFAHAQGYIHRDFKDQNILVSGS
                     SPNLIAKLTDFGLAKSFSNSGMSGVTMAGEMAGTLAYMPPEQLRNFRDVKPQSDIYAV
                     GMTAYSLLTGSLALDLTKSSGVNDTIRAIFEQPAVPLRQRAPHIPQPICEIIDRALAK
                     DPAQRWQSAGAMHNALLHSM"
BASE COUNT          521 a          687 c          676 g          618 t
ORIGIN      
        1 tcggctgctt tcgtgtcgcc ctgctcgtgt aatagcaggg cttcttcaaa tggggtgagt
       61 agacgtgcga gtcgcggcgt ctcgatgcga aacaggttca actgtccctg gagctcggct
      121 tcaatctgcc ggatgctttg accctgtgcg cgcagctcac gcacgcgacg aaactgagtg
      181 agggcgctga aatcgtagac gtagtcaggc gcctctgatg ttgacgaagc ctcgatgatg
      241 ccttgctccg tccaacggcg cagtgttttt tcactcagac caaagagttg ttttatttct
      301 ttgatggagt aagaggattt gacacgttgg taatctgcct ttgacgggac aggtgaggga
      361 aacgagatta ccttagtgct cggctctttc atttaaaact tgggacagga tttacaagat
      421 tgaagacaag atttacatgt caatcttgaa acaaatcctg tcaatcctgt catccaacct
      481 ttcgtttctt cgcggcacgt ggtttcacca gtgagatctg cggcttcttc ttggccgcgg
      541 gcgccggcgc ctttaccatc ggcttgcggc tcgaggcctg caaactcttt ttaagcgcgc
      601 tcatgatatc aagtctcgga agttcttcaa ccggctcaaa gtccacaaca tcgcgccctt
      661 gaatcttcga gtcgatcagc gttctgagcg cgttgtagta attgtcttca gtgtcgatct
      721 cggagagcga cgtcgtcatc tcctgcacga gggtcgtcgc cagtttcaac tcattcttgt
      781 tcaacttttg cgcatcgagt ttttggacgc tttcgagatc gggcacgtct tccatcttgc
      841 gcaactcgtg cgggtagtgg agcttctgaa gcaccaaacc atcctgatgc ggaaacacgg
      901 ccaccagatc ttcacgatct ctcagcacaa cctttccgac cgcaatcttg ccagtctgtt
      961 tcatcacttc acgcagtagc gcgtaaggct tctctgaaac cgcgccatcg ggtccggcaa
     1021 agtacggcgc gtcgtaaaac gtgctcggta tctcctcgcg atccacaaag ccgacaatgt
     1081 cgacagcctt cgtggtcttg atcttgatct tcgagatatc gtcaggttcg actatcgcat
     1141 aacgatccgg ctcatactgg tagcctttcg tgatctgatc gttggtgaca acctgattgc
     1201 acttcttgca gcgcttgtcg taaccaattg gaccaaagtc gtcgcggtgc agttggttga
     1261 actgaatctt ctcgctggtc tcgatggcgt tatagatgcg aacggggatc gcaaccagca
     1321 ggaatcgtat gtggcccttc cagattgatc tcatatcgac ctctcctcaa ggcggattat
     1381 gccacggatc tcttcgtttt ccacagattt atgtggaccg ccggggtgag cgtatttaca
     1441 tggagtgaag cagcgcgtta tgcatggcgc cggcgctttg ccagcgctgt gcaggatcct
     1501 ttgcaagggc gcgatcgata atttcacaaa ttggctgagg gatgtgaggc gcgcgctggc
     1561 gcagcgggac cgcgggctgt tcgaagatgg cgcgaattgt gtcgttgacg ccgctgctct
     1621 tggtcaagtc gagcgcaagc gagcctgtta acaggctgta ggccgtcatg cccaccgcgt
     1681 aaatgtctga ctgcggcttc acgtcgcgaa aattccggag ttgctcgggc ggcatatacg
     1741 cgagcgtacc agccatttcg ccggccatcg tgacgccact catgccggaa ttagaaaaac
     1801 tcttggcgag gccaaagtca gtcagcttcg cgatgaggtt tggcgaacta cccgatacga
     1861 gaatgttctg atccttgaag tcacgatgaa tgtagccctg tgcgtgcgcg aacgagagcg
     1921 catcgagcgc ctgggaaatg atcgagatac cgtcttcgta agaaagtcgc ccgccgttgt
     1981 tctcagcgag cttcgatgca tcagcgccgt cgacgtactc cgtgatgaga taaacgacgc
     2041 ctttgtgcgt gccgcgatcg ataaactcga cgatgttctt gtgcttcaac gccgccgcga
     2101 catcgatctc gcgcatgaag cgtttcatcg ctttgtcact tacagcaaac tcgggaagta
     2161 aagtcttgat cgcaactgcg cgacccgtgt tttggtcgcg cccgagcatc acgcaaccca
     2221 tgccgccgcg tccgagcagt ttcacagtgt catagccagg gatcgcctgc ggcgaacgct
     2281 tgagctcgat gcgacagtct tcgcaaagaa aggtcaggtg ctcgtccggc gctgaggcct
     2341 gtgcctgctc gcgacggccg cagttaacac actcaaccat cacgagcaca ggtcgcgccg
     2401 gcatacccgc gtcctgagtc gtctcagaca gatcaatcga ctcggctgtg acttcaacga
     2461 cgaggatcgt ttcaccacac tgtatccgat cgccgttatt ca
//
LOCUS       DASUEX010000245         4019 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1173112, whole genome shotgun sequence.
ACCESSION   DASUEX010000245 DASUEX010000000
VERSION     DASUEX010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4019
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1173112"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1349)
                     /locus_tag="VFV61_04145"
     CDS             complement(<1..1349)
                     /locus_tag="VFV61_04145"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEX4899697.1"
                     /translation="MAREQSSALKALERRLRRPLTKRERAAELRRIQGRYRRDRELAR
                     RRAEAARRAAIARQRAIEKAIRDEAQAFIARDDTAGEDPQVRRVALNALGNHAGTVVV
                     MDPMSGRIYTAVNQEWAYRRGFKPCSTIKLVTGLAGLNEKVIAPTDMEAVSDRYRIDL
                     TDALAYSNNTFFQQVSGKIGFEKMVSYARLLGLGQKTGVNAPNEYSGHLPLEKTNYSL
                     SRMGSHGDDFEVTAVQLANLVSAMGNGGKLLVPHIPKTPQEQAKFRAQVRDEINIEPE
                     YWRRMVPGMVGAVNYGSGKKAYDPLQTVAGKTGTCIGNGSWVGLFTSYAPLANPRLAV
                     VVIARGSDGRKHFPAAVAGQIYRELGSRFGTPVNLQIATAPADASEDSKAAELNEEEK
                     DAAAAEAEEMENVEKEAEAAGSAESTATAAPAKVPSAAAAPPQSKVKTVLMPIDRHNA
                     "
     gene            2016..3554
                     /locus_tag="VFV61_04150"
     CDS             2016..3554
                     /locus_tag="VFV61_04150"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX4899698.1"
                     /translation="MPRFVQSFRARLLFLLAVLLGLTLGVQYYVNLRSVSSNAHLIVE
                     QEQAIMAGVALGVNSLRSNKYLDQMKDSVKQPLLDEHSGRVTNILVVDSDGNVQDSLS
                     KAYSPQRRDDGSDVYVQLRDVPLPPLSSAVEMVDEGQPLPTWLAASKTGQAGEPGAFY
                     FPIETNKGRWYIIVVLGSAQTLKDILKQQASRSAFYTLVLLLITTLLTGYLVWRFTRP
                     IKDLSDAARRVAGGDFRYRVFSDRRDEMGSLASDFNEMTAKLERTRELESKLHQAEKA
                     AMVGRLAAAIAHEIRNPLNYINLTLDHLRSSFAPDDEPRREKFERLALQLKTEVGRIN
                     RHITDFLKYSRPSALELQPTNLRSEVEDVLRIISVQASECGINTHLEQKGEVPTVMAD
                     RESLRSMLTNLLINGMQAIDGQGGDLTVTLTTDSSASAQVEIADTGHGIAPEDISKVF
                     EPYYSTKETGTGLGLAIVKKAIDDHGGTIRVNSKLGSGTTFTITLPAIGKEQGESGGE
                     LAGQ"
     gene            3578..>4019
                     /locus_tag="VFV61_04155"
     CDS             3578..>4019
                     /locus_tag="VFV61_04155"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX4899699.1"
                     /translation="MARKSILVVDDEKPQREIYEAILSEAGYEVTTAASGEAALKFIN
                     ENKYDLVLTDLKMTGMTGIELLQHLTAHDSSISVILITGHGTIESAKEALRSGAFDYL
                     EKPVDREPLLEAVGRALSKRDELDMEIVSASPNMDSVKRMIVKVA"
BASE COUNT          916 a         1108 c         1076 g          919 t
ORIGIN      
        1 gcattatggc ggtctatcgg catcagcaca gttttgactt tgctctgcgg tggtgctgcg
       61 gccgcggatg gtacctttgc aggcgctgca gtggcggttg actctgctga ccctgctgct
      121 tcggcttcct tctcaacatt ctccatctct tcagcctctg ccgccgccgc atccttctct
      181 tcttcgttta gttccgccgc ctttgagtct tccgacgcat cggcaggggc ggtagctatt
      241 tgcaggttaa ccggagttcc aaagcggcta ccgagttctc gatagatttg accggcgact
      301 gctgccggga aatgtttgcg accatctgaa ccgcgcgcaa tcaccactac tgccagccgg
      361 ggattggcca gcggagcata ggaggtgaag agtcccaccc aactgccgtt gccgatgcag
      421 gtacctgttt tgccggcgac agtctgaagc gggtcgtaag ctttctttcc cgaaccgtaa
      481 ttgactgccc cgaccatgcc gggaaccatc cgccgccaat actcgggttc aatattgatc
      541 tcatcgcgga cttgggctct gaatttggcc tgttcctgcg gcgtcttggg gatatgaggc
      601 actagcagtt ttcccccatt gcccattgca gagacaaggt ttgcgagttg cactgcagtc
      661 acttcaaagt cgtcaccatg tgagcccatg cggctcagcg aataattggt cttctccagt
      721 ggaagatggc ctgagtactc gttcggcgcg ttgactccag ttttctggcc cagcccaagc
      781 agccttgcgt aactgaccat cttctcaaag ccaattttgc cgcttacttg ctggaaaaag
      841 gtgttattcg aataggccag cgcgtcagtc agatcgatac ggtaccggtc ggacacagct
      901 tccatgtcgg ttggtgcaat aactttctcg ttcaggccgg caagtcctgt gaccaacttg
      961 atagtggagc atggcttaaa tccacgccgg tatgcccact cctgattaac cgctgtgtag
     1021 attctaccgc tcatcggatc catcaccacg actgtgccgg catggttgcc tagcgcattc
     1081 aatgcgacgc gtcttacttg cggatcttca ccagcagtgt catctcgagc tatgaatgct
     1141 tgggcctcat cgcgaatggc tttttcgatt gcacgctggc gagcaatcgc ggcgcggcga
     1201 gcggcttcag ccctacgccg agccaactct ctgtcgcgac gataacgccc ttggattctc
     1261 ctcaactcag ccgcacgctc acgcttggtc aaaggtcgac gaagtcggcg ctcgagcgct
     1321 ttcaacgcag aactttgttc cctagccatc tgctgccgct gacggcgaag ttcacgcgca
     1381 gtcaactggc gtgtgcggcc gcgacggcta cgtcgcgccg agtgccggcc accccgtcgt
     1441 gaagaacggc gatcacgagc tgacgttcgt tttccttttt gagtgcgggc ggccttctga
     1501 cgtgaagcag aagcgcgcct ctttccgagg acagactcag agtagaaggg tagtataaga
     1561 agaacggaga gaattaaagc gaaacggata gtagtggggg aatgcttgat caaagtcatc
     1621 agggtcttta cttacctttc tcagggattt gctttgctca ttacggcatg agctcagcca
     1681 aaacagttcg gacgcgttcg ccggcaaccg gcacgtatcg cgcccttcct cctgggcaat
     1741 cgactttgca gccgcgagag ttttcaaaaa attcgggaca aatctctctt ctggctaatt
     1801 cctgcaacgt ttaggtggga gtgttcccta atggcggtcc ctggggacat tgatttgaga
     1861 ccgtcccaaa tataacatca ggcccagaaa agcggcaacc aaaaagtaat ctatcgtaca
     1921 tattccgaga atctctcgat tttgcacgtc tcccgaagac cacattcccc attacaacgc
     1981 ttttggtgta ctaatcttct tagtctgtga tgaagatgcc tcgttttgtt caaagctttc
     2041 gggcccgcct gctgtttttg ctggcggttt tgctggggct taccctgggt gtccaatact
     2101 acgtaaacct tcgttcagtc agtagcaatg ctcatctgat tgtcgagcag gagcaggcca
     2161 tcatggctgg tgtggccctg ggagttaaca gcctgcgcag caacaagtat ctggaccaga
     2221 tgaaggatag cgttaagcaa ccactgctcg atgaacattc ggggcgtgtc actaatattc
     2281 tcgtcgttga tagtgacggc aacgtccagg acagtttaag caaagcatat tctccacaac
     2341 gaagggacga tggttcagac gtctatgttc aactgcgcga cgttccttta ccaccactca
     2401 gcagtgcggt cgaaatggta gatgaaggcc agcccttacc gacgtggctg gccgcatcaa
     2461 aaacagggca ggccggagag ccaggcgcat tctactttcc aatcgaaacc aacaaaggcc
     2521 ggtggtacat catcgtggtg ctcggctcgg cgcaaacgtt gaaggatatc ctcaagcagc
     2581 aagcctcgcg ctcggctttc tacacgttgg tcctgctgct gattacaact cttctaaccg
     2641 gctacctggt ctggcggttc acacgaccga tcaaggacct ctcggacgcc gcgcgacgag
     2701 tggcgggtgg tgactttcgt taccgtgtct tctcagatcg ccgcgacgag atgggatcgc
     2761 tggcttcgga cttcaatgag atgacagcga agctcgaacg tacgcgggaa ctcgaatcga
     2821 aacttcatca ggctgagaag gcggcaatgg tcggaaggct ggcggcggcc atcgcacacg
     2881 agattagaaa cccgctcaat tacatcaatc tcactctcga tcacctgagg tcctcgtttg
     2941 ctccggacga tgaaccgcga cgggagaagt tcgaacggct ggcgctgcaa ttaaagaccg
     3001 aggttggcag aatcaatcgc cacatcacag actttctcaa atactcccga ccgtccgcgc
     3061 tggaactcca gcctacaaac ctgcgttccg aggtcgaaga tgtgttgcga ataatctccg
     3121 tgcaagcctc agaatgtggc atcaatactc atttggaaca aaagggtgag gtcccaaccg
     3181 tgatggcgga ccgcgagtct ttgcgctcga tgctgacgaa tctattgata aatggcatgc
     3241 aagctattga tggccagggc ggcgacctga cggttacgct aacgactgac agctcggcaa
     3301 gcgcccaggt agagattgcc gatactggcc acggcatcgc gcccgaggac atttccaagg
     3361 tatttgagcc ctattactcc acgaaagaga cggggaccgg gctgggactg gcaatcgtaa
     3421 aaaaggcgat cgacgatcac ggtggaacaa tacgcgtgaa ctcaaaactg ggcagcggca
     3481 caactttcac gattacatta ccggcaatag gaaaggaaca gggtgaatca ggcggcgaac
     3541 tggctggcca gtagtttagc gatcatccta atcacatatg gcgcgcaaaa gcatactggt
     3601 tgttgacgac gaaaaaccgc aacgtgaaat ctacgaggcg atcctatcgg aagccggata
     3661 cgaggtgacc accgcggctt ctggcgaagc tgcgctcaaa tttattaatg agaacaagta
     3721 cgacctggtg ctgaccgatc tgaaaatgac cggcatgacc ggtatcgaac tactgcaaca
     3781 cctgacggcc cacgactcct caatcagcgt gatcctaatc acaggtcacg gcaccatcga
     3841 atcagccaag gaagctcttc gcagcggtgc ttttgactac ctcgagaaac cggtagaccg
     3901 cgaacctttg ttggaagctg tcggccgcgc cttaagcaaa cgtgatgaac tcgacatgga
     3961 gatcgtctct gcttctccaa acatggacag cgtcaagcgg atgattgtga aagtcgctc
//
LOCUS       DASUEX010000246         3962 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1288318, whole genome shotgun sequence.
ACCESSION   DASUEX010000246 DASUEX010000000
VERSION     DASUEX010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3962
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1288318"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..582)
                     /locus_tag="VFV61_04160"
     CDS             complement(<1..582)
                     /locus_tag="VFV61_04160"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX4899700.1"
                     /translation="MTETADVVIIGSGIVGSSVAYHLAEQGCTNVLVIEREAHQGKGS
                     TGKSMGGVRAQFATPVNIQMSLYSIDFFSKFDDVIGYPADYRAHGYLFCATTERHLQY
                     LRANRERQLALGLKQVEEVNAAEIARFVPQLRVDDILGGTYCPTDGFVDPHSVMMGFM
                     LKARERGVRLWLDTQVTGIEVETATEAQRIAGVN"
     gene            674..1000
                     /locus_tag="VFV61_04165"
     CDS             674..1000
                     /locus_tag="VFV61_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899701.1"
                     /translation="MLHIFLSLLLVGLSLVISLRRYGVPIGYLTLSWVPLFLLFIASG
                     YQSLFGFKRFAEMIYHFSYDTSYILSLTGIVLLLTSIFRRTLTASLVLAVLLSALPIF
                     LLLLRS"
     gene            1006..2172
                     /locus_tag="VFV61_04170"
     CDS             1006..2172
                     /locus_tag="VFV61_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899702.1"
                     /translation="MLKTFDCPKCGGPVTYERQETPGFPDPKVVCNYCGSTLMVPDEL
                     HGQPARVVNIDLRSITRSKASRLFWVLLAIPVVIGLIVVLAMVGILAPVFYSINRAVT
                     PDTSKPTIARASRADEKPADSFASVLLKFGEEGIGPGMFKDARSIAVDGSGRIYVGEY
                     TGGRIQVFDAAGKFITQWNIGDRKTILRGLAADRKGTVYVVEGGKIHRYRGDAGTQLE
                     DLPYDDGWGFDDATVGADGSVIAAWYRNRDDIVRFSNSGQVTKTIRAAISGASGDSEL
                     NTRVAVDGLGNIYALGHFNSAVFKFTPEGKFLNQFGGQGEQPGQFRAPQAIAVDGKGR
                     VFVSDIKGIQIFDGNGRYLRTFSSEGGASGMVFNDNNELFVVGRKHVIKYSLKE"
     gene            2216..2707
                     /locus_tag="VFV61_04175"
     CDS             2216..2707
                     /locus_tag="VFV61_04175"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX4899703.1"
                     /translation="MNAEKYRRWFEYEEDSNAKVLTSLQAVAAPLRALPQFQKAVDLL
                     AHLIAARNMWLYRLGQGGQPPELFPAEASLNELPKLLDEMQSAWRSYLAALTDVEVAR
                     RFEYQSYDGPRFSNTVDEVLTQLYGHSLYHRGQIAMILRSIGAEPAVTDFIYWTREPV
                     PER"
     gene            2855..3514
                     /locus_tag="VFV61_04180"
     CDS             2855..3514
                     /locus_tag="VFV61_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899704.1"
                     /translation="MAATPSNELVRDNKWDTVERLARTLSIVAIPVVLAIGGWVIQQR
                     IQNQAVSRDYVQLAVTILKEPETSKVDPDMRAWAVQLLNDNSPTRFTPKFTEQLKSGE
                     TKLPEAFNISAAAAPAPIVSAGETKDDASSWEAKGFNALLNKDPQAALDAFSHAEKLW
                     PSFHSVAEIKRLLQDNQAALMAAPKEGNSEVWVNFYRTLVSKYSWGMPAEIKEQFNQQ
                     L"
     gene            3580..>3962
                     /locus_tag="VFV61_04185"
     CDS             3580..>3962
                     /locus_tag="VFV61_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899705.1"
                     /translation="MARSLLATLTVLLSLCSVAPAQASLAAFRYQPGKIVTGVVYHYL
                     KTNIDGTNPEHISIRIAAKDRIESFKFHPQGTRAGLVIAQMDWQIFSVKRLESWQVYK
                     DKQKVLAATLVYLPASKEVAVTLPF"
BASE COUNT          906 a         1033 c         1073 g          950 t
ORIGIN      
        1 attcacgccc gcgattcgct gagcttcggt agctgtctcc acttcaattc cagttacttg
       61 cgtatccagc cacaacctca cgcctcgctc gcgcgccttc aacataaatc ccatcatgac
      121 actgtgcgga tccacgaagc cgtcggtcgg acagtaggta ccacccaaaa tgtcgtcgac
      181 acgaagttgc ggcacgaaac gagctatttc cgcagcattt acttcctcaa cctgcttcag
      241 gccgagcgct aactgtcgct cgcgatttgc tcggagatat tggagatgtc tttccgtagt
      301 ggcgcagaac aagtagccat gcgcgcgata gtcagccgga tatccaatca cgtcgtcgaa
      361 ttttgaaaag aagtcgatcg agtaaagcga catttggata ttcacaggtg tcgcaaactg
      421 cgcgcgtacg ccacccatgc tttttccagt tgagcctttg ccctggtgtg cctcgcgttc
      481 gatcacgagc acatttgtgc aaccctgttc ggcaaggtgg taggccacgc tcgaaccgac
      541 aatgccgctg ccgatgatta cgacgtctgc ggtttcagtc atggttgtgg ttgcgggagt
      601 tggactctag aatgtctcgg cactttgctt caattaggtt acgcgtgttt aacccgtcgc
      661 tagtcctaaa acagtgctac atattttctt aagcctgctg ctagtcggcc tgtctctggt
      721 gatttcgctg cggcgatatg gagtcccaat tggctatctg acattgtcat gggtgccgct
      781 gttcctgctt ttcatcgcca gcggctacca aagtctgttt ggcttcaagc gcttcgccga
      841 aatgatctac cacttttcgt atgataccag ctacattctc tctctgactg ggatcgtgtt
      901 attactgact tcgattttta gacgaacgct cacggcgtcg ctggtgttag cagtcttact
      961 ttctgcacta ccaatctttc ttttattgct tcgcagttag aaaccatgct caaaactttt
     1021 gattgtccca agtgtggcgg ccccgttacc tacgagcgcc aagaaacgcc tggctttccg
     1081 gatccaaagg tagtctgtaa ttactgtggc agcaccttga tggttcctga cgagcttcat
     1141 gggcaaccag cacgcgttgt taacatcgat ctgcgatcca ttacacgcag taaggcttcg
     1201 cgcctgtttt gggtgctgct cgcaataccg gttgtaattg gtttgatcgt tgtgctggcg
     1261 atggttggga ttctggcacc cgtcttctat tcaatcaatc gcgccgtgac cccggatacc
     1321 agcaaaccga cgattgctcg cgcttcgcgt gcagatgaaa aaccggccga tagctttgct
     1381 agtgtgctac taaaatttgg cgaagaggga atcggcccgg gaatgttcaa ggatgctcgc
     1441 agcatcgcgg tcgatggctc tgggcgaatc tacgtgggcg agtacaccgg tgggcgcatt
     1501 caggtctttg atgcggcagg gaaattcatc acgcagtgga acatcggcga ccggaagaca
     1561 atcctgcgtg gcctggcggc tgatcggaag ggaacagtct acgtcgtcga aggcgggaag
     1621 attcatcgct atcgaggcga cgcgggaact cagcttgaag acttgccgta tgacgacggg
     1681 tggggatttg acgatgcgac ggttggcgcc gatggcagcg tcattgcagc gtggtatcgc
     1741 aatcgcgacg atattgtgcg ctttagcaac agcgggcaag ttactaagac aattcgcgcg
     1801 gcgatcagcg gcgccagcgg tgattcagaa ctcaacacgc gcgtcgcggt cgatggcctg
     1861 ggcaacattt acgctttggg tcacttcaac agtgcagttt ttaagttcac gccggaaggg
     1921 aagttcttga atcaattcgg tgggcagggc gaacagccgg gacaatttcg cgcgccgcaa
     1981 gcgatcgctg tcgatggaaa gggaagggtc ttcgtcagcg atattaaagg aattcaaatt
     2041 tttgacggga acggccgtta cctgcggacg ttttcatccg agggcggcgc ttcggggatg
     2101 gtattcaacg ataacaatga actttttgtg gttggacgga agcatgtgat caaatactcg
     2161 ctgaaggaat agtcaggtca accgaattgc agctgcggca gcaaggctaa taactatgaa
     2221 cgcggaaaaa tatcgacgct ggttcgagta tgaggaggac agcaatgcga aggtgttgac
     2281 ctcgctgcaa gcggttgctg ctccgctgcg ggccctgcct cagtttcaaa aagcagtcga
     2341 cctcctggca catctgattg ccgcgcgcaa catgtggctt taccggctgg gtcagggagg
     2401 tcaacctccc gaactcttcc cggctgaagc gtccctcaac gagctaccaa agctgttgga
     2461 tgaaatgcag tctgcgtggc gatcctacct cgcggctctg accgacgtgg aggttgcacg
     2521 acgtttcgag tatcaaagct acgacgggcc gcgctttagc aatacggtcg atgaagtact
     2581 gacccagtta tatggccact cgctttatca ccggggacag atagccatga tccttcgctc
     2641 aatcggcgcc gaaccggcgg tcacggattt tatctactgg actcgcgaac ctgtgccgga
     2701 gcgttgagag gtcattctca atgagtcagg tctccagatg cattcggctg agcaacttga
     2761 ggactccaaa ctctcttcac ccttggcggg attcagatag ccgaacagac ttcctttccc
     2821 accgaccaaa ctcttttcct ggaggaggct aacaatggca gctacaccct caaacgaact
     2881 cgtccgtgat aacaaatggg ataccgtcga gaggctcgcg aggacccttt ctattgtcgc
     2941 cattcctgtg gtgctggcga tcggcgggtg ggtaatccaa caacgaatcc agaatcaggc
     3001 cgtcagccga gattacgtcc agttagcagt tacgattctg aaggaacctg agacctctaa
     3061 agttgatccc gacatgagag cctgggccgt tcaattgctg aacgacaact cacccacaag
     3121 gttcactccc aaatttacag agcagttgaa atccggggag acaaaactgc cggaggcttt
     3181 caatatttct gctgctgcgg ccccggcgcc tatcgttagc gcaggtgaga ctaaagatga
     3241 tgcgagcagt tgggaggcca aaggatttaa tgctctgcta aataaagacc cgcaggcggc
     3301 cctggatgct ttcagccatg ccgagaaact ctggccttcg tttcacagcg tggccgagat
     3361 taagagacta ttgcaggaca atcaggctgc actgatggcc gcacccaagg agggcaatag
     3421 cgaagtgtgg gtaaattttt accgtaccct ggttagtaaa tactcctggg gcatgccggc
     3481 tgaaataaaa gaacaattca accagcagct gtgaagatag cgccccgggt gattgcggaa
     3541 gttagtcgcg atctactcat ttcaaattga ggtaaatcca tggcaagaag cttactggcg
     3601 actctaaccg tgctcttgtc actttgcagc gtggcgcctg cgcaggcatc gcttgcagct
     3661 ttccgttatc agcctggcaa gatcgtcaca ggtgttgtct atcactactt aaagaccaat
     3721 attgacggca ccaatccgga acacatctcg attcgcatcg ccgccaaaga tcgcattgag
     3781 tctttcaagt ttcatcccca gggaacgcgg gcgggattag tgatcgcgca gatggactgg
     3841 caaatttttt ccgtgaagcg tctcgagtcg tggcaggtct ataaggacaa acagaaggtg
     3901 ctcgcagcca cactcgttta tcttccggcg agcaaagagg tagccgtcac gctgccgttc
     3961 at
//
LOCUS       DASUEX010000247         5774 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1635710, whole genome shotgun sequence.
ACCESSION   DASUEX010000247 DASUEX010000000
VERSION     DASUEX010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5774
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1635710"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..791
                     /gene="xrtK"
                     /locus_tag="VFV61_04190"
     CDS             <1..791
                     /gene="xrtK"
                     /locus_tag="VFV61_04190"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR04287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="exosortase K"
                     /protein_id="HEX4899706.1"
                     /translation="RTSYCALVQLVVVLLCAVAVKQYYSTTSVNQLRWILAPTTWLVQ
                     FISGRHFEFEAYTGYMSSDRTFVIAASCAGVNFLITSFLMLSLRKLWRDRARQIPWRF
                     FAGMAAVAYVVTIVANSVRISSSLLMQRTPVEIGLSANQLHRFEGIFIYFGFLLLLFL
                     VSETISQRGTVATTGIRSAAGGLLRQALFPLLTYYATTLAIPMANGAYRQGSDFLEHA
                     LFVLVTPLLVMLPALVFVGLSSQSKRGSAPTSANHATINLMTEL"
     gene            885..1463
                     /locus_tag="VFV61_04195"
     CDS             885..1463
                     /locus_tag="VFV61_04195"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX4899707.1"
                     /translation="MARKGSNLNLPGALHFVTGNFLDRRQVFRDPACCEVFLEQLAMM
                     VRDWPSKLIAYVLMPDHFHLILNPRDGRIREFMLDLKSLSAKHIVQAKTRFGFAKTDE
                     DGFQVWQESFKALPLWSAWMIWQKINYTHANPVKAGLVKSARDYYWSSFGSFYSVGNT
                     PLAVDRDWWWPDDAEKLLEAMKKLGWYSYHRR"
     gene            complement(1479..4412)
                     /locus_tag="VFV61_04200"
     CDS             complement(1479..4412)
                     /locus_tag="VFV61_04200"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HEX4899708.1"
                     /translation="MTLFHGLPSDRVRAIAQGIDGSMWFGTEGGLAKFDGRRTQAIKD
                     PALPGNRILSLKTDDSGALWVGTDSGAARIIGSRVESIGDTLGNAISAIVIPEPGRLL
                     LATEQGKIFECRTTPSGAVQVQSLLPSPLESADRDNPGPLVITSLVSLQEWILAGSLS
                     RGVLGLRNGSVNEPSWNRAQFFIRALALDKHGTLWIGSRTKKQEPGLFTIGYRTFSEL
                     GDPPKRNNAPTGTVLTIQPIGAAMFVGTDGNGVFRFEDNKTERFTFEGTAGGLRSDRI
                     YAIFADREGVIWFGTDRGVSRFDPNAPRVEAVGTSPDNNFVRTIFRSSSGHLFAGTNR
                     GLFVYEERLRSWNPVQALDRSIIYALAEDSSGNLLVGSATGFYTSQRRINDGAAESLT
                     FTRVTTGSGGVDAQGGVRAIAQFRGQTYIASFGRGVEQYQGGRAKNVWPREAPSEVLS
                     LHAEGDSQLLVGTAQDGVLFFDGQQSIADPALANFKGVAIRAMTRTSDGTLWLATGRG
                     LYFCSGNNPCAAAAAGNDARALAVSLNANLNQVWCATGGAGLLRVSLDKDVGPVVSQL
                     DSEQGLPSQNAFAVLPPDDDALVLIGTNRGVVRYNPGHTVPTLFATRIIGKRVHQPAE
                     LAAGLNLEYPQNSLLLDVAAISSRTFPEQFQYAFVLRDAAGKTIKQRLSRESQFVLDG
                     LQPGRYRVMARAFTKDLIQSEPLIFEFSVAKAPFPWTSTALAILLALTLLALLWALLE
                     RRRIVRTSAALVTANHELADARLNLANEAERERHRIARDLHDQTLADLRHLLVLTDQL
                     PLKGNVNNQPVDASALRGEIEDISQEVRRICEDLSPSVLQNVGFSAALEFAMEQAVQH
                     APLERKFTYEFVCDDGLEEQINLPANVQMQIYRITQEAVNNLCRHAAPRHVWMKVSAA
                     ATGTFDLRLEDDGREFDPGTVQNSEGRGLANMQARAKLIDAEISWTRREGGGTVFSLR
                     RATKI"
     gene            complement(4908..>5774)
                     /locus_tag="VFV61_04205"
     CDS             complement(4908..>5774)
                     /locus_tag="VFV61_04205"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX4899709.1"
                     /translation="QAKQKSRLAEAFDWIIKLMSILIAEDNVAQRHYLRGILEKEFPS
                     HAPLLEASDGEETVRLALESRPEVCILDIQMPKLSGVKAARAIWNEFPKARIIFWTQF
                     PHEIYINEIRKIVKSVDPPPAYGFIHKNNPESRFLRFVASVLEDGADMIDPAFKDSFK
                     RPLLTEFEAEALYYLALGLSNWAIARKCSLSLRGVESRLATLYEKLFVSAAEGTEHET
                     YDKLAYNIRTRAFCEALRRGLLNNDELEKAAADLEGWTERDRARFIEEERTARRGDGE
                     TRRRGELETRKR"
BASE COUNT         1235 a         1622 c         1580 g         1337 t
ORIGIN      
        1 ggagaactag ttattgtgcg ctggtgcaat tagttgttgt gttgctctgc gcagtggcgg
       61 ttaagcagta ctactcaacg acgagcgtga atcagttgcg ctggatcctg gcaccgacga
      121 cctggttggt gcagttcatc agcgggagac actttgaatt cgaagcctac actggctaca
      181 tgagcagcga tcggaccttc gtgattgcgg cttcctgtgc cggagtcaat tttctgatca
      241 cttcattctt aatgctgtca ttgaggaagc tttggagaga tcgagcaaga caaatcccgt
      301 ggcgcttctt tgctgggatg gccgcggtgg cgtatgtggt cacgatcgtt gccaactccg
      361 tgcgcatttc ctcttctttg ctgatgcagc gaacgcctgt ggagatcggt ttgagcgcga
      421 atcaattgca ccgcttcgaa ggaatcttca tctatttcgg atttttgcta ctgctgtttc
      481 tggtgagcga aacgattagt cagcgcggga ccgtggcgac aaccgggatt cgttcagccg
      541 ccggcggtct cttgcggcaa gctctctttc cattgcttac ctattacgca acaacgctgg
      601 cgataccaat ggccaacggt gcttatcgtc agggatcgga tttccttgaa cacgcattgt
      661 tcgtgttagt gacgccgttg ctggtgatgt tgccggcgct tgtatttgtc ggacttagct
      721 cacagtcaaa aagggggtct gctcccactt ccgcgaatca cgcaaccatt aacctcatga
      781 ctgaactcta gcaatctcgc agttgggaag gaggcctacc cccgctcgtc taacaattat
      841 ccccttgcta gactgcaggg aaacctcaca aaggagctcc tcacatggct cgcaaagggt
      901 ctaacctcaa ccttcccggt gctctgcatt ttgtcacagg caacttctta gatcgtcggc
      961 aggtctttag ggatccggct tgttgtgaag tctttctgga acaacttgca atgatggttc
     1021 gcgattggcc aagcaaactg attgcatacg tcttaatgcc agaccatttt catctgatcc
     1081 tgaacccgcg tgatggtcgc attcgcgaat tcatgcttga cctcaaaagc ctgtcggcaa
     1141 aacatatcgt gcaggcaaag acgcgattcg gatttgcaaa aactgacgaa gatggctttc
     1201 aagtgtggca agagagcttc aaagccttac cgctgtggag tgcctggatg atttggcaga
     1261 agatcaatta cacccatgca aacccggtta aagccgggtt agtaaagtca gctcgcgatt
     1321 attactggtc aagctttggc tcattctact cagtcggaaa cactcctctt gcggtggatc
     1381 gggattggtg gtggccggat gacgcagaga agctcttaga agcgatgaag aaactcggtt
     1441 ggtacagtta tcataggaga tgacctcaag tccccgaact agatctttgt ggcccgcctc
     1501 aagctaaaca ccgtgccgcc gccctccctc ctggtccagg agatctccgc atcgatcaac
     1561 ttcgcacggg cctgcatgtt tgcgaggccg cggccttcgg agttctgcac cgtaccagga
     1621 tcaaattcac gtccatcgtc ctcaagtctc aaatcgaatg tacctgttgc cgccgccgac
     1681 actttcatcc agacgtgccg cggagcagcg tggcggcaaa ggttattcac tgcctcctgg
     1741 gtgattcggt aaatctgcat ctgcacgttc gcgggcaggt ttatttgttc ctcgaggccg
     1801 tcgtcgcaga cgaactcata tgtaaacttc ctttcgagcg gggcgtgttg cacggcctgc
     1861 tccatggcaa actccagcgc cgccgaaaaa cccacgttct gcaaaaccga ggggcttagg
     1921 tcctcacaaa tccgtcgcac ttcctgcgag atgtcttcta tctcgccgcg caaagcgctg
     1981 gcatcaaccg gttgattatt tacattcccc ttgagtggca gttgatcggt gagtacgagc
     2041 aaatggcgca ggtcggcgag cgtttggtcg tgcagatcgc gtgcgatgcg gtgccgttcg
     2101 cgctccgctt cgttggccag gttcaaccgc gcatctgcca gctcatgatt ggctgttacg
     2161 agtgccgcgc ttgtgcgcac gattcgtctc ctttcaagca aagcccagag cagcgccagg
     2221 agcgttaaag ccagcaggat agccagggcc gttgatgtcc aggggaaagg agccttagcg
     2281 acactgaatt cgaatattaa gggctcggat tgaatcagat cctttgtgaa cgcccgcgcc
     2341 atcacacgat atctgccggg ctgcaagcca tcgagaacga attgcgactc gcgtgacagt
     2401 cgttgcttga ttgtttttcc cgcggcatcc ctgagcacga aggcgtactg gaattgttcc
     2461 gggaaggtgc gactactgat tgccgccacg tccagcaaca gactgttttg cggatattca
     2521 agattcaaac cggcggcaag ttccgcaggt tgatggactc gtttaccgat tatgcgcgtc
     2581 gcgaagagcg tcggcactgt atggccaggg ttatatctga ctacgccgcg atttgtgccg
     2641 atcagtacca gcgcatcatc gtctggcggc aaaacggcaa aagcgttttg cgacggcagt
     2701 ccctgctcgg aatccagttg tgagacaacg ggcccgacat ctttgtcgag cgagactctt
     2761 agcaggcctg cgcccccggt tgcgcaccag acttgattca gatttgcgtt gagactaacg
     2821 gcaagcgcgc gcgcgtcgtt acctgccgcg gccgcggcac aaggattatt gcccgagcag
     2881 aaatacagcc cgcgaccggt tgccagccac agagtgccat cgctggttcg cgtcatagcg
     2941 cgaatggcaa cgcctttgaa attagccagt gccggatcag caatgctctg ttggccatcg
     3001 aagaagagca ctccatcctg tgcggtgcca acaagtaatt gtgaatcgcc ttccgcgtgc
     3061 agactcaaca cttctgacgg tgcttcacgg ggccaaacat ttttggcgcg tccaccctga
     3121 tactgttcaa cgccgcgacc aaaactggcg atgtatgttt gcccgcgaaa ttgagcaatc
     3181 gcccgcacac ccccctgagc gtccacacct cccgagccgg ttgtgactcg cgtgaaggtt
     3241 agcgattcgg ccgcaccatc attgatccgc cgttggctgg tatagaaccc ggtagccgaa
     3301 cctaccaaca gatttcccga actgtcttcc gccagagcgt agatgatgct gcgatcgaga
     3361 gcttgaaccg gattccaact tcgcaatctc tcctcataaa caaagagacc gcggttggta
     3421 ccagcgaaca ggtggccgct tgacgaccgg aaaattgtgc gtacgaaatt gttgtccgga
     3481 gaagtgccta cagcttcgac acgtggagcg ttaggatcaa aacgcgaaac gccgcgatcg
     3541 gtgccaaacc aaatcactcc ttcccgatcg gcgaagattg cataaatgcg atccgaacga
     3601 agcccgccgg ccgtaccctc gaaagtgaag cgttcggtct tgttatcttc aaaacgaaag
     3661 acaccattgc cgtctgtccc cacaaacatg gccgcgccga taggttgaat ggtaagcact
     3721 gtgcctgtcg gcgcgttgtt tcgttttggt gggtcgccga gttcactgaa cgttctgtaa
     3781 ccgatcgtga acagacctgg ttcctgtttc ttcgtgcgcg agccaatcca gagagtgccg
     3841 tgtttgtcca atgctaatgc gcgaatgaag aattgggcac ggttccagga cggttcgttg
     3901 acactgccgt ttctcaaacc aagtacgccg cgacttaaag agccggcgag gatccactct
     3961 tgaagtgaca cgagactggt gattactaac gggcccgggt tgtcgcggtc tgcgctttca
     4021 agcggggacg gcaaaagaga ctgaacctgc accgcgccgg acggagttgt gcgacattcg
     4081 aaaatctttc cctgttctgt tgccagcagc agacgcccgg gttcaggaat aacaatcgca
     4141 ctgatcgcat ttccgagtgt gtctccaatg ctttcgactc tacttccgat gatccgagcg
     4201 gcgccggagt ccgttcccac ccacagcgca ccgctatcat cagtcttgag cgaaaggatg
     4261 cggttgccgg gtagcgccgg atctttgatg gcctgggtgc ggcggccatc gaacttcgcg
     4321 agtcctcctt cagtgccaaa ccacatcgag ccatcgatgc cttgtgcgat ggcgcgcacg
     4381 cgatccgagg gcaagccgtg aaacaaagtc acggcgcccc actgatgcaa tcccgtaacg
     4441 ggcgtaggcg atggtgacgg cgagggcgaa ggagacggcg agacggatgg gttgggggta
     4501 ggagaaggtg aaggagtttc agtttgagtt tgtgctaagg agaaagctgg tggcagaaga
     4561 cagaaggcag caagcagaaa gcagcagacc gctgacaggg tgagaagcgg ggatggggag
     4621 acggggcgat taggtgacat cgttactaat gccttctgcg agaactgctt acttatgtcc
     4681 ttttgcgttc gcgtgtgtgc tcgtcttccc ggcgtcccgc gtcaccgtgt ctccgcgtca
     4741 ccgcatctcc ccgtcacatg tcaccgtgtc cccgcgtcac cgcgtcaccg cgtcaccgcg
     4801 tcaccgcatc tccccgtcac cgcgtccctc cgtcaccgcg tctcggtgtc cccgcgtcac
     4861 cgtgtccccg cgtcaccgcg tccccggctc gctacgactc gcgggcctca ccgcttcctc
     4921 gtctctagct cgccccgtct ccgtgtctcc ccatccccgc gtctcgccgt tcgttcctct
     4981 tcaataaacc gcgcacgatc ccgttcagtc caaccttcca aatcagcggc cgccttttcc
     5041 agttcgtcgt tatttagcag accacgccta agcgcttcac agaaggcacg cgtgcggatg
     5101 ttataggcga gcttgtcgta cgtttcatgc tcagtgccct cggctgcgga aacgaacagt
     5161 ttttcgtaga gcgttgctaa acgactctcc actccgcgca gactcaacga acacttccgg
     5221 gctatggccc agttggaaag accgagggcg aggtagtaga gcgcctcagc ttcaaactcc
     5281 gtcagcagcg gtcgcttgaa cgagtctttg aaggcagggt caatcatgtc agcgccgtct
     5341 tcaagcacgg aagcaacgaa acgcaaaaag cgcgactcag gattgttctt gtgaataaag
     5401 ccgtaagctg ggggtggatc taccgactta acgatcttgc gaatctcgtt gatgtagatc
     5461 tcgtgcggaa actgcgtcca gaagatgatg cgtgctttag gaaactcgtt ccagatcgcg
     5521 cgtgctgctt tcacgcccga gagcttcggc atttgaatgt cgagaatgca gacttccggt
     5581 ctgctctcga gagccagtct cacagtctcc tcgccatcag aagcttcaag cagaggcgca
     5641 tgtgaaggaa actctttctc aagaattccg cggaggtaat gtcgctgggc gacgttgtct
     5701 tcagcaatca ggatggacat tagcttgatt atccagtcaa aggcttcagc gagtctactc
     5761 ttctgtttcg cttg
//
LOCUS       DASUEX010000248         2141 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1635732, whole genome shotgun sequence.
ACCESSION   DASUEX010000248 DASUEX010000000
VERSION     DASUEX010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1635732"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(31..519)
                     /locus_tag="VFV61_04210"
     CDS             complement(31..519)
                     /locus_tag="VFV61_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEX4899710.1"
                     /translation="MLMSRRANGFSLIELLIVVAIIGIVASIAIPSLLASRRAANEGS
                     AQSSLRTIQSCQLTYQATVGRGDYGTLAQLRGEVLTDAVLGGGSKSGYNFAATPSAAG
                     VRPALFYATAVPIIPGGIGQTGTRRFGITEDGVIHGDTGGGLAAYANAVAIRAAPAMG
                     NQ"
     gene            complement(623..1111)
                     /locus_tag="VFV61_04215"
     CDS             complement(623..1111)
                     /locus_tag="VFV61_04215"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX4899711.1"
                     /translation="MKNNRQAGFSLIELLIVVAIIGIIAAIAIPNLLASRRAANEGSA
                     QSSLRTIHSAEGTYQSTVGSGNYGTLAQLGAQDLVDPVLRTGGKSGYNFAITPTPLVA
                     GVSPATFFATAVPQVVGGIPATGTRRFGISTDGVMHGDALGGLAAYADEAAVVAAPAL
                     GN"
     gene            complement(1417..>2141)
                     /locus_tag="VFV61_04220"
     CDS             complement(1417..>2141)
                     /locus_tag="VFV61_04220"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HEX4899712.1"
                     /translation="SFLLVDLSALLAVLPTTDGNELANSPRLLKFLSLIQPTLLLALA
                     VLVGVLLAPKVSLSAPAFEALAHRGNFIAALKPQIIPGVIAGLIGAVVIVLSWALFRS
                     FLPAEFVARAEAFNRLVPLPTRILYGGITEELLLRWGLLTLLVWAAWRVFQRGRRQPG
                     SVCFVGAILISSIVFGVGHLPIAVAIGGQLTLPVAVYIITANSVFGLIAGYLYWRKGL
                     EAAITAHMLAHVVIWSALYLGS"
BASE COUNT          499 a          609 c          623 g          410 t
ORIGIN      
        1 agggcgtgga agtattgaat gcgcggcgaa ctactgattt cccatcgccg gtgcagccct
       61 gatggcgacg gcgttcgcgt aagcagccag gccaccaccg gtgtcgccat ggatcacgcc
      121 atcctcggtg atcccgaaac ggcgcgttcc cgtctggcca atgcccccgg ggattatggg
      181 cactgcggtc gcgtagaaaa gtgccggtct cacgccggca gcactgggcg ttgccgcgaa
      241 gttgtatccg cttttagaac caccgcccag cacggcgtcg gttaaaacct ctccgcgcaa
      301 ctgggccagt gtgccatagt cgccgcgtcc cacggttgcc tggtacgtaa gctggcagct
      361 ctgaatcgtt ctcagcgatg attgagctga cccctcattc gccgcgcgac gggaagccaa
      421 caggctgggt atcgcaatgg atgccacgat tccgatgatc gcgacaacga ttagaagttc
      481 gattagtgag aatccgtttg ctcggcggct cataagcact accccctctc acgatttttg
      541 ggcgtgaggc gaatgggttg aaaaaaaagc ggggtggaag attctcccac cccgcctcca
      601 aggttttgat ctaagtcaaa ggttagttgc cgagggcagg tgcagcgacg acagcagctt
      661 cgtcggcata cgccgccaaa ccgccaagcg catcaccgtg cattacgcca tcggtcgaaa
      721 tgccgaagcg gcgcgtaccg gtcgccggaa taccgccgac aacctgcgga accgcagtcg
      781 cgaaaaacgt ggccgggctc acacccgcaa ccaggggagt cggggtgatg gcaaagttat
      841 aaccactctt gccaccggtt ctcagcactg ggtcaaccaa atcctgggcg cccaactggg
      901 ccagcgttcc gtagttgccg gaaccgactg ttgactggta ggtaccctca gcactgtgaa
      961 tggttcgcag cgaagactgg gcagagcctt cgttcgcggc gcgccgggaa gcgagaaggt
     1021 tcgggatcgc gatggcggcg ataatgccga taatcgcgac gacgataagc agctcgatta
     1081 acgagaagcc tgcttgacgg ttgttcttca tatctttctt ttgctcctta atgagataag
     1141 ctggtttttt gttgccttcg cgccaccggc tttgtctcgt cgatgaaccg cagcgcgcga
     1201 tttgtcgcgg tcgtactctt caaggcctgt gccatggcgc tcaagatcag aagcgcagaa
     1261 aaaagctgag gaaattcgcg aagttggtgt tgcccggaag aatgggccgg cgagaattat
     1321 gtgtggctga caattactgt cagcgagcca gtgacggttt tggtcagaga cgaaggatgg
     1381 attaagagaa ttgatccagt tacgctctca gaagttttag ctcccaaggt acagcgcgga
     1441 ccatattaca acgtgggcca acatgtgcgc cgtaatcgcg gcttccaatc ctttgcgcca
     1501 atacagataa ccggcgatta acccaaacac cgagttggct gtgatgatgt aaacagcgac
     1561 gggcaatgta agctggccgc cgattgccac cgcaatcggc aagtgtccga caccgaaaac
     1621 aatcgacgaa atcaggatgg cgcctacaaa gcaaactgat cccggctgac gtcgtcctct
     1681 ctggaaaact ctccacgccg cccataccag cagcgtgagc agaccccacc tcagaagtaa
     1741 ttcttccgtg atgcctccgt acaaaatgcg cgtaggcagc gggaccagac ggttgaacgc
     1801 ctcggctcgc gccacaaact ccgcgggcag aaaggatctg aagagggccc agctaaggac
     1861 gatgacgaca gccccgatca atccggcaat cacgccggga atgatttgcg gcttgagcgc
     1921 tgcgatgaag tttccgcggt gcgccaatgc ctcaaaggcc ggggcagaca gacttacctt
     1981 gggagccagt aagacgccga cgagcaccgc cagggccagc aacagcgtag gctgaatcag
     2041 gctcagaaat tttagcaacc tgggcgagtt cgcaagttca ttcccgtctg ttgtcggcag
     2101 aaccgccagg agcgccgaca gatcaaccag caggaaagag a
//
LOCUS       DASUEX010000249         3427 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1540202, whole genome shotgun sequence.
ACCESSION   DASUEX010000249 DASUEX010000000
VERSION     DASUEX010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3427
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1540202"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(218..1174)
                     /locus_tag="VFV61_04225"
     CDS             complement(218..1174)
                     /locus_tag="VFV61_04225"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX4899713.1"
                     /translation="MKKTFAVVSLLIFAFACAAPPTNDVATNRAANVAADKPAAMAMT
                     EADAIAKEKAIWETIRVKDYEAFGNMLASDQIEVLDVGINDKAASIAGVKDFEPSEVV
                     FSDWKYVPIDKDAFVVVYTANMKGKYKGKEFEPQSVRASSAWVARDGKWVAIFHQECP
                     VKPATPAPASATSAKPMASPSTAPMAAATAGSDPIANEKTVWDLIKGKNFDGFASLLA
                     ADFIEVEPEGVYDKAGSVKGVTTFDASKAVLSDFKAANIDADAAIVTYVATIPKLAPK
                     GDRHSTIWVHRDGKWVAILHHGGTPVAPMPAAPKASASPAMK"
     gene            complement(1344..2039)
                     /locus_tag="VFV61_04230"
     CDS             complement(1344..2039)
                     /locus_tag="VFV61_04230"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HEX4899714.1"
                     /translation="MRKTSLAAAMVAVVSLFAFVQIACQPAADTNRSESMAPPNTNSG
                     RETVDTAAIETELLRIENDWPRVVKEKDVAAVGRVEADDAIFVYPDGSVGNKQQDLSD
                     IGGGALTADTILMTDLKVKVLDSDAAFVTGQTEFKNAKYKTPDGKAMDISGKYRFIDT
                     FARRNGQWQLVAGISTKITATATPTASPAASPAMKATPAAKASPEVKASPAASPAVKA
                     PANTSPPMTKGMP"
     gene            2479..2967
                     /locus_tag="VFV61_04235"
     CDS             2479..2967
                     /locus_tag="VFV61_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899715.1"
                     /translation="MSYPLIKRTARFRNLLLLCTALCGALLLMPNALGQKRNEEPEKP
                     LFQEYRGIQLGMAADEVRKKLGNPTDKGDEQDFFMLNDAESAQVFYDKTKKVMAISAN
                     FLQGAQNVPTPKVLFGEELAAKPDGSVYKMTRYPKAGCWLSYSKTAGDSPLIVVTLQK
                     ID"
     gene            3032..>3427
                     /locus_tag="VFV61_04240"
     CDS             3032..>3427
                     /locus_tag="VFV61_04240"
                     /inference="COORDINATES: protein motif:HMM:NF019931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueG-associated DUF1730 domain-containing
                     protein"
                     /protein_id="HEX4899716.1"
                     /translation="MYRASETFTPTAADPPPRGFSDHIRQQAGELGFELVGIVPAAAL
                     SAEQERLREWLARGYHGEMAWMARDPEKRTDPRKVFPAARSIIVVALNYYTPEQHSSA
                     PGSGKVSRYAWGDDYHDVVGDKLRDLLSWI"
BASE COUNT          741 a          931 c          916 g          839 t
ORIGIN      
        1 aacccgggta atcgccggtt ctcgaaacag gatggatcgg ttagttgaat gcggtcgcta
       61 acgctccggg ctccgtattc ttcgctgttc tctgctgcac ttcgaaattg aatcggcgat
      121 tcaaacagaa atcccaagtc ccacgcccca agcgaacaaa acttgggacc tgagacttgg
      181 gacctcggac ttgagacttg ggacctgaga cttgagacta tttcatcgcc ggcgaagctg
      241 acgctttcgg cgccgcgggc attggcgcaa ccggagtgcc gccatgatga aggatggcca
      301 cccacttccc gtcccgatgc acccagatcg ttgagtgacg atcaccctta ggcgcgagct
      361 tggggatggt ggcgacataa gtcacaatcg cggcatccgc gtcgatgttg gcagccttga
      421 aatcacttaa tacagctttc gaagcatcaa acgtggttac acctttgact gatcctgctt
      481 tgtcgtaaac gccctcgggt tcaacctcaa taaagtccgc agcgagcagg cttgcaaacc
      541 cgtcaaagtt tttgccttta atgagatccc aaacggtctt ttcattggcg atcggatccg
      601 agcccgctgt agctgctgcc atcggcgcgg tggaaggcga cgccattggc ttggcagaag
      661 tcgcagaggc gggggcggga gtcgccggct ttaccggaca ttcctggtga aagattgcga
      721 cccacttgcc atcgcgagcc acccaggccg aacttgcgcg cacagactga ggctcaaact
      781 ctttgccttt atactttccc ttcatgtttg cagtgtagac aactacaaac gcgtctttgt
      841 cgatgggcac atacttccaa tcactaaata caacttcgct tggttcgaag tcttttacgc
      901 ctgcgattga tgcagctttg tcgttgatac caacgtcgag aacttcgatt tgatcgctgg
      961 ctaacatatt gccaaaggct tcgtagtctt tgactctaat tgtttcccag atcgcttttt
     1021 ctttggcgat cgcatccgct tcagtcatcg ccatggcagc cggcttgtcg gcggccacat
     1081 tcgctgcacg gttagtcgca acgtcgttag taggcggagc cgcgcaagcg aacgcaaaga
     1141 tcaataaaga cacgactgcg aaagtctttt tcatgatgta actcctggac aggtgttgtt
     1201 gatgtagcgc cgcggtttta tgccaattgc ccgaattagt caaggaagtg ctttgcccca
     1261 atggactaac gtcttaagaa gagccgcgcc gaagtgatcg acaagctacg gcgcggcgat
     1321 ttattttcct ttctggttcc ttcttacggc atccctttcg tcattggtgg actcgtgttg
     1381 gcgggtgcct tcaccgctgg cgaggccgct ggcgatgcct tcacctctgg agacgcttta
     1441 gccgctggtg tagctttcat tgccggagaa gccgcaggtg acgccgtggg ggtcgccgtc
     1501 gccgtaatct tcgtcgagat acctgcgact agttgccact gaccattgcg ccgggcaaag
     1561 gtatcgatga aacgatactt gccgctgata tccatcgcct tgccgtccgg cgtcttgtac
     1621 ttcgcgttct tgaactcagt ctgacccgtt acaaaggcag cgtcgctgtc gagtactttc
     1681 accttcaggt cagtcatcaa gatggtatct gcggtcaatg ctccaccgcc gatgtcgctt
     1741 agatcctgtt gcttgtttcc tacagagccg tcgggataga cgaagatggc gtcatcagct
     1801 tcaacgcgtc caactgcggc cacgtctttc tccttgacga cgcgcggcca atcgttctcg
     1861 atgcggagca gttccgtctc gatcgcagcg gtatcgactg tttctcttcc ggaattagta
     1921 ttcggaggag ccatggattc cgagcgattt gtatctgccg ccggctggca ggctatttga
     1981 acaaaagcga agagcgatac gaccgctacc attgcggccg cgaggcttgt ttttctcatc
     2041 tgagcagctc ctttgttaag tacgcaactt tgatttgcct actgggtcgt acgagaaacg
     2101 attgattggt gtgcgggcca agggctatat gccaaaacag tccgcgagtc aatggtcctc
     2161 attgccaatt ttcaattgcg gattgtcgat ttgctccgcg ggtttagcga ccctatctct
     2221 cgacaatcaa ctcaatgatt actccgccgc tggcgccaaa aatgagaatc ggcgcgtttc
     2281 acgtgaagtc aggccccgtg tcggtgcact aaatgtcctg gactgagcga gagcattgac
     2341 aggacgaaac tgatgggatt attgtccact caatcggacc cgatggacag gccgttcggg
     2401 cagcttcttt caacaaatcc gccggttctg gcctctgacc cttcataaat cacattctct
     2461 gtattaggag aaaacccgat gagttaccca ctgattaagc gtacagcacg attccggaac
     2521 ctgctgcttt tgtgcaccgc gttgtgcgga gcgctgcttc tgatgccgaa cgcgctggga
     2581 caaaaacgaa atgaagaacc agaaaagcct ctttttcaag agtatcgcgg gatccagtta
     2641 ggtatggctg ccgacgaagt tcgtaagaag ctaggcaatc cgactgacaa aggcgatgag
     2701 caggattttt ttatgctcaa cgatgccgaa agtgcgcaag ttttttatga caagactaag
     2761 aaggtgatgg ctatctcggc caactttctc cagggcgccc agaacgttcc cacgccaaag
     2821 gttctgtttg gtgaagagct ggcagcaaag cctgacggct cagtttataa gatgacgcgt
     2881 tatccaaaag ctggatgttg gttgtcgtac agcaagactg cgggcgactc gccgctgata
     2941 gtcgtcaccc tgcaaaagat cgattagctg cggttatttc atccagactc atcactggac
     3001 gcccgctccg tccacgctta tcctgactgg cgtgtatcga gcgagcgaaa ctttcactcc
     3061 aacggcggct gatccacctc ccagaggttt tagcgatcac attcgacaac aggccggcga
     3121 gctgggtttt gagctcgtgg gcattgttcc cgctgccgcg ctcagcgcgg agcaggaacg
     3181 attgcgcgag tggctggcgc gtggttacca cggcgaaatg gcgtggatgg cacgcgaccc
     3241 ggaaaagcgc acagatcctc gtaaggtttt cccagcggcg cgctccatca ttgtcgtcgc
     3301 tcttaactac tacacaccgg agcaacactc gtctgcgccc ggcagcggta aagtctcacg
     3361 atatgcctgg ggcgatgact atcacgacgt cgttggtgac aaactgcgcg acttgctttc
     3421 gtggatt
//
LOCUS       DASUEX010000250         2286 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_265596, whole genome shotgun sequence.
ACCESSION   DASUEX010000250 DASUEX010000000
VERSION     DASUEX010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265596"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(227..676)
                     /locus_tag="VFV61_04245"
     CDS             complement(227..676)
                     /locus_tag="VFV61_04245"
                     /inference="COORDINATES: protein motif:HMM:TIGR01994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEX4899717.1"
                     /translation="MSELSELYQQVILDHNKKPRNFRKLELANHSAEGYNPLCGDHLT
                     VYLNLEDDTVKEVTFEGSGCAISKAAASMMTQAVKGKSKQDAEELFTAFHRMVTGEDY
                     ETLAAERLGNLKIFEGVREFPVRVKCATLPWHTMHAALNNKELVSTE"
     gene            complement(772..2019)
                     /locus_tag="VFV61_04250"
     CDS             complement(772..2019)
                     /locus_tag="VFV61_04250"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020359689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEX4899718.1"
                     /translation="MDDANRVAADAWDVERIRADFPVLHQTVNGKPLVYLDNAASSQV
                     PQVVIERGSMYLEQEHSNIHRGVHYLSQKATTAYEGARETVKRFINAREAKECIFVRG
                     TTEGINLVMHGYGRKFIEAGDEIIISAMEHHANIVPWQMLCEEKGSRLRVIPMNDAGE
                     LLLDEYHGLLNERTKFVALSHVSNALGTINPVKEMIEHAHKYGVPVLIDGAQSAPHMP
                     VDVQDLDCDFFAFSGHKMFAPTGSGVVYGKAAILERMNPFQGGGDMIRNVTFEKTTYA
                     DLPNKFEAGTPAIASQIGLGAAIDYLNGIGREQAAIHEQELLRYATERLSAIEGLRII
                     GTARHKASVLSFVIDEIHPHDIGTILDQEGIAVRAGHHCAQPVMQRFKVPATARASFA
                     FYNTKAEVDVLAATIRKVIDIFS"
     gene            complement(2053..>2286)
                     /locus_tag="VFV61_04255"
     CDS             complement(2053..>2286)
                     /locus_tag="VFV61_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899719.1"
                     /translation="FSLVSLLGALTLTFLLRKRAPESLLKGVVAIHGFVFGICFAIMA
                     AFTFLPPIILTGLIFVCLLIGFLLIPKQTPLVS"
BASE COUNT          545 a          629 c          579 g          533 t
ORIGIN      
        1 gcttaaagtt accgactcgt ccggcaatgt gacgacggct attagcacag tgactgtgcc
       61 actaagtccg ggagctggtg cggcggttga tagtgggcca atgtatacgg tcagtggcag
      121 ctgtccgtag aatccaagtg ctggaaagaa gaagaggact ggacgattaa atcgcccagt
      181 cctttttcgt tatggagacg tgcaacgcct ggcgccgatt gcagctctat tccgtggaaa
      241 ctaattcctt attattgagc gcggcatgca tcgtatgcca gggcaaggtc gcgcatttta
      301 cgcgcactgg aaactctcga acaccttcaa agatcttaag attgcccaga cgctctgcag
      361 ccagagtttc gtaatcctcg cccgtcacca tgcggtgaaa cgcagtgaaa agctcttctg
      421 catcttgctt gctcttcccc ttcacagcct gggtcatcat gctcgcggcc gcctttgaga
      481 tggcacaacc ggagccctca aaggtgacct ctttgacagt atcgtcttcg agattcaaat
      541 agacggtgag gtgatcaccg cagagagggt tgtatccttc ggcagaatga ttggccaact
      601 caagcttgcg aaagtttcgc ggctttttgt tgtgatccag aatgacctgc tgataaagct
      661 cgcttagttc tgacatatat gaattgccga ttgacgattg gcgattgata agacagcgat
      721 tcttacactt aactattcac gattcacgat tcacaattta cgattcacga atcacgaaaa
      781 tatatcgatg accttgcgga tcgtggcggc gagcacatcg acttccgcct tcgtgttgta
      841 aaaagcgaat gacgcgcggg cggtcgctgg caccttaaaa cgctgcatta ctggctgcgc
      901 acaatggtgg ccagcgcgca cggcaatgcc ttcctgatcc agaatggtac cgatgtcgtg
      961 cggatgaatc tcatcgatta cgaaagaaag cacactggcc ttgtggcgcg ccgtgccaat
     1021 aatccttaga ccctcgattg ctgataacct ttccgtcgca taccgcaaca attcctgctc
     1081 atgaatcgcc gcctgctcgc gaccgatgcc gttcaagtaa tcaatcgcgg ctcccaagcc
     1141 gatctgtgaa gcaatcgccg gcgttcctgc ttcaaacttg ttcggaagat cagcgtaggt
     1201 tgtcttctca aaagtcacat ttcggatcat gtcaccaccg ccctggaaag gattcatccg
     1261 ctccaggatg gcagccttgc cataaacgac accgctgccg gtcggcgcaa acatcttatg
     1321 gccggaaaac gcgaaaaagt cgcaatcgag atcttgaaca tcgacgggca tatgcggcgc
     1381 actctgcgct ccatcgatca gcaccggcac gccatactta tgggcatgct caatcatctc
     1441 cttgactggg tttatcgttc ccagcgcatt ggaaacatga ctgagcgcga caaacttggt
     1501 tcgctcgttg aggaggccgt ggtactcgtc caaaagcagc tcgccggcgt cgttcatcgg
     1561 gatcacgcgc aggcgagatc ccttctcttc gcagagcatc tgccagggca caatgttggc
     1621 atgatgctcc attgcggaaa tgatgatctc atcacccgct tcaatgaact tccggccgta
     1681 gccatgcatc acgaggttga taccttcggt ggtgccccgc acaaaaatgc attccttggc
     1741 ttcacgcgca ttgatgaacc gttttacggt ttcgcgcgca ccctcgtacg cggtggtcgc
     1801 tttctggctg aggtaatgga cgccccgatg aatgttcgag tgttcctgtt ccagatacat
     1861 gctgccgcgc tcgattacaa cttgcggcac ttgcgaggac gcggcgttat ccagatagac
     1921 cagtggtttg ccgttcacgg tctgatgcag gaccggaaag tccgcccgga tacgctccac
     1981 gtcccaggcg tctgcagcaa ctcgattcgc gtcatccatg cttgttactc ttgtcgccga
     2041 catcctcaat ccttacgaaa ctagaggcgt ctgtttcggg atcaacagaa acccaattag
     2101 cagacaaaca aaaatcaagc ccgtcagtat gatgggaggt aagaaagtga aggcggccat
     2161 gatcgcgaaa cagattccaa acacaaatcc atgaatggcc acgacccctt tcagcagcga
     2221 ctctggtgct cgtttgcgca gcaagaaggt cagggtcaag gctcccaaca agctgaccaa
     2281 agaaaa
//
LOCUS       DASUEX010000251         3033 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_623135, whole genome shotgun sequence.
ACCESSION   DASUEX010000251 DASUEX010000000
VERSION     DASUEX010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3033
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_623135"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2956)
                     /locus_tag="VFV61_04260"
     CDS             complement(<1..2956)
                     /locus_tag="VFV61_04260"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX4899720.1"
                     /translation="MPFRLSPRPLPSRKAVRLVVLCALLIAAVVEGNAQDTVTGAFEG
                     IVSDSQTGLPLKGAAVEIINQQTGVTFNIRTDYRGSFFQGLLLPGIYTVRVSMPGYTT
                     KVVPQRLRITYTGEVVPVPVALDPEPAVAPTATPAPAAIATADDVDIRATIGTTDGRR
                     SGSFTETEVVTLPLGAATFTRTFDELALLLPGVAPPPQTSGSVAGPGVGSGVGSAGQF
                     AVNGLRSRGNNFTVDGSDNNDEDIGVRRQGFLELVPQPLESIQEYQVITALAPAQFGR
                     NLGAQVNAVSKSGGNAHHGTLYGLFNSSQLNARNFFDTNNGNAISPARTATNQPVLLD
                     GAPILIRNDSGGKDSFSLAQGGFVVGGPASRDRTFYFISFEGQILNATKEESFAVPIV
                     EQRGAFNTGASALFRNPLGPRDANGNLTEQVFAYPTTVQGDAVLSLYPFPNNPTGVYG
                     ANTFTQVLPASGRGAVFSGKLDHNFRTRGRHHSLTGRYNFTDDGRDIPAVNGALFSTI
                     RARVRTQNFSFFANSELSAPASNRPLFNQLRLSYGRTRLNFEDRRPADFLRPSVSLPG
                     EPFLLNAPLRLNSTLPRVINNIQFEANQGPVRYTTDPNGPTVEAALGPIGQVNIAGFS
                     PVGVDVFNFPQRRVNNTYQAADTVTYNASRHRLAFGVDTRRTELNSELARNSRPLITF
                     NGAPELDVTPAGTLAYSNRFIRPETFAAASAASGFSQTLATGGESAINLRFYQLDFFA
                     QDEWRVREGLSFSYGLRYEYNTPPREVNRRIEDTFDDPSLDLIPGLRQFLAGRTQMFD
                     PDRNNFAPRIGVAYSPKLFGRDKTTVFRGGYGIFYDQILGAVVSQSRNVYPTYLTLDL
                     AGGFANLRFVPGSNQFGDSCNAPNRCPYELINPQIATLFGTRLVVPGTLNTRNPNVSF
                     AQIAGIVNLVGGGPVPAQSGFGVTLPARHLEMPMGQHYSVTFEQQLGRQLVISAAYVG
                     TKGSSLLRFNTPN"
BASE COUNT          709 a          820 c          867 g          637 t
ORIGIN      
        1 agtttggtgt gttgaagcgt agcagcgaac tacctttcgt tcccacgtac gccgcggaga
       61 tgaccaactg tcggcccaat tgctgctcaa aggtcacgct gtaatgctga cccatgggca
      121 tctcgaggtg acgtgccggc aaagtcacgc caaagccact ctgcgccggc accggtccac
      181 ctccaaccag attcacaatg ccggctatct gcgcgaagct gacgttcggg tttctggtgt
      241 tgagtgtccc ggggacgacc agcctggtgc caaacaacgt cgcaatctga ggattgatca
      301 attcgtaggg acaccgattc ggcgcgttgc acgaatcgcc aaactgatta ctgccgggca
      361 cgaagcggag gttcgcaaag ccaccggcga gatccaaagt caggtaagtg ggatagacgt
      421 ttcgcgattg actgacgacc gcacccagaa tctgatcgta gaagattcca taaccacctc
      481 ggaacacggt ggtcttgtcg cgtccaaata attttggaga ataggctact ccgattcgcg
      541 gtgcgaagtt gttgcgatcg ggatcaaaca tctgggtacg cccagcgaga aactggcgca
      601 acccgggaat caaatcaagc gaaggatcat caaaagtatc ttcaatgcgc cggttcactt
      661 cgcgcggcgg tgtgttgtac tcgtaacgca acccgtaaga gaacgacagg ccttcccgca
      721 cccgccattc atcctgagca aaaaagtcca gctggtaaaa gcgcagattg atcgccgact
      781 caccacccgt ggcaagggtc tgcgagaaac cacttgcggc cgacgccgct gcgaatgttt
      841 ccgggcgaat gaagcgattc gaataggcga gagttcccgc aggcgttaca tcaagttccg
      901 gagcgccgtt aaatgtgatt aacggtcgcg aattacgcgc tagctcgcta ttcagctcag
      961 tgcgccgcgt gtcgacacca aaagccaaac ggtgtcggct cgcgttgtag gtgaccgtgt
     1021 ctgcggcttg ataggtgttg ttaacgcggc gttgcggaaa attgaaaaca tcaacaccaa
     1081 ctgggctgaa gcccgcgatg ttgacctggc cgattggtcc cagggctgct tcgactgttg
     1141 gtccgttggg atcagtcgta taacgcaccg ggccctggtt cgcttcaaac tgaatgttgt
     1201 tgattacgcg aggcagcgtg gaattcagtc gcagtggagc attcagcaga aaaggctcgc
     1261 caggcaaact aacgctggga cgcagaaagt ccgccggtct tcgatcctca aagttcagac
     1321 gcgtgcgtcc gtaagacagt cgtagttgat tgaacaacgg tctgttcgaa gcgggggcgc
     1381 tgagctcgct attggcaaag aatgaaaagt tctgcgtccg cacccgcgcg cggatcgtcg
     1441 aaaacagggc gccgttaact gccggaatgt cgcggccatc gtcagtgaag ttataacggc
     1501 cagttaggga atgatgtcgg ccacgcgtgc gaaagttatg atccagcttt ccggaaaaaa
     1561 cagcgccccg gccactcgca ggcaaaacct gcgtgaaggt attggcccca taaacgccgg
     1621 tcggattgtt tggaaatgga taaagactca aaacggcgtc gccctgtacc gtggttgggt
     1681 atgcaaagac ttgctcagtt aagttaccgt ttgcgtctcg aggcccgagt gggttgcgga
     1741 acagtgcgga cgcgccagta ttgaacgcgc cgcgctgttc gactatgggc accgcaaaac
     1801 tctcctcctt cgtagcattc agaatctgcc cctcaaatga aatgaagtag aacgtacgat
     1861 cgcgagacgc cggaccaccc acaacgaaac cgccctgtgc cagcgaaaat gaatccttac
     1921 cgccactgtc gttcctgatc aggatcggcg ccccatccag caagacaggc tgattagtcg
     1981 cagtacgcgc gggtgagatg gcgtttccat tattcgtgtc aaaaaagttt cgggcattca
     2041 actggcttga attgaagaga ccatagagag ttccgtgatg agcgttaccg ccggattttg
     2101 acaccgcatt tacctgcgca cccaagttgc ggccaaattg cgccggcgcc agagccgtga
     2161 tgacttgata ttcctggatc gactccagcg gctgcggaac taattccaaa aaaccttgcc
     2221 tccgaacgcc gatgtcttcg tcgttgttat cggacccgtc taccgtgaaa ttgtttccgc
     2281 gagagcgaag gccattgacg gcgaattgtc cggctgagcc aacgccggaa cctactccag
     2341 gtccagccac actgcccgaa gtctgtggcg gtggggcaac accaggcagt aacaacgcca
     2401 gctcatcaaa ggtacgagtg aacgttgctg ctccgagggg cagagtgacc acttcggttt
     2461 cggtaaatga accacttcgc cttccatctg tggtgccaat ggttgcccga atgtccacat
     2521 catccgcggt cgcgattgct gcgggtgctg gtgtggctgt gggcgccact gcgggctccg
     2581 ggtcaagtgc caccggcact gggacaactt ctccggtgta ggtgatgcgc aagcgctgcg
     2641 gtaccacctt ggttgtgtaa ccgggcatgg agacacgaac ggtatagatc ccgggtaaca
     2701 acaggccctg aaagaagctg ccgcggtaat cggtgcgaat gttgaacgtc acgccggttt
     2761 gctgattaat gatttccacg gccgcgcctt tcaggggcag acctgtctga ctgtcactaa
     2821 cgattccttc aaaagcgccg gtgacagtgt cctgtgcatt gccttccaca acggcagcaa
     2881 taaggagggc gcagagcact accagtcgaa ctgcttttcg ggaaggtaat ggtctgggag
     2941 acaacctgaa aggcattaat gttttccctt ctaatcgaga tggcaaaact gtgcgaacaa
     3001 tttcagggcg tggttccgat tgaagatgcg ggc
//
LOCUS       DASUEX010000252         2308 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_852659, whole genome shotgun sequence.
ACCESSION   DASUEX010000252 DASUEX010000000
VERSION     DASUEX010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_852659"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1307)
                     /locus_tag="VFV61_04265"
     CDS             complement(<1..1307)
                     /locus_tag="VFV61_04265"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEX4899721.1"
                     /translation="MDDLRKFVRYFRPYKASLIIGILCILAGVLFNLSIPLIVGQAID
                     ENWKTLSWSSLTISALKVLGASVASGTFLYLQRRILIGISRNIEYDLRKDFYAHLVDQ
                     PLSFFQEHRVGDLMARASNDLSAVRQLAGPMIMYTLQTVFVVLLVLPLMLMLNWWLTF
                     LLFVTMPLVSLTVKFFGQQVHVRFEKIQDFFAQITARAQENFTGVRVVRAYAQEGAEI
                     AAFNSLNREYAEKNLSLVRIDALMRPLMQFLIGIGFVLIIWAGVPLAIRGEISVGDFT
                     VFNMYLMRLIWPLIALGYVVNLFQRGTASLKRMNAILAVKPAIADAPHVSPQQPIAGE
                     IEFRELTFKYRPTDEPVLRDINLKIPVGQSVAFVGRTGSGKSTLVNLIPRLLEAPEDT
                     VFVDGVSVRKYPLAQLRESIGYVPQETFLFSDTLAENVAFGVN"
     gene            complement(1395..>2308)
                     /locus_tag="VFV61_04270"
     CDS             complement(1395..>2308)
                     /locus_tag="VFV61_04270"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HEX4899722.1"
                     /translation="IGGELTKCAFNVSENDGRSERLATHLDAIGYRLTITPKIYVDDD
                     NHRETSSEFAQAAVPLIFNGNSIGFLLVRSDDPKRVWADNELLLLDTVADQLTVSVNQ
                     ARLFAEMEQQALTDALTGCYNRRSFDLQLERDLQLAIRMRQPLSLVMIDLDYFKRIND
                     MAGHNTGDIALRMMADVMRTELRAVDTAVRLGGDEFAVILPQANVNGALIVAERLRAR
                     IEKDDVPGYGHITASLGVASFPHHASSRENLVVAADRALYNSKHCGRNCVSTPPEEIL
                     TPPGAGEVDFSGEKLIDSAAETLTLPG"
BASE COUNT          576 a          616 c          625 g          491 t
ORIGIN      
        1 tggttcacac cgaaagcgac attttcagcg agagtatcgc tgaagaggaa ggtctcctgc
       61 ggtacatagc cgatgctttc gcgcaactgc gcgaggggat acttgcgcac cgagacacca
      121 tcaacgaaga cagtatcttc cggcgcttcc aggaggcggg gaatcaggtt caccaaagta
      181 gatttcccgc tgcctgtccg ccccacaaaa gccacagact gtcccacggg aatcttcaag
      241 ttgatgtcgc gaagtactgg ctcatctgtg ggccggtact tgaaagtgag ttcgcgaaac
      301 tcaatctctc cggcaattgg ctgctgcggg ctgacgtgtg gagcatccgc aattgcaggc
      361 ttgaccgcta gaatcgcgtt catgcgtttg agacttgcgg tcccgcgctg gaagagattg
      421 actacatacc cgagggcgat taacggccaa atcagtcgca tcagatacat gttgaagact
      481 gtgaagtcgc caacactaat ctcgccgcgg attgccagcg gcactcccgc ccaaatgatc
      541 agcacaaagc ctatgccgat cagaaactgc atcagcgggc gcatgagagc gtcgatcctc
      601 accaggctca ggttcttctc tgcatactcg cggttcaatg agttgaaagc cgcaatctcg
      661 gcgccttcct gggcataagc gcgcaccaca cgaactcccg tgaagttttc ctgggcccgc
      721 gccgttatct gggcaaagaa gtcctggatc ttttcaaaac gtacgtggac ctgctgaccg
      781 aaaaacttca ccgtgagaga gacgagtggc atcgttacaa agagcaggaa cgtcagccac
      841 cagttcagca tcaacatcag cggcaatacc agtaagacca cgaacacggt ttgcagcgtg
      901 tacatgatca tcggccctgc gagctgacgc acggcagaga gatcatttga ggctcgcgcc
      961 atcagatcgc ctacgcggtg ctcctgaaaa aacgaaaggg gttggtctac caggtgagcg
     1021 tagaagtctt tccgcaagtc gtattcgatg ttgcgcgaga tgccgatcag gatgcgccgt
     1081 tgcagataaa ggaaagtgcc gcttgccaca ctggcgccga gaaccttcag cgcagagatc
     1141 gtcagacttg accaggacag agttttccag ttctcgtcaa tcgcctgacc aacaatcaaa
     1201 ggaatgctga gattgaagag cacaccggca aggatgcaga gaataccgat gattaacgat
     1261 gccttgtaag gtcgaaaata ccggacaaat ttacgcagat cgtccatcgc aaaacgcgct
     1321 acagatgaaa gccctcagaa ttatgaaagc agtatgatgc gcgatttttc gttgggacgc
     1381 cagaagcgac tcgattagcc gggcagtgtc agtgtttcgg cagctgagtc gatcagcttc
     1441 tcgccagaga agtcgacttc gccggcacca ggcggggtca agatttcttc tggtggtgta
     1501 ctgacgcaat ttcgtccgca gtgttttgag ttgtaaagag cgcggtcagc ggcaacaaca
     1561 agattctcac gtgaggatgc gtgatgtgga aaactggcca ctccaagtga agcagttatg
     1621 tgtccataac ctggcacatc atccttttca atacgggcgc gcaaacgctc ggcgacaatt
     1681 aaagcgccat taacgtttgc ctgcggcaag atgacggcaa attcgtcgcc acctaaccgg
     1741 actgcggtgt cgacggcgcg cagttcggtg cgcatcacat cagccatcat gcgcaacgca
     1801 atatctccgg tgttatgtcc ggccatatca ttgattcgct tgaagtaatc aaggtctatc
     1861 atgaccagcg acagaggttg ccgcatgcgt atggccaatt gaagatcgcg ctccagttga
     1921 aggtcaaacg agcgtcggtt gtaacaaccg gtcaatgcgt ccgtgagcgc ttgctgctcc
     1981 atctccgcaa agagtcgcgc ttggttcacg gagacggtga gctggtcggc gacggtgtcc
     2041 aacaataaca attcgttgtc cgcccagacg cgtttcggat cgtcggaacg cactaataga
     2101 aaaccaattg agttgccgtt gaaaatcagg ggcaccgccg cctgggcaaa ctcggaagat
     2161 gtttcgcgat ggttgtcatc gtcaacatag atcttcggcg ttatcgtcaa gcgatagcca
     2221 atcgcgtcga ggtgcgtagc caacctttcg gatcggccat cgttttcgga cacgttaaaa
     2281 gcacacttcg tcagttcacc gccgatca
//
LOCUS       DASUEX010000253         4755 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_852863, whole genome shotgun sequence.
ACCESSION   DASUEX010000253 DASUEX010000000
VERSION     DASUEX010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4755
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_852863"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(201..1208)
                     /locus_tag="VFV61_04275"
     CDS             complement(201..1208)
                     /locus_tag="VFV61_04275"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX4899723.1"
                     /translation="MYSPTKDALKIALASLFVLIFAADQRNRVAFAQEQGSDKPLAAP
                     SAQQPGREQDGPVIVNTDLITLTVTVTDTYGRYVSGLGKNAFTILDNKEAQDISFFSD
                     DDAPVSVGVIFDVSGSMSGDKIKRARDALANFIQTSHNADEYFLIAFNSRAQLLLDKT
                     RDGDAVLDKLTFVQTKNQTALYDACYLGVEKVQRGAHPKRALLLISDGQDNKSRYTFN
                     EVRRLLKESDVVLYAVGILSGSDAGSSLGMEGQGILDELANVSGGKAFFPRSAAEMDD
                     IFEQIALELRHQYSIGYKPTDFNNDGRWHRIKVKVTPPRGLPKLFVRSKEGYYAIPGS
                     R"
     gene            complement(1537..2559)
                     /locus_tag="VFV61_04280"
     CDS             complement(1537..2559)
                     /locus_tag="VFV61_04280"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEX4899724.1"
                     /translation="MTANASEGPPPQATMLQLISGFWISRAIYAAAKLGIPDLLHNTE
                     KSAEQLAEATGSHARSLYRILRALVSVGVFAEKDGRFSQSPLSETLRTDAPGSVRWFT
                     ISELGEEHYPAWGDLMHSVKTGGIAFDHVFGVDIWKFFSGHPEHAKVFNDSMSSLTAA
                     ANKAIIASYDFSQIRKIVDVGGGHGALITSILNANPALKGILFDAPSVVAGAAERIQA
                     AGLGDRCETIGGDFFQSVPGGADAYILKWIIHDWDDERAITILQHCRSGITSDGRLLL
                     VDVVIPPGDEPHFGKFMDLNMLVITGGLERTEAEFKKLLASGGFSLVRVVPTESPFSI
                     VEARPA"
     gene            2888..4129
                     /locus_tag="VFV61_04285"
     CDS             2888..4129
                     /locus_tag="VFV61_04285"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX4899725.1"
                     /translation="MAEFICRLGTPAGDVVTRTVEAQGVAEARARLEREGFKVFNVTP
                     PKTAGVTALTRLGGRGSAPRVKANDFLLFNQQLAALSRAGIPILQAISMLRKRAASAR
                     LRAVLGDVEEQIRGGAALSQAFASQGGIFPRIYTASILAGERSGALDDVLNRYVSYMR
                     RNVALRRKIRGALAYPLFLLFASGCMVTFLTVYVVPRMSELFAGFGNELPTVTQIVVG
                     ISRWLSGNVIWFGPLLIVGAIALFLWSRTTQGRLKLDALVLKLPLAGSLVVQLSVAQA
                     TRSLATLLAGGITLVESWEIAAESITNRELRRRSSAILPMILEGRSFTESLESAGWVP
                     ALAIDMIGIGERSGSLREMLDEVAVFYDAESEVRLEQLTTTLEPAILVVMGGVVVTIL
                     LAIYLPIIQSISSGAGVTGHR"
     gene            4181..>4755
                     /locus_tag="VFV61_04290"
     CDS             4181..>4755
                     /locus_tag="VFV61_04290"
                     /inference="COORDINATES: protein motif:HMM:NF017009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899726.1"
                     /translation="MEQITKNQTTPLASLPTAAQDQGDHEPPEVRAAKELAARYRLPF
                     VELLPRDEESPVDYEQFNQIPVDLMVRYQFLPLKRDGKGLHIAMADPTDLDRLDELAG
                     AVKARIVPYVATAGAIEVILRRGDATQRVLQEAASGFRISLVRETEHGEEVLDLDRLA
                     TDSEMSPIIKLVDTIIYNAMESRASDIHIETR"
BASE COUNT         1057 a         1283 c         1333 g         1082 t
ORIGIN      
        1 ccaacggcac gcgacacggc aaggtcggcg atatgactat gaaaacaaca cacgccagcc
       61 tatccaaaag ctggcaaaac aagagatgca aaaggagagt taatcagttg ccgaggtaag
      121 caggccaggc gaacttctgt agcgcaacct ttagtttgcg aatatctaag ccagcgccgc
      181 tttaccgtac gaccgcaaga tcagcgagaa ccgggaatcg cgtagtaacc ttccttcgag
      241 cgcacgaata atttcggcaa acctcttggc ggcgtgacct tgaccttgat gcggtgccaa
      301 cggccatcat tattgaagtc tgttggctta tatccaatcg aatactggtg acgaagttcg
      361 agcgcaattt gctcgaagat gtcgtccatt tcggcggcgc tgcgggggaa gaaggccttg
      421 ccaccggaca catttgcgag ttcatccaga atgccctgac cttccatgcc cagggagctg
      481 ccggcgtccg agccactgag gatgcccacg gcatacaaca cgacgtcgga ttctttcagt
      541 aatcgtctga cctcgttgaa tgtgtagcgt gatttgttgt cctggccgtc actaatcaga
      601 agcagtgcac gttttggatg ggcgccccgt tggactttct caaccccaag gtaacaggca
      661 tcgtaaaggg ccgtctggtt tttcgtctgg acgaacgtaa gtttatcgag gactgcgtcg
      721 ccgtcacgcg tcttgtcgag cagcaattgt gcgcgggaat tgaaagcaat caggaaatac
      781 tcgtcggcgt tatgactggt ttgaatgaag ttggccaggg catcacgcgc ccgtttgatc
      841 ttgtcgccac tcatagaccc cgaaacatca aagatcacgc ccactgaaac gggggcatcg
      901 tcgtcactaa agaagctgat gtcttgtgct tccttgttat cgagaatggt gaacgcgttt
      961 ttgcccaggc ccgaaacata gcggccatag gtgtcggtga cggtaactgt cagcgtaatc
     1021 aggtccgtgt ttacgattac cggaccatcc tgctcccggc ctggttgttg cgcgctgggg
     1081 gctgcgagag gcttatccga cccctgttcc tgagcaaaag caactctatt tcgttgatct
     1141 gccgcaaaaa tgagcacaaa aagagaggcc agcgcgatct tgagagcgtc ttttgtcggc
     1201 gaatacattg aaccctcgct ccacttaata ttcagattgt aggaacgggc aaagaggaac
     1261 tgccaacaca gatgctcaag ctgcaacgag tgatgcccga actggatgaa aatcttttga
     1321 gctttacctt ggggtggctt tgcgcaggag acgaacgctc gctcagttaa ctttcaaatc
     1381 ggttctgtat cggactttct gcattctaag acagcacctc tgggcacgca agaaactgcg
     1441 gtcctgaata ggctggtgca ggcgcaggct aagaaaaata gccctcattc cactcgattg
     1501 ccggccggcg caaaaccatt ttgtcggatt ggcttactaa gcgggccgag cttcaacgat
     1561 gctaaatggc gattccgtcg ggacaacccg aacgagggag aacccgccac tagctaagag
     1621 cttcttaaac tctgcttcgg ttcgttcaag tcccccggta atcaccagca tgttcaggtc
     1681 cataaacttg ccgaagtgag gttcatcgcc cggtgggatt accacatcga ccagcaacag
     1741 acgcccgtca ctggtaatgc cactgcggca atgttggagg atggtgatgg cccgctcgtc
     1801 gtcccagtcg tgaatgatcc atttcagaat gtaggcgtcg gctcccccgg gcaccgattg
     1861 aaagaaatcg ccgccaatcg tttcacaacg atcaccgagg ccggcagcct gaatgcgctc
     1921 ggctgcgcct gcgacaaccg acggtgcgtc aaagagaatt ccctttagcg cgggattggc
     1981 gttcagaatg gatgtgatca gcgcgccatg accgccgccc acatcgacga tcttcctgat
     2041 ctgcgaaaaa tcataactcg caatgatggc cttattggcg gccgccgtca gactactcat
     2101 cgaatcgttg aagaccttgg cgtgctcggg gtgcccgctg aaaaacttcc agatatcaac
     2161 gccaaaaacg tggtcaaagg cgatgccccc tgtctttacg ctatgcatca ggtcgcccca
     2221 ggcggggtaa tgctcctcgc ccaactctga aatagtaaac cagcgcaccg aacccggagc
     2281 gtcggttctg agggtctcgg acagtggtga ctgtgaaaac cttccgtcct tctcggcaaa
     2341 gacgccgaca ctaactagcg cacgcaagat tcgataaagt gatcgggcgt gactgccggt
     2401 tgcctctgcc agttgctcag cgctcttttc tgtgttgtgc aatagatcgg gaatgcccag
     2461 cttagcggca gcatagatag cgcgcgaaat ccagaagcca gagatcaatt ggagcatcgt
     2521 cgcttggggc ggcggccctt cggaagcatt tgctgtcatg ctgtttatcc tcactcaaaa
     2581 tggggtttga tgggagtaac ttagcacaac atttcatgtt ggaactacgc aacttcttcc
     2641 actcgcatgc gtgtacgagt cagtaccatc tgcggtagcg gatggggaca agccagggcg
     2701 attggagcag actcacattg gccaaccagg cgaagcgctt cataaccaca tatgaaccga
     2761 gcaggtagcg ggtacattag gcgcaccgtt tcggttccat ccgcaaccgc ggatggcttt
     2821 gacgtggcaa ggcattccat gcgtgtgata cctttcgttc tctgctagag gtcactcaga
     2881 aaaatcaatg gctgaattca tttgcagact aggaaccccc gcgggtgatg ttgtgacgcg
     2941 aacggttgag gcgcagggcg tcgccgaagc tcgggctcgc cttgaaagag aaggctttaa
     3001 agtcttcaac gtcactccgc cgaaaacagc gggtgtgacg gccttaacca ggttgggcgg
     3061 acgcggctca gcaccgagag tcaaagcaaa cgattttctc ttgttcaatc agcagttggc
     3121 cgcgctctcg cgtgctggaa taccgattct ccaggctatc tcaatgctgc ggaagcgtgc
     3181 tgcgtcagcg cgtttgcgag ctgtcctggg cgatgtggaa gagcagattc gtggcggggc
     3241 tgccctgtcg caggcctttg cgtcacaagg cggcatcttt ccacggatct acaccgcctc
     3301 tattctggcg ggcgaacgct caggcgcgct cgatgacgtt ctcaatcgtt atgtgagtta
     3361 catgcgtcgc aacgtcgcgc ttagaagaaa gattcgcggc gctctggcct atccgttgtt
     3421 cctgctcttc gcttcaggct gcatggttac cttcctaacg gtgtatgtcg tcccgcgcat
     3481 gtctgaattg tttgccggtt ttggtaatga gttaccaaca gttacgcaga tcgtggtcgg
     3541 tatttcgcgc tggttatctg gaaacgtaat ctggtttggc ccgctcttaa tcgttggagc
     3601 gattgcgctc tttctttggt cgcgcaccac gcaggggcga ttgaagttgg atgccctcgt
     3661 tctaaaactg ccgctggccg ggagccttgt cgtgcagttg tctgtggctc aggccactcg
     3721 ttcgctggcc acgctgttgg ctggaggcat cacgcttgtt gaatcatggg agatcgccgc
     3781 cgaatcgatc accaatcgag aactacggcg ccggagttct gccattctcc ccatgattct
     3841 cgagggccgt tcgtttactg aaagcctgga gtcggcgggt tgggtgccgg cgcttgcgat
     3901 tgacatgatt ggaattggcg agcgttcagg tagtttgcgc gagatgctcg atgaggttgc
     3961 cgttttttat gacgctgaat cagaggttcg cctcgagcag ttgacgacca cgcttgagcc
     4021 tgcgattctt gttgtcatgg gcggggtagt ggtgaccatt ctactggcca tctacttgcc
     4081 aattatccag tccatctcga gcggtgcggg tgtcaccggg caccgttagt atttgccagg
     4141 tcttcgcgtc gcgatgaact cggcgcacaa tcgacttcaa atggagcaga tcacaaaaaa
     4201 ccagaccaca ccgctcgcct cattgcctac cgcggcacaa gatcagggcg accatgaacc
     4261 gccggaggtg cgggcagcca aggagctggc agcacgttat cgcctgcctt ttgtcgaact
     4321 actgccacgc gacgaagaat ccccggttga ctacgagcag ttcaatcaga taccggtcga
     4381 cttgatggtg cgctatcaat tcctaccgct caaacgggac ggaaaggggt tgcacattgc
     4441 catggccgat ccaacggatc tggatcgcct ggacgaactc gccggggcag tcaaagcccg
     4501 catagttccc tatgtggcaa cggcgggcgc gatcgaagtt atcttgcgtc gcggcgatgc
     4561 aacccaacgc gtgctgcaag aggctgcttc aggatttcgc atctcgctgg tgagggagac
     4621 cgagcacggc gaagaagtac tcgatcttga tcggctggcg accgattctg agatgtcgcc
     4681 gataatcaag ctggtggaca cgatcatcta caacgcgatg gagtcgcgcg cgtccgacat
     4741 tcacatcgaa acgcg
//
LOCUS       DASUEX010000254         2262 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_852970, whole genome shotgun sequence.
ACCESSION   DASUEX010000254 DASUEX010000000
VERSION     DASUEX010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_852970"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            117..458
                     /locus_tag="VFV61_04295"
     CDS             117..458
                     /locus_tag="VFV61_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899727.1"
                     /translation="MRLRFLFLALVSVGGGYSVVHLRSVAHTAAPACNEATAVAPAPE
                     RIDFTTQVRPILARCQPCHFSGGTMYERLPFDRPETVTQLGEKLFSRIQAENERKIIR
                     EFLAQNQSNNK"
     gene            611..>2262
                     /locus_tag="VFV61_04300"
     CDS             611..>2262
                     /locus_tag="VFV61_04300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEX4899728.1"
                     /translation="MKHNVNFLRSLLDVSLDGILAFDRECRYTVWNREMECMSGVKSE
                     DVVGKPAFELFPFLVETGEDKYFHETLAGHRSVSRNRPFSVPQTGYEGFFEGHYYPLY
                     DDEGAVIGGLCVVRDITDQRHAYELAHEAHQRLTFHVQNSPLAVVEWDSDFCVSRWSE
                     SAERLFGWKAEEVLGKHVTDWHFVFADDVNDVVQLTSKQREGSETRGVLRNRNYTKDG
                     SIVHCEWYNSVLNDDEGRLVSVLSLVLDVTARELAAQERAKALLREREAREEAEKADR
                     LKDEFLATLSHELRTPLTSVLGWATLIRAGDIDAANYAIALETIERNARLQARLIDDL
                     LDVSRIITGNLHLELRPIDLAPVVEAARDAVRPTAEAKGIELRTSLAVGECSIKGDSN
                     RLRQVVWNLLLNAIKFTPRGGSVATRLHCRNSSVVLTVEDTGEGIEPDFLPHVFDRFR
                     QAEGSISRRQGGLGLGLAVVRHLVELHGGSVSAASEGKGKGATFTVELPLVGQEQAFV
                     ASNAAATLTRESNSTTTTAAGKDWRVRKGKNKPQTGKEKTAAS"
BASE COUNT          512 a          617 c          669 g          464 t
ORIGIN      
        1 aaggaatggc agcagcggat gcgcacgaaa cttagtttgc cgaccccacg tggcaacagt
       61 cgaccttgag gaacggggaa gttatgcgcg gcttgctgcg cggctaagaa agatttatgc
      121 gactgcgctt tttattcctg gcgttggtta gcgtgggtgg tggctacagc gtggttcact
      181 tgcgcagcgt cgctcacacc gcggcaccag catgcaacga agcaaccgca gtggctccgg
      241 cgcctgagag aattgatttc accactcaag tcaggccaat tctcgcacgc tgtcaacctt
      301 gccacttcag cggcggcacg atgtatgaac gcctgccctt tgatcggccg gagacagtga
      361 cccagctggg agagaagctc ttcagccgca ttcaggcaga gaacgaacga aaaatcattc
      421 gcgaattcct ggcacagaac cagagcaaca ataagtaacc gcgtttcgga tgccgaatgc
      481 caattgccga ttgaaaacgg gcaatgggga tgactcatcg atcgcgaaac gggtatgatg
      541 ccgttctgga accgtcactc ccgacctact ggcgggtctg gattcgaatc tgatcagtga
      601 ctaattcctc ttgaagcaca acgtaaattt tcttcgctca cttctggatg tcagtctgga
      661 cggcatcctc gcgtttgatc gcgagtgccg ctacacggtt tggaatcgcg aaatggaatg
      721 catgtccggc gtgaagagcg aagacgtcgt tggcaagccc gcttttgaac tctttccctt
      781 tctcgtcgaa acgggcgaag acaaatattt tcatgagacc ctcgccggtc accgcagcgt
      841 ttccaggaac cgccccttct ccgtgccgca gaccggctat gaaggctttt tcgaagggca
      901 ctattatccc ctctacgacg acgaaggtgc agtcatcggc ggcctttgcg tggttcgcga
      961 catcaccgat cagcggcacg cttacgaact ggcgcacgaa gcgcaccaaa gactaacttt
     1021 tcacgtccag aattcccccc ttgccgtagt cgagtgggat agcgactttt gtgtctcgcg
     1081 ctggtcggag tctgcggaac gactctttgg ttggaaggcg gaagaagttc tcggcaaaca
     1141 cgtcacggac tggcattttg ttttcgctga cgacgtgaat gacgtggtgc agttgaccag
     1201 caaacagcgc gaagggtcag aaacacgcgg ggtattgcgc aaccgaaact acacgaagga
     1261 cggatcgatt gttcactgcg aatggtacaa ctctgtgctt aatgacgatg agggcaggct
     1321 ggtatcggtg ctgtccctcg tgctcgacgt aactgctcgt gagctagccg cccaggagcg
     1381 cgccaaggcg ctgttgcgag aacgcgaggc gcgagaagag gcagagaaag cggatcgcct
     1441 caaagatgaa tttctcgcaa ctttgtctca tgaactccgc acgcctctga cttccgtact
     1501 cggctgggcc acgctgattc gggcgggcga catagacgca gccaactatg ccatcgcgct
     1561 ggaaaccatc gaacgcaacg ctcgcctgca ggcaaggttg atcgatgatc tgctggatgt
     1621 ctcgcgcatt atcactggca atctgcatct cgagctgcgg ccgatcgact tggcgccggt
     1681 ggttgaagcg gcgcgcgacg cggtgcggcc cacggcggaa gccaagggca ttgaactccg
     1741 gacgtcattg gccgtcggcg agtgttcgat caaaggcgac tcgaatcgcc tgcgacaggt
     1801 cgtttggaat ctccttctga acgccatcaa gttcacgccc cgtggcggca gcgtggccac
     1861 gaggctgcac tgtcgcaatt catcagtggt gttgaccgtg gaagataccg gggagggaat
     1921 tgaacccgac tttcttccgc atgtcttcga tcgttttcgc caggcggaag gctcgatctc
     1981 gcgacgccag ggcggcttgg gtttaggcct tgcagtagta cggcatctgg tggaattgca
     2041 cggtggcagc gtcagcgcgg ccagcgaggg aaaagggaaa ggtgcgactt ttaccgttga
     2101 gttgccactt gtaggacagg aacaagcatt tgtggcgagc aatgccgcgg ctacgctgac
     2161 gcgtgaaagc aattccacaa ccaccacagc cgccggcaag gattggcgcg tcaggaaggg
     2221 aaagaacaaa ccccaaacag ggaaagaaaa aaccgcagcg tc
//
LOCUS       DASUEX010000255         2245 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1082942, whole genome shotgun sequence.
ACCESSION   DASUEX010000255 DASUEX010000000
VERSION     DASUEX010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1082942"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="VFV61_04305"
     CDS             complement(<1..447)
                     /locus_tag="VFV61_04305"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HEX4899729.1"
                     /translation="MPLLKQGSTGPEVKTLQARLRALGFDPKGVDGKFGEDTRDALVA
                     FQISKGLPPDGRADPDTLRALGLTAGEAISAPATTVTTSVATPTSPTIGEPAAPPPNS
                     SPFAAASVSVVRKMFPDTPVANIQVNLPIVLQALADAGLADKEMVLM"
     gene            complement(601..>2245)
                     /locus_tag="VFV61_04310"
     CDS             complement(601..>2245)
                     /locus_tag="VFV61_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899730.1"
                     /translation="IINLNLFSLHNSYRSRLIRGFLAASRDKLLRQPNPFTGFDPADN
                     VSMHALRPALLHEVDFIDFDSFVNQFKDVAESVNREETTEPTTASQFIYTQLSEKVKA
                     EIDERDRDTPVSQGLRADLIEDLNRILEDEEINLSQHPGFSRPPQKPSDAPQSPDTKA
                     SAAVLQLEYHIHRNRRALELTYPQFIVESNYPPHRLLHVVNTTLNLVGGDNLAWQQRK
                     AEPFTISPLHCGSYRVGYRRSKDYGGDDGISLGTAMAISGAAASSNMGYYTTSPVLSL
                     VLTLFNVRLGWWLGNPGPAGSHSYRGLKTDEREDAASKKIYRRMSPTYSVLPVLYEAF
                     GLTDDRSDYVYLTDGGHFENLALYEMVLRRCALIVVSDGAADANYHFNDLANAVRKIR
                     IDLGVPIEFPDVPIYKEAPSKAGEGGCYWAVGLIRYSQIDRRNDGSNAKDGVLIYIKP
                     AVYGDEPEDVVHYKRTHPTFPNETTADQFFDEAQFESYRALGSYIMDRMCGSHSGAMS
                     RLEFASQVLQHLVKMAPQMKEKYVEFWADVAKEGREKTQ"
BASE COUNT          499 a          592 c          607 g          547 t
ORIGIN      
        1 catcaggacc atttccttgt cagcaagtcc agcatcagcg agcgcctgca atacgatggg
       61 gaggttcacc tgaatgttcg ccacgggcgt atcaggaaac attttgcgca cgacactgac
      121 actcgcagcc gcaaacggtg atgaattcgg gggcggagct gcgggttcac caatagtagg
      181 actcgtagga gtcgcaacgc tggtagtgac ggttgttgcc ggagctgaga tcgcctcgcc
      241 agcggtcaag cccaacgccc gcagcgtatc ggggtcggcg cggccgtccg gcggtaagcc
      301 tttgctgatt tgaaaagcca cgagggcatc gcgagtatct tcaccaaact taccgtcaac
      361 acctttcgga tcgaaaccca acgctctgag tctggcttga agtgttttta cctcagggcc
      421 ggtagagccc tgtttgagaa gaggcatgat tctccttccg tgttctcggg taaatttatc
      481 gtccgcagtt gagaaaagga cgcgcttgct tatcaacttg tttctagccg ggcttaatct
      541 atggaagctg ccatttttct gtggtgatca tcacctcaat cagacaactt tgagaaaata
      601 ctattgagtt ttttccctcc cctcttttgc gacatccgcc caaaactcca catacttctc
      661 tttcatttgc ggtgccattt tgaccaggtg ttggagtacc tgactggcga actccaagcg
      721 gctcatcgcg cctgaatgcg agccgcacat gcgatccatt atgtaagagc caagtgcccg
      781 atagctttca aactgcgcct catcaaagaa ctggtcagca gtcgtttcgt ttggaaaggt
      841 gggatgggtt cgcttgtagt gtacgacatc ttcgggttcg tcgccgtaga ccgcaggctt
      901 gatatagatc aagactccgt cttttgcatt agacccgtcg tttcgtcgat caatctgcga
      961 ataccgaatc aaaccaacag cccagtagca ccctccttca ccagccttgc tcggcgcctc
     1021 cttgtagatt gggacgtccg gaaactcgat aggtacgcca aggtcaatgc gaatcttgcg
     1081 caccgcgttc gccaaatcgt tgaagtggta gttcgcgtct gcggcaccat cgctgaccac
     1141 aatcagagca caacggcgca gtaccatctc gtacaaggcc aggttctcga agtggccacc
     1201 gtcagtcaaa taaacataat cactccggtc gtctgtgagt ccaaaggctt catagaggac
     1261 gggtaaaacc gaatatgtgg gagacatacg gcgatagatt ttcttcgacg ccgcatcttc
     1321 ccgctcgtcg gttttcagtc ctcggtatga atggctgcct gctggtcccg ggttgccgag
     1381 ccaccatccc agccgcacat tgaagagcgt caagacgagc gacaggaccg gagaagtggt
     1441 gtagtagccc atgttggagc tcgcggcagc acccgagata gccatggcgg ttcccaggga
     1501 aataccgtcg tcacccccat agtcttttga tctgcgatag cccacgcgat agctgccgca
     1561 atgtaacggc gagatagtga acggttcagc ctttcgctgt tgccaagcga gattgtctcc
     1621 cccgaccagg ttcaaggtag tgttcaccac gtggagcaat ctgtggggcg ggtagtttga
     1681 ttccactatg aactgcgggt aagtcagttc cagcgcccgc cggtttcggt ggatgtgata
     1741 ttcaagttgg agcacggctg cggaagcctt agtgtccggt gattgcggtg catcggacgg
     1801 cttctgcggt ggccttgaga atccgggatg ctgacttagg ttgatctcct cgtcttccaa
     1861 tattcggttc aggtcttcaa ttagatcggc acgaaggccc tgcgaaaccg gcgtgtctcg
     1921 atctctctcg tcgatttcgg ctttaacttt ctccgaaagt tgggtgtaaa tgaattgcga
     1981 cgcagttgtg ggctcagttg tttcttccct gttaacagat tcggctacgt ctttgaattg
     2041 attaacaaaa ctgtcgaagt caataaaatc aacctcatgc agcagggctg gtcggagtgc
     2101 atgcatcgac acgttgtcgg ccggatcaaa gcccgtgaac gggttcggtt gtctcaagag
     2161 cttgtcgcga gacgccgcca gaaagccgcg aatcaaacgg ctgcggtagc tattgtgaag
     2221 cgaaaagagg ttgaggttaa taatc
//
LOCUS       DASUEX010000256         4664 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1289446, whole genome shotgun sequence.
ACCESSION   DASUEX010000256 DASUEX010000000
VERSION     DASUEX010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4664
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1289446"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="VFV61_04315"
     CDS             complement(<1..523)
                     /locus_tag="VFV61_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899731.1"
                     /translation="MAENFSEQLDQALAVLMANPDARPPVAGEVESLLEIARELRRLP
                     REQFKSSLREQLLRGVAMSDAMNDASAAAVKKVNFIREGFHTITPYLAVHDVMGLIEF
                     VKQAFAAEGAVYGIGSQGGYHSEYKIGDSFVMIGGGGDWKGEQKLTALHVYVDDVDAS
                     YQRAVAAGAKAVMA"
     gene            complement(530..1093)
                     /locus_tag="VFV61_04320"
     CDS             complement(530..1093)
                     /locus_tag="VFV61_04320"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX4899732.1"
                     /translation="MVVGKPASTSAAELAADRLLVESAQKDQRKFADLYDKYFCLVYA
                     YTARRVGDRATAEDLTSEVFQKALEYLPKFVWRGVPFGAWLLRIATNIVVDRTRQAAR
                     EQHLSAQEDSSEISHFSLEEVEERASLFRSVDRLPEDQRRVIVLRFAEEKSIREIAAE
                     LGRSEGAVKQLQFRGLQTLRAELQAKR"
     gene            1237..1881
                     /locus_tag="VFV61_04325"
     CDS             1237..1881
                     /locus_tag="VFV61_04325"
                     /inference="COORDINATES: protein motif:HMM:NF019533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HEX4899733.1"
                     /translation="MKVNRGIVPLVFCVISFTGLFATPAFGQLGIGDPVEFRAYRDME
                     FRDPKRSPLETWDVPRFKGLNYFEINPAFKVKARFVRTPNEKKFNMPTSSGMTKVYVK
                     YAEIRFSLSGAEHVLGVYQSEASSQTAKYKNYLLIPFTDLTNGKESYGGGRYIDFEIP
                     SSELVTLDFNLAYNPSCAYSSRFNCPIPPRENKLKTKIRAGEKVYTKSHKAQSH"
     gene            1983..3896
                     /locus_tag="VFV61_04330"
     CDS             1983..3896
                     /locus_tag="VFV61_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899734.1"
                     /translation="MMIKPRIMNLFLPVLLLMAPSARGQEATPTSTADEQQQEKIEKQ
                     KRAFVLLEQVVDEAQLLRLPENRVRMQIGAAELLWQGNEGRARSLFSQAAEGVAEMMR
                     ATNPEAGSNERRNFNQSRTPIQLRQELVLTAARYDAPLAYQLLAATRPAVTDLAATGS
                     NLDDDLEQRLLAQVAALDPKLALQTAEDLLAKGKYPGSLLDVLNQLRTKDKEAAAKLE
                     DKLLKRLVAANMLTTADAGSLALALLQTGPRVANAAAADVSPAKNQPYLAATGYADLM
                     GSVIDAALKATPQSTTNQQRQNRGRGRSGFGAANQANANNTLTPAEVEQVNARRLLSG
                     LQMLLPRVEQTLPSRALAVRQKISELGLNNNQRANINQLLNNLDQQNSAALMASAAAA
                     PAGVQSRIYRRAALKALDEGNPELARQIAADHLEGNTRDSILQEMELRQLTKKAEGEA
                     IEEARRLAASLPNDEQRVDMLLQLAGRSQAANPKLALQLLEEARQITNKRATSYEQLD
                     LQLRVADAFKDIEPDRSFEIIEPGIMQLNELLAAAATLNGFEVNVFRDGEMPFQGGNG
                     LSAMVARYGQRLGALATKDFERAQTLANRFQLSEPRIVARLAIVRSLLGVEPAVNVNG
                     RRFRQEAFTRRGQ"
     gene            complement(3903..>4664)
                     /locus_tag="VFV61_04335"
     CDS             complement(3903..>4664)
                     /locus_tag="VFV61_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899735.1"
                     /translation="GVVWSIKLGEEAQSETVATRLVWAVGYFAEEAYYFENVRIRKLP
                     KHLSRGDEHVAPGNVLVGVRFEPRRSHIERHDPWDWDKNPFKDTEHLSGLKILMILLN
                     NFDARTANNRILYDKRTSSREALYYVTDLGATLGRAGGLGGKRTKNNLEDFLSTRFVM
                     GIEDGAVEFDYDTRPSKLGHLSILYPPYYIGEVKKEKSMRGIPVEHARWIGGLLSQLT
                     DEQLHDAFRAANYAPEIREGYVRSLRMRIDQLARL"
BASE COUNT         1135 a         1322 c         1211 g          996 t
ORIGIN      
        1 gagccataac ggctttcgcc ccggcagcca ctgcacgctg gtaacttgcg tctacgtcat
       61 cgacatagac gtgaagcgct gtcagcttct gctctccttt ccaatcgccg cctccgccga
      121 tcattacaaa agaatcaccg atcttgtact cggaatggta tccaccctgt gacccaattc
      181 cgtaaacagc gccttcggca gcgaatgcct gcttaacgaa ctcgatcagt cccattacat
      241 cgtgaaccgc gaggtaaggt gtaatcgtgt gaaagccctc gcgaatgaaa ttcacttttt
      301 tgaccgcggc ggcactggca tcattcatcg catcgctcat cgcaacaccc ctcagcaatt
      361 gttctctcaa gctagacttg aactgttcac gtggcaggcg ccgcaattcc ctggcgatct
      421 cgagcagcga ctcgacctcc cccgcaacgg gaggccgcgc gtcgggattg gccattagaa
      481 cggccagagc ctgatcaagt tgttcgctaa aattctcagc catgcttact caccgttttg
      541 cctgcaactc cgcacgcagg gtttgcagcc cacggaattg caactgtttt actgcgcctt
      601 cgctgcgtcc cagttctgct gcaatttcgc gaatgctctt ctcctcagca aagcgcaata
      661 caatcacgcg acgttgatct tctggaagac ggtccactga gcgaaacagc gacgctcgtt
      721 cctcaacctc ctcaagacta aaatgactga tttcgcttga gtcttcctgg gcagagagat
      781 gctgctcgcg ggcagcttgc cgagttcgat cgacgacaat gttggtagcg attcgtagca
      841 gccaggcgcc aaacgggaca ccgcgccaaa caaacttcgg caggtactcg agagctttct
      901 gaaaaacttc tgaagtgaga tcctccgcgg tggcgcgatc acccacacgg cgtgcggtgt
      961 acgcataaac caggcagaaa tacttgtcgt aaagatcggc aaactttcgc tggtcttttt
     1021 gcgcagactc caccaacagg cggtctgccg ccagctctgc cgcgctcgtc gatgctggtt
     1081 ttccgacgac cattgttctc taatgtataa actgtccagg ggtgaaaagg ttacgcagaa
     1141 ttttgcgccc cgctccggaa acggtcaaca agccgcctct gtacaaatgc agagcgatta
     1201 gtaagagcag ccaaagaaat aggagaaatc acgatagtga aggttaatcg agggatcgta
     1261 ccacttgttt tctgcgtaat aagctttacc ggcttatttg cgacgccggc ttttggacag
     1321 ctgggtatcg gcgatccagt tgagtttcga gcttaccgag atatggaatt tcgagatcca
     1381 aagcgatcgc ctttggaaac ctgggatgtg ccgcgcttca aagggctgaa ctactttgag
     1441 atcaatccgg catttaaagt taaggcgcgc tttgtgcgca ctccaaacga aaagaaattc
     1501 aacatgccga catccagcgg gatgacgaag gtatacgtga agtacgcaga aattagattc
     1561 agcctttcgg gtgcggaaca tgttctgggt gtttaccaaa gcgaggcctc gtcgcaaaca
     1621 gcaaaataca aaaactacct gctgattccg ttcactgatt taaccaacgg caaggaatcc
     1681 tatggtggtg gccgttatat cgattttgag attccatcca gcgaactggt tacactcgac
     1741 ttcaatcttg cctacaatcc gtcctgtgcc tatagcagtc gctttaattg tccgatcccg
     1801 ccgcgggaga acaaactgaa gacgaagatc agagccggag aaaaggtcta cactaagtcc
     1861 cataaggccc aatctcatta atctgttatg atcacccaac tctttagcgc cttcgggcgt
     1921 caaaagatct caactctctc agcaacgccg gccagcgttg tagcttcaca aacggagtag
     1981 caatgatgat taaaccccgg attatgaatc tgtttttgcc ggttctactg ctgatggcac
     2041 cgagcgcgag agggcaggaa gccaccccga cgtccactgc tgacgagcaa cagcaagaga
     2101 aaatcgaaaa gcaaaagcgc gctttcgtct tattggagca ggttgtcgat gaagcgcaac
     2161 tcctgcgctt acccgagaac cgcgtccgca tgcaaatcgg ggcagcggaa ttgttatggc
     2221 aaggaaatga gggtcgagcg cgctctctgt tttctcaggc tgctgaaggc gtcgcagaaa
     2281 tgatgagagc gacaaatccg gaggccgggt cgaatgagcg gcgaaacttc aaccaatcgc
     2341 gaactccaat tcagctacga caggaactgg ttctcaccgc cgcacgctac gatgccccac
     2401 tcgcttacca gttgcttgcg gcgactcgcc cagcagtgac agatcttgcg gctaccggtt
     2461 ctaacctcga cgacgatttg gaacagcgac tactggcgca agtcgcagcc ctcgatccaa
     2521 aactcgcttt acagaccgcc gaagatttgc tggctaaagg taagtatccc ggctcgctac
     2581 ttgatgtcct gaatcaattg cgaacgaaag acaaagaggc tgctgccaag ctggaagaca
     2641 agttattgaa acggctcgtg gccgctaaca tgttgacgac cgcagatgcc ggcagtttgg
     2701 cgttggcctt attgcaaact ggaccgcgcg tggcgaatgc tgccgcggcc gacgtttcac
     2761 ctgccaaaaa tcaaccctac ctggcagcca ccggttatgc cgacttgatg ggttcggtga
     2821 tcgatgcggc gttaaaagca acgccgcaat cgacaactaa tcagcagcgt cagaatcgcg
     2881 ggcgcggccg gagtggcttt ggtgccgcca accaggccaa cgccaacaac accttaactc
     2941 cagccgaggt cgaacaagtg aatgcgcggc gcctgctcag tgggctgcag atgctgttac
     3001 cgcgagtgga acagacgctc cccagccgcg ccctggccgt gcgccaaaaa atttccgagc
     3061 tcggcctgaa caacaatcag cgcgcgaaca tcaatcagct tcttaacaat ctggaccaac
     3121 aaaactccgc agccctgatg gcgtccgctg ccgcagcgcc cgctggagtt cagtcgcgca
     3181 tctaccggcg cgctgcgctc aaagctcttg acgagggcaa tccggagctg gcgcggcaaa
     3241 ttgctgctga ccaccttgag ggcaacacca gggattccat cctgcaggag atggagcttc
     3301 ggcaactgac aaagaaagcc gaaggcgagg ccatcgagga agcgcgccgt ctcgccgcca
     3361 gcttgccaaa cgacgagcag cgcgtcgaca tgctgctgca gttggcgggc aggagccaag
     3421 cagcgaaccc gaaacttgcg ctgcaattgc tcgaggaagc acgacaaata actaacaagc
     3481 gtgcgaccag ctacgaacaa ctagacttac agttgcgagt ggcagacgct ttcaaagata
     3541 tagaaccgga tcgcagcttt gaaataattg agccaggcat catgcaactc aatgaactgt
     3601 tggctgccgc cgcaactctc aacggatttg aagtaaacgt gttccgagac ggcgaaatgc
     3661 cgttccaggg tggcaacgga ttgagtgcga tggtggcacg ttatggccaa cgactcggag
     3721 ccctcgcgac caaggatttc gaacgcgccc aaactctcgc aaatagattt cagctttcgg
     3781 aaccacgcat cgtggcgcgc ctcgcgattg tgcggagcct gttgggagtc gaacccgcgg
     3841 tgaacgttaa cggacgtcgt ttccgtcagg aggcgtttac gcgccgcggt cagtagcaga
     3901 ggctagaggc gcgcaagctg atcaattcgc attcgcagtg agcgcacata gccctcccga
     3961 atttccggcg catagttcgc agcacgaaag gcgtcgtgca actgttcatc tgtgagctga
     4021 ctcagcagcc cgccgatcca gcgcgcgtgc tcaaccggaa tcccacgcat cgacttttcc
     4081 ttcttcacct caccgatgta atacggcggg tagaggatgc tgagatgtcc gagcttggac
     4141 ggccgagtgt cataatcaaa ttccactgcg ccgtcttcaa ttcccatgac aaagcgagtc
     4201 gaaagaaaat cttcgagatt gtttttggtc cgcttaccgc ccaagccgcc cgcacgaccc
     4261 agcgtggcgc cgaggtcggt cacgtaatag agcgcctccc tggagctggt acgtttgtcg
     4321 taaagaattc gattgttagc cgtacgcgca tcgaaattgt taagcaagat cataaggatc
     4381 ttcagcccgc taagatgttc cgtatctttg aatgggttct tatcccaatc ccagggatcg
     4441 tggcgttcaa tgtgcgaacg tcgaggttca aaacgcaccc cgactaaaac atttcccggc
     4501 gctacgtgtt cgtctcctcg tgaaaggtgc ttcgggagtt ttctaattct gacgttctca
     4561 aagtagtagg cttcttccgc aaaatatccc accgcccaga caagacgcgt cgcgacagtt
     4621 tcggattgtg cttcttcgcc cagcttgatg ctccagacca ctcc
//
LOCUS       DASUEX010000257         2848 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1083181, whole genome shotgun sequence.
ACCESSION   DASUEX010000257 DASUEX010000000
VERSION     DASUEX010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2848
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1083181"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="VFV61_04340"
     CDS             <1..187
                     /locus_tag="VFV61_04340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899736.1"
                     /translation="YEKRIASAKARPATVTSLESAILSHFHRLLSAEDVAKVLKALTA
                     AGHVVVSGKKVTYTDRG"
     gene            284..844
                     /locus_tag="VFV61_04345"
     CDS             284..844
                     /locus_tag="VFV61_04345"
                     /inference="COORDINATES: protein motif:HMM:NF024769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HEX4899737.1"
                     /translation="MKTFVAQTVDQWRKWLDKHHDSESEVWLIFHKIHTGVRSIEYLD
                     ALDEALCFGWVDSLVKRLDESRYARKFTPRKADSRWSTINRKRYAALKAAGRLKPGGI
                     DRPPTDRSYGPRPPRFQMPATVPGYIQAALTKQPAAWQYCEGLAPSHRRRYVGWIESA
                     KREETKVRRLQEAIRLLKAGKPLGLK"
     gene            940..1467
                     /locus_tag="VFV61_04350"
     CDS             940..1467
                     /locus_tag="VFV61_04350"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX4899738.1"
                     /translation="MQSALDQRDSLLDAWRSNNRVTVFLVENLPQDLWGATVPGAPRR
                     TVRMIAGHIHNARCMWIKTLGKEFGITVPRAINRHKVGPQELIPALGHSSRGIINLLK
                     LGFDHGGSIPVSSAYTWRNLPLDVGHVLTYFVAHEGHHRGQIVMLARQLGYRLPVEIT
                     GGLWQWTKRAKESRS"
     gene            1557..2057
                     /locus_tag="VFV61_04355"
     CDS             1557..2057
                     /locus_tag="VFV61_04355"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEX4899739.1"
                     /translation="MIWLRALFFVVAVQLTVIGVIPWLLSGVGPRVPPGNWSLVGIIP
                     LTIGALVLLWCNWAFVMKGRGTAAPYDPPVTLVAQGLYRHVRNPMYVSAVLIVLGTGF
                     LTGAAILFGYALLLAVSYHLFVRYYEEPHLQREFGTSYADYCAGVPRWWPRVTPWRGR
                     DSAPHT"
     gene            2124..>2848
                     /locus_tag="VFV61_04360"
     CDS             2124..>2848
                     /locus_tag="VFV61_04360"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX4899740.1"
                     /translation="MRKQNSILPLALLLGLVISSSAAQTSVVAGHWEGVMVREGAELP
                     VSFDFMNQAAGLAASFNSPTQRALGIPLRNVSYSAPKVHFELVGDATTIVFDGQLSRD
                     IIVGEFREGDARGTFSLRRVNAKPPTFKQEEVSFRNGDVTLSGTLLLPLTKGPHPGVV
                     FLHGAGAEGRYGARFLAEYFTRYGIASLIYDKRGVGKSTGDWQRSDFNDLAADAIAGI
                     HLLQQRREINPKQIGLYGHSQGG"
BASE COUNT          596 a          780 c          827 g          645 t
ORIGIN      
        1 ctacgagaag cgcattgcat cagccaaggc tcgacctgcg acggttacgt ccctggagag
       61 tgcaatcctt tcccacttcc acaggctgct ctcggcggaa gacgtcgcta aggtgctaaa
      121 ggcgcttact gcagccggcc acgttgtggt aagtggaaag aaggtcacgt atacggaccg
      181 cggctaacaa gcgtttggag cggactcgcg atggacgact ttattaagtt gcggggcgag
      241 ccgcttaagt tacgcgttgc gtggacaccg aagatcgcaa accgtgaaga cattcgtcgc
      301 tcagactgtt gaccagtggc gcaaatggct cgacaagcac cacgactccg agtccgaggt
      361 gtggctgatc ttccataaga tccacacggg cgtgcgatcg atagagtacc tggacgcact
      421 cgatgaggcg ctgtgcttcg gttgggtgga tagcctggtc aaacgcctgg acgaaagtcg
      481 ttacgcgcgc aaatttacgc cgcggaaagc ggatagccgg tggtcgacga ttaaccgcaa
      541 acgctacgcg gccttgaaag ccgctggcag gttgaagcca ggtggaattg atcgcccgcc
      601 aacggaccgc agctatggtc cgcgaccgcc acggttccaa atgcccgcca cggttccagg
      661 gtacattcag gcagcattaa caaaacaacc ggcggcatgg cagtactgcg aggggctggc
      721 accttcacat cggcgacggt atgtcggctg gatcgaatcc gccaagcgcg aggagacaaa
      781 ggtcagacgt ttgcaggagg cgattcgcct tcttaaggca ggcaaaccgc tgggactcaa
      841 gtaaacagcc gcctaacaaa ggcatgcaca ccgttagacc ctagctcgga ttgtctcctg
      901 ctcaacttgt ttggctcgat ctttctttga agtgttgtaa tgcaatccgc acttgaccag
      961 cgcgatagcc tgcttgacgc atggagaagt aacaaccgcg tcacggtctt cctcgtcgag
     1021 aacttacctc aggatttatg gggcgccaca gtgcccggtg ctccacgacg aacggtgcgg
     1081 atgatcgccg gccatattca caatgcccgg tgtatgtgga tcaagactct gggaaaggaa
     1141 tttggcatca cggtgccgcg agccatcaat cggcacaaag tcggaccgca agagctgatt
     1201 cccgcactag gccacagtag ccgcggcatt attaatttgc ttaagctcgg ctttgaccat
     1261 ggcggttcaa tccccgtttc ctccgcgtat acctggcgca atcttcccct cgatgtcggg
     1321 catgttctaa cttacttcgt cgcgcacgaa ggtcatcatc gcgggcagat tgtcatgctc
     1381 gcccgccaac taggctaccg tttgcccgta gagattacgg gtggtctttg gcaatggacg
     1441 aaacgcgcca aggagtcgcg gagctaagtg actgatggct tcgtctattc aatgatcgtc
     1501 gtgaacaatc agttgtcgac cgatccttga agcgttaact gcttggagat cgtaagatga
     1561 tctggcttcg cgcattgttc ttcgtagttg cagtgcagct cacagtgatc ggtgtcatcc
     1621 cctggttgct ctcaggtgtc gggccgcgtg ttcctccagg caactggtct ttggtcggta
     1681 tcatccctct gacgatcgga gctctcgtct tgttgtggtg caactgggcg ttcgtgatga
     1741 aggggcgggg cacggccgct ccgtacgatc cacccgtcac cctggtggcg cagggactct
     1801 atcgccacgt acgaaatcct atgtatgtaa gtgctgtgct aatcgttttg ggcactggct
     1861 tcttgaccgg cgccgcgatt ctctttggct acgcccttct gttggcggtc agctatcatc
     1921 tcttcgtgcg ctactacgag gagccgcacc tgcagcgtga gttcgggacg tcatatgctg
     1981 actactgcgc tggagtgcca cgctggtggc cgcgcgtaac gccgtggcgt ggccgggatt
     2041 ccgctccgca cacctgacga gctgttactc ccgacgcctc gcccccgtct atcgaatcgt
     2101 tatccagagt tgagaaccag cttatgagaa agcaaaattc aattttgccg cttgctctct
     2161 tgctcggact cgttatctcg agttccgcgg ctcagacgtc agttgtggca ggtcattggg
     2221 aaggggtgat ggttcgagaa ggtgcggagt tgccggttag ctttgacttc atgaatcagg
     2281 ccgcaggact ggccgcaagc ttcaactctc caactcagcg ggcgctggga ataccactgc
     2341 ggaacgttag ttacagcgcc ccgaaagttc acttcgaatt ggtgggtgac gctacgacga
     2401 tcgtcttcga tggtcagtta agccgagaca ttatcgttgg cgagtttcgg gaaggtgatg
     2461 ccagaggtac gttctcttta agacgagtga acgcgaagcc gcctacgttt aagcaagaag
     2521 aagtcagctt ccgtaacgga gatgtaactc tgtcgggcac gctactgctt cctttaacaa
     2581 aaggccctca tccgggtgtt gtatttctcc atggcgccgg cgccgagggt cgctacggcg
     2641 cgcgctttct cgccgagtat ttcacgcgct acggcattgc gtcgcttatt tacgataagc
     2701 gcggtgtagg caagtcaacc ggggactggc agcgttcaga tttcaacgat ctggcggcag
     2761 atgcgattgc cgggattcac ttgctgcaac agcgccgcga gatcaatccc aaacaaatcg
     2821 ggctttacgg acacagtcag ggaggcat
//
LOCUS       DASUEX010000258         2925 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_153813, whole genome shotgun sequence.
ACCESSION   DASUEX010000258 DASUEX010000000
VERSION     DASUEX010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2925
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153813"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..938)
                     /locus_tag="VFV61_04365"
     CDS             complement(15..938)
                     /locus_tag="VFV61_04365"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX4899741.1"
                     /translation="MPPKSNVLIVDDDPDKLYLLETALRLAGYETRAAASGEEALAAI
                     ATYQPDLVISDVMMPGLNGYDLAREIRLNPRTKFIPIILQTAARFQAEDLLRGSEAGA
                     LGFITDPSDLDLLLARARTLLEFKAYLDTCEEAAFTDHLSGLANRRRFERQLEREVER
                     TLRYRRPFCLLLLDIDNFKQVNDSYGHEAGDQVISRIARSLREGTRGIDLAARIGGDE
                     FGIILTETAIASAVDVAERLRQSIYSVKISDASITGSFGIAECWSNGENARDLLSAAD
                     AALYRAKRLGRNRIEQADNSKSNSVAAGDVQ"
     gene            1191..1943
                     /locus_tag="VFV61_04370"
     CDS             1191..1943
                     /locus_tag="VFV61_04370"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX4899742.1"
                     /translation="MRDQENRGIQSKRAHDVSVEDRAQLIRKFEFGSTLSDASVLALA
                     QTSTLVDVRRRRSVYRAGDAADTLFGIVQGRIKLCRVEKGTNREAVIDILPEGSLLGE
                     SALYSTAGQRENSAVAYEHSRLLRIPVGDFKRGMAEDDKLHDYTFRLIGQRLENAERR
                     LADFTLNAIPARLDRLLVDFSNRYGVQEQEGVLIDISLPHREIASIVGSTRESVTVRL
                     NAMVREGTIKFVNRRILVKRLETLTGEVSVAN"
     gene            complement(2152..>2925)
                     /gene="lpxI"
                     /locus_tag="VFV61_04375"
     CDS             complement(2152..>2925)
                     /gene="lpxI"
                     /locus_tag="VFV61_04375"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF017989.4"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HEX4899743.1"
                     /translation="SLSIAAIREETDPQIEQLADRLTWVGIGQLGKMIRFFKDEGVEQ
                     AMMAGQVRHIQIFSRAVPDARMLKMLLRLPRRNTHSLIGAVAEELASEGIQLIDSTYF
                     LRESLPQAGTLTKRQPDSRERQDIEYGLEIANEIASLDLGQTIVVRGRACVAIEAMEG
                     TDETIRRAGQLTGGARPRGIMRGRRISLSVGQLTVVKIAKPDQDMRFDVPVVGVPTIE
                     AMIEAGATCLCISAGKTLMFDRDEMIALADKKRIAIEAI"
BASE COUNT          669 a          813 c          760 g          683 t
ORIGIN      
        1 ctgagacttt ccacctactg tacgtctcct gccgccacag agttcgactt gctgttgtcg
       61 gcttgctcga tccggtttcg acccagcctt ttcgcccggt aaagtgcagc atctgccgcc
      121 gagagcagat cgcgtgcatt ctcaccatta gaccagcatt ccgcaattcc aaaactcccg
      181 gtaatgctcg catccgatat cttgacggag taaatcgact gccgaagtcg ttcggcaacg
      241 tccactgcac ttgcgatggc ggtctcagtc aagatgatcc cgaactcgtc ccctccgatt
      301 cgcgccgcca agtcgattcc acgcgtacct tcacgcaggc ttctggcgat ccggctgatt
      361 acctggtcgc ctgcctcgtg gccataggag tcgttaacct gcttgaagtt gtcgatgtcc
      421 agcagcagca agcagaaagg tcgccgatag cgcagggttc gttcgacctc ccgttccagt
      481 tgccgctcaa accgcctacg atttgccaaa ccggacagat ggtcagtaaa cgcagcctct
      541 tcacacgtat ctaggtaggc cttaaattca agcagcgttc gcgcccgcgc gagcaacagg
      601 tcgagatccg agggatcggt aatgaagcca agcgctcctg cctcagaacc ccgcaataga
      661 tcctcagcct ggaagcgggc cgcagtctgc aggatgatgg gaatgaactt ggtacgcgga
      721 tttagccgga tctccctggc caggtcgtaa ccattgagac ctggcatcat gacgtcagaa
      781 atcaccaaat ccggctggta agtggcaatc gcagcgagcg cctcttcgcc ggacgcggcc
      841 gcacgggtct cataccctgc aagccgcaga gcggtctcca ggaggtacag tttgtcagga
      901 tcatcgtcaa cgatcagaac gttagatttt ggtggcataa gttcaattta attgttcaga
      961 ataggtgctt gcgcgcgcgg attcttcatg ctaaagtttg gaccattcag tcattgtccg
     1021 ttttcgttta aagcacatcc caaccgccga atccgtacaa taattggaat aatcaagacc
     1081 tcgggtattc accagggctt ccctacacta cattcaatca cattgctcga taaaggttgt
     1141 cgcgtcagtc aaatccaggc ccaactcata acttgaaggt tcaatctccc atgagggacc
     1201 aggaaaacag agggatacaa agtaagcgtg cccatgacgt gagcgtcgaa gaccgcgccc
     1261 agttgattcg aaagtttgaa ttcggctcca ctctaagcga cgcatccgtt ttggcattgg
     1321 cccaaacttc cacccttgtc gatgttcgcc ggcgacggtc tgtctatcgc gctggcgatg
     1381 cagccgatac gctcttcggc attgtccagg gcagaataaa actctgccgc gtagagaaag
     1441 gaaccaatcg ggaggcagtc attgacatac ttcccgaggg ttcgcttctt ggcgaatcgg
     1501 ctctttattc aactgcgggg caacgcgaaa actcggcggt tgcttacgag cactcgcggc
     1561 ttttacgaat tccagttggg gatttcaaac gggggatggc tgaggacgac aagcttcatg
     1621 attacacttt ccgtttgatc ggtcaacgac tcgaaaatgc tgagcgcagg ttggcggatt
     1681 ttacgctgaa tgcgattccc gcacgcctcg ataggctact ggtggatttc tccaaccgct
     1741 atggcgtgca ggaacaggag ggcgtgctaa tcgacatttc tctgcctcat agggagatcg
     1801 cttcgattgt gggatcaacg cgcgagagtg tgaccgttcg cttgaatgca atggtgcgag
     1861 aaggaacaat taaattcgtt aatcggcgca ttctcgtcaa gcgactggag acgctaactg
     1921 gtgaagtcag cgtcgcaaat tgagctaggg ggttttcagt taataccgtc cgatgaaatg
     1981 tcctacctgc ggtaaacctg ccgagtggaa agataatcct tttcgaccat tctgttctga
     2041 acgctgcaag ctagtggatt tgggtcgttg ggtgaatgag gaatacagag tgcccggggc
     2101 ccggctgcct tccgaggatt tggacaggca ggaaaaggag tctcatgaag attaaattgc
     2161 ctcaatggcg atcctcttct tgtccgccaa tgcaatcatc tcgtcccgat caaacatcaa
     2221 agtcttgccg gcactgatac agagacaggt ggccccggct tcgatcattg cttcgatggt
     2281 cggaacccct acaacgggca cgtcaaagcg catgtcctga tccggtttcg cgatctttac
     2341 aaccgtcaac tgcccgacgg agagactgat tcttctgccg cgcatgattc cgcgcgggcg
     2401 cgctccaccg gtcaactggc cagctctgcg aatcgtctca tccgtcccct ccatggcttc
     2461 aatagcaaca caggccctgc ctctcaccac aattgtctga cccagatcga gggacgcgat
     2521 ctcatttgcg atctcaagtc catattcaat gtcctgacgc tcacgactat ccggttggcg
     2581 cttggttaac gtaccggctt gcggcagaga ttcgcgcaga aagtaagttg agtcgatcag
     2641 ttgaatgcct tcactcgcaa gctcctcggc gacagctccg ataagagaat gagtattgcg
     2701 gcggggaagg cgtaacaaca tcttcagcat ccgggcatcg ggcacggccc tactgaatat
     2761 ctggatgtgc cttacctgcc cggccatcat tgcctgttca acgccttcat ccttaaaaaa
     2821 ccggatcatc ttgcccaatt gtccaatacc gacccaggtg agacggtctg cgagttgttc
     2881 gatttgggga tcagtctctt cacggattgc cgcgatcgaa agtga
//
LOCUS       DASUEX010000259         2326 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1420276, whole genome shotgun sequence.
ACCESSION   DASUEX010000259 DASUEX010000000
VERSION     DASUEX010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1420276"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..528)
                     /locus_tag="VFV61_04380"
     CDS             complement(<1..528)
                     /locus_tag="VFV61_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899744.1"
                     /translation="MQIQSLVPVSTTDAQVVAGVSPSNGAARKAASKPTVKPLPTLDD
                     LLATASLQARGWRGWLRVAQVGRVLGLLTLYIFLDSYDVRAKFNQRTADRLREATRDK
                     GRAARILVWLRELGIRVLDRAIRMLRYFVFRGTDGSVKKEARLAKQATWLREKLIDLG
                     PTFIKIGQALGTRGDL"
     gene            complement(547..1500)
                     /locus_tag="VFV61_04385"
     CDS             complement(547..1500)
                     /locus_tag="VFV61_04385"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004159.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEX4899745.1"
                     /translation="MRLFHGTENADIQRPTVLTLGVFDGLHLGHQLIIRTVAERAKAI
                     GAVPTVITFDPHPRAVLHPESSPPLLQTLDQKVEGFGILGIEQTIVIRFTPDFALIRA
                     EGFLRDVVKERLQAREVYLGRGFNFGHNREGNIDLLKRVSEDLGFVAGEVAEVRLRGQ
                     RVSSSKIRQLLAQGRVNLARRMLGRPYGVEGLVIRGNERGHTLGFPTANLHPQNRVIP
                     AHGVYVTGTLIDGQWRRSVTNIGTRPTFETATEPSVETFVMNWSGDLYGDVIRVRFLY
                     RLRDEKKFASLDDLRTQISKDVGQAGRYFERSGARHVLSIL"
     gene            complement(1690..>2326)
                     /locus_tag="VFV61_04390"
     CDS             complement(1690..>2326)
                     /locus_tag="VFV61_04390"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX4899746.1"
                     /translation="IVLVDTSSDFRQQALRHQLKRLDAVLITHCHADHIFGLDDIRPL
                     NFRYGALGLYANERAWPDIRRIFKYIFEPSHFGGGLPQVIPHTVFPNAEFCLGESLRI
                     IPLEVIHGRLPVMAYRFNDFAYATDLSEIPAATLAALTNLEVLVLDCLRFAEHPTHLW
                     LDKALEYIARIKPRRAYLTHIAHDVKHERDSARLPDDVAWAYDGLVVTSNE"
BASE COUNT          557 a          634 c          626 g          509 t
ORIGIN      
        1 tagatcgcct cgggttccga gggcctggcc aatcttgatg aaggtggggc cgagatcgat
       61 cagcttctca cgtagccacg tagcttgctt tgcgagccgc gcttcttttt tgacggaacc
      121 gtcggtcccc cggaaaacaa aatatctcag cattcgaatc gcgcgatcca gaacgcggat
      181 gccgagctcg cgcaaccaga ccaggatgcg cgccgcccga cccttgtcgc gggttgcttc
      241 acgcaatcga tctgcggttc gctgattgaa tttggcgcgt acgtcgtagc tatcgagaaa
      301 gatatagagc gtcagcaaac ccaacacgcg gccaacctga gcaacacgca gccagccacg
      361 ccatccccgt gcttgcaaac tcgcagtcgc cagcaaatcg tcaagcgtgg gcagcggttt
      421 cactgtgggc ttggaggcag ctttacgggc cgcaccattg gaaggtgaaa cacctgcgac
      481 tacttgcgcg tctgttgtcg aaactgggac cagggattga atctgcatct acgaggattc
      541 taaagtttaa agtattgata agacatgccg cgcgccggag cgttcgaaat atcttcctgc
      601 ctgcccaaca tctttgctta tctgagttct caaatcatca agcgacgcaa acttcttttc
      661 gtcacgcagg cgataaagga atcgtacacg aatcacgtcg ccgtaaagat cgcccgacca
      721 attcattacg aatgtctcta ccgaaggttc cgtcgccgtt tcaaacgtcg gtcgggtgcc
      781 tatatttgtt acgctacgac gccattggcc gtcaattaac gtccccgtga cataaacgcc
      841 atgggcaggt atgacgcggt tttgcgggtg caggttggct gtgggaaatc cgagggtatg
      901 gccgcgctcg ttgccgcgaa tgacaagccc ttccacccca tagggacgtc ccagcatgcg
      961 gcgcgcgagg tttacccggc cctgcgccag caactggcga atcttgctcg agctcactcg
     1021 ctggccccgc agacgaactt cagccacctc gccggccaca aagccgagat cctcgctgac
     1081 tcgttttagc aggtcgatat taccttcgcg attgtgacca aaattaaagc cgcgaccgag
     1141 gtagacttcg cgcgcctgca agcgctcctt gacgacgtcg cgcagaaagc cttcagcgcg
     1201 tatcagggca aagtctgggg tgaagcgaat tacgatcgtt tgttcgatgc cgaggattcc
     1261 aaaaccttca accttctgat cgagggtttg caatagtggc ggcgacgact cgggatgcaa
     1321 gacggctcgc ggatgcggat caaacgtgat cacggtcgga acagccccga tcgcttttgc
     1381 tctctccgcg actgtgcgaa tgatcagttg gtgtccaagg tggagtccat cgaatacgcc
     1441 cagggtcaac acagtgggcc gttgaatgtc agcgttttcg gttccgtgga agaggcgcat
     1501 caattggttg gaagagagcc agcaacgttc aaaagctcag ttatcatttt ccatttatca
     1561 ttttccatgt tccattttca gaccaatctt gtgatcctct tgaacgaaag ctactcaact
     1621 cgaagcgcaa atgaaaaatg agaaatggaa aatgatatat ggaaaatttc ctttcatgta
     1681 agctggtcct cactcattgc tggtaaccac aagtccatcg taggcccagg caacatcgtc
     1741 cggcagtcgc gcagagtcgc gctcatgttt cacgtcgtga gcaatgtgtg tcaggtaagc
     1801 acggcgtggt ttgatcctcg caatgtattc cagagccttg tcgagccaaa ggtgagtggg
     1861 gtgctcggca aaacgcaaac agtcgagcac cagcacctcg aggtttgtca gcgccgccag
     1921 cgtcgctgca ggaatctcgc tgaggtcggt agcataggca aagtcattga agcgataggc
     1981 catcaccggt agccgtccgt ggatcacttc caggggtatt atccgaagac tttcacccaa
     2041 acaaaactca gcgttgggaa acaccgtgtg ggggattacc tgcggcaaac cgccgccaaa
     2101 gtgtgaaggc tcaaaaatgt atttgaagat gcggcgaatg tccggccaag cgcgctcgtt
     2161 ggcgtaaaga ccaagtgcgc catagcgaaa atttagcggc cgaatatcat ccaggccaaa
     2221 gatgtgatcg gcatggcagt gcgtaattaa tactgcgtcc aaacgcttta actggtgccg
     2281 caaagcctgc tgacgaaaat ccgaagaggt atctaccagc acgatc
//
LOCUS       DASUEX010000260         3839 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1084220, whole genome shotgun sequence.
ACCESSION   DASUEX010000260 DASUEX010000000
VERSION     DASUEX010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3839
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1084220"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2743
                     /locus_tag="VFV61_04395"
     CDS             <1..2743
                     /locus_tag="VFV61_04395"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HEX4899747.1"
                     /translation="DSGKQLLKMNGRTNMAYGVAFSNDGNRLSSGGRTTWDLRTGRGL
                     RLAAAPIDQQLTIPSPDGKLLAVFSPTGNSVAIVETPSGRKLQSLTPAGQSVVQRASF
                     SADSNLLAVTYTASEAQMQEGKGPGQTGSNESPVRIWDVKSGRELRALNLGTSAAAAS
                     FSADGRLIATLGSMGESSLWDTASGNKLRDFTNSPMANITQMMQGAGGLTPDQLKKMK
                     PGKMGSMPQMPDLSAMTSMITNMMGSMSAGTMGRSVTSLAFSPDGRTLAIGGVESKSN
                     LDLAAMMDPAAQRKQKKNSKPTDPQEMMKNMKVESVGQVTLWDVATGSPSGTLSGHGK
                     GVTQVVFSRDGRLLASSSSDNSIRIWDVAARRELRSLKGHTANIESLDFSPDSRLLAS
                     ASDDGGTFLWDATTGEHLLTLISLDDGGEWMVVTPQGLFDGTPISWSQILWRYNQDTF
                     NVAPIEWFFNEYYYPGLLGEIFAGKRPKVTEDISRKDRRQPVVKLSLVDPAGATATGI
                     STRTTKVKIEITDAAADKDNSQSAGAQDVRLFRNGSLIKVWRGDVLGGKPAVTLEEEV
                     SVVAGPNQLTAYAFNRDNVKSKDSQLLLTGADSLKRAGTLYIIAVGVNEYANPQYNLK
                     YAVADAQSFAEEVRRRQMQIGQLERVEVVPLLNERATKTNILSALQQLSGIPGPPSLK
                     ATDPAFSGLKRAQPEDKVIIYFAGHGTAQAQRFYLIPHDLGYTGDRTKLNEQGLQKIL
                     ASSISDIELEQAVEGLDAAHLLLIIDACNSGQALEAEEKRRGPMNSKGLAQLAYEKGM
                     YILTAAQSYQAALEAAQLGHGLLTYALVEEGLKTAVADSEPQDGLLSAREWLNFATER
                     VPQMQEAKMKQSRGVGVQIAFTEGEEKVAEPEKRTVQRPRVFYRRELEANPLVIAKP"
     gene            2953..3702
                     /locus_tag="VFV61_04400"
     CDS             2953..3702
                     /locus_tag="VFV61_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899748.1"
                     /translation="MPFHFSARVPGRASCTILRVFCTLLLALLLQSISFNLQGGHTQT
                     QQRPSYKFDPLTPEERELALRLVEADARVKKLRGQGRQNLISVELATPKTGDQNDETS
                     RHAEILYYRYEGNQGVLVLVDLQQRSVQEAVAINGDAVPLVAEEVNQALSLALQNRTL
                     INLLGPNYQQYRVATQNSPEGEPNRVEALRLFASSPDDPCYRRRCVSLLFRQGDTFLT
                     GTEVIVDLTTQRVRVEQPAQRRPSTMRRRGR"
BASE COUNT          950 a         1051 c         1061 g          777 t
ORIGIN      
        1 cgacagcgga aagcagttat tgaagatgaa cggccgcacc aacatggcct acggtgtcgc
       61 gttcagcaac gacggcaatc gcctctcttc aggagggcgc actacctggg acctaagaac
      121 aggtcgagga ttacgactcg ctgcagcgcc aatcgatcag cagttaacca ttcccagccc
      181 cgacggaaag ttgctcgctg tgttttcccc aaccggtaac tcggtagcga tcgtggaaac
      241 gccttctggt cgaaaacttc aaagcctcac tcccgcaggc caaagcgttg tacagcgtgc
      301 cagcttcagc gccgacagta atctgctcgc ggttacttac acagccagtg aagcgcaaat
      361 gcaggaagga aagggtcccg gccagacagg cagcaacgag agtcccgtca gaatctggga
      421 cgtcaaatcc gggcgtgaac tgcgcgcgct taatctggga acgagtgctg cggcagcgag
      481 ctttagcgcc gatggccgct tgatcgcgac gctgggatca atgggagaga gttcgctgtg
      541 ggataccgca tcaggtaata agctgcgcga ttttactaat tcaccgatgg ccaacattac
      601 ccagatgatg caaggcgcgg gcggcttaac ccccgaccag ctaaaaaaga tgaagcccgg
      661 caaaatgggc agcatgccgc aaatgcccga tctatctgca atgaccagca tgatcacaaa
      721 catgatgggt tcgatgtcgg ccggcacgat gggccgcagt gtcacatccc tggctttcag
      781 tcccgacggt cgaacgctgg cgatcggtgg agtggaatcg aagtcgaatc tcgatctcgc
      841 cgcaatgatg gatccggcgg cgcagcgcaa gcaaaagaaa aactccaaac cgactgaccc
      901 ccaggaaatg atgaaaaaca tgaaggtcga gtcggtaggg caagtaactt tgtgggatgt
      961 agcgacgggt tctcccagcg gcacactcag tggccacgga aaaggggtta ctcaagttgt
     1021 gtttagtcgc gacggtcggt tgctcgcttc gagcagcagt gataactcga tcaggatctg
     1081 ggacgtagct gcgcgccgag agctgcgctc tctcaaaggt cacaccgcca acatcgagtc
     1141 gctggatttc agccctgact cgcgtttgct cgcttccgcc agtgacgatg gcggcacctt
     1201 tctttgggac gcgactaccg gcgaacattt attaacgctg atctcgctcg acgacggtgg
     1261 cgaatggatg gtggttactc cgcaaggtct gttcgacggc accccaatct catggagtca
     1321 gatcctctgg cgctacaacc aggacacgtt caacgttgca ccgatcgagt ggttcttcaa
     1381 cgagtactac tatcccgggt tgctgggaga gatcttcgcg ggcaagcgcc ccaaggttac
     1441 cgaggatatt tcgcggaagg atcgacgaca accagtagta aaactctcgt tagttgatcc
     1501 ggcaggcgcg actgcaaccg gaatttctac ccgcactacg aaagtaaaaa tcgaaatcac
     1561 tgatgccgca gccgacaagg acaactctca aagcgcggga gcgcaagacg ttcgcctgtt
     1621 tcgcaacggt tcgctgatca aagtctggcg tggcgacgtc ttaggcggga aaccagcagt
     1681 gacactggaa gaagaggtca gcgtggttgc gggaccgaac caattgaccg cctatgcgtt
     1741 taatcgagac aacgtcaaaa gcaaagactc acaattactg ctcaccggcg ccgatagctt
     1801 gaaacgcgca ggcacgcttt acattatcgc ggtgggggtt aatgagtacg ccaaccctca
     1861 gtacaacttg aagtatgccg tcgccgacgc gcagagcttc gctgaagaag tgcgccggcg
     1921 gcagatgcaa attggtcaac tcgaacgcgt cgaagtcgtg ccgttgctaa atgagagggc
     1981 aaccaaaacc aacatccttt cagccctgca acaactgtcg ggaattcccg ggccgccttc
     2041 attaaaagca acggatcccg cgttcagcgg attaaaacgc gctcagccag aagacaaagt
     2101 gatcatttac ttcgccggtc atggcacagc acaggcacag cgtttctatc tgatcccgca
     2161 tgaccttggc tatacgggcg accgtacgaa gttaaatgaa caaggcttgc agaaaatcct
     2221 ggccagcagc atttctgata ttgagttgga acaagcggtt gaaggtttgg atgccgcgca
     2281 tctcctgttg attatcgacg cctgcaattc gggacaagca ctcgaggcgg aagagaagcg
     2341 gcgcggtccg atgaattcca aaggtctggc ccaactggct tatgagaaag gtatgtacat
     2401 tctcacggcc gcccagagtt accaggcagc gcttgaggca gcgcaacttg gccacgggtt
     2461 attgacgtac gcactcgtgg aagaagggct gaagacagcc gttgccgata gtgaaccgca
     2521 ggacggcttg ttgagtgcga gggaatggct caacttcgct accgagcggg tgccgcaaat
     2581 gcaggaagcg aagatgaaac agagtcgcgg cgtcggcgtg cagatcgcgt ttactgaagg
     2641 cgaggaaaag gttgccgagc cggagaagcg caccgtgcag cgcccgcgag ttttctaccg
     2701 gagagagtta gaggcgaatc cgctggttat cgctaagcct tagtcttgta agtgcgcccg
     2761 gctgagtccc gacgagtcgg gaacgacggc gccttgaatc ggtgcacgcc tttacctcgc
     2821 aactaaacgg gtctatgctg ccagctagaa cacggtctct acgatctttc aatccaaacc
     2881 gatagtttct cctcgctcgc tatcgacttc acatcaacag ccgaaaggca cagctaaaag
     2941 ggaggtattc acatgccttt tcacttttca gctcgtgtgc cagggcgcgc ctcatgtacc
     3001 attctccggg tcttctgcac gctcctactg gcactcctgc tccaatcgat ttccttcaac
     3061 cttcaagggg ggcacaccca aacacagcag cgtccatcct acaagttcga ccctctcacg
     3121 ccggaagaac gcgagctggc tcttcgtcta gtcgaagctg acgcgcgtgt caaaaaactg
     3181 cgcggacaag ggcgtcaaaa cttgatctcc gtagaactgg caacaccaaa aacgggagac
     3241 caaaatgacg aaaccagtcg gcacgcggag attttgtact accgctacga aggcaatcag
     3301 ggggttttgg tactggttga cctgcaacag cgatcagtac aggaggccgt agccatcaat
     3361 ggcgatgcag tcccgctcgt tgccgaagaa gttaaccagg ctttaagcct cgcgctgcag
     3421 aacagaacat tgatcaattt gctcggtccg aactaccagc aatatcgcgt cgctactcag
     3481 aattctccag agggagagcc caatcgggtt gaggctttgc gactatttgc cagttcgccc
     3541 gatgatccat gttaccgacg tcgttgtgtc tcgctgctat tccggcaggg cgatactttc
     3601 cttactggga ccgaagtgat tgttgatctc acaacgcaaa gagtgcgcgt ggaacaacct
     3661 gcacaaaggc gtccctcaac aatgagaagg aggggaagat gaaatcaaga aacaaactca
     3721 ccatctgctt gttttcagtt ttcgccgttc tgtttgtatt ggctcagcct gctacagtgc
     3781 aggcccaggg atgctcgagt ccctttttca tcgagcagcg ctttcccgtc aacacttcc
//
LOCUS       DASUEX010000261         4033 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1421217, whole genome shotgun sequence.
ACCESSION   DASUEX010000261 DASUEX010000000
VERSION     DASUEX010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4033
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1421217"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..795)
                     /locus_tag="VFV61_04405"
     CDS             complement(46..795)
                     /locus_tag="VFV61_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899749.1"
                     /translation="MKAIGCKSVSKEIDELESGQLPGASTQAHLQACGACRSFYDDRQ
                     KLRQMLARLETVEAPGDFDFRLRARLANLRDERPAVFSSFGFGLPSVALAVLAILIGV
                     GLYLRATTDSVTQDPLARSEVTKTAGPAAVQPSSPADVKSNELTASASRENPEKTSIE
                     EPAAVVKEPVRSRKGNRSSNSNTIRRDDFLAKSETSAMFPLDAAEPLRVSVDYATGGS
                     RTISLPAVSFGSQQVVARGASMVKTSARTVW"
     gene            complement(806..1447)
                     /locus_tag="VFV61_04410"
     CDS             complement(806..1447)
                     /locus_tag="VFV61_04410"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX4899750.1"
                     /translation="METLSSIDELSFPTAVRQDVISDSTSDHALLEATRTGDQDAFAE
                     LVGRYRNQLTGYIYRMTNDYDGAVDLAQETFVRLYRAVDRYQTSHAFSTYLYRIATNL
                     AISELRKRKRRRLVSLTGFFQTTDRGEAREFDPADERPLIDCELVATERRQAVQRAIA
                     TLPEKYRAPLILRDVEGRSYEEIARILQTSEGTVKSRISRARDFLREKMKAYF"
     gene            complement(1643..2479)
                     /locus_tag="VFV61_04415"
     CDS             complement(1643..2479)
                     /locus_tag="VFV61_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899751.1"
                     /translation="MFRLFIAPAVFCAFVIGIIALAPTSGASSLNPQDRTSSVPTPTP
                     APVKVDKNTKTFTAEQIAESAIFIYGSRPVLQQIRRNGVERGRITRLANDGKMEEATY
                     ERVFVRGDTLDNDKIRLDQKMPTLEYSLIFGGGQLWGMINGAAFTPRQDAAAAFVSQH
                     RHSIEALLRYKETGATVKLIGKDKQKGLDLYVLDLIDKEQRTTRFYVSAKTLHILWLE
                     YEERGAKFSKRFADYRYAQSTLVPFRTILLEDGKQTQETRILTVTFGVKVDDALFKNP
                     SA"
     gene            2864..3955
                     /locus_tag="VFV61_04420"
     CDS             2864..3955
                     /locus_tag="VFV61_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899752.1"
                     /translation="MNRVGILVGREKTFPDALIKTINERGNGKTVAEFITLGGIRHDD
                     PPQYDVVVDRISHEVPFYRATLKRMALEGTVIINNPFWWSADDKFFNYSLAAKLGVAV
                     PRTVLLPQKDYIKGIVSESLRNLEFPLDWQGIVEYVGLPAFLKPFDGGGWKNVSKINS
                     IEDLWKEYDETGTLCMTLQAAVDFDQFVRCYCVGQEEVMIMAYDPRKPYLSGEQYIHH
                     PDYLEPALKDRVTKDVRTLCTALGYDLNTVEFAIKDGVPYAIDFMNPAPDAELASVGE
                     YYHNWIVGAVADLVFKRLAEPRQRARYRWDALLNPEPQSTAAVASPAEQQARTIVPTP
                     ATTEAPKAKTATAATARKKSSPSRSKPTA"
BASE COUNT          902 a         1124 c         1069 g          938 t
ORIGIN      
        1 gaatttgaat ggcttgtttc attgaactct gaatcacgtt cccgtttacc aaaccgtacg
       61 tgctgaagtt ttcaccatcg aagcgccgcg cgctacgact tgctgcgagc caaaacttac
      121 tgcgggcaat gaaatagtcc gcgaaccgcc agtcgcatag tcgactgaca cgcggagcgg
      181 ttcggcggcg tcgagcggga acattgccga agtttcactc tttgccagga agtcgtcacg
      241 ccggattgta ttgctgttgg agcttctgtt tccttttcga ctgcgcacag gttccttcac
      301 cactgctgcg ggttcttcga tgcttgtctt ctcgggattc tcgcgagacg cgctggcagt
      361 taactcgttg gacttgacgt cagcgggact gctgggttgg acagcagccg gcccggcggt
      421 ctttgtaact tcggaccggg caagtggatc ctgagtcacc gagtccgtcg tcgcacgcag
      481 gtaaagccca acgccgatca ggatcgccag caccgccaaa gcaactgaag gtaagccaaa
      541 accaaaactg gaaaatactg caggacgctc gtcgcgcaga ttcgccagcc gcgcgcgtag
      601 acgaaaatca aaatcaccgg gagcttcgac tgtttccaat ctggcaagca tttgtcgcaa
      661 cttctgccgg tcgtcataga aactgcgaca cgccccgcag gcttgcagat gcgcctgggt
      721 gctggcaccg ggcagctgcc cgctctccaa ttcatcgatt tctttactga cactcttgca
      781 accgattgcc ttcataagcc ctcacttaga aatatgcctt catcttctcc cgtagaaaat
      841 cgcgcgcgcg actgatccgc gacttcacag ttccctcgct cgtttgcagg atgcgggcta
      901 tttcctcata gctacgacct tccacatcac gcaaaatcag tggcgcacga tacttttctg
      961 gtagcgtggc gattgcacgt tgcaccgcct gccgccgctc tgttgccacc agctcgcagt
     1021 cgatcaacgg acgttcatct gccgggtcaa attccctcgc ttcgccgcga tcggtagtct
     1081 gaaagaatcc tgtcaatgaa accaacctgc gacgctttcg cttgcgcaac tcactgatgg
     1141 ccaggttagt ggcaatccgg tacaggtaag tcgaaaaagc gtggctggtt tgataccgat
     1201 ccaccgcccg atagagcctg acaaaagtct cctgcgccag gtccaccgcc ccgtcgtaat
     1261 cgttggtcat ccggtagatg tagccggtca actgattacg atagcggccc accaactcag
     1321 caaacgcatc ctggtccccc gtccgcgtcg cttccagcaa ggcgtgatct gaagtcgaat
     1381 ccgagatcac atcctgccgc acagcggtag gaaaactcag ctcgtcaatg gaactcagtg
     1441 tctccatcaa ggtgggtagg ctctcacgcg caatcacaag tacaaaatcc tcttggaact
     1501 ggttccgcgc tctgacatga gcggcctcca cgcagattat acccccgaca gacaaccgcc
     1561 cgtaggggta cacgcctgaa gccgcttcag gttccgaaaa gggaaagaga ttttttggtt
     1621 aagactttgg tgccggggag agctaggcgg agggattctt gaagagggcg tcgtccactt
     1681 tcactccgaa agtcacagtc aggatgcgcg tttcctgtgt ttgcttgccg tcctctaaca
     1741 gaatcgtgcg gaacgggacc agggtgcttt gcgcgtagcg ataatcagca aaacgtttcg
     1801 agaacttcgc ccctcgctct tcatactcta accaaagaat gtgaagcgtc ttggcgctga
     1861 cataaaagcg ggtcgttcgt tgttctttat caattaagtc gaggacataa aggtcgaggc
     1921 ccttttgttt gtccttacca atcagtttga cggtcgcgcc cgtttctttg tagcgcaaaa
     1981 gcgcttcgat gctgtgacgg tgctgcgaca caaacgccgc agctgcatcc tggcggggcg
     2041 tgaaagccgc gccgttgatc atgccccaga gctggccgcc accaaagatc agcgagtact
     2101 cgagcgtagg catcttttga tcaagacgaa tcttgtcgtt gtcgagggta tcgccgcgca
     2161 caaaaacgcg ttcgtacgta gcctcttcca tcttaccgtc gttcgccaat cgagttatcc
     2221 gtccccgctc aacaccgttt ctacgaatct gttgcagcac gggccgcgaa ccgtagataa
     2281 agatcgccga ctcagcaatc tgttcggcgg tgaaggtttt cgtgttcttg tcgaccttga
     2341 ccggcgcggg agtaggcgtc ggaacagagc tggttcggtc ttgagggttc agcgaactag
     2401 cgcccgaggt tggcgcgagt gcgatgattc caatcacgaa ggcacagaac accgcagggg
     2461 ctataaagag tcggaacaag aattcagttc tcccgttttc aggattgatt ttcgactaac
     2521 cggggaaagc aattcgtcag cggcggattc taccataagc aaaagccaaa gacgttgagc
     2581 gctctggccg tctccaagct cgcttgctgc atcgccattt gcagccagcg cagccaatag
     2641 gtaacgaaat gtggacgctg cggccgctta attgatgcca tttcatgaag aatcggatgc
     2701 ctgtggttcc caggtgtcat ttgacattgc ctcttttggc agcgtatttt ccaagctttc
     2761 caaccccgag aacatactta accgtgcgtc cggtgttatc ccaattgagc attccctgtg
     2821 cgcttcggtt gcgcattaat ttggctgaaa ggatattgag ttcatgaatc gagtcggtat
     2881 tttggtgggc cgtgaaaaga cctttcccga tgccctgatc aagacaatta acgagcgcgg
     2941 aaacggcaag accgtcgccg agttcataac cctgggcggt attaggcatg acgatccgcc
     3001 gcagtatgac gttgtagtcg atcggatttc acatgaagtc cctttctata gagcgacatt
     3061 aaaacggatg gctttggagg ggacggtcat catcaacaat cccttctggt ggtcagctga
     3121 cgataagttt ttcaactact ctttggctgc gaaactcggc gtggcagttc cccgcactgt
     3181 tctgctaccg cagaaggact acatcaaggg aatcgttagc gagagtctgc gcaatctcga
     3241 gtttcccctg gactggcaag gcatcgtgga gtatgtcggc ctgcccgcct ttctgaaacc
     3301 gttcgatggc ggtggctgga agaatgtctc aaagatcaat tccattgaag acctctggaa
     3361 ggaatacgat gagacgggca cgctttgtat gacccttcag gctgcagttg atttcgatca
     3421 atttgttcgt tgctattgtg tgggccagga ggaagtgatg atcatggcct atgatccgcg
     3481 caagccttac ctttccggcg aacagtacat tcaccatccg gattacctgg agcccgccct
     3541 gaaggatcgc gtcaccaaag atgtgcgcac tctctgcacg gccctcggct atgatctcaa
     3601 caccgtcgag tttgcgatca aggacggcgt tccctatgcg atcgacttta tgaatccggc
     3661 gccggatgcc gagctggctt cggttggtga gtactatcac aattggatcg tcggcgctgt
     3721 ggcagactta gtctttaaac gactggctga gccacggcag cgggcgcgtt atcgttggga
     3781 tgccttgctg aatccggaac cgcaatctac cgcagcggta gcctcacccg ctgagcagca
     3841 agcgcgtacg attgttccaa ctccagccac gacggaggca cctaaagcga aaacggcgac
     3901 agccgctacc gcgcggaaga aatccagccc cagtcgttca aagccaacgg cttaaactaa
     3961 aaaacccaaa cccgcgaagg cggactcggg tgcttataag ttaaggacag ggactcgttc
     4021 ctgtcctttt tct
//
LOCUS       DASUEX010000262         4366 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_854900, whole genome shotgun sequence.
ACCESSION   DASUEX010000262 DASUEX010000000
VERSION     DASUEX010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4366
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_854900"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(146..787)
                     /gene="lipB"
                     /locus_tag="VFV61_04425"
     CDS             complement(146..787)
                     /gene="lipB"
                     /locus_tag="VFV61_04425"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEX4899753.1"
                     /translation="MKKELAVRRLGRIDYEAALALQKEAERAVQSGEQPDTLLLLEHP
                     HTLTIGRRGDNSAILLPAASLAERGVTVFETNRGGKLTYHGLGQIVGYPIINLSPDRE
                     DVHRFVRDLEEVLIRTMADFEIAAFRIPGLTGVHTELGKVAAIGVHIARWVTTHGFAL
                     NVNTDLSYFDLIIACEGEPVTSMQKLLGREIELGEVEDRIVVHFGTVFDREPI"
     gene            1105..1614
                     /locus_tag="VFV61_04430"
     CDS             1105..1614
                     /locus_tag="VFV61_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899754.1"
                     /translation="MKRITFFLLVATILVPATTLAQTRGRKTTPARSTTTSLTDKRTI
                     GAGRVADQIKTLTRFIFVLGGVAKGLEGVDDAARRNEATPAVLEQAKQNKLTVRTSIR
                     KVREVLDQLESDFSSTIELRRYYTRLAGVAAGAAAAEEQAAANQFDKAGRSLLEVVNR
                     LTDVLLEMR"
     gene            complement(1681..2820)
                     /locus_tag="VFV61_04435"
     CDS             complement(1681..2820)
                     /locus_tag="VFV61_04435"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX4899755.1"
                     /translation="MNTKRWGKHLRPVLLWAALALFFCTSPVAGQRLKDLPAPPAPPR
                     LKPTPTPTPVPPPKDEDVDVIRVSSNLVMVPVSVVDANSQPVLGLQVTDFRVLELGRE
                     QQLTDLGNPDQVPLDIAILFDISSSVSQKGFFQFQQRAAASFLRQVMKPVDRAAIFTI
                     ADQPVLVAPLAPAESATLKLQQIPAATSPVPTAFYDTVAAAAKYLTEKSEERHRRVIL
                     VISDGDDNFSSLVRDMTKAEALAVQKGEETPASAKRSLDSRRARAVLEAQKAVQQADA
                     AFYSINPGGSSVHLNLISTRAQNAMQSIAESTGGNAFVPDGEQNLERVFNEIAAELRG
                     QYLLQYYSNSQTGAEFRSIKVSLPARPEIRVRARQGYYPKKTSSQ"
     gene            complement(2960..3976)
                     /locus_tag="VFV61_04440"
     CDS             complement(2960..3976)
                     /locus_tag="VFV61_04440"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX4899756.1"
                     /translation="MAIPKFKMFSLLMLVMGLMLVTAPVALSQGNTTVSSRTVASGEK
                     MKIKGVVTRRDADTFTVRDNNGVDTIVQLTDRTSVKAKGGFLRSGANYAQTQILRGLN
                     LEVEGRGSANGELVAEKVRFNESDLRVARAVESRAAPLEDRATTTETKLSQVEQNAQR
                     LSGQLDELAAVANTAKGGARAAQETADSAVAGVNATNDRISALDDYEPQTVAAVNFRS
                     GSAVLSKDSKATLDDVATKALNSKGYVLEVSGFADTTGSVARNRVLSQRRADTVIRYL
                     IENHNIPLRRIVTPYGYGESNPVAENNTRDGRAQNRRVEVKLLVNKGLLQSAPSMTTS
                     PTGN"
BASE COUNT          992 a         1210 c         1142 g         1022 t
ORIGIN      
        1 aagccgccaa gaaacgccaa ggcgccgcca agcatttttg aattacctta accgctctaa
       61 aaaacagggt ccccttgttg tctttccttt gcgtccttgg cgtttcttgg cggcttggcg
      121 gtaataaatt gtctcgcgca atactctaaa tcggctcccg gtcgaatact gttccaaagt
      181 gaaccacaat cctgtcttcc acctcgccta gctcaatctc gcgtcccagg agcttctgca
      241 ttgaggtcac cggttctcct tcgcaggcaa tgatcaagtc gaagtagcta agatctgtgt
      301 ttacattcaa ggcgaacccg tgcgtcgtta cccagcgcgc aatgtgcacg ccaatagccg
      361 ctacctttcc caattcagtg tgaacacccg ttagccccgg aatgcgaaaa gccgcaatct
      421 caaaatcggc cattgtgcga atcaaaactt cttcaagatc ccggacgaag cgatgcacat
      481 cttcgcggtc cggactgagg ttaatgattg ggtaaccaac aatctggccc aggccgtggt
      541 aggtaagttt accgccgcga ttggtctcaa agaccgtaac gccgcgctct gccaatgaag
      601 cagcgggcaa gaggattgcc gaattatctc cgcgccggcc gatggtgagc gtgtgcggat
      661 gctcgagcaa tagcagcgta tccggttgct cacccgattg caccgcgcgc tcagcttctt
      721 tttgtagcgc cagcgcggct tcgtaatcga tacgtcccag gcgccgcacc gcgagttctt
      781 ttttcatcac catcatagct acggtacaac catcttactg tcgaatgcgt cattctatca
      841 tctagggcag taggcagtag gcagtaggca gtaggcagta ggcagtaggc agtggtcggt
      901 ggttgcgtta actacaaata agagtgaatt gtgcagcacc tcgtacgctg ccagcctact
      961 ttaattacaa ccggccactg actacaaaac aactgaccac tctaaacttt attggcctgc
     1021 ttgactgtca cgagtgcgag gcataccttt ggacgcacac taaaccaaac tcaatcgcga
     1081 atgaatcatg agggagagca caccttgaaa cgaatcactt tttttctgct cgttgctacc
     1141 attctggttc cggcgactac gctggcacaa acacgcggtc gtaagactac gcccgcacga
     1201 tctaccacga catcgcttac cgataagcga accatcggag ctggacgcgt tgccgatcaa
     1261 ataaaaacgc tgacccgctt tatctttgtg ctcggcggcg tggcgaaggg cttagaaggc
     1321 gttgatgatg ccgctcgtcg caacgaggca actcccgccg tcctggaaca agcaaagcag
     1381 aacaagttaa ctgtgcgcac gagcattcgg aaggtgcgtg aagttcttga tcagctcgag
     1441 tctgatttca gctcgaccat cgagttgcgc cgctattaca cgagactggc gggagtagcc
     1501 gcgggcgccg ccgcggccga agaacaagct gcggcaaatc agtttgataa agccgggaga
     1561 tcgctgcttg aagtcgtcaa ccgacttacg gatgtgttgt tggagatgcg ttaagaagtt
     1621 ccatgtttca ggttccaagt ttgaacctga aacctgaaac ttaaaacatg acacgaggaa
     1681 ctattgactt gaggtttttt tagggtagta gccttgccga gcgcgtacgc gtatttccgg
     1741 gcgcgcgggg agcgaaactt tgatactgcg aaactcagca ccggtctgcg aattcgaata
     1801 atactgcaag agatactgtc ctcttagttc tgccgcaatc tcattaaata cccgctccag
     1861 attctgttcg ccgtcaggca caaaagcatt cccaccagta gactcggcaa ttgactgcat
     1921 cgcattttgc gcgcgcgtac tgatcagatt gaggtgcacc gaggacccgc cgggattgat
     1981 cgaatagaat gccgcgtcag cttgctgaac agccttttgg gcttccagca ctgcgcgagc
     2041 gcgacgcgag tcgagactgc gcttggcgct ggctggcgtt tcttcgccct tctgcacggc
     2101 gagcgcttca gcctttgtca tatctctaac gaggctgctg aaattatcgt ctccatccga
     2161 gatcaccaga attacccgcc ggtgacgctc ttccgatttc tcagttaagt attttgccgc
     2221 tgcggcaacg gtgtcgtaga acgcggtggg aaccggcgac gtggctgcag gaatctgttg
     2281 cagctttagg gttgccgact cggccggcgc caaaggtgca accaacaccg gctgatcggc
     2341 aatggtaaaa attgcagccc ggtcgacggg cttcatcacc tgtcttagaa aactcgcagc
     2401 cgctctttgt tggaattgga aaaagccctt ctggctgacg ctgctggaaa tgtcaaaaag
     2461 gattgcgatg tcgaggggca cttgatccgg gtttcccagg tcagttagtt gttgctcgcg
     2521 tcccagttcc agcacgcgaa agtcagtgac ttgcaggccc agcaccggtt ggctgttcgc
     2581 atccacaacg gaaacaggaa ccattaccag gttagagctc acgcgaatga cgtcgacgtc
     2641 ctcgtctttt ggtggcggaa ccggtgttgg tgtaggcgtg ggtttaagtc tgggaggcgc
     2701 cggcggcgcg ggtagatctt tcagtcgctg ccctgccacc gggctggtgc agaaaaaaag
     2761 agccagcgct gcccaaagca gcactggcct caaatgtttt ccccaacgtt tagtgttcat
     2821 cctgccaatc ctcatgccgc cgtctgctgg ccaagcatgt tacttcaatt cgtcgtgtcg
     2881 agcgacggca tgattcatgg caactaagac tagcagtggg agacttatag ttgcacttcc
     2941 cgctgctact cgattcacac tagtttccag ttgggctggt cgtcattgac ggcgctgact
     3001 ggagcaggcc tttgttgacc agcaatttaa cttcaacgcg gcgattctgc gcgcggccat
     3061 cacgagtatt attctcggcc accggattcg attcgccata gccataaggc gtcacgatgc
     3121 ggcggagcgg aatattgtgg ttctcgatca ggtaacgaat cacagtatca gcacgacgtt
     3181 ggctcagcac acgattgcgc gcaacgctgc ccgtggtatc tgcgaagcct gaaacctcaa
     3241 gcacatagcc cttggagttc agtgctttgg tagcaacgtc gtcgagcgtc gctttcgaat
     3301 ctttcgagag caccgcactg ccggaacgga aattcacggc tgcaaccgtc tgcggttcat
     3361 agtcgtccaa agccgaaatg cgatcgttgg tggcattaac gccagcgaca gcagagtcgg
     3421 ctgtctcttg agcggcacga gcaccaccct tggcagtgtt ggcgaccgcc gccagctcat
     3481 caagctgccc ggagagacgc tgtgcattct gctcaacctg gctaagtttt gtttcagtag
     3541 tagtggcacg atcctcgagc ggcgcagcgc gtgattccac ggcacgggcg actcttagat
     3601 cggattcatt gaaccggacc ttctcggcga ctagctcacc gttagcactg ccgcggccct
     3661 cgacctcgag gttcagcccg cgcaggattt gcgtttgggc atagtttgcg ccactgcgta
     3721 ggaaaccgcc cttcgctttc acactggttc tgtcggtcaa ctgcacaatg gtgtcgacac
     3781 cgttgttatc acgaacggtg aacgtatcag cgtcgcggcg cgtgaccacg cccttgatct
     3841 tcatcttctc gccactcgcg actgtgcgcg agctgaccgt cgtgttgccc tgcgagagag
     3901 ccaccggtgc tgtcacaagc atcaggccca taaccaacat cagcaaggaa aacatcttga
     3961 acttcgggat tgccatagaa acctatctcc ttatcttcga aagtgggtga cccacacaac
     4021 tgcgagccgg gaatcgtcaa actctgacga taaattcttt cagtttgttg tccgcattga
     4081 acgctatgcc cacctgttct agtcgctagc taatgttaag tcaagacgtt tgccattact
     4141 agcttcattc ttcatcgaat cgtcacttga tcgcaataag tacgggcttc attacaaaat
     4201 gcggtgacaa aaccagcaaa agggtttgcc accgcgagct tgcaaaacag acttgtatcc
     4261 ggctttagat agagaattct ccggcttcta gttcatcttt tacgaacgcc agaaagcgtt
     4321 cggcatcggc gccgtcaatc actcgatgat caaagctgag cgcaaa
//
LOCUS       DASUEX010000263         2348 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1084831, whole genome shotgun sequence.
ACCESSION   DASUEX010000263 DASUEX010000000
VERSION     DASUEX010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2348
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1084831"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1557)
                     /locus_tag="VFV61_04445"
     CDS             complement(<1..1557)
                     /locus_tag="VFV61_04445"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX4899757.1"
                     /translation="MKRCPQCNRVENDGSLGFCRVDGTALISDSGPVNPEAGTVRFAS
                     APLPNEAETSVLPHPSTSPAVNRLTPPTTVLPAQRPPGATQELGKPKRRKAVLAITSL
                     IAVALIAGVYLYWPRGKRATAPIESIAVMPFANESGNADLEYLSDGMTETLINSLSQI
                     PNLSVKARSSVFRYKGKEIDPKEVASELGVETILTGRVVQRGDQLTLSVELIDGQTEN
                     TIWGNKYERKGSDLVALQSEVARDLSTKLKTKLSGADVAKVEKTYTTNPDAYQLYLKG
                     KFNWNKRTGESLQQAAELYRQAIEKDPNYALAYSGLAETYVLFSSYDVAPATDSMPHA
                     KAAALRALEIDDSLAEAHTALGFYLSNYEWDRVGSEKEYRRAIELKPNYATAHHWLGA
                     DLGNLKRFDESLVELKRAEELDPLSLIIGTNLGDTLVFARRYDEAIAQYKRTLVRNPK
                     FVYAHQALSRAYGATGRYAEAIAETRMAIDLNSGSSAKGYLGLWLAKSGKRDEAVKLL
                     GELKQESTRNY"
     gene            complement(1734..>2348)
                     /locus_tag="VFV61_04450"
     CDS             complement(1734..>2348)
                     /locus_tag="VFV61_04450"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX4899758.1"
                     /translation="NYPTAHQWYAVYLMSAGRFDEALAQNRRAQELDPLSIPINMTLG
                     WCLLNARQYDQSVDQLSKTLEMDPNFVLAHHRLGMVYELQGKYDKAIAEFRQVVNLTG
                     EKPLGIAGLARAYALSGRRAEAQKTLAELLEASKQRYVAPTLIAMVYAAFGDKDQAFA
                     WLEKAEKVRDALLARLKVDPRFDSLRSDPRFKDLVRRVGLPESK"
BASE COUNT          516 a          653 c          611 g          568 t
ORIGIN      
        1 ataattccgg gttgattcct gttttaactc gccgagcagc ttcactgctt catcgcgctt
       61 gccggatttg gccagccaca gtcccaaata tccctttgct gaggaaccac tattcaggtc
      121 gattgccatg cgcgtttccg cgatggcttc cgcatacctt cccgttgcac catatgcccg
      181 actcaaagcc tggtgagcgt aaacaaactt tgggttacga acgagagtac gtttgtattg
      241 cgcgatggcc tcatcatacc gccgagcgaa gactagggtg tcaccgagat tggtaccaat
      301 gatcagggaa agcgggtcaa gttcctcggc acgctttaac tcgaccagac tctcgtcaaa
      361 ccgttttaga ttcccgagat cagcacctaa ccaatgatgg gcggtcgcgt aattcggttt
      421 gagttcgata gcgcgacgat actccttttc cgagcccacc cgatcccatt cataattgga
      481 aagataaaat ccgagcgcgg tgtgtgcttc tgccagcgaa tcatcaatct ccaacgcccg
      541 cagagccgcc gctttcgcgt gaggcatgct atcagttgcc ggagcaacat cgtagctgga
      601 aaagagaacg tacgtttcag ccaaacctga ataggcgagg gcatagttcg ggtctttctc
      661 gatcgcctgg cgataaagct cagcagcttg ttggagggat tcgcccgttc gtttgttcca
      721 attgaatttt ccctttagat agagctgata agcgtcggga ttagtcgtgt aagtcttttc
      781 gactttcgcc acgtccgctc ctgaaagctt cgttttaagc ttggtcgaaa ggtcacgggc
      841 gacctcgctt tgcagtgcca ccagatcaga gccttttctc tcgtacttat tgccccagat
      901 cgtgttctcc gtctgcccgt cgatcaattc cacgctcaag gtcaactgat caccccgttg
      961 gacgacgcgg ccggttaaga tcgtttcgac acctaactcc gaagctacct ctttcggatc
     1021 gatctcctta cctttgtacc taaagaccga agagcgagcc ttgacgctca agtttgggat
     1081 ctgcgaaagg ctattgatga gcgtttcggt cattccgtcc gagagatatt cgaggtccgc
     1141 attgccgctt tcgttggcga atggcatcac cgcgatggat tcaataggtg cggtggctct
     1201 cttacctcgg ggccagtaga ggtagacgcc ggcaatcaga gcgacagcga tgagtgaggt
     1261 tattgccaag accgcttttc gccgtttcgg tttgcctagc tcttgtgtcg cgcctggtgg
     1321 ccgctgcgca ggaagcacag ttgttggtgg agtcagacga ttgaccgctg gagacgttga
     1381 tgggtgcggc agaacgctgg tctcggcctc gttcggcagc ggagcagagg cgaacctcac
     1441 tgtaccggcc tcagggttaa cgggaccaga gtcactgatc aaagccgtcc cgtccacgcg
     1501 acagaaccca agcgagccat cgttctcgac acgattacat tgggggcagc gcttcataaa
     1561 ttgcagagca agcggttagc ttgccactcc ggcgccgaac gcaaaccaac agtttatagc
     1621 ccagatcaag acaaatgaat ctcgacgaag gcgccggcgc ctcggctcca aatctataag
     1681 gattccgccg cttccggagc gaatggctga actgagtttt ccagtccggc aatctacttc
     1741 gattctggca accctacacg tcgaactaga tctttgaagc gcgggtccga ccgcaaactg
     1801 tcgaatctgg gatccacttt gaggcgtgcc aacaaggcgt cgcgcacctt ttctgccttc
     1861 tccagccaag cgaatgcttg atctttatca ccgaaggcgg cgtacaccat tgcgatcaaa
     1921 gtgggcgcaa cgtaacgctg ctttgatgct tccagcaact cggcaagagt tttctgcgcc
     1981 tccgcgcgtc gtccagagag tgcatacgca cgcgccagcc ccgcgatccc taagggtttc
     2041 tctccagtca gattgacgac ttgtctgaac tcagcaatgg ccttgtcata ctttccttgt
     2101 agttcgtaga ccataccaag ccggtgatgt gcaagcacaa agttcggatc catttccagg
     2161 gtcttactca attggtcgac gctttgatcg tattgacgag cgttaaggag acaccatcct
     2221 agagtcatgt tgatagggat cgaaagcgga tccagctctt gtgctcgtct attctgcgca
     2281 agggcctcgt cgaaccggcc cgccgacatt aagtaaacgg cataccactg gtgagccgta
     2341 ggataatt
//
LOCUS       DASUEX010000264         2008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1291641, whole genome shotgun sequence.
ACCESSION   DASUEX010000264 DASUEX010000000
VERSION     DASUEX010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1291641"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="VFV61_04455"
     CDS             complement(<1..311)
                     /locus_tag="VFV61_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899759.1"
                     /translation="MRKKLLFVVWLIVLTALAGFAQSARRPLKLDDLARFRNVGDPQI
                     SPDGQAIAYTVSTIDAKEDKSNTHIWLVAYDGTNDRQITFSNDSESSPRWSPDGKYLA
                     FT"
     gene            complement(508..1719)
                     /locus_tag="VFV61_04460"
     CDS             complement(508..1719)
                     /locus_tag="VFV61_04460"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX4899760.1"
                     /translation="MAKPRLNLTCVANALLLLAVTLTSPVFAQTGDLAQTKQKAVALI
                     DDLKYVEAFPLLEKIVAAEPDNSEMQFYLGFALVAKANETTDASMRRDFRVRARAAFV
                     RAKQLGYGAPLVDALITSMPPDGSEGAAFSSNAAAEAIMKTAEAHFSKGELDDALKNY
                     QRALVLDPKLYHAALFSGDVYLHQNHYEQAEFWYQRAISIDPNRETAYRYSATPLMRQ
                     RKFDQARDRYVEAYITEPYSRFAISGISQWAQATNTPLGHPQIDIPVTVTFKDNGDAN
                     ISLDPAALGQEDGSFAWIGYGLTRTTWRKDKFAKTYPKETTYRHSLAEEADALRSVVM
                     VAASDKRTKTLTPSLAKLKKLNDEGLLEAYLLLARADQGIAQDHRAYLATNRALLRRY
                     VMEYVVRGGGN"
     gene            complement(1751..>2008)
                     /locus_tag="VFV61_04465"
     CDS             complement(1751..>2008)
                     /locus_tag="VFV61_04465"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX4899761.1"
                     /translation="KGETPNKKQLKASLDISAKAVETTIRESWANGGKTPGFKRGLLP
                     LLGYLIAHESHHRGSILLTLKQTGHKLTDELRWGLWDWAHI"
BASE COUNT          404 a          584 c          546 g          474 t
ORIGIN      
        1 gtgaacgcga gatattttcc gtcaggactc cagcgcgggg aactttcgct gtcgttgctg
       61 aaagtaatct gccgatcgtt cgtgccgtcg taagcaacca gccagatgtg cgtgttgctc
      121 ttgtcttctt tcgcgtcaat cgtcgagacg gtgtacgcga tcgcttggcc gtcaggcgaa
      181 atctgcggat cgcccacgtt gcggaaacgc gcgagatcat cgagcttcag tggccggcgc
      241 gcgctttggg caaagcctgc cagggcagta agaacgatta gccaaacaac aaagagaagt
      301 ttctttcgca cgattcaact acctccggtt ttaatgtccg tccgactaac cacagagggc
      361 acagagaagg cctgcccgca aagagcaaag cggcaagggc taagggcaaa gcgtcagccg
      421 tacagtttac gcgctagctc tgtgccctta cctctttgcg cttgccttgt cttcctctgt
      481 gtcctctgta gttaatttgt ggatgcgcta attaccgccg ccacgcacca cgtactccat
      541 cacataccgc cgcaagaggg cccgattcgt cgcaagatac gcgcgatgat cctgggcgat
      601 gccctggtcc gcgcgtgcca gcagcaagta agcctcgagc agcccctcgt cattcaactt
      661 cttcagtttc gccagcgaag gggtgagcgt ctttgtgcgc ttgtcgctgg ccgcaaccat
      721 caccacgcta cgcaacgcat cggcttcctc tgccaggctg tgccggtaag tcgtctcttt
      781 cggataagtt ttggcgaatt tatccttgcg ccacgtagtc cgagttaagc cataaccaat
      841 ccaggcgaac gatccatcct cctgcccgag cgccgccgga tccaaactga tgttcgcatc
      901 accgttgtct ttgaacgtca ccgttaccgg gatgtcgatc tgcggatggc ccagcggggt
      961 gttcgtcgcc tgcgcccact gcgagatccc ggaaatcgcg aaacggctgt aaggctcagt
     1021 gatgtacgct tcgacgtaac ggtcgcgggc ttgatcgaat ttacgctgcc tcattaacgg
     1081 cgtcgccgaa tatcggtagg cagtttcgcg attgggatcg atcgaaatcg ctctctgata
     1141 ccaaaactcg gcttgttcgt agtggttctg gtgcaagtac acatcgccag agaataacgc
     1201 cgcatgataa agcttcggat caagcaccag cgccctttga taatttttca gcgcgtcgtc
     1261 gagttcacct ttcgaaaaat gcgcttcggc ggtcttcatg atcgcttccg cggccgcgtt
     1321 ggatgaaaac gcagcgcctt cgctgccatc cggtggcatg ctcgtaatca acgcatcgac
     1381 caaaggcgcg ccgtatccca actgtttggc gcgcacaaat gcagcccggg cgcggacgcg
     1441 gaaatcccgg cgcatgctcg catcagtcgt ttcgttggct ttcgcaacca aagcgaagcc
     1501 caggtagaac tgcatttcgg aattgtcggg ctcggcggcc acgatctttt ccaacagtgg
     1561 aaaggcttcg acatacttca gatcgtcaat cagcgccacg gctttttgtt ttgtttgtgc
     1621 gaggtcaccg gtttgcgcga atactggaga cgtcagcgtc accgcgagca acagcaacgc
     1681 gttcgcgacg catgtgagat tcagtctcgg tttagccatt gagagagctc ctttggcatc
     1741 gatgttaaga ctagatgtgc gcccagtccc acaagcccca acgcaattcg tctgtcagct
     1801 tgtgtccggt ttgtttcagc gtcagcagga tgctaccgcg gtggtgcgac tcgtgcgcaa
     1861 tcaggtaacc aagcagcggc agcagaccgc gtttgaatcc cggcgtcttg ccgccgttcg
     1921 cccacgattc gcgaatggtt gtttccacag ctttcgcgga aatgtcgagg ctcgccttca
     1981 gttgcttctt gttgggcgtt tctccttt
//
LOCUS       DASUEX010000265         2561 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_855836, whole genome shotgun sequence.
ACCESSION   DASUEX010000265 DASUEX010000000
VERSION     DASUEX010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_855836"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /locus_tag="VFV61_04470"
     CDS             <1..732
                     /locus_tag="VFV61_04470"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX4899762.1"
                     /translation="LEVETLAGKSFLDIGSGSGLFSLAARRLGARVHSFDFDPQSVAC
                     TAELRRRYFPNDPQWKVEEGSALNADYVTSLGLFDVVYSWGVLHHTGEMWQGLANAVA
                     PVAPNGKLFIAIYNDTGTQTKRWKWIKRTYNGLPQFLKSPFALLAIAPDEMKAALRAL
                     VTMRPGQYIRSWTQYDRNNRGMSRWHDVIDWVGGYPYEVATPDEIFDFYRKRGFTLLQ
                     MRCGKVGLGCNEFVFRKADDYRENN"
     gene            729..1940
                     /locus_tag="VFV61_04475"
     CDS             729..1940
                     /locus_tag="VFV61_04475"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX4899763.1"
                     /translation="MRKNVLQLIGSFHQGGSERQAVQLTRLLRESGRYNVFVATLNNS
                     GPLAGEIANMGIDEVPEYRLTSFQDRNALVQGRRFAQYLRENDIDIVHSHDFYTNIFG
                     MAAASVARVRVRIASRRESAVRAVKQRLIERGAYKLAHQVVANCDELRQQLIEKEGLR
                     PDKVTTLYNGLDLSRIVARDVNSEKELLTSLGLPADQNRRFITIVANLRAHFTQPKPI
                     CLKDHPTFLRAAQRVRAAVPDAAFVIAGEGELLEETRALARQLGLEQDVFFLGSCQRV
                     AELLSASTICVLSSTAEGFPNAILEYMAAARAVVSTDVGGAREAIEEGQSGYLVKSGD
                     DETMAARLVSLLNEPDKARAMGDRGRGIVKEKFSSEAQLARTQNLYERLLARRTQSVP
                     GTVGDVHRGNV"
     gene            2084..>2561
                     /locus_tag="VFV61_04480"
     CDS             2084..>2561
                     /locus_tag="VFV61_04480"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX4899764.1"
                     /translation="MEGLAEEKSVRRGFLPHNPRMPPGFRWLTRIKYVRTAVTTLLYW
                     TLLLARAWRYDILHIFSASYYSYALSVAPAILVAKLYGKKCILNYRSGEAEDHLKNWR
                     ITAIPTMRLADVIVVPSGYLIEVFARFGLKARAIHNIVELERFQYRERRPLRPVFLT"
BASE COUNT          567 a          645 c          740 g          609 t
ORIGIN      
        1 ctggaagtcg aaacactcgc cgggaagagt tttctggata tcggttctgg cagtggactc
       61 tttagcctgg cagcgcgccg tcttggtgct cgagttcact cctttgattt tgatccacag
      121 tcggtggcct gtacggctga acttcgtcgt agatactttc ccaacgatcc gcagtggaag
      181 gttgaggaag gttcagctct aaacgctgac tatgtcacat cgctggggtt gtttgatgtc
      241 gtctactcct ggggagtact tcatcacacc ggagagatgt ggcagggcct ggcgaatgcc
      301 gtggcgcccg tggctcccaa cggtaagttg ttcattgcga tctataacga taccggaaca
      361 cagaccaaac gttggaaatg gatcaaacga acctacaacg ggctgccgca attcctgaag
      421 tctccattcg cgttgcttgc tattgccccc gatgagatga aggcagcgct ccgcgcctta
      481 gtgaccatga gacccggtca gtacattcga tcctggaccc agtatgatcg gaacaatcgt
      541 gggatgagcc gctggcatga cgtcattgat tgggtcggcg gatatccata cgaagtcgct
      601 actccagacg aaattttcga tttctatcgc aagagagggt tcacattgct gcagatgaga
      661 tgtgggaagg tgggcctcgg gtgtaacgaa ttcgtgtttc gaaaagcaga cgattatcgg
      721 gagaacaatt gaggaaaaac gttctccagc ttatcggcag ttttcaccag ggtggctcag
      781 aacgccaggc cgtgcagttg actcgtttgt tacgcgagag tggccgctac aacgtgttcg
      841 tagcgacgct caataacagt gggccactgg ctggtgaaat cgcgaacatg ggaatcgatg
      901 aagtacctga gtatcgcttg actagtttcc aggatcgcaa tgccctggtg cagggccgcc
      961 gttttgcgca gtatctcaga gagaatgata tcgacatcgt gcacagtcac gatttttata
     1021 ccaacatctt tgggatggcg gctgcttccg tggcgcgtgt gagagtacgc attgcctcac
     1081 gtcgcgagtc ggctgtgaga gcagtaaagc agcggttaat tgagcgcggg gcttacaaac
     1141 ttgcgcatca agtggtggct aattgtgacg agcttcgcca acagctaata gagaaagagg
     1201 gtctacggcc agacaaagtc acgactttgt acaacggact cgacctcagc aggattgtcg
     1261 ctcgcgatgt aaactcagaa aaagagttac tcacctcgct tggtttaccg gctgaccaaa
     1321 accgccggtt tatcaccatc gtcgccaatc ttcgtgcgca ctttacccaa cccaaaccta
     1381 tttgtcttaa agatcaccca acgttcctga gggcagccca gcgcgtgcgc gccgcggtgc
     1441 ccgatgcggc tttcgtgatt gccggcgagg gcgaactgct ggaagagact cgcgcgctgg
     1501 cccgtcaact cgggctcgaa caggacgtct ttttccttgg ctcttgccag cgagtggcag
     1561 agttgctttc ggcgtccacg atctgtgtct tgagttcgac cgccgaaggc tttccaaacg
     1621 caatcctcga atacatggcc gccgcacgtg cggtggtttc caccgatgtt ggcggtgcgc
     1681 gggaagccat cgaggaaggc caatccggct acctggtcaa gtctggggat gacgaaacaa
     1741 tggcagcccg gttggtgtcg ctcttaaatg agcccgacaa ggcgcgtgcg atgggggacc
     1801 gaggtcgtgg aattgttaaa gagaagtttt ccagtgaagc ccagttggcc aggacgcaga
     1861 acttgtatga gcgcctactt gctcggcgta ctcagtccgt gccagggaca gtgggtgatg
     1921 tgcaccgcgg gaatgtgtag ttcggaggcc atcgaggttg gatacgctca atccaggaag
     1981 gaatgaacaa gaacagcgcg gagtgacatc tcctctgcgc gtcctctttc ttgctccgtc
     2041 tctggacatt ctaggcggcc aatcacggca gtgtgcgcgt ttgatggagg gactggctga
     2101 agagaagtcg gttcgaagag gttttctgcc tcacaatccg agaatgccgc cgggtttcag
     2161 atggctcact cgcatcaagt atgtccgaac cgcagttaca acacttctct attggacgtt
     2221 gttgcttgcg cgagcgtggc gttatgacat cctgcacatc ttttcagcct cgtactattc
     2281 gtatgcattg tccgtcgcgc cggcgattct ggtggcgaaa ctttatggga agaaatgcat
     2341 tcttaattat cgcagcggtg aggctgagga ccatctgaag aattggcgca ttacggcgat
     2401 accgaccatg cgcctggcgg atgtaattgt ggtcccctcc ggttacctga ttgaagtgtt
     2461 tgcgcggttc ggattaaagg cgagagcaat tcataacata gttgagctgg aacgatttca
     2521 atatcgtgaa cgacgcccgt tgcgacccgt ctttctaacc a
//
LOCUS       DASUEX010000266         3675 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1637510, whole genome shotgun sequence.
ACCESSION   DASUEX010000266 DASUEX010000000
VERSION     DASUEX010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3675
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1637510"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            124..858
                     /locus_tag="VFV61_04485"
     CDS             124..858
                     /locus_tag="VFV61_04485"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEX4899765.1"
                     /translation="MSLTLLGIFIGTLVFEAFMFVRVARLRTHNPGSTSLMETRDNEA
                     MARGQQPRREQIWVSLNQISPNLQRAILAGEDTNFLTHHGFDYEAIQKVWDQAQREAA
                     KEAKAEGDEEGDWLPSLPDFKRGASTVTQQLAKNLYLSSQKNFIRKGQEAVLTVFLER
                     LLTKRRILEIYLNVIEWGDGVYGAEAAARRYFNKSAASLTANEAAYLSAMVPNPRTVF
                     NPRINPRRVARRQRIIMRGMPYQKIP"
     gene            complement(1321..1683)
                     /locus_tag="VFV61_04490"
     CDS             complement(1321..1683)
                     /locus_tag="VFV61_04490"
                     /inference="COORDINATES: protein motif:HMM:NF015232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HEX4899766.1"
                     /translation="MFKELLETIIEHTEGSLGALIMGTDGIAVEKVLGEANDLNLDIA
                     AAEFTSLVRSSQRAGTDTGLGGLHELVISLDSAILIMRLLSRDYFVVLAMSPEGNLGR
                     GRFELRKAELQLAKEFAV"
     gene            complement(1696..>3675)
                     /gene="pilQ"
                     /locus_tag="VFV61_04495"
     CDS             complement(1696..>3675)
                     /gene="pilQ"
                     /locus_tag="VFV61_04495"
                     /inference="COORDINATES: protein motif:HMM:TIGR02515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HEX4899767.1"
                     /translation="QMSVARGGHVQNSLKEGTMPYQSPILAAIKRTALVLLCLSLAGA
                     NTLAGTTASRPATSKGATLKLVNYIPLSAQAEARLAAEIVAAHDAENQDPAVAQNQSQ
                     TKPGISTGTASQSDQGRQYGQPGFVGEPINLNVVNADIRDILNYITEQYGVNFVIDSS
                     VRSVPVTVNVQDVPWNVALEAILRANRLGIETSDNILRVATTDVLAKEAETQKVINDS
                     RLDASPLVTEFIRLNYARASGTLAQAAGSTGAFSGGATNLSFSGGAGGDATGGSGDQG
                     LLPIVSRRLSRRGSIEVDGRSNTLIITDVKVNIESIRQLVTILDQPEPQVEIETRIVI
                     AQRNFSRDLGAQISAGVLNTNRGGLGNISTLPSTPPVSGQVKQGVFGGLVGTPQPIPS
                     LAAAAASTVIGLTTGLFGTAQISALITAAELRGTAKTVATPKVTAINNRPAQIESGSQ
                     IPVQTTQAAAGTAVVTTTFVSVPLRLSITPQITDAGTVILRVTIENNTINTGIAVGGV
                     PGIDTQRMQSEVLVPDGGTTVMGGVLADTEGETQQRTPGLASIPVLGNLFKRKLTTSS
                     SREILFFITPRIYRPDYQGKPIKMEPINAPRTTTISQPVPMGNPQSNTPTPTQLQQQQ
                     NAPTQQPNQLLNQAPTLPDASAPSARPGSLGGSRP"
BASE COUNT          808 a          982 c         1068 g          817 t
ORIGIN      
        1 tataagatcg gtagctgagg tttttagaag gctcggtcgg gcaagctctg cgctgcaatc
       61 tgcgaatgga tgaggaaaac aggtcacaga aaaagaggcc gtatccgcaa gttcctccga
      121 gtagtgtctc tgacgttgct cggaatattt atcggcacac ttgtttttga agcattcatg
      181 tttgtgcgcg tggcgcgttt acgaactcat aatcctggct caacgtcact aatggaaacg
      241 cgcgataacg aggccatggc ccggggccaa caacctcgcc gcgaacaaat ctgggtgtct
      301 ctaaatcaaa tctctcccaa cctacagcgg gcaattctcg ccggcgaaga cacaaacttc
      361 cttactcatc acggctttga ttacgaagcg atccagaagg tttgggatca ggcgcagcga
      421 gaagcggcga aggaagcgaa ggctgaaggc gatgaggagg gcgattggct cccgtcactt
      481 cctgacttca aacgtggcgc ttcgacagta acgcagcagt tggcgaagaa cctctattta
      541 tcttcgcaaa aaaacttcat ccgtaaaggg caggaggccg tgctcacagt tttcctggag
      601 cgtttactaa cgaagcgacg cattctggaa atctatctca atgtgatcga atggggtgat
      661 ggagtttacg gcgccgaggc cgcggcccgc cgctatttta ataagtcggc tgcgagtctg
      721 acggcaaatg aggcagccta tctttccgcg atggttccca atccccgcac ggtttttaat
      781 ccgcgcatca acccgcggcg cgtcgcgcgc cggcaacgga tcatcatgcg cggcatgccg
      841 taccagaaga tcccttagga aacaggaggg gtgtaatcga agaccgatcg tctccattgc
      901 cgattgtcga tatccaattg cggcgccaga ttaaaggcta atgacttgac cagaggctaa
      961 tgcaatcaag gttgaaatcg gggattcaag tacgaaaagc aacgtccgaa gcgccaacgg
     1021 cgcgaactga aatagccagg ggcaagtgct gagcgcagcg aagcacgtcg cccctggaca
     1081 gtagaagaca ccatttgctt ccctttccag gggcggcgcg ccttcgctgc gctcagcgct
     1141 tgcccctggc tatcacattc ccgcgccttt ggcgctcaag attagacgat tccacacttc
     1201 aactcgttga acttttgaat tgaattcgcc attggcagtt ggcaatcaaa aatcggcaat
     1261 caacgaagaa gccgcccgtg ggaagggcgg cttcgcaaaa acgttagtgc ggcgacggtg
     1321 ttagacggca aactccttcg cgagttgtaa ctcggcctta cgcaattcaa aacgcccgcg
     1381 accaaggtta ccctctggcg acattgccag caccacaaaa taatcgcgac tgagcagtcg
     1441 cataatcaga atcgcggagt cgagtgaaat caccagctcg tggagaccac ccaaacccgt
     1501 atcagtgccg gcgcgctgtg aactgcggac cagcgaagtg aactcggcgg cggcaatatc
     1561 gaggttcaga tcattggcct caccgagcac cttctctact gctattccat cagtgcccat
     1621 gatgagcgca ccgaggctgc cctcggtgtg ctcaataatg gtttctaaca gttctttgaa
     1681 cattgcttgt cttccttaag gacgcgagcc gcctaaagat cccggccgcg ctgagggagc
     1741 cgaagcatcc gggagcgtgg gtgcctggtt caataattga tttggctgct gcgtgggagc
     1801 gttctgctgt tgttgcagct gcgtcggggt aggtgtgttg gattgtggat tacccatcgg
     1861 caccggttgc gaaattgtcg tggtacgcgg ggcattgatg ggttccatct tgatcggttt
     1921 gccctggtaa tctggacgat agatccgcgg agtaatgaaa aagaggatct cgcgactcga
     1981 gctggtcgtc agctttctct taaataggtt tccgagtacg ggaatcgaag caaggcctgg
     2041 cgtgcgctgc tgagtctcgc cttcggtgtc tgccagcaca ccgcccataa ctgtcgtgcc
     2101 tccatctgga accaggactt cggattgcat gcgctgcgta tcgatgcccg gtacgccgcc
     2161 gacggcgata ccggtattga tggtgttgtt ttcgatcgtg acgcgaagga taacggtacc
     2221 ggcgtcagtg atctgcggcg tgatcgagag acgcaagggc acggagacga aggtggtcgt
     2281 tacaacagca gttccagcgg cggcttgagt agtctgcacc ggaatctgcg atccactttc
     2341 aatctgcgcg ggacgattgt taatggccgt gacttttgga gtggcgacag tcttcgccgt
     2401 tccgcgcaac tctgcggctg taatcaaggc gctgatctga gcagtgccaa acagcccggt
     2461 ggtcagaccg atgacggtgc tggctgctgc cgctgccagc gatggaattg gctgcggcgt
     2521 tccgacgaga ccgccgaaga cgccttgctt aacctgaccg gagaccggcg gagtggaagg
     2581 cagggtcgaa atgttcccca gtccaccgcg attcgtattc aacacaccag cagagatttg
     2641 ggcgccgaga tcgcgactga agttgcgctg ggcgattacg atccttgttt caatttcgac
     2701 ctgaggttcc ggttggtcga ggatggtgac gagctggcga atagattcaa tgttcacctt
     2761 cacgtcggta atgatcagtg tgttgctgcg gccgtcgact tcaatcgagc cacggcgaga
     2821 tagccggcgc gaaacgatcg gcagtaatcc ctgatcacca ctgccaccgg tagcgtcgcc
     2881 accagcgccg ccactgaagc taaggttggt ggccccgcct gagaacgcac ccgtggagcc
     2941 tgcggcctgc gccagagtgc cggaagcgcg cgcgtagttt aagcgtatga attcggtaac
     3001 cagcggcgat gcgtcgaggc gcgagtcgtt aatgactttt tgagtttccg cttccttggc
     3061 aaggacatca gtcgtggcga cgcgcagaat attgtcgctg gtctcaatgc ctagtcggtt
     3121 ggctctaagt atcgcttcca gagcgacgtt ccagggaacg tcttgaacgt tcaccgtcac
     3181 cggcaccgaa cgcacggagg aatcgattac aaagttcaca ccgtattgtt cggtaatgta
     3241 gttgagaatg tcgcggatgt ccgcgtttac aacattcagg ttgatcggct cgcctacaaa
     3301 tcccggttgt ccatactgcc ggccctgatc cgattggctc gcggtgccag tggatatgcc
     3361 gggctttgtt tgcgactgat tctgggcaac ggccggatcc tgattttcag catcatgtgc
     3421 cgccacgatt tcggcggcca gacgcgcttc agcctgcgcc gacagcggga tgtagtttac
     3481 tagtttgagc gtggcacctt tactagttgc cgggcgcgaa gcggtagttc cagccagagt
     3541 gttcgcgccg gcaaggctca aacacaacag gacgagcgcg gtccttttaa tggctgcaag
     3601 aattggtgac tgataaggca tcgttccctc cttaagggaa ttttggacgt gcccgccacg
     3661 cgctacgctc atttg
//
LOCUS       DASUEX010000267         7212 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_626434, whole genome shotgun sequence.
ACCESSION   DASUEX010000267 DASUEX010000000
VERSION     DASUEX010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7212
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_626434"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="VFV61_04500"
     CDS             <1..273
                     /locus_tag="VFV61_04500"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEX4899768.1"
                     /translation="FRSKPKPGYTLEARRNRKQGFVILKVALSSTGKISRVRVLRGLP
                     FGLTESAIKAACKLEFRPAIKDGQSVSQWVTAEYVFRLPDSRLFRP"
     gene            420..2369
                     /locus_tag="VFV61_04505"
     CDS             420..2369
                     /locus_tag="VFV61_04505"
                     /inference="COORDINATES: protein motif:HMM:NF019239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX4899769.1"
                     /translation="MKRFTPFLLSLLILLTVASAREVSAKDNWISVRSRNFQLIGNAG
                     EKDVRQVANRLEQFREVFTRLFPKAKFISPVPTTVIVFKSDKSYSPFRSNPNIAGYFQ
                     PGPDVNYITLTTEVRGEQDPFTVIFHEYTHLLVNSTLGNVPIWFNEGLAEYYSSFAIS
                     DDRKVTLGKPIGHHVYLLRESKMLPLPTLFQVDHKSPHYNERDKQSIFYAQSWALVHY
                     LIQKPGGSDAIDKFMGLMNSNISLDDAFQQSFQTTFEKMETDIRAYIKHDSYPVTVGK
                     FEQKVDFDTQLQVAPLSEADAQAYLGDLLLHSNRTDSEEYLKKALALDANHPMANASL
                     AMLRVREGKMEEARSNLERAVTANSQNYLIHYYYAYALSQEGMDTSHLVSGYSPESLT
                     KMRGELRRAIDLRPDYPESYVLLAFINLVAGSELNETITMVKKILATSPGRNDLALTL
                     AQLYMRTEDFKSAREILERLTQNPQMRAQAQSLLAGVVDYEERLARYRAAKEARGNAA
                     TVNGGPPRLKRGGANDALASEAPGEIPLDPFAYLQEALRLPATGEKQVQGVLLRLDCD
                     AKGIFFVVQLADRVIKLRTSKFENIQITAFTSDAGAEITCGPRQPANPVVICYLPTTD
                     AKAKYDGDLRSVEFVPSDFKLQTKQ"
     gene            2397..2867
                     /locus_tag="VFV61_04510"
     CDS             2397..2867
                     /locus_tag="VFV61_04510"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX4899770.1"
                     /translation="MITCRKMTAPDVTDAFSMLSDFLGSDEHYLASSAAYGDRGIQGL
                     NDALYLFLEHPDLGFVWMAYDEDEVAGVCVICYAVSTSMGSLVAKLDDVSVKSERRGQ
                     GVGTELLVQLKEQLRKEGVSRIDVGVHLENPEARKFYEKLGFIALNEERLACVI"
     gene            complement(2886..4274)
                     /locus_tag="VFV61_04515"
     CDS             complement(2886..4274)
                     /locus_tag="VFV61_04515"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX4899771.1"
                     /translation="MPKELDRDQLFKFAQTHRQEYEDLLQQFVETPTVSCDPAYAEDI
                     RKGVELTVETLRRFGGKADVYQASKGNPVVHAVFGDDKSLPTVTVYNHMDVQPASKET
                     EPWDTEPFVMTRKGNSYFGRGTTDDKGPALAALYGAQAAREAGIPVNIRFLWEFEEEI
                     GSPNFEKIISKARRDLRTDSVVVSDTVWVSRQQPASSAGLRGLAGFLLTLETGTTDTH
                     SGEVGGAARNPIAELMRLICDLYDPTTGRIKIKGFYDDVIPPSKTELDDWRNSGFSVK
                     GFKKAHRLKLMRSQDDLEVMKRIWGMPTMEIHGVVGGYQGPGVKSIVPPRAEVKASFR
                     LVPAQDPKKIQQQLAAAVKQLNPDVQIQFESGAPAFRTVLTGELPQALKRAMKFAFKR
                     DPVFVRDGGTIGAMTSIEQVLKCPVLFLGLSLPEHGYHAPNENFDWEQASGGMVAFAK
                     YFEEIGKLGKLK"
     gene            4752..6458
                     /locus_tag="VFV61_04520"
     CDS             4752..6458
                     /locus_tag="VFV61_04520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX4899772.1"
                     /translation="MRSRQWIPGILFLLLAALVPALIFAQQNRSARRQPGTATDRSAN
                     GTARNKSAVTSAVVKTDLNEALAVIQTNYFDGRKLDYNSVFKSSITGMLKVLDPHSTY
                     FDPIDFAAFKTEQRSEYFGIGATIDHMHQGDQVNTYIRATFDDSPAARAGLRFGDRIM
                     EVDGQSMANKSYPEVRKFLLGPRGTVVKVTVLHNGTGQKETVSITRDAVPLPSIPQAY
                     MVSPGVGYVAMTGGFNLTTNNEFQEALADLHAKGMKMLVLDLRGNRGGLLIQAVKVAN
                     TFLQDGQLIVKQEGRIRGSSEEFIADNDAPDLVPLVVLVNGESASAAEIVAGALQDHD
                     RALIVGENTFGKGLVQLPIQLEYDSALLLTIAKYFTPSGRLIQRDYSHGDFYDYSTRG
                     GIGPDKNANPTAPSGPESRTDTGRTVYGGGGIAPDEIAKPGTITAGEAKLADLLFGFA
                     LELTSGRVKGFENYKVQRPIEFGHDLQASDYPVTDALFAELKRFAAAKPEFKTTPDQL
                     EKFRAHAERQLRYNLTSAAYGTMVAVQVFNNDDKQIARAIEAMPRARELAQSARRARA
                     RS"
     gene            6623..>7212
                     /locus_tag="VFV61_04525"
     CDS             6623..>7212
                     /locus_tag="VFV61_04525"
                     /inference="COORDINATES: protein motif:HMM:NF012981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX4899773.1"
                     /translation="MVDQSNLFIIINNVAARARAAWPCVRQRLQAEGIEFEAAVTSCA
                     GDATLRTRAALRADYRTIAVIGGDGTLSETAEGFFELAQNNSLAQFPTAINPVASLAI
                     LPAGTGDDFARGLMKRRAPLADWLELLVSHCRNLDESSTHAVDVMAAACDADQRQFIC
                     LNASTMGIGGETGARVAAQGKFMRRFSGEVRFLVAAL"
BASE COUNT         1620 a         1951 c         1963 g         1678 t
ORIGIN      
        1 ttcagatcca aacccaagcc ggggtacacg ctggaagcgc gccggaatcg caagcagggt
       61 ttcgtaattc taaaggtggc gctttcgtcg accggaaaga tcagtcgcgt gcgggtctta
      121 cgggggcttc cgtttggcct gacagagagc gccatcaagg ctgcgtgtaa actagagttt
      181 aggccggcaa taaaagatgg tcagagcgtt tcgcagtggg taaccgcgga atacgtattt
      241 cgcctgccgg attcgcgctt attccgaccc tgaggaaatt gccgattgcc aattggcgat
      301 tgccgattgc agattcgcta gtcggaattg aatagtgtaa tcaccgaatc ataaattaag
      361 acctaatcgg caatcgccaa tcgccaacgc caatggatta acaggaccgc actacgatta
      421 tgaaacgctt taccccgttc ctcctctcgc tcctcatttt gttgactgtt gcttccgcgc
      481 gcgaagtctc cgccaaggac aattggatca gcgtgcgctc ccgaaacttt cagttgattg
      541 gcaacgccgg ggagaaagac gtgcgtcagg tggcaaaccg gcttgagcag tttcgcgaag
      601 tgttcacgcg cctgtttccg aaggcgaagt ttatttcgcc cgtgcccacc acggtaatcg
      661 tcttcaagag cgacaaatct tacagtccgt ttagatcgaa cccgaatatt gccggttatt
      721 ttcagccagg gcctgacgtc aactacatca cgctaacgac tgaagtgcgc ggtgagcaag
      781 atcccttcac ggtcattttt cacgagtaca cgcatctgct cgtcaacagc acgctcggca
      841 acgtaccgat ctggtttaat gaagggctgg cggagtatta cagcagtttc gccattagcg
      901 acgatcgcaa ggtcactttg ggcaagccta ttggccatca tgtttacttg ctgcgcgaaa
      961 gcaagatgtt gccgctccca actttatttc aggtagacca caaatcgccc cactacaacg
     1021 aacgcgataa gcaaagcatt ttttacgcgc agtcgtgggc cctggtgcat tacctgattc
     1081 aaaagccggg aggctcagat gcgattgata agttcatggg attaatgaac agcaatatct
     1141 ccctcgacga cgcatttcag cagtcgtttc aaaccacctt tgaaaagatg gagacagata
     1201 ttcgtgctta catcaagcac gacagttatc ccgtgaccgt cggcaagttt gagcagaagg
     1261 tagacttcga cactcagctc caggtggcgc cgcttagcga agcggatgcc caagcctatc
     1321 tcggcgatct acttctccac agcaatcgaa ctgacagtga agaatatttg aagaaagccc
     1381 tggcgcttga tgcgaaccat cccatggcca atgcttcgct cgccatgctt cgcgtgcgtg
     1441 aggggaagat ggaggaagcg cgcagcaatc tcgaaagggc ggttaccgca aactcgcaga
     1501 actatttgat tcactactat tacgcttatg cgctcagcca ggaaggcatg gacacttcgc
     1561 atttagtgtc gggttattct cccgagagcc tcaccaagat gcgcggggag ttgcggcggg
     1621 caatagactt gcggccggat tatccagaat cgtacgtgtt gttagctttc atcaatctcg
     1681 ttgccggaag cgagctaaac gaaaccatca cgatggtcaa gaaaattctt gccacttcgc
     1741 cgggtaggaa cgacctggcc cttacacttg cccagcttta tatgcggacc gaggatttta
     1801 aatcggctcg cgagatactt gagcgcttga cgcagaaccc ccagatgcgc gcgcaagcgc
     1861 agagtctgct ggctggggta gtcgattacg aagagagact ggcccggtat cgtgcggcca
     1921 aagaagcacg cggcaatgct gcgactgtga acggagggcc gccgcgccta aagcgcgggg
     1981 gcgctaacga cgcgctagcg agcgaagcgc cgggtgagat cccgctcgac ccctttgcgt
     2041 atttacaaga agcgctgcgc ctgccggcta caggcgaaaa gcaggtgcag ggagttttgc
     2101 tgcgactgga ttgcgatgcg aaggggattt tcttcgtggt gcaacttgca gaccgtgtca
     2161 ttaagctgcg aacctccaag tttgaaaaca tccagatcac ggcgtttacc agcgatgccg
     2221 gcgccgagat cacctgcggt ccgcgccaac cggcgaaccc cgtggtgatc tgttatctac
     2281 cgactactga tgcaaaggca aagtatgatg gcgacttacg gtcggtggaa tttgttccat
     2341 ctgactttaa gctgcagaca aaacagtaat actgaacctc gacacggagg agcgaattga
     2401 tcacttgccg aaaaatgacc gcccctgatg tcactgatgc ctttagtatg ttaagtgatt
     2461 ttcttggaag tgatgaacac tatttggcga gcagcgcggc gtatggcgat cgcggaattc
     2521 agggactcaa tgacgcgctc tatctcttcc tggaacaccc cgacctcggg ttcgtttgga
     2581 tggcttacga cgaagatgaa gtcgccggcg tttgtgtgat ctgctacgcg gtctccacgt
     2641 cgatgggctc gctggtggcc aagcttgatg atgtctcagt caaatccgaa cgacgcggtc
     2701 agggcgtagg tactgagctg ctggttcagt taaaagagca gttgcgcaag gaaggggtgt
     2761 cgcggattga tgttggtgtg catctcgaga atcccgaggc gcgcaagttt tatgagaagc
     2821 tcggcttcat agcgctcaac gaagaacgcc tggcgtgtgt aatctaagtc ctgatcttat
     2881 tcaggctatt tcagcttacc caactttcca atctcctcga aatacttggc aaaggccacc
     2941 atcccgccgg acgcctgttc ccaatcaaag ttttcgtttg gtgcgtggta gccatgttcc
     3001 gggagcgaaa gaccgagaaa gagcaccggg cacttcagca cctgctcaat actcgtcatc
     3061 gcgccaattg tgccaccgtc gcgcacgaat acgggatcac gtttgaaagc aaacttcatc
     3121 gcgcgcttca gcgcttgtgg caactcacct gttaatacgg tgcgaaacgc cggcgcgccc
     3181 gactcgaact gaatctgcac gtctggattt aactgcttca ctgcggccgc gagttgttgc
     3241 tgaatcttct tcggatcctg ggcgggaacc agccggaacg acgctttcac ttcggcgcgc
     3301 ggaggcacga tcgatttaac tcccggcccc tgatagccgc ccaccacgcc atgaatctcc
     3361 atcgtcggca taccccagat ccgtttcatt acctcgagat cgtcttgcga gcgcatcagt
     3421 ttcaaacgat gcgccttctt gaaacctttg acgctgaagc ctgagttgcg ccaatcatcc
     3481 aattctgtct tgcttggcgg aatgacatcg tcataaaagc ccttgatctt gatccttcca
     3541 gttgtgggat cgtaaaggtc gcaaatcaag cgcattaatt ctgcgatcgg gttgcgagca
     3601 gcaccgccaa cttcgcccga atgcgtgtct gtagttccgg tctctaaggt caaaagaaag
     3661 ccggctagtc cgcgcaggcc tgctgaactt gcgggttgtt ggcgtgaaac ccagaccgtg
     3721 tcagaaacta cgactgagtc agtccgcaga tcgcgacggg ccttgctgat aattttttca
     3781 aagttaggcg acccgatctc ttcttcaaac tcccagagga accgaatgtt caccggaata
     3841 cctgcttcac gagcggcctg ggcgccgtaa agggcagcga gggcaggtcc cttgtcgtct
     3901 gttgtgccgc gtccaaagta actgttgccc ttcctggtca tcacaaaagg ctctgtgtcc
     3961 cacggttcgg tttctttcga agctggctgc acgtccatgt ggttgtagac cgttacggtc
     4021 gggaggctct tatcatcacc gaacacggcg tgcaccaccg ggttgccttt gcttgcctgg
     4081 tagacatccg ctttcccccc aaatcgccgc aacgtttcga ccgtcagctc gacacctttc
     4141 cgaatgtcct ccgcgtaagc cggatcgcag gacaccgtcg gagtttcgac aaattgttgc
     4201 agtaaatcct cgtactcctg gcgatgggtc tgggcgaact tgaagagctg atcgcggtct
     4261 agttccttgg gcattctcga tcctccgatt gagagtatgt ctctggtcac aagaacgatt
     4321 tcgcttgatt aatagcgctg cttatagtgc acaaccttac ggttgttgtc acggtgaaat
     4381 ttttggggaa acgcaccgtt cagggctgtc atcgccgtct cgcagcgctg aaccgaaacc
     4441 gtttaaaccg tttcagtgaa aggtgtcgtc ttcaaacccc agcctgctgc gagaatgaaa
     4501 acaacatttc gagctgaggc cagccgaccc tgcagggcaa ctttttcccg ccaattggtc
     4561 actaaatggg cggtcatgca tttgagccag gccgctcttg ttcgtcagtc cgccaggcga
     4621 actttgtccc aaatcgagcc gtatatttaa gagctttagt tgttcgtgct attctgacaa
     4681 ccctttccgc acccgtcagg gaagggcatt ggcacaggct tccatcgatc agaaccaatt
     4741 aggagggtgg cgtgagaagt agacagtgga tcccgggtat tttgtttttg ctgcttgccg
     4801 ctttagtacc cgcactaatc tttgcgcaac agaatcgatc agctcgccgc cagccgggca
     4861 cggcgaccga tcgttccgct aacggcacag ctcgaaacaa gtcagccgtc acttcggcag
     4921 tcgttaagac tgacctcaac gaagccctcg ccgtaattca gaccaattac tttgacggcc
     4981 gcaagcttga ttacaattca gtattcaagt cttcaatcac gggcatgctc aaggtcctgg
     5041 atccgcactc cacatatttt gatccaatag acttcgcggc cttcaagact gagcagcgtt
     5101 ccgagtattt cggaatcggc gccacgatcg atcacatgca tcagggtgat caagtcaata
     5161 cttatattcg ggctacgttt gacgattcac cggcggcgcg tgcaggtttg cgctttggcg
     5221 atcgcatcat ggaagtcgat ggccaatcga tggcgaacaa gagttatccc gaagtgcgta
     5281 agttcctttt aggaccgcgc ggcactgtgg tgaaagtcac cgtgctgcac aacggcacgg
     5341 gacagaaaga gacagttagc attacacgcg atgccgtgcc gctaccgtcg atcccccagg
     5401 cctacatggt ttcgcccgga gtcggttatg tggccatgac cggtggcttc aacctgacga
     5461 ccaacaatga atttcaggaa gcactcgcag atctgcatgc gaaaggcatg aagatgctgg
     5521 tgctggatct gcgtggtaac cgcggcggac ttctaattca agcggtgaaa gttgccaaca
     5581 cgtttctcca ggacggacag ttgattgtga agcaggaagg ccgcattcgc ggcagttccg
     5641 aggagttcat tgctgacaat gatgctccgg atctcgtgcc cctggtggtg ctggtgaatg
     5701 gtgagtcggc atctgcggca gagatcgttg ccggtgcttt gcaggaccac gatcgagcgc
     5761 tcatcgttgg tgagaataca tttggcaaag gcctggtaca gttgccaatc caacttgaat
     5821 acgattcagc cttgctcctg acgattgcca aatacttcac gccttcgggt cgtctgattc
     5881 agcgcgatta ttcgcatggc gatttttacg attacagcac ccgcggtggt atcggaccgg
     5941 ataagaacgc caatccgacc gcgccttcgg gaccggaaag ccgtactgat actggtcgaa
     6001 cggtttatgg cggcggcggg attgcgccag acgagattgc caagcccggt acgattacgg
     6061 caggtgaagc aaagctcgca gacctacttt tcggctttgc tttagaactc acgagtggtc
     6121 gagtgaaggg ttttgagaat tacaaagttc agcgaccgat tgaatttggt cacgatctac
     6181 aagccagtga ctatccggtg acggacgcgc tgtttgccga gctcaagcgt ttcgctgcgg
     6241 ccaagccgga attcaaaacc acgccggatc aattggaaaa gttccgagcg catgccgaac
     6301 gccaacttcg ttacaacctg acctctgctg cctacggaac aatggtcgcg gtgcaggtct
     6361 tcaataacga cgacaagcag attgcgcgcg cgattgaagc aatgccgcgc gcccgggaac
     6421 tggcacaatc ggctcgccgc gcacgggcac gatcctaatt ggactgcgac atacgaaggg
     6481 acaaaaagac ctgggcggcc aggtcttttt tctttgtcac gcggcttgtt tttccgttca
     6541 caccgggctg cagcgcggtg agaattagat ttccttacgg cacctgttta cgatttctga
     6601 tttaacttct accatccccg ccgtggttga tcagtcgaac cttttcatca tcattaacaa
     6661 tgttgccgcc agggcgcggg ccgcctggcc ctgcgtgcgc cagcgtctgc aagctgaagg
     6721 gattgagttc gaagctgctg taacaagttg cgcgggcgat gccactctgc gcactcgcgc
     6781 cgccctgcgc gctgactatc gaacgattgc cgtcattggt ggcgatggca cgctcagcga
     6841 aaccgctgag ggcttttttg aacttgcgca gaacaattct ttagcgcagt ttccgacggc
     6901 catcaatccg gtcgcatccc tggcaatact tcccgcgggc accggcgatg acttcgcgcg
     6961 tggactaatg aagcgacgtg cgccactcgc ggattggctc gagctgctcg tttctcattg
     7021 tcggaacctt gacgaatcct ctacgcatgc tgtcgatgtc atggcagccg catgcgacgc
     7081 tgatcagagg cagttcattt gcttaaacgc ttcgacaatg gggattggtg gcgagaccgg
     7141 cgcccgggtt gcggcgcagg gaaagttcat gcggcggttt tcgggcgaag tgcggtttct
     7201 cgtagcggcg ct
//
LOCUS       DASUEX010000268         3897 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_731929, whole genome shotgun sequence.
ACCESSION   DASUEX010000268 DASUEX010000000
VERSION     DASUEX010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3897
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_731929"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="VFV61_04530"
     CDS             complement(<1..189)
                     /locus_tag="VFV61_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899774.1"
                     /translation="MHSYRAALLILMSAFFSFVTIFLAPGRTQAQQTQRLVESVDITG
                     NRRLRKDDVLYYVQTRPGD"
     gene            complement(513..2879)
                     /locus_tag="VFV61_04535"
     CDS             complement(513..2879)
                     /locus_tag="VFV61_04535"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEX4899775.1"
                     /translation="MFERYTEKARRVIFFARYEASQFGAPAIEPEHLLLGLMREDKTL
                     TARFFPRAQVSIESIRKEIEGRTLLREKISTSVELPLAPETKRVLAYAHEESDRLQHR
                     HIGTEHLLLGLLREERSMAAEILYERGLRLNAVRDEIARQSGADARGSQKKDTPHLVE
                     FSRDLTDDAANDKLDPLIGREAEIERLVQILCRRTKNNPVLIGEPGVGKTAIVEGLAQ
                     RIVRGEVPSFLEHKRILSLDLSLIVAGTKYRGQFEERLKQIMRELIENPQYIVFIDEL
                     HTLVGAGSAEGSLDAANILKPALSRGEIQCIGATTPGEFRKSIEKDRSLERRFQAVKV
                     PPPSEAEAVEILNGVRERYESFHQVRYTDDSLQAAVTQSHRYIPDRFLPDKAIDVIDE
                     AGARVKLRVRREQGNLADWNQLNEWSRSYDENQSVRESSEDALVSAEVTRDDVEEVIA
                     RWTGIPVTSLKEEETRKLLRIESELHTRVVSQRPAISALARAIRRSRAGLKNPLRPVG
                     SFLFLGPTGVGKTEVARSLAHFLFGSERSMIRFDMSEFMEKHSVSKLIGSPPGYVGHE
                     EGGQLTERIKRAPYSVLLFDEIEKAHPDIFNILLQVFEDGLLTDALGNAIDFKNAIII
                     MTSNIGARFIQKKGTMGFQGTAEASRDKLEEMVMSQVRQTFNPEFINRLDEIIIFDQL
                     LDDELLEVVQLQIEQMNRTMISHGLEVRLTDEAKRWIVDKTCADRSYGARPLRRALQK
                     YVEDPLSEAMIQGQLGRAGLIEVSVEGDELTHRPVDIETADDDVLAIH"
     gene            complement(3050..3742)
                     /locus_tag="VFV61_04540"
     CDS             complement(3050..3742)
                     /locus_tag="VFV61_04540"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4899776.1"
                     /translation="MDVGLMVTDLRKSFPAPSGHKLDVLRGISFSVQPGQSVAIVGAS
                     GAGKSTLLNLLGGLEAPDHGSIALGKFAIDCARSSELATFRRNHIGFVFQFHYLLGDL
                     SALENVALPLWIGRKSRPAALEQALASLADMGLKDRGDHPVSHLSGGEQQRVATCRAL
                     IKQPALVLADEPTGNLDAAAAQELGEHLISYAQRHRSIVVIATHNEKLAQLCNQTLRI
                     DEGRLKVVSDPN"
BASE COUNT          889 a         1050 c         1052 g          906 t
ORIGIN      
        1 gtcaccagga cgggtttgaa cgtaatagag cacatcgtct ttccgcaaac gacgattccc
       61 tgtgatatca actgattcaa cgaggcgctg tgtttgttga gcttgcgtgc gtccgggcgc
      121 aagaaagatg gttacaaagg agaaaaatgc gctcatcagg atgagcagcg cggcgcgata
      181 tgagtgcatg gaagccaatt ccctcagtgg ttcttaccgg acctcagata cttcggggag
      241 tcgtatgact tagataaaat taagttttac gagaaaaata taaggccaaa tgccgaacgt
      301 tacctgccgg acaagcccta aagccttagt ggaactgttg gattcagaag cgtcagcgac
      361 acgttgccac tccgaataat acttgtccag caaagtcgcg cattataccg aaccaacgcg
      421 aaaaaagtaa aaaggcacaa gaaaaggtaa ggctggggga aattattcgg ccccagcctt
      481 ttatcctttg ctctgccttc gctatggggg aattaatgga tggccaaaac gtcgtcgtcg
      541 gccgtctcga tgtccacagg tcgatgagtt agttcgtcac cttccacgga gacctcaatt
      601 agaccggctc gacccaattg accctgaatc atcgcctcgg agaggggatc ttccacgtat
      661 ttctgtaagg ctcgccgtaa gggccgggcg ccatacgagc ggtcggcgca ggttttatca
      721 acaatccagc gcttcgcttc gtctgtcagc ctgacctcca ggccatggga gatcatcgtg
      781 cgattcatct gctctatctg gagttgcacc acctcaagca gctcgtcgtc cagcaattgg
      841 tcgaagatga tgatttcgtc gaggcgatta ataaattccg gattgaatgt ctgccgcacc
      901 tgggacatca ccatttcttc cagcttgtcg cgtgaagctt cggcggtgcc ctggaatccc
      961 attgtgccct tcttttgaat aaagcgagcg ccgatgttcg acgtcatgat gatgatggca
     1021 tttttgaagt cgatagcgtt gcccagagcg tcggtaagca atccatcttc aaagacctgc
     1081 aacaggatgt tgaagatatc cgggtgcgct ttttcgattt catcgaagag caaaactgag
     1141 tagggcgcac gcttgatgcg ttcagttagc tgaccgcctt cttcgtgacc aacgtatccg
     1201 ggaggcgaac caatcagttt cgacaccgaa tgtttttcca tgaactcaga catgtcgaaa
     1261 cgaatcatcg aacgctcact gccaaacaga aaatgcgcca gtgaacgcgc cacttctgtt
     1321 ttgccgacgc cggtgggacc aaggaacagg aacgagccaa ccggtcgcag cgggttcttc
     1381 aacccggccc gcgagcggcg aatcgcccga gcgagcgcgg agatggctgg acgttgcgat
     1441 acgacccggg tgtgcagttc actttcaatg cgtagcagtt tgcgcgtctc ttcttctttc
     1501 aacgaagtta ccggaatgcc agtccagcgc gcgattactt cttcgacgtc atcgcgcgta
     1561 acttctgccg aaaccagcgc atcttcgctg ctttcgcgca cactctggtt ctcatcataa
     1621 gagcgcgacc attcgttgag ctgattccag tcagcaagat tgccctgctc gcggcgaacg
     1681 cggagtttta cgcgcgcgcc cgcttcatca attacgtcaa tggctttgtc cggtaaaaag
     1741 cggtcgggaa tatagcggtg cgattgcgtg acggccgctt gcagcgaatc atccgtgtag
     1801 cgaacctggt gaaaactctc atagcgctcg cgcactccgt tgagaatctc aacagcctca
     1861 gcttccgacg gcggcggcac tttcactgcc tgaaaacgac gttccagtga gcgatccttt
     1921 tcgatcgact tgcgaaactc gcctggagta gtggcgccga tacattgaat ctcgccgcga
     1981 gacaaagccg gcttcaaaat atttgcagca tcgagcgagc cttccgcgga tccggcgccg
     2041 acgagtgtgt gcagttcgtc gatgaaaaca atgtactgcg gattctcgat cagctcccgc
     2101 atgatctgtt tcaaccgttc ttcaaactga ccccggtatt tagtgccggc gacgattagc
     2161 gagaggtcga gagaaagaat tcgtttgtgc tcgaggaatg aaggcacttc accgcgcacg
     2221 atgcgttggg ccaggccttc aacaatggca gtcttgccga cgccaggctc gccaatcagc
     2281 acgggattgt ttttggtgcg gcgacaaaga atctgtacta gccgctcgat ttcagcttcg
     2341 cggccaatca acggatcaag tttgtcattg gcagcgtcgt cggtgaggtc gcgggaaaac
     2401 tcaaccaggt gcggcgtgtc cttcttttgc gaaccacgcg catccgctcc ggactgtcgg
     2461 gcaatttcgt cgcgcacagc attcaaccgc aagccgcgtt cgtaaagtat ctcggcagcc
     2521 atggaacgct cttcgcggag caagcccagc aatagatgtt cagtgccgat gtgacggtgc
     2581 tggagccgat cgctctcttc atgagcgtag gccagcactc gctttgtttc gggtgcgagc
     2641 ggtaactcca cagacgtgga aattttttcc cgcaagagtg tgcgtccttc aatctccttg
     2701 cggattgatt cgatggaaac ctgggcgcgg ggaaaaaagc gagcagtgag ggtcttatct
     2761 tcgcgcatta atcctaagag caggtgttcc ggttcaattg ccggagcgcc aaactgggac
     2821 gcctcgtagc gcgcgaaaaa aattacccgg cgtgcctttt ctgtatagcg ttcaaacatt
     2881 gggcgttctt gtctgcggat acctatatga ttaccgtgcc ggggatcatc atgccgggcg
     2941 aaacttcagc ccggcacttg ccccgcatta taacccatga agcatagtgc caaaagtaat
     3001 tgcttgtttc tgcttcttta aaacggctaa ccgttgcagt atttcaggtt caattcggat
     3061 ctgacacgac tttaagtcgc ccctcatcaa ttctaagagt ttgattacaa agttgcgcca
     3121 gtttttcatt gtgggttgcg atcaccacga tagaacggtg acgctgggca tacgaaatga
     3181 ggtgctcccc gagttcctga gcggccgctg cgtcgagatt accagtcggt tcgtcggcta
     3241 aaacaagcgc aggctgctta atcagtgccc ggcaagtggc aacgcgctgc tgttcgccgc
     3301 cggagagatg gctcactggg tgatccccac gatctttcaa tcccatatca gccagagatg
     3361 cgagcgcctg ttcgagtgcc gccgggcgac tctttcttcc gatccagagt ggcaaggcca
     3421 cgttctccag ggcgcttaag tctcccagca ggtaatgaaa ctgaaaaaca aatccgatgt
     3481 ggttgcggcg gaaagttgcc agttctgatg atcgggcgca gtcgattgca aactttccca
     3541 gcgcgatgga gccgtgatcg ggcgcttcca gaccaccaag caaattcaac agggtggact
     3601 ttccagctcc ggaagcgccg acgatggcca cgctttgacc cggctgcaca gaaaacgaaa
     3661 ttccgcgcag cacatcaagc ttgtggccgc tcggagccgg gaagctcttg cggagatcgg
     3721 tgaccatcag tccaacgtcc aatgtcccat gtccaaggtc actgctttcc ttcacggacg
     3781 ttggactttg gacattagac tttggacaag attggggact tcggactttg gacattggac
     3841 tagtttgtgt cccgcagcat ttccacaggg cgcacctgtg ctgcggcgcg cgccgga
//
LOCUS       DASUEX010000269         3018 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_731960, whole genome shotgun sequence.
ACCESSION   DASUEX010000269 DASUEX010000000
VERSION     DASUEX010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3018
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_731960"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2555
                     /locus_tag="VFV61_04545"
     CDS             <1..2555
                     /locus_tag="VFV61_04545"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HEX4899777.1"
                     /translation="LRRLAAGAFLCLAFTFAGATLAVVDRDKHANDLGVLLDQGAVIA
                     GAPVELTGVLTQQPEAAPEAFYLTLRVETLSWKGEEQLTAGLVSLLLPLSGKAAKNEY
                     DKLELRYGARIRTLTTLRRSETFRNPGGTSFTEYLERKGLAATAFVKSPQLIERLDDE
                     RVLLPLAWLYDWRQQLQQAINTHFAPDTAGILNASLLGNHHFLSRSAAEKFREGGTFH
                     VLVISGLHISFIGGLIFLIAGRVTRNKVIQFLLSATILWSYSFAVGAEASVVRAALMF
                     TFIAFAPVMARRAASLNALGAAALVLLTMRPRDLFDPSFQLTFLSVTAIVVFAWPLLR
                     RMSEIGSWRPTRASPYPPHCRRWLRVFCEALFWSDRKWRRELSQLNYRCKLLKTPIAE
                     RFERFHLQAIFRYGFAAMLVSCCVQITMLPLLVVYFHRVSISGLVLNIGVSAIMGVLI
                     FSGMAALLVAPLSMSLSAPLIKVTDLWQRLMVHSVDPFAHLGFASVRLPEYSGSSAAV
                     YVVFFVPLIILALTLPRWNPLARPNPTATSSRWRLLIKIAATAQVLLVGIVLFHPLSV
                     ANPDGKLRVDFIDVGQGDALLLTMPDGTTLLVDGGGRPGFFKKKSEGEPTESFVRDTR
                     SIGEAVVSEYLWWRGLDQVDYVLATHADADHMSGLNDVVRNFKVRAALVARTPENDPE
                     FLEFLVSTRNAGSPLQLIGGGDSLRFGAVRIAVLWPPASDDHQARSANNDSLVLQVEY
                     GVRRVLLLADIESKAELSLLQQPHETLQADIVKVAHHGSRTSSTSGFVAAVHATTAII
                     SVGQQSMFGHPHQQVVERWRAAGANVLTTGQSGTITVTTDGRELEVTTFVRE"
     gene            complement(2696..>3018)
                     /locus_tag="VFV61_04550"
     CDS             complement(2696..>3018)
                     /locus_tag="VFV61_04550"
                     /inference="COORDINATES: protein motif:HMM:NF017778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cysteine dioxygenase family protein"
                     /protein_id="HEX4899778.1"
                     /translation="WRPGQRTPIHDHNGSQGAVMVYEGLMWETTFNFDSKSGLSYKSG
                     REYGPGKVTDAAVPDIHQLGNPDVSGQDLITIHIYSPPLGVLKTYKVGSPDIDLYTPN
                     DFPA"
BASE COUNT          597 a          814 c          870 g          737 t
ORIGIN      
        1 tgctgcgcag gttggcggct ggcgcgttct tatgcctggc gtttactttc gctggagcca
       61 ctctggcagt agtcgatcga gataaacacg cgaacgactt gggcgtcctc ctagatcaag
      121 gagcagttat cgccggcgca cctgtggaac tcaccggtgt gctgacacag caacctgaag
      181 ctgcgccgga agccttctat ctcacgctgc gcgtcgagac gttgagctgg aagggtgaag
      241 agcaactcac cgccggctta gtctcgttgc tgttgcccct ttcggggaag gcggcgaaga
      301 acgaatacga caaacttgaa ttacgttatg gcgcgcgcat tcgcacgctg accactctaa
      361 gacgaagcga aacttttcgg aatcccgggg gcacttcgtt tacagagtac ctggagcgca
      421 agggcctcgc ggcaactgcc ttcgtgaaaa gcccgcagtt gatcgaacgg ctcgatgacg
      481 agcgcgtgct gctcccgctt gcgtggctct acgactggcg ccagcaattg cagcaagcga
      541 tcaatactca cttcgctcct gatacagccg gcattctgaa cgcctccctt ttggggaatc
      601 accacttcct ttcacgttcg gccgcggaaa agtttcgcga aggcgggacc tttcacgttc
      661 tcgtaatcag cggattgcat atcagcttca tcggtgggct gatattcctg attgcggggc
      721 gcgtcacgag gaacaaagtg attcaattcc tgctctcggc cacgatcttg tggagttact
      781 ccttcgcggt gggcgcggaa gcgtcggtag tgcgcgcggc gttgatgttt acattcatcg
      841 ctttcgctcc ggtaatggct cggcgcgcag cctcattgaa tgcgctcggg gcggcggcac
      901 tcgtattgtt gacaatgcgc ccgcgagatc tttttgaccc ttcgtttcaa ctgacattcc
      961 tttcggttac tgcgatagtg gttttcgcct ggccgttgct gaggcgaatg tcagaaatag
     1021 gttcctggcg tcccactcgc gcgagccctt atccaccaca ttgccggcgc tggttgcggg
     1081 ttttttgtga ggcactgttt tggtcggata gaaagtggcg gcgcgaactg tcgcagctca
     1141 attaccgttg caagctcttg aagacgccaa tcgccgaacg attcgagcgc tttcacttac
     1201 aagccatctt ccgttacgga ttcgccgcga tgctggtttc ctgctgtgtc cagatcacga
     1261 tgctgccgtt gcttgtcgtt tactttcacc gcgtttcaat cagcggcctt gttttgaaca
     1321 tcggggtaag cgcaatcatg ggagtgctca tcttcagcgg gatggcggcg ctactcgtcg
     1381 cgccactgag catgagtctt tccgcaccac tgatcaaggt caccgattta tggcagcggt
     1441 tgatggtgca cagcgttgat ccgtttgcgc atttgggttt cgcctcggtg cgtcttccgg
     1501 aatactcagg cagctcggcc gctgtctacg tcgtgttctt tgtgccgcta ataatcctgg
     1561 cgctaacttt gccacgctgg aatccgctgg cgcgtcccaa tcccacggca acgagctctc
     1621 gctggcgatt gctcatcaaa atcgcggcga ctgcgcaggt gctgctcgtc ggtatcgttt
     1681 tgtttcatcc gttgagtgtt gcaaatcctg acggcaagct gcgcgtcgac tttatcgatg
     1741 ttggccaggg agatgcgtta ttgttgacga tgcccgatgg gacgactttg cttgtcgacg
     1801 gcggagggcg tcctgggttc ttcaagaaaa aatctgaagg cgaaccgact gagagtttcg
     1861 tacgtgatac ccgttcaatt ggcgaggccg tggtttcgga atatctctgg tggcgcgggc
     1921 ttgaccaggt cgattacgta ttggcaacgc acgcggatgc agatcatatg tcagggctga
     1981 acgacgtggt cagaaatttc aaggtccggg ccgccctggt agcgagaaca ccggaaaacg
     2041 atccggagtt tcttgaattc ctggtttcaa ccaggaacgc aggatctccg ttgcagctaa
     2101 taggaggtgg cgactcgtta cgctttgggg cagtccgaat cgccgtgctc tggccgcctg
     2161 cttctgatga tcaccaagcg cgatctgcca acaacgattc ccttgtgctg caagttgaat
     2221 atggcgtccg gagggttttg ctcttagcgg atatcgaaag caaagcggaa ctgtctcttt
     2281 tgcagcagcc tcacgaaacc ttgcaggcag acatcgtcaa ggttgcgcac catggcagtc
     2341 gcacctcttc aacttccgga tttgtagccg ccgtgcatgc cactacagcg atcatttctg
     2401 tcgggcagca gtcaatgttc ggtcatccgc accagcaagt cgttgaacgc tggcgcgccg
     2461 caggcgcgaa cgtcttgacc acgggccaga gcggaacgat caccgtcaca accgatggtc
     2521 gagaattgga agtgacaacg tttgtaagag agtgaacgat gccgatttcc aattgccaat
     2581 tgccaactgg catgtcgcgt ccgagctgtg tcacggtttc agtgaagtca gtatcgcgac
     2641 atgtcgggaa acgggtgggc actgtctaat cggcaatcgc caatcgagaa ccgacttaag
     2701 cagggaagtc gttgggggtg tagagatcaa tgtcgggact gccgactttg taagtcttga
     2761 ggacgccgag tggcggcgag tagatgtgaa tcgtgatcaa atcctggcca ctgacgtctg
     2821 gatttcccag ttgatgaata tcgggcaccg ctgcgtcggt aactttaccg ggaccatact
     2881 cgcgtcctga tttgtaggac agcccggact tggaatcaaa gttgaacgtt gtctcccaca
     2941 tcagaccttc ataaaccatt accgcccctt gcgagccgtt gtgatcgtgg atcggtgtcc
     3001 gttgtccggg tcgccaac
//
LOCUS       DASUEX010000270         8045 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1543961, whole genome shotgun sequence.
ACCESSION   DASUEX010000270 DASUEX010000000
VERSION     DASUEX010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8045
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1543961"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(219..2147)
                     /locus_tag="VFV61_04555"
     CDS             complement(219..2147)
                     /locus_tag="VFV61_04555"
                     /inference="COORDINATES: protein motif:HMM:NF026220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HEX4899779.1"
                     /translation="MTTTASVNAQGLEMRAWQWQNPLPQGNSINNVKFAADRRNGWAV
                     GGDGAILRSRNGGFAWEEQISPTNTTLYGLYLRDKARLIAAGSGGVILTTTNGGRRWV
                     RRATGTRDHLFCVTFAPGASQQGWAVGSFGAIVHTKDGGITWQLQKSPTTAHLFSVAF
                     ASEQTGIAVGARGTLLVTSNGGVDWKALTGISGKALTGVTFSSPLRAVVVGYAGTILE
                     TRDAGVSWQAAQSLTTTDLLSVYFKDDKMGWVTGDDGLVLTTADGGSIWLPVSVRTSP
                     RLSSVHFADESLGWAAGADGLILRTDDGGLSWRRLTEGGRDDLAQIVFINDTRGWAVG
                     ARGKILYTSSAGRSWTVRPSGTTARLNAVDFVNEKFGWVAGDQGTMLRSINGGVTWAQ
                     VRIEANEDLLGVDFISPTQGWAVGARGLILETRDGGQTWHFQRADTFNWLEDVRFVDA
                     RHGVAVGSNGTILRTEDGGTSWKRVDRNLKEWLYAVDFADAQRGYAVGAQGLILRTDD
                     GGLTWKDMESGVTTNLFAVAVSTRDELMVTGEQGRILESKDGGETWLTQPTITSTTLF
                     SIANRGGSNVWVAGRGGAILRRTDDVETVRLPKPGLPPLLRRSTPKLQSQNSQTPLVI
                     DDGDIPRAVPTIKKPARP"
     gene            complement(2364..2897)
                     /locus_tag="VFV61_04560"
     CDS             complement(2364..2897)
                     /locus_tag="VFV61_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899780.1"
                     /translation="MSEKGFRRNNFFAGRLLAAADLELEQNYFRDKQRLHNRALHGFG
                     IVSGLEVSRRGDKLQITSGLALDCEGNEIVVAESHSHPLPPLISETSVFLVVHYREQQ
                     TNPTPSPGLDESFEPALIEEGFTLAFESSNPNRSHRHKQGRWQACGKAHGLVLARLRN
                     TSGRWRLDRRLRRPTVK"
     gene            complement(2906..3472)
                     /locus_tag="VFV61_04565"
     CDS             complement(2906..3472)
                     /locus_tag="VFV61_04565"
                     /inference="COORDINATES: protein motif:HMM:NF012304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase H family protein"
                     /protein_id="HEX4899781.1"
                     /translation="MTTAACVLGYVSNDHETSAMTKTAGEHKAHKAPKKQVTIFCDGS
                     SLGNGKAETRAAAVALLGYQGIWRSVGCYLGQATNQQAEIAAAAVGLEALRQPCMVRL
                     FTDSRYVVETMNGRFRRKTNHDWWARLDKAADRHKISWEWTAGHAGHVIQEAVDRAAR
                     RLATLGRVEPEVLRDAEEKVGTLDPFHE"
     gene            3676..4167
                     /locus_tag="VFV61_04570"
     CDS             3676..4167
                     /locus_tag="VFV61_04570"
                     /inference="COORDINATES: protein motif:HMM:NF033537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lasso peptide biosynthesis B2 protein"
                     /protein_id="HEX4899782.1"
                     /translation="MRLFHLIARGSRKIIHKPGEAFLIFRMALWVSWLSVLVKLRPLP
                     RALELVAAKPRPATNQGATSEETMNRLAGAIDLLLRTDLLVFKPVCWKRAAVLHRYLT
                     LSGINSKIVFGVRRGAQGQVDGHAWLESNGAPILETTAPNYKVTYAFPSDDPFEADLA
                     SMS"
     gene            complement(4262..5578)
                     /locus_tag="VFV61_04575"
     CDS             complement(4262..5578)
                     /locus_tag="VFV61_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HEX4899783.1"
                     /translation="MLKSMQYRLPLIAALVFSSFLCTVQTIRAQKSLNGHQSSVKVKE
                     SPDAERISKDVPAPNVATVGQLVISEFRVRGPNGLNDEFIEIFNNSGADHTVAGGGTG
                     YAVAASNGVARCVIPNGTVIPNRGHYLCVNSVGYSLASYPAGNGTTATGDATYTTDIP
                     DNAGIAIFNTSVAANFTLANRLDAVGSTSEANTLYKEGTGYPALTPFSIDYSFYRDNC
                     GKGGSITTFGNCPSFGNLVDTNNNAADFIFVDTNGTSAGAGQRLGAPGPENLSSPIQR
                     NASMKPVLLDPCVSSASPPNRVRDFTSDPPNNSTFGTVDIRRTFINQTGGNVTRLRFR
                     IVDQTTFPAPSGTADLRHRTSSAVVVTVDRPPCGSGTSNVTVQGTTVEAPPSQPNGGA
                     FNSSWSAGTVTLATPLANGASIDIRFLLGIQQTGNFKVLLNIEALP"
     gene            complement(5926..>8045)
                     /locus_tag="VFV61_04580"
     CDS             complement(5926..>8045)
                     /locus_tag="VFV61_04580"
                     /inference="COORDINATES: protein motif:HMM:NF014544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HYR domain-containing protein"
                     /protein_id="HEX4899784.1"
                     /translation="TTAGLVGTLQTSGGITPVGNPNRTYGVITPGSPPVSQPYTFSVA
                     GACGSTVTATLHFQDGIVDLGTVTFQFTLGTSTNSTQTFSNATAIVIPASGTGATTGS
                     PATPYPSNITVAGFTGPVSKVTVTLKNISHTFPSDVDVLLVGPTGVKFVLMSDVIGGT
                     DWTGQTYTIDDAAAALFPASGSPPASGTFQPTNYGTGDLFPAPAPAAPYLDPATAGSA
                     TLASAFNGLSPTGTWSLYVVDDAGTDIGSFAGGWELTLTSSGAVCNTQACSITCPTNI
                     TVPADGGGTSAVVNYPNPAVTGACQTISASPASGSTFPVGTTTVNVTTGAGPACSFTV
                     TVTGGTPPLASTTLAIGEFRLRGALGALDEYIEIVNVSGAPFTVNASDASAGWSVAAL
                     NAAGTVASIVATIPNGTMIPARGHYLIANNTASVGYSLSNYGGAGNATPNATYTADIA
                     DNTGVALFNTANVANLSAATRIDTVNFTSQNGAQSPLFTEGTRLAPIAGDGEYAFVRK
                     LTTGFPKDTGDNAADFEFVSTTAGTFGVLSVRGAPGPENLASPIQRNATVKAGLIDPT
                     QSSSAPPNRVRDTTPGTGATALGTLDIRRKFTNNTGAIITRLRFRAVDITTTNTPGGP
                     LADLRILTSVDTTANGGTITIRGTALESPSDAVTGGGSNSSVVVAIPGGFLAPGASVN
                     VRYLLGVAQGGTFRFFVNVEALP"
BASE COUNT         1774 a         2238 c         2225 g         1808 t
ORIGIN      
        1 ggccggcagc agtttgcggc tcgtgaggtc gccggtggcg ccgaagatta cgacaacgca
       61 aggttcggag gttcgctcca gacgcgttcc actacgtaag ggatttacgt tctcaaccaa
      121 tgttgtcatt tggagttttt gcgctttgaa ctttgtgctt tgaactttga attttgaacc
      181 tgctcctgtc tcctgcctcc tgactcctgc ctaatgttct acggccttgc cggtttcttg
      241 atcgtaggaa cggcccgcgg aatgtcgcca tcgtcgatga cgagcggtgt ctgggagttc
      301 tgtgattgaa gcttaggagt actgcggcgc aacaggggag gcagaccagg tttcggtaag
      361 cgaaccgttt ccacatcatc agtgcgcctg aggatggcgc cgccccggcc tgcgacccaa
      421 acgttcgagc cacctcggtt cgcaatcgag aacagggtcg tgctggtgat ggttggctgt
      481 gttagccacg tctcaccccc atctttgctt tccaaaattc ttccctgctc gccggtcacc
      541 atcaattcat cacgcgtgga cacagccacg gcaaacaagt tcgtcgtcac cccgctttcc
      601 atatctttcc aggtaagtcc gccatcatcc gttcgcagaa tcagaccttg cgcgcccacc
      661 gcatatccgc gctgcgcgtc cgcgaagtcg accgcgtaga gccactcttt cagattgcga
      721 tcaacgcgtt tccaggacgt gcctccatcc tcggtgcgca ggatcgttcc atttgaacca
      781 accgcgacgc cgtgccgtgc atcgacgaag cgaacgtctt cgagccagtt gaaagtgtca
      841 gcacgctgga agtgccaggt ttgaccaccg tccctggtct cgaggatgag gccccgcgcc
      901 ccgactgccc agccttgcgt cggcgaaatg aaatcaactc ccagcagatc ctcgttcgcc
      961 tctatccgaa cttgagccca ggtgacaccg ccgttaatcg aacgcagcat cgtgccttga
     1021 tcgcctgcca cccagccgaa tttctcgttg acgaaatcaa cggcatttag gcgcgcggtg
     1081 gtgcccgaag ggcgaaccgt ccagctacga ccggccgaac ttgtgtacag aatttttccg
     1141 cgagcgccga cagcccaacc gcgcgtatcg tttatgaaaa cgatctgtgc gagatcatcg
     1201 cgacctcctt cggtgagacg acgccacgaa agtccgccgt cgtcggtgcg cagaatcaac
     1261 ccatcagccc ccgctgccca accaagcgac tcgtctgcaa agtgcaccga cgaaagcctg
     1321 gggctggtgc gcacactgac gggaagccaa atcgaacctc cgtctgccgt cgttagaacg
     1381 aggccatcat caccagtcac ccagcccatt ttgtcgtctt tgaaatagac cgagagtaaa
     1441 tctgttgtcg taaggctctg cgctgcctgc cagctaacgc ccgcatcgcg agtttccagg
     1501 atcgttcccg cgtacccaac gactacggca cgcaaaggag aactaaaagt gacaccggtg
     1561 agggctttgc cactgattcc ggtaagtgct ttccaatcga caccgccgtt gctcgtcact
     1621 agtagcgtgc cccgggctcc tacagcgata ccagtttgct cgctggcgaa ggcaacagaa
     1681 aaaagatgag cggtggtcgg tgacttttgt aattgccagg tgatgccgcc gtcttttgta
     1741 tgaacgatgg ccccgaacga tcccacggcc cacccttgct gggaggcccc gggagcgaag
     1801 gtgacacaaa aaaggtgatc tcgcgttcca gtggcgcgcc gcacccacct gcggccgccg
     1861 tttgttgtag taaggatcac gccacctgag cccgcagcga tcagccgcgc tttgtctcta
     1921 agatagagtc cgtacaacgt tgtgttggtt ggtgagatct gttcttccca ggcgaatcca
     1981 ccattacgag agcgcaaaat cgcgccatca ccgccgacag cccaaccgtt cctgcggtcg
     2041 gcggcaaact tcacattgtt aattgagttt ccctgtggca aagggttctg ccactgccag
     2101 gcccgcattt ccagtccctg ggcgttaacg cttgcggtgg ttgtcacgca gagcaggagt
     2161 aagagagtcc aggttacagc acggcgtgtc gcgcgccaca taggttcgct gcctggaatt
     2221 gctgagataa aagacaatct tgtccgctgt aagagcaaag tgagtagagc tacaactttc
     2281 ggggagtaac agtctctgga gcacaggatt ataaagatga aggctcggga aagcgaatag
     2341 cactggaggt aataaagccg cggctattta actgtaggcc gccgcaaacg gcgatcaagc
     2401 ctccatcgtc cggatgtgtt tcgcaagcgg gctaaaacta atccatgagc ctttccacat
     2461 gcctgccaac gtccctgctt gtgccggtga ctccgattcg gattactgct ttcaaaagcg
     2521 agggtaaaac cttcctcaat caaagccggt tcaaacgact cgtcgaggcc cgggctgggc
     2581 gtgggatttg tttgttgctc gcgatagtgg acgaccagaa aaacggaggt ttccgaaatt
     2641 aatggcggca aaggatgcga gtgcgattcg gcaaccacga tctcattgcc ttcgcaatca
     2701 agcgcgaggc cgctcgtgat ttgcaatttg tcgccacggc gcgagacttc gagacctgat
     2761 acgatcccga atccatgcag agcgcgattg tgcagtcttt gtttgtcgcg aaaataattc
     2821 tgttctaatt ccaaatctgc cgccgctaac aaacggccgg caaaaaagtt gttacggcga
     2881 aagccttttt cactcatggc tttggttact catgaaaagg gtcaagcgtg cccacttttt
     2941 cttccgcatc acgcaacact tctggttcaa ctcggcccag cgtagcgagg cgacgtgccg
     3001 cacgatccac cgcctcctga atcacatgac cagcgtgacc ggcggtccac tcccaactga
     3061 ttttgtgacg gtctgccgct ttgtcgagac gcgcccacca gtcatgattt gttttccggc
     3121 gaaagcggcc gttcattgtt tcgacaacgt agcgtgaatc tgtaaagagc cggaccatgc
     3181 aaggttggcg caaggcttca agaccaacag ccgccgccgc gatctccgct tgctgattgg
     3241 tagcttgtcc gagataacac ccaactgaac gccaaattcc ctggtagccg aggagggcca
     3301 cagccgctgc cctcgtctcg gctttaccat tgccaagact cgaaccgtca caaaaaatcg
     3361 tgacctgttt ttttggcgct ttgtgtgcct tgtgttcgcc tgcggtctta gtcatcgcgg
     3421 aagtttcgtg atcgttggaa acgtagccta acacgcacgc tgcagtggtc atagttctga
     3481 ttgccagggc tgaactttca gttaatgcac gaatttgcgc aggcgcgtcc cctgggcctc
     3541 cctgcaaacg aatgtgcgtc gctgtaaact aaaatgtctg tttgcgtctg cgcgatgaat
     3601 ccgttcgctc gaagtcgacc gcatcggcta gaattctaac ggccggagaa actaatcaga
     3661 cttatcctga aggcgatgcg actgtttcac ctgattgcgc ggggctcgcg caagatcatt
     3721 cataaacctg gcgaggcgtt cctgatattc aggatggcgt tgtgggtgtc ctggctttcg
     3781 gtgctggtaa aacttcgacc cctgccgcgc gcgctcgagc tggtcgcagc taaaccaagg
     3841 ccggcaacta atcagggtgc gacctcagaa gaaactatga atcgactagc gggtgcgatt
     3901 gatttgcttt tgcgaaccga tctcttggtc tttaagccag tctgttggaa acgtgcggcc
     3961 gtgttgcatc gctacctgac tcttagtgga atcaactcga aaattgtctt cggcgtcagg
     4021 cgcggtgcgc agggccaagt cgatggtcat gcctggctcg aatcaaacgg cgcgccgatt
     4081 ctcgaaacca ccgccccgaa ttacaaagtc acttacgctt ttccttccga cgaccccttc
     4141 gaagccgatc tcgcttcaat gtcgtagctc aaagaaaaaa gagaggagcc gaagctcctc
     4201 tcctttgtca atcccatcga tttcgcgtta cgaactctct gaacccgaaa ctcgaaacta
     4261 actatggtaa cgcttcgata ttcagcagca ctttgaagtt tccagtctgt tgaatgccga
     4321 gtaggaaacg aatgtcgata ctagcgccgt ttgcgagggg agtagccaaa gtcacggttc
     4381 ctgcggacca agatgagttg aaagcaccgc cattcggctg agatgggggt gcttcgaccg
     4441 ttgtgccctg caccgtaacg ttgcttgtac ccgagccaca aggaggtcga tccaccgtta
     4501 ctactactgc cgagcttgtt cggtggcgca gatcagcggt tcccgaaggc gccgggaagg
     4561 ttgtttggtc aacgatgcga aaacgcagcc gagtcacatt tcccccagtc tggttgatga
     4621 acgtccggcg aatatcgacc gttccaaaag tggaattgtt tggaggatcg ctagtgaaat
     4681 cccgcacgcg gttcggagga gaggctgaac ttacgcacgg atcgagaagc acgggcttca
     4741 tcgaggcatt gcgttgaatt ggactggata gattctcggg tcccggagca ccgagacgct
     4801 ggcccgcccc cgccgaggtg ccgttggtat caacgaatat gaaatcagcg gcattgttgt
     4861 tggtatctac cagatttcca aaggaagggc agtttccaaa ggttgtgatt gaacccccct
     4921 taccgcagtt gtcgcgatag aaagaataat cgattgagaa gggcgttaat gccgggtaac
     4981 ccgtgccttc cttgtaaagc gtgttggcct cactggtgga accaaccgcg tcgagcctgt
     5041 ttgccagcgt gaagttcgcc gctacagacg tattaaagat tgcgatgcca gcgttgtcgg
     5101 gaatgtcagt cgtgtaagta gcatcacctg tggccgtggt gccgtttccc gccggatacg
     5161 aagccagcga gtagccaacg gagttaacac agaggtagtg gcctctgttg gggatcacag
     5221 taccgttcgg gattacacag cgagcgacac cgttggaagc agcaacagca taaccagtcc
     5281 cgccacctgc aaccgtgtga tcagcgccac tgttgttgaa aatctcgatg aactcatcat
     5341 tcaagccatt cgggccacgc acgcgaaatt ctgagatgac aagttgtcca acggtggcaa
     5401 cgttcggcgc gggaacatct ttggaaattc tctccgcatc cggggactct ttgactttca
     5461 ctgaactctg gtgaccgttt aatgacttct gcgctctgat agtctgcacc gtgcacaaga
     5521 acgaactgaa cacgagcgcc gctatcaaag gcagcctgta ttgcatggac ttaagcatgt
     5581 ttttctctct cctgcgtttt acgcaaaatc agtcatttga agcggaggtg ccccgagaaa
     5641 ctgttcaatg ttccaatagt tctctggtct cgtggtgatg gatgtgtatg caggcgggtg
     5701 atacaacgaa gtcatcatac gatttgatct tgaggtgcgc aacgcttttt ttggattaac
     5761 attcacaatc ctggaccagg tgggccaagt cagccagata ccctgcaaaa aataagggga
     5821 aggcagtctg ccttcccctt gtgctttccc aaactaccaa tttctgtcac tcgtgatctg
     5881 tgtcttgacg tttctttaac gcgaaactca aaactcaaga cgtttctaag gtagcgcttc
     5941 gacgttgacg aagaagcgga aagtaccccc ctgggccaca cccaggagat atctgacgtt
     6001 gactgacgca cccggtgcca ggaaccctcc gggaatcgcc acaacaacac tcgagttcga
     6061 gccaccgccg gtaactgcgt ctgaaggact ctcaagcgca gtccccctga tggtgattgt
     6121 gcctccatta gcggtcgtat ctactgaggt taggatacgc aagtcggcca gtggccctcc
     6181 cggcgtgttg gtagtggtga tgtcgacagc gcggaaacgc aagcgggtaa tgattgctcc
     6241 cgtgttattc gtgaacttgc gtcgaatatc gagagtacca agtgccgttg ctcccgttcc
     6301 cggcgtcgta tcgcgcacgc ggttcggcgg agcgctcgac gattgggtcg gatcgatcaa
     6361 gccggccttt actgttgcat tgcgttggat tggactcgca agattctcag gtcccggtgc
     6421 gcctcgtact gagagaacac cgaatgtgcc tgccgtagtg gaaacaaact cgaagtctgc
     6481 cgcattgtct cctgtgtctt ttggaaaacc ggtggtcagc ttgcgaacga atgcgtactc
     6541 tccatcaccg gcgatcggag caagccgagt tccttcggta aagagtggag actgtgcccc
     6601 gttctgagac gtaaagttaa ccgtgtcgat tctcgtggcc gcggacaagt tcgcgacgtt
     6661 ggcggtattg aacaatgcca cgccggtgtt gtccgcaatg tcggcagtgt aagtcgcgtt
     6721 aggtgtagcg ttgcctgccc caccatagtt cgacaggctg tagcccaccg atgctgtatt
     6781 gttggcaatt aggtaatgac cgcgcgccgg tatcattgtg ccgtttggta tcgttgcgac
     6841 aattgaggcc actgtgcccg cggcgttcaa tgccgcgact gaccatccgg cggaagcgtc
     6901 gctcgcatta acggtaaaag gcgcaccgct cacatttacg atctcaatgt attcgtcgag
     6961 cgcacccagc gcaccgcgaa gcctgaactc gccaatggca agcgtggttg aagccagggg
     7021 aggcgttcct cccgtaacag taacggtgaa agaacaagcc ggaccggcac cagtggtgac
     7081 gttcacggtt gtcgtaccaa ccgggaaggt agaaccggaa gcgggactgg cactgattgt
     7141 ttggcaagct ccggtgaccg caggattcgg atagttgacg actgcgctcg ttccaccgcc
     7201 atcggccgga acagtaatgt ttgtcggaca cgtgatcgaa cacgcttggg tgttgcaaac
     7261 agcgccgctg gaggtcagcg tcaactccca accaccggcg aaactgccaa tgtccgtgcc
     7321 ggcatcgtcg actacgtaaa gactccatgt accggtggga ctcagaccgt tgaacgcaga
     7381 agcgagcgtg gctgagccgg cggttgccgg atccaaatac ggagccgcag gagcgggcgc
     7441 tggaaaaaga tcacccgtgc cgtagttagt tggttggaaa gtgccggaag cgggcggtga
     7501 acccgatgcc gggaacaagg ccgccgccgc atcatcgatt gtataggtct gccccgtcca
     7561 gtcagttcca ccaatgacat cagacatcag cacaaacttc acgcctgttg gtcccaccag
     7621 caggacatcg acgtctgacg ggaacgtgtg actgatgttc tttagcgtga ctgtcacttt
     7681 ggaaacggga ccagtgaagc cggcgactgt gatgtttgag ggataaggtg tggcaggcga
     7741 acctgtcgtc gcaccagtgc ccgatgccgg aatgactatc gcagtcgcgt tggagaacgt
     7801 ttgcgtactg ttggtgctcg tacctaatgt gaactggaac gtcaccgtgc ccaaatccac
     7861 gatgccatcc tggaaatgaa gcgttgcggt gactgtgctg ccgcaagctc cggcaacgct
     7921 gaaggtgtac ggctgcgaaa ccgggggact gccaggagtg atgacgccat aggttctatt
     7981 cggattgcca accggagtga tgccacccga ggtttgcaga gttcccacca ggcccgcagt
     8041 tgttg
//
LOCUS       DASUEX010000271         2040 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1177391, whole genome shotgun sequence.
ACCESSION   DASUEX010000271 DASUEX010000000
VERSION     DASUEX010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1177391"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1886)
                     /locus_tag="VFV61_04585"
     CDS             complement(<1..1886)
                     /locus_tag="VFV61_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899785.1"
                     /translation="MIISLVLTLLVTASGTIATYLYDEGASFATRVCTGACIGITALG
                     LLGFVVASFLGLTSTSILITAALLTLPFLILKDTRRRKLVRGDGKATWQALKRNLFNP
                     SGRNLGYALFYAAIAAILWRVFDRAMIESPEGLATGVLNNFGDLPFHISVITSFAFGN
                     NFPPEDPTYAGVHFTYPFLTDFVSAIFVRCGASLRQSMFLENFVLAFSLVVLVHRWAL
                     ELLKDRLAAIVTPVLVLLNGGCGWLLLWTEMKRSDAGLLGALKALPPSVTVIPDTTWR
                     WGNAVSTLLVPQRGMLLGLALAVIVFTQWWLSEEKVEEGNRVSGKSKKAQKKNVPTSS
                     PTPVPLFPFYPFLLSQRRMIAAGLVAGALPLVHAHSFMVVLAVGAGIALCQRRWRDWI
                     VFFSFASLLALPQVWWSTHNSAVDARTFFDWQFGWDRGTENAVWFWLKNTGIFIPLIV
                     LAILWRGKNYLISRRLLVYYLPFTLCFIVPNFIKLAPWVWDNIKVLYYWWLASAPLVA
                     LLLARLWQQRGVKRLVAFTLFVLVILAGSLDVASILTRSTKFGVFDPQGIAFAEMIKK
                     QTDPRSLIVHAPVHNHPAFLAGRRSLLGYPGHIWTHGLEFAPRENEIKRIYSGSSDAE
                     ALLR"
BASE COUNT          551 a          540 c          561 g          388 t
ORIGIN      
        1 tttcttagca gcgcttctgc gtcggaactc ccggagtaaa tccgtttgat ttcattttcg
       61 cgcggagcga attcgagtcc gtgagtccag atgtgaccgg gataacccag caacgaacgt
      121 cgtcctgcaa gaaatgccgg atgattatgc acgggcgcgt gaacaatcaa actccgtggg
      181 tccgtctgct ttttgatcat ctcggcgaag gcaattcctt gagggtcgaa tacgccgaac
      241 tttgtcgatc tggtcaaaat ggaagcgaca tccagactgc cggccagaat caccaggaca
      301 aaaagtgtaa atgcgaccag acgcttgact cctctttgct gccaaagccg cgctaagagg
      361 agggctacca gcggcgcgga agcgagccac cagtaataaa gcaccttgat attgtcccat
      421 acccagggag ctagtttgat gaaattggga acgatgaaac aaagcgtgaa gggaaggtag
      481 taaaccagca gtcgccggga aatcagatag tttttgccac gccagagaat cgccaatacg
      541 atcagcggaa tgaagatgcc ggtgttcttc agccaaaacc agactgcgtt ttcagtgccg
      601 cggtcccagc caaactgcca gtcaaaaaac gtcctggcat ccaccgcgct gttatgagtt
      661 gaccaccaca cttgcggcaa tgccagcaat gaagcaaaag aaaagaatac gatccagtca
      721 cgccaacgcc gttggcacag agcaatacct gccccaaccg ccaatacgac cataaaacta
      781 tgggcatgaa cgaggggcaa tgcgcccgcg accagaccgg cagcgatcat gcgccgttgc
      841 gacaggagga aagggtaaaa gggaaacagg ggaactggcg ttggactcga cgttggcaca
      901 ttctttttct gcgccttctt gcttttccca ctgaccctgt tcccctcttc cactttttct
      961 tcagacagcc accactgcgt gaacactatc accgcgagcg ccaagcctaa gagcatgccc
     1021 ctttgaggca ccaacaaagt ggaaacagca ttgccccaac gccaggttgt gtctggtatt
     1081 acggtgacag acggcggcag agctttcaag gcgccgagca atccggcgtc gcttcgcttc
     1141 atctctgtcc agagcaatag ccagccgcag ccgccgttca gcagtaccaa cacgggtgtc
     1201 acgattgccg ctaagcggtc tttgagtaac tccagcgccc aacggtgaac taaaacaact
     1261 aaggagaaag ccaggacgaa attctccaaa aacatggact gccgcaagct cgcgccacaa
     1321 cgcacaaaaa ttgcggagac aaaatccgtc aggaaagggt aagtgaaatg cacccccgcg
     1381 tatgtcggat cttcgggagg gaagttgttg ccgaaggcga aactggtgat gacgctgatg
     1441 tggaatggca ggtcaccaaa gttattaagg acaccggtag ccaagccctc gggtgattca
     1501 atcattgcgc ggtcaaagac gcgccagagg attgcagcaa tggcggcata gaaaagagcg
     1561 taccccaggt tcctgccact tgggttgaac aggttgcgct ttaaggcctg ccaggttgct
     1621 ttcccatcac cccgaacaag cttgcggcgc cgagtgtctt tgaggattag aaaaggaagg
     1681 gttagcagcg cagcagtgat caagattgag gtgctggtaa ggcccaggaa ggacgcaacg
     1741 acaaaaccga ggaggccgag agctgtaatt ccaatgcagg cgccggtgca cacgcgcgtt
     1801 gcgaaggagg caccttcgtc atagaggtag gtggcaatcg tgccgctcgc ggtaacgagc
     1861 aaagtaagca cgagcgaaat gatcatgagc tagagattat acgcgatgcg ccttgcgatt
     1921 gccgattgcc gattgccgat tggccggtac agtacgggag cggtagcgac cgcattcaac
     1981 taccgaaagg ctcgacattc ggacaatcag atcgaacaag ttctaaagag ctgcaatcgg
//
LOCUS       DASUEX010000272         2015 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_971856, whole genome shotgun sequence.
ACCESSION   DASUEX010000272 DASUEX010000000
VERSION     DASUEX010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_971856"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(103..1026)
                     /locus_tag="VFV61_04590"
     CDS             complement(103..1026)
                     /locus_tag="VFV61_04590"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HEX4899786.1"
                     /translation="MNSGVAESIIDLVGHTPLLHLARFAPPPLAGIYAKLEWFSPGGS
                     VKDRAALGMILAAERQGKLKPGTTIIEPTAGNTGIGLALIGVARGYRVILCVPEGFAK
                     EKMKLMSALGGEIEYVSRESGITGAINRAQELAATIPNSYIPQQFENQANPDFHEATT
                     AAEIIEQLAGDIDAVVIGAGTSGTFVGVSRAVRKLNPNVLCVLVEPEGSIYGGKPPGP
                     KKIEGIGQTQFLPEIYDASLADEVLMVMNDEVCATFRRLARTEGVLGGWSSAAAAAGA
                     YRIAERLGAGRKVVTVFPDGAERYISQGIFD"
     gene            complement(1123..1938)
                     /locus_tag="VFV61_04595"
     CDS             complement(1123..1938)
                     /locus_tag="VFV61_04595"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX4899787.1"
                     /translation="MTRLSIVGIIIVALLVIVALFAPWIATHEVGATDLSLRYLAPSA
                     AHYFGTDSTGRDIFSRVVFGARISLQVGIIVVGVSAVIGTLLGALAGYYGGWIDRFIS
                     GYVFNVFLAFPGLLLAIAMVAFLGAGLNKLILALCIIGWVGYARLIRGQVLKVREYDF
                     VQAARALGASDARILLIHILPNAIQPLIVQASLGMAGAVLAEASLSFLGLGVPPPAPS
                     WGVMIEEARDLSTLQAAPHALIFPGIVIALTVLAFNFIGDGLREYLDPRQRAR"
BASE COUNT          475 a          565 c          567 g          408 t
ORIGIN      
        1 agttggattg ttaggcttaa gccgaatgcc attccaattg ccgattgctg atcgctgatt
       61 gccgagtgtg attgcaaatc cgcaatccgc aatcgccagt tctcaatcga atattccctg
      121 gctgatgtaa cgttcagcgc cgtcgggaaa gacggttaca acttttcttc ccgcaccgag
      181 tcgttctgcg atgcggtaag cgccggcggc cgcggccgcc gatgaccacc ctcccagaac
      241 gccttctgtt ctcgccagcc ggcgaaatgt cgcgcaaacc tcgtcgttca ttaccatcag
      301 cacctcgtct gcaaggcttg cgtcataaat ctccggaaga aactgcgttt gcccaattcc
      361 ttcaatcttt tttggtccgg ggggcttacc accatagatc gaaccctcag gttcgacaag
      421 cacgcacaaa acgttcgggt tgagttttcg cacggcacgt gaaaccccga caaacgtccc
      481 cgatgtgccg gcgccaatga cgactgcgtc aatatcaccc gcaagctgtt cgatgatctc
      541 ggcggcagtt gtggcctcgt gaaaatccgg attagcctga ttctcaaatt gctgcggtat
      601 gtaactgttc gggatcgtcg cagccagttc ctgggctcgg ttgatggcgc cggtgatgcc
      661 cgattcccgc gagacatatt cgatttcacc accaagggcc gacatcaatt tcatcttctc
      721 tttagcgaac ccctcaggaa cgcagaggat aacacggtag ccacgcgcga cgccgatcag
      781 cgccagaccg attccggtat taccagctgt tggctcaatg attgttgtgc ccggcttcaa
      841 tttgccctgc cgttccgcgg cgagaatcat tcccagcgcc gcgcgatctt tgacggaacc
      901 accaggacta aaccactcga gcttggcata aatcccggct aaaggtggcg gtgcgaaacg
      961 agcaagatga agtaacggcg tgtggccaac gaggtcgatg atgctctcag ctacgccgga
     1021 attcatgaag tcaggtgggt tggaaatgcg atcgagttgt attgcgctgt tgcaaggcga
     1081 tcgtagaccg cgtgtttttg agtgtcaaac gacgacctga aattaacgag cgcgttgccg
     1141 cggatccagg tattcacgca acccgtctcc aatgaagtta aaagcgagaa ccgtgagggc
     1201 gataacgata cccggaaaga tgagagcatg aggggcggct tgcaaagtcg aaagatcgcg
     1261 agcttcttcg atcattacac cccaggaagg agcgggcggc ggtaccccaa gcccgagaaa
     1321 cgagagtgaa gcttcggcga gaacggcgcc tgccatgccg aggctggctt gcacgatcag
     1381 cggttggatc gcatttggaa ggatgtgaat cagcaaaatg cgggcgtcgc tggcgccgag
     1441 agcgcgcgcc gcctgcacga agtcatactc gcggactttt aacacctgac cgcgaatcag
     1501 gcgtgcatag cccacccagc caatgatgca aagagcgagg atcagcttgt tcaagccggc
     1561 tccgaggaat gccaccatgg cgatagccag tagcaacccg gggaaggcga ggaacacgtt
     1621 gaagacgtaa ccggagataa agcgatcgat ccagccgccg taatacccgg ccagagcgcc
     1681 caagagcgtt ccgatcaccg ctgagacccc gaccacgatg attcccactt gcagggagat
     1741 gcgagcgcca aaaaccacac gcgagaagat gtcccgcccg gtagagtcag tgccgaaata
     1801 gtgggccgcc gacggggcaa ggtagcggag gctgaggtcg gtggcgccca cctcgtgtgt
     1861 agcgatccac ggcgcaaaca gcgccacaat cacgaggagc gctacgatga tgatgccgac
     1921 tatgctgaga cgagtcattt ttgaactttg aacttggtcc tttgaactta gtacttcgag
     1981 cttggtacta gttgctgctt atgactcaat gcaac
//
LOCUS       DASUEX010000273         7374 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1086669, whole genome shotgun sequence.
ACCESSION   DASUEX010000273 DASUEX010000000
VERSION     DASUEX010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7374
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1086669"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /locus_tag="VFV61_04600"
     CDS             <1..513
                     /locus_tag="VFV61_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899788.1"
                     /translation="LRELAAAETAKPNSAAPVVYCSDLRLADALPNTAPQPILWASHL
                     FVFSGSSALENRERLYQQLYYSGVNEQEFAAQAGRDSFLQLALFGWERMMQQAHTRAI
                     GEEDVRRETSLYAEYIGAFNAARAAAPPVGYLVTPAMGGPSLTNFDRWYERGSGERIG
                     DYMLYRPRQR"
     gene            complement(593..4723)
                     /locus_tag="VFV61_04605"
     CDS             complement(593..4723)
                     /locus_tag="VFV61_04605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899789.1"
                     /translation="MSTLPLSRERRATTRRQIIALTLVTLLVLSLPGSSLLRLSSFSV
                     ALADASLQTLPFSQNWANNGLITTNDIWTGVPGVQGFLGQDLTTATGTDPQTILTGAS
                     GLAGDLTVLANQTSTAVTNGDVGEFHTTSQTGGPATDSTIALQGSGTADAPHIILYLN
                     TTGQTGINVSYNLRDIDCTTDNSIQQVALQYRVGNSGNFTNLPAGYVADASAGPSLCT
                     QVTPVSVALPVAAENQAEVQVRIMSTNAVGNDEWIGVDDISVGTGPTLPTLNINDVAL
                     AEGNAGTTTFTFQVSLTASGPSVVTFDIATADGTAQDDNPATEDNDYVANSEIGRTIT
                     SGNTSATFSVTVNGDTTTEPNETFFVNITNVTGANAGDVQGLGTINNDDVTLTAIHAI
                     QGTGSTSPLAAQVVTTSGIVTGLRTNGFFVQEPDATVDADPNTSEGIFVFTSSAPPAA
                     AALGNSVQVTGTVAEFIPSADPSSPPLTELTSPTVLQLSTGNPLPTPVVITAGETTLP
                     SETSNPLDTLEEFEGMRVTVASLTAIAPTQGNITESSATANSNGVFYGVVTGVSRPFR
                     EPGISISDPLPTGAPGSIPRFDENPERLRVDSDGQVGATAINVTTGTVVTGITGVIDF
                     GFRTYTILPDAATPPSVGSISTFTPVPNQTANELTVGSFNMQRFYNDVDDTGGDVVLT
                     TTAFNNRLNKASLAIRNAMRSPDVIGIVEMENLATLQAVASKVNSDAVANMEPNPNYQ
                     AYLVEGNDIGLIDVGFLVKAARVSVVDVTQFGLTDTYINPNTGSPEILNDRPPLVLRA
                     TIAKPAGGTLAFTVIVNHLRSLNGVDDPTPSGSGTEGARVRAKRRAQAEYLANLIQAR
                     QTNDPTEKIISVGDYNAFQFNDGYVDSMGTITGVPTAAAEVVLASSDLVNPDLTNLIS
                     TVPTDQRYSFTFDGNAQSLDHVLVNDDALAILNRFAYARNNADFPQVYFGDANRPERI
                     SDHDMPVAYFSLGGIPVGSVVISEFRFRGPSVVPGQVDGSLDEYIELYNNTDAAITVN
                     SVDGTSGWALSYLDSSGALQNIAAVIPNGTVIPARGHYLLTNEPPLAPPGPNGITPVG
                     EYTLDSYANADQRYRAGAGLINISDDSAIAVFQTANISNFSLATRLDAVSLSSVAGAT
                     ADLYREGTNLPSPGANDGQYAYVRKLVTGLPQDTNNNAADFVFVSTNGGSYGGVQSQL
                     GAPGPENTLSPIQRNSTIKPGLIEPQNSSSAAPNRVRDLTPVTNGALGTLSVRRRFTN
                     QTGAPITRLRFRVVDITTLNSPGYAPGGSQADVRVIDGSDFSITTSLGMLTVRGLMIE
                     QPAPLVQPNGGGLNSSVTVTIPGGAIANGASIDVHFLLGVQQGGTFRAFFNVEGLP"
     gene            complement(4996..5265)
                     /locus_tag="VFV61_04610"
     CDS             complement(4996..5265)
                     /locus_tag="VFV61_04610"
                     /inference="COORDINATES: protein motif:HMM:NF017239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HEX4899790.1"
                     /translation="MNTSATPHPHSHVVYTEFDGREAVLVDLNTKRYYTLNETAMLVW
                     RGLERGQTTTEIAREICDRYEVSEERAAASIERLLTSLGAHRLLS"
     gene            complement(5286..6380)
                     /locus_tag="VFV61_04615"
     CDS             complement(5286..6380)
                     /locus_tag="VFV61_04615"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX4899791.1"
                     /translation="MTVTTEVPQRQDPVRLTVELTNICNLHCSYCMRDEDALYHTKAN
                     YFPAELLGRIVREARDSYQIDYVTFTGGEVTLHPRFNDMIAAVASEGMQCSFVTNGWH
                     FDRVYPAIMANKQAVRLVAFSVDGATREAHDFWRGAGSFDRVIRAVTRCHMQNIPFVF
                     KVGIRRDTMTELERIALLAARLGAAALHFSHLLPTSAEAENESALSLEEQRHAEQEVM
                     GLAKILKMPIGIAAGYYDIHSEVPCSPLKTTSCNVDWRGRLTLCCNLAGYRNADGEPD
                     VLADLNRESFAVGYARLKALAQQQLERRRQALAEIAARGETPDLYTGSPCLFCLQSFG
                     KIPWHNSQPNSSAASGRSLPVIVAPATTSD"
     gene            complement(6390..>7374)
                     /locus_tag="VFV61_04620"
     CDS             complement(6390..>7374)
                     /locus_tag="VFV61_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899792.1"
                     /translation="ISHYNIAGRLLRVHTLGDWETQWVAGFLSGFHLEKEFAPTDQPS
                     ITLTVRRAPLPNVPSGLQSFAINRGHCYTDESQYFLTVDESLVHICTPAERQVEVWLG
                     VTEKARHPVALVNAFSYGLQAALRRAGVFDVHAAAAVEPRSQAGALFVGASGSGKTTL
                     TLRLTESGWLYLSDDMVVLRETRTALEAEGLRRLFAVADTSVIGSSSTRLNAALGAPV
                     ASDPSKRRLEPETVFPESRAASCQPRALFFPQVVAAAETRINQIGSRAAMEQLLRHCP
                     WATYDTCTAPEYLRVLARLANQCRAYELAAGRDLLLHPERAADLLAPYMTE"
BASE COUNT         1637 a         2011 c         2109 g         1617 t
ORIGIN      
        1 ttgcgcgaat tggcagcagc agaaacagca aagcccaatt ccgctgcgcc ggtggtgtac
       61 tgcagcgacc tgcggttagc ggatgcattg ccaaacacgg cgccccagcc cattctctgg
      121 gcgtcgcatc tgttcgtttt ctcaggctcg tccgctttgg aaaatcgcga gcggctttac
      181 cagcaactct attattcggg tgttaatgaa caggaatttg cagcgcaggc cggcagagat
      241 agtttcctgc agcttgcgct cttcggttgg gagcgcatga tgcagcaggc gcatacgcgg
      301 gccattgggg aagaagatgt gcggcgagaa acctcgcttt atgctgaata cattggggcc
      361 ttcaatgcag cgcgcgccgc ggcgccccca gttggttatt tagttacgcc ggcaatgggt
      421 ggaccctcac tgacaaactt tgatcgttgg tatgagcgag gttcggggga gcggattggg
      481 gactacatgc tttatagacc acggcagagg taatcggcag taggcttggc gcagagcaag
      541 gcaaggatat ttatcatttc tcatccttgc ccagccgtct gcctcctgct ttctaaggta
      601 aaccttccac gttgaagaac gcccggaagg tgccgccctg ctgcacacca agcaggaagt
      661 gcacatcgat tgatgcgccg ttagcgatgg ctcctcccgg aatcgtcacc gtgacagatg
      721 aattcaggcc accaccattc ggctgcacca gtggcgctgg ttgttcgatc atcaatccac
      781 gaactgtcaa catgcccagg ctcgtggtaa tgctgaaatc agagccatca atgacccgca
      841 cgtctgcctg cgatccgccg ggcgcgtagc cagggctatt cagagtcgtg atgtctacca
      901 cgcggaagcg aagacgcgtg attggggctc cggtttgatt tgtgaagcga cgccgcaccg
      961 acagtgtgcc cagcgcacca ttggttaccg gtgttagatc acgcacccgg ttaggggcag
     1021 cgctggagga attttgcggc tcgatcaagc ccggtttgat cgtcgagttc ctttgaatcg
     1081 ggctcagcgt gttctccggt ccaggcgcgc ccagttgcga ctgcacgcct ccgtagctac
     1141 ccccgtttgt cgaaacgaaa acaaagtccg ccgcattgtt gttcgtatcc tgcggcaaac
     1201 ctgttaccag ttttcgcacg tacgcatact ggccgtcgtt cgctccaggc gacgggaggt
     1261 ttgtaccctc gcgatagaga tcggcagtcg ctccggctac ggaactcagg ctaaccgcgt
     1321 cgagtcgcgt ggcaagagag aagttggaga tattagcggt ctggaacaca gcgattgcag
     1381 aatcgtcgga aatatttatc aagcctgcgc ctgcacggta tctctgatcg gcgttagcgt
     1441 aactatcaag cgtatattca ccgaccggcg tgattccatt tggtcctggc ggcgcaagcg
     1501 gcggctcatt cgttagcagg taatggccgc gagcgggaat gaccgtgccg ttgggaatta
     1561 ctgccgcgat gttttgcaaa gctccggaac tgtcgagata cgacaaggcc cagccgctgg
     1621 taccatcaac agaattcacg gtgatggcgg catcggtgtt gttgtacagc tcgatgtact
     1681 catcaagtga gccatccacc tggccgggaa caactgaagg accccggaag cgaaattcgc
     1741 taatgactac cgaacccacg gggatacctc ccaatgagaa ataagctacc ggcatgtcgt
     1801 gatccgagat ccgttcaggc cggttggcgt cgccgaaata gacttgcggg aagtctgcgt
     1861 tgttccgcgc ataagcgaag cgattaagaa ttgctaacgc gtcatcgttg accagcacgt
     1921 gatcgagcga ctgcgcatta ccatcgaaag taaacgagta gcgctgatca gtaggaactg
     1981 tgcttatcaa attggtgaga tctggattaa ccaagtcact tgaggcgagt acaacttcag
     2041 ccgccgccgt gggaacaccg gtgatggtac ccatgctgtc gacataaccg tcattaaact
     2101 ggaaagcgtt gtagtcgccg acggaaatga tcttctcagt aggatcgttt gtctggcgcg
     2161 cctgaatcag attcgccaga tactcagctt gcgcgcgacg tttggcgcgc acgcgcgcac
     2221 cttccgtacc tgaaccgctg ggtgtgggat cgtcaactcc attcaacgag cgcaagtgat
     2281 tcacaatcac agtaaaggcc agcgtgcctc cggccggctt ggcgatcgtg gcgcgcaaaa
     2341 ccagaggcgg ccggtcgttt aagatctcag gtgagcctgt attgggattg atataagtgt
     2401 cagtcaggcc aaactgcgtg acatcaacga ctgaaacgcg cgcagccttc accagaaaac
     2461 caacgtcaat caaaccgata tcattccctt caacgagata ggcctgatag ttaggattgg
     2521 gctccatatt ggctacggca tcgctgttaa ccttgcttgc caccgcctga agtgtcgcta
     2581 agttctccat ctccacaatg ccaatcacat ctgggctgcg cattgcgtta cgaatcgcca
     2641 gcgatgcttt gtttagtcga ttgttgaaag ctgtggtagt taagaccaca tcgccaccgg
     2701 tatcgtcgac gtcgttgtag aagcgctgca tgttgaaaga gccgactgtt agttcgttgg
     2761 cggtttgatt cggaacgggc gtgaaggtcg aaattgaacc tacgctcggc ggcgtggccg
     2821 cgtccggcag aatcgtgtag gtacggaaac caaagtcgat tacaccagtg atgccggtga
     2881 caactgttcc ggtggtcaca ttgatcgctg tcgctccgac ctggccgtca ctatcgactc
     2941 gcagtcgttc agggttttca tcgaagcgcg gaatacttcc cggcgcgccc gtcggaagcg
     3001 gatcagaaat gctaatcccc ggctcacgga agggccggga gacgcccgtt actacgccat
     3061 agaaaacgcc gttggagttc gctgtagcgc tcgactcggt gatatttccc tgagtgggcg
     3121 caattgcggt cagcgacgcc acagtcaccc gcatgccttc gaactcctcc aatgtgtcga
     3181 gcggattgga ggtttcggac ggcagcgtgg tctcgccggc agtaattacc accggagtcg
     3241 gcagcggatt gccggtcgaa agttgcaaga cggttggact ggtaagttct gtcaggggtg
     3301 ggctgctcgg atcagcggag ggaataaact cagccaccgt tcccgtgact tgcacgctat
     3361 tgccaagcgc ggcggccgcg gggggagcag acgaggtgaa aacaaagatg ccttccgatg
     3421 tgttgggatc ggcatcaaca gtcgcatcag gctcctgaac aaaaaagccg ttagtcctta
     3481 agccggtgac gataccggac gtcgttacta cttgtgccgc gagtggcgaa gtggaaccgg
     3541 taccttggat cgcatgaatc gccgtcagcg tcacatcgtc attattgatg gttcccaggc
     3601 cctgcacgtc acccgcgtta gcaccggtaa cgtttgtaat attgacgaag aaggtttcgt
     3661 tgggttcggt cgtggtgtcg ccgttgactg ttactgagaa agtggccgag gtatttccgg
     3721 aagtaattgt tcgtccaatt tccgagttgg ccacatagtc gttatcttcc gttgccggat
     3781 tatcgtcctg ggccgtgcca tccgcggtgg cgatgtcaaa agtaacgacg ctcggcccgg
     3841 acgcagtcaa acttacttga aaggtaaaag tggtggtgcc agcattgcct tcagccaacg
     3901 ccacgtcgtt gatgttcagc gtcggtaggg tgggccccgt gccaacactg atgtcatcca
     3961 caccaatcca ctcatcattt ccgacggcat ttgtcgacat gatgcgcact tgaacttctg
     4021 cctgattctc tgctgctacg ggcagagcga cgctcactgg cgtcacctga gtacagagac
     4081 tgggccctgc tgacgcatct gcaacataac cagccggaag gttcgtaaag tttccgctat
     4141 tgccgacacg atactgcaat gcgacctgct gtatagaatt gtccgtagtg cagtcgatat
     4201 cgcgcagatt gtaggagacg ttaattccag tctgacccgt agtgttcaaa tagaggatga
     4261 tatgcggggc atctgccgtg cccgatccct gaagtgcaat cgttgaatcc gtagcggggc
     4321 caccggtctg cgaagtcgtg tgaaactcgc ccacatcacc gtttgtgacc gccgttgagg
     4381 tttgattagc aagaacggtt aggtcgccgg caaggccgct ggcccccgtc agaatggtct
     4441 gcggatcggt accggttgcc gtcgtcaaat cctgtcccag aaaaccctgc actcccggca
     4501 cacccgtcca aatatcattg gtggttatca gaccattgtt tgcccagttc tggctgaagg
     4561 gcagcgtttg caaggaggca tccgcaagag ctaccgagaa cgacgacagt cttaacaatg
     4621 agcttccggg aagcgacagc accaataacg tgaccaaagt caaagcaata atctgacggc
     4681 gcgtggtagc gcgtctttca cggcttaaag gcaatgtaga cacagtgtga ttcctttcgg
     4741 gttgttacta cggttttggt agggtaagac gcaacctgga attgcgtcgt cccgtgcaga
     4801 tgccaattaa gaaacgaaag attgcgatcg acgctccgct atcgggcaga ccacggggag
     4861 attgctactg aataggagtg gcggtcggca cggcaacctt atatcacagt gtgcaggcgg
     4921 cagatacatg aagcacacaa agtcacgatt tgatcacgag tgggattatc cgcactgcgc
     4981 gagcggctaa ttgaattacg acaggaggcg atgcgccccc agcgaagtca gcagtcgctc
     5041 gatcgaggct gcggcacgtt cttcacttac ttcgtagcga tcgcaaatct cgcgcgcgat
     5101 ctcggtcgta gtctgaccac gttccaagcc gcgccacacg agcatcgccg tctcgttcag
     5161 tgtgtaataa cgcttggtgt tcagatcgac gagcacggct tcgcgaccgt cgaattcggt
     5221 atagacaaca tgactgtgtg ggtgtggtgt agccgaagtg ttcaagggtt ctctttcctg
     5281 gtgaatcagt cgctggtagt ggctggcgcc acaattaccg gcagcgaccg gccgctggct
     5341 gcggaactgt taggttgcga attgtgccag ggaatctttc caaagctttg caggcagaag
     5401 agacagggtg atccggtgta aaggtcaggc gtctcgcccc gtgctgcaat ttcggccagc
     5461 gcttgccggc gtcgttcgag ttgctgctgc gccagggctt tcaaacgcgc atagcccacg
     5521 gcaaaactct cgcgattaag atctgccagg acatcaggct cgccgtcagc gttccgataa
     5581 cctgcaagat tgcagcaaag cgttaaccga ccgcgccagt cgacattgca actagtagtt
     5641 tttaacggtg aacagggaac ttcggaatgg atgtcgtaat aacccgcggc gataccgatg
     5701 ggcatcttta gaatcttcgc gagtcccatc acttcttgtt cggcgtgacg ctgttcttca
     5761 agcgataagg ctgactcgtt ctcagcttcc gctgaggttg gcaataaatg cgaaaagtgc
     5821 agcgcggcag caccgaggcg ggcggccagt aaagcaatcc gttccaactc ggtcatcgta
     5881 tctcgccgga taccgacttt gaacacgaac ggaatgttct gcatgtgaca gcgcgttacc
     5941 gcacgaatta cccgatcgaa cgatccggcg ccgcgccaga agtcatgcgc ttcgcgcgtg
     6001 gctccgtcta cgctgaatgc cacaaggcga acggcctgtt tgttagccat gatcgccgga
     6061 taaactcgat caaagtgcca accgttagtg acgaagctgc attgcatgcc ttcgctagcc
     6121 actgcggcaa tcatgtcgtt gaagcgtgga tggagcgtga cttctccccc cgtaaaggtt
     6181 acgtaatcga tctggtatga gtcgcgcgcc tcgcgcacga tccgccccag cagctccgcg
     6241 ggaaagtagt tagctttggt gtgatagagc gcgtcttcat cgcgcatgca atagctgcaa
     6301 tggagattgc agatattcgt cagttcgaca gttaatcgaa ctggatcctg gcgctgagga
     6361 acttcggtcg tcacggtcac gaaacctttt tactccgtca tgtagggtgc gagcaagtca
     6421 gcggctcgct ccggatgaag caacaagtca cgacctgccg ctagttcata agcacgacac
     6481 tgattcgcca gccgcgccag cacccgcaag tactcgggcg cggtacaagt gtcgtaagta
     6541 gcccagggac aatgtcgcag taattgctcc atcgcggcgc gcgatccaat ctgattaatc
     6601 cgcgtctctg ccgccgccac cacctgggga aagaaaagcg cccgcggttg acagctcgct
     6661 gctcggctct ccgggaaaac agtttccggt tcgagtcgtc gtttcgaggg atcggaagcc
     6721 actggcgcac ccagggcggc gtttaatctt gtactactgc taccaattac agaggtgtcc
     6781 gccacggcaa acaaccggcg cagtccttcc gcctccagcg ctgtgcgagt ctcgcgcaac
     6841 accaccatgt catccgacaa gtaaagccag ccggactccg tgagacgcag cgttaatgtt
     6901 gtcttgccgc ttccggatgc acccacgaag agcgcgccgg cttgcgaacg gggctcaacg
     6961 gctgcggcgg catgcacatc gaaaacgccg gcccggcgca acgcggcttg cagaccgtag
     7021 gaaaaagcat tcaccagtgc gactggatgt cgcgcctttt cggttacgcc aagccaaacc
     7081 tcaacctgtc tctccgctgg ggtacagatg tgaaccagag attcatctac agtcaaaaag
     7141 tattggctct catccgtgta gcagtggccg cgattgatgg caaacgattg caaccccgaa
     7201 ggaacgttgg ggaggggcgc gcgtcttacc gtaagtgtta tcgacggctg gtcggtgggc
     7261 gcgaactctt tttcgaggtg gaagccgctt aagaaaccgg caacccactg cgtttcccaa
     7321 tcgccaaggg tgtgaacgcg cagaagtcga ccggcaatat tgtaatgact aatc
//
LOCUS       DASUEX010000274         3394 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_732549, whole genome shotgun sequence.
ACCESSION   DASUEX010000274 DASUEX010000000
VERSION     DASUEX010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3394
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_732549"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="VFV61_04625"
     CDS             complement(<1..410)
                     /locus_tag="VFV61_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899793.1"
                     /translation="MGRVFDALRRASSTTEGEAAKPENDSAQQRSKVRAERALPSAKE
                     VEEQLFSGSSILHVAEDPVQSSASTAHTADAPDGSALPGGMASRAAGATLGAAGATRA
                     TGFVAYDISPARVEPHLVAVTQPRSPYCEQYRSLR"
     gene            complement(469..1425)
                     /locus_tag="VFV61_04630"
     CDS             complement(469..1425)
                     /locus_tag="VFV61_04630"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX4899794.1"
                     /translation="MYAEFYGLKELPFALTPDPRFIYFTPSHTEVMANLHYGIESGKG
                     LVVVTGEVGTGKTTILRWMMQRLDRTVLVAYIFNPRLSVSEFYQHVAALLDVQKWETK
                     SELLLELGKALQSRHSRGLRTVLIIDEAQGLSPHVLEEIRLLSNFESDTAKQLQIVLT
                     GQPELREVLNNPDLRQLKQRVALRCVIKPLPNVEETDRYITSRLLVAGAERIDIFSPG
                     ALDYIFRCSDGIPRNINNLCDNALLAGYAAGETTISRAVIEEVAETFDMLPRSDRGLP
                     SGEEREAPARIFSASGRAELWAAGEGSESEPEPVTPATVKGS"
     gene            complement(1476..3104)
                     /locus_tag="VFV61_04635"
     CDS             complement(1476..3104)
                     /locus_tag="VFV61_04635"
                     /inference="COORDINATES: protein motif:HMM:NF014732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNVR domain-containing protein"
                     /protein_id="HEX4899795.1"
                     /translation="MSVEFRQRKPGEYAKIIWKRKWLIILPIIAITTAVSWVVYRLPD
                     VYESTTLIVVKPSTLPNTVVPSITEDSLTRQLTSIAQVVASRSSLEPLVSRYDLYKTE
                     RLRGEPMESIIDAMRRDIKVEVNTSRNDITNGFNITYRYRDPQTTQMVTRELASKYID
                     EQTKNTINSTTSAKNFIEQQARQAKEELDGIDKQRLDFMSRNLGNLPAEGQSLLSQLN
                     GLRDQQKAYITEVGRLQDRRSAMASQLGSIKKSVEQVKDDIAESTTDPKTTPAWGQLA
                     TRKADLEAQLTRMRTELRDKHPDVIAKQAEIDSVQHSMDDMIADWKQKIREKQEKLKD
                     RPDLQVSALENELKLIDSEIKRQQGLIGQIETQIGLVMQRINMVPGAEVELSRLDREY
                     QTKKAAYDSLLVQQQRISLGADAASQQQGETIEVIDAANLPSKPVAPKRFMLSVMGLG
                     IGLVFGFLLVGVMEIPKLLTIQTSEDASHYTNLPVLVSVPELLTPQEARSVPRRRRLL
                     LAAGIAVTIASIPLLALALRATHVFEMFMSTGRA"
     gene            complement(3159..>3394)
                     /locus_tag="VFV61_04640"
     CDS             complement(3159..>3394)
                     /locus_tag="VFV61_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899796.1"
                     /translation="LHSAAVDQLQKAVSIDESIAKNANAPSSPVYRFHLGMALKAKGD
                     KDAARRELETALRLGEKVPFKESDEARKALSTM"
BASE COUNT          707 a          946 c          890 g          851 t
ORIGIN      
        1 cgcaaggaac gatactgttc acagtaaggt gaccgtggtt gtgtcaccgc caccagatga
       61 ggttcgacgc gcgcgggaga aatgtcatat gcgacaaatc ccgtcgcgcg cgtcgccccg
      121 gcagcgccca aagttgcccc agcagcacgc gaagccattc cgccggggag tgccgacccg
      181 tcgggagcat ccgcagtgtg cgcagtggac gctgaacttt gcaccgggtc ttctgcgacg
      241 tgcaaaatcg aagaaccact aaatagttgc tcttcaactt ccttcgcaga aggcagtgct
      301 cgctcagcac gaactttgct tcgttgctga gctgaatcat tttctggttt tgccgcttca
      361 ccttcagtgg tggaactggc tcgtctgagt gcatcaaaga ctctacccat tagtactcct
      421 tctcagtcag ctcaactgat tgctcgagct ggaagagatt caaaccccct agctaccttt
      481 gacggttgcc ggcgttactg gttctggttc ggattcggaa ccctcgccag cggcccaaag
      541 ttcagctctt ccagacgctg agaaaattcg cgccggtgct tctctctctt caccggacgg
      601 taagccacga tcagagcgtg gcagcatgtc aaaggtttca gccacttcct ctatgacggc
      661 gcgactgatt gttgtttcac cagccgcata acccgccagt aatgcgttat cgcagagatt
      721 gtttatgttg cgcggaatgc cgtcagaaca gcgaaagatg tagtcaagtg cgcctggcga
      781 aaagatgtct atgcgctctg ccccagccac cagcaatcgc gaagtgatgt aacgatctgt
      841 ctcctcgaca ttgggcagcg gtttaatcac acagcgcagt gcaactcgct gcttcagttg
      901 ccgtagatct gggttattga gcacttcgcg gagttccggt tgtccagtca aaacgatttg
      961 caactgcttg gcagtgtctg attcgaaatt agagagcagg cgaatctctt ccagcacgtg
     1021 cggggataat ccctgtgcct cgtcgatgat cagaacagtg cgcagtccac gcgagtgacg
     1081 agactgaagc gctttaccca gttcgagcag caattcggat ttggtttccc acttctgcac
     1141 atcaagcaga gcggccacat gctggtagaa ctcagagacc gaaaggcgag gattgaatat
     1201 gtaggccacc agcaccgtac ggtccagacg ctgcatcatc cagcgaagga tggtcgtctt
     1261 tccggtgccc acttcgccgg tcaccaccac caggcctttt ccactctcaa tcccatagtg
     1321 gaggtttgcc atcacctctg tatgtgacgg agtaaagtag atgaagcgcg gatctggcgt
     1381 cagcgcgaat ggcaactctt tcaggccgta gaattctgcg tacactcttg aacctccatc
     1441 gcgggaagtc gaaaacagct tcccgctttt ctgtatcagg cgcgtcccgt cgacataaac
     1501 atttcaaaca cgtgtgtcgc cctaagggcg agcgccagta aaggaatact ggcgatggtc
     1561 acagctatgc cggcagccag gagcaaccgg cggcggcgcg gaacagatcg cgcttcctgt
     1621 ggcgttagca actcgggcac cgaaaccaac accggaagat tggtgtagtg actggcatct
     1681 tcgctggttt ggatggtcag cagttttgga atctccatta ccccaaccag cagaaaccca
     1741 aacacaagtc ctattccgag tcccattaca gagagcatga agcgcttcgg agcaaccggt
     1801 ttggaaggca ggtttgcggc gtcaatcact tcgattgtct cgccctgctg ctgacttgca
     1861 gcgtctgccc cgaggctgat tctttgctgc tgtaccagga gggaatcgta ggctgccttc
     1921 ttcgtttgat actcgcgatc gagacggctc agctccactt ccgcaccggg aaccatgttg
     1981 atgcgctgca tgacgagtcc gatctgagtt tcgatctggc caatcaatcc ctgctgccgc
     2041 ttaatctcgc tatcaattag cttcagttcg ttttcgagag ccgagacctg caaatccgga
     2101 cggtctttca gtttttcctg cttctctcta atcttctgtt tccagtctgc gatcatgtcg
     2161 tccattgaat gctggactga atcaatctca gcctgttttg cgattacatc cggatgcttg
     2221 tcgcgcagct ctgtgcgcat gcgcgtcaat tgcgcctcga gatccgcctt acgagtggca
     2281 agctggcccc aggcaggcgt ggtcttggga tcggtagttg actctgcgat atcgtcttta
     2341 acctgctcga ccgacttctt aattgaaccc agttgcgagg ccattgctga ccgccggtct
     2401 tgtaatcgtc ccacttccgt gatgtaggcc ttctgctgat cccgcaagcc attaagttgg
     2461 ctcaacagtg actggccttc agccggaagg ttcccaaggt tccgagacat gaaatcgagt
     2521 cgttgtttat cgatcccatc caactcctcc ttggcttgac gcgcttgctg ctcgataaaa
     2581 ttcttggcgg aggtggtcga gttgatagtg tttttggttt gctcgtcgat gtatttactg
     2641 gcgagctctc tcgttaccat ctgagtcgtt tggggatcgc gatagcggta ggtgatgttg
     2701 aagccgttgg taatatcatt ccggctggta ttcacctcca ccttaatatc tcgccgcatt
     2761 gcgtcgatga tcgactccat cggctcgccg cgtaatcttt ccgtcttgta cagatcgtag
     2821 cggctgacta atggctccag tgagctgcgg ctggccacta cctgcgcgat gctcgtcaac
     2881 tgacgtgtca ggctatcttc ggtaattgac gggacgactg tgttggggag ggttgagggc
     2941 ttgaccacaa tcagggtagt tgactcgtac acgtcgggca ggcggtaaac cacccacgac
     3001 acggcagtcg taatcgcaat gatcggaagg atgatcaacc atttacgctt ccagattatt
     3061 ttggcgtatt cgcctggttt tcgctgccgg aattcaacgc tcatacaaca ctcctctttc
     3121 ttcttactcg cggaacttct gctctcaccc gacgatcctt acatggtcga gagagcctta
     3181 cgtgcttcat cagattcctt gaaaggtacc ttctctccca ggcgcaaggc cgtttccaac
     3241 tcgcgccgcg cggcatcctt atcgcctttg gctttcagcg ccatgccaag gtggaaacga
     3301 tagacggggc tagaaggtgc gtttgcattt tttgcgatcg actcatcgat gcttacggcc
     3361 ttctgcagtt ggtccactgc agcagaatga aggc
//
LOCUS       DASUEX010000275         2339 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1424085, whole genome shotgun sequence.
ACCESSION   DASUEX010000275 DASUEX010000000
VERSION     DASUEX010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1424085"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1518
                     /gene="kdpA"
                     /locus_tag="VFV61_04645"
     CDS             <1..1518
                     /gene="kdpA"
                     /locus_tag="VFV61_04645"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF015753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HEX4899797.1"
                     /translation="SVTFTGSAFNSAASFTTNTNWQSYVPEVTMSYLTQMAGLAYHNF
                     VSAAVGISLAVAFIRGIARREMETIGNFWVDITRSLLWVLLPFCIVGALVLVSQGTIQ
                     NLRPYDIAKILDQQTIEVDKKDAAGNVVINNDGTTAKETQVISDQVIAQGPLASQEFI
                     KQWGTNGGGFFNANSAHPLENPTPFTNLIALFSIFTISAGLTYTLGRMTGSQKHGWAV
                     WGAMAVLCLAGVTTAYWAEARGNPLLAGTDQAVSAKQSGGNMEGKEVRFGIASSALFA
                     TVTTAASCGAVNSMHDSFTPLGGMVPLANILLSEVIFGGVGAGMYGILIYIVLAVFIA
                     GLMVGRTPEYLGKKIESYDVKMAMLVVLVFPLMILIFAAISTVSSSFGTSQLNNAGPH
                     GLSEILYAFSSGTANNGSAFAGISANTLWYNTTIGIAVLVGRFFMIIPMLAIAGNLAR
                     KKTVPPSLGTFPVTTPLFAALLVSVIVIVGALTFFPALSLGPIVEHLLMNAGQTF"
     gene            1647..>2339
                     /locus_tag="VFV61_04650"
     CDS             1647..>2339
                     /locus_tag="VFV61_04650"
                     /inference="COORDINATES: protein motif:HMM:NF012350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit B"
                     /protein_id="HEX4899798.1"
                     /translation="MTRTPNKQAVWNAQIVKRAVADSFIKLNPRKMMANPVMFVVEVG
                     SLLTMLQLIRGIVAPIPGLTSTGFELQITLWLWFTVLFANFAEAMAEGRGKAQADNLR
                     KSKTETIAHRIVSGDLTEDIPAPQLRKDDLVIVSAGEFIPGDGEIVEGVASVDESAIT
                     GESAPVIREAGGDRSAVTGGTRVLSDQIKVRITSNPGETFIDRMIALVEGAERQKTPN
                     EIALNILLAGLTI"
BASE COUNT          517 a          642 c          648 g          532 t
ORIGIN      
        1 tcagtaacgt tcacgggctc cgctttcaac agcgccgcct ccttcacaac caacacgaac
       61 tggcagtctt atgtgcccga agtgacgatg agctacctga cgcagatggc aggtctggct
      121 tatcacaact tcgtctcagc agcagtaggc atttcgctcg ctgtcgcttt cattcgcgga
      181 attgcgcgcc gcgagatgga aaccattggc aacttctggg ttgacatcac ccggtcgctg
      241 ctctgggttt tactgccctt ctgcatagtc ggcgcgctgg tgctggtctc gcagggaacg
      301 atccagaacc tcaggcccta cgacatcgcg aagatcctcg accaacagac aatcgaggta
      361 gacaagaaag acgccgcagg caatgtcgta atcaacaatg acggcaccac cgcgaaagaa
      421 acccaagtga ttagcgatca ggttatcgcc cagggcccac ttgcatcgca ggagttcatc
      481 aaacagtggg gcacaaacgg cggtgggttc ttcaatgcca atagcgcgca tccactcgag
      541 aacccgacgc cttttacgaa cctgattgca ttgttctcta tcttcactat ctccgccgga
      601 ctcacttaca cccttggacg catgaccgga tcgcagaagc atggttgggc cgtctggggc
      661 gcaatggcgg tgctctgtct ggcgggcgtg actacggctt actgggcaga agcgcgtgga
      721 aacccgctgc ttgccggtac cgatcaagca gtcagcgcca agcagtcagg tggcaacatg
      781 gaaggcaaag aggtgcgctt cggaatcgca agtagtgccc tcttcgcgac ggtcacgact
      841 gcggcttcgt gtggcgcagt caactctatg cacgacagct ttacgccgct cggcggtatg
      901 gtaccgctcg ccaatatctt gctcagcgaa gtgatttttg gcggcgtcgg cgcgggcatg
      961 tacggcattt tgatttacat cgtgttggct gtcttcatcg ctgggctgat ggtcggacgg
     1021 acgcccgaat acctcggtaa gaagattgaa tcctacgacg tgaagatggc catgctggtg
     1081 gtgctcgttt ttcccctgat gattctgatc ttcgcagcga tctcgacagt gtcgtcgtcc
     1141 tttggcacgt cacaactgaa caacgccggg ccacacggac tgtccgaaat tctctacgct
     1201 ttcagttccg gcacggccaa caacggttcc gccttcgcgg gcatttcggc aaatacgctt
     1261 tggtacaaca caacgatcgg catcgcggtg ttggtcggtc gcttcttcat gatcatcccg
     1321 atgctcgcca tcgcgggaaa cctggcgcgt aagaagaccg tgccaccgtc actaggaaca
     1381 tttcccgtta caacaccgct gtttgcggca ttgctagtga gcgtcattgt gattgtgggc
     1441 gcgcttacat ttttcccggc tcttagtctg gggccgattg tcgagcacct gttaatgaat
     1501 gcggggcaga cgttttgagg aggaaggtct ttggtctttg gacttaggtc tttgtcttat
     1561 ttccgctgtg agtatgaagt cgcaactgta ttactaagtc aaagggccaa gaccaaaggc
     1621 ctaagaccta aaacggaatc aataccatga cgcgaacgcc gaataaacaa gcagtctgga
     1681 acgcacaaat cgtcaaacgg gcagtcgctg attctttcat caagctcaat ccgcgaaaga
     1741 tgatggccaa ccctgtcatg tttgtcgtcg aggttggcag cctcttaacg atgctgcaat
     1801 tgattcgcgg aatcgtcgcg ccgattcctg gtctgacgag tacggggttt gaattacaga
     1861 tcacactctg gctctggttc acggtgctgt ttgcgaactt cgcggaggct atggctgaag
     1921 ggcgtggcaa ggcccaggcg gacaacctgc gcaaatcgaa aaccgagaca attgcgcatc
     1981 gtatcgtcag cggcgacctg acggaagaca ttcctgctcc gcaactacgg aaagatgatc
     2041 tcgtgatcgt ctctgccggt gagtttattc ccggtgacgg cgagattgtt gaaggcgtgg
     2101 cgtcggttga tgagtcagca attaccggcg aaagcgctcc cgtgatccgc gaagcaggtg
     2161 gtgaccgttc tgccgtgacc ggcggcacgc gcgtcctttc agatcagatc aaggtgcgca
     2221 tcacctccaa tccaggcgag acctttattg accggatgat cgcactcgtc gaaggtgcag
     2281 agagacagaa gactccaaac gaaattgccc tgaacattct cctggcagga ctaacgatc
//
LOCUS       DASUEX010000276         3806 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_56568, whole genome shotgun sequence.
ACCESSION   DASUEX010000276 DASUEX010000000
VERSION     DASUEX010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3806
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56568"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="VFV61_04655"
     CDS             <1..339
                     /locus_tag="VFV61_04655"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF024897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEX4899799.1"
                     /translation="TSDTPFTNELRPGAVLRLPIAHADGNYICDDVTFAELKKNDRII
                     FNYCDKDGNITDASNPNGSLGNIAGICNAGRNVLGLMPHPERACEDLLGSSDGREVFS
                     SLAATLAAVA"
     gene            474..1190
                     /locus_tag="VFV61_04660"
     CDS             474..1190
                     /locus_tag="VFV61_04660"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX4899800.1"
                     /translation="MRKFLPLLIILAVLGIALGAGWYLTRPKASPPARPLSPQSSVNV
                     SATGSSPTPAALKVSQLGADPPHSIGPADAPVTLEEFGDFQCPPCGLLHPVLKDMEKE
                     FGSQLRVIFREFPLAQTHQHAINAARAAEAAGMQGKFWEMHDLIFTNQRTWKDAFDAR
                     PTFEEYAVQLGLDIAKFRRDVTSSAVDQRIALDGRRANAMGVNGTPTVFMNGREVPFE
                     SLPPERLRPLIQAELRAKGR"
     gene            1499..1948
                     /locus_tag="VFV61_04665"
     CDS             1499..1948
                     /locus_tag="VFV61_04665"
                     /inference="COORDINATES: protein motif:HMM:NF019499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HEX4899801.1"
                     /translation="MTGTITPTKTFTLKASKLYIAMAGLALIGLADAIYLTVQHVTGQ
                     SVRCTIVSGCSEVLSSSYASFNGVPLALIGAIAYFTVFSLATLAAFGYPLVGKLLAVV
                     TTLMFLTTLWLFYLQAFVIGHFCQFCLLSAAVTITLTVLVLIIARRR"
     gene            complement(2092..3702)
                     /locus_tag="VFV61_04670"
     CDS             complement(2092..3702)
                     /locus_tag="VFV61_04670"
                     /inference="COORDINATES: protein motif:HMM:NF015140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HEX4899802.1"
                     /translation="MKKHYARQFAIGVFAALLFMALAGASGIPVINSSSRAFAQGTPT
                     PSPTPDVDHCQINLNAASFSVKESEGQVMITVNRKCDDVRDSKVDFFTRNGSAAAGSD
                     FQFASGRVEFAPGETSKTIAINITNDGFSESTESFSLVLYDPGGSARLVNPSTAVVTI
                     LDDDEGGPTPTPTPSPSPTPSPSPTPDVEHCQISLSAPNFSVNEEAGSLAVTVNRACD
                     RERDSKVDFITRSGSASDRSDFTFAAGRLFFAPGEKAKTITILITNDALIEATETFEL
                     LLRDPGGSASLINPSGAVISIVDNDKSATAQNPVMQTDFFVQQHYADFLGRQADPAGF
                     NAWQEVINQCPAGNTDCDRVHVSSMFFRSPEFQERGYFVYRFYEVGFGRKPDFAEFMP
                     DIASVSGFFTDAEKEALKVAFIQALMARPEFANRYNALSDAAYVDELLRVSGLLSHWQ
                     RNDWVSDLQAGRKSRAQILREIAESPEVYQRFYNRAFVVMQYFGYLRRDPDSLYLDWV
                     RVLDDTGNPRTMIQGFVDSLEYKYRFGQ"
BASE COUNT          816 a         1066 c         1030 g          894 t
ORIGIN      
        1 acgagcgata cgccttttac caacgaattg cggccgggcg ccgtgctgcg ccttccgatc
       61 gcccatgccg atggcaacta catctgtgat gacgttactt ttgctgaact gaaaaagaac
      121 gatcgcataa tcttcaacta ctgcgacaag gacggcaaca ttaccgatgc gtcaaatccc
      181 aacggatcgc tcggcaacat cgccggaatc tgcaacgccg gacgcaatgt cctcggcttg
      241 atgccgcatc cggaacgcgc ctgcgaagac ttgcttggtt cgagcgacgg ccgtgaagtt
      301 tttagttcac tcgccgccac gctcgccgcc gttgcttgaa gacctcttct taatcaatgc
      361 tgtcccggaa aagtaaaatg gaaagtttac gttaacggca atctcgagga gaccgtgccc
      421 gacacggtcc agtgacttgc tcaaactagt tctagacatc cactgttcac attatgcgaa
      481 aatttctacc actgttaatc atcctggctg tgctaggtat cgctctgggg gctggttggt
      541 atctgacgcg tccgaaagcg agcccgccag cgcggccact ctctccccaa agtagtgtca
      601 acgtttccgc tacgggctcg tcgcccacgc ctgccgcgtt gaaagtgtcg caactcggcg
      661 cggatccgcc tcacagtatt ggcccggcag atgcgcctgt gacgctcgag gagtttggcg
      721 actttcagtg tccgccgtgt ggcttgctgc atccggtctt gaaggacatg gaaaaagagt
      781 ttggctcgca actgcgtgta atttttcgcg agtttcctct ggcgcagaca catcagcatg
      841 cgatcaacgc ggcccgcgca gctgaggctg cgggcatgca gggaaagttc tgggaaatgc
      901 acgacctgat ctttacgaat cagcggactt ggaaagacgc ctttgacgcg cgcccgacct
      961 ttgaagagta cgcggtgcag ctgggactgg acattgcaaa gtttcgacgc gatgtgacca
     1021 gcagcgctgt cgatcagcgc atcgcccttg atggcagacg cgcgaacgcg atgggcgtaa
     1081 atgggacgcc aacggtgttc atgaatggac gcgaggtacc gtttgaatct ctgccaccag
     1141 aaaggctacg gcctttgatt caagccgagc tcagagcaaa gggtcgctga tggcaaggtg
     1201 tacgctcgtc acttagaagt cagaacgcag aagtcaatgg ttagttgtta gtagttgata
     1261 gtcaatatgg ttttaaagtc ttgagctgga atcggctggg aagtctggag ccagagtctg
     1321 gaattcttga ggcagaatcc ggctggaatt cttacgccag aatcggctcg gctttgccgc
     1381 cggatgtgag gcgccggctc tgccgcgccg taagcgtctt gaaattctct ggggctgcgc
     1441 ccctagttcc cagtctaagc gcagagccga atatccaaca cctgacttct gagacagcgt
     1501 gactggcact attactccaa caaaaacctt cactctaaag gcgtcgaagc tttacatcgc
     1561 aatggccggc ctggccctca tcggattggc cgatgcgatc tacctcactg tccaacacgt
     1621 gaccggacaa agtgttcgct gcacgatcgt cagtggttgt tcggaagttc tgagcagttc
     1681 ctacgcttca ttcaacggag tccccctcgc cctcatcggc gccattgctt acttcactgt
     1741 ctttagcctg gcaacactgg ccgctttcgg atatccgctc gttggcaaac tgctggcagt
     1801 cgtcacaacc ctgatgtttc tcaccacgct ttggctgttc taccttcagg cttttgtgat
     1861 tggacacttc tgccagtttt gtttgctctc ggcggctgtg acgatcacgt tgacggtgtt
     1921 ggtgctgata atcgcccgcc gccgctgaag gttccctcgc agctggagcc ggcttgcctc
     1981 cgcatcacgg tcacctctta ctacaaaaac tggctcgcac gcgcatctcc cagcgcacgc
     2041 acgagccagc caaccgcgaa ccccgcttcg cggtaagttg aagtaccctc gctattgccc
     2101 gaagcgatac ttgtactcga gcgaatccac gaacccttga atcattgttc gcgggttgcc
     2161 cgtgtcatcg agcacgcgca cccagtccag atatagggaa tctgggtctc ggcgcagata
     2221 cccgaagtac tgcatcacga cgaaggctct gttgtagaag cgctgataga cttccgggct
     2281 ttcagcgatc tctctgagaa tctgcgccct gctctttctt cccgcctgga gatcactaac
     2341 ccagtcattg cgctgccaat gacttagcag acctgagact cgtagcaatt catccacgta
     2401 ggcagcgtct gacagcgcat tgtatctgtt ggcaaactca ggccgggcca tcagcgcctg
     2461 aatgaatgcc accttcagcg cctccttctc tgcatctgtg aaaaaaccgc tgacactggc
     2521 gatgtcgggc atgaattccg caaagtccgg tttacgaccg aagcccacct cgtaaaagcg
     2581 gtagacaaaa taaccgcgct cctgaaactc cggcgagcgg aagaacattg acgagacatg
     2641 aactcgatcg cagtcagtat ttccggctgg gcattgatta attacttcct gccaggcgtt
     2701 aaagcccgcc gggtcagcct gccgacctag aaaatccgcg tagtgctgtt ggacgaagaa
     2761 atcagtctgc attacagggt tctgggccgt ggcgcttttg tcattatcga caatgctgat
     2821 cactgctcca ctgggattga tcagggacgc gctgccgcct ggatctctca acagcaattc
     2881 gaaagtctcg gttgcttcga tcaatgcgtc attcgtgatt aagattgtga tggtcttggc
     2941 cttctcgccc ggggcaaaga aaagcctgcc cgccgcaaac gtgaagtcag atcgatccga
     3001 ggctgagccg ctgcgcgtta tgaaatcgac tttggaatca cgctcgcgat cacaggcgcg
     3061 atttacagta actgcgaggc tcccagcctc ctcattcacg ctgaagttcg gcgcactgag
     3121 actgatctgg cagtgctcaa cgtcgggtgt tggactcggc gacggcgtgg gactgggcga
     3181 cggagttggc gttggcgttg gccctccctc gtcgtcatcc agaatcgtta caactgcggt
     3241 actcgggttg acgagtcggg cgctgccacc tggatcgtag agtacgaggc taaaactctc
     3301 ggtactctcg ctgaagccat cattagtgat gttaatcgcg atggttttgc tggtttctcc
     3361 cggtgcgaat tcaacccgac cggaggcaaa ctgaaagtcg gagcctgctg ccgccgaacc
     3421 gttgcgggta aagaaatcca cctttgaatc acgcacatcg tcacactttc gatttacagt
     3481 gatcatgact tgcccttcac tctctttaac cgagaatgac gcagcgttta ggttgatctg
     3541 gcagtgatct acgtctggcg tcggggacgg tgttggggtt ccctgcgcga atgcccgtga
     3601 cgaactgttg attaccggaa tgcccgaggc gccagccagg gccataaaga gtagtgcggc
     3661 gaaaacgccg attgcgaatt gtctagcgta atgtttcttc attttgattc tccctggtat
     3721 cgcccgagcg gtggatccgt cacggcttta attcttcggt cagacagtga ggggcgtgag
     3781 caaataaata catgtgagtt tttttg
//
LOCUS       DASUEX010000277         2890 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1179261, whole genome shotgun sequence.
ACCESSION   DASUEX010000277 DASUEX010000000
VERSION     DASUEX010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2890
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1179261"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..359
                     /locus_tag="VFV61_04675"
     CDS             <1..359
                     /locus_tag="VFV61_04675"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: protein motif:HMM:NF013461.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HEX4899803.1"
                     /translation="KKMEQHNAACWLVNTGWSGGPYGVGHRMKISHTRAMIRAILSGS
                     LANVETRPDPIFGVNVPVSCPEVPAEVLQPRNTWTDKDAYDQKARELARRFNENFKKY
                     ESGVSEAVRAVAPKAD"
     gene            complement(381..731)
                     /locus_tag="VFV61_04680"
     CDS             complement(381..731)
                     /locus_tag="VFV61_04680"
                     /inference="COORDINATES: protein motif:HMM:NF023395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HEX4899804.1"
                     /translation="MSESSCLFCRIIAGEIPAELVHQDKQCVVIRDVNPQAPTHLLVI
                     PREHLESLNDAGQPDEGLVGHLLRMAARMANEQGLSESGYRTVINTGAGAGQSVFHLH
                     VHVLGGRPMSWPPG"
     gene            complement(767..2092)
                     /gene="murA"
                     /locus_tag="VFV61_04685"
     CDS             complement(767..2092)
                     /gene="murA"
                     /locus_tag="VFV61_04685"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF006873.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEX4899805.1"
                     /translation="MDKFLISGGCALRGSVAISGAKNSALPCMAAALLTAETVTLHNL
                     PYARDIITQRRLLEDLGATVLTPELRTHKISAAHIEVFEAPYELVKTMRASVLALGPL
                     LARFGKAKVSLPGGCAIGTRPIDLHLKGFELLGAEVRLEGGNVVARAPQDGRLIGATV
                     NFDKVTVTGTENLLMAATLARGKTVIHNAACEPEISDLCELLNRMGARVRGGGTPTIE
                     IEGVEALGGAQHTIIPDRIETGTFIVAAAITDGELIIKDCQPEHLTSVIDQLHEVGVE
                     IEELNQSTLRVKRGTTLRAKNVTTEPHPKFPTDMQAQYMALMTQAEGESIITETVFEN
                     RFMHASELQRMGAHIHLNGRTATVTGPTKLTGAPVLASDLRASASLVLAGLVAQGETV
                     IERVYHIDRGYERIETKLRAVGANIQRVRESVTAPLSEAAAPDAAALAS"
     gene            complement(2217..>2890)
                     /locus_tag="VFV61_04690"
     CDS             complement(2217..>2890)
                     /locus_tag="VFV61_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899806.1"
                     /translation="KMPAGGLLTVDASRNGGVAIKGWDQNEILVRARVQAAAPSQGEA
                     DQLVKQIRIETSGGKVHAVGPESQKNYQWNVSYEIFVPRRSDLSLEAYNGGISITDVT
                     GRIEFTGHNGGVVLRRVGGTVRGGTMNGGLVVELDGTSWSGEELDVKTTNGGIVMAIP
                     ENYSAHLETGTVNGHLNIDFPVTVQGRIGRELAVNLGNGGPTVRAMTTNGGIKIKRTG
                     TDSSN"
BASE COUNT          594 a          839 c          777 g          680 t
ORIGIN      
        1 gtaagaaaat ggaacagcac aacgctgcct gctggttggt caacacgggt tggagtggtg
       61 ggccttatgg cgttgggcat cgcatgaaga tctctcatac gcgcgcaatg atccgggcaa
      121 tactgagcgg atcgctcgcc aatgtggaaa ccagacctga tcccattttt ggtgtgaacg
      181 tccccgtttc ctgtccggaa gttccggcgg aagtgcttca gccgcgcaat acctggaccg
      241 ataaagacgc atacgatcag aaggcgcgcg aacttgcgcg acgtttcaac gagaacttca
      301 agaagtatga aagtggggtt tcggaagcgg tgcgcgctgt tgcacccaaa gccgattagc
      361 cttacgccgc ggagtcgcgc ttagcctgga ggccagctca ttggccgccc acccaggaca
      421 tgcacgtgta gatggaagac tgattgaccc gcccccgcgc cggtattgat gacggtgcgg
      481 tagccactct cgcttaatcc ctgctcattc gccattcgcg cggccatgcg aagcaaatgt
      541 cccaccagac cttcgtcagg ttgccccgcg tcatttagcg actccagatg ctcgcgcgga
      601 atcaccagca agtgagtagg tgcttgcggg ttaacgtcgc gaataacgac gcattgcttg
      661 tcctgatgga ccaattcggc cggtatctct ccagcaataa ttcgacaaaa caaacaactt
      721 gattctgaca ttctttcgtc ccctgggtcc gatgttagcc ttagtcttac gacgccagcg
      781 ctgcggcatc tggtgcggca gcttccgaca gcggcgccgt aactgactct ctgacgcgct
      841 gtatgtttgc tccaactgcg cgcagcttcg tttcaattcg ttcgtagccg cggtcgatgt
      901 gataaacgcg ctcaatcacg gtttctccct gtgctaccaa ccctgccagc acgagtgatg
      961 cggacgcgcg tagatctgag gcgagcacag gtgctccggt caatttcgtc ggtccggtta
     1021 ccgttgcagt tcggccgttc aagtgaatgt gcgcgcccat acgctgcaac tctgaagcat
     1081 gcatgaaacg gttttcaaaa actgtttccg tgattattga ttcgccctca gcctgagtca
     1141 tcagcgccat gtattgggcc tgcatgtcag tgggaaattt aggatgcggc tcagtggtta
     1201 cgttctttgc tcttagcgtt gtcccgcgtt tcacacgcaa ggtgctttga tttaattcct
     1261 caatctcaac cccaacctca tgcaattgat cgataacact cgtcagatgt tcgggctgac
     1321 agtccttgat tattaactcg ccgtccgtga tcgcagctgc gactatgaaa gttccagtct
     1381 cgatgcggtc aggaataatc gtgtgctgcg caccaccaag tgcttcgacg ccctcgatct
     1441 cgatcgtcgg cgtaccgccg ccacgcacgc gcgctcccat ccggttcagc aactcgcaga
     1501 gatcgctgat ctccggttcg caggcggcgt tatggatcac ggtcttgcct cgtgcgagag
     1561 tcgctgccat caacagattc tcagtaccag tcaccgtgac cttatcgaaa tttacggtgg
     1621 cgccgattag ccgtccgtcc tggggcgcgc gcgccaccac gtttccacct tcaagtcgca
     1681 cctcggcacc caatagttcg aaacccttca gatgaagatc aatgggtcga gtgccgatgg
     1741 cacagcctcc tggaagcgag accttggctt ttccaaagcg cgccagtaat ggtcccaggg
     1801 ccagcaccga ggcgcgcatg gtttttacca gttcatatgg cgcttcaaac acctcgatat
     1861 gagcggcgct gatcttgtgc gttcgaagtt ctggagtgag aacggtagcg ccaagatctt
     1921 ccagcaggcg gcgctgcgtg atgatgtcgc gcgcataggg aagattatgg agggttactg
     1981 tttcggccgt cagcagggca gcggccatgc agggcagggc cgagttcttc gcgccgctga
     2041 ttgctacgga gcctcgcaat gcgcagccac cggaaatgag aaatttgtcc acggctccaa
     2101 cagattaaat taaaaacctt aaaggtaaaa gggatggagt tgcggcagga ccaaatgaca
     2161 aggcgcggca agtttcttaa ctcgccgcgc cctacggtgt tgcttccctt tgggttttag
     2221 ttgctcgaat cggtacctgt acgtttgatc ttgatgccgc catttgtggt catcgcccgc
     2281 accgtgggcc cgccgttgcc gaggttaact gccaactcac ggccgatccg accctgcacg
     2341 gtcacaggaa aatcgatgtt gagatgaccg ttaaccgtcc cagtctcgag atgggcggaa
     2401 tagttttccg gtattgccat cacaatgccg ccgttggtcg ttttcacgtc aagttcttcg
     2461 ccgctccaac tggtgccatc tagttcgacg actaatcctc cgttcatcgt gccaccacgc
     2521 accgtaccac cgacgcgtcg cagcacgacg ccgccgttat gcccggtgaa ttcgatgcgg
     2581 cccgtgacgt cagttatgga aatgcctcca ttgtaagctt ccagcgaaag gtcggaacgt
     2641 cgcggaacaa agatttcgta actgacattc cactgatagt tcttctgact ttccggaccg
     2701 acagcatgaa cttttccgcc gctggtttcg attctgatct gcttcacgag ttgatcagct
     2761 tccccttgcg aaggtgcagc ggcttgcacc cgcgccctta caaggatctc gttctggtcc
     2821 caccctttga tggcgactcc accgtttcgg ctagcgtcaa ccgtgagcag accgccggcg
     2881 ggcattttct
//
LOCUS       DASUEX010000278         5509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1546147, whole genome shotgun sequence.
ACCESSION   DASUEX010000278 DASUEX010000000
VERSION     DASUEX010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5509
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1546147"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..916)
                     /locus_tag="VFV61_04695"
     CDS             complement(<1..916)
                     /locus_tag="VFV61_04695"
                     /inference="COORDINATES: protein motif:HMM:NF026220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HEX4899807.1"
                     /translation="MGRQAKQQIGNRQSAIGNSRCCRLPSASCRLFFGACLLLLAIST
                     SALAQSGAWVRQTSGSLSWLHGVFFLDQNSGWAVGSKGALLTTEDGGKSWKARPKPSD
                     DVLRDIYFADPLNGWIVCERNIYQLKSNTDPRTYLMNTTDGGANWQRVVLPGADVNAR
                     LVRAVFSPGGSGWAFGEFGAVYKSSAGDANWVRLQSPTRRLLLGGTFIDDNRGWLVGA
                     GATILQTSDGGETWHVSQLADATGIRFNATSFVGNRLGWAVGSGGSIYRTVNGGRTWQ
                     PQRSGVSVDLYDVKFLDALEGWAVGAEGT"
     gene            complement(1035..1934)
                     /locus_tag="VFV61_04700"
     CDS             complement(1035..1934)
                     /locus_tag="VFV61_04700"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HEX4899808.1"
                     /translation="MKAVNRFSGFALTAGLLLSLSVAVPAQQPEPAENQTRPRVSSTA
                     SPTPPAVPDKSKESKPATLPTGERLEPDDVKHSPSDVPPEMQANRQEQLSEDNDVVPY
                     YNNFFNTYRLGPEDVISVNVFGQERYSRAGIVVPPSGRISLALIPGGVFVNGKTVDEV
                     AEIIKKQYDEYIIDPQVTVSLEKGFSYRYAVIGDVAQPGIRVMNRRLTVTEAITEAGG
                     VLPTGNRSKVVVLRRQPTGLLSPIAVNVSAIYKGKVPDNTYLVPGDQIIVPGNKLKTL
                     QKIMSLSPIMTFARIFTGGGLPF"
     gene            complement(1960..3348)
                     /locus_tag="VFV61_04705"
     CDS             complement(1960..3348)
                     /locus_tag="VFV61_04705"
                     /inference="COORDINATES: protein motif:HMM:TIGR03013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03013 family XrtA/PEP-CTERM system
                     glycosyltransferase"
                     /protein_id="HEX4899809.1"
                     /translation="MRASRLNARTVLLLLAEATVIFGAIIGAVDLRFGLDGARYELIE
                     RHGLLKAALATGFCLTTFYLFDLYDFIVMHDRRELVLRLVQALGLAWIALALMFYVFP
                     YLMIGRGVALIALPLALALMVSWRLSIHWFLGHPDFGERILIVGSGSLAVEVAREVLD
                     RPDAGYRIVGFVGTDSELLGKSLINPRVIGITSELAEVVKRENIDRIVVAMGERRGLL
                     PTEKLLQLSLAGTVSIEEGATFYERITSRVSLNMIRPSWLIFSGRGRQGRLAGPVRSF
                     VHRFVALLGAILSLPLVLLTAILIKLESRGPIFYRQERVGTNGKPFVLLKFRSMRVDA
                     EQDGPVWASKNDDRTTRVGRIIRKIRVDEIPQFWNILRGEMSFVGPRPERPHFVAQLA
                     QEIPYYEQRHLIPPGLTGWAQIKYPYGASIEDARQKLQYDLFYVKNQSLVLDAIVLFE
                     TIKIILFGRGGQ"
     gene            complement(3345..4439)
                     /locus_tag="VFV61_04710"
     CDS             complement(3345..4439)
                     /locus_tag="VFV61_04710"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HEX4899810.1"
                     /translation="MGRVFDAIRRSSTTENSGVQNPRPSQSSQSSEQRNGGDNQIPTA
                     RQIEEELFRGSSIMSQNNALHSSASTAHTADAPGGSALPGGMTSRAVGATLGAAGATR
                     AAGFVSYDISAARVEPHLVAVSQPRSGYCEQFRSLRTRLLQAGERRQMRTFVITSVGI
                     GEGKTLTALNLAWLLAQTDGVRALVIDSDLRQPCATDYLGIDAPIGLSEVLGGELGLE
                     EAIVRLDPAGLFLLPGGRARDDVAELLSGPTFARLIADCRRMFDYIIIDAPPMGIFSD
                     ASVLISRADAALLVVRAGKTRYAQLDKLIEQLPKERLLGVVLNRADEKTDSSAYYYQQ
                     RYSHRDRALPAEASLTLPNETSNEEVGIVN"
     gene            complement(4512..5468)
                     /locus_tag="VFV61_04715"
     CDS             complement(4512..5468)
                     /locus_tag="VFV61_04715"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX4899811.1"
                     /translation="MYAEFYGLKELPFALTPDPRFIYFTPSHIEVMANLHYGIESGKG
                     LVVVTGEVGTGKTTILRWMMQRLDRTVLVAYIFNPRLSVPEFYQHVAKLLGVQNWETK
                     SDLLLELGRALETRHSRGLRTVLIVDEAQGLSSHVLEEIRLLSNFESNTAKQLQIVLT
                     GQPELRDVLNNPDLRQLKQRIALRCVIKPLPTIEETERYIISRLLVAGAERTHIFSPA
                     AVDFIYRCSDGIPRNVNNLCDNSLLAGFAAGKETISRDLVEEVAETFDMLPSSDRGMQ
                     PGEQKEAPAHIFSPASRAELWQAGTAAPESEPELDLPMPVEH"
BASE COUNT         1261 a         1561 c         1496 g         1191 t
ORIGIN      
        1 ctgttccctc agcgcccact gcccagcctt caagcgcgtc gagaaatttg acgtcataga
       61 gatccacaga aacaccggag cgctgcggtt gccaggttct gccgccattg acggtgcgat
      121 agatactacc gccgctgcca acggcccaac ccaggcgatt ccctacgaac gaggtggcat
      181 tgaagcgaat gccggtcgcg tccgcgagtt gtgagacgtg ccaggtttca ccaccatcgg
      241 aagtttgcag aatcgtggcc ccagctccga ccaaccagcc gcggttgtcg tcgatgaacg
      301 taccgccgag taaaagacgg cgcgttggtg attgcagccg gacccaattg gcgtcacccg
      361 ccgacgactt ataaaccgcg ccgaattctc caaaggccca gccgctgccg ccggggctga
      421 agaccgcgcg cacgagccgg gcattaacgt cagcgccggg gagcaccacg cgctgccaat
      481 ttgcgccacc gtcggtggtg ttcattagat atgtgcgcgg gtccgtgttg gacttcaact
      541 ggtagatgtt gcgctcgcaa acgatccaac cattgagcgg gtctgcaaaa tagatatcgc
      601 gtaagacgtc gtcgctcggt ttaggacgag ccttccacga tttgccacca tcctcggtgg
      661 taagcaacgc acccttactg ccaactgccc aaccgctatt ttgatcgaga aagaaaactc
      721 cgtgcagcca tgacagcgag ccggaggtct ggcgcaccca ggcgccgctt tgcgctaacg
      781 ctgaggtgga gatagcaaga agcaggagac aggcgccgaa gaacagacgg caggaggcgg
      841 atggcagacg gcagcatctc gaattgccga ttgctgattg ccgattaccg atttgttgct
      901 tcgcctgtcg acccattacg tgatcgccaa ttggagaggg gattgccgac gtcgcctggc
      961 atcagctcgc gggttccaaa ggcttagatc cctcgaatgc taatcggcaa tcggtaatcg
     1021 ccactcggca gtttctagaa cggtagtccg ccgcccgtga agattctggc aaaagtcatt
     1081 attggactga ggctcataat cttctggagg gtcttcaact tgtttccggg gacaataatt
     1141 tgatcgccgg gcacgagata cgtgttatca ggaaccttgc ctttgtaaat cgccgagaca
     1201 tttactgcaa tcggggacaa caagccggtg ggttgccggc gcaaaactac taccttgctg
     1261 cgattgccgg tcggcaagac accgccagct tcggttattg cttccgtaac cgttagtcgc
     1321 cgattcataa cgcgaatgcc gggttgcgcc acatcgccaa tcaccgcgta gcgatatgag
     1381 aatcccttct ccagcgaaac cgtcacctgt ggatcgatga tgtattcgtc atactgtttc
     1441 ttgattattt cggccacttc atccacggtc ttgccgttga cgaaaactcc tccgggtatc
     1501 aacgcgaggg aaatcctccc gcttggcgga actactatgc cagcacgcga ataccgctct
     1561 tgaccaaaga catttacgga aatcacatct tccggtccga gtcggtaagt gttgaaaaaa
     1621 ttgttgtagt aagggacgac gtcgttatct tccgacaact gctcctgacg gttcgcctgc
     1681 atttccggag gaacatcgga aggtgagtgt ttcacgtcgt ctggttcaag ccgttctccg
     1741 gtgggcagtg ttgccggctt gctctccttc gacttgtccg gcactgcggg cggagtagga
     1801 cttgccgtac tcgacacgcg tggtcgcgtt tgattttctg cgggctccgg ctgctgagcc
     1861 ggaaccgcaa cgctgaggga gagtaagaga cccgccgtta aggcgaaacc cgaaaacctg
     1921 ttcactgctt tcatcactgc ctccaaaaaa cgcggcgctt tattgtcccc cgcgtccgaa
     1981 aagaatgatc ttaatcgtct caaacaatac gatggcatcg agtaccagac tttgattctt
     2041 tacgtagaaa agatcgtatt gcagcttctg ccgcgcgtct tcgattgatg caccataggg
     2101 atacttgatc tgggcccatc cggttagacc cgggggaatt agatgccgtt gctcgtagta
     2161 gggaatttcc tgggcaagct gggcgacaaa gtgagggcgt tccggacgcg gacccacaaa
     2221 gctcatctca ccacggagga tgttccaaaa ctggggaatc tcatcaactc gaatcttgcg
     2281 aataatccga ccgacgcgag tagtgcggtc atcgtttttg ctggcccaga cgggcccgtc
     2341 ctgttcggcg tcgactcgca tcgaacggaa cttaagcagc acaaagggct tgccattggt
     2401 gcctactcgt tcctgccggt agaagatcgg cccgcgtgat tcaagcttaa ttagaatcgc
     2461 cgtgagcaag acgagcggta acgagagaat tgcacctaag agggccacaa agcggtgtac
     2521 gaaactgcgc accggcccag ccagtcgtcc ttgccggcct cgtccggaga agatcagcca
     2581 cgaaggtcga atcatgttca gcgatacacg actcgtgatg cgctcgtaga aagtagctcc
     2641 ttcttcgatc gagacggtgc cggccaagct tagttgtaga agtttttccg tcggcagcaa
     2701 gccacgccgc tcgcccatcg ccaccacgat gcgatcgatg ttttcacgtt tcacaacttc
     2761 tgccagctcc gaagttattc caatcacgcg cggattgatt aggctcttac ccagcaactc
     2821 cgaatcagtg ccgacgaagc cgacgatgcg atatccggcg tcgggtcgat ccagaacttc
     2881 acgcgcaact tcgaccgcga gacttccgga gccgactatc aaaatccgtt cgccgaaatc
     2941 gggatgaccc aggaaccagt gaatgctcaa gcgccagctc accatcagcg ccaatgccag
     3001 aggcagcgcg atcaaggcca caccgcgtcc gatcatcaag taggggaaga cgtagaacat
     3061 aagggcgagc gcgatccacg ccagccctaa agcctgcacc agccgcagta ccaactcgcg
     3121 acgatcgtgc atgacaatga agtcgtaaag atcgaacagg taaaaagtgg tcagacaaaa
     3181 tcccgtggcc agggcagctt ttagcaagcc gtgacgttcg atgagttcgt aacgagcgcc
     3241 atccaggcca aaccgcaagt ccacagcacc gattatcgcg ccgaagataa cggtggcttc
     3301 ggcgagtagt aaaagtacgg tgcgggcatt gaggcgtgag gctctcaatt gacgattcct
     3361 acctcctcat tcgacgtttc attggggagc gtcagcgaag cttcggccgg caacgcgcga
     3421 tcgcggtggc tgtagcgttg ctggtagtag taagcacttg aatcggtctt ttcgtcggcg
     3481 cggtttagaa ctacgccaag cagcctttcc ttaggcaatt gttctattag tttatccaac
     3541 tgggcatacc gagtcttacc cgcgcgcacc accagcaagg cagcatcggc gcgactgatc
     3601 agcacactgg cgtcagaaaa gattcccatc ggtggggcat cgatgatgat gtagtcgaac
     3661 atgcgccggc aatcggcaat caaacgcgcg aacgttggac ccgaaagtaa ttcagccacg
     3721 tcatcgcgtg cgcgacctcc tggcaagaga aacagccccg cgggatcaag gcgcacgatg
     3781 gcctcttcta atccaagctc acccccgaga acttctgaga gtcctatggg cgcgtcaatg
     3841 ccaagataat cagtggcgca cggctgccgc aggtcgctgt caatcaccag cgctcgcacg
     3901 ccatcggttt gggccagcaa ccaagcgagg ttaagggcgg taagagtctt tccttcaccg
     3961 ataccgacgc tggtaatgac gaaagtgcgc atttgccgac gttcgccggc ttgcaatagt
     4021 ctcgtacgaa gcgagcgaaa ctgctcgcag taaccggaac gaggttgact caccgcaacc
     4081 aggtgaggct caacgcgcgc cgcggaaatg tcataagaga caaatcccgc ggcacgcgtc
     4141 gccccggcag cgcccaaagt tgccccaaca gcacgcgaag tcattccgcc ggggagtgcc
     4201 gacccgccgg gtgcatccgc agtgtgcgca gtggacgctg aactgtgcag cgcattgttt
     4261 tgcgacatta ttgaagagcc tctaaataat tcctcttcaa tctgacgcgc ggttgggatt
     4321 tgattgtccc caccgttacg ctgctcagaa ctttgtgaac tctgtgatgg tcgtgggttc
     4381 tgaacaccac tattttccgt ggtgctcgaa cgcctgattg catcgaaaac tcttcccatc
     4441 agttctcctt cttacttctg gcgacctgtt accggtcgct ggcattgttt cctgttcctg
     4501 ctcaatcaaa ctcaatgttc gacgggcatc ggtaaatcca attccggttc gctctccggc
     4561 gcagcggtac cggcttgcca taattcggcg cgactcgctg gcgaaaaaat atgtgctggt
     4621 gcttcttttt gttccccagg ttgcatgccg cgatcagaag acggcagcat gtcgaatgtc
     4681 tccgccactt cttcaaccag gtcccttgag attgtctcct tgcctgcggc aaagccggcc
     4741 agcaaagagt tgtcacaaag attgtttaca ttgcgcggaa tgccatctga gcagcgatag
     4801 ataaagtcga ccgccgcggg cgaaaagatg tgggttcgct cggcgccggc tacgagtaag
     4861 cgtgaaatga tgtaacgctc agtctcttct atcgtcggca acggcttaat cacgcaacgc
     4921 aaagcgatgc gttgtttcaa ttgtcgcaag tcgggattgt taagcacatc tcgcagttcc
     4981 ggctggccag tcaaaacgat ttgcagttgc ttggcagtat tagattcgaa gtttgaaagc
     5041 agtcgaatct cctcgagcac gtgcgatgag agtccctgag cttcgtcaac gatgagcaca
     5101 gtgcgcagcc cgcgcgagtg acgtgtttcc agagcgcgac ctaattccag cagcaagtcg
     5161 gacttggttt cccagttttg cacacccaaa agcttcgcca catgctgata aaactccggt
     5221 acggagaggc gcggattaaa gatgtaagca acgagtacgg tgcggtcgag tcgctgcatc
     5281 atccagcgca agattgttgt cttgccggtt ccgacttctc ccgtgactac cacgagaccc
     5341 ttgccgctct cgattccgta gtgaaggtta gccatcactt cgatgtgcga cggggtgaag
     5401 tagataaaac gcggatccgg tgtcagcgcg aatggcaact ctttcaatcc gtagaattct
     5461 gcgtacactt tctaaacctc catgaacaga ctctcaggct tggacctga
//
LOCUS       DASUEX010000279         3227 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1294497, whole genome shotgun sequence.
ACCESSION   DASUEX010000279 DASUEX010000000
VERSION     DASUEX010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3227
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1294497"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2012
                     /gene="uvrA"
                     /locus_tag="VFV61_04720"
     CDS             <1..2012
                     /gene="uvrA"
                     /locus_tag="VFV61_04720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEX4899812.1"
                     /translation="TVLEVDPAKLIPDANVPANKLDFMGPADRQGSAYLKSALTAVIN
                     YFKADSRLPFNELLKEVREGFFEGINGQLNFRQGAYSYQSRWKGALHWLRDRLNEAPS
                     EKVRTTLEEMVAPRTCPECKGKRLRADSLAVRFGERGISEYTTMPIEDASAAFAGIKL
                     NAREELIAGPILREIVNRLSFLNTVGLGYLTLDRPSSTLSGGEGQRIRLATQIGSQLR
                     GVLYVLDEPSIGLHPRDNQKLLETLCALRDLGNTVLVVEHDEETIRRADYVVDLGPGA
                     GAHGGEVVASGTAENVADNPNSITGLYLRGKNKIEVPAERRVPNGKHLIIHGARANNL
                     KEIDVTFPLGLLTVITGVSGSGKSTLVGDILYPALARHLYGSLAEPGTYETIEGLDNV
                     DKVIEIDQSPIGRTPRSNPATYTGLFTPLRELYAMLPESRERGYKPGRFSFNVKGGRC
                     EACEGDGMKRIEMNFLPDVYVLCDVCRGARYNRETLAVKFKAHSIAELLDTTVEEALP
                     LLENIPQIKQKLQTLLDVGLGYIKLGQSATTLSGGEAQRIKLAKELSKRATGRTVYIL
                     DEPTTGLHFADVHKLLDVLQRLVALGNSVLVIEHNLDVIKSADYIIDLGPEGGEHGGR
                     IVASGTPEEVARVRRSHTGQVLKPLLNGRLTRNGNGVRGEDKKIA"
     gene            2064..3029
                     /locus_tag="VFV61_04725"
     CDS             2064..3029
                     /locus_tag="VFV61_04725"
                     /inference="COORDINATES: protein motif:HMM:NF044507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899813.1"
                     /translation="MSIYDDLGPLSLENVSTYALAGRPGKVSLKDFAHPIEQGSSLSD
                     FLHGLPNILAVQNLRELGVQIRRARDLGKPIIWGIGGHVIKTGLAPLLIQLMQQGFVT
                     AIAANGSVIVHDSEIALTGSTSEDVDATLGEGVFGGAEETGKLLNRAAGEGARDTIGL
                     GEAVGRALVGLNPQHQNVSLLCAAYNARLPVTIHLTIGGDIVHFHPQANGAELGATSH
                     TDFRLLAELVRRMNGGGVFLNIGSAVVLPEVFLKCITLVRNLGHELSDITTANFDFIQ
                     SYRPLTNVVRRPTAAGAGRGYSIIGHHELTLPLLSAELICSNGSS"
BASE COUNT          740 a          876 c          873 g          738 t
ORIGIN      
        1 gaacggtgct cgaagttgat cccgcgaagc tgattcctga tgcgaatgtc ccggcaaaca
       61 aacttgactt catgggtcca gcggatcggc agggttctgc ttacctcaag agcgcgctga
      121 ctgcagtaat caattatttt aaagccgact cgcggttgcc cttcaacgaa ttgctcaaag
      181 aagttcgcga aggattcttt gaagggatta acgggcagtt gaatttccgt cagggtgctt
      241 attcatatca gagtcgctgg aagggggcgc tgcactggct gcgtgatcga ctcaacgaag
      301 caccatcgga gaaggtgcgg acgaccttgg aggaaatggt cgcacctcgc acctgtcccg
      361 agtgcaaagg aaaacggtta cgcgcggatt cgctcgcggt tcggtttggc gaacgcggga
      421 tttctgagta cacgacgatg ccaattgaag atgcatcagc agcgttcgcg ggcatcaagc
      481 tcaatgcccg cgaagagctg atcgccggcc cgatcctgcg cgaaatcgtc aatcggttga
      541 gtttcttaaa cacggtcggt ctgggttatc tcacactgga ccgaccttca tccacacttt
      601 cgggtggcga agggcaacgc attcgtctgg ctacgcagat tggttctcag cttcgaggcg
      661 ttctttatgt tctcgatgaa ccgtcgatcg gattgcatcc ccgcgacaac cagaaactgc
      721 tggaaacgct gtgcgccttg cgcgacctcg ggaacacggt tttggttgta gaacacgatg
      781 aggaaacgat tagacgtgcg gactatgttg ttgaccttgg tcccggtgcg ggcgcacacg
      841 gcggcgaagt tgtggcgagc ggaaccgccg aaaatgtcgc tgacaacccc aattcgatca
      901 ctggtctcta tctgcgcgga aaaaacaaga ttgaagtccc ggcggagcga cgcgtcccta
      961 acggaaagca cctcatcatt cacggggccc gcgccaacaa cctcaaagag atcgacgtca
     1021 cttttccact aggtctcttg actgtgatta ccggtgtctc cggatcagga aagtcgactc
     1081 ttgtgggcga catactatat cccgcgctgg cgcgtcactt gtacggttca ttggcagagc
     1141 ccggtaccta cgaaactatc gagggtctcg acaatgtcga taaagtgatc gaaatagatc
     1201 aatcgcccat tggccggacg ccgcgttcaa atcctgcaac ttacacggga ctctttactc
     1261 cgctacgcga gctctacgca atgctgccgg aatcgcgcga gcgaggctac aaacctggcc
     1321 gtttctcttt caatgtcaaa ggtggccgct gcgaagcttg cgaaggcgac ggcatgaagc
     1381 ggattgagat gaattttctg cccgatgttt atgtgctctg cgatgtctgc cgcggtgcca
     1441 ggtataaccg tgagactttg gcggtcaagt ttaaagccca ttcgatcgcc gagttactgg
     1501 atacaacagt ggaagaagcc ttgccgctgc tggaaaacat tcctcagatc aagcagaagc
     1561 tacaaactct tcttgatgta ggactcggat acattaagct gggtcaatca gcgaccacac
     1621 tgtccggtgg cgaagcgcaa cgaatcaagc tcgctaagga gttatctaag cgtgccacgg
     1681 gaaggactgt ctacattctg gatgaaccaa ccaccggact tcatttcgct gacgttcata
     1741 aactactcga cgtgttgcag cgcctggtgg ccctgggcaa ttccgtttta gtgattgaac
     1801 acaatctgga tgttattaag agcgcagatt acattatcga cctcggcccc gaaggcggag
     1861 agcacggagg cagaatcgtc gcttccggaa ccccagaaga agtggcacgc gttcgccgct
     1921 cacataccgg ccaggtgcta aagccattgc tcaacggtcg cctcacccgc aacggcaacg
     1981 gcgtccgcgg cgaagacaaa aaaatcgcgt gatatactcc gccgcgctct ggtcagcaaa
     2041 caacgccgag ggtatgactc accatgtcaa tctatgacga tctcggccca ctgagtctgg
     2101 aaaacgtcag tacgtacgct ctcgcaggtc gtcccggcaa agtttcgttg aaggatttcg
     2161 cgcacccgat tgagcaaggg agctcactta gcgacttcct tcacggcctc cccaacattc
     2221 tcgccgtaca aaatctccgg gaactcggtg tgcagatacg gcgcgcccgc gatctaggga
     2281 aaccgatcat ctggggtatc ggcggacacg tgatcaaaac cggactcgca cctctcttga
     2341 ttcagttaat gcagcaagga ttcgtcaccg cgatcgctgc gaatggctcc gtgatcgtgc
     2401 acgactccga aatcgcgcta accggctcga cctcggaaga cgttgatgcc accctcggtg
     2461 agggggtatt tggcggcgct gaggaaacgg gaaagcttct gaaccgcgcc gctggggaag
     2521 gcgcccgtga caccatcggc ttaggcgagg ctgttggtcg tgccttagtg ggcttgaatc
     2581 cgcagcatca aaacgtttcg ctgttgtgtg ctgcttacaa cgccaggctg ccggtaacaa
     2641 tccatctgac gattggtgga gatatcgttc acttccatcc gcaagcaaat ggagctgagc
     2701 tgggcgcgac atcacacacc gatttccgct tgcttgccga gctggtgcgc cgcatgaacg
     2761 gcggcggcgt ttttctgaac atcggatcag cagtagtgct tccggaagta ttcttgaaat
     2821 gcattacgct ggtcaggaat ctgggacatg aactatcgga cataacgact gcgaattttg
     2881 atttcattca gtcttaccga cctctgacga acgttgttcg tcgtcccaca gccgctggtg
     2941 ccgggcgcgg ctattcgatc atcggccatc acgaattaac cctcccactt cttagtgctg
     3001 aactaatctg ctctaacggt tcctcgtagt tctccgttac tcctcttctt aagccttcct
     3061 tcaaatttga cagccgcgac ggaaaggaac catactgaaa acggttttca atttcagccc
     3121 gttggcttac ctggcgtttt catagaaatg aggagttatg ttgcacgcga ttctttggca
     3181 ttcaattaaa gccctccttt tagtaacgat cggcgtgttg ctggctt
//
LOCUS       DASUEX010000280         3868 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_500790, whole genome shotgun sequence.
ACCESSION   DASUEX010000280 DASUEX010000000
VERSION     DASUEX010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3868
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500790"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3794)
                     /locus_tag="VFV61_04730"
     CDS             complement(<1..3794)
                     /locus_tag="VFV61_04730"
                     /inference="COORDINATES: protein motif:HMM:NF014869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HEX4899814.1"
                     /translation="MDASGSGNSKGQTAAPAQAKAAPRASAALEKLRLRATSVVDSHV
                     ARETRHFDFVRAKDGGVLTTDNSSASPEQRALSFLSEHGGLIGMSETEKALAANTDAK
                     SARGASTFKASKTFKDSIGGEHVRLNQTYEGLNVFGAQVVVHMNDRGITAVSGQFVPD
                     IKISTAPAIKESAAEAKALSEQLGGGSLSVVKTDLSIYSKGLLAGYPGPSVLAYNVEV
                     SDGQSMRAQIWIDAMKGTVLDRIPLTHRALNRIIYTPDYDPMFAVRHEGDPLVGPLPA
                     GTTGANPIDNLYVMAGQEYNLFSSAFGRDSFDGLGGTMESVLLVNNQCPNAYWNGVTT
                     NYCPDFDADDVVAHEWSHAYTQYTHGLIYAYQAGGLNEAYSDIFGETVDLLNGVDAEG
                     GSNNNDYMPDGQRWQVGEDVPSINQPALGFLRDMWDAPRYGNPDKVSSPNYDCAADAV
                     HNTSGVPDHAYAILVDGTSRKPDGKFNGQVVQPIGFTRAAHIYFRAMTVYQTPGTSFA
                     MHEQALLASCNDLIGQPLNALSTDNTNHVVSPDVITASTCQQVAKAMLAVEMSADINA
                     RCNYRPILDQNTPLDCPGPTSIFADDFETGTDGWTKTSTGVSPDWEDGTRNLRDFNVD
                     STLPAGRAGSAIQAINAALGEPGGGTCQPGGDYSGQFTIDSPTITIPAQADTLKLSFD
                     HYVATEATFDGGQVEISINGGAYQLLPQEAYEFNAPNSAFLGAVDGNTNPNAGEFAWN
                     GTDQGSFVGSWGTTIANLSSLTNPGDTIKLRFTFSQDGCNGIDGWYVDNVRVYYCPVL
                     EAPVLSLGADYENPDTNGSYTLNWTRPATAVGPDLLQTATVSCAPLLMDEAEGGTGQW
                     SITTAGTVTPGLAWEAVANEKPQHTGTVFRARGTNAVANQASFLTLNTPINIPATGTT
                     FLTFSDWNVNEGDDSVLVEVSTNGTDWTAVYQDIRSELAPDGAAAFATEPFFNRSVNL
                     TGFAAQSIRIRFHYQMGAEDRAGSAPLGWYIDDIAVNNDSWTNVATVAGTSYLASNVS
                     NGTRCYRVRTTYNLGGQTVAGPFSNVVSATVQRTVVEPACFEDDDSHISYDSGWHSVN
                     YADASGGHFRLGTGNDGQHGMTLNFDVTGGQTGKITYNYLKSTKGGTADVYLDGTFRQ
                     TINYKGSVGKLREPEKQAAYQVSFSGLAAGGHVLELRNMRGAVYVDGFCMESSSSSAQ
                     PSAGPGTTSSNTTSLNLGKQLLQSLTLPAGTQAISVVAEASPELPIQLLLIDPSGAVL
                     NTA"
BASE COUNT          796 a         1092 c         1092 g          888 t
ORIGIN      
        1 gctgtattca acaccgcccc ggaaggatcg atcagcaaga gctgaatcgg taactccgga
       61 ctggcctcag ccaccactga gatcgcctgg gtgccggcag gtaaggtcag gctttgtagc
      121 aactgcttgc caagattcaa cgaagtagtg ttgctactgg ttgttcctgg acctgcgctc
      181 ggttgcgcgc tcgagctcga gctctccatg cagaagccgt ccacatagac cgcgccacgc
      241 atgttccgta gctcgaggac gtggccccct gccgccaggc cgctaaacga aacctgataa
      301 gcggcttgct tctcgggctc acgcagtttg ccaacgctgc ctttgtaatt gatggtttgt
      361 ctgaacgtgc catcaagata gacgtcagca gtgccgccct tagtgctctt aagatagttg
      421 tacgtgatct ttcctgtctg cccaccagtc acatcaaagt tcagcgtcat tccatgctgg
      481 ccgtcattgc ctgtacccag ccggaagtgc ccgccgctcg catccgcgta gttaaccgaa
      541 tgccagccac tgtcgtagct gatatgactg tcgtcatcct caaagcaggc tggttcgact
      601 acggtgcgct gcactgtcgc cgagaccacg ttgctgaacg gtcccgcaac ggtttgtcca
      661 ccgaggttgt aagttgtgcg cacgcgatag cagcgcgtgc cattactgac gttcgatgcg
      721 agataggaag tgcccgccac cgttgccacg ttggtccagc tatcgttgtt cacagcgatg
      781 tcgtcgatgt accaaccgag tggcgccgaa cccgctcgat cctccgcacc catctgataa
      841 tggaagcgga tgcgaatcga ttgcgcagcg aatcctgtca ggttcaccga gcgattgaag
      901 aacggttccg tagcaaatgc tgccgcccca tcaggcgcga gttcggagcg gatgtcctga
      961 taaaccgcag tccaatccgt gccattggta gaaacttcaa cgagtaccga atcgtcgcct
     1021 tcattcacat tccaatcaga gaaagtaagg aacgtcgttc ccgttgccgg aatgttaatc
     1081 ggagtattca gcgtcaggaa cgatgcctga ttagctacgg cgtttgtgcc gcgcgcacgg
     1141 aaaacggttc cggtatgttg cggtttctca ttagctaccg cttcccacgc caagcctgga
     1201 gtgactgttc ccgcggtcgt gatgctccac tgaccggttc caccttcggc ttcatccatt
     1261 aacagtggag cgcaagacac cgtcgccgtt tgcaacagat ctgggccgac cgcggtagcg
     1321 ggccgcgtcc agttaagtgt gtacgagcca ttagtgtcgg gattctcata gtccgcgccc
     1381 aaagaaagaa caggcgcttc gagaaccgga cagtagtaga cgcggacgtt gtcgacgtac
     1441 cagccgtcga tgccgttgca accgtcctgg ctaaaggtga accgaagctt gatcgtatcg
     1501 cctgggtttg tcagcgaaga aagattggcg atggttgttc cccaggagcc tacgaacgag
     1561 ccctgatcag taccgttcca cgcaaactcg cccgcgttgg gattggtgtt accgtcaacg
     1621 gcacctagga atgcgctgtt cggcgcgttg aattcataag cttcctgtgg caagagttga
     1681 tacgctccgc cgttgatact gatctccacc tggccgccat caaacgttgc ttcagttgcc
     1741 acgtagtgat cgaaactcaa tttgagcgtg tcggcctggg ccggaatggt gatcgtcggg
     1801 ctgtcgatcg tgaactggcc cgaatagtcg ccgccgggct gacaggttcc gccgccaggt
     1861 tctcccaggg cagcgttgat cgcctgaatt gccgagcctg cacgtcctgc cggcaaggta
     1921 gaatcgacgt tgaaatccct gagattgcgc gtcccatctt cccaatccgg cgacacaccc
     1981 gtgctggttt tcgtccaacc atccgtgccg gtctcgaaat cgtccgcgaa aatactggta
     2041 ggcccaggac agtctagcgg cgtgttctga tcgagaattg gccggtagtt gcagcgcgcg
     2101 ttgatgtcgg cgctcatttc cactgccagc atggccttag cgacttgctg gcaagtgctc
     2161 gcagtgatca catcaggcga gacaacgtgg ttagtgttgt cggttgataa ggcattcagc
     2221 ggttggccga tcaaatcatt acacgacgcc aatagggcct gttcgtgcat ggcaaagctg
     2281 gttccgggtg tttgatagac tgtcatcgcc cggaagtaaa tgtgcgcggc gcgagtgaag
     2341 ccaatcggct gaacgacctg cccgttgaac ttgccatccg gttttctgga agtgccatct
     2401 acgagaatgg cgtaagcatg atccgggacc ccggatgtat tgtgcaccgc atcggcggcg
     2461 caatcgtaat ttggcgaaga caccttatcg ggattgccat aacgcggcgc atcccacatg
     2521 tcgcgcagga aaccgagggc gggttgattg atggacggaa cgtcctcacc aacctgccag
     2581 cgctgcccgt ctggcatata gtcgttgttg ttagagccac cttcggcatc gactccgtta
     2641 agcaggtcaa ctgtttcgcc gaagatatct gaataggctt cgttcaaacc gccggcttga
     2701 tacgcgtaga tcaacccgtg cgtgtattgc gtgtaggcat gtgaccattc gtgtgcaacc
     2761 acatcgtcgg catcaaagtc gggacagtaa ttggtggtca caccgttcca gtaagcattg
     2821 ggacactggt tgttaaccaa aagaacgctc tccatcgtgc cgcccaggcc gtcaaacgaa
     2881 tcgcggccaa aagccgacga gaagaggttg tactcctgtc cggccatcac gtagaggtta
     2941 tctattggat ttgcccccgt ggtgcctgct ggaagtgggc ccactaatgg gtcaccttcg
     3001 tggcgcaccg caaacatcgg atcgtaatcg ggcgtataga tgattcggtt cagcgcacgg
     3061 tgagttagcg gaatccgatc gaggactgtg cctttcatcg catcgatcca gatttgtgcg
     3121 cgcatgcttt gtccgtccga aacttcgacg ttgtaagcca gcacgcttgg gcctggatag
     3181 ccggcgagca agcccttgct atagatgctc aaatccgtct ttacgacact gagcgaaccg
     3241 ccccccagtt gttcgctcag tgccttcgcc tctgccgcgg attctttgat cgccggggcc
     3301 gtgctgattt tgatgtcggg aacaaactgg ccgcttacag ccgtgatgcc gcggtcgttc
     3361 atgtgcacga ctacctgcgc accgaacacg tttagtcctt cgtaggtttg attgagtcgg
     3421 acatgctcgc cgccgattga gtctttgaaa gtcttgctgg ctttgaaggt actcgcgccg
     3481 cgggcactct tggcgtctgt gttggctgcc aaagcttttt cggtctcgct catgccgatg
     3541 agcccgccgt gctcactcag gaatgagagt gcgcgctgct ccggcgaagc agaagagttg
     3601 tcggtagtta agacaccgcc atcctttgcg cgtacgaagt caaagtgccg ggtctcgcgg
     3661 gcaacgtgtg aatcgaccac cgaggtggct cgcagtcgta atttctcgag tgcagcacta
     3721 gctcttggtg cagccttggc ctgggccggt gctgccgttt ggcctttgct gtttcctgat
     3781 ccggaagcgt ccacgctttc agaaatgcga aacgcgctca agccgagcgc ggtcaaacag
     3841 aaaataagag caagtgaaaa tcttaaat
//
LOCUS       DASUEX010000281         2892 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_56821, whole genome shotgun sequence.
ACCESSION   DASUEX010000281 DASUEX010000000
VERSION     DASUEX010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2892
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56821"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..963
                     /locus_tag="VFV61_04735"
     CDS             <1..963
                     /locus_tag="VFV61_04735"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4899815.1"
                     /translation="GCGKTTTLRMIAGLEIPDSGTIQFADREITLLSPEKRGFGMVFQ
                     NYALFPHLNVFENVAFGLRARHKPKAEVVSAVSSALELVQLPGYEKRRIDELSGGQQQ
                     RVAIARAIAIEPALLLFDEPLSNLDVALREETRSELRELVNRLGLTAVYVTHDQEEAF
                     ALCDRISVMMGGRILQTGAPRELYEHPAQISVARFLGRNNLIQVMRLSSSKATVAEFK
                     TLAGGHTLQLPAAHDELAPLNKPCSLAIRPEHLKLSQTNPDRPNVVPARISEINFAGA
                     TSSIKLDANGLHLDALVLQPDGFSAGDQCFVELPSDRLSLLTEG"
     gene            1162..>2892
                     /locus_tag="VFV61_04740"
     CDS             1162..>2892
                     /locus_tag="VFV61_04740"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX4899816.1"
                     /translation="MTKHPLPGDNRVIPSSRFWVAAPGRLILLLITAVVLLYGVVYPN
                     AHTVLSSLQIDGQWSLTTYREVLTQGIVIESVFTSLGVSLLTVLCCALVGVPLAFLFE
                     RYTFPLRRLFAALAALPLVLPPLVGTVAFILLCGESGILARAVQTALHFETPPWTLRG
                     WSALLLFHTYTMYPFFYVLTGAGLRRIDAGLAEAAKGLGANPVYVLLRVTLPQLTPSL
                     LAAGLLTFMVSMASFSAPLLFGGNVRMLTLEIFTARQRGDTRVALTETVILAMISLSA
                     LIFFQRYEGTRKFAAAALKGSSRRLRPITSGKARLAAMVCGIAFSVLLILPVATLALV
                     SFARDGIWTTQTLPPAYTLGNYTRLLTEQNASEVFLNSLAMAGIASVAALLWSFVVAT
                     LVTAKTRSGPRRLLSLLVLVPWALPGTVVAISIAEAYGKPGPLLGSYILVGTFWILPV
                     VYFLRFMPLVVRAVQASMEQLDPALNEAAGSLGAKWWQRFLRITLPLVWPGAVAGTLL
                     AFVIALGEYVASVLVFVPTNRPISIAIAAELRDFNLGSAAAYGMLLILPVATLALVSF
                     ARDGIWTTQTL"
BASE COUNT          555 a          828 c          797 g          712 t
ORIGIN      
        1 ggctgtggca agacgacgac gctgagaatg atcgcgggtc tggaaatacc cgactccggc
       61 accatccagt ttgctgatcg cgagatcacg ctcctctcac cagaaaaacg cggcttcggc
      121 atggtctttc aaaactacgc actcttccct cacttaaacg tcttcgaaaa cgtcgccttc
      181 ggactccggg cgcgccataa accaaaagcc gaagtagtta gtgcggtcag cagcgccctg
      241 gaactggtcc agcttccggg ttacgagaag agaagaatcg atgagctctc gggcggccaa
      301 cagcaacgcg tggcgattgc ccgcgcgatt gcgatcgagc cggccctgct tctgtttgat
      361 gagccccttt caaatctcga tgtggccttg cgcgaggaga ctcgcagcga gttgcgcgag
      421 cttgttaatc gtttgggatt gacggccgtt tatgtaacac acgatcagga ggaggcgttt
      481 gcgctctgtg atcgcatcag cgtgatgatg ggcggtcgta tcctgcaaac tggtgccccg
      541 cgcgagttgt acgaacatcc ggcgcagatt tcggtcgcac gcttcctggg acggaacaac
      601 ctgattcaag taatgcgctt gagttccagc aaggccacgg tcgcagaatt caagacactc
      661 gcaggaggtc acacgctgca actcccggcg gcacatgatg aattagcgcc gctgaataag
      721 ccctgctcac tggcgattcg tccggaacat ctgaaactga gccagacaaa cccggaccgg
      781 ccgaatgtgg tgccggcccg gatcagcgaa atcaatttcg ccggcgcgac ttcgagcatc
      841 aaactcgatg caaacggtct gcatcttgat gcactcgtgc tacagccgga cgggttttcg
      901 gcaggagacc aatgcttcgt cgaattaccc tccgaccgac tctcactctt aactgaggga
      961 tgagggataa gatcattgtc aacggagagc tcaattcaga aggtaacgct cacttaggaa
     1021 ggtgttgccc ggtctcaccc cgataggggt gaaattcaat agccgtgggc aacgcccacg
     1081 gaacaggttc gttaagccac ctaaaccgtg ggcgttgccc acggctactg actctcacgc
     1141 cttcggcgtg aagcagcgtt gatgaccaag catccattac ctggtgacaa ccgcgttatt
     1201 ccctcatctc gcttctgggt tgcggcgccg gggcgtctca tactcctcct aatcaccgct
     1261 gttgtcctgc tctacggagt cgtctatcca aacgctcaca ccgttttgag ttctctgcag
     1321 attgacggcc aatggtcgct cacaacttat cgcgaggtcc ttactcaggg catcgtgatc
     1381 gaatcggtgt tcaccagtct gggagtctcg ctgcttacgg ttctctgctg cgcgttggtc
     1441 ggcgtgcccc tggcgttcct cttcgagcgc tatacctttc ccttgcgcag actgtttgca
     1501 gctctcgccg ccttgccgct ggtgttacct ccattagtag gcaccgttgc tttcattttg
     1561 ctttgcggtg agtcagggat actcgcgcga gcggtgcaga ccgctcttca cttcgaaacg
     1621 ccgccctgga cgctacgagg ctggtcagcg ttactcctgt ttcataccta caccatgtat
     1681 ccgtttttct atgtgcttac aggcgcagga ttgcggcgca ttgatgctgg acttgctgag
     1741 gctgcgaagg gtttaggagc aaatccagtc tatgttcttt tgcgggttac tcttccacaa
     1801 ctaacaccat cgctactcgc tgccggattg ttaaccttca tggtctcgat ggcatcgttt
     1861 agcgcgccgc tactctttgg cggcaacgtg cgcatgctga cgctcgagat tttcaccgcg
     1921 cggcaacgcg gagacacacg cgtggcgtta acagaaactg tgatcctcgc catgatttct
     1981 ctgagtgcgc tgatcttttt ccagcgttat gaaggaacga gaaaatttgc ggcagccgcg
     2041 cttaagggaa gctcccgccg gctgcgacca attacgagcg gcaaagcacg tttggcagca
     2101 atggtctgcg gcattgcttt ctcagtgctc ctgattctgc ccgttgccac tcttgccctc
     2161 gtgagttttg cgcgtgatgg aatctggacg acgcagacac ttccgcccgc ctacacgctc
     2221 gggaattaca cacgcctcct taccgagcaa aatgccagcg aagtattcct gaacagcctg
     2281 gcaatggccg gcatcgcttc cgtggcggct ctgctttgga gtttcgttgt cgccaccctg
     2341 gtgacggcga agactcggag tggcccgcgg cgtttgcttt ccttgcttgt cttagtccct
     2401 tgggccttgc ctggcactgt cgtcgctatc tcaatcgctg aggcttacgg caaacctgga
     2461 ccactgctcg gttcttatat tctggtaggc acgttttgga tcctgccggt agtttacttt
     2521 cttcgtttca tgccgctcgt cgtccgtgct gtgcaggcga gcatggagca gctcgatccg
     2581 gctctcaacg aagcagcagg aagtttgggg gcgaagtggt ggcaaagatt cttgcgcatt
     2641 accttgcctt tggtttggcc gggagcagtc gcgggtacac tgttggcgtt tgtcatagct
     2701 ttaggcgagt atgtcgcgtc ggtgctcgta ttcgttccca ctaatcgacc gatatcgatc
     2761 gccatcgctg cggagttgcg cgatttcaat cttggttcgg cggcagcata tggaatgctc
     2821 ctgattctgc ccgttgccac tcttgccctc gtgagttttg cgcgtgatgg aatctggacg
     2881 acgcagacac tt
//
LOCUS       DASUEX010000282         2061 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_974048, whole genome shotgun sequence.
ACCESSION   DASUEX010000282 DASUEX010000000
VERSION     DASUEX010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_974048"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..653)
                     /locus_tag="VFV61_04745"
     CDS             complement(87..653)
                     /locus_tag="VFV61_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899817.1"
                     /translation="MKALVLILLMLGIVSFDVKAQRPRAVDNTPTKATAARVAPAPQT
                     VKVKYEGGVFGYKKTMEGTLTFDDTNNRLLFKNKDQKEMFFIPYNAVTSAFADTQKRR
                     PAAATVASAIPLIYALPAQFIKTKVRYLTMNFYDPDTHVSGITSFRMENREILASVLQ
                     SLAEKTGMVARGEVYVRQQPADPNKTNP"
     gene            complement(711..1949)
                     /locus_tag="VFV61_04750"
     CDS             complement(711..1949)
                     /locus_tag="VFV61_04750"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX4899818.1"
                     /translation="MLNQQLLRLISLIALVFTLGGVVAAQSSRPRRVKPAPKPAEDPL
                     LRPEPRTTTTARTDPNAPLIKVQPVQPVANPIASGDTANAYQLLQQKQFAAAAKEAKA
                     VAANFPNDAEAWKIAGFAELNLKQYTEAASDLEKAVALQRAAKQEDPNTVDALAQAYV
                     LSEKFEPALPLLVNATSRAGAKPDVAMLYYRGLAEYKTGKVEAAERTFNAVLKLNPRD
                     TLSLFYLAQIALGRNDLDGAIVALNRATVNDARMASAWTLLTSVYLRRAAAATDTTKA
                     DADYLNAVRAGEGLIKVRTDAEAVTLFGQALIGAQQYARAAAALERAAQGDAPGVTFY
                     LLGVAQSRAKNFPKAIAALETAATKTPNDVNVYRELGYAYEVSKQYGKALGAYQKGAN
                     LAPGDGDFKESIERVRPFAK"
BASE COUNT          413 a          571 c          581 g          496 t
ORIGIN      
        1 ctgagcatga tgtcgcggtg cgctacccac aaaaaaggcg ggctgcctcg cccgcctctc
       61 cttcatttac tcataattcc cgagagctaa ggattcgtct tgttgggatc cgctggttgc
      121 tgccgcacat acacctcgcc acgcgctacc attcccgtct tctctgccag actctgcaac
      181 accgacgcga gtatttccct attctccatg cggaaactgg taatgcccga gacgtgtgtg
      241 tccggatcgt aaaaattcat cgtcaggtag cggacctttg tcttgatgaa ttgggcgggt
      301 aacgcgtaga taagcggaat cgcgctggca actgtcgctg ccgcaggtcg tcgtttctgc
      361 gtgtcggcaa aggccgaagt cacagcattg taaggaatga aaaacatttc cttctggtct
      421 ttgtttttaa acaggagccg gttattggtg tcgtcaaacg ttaaggtgcc ttccatggtt
      481 ttcttatagc caaacacccc gccttcgtat ttcactttca cggtttgtgg cgccggcgcg
      541 accctcgccg ccgtagcctt ggtgggggtg ttatcgaccg cacgcggtct ctgtgctttc
      601 acatcgaacg acacgattcc gagcatgagt aagataagaa ctagggcttt catatgtacc
      661 tcccgaggat atgccttacc gggttccggt gtcaggtgcc ggttgtcgaa ctatttagca
      721 aacgggcgca cccgttcaat cgattctttg aagtcgccat cgcctggcgc caggttcgcg
      781 cctttctgat aggcgcctaa cgccttgcca tactgctttg agacttcgta ggcgtagccg
      841 agttcacgat agacgttaac atcattcggc gttttggtcg ctgctgtttc aagcgcggca
      901 attgctttag gaaagttttt ggcgcgcgac tgagccacac ccaacaggta aaacgtcacg
      961 cccggcgcgt cgccctgggc cgcacgctcg agtgctgctg ctgcgcgcgc atattgctgg
     1021 gcaccaatca acgcctgtcc gaaaagagtc acagcttcgg catcggtgcg gactttgatg
     1081 agtccttcac cggcgcgcac agcattcaaa taatcggcgt cggccttcgt cgtgtcggtt
     1141 gccgccgccg cgcgccgcag atataccgag gtcaacaacg tccacgctga ggccatgcgt
     1201 gcgtcgttca cagtggcgcg attaagagcg acgatcgccc catccaggtc gttgcggccg
     1261 agagcaattt gcgcgagata gaagagtgaa agcgtgtcgc gcggattgag tttgagaacg
     1321 gcgttgaagg tacgctctgc agcttccact ttgccggtct tgtattcggc gagtccacga
     1381 tagtagagca tcgccacatc aggtttggct cccgctcgtg aagtcgcatt gaccagcaac
     1441 ggcaaagcag gctcaaactt ttcggaaagc acataagcct gagcaagcgc gtcaaccgtg
     1501 ttgggatctt cctgtttcgc agctcgctgt agcgcgacag ctttttccag atcactcgcg
     1561 gcttccgtgt attgcttcag attcaattca gcaaagccgg cgatcttcca ggcttccgcg
     1621 tcattcggaa aattagcggc gactgccttg gcttctttgg ccgccgccgc aaattgtttc
     1681 tgctggagca actgataggc gtttgccgtg tcgccggaag cgattggatt ggcgaccggt
     1741 tggaccggct gcaccttgat caaaggggca ttcggatcag tgcgcgccgt ggttgtggtg
     1801 cgcggttccg ggcgcaaaag cggatcttcg gcaggcttgg gggccggctt tacgcggcgc
     1861 ggccggctcg attgcgccgc gacaactccg ccaagtgtaa acaccagagc aataagggaa
     1921 atcagccgaa gtaattgttg gttaagcata ggatttcttc cggggagagg attgttgcgg
     1981 cgaataaaga tgctggagca acagctttcg gatgccaacg ttctctcgat agttcgccta
     2041 ccggcaattt agctcaactc c
//
LOCUS       DASUEX010000283         3062 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_57161, whole genome shotgun sequence.
ACCESSION   DASUEX010000283 DASUEX010000000
VERSION     DASUEX010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3062
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57161"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2800)
                     /locus_tag="VFV61_04755"
     CDS             complement(<1..2800)
                     /locus_tag="VFV61_04755"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX4899819.1"
                     /translation="MTSKQPDNRKFVGALLHDMKLSPTEIFQNRNIFLIGGTGFLGKV
                     TLSMLLHRFPNVGRVYVSVRARSQEESETRFWNSVITAPPFDPLRARYGAALEGFIRS
                     KVVVLGGDIGDTNLGYSEEEAQRVADDIDVIINSAGNVTFNPTLESALRTNVVGTQNV
                     IAFAKRMKRPALVHVSTCFVAGNRQGTIWESDPVLGYFPRKNELSGVEFSVEQEIKDC
                     ARLAERVKEDAKDAMMVARFRELARRRLIEEGRDEDDPDALGLAVARERKVWTRTRLT
                     DLGVERSAWWGWPNIYTYTKALGEQLVAAETGIVRGIVRPSIVESALSYPFPGWNEGF
                     TTTAPIIFLVLKGQTQIPANDKLILDITPVDQVASVMLAVAAQACVEEPRLVYQAATG
                     DANPATMERIISLVGLYKRKHFKEKETGIKLLNEAAARMAPRPVSAARFEATSIPMFN
                     SAAKKASALLDRARPRWGGGRMVEVIDRVKTSVDRVEEVTRETTDSFEIFRPFTIENA
                     YLFRSDNVRSLFERIRKDEQSLLTWNPEKFDWYTYWINVHIPGLKKWVFPKLEDDMRA
                     QPKRTYTYRDLLELFETSTKRHATRVAMRIERDERTEQYTYADLNELATRAAAFLASE
                     GVKPGDRVMLFSHNAPEWGMTYFGVLKTGASCIPVDPESKLGEIVNFARAGDAAGIVI
                     SERLDREHPELRQRLSDEGLSLRVWTFPEVFALQDETTEDQRIALLPQKVHAQSVASL
                     IFTSGTTGRPKGVMLSHRNLTSMVSMLSSVFDMSTKDGVLSVLPLHHTFEFSTGFLTP
                     LSRGAQITYLPELTSDALARAIKNGHVTGMVGVPALWELLHRRIKNRLYERSDWIGKT
                     ADALIKANFWLREKTPLNLGPIVFYPIHEGLGGRIRYFISGGSALSEKIHQDFQGLGF
                     TILEGYGLTEASPVLTVTR"
     gene            complement(2832..>3062)
                     /locus_tag="VFV61_04760"
     CDS             complement(2832..>3062)
                     /locus_tag="VFV61_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899820.1"
                     /translation="YHGAHPFFMWYWGENGRQHVGKVIAAGAENAHVPAMLGWERADN
                     LTEAIAMARSYMGNSAEITMLHQPMIGIADME"
BASE COUNT          656 a          875 c          845 g          686 t
ORIGIN      
        1 gacgcgtgac tgtcagcacg ggagatgctt cggtcaggcc atagccctcg agaatcgtga
       61 agcccagacc ctgaaagtcc tgatgaatct tttcgcttaa cgccgagccg ccgcttatga
      121 agtatctaat tcggccgccc agtccttcat gaattgggta gaagactatc gggccaaggt
      181 taaggggcgt tttttcgcgc agccagaaat tggccttaat gagtgcatcg gcagtcttgc
      241 cgatccaatc actacgctcg tagagccgat tcttgatacg ccggtgcagc agttcccaaa
      301 gcgccggtac ccctaccatt ccggttacat gaccattctt gattgcgcgg gcgagggcat
      361 cacttgtcag ctcaggcagg tatgtgattt gtgcaccgcg actcagcgga gtcaggaacc
      421 cggtggaaaa ctcgaatgtg tgatggagtg gtaagaccga aagcaccccg tctttggtgg
      481 acatatcgaa gacggaggaa agcatggaga ccatgctcgt caggttgcga tgcgaaagca
      541 tcaccccttt gggccggccc gtggtaccgg aagtaaaaat caaagaagcg acagattgag
      601 cgtggacttt ctgcggcagg agggcgatgc gttgatcctc agtggtctcg tcctgcaggg
      661 caaacacctc gggaaaagtc cacacacgca ggctcaatcc ttcatcgctc aaccgctgtc
      721 gcagctccgg gtgttcccgg tcaagtctct cgctaatcac gatgccggcc gcgtcgcccg
      781 cgcgcgcgaa gttgacgatc tcgcccagct tgctttcggg gtcaaccgga atacagctgg
      841 caccggtttt gagcacgcca aagtaagtca tgccccactc cggcgcgtta tgactaaaga
      901 gcatcacacg atcgccgggc ttcacgcctt cgctggcgag gaaagctgcc gcgcgcgttg
      961 cgagttcatt cagatcggcg taggtgtact gctcagtgcg ctcatcacgt tcgatgcgca
     1021 tggcgactcg ggtcgcatgc cgcttggtag acgtctcgaa gagttcaagc agatcccgat
     1081 aggtgtaggt gcgcttcggt tgcgcccgca tatcatcttc aagtttcgga aagacccact
     1141 tcttcagccc gggaatatgg acgttgatcc agtaggtgta ccagtcaaac ttctccggat
     1201 tccacgtcaa cagagactgc tcatctttgc gaatgcgctc gaaaagggaa cgaacattgt
     1261 ccgaacggaa caagtaggca ttctcgatcg tgaacggccg gaagatctca aaagaatctg
     1321 tggtctcgcg cgtcacttct tcgacacgat cgaccgaagt cttcacacga tcgattactt
     1381 ccaccattcg cccgccgccc cagcgtggac gggcacggtc gagcaacgcc gaagctttct
     1441 tcgccgccga gttaaacatc ggtatcgatg tcgcttcaaa gcgggccgct gagactggtc
     1501 ggggcgccat ccgcgccgca gcttcgttga gcagttttat cccagtctct ttttccttga
     1561 agtgctttct cttgtagaga ccgacgagag agatgatccg ctccatcgtg gcagggttcg
     1621 cgtcgcccgt cgcggcctgg tagacgagcc ttggctcttc gacgcatgcc tgggcggcga
     1681 ctgccaacat cactgacgcg acttgatcca ccggggtgat gtccaggatc aacttgtcgt
     1741 tggcgggaat ctgggtttgt cctttcaata ccagaaagat tatgggcgcc gtagtggtaa
     1801 agccttcgtt ccaacccggg aagggatagg aaagagcaga ctccacgata ctgggccgca
     1861 cgatgccgcg gactattcct gtttctgcgg ccacgagctg ctcgccgagg gctttggtgt
     1921 aggtataaat gttaggccaa ccccaccaag ccgagcgctc cacgccgaga tccgtcagtc
     1981 gcgttcgcgt ccagaccttt cgctcgcggg cgaccgctag tcccaaagcg tcaggatcat
     2041 cttcatcgcg tccttcttca atcaagcgcc ggcgcgcgag ttcgcgaaag cgagccacca
     2101 tcatcgcgtc tttcgcgtct tccttcactc gttcggccaa acgggcacag tctttgattt
     2161 cctgttcaac tgaaaactcg accccggaaa gctcattctt gcgcgggaag taacccaaca
     2221 ccggatcact ttcccaaatc gttccctgac ggttacccgc cacgaaacac gtagaaacgt
     2281 gaacgagcgc gggacgcttc atgcgcttgg caaaagcgat aacattctga gtgccgacga
     2341 cgttggtgcg gagagcgctt tcgagagtgg gattgaaagt gacgttaccg gcgctgttga
     2401 taatgacgtc gatatcgtcg gcgactcgct gggcttcctc ttcgctgtat ccgagattcg
     2461 tatcgccgat gtcgccgccg agcaccacaa ccttactgcg aatgaaacct tcgagcgcgg
     2521 cgccgtagcg tgcgcgcaag ggatcgaatg gtggcgcggt gatcacgctg ttccagaagc
     2581 gcgtttccga ttcctcttgt gaacgggcac gcacagaaac atacacgcga ccgacattgg
     2641 gaaaacgatg caacagcatg ctgagcgtca ctttccccaa aaatcccgtg ccgccaatca
     2701 gaaagatatt gcggttttga aagatttctg tcggcgacaa tttcatgtcg tgcagcagtg
     2761 cgccgacgaa ctttcgatta tctggttgct ttgaagtcat ctaactcgtt tatagcttct
     2821 gattcgaact cttattccat gtctgcgatg ccaatcattg gttgatgcag cattgtgatt
     2881 tcggccgagt tgcccatgta actgcgggcc atggcaattg cctcagttag attatccgca
     2941 cgttcccaac cgagcatcgc cggcacgtgg gcgttttcgg cgcccgcggc gatgactttg
     3001 ccgacgtgct gacgcccgtt ttcaccccag taccacatga agaacggatg ggctccgtga
     3061 ta
//
LOCUS       DASUEX010000284         4471 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1295676, whole genome shotgun sequence.
ACCESSION   DASUEX010000284 DASUEX010000000
VERSION     DASUEX010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4471
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1295676"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2891)
                     /locus_tag="VFV61_04765"
     CDS             complement(<1..2891)
                     /locus_tag="VFV61_04765"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX4899821.1"
                     /translation="MNLSEPFIRRPVMTAVLTLSVILFGVLSYFQLPVNDLPAVDYPV
                     IQVVVGYPGASPDTIANNIATPLERQFMQINGLEVVTSQSTQGVAILTLQFALEKNID
                     AAATDVQTAISQAIGNLPADLPSPPTYSKSNPNDQALMYIALTSDSVTTGQLYDYAST
                     QVGQRLSILPGVSQVNVFGSKSAIRIKANPSAMWARGISIDDLNAAIKSGTSYTGAGQ
                     LDSASSTAILNPKGQLETPEQYANLIVGGTNNAPIYLRDVATIEQSLQDERVNMRFWV
                     RGYPVPSATVILAVNRRAGANAVEVSRSIHSVLPMIAAELPGSIRVTPIYDRSQNIVH
                     SVTDVRMTLIIAFMLVVIVIFAFLGRVTDTLIPVVALPLSLLITFIAMRALGYSLDNL
                     SLMALTLAIGFLVDDAIVFLENTVRRMERGEQPFTAAINSAREISFTIMSMTISLAAV
                     FIPLVFMGGLVGRIFREFAVTIVIAILASGLVSLTLTPLMCARLLKGRGEGSRKTWVE
                     RVIGGIEKRVLALYGGSLWWFLRHRWISPVIWILCLAGTIVLFIFVPKSFLPPGDSSV
                     IFGAFIAREGSSPKQMQEYQTRVDEVLQSDPHVVTEFTLTGATGFLASNQGITFTLIN
                     PPEERRPIAEVTAELMGKLNTIPGLMTFLRPFPVLEISTGVTSQNQGQYAFTVSGANP
                     QQVYDVGQKLMGKLMEYPGFLSVSSDFYNNTPNLSIDIRRDQAKTYGVSEARILTLLR
                     NAYSQNYLYLIKKPDDQYQVILEMEDAARENPEDLSLLYIKTDDGQRLVPLKELVTWK
                     QTLGPQTVNHLNQFTSMSLFFNLKPGVALGDATNFINQSAREIVPPGVRAELQGEAQT
                     FSNTVTSLTVLMALAVFVMYVILAILYESYVHPLTVLSTLPTALVGGLLTLLVFGQEA
                     SLYAFVGMFMLMGIVKKNGIMIVDFARHRVEAGEAADKAIHDASMDRFRP"
     gene            complement(2888..4150)
                     /locus_tag="VFV61_04770"
     CDS             complement(2888..4150)
                     /locus_tag="VFV61_04770"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX4899822.1"
                     /translation="MLYLPKVSLLAISFSLLLSGCANKAQQSFDRPPSPVQVSVAVSQ
                     DVPTYIDALGKTVAREVVSIQPQVSGRITKIHFTDGANVRKGDLLFTIDTRPFEASLR
                     QAQANLSKDGALKKQAEANLAKEIAQANWGLAQVERYRTLVQQGVVPREQYEELRANY
                     DSLKANVEAARAAVRSADEEIKVDAAAVENAKVNLSYCYIHSPIDGRAGQRLVDLGNV
                     VNPGGSSNNNGDGSSSNTNALLVIERLDPIYADFTISQASLTAVQQQMRAGALKVEVH
                     LPDMVDEMVAGQLTFLDNAVQTTTGQVTLRATVPNSDHRFWPGRFVNIRLVLNTIHSA
                     VLVPASAPQMSAKGSFVYVVKQDSTAEQRPVSLGQRQGDLIVVESGVAAGEQVVINGQ
                     LGVTPGGKVLIEQPREAGAAAANSGGSK"
BASE COUNT         1002 a         1222 c         1254 g          993 t
ORIGIN      
        1 ggccggaagc gatccatact cgcatcgtgg atcgctttgt ctgctgcctc acctgcctca
       61 actcggtgac gcgcaaagtc gacgatcatg attccatttt tcttcacgat gcccatcaac
      121 ataaacatgc cgacaaacgc atagagcgaa gcttcctgac caaatacaag caacgtgagt
      181 aatcctccaa ccaacgccgt cggcagggtc gacaacacgg tgagcgggtg cacatagctc
      241 tcgtacagga ttgccaaaat cacatacatc acgaagacgg cgagcgccat cagcactgtc
      301 aaactcgtga cggtgttgct gaacgtctgt gcttcaccct gcaactccgc gcggacgcca
      361 ggcggcacaa tctcgcgcgc cgactgattg atgaaatttg tcgcatcacc gagcgcgacg
      421 ccgggtttga gattgaagaa gagcgacatg ctggtgaact gattcaggtg gttcactgtc
      481 tgcggcccca gcgtttgttt ccaggttacc aattccttga gcggcaccag ccgttgtccg
      541 tcatcggttt tgatatagag cagcgacaga tcttccggat tctcacgcgc agcgtcttcc
      601 atctccagaa tcacctggta ttgatcgtcc ggcttcttga tcaggtagag atagttttgc
      661 gagtaagcgt tgcggaggag cgtgagaatt ctcgcctcag agacgccata cgtcttcgcc
      721 tggtcgcgac gaatgtcgat gctcaggttg ggcgtgttgt tgtaaaagtc tgacgagacg
      781 ctaagaaagc ccgggtattc catcagcttc cccatcagct tttgccccac atcgtagacc
      841 tgttggggat tcgctcccga gactgtaaac gcgtactgtc cctggttctg actcgtaact
      901 ccggtgctga tttccaaaac cggaaacgga cgcaggaatg tcatcaagcc gggaatggta
      961 ttgagttttc ccatcaactc ggcggtgact tccgcaatag gccggcgttc ttcgggtggg
     1021 ttaatcagtg tgaaagtgat tccctgattg gaagccagaa acccggtagc tcccgtcagc
     1081 gtgaactcag taacgacgtg cggatcgctt tgtaagactt cgtcaacccg agtctgatac
     1141 tcctgcattt gtttcggcga agagccttcg cgcgcaatga aggcgccaaa aataacgctg
     1201 ctgtcacccg gcggtaagaa cgacttcggc acgaagatga acaggacaat cgtaccggcg
     1261 agacagagaa tccagatcac cggggagatc cagcgatgac gcaagaacca ccagagcgag
     1321 ccgccataca gcgcgagcac acgtttctcg atgccgccga tcacgcgctc tacccaggtc
     1381 ttccttgaac cttcgccgcg tccttttagc aagcgcgcgc acatgagcgg ggtcagtgtt
     1441 aacgaaacta aaccagaagc gagaatcgcg atgacgatcg tgacggcaaa ctcacgaaag
     1501 atgcgcccga caagcccgcc catgaacacg agcggaataa atactgccgc caacgaaatc
     1561 gtcatcgaca tgatcgtgaa actgatttcc ctggcactgt tgatggcagc agtgaaaggt
     1621 tgttcgccgc gctccatgcg gcgcacggtg ttctccaaaa agacgatcgc gtcgtcgacc
     1681 aggaatccaa tcgccagcgt caaagccatt aacgagaggt tgtcgagact gtacccgagc
     1741 gcgcgcatgg cgatgaaagt gatcagcaac gagagcggca gggcgactac tggaatgagc
     1801 gtgtcagtga cgcgaccgag aaaagcgaag atcacgatca ctacgagcat gaaggcgatg
     1861 atcaaagtca tccgcacgtc ggtaaccgag tgcacaatgt tctgcgagcg gtcataaatg
     1921 ggcgtaacgc gaattgaacc gggtagctcc gcagcgatca ttggaagaac actgtgaatg
     1981 gatctcgaga cttcgactgc attcgcgccg gcgcggcgat tgactgcaag gatcacggtt
     2041 gctgacggca cgggataacc gcgtacccag aagcgcatgt tgacgcgctc gtcctgcagc
     2101 gattgctcga tggtggcgac gtctcgcaag tagatgggtg cgttattggt gccgccgacg
     2161 attaagttgg catactgttc cggagtttcc agttgtcctt tggggttgag gatggccgtg
     2221 ctggaagcgc tatccagttg accagcgccg gtgtagctgg tgccactctt aatcgcagca
     2281 ttcagatcat caatcgaaat cccgcgcgcc cacatcgctg aggggttggc cttgatgcga
     2341 attgccgact tactgccaaa cacattcacc tgcgagacgc ctggaagaat actgagacgt
     2401 tgacctacct gggtactcgc atagtcatag agctggccgg tggttaccga gtcgctcgtc
     2461 aacgcgatgt acatgagcgc ctggtcgttg ggattgctct tggaataagt cggcggtgaa
     2521 ggaagatcgg cgggcagatt gccaatcgcc tgcgagattg ctgtctgcac atcggtcgcg
     2581 gcggcgtcga tgtttttctc gagcgcaaac tggagcgtga gtatggccac tccctgagtg
     2641 ctctgcgaag ttacaacctc cagtccattg atttgcatga actggcgttc aagcggcgta
     2701 gcgatattgt tggctatcgt atccggactc gccccgggat atcccaccac cacctgaata
     2761 accggatagt cgacggcggg aagatcatta accgggagct gaaaataact cagcactcca
     2821 aagaggatca ccgagagagt cagcacggcg gtcattacgg gccgccggat gaatggttcg
     2881 gagagattca tttcgaaccg ccactgttag ctgccgcggc gccagcctcc cgcggctgct
     2941 caatcagaac cttgccaccg ggcgttacgc caagttggcc attgataacg acctgttcac
     3001 cggctgccac ccctgactcc acaacgatca gatcgccctg gcgctggccc agcgacaccg
     3061 gacgttgttc ggcagttgag tcctgcttca ctacataaac aaaagatccc ttcgcggaca
     3121 tttgcggtgc agacgcagga actaaaacgg cgctgtgaat tgtgttcaag accaggcgaa
     3181 tgtttacaaa gcggccgggc cagaaacggt gatcactgtt gggaacggtc gcgcgcagag
     3241 ttacctggcc cgtcgtagtc tgtaccgcat tatcgagaaa ggtcagttgg ccggcgacca
     3301 tctcatccac catatcgggg agatggactt cgactttgag agcgcccgcc cgcatttgtt
     3361 gttgcactgc ggtgagactc gcttgcgaaa tggtgaagtc agcgtagatc ggatccagac
     3421 gttcgatcac cagcagcgca ttggtgttgc tggaacttcc gtcaccattg ttgttgcttg
     3481 agccgcccgg gttcaccacg ttgccgagat ctaccaaccg ttgtccggcg cggccatcaa
     3541 tgggcgaatg aatgtaacag taactgaggt tgaccttcgc attttcgacg gcggccgcgt
     3601 ctactttgat ttcttcatcg gcactgcgca ccgcagcgcg agcagcctca acgttcgctt
     3661 tgagggagtc gtagttcgct cgcaactctt cgtattgctc gcgcggaact acgccttgct
     3721 gcacgagggt gcgatacctt tcgacctgag caaggcccca gttcgcctga gcaatttcct
     3781 ttgccaggtt ggcttcagct tgtttcttga gcgctccgtc tttggagaga ttagcctgcg
     3841 cctgtctcag gctcgcttcg aacggcctgg tatcgatcgt gaagagcaga tcgccttttc
     3901 tgacgttagc accgtcggtg aaatggattt tggtgattcg tccagaaact tgaggttgaa
     3961 tcgaaaccac ttcacgcgcc actgtcttgc caagcgcgtc gatataggtc ggcacatcct
     4021 ggctgacggc tacgcttacc tggacgggtg acggtgggcg gtcaaagctc tgttgcgctt
     4081 tgttggcaca gccggacaac aacagggaga aagagatcgc caacagggaa actttaggga
     4141 gatagagcat ggttcaattg tatttcaagt cagccgtttt ggctgagtcg gggctggttt
     4201 ggggaaacgg ttaaaggatt cttacgcaaa tgggggctgg agggttctgc ttctgattac
     4261 tttttgggga tgggttgctt ttaaatcaag catctgaccg cagtaggctc gatgtctcat
     4321 tctcaccggc ctttaggccg gtgctggtgc gcccatctca ttctcaccgg gcttcagccc
     4381 ggtcaaacac atcaggacct ctcattctca ccgggcttca gcccggtcaa tcagatcagg
     4441 acctctcatt ctcaccgggc ttcagcccgg t
//
LOCUS       DASUEX010000285         4118 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_58044, whole genome shotgun sequence.
ACCESSION   DASUEX010000285 DASUEX010000000
VERSION     DASUEX010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4118
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58044"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..677
                     /locus_tag="VFV61_04775"
     CDS             <1..677
                     /locus_tag="VFV61_04775"
                     /inference="COORDINATES: protein motif:HMM:NF012974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DnaB-like helicase N-terminal domain-containing
                     protein"
                     /protein_id="HEX4899823.1"
                     /translation="SNPAHKEAAIEEVLPFIRAVRSRIQKREYFDIAMDSLRVSDLSL
                     RREFWQSLRGGETARSVVKRGASGHAEATPAEQRLLGVLLADENLRRQILPLLHTEDY
                     EDLPTAAIFRALSELEQEHREVNFESLSAKTEGDALATRLLPMLLMSESLHASNEHYA
                     AAECVQTFRLMKINHRIEEIMAERAAAEREENNEKVNSLTLEQVELARRRADLMPRLE
                     AMQTSN"
     gene            808..2538
                     /gene="rpoD"
                     /locus_tag="VFV61_04780"
     CDS             808..2538
                     /gene="rpoD"
                     /locus_tag="VFV61_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012607388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEX4899824.1"
                     /translation="MGIEDKDRDDVMDRLLELGGDKKYLTFDDLNRELPDNVVSPDDI
                     EDVLQKLEGSNIPVADSDERLLEQAAVTVPDDDDEAIDEDVELDLSAGALEKTNDPVR
                     LYLREMGVVPLLTREGEVAIAKRIERGQLKTQKSIARSPLAVKELLRIGEQLEAGQAN
                     IREIVLFSEQAELAEDADKAEEYRNWTIEGIQNIRKLYKNALKEWDKLRAEQKATRGK
                     KSKKLMRLRRKVARLRLEIAQEIGRLNMTERARQRLINAIRAVQKEVREADREIASFT
                     EKLGKKRIKPDDQKEYKRRISAAKKRLVLIEAEHSVSPVEIKRSLQSITIGEQQTGQA
                     KRELVEANLRLVVSIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEWRRGYKFSTYATW
                     WIRQAITRAIADQARTIRIPVHMIETINKLIRTSRALVQELGREPTSEEIAKKMDIPV
                     SKVRKVLKIAQEPISLETPIGEEEDSHLGDFIEDKSILNPADAVVASNLREITDEVLA
                     TLTPREEKVIKMRFGLGTTGSEHTLEEVGQHFAVTRERIRQIEAKALRKLRHPSRSRK
                     LKAFLDGAQR"
     gene            complement(2661..3044)
                     /locus_tag="VFV61_04785"
     CDS             complement(2661..3044)
                     /locus_tag="VFV61_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899825.1"
                     /translation="MVSIYKPDERGSARLNFILFMALVIVGAYVGYLSLPVAYNAYVW
                     KDLMQHKVDVAATQGYPATWVNDQLKKSAGEYGIPADAEITATPLDQRIQVRVQYTKP
                     IEFPGYTYQYEFDHTAKSTEFLTIK"
     gene            complement(3067..>4118)
                     /gene="aroB"
                     /locus_tag="VFV61_04790"
     CDS             complement(3067..>4118)
                     /gene="aroB"
                     /locus_tag="VFV61_04790"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEX4899826.1"
                     /translation="GIARRALGLQTRRTVVVSNRRVFDLYGSRLMQSLRTNGFQVTSW
                     LMGDGERFKTAPTAEKAVRAFSTAGLERTDAVIALGGGVVGDLAGFAAAIYLRGIPLI
                     HVPTTMTAQLDSAIGGKTGVNLAHAKNAIGSFHQPRAVIIDVETLKTLPRRELVAGWC
                     EAVKNGAIGSRKLFETTVMFLSQSQNSVASPRLISLIKSHCEFKAHIVSGDERESAIR
                     TDQRSRRVLNFGHTIGHALEAVTNYRRFRHGEAVGHGMRVAAEMSKSMGLLKPSELRL
                     LCDGIDLCGPLPPADDIDANEILRLTRADKKSSRGRVQWVLLEEIGHPRIIADQEIPR
                     RLIREAVRRGLASTS"
BASE COUNT         1006 a         1091 c         1153 g          868 t
ORIGIN      
        1 tcagcaatcc ggcccacaag gaggccgcga ttgaggaggt gcttccgttt attcgcgctg
       61 tccgcagccg gattcagaaa cgggagtact ttgatatagc aatggactcg ttgcgcgtca
      121 gcgacctatc gctgcggcga gagttctggc agtcgttacg cggcggcgaa actgctcgat
      181 ctgtggttaa gcgcggtgcc agcggccacg cggaagcgac tccggctgag cagcgtttgc
      241 tgggtgtgct gcttgctgac gagaatctgc gccggcagat tctgccgctg ctgcatacgg
      301 aagattacga agatttgccg acggcagcga ttttccgggc gctaagtgaa cttgagcagg
      361 aacaccggga agtcaatttt gagagcctca gcgccaagac ggaaggtgac gccctggcga
      421 caaggctcct gccaatgttg ctgatgagtg agtccttgca cgcctcaaac gagcattatg
      481 cggctgcgga atgcgtgcag acgtttcgct taatgaaaat caatcatcgc attgaagaga
      541 tcatggctga acgggcggca gccgaacgag aggaaaataa cgaaaaggtt aattcattaa
      601 cacttgaaca ggttgagctt gctcgtcgtc gggcagacct aatgccaaga ctggaagcaa
      661 tgcagacgag caactgaaat tcttgtatct aaatcgcaat ttgggactcg cgcattcgtt
      721 ctctaattta gctggaaaca ttggcagcga atgggaaccc ccggcagccg gagcgcgaga
      781 cccgcaaacg tttgatggag agataagttg ggtattgaag ataaagatcg tgatgatgtg
      841 atggaccgtc tactagagtt gggtggcgat aagaagtact taacgttcga cgatctcaat
      901 cgtgagttgc cggacaatgt ggtttcaccc gacgacattg aagacgtcct gcaaaaactc
      961 gaaggctcaa atattccggt agcggactcc gacgagcgcc tgctcgaaca agcggccgtt
     1021 actgtcccgg acgacgatga cgaggcaatc gacgaagatg tcgagctgga tctgtcggca
     1081 ggcgcccttg agaaaaccaa tgacccggtg cggttgtact tgcgcgaaat gggggtcgtg
     1141 ccgctgctta cgcgtgaagg cgaggtagcg attgccaaac gcatcgaacg tggtcaactg
     1201 aagacgcaaa aatctattgc ccgttcgccc ctggcagtaa aagaactgct gcgcatcggc
     1261 gagcagctgg aagcgggtca ggccaatatt cgcgagatcg tgcttttttc cgaacaagcg
     1321 gagctcgcgg aagatgccga caaagcggag gaatatcgca actggacaat tgaaggcatt
     1381 caaaatatcc gcaagctcta taagaatgcc ctcaaggaat gggacaagct acgcgccgaa
     1441 caaaaagcca cgcgcggcaa gaagagcaag aagctaatgc gcttgcgacg caaggtcgca
     1501 cgcctgcgtc tggagatcgc gcaggagatc ggccggctca acatgaccga gcgcgcgcgc
     1561 cagcgtctaa tcaatgcaat tcgcgccgtg caaaaggaag tgcgggaggc agaccgtgag
     1621 atcgccagct ttacggaaaa gctcggcaag aagcgcatca agcctgacga tcagaaggaa
     1681 tacaaaagac gcatctcggc cgccaagaag cgcctggtct tgatcgaagc cgaacacagt
     1741 gtcagcccgg tggagataaa acggtcgttg cagtcaatca ccattggcga acagcaaacc
     1801 ggccaagcca aacgagaact cgtggaagcc aatcttcggc ttgtggtgtc gattgccaag
     1861 aaatatacga atcgtggctt gcagtttctg gatctgattc aggaaggcaa catcggtttg
     1921 atgaaggcgg tggacaagtt tgaatggcgt cgcggttata agttttccac ctatgcgacg
     1981 tggtggatcc ggcaggcaat cactcgcgcg attgccgatc aggcgcgtac gatccgcatt
     2041 cccgttcaca tgatcgaaac gatcaataaa ctcattcgca cgtcacgggc cctggtgcag
     2101 gaacttggcc gcgagccgac gagtgaagag attgccaaga agatggacat cccggtttcc
     2161 aaggtgcgca aggtcctgaa gattgcccag gagcccatct cgctggaaac accaattggg
     2221 gaagaagaag actcgcatct cggggacttc attgaagaca agtcgattct taacccggcg
     2281 gacgcagtcg ttgcttccaa ccttcgcgaa atcaccgacg aggtcttggc aacgctgact
     2341 ccccgagaag agaaagtcat caaaatgcgg tttggtctcg gcaccaccgg ttccgaacat
     2401 acgctcgaag aggtaggcca acactttgcg gtaactcggg aacgcattcg gcaaattgaa
     2461 gccaaggccc tgcgcaaact gcgccatcct tctcggtcac gaaaactaaa agcgtttctc
     2521 gatggcgcgc agagataagt cagctgcggg accggagcag agctaaagaa gaacacgatt
     2581 tagataagta aagggcgctg gatcaaatcc ggcgcccttt tctgcggtct caacctgcca
     2641 acttttgtcg ttagacttgc tcacttaatg gttaggaatt cggtgctctt tgccgtgtgg
     2701 tcgaactcgt actggtacgt atacccggga aactcaatcg gcttcgtgta ttgaacgcgc
     2761 acctggattc tttgatccag aggcgtggcg gtaatctcgg catccgcagg aatgccgtac
     2821 tcacctgcac tcttcttgag ttgatcgttc acccaggtgg cgggatatcc ctgcgtcgcg
     2881 gctacatcaa ctttgtgctg cattagatct ttccaaacgt aagcgttgta ggcaacgggc
     2941 agtgaaaggt aaccgacata cgcgcccaca atgaccagcg ccatgaacag aataaaattt
     3001 agtcgagcgc tgccgcgttc gtctggcttg taaatgctca ccatagctat cgtcctcaaa
     3061 tatcactcag gaagtggacg ccaggccccg gcggactgcc tcgcgaatca ggcgtcttgg
     3121 aatttcctgg tccgcgatta ttcgcgggtg gccaatctct tcaagtaaca cccattgcac
     3181 gcgtcctctt gagctcttct tatccgcccg cgtcagccgc agtatctcat tcgcatctat
     3241 gtcgtctgcc gggggcaacg ggccacacag atcgattccg tcgcacaaca atcttaactc
     3301 cgatggcttg agcagcccca tactttttga catctcagcc gcgacgcgca ttccatgacc
     3361 aacggcttca ccatgacgga agcgtctgta gtttgtgacg gcctcgagcg catgaccaat
     3421 ggtgtggccg aaattgagca cacgtcgcga tctctggtcg gttctaatcg cgctttctcg
     3481 ttcatccccg ctaactatgt gcgctttgaa ctcacaatga gatttgatca gactgataag
     3541 ccgcggcgac gcaacactat tttgggattg ggataagaac atcacggtcg tttcaaaaag
     3601 tttccgactg ccgatcgcgc catttttaac ggcttcgcac cagccagcca ctaactcgcg
     3661 ccggggcagc gttttgagcg tctccacatc aatgattacg gcccttggct gatgaaatga
     3721 gccgatcgca ttttttgcgt gcgccaggtt aactccagtc ttacctccga tcgctgaatc
     3781 taattgcgca gtcattgtcg ttgggacgtg aattagagga atgccgcgaa ggtagattgc
     3841 ggcggcgaaa ccggctagat caccaacgac gcccccaccg agggcaatca ccgcgtcggt
     3901 ccgctcaaga ccagcggtgc taaaggcccg cactgctttt tccgccgtcg gcgccgtctt
     3961 gaatcgctcg ccatctccca ttagccatga ggtgacctgg aagccgttgg tccggaggct
     4021 ctgcattaac ctgctgccgt agagatcaaa aactctgcgg ttggagacta cgactgtgcg
     4081 acgggtttgg aggcctagcg cccggcgggc aatgccac
//
LOCUS       DASUEX010000286         3325 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1547645, whole genome shotgun sequence.
ACCESSION   DASUEX010000286 DASUEX010000000
VERSION     DASUEX010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3325
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1547645"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="VFV61_04795"
     CDS             complement(<1..510)
                     /locus_tag="VFV61_04795"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX4899827.1"
                     /translation="MSTPALHPQGQPLNPERRGVKKGLYLIPSVFTAANIGMGFYAVM
                     AAARGFQFVGTGVEQDLIRASTYLDNAAMAIGWAVLFDMLDGRIARMTKTTTEIGVQL
                     DSIADVVTFGLAPAVLAYVWGYGAALREGSDLHRLAWFLSFMYLMCGAFRLARFNVQS
                     SRPRILAEGT"
     gene            complement(510..1145)
                     /locus_tag="VFV61_04800"
     CDS             complement(510..1145)
                     /locus_tag="VFV61_04800"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:NF003678.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HEX4899828.1"
                     /translation="MSIARDGLPYILIPAAASLVLLALGYWYGTGYWYAAVPFVLVTA
                     FMAYFFRDPKREPPQDTAAVVAPADGKVTRVGAIADTLDAPTLVSIFLSPLDVHINRA
                     PIAGTVTNVSYTRGKFLMATDKNASLVNEQNALTIQGDKITVVCKQIAGILARRIICW
                     KHAGEHVNLGERFGLIKFSSRTDVLLPVGVEVLVTEGMRVRGGVTIIGRIK"
     gene            complement(1263..1505)
                     /locus_tag="VFV61_04805"
     CDS             complement(1263..1505)
                     /locus_tag="VFV61_04805"
                     /inference="COORDINATES: protein motif:HMM:NF016231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HEX4899829.1"
                     /translation="MDMTTTDTIKEELMNHDPEFREMAREHTRYEERLSELSALTYPS
                     DEEQLEEITLKKKKLALKDQMYSLMLQYQKNQALSH"
     gene            complement(1628..2086)
                     /gene="rimI"
                     /locus_tag="VFV61_04810"
     CDS             complement(1628..2086)
                     /gene="rimI"
                     /locus_tag="VFV61_04810"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEX4899830.1"
                     /translation="MTEHDLLEVVEIEEQSRLSRWGWAAYYAELQGINRPLMLVARIN
                     GRAEGLHRNRVAGYIVARFSAGELHINNVAVRSEYRGRGIGKALLARILAQGKKLNAA
                     AAFLELRAGNSIAQSLYEKAGFVPVGKRRNYYSDPVEDAVTMSAQLKGSA"
     gene            complement(2128..2868)
                     /gene="tsaB"
                     /locus_tag="VFV61_04815"
     CDS             complement(2128..2868)
                     /gene="tsaB"
                     /locus_tag="VFV61_04815"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEX4899831.1"
                     /translation="MKPLILSIETATLGGGVCLSRGDSIIATISGDPKLSHSNTLLKD
                     INECLQEVSTTVQDVELFAATSGPGSFTGLRIGLATIKALAATLNRPCLGVPTLQVIA
                     HAGGVSSATVSLLPAGRGEVFVQLLSVAHDVTELDTAAHLPPARAIERYKSLPDVTWV
                     GPGAAIHRNAIESTARKQGFAFQAVDVNDSAMNAEGWCLVSRETNLASHVAAIAWQRF
                     QRQELEQAESLSAIYVRPSDAELKCNPA"
     gene            complement(2885..>3325)
                     /locus_tag="VFV61_04820"
     CDS             complement(2885..>3325)
                     /locus_tag="VFV61_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899832.1"
                     /translation="GFGWRFAHSSQGARMALDANQPFAGKQSLLVTFLGYSEPNTALL
                     SQLLLVEPHKHYQVKFEARAENLLTGGLPVITVADASGNKNILGQSDAIRSERPAWQS
                     LSFDFRTNNETAVELRLQRKPCNTVPCPIFGSLWLDNFTIAEVK"
BASE COUNT          752 a          945 c          886 g          742 t
ORIGIN      
        1 agtgccttcc gcaaggatgc gcggccggga cgactgcaca ttaaaccggg ccaggcgaaa
       61 cgcgccgcac atcaggtaca tgaacgagag aaaccacgcc aggcgatgca ggtcacttcc
      121 ctctcttagc gcggcgccat aaccccagac gtaagccaga acagcgggcg cgagaccaaa
      181 tgtaacaacg tcagcaatgg aatcgagctg cacgccaatc tcggttgttg ttttcgtcat
      241 gcgggcgatg cggccgtcaa gcatatcgaa aagcaccgcc caaccgatgg ccattgcggc
      301 gttatccagg taggtgctcg cgcggatgag atcctgctcg acaccggtac cgacgaactg
      361 aaatccccgc gccgctgcca ttactgcata gaagcccatt ccgatgttag cggcagtaaa
      421 aaccgaggga atgagataga gacccttttt aacgccgcgg cgttcgggat ttaggggttg
      481 accctgcgga tgcagcgctg gggtgctcat cactttatcc tcccgataat agtgactcca
      541 cctcgaactc tcattccttc agtcactaac acttcaacgc caacgggcag cagcacatca
      601 gtccgagagc tgaacttgat taggccaaag cgctcgccga ggttgacgtg ctcgcccgcg
      661 tgtttccaac agatgatgcg acgggcaagg attccggcga tctgtttgca cacaacagta
      721 atcttgtcgc cttgaatcgt cagcgcgttt tgctcattta ccaaactcgc gtttttatcg
      781 gtcgccatca gaaattttcc gcgcgtgtaa gaaacatttg tcaccgttcc cgctattggc
      841 gcgcggttga tgtgaacatc caacggagag agaaaaatac tcacgagcgt gggcgcatca
      901 agcgtgtcag cgatggcacc tacgcgcgtg actttgccat cggccggcgc aaccactgca
      961 gcggtgtctt gcggtggttc gcgttttgga tcccggaaga agtacgccat gaacgccgtg
     1021 accagaacga acggaaccgc cgcataccag taacccgtcc cataccaata acccagcgcc
     1081 agcaacacta acgacgccgc tgcaggtatt aggatgtagg gaagtccgtc gcgcgcaata
     1141 ctcaaaatga tgtcctggtg tcgtgggctg aaactctcga ggttcggcta caaaaagggg
     1201 acgcgtaacc ggaggtccac gtccccaatg atctcaaccc acagaagctt gttcggcccg
     1261 aattaatggc tcagtgcttg attcttttga tactgcaaca ttaatgaata catttgatct
     1321 ttgagtgcaa gtttcttctt ctttaatgta atttcttcca gttgttcttc gtcgctcgga
     1381 tatgtcaggg ctgaaagttc actgagtcgc tcctcatagc gagtgtgttc gcgagccatt
     1441 tcgcgaaact caggatcgtg gttcatcaac tcttctttga tcgtatccgt cgttgtcatg
     1501 tccatgtgcg tggatatctc ctttcaggat gaagaactgg taagaatttg ggttggcgga
     1561 ggcggtcact acgaacctcc taagatgttt ggccacgaaa aaatggtagc ccaattcata
     1621 cggcgaatca agcactccct ttgagttgag cagacatcgt aacggcatct tcgaccgggt
     1681 cggaatagta attacggcgc tttccgaccg gaacaaagcc cgccttctcg taaagcgact
     1741 gggcaatgga gtttcctgcg cgcagctcca gaaaagccgc agcggcattg agtttcttcc
     1801 cctgcgccag gattcttgcg agcagcgctt tgccaatgcc acggccgcga tactcgctgc
     1861 gcaccgccac gttgtttatg tgcagttcgc ccgcggaaaa tcgggccacg atatagccgg
     1921 caacgcggtt tctgtgaagt ccctcagctc taccattgat tcgtgctacc agcatcaggg
     1981 ggcgattaat accctggagc tctgcgtagt aggccgccca gccccaacgg ctgagacggg
     2041 actgctcttc aatttcgacg acttcgagca gatcatgctc cgtcatggtt gagatcgaaa
     2101 tcggctgcag cgaactttcg ctgtttgcta ggctgggttg catttcaatt cggcatccga
     2161 aggacgtaca tagattgcag acaaactctc ggcctgctcc agctcctggc gctgaaaacg
     2221 ctgccaggcg atggcggcga catggctcgc caagtttgtt tcgcgcgaca ccaggcacca
     2281 accctcggca ttcatcgcac tatcattcac atccacagct tggaatgcaa agccctgttt
     2341 ccgcgcggtc gactcgattg cattgcgatg tatggctgcg cccggaccga cccaagttac
     2401 atccggcaac gatttatacc tttcgatcgc gcgtgccggc ggcaggtgcg cggccgtgtc
     2461 cagctctgtg acatcgtgcg cgacggaaag taactgcaca aaaacctccc cgcgtccggc
     2521 gggcaacagc gacacggtgg cgctggaaac gccacctgcg tgggcgatga cttgcagcgt
     2581 ggggacaccg aggcagggac ggttcaaggt ggccgccagg gcctttatcg ttgccaaccc
     2641 gattctaaga ccggtgaagc ttcccgggcc acttgtggct gcgaacaact ccacatcttg
     2701 aacagtggta ctgacctctt gcagacactc gttaatgtct ttgagcaaag tgtttgagtg
     2761 ggagagttta ggatccccgg aaatcgtcgc gatgatgcta tcgcctcgcg aaaggcaaac
     2821 gccccctccc agagtcgcag tttcaatgct caaaatcagc ggcttcattg gcaaatcgat
     2881 tctctcattt cacttccgcg atcgtgaagt tgtccagcca gagcgaaccg aaaatcggac
     2941 aaggaacagt gttgcagggc ttgcgctgta atctcaattc cacggctgtc tcattgttag
     3001 ttcgaaaatc aaagctgagt gactgccagg ccggtctctc agatctgatc gcgtcggatt
     3061 gtcccagaat gttcttgttg ccgctggcat cggctacggt aatcaccggc agcccacccg
     3121 ttaacaggtt ttccgcgcgc gcctcgaatt tcacctggta atgtttgtgc ggttccacca
     3181 atagcaactg tgacaagagc gccgtgttcg gttcggaata accgaggaac gtcacgagca
     3241 ggctttgctt ccccgcgaag ggttgatttg cgtccaacgc catccgcgct ccctgggaag
     3301 agtgagcaaa acgccaccca aagcc
//
LOCUS       DASUEX010000287         2321 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_628561, whole genome shotgun sequence.
ACCESSION   DASUEX010000287 DASUEX010000000
VERSION     DASUEX010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_628561"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1357
                     /locus_tag="VFV61_04825"
     CDS             <1..1357
                     /locus_tag="VFV61_04825"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX4899833.1"
                     /translation="FLRVAFWNIERGLQYEAVAGAFGGSRDYTAMLHRTKLAHSRATR
                     NAAAKEAALLSQADVIVLNEVDWGMKRTDYRNVIADLAAALRMNYAYGVEFVEVDPIA
                     LGTEKFEGVEEGAAELIKDIKVDTSKYKGLHGTAILSRYRLENVRLQPFQFQAHDWYE
                     DEKNGVAPVEKGKRKVAEQVFLEKIYRQVRRGGRMLLRADLVDPQFPQGKVTIIATHL
                     EDRTTPKNRQKQLEELLAQIKDIDSPVILTGDMNTTGEDSTPTSLTREVTKRLGSKSF
                     WIHRGIGYALGVGILKDVLIGGVKFVRNQSDPTVKHVPIVAPNPAAGFFDMLKDFRFK
                     DGGAFDFRGEPQRSIGASSETLGNSNERGPKGFVTTFEVERKVASLGKFKLDWFFVKP
                     PGLTDPDKKDQPHLFAPHFGRTLKPLNYSIEERISDHSPIIVDLPFQEPRIGETPVRK
                     Q"
     gene            complement(1363..1737)
                     /locus_tag="VFV61_04830"
     CDS             complement(1363..1737)
                     /locus_tag="VFV61_04830"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEX4899834.1"
                     /translation="MKIGDQISWQRTFTEADIRAFTELSGDAGEHHLLPDPQGRLMAH
                     GLLTATLPTKIGGDMNFIAREMTFQFHRPVFSGDTIKCEVTVSSLEQVEQLTNITTTF
                     VCRNQHGKEVMTGIARGVIRTV"
     gene            complement(1740..>2321)
                     /locus_tag="VFV61_04835"
     CDS             complement(1740..>2321)
                     /locus_tag="VFV61_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899835.1"
                     /translation="TRAGLVIAQMDWQIFSVKRLESWQVYKDKEKVLAATLVYLPASK
                     EVAVTLPFMNRPTENVAIPFLPFHVYNFDFASLNFAFPHLVNPKKRFRVGVSDPTFSD
                     QGPAFRYRGEVDVAYVADEMRNGFLCRKYRIDGPGLENKGGFIWVNKRSGYFQDAEIQ
                     LADNPNWQTFKFKLQKVDRMSAAQWAAFMKAQF"
BASE COUNT          562 a          615 c          623 g          521 t
ORIGIN      
        1 gttccttcga gtcgcctttt ggaacatcga acgcggcttg cagtacgaag cagttgccgg
       61 ggccttcggc ggttctcgcg actacaccgc tatgctgcat cgcaccaagc tggcgcattc
      121 ccgcgctacg cgcaacgcgg ccgcgaaaga agcagcgctg ctgagtcagg ctgatgtgat
      181 tgtcctcaat gaagtcgact ggggcatgaa gcgtaccgat tatcgcaacg tcatcgctga
      241 cctggccgcc gccctgcgaa tgaattacgc ctatggcgtc gagttcgttg aagtagatcc
      301 aattgcgctc ggcacggaga agtttgaagg tgtggaggag ggagccgcgg agttaatcaa
      361 agacatcaag gtcgatacat caaagtataa aggtcttcac gggacagcaa tcctcagtcg
      421 ctatcgtctc gaaaatgttc gtttacagcc gttccagttt caggctcatg actggtacga
      481 ggacgaaaag aacggcgtgg cacctgtgga aaaaggcaag cgcaaagttg ctgaacaggt
      541 tttcctggaa aaaatctacc gccaggtaag acgaggcggg cgcatgttgt tgcgagctga
      601 cctggtcgat ccgcaattcc cgcaaggcaa ggtaacgatc attgcgactc acctcgaaga
      661 tcgcaccacg cccaagaatc gtcagaagca actcgaggaa ctacttgcgc aaattaaaga
      721 catcgactcg ccggtaatct tgaccggcga tatgaatacg accggggagg actcgacacc
      781 aacctcactg acaagagagg tgaccaaacg tcttggcagc aaaagcttct ggatccatcg
      841 cggcatcggg tacgcactgg gcgtcggtat cctcaaggat gtattgattg gcggagtgaa
      901 gttcgtacgc aatcaatcag accccaccgt caagcatgta ccgatcgtcg ctcctaatcc
      961 ggcggctggt ttcttcgaca tgttgaaaga ctttcgcttt aaggacggcg gcgcctttga
     1021 ttttcgcggc gagcctcagc gttcaatcgg tgcgagttcg gaaacgttgg gcaattcgaa
     1081 cgagcggggt cccaaaggat ttgtcaccac tttcgaagtt gagcgcaagg tagcttcgct
     1141 gggtaaattc aagctggatt ggttctttgt aaaaccacca ggtctgactg accctgacaa
     1201 gaaagatcag cctcatctgt tcgcgcctca ctttggccgc actcttaagc cacttaatta
     1261 cagcatcgag gagcgcattt cggaccacag ccccatcatt gtggatttgc catttcagga
     1321 accgcgtatc ggggagacgc cggtccgaaa acaatgatcg ctttaaacgg tgcggattac
     1381 cccgcgcgct attccggtca ttacttcctt gccgtgttgg ttgcgacaga cgaatgtcgt
     1441 cgtgatgttc gtcagttgct caacctgctc cagcgaagac acagtcacct cgcacttgat
     1501 cgtgtcgccg gaaaaaacag ggcgatggaa ctggaaagtc atttcgcggg cgatgaaatt
     1561 catatcgccg ccaatcttgg ttggcagcgt cgctgtcagc agcccatgag ccatcaaccg
     1621 accttgcgga tccggtaata agtgatgctc gcctgcgtcg cccgagagct ccgtaaaagc
     1681 gcgaatgtcc gcttcggtaa aggttcgttg ccaggaaatc tggtcgccaa tcttcatgat
     1741 cagaactgcg ctttcataaa cgctgcccac tgagccgcgc tcatgcgatc aactttttgc
     1801 agtttgaatt tgaacgtttg ccagttggga ttgtcggcaa gttggatttc agcgtcttga
     1861 aaatagccgc tgcgcttgtt gacccaaata aagcccccct tgttctctaa tcctggccca
     1921 tcgatgcggt atttacgaca aagaaaccca ttgcgcattt cgtcggcgac ataggcgacg
     1981 tcgacctcgc cgcgatagcg aaacgccgga ccctgatcgc tgaatgttgg atcactaact
     2041 ccgaccctaa accgcttttt gggattgacg aggtgcggaa acgcaaaatt caagctggca
     2101 aaatcgaagt tgtagacgtg aaaaggaagg aaggggatgg cgacgttctc agtaggacga
     2161 ttcatgaacg gaagggtgac agcaacctct ttgctcgccg gaagataaac gagtgtggct
     2221 gcgagcacct tctctttgtc cttatagacc tgccacgact cgagacgctt cactgaaaaa
     2281 atttgccagt ccatctgcgc gatcactaat cccgcccgcg t
//
LOCUS       DASUEX010000288         3104 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1181060, whole genome shotgun sequence.
ACCESSION   DASUEX010000288 DASUEX010000000
VERSION     DASUEX010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3104
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1181060"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..876)
                     /locus_tag="VFV61_04840"
     CDS             complement(58..876)
                     /locus_tag="VFV61_04840"
                     /inference="COORDINATES: protein motif:HMM:NF016952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HEX4899836.1"
                     /translation="MRKNCRLGAELALVLLFAAFNLGCQSGANVNSAGNNTPASSNAK
                     VGYEIVNAFPHDANAYTQGLIFTNGVLLESTGREGQSSLRRVELQTGKILNQVNVPRP
                     YFAEGLALLNGKLFQLTWQHGVGFIYDATTFEKLGEFKYSGEGWGLTTDGNSLILTDG
                     SHRIRFLDPETFAVRKTISVLDHGRVIDSLNELEYIKGEIYANIWHDQRIARIDPNTG
                     RVNSWIDLTGLREASGATEEEGVLNGIAYDAAGDRLFVTGKLWAKVFEIRLKQN"
     gene            1173..2843
                     /locus_tag="VFV61_04845"
     CDS             1173..2843
                     /locus_tag="VFV61_04845"
                     /inference="COORDINATES: protein motif:HMM:NF014214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M36 family metallopeptidase"
                     /protein_id="HEX4899837.1"
                     /translation="MRQQFKSVLVIIIGLGLIVSSSFATSTPLQTAPSKMSPSQTVPQ
                     NRQEEGQPAPEVFPYWQALAAKSDEPVSVSWNKRSGTPQTIFARLPNPAKGVGEVSAR
                     AFLNQNAELFKLGQDAELNLIRNVESPIGQHVVFEQRYAGILVYGAQVAVHFNRSGDI
                     IVVNNSYQPDISLPGTTPRVSKASAVGQSLALVKETSSSRSSGELVVIDFKGAFRLAW
                     RIVVATDGPTWEVFVDALNGKTLGSARDINRYVNGSGQVFVVNAIVATRDNSLRDMAD
                     SAAAVPLSAYSTVTLQGLVGNGFLDGDFASSSRTKKRAFNAVNNFAFDRSDDGFSETM
                     GYYYLDYAERYIQSLGFTNINNRQQVFGVNRLKIDNSFYSPQSKEISLGLGGVDDAED
                     AEVILHEYGHSIQDNQVPGFGSTLEAGSMGEGFGDYWGASVGAQFSGGFQDLCLAEWD
                     ATSYSSTNPPCLRRLDSTKHYPEDVAGEVHDDGEIWAAALWQIRGSLGATDSDRLVLA
                     AHFLLAPTASFNQGANALVTAAISLGYSNHKVNIVRTTLRNRGFTVTV"
BASE COUNT          747 a          835 c          805 g          717 t
ORIGIN      
        1 catggcgtag gaggaacctc tgaaataagt ctcgaaactc ataatgctcg ctggttatta
       61 gttttgcttt aggcgaatct caaacacttt tgcccaaagt ttacccgtaa caaaaagcct
      121 gtcaccggcc gcatcgtaag caatcccatt gagcacgccc tcttcctcag tcgccccact
      181 cgcctcacgc agtcccgtaa gatcaatcca gctattaacg cgacctgtgt tgggatcgat
      241 gcgcgcaatc cgctgatcgt gccagatgtt cgcgtagatt tcgcctttga tgtattccaa
      301 ctcattaaga ctgtctatta cccggccgtg atccaacacg ctgatggttt ttctaaccgc
      361 gaaggtttcg ggatcgagga aacgaatccg atggctccca tcggtgagaa tcaaggaatt
      421 gccgtcagtc gttagacccc agccctcgcc tgagtactta aactcaccca gcttctcaaa
      481 cgtcgttgcg tcgtaaatga atccgactcc atgttgccag gtcaattgga aaagctttcc
      541 atttagtaga gccaaaccct cggcaaagta cggccgggga acgtttacct gattgagaat
      601 tttccccgtc tgcaattcaa cgcggcgaag cgatgactgg ccttcgcgac cagtgctctc
      661 caataagacg ccgttcgtga agatcaatcc ctgtgtataa gcgtttgcgt catgcggaaa
      721 ggcgtttacg atttcgtatc ccaccttcgc attcgaactt gccggggtat tgttgcctgc
      781 cgaattcaca ttggcgccac tttgacatcc gagattgaac gccgcgaaaa gcagcaccag
      841 agctagttcg gcccccagcc ggcaattttt ccgcaccagg gaagtcagtc ttccggaaac
      901 cgtcatgcgg gtattgtggg ggacaggtcg ggaaaaaaca aaaaggccct gctggattgc
      961 cggaatgctg gcgattgccg cttgatttgg ttcgccaaag agcccgccag aaggtagaat
     1021 cccgccgaat cctggctaga tgtatttgct agatcgacct gacaggtcta gacccgcgat
     1081 agcagatcag ttacgtgcgg aagctaccgc cctagtttcg aaaactactg atcctcgctt
     1141 gaggaatcga aaaaacaagg gagctcaaaa agatgagaca gcaattcaaa tccgtgttag
     1201 tgataatcat cggcctcggg ttaatcgtca gctcaagctt cgcgacgtca accccattac
     1261 aaaccgctcc aagcaaaatg agtccttcgc agactgtccc tcaaaaccga caggaagaag
     1321 gacagcctgc cccagaagtg tttccttact ggcaagcctt ggcagcgaag agtgacgagc
     1381 cagtttcagt cagctggaac aaacgcagcg gaacgcctca gactatcttc gcaagacttc
     1441 ccaacccggc gaagggtgtg ggcgaggtca gtgctcgtgc attcctgaac cagaatgccg
     1501 agttgttcaa actgggccag gacgctgaac taaatttgat tcgtaacgtc gaaagtccca
     1561 ttggacaaca cgtggtcttc gaacagcgct atgcagggat tctggtttac ggagctcaag
     1621 tggcggttca cttcaatcgc tcaggcgata tcatcgtggt aaacaattcc taccagcctg
     1681 acatttcttt gccaggaaca actccgcgag taagtaaggc aagtgctgtc ggtcagtccc
     1741 tggctcttgt caaagagact tcttcatcac gctcatcagg cgagctcgtg gttatcgatt
     1801 tcaaaggggc gttcaggctg gcttggcgaa tcgttgtggc gaccgacggg cccacctggg
     1861 aagtgtttgt cgacgcactg aatggcaaga cgttgggctc ggcccgcgac atcaaccgct
     1921 atgtaaacgg aagtggacag gtgttcgtcg tgaacgcgat cgttgccacg cgcgacaatt
     1981 ctctgcgcga catggccgac tcggccgcgg cagttccgct tagtgcttac tcaaccgtta
     2041 cgcttcaagg tctggttggg aatggctttc tcgatggcga ctttgcttca agcagtcgca
     2101 cgaagaaacg agcctttaac gcggtgaata attttgcttt tgatcgcagc gacgatggct
     2161 ttagcgagac catgggttac tactacctcg attacgcaga gcgctacatt caatcgctag
     2221 gcttcaccaa cattaacaat cgccagcaag tcttcggtgt caaccgtctt aagattgaca
     2281 actcattcta ctcaccgcag tcgaaagaga tttccctggg gttaggcgga gtcgacgacg
     2341 ctgaggacgc cgaagtgatc ctgcacgaat acggtcactc aattcaggac aatcaagttc
     2401 ccggcttcgg cagcactctc gaagccggtt cgatgggcga gggattcggt gattactggg
     2461 gcgcgtcagt cggtgcgcaa tttagtgggg gctttcagga tctctgcctt gcggaatggg
     2521 atgccacgtc ttacagcagc accaacccgc cttgcctgcg ccgactggac agcaccaagc
     2581 attatccgga agacgtcgca ggtgaagtcc acgacgacgg agagatttgg gcggcggctt
     2641 tgtggcaaat tcgcggatca cttggagcaa ccgattctga tcggctagtg ctggctgcgc
     2701 acttcctttt ggctccgacg gcctcgttca accagggagc taacgcactg gttactgcag
     2761 ccatcagtct cggctactcg aaccacaagg tgaatatagt gagaacgact ttgaggaatc
     2821 gaggattcac ggtcactgtg taactggtga gccggctcgt ttctaatctc acgcatgaaa
     2881 gcccgcgaga gcattaacac ttctcgcggg cttcttatat aatcgcgaag ccttctaaac
     2941 tccccacaga attaccttcg cacacaaccg gctgagccga agtcgccact gtgataatcg
     3001 gactggacct tcgtttgcca aagaaaaaag taatgagaag actctcgttt gtggggacgt
     3061 gcttcgtggt tcggactagc aactgcagat tagactcggc cgtc
//
LOCUS       DASUEX010000289         3738 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1639709, whole genome shotgun sequence.
ACCESSION   DASUEX010000289 DASUEX010000000
VERSION     DASUEX010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3738
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1639709"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            148..750
                     /locus_tag="VFV61_04850"
     CDS             148..750
                     /locus_tag="VFV61_04850"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX4899838.1"
                     /translation="MGAVRALAANEGAELIRRCRAGDGAAWEEVVQSYSRRIYNLAYR
                     FTSRADAAEDLTQEVFVRVYRSLDQYDPKQGDLQNWLMRLARNLIIDDYRKRQRTPHD
                     YHADDLSDHTYHLHTSSGSAQEEMERRELGAQVQSGIDKLSPDLRTCVILRDIEELSY
                     QEIVTLLQIPEGTVKSRINRGRIELAKILRRMKVVAMSPV"
     gene            782..1474
                     /locus_tag="VFV61_04855"
     CDS             782..1474
                     /locus_tag="VFV61_04855"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX4899839.1"
                     /translation="MLCEDFENRLSDYLETALDAETHRAFSEHALRCPVCHQTLSEVK
                     STMQACRVAEVPPPTRELEALILQSTMPETAMTCNEFEESLTDYLDGFLPAPFYHRWE
                     RHAALCDRCTELPGEVVRSIGACYTYITEEQPIPFGLNQRILQATLGTLTAAEVRAPL
                     SARFASWLRLLLDPIVSPQLATVATMLLLAVFVLANTVSADGSISGVYQASLRLAEKS
                     YEHGLKELVGGQ"
     gene            1506..2270
                     /locus_tag="VFV61_04860"
     CDS             1506..2270
                     /locus_tag="VFV61_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899840.1"
                     /translation="MMNCTYHQKTPSVVQCNRCARPLCPACDHRIRGFPFCQDCIVTG
                     VELLRQQSPVNSAGVVRRKSSPFVATLLSFVPGLGAAYNGQTSKAIVHFAIFASFFQM
                     AVLTEGVLLFLLGVIGTWLFAAVDACRTAQLIRAGLSPEAEEDVITQRLYGNPFAWGV
                     TLIIIGTIFLLHTLLRVQLPVRELLPVALVALGAYMVFDYARRRRNSDRVVRFDSRRP
                     PPSVVTGTLDTQRYRTGETTQLSRRQIEPVSFERRS"
     gene            2294..2521
                     /locus_tag="VFV61_04865"
     CDS             2294..2521
                     /locus_tag="VFV61_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899841.1"
                     /translation="MSVLLGIFAKLSGIAALLLLVVALLGKILAAAGLLLAAIKLTIV
                     AIFIALIVLIGIAIFVDRSRRRREARNFSQV"
     gene            2817..3668
                     /locus_tag="VFV61_04870"
     CDS             2817..3668
                     /locus_tag="VFV61_04870"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX4899842.1"
                     /translation="MPRDDRFYAFIIAHTSRSRSQIRRFYVGKKWLKGFSVAALLLFF
                     GCLYGFYGLTQQALHLRIEHENQRLRAENERQQRELNHLSNRVEAVEDTSRKLAEKSG
                     VIAENASTTGAGGPAQPVEAESLVELESKMNRIEQDLRAYEAEIGRNSHTPSIWPVAG
                     SLESGFGGRRNPFGGSSYEFHSGQDIEAEWGAPVIAGASGTVTFSGWQNGYGQLVVVD
                     HGGGLTTRYGHLSAIAASVGQLLQRGEFLGRVGSTGRSTGPHLHYEVRINDQPVNPLQ
                     YLYPSSH"
BASE COUNT          806 a          978 c         1044 g          910 t
ORIGIN      
        1 aaacaggcca atcgcctgca gccgaattga gtcgggttgg ctcgccatcg gaactttcat
       61 gccaaaatca tcgtaaccgc tctcgacagg gacgttctcc tgtcggtagt tgagaccagg
      121 cgcacgtcag catcggagaa aatatcagtg ggggctgtaa gggctttggc cgccaacgaa
      181 ggagctgaac ttattcgcag atgtcgcgct ggtgacggcg cggcctggga agaggttgtg
      241 cagagttact cacggcggat ctataaccta gcctatcgct tcacttcgcg cgcggacgcc
      301 gcggaagatc tgactcagga ggttttcgtt cgcgtttacc ggtcgctgga tcagtacgat
      361 cctaagcaag gtgatctgca gaactggctg atgcgactcg cgcgcaatct aatcatcgac
      421 gattaccgca agcgccaacg cacgcctcac gattatcacg cggatgatct ttccgaccac
      481 acctatcact tgcacacctc cagcggttcg gctcaggagg agatggaacg acgcgagctg
      541 ggggcccagg tacaaagcgg aatcgataag ctatcaccgg atctgcgcac ctgcgttatt
      601 ttgcgcgata ttgaagaatt gagttaccag gagattgtca cgctcttgca aatccctgag
      661 ggcactgtta agtcccgaat taatcgcggc cgaatcgagt tggcaaagat actgcgacgg
      721 atgaaagtag tggcaatgag ccctgtttag aaaaaacgaa gagggcggaa gagagaaaca
      781 aatgctctgt gaggattttg aaaaccggct cagtgattac ctggaaacag cgctcgacgc
      841 tgaaacgcat cgtgcgtttt cggagcatgc tttgcgctgc ccggtctgtc accaaacgtt
      901 gagcgaggtt aaaagtacga tgcaggcttg ccgagtcgct gaggtccctc cgccgacgcg
      961 cgagctcgaa gcgctaatcc tgcaaagcac catgccggaa acggcgatga cctgtaacga
     1021 gtttgaagaa tcactaaccg attatctgga cggatttcta ccggcgcctt tttaccatcg
     1081 ctgggagcgt cacgccgcgt tgtgcgaccg ctgcactgaa cttcccggag aagttgtgcg
     1141 ctcaattggc gcttgttaca cctatatcac agaagaacag ccgataccgt ttggactcaa
     1201 tcaaaggata ttgcaagcga cactcggaac ccttacggcg gccgaagttc gcgcgccgtt
     1261 gtcagcgaga ttcgcttcgt ggctccgcct gttgctggat ccaattgtct cgccgcaact
     1321 ggcgacggtt gccaccatgt tgctgctcgc agtctttgtc cttgcgaata cggtttcggc
     1381 cgatgggtcg atttccggcg tttatcaagc aagtttgcgt ctggcagaaa aaagttacga
     1441 gcacggattg aaagagttgg tcggtggaca atgacacgtg gttgggtagt actgagcttg
     1501 aagcaatgat gaattgtacc tatcaccaga agactccatc agtggttcag tgcaaccgtt
     1561 gcgcccgccc actctgtcct gcgtgcgatc accggatccg cggttttccg ttttgtcagg
     1621 actgcatcgt caccggtgtc gaactgctgc gccagcagtc gccagttaac tcagccggtg
     1681 tggtaagacg aaagagctcg ccttttgtcg ccacactcct gtcgtttgtt ccaggtttgg
     1741 gggcggccta caacggccaa acttcaaagg caattgttca tttcgccatc ttcgccagct
     1801 tctttcagat ggccgtgctg actgaagggg ttcttttatt cctgcttggc gtaatcggca
     1861 cctggttatt cgcagcagtt gatgcctgtc gtaccgctca attgattcgg gcgggccttt
     1921 ccccggaagc cgaggaggac gtgatcacgc aacggcttta tgggaatcca ttcgcctggg
     1981 gggtgacgct tatcatcatc ggcaccatct ttttgctaca tacactgctg cgcgtgcagt
     2041 tgcctgtgcg tgaactgctt cctgttgcgc ttgttgcgct tggggcttac atggtttttg
     2101 actacgcaag acgacgccgg aactccgatc gcgttgtccg tttcgattca cgccggccgc
     2161 caccgtccgt agttaccgga acgctggata cgcagcgcta tcgcaccggt gagacaacgc
     2221 agctcagccg taggcaaatt gaacccgtct ctttcgaacg aaggtcttag gttttgattt
     2281 ttaggaatta agaatgtctg tacttttggg aatctttgca aaactaagcg gaatcgctgc
     2341 cctgctgctc ctggtcgtgg cgttgctggg gaaaatcctc gctgcggcgg gactgctgct
     2401 ggctgcaatc aaactcacca ttgtcgcgat ttttatcgcc ctcattgtcc taatcggcat
     2461 agccatcttt gtcgatcggt cgcgtcgccg acgcgaggcc agaaatttta gccaggttta
     2521 gtttgcctcc atagaatccg gaaactgccg ttccgcaggc ccaccttcca gaattacaaa
     2581 accctcagga tttgcaacgg ttgtccgccg tgtgataggg tcacgaatcg attgcagcac
     2641 cggccggatg gttgtccttc tcaaagcgga agttcagatc ctccgctgca aaacttcaag
     2701 caggtcagta gtacccgtaa atatattaac tagtgcgctt ctgcccctgc tggcagttgt
     2761 gctttccgtt tgttgcgtag cctcgacctc tgcgcgttca atgtggagac ttagagatgc
     2821 cccgtgacga ccgtttttat gctttcataa ttgcccatac atcccgatcc cgttctcaaa
     2881 ttcgtaggtt ctatgttggt aaaaagtggc tgaagggttt ttcggttgcc gcccttttac
     2941 tcttctttgg ctgcctttat ggattctacg gcctgacgca gcaagctttg catttgcgaa
     3001 tagagcatga gaatcaacgg ttacgcgccg aaaatgaacg tcagcaacgc gaactcaacc
     3061 acctcagcaa ccgcgtcgaa gccgttgaag acacgtcgcg caagcttgca gaaaagtcgg
     3121 gagtaatcgc cgagaacgcg tctactacgg gagcaggggg tccagcgcag ccagttgaag
     3181 cggagagttt ggtcgagctg gaaagcaaga tgaaccggat cgagcaggac ctgcgtgctt
     3241 atgaagcgga aatcggaagg aacagtcaca cgccatcaat ttggccggtc gccggatcgc
     3301 ttgagagtgg gtttggtggc aggcgcaatc cgtttggcgg cagctcttat gaatttcact
     3361 cgggtcagga cattgaggct gaatggggtg cacctgtgat tgctggagcg agtggcactg
     3421 tgacgttctc cggctggcag aatggctacg gtcagcttgt ggtcgtcgat catggcggcg
     3481 gtctcaccac gcgttacggc catctctccg caattgccgc atcagtcggt cagttgctgc
     3541 aacgtgggga attcctcggg cgggttggat ccaccggtcg ctcgacgggg ccacacttgc
     3601 attatgaagt gcgtatcaat gatcagccgg ttaaccctct gcaatatctc tacccgagtt
     3661 cacattgagc gtaggatttg ggtgtagctg agtaggcgcg actgtaaacc tagcagggtt
     3721 taggtttatg cttttatt
//
LOCUS       DASUEX010000290         2060 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_628742, whole genome shotgun sequence.
ACCESSION   DASUEX010000290 DASUEX010000000
VERSION     DASUEX010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_628742"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /gene="rplT"
                     /locus_tag="VFV61_04875"
     CDS             complement(<1..306)
                     /gene="rplT"
                     /locus_tag="VFV61_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002639666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEX4899843.1"
                     /translation="MPRAKRGNKRLERRKKILKLAKGYRGTKSKLYRSAKESVERGLN
                     FAYTGRKLKKRDFRSLWIVRIGAAARLNGINYSQLMHGLKLAGVELDRKVLADLAVNQ
                     "
     gene            complement(403..606)
                     /gene="rpmI"
                     /locus_tag="VFV61_04880"
     CDS             complement(403..606)
                     /gene="rpmI"
                     /locus_tag="VFV61_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEX4899844.1"
                     /translation="MPKLKTHKGAAKRFRLTATGKVKRGHSHARHILTKKSHKRKRLL
                     DIDALVSKSDQKRVDAMLPYGRD"
     gene            complement(681..1238)
                     /gene="infC"
                     /locus_tag="VFV61_04885"
     CDS             complement(681..1238)
                     /gene="infC"
                     /locus_tag="VFV61_04885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEX4899845.1"
                     /translation="MRINERIRVPEVRVIADEGEQLGVMDIRDAIRTARERGLDLVEV
                     APTADPPVCRIIDFGKFQYEAKKKANEAKKKQVTITVKEVKFRPGTDDHDYGYRMKHA
                     REWLEEGDKVKATIWFRGREMTHRELGARILEKLERDLADVGEVEARPRMEGNQMFII
                     FGPKRHKGVTKPEHPPAPGRAEPEQ"
     gene            complement(1385..>2060)
                     /locus_tag="VFV61_04890"
     CDS             complement(1385..>2060)
                     /locus_tag="VFV61_04890"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF015113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEX4899846.1"
                     /translation="SVRGKDTGTHYLGSDEDWNNAEGALIDALKQRGISFERIEGEAV
                     FYGPKIDIRVKDAIGRSWQLSTVQFDFNFPERFELEYIGEDNKPHRPIMIHRALFGSL
                     ERFFGVLIEHYAGAFPLWLAPVQVAVLPITDRVNEYAERVAAELKQHNIRVEMNLRSE
                     KIGAKIRDAQMQKVPFMLVLGDREMEQGQVAVRERAKGDLGVVPLEEFINMARKLIET
                     RAINNS"
BASE COUNT          405 a          626 c          495 g          534 t
ORIGIN      
        1 ctgattgacc gcgagatcgg ctaaaacctt tcgatccagc tcaaccccgg caagcttcag
       61 cccgtgcata agctgtgagt agttgattcc atttaatctg gcagcagctc ctatgcgcac
      121 aatccagagg ctgcgaaaat cacgcttctt cagctttcgt ccggtgtagg caaagttcag
      181 cccacgttcc accgactctt ttgcagaacg atatagtttc gactttgtgc cgcggtaccc
      241 cttggccagc ttcaaaatct tcttgcgcct ttcgaggcgc ttgtttccac gctttgctct
      301 aggcatattt tatttccctt cctggaggtt caagctagca atgatggttt cgacggtctc
      361 ccacatccac cgcttgccgg ccctgcggcc tccgcctttc aattagtctc gaccatacgg
      421 gagcatcgca tcaacgcgct tttgatcgga ttttgaaacc aacgcatcaa tgtccagcag
      481 tcgcttacgc ttgtggctct tcttggtcaa aatgtgtctc gcgtgcgagt gcccgcgttt
      541 gactttcccc gtggcagtga ggcgaaaccg cttcgctgcg cccttatgtg ttttcagttt
      601 tggcataact tctccgctaa tagatttcca atcggcaatt gccaattgac agttgtgtca
      661 acacgggtcc cgcccgcttt ctactgctct ggctcagctc tgccaggtgc cggcgggtgc
      721 tccggcttcg tcacaccttt gtggcgcttt ggcccaaaga tgatgaacat ctgattacct
      781 tccatccgag gcctcgcctc gacttcgccc acgtccgcca gatcgcgttc cagcttttcc
      841 agaatgcgag ccccaagttc gcggtgcgtc atttcgcggc cacgaaacca gatcgtggct
      901 ttgaccttgt cgccctcctc aagccattcg cgggcgtgtt tcattcgata gccataatca
      961 tggtcgtcgg tgccggggcg aaacttaacc tccttgactg tgatcgtgac ctgtttcttc
     1021 tttgcttcgt ttgccttctt cttggcctcg tactgaaatt taccgaagtc gatgatgcgg
     1081 caaaccgggg ggtctgcggt gggggccacc tcaaccagat ccaaccctct ctcccgcgcg
     1141 gtgcgaatgg cgtcccggat atccattact ccgagctgct cgccctcgtc cgcgattacg
     1201 cgcacttccg gtacgcgaat tctttcgtta atacgcacgg ccggcaaacg acgattatca
     1261 ggacgctggc cgcgacctcg aaagcctgta gctattgctg cacctcctcc atcaaggttc
     1321 cgtgttttga ctttcggttg cgagttgaaa cacccactcg aaaccgaaaa ctcctaactc
     1381 gaaactagct gttgttgatt gcgcgcgtct cgatcagttt gcgcgccatg ttaatgaact
     1441 cttccagcgg gactactccg agatcgccct tagcgcgttc tctcactgcc acctgccctt
     1501 gttccatttc acggtcgcca aggacgagca taaaggggac cttttgcatc tgcgcatcac
     1561 gaatcttcgc gccaatcttc tcgctgcgca gattcatttc aacgcggatg ttgtgctgct
     1621 ttagttcagc cgccacgcgt tccgcatact cgttgacgcg atcggtaatg ggtaatacgg
     1681 caacctgcac cggggccaac caaagcggaa aggcgccggc ataatgttcg atcagcactc
     1741 caaagaaacg ctccagcgag ccaaacagag cgcgatggat catgatgggc cgatgaggct
     1801 tgttgtcttc gccaatgtat tccagctcaa atcgttctgg aaaattaaaa tcaaactgca
     1861 cagtactcaa ctgccatgaa cgtccaatcg cgtccttgac gcggatgtca atttttgggc
     1921 cgtagaagac ggcctcacct tcaatgcgtt cgaaggagat cccgcgttgc ttcaaagcgt
     1981 cgatgagcgc gccctcagcg ttgttccagt cttcatctga ccccaggtaa tgggtgcccg
     2041 tatcctttcc ccggaccgac
//
LOCUS       DASUEX010000291         4450 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_274479, whole genome shotgun sequence.
ACCESSION   DASUEX010000291 DASUEX010000000
VERSION     DASUEX010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4450
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274479"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1670)
                     /locus_tag="VFV61_04895"
     CDS             complement(<1..1670)
                     /locus_tag="VFV61_04895"
                     /inference="COORDINATES: protein motif:HMM:NF024298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HEX4899847.1"
                     /translation="MKRTRPKKLLVIGLLLGFVCADCASRMSDRQSNAVLAAPGTESV
                     DQSSQPRLVVQAGPDNAVNGIAFSPDGKLIATAGGERTVKLWDAATATELRSFLGHDD
                     AVFAVAFSPSAKLIASGSYDGTVKLWDTATGTEIRSLDAHALGVYAVAFSPDGKVIAS
                     GGVEDTRDDQEPPHAIKFWDAATGQLLQSVERESEVRSIAFSPDGKAIASDSWGNNSV
                     VLWDTQSGKKIRTLQSKASELEAVAFSPDGKLIAGGVGAHRIELWDAATGNLLRTLEA
                     KPEGLDQGRCEAEANTATSIVITFSPDSRSIVSGACDHTTKLWDVATGQQKYSWNGHS
                     GGVVSVAFSPDGKAIASGSLDQTVKVWDIASTKELHSFASHATEIQSVAISPDGRIIA
                     RGSTDGKVKLWDTATRKPLQSLEGHVYRINALAFSNDGRIVASGGADNKILLWNVATG
                     RLLRSLIAGPIDGASESAPRLTSLAFSPDGKLIASAGNSYRIRLWNTATGKELHSPIG
                     ESSGGGMTSVEKVAFSPDGKALAASYDESSLILWNVASGRQLISIEDA"
     gene            complement(1710..3953)
                     /locus_tag="VFV61_04900"
     CDS             complement(1710..3953)
                     /locus_tag="VFV61_04900"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HEX4899848.1"
                     /translation="MRSPITFILVLIALVLSGPAPQLFARATQNPSSEPRQSTTQNAT
                     PRKRALLVGVSKYCRGDGSECGKRKKYWWDLNSEPDLEALKQVLLQKFGFTESEIKVL
                     KTSEETTHDSIRRTFKSFLIDQTRPGDTVYFHYSGHGGTVPDDDQHGPNPLVGDEISG
                     YDQTLIPSDYVSRQEATNDIRDDEIKKWLDELKEKKPGNVTITMDSCFSGTNTRGGRF
                     LVRGSKWDGAAPQLQKNRGSKPTAEDSTGLLPQAYAASLGYVVIAATRNDQLAGETDG
                     FDGKAMGLLSYALAKALMTAGPRTTYRDIFGRIYDEVTRDGHDQNPTIEGEMDKVLMQ
                     GIALPPQPFINVGIENEEVLLKAGYLQGMTKGSRFDLFSADTKDFKQATPIASAGIER
                     VDAVSSRLKLIAPAKLDLKQLQNARARETDHLFDDARLRVAFEGIAQPAASPDDLATR
                     VRQMDIVRGDLKAANEWDIKICRGKCTDEKPSALGNDENFTGGYTMMRQDGSIVRRVP
                     DEADAFTKISLALKGEVRWRFVKNLKNDDSTVKIEMRLVPVTAEERSSLTVGDRRIQV
                     ISKTKDLGAPLEVGALHSLPEGSYYLMEIKNSGYSDAYINVIDLTSDGHIAPLWPNPV
                     IPLGNADENKIPADGEWHRVPAPFVVAITPPFGPEIFKVIATLEPADFSPLLSERFVG
                     NKRGGNDRGAKEEQTMLGRLLKTATTIGMTTRGEIEGTQNISSPNAGTSGWATFEFQF
                     VAVTKAA"
     gene            complement(3997..>4450)
                     /locus_tag="VFV61_04905"
     CDS             complement(3997..>4450)
                     /locus_tag="VFV61_04905"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX4899849.1"
                     /translation="VDGTQTLYNMKTGTISDYSSPGFRRDNIVKPEIAAPATYTISPL
                     SKDSTSMGRNQDGTPNKANITDDGYHLAWAGTSASSPYTAGVIALMLQKNPTLTAVQV
                     KEILKKTAIKDELTGATPNPFWGYGKLNPAAAIRDTTPLAGPPRRAGQ"
BASE COUNT          914 a         1253 c         1183 g         1100 t
ORIGIN      
        1 ctcgcgtctt cgatggagat caactgccgt cccgaggcga cattccagag tatgagagat
       61 gattcatcgt aacttgctgc aagcgcttta ccgtcgggac taaacgcaac tttctcgact
      121 gacgtcatgc cgccacccga actctcgcct ataggagagt gcagttcctt gccggtggca
      181 gtattccaaa gtctgatgcg gtaactgtta ccggcactgg cgatcaattt tccatccgga
      241 ctgaaagcaa gtgacgtcag tctgggtgct gattcagatg cgccatcaat tggtcctgct
      301 attagagaac gaagcaagcg accagtcgct acgttccaaa gcaggatctt gttatcggcg
      361 ccgccgctcg cgacgattct tccgtcgtta gaaaaagcga gagcgttgat ccggtagacg
      421 tgaccctcaa gcgattgcag aggtttgcgg gtcgcagtat cccagagctt aacctttccg
      481 tcggtactgc ctcgggcaat gatgcgccca tcggggctga ttgcaaccga ctgaatctcg
      541 gttgcgtggc tggcaaatga atgcagttcc ttagtgctgg cgatgtccca taccttcact
      601 gtttggtcga ggctcccgct tgcgatcgcc ttaccgtccg ggctgaaagc aaccgacaca
      661 accccaccgg aatgaccatt ccaggaatat ttctgttgac ctgtggcaac gtcccaaagc
      721 ttggtggtgt ggtcacacgc gccgctaacg attgatctgc tatcggggct gaaggtgatt
      781 acaatagacg ttgctgtatt ggcttcggct tcgcagcgcc cttgatccaa gccttccggt
      841 ttagcttcga gcgtccgcag caagttgccc gtcgcggcat cccacaactc aatcctatga
      901 gcaccaacgc cgccggcgat caactttccg tccggactaa aagcaacagc ctccagctct
      961 gacgccttgc tctgcaatgt gcggatcttt ttgcctgact gggtgtccca gagcacgact
     1021 gagttgttac cccaactgtc actcgcgatt gcctttccat cgggactgaa ggcaatggag
     1081 cgaacttcgc tttcacgctc cactgattgc agcaactgac ccgttgcagc atcccaaaac
     1141 ttgatggcgt gcggtggctc ctgatcatca cgcgtatctt ctacgccgcc gctggcaatt
     1201 acctttccgt cgggactaaa ggcaactgca tagactccca gcgcatgcgc gtctagtgat
     1261 cgtatttccg tccctgtcgc ggtgtcccat agtttgacgg tgccgtcata actgccgctg
     1321 gcgatcagtt ttgcactcgg gctgaaagcg acagcaaaga ccgcgtcgtc gtgacccaga
     1381 aaggaacgga gttctgtagc ggtggctgcg tcccatagtt tgaccgttcg ctcgccaccc
     1441 gccgttgcga tcagcttacc gtcaggacta aacgcaatcc cgttgacggc gttgtcagga
     1501 cccgcttgaa ccaccagccg gggctgacta ctctggtcaa ctgattcagt acctggtgct
     1561 gccagcaccg cgttggactg acgatcggac atgcgcgaag cgcagtcagc gcagacaaag
     1621 cccagcagca acccgatgac taatagtttt ttgggccgag tccttttcat ctaatcctca
     1681 cctgagttct ttccgatcac tctttagcat caagctgcct ttgtcacagc gacaaactgg
     1741 aactcaaaag ttgcccagcc cgaagtaccc gcgttagggg acgagatgtt ctgcgttcct
     1801 tcgatttcgc cgcgcgtagt cattccaata gttgtcgcag tttttaataa ccgccccagc
     1861 atggtttgct cttctttggc cccgcgatcg tttccccctc gtttgttacc cacaaacctc
     1921 tcacttagca gcgggctgaa atcggcaggc tcgagcgtgg cgattacctt gaaaatctcc
     1981 ggtccgaaag gcggcgtgat cgctacgaca aacggcgcgg gaacacgatg ccactctccg
     2041 tcggccggaa tcttgttttc atctgcgtta cccaagggga ttacgggatt cggccaaagc
     2101 ggagctatat gtccgtcgct cgtgagatcg ataacgttga tgtaagcgtc ggaatagccg
     2161 ctgtttttta tttccatcaa gtagtaggag ccttccggta gagaatgcag ggcaccaact
     2221 tccaacggag ctcccagatc tttcgtcttg cttatcacct gaattcgccg atctccaacc
     2281 gtgagacttg aacgctcttc agcggtgacc ggaaccaggc gcatttcgat tttgacggtt
     2341 gaatcgtcgt tcttaagatt tttgacgaag cgccagcgga cttcaccttt cagtgccagc
     2401 gaaatttttg tgaacgcgtc tgcttcgtca ggcacacgtc gaacaatgga gccgtcctgc
     2461 cgcatcatgg tgtagccgcc ggtaaagttt tcgtcatttc ccagcgctga gggcttttcg
     2521 tcggtgcatt tgccacgaca gattttgatg tcccattcgt ttgccgcttt gaggtcgccg
     2581 cggacgatat ccatttgtct gaccctggtg gcgagatcgt ccggtgatgc tgcgggctgt
     2641 gcgattccct cgaaggcgac acgcaaacgc gcgtcatcaa acaggtggtc ggtttccctg
     2701 gcgcgtgcgt tttgcagttg tttcaaatcg agcttcgcgg gagcaatcaa tttcaaccgc
     2761 gaagaaacag catcaactct ttcaatcccc gcgctggcaa tcggcgtcgc ttgtttgaag
     2821 tctttcgtgt ccgcggaaaa cagatcaaag cgcgagccct tcgtcatccc ctgtaagtat
     2881 ccggccttca gcagcacttc ctcgttttca attccgacgt taatgaaggg ctgcggaggg
     2941 agggcaatgc cttgcattag caccttgtcc atctcgcctt cgattgtcgg attctgatcg
     3001 tgcccatcgc gcgtcacctc atcgtaaatt cgtccgaaga tgtcgcgata agtagtgcgg
     3061 ggaccggcgg tcatcaatgc cttggccaag gcgtaggaga gcaatcccat cgccttgcca
     3121 tcaaatccat ccgtctcacc agccagttgg tcattgcgtg tcgccgcaat cactacgtaa
     3181 ccgagcgatg cggcgtaggc ttgcggtaac aaaccagttg agtcttccgc agtgggcttg
     3241 ctaccgcgat tcttctgaag ttggggtgcc gctccgtccc atttgctacc acgcaccagg
     3301 aaccttcctc cgcgagtgtt ggtgccggaa aagcaactat ccatggtgat cgtaacgttg
     3361 cccggcttct tctctttcaa ctcgtcaagc cactttttga tctcgtcatc gcggatgtcg
     3421 ttcgtggctt cctgccgcga gacatagtcg gagggaatca gcgtctggtc ataaccactg
     3481 atttcatccc cgacgagggg attaggcccg tgctgatcgt cgtcgggaac ggtgccgccg
     3541 tgtccggaat aatgaaagta aaccgtgtcg ccggggcggg tttgatcgat caaaaaggac
     3601 ttgaaggtac ggcggattga gtcgtgggtg gtctcttcgc tggttttcag gaccttgatt
     3661 tcgctttctg tgaaaccaaa cttctgcagc agcacttgct tcaacgcctc gaggtctggt
     3721 tctgagttca gatcccacca gtattttttt cgcttgccgc attcactacc gtcgccacgg
     3781 caatatttcg aaacgccaac gagcaatgcg cgcttgcgcg gagttgcgtt ctgagttgta
     3841 ctttgccgag gttcagagga tgggttttga gtggcccgag cgaataactg cggggcagga
     3901 ccactcaaaa cgagcgcaat taagacaaga atgaacgtaa tgggactacg catggccacc
     3961 ctctccttcg attacaactc tcaagcgagg ttcgacctac tgcccagcac gccgcggcgg
     4021 gccagccaat ggcgttgtgt ccctgatagc agcggcggga tttagttttc cgtaacccca
     4081 aaagggattt ggcgtagcgc ccgttagctc atccttgatc gcagtcttct tgagaatctc
     4141 tttgacttga accgcggtga gagtgggatt cttttgcagc atcaacgcaa tcactccggc
     4201 cgtgtaagga gaagaggcgc tggtgcccgc ccaggctaaa tggtaaccat catcagtgat
     4261 gttggcctta ttcggagtgc catcctgatt ccttcccatc gaagtggaat cctttgatag
     4321 cggcgagatc gtgtaagtgg caggcgcggc aatctccggt ttcacgatgt tgtcgcggcg
     4381 gaatccagga ctggaataat ccgagatggt gcccgtcttc atgttgtaga gagtttgcgt
     4441 gccgtcgacg
//
LOCUS       DASUEX010000292         3325 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_59885, whole genome shotgun sequence.
ACCESSION   DASUEX010000292 DASUEX010000000
VERSION     DASUEX010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3325
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59885"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1115)
                     /locus_tag="VFV61_04910"
     CDS             complement(<1..1115)
                     /locus_tag="VFV61_04910"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4899850.1"
                     /translation="MASLTYLQLLRSNNYFRRLWLGQVISELGNWFNFIAALGLVRAV
                     SHGAPEVTTIMLVTRLLPFTIFAPLAGAFVDRWSRRTVMIVSDLARVLVALTMLLVRQ
                     PEDLWIAYVCTVVLSFFGAFFEAAKNAAVPNITGDRDLLAGNALMFSSRFLLMAFGAA
                     LGGWTAATVGYRAAFVVNAISFAASAYSVWLIPDAQTRTAANIETVPRAARRGYWSDI
                     REGWSFILSHAPVAAILAINILWATGGGSINLISDRLGGIVFAGQGGISGDEAVAVLY
                     FAAGVGLFLGMMIARRLGTQLEVMHATIPFIGWALIVQGVVFAFIGLAPSLWLACLLI
                     FASRVLLAAEFAIQETLLMRLVPDHLRGRVGTSDRAA"
     gene            complement(1324..2118)
                     /locus_tag="VFV61_04915"
     CDS             complement(1324..2118)
                     /locus_tag="VFV61_04915"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX4899851.1"
                     /translation="MNYETIQFEVRGGVAVITLNRPEALNALTLAVAAEFKAAVAEAV
                     AQGSRAVVLTGAGRAFCAGGDLREMQKIASQNGRVEAFFDEPLRELNDCIMLIRRTPL
                     PFIAAVNGSAAGGGCNLALACDLVIAGEGARFNQAFVKIGLTPDCGGTFILPRLVGWR
                     RAMQLMMTGEVVTAPQALAMGMINAVVPDDQLIAQAMEMAASLAQSPTVAIGRIKELL
                     EASAKNDYGEQLELERETQLKSGMTKDFKEGVAAFIEKRPAKFVGG"
     gene            complement(2303..2596)
                     /locus_tag="VFV61_04920"
     CDS             complement(2303..2596)
                     /locus_tag="VFV61_04920"
                     /inference="COORDINATES: protein motif:HMM:NF024843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CxxC-x17-CxxC domain-containing protein"
                     /protein_id="HEX4899852.1"
                     /translation="MPDLEITCAECATPFAFTEREQEYYRERNLTHPKRCKPCRDARR
                     ANFGGSRGPGGERERFEITCDQCGKADTVPFKPSSGRPVLCGECFSANRAKDQ"
     gene            complement(2910..>3325)
                     /locus_tag="VFV61_04925"
     CDS             complement(2910..>3325)
                     /locus_tag="VFV61_04925"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899853.1"
                     /translation="VFFFSMHQYPWYPGTGSRGETGVIRGRGYTLNVPLKAKTPAVEQ
                     KRSFEAALDEIDSSFHPDLILISAGFDSHAGDPLGQLLLKDEDFIDMTRTVKQWAAAT
                     CAGRLVSCLEGGYNLQTLGETVRAHVAELTRREKP"
BASE COUNT          767 a          968 c          888 g          702 t
ORIGIN      
        1 tcggcagccc ggtcggacgt accaacgcgc ccgcgcaagt gatcggggac aaggcgcatc
       61 agcagcgtct cctggattgc aaattccgcc gccaacagaa cccgcgaggc gaagatcagc
      121 agacaggcga gccaaagcga aggagcgagg ccgatgaacg cgaagaccac accttgaaca
      181 atcagggccc aaccgataaa cggaatggtc gcatgcatca cctcgagctg cgtgcccaac
      241 cggcgcgcaa tcatcatccc gagaaagaga ccaacgccgg cggcaaaata gagcactgct
      301 accgcctcgt cgccgctgat ccctccctgt cccgcgaaga cgatgccgcc gagtcgatca
      361 gaaatgagat tgattgagcc gccgcctgtc gcccagagga tattgatcgc caggatggca
      421 gccaccggtg cgtgactgag gatgaaagac cacccttcgc gaatgtcaga ccagtatccc
      481 cgtctcgctg cgcgcggcac ggtttcgatg tttgcggccg ttctggtttg cgcgtcggga
      541 atcaaccaaa ctgaataggc agaagcagcg aatgagattg cgttaaccac aaaggcagct
      601 cgataaccaa cggtggcagc agtccagccc cccagagcgg cgccaaaagc catcagcagg
      661 aaccgcgacg aaaacatcaa ggcgttaccg gcgagtagat cgcgatcgcc ggtgatgttg
      721 ggtactgccg catttttggc ggcttcaaaa aaagcgccaa agaaggaaag aaccacggta
      781 cagacgtaag cgatccaaag atcttccggc tgtctcacca acaacattgt cagcgcaacc
      841 agtactcgcg ccagatctga gacgatcatt acggtgcgtc tcgaccaacg atcaacaaag
      901 gctccggcca atggcgcgaa gatggtgaac ggcagcaggc gcgtcaccag catgatggtc
      961 gtgacttcgg gtgcgccgtg tgagacagcg cgcaccaatc ccaacgcggc aatgaagttg
     1021 aaccagttgc cgagctcaga aatcacctgc cccagccaca atcgtctgaa gtaattgttg
     1081 ctgcgcagaa gttgcagata ggttagtgac gccatccgaa ttcttcgttt tggaactttg
     1141 aactttgaac tttttgcttt gatgctcccg gaacttgagt aagtccggcg aacaacttaa
     1201 acaacaccat tcagttgagc tcaaagcacc aagtacaaag ttcaaagtac aaagctcaaa
     1261 ggtcaaataa taaagccgcc aacccaggtt agcggctcgc gactttcgag ttctttctct
     1321 taactagcca ccaacgaatt ttgctggccg cttctcgatg aaagccgcga cgccttcctt
     1381 gaaatctttt gtcattcctg atttaagttg cgtttcccgc tccagctcca gttgttcacc
     1441 gtaatcattt tttgcactag cctccagaag ctccttgatg cggccgatgg cgactgtggg
     1501 cgactgcgcc aaactcgccg ccatctccat cgcctgggcg attaattgat cgtcaggaac
     1561 gactgcgttg atcatgccca ttgccaaagc ctgcggcgcc gtcactactt ctccagtcat
     1621 catcaattgc atggcccgcc gccatcctac caaacgcggc aggatgaaag tccccccaca
     1681 atcgggtgtc agaccaatct tcacgaaggc ctgattgaaa cgcgcgccct cgccggctat
     1741 tacgaggtcg caggcaagcg cgagattgca cccgcccccg gcagcggagc catttaccgc
     1801 ggcgataaac ggaagtggag tgcggcgaat cagcatgatg caatcattca gctcacggag
     1861 cggttcgtca aaaaaggcct caacgcgacc gttctgagag gcgattttct gcatctctcg
     1921 cagatcgccg ccggcgcaaa acgcccgtcc ggcgcctgtc aaaacaacag cgcgacttcc
     1981 ttgtgcgact gcttcagcaa ccgctgcttt gaattcggcg gccaccgcca acgtcaaggc
     2041 gtttagcgcc tcaggacggt tgagcgttat caccgccacg ccgccgcgca cttcgaattg
     2101 aatggtttcg taattcatcg caagtagtca gaagtcaata gtcagtagtc agtggttcgt
     2161 ggttcgtggt tcgtggtcag tggtcagtgg tgtggtcagt ctgttaacag gtgacagctg
     2221 acaagtaact actgcctcct gtcccctgtc ccctgtctcc tgtctactgc ctaccgtcta
     2281 ctgcctgccc ttccctactt cctcactgat ctttcgcgcg attcgcgctg aagcattcac
     2341 cgcagagcac cgggcgtccg ctggagggtt tgaagggaac ggtgtcagcc ttgccacatt
     2401 gatcgcaagt aatctcgaat cgctcacgtt ctccgccggg cccccttgaa ccgccaaaat
     2461 ttgcgcgccg cgcgtctcga caaggcttac agcgcttagg atgagttaag tttctttctc
     2521 gatagtactc ctgttcgcgc tcggtgaacg cgaacggggt ggcgcattcg gcgcaggtga
     2581 tttccagatc tggcatgggg cgtttctcca tgagagcgag accgagcagt ctgctctctc
     2641 agctcttcga ggatcgcgtt gacttagggt gcgctgtcaa ctcttacagg gacgatcact
     2701 gccgaagcgg ttttaggtgt ggcagagttt actccagcgg cacccggcgt tcaacaagca
     2761 gaaggcagga gcaggaggca aaaatgtttt ccatttctca tttttcattt gacgggctca
     2821 ctggtcgctt tgtttgtcgg ctagccagct atccaaggcg aataattacg ggctcgttag
     2881 agggaaatgg aaatggtttt gcctcctgct tacggttttt cccgacgcgt caattccgcg
     2941 acgtgggccc ttactgtttc gcctagtgtc tggaggttgt aaccaccttc gaggcaagag
     3001 accaaacgcc cggcgcacgt tgccgcggcc cactgcttca ccgtccgggt catatcgatg
     3061 aagtcttcgt ctttaagcaa caattggccc agcgggtcgc cggcatggga gtcaaaaccc
     3121 gccgaaatca gaatcaggtc gggatggaag ctcgagtcaa tctcatcgag cgctgcctcg
     3181 aaggaacgct tttgttccac ggccggggtc ttagctttga gcggaacatt caaggtataa
     3241 ccgcgcccgc ggatgacgcc ggtttctcct cgcgatccag ttccgggata ccacggatat
     3301 tgatgcatcg aaaaaaagaa aacgc
//
LOCUS       DASUEX010000293         3063 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1183068, whole genome shotgun sequence.
ACCESSION   DASUEX010000293 DASUEX010000000
VERSION     DASUEX010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3063
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1183068"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="VFV61_04930"
     CDS             complement(<1..363)
                     /locus_tag="VFV61_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899854.1"
                     /translation="MTGKIKILIAYDGSACAEAALDDLQRAGLPQVAEAVVMSVTEIW
                     MPPPPPSTYQILELANAVYVPADMTRVYAKGSPAFQEAQGLADRAAARLRANFPAWEI
                     TAQASVGSARRELIRHADG"
     gene            721..>3063
                     /locus_tag="VFV61_04935"
     CDS             721..>3063
                     /locus_tag="VFV61_04935"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX4899855.1"
                     /translation="MSQQTTSASLPRGGDVVLRDGSTVSVRAMQPDDEPLLFDLFTSL
                     SEDSRWMRFFGVSKDSALAAEAHREAQVDFCRTVGLVALAGSKNQIVGHAFYASNGEN
                     RAEVAFTIANDYQGRGLASILLGQLAEIAVSNGIQVFEAEVVAANHAMLGVFRESGFR
                     LSVQADAGQLHVMFPTSLSDEAASKFERRESTAAVNALKLFFEPQAVAVIGASRKRGT
                     IAGEVFHNLISYPFRGPVYPVNPAAEVVQGVPAYPTVEDIPGPIDLAVIVVPAANVLD
                     VARSCGRKGVKALVIISAGFSETGKEGQARQAELVRVCRSAGMRLIGPNCMGILNADP
                     EVRLDATFAPHVPPPGRVGFSSQSGALGLAIIDHANSLGLGISTFVSVGNKADISGND
                     LLRYWEDDKRTDVILLYLESFGNPRKFSQIARRVGRQKPIVVVKSGRSQAGARATSSH
                     TGALIAASDVTVDALFKQAGVIRTDTLEELFDVASLLANQPLPEGRRVGIITNAGGPG
                     ILCADACEARGLEIPVLDERKQEHLRGLLPPGAGVANPVDMIASASADHYRETIKTVA
                     ADPNIDALVVIFTPPLVVRAEDVARAISEEVQELKRSKPVLAVFLSAGEAPVELRQSE
                     VRIPCYRFPETAAIALSRAARYHEWRNRPEASPVHFPEVRRDEAAALVSTALARGEDW
                     LNPTETAALFACYGLPLIDQQIAESPEEAGAIAAEMNCAVALKAIAPGVIHKSELGAV
                     KLHLKGRAQVSQVAHDMTDRLAQNGHVPTGFVVQRMAQPGV"
BASE COUNT          652 a          871 c          882 g          658 t
ORIGIN      
        1 tccgtcggca tgtcgaatca gttcgcgtct ggctgagccc accgaagctt gtgctgttat
       61 ttcccaagcc ggaaaattgg cgcgcaaccg ggccgctgcg cggtcggcga ggccttgcgc
      121 ttcctgaaat gcaggcgagc ctttcgcata aacgcgcgtc atatcggccg gcacgtacac
      181 cgcgttcgcc agttcgagaa tctggtaggt ggacggtggt ggtggcggca tccaaatctc
      241 agtaaccgac ataaccacgg cctcagcaac ctgcggcaat ccggctcgtt gcagatcgtc
      301 caaggctgcc tcagcacaag cggaaccgtc gtacgcaatc agaatcttta tcttcccagt
      361 catcatggcc tctttcgcaa aatccgactc tcaacatcag tcctttcgca acaagcattc
      421 cctgaacgta aagggcgttt cgccgtaaaa accgcggaaa agcgcctcgg atgctttgct
      481 ggttgcggag gttcacgcaa agtcgtgcga aacttttccc aaccaaattg aaccaaggcg
      541 aataaagcgc agaaccccgc aaaggtatgt accttgctcg gacgtgagtt ataaggtgat
      601 cacttcaatt gccaattggc gattggcgat tgccaattag ccctagcaag agtagccaat
      661 cggaaactgg caatcgtcaa tcggcaatcg aagcgtccag ctaccgtgcc cggaaaaatc
      721 atgagtcaac aaacaacatc tgcttccctg ccacgaggtg gcgacgtcgt actccgcgac
      781 ggctcgactg tctccgtacg cgcaatgcag ccggatgatg aaccgctcct tttcgacttg
      841 ttcacgtcgc tctccgaaga ctcgcgctgg atgcgcttct tcggtgtctc aaaagactcg
      901 gcattggccg ctgaggctca tcgcgaagcg caggttgatt tttgccgcac agtcggtttg
      961 gtagcgcttg cgggatccaa gaatcaaatc gtagggcacg cgttctacgc gtccaacggc
     1021 gagaatcgcg cggaggttgc tttcacaatt gcgaatgact atcaaggtcg cggactcgct
     1081 tcgatcctat tgggtcaact cgccgagatc gcagtgagca atggcataca ggtgttcgaa
     1141 gctgaggtcg tcgctgccaa tcatgcaatg ttgggagttt tcagagaatc cggatttcgt
     1201 ctcagcgtcc aggccgacgc cggtcagctt cacgtgatgt tcccgacctc gttgagcgat
     1261 gaagctgctt ccaaattcga acgtcgcgag agcacggctg cggttaatgc gttaaagctc
     1321 ttctttgaac cgcaagcggt ggccgtgatt ggcgcttcga gaaagcgcgg cacgattgcc
     1381 ggtgaagttt ttcataatct cattagctat ccttttcgcg gtccggtgta cccggtcaat
     1441 ccagcggctg aggtggttca gggtgtgccg gcttatccca cagtagaaga tattcccgga
     1501 ccaatcgatc tggcggtcat cgtagttcct gcggcaaacg tcttggatgt tgcgcggtcc
     1561 tgcggccgca aaggagttaa ggccctcgtc atcatctcag cagggttttc tgaaaccgga
     1621 aaagaggggc aggcgcggca ggcagagttg gtgcgagtct gtcgctcagc tggtatgcga
     1681 ttgatcggac caaactgcat gggcattctg aatgccgatc ccgaggtacg gctcgatgcg
     1741 acctttgcgc cgcacgtgcc accaccaggc cgcgtgggtt tctcttccca gagtggcgca
     1801 ctcggactgg cgattatcga tcacgcgaat tcattagggc tcggcatctc aacgtttgtt
     1861 tctgtaggca acaaggccga catatcagga aacgacctac ttcgctattg ggaagacgat
     1921 aaacgtaccg acgtgatcct cctctatctg gagtcgttcg gtaacccgcg caagttttcc
     1981 cagatcgccc gtcgggttgg ccggcagaag ccgatagttg tagtgaagag cggtcgctcg
     2041 caggccggag cgcgggccac ctcatcgcac accggggcac tgattgctgc ttccgacgta
     2101 accgtcgacg cgctgttcaa gcaggcgggt gtgattcgca ctgacacgct cgaggagtta
     2161 ttcgatgtcg cttctttact tgccaatcaa cccctcccgg agggaaggcg agtgggaatc
     2221 atcacgaacg ccggaggtcc cggtattctg tgcgccgatg cttgtgaagc acgcggttta
     2281 gaaatccctg tgctcgacga aaggaagcag gaacacctgc gcgggctttt gcctcctggt
     2341 gccggcgtgg caaatccggt cgacatgatc gcgtcagcct cggcggacca ttaccgcgaa
     2401 accattaaga ctgtcgcggc tgatccgaac attgatgccc tcgtggtgat cttcacgcca
     2461 ccactcgtgg tgcgagcaga agacgtcgca cgggcaatct cagaagaggt gcaagagctg
     2521 aagcgcagca agcctgtact ggctgtgttc ttgtccgccg gcgaggcgcc ggtcgagctg
     2581 cgacaaagcg aagtgcggat tccttgttat cgatttcccg agactgcggc gatcgctctt
     2641 tcgcgcgcgg ctcgctatca cgagtggcgc aaccgtcccg aagcctcgcc ggtgcatttc
     2701 cccgaggtgc gacgtgatga agcggcggct ctcgtatcca ccgcgcttgc ccgcggcgag
     2761 gactggctca acccaacgga aactgcggcg ctctttgctt gttatggtct ccctttgatt
     2821 gaccaacaaa tcgccgagag tccggaggag gccggagcaa ttgccgcaga aatgaactgc
     2881 gccgttgcac tgaaagcaat agctccaggc gttattcaca aaagcgaatt gggagccgta
     2941 aagcttcacc tcaaaggccg cgcacaggtg agtcaggtag cgcacgacat gacggaccgg
     3001 ctcgcacaga acggccacgt cccgaccgga ttcgtggtcc agcgtatggc acagcctgga
     3061 gtt
//
LOCUS       DASUEX010000294         2515 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1549993, whole genome shotgun sequence.
ACCESSION   DASUEX010000294 DASUEX010000000
VERSION     DASUEX010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2515
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1549993"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            65..1582
                     /locus_tag="VFV61_04940"
     CDS             65..1582
                     /locus_tag="VFV61_04940"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX4899856.1"
                     /translation="MALSRKKKIIIAVSAVAVLALVIIISVFASRKEESEVTTVKIEV
                     RPELRQTVTASGEVRPIRYIKLTSEVQGRIEEIYVNAGDIVTRGKPLVRIDPTQLQSN
                     QEAQWAAAQASMNDVQSARNAVSSAQQGLVVAEAAVSQARQQLVAVQNSVERAQVDLN
                     TAQRELKRNADLIEAGVASRSEYDAARDRFDQAKIALDTARANLESQKIAVKEAMERA
                     NQQRIAVRDARTGIKSSEMRASQQQALLRGQSSQRSKATQLSPLNGVIADIPARVGEF
                     AVAGLSTTSLMTIADMSTINVEVNVDETEISNVEVGQPVKVKVDALGEKEITAVVNQK
                     NPLAVSKSDTQGGLSARVNVQEAKEFKVTVELRDLTDEVRNALRPGMSATATITTKVK
                     NNVVAVPLEAIVEKSPNASPSPAATVAGSAPNAPAGEKPKNLKGVYVIEGSNKVRFIE
                     VTTGITGEADIEVTSGVQAGMEIVRGPSRVLKTLKDGMSVKRQVKKPGANANEAS"
     gene            1582..2343
                     /locus_tag="VFV61_04945"
     CDS             1582..2343
                     /locus_tag="VFV61_04945"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4899857.1"
                     /translation="MSDATLITPVMEPVGIPSALGTQHNPEALISMRDLWKTYQMGTE
                     AVHALHGVSFDVHKGEYIAIIGPSGSGKSTLMNLIGCLDTPSQGEYWLNGKNVSEMDD
                     DELARIRNQEVGFVFQTFNLLARATALHNVELPLIYGGISSSQRHEMAKKALGAVDLT
                     DRMMHRPNELSGGQRQRVAIARALVNHPSILLADEPTGALDTHTSMEIMDLFERLHSE
                     GNTIIVVTHEHDIAARAHRILSIRDGLIEKDEKIR"
BASE COUNT          636 a          708 c          689 g          482 t
ORIGIN      
        1 atccgtgcaa aattattgaa tttgaactgc attccaacga aaacaatctg tagaaggaca
       61 aatcatggct ttgagtcgta agaagaagat cattattgca gtgtccgctg tggcggtcct
      121 cgctctcgta atcatcatta gtgttttcgc cagccgcaag gaggagtctg aggtcactac
      181 cgttaagatc gaagtcagac ctgagctacg tcagaccgtg accgcctccg gcgaagtgcg
      241 ccccattcgc tacatcaagc tcaccagtga agtgcaaggt cgcatcgaag agatctacgt
      301 aaatgccggc gacatcgtga ctcggggtaa acccctcgtt cgtatcgatc ccacccaact
      361 gcaatcaaat caggaagcac agtgggccgc ggcgcaggcg tcaatgaatg acgtgcaatc
      421 ggcacgcaat gccgtctctt ctgcgcagca gggtttagtg gtagccgagg cggcggtttc
      481 ccaggcgcgc cagcaactag ttgcggtcca gaattccgtc gagcgagcgc aagtggatct
      541 gaataccgcg caacgcgaat tgaaacgcaa cgccgatttg atcgaagccg gagttgcttc
      601 ccgatcagag tatgacgccg ctcgcgatcg ctttgaccaa gcgaagatcg ccctcgacac
      661 cgccagggca aatctggaat cgcaaaagat cgccgtcaag gaagcgatgg aacgcgccaa
      721 ccaacaacgg atcgccgttc gcgacgcacg caccggaatc aaatcttcag aaatgcgtgc
      781 cagccaacaa caggctttgc tgcgtggtca atcgagccaa cgatcaaaag caactcagct
      841 ttcgccgctg aacggcgtga ttgccgatat cccggcgcga gtcggcgagt ttgccgtagc
      901 cggtctctcg accacttcgc tgatgacgat cgccgatatg tcaacgatta acgtagaagt
      961 gaatgtcgac gaaactgaaa tctcaaatgt tgaagtggga cagccagtga aagtgaaagt
     1021 ggacgcgctg ggtgaaaaag agattaccgc cgttgtcaat caaaagaatc cattggcagt
     1081 ttccaagtct gatactcagg gtggactttc cgcgcgtgtg aacgtacagg aggccaagga
     1141 gtttaaagtc actgttgaac taagggattt gaccgacgag gttcgcaacg ccttacgccc
     1201 gggcatgagc gcgactgcca ccatcacaac caaggtaaaa aacaacgtcg tggctgttcc
     1261 gctcgaagca attgtcgaga aatcgccaaa cgcatcgcct tcgccggcgg caaccgttgc
     1321 gggcagcgct cctaatgcgc ctgcgggaga gaagccaaag aatctgaaag gcgtttatgt
     1381 catcgaggga tccaataagg tcaggttcat tgaagtaacc accggaatca caggtgaggc
     1441 cgacatcgag gttacgagcg gcgtgcaagc gggaatggag attgttcgcg gacccagccg
     1501 cgtgctaaag acattgaagg acggaatgtc cgtcaagcgt caggtcaaga aacccggcgc
     1561 caacgctaac gaggccagct aatgagcgac gccaccctga taacccccgt gatggagccg
     1621 gtcgggattc cgtctgcatt gggcacgcaa cacaatccgg aggcgctcat ctcgatgcgc
     1681 gacctttgga agacctacca gatgggaacc gaggcggtgc acgccctgca tggggtgtcg
     1741 tttgacgttc acaaaggtga atacattgcc atcatcggac cttcgggttc cggcaagtcg
     1801 acgttgatga atctgattgg ctgcctcgac acaccctccc agggcgaata ctggttgaac
     1861 ggaaaaaacg tctccgaaat ggacgacgac gaactcgccc gcattcgcaa ccaggaagtg
     1921 ggcttcgtgt ttcaaacctt caacctgctg gcgcgcgcca ccgcacttca caatgtcgag
     1981 ctgcccctaa tatacggcgg catctcctcg tcacagcgac acgagatggc taagaaggca
     2041 ctgggcgccg tcgatctcac ggatcgcatg atgcaccggc ccaacgagct atcgggcggc
     2101 caacggcaac gcgttgcgat tgcgcgcgca cttgtgaacc atccgtcaat ccttcttgct
     2161 gatgaaccta cgggcgcgct cgacacacac accagcatgg agatcatgga tcttttcgaa
     2221 cgtctccatt ccgaaggcaa cacgattatc gtggtcaccc acgaacacga catcgccgcc
     2281 cgcgctcatc gcatcctctc cattcgcgac ggactgatcg aaaaagacga gaagatcaga
     2341 tagggacagc gaggattggg tcagcgtgag ctctaatgaa acgcgatcag cccgatgaga
     2401 tcacttcaaa cgggatgccc aacgcctccc ggcgtagttt ggcgttatcc agccgtcctg
     2461 caaatccggg caaatctcgc ctccgcattt ttcgcgtcac ggctctctgg gaagc
//
LOCUS       DASUEX010000295         2891 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_60507, whole genome shotgun sequence.
ACCESSION   DASUEX010000295 DASUEX010000000
VERSION     DASUEX010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2891
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60507"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..831
                     /locus_tag="VFV61_04950"
     CDS             <1..831
                     /locus_tag="VFV61_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899858.1"
                     /translation="PVTGLVITNGTLKGVSVKSEAGPEEYFADLVIDATGRTRALVRR
                     VNQLSTRTKQHKAPFVAFKAHLRNARAHAGACEIYFYDGGYGGLNSIEGDLSNLCFIV
                     SARVARANNADPERVMRQVVMRNERAATTLKEAQAAQSWMAVSLEQFGRQTLVPVDGL
                     LTAGDAAAFIDPFTGSGILMALESGELIATTILSHLMKACFSFARLADDYRAAHAKVF
                     DSRLRMSALLRRAAFMPRLTAAAIFLSGNDWLRRMLTRATRGGPDGGPACFYRQELSD
                     "
     gene            1120..>2891
                     /locus_tag="VFV61_04955"
     CDS             1120..>2891
                     /locus_tag="VFV61_04955"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEX4899859.1"
                     /translation="MSAFPAQLLKKSIQIGFLFFVTVLLTGVALGQAQSNAADLNGFV
                     RDPQGAVVVGATVTARNPATNFSRSVTTNDEGYYQITSLPPGEYEISVEATNYKKAIV
                     PSYKLTVGARADLDIGLELGQLTEVVNISTEDQPIVETSRTTVANTIEQQRIDNLPIN
                     QRDYLGFSTTISTVGRGNDRPIGPAPSSGLNIGGQRGRSTLVQVDGADNTDNSVNAAR
                     STVSQEAVQEFQVTTNSYAPEFGRATGGIINVVTKGGTNNFQGNIFGFLRNRKFQSRN
                     AFAPVEDPPFTRAQYGATFGGPIVKDKTFFFAAFEQRRRQESGFFTSNVAAGLTSSVT
                     IPAGILPVAQTFTNLTAAQAAFIGGELASGDAARIGRAVAYAHFASIGGQIGLNGTSN
                     LINFLPGVGVAQGSVVGSRFLLAGTPIPTLANNGIVPGFITVNDNGDAVAFRALNQLL
                     RIFPLSEATTFFSVRGDHNFNSNHHLTMRFGYNPSDLTGIQDESQNQTLGQNDFSRTG
                     IQTLRDTSFVASLNSTLSTRAVNEFRFAFGRRKATFDSQIVGGPALQISGTAFIGPNP
                     FSPVDRTEDRFQFADNVNWLVGNHS"
BASE COUNT          661 a          795 c          770 g          665 t
ORIGIN      
        1 ccagtgaccg gcttggtaat tacgaatgga accttgaagg gtgtttcagt caaaagtgag
       61 gctggcccag aagagtattt cgcagattta gtcattgatg cgacgggcag aacgcgtgcc
      121 ctggtccgcc gcgtcaatca gttatctacc agaaccaaac agcacaaagc acctttcgtg
      181 gccttcaaag cgcatctccg taacgcgcgt gctcacgctg gcgcctgcga gatctacttt
      241 tacgacggcg gttacggggg attgaattcg attgagggtg acctgagcaa tctctgtttc
      301 atcgtttcag ccagagtggc gcgggcgaat aatgcggatc ctgaaagagt catgcgccag
      361 gtcgtaatgc gaaacgagcg cgcggcaacc acgttgaaag aggcacaagc agcacaatcc
      421 tggatggcag tttcgcttga acagtttggc aggcagactc ttgtacctgt tgatggcctg
      481 ctcacggctg gtgacgccgc cgcttttatc gatccgttta ccggaagcgg aatcctcatg
      541 gctctggaga gcggggaact tattgctaca acgatcctta gccatctcat gaaggcttgc
      601 ttctcttttg cgcggctggc tgacgattac cgggcagcac atgcgaaggt ctttgattca
      661 cgcctacgga tgtcagcttt gttacgacgg gccgctttca tgccgcggct gaccgcagca
      721 gcgatcttct tatcgggaaa tgattggcta aggagaatgt tgacgcgggc cacgcggggt
      781 gggccagatg gaggaccagc ctgcttctat aggcaggagt taagtgactg atagtgcaac
      841 attttgtaga gaattcaatt tttcaaagcg cttgccacca actgggtact taaggtaaaa
      901 aggctgcgca ttcttcgtga atgttccttg gcaccatcct atgggtgtta atagttgtga
      961 ggcggtagat tcactccccg cgcaagccgc cggcaactaa attaagcagc tctgtttcgt
     1021 agccggcaac aactgacggc acagtccagc aatagcattc aatttagtta acggatgggc
     1081 ctcccagcgg gcccggccaa ctcttaagga gaagatgata tgtctgcgtt ccctgcgcaa
     1141 ctactaaaga agtctatcca gatcggcttt ctgtttttcg tgaccgtcct gttaacgggc
     1201 gtcgcgcttg gccaggcgca atcaaacgcg gccgacctaa atggatttgt gcgtgatccc
     1261 cagggagctg tggtcgtagg tgccaccgtg actgcgcgca acccggccac aaatttctcg
     1321 cgcagtgtca caacgaatga tgagggttat taccagatca ccagccttcc cccaggcgaa
     1381 tatgaaatat cagtggaagc gacaaactat aagaaggcga ttgttccttc ttacaaactg
     1441 accgtcggtg cccgcgctga tttggacatc ggtctggaat tgggccaact gacagaggta
     1501 gtgaacatct cgactgaaga ccagcccatc gttgaaacca gccgcaccac cgtcgccaat
     1561 acgatcgaac agcagcgcat tgacaacctg cccatcaatc agcgggacta ccttgggttc
     1621 tcaaccacca tctcaactgt cggtcgcggt aatgatcgac ctatcggccc ggcaccctca
     1681 tcgggactga acattggtgg acagcgtgga agatccaccc tcgtacaagt ggacggcgcc
     1741 gacaacactg acaactcagt caatgcagcc cgcagcacgg tttcacagga agcagtgcag
     1801 gaatttcagg tcaccacgaa ttcctacgcc ccggaatttg ggcgcgcaac gggcggcatc
     1861 atcaacgtcg ttacgaaagg tggaacaaac aattttcagg gaaatatttt tggcttcctg
     1921 cgtaaccgga agtttcagtc gcgaaacgcc tttgcaccag tcgaggatcc gccgtttact
     1981 cgggctcaat atggcgccac gtttggcggt ccgatcgtga aagacaagac cttcttcttt
     2041 gccgcattcg agcagaggcg gcggcaggaa tcggggttct tcaccagtaa cgtcgccgca
     2101 ggtctaacct ccagcgtaac tattccggcg ggaatcctgc ccgttgcaca aacattcact
     2161 aatctgacgg cggcccaggc ggcttttatc ggcggtgaac tggccagcgg agacgcggct
     2221 cgaattggtc gcgcagttgc ctatgctcac tttgcatcga ttggcggtca aatcggactc
     2281 aacggcacca gcaatctcat taacttctta ccaggcgttg gcgtcgcgca gggcagcgtg
     2341 gttggctcgc gattcctgct ggctggcacg cccataccga ccctcgctaa taacgggatt
     2401 gttccgggtt tcatcactgt aaatgataac ggtgatgcgg tggcattccg cgcgctaaac
     2461 cagttgctaa gaatctttcc gctttccgag gcgacaactt tcttctccgt tcgcggcgat
     2521 cacaacttta attcgaacca ccacctgacg atgcgctttg gctataaccc aagcgatctc
     2581 accggcatcc aggacgagtc gcaaaaccag acgcttggtc agaacgattt ttcgcgcacc
     2641 ggcattcaaa ccttgcgcga tacttcattt gtcgcgagcc ttaactcaac gctttccacc
     2701 agggccgtga acgagtttcg ctttgctttc ggcaggcgaa aagcaacgtt cgattcacag
     2761 attgtcggtg gacctgccct gcagatttca ggtacggctt tcatcggtcc taatccattc
     2821 tcgcctgtcg atcgcactga ggaccgtttc cagtttgccg ataatgtcaa ctggctcgtc
     2881 gggaaccact c
//
LOCUS       DASUEX010000296         3776 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_158174, whole genome shotgun sequence.
ACCESSION   DASUEX010000296 DASUEX010000000
VERSION     DASUEX010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3776
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158174"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1620
                     /locus_tag="VFV61_04960"
     CDS             <1..1620
                     /locus_tag="VFV61_04960"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX4899860.1"
                     /translation="QSTQRNRYLGFFAHDEWRAVDNLTVSFGLRYERESIIDDGNNWA
                     PRLSLAYDPFRSGRTVLRAGAGIFYNRALLRTVDDFTLGATQRFFDTNALRDPISGRL
                     LTAAQRREFIAANLSFPHTLTADSPLVTHYGVLNTGFSRRLDPSLRIPESYQANLGVE
                     RDLGGGFTFEANYTFNRGLHLWRELNINAPRLPAGFRNLSQYLASQDFVNFRNTSTGL
                     RPLYNATTAGELIRFVFSSSDPANPNAIGQMFEFGVPVSLINLNSVSSSTAIDVALAA
                     VQGLRPDPSRTEVEQLISAGNSYYRGLTFEIRRRFNASKNFDFAFRAAYTHSHLIDDG
                     VVNTSDALTPGDFQHERVRSLLDRRHRFVVSGTFTMPRQLGALRLAPIFRAASGAPFN
                     ISSGGVDRNLDDVNNDRPNFTGDANTLRWRGPGSAIDAAILNYFSPATIGQAGTLPRN
                     AGRGPGLILFDLSITREFRINERVRLRPVVEFDNVLNKTVFSFGAEFINFNALSSSAS
                     AAQRQAFLDSFLLTTRTLRQRQIRVGLRFDF"
     gene            1835..2572
                     /locus_tag="VFV61_04965"
     CDS             1835..2572
                     /locus_tag="VFV61_04965"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HEX4899861.1"
                     /translation="MADTSLNSDSQAKTGPAAEVDSALLDAELFVKYKAPERAIKRIK
                     TALERNPRSIPLRERLREIAAAHKHPDEAARQGLALASLYIERDDFDSAYDRLLEAKQ
                     LDPRINVAKGLEAIRKARRPDLPQSPVAQAERELKREYVTLAGDLAAVSLFDAIQVVE
                     NAKLTGALILSSEAQTGRVLFNDGRIVDAEAAGARGSLAFRYIVEITNGSFEFQKSPQ
                     LFPVVLQAVSNTNLILDTLQQLDEAKA"
     gene            complement(2745..3506)
                     /locus_tag="VFV61_04970"
     CDS             complement(2745..3506)
                     /locus_tag="VFV61_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899862.1"
                     /translation="MSLEFAVAYPCVLRARHSEKQLRDWGRLSNLHTQLTTGDTSAPS
                     EDKLHWVENASTEVETMQIETEVCPHCPSFLPIEAAGEGESVGCLGRITYPIEAQFEK
                     FIADRVQLCLDTLDKEDQPRLLRILVDAESPFDGEATKELRRVTTREGLRFYELRLPI
                     RLTREAVALSTDNVFDLLAGFRSEDYEQTSYTRELPAAATADYYDFLDLLLRNDLNQS
                     EVARLTTRSKNYSQFLRLLAAIERAEALNTRLLID"
     gene            complement(3557..>3776)
                     /locus_tag="VFV61_04975"
     CDS             complement(3557..>3776)
                     /locus_tag="VFV61_04975"
                     /inference="COORDINATES: protein motif:HMM:NF040124.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6526 family protein"
                     /protein_id="HEX4899863.1"
                     /translation="IRLEERIRYQQLLPPSLVQQASVLSPSQIVALRFAADDELAELL
                     GAVVSGKLSDPKAIKRAITNWRSDTFRV"
BASE COUNT          835 a         1054 c         1039 g          848 t
ORIGIN      
        1 cagtcgacgc aacgcaatcg atacctcggt ttctttgccc acgatgagtg gcgtgcggtc
       61 gacaatctca ccgtgagttt tgggctgcgt tatgaacgcg aaagcatcat cgatgatggg
      121 aacaactggg cgccacgtct ctcgcttgct tacgatccat ttcgctccgg aaggacagta
      181 ctgcgcgccg gcgcgggaat tttctacaat cgcgccttac tgcggactgt cgatgacttc
      241 acgctcggtg ccacgcagag gtttttcgac accaacgctt tgcgcgaccc aatcagcggc
      301 aggttgctta ccgcggcgca aagacgcgag ttcattgccg cgaatcttag ttttccccac
      361 acactcacgg cggattcgcc gctcgttacg cactatggag tgttgaatac gggattctcg
      421 cgacggctag atccttccct gcggattccc gaaagttatc aggcgaatct gggagtggaa
      481 cgcgatctgg gaggcggttt cacattcgaa gcgaactaca ctttcaatcg cggacttcat
      541 ctttggcgag aactgaatat caacgcaccg cggctgccgg ctggttttcg caacctttct
      601 caatacctgg cttcccaaga tttcgtcaat ttccggaaca cgtcgacggg cttgcgtccc
      661 ttgtacaatg cgacaacagc gggcgagttg attagatttg tcttcagtag ttccgatccg
      721 gcgaatccca acgccatcgg acaaatgttc gagtttggtg ttcccgtttc gctgataaat
      781 ctgaacagcg tttcgtcgtc aaccgcgatt gatgtcgcgc tggcagcggt gcaaggtttg
      841 cgccctgatc cctcgcgcac cgaggtagag cagttaattt ctgccgggaa cagttactac
      901 cgcggcctga cttttgaaat ccgcaggcgc ttcaatgcga gcaagaattt cgattttgct
      961 tttcgcgctg cttacaccca ctcacacttg attgacgacg gagtcgtcaa tacgtctgat
     1021 gcactcacgc ccggtgattt ccagcatgag cgtgtgcgca gcctgcttga tcgccggcat
     1081 cgctttgtag tctctggaac ttttaccatg ccacgccaat tgggagcgct tcgcctggcg
     1141 ccaatctttc gtgccgcctc gggggcgccc ttcaacatct ccagcggcgg ggtcgatcgc
     1201 aatctggatg atgtcaacaa cgatcggccc aacttcacgg gggacgcaaa cactctgcgt
     1261 tggcgtggtc ccggctctgc aattgatgct gccattctga attatttctc gccagcaacg
     1321 atcggccagg caggaacact accgcgcaac gcgggacggg gaccgggttt aatcctgttc
     1381 gaccttagta ttacgcgtga gtttcggatt aacgaacggg tgcgtctcag accggtggta
     1441 gagtttgata acgtcctcaa caagacggtg tttagtttcg gcgccgagtt tatcaacttc
     1501 aacgccctgt catcgagcgc cagcgccgcg cagcggcagg cattcctgga ttcatttctt
     1561 ttgactacac ggacgctgcg gcagcgacag attcgagtag gcctgcggtt cgatttttag
     1621 agctgcgcat ttcgcggcta ttcggatgat ttaggggatc gccattcgcg ttcttgccga
     1681 aaaagctttc cattaataaa cactaatctg cgcgaaagaa actcggggcg ggcagtgcca
     1741 tccaggcaac acgaaggacg agaagcggag gcccgcccta tgctaaactc gccgccggtc
     1801 gcattttcgt ccccgttgag aacgaaaact ccggatggct gacacttccc tcaactccga
     1861 ttcgcaagct aaaaccggcc ccgccgccga ggtcgactcg gcgttactcg acgcggagtt
     1921 gttcgtcaaa tacaaggctc ctgagcgcgc catcaaacga ataaagaccg ccctcgaacg
     1981 aaacccgcgc tcgatacctt tgcgcgaacg cctgcgagag attgctgccg cacacaaaca
     2041 tccggacgaa gccgcccggc aaggcctggc gcttgccagc ctctacatcg aacgcgacga
     2101 ttttgatagc gcctacgatc gcttgctgga agcgaaacaa ctggatccgc gcatcaatgt
     2161 ggcgaagggc ttggaagcga ttcggaaggc gcgccggccg gatctgccac agtctccagt
     2221 tgctcaagct gagcgagagt taaaacgcga atacgtaacg cttgcaggtg atctcgcagc
     2281 agttagtctg tttgatgcga ttcaggtagt ggaaaacgcg aagctcaccg gcgccttgat
     2341 tctttcaagt gaggcgcaga cgggccgggt gcttttcaac gacggcagga ttgtggatgc
     2401 cgaggctgct ggggcgcgcg gtagcctggc ttttcgctac atagtggaga taacaaacgg
     2461 cagctttgag tttcaaaaat ccccgcaact attcccggtt gtgctccagg ccgtaagtaa
     2521 taccaacctg atcctcgata ctttgcaaca actcgacgag gccaaggctt agccgaaggt
     2581 cgttccgcac acattcaacc gccaagctgc cacgaaacgc caaggcgcca ggcaataaat
     2641 cgttcgcgaa tcctccagct taggcaaaaa gtattctcgg cgaactctgc gcttagcggt
     2701 ggggtttttg agtggctacc gcggcttctg attgagtctg aatctcaatc gatcaagagc
     2761 cgcgtgttta acgcctcagc ccgttcaata gcagcgagca agcgcaggaa ctgggagtaa
     2821 ttcttgctgc gagtggtgag gcgcgctact tcggattgat tcaggtcgtt gcgcaggagc
     2881 aaatcgagga agtcgtaata gtcggctgtc gccgctgccg gcaactcccg tgtatagctg
     2941 gtctgttcgt agtcttcaga acgaaaacca gcgagcagat cgaaaacatt gtcggtagaa
     3001 agcgcaaccg cttcacgcgt caggcggatc ggaagccgaa gttcgtagaa acgcaagccc
     3061 tcacgtgtag taacgcggcg caactccttc gtcgcttcgc cgtcaaaggg agactcggca
     3121 tccacgagaa tgcgcagcag ccgcggctga tcctccttat caagcgtgtc aagacaaagt
     3181 tggacgcggt cggcaatgaa tttctcaaac tgcgcttcga tgggataggt aatgcggccg
     3241 agacagccaa ccgattcacc ctcacccgcg gcctcgatcg gaagaaaaga ggggcagtgc
     3301 ggacaaactt ccgtctcgat ttgcattgtc tcgacttccg tactcgcatt ttccacccag
     3361 tgaagtttgt cttccgaagg cgcactggta tcgccagtgg tcagttgtgt gtgcaggtta
     3421 ctcaaccggc cccaatcgcg tagttgtttt tcggagtgac gcgcccgtag gacgcaagga
     3481 taggcgacgg caaattccaa cgacatgctc taacagtaaa ctacctgggt gcgaaagcga
     3541 aaatcggccg atcgaatcaa actcggaagg tgtcgctgcg ccagttcgta atggctcgct
     3601 tgatcgcttt gggatctgaa agctttcctg acacgaccgc tcccagaagc tcggctaatt
     3661 catcatctgc ggcgaatcgc agcgcgacga tttgactcgg gcttaaaacg ctcgcttgct
     3721 gcacaaggga gggtggcagc agttgttggt aacgaattcg ttcttcaagt cggatg
//
LOCUS       DASUEX010000297         3800 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1092554, whole genome shotgun sequence.
ACCESSION   DASUEX010000297 DASUEX010000000
VERSION     DASUEX010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3800
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1092554"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3389
                     /locus_tag="VFV61_04980"
     CDS             <1..3389
                     /locus_tag="VFV61_04980"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX4899864.1"
                     /translation="IHAIEEADGLDFIVMEMVEGTTLKSLIYGEGALPLMRLLDLGIQ
                     TAEALEAAHEINIIHRDIKSSNILVTSRGQAKVLDFGLAKTVRTIASSVDHDAPTLTN
                     LTDEGTVLGTAAYMSPEQTRGQVLDRRSDIFSLGCVLYEAATRTLPFTGPSMLAVMHA
                     IATHAPSPPSLLRPDLPKELDLILERAMAKDKEKRYSAAAEMAHALRSLRSTVTGEWL
                     GGPIIFDKDLAGSSSTPFVGRGPELSKLEGILQQAIDGSGRIVFITGEPGIGKTSLGD
                     EFLKRARARFPGLSVSRGRCVEQYGTGEAYLPFLDAVGSLLEGPARDRLAAIMRTSAP
                     TWCWQLPTAFTSTGAIETLQQETIGATKERMMREMGDALALFATTSPVVILLEDLHWA
                     DPSSVDLLRHLCQRISNQRLLITGTFRPEDIERSNHSLKGLKAEMQTHQLCEEIALDS
                     FTVSHIQDYLNDLFAPHGFPPEFAERIHDKTEGHPLFAMNLLQYLQERGDIAKTNEHW
                     SLSRPLAEIELEVPQSVRSMISKKVDALGENERRTLEYASVEGQEFLSTVAASLLGVD
                     EVDLEELLARIEKNHRMIVSRGEEELPDGSLATRYRFAHALYQNFLYDGLVTKRKTML
                     HKQAGETLLELYGKRAPHLAAPLALHFERGREFPRAVEFLIHAGDNATALYANAEAAE
                     HYTRALSLAEKLPAEGQAETKATLYAKRGATNMALSRFDESIDDYRRMLKHQQVFDSP
                     EKQAGGLIALATTLFFAHRLEEMELRVNEALDAAKRAGSEKLRLDTMGLMALKHAAYG
                     ELAQGRPILDDVIKSSRAIDHKQGLLTGLTWRGCIHFFQTEYELAIECELEARKLASK
                     LRDGFLLLTSMFFLGLSQGNLGRISDAIATLEEAIQMAGRNGDGFWFPRMPNCIGWMH
                     RELQDFEGALKYDQEGLRIARQYHVLEAEANSLINLGIDHTIGGRPDETAAAFSETRN
                     IFEHDAWFRWRYNIRLEAATGWHWLRQGNVDKAEEFSRRLLDTANEHQVHKYIAEAHR
                     LQAQIAIVRGDFDTAANEFAAGLEELESYPAPLVAWRTYADLGHLHRKRGEATSAQSA
                     LRRAAEIIDACAASVSDEVLRATFLNSQIVREVKAGAGKE"
     gene            3433..>3800
                     /locus_tag="VFV61_04985"
     CDS             3433..>3800
                     /locus_tag="VFV61_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899865.1"
                     /translation="MANSLRAGNWVSIGACLMFGIAVALSGCGSNKSTTNSEPSESRA
                     AKSDVAQPEAGVEKAKPAAGTGNVQGKVLYNSQPAANIEIKLCETFNRFMGGCTGKSY
                     TVRSDKDGEYLITNVAPKTY"
BASE COUNT          889 a         1000 c         1087 g          824 t
ORIGIN      
        1 cgatccacgc cattgaagag gccgacggcc ttgatttcat tgtcatggag atggtcgaag
       61 gcacgacctt aaagagcctg atttacggcg agggcgcact gccactcatg cgtctcctcg
      121 atctcgggat ccagacggca gaagcgctgg aagcagcgca cgaaatcaac atcatccatc
      181 gcgatataaa gtcgtcgaat atcctggtca cttcccgcgg acaggctaag gtgcttgatt
      241 tcggccttgc gaaaacagtt cgcactattg ccagcagcgt tgatcacgat gcgcccactc
      301 tcacaaacct gactgacgaa ggaacggtgt tgggcactgc ggcctacatg tcacctgagc
      361 aaacgcgagg acaagttctc gatcggcgtt cagatatttt ctcgctgggt tgtgtcctct
      421 acgaagccgc gacgcgcacg ttacccttta ccggacccag catgcttgcc gttatgcatg
      481 ccatcgccac tcacgcgcca tcgcctccga gcctgctgcg accggatcta ccgaaggaac
      541 tcgatctgat cctcgaacgg gcaatggcca aagacaagga gaagcgttat tccgcagcgg
      601 ccgagatggc tcatgctcta aggagtttgc gcagcaccgt caccggagaa tggcttggtg
      661 gtcccatcat ctttgacaag gatctagccg ggtcttcctc gactccgttc gttggccgag
      721 gtccagagtt gagcaagctt gaaggaatcc tgcaacaagc tattgatggt agcgggcgaa
      781 tagtgttcat cacgggcgag ccgggcatcg gcaaaacctc gcttggtgac gaattcctga
      841 agcgtgctcg tgctcgtttt cctggtttgt cagtttctcg cggacgctgc gtcgaacagt
      901 acggcactgg agaggcttat cttccgtttc tcgatgcggt cggctcattg ctggaaggac
      961 cagcccgcga tcgactggcg gcgattatgc gtacctcagc accaacctgg tgttggcagt
     1021 taccaaccgc tttcacatca accggcgcta tcgaaacact gcagcaggag acgattggcg
     1081 cgaccaaaga acgtatgatg cgggagatgg gagacgcgct ggctctcttt gccacgactt
     1141 cgccggtcgt gattctgctg gaagacctgc actgggcaga tccttccagc gtggatctgc
     1201 tgcggcattt gtgccagcgc atcagcaatc agcgactgtt gattacgggg acgttcagac
     1261 ctgaagacat tgagcgaagt aatcattcac tgaaaggtct caaggctgaa atgcagaccc
     1321 accagttatg tgaggaaatt gcgctcgatt cattcactgt ctcccacatc caggattacc
     1381 tcaacgacct gttcgcgccc catgggtttc cgcctgagtt tgccgagcgt attcacgaca
     1441 agaccgaagg tcatcccctt tttgccatga atctcttgca gtacttgcag gaacgtgggg
     1501 acatcgccaa gacaaatgag cattggtcat tgagccgccc gctggcagaa attgaactgg
     1561 aagttccgca gagcgtgcgc agcatgatca gcaagaaagt cgacgcgctc ggagagaatg
     1621 aacgccgtac tctggagtat gcgagtgttg aaggacagga atttctttct accgttgccg
     1681 ctagccttct tggtgttgac gaggtcgatc ttgaggagct gctcgcgcgc atagaaaaga
     1741 atcatcgaat gatcgtgagc cgcggcgaag aagaattacc cgatggctcc ctcgctacac
     1801 gttaccgttt tgcgcacgcg ctctatcaga attttcttta cgacggcctg gtgaccaagc
     1861 ggaaaaccat gctgcacaag caggccgggg aaacattgct ggaactctac ggcaagcggg
     1921 cgccacatct tgcggcaccg ctggcgctgc acttcgaacg tggacgcgag tttccccgcg
     1981 ccgtcgagtt cttgattcat gctggcgaca acgccaccgc gctttatgcg aatgctgaag
     2041 ctgcggaaca ctacacgcgt gctctgagtt tggcggagaa gttgcccgca gaagggcagg
     2101 cagaaaccaa agcgaccctg tatgcgaagc gcggcgccac taatatggcg ttgagccgtt
     2161 ttgacgaatc gattgacgac tatcgcagga tgctcaagca ccagcaggtc ttcgactcgc
     2221 cagaaaagca ggcaggtggg ctcattgctt tggctacgac cctgtttttt gcccatcgtc
     2281 ttgaggagat ggagttgcgg gtcaacgaag ccctcgacgc cgctaaacgg gctggcagcg
     2341 agaagttgcg tttggacaca atggggctga tggcgctgaa acacgccgcc tacggagagc
     2401 tggcccaagg tcgtcctatt ctggatgatg tgatcaagag ttcgcgcgcc atcgatcata
     2461 aacaggggct gttaacaggg ttgacctggc gcggctgtat ccatttcttc cagacggagt
     2521 atgagctggc gatcgagtgt gagctggagg cgagaaagct tgcctcgaag ttgcgtgatg
     2581 gatttctgct tttgacctcg atgttctttc tcggactatc acaaggaaat ctcggaagaa
     2641 tctcagacgc gattgccact ctcgaagaag caattcagat ggccgggcgc aatggtgacg
     2701 gtttctggtt tccgcgaatg ccaaattgta tcggctggat gcatcgcgag cttcaggact
     2761 ttgagggggc actaaaatat gaccaagagg gattaaggat cgcacgtcag tatcatgtcc
     2821 tcgaagccga agctaattct ttaattaacc tgggcatcga ccatacgata ggcggccggc
     2881 ctgatgaaac tgcggccgca ttcagtgaaa ccagaaacat cttcgagcac gatgcctggt
     2941 tccgttggcg ctacaatatt cgcctggaag ccgcaaccgg ctggcattgg ctgcgacaag
     3001 gcaacgtcga taaggctgaa gagttttctc gccgcctgct cgacaccgca aacgagcatc
     3061 aggttcataa gtacatagcg gaagctcaca gacttcaggc gcagatcgcg atcgtccgcg
     3121 gtgattttga taccgctgcg aatgaattcg ccgccggcct tgaagagttg gaaagttatc
     3181 ccgcacctct ggtcgcgtgg agaacgtatg ccgacctggg ccacctgcat cggaagcgtg
     3241 gggaagcaac atcagcgcag tcggctctca gacgcgcggc agaaattatt gacgcgtgcg
     3301 ccgcgagcgt cagcgatgag gttttgcggg caaccttcct taattctcag atagtacgcg
     3361 aggtgaaggc tggcgctggg aaagaataat tgtcaacgaa cagtctcaac aaaaaggtga
     3421 aaggttctca caatggcaaa ttcgttaaga gcaggtaatt gggtttcaat cggcgcttgc
     3481 ctgatgtttg ggatcgccgt tgcgctcagc ggttgtggca gcaataaatc aacgacgaat
     3541 tctgagcctt cagaatccag agcagcaaag tctgacgtgg cacagcctga ggctggggtc
     3601 gaaaaagcga agcccgccgc gggcaccggg aatgttcagg gcaaggttct ctataacagc
     3661 cagccagctg cgaacatcga aattaagttg tgcgagacgt tcaatcgctt catgggaggt
     3721 tgtacgggaa agtcttacac cgtgcgcagc gacaaggatg gcgagtattt gattaccaac
     3781 gtcgcaccaa agacgtacga
//
LOCUS       DASUEX010000298         3158 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1299203, whole genome shotgun sequence.
ACCESSION   DASUEX010000298 DASUEX010000000
VERSION     DASUEX010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3158
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1299203"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1978)
                     /locus_tag="VFV61_04990"
     CDS             complement(<1..1978)
                     /locus_tag="VFV61_04990"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEX4899866.1"
                     /translation="MSAMLARLSAETKIRPDASIRRALFVAAFIVFWVFAISVRLVYL
                     QVSQHEALGERARSQQQQELETSSQRGLLLDRQERELARSIQTESIFIDAAKLENAGD
                     IECTAARLAPVLQVKKEDLQRKIADAKTKNSRFVWIARRLSAEQATAIRGLGLPGVDF
                     RSEPKRFYPNGSIAAHVLGFVGVDGAGLGGVEQFYNEKITGEPGKLFLERDSEGNPYE
                     SFEQPGTPGQTIVLTLDHSIQYRAEQALLSAVTKSRAKSGTAIVLDPRSGEILALANA
                     PTFDPNKPGEAKAEARSNWALQNIYEPGSTFKIVSFSAAIEKGLAKPDDRIDCQMGAI
                     TVAGRLVHDHHAFGSLTITEALAKSSNVAAIKLGMRVGDATMYEYMKRFGFGSRTGIE
                     LPGETAGLLRPVSRWQPSSIGSIAIGQEVGVTPLQMAAAFGALANDGVRIAPHLVREV
                     RTASGATVYRANPEQRRVVNVETARALRQMLESVTLNGTAKLAQLNGYSAAGKTGTAQ
                     KIDPKTKTYSRTKHVASFVGFAPVNDPALVIIVVIDEPGGAYHGGDVAAPVFRDIAEQ
                     ILPDLGVVPDTYFKLPDGLVAEAATPEVAPRSREAEVQTELAREATLPSVAGRDGHGG
                     EIVYAAANKSSILMPDLSGRSVRDVARTCARLGLK"
     gene            complement(2054..2443)
                     /locus_tag="VFV61_04995"
     CDS             complement(2054..2443)
                     /locus_tag="VFV61_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899867.1"
                     /translation="MNRHPLKQQNSNVHRERDLRALSRLALQLGCALALCGGFVFAAR
                     QHFAAVHYGYESETLRVERQKLLEEQQRLILERERASSPAHLELAARQLGLKPLQPGQ
                     VGTRKPETGRQLPVATALLHQSASFNR"
     gene            complement(2461..>3158)
                     /gene="rsmH"
                     /locus_tag="VFV61_05000"
     CDS             complement(2461..>3158)
                     /gene="rsmH"
                     /locus_tag="VFV61_05000"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:TIGR00006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEX4899868.1"
                     /translation="EPDAQGVLVDLGVSSLQFDSPERGFSFRWEAPLDMRMNPAAGEE
                     TAADLLARLPEEEIARVLFEYGEERRSRRIAKWIVEKREQGVPIATTRALADLVQRAT
                     GHSKGKGIHPATRTFQALRIAVNRELEGLDHYVGTAIDLLQPDGRFVAISFHSLEDRI
                     IKQELRRLSGYCQCPSRVPVCSCGARKVVEILTKRPVVPDDKEVEDNPRARSAKLRAC
                     RKLSLPTLVDFQL"
BASE COUNT          665 a          934 c          851 g          708 t
ORIGIN      
        1 gcttcaaacc aagtcgcgca caggttcgtg ctacatcacg aacactgcgg ccactcaaat
       61 caggcatcaa gattgaactc ttgttcgcgg ccgcataaac aatctcgcct ccgtgtccgt
      121 cgcgcccggc cacacttggc aaggtcgctt cccgggcgag ctcggtttga acttctgctt
      181 cacgacttcg cggcgcaact tcaggcgtcg ccgcttccgc aaccagtccg tccggcaatt
      241 tgaaatacgt atccgggacc actccgagat caggcaatat ttgttctgca atgtcgcgaa
      301 acacgggggc ggccacatct ccaccgtgat aagccccgcc cggctcgtcg ataaccacga
      361 tgatcacgag cgcgggatcg ttgacgggcg caaaaccaac gaacgaggcc acgtgcttgg
      421 tccgtgaata ggttttcgtt tttggatcta ttttctgcgc ggtgcctgtc tttcctgcgg
      481 cgctatagcc atttagctgg gcgagcttag cagtgccgtt caaggtcacg ctctcgagca
      541 tttggcgcag ggcgcgcgct gtctcgacat tcacaacgcg acgctgttcg ggattggcgc
      601 gataaaccgt tgccccggac gccgtgcgaa cttcgcgtac gaggtgcggg gcgatacgca
      661 caccgtcgtt tgcgagcgcg ccaaaagccg cagccatttg caacggcgtc actcccactt
      721 cctgcccgat ggcgatcgat ccaatagacg atggttgcca gcgactcaca ggtcgcaata
      781 gtcccgctgt ttcccccggc aattcgatgc ctgtgcggga gccgaagcca aaccgcttca
      841 tgtactcgta catcgtcgca tccccaacgc gcattcccag tttgatcgcg gccacattac
      901 tggacttggc caacgcttcc gtaatcgtca ggctgccaaa cgcatgatgg tcgtgaacca
      961 accggccggc gaccgtgatc gcgcccattt ggcaatcaat gcggtcgtca ggtttcgcca
     1021 atcctttttc aatagctgcc gaaaaggaaa caattttgaa agtcgacccg ggctcataga
     1081 tgttttgtag cgcccagttg gaacgggctt cagccttcgc ttcgcccggc ttgtttggat
     1141 cgaaggttgg cgcgtttgcg agagcgagga tctcgccact gcgcggatca agcacgattg
     1201 cagttccgga cttggctctt gatttagtca ctgctgagag cagtgcttgt tccgcccgat
     1261 actgaattga atggtcgagc gtcagaacaa tcgtttggcc gggcgtaccc ggctgctcaa
     1321 aactctcgta agggttacct tcagaatctc tctcgagaaa cagttttccc ggctcacccg
     1381 taatcttctc gttatagaac tgctcgacac cacccaaccc agcgccgtca acaccgacaa
     1441 aacccagaac gtgtgcagca atcgagccat tgggataaaa ccgcttcggc tcggaacgga
     1501 aatccacccc cggtagtccc aggccgcgga tcgcggtcgc ttgctcagcc gatagtctgc
     1561 gggcaatcca gacaaacctg ctgttttttg ttttcgcgtc tgctattttg cgttgcagat
     1621 cttctttttt gacttgtagc actggtgcca agcgcgccgc agtgcattcg atgtcgcccg
     1681 cgttctccag cttcgcagcg tcaataaaga tcgattcggt ttgaatgctg cgggcgagct
     1741 cgcgttcctg tcggtcaagc aagaggccgc gttgcgaact ggtttcaagc tcttgctgtt
     1801 gttgggagcg cgcgcgctca ccgagagcct cgtgctgcga cacttgcagg taaaccagtc
     1861 ggacactgat ggcgaacacc caaaagacaa taaacgccgc gacaaagagc gcgcggcgaa
     1921 tcgaggcatc aggtcgaatt tttgtttctg cggaaaggcg tgccagcatt gcagacatga
     1981 tccccttaaa cggtaccgcg agggaaacag caccaacctt gttcgaacgc gccgaagtgg
     2041 cgaccttact taactagcga ttaaaagaag cagactgatg aagtaacgcc gtagcgactg
     2101 gcagttgtct tcccgtttcc ggtttgcgcg tgccaacctg tccgggttgc aatggtttca
     2161 atcctaattg acgagcagcc aactccaggt gcgcaggtga cgacgcgcgt tctcgttcga
     2221 gaatcagccg ctgttgctcc tcaagcagtt tttgccgctc gacacggagc gtttcactct
     2281 cgtagccgta gtgcacagcc gcaaagtgtt gccgcgcggc aaagacaaag cctccgcaaa
     2341 gcgccagggc acaaccaagc tgcaacgcca ggcgcgagag tgctcgtaga tctcgctcac
     2401 gatgaacatt tgaattctgc tgtttgagcg ggtgtcggtt catggggcta cggtctccat
     2461 tcacaattga aaatcaacca gggtcgggag actgagtttc cggcaagctc gcagtttggc
     2521 acttcgcgcg cgcggattgt cttccacctc tttgtcatca ggaaccaccg gacgcttcgt
     2581 caatatctcg accaccttgc gtgcgccgca ggaacaaacg ggaacacgcg acgggcactg
     2641 gcagtagcct gagagccgcc ttagttcctg cttgatgatc cgatcttcaa gcgaatggaa
     2701 acttatcgcg acgaatctgc cgtccggttg taaaagatcg attgcggtgc cgacgtaatg
     2761 atccagccct tcaagttcgc gattgacggc gatgcgcaaa gcctgaaaag tacgcgtcgc
     2821 cggatggatt ccttttcctt tgctgtggcc ggtggcccgc tggacgaggt cggccaaagc
     2881 cctggttgtt gcaatgggta ccccttgttc gcgcttttca actatccact tggcaatgcg
     2941 acgagagcgc ctctcttcac cgtattcaaa aagcaccctg gcaatttctt cttccggcaa
     3001 tcgcgccagc aaatcggctg ctgtttcttc gccagcggcc gggttcatgc gcatatccag
     3061 cggcgcctcc caacgaaaac tgaaccctcg ttccggtgag tcgaattgca gtgaagaaac
     3121 gccgaggtct actaagacgc cttgggcatc aggttcac
//
LOCUS       DASUEX010000299         4580 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_978695, whole genome shotgun sequence.
ACCESSION   DASUEX010000299 DASUEX010000000
VERSION     DASUEX010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4580
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_978695"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2107
                     /locus_tag="VFV61_05005"
     CDS             <1..2107
                     /locus_tag="VFV61_05005"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEX4899869.1"
                     /translation="LSRMTLEEKLGQLQQLDGEANGNFRPEHRDLVKKGLLGSTLNVR
                     GVTRTNELQRIAIEGSRLKIPLLFGFDVIHGYRTIFPIPLGEAATWDPVAVERSAAIA
                     AKEARASGVHWTFAPMVDIARDARWGRIAEGSGEDPFLGSVMARARVLGFQGSDYSAP
                     DKVVACAKHWVAYGAAEGGRDYNTTDLSERTLREVYFPPFKAALDAGVGTFMSAFNDL
                     NGVPTSANSFTLTQVLRREWKFDGIVVSDYESVKELINHGLAANEKEAAAAGLNAGVD
                     MEMVSRLYNQHGADLLKERKLSMATIDEAVRRILRIKFRLGLFEKPYADEVRERNSVL
                     TKEHLSAARESAARSLVLLKNEGNVLPLSKTLKAIAVVGPLADSQKDVIGSWTGDGKP
                     EDAVTLLAGIKSKLSPQTIVTYARGCEINDDSTSGFAEAVRIARESDVVIVAVGESAE
                     MSGEAASRSSLDLPGRQLDLVRALQATGKPVVVVLMNGRPLSINWIAENVPAILETWF
                     AGTQAGNAIADVIFGDVNPGGKLPVTFPRSVGQVPLYYNHKNTGRPPDANNKYTSKYL
                     DQPWTPLFPFGYGLSYTRFKFSNLQLSARRIRPDGSLTVTVELENVGQRAGDEVAQLY
                     IRDAAASVTRPVKELKGFQRITLQPGEKKRVEFKLTSRELGFYDREMRFLVEPGAVKV
                     MVGSSSEDLITAIFEIEKP"
     gene            2138..2359
                     /locus_tag="VFV61_05010"
     CDS             2138..2359
                     /locus_tag="VFV61_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899870.1"
                     /translation="MKPQKLTVGLFLFAGLLFLIAGLRDIFAPGFFNVSPQVKSRGDI
                     IFEFAAAVTFLTVGYAISLNKRVDSNKKQ"
     gene            2389..3792
                     /locus_tag="VFV61_05015"
     CDS             2389..3792
                     /locus_tag="VFV61_05015"
                     /inference="COORDINATES: protein motif:HMM:NF021596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HEX4899871.1"
                     /translation="MSSARVRPSGLLSIFVIFSLLFSCSPTFARQPISKAVSLHLQGA
                     PVFRLSRQDEVFSEDLQRRSFQYFWEQADPQTGLVPDRARMDRAPLAESHRNVASIAA
                     TGFGLTALCIAAEREWITSQQAAERTRNTLRFFAQKAFQERGWFYHWLDAKTGERKWN
                     SEVSSIDTALLLAGVLTARQYFRADPEIVNLANRIYERVDFKWMLNGDPFLLSHGWKP
                     ESGFLKPRWDTYSEDTILYLLAIGSTTHAIPPASWYAFWRDRYRYEGHSYFTTIGVPI
                     FMHQYSHAWIDFRNRREVKGDRIDYFENSISATLAHRAFCINLAGEFREFGPNVWGIT
                     ASDSAKGYLAWGGPPRDPAIDGTVVPSAAGGSLMLTPDISVAALRTMREKYRDRIYGV
                     YGFVDAFNPHTGWIDSDVIGINVGIILLSAENMRTGNVWRWFMRNREIPLAMKKIGLL
                     RYSKPNRIAQPQRRKAA"
     gene            4065..>4580
                     /locus_tag="VFV61_05020"
     CDS             4065..>4580
                     /locus_tag="VFV61_05020"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEX4899872.1"
                     /translation="MEFRDRRFRSIAHKLTAALLFSLASLFPLPAQPQNQTQDNTRPR
                     ARDLGINVGILPTGPLNAITDVRGVAVGHTTIIRGENVRTGVTAVLPHQGNLFREKVP
                     GAVFVGNGFGKLMGSTQVNELGEIETPILLTSTLNVPRVADALLDFVLMQPGNEDAQS
                     VNALVGETNDGY"
BASE COUNT         1058 a         1212 c         1236 g         1074 t
ORIGIN      
        1 gctttcgcgc atgacgcttg aagaaaagct tggtcaactg cagcagctcg atggcgaggc
       61 caatgggaat ttccgccccg agcatcgtga tctggtcaaa aaaggattgc tcggttcaac
      121 ccttaacgtc cgcggagtga ccagaaccaa cgagctgcag cgaatagcaa ttgaagggtc
      181 ccgcctaaag attccgttac ttttcggttt cgacgtcatc cacggctacc gcaccatctt
      241 tccgattcct ctcggtgaag cagccacctg ggatccggta gcagttgagc ggagtgctgc
      301 cattgctgcg aaggaagcga gagcgtctgg agttcattgg accttcgctc caatggtcga
      361 catcgcccgc gacgctcgct ggggaagaat tgctgaaggc tccggcgagg atccgtttct
      421 gggatcggta atggctcgcg cccgtgtatt gggatttcaa ggcagtgact acagtgcccc
      481 tgacaaagtg gtggcgtgcg ccaagcattg ggtggcctat ggtgcggcag aaggcgggcg
      541 cgattacaac accacggatc tttcagaaag aactctgcgc gaagtttact ttcctccctt
      601 caaggccgcc ctcgacgccg gtgtgggcac cttcatgagc gccttcaacg atttgaacgg
      661 tgtgccaacc tctgccaatt cattcacgct gactcaggta ttgcggcgag agtggaaatt
      721 tgacggcatc gttgtcagtg actacgaatc agtcaaggaa ctcatcaacc acggactcgc
      781 tgccaacgag aaggaggcag ccgcagcagg actgaatgca ggcgtggaca tggagatggt
      841 gagtcggctt tacaaccagc atggtgcgga cctgctaaaa gagcgaaaac tctcaatggc
      901 gactattgac gaagcagtgc gtcgcattct gcgcatcaag ttccgactcg gactatttga
      961 gaaaccttac gcggacgaag tgcgggagcg taattcggta cttactaagg aacatctcag
     1021 cgccgcccgc gaaagtgcgg ctcggtcgtt ggtcctccta aagaacgaag gcaacgtgtt
     1081 gccgctgagc aagaccttga aggccattgc agtagttggc ccgctggcgg atagccagaa
     1141 agacgtgatt ggctcgtgga caggcgacgg taagccagaa gacgcagtta ctttgcttgc
     1201 tggtattaag tcaaagttat ccccgcagac gatcgtaacc tatgcgaggg gctgcgaaat
     1261 caatgacgac tccacttccg gatttgctga agccgttcgc attgcccgcg agtctgacgt
     1321 tgtcatcgta gccgtgggtg aatcggcgga gatgagcggc gaggccgcgt cgcggagttc
     1381 cttggatctt ccgggccgtc agttggatct ggtaagggct ctgcaagcca ccggcaagcc
     1441 tgtcgtcgtt gtcctgatga atggccggcc gttaagcatt aattggatcg ctgaaaatgt
     1501 tcctgcgatc ctggaaacct ggtttgccgg cacgcaggct ggtaatgcga tcgccgatgt
     1561 catattcggt gatgtgaatc ccggcgggaa attgcccgtc acatttccac gctcggtcgg
     1621 ccaggtgccg ctctactaca atcacaagaa cactggccgg ccaccggatg caaataacaa
     1681 gtacacctct aaatatctcg accaaccgtg gacaccattg ttccccttcg gttatggact
     1741 cagctacacg cggttcaaat tcagtaacct gcaactaagc gcccgacgca ttagacctga
     1801 cggcagtttg acggtgacag tggagttaga gaacgttggc cagcgcgcag gcgatgaagt
     1861 ggcacagctt tacatcaggg atgcggctgc aagcgtcacg cgtccagtaa aagaattgaa
     1921 aggatttcag cgtatcactc tgcaaccggg cgagaagaag cgtgtggaat tcaaacttac
     1981 ttcgcgcgaa cttggctttt atgaccgcga aatgcggttc ttggtagagc ccggtgcggt
     2041 taaagtcatg gttggttcaa gctcagagga cctaatcact gcaatctttg agattgaaaa
     2101 accctgaggc acttaccgac tggcataagg agacaaggtg aagccacaaa aattaaccgt
     2161 gggcttgttc ttgttcgcgg ggctgctctt tctcatcgcg ggattaaggg acattttcgc
     2221 tcccggcttt tttaatgtta gcccgcaagt taaaagcagg ggcgacataa tctttgaatt
     2281 tgctgccgcg gtgacctttc ttaccgtcgg ttatgcgatt agcctgaaca agcgcgttga
     2341 cagcaacaag aaacagtagt tggcaacgat aatctgatgc ccgagaaaat gtcctcagct
     2401 agagtcaggc catccggttt gctttctatc ttcgtaatct tctcacttct cttttcttgc
     2461 tctccaactt tcgcgcgtca gccaatttcg aaagcagtct ctcttcatct tcagggcgct
     2521 ccagttttcc gtctctcacg ccaggacgag gtcttcagtg aagacctcca gcgacgttcc
     2581 tttcagtact tttgggagca ggcggatccg caaacaggcc tggtacccga ccgcgcgcga
     2641 atggatcgtg cgccgctcgc tgagagtcat cgcaatgttg cgagtatcgc ggccacaggt
     2701 tttggtttga ccgctctttg catcgctgcc gagcgagagt ggataacttc gcaacaggct
     2761 gcggaacgaa cgcgtaacac acttcgcttt tttgcccaaa aggcttttca agagcgtgga
     2821 tggttctatc attggctgga cgcgaaaacc ggggagcgaa aatggaatag cgaagtatcc
     2881 tccatcgata ccgccctctt gctggcaggg gtactaacag cgcgtcaata ctttcgcgcc
     2941 gatcccgaaa tcgttaatct cgccaacaga atctacgagc gagttgattt caaatggatg
     3001 ctcaacggag atccgtttct gctctcgcac ggctggaaac ccgagagcgg ctttctaaaa
     3061 ccccgctggg atacgtacag cgaagacaca attctttatt tgctggcgat cggatcaacg
     3121 acacatgcga tcccacctgc gtcctggtat gccttctggc gcgaccgtta tcgctacgaa
     3181 ggacatagct atttcacaac gatcggcgtg cccatattca tgcatcagta ctcgcatgcc
     3241 tggatcgatt ttcgtaaccg gcgtgaggtt aaaggtgatc gcatcgacta ctttgagaat
     3301 tcaatcagtg ccacgcttgc ccatcgcgca ttctgtatca atctcgctgg agagtttcga
     3361 gagtttggac caaacgtctg gggcataacg gcctcggata gtgccaaagg ttatctcgcc
     3421 tggggtggac ctccgcgaga tccggcgatt gacggaaccg tggtaccttc cgcggccggc
     3481 ggttcattaa tgctaacacc tgacatctcc gttgcagcct tgcggactat gcgcgagaag
     3541 tatcgtgatc gaatttatgg ggtttatggt tttgttgatg cgttcaatcc gcacacgggc
     3601 tggatcgaca gcgacgtaat cggcatcaat gtcggcatca tccttttgag tgctgagaac
     3661 atgcgaacgg gcaacgtctg gcgctggttt atgcgaaatc gtgagatccc gctagcaatg
     3721 aagaagattg gattgctgag atacagcaaa cccaatcgaa tcgcgcaacc gcagcgtcgc
     3781 aaagcggcct gagatcgatt ggcgattggc aattgccaat tgccgatttg attggctcgt
     3841 tgcgctacga accaatcagc aatcggaaat cgccaatcgc caatcccttc gcttgtcact
     3901 tctcaattgt cattgtatct ttcagactac gcagttcgga ataaccatga aagttcaaaa
     3961 ctggattagg aaaatgggtt tctggggatt catgttcttc ctcgtcaagg gtctgctttg
     4021 gctcactgta cctgccctca ttgcgctctt tgcgcaataa gctgatggaa ttccgcgacc
     4081 gccgtttccg cagcatcgct cacaagctaa ccgccgcttt attattttcg ctcgcctcac
     4141 tattcccttt accggcacaa cctcaaaatc aaactcagga caacaccaga ccccgagcgc
     4201 gcgacttagg aattaacgtc ggcatcttgc ccacaggtcc gcttaacgcg atcaccgatg
     4261 ttcgcggggt tgcggtagga catacgacta tcatccgcgg cgagaatgtg cgtacaggcg
     4321 taacagccgt actgccacat cagggcaacc tctttcgtga aaaagttccc ggcgcggttt
     4381 ttgtggggaa tgggtttggc aaactcatgg gttcaactca agtcaacgaa ttgggcgaga
     4441 tcgagacgcc aattctcctc acttcaacgc tcaacgttcc gcgcgtagcc gacgctctcc
     4501 tggatttcgt tctcatgcag cccggcaacg aggacgcaca gtcggtcaat gcgttggtcg
     4561 gggaaactaa cgatggctac
//
LOCUS       DASUEX010000300         4604 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1184522, whole genome shotgun sequence.
ACCESSION   DASUEX010000300 DASUEX010000000
VERSION     DASUEX010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4604
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1184522"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..1508)
                     /locus_tag="VFV61_05025"
     CDS             complement(105..1508)
                     /locus_tag="VFV61_05025"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX4899873.1"
                     /translation="MSDQQAARILIAEDEANLRLVLQKELERLGYRVQAAPDGEAALR
                     KLEESNVDVLLCDINMPRIDGMEVLRRVHERPNPPEVIMLTGQATVETAVEAMKLGAY
                     DYLTKPYRIAELDVRVRQAVEKRRLRVDNLRLREQLARQSALPEIVSVSDVMKEAIRL
                     VERVAPSEASVLITGESGTGKELIAHAIHRLSNRANASFIDLNCAAFQESLLESELFG
                     YEAGAFSGAKGRKLGLIELSDGGTLFLDEVTELPAQLQAKLLRAIETRTFFRVGGVRT
                     VEVDTRIVAATNRNLESVVADGSFRSDLLYRINGFQIDLVPLRDRREDIEPLARHLLQ
                     QVGGPATPELDDSALDALRSYSWPGNVRQLKNTLERAVILSNNGRITLAELPPEVARP
                     GVYAPALVTTSLASATEAGANPVGSPASLREVERQQILAALEQSGWHRGKTAEILGIS
                     PSTLYRRLRDYGLEKRR"
     gene            complement(1505..3556)
                     /locus_tag="VFV61_05030"
     CDS             complement(1505..3556)
                     /locus_tag="VFV61_05030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX4899874.1"
                     /translation="MSAPATTVPENVTLLIVCAESGVRGRLEAIFKTAGYRTLATADA
                     SAALSVLHKEHCDLVVLDLEIEGVDGLALCRLLRSQTATSKLPIIALSNDDLESTEVE
                     AFAAGADDYIIKPSSPGELISRVNAQLKSAQREWELIGSNRELSFLAELGRGLLRALE
                     PDQLVRRIAGATYEGTGAVLCAAYVTLSDDCEAGCVFDREGSAEDASHLYRERLQSWL
                     ELPTTTTPERLTDRKSFFLRDEVHEIEYAAPLRFGGRAKGVLVVAFDRREDLGETESR
                     LIDAAAQQAALAAHISSLYQAARETSTNLAKEVDRRTAEAFAQRRFTEAIIDSLPLSL
                     YAVDSEYRIVAWNRNRELGELGIPRGAALGRNIFEVLTLQKREVLEPEFEQVFATGEM
                     EVIEQETVKPNGEIKHWLISKIPMWIDDSGKVSHVITVGEDITARVEANRGVARAEKL
                     AAVGRLAAGVVHEINNPLATISACAEALESRIADGSFAESPALDDLREYLSLIRNEAF
                     RCKSITTGLLDFSRTRTAQHVLLDLSDVINSTAKLLVHQQRGAQIDLRIETSDNLPPV
                     MGDPGQLQQAIITLATNAVDAMQAGGVLTISSRSEGKNLLVEVSDTGVGIPPENIAKI
                     FEPFFTTKEVGQGTGLGLAVCYGILTEHGGSLDVQSAPEAGTTFTITLPAAIEFGEAN
                     V"
     gene            complement(3736..4374)
                     /locus_tag="VFV61_05035"
     CDS             complement(3736..4374)
                     /locus_tag="VFV61_05035"
                     /inference="COORDINATES: protein motif:HMM:NF013695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEX4899875.1"
                     /translation="MSSEVLRSESLRRGRVLEYLTIIWNLLEGIVSVGAGLLAGSIAL
                     VGFGFDSFIESLSGGALLWRLHLDAPERRERAEYIALKLVGISFLLLAAYVAFDAIKS
                     LIQREPPEASYIGIGIAALSLVVMPFLARAKSKVAGELNSRALQADSRQTDICAYLSA
                     ILLGGLLLNAIFGFWWADPVAALVMTPIIAREGIEALRGETCDDCHAVSTDT"
BASE COUNT         1041 a         1330 c         1209 g         1024 t
ORIGIN      
        1 cagcgaactt aagtttcgct gccttcattc tttgcgtgaa cctgttattt ttatcccagg
       61 cttctgccgt ctgctgctgt ctactgtctg ctgtcgactg tctctcagcg ccgtttttcg
      121 agaccgtaat ctcgcagacg ccggtagagt gttgaagggg agattcccag aatctcggcg
      181 gtctttcccc ggtgccatcc agactgttcc agggccgcga gtatctgttg gcgctcgacc
      241 tcgcgcaggg atgctggcga gccgactggg tttgccccgg cctctgtagc actcgcgagc
      301 gatgttgtca ctaaggccgg cgcatagacg cctggacgag ctacttccgg aggcaattca
      361 gccagagtaa tacggccgtt attggaaagg atcacggcgc gttcgagtgt gttctttagt
      421 tgcctgacgt tgccgggcca actgtaggag cgcaacgcgt cgagggccga atcatccaac
      481 tccggcgttg ctggtccgcc aacttgttgc agtaaatggc gggcgagcgg ctcaatgtct
      541 tcgcgtcgat cacgcaacgg caccaggtcg atctgaaatc cattgatgcg ataaagcaga
      601 tcggaacgaa atgaaccatc agctacgact gactcaaggt ttctgttcgt cgctgcgacg
      661 atacgcgtgt cgacttcaac cgtgcgtacg ccaccgacgc ggaaaaaagt cctggtctcg
      721 atcgcgcgca acaatttggc ctgcaactgc gcgggaagct ccgttacctc atcaaggaag
      781 agtgtgcctc cgtcggaaag ctcaatcaaa ccgagctttc gccccttggc tccggaaaat
      841 gcgccggcct cgtatccgaa gagctccgat tccagcaacg actcttgaaa agctgcgcag
      901 ttgagatcaa tgaacgatgc atttgcgcga ttcgaaaggc gatggatcgc gtgggcaata
      961 agttccttgc ccgtccccga ctcgccggta atcaaaacgg aagcctcgga cggcgctact
     1021 cgctcgacca agcgaatcgc ttccttcatc acatctgaaa ccgagacgat ctcagggagg
     1081 gctgattgcc gcgccagttg ttcgcgcaag cgcaagttat caacgcgcaa gcggcgtttt
     1141 tccacagcct ggcgcacgcg cacatctagt tccgcgattc tgtatggctt agtgaggtaa
     1201 tcgtaggcgc ctaacttcat cgcctcgacg gccgtttcga cagtcgcctg acccgtcagc
     1261 atgatcactt ccggcggatt tggtcgctcg tgaactcgtc ttagcacttc catgccatca
     1321 atgcgcggca tgttgatatc gcagagcaac acatccacgt tactctcttc aagcttgcgc
     1381 aaggctgctt cgccatcagg cgccgcctgc acacgatatc cgagtcgctc gagctccttc
     1441 tgcaaaacta gtcgcaggtt agcttcgtct tcggcgatca gaatccgtgc tgcttgttgg
     1501 tcgctcaaac gttggcttct ccgaattcaa ttgctgccgg caatgtgatc gtgaaagtgg
     1561 taccggcttc aggcgctgat tgcacgtcaa gactcccgcc atgctcggtt aagataccat
     1621 aacaaacagc gagaccaagt ccagtgccct gcccgacttc tttcgtggta aagaacggtt
     1681 caaagatctt agcgatgttt tcgggtggta tccccacacc agtgtcactc acctctacta
     1741 acaagttctt gccttcagaa cgcgacgaga ttgtcagcac gccgcccgcc tgcattgcat
     1801 cgacggcatt cgtcgccaag gtaatgatcg cctgctgcaa ttgaccaggg tcccccataa
     1861 cgggtggcaa attgtcagag gtttcgatcc gcaaatcgat ttgggcgccg cgttgctgat
     1921 gcaccaggag ctttgccgta gagttaatca catcagacaa gtcgagcaat acgtgctggg
     1981 ccgtgcgcgt gcgactaaaa tccaacaggc cagtggtgat tgacttgcaa cgaaacgctt
     2041 cgttacgaat taaactcaaa tactcccgca gatcgtccag cgccggcgac tcggcaaacg
     2101 agccgtcagc aatcctcgac tcgagggcct cagcacatgc ggaaatcgtc gccaggggat
     2161 tgtttatttc atgcactacg ccagccgcaa gccgtccgac agccgcaagc ttttccgcgc
     2221 gtgcgacccc ccggttagct tctacacgcg ccgtgatgtc ttcacctacg gtgataacgt
     2281 gagagacctt gccgctatcg tcgatccaca tcgggatctt gctgatcagc caatgcttga
     2341 tttcgccgtt aggctttaca gtctcctgtt cgataacctc catttctccg gtagcaaaaa
     2401 cctgctcaaa ctccggctcc agcacctcac gtttttgcag cgtcagcacc tcaaaaatgt
     2461 tgcgaccaag cgcagcgccc cgcggaattc ctaactcacc caattcgcga ttgcgattcc
     2521 aggcgacaat ccgatattcg ctgtcgacgg cataaagcga caaaggaaga ctgtcgatga
     2581 ttgcttcggt gaagcgtcgt tgcgcgaacg cttcggcggt ccgccgatcg acttcctttg
     2641 ccaggttagt cgaggtctcg cgggccgcct gatacaggga cgaaatatgc gcggccagcg
     2701 ccgcctgttg cgcagcagca tcgattagcc tgctttcagt ctcgccgaga tcctcacgac
     2761 ggtcgaacgc taccaccagc acgcccttag cgcgtcctcc aaacctcaag ggcgcggcat
     2821 attcaatctc gtgcacttca tctcgcaaaa agaaactttt acgatcagta agccgctcag
     2881 gcgtcgtagt cgtgggcaac tccaaccagc tttgtagtct ttcacgatag agatgcgacg
     2941 catcctcagc gctcccctca cggtcaaaca cacatcccgc ttcgcaatca tcagacagcg
     3001 tcacatacgc cgcacacagc acagcgccgg tgccttcata cgtggcgccg gcgatccgtc
     3061 gcaccaattg atcgggttcg agtgcgcgca gcagcccgcg acctaactca gccaggaaac
     3121 tcaactcgcg attactgccg attagttccc attctcgttg agccgattta agctgagcat
     3181 taacgcgaga gataagttcg ccaggtgacg aaggtttgat gatgtagtcg tcggcgcccg
     3241 ccgcaaacgc ctcgacctct gtgctttcga ggtcgtcgtt tgagagcgca attattggaa
     3301 gtttgctggt ggcggtttgc gaacggagca ggcggcataa cgccagtcca tcaactcctt
     3361 caatctccag atccaggaca acaagatcac aatgctcttt atgaagcaca ctgagggcgg
     3421 ccgaggcatc ggctgtcgcc agggtacgat aaccggcagt cttgaagatg gcttcgagtc
     3481 tgccgcgcac accgctttcg gcgcagacaa tcagcagagt tacattttca ggaacggtgg
     3541 tggcgggcgc agacatgctt taatcgtagc tgggccagcg ggctggcggg tgggacctgg
     3601 gtggccgtgt tttcggcatg aatgccaaga acacgccgcc ccactagcga ttgaaagcga
     3661 tgggggatga gaatgtcaag acttccagtc agggagcgaa atcgagcttg gttctttgta
     3721 ctttgaactt cgggactagg tatctgttga aacagcatgg cagtcatcgc aggtttcccc
     3781 tcgtagagct tcgattcctt ccctcgcaat gatcggagtc atcaccaaag ccgctacggg
     3841 atcagcccac caaaagccaa agatcgcatt caacagaaga ccgccaagca ggattgccga
     3901 caggtaggcg catatgtccg tctggcgtga gtcggcttgt agtgcgcgac tgtttagttc
     3961 accagccacc ttactcttag ctcgcgcaag gaacggcatc acaactagag acagcgcggc
     4021 aatacctatc ccgatgtaac tagcttctgg tggctcgcgt tgaattagag acttgatcgc
     4081 atcgaaggct acgtacgcgg caagcaaaag gaaactgatt ccgactagct ttagggctat
     4141 atactccgct cgctcgcggc gttctggtgc gtccagatgc aagcgccaca gcaacgcgcc
     4201 accagagagg ctttcaataa acgaatcaaa gccaaagccg actagcgcaa tgctgccagc
     4261 gagcaagcca gcgccaacgg agacgattcc ttctaagaga ttccagatga tggtgaggta
     4321 ttcaagaacg cggccacgtc gcagggattc agaacgtaag acttcgctac tcattgactg
     4381 agtagcttac caacatcgct gagcggttaa caaccagata aggtggacca cctactgact
     4441 cccgataaaa tcaaacgctt gccatcggag cactaagttc taagtattag ttccgtggtt
     4501 caattgatag aaatagttgt caggccatca ttgttgagat tgggacaaca agctcggctg
     4561 tgctcgcggc gttaagcttc accagtttgt gagacgactt cact
//
LOCUS       DASUEX010000301         2944 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1093177, whole genome shotgun sequence.
ACCESSION   DASUEX010000301 DASUEX010000000
VERSION     DASUEX010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1093177"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..717
                     /locus_tag="VFV61_05040"
     CDS             <1..717
                     /locus_tag="VFV61_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899876.1"
                     /translation="NYTFSKSIDDASGLQTSGVFGAAFITNALRQRDNRAASDFDIRH
                     VVNFNSIWELPFGRGRKYFTGANKAVDAVLGGWVLTSIFRYNSGLPVSSPVDLGGWPT
                     NWNVRSWATPIRAIQSSPSRNTNGRPNLFADPEAAYQSFRSPAPGETGARNILRGMHY
                     FTLDAGLYKSFNLPWSEKQKITIRWETFNVTNSQFFDGNADVTNGLDPQFGTPSPTFY
                     NFTRIQGNPRIMQFALRFDF"
     gene            831..1838
                     /locus_tag="VFV61_05045"
     CDS             831..1838
                     /locus_tag="VFV61_05045"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX4899877.1"
                     /translation="MTIRKLLIIALIFALHGLALGQGSNSSQPGAGTRRNFTLFGDLK
                     VDDSQVDRGANTNVTFDVILYTRGNEVFARQRVGNGGRYQFNNVFNGDYYLAVELDSA
                     EIARMQIMIPANAVEHIRQDLEFRWKPTMGAAPKTPADTYNRNSQNRTLYQRAAHEID
                     SKNFTQAIVTLRSVVEADPKDYPAWTDLGMVYFLQKDLEAAENSYNSAIQAKPDHVAA
                     LVSLGRVRLARKNNEGAIEPLEAALKVDPKSATANYFLGEAYLALKKGSKAVGYLNEA
                     LKIDPIGMADAHLRLGALYNVAGYKDRAAAEYEQFLQKKPDYPEKKKLMDYIEANKPK
                     P"
     gene            2147..2398
                     /locus_tag="VFV61_05050"
     CDS             2147..2398
                     /locus_tag="VFV61_05050"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX4899878.1"
                     /translation="MGRSYRSRPRRLAVKLKAVRMKLGLTQTDMVKALAVKDEPLRAA
                     SISGYELGQREPPLLVLLRYARLGGCTMEQLVDDKIKWP"
     gene            2593..>2944
                     /locus_tag="VFV61_05055"
     CDS             2593..>2944
                     /locus_tag="VFV61_05055"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX4899879.1"
                     /translation="MSLVLSLIGFAALAMIVHELGHLLAARSCHISASELGLGMGPRL
                     AGFRLGSITFNLRAIPIGSFVMLDGTALKEKSLKAQLLVHLGGVIFNLVAGFFTYGTT
                     FGWLNLLLAAGNILP"
BASE COUNT          684 a          804 c          797 g          659 t
ORIGIN      
        1 aattacacct tctcgaagtc gatcgacgat gcttccggac ttcagaccag cggcgtcttc
       61 ggtgcggctt tcatcaccaa cgcactgcgt cagcgtgaca accgtgccgc ttcggacttc
      121 gacatcaggc acgtcgtaaa cttcaactcg atttgggaac ttccatttgg acgcggtcgc
      181 aagtacttta ccggcgcgaa caaggctgtg gatgccgtgt tgggtggttg ggtgctcact
      241 tcgatcttcc gttacaacag cggattgccg gtaagctctc cggtggacct cggtggctgg
      301 ccaacgaact ggaacgtcag aagttgggca acgcccatcc gcgcgatcca gtcgtcgccg
      361 agccgcaata ctaatggaag accgaacctg ttcgcggatc cggaagctgc ttatcagagt
      421 ttccgtagcc ccgcgccggg cgaaacggga gccagaaaca tcctgagagg tatgcattac
      481 ttcactctcg atgccggcct ctataagagc ttcaatttac catggagcga aaaacagaag
      541 attacgattc gttgggaaac attcaacgtt accaacagtc agtttttcga cggcaacgcg
      601 gacgtaacaa acggtctcga tccgcagttc ggaacgccga gcccaacgtt ctataacttc
      661 accagaattc aagggaaccc aagaatcatg cagttcgcac tgcgattcga cttctagtgg
      721 cgacctgagt ttggtccatt cacgcgggct gggacttcgg tcacagcccg cgctttcttg
      781 cagtcgcccc ggcattaccg ggctcagctt ctcttgaatc cggaggtgtt atgacaattc
      841 gaaagctgtt gataattgca ctcatatttg cattgcacgg tctcgcgttg ggccagggta
      901 gtaattcatc acaacccggc gctggaacgc gtcgcaactt tacgctcttc ggcgatctga
      961 aagttgatga ctcgcaagtt gaccgtggcg ccaacacgaa tgtgacgttc gacgtgattc
     1021 tgtacacgcg cggaaacgag gtgtttgcgc gacaacgcgt gggtaatggc ggacgttatc
     1081 agttcaataa cgtttttaat ggcgactatt atctcgcggt ggagctcgat agcgccgaga
     1141 tcgcgcgcat gcagatcatg attccggcaa acgcggtcga gcacattcgt caggatctcg
     1201 agttcaggtg gaagccgacg atgggcgccg ctccgaaaac gcctgctgac acctacaaca
     1261 gaaacagtca gaatcgcacg ctttatcaga gagcggctca cgagatcgat agtaaaaact
     1321 ttactcaagc catcgtcacc ttgcgttccg tggtggaagc tgacccaaaa gactatcccg
     1381 cgtggacgga tctcggcatg gtctatttct tacaaaagga tcttgaagcc gctgagaaca
     1441 gctacaacag tgctatccag gcaaagcccg atcacgtggc ggcactcgtg agcctcggac
     1501 gcgtgcgcct cgcgcggaag aacaacgagg gcgcaatcga gccgttggaa gcggcgttga
     1561 aggttgatcc gaagtctgcg acggcgaact actttttagg tgaagcttat ctcgcgctca
     1621 agaaaggctc gaaggcagtt gggtatttga atgaggcgtt gaagatcgat ccgatcggca
     1681 tggccgatgc gcacctgcgg ctaggcgctc tctacaacgt cgccgggtac aaggatcggg
     1741 cggcggctga gtacgaacaa tttctgcaaa agaagcctga ttatccggag aagaaaaaac
     1801 tcatggatta catcgaggcg aacaaaccaa aaccctaatt gtcagtacca cctgcgattg
     1861 cgggtgggta aaagaaagag cggcagcttc acccgcaaaa gtagctgccg ctctcgtcag
     1921 tgaactactg tgatggctta ggccattctg ggccacgtgc tcaccgaaat gctaaaatcc
     1981 gcaattgtga attatctgag aggtaaaact ggctgatgcc gcccgccctg atgcaaggcg
     2041 tcagggcgac tcgaacggca cggcgaatcc taacactatt tagtgaagtt aacaaggtag
     2101 cagacagcgc ctcatcgcgc tgttcctcag aagggacagt ttgaggatgg gtcgctccta
     2161 ccgctcccgg cccaggcggc tggccgtaaa gctgaaagcc gtcagaatga agcttggact
     2221 gacgcagacc gacatggtca aggcgctcgc agttaaagac gaaccattac gcgccgccag
     2281 catctccggt tacgagctcg gccaacgcga gccaccactc ctggtcctgc tcagatacgc
     2341 gcggctggga ggctgcacga tggaacaact ggtcgacgac aagatcaagt ggccctaaaa
     2401 actcctttat ccacagatta cgcagattgt ttataacttc tgtgtaatct gtggatggct
     2461 ttgcctgaga cattctttgt ctgttttcca gtcacaattt tcgtcacttc ctccggactt
     2521 tcctcagaaa acctcagtaa atccaatcgg cataggtctt gacactcgtc ccgcgggacg
     2581 gtagcagact tgatgtcgtt ggtattgtca ctcataggat ttgctgcgct ggcgatgatt
     2641 gtccacgagt tgggccatct gctggccgcg cgctcctgcc acatctccgc ctctgagttg
     2701 ggtctcggca tggggccacg actcgcgggt ttccgtctcg gaagcatcac cttcaacttg
     2761 cgcgcgatac caatcggttc gttcgtgatg ctcgatggca ctgcgttgaa agagaaatcc
     2821 ttgaaggctc agctcctcgt gcacctcggt ggcgttatct tcaatctcgt cgccgggttc
     2881 ttcacctatg gaacgacttt cggctggctg aatctactgc tagccgcggg caacattctt
     2941 cccc
//
LOCUS       DASUEX010000302         3574 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_735830, whole genome shotgun sequence.
ACCESSION   DASUEX010000302 DASUEX010000000
VERSION     DASUEX010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3574
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_735830"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(515..1018)
                     /locus_tag="VFV61_05060"
     CDS             complement(515..1018)
                     /locus_tag="VFV61_05060"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX4899880.1"
                     /translation="MKNKDPLATAGLPALNRNNEDQSVSGDVIAEGGQQSEGKFLILV
                     ADDSADNVAMISLDLQQQGYRVVTASNGEDAINVASQTMPNLILMDISMPTLDGLGAT
                     RRIRDDAALRDIPVIAVTAFGTEGFQRAAYDAGVSGYLTKPINLDRMHQLIARLLAPG
                     GSGNLSS"
     gene            1151..1633
                     /locus_tag="VFV61_05065"
     CDS             1151..1633
                     /locus_tag="VFV61_05065"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX4899881.1"
                     /translation="MKNLQLTQAPICKAEMLIRRPAAEVFEAFVDPEITKKFWFTKST
                     GRLQAGKHIVWTWEMYNMSIDVEVKEVEPNKRILIEWGSYGAMTMVEWIFAPYENGST
                     YVTITNSGFQGDGDKVVNDALDSKGGFTWVLAGLKALLEHNIKLNAIADAYPQGHKRD
                     "
     gene            complement(1723..>3574)
                     /locus_tag="VFV61_05070"
     CDS             complement(1723..>3574)
                     /locus_tag="VFV61_05070"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEX4899882.1"
                     /translation="QVGVFKYVVPISIGIGVLLVIVALSYRQTIHAYPSGGGAYIVAK
                     ENLGTNAGLVAGASLLVDYVLTVAVSIAAGVAAITSMAQGTRYAWLDHHKVFLCLLFI
                     AFIALANLRGVRESGALFAAPTYAFLVSFLFMIGYGLYSYYTYGGAAPIPGPEEIKTA
                     EGYKIQPLTLFLLLGAFSNGCAALTGIEAISNGVPAFKKPEARNAATTLIIMAALLTI
                     MFLGTSVLAYLYGVHPHTSETVISQFARIMFKGPMAWFYYVVQIATALILVLAANTSF
                     ADFPRLGSLLARDRFLPRQFATRGDKLVFSNGIVILAIFASILVIAFAGDTSRLIPLY
                     AVGVFLSFTLSQSGMIRHWLKVRAQAPKNKVKRGRRPEDDIHFTQSTVDREPLPDAEL
                     EASEQRGSTFVTDEVTDSTHWKKSIAINALGAVATFIVLCVFIATKFIHGAWIVVVVI
                     PLLIMMFRAIHTHYVGVAKQLSTEGLEELPPLKHTVIVPISGIHRGVVSALQYAKSIA
                     PDHVQAVYVDFDQEATVKLREKWERWGAGVKLVVLPSPYRELTRPLLRYISRLERRNH
                     NDVVTVLLPEFVPAKWWQHLLHNQSSLMLKGALLFKEGVIVISVPYHLKG"
BASE COUNT          890 a          960 c          961 g          763 t
ORIGIN      
        1 tacatggata gcgatcgcga gccgcagcgt ccaaagcctc aatcgcaccc ccaatcaaag
       61 cctcaggatc gtgagggccc gcgctcgcca cgcatgatgg catttggcga gacggtaaag
      121 tgcgcctcat gcggagcgaa ggctccgccg aagatcaacg ccgacagctc ttgcccaaag
      181 tgcaacgccg atcttcacag ctgccgccag tgcacctatt tcgatccggg cgcccgcttc
      241 gaatgcagta aaacgatcac cacacggatc atgaacaaac acgctcgcaa cacatgcgaa
      301 ctgttcgcgg ctcgaatcgt agttgagcgc gagacctctt caggccctcc gacggatgct
      361 cgtcaggcat tcgcaaaatt atttaagaag taaaatgttt gcgggtagta gaggcggcca
      421 ataaccaggg gtcgcgcgtt tcgtgttgct tcgtgggatc ggtttgctcc cgacacacga
      481 aagatagctg tggaattcga tgttggctaa aggtttaact gcttaagttc cccgatcctc
      541 ccggcgcaag gagccgggca atcagttgat gcatacgatc cagattgatc ggtttggtta
      601 aataaccgga aacgcctgcg tcgtaggctg cacgctgaaa cccttcggtg ccaaacgcag
      661 taacggcaat cacgggtatg tctcgcaatg cggcatcgtc gcgaatccgg cgtgttgcac
      721 ccagcccgtc cagagtcggc atgctgatgt ccatcaaaat caggtttggc atcgtctgtg
      781 atgcaacgtt gatcgcgtcc tcgccattac tggctgtcac aactcggtaa ccctgttgct
      841 gaagatccaa gctgatcatt gccacgttat cagccgaatc gtcagcaaca aggatcagaa
      901 acttcccttc tgattgttga cctccttctg ctatgacgtc tcccgagacg ctttgatcct
      961 cattattgcg attcaatgcc ggaagccctg cagtcgcaag cgggtctttg tttttcatat
     1021 gccgcgatta tagccatcat cacggccgtt acctaaaacg agctgatagg aacgtctcag
     1081 gtttaggatc tccaacaagg ccccgacagg caacgctgac gatctaatac gtatggaata
     1141 aaatggaagc atgaaaaatc ttcaactaac gcaagcgcct atttgcaagg cggagatgct
     1201 tattcgccgg ccagcggcgg aggtgtttga ggcttttgtc gatccggaaa ttacgaagaa
     1261 gttttggttc acaaaaagta ccgggcgatt gcaagcgggc aagcacatcg tctggacatg
     1321 ggagatgtac aacatgtcca tcgatgttga ggtaaaggaa gtcgaaccaa acaaaaggat
     1381 cctgattgag tggggcagct atggcgcaat gacgatggtc gagtggatct ttgctcctta
     1441 tgaaaacggg tcgacgtacg tgacgatcac aaattccggt ttccagggag atggtgacaa
     1501 ggtggtgaat gatgcgcttg attcgaaggg tggatttacc tgggtgcttg ctggtctgaa
     1561 agcactgctg gaacacaaca tcaagttgaa cgcgatcgca gatgcttatc cgcagggtca
     1621 caaaagagat tagattcagg gtggatcacg tttcgtgttt attcgtgcgt cccctgttgt
     1681 ctggtcaaat agaaacgatc cacgaagcaa cccgaaaact gatcatcctt tcagatggta
     1741 tggcacactg atgacgatca cgccttcctt gaagagcagc gcacctttga gcattagtga
     1801 gctctgattg tgtagcagat gttgccacca ctttgccggc acaaattccg gtaacaacac
     1861 agtcaccaca tcgttgtggt tccttctttc cagccggcta atgtagcgca gtagaggtcg
     1921 ggtcaactcg cgatacggcg acgggagcac caccagtttc acgccggcgc cccagcgctc
     1981 ccacttctca cgtaacttca ccgtcgcctc ctgatcaaag tccacataca cagcctgaac
     2041 gtggtccggc gctatcgact tggcgtattg cagcgctgag accacgcccc ggtgaatacc
     2101 ggagatcgga actatgactg tgtgcttgag tggtggaagt tcctcgagac cctccgttga
     2161 gagttgtttc gcgacgccaa cgtagtgtgt atggattgcc cgaaacatca ttatcagcag
     2221 cggaatcacg accacaacta tccaggcgcc gtgtatgaac ttggtcgcaa taaacacgca
     2281 gagaacgatg aaggtagcga ccgctccgag cgcattaatt gcgatcgatt tcttccagtg
     2341 cgtgctatca gtcacctcat ctgtgacgaa ggtcgaccca cgttgctccg acgcttccag
     2401 ttcggcatca ggcaacggct cacgatcgac agtactttgc gtaaagtgaa tgtcgtcctc
     2461 aggccggcga cctcgcttca ccttattctt cggtgcctgg gcgcgcacct tcagccagtg
     2521 ccggatcatg ccggactggg aaagcgtgaa cgacaggaag acacccacgg catagagagg
     2581 aatgagccga ctcgtgtcgc ccgcaaatgc aattaccagg atgctcgcga agattgccag
     2641 gatcacaatc ccgtttgaga aaacgagctt gtctcctcgc gtcgcaaact ggcgcggcag
     2701 gaagcgatca cgggccagta agcttccaag tcgtgggaag tcggcaaacg aagtgttggc
     2761 ggcgagcacc agaattaagg cggttgcgat ttgcacgacg tagtagaacc aagccattgg
     2821 ccccttaaac atgatgcgcg caaattgcga gatgacagtc tcgctcgtgt ggggatgaac
     2881 gccataaagg tacgccagca cgctggtacc caaaaacatg atcgtcagca gcgcggccat
     2941 gataatcagg gtcgtggccg cgttccgcgc ttccggtttc ttgaaagctg ggacgccatt
     3001 ggagatggct tcgataccgg tcagggcggc gcacccgttt gaaaaagctc ccaacaacag
     3061 aaacagcgtc aagggctgaa tcttgtatcc ttccgccgtt ttgatctctt caggacccgg
     3121 gatgggtgca gctccaccat aggtgtaata actgtagaga ccgtaaccga tcatgaagag
     3181 aaaactaaca aggaaggcgt aggttggcgc tgcaaacaac gccccggact cacgcacgcc
     3241 acgcaagttg gccagagcaa taaaggctat gaacagcaaa caaagaaaga ctttgtggtg
     3301 gtccaaccag gcgtagcgcg ttccctgggc catggaagta atggcggcaa cacccgcggc
     3361 aatggaaact gcaacggtca acacgtaatc aaccagcagc gaggctcccg ctaccaggcc
     3421 cgcattagtg ccgaggtttt ctttcgcaac gatgtaggcg ccgccgcccg atggataggc
     3481 atgaattgtt tgccggtagc ttagagcgac gattaccagc agcaccccaa tgccgatcga
     3541 gatcggcacg acgtacttaa acacacccac ttgc
//
LOCUS       DASUEX010000303         2995 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_159002, whole genome shotgun sequence.
ACCESSION   DASUEX010000303 DASUEX010000000
VERSION     DASUEX010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2995
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159002"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(145..2079)
                     /locus_tag="VFV61_05075"
     CDS             complement(145..2079)
                     /locus_tag="VFV61_05075"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HEX4899883.1"
                     /translation="MHASLQLRQLTLVTRHPTPLLALALLLCSISAAGQQAKTEVQSL
                     PAIASRAEFDSLARIFSDSSYPLSHVLFVVDRRDRNKIYYVNSRRYAFHKDFVNGTYL
                     SLERGQDFFENNYLKANRRFILGTLAYQTPVQRWTFEFWEGDQIPGELIKTTSDVINK
                     TFFTSVAYKPNSSRQEEASASLAGLERVLQSDISQGQEYEPLNLARGLGRIHIIEKLD
                     EHVEIGFNEILVLNEVPIQLPPVAGVIVSKPSTPLSHINLLLKGWGVPNAYIKNAHEL
                     LKQYDGRWVSFETKRDGYTIKPADMDQLREYQKRMSQRLDVVKPRSDLSATRIKTLSQ
                     QRARDVIAYGGKSANLGELMNARLPGMIIPDGFTVPFFYYDQFINENGLDDFIFELLN
                     DQKFVHDPAYRRGRLSELRERIQNGKINEALRTEVLRLAHAKYPGKGLFARSSSNAED
                     LPNFSGAGLHSSVPNVKGDHQLLEALKTVWASLWNFEAYEARERAGIDHAKVYMAVLI
                     QEGINADSAGVMITADPYNPESKGAIYISAKRGLGIKVVEGKRIAEQILFQPASNAIR
                     VLTRSEEDSLLTFDEKGGVKEIPITGARAVLTDEMARRLAFAAQRIRRVFAGKDQDIE
                     WLYLRGQLYIVQARPFIAGN"
     gene            complement(2243..>2995)
                     /locus_tag="VFV61_05080"
     CDS             complement(2243..>2995)
                     /locus_tag="VFV61_05080"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX4899884.1"
                     /translation="SLRSQLQSVIDAGKSIINTNRPGDETFLVRFISSDKIITEQDFT
                     SNQDLLIDALDGLYVEGGQTAVIDAVYLAAERLAEYKKGDDNDRRRRALIVVTDGEDR
                     VSFYKPEQLFARLREQDVQIYVIGFVNELDKEGGFIRKSPRERSVNLINKMASETGGR
                     AFFPQSLSELPQIANEIVRDLRTQYVLAYNPTNKARDGSYRAIRVTVADAPGRDKRIA
                     LTRSGRNAPRGPAPTTRATPPPPRTRLPAAKP"
BASE COUNT          661 a          810 c          817 g          707 t
ORIGIN      
        1 cgccgcaggc gggaattgaa ataacggcgc cactcccatg atcagaaaac ccaacttcgt
       61 tcaacatgac gatcggcaag acagcggcgg caagcccgcc ttccggactg cacgttccat
      121 cgcctgtcgc cctcaggcac agccttagtt tcctgcaata aacggccgtg cttggacaat
      181 gtagagttgg ccacgcaagt agagccactc aatgtcctgg tcttttcccg cgaacacacg
      241 tctaatccgc tgtgccgcga aggccagccg tcgtgccatc tcgtccgtca acaccgcgcg
      301 cgcgcccgtg ataggaattt cctttacacc acctttctcg tcgaacgtca gtaagctgtc
      361 ctcctccgac ctcgttagta ctcgaatcgc gttcgaggct ggttgaaaaa gaatctgttc
      421 cgcgattcgc ttaccttcca caactttgat cccgagaccg cgtttggcac tgatatagat
      481 cgcgcccttg ctttcgggat tgtagggatc ggcggtgatc atcactccgg cgctgtccgc
      541 gttgatgcct tcctggatca agaccgccat gtaaactttc gcatgatcga tgccggcgcg
      601 ctccctggct tcataggctt cgaagttcca gagtgaagcc cagacagtct tcagtgcttc
      661 cagcaattgg tgatcgcctt tcacgttcgg cacactcgaa tgcagacctg cgccgctgaa
      721 gtttggcaag tcttccgcgt ttgatgagct gcgtgcaaat aggcccttgc cgggatattt
      781 agcgtgcgct aaacgcaata cttccgtgcg cagtgcttcg ttgatctttc cgttctggat
      841 gcgttcgcgc aattcgctta gacgtccacg gcgataagcc ggatcgtgaa caaacttctg
      901 gtcgttcagc agctcgaaga taaaatcatc gagcccgttt tcattgatga actggtcgta
      961 atagaaaaag gggactgtaa agccatcagg aatgatcatg ccgggcagcc gggcgttcat
     1021 tagctcacca agattggccg acttgcctcc ataggcaata acatcgcgcg ctcgttgctg
     1081 acttaatgtc tttatgcggg ttgccgacaa gtcagagcga ggtttcacca catcaaggcg
     1141 ctgcgacatg cgcttttggt actcgcgcag ttgatccatg tcggcgggct tgatggtgta
     1201 gccgtcgcgc ttggtttcaa aagagaccca acgaccgtcg tattgcttca gcagttcatg
     1261 ggcgttcttg atgtaagcat tcggcacgcc ccaacctttc aacagtaagt tgatatggga
     1321 aagcggagta gaaggcttac tgacaatcac gccggcgaca ggcggaagct ggatcggcac
     1381 ttcgttcaac accagaattt cattgaagcc aatctcaaca tgttcatcga gcttttcgat
     1441 gatgtgaatg cgacctagtc cgcgcgcaag attgagtggt tcatattcct ggccctgaga
     1501 gatatcactc tgcaggacgc gttcaagacc agccaacgac gcggatgctt cttcctgtcg
     1561 cgaagagttg ggcttatacg ccacgctggt aaagaaagtc ttattgatga catcgcttgt
     1621 ggtcttgatt agctcgccgg gtatttgatc gccttcccaa aattcaaagg tccagcgctg
     1681 caccggagtt tggtaagcga gcgtgccgag aatgaagcgc cgattggctt tgagatagtt
     1741 gttctcaaag aaatcctggc cgcgctccaa tgataagtac gtgccgttca cgaagtcctt
     1801 gtgaaaggca taacggcgag agttgacgta gtagatcttg ttgcggtcgc ggcgatcaac
     1861 tacgaaaaga acgtgcgaga gcggatagga actgtcggaa aaaatgcgag cgagggaatc
     1921 aaactccgcg cgcgaggcga ttgccggcag cgactgaact tcggttttag cttgttgtcc
     1981 ggcggcgctg atgctgcaaa gcagtagtgc gagcgcgagt aagggtgtcg ggtgacgggt
     2041 gactagtgtc agttgtctaa gttggaggga cgcgtgcacc tattaaccgc caagttgccc
     2101 agagcgcgaa gaataccaag aaggatagcg gaaaacttat ttagggatct gacccgtgaa
     2161 ccaaacgtta caacctcgtc acctgatccg gtttcatgca tccgctccgg tcacccgata
     2221 cctgacaccc gttaccctgc ttttaaggtt tcgcagcagg cagccgggtc cgcggtggtg
     2281 gcggtgtagc gcgggttgtc ggggctggtc cgcgcggcgc gttacgtccg ctgcgagtaa
     2341 gcgcaatgcg tttgtcacga ccgggggcat cggcgactgt aaccctgatt gcccgataag
     2401 aaccgtcgcg tgctttgtta gtgggattgt aggccagaac gtattgcgtt cgcaggtcgc
     2461 gtacgatctc gttggctatt tgtggcaact cggaaagcga ttgcggaaag aacgcccgcc
     2521 cgccggtctc ggacgccatt ttgttaatga gattgacgga gcgctcccgc gggctcttgc
     2581 gaataaagcc accttccttg tccagctcat tcacaaatcc gatcacataa atctgtacgt
     2641 cctgctcgcg cagacgcgca aacagttgct ccggtttgta gaagctgacg cgatcctcgc
     2701 catcagtcac aacaatcaag gcacggcggc ggcgatcgtt gtcatcacct ttcttgtatt
     2761 cggcgaggcg ctccgcggca aggtagacag cgtcaattac cgccgtttgc ccgccctcca
     2821 cataaagacc atcgagcgcg tcgatcagca aatcctgatt tgaagtgaaa tcctgctccg
     2881 taatgatctt gtcgctactg ataaagcgaa ccaggaatgt ctcgtctcct ggcctgttcg
     2941 tgttaatgat gctcttgccg gcatcgatca cgctttgtaa ctgcgaacga agtga
//
LOCUS       DASUEX010000304         4238 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_383395, whole genome shotgun sequence.
ACCESSION   DASUEX010000304 DASUEX010000000
VERSION     DASUEX010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4238
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383395"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="VFV61_05085"
     CDS             <1..252
                     /locus_tag="VFV61_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899885.1"
                     /translation="GPLGPGGGPLGPGGGPLGPVGSLDPGGGGGPGFGGGPLGPGGGP
                     LGPGGGPLGPGGGPLGPGGGPLGPGGGPLGPGGGPLGLP"
     gene            complement(725..3310)
                     /locus_tag="VFV61_05090"
     CDS             complement(725..3310)
                     /locus_tag="VFV61_05090"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX4899886.1"
                     /translation="MKIEPGTSLGRYKLRSLIGEGGMGQVYRATDITLNRDVAVKLLL
                     PDIAPGSEQFQRFEQEARAAGALNHPNILAIYDVGTQEGVSYVVSELLEGETLRERTR
                     SGPIPLKKALDYAQQIVRGLAAAHAKGIVHRDLKPENVFITADGHVKILDFGLAKLTN
                     PLGDFDGNSEAETVKLKTRAGAIMGTVGYMSPEQVKGQDVDHRSDIFSFGVMFYEMLT
                     GKRAFRGESPIEIMSAILNKEPAEPAQADRALAPPFERVVRHCLEKRPADRFQSTRDL
                     AFDLESLASAVTTSGSVLTMRPAVKSDRSKLIWPLAAIMLSLIALAAAYLLGKRSVSR
                     DTPSYHQLTFRRGTIYSARFSADADTVVFSATWNGNPLDIYEMRSGSADSKPLGLTNV
                     EVLAVSASSEMAVLLNSQQLYHSVRRGTLARMPLGGGAPREMLENVQEADWGPDGNLA
                     VVRFAQDRNHLEYPIGKVLYETDGYISNVRISPKGDAIAFLDHPVDGDDRGSVMLIDS
                     NGQKKKLSGDWAGEDGLAWSPSGKEIWFTATRSGEAQALYAVTISGEERVVARAPITL
                     RLQDISRDGQVLLTGESQSTPISCLVPGETKERELSWLNSVRINDLSRDGKTFVFTHF
                     GQSSGTNYHVYLRQTDGTSAIQLGDGYGGSFSPDGRWVSSILSSPPQILLLPAGAGQT
                     KRLESFGIEQFGYSTAWLPDGKSIVFNGKEPGRLLRTYVQATDGGPPRPVTPEGSTGS
                     LVSPDGQFLLARDLAEKEAIAIYPLNGGEPRPIPGLEEEDRVIRWGLDGKSLYVYRPR
                     ERPLKLYKLNLATGQKEVEKEIVPADLAGILGPINVLMTPDARGYIYAFTRSLSDLYL
                     ATDLH"
     gene            3493..4041
                     /locus_tag="VFV61_05095"
     CDS             3493..4041
                     /locus_tag="VFV61_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899887.1"
                     /translation="MKRLPLSLPLSLLFLVTIHSTPLVNQQETATPKQVETTYDEIKD
                     KTTVRLLPVPISSAKAHYHSVHIAPAFSYPGHKFKKPEIIDFEVQTVVKTRLKIDLYV
                     VFVVDGETIFLSSNRWAVKRPVPGKRWSGERLVFRMPYETLLKLTKAKTVDIKMDGVE
                     FPLTEPVLERLRMFAHVTEAAS"
BASE COUNT          917 a         1169 c         1183 g          969 t
ORIGIN      
        1 ggtccgctag gtcccggcgg tggtccgcta ggtccgggcg gtggtccgct aggtcccgtg
       61 ggttcgctcg atcccggtgg cggaggcggt ccaggtttcg gcggtggtcc gcttggtccg
      121 ggcggtggtc cgcttggtcc gggtggtggt ccgcttggcc cgggtggtgg tccgcttggt
      181 ccgggtggtg gcccgcttgg tccgggcggt ggcccgcttg gtccgggcgg cggtccgctt
      241 gggttgccgt aatcccctgc aaacgaattc gccgcaccta ccacgtagcc taatccgttg
      301 ctaacaacca gttcgtgggg cagggcaaat acaagggacg acaacaactc gtcgctaatc
      361 ggctcgccaa actcgcggaa cacctttgac actgccgcca aagattcaag ccgcaagcgg
      421 ttattcatct gaaatctaaa tcgaaggttt tcagcctcgc gcaaagactc aagttcttgc
      481 atgggaatgt gctccttatt tcaagaagag acaatcgaag gccggtagag cagtggcacg
      541 catttcgatt gatggttctg gcggtggcga accccacccg gtcaaatgct ttgctcagtt
      601 gagtgtggaa gcaacagctt cctactctaa ttccctccta ctgagctcgc ttggttggat
      661 tggggcggag tctatacgca aagttgattt ttttcaaacg aacgctggac cggggacaac
      721 gcccttaatg caggtcggtg gccagataaa ggtccgaaag agatcgcgta aacgcataga
      781 tgtatcccct ggcgtcgggt gtcatcaaga cgttaatagg gcctaggatg cccgcgagat
      841 cggcaggaac tatctctttc tcaacttctt tttgtcccgt cgcgaggttg agtttgtaga
      901 gcttcagcgg ccgttctctg ggacgataga cgtagagaga tttcccgtcg agtccccagc
      961 gaattactcg atcttcctcc tccaagccgg ggatcgggcg cggctcaccg ccgttcaagg
     1021 ggtaaattgc gatagcttct ttttcggcca ggtctcgagc caaaaggaat tgtccgtcag
     1081 gggaaacaag gctccctgta gatccctcgg gtgttacggg ccgcggagga ccgccgtcag
     1141 ttgcttgcac ataagtgcgt agcaaacgtc cgggctcttt gccattgaag acgatgctct
     1201 tgccatcggg taaccaggcc gtgctgtatc caaactgctc gataccaaag ctctcgagac
     1261 gtttggtttg tcccgcgccg gcaggcaaaa ggagtatctg cggcggcgaa gaaagaatgg
     1321 aagaaaccca cctgccatca ggcgaaaaac tgcccccata tccatcgccg agttggattg
     1381 cagatgtgcc atcagtttgg cgaagataga catgatagtt agtgccacta ctctggccaa
     1441 agtgagtgaa tacgaacgtt ttcccatcgc gagatagatc gttaattctc accgaattta
     1501 gccatgagag ctcacgctct ttagtttcgc cgggaactaa gcaggaaatc ggcgtcgact
     1561 gactttctcc agtcagcaac acctgcccat cacgagagat atcctgaagc ctcagggtta
     1621 ttggtgcacg ggcgaccacc ctttcctctc ccgagatcgt aacggcatac aatgcctgcg
     1681 cttctccgct tctcgtcgct gtaaaccaaa tctccttccc ggagggcgac catgcaaggc
     1741 cgtcctcccc agcccaatca cccgagagtt ttttcttttg accgtttgaa tcgataagca
     1801 ttacggagcc gcgatcatcg ccgtcaaccg ggtgatcgag aaaagctatc gcatcgccct
     1861 tgggagaaat gcgcacgtta ctgatatacc catccgtctc gtagagaact ttgccgattg
     1921 gatactcaag gtgattgcgg tcttgcgcga agcgaacgac cgcaaggttg ccatccggcc
     1981 cccaatcagc ttcctgaaca ttttccagca tttcgcgggg ggcgcctccg cccagtggca
     2041 tccgcgccag tgtgccgcgt ctgacagaat gatagagttg ttgactatta agaagtactg
     2101 ccatctcact gctagcagac acggccagca cttcaacatt cgtcagcccg agaggtttcg
     2161 agtccgcgga gccggagcgc atctcataga tgtctagtgg attgccgttc caggtggcgc
     2221 tgaaaaccac cgtatcggca tcagcactga agcgagccga gtagatcgtc ccgcgccgga
     2281 acgtcaattg gtgataagaa ggagtatccc ggctgacact gcgctttccg agaaggtatg
     2341 ccgcggcgag cgcaatcaga ctcaacatga tagccgccag cggccagatt aatttgctgc
     2401 gatcactttt caccgccgga cgcatggtga gtactgagcc agaggttgta accgcgctgg
     2461 ccaacgactc gagatcgaag gccaggtccc tcgttgactg gaaacgatcg gcaggccgct
     2521 tttcgaggca gtggcgcacc accctttcaa atggtggcgc gagtgcgcgg tctgcctggg
     2581 cgggttcggc tggttccttg ttcaggatcg cgctcatgat ctcgatcgga gactcgccgc
     2641 gaaacgcccg ctttcccgtc agcatctcgt agaacatcac tccaaaagaa aaaatgtcgg
     2701 agcggtgatc gacgtcctgg ccttttacct gctccggtga catataaccc accgtgccca
     2761 tgatggcccc ggcacgagtc ttcaatttca ctgtttcagc ttcggagttt ccgtcaaagt
     2821 cgccgagcgg attggtgagt ttggcgagac caaagtcgag aatttttacg tgcccgtcgg
     2881 cggtgatgaa tacattttcc ggcttcagat cccgatgcac aattcctttg gcgtgtgcgg
     2941 cagctaagcc gcgaacaatc tgctgcgcat aatcaagtgc ctttttaaga ggaatcggac
     3001 cactacgagt cctctcacgc aaggtttcgc cctccaacaa ttccgacact acatacgaca
     3061 cgccttcctg agttccgacg tcgtaaatcg caaggatatt gggatggttt agcgcacctg
     3121 cggcgcgcgc ctcttgctca aagcgctgga actgctccga accaggcgca atatccggaa
     3181 gcagaagttt gaccgcaaca tcccgattca gtgtgatatc ggtcgcgcgg tagacctgac
     3241 ccatgcctcc ttctccgatt agcgaacgga gcttgtacct gccgagactg gtgccgggtt
     3301 cgattttcac ttgtgctggt tgtaccgtat ggtgctggtt gggcctgggt tgaaaactga
     3361 cgtgccggat tctagtttac tttggcggca cgcgccagag gggacttcgc tgggacagta
     3421 tcacaatgaa gcccaagctg tgagcttgtt cacgcaaata atagtttgga ctgcttctca
     3481 cctgtgtgac ttatgaaacg gctgcccttg agtctgccac tctcgcttct tttcctcgtc
     3541 acaatccact ccactccgct tgtgaaccag caggaaacgg ccacgcccaa acaggtcgaa
     3601 actacgtacg atgaaataaa ggataagacg actgtgcggc tgctgcccgt gccgatttcc
     3661 agcgcaaaag cgcattacca cagtgttcac attgcacctg cgtttagtta tccgggacac
     3721 aagtttaaga agccggagat catcgatttc gaagtgcaga cggtggtgaa gacgaggctg
     3781 aaaatcgacc tttatgttgt gtttgttgtc gacggcgaaa cgatttttct gagctcaaat
     3841 cgctgggcgg tgaagcgtcc ggtgccgggc aagcgctgga gcggcgagcg tctcgtgttt
     3901 cgcatgcctt acgagacctt actaaaactg acaaaagcga agaccgttga catcaagatg
     3961 gatggggtcg aatttcctct caccgagccg gttttagaga ggctgcgaat gttcgctcac
     4021 gtgactgagg cggcaagtta ataacagtga catcagggcg gcgatcgaga cattgccctt
     4081 aatgtctcac gcgaatgaga tatccggctt agccgcccgg tgtgaggcgg tggctatgcc
     4141 ccgccgtgat cgcccaattt attccggggc tatgcccctt gttcgggctt tcgcccgtcg
     4201 actaataacc aaaaagcagc acagagggcg taggccaa
//
LOCUS       DASUEX010000305        11661 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1641874, whole genome shotgun sequence.
ACCESSION   DASUEX010000305 DASUEX010000000
VERSION     DASUEX010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 11661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11661
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1641874"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(190..1140)
                     /gene="ttcA"
                     /locus_tag="VFV61_05100"
     CDS             complement(190..1140)
                     /gene="ttcA"
                     /locus_tag="VFV61_05100"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF007972.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HEX4899888.1"
                     /translation="MRISVGLFRLNYCQSVCRRGILWHHQKGVNRREMAESKIKTKLL
                     RRMGETIEKFAMIQDGDRIMVCLSGGKDSYTLLDLLLDIQQRAPVNFDLLAVNLDQKQ
                     PGFPATVLPEYLAARSVPYRIIEEDTYSIVKRLVPEGKTFCAVCSRLRRGVLYNVAVS
                     EHCNKIALGHHADDIIATFLLNILYGGALKAMPPVLRSTDGRNTVIRPLAFCRESDIA
                     DFADAQQFPIIPCDLCGSQANLKRARVKRLLDELEKEIPHVRGSMLKALSNVKPSHLL
                     DAELFDFKMLTSARGDVAAELDLALGHTDTELTPALVSIM"
     gene            1417..2100
                     /locus_tag="VFV61_05105"
     CDS             1417..2100
                     /locus_tag="VFV61_05105"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEX4899889.1"
                     /translation="MLRIGKNPKDQNDSEHSSDRQEAPSYSTQQPYAPAASPAPRADF
                     TSTPAKAMTESENIAREIKEGTLSGFVGSGTELTGETTFKAMLRVDGHLSGKISSSSG
                     TLIVGATGKIDANVEVAVAVVHGTVNGDIIASQRLELGRASKVNGNIQTPSLIIEQGG
                     IFEGNCKMLKMTAALDKSRDEKRVQDTKAPLDTTGMKTTPADVPKPAEMPNYAAKSAE
                     ASPAAKLAN"
     gene            2452..4599
                     /locus_tag="VFV61_05110"
     CDS             2452..4599
                     /locus_tag="VFV61_05110"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX4899890.1"
                     /translation="MFFAAFGAIAGSVHGQSRQTPVVPKTALLQIIRAEDERRWDGVL
                     SGLMSHANPAVRGRAALAAGRIGNELAVLALISLMQNDKDRRVRSLAAFALGEVESPK
                     AVAALTSELSKGDAPRARIVEALGKIAAALPQTSEAQKKEIAKTILDILTFEARRRSR
                     PDSEVILLALTATLRARPEGAGPVVAQFLSYSDPRIRSDAGNTLARLRAADGNAELRK
                     LLTSDPDPNVRANAARVLGATEDKAAYDGLLDRVWNDPDKRVRISAIRAVAALKDQRA
                     ADRLLKIQLAGDDELLELATAIGRLLAGTENRAAIAWLKNTRARLSRAAEVETAFARI
                     APVAYLDELGSAEAGKRKVQETMLLDWRAAASIAGALGEIAALPDSAKDKVMLAKTAQ
                     ELLRAMLDYRNSGIKINTLVAVHSEYAIPDVLRALSAFKPTSADSLYRRQLGESDVVV
                     RATAAELLGDLPISSENIAALSLALKAALQEKESNDAALAIIDTLAKYKDRSANEAMM
                     TALDSNDYIVRRKAIEALKTSGGGDFSNRLGYAKTRTSASDYERAISRIGKRVLATVN
                     TNKGRFTIELLPAEAPLNVDNFVQLAKRRYFNGIVFHRVVPNFVIQGGDPRGDGNGGP
                     GYQVRCEINEVPYQRGAVGMALSGKDTGGSQWFVTHAPQPHLDGGYTVFGNVVAGMNV
                     VDNIVRGDRIISIVVTEKRGPKMQSSPITVLEN"
     gene            4716..6419
                     /locus_tag="VFV61_05115"
     CDS             4716..6419
                     /locus_tag="VFV61_05115"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HEX4899891.1"
                     /translation="MIATTSFAGITTAQTREAVPGERSRYQIELKIDVDALSYSGSER
                     VRWINRGDHATSTLYFHLYSNLRVEGQVASVADEPQIEVTKVRLAATDAPLPFALEEQ
                     GTTLRVNLRESVPVNGAVEVALDFRGNVPEVDPEESAITTHVIKQVSAALRDEREMRR
                     ARDLNFRCRGVMLLGSAYPVLAVHDGDEWRRRLEPGIGDIIFNESADYEVTVSSAPGV
                     NVFASGTEEARDGQEIKTFSGFALRDFAIVAGRGLRSEQTTVGQTTLRSVFLAEHERV
                     GKHVLSSSAAALRVFSARFGSLPYKTITVAEAPLVAGLGSTEFSGLLVIASAFYVDFD
                     SPAMRNLPEIIREQRPAVEQSLEWTVAHLLAHQWWGTAVGNDPAREPVLDEALACWSA
                     LLYYEAVHGKEQAAIVQEDQLLGVYRLYRTFGGEDMDANRASRDYRNSFQYAAIVATK
                     GAMMFVVLRNLLGDEKFFAALSSYYKANQFEIARLEDLQGAFIAESPLEQRRTVARTF
                     NRWLESKRGDEDIAKPDQELAASLGIPSKPNRQKKGDRTALSAFAKLGKFFWQQMTRI
                     R"
     gene            6444..6998
                     /locus_tag="VFV61_05120"
     CDS             6444..6998
                     /locus_tag="VFV61_05120"
                     /inference="COORDINATES: protein motif:HMM:NF024822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4112 domain-containing protein"
                     /protein_id="HEX4899892.1"
                     /translation="MSKVLRAYKEDDLNLDRRSAGRPIRAIEIEESLDRLGWVLDDLF
                     RIPVLGWRIGLDAIVGLIPGVGDTATTVASLYVLGSAVRYRVPKVTLLRMGLNLGIDY
                     VLGSLPLVGDLFDAWWKSNQKNVALLRQRATVSASEAREGRISDWLFVGMIMVLLVLL
                     AIGAALISFYLLAMLFQSLASFAS"
     gene            complement(7006..7503)
                     /locus_tag="VFV61_05125"
     CDS             complement(7006..7503)
                     /locus_tag="VFV61_05125"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEX4899893.1"
                     /translation="MAMRIGTEMPKLDGATEWLGTTQAHAEAEAVGHPTIVHFWSVSC
                     GICKENLPRIAEWRDSGKEQGLRVIAIHMPRYEADTDVEAVRDAIATYKISEPCAVDN
                     EHKLRDAFKNDQGYVPAYYLFDAEGKLRGFAAGERGLDLLKATLDRVMRQSAPAETAE
                     LISTS"
     gene            complement(7582..8127)
                     /locus_tag="VFV61_05130"
     CDS             complement(7582..8127)
                     /locus_tag="VFV61_05130"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019258276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX4899894.1"
                     /translation="MEKAMVGQPAPDFDMASTKNLEKLNENVKLADYKGRWLVLLFYP
                     LDFTFVCPTELTAFSDRYEDFQGIGADIIGVSTDSAFSHRAWINTPRDKGGVEGLKYP
                     LAADATKEVARDYGVLIENRGIALRGLFVIDPEGILRYKVVHDLNIGRSAEETLRVIQ
                     ALQTGGLCQAEWKPGQKTLNA"
     gene            complement(8194..8616)
                     /locus_tag="VFV61_05135"
     CDS             complement(8194..8616)
                     /locus_tag="VFV61_05135"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEX4899895.1"
                     /translation="MQKDSTGRDKQRLTPQREAVLRAVRERDDHPTAGDIFAAAKQYL
                     PAISYATVYNSLRYLRQAGLVRELSFGDGASRYDGITDRHDHALCTECGKLVDFDLAE
                     PAELMQEAARKSRFQAESVHLTLVGRCPDCLEIKANDS"
     gene            complement(8734..9822)
                     /locus_tag="VFV61_05140"
     CDS             complement(8734..9822)
                     /locus_tag="VFV61_05140"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX4899896.1"
                     /translation="MTAGETIFVTGFPGFIARRLVQRLAQTGGKFLLLVQPALASQAQ
                     ADLQEIAGQAQKPLQDFQLLEGDISQPDLGLSPFELQTALSEVTLIFHLAALYDLAVA
                     REPATLVNVTGTRNVNTLAQSARNLRHYHYVSTCYVAGKRRGRIRETDLKHDAGFRNY
                     YEETKYLAETEVAGLKADLPITIHRPSVVCGDSVSGETAKFDGIYYLINYLLKSPALF
                     SILNIGNREVKLNLVPVDFVVESLAALAQDSRAVGKTLQLADPNPLSTRELFDVIAEE
                     ITGHGSRMSLPPKLVHFSLMLPFSPAITGLPHSAVPYFFLDQTYDTTEATALLSPLGI
                     TCPAFPAYADRIIAFAARDRANRRIAPL"
     gene            9989..11545
                     /locus_tag="VFV61_05145"
     CDS             9989..11545
                     /locus_tag="VFV61_05145"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX4899897.1"
                     /translation="MKSNRHVVRNTARLALLLVTCVAAIHAQEEECTTRITLLQVNDV
                     YQFAPVDRGTRGGIGRVITLKKQIQKESPHTLFLFAGDTISPSVESITYKGAQMIEAW
                     NVAGLDYATFGNHEFDFGPEVLLERIKESRFGWLATNVVDKKTGQTFGGVPPYVIREF
                     DGVKIGIIGLVLPETKTTSRPGTDVEFFDPCETANEVIPEIHLKGAQVVVALTHLSMR
                     EDKEVARCADVDVIIGGHEHTLLESAAGGAPIFKMTSDARELGRIDLNISRTSGKLES
                     IDWKVIPVTAEIKEDADLAPVYSKYAGLLRDLSQVVGRTTVALDARSAESRTRETNVG
                     NLITDAFRKATRADVAVMNGGSIRADALVSAGSLTKRDLLAIIPFKNKVVKLELTGAA
                     LRTVLEHGVARSAEDSQPGRFPQVSGIRFTFDAGRPAGSRVVDVSVNGVPLDDQKKYS
                     LATTNFLAEGGDGYDILKTARVLIASAQGQSDFDVVRQSLAVGPITPKVDGRIKRLDN
                     ARRKQVECPE"
BASE COUNT         2660 a         3045 c         3361 g         2595 t
ORIGIN      
        1 tgctgctcat caggtgggag cggatggcgg aataaacctt cggttgatcc ggctctctga
       61 ttcacaccgg gcttgagccc ggtgacaagc aatagtcatt aagatgggaa accgtttaaa
      121 cggtttcagg ttttcgtcac acaggaacac cgcgcttaag cgcggtgaga atgagaacga
      181 cccgcttgct cacatgatcg acaccagtgc cggtgttagt tccgtatctg tatgacccaa
      241 agccaaatcg agttcagccg ctacgtcgcc ccttgccgag gtcagcatct taaaatcgaa
      301 aagttcagca tcaagcaaat gcgaaggctt tacattactc agcgccttga gcattgaacc
      361 gcgcacgtga ggtatttctt tttcgagctc gtcgagcagc cgtttgacgc gcgcgcgttt
      421 taagtttgct tgcgaaccac aaaggtcgca gggaatgatg gggaactgct gtgcgtccgc
      481 aaagtccgct atgtccgatt cgcggcagaa agcgaggggc cgaatcacgg tgttccggcc
      541 atcggtggac cgcagcaccg gtggcattgc tttcagcgcc ccgccgtaga ggatattgag
      601 gagaaaggtc gcgatgatgt cgtcggcatg atgaccaagg gcgatcttgt tgcagtgttc
      661 gctgacggcg acgttgtaaa gaacaccccg ccgcaggcgt gagcagacag cgcagaaggt
      721 tttaccttcc ggaaccagac gcttgacgat cgaatatgtg tcttcctcga tgatgcggta
      781 gggaacggag cgggcggcca gatattccgg tagcaccgta gccggaaacc cgggttgttt
      841 ttgatccagg ttgacagcca ggaggtcaaa gttaactgga gcgcgctgct gaatgtccag
      901 tagtaagtcg agcaaggtgt agctgtcttt gccgccagag agacaaacca tgattcggtc
      961 gccgtcctga atcatcgcaa acttctcgat cgtctcgccc atacggcgca gtagtttcgt
     1021 tttgatcttg ctctcggcca tttctcgtcg atttactccc ttttggtgat gccagaggat
     1081 gccacggcgg cacacactct gacaataatt gagcctaaag agcccaactg aaattcgcat
     1141 gatcgtggcg agaagggcac ggcgccccgg cgaatgctca cttccgtgtg tctgcaaaaa
     1201 caattccgat tacattttca ttacagggct agacacgtga gttcgagtgt ggctattatg
     1261 agttcccgct ttgaggcggt cgacgatcac acggctagcc tccagctagc gaacaatcca
     1321 aaacaggaag gcaagcgcgt cgttttgttg cttctaccta acccgattgg cactgtgact
     1381 aaacttaccg cggcccccat tcaacggagg taataaatgc tcaggatagg aaaaaacccc
     1441 aaggatcaga acgacagcga acattcaagc gaccggcagg aagcgccctc ttattcgact
     1501 caacagcctt atgctcccgc agccagccca gcaccgcgtg cagatttcac gtccactcct
     1561 gccaaagcga tgaccgagag tgaaaacatt gcccgcgaga ttaaagaagg taccctgagt
     1621 ggttttgtcg gcagcggaac ggagctcacg ggtgagacta cgtttaaggc gatgctgcga
     1681 gttgatggtc acctctcagg aaagatcagc tcgagcagcg gaacactgat cgtgggtgcc
     1741 accggtaaga tcgacgcaaa cgtcgaagtt gcggtagccg tcgttcatgg gacagtcaat
     1801 ggcgacatca ttgcgtcaca gcgtttggaa ttgggtcggg cctcaaaagt gaatggaaac
     1861 attcaaacgc catcgttgat catcgaacag ggcggcatct ttgaaggtaa ctgcaagatg
     1921 ctcaagatga ctgccgcgct cgataagtcg cgcgacgaga agcgcgtaca ggatacgaag
     1981 gcaccgctcg ataccaccgg tatgaagacg acacccgctg atgtccccaa gccggctgag
     2041 atgcccaact acgcggcgaa gtccgcagaa gcttctccag ctgcaaaact cgctaactaa
     2101 cgcgggacca acacaagaac aaaacaggcg ctggctcgaa agagctggcg ccttttgttt
     2161 tgggatcatg atgttcgatt ggcgattgcc aattgccaat tgccaattag accgtagcga
     2221 aagaagccaa cggaaatccg caatcggcaa tcgccaattg gcattcgtca attggcaatt
     2281 cctaatcgct tgacatggat acagcacctc gttgatacat agaggtgtta atcccccttc
     2341 aagcaataca ttcaatagcg actcagccaa cacgagctgc gtaggcaaac ataaatgaca
     2401 ttgtctcgcc atctccgaac tcgacaacgg accagacttg tagctcttct cttgtttttc
     2461 gcagcctttg gcgcaattgc tggctcggtc cacgggcagt caagacaaac gccggttgtt
     2521 ccgaaaacgg cgttgctgca gattattcgc gccgaagatg aacggcgttg ggacggcgtt
     2581 ctctcaggcc tcatgtctca cgctaatccc gcggtgcgcg gtcgtgccgc cctcgcagcc
     2641 gggagaatcg gcaacgaact tgcggtcctg gcgctgattt cattaatgca aaacgataag
     2701 gatcgacgtg tgcgctcgct cgctgccttc gctttaggtg aagttgaatc acccaaagcc
     2761 gttgcggcgt taacgtcgga actgtcaaag ggtgatgctc cacgcgctcg catcgttgag
     2821 gcactgggaa agatcgcggc cgcgcttccg caaacgagcg aagcgcagaa gaaggagatt
     2881 gccaagacaa tcctggacat tcttacgttc gaagcccgcc ggcgttcacg gcctgactcc
     2941 gaagttattc tgctggcact gacggcgacg ctgcgagcgc gaccggaagg tgctggtccg
     3001 gtcgtcgctc agtttctttc ttactccgat ccccgcattc gctctgatgc gggcaatacc
     3061 ctggcgcggc tgcgcgccgc agatggcaat gcggagttgc ggaagttgct gacgagtgat
     3121 ccggatccaa acgtgcgcgc aaatgccgca cgtgtgttgg gtgcgactga ggacaaggcg
     3181 gcgtacgatg gcttactcga tcgcgtttgg aatgatcctg acaaacgggt tcgcatcagt
     3241 gccatccgcg cagtggcagc attgaaagat caacgagctg ccgatcgctt gttgaagatt
     3301 caactcgctg gcgacgacga actcttggaa ttggcaaccg caattggtcg gctacttgcc
     3361 ggaactgaaa atcgagcggc aattgcttgg ttgaagaaca cacgagcccg actgagtcga
     3421 gcggcggaag ttgaaacggc attcgctcgc atcgccccag tggcgtatct ggacgagctt
     3481 ggcagtgcag aggcaggtaa gcgaaaggtt caggagacga tgttgctgga ctggcgtgcc
     3541 gcagcaagca tagccggggc gttgggtgag attgcagcac tgccggattc ggcaaaagac
     3601 aaagtaatgc ttgccaagac agcccaggag ctgttgcgag ccatgcttga ttaccgaaac
     3661 tctgggatca agattaacac cctggtcgca gttcattctg aatatgcgat acccgatgtg
     3721 cttcgtgcgt tgtcagcctt caagccgacg agtgccgatt ctctttaccg ccggcagctg
     3781 ggggaatccg acgtggttgt tcgtgcaaca gctgcggaac ttctgggtga ccttccgatt
     3841 agcagcgaga acattgctgc tttgagtttg gcgttgaagg ccgccttaca agaaaaggag
     3901 tcgaacgatg ctgcgctggc aattattgat actctcgcaa agtataagga tcgttctgct
     3961 aatgaggcga tgatgacggc gctggactcg aatgactaca tcgtgcgccg gaaagcgatt
     4021 gaagcgttga aaacgagtgg gggcggagat ttcagtaacc gattggggta tgcgaaaacg
     4081 agaacctccg cgagcgatta tgaacgagct atctcccgga tcgggaagcg cgtgctggcc
     4141 actgttaata cgaacaaagg acgtttcacc atcgagctgc tgccggcaga ggcgccgctc
     4201 aatgtcgaca attttgtgca actcgcgaag cgccgttact ttaacgggat cgtgtttcat
     4261 cgggtggtgc ctaacttcgt aatccagggc ggcgatccgc gcggagatgg taacggcggt
     4321 cctggttacc aagttcgttg tgagatcaat gaagttccct accagcgcgg cgcagtaggt
     4381 atggcgttat ctggcaaaga cacgggcgga tcccaatggt tcgtaacgca cgctccgcaa
     4441 ccgcatctcg acggcggcta cacggtcttt gggaatgttg ttgccggcat gaatgtcgtc
     4501 gacaatattg ttcgtggcga ccgaattatt tcaattgtcg tgaccgagaa acgaggtccc
     4561 aaaatgcagt cgtctcccat cactgttctc gaaaattagc gtgccttagt tgagaagttt
     4621 tggttaaaag atccaatcaa ccagttgagt cagcacgaat ctgcggcaaa gtttcgcgca
     4681 gggttagcga gcgcctgtgt tgcggccttt tgctcgtgat agcgaccacg tcgttcgccg
     4741 gcataacaac cgcgcaaacg cgggaagcag ttcccggcga gcgtagtcgc tatcagatcg
     4801 agttaaagat cgacgtcgac gcgctctcat attcaggttc cgaacgagtc cgctggataa
     4861 accgcggcga tcacgctact agcacactct attttcatct ctattccaac ctgcgcgtcg
     4921 aaggccaggt ggcttcagtt gccgatgaac cgcaaatcga ggttacgaag gttcgccttg
     4981 cggcgacgga tgcgccgttg ccgttcgcgc ttgaagagca gggaacaacc ttgcgagtca
     5041 atctgcgtga gtcagtgccg gttaatgggg cagtagaggt ggcgttggat ttccgcggca
     5101 atgtgccgga agtcgatcct gaagagtcgg ccatcactac gcacgtcatc aagcaagtca
     5161 gtgccgccct tcgtgatgag cgcgaaatgc gccgcgcccg cgatctgaac tttcgctgtc
     5221 gcggcgtgat gctgttgggt tccgcgtatc cggttttggc ggtacacgac ggtgacgagt
     5281 ggcgccggcg gctggaacct gggatcgggg acattatctt caatgagtca gcagattatg
     5341 aagtgacagt ttcctcggca ccgggcgtta atgtctttgc ttcgggaaca gaggaagcga
     5401 gagacggcca ggagataaag acgttttccg gattcgcctt aagagatttt gccatcgtgg
     5461 ccggtcgcgg cctccgctcc gaacaaacaa ctgtaggtca aacaactttg cgctcggtgt
     5521 ttcttgccga gcatgagcga gttggcaagc acgttctttc cagcagtgcc gccgccctgc
     5581 gagttttttc cgcccgcttt ggttcgttgc cctacaagac gatcactgtc gccgaagcgc
     5641 ccctggttgc aggattagga agcacggagt tttccgggct gctggtgatc gcgagcgcgt
     5701 tttacgtgga cttcgactct cctgcgatgc gcaatctgcc tgaaatcatt cgcgagcaac
     5761 gaccggcggt cgaacaaagt ctggaatgga ctgtcgcgca tctgctggcg catcagtggt
     5821 ggggaacagc agtcggaaac gatcccgcac gcgagccggt gctggacgaa gcgctcgcct
     5881 gctggtcggc gctgctttac tacgaagccg ttcatggaaa agagcaggcg gcaatcgtgc
     5941 aggaggatca gttgctgggg gtttaccggc tgtatcgcac cttcggtggg gaagacatgg
     6001 atgcgaatcg tgcttctcga gattatcgca actcctttca gtatgcagcc attgttgcca
     6061 ccaagggcgc gatgatgttt gtggtgctgc ggaatctgct cggggacgag aagttttttg
     6121 ctgcgctcag cagctattac aaggcaaacc agtttgagat cgcccggctg gaagatctcc
     6181 agggagcgtt cattgccgaa agtcctcttg aacaaagaag aactgtggct cgtacgttca
     6241 atcgctggct ggagagcaag cgcggtgacg aagacatcgc caagcctgat caggaacttg
     6301 cagcctcgtt aggaattcct tccaagccta accggcaaaa gaagggcgat cgcactgcgc
     6361 tgtcagcttt cgcaaaactg ggaaagtttt tttggcagca gatgacacgc atacgttaag
     6421 gagttaaggg aacactaaga actatgagca aggtgttaag agcttacaaa gaagacgatc
     6481 tgaacctgga cagacgcagc gccggcagac caattcgtgc tatcgagatc gaagagagtc
     6541 tggatcgcct gggttgggtg ctggacgatc tctttcgtat tcccgtgctc ggttggcgca
     6601 tcggtttgga tgcgatcgtc ggtttgattc ccggcgtagg ggacactgcg acgacagtcg
     6661 cgtctttgta cgtgctcggt tcggccgtgc gctatcgcgt accgaaagtc acacttttgc
     6721 ggatgggtct gaacctgggg atcgactacg tgttgggctc gctgccgctt gtgggagatc
     6781 tcttcgacgc gtggtggaag tcgaatcaga agaatgtagc tctcttgcgg cagcgagcaa
     6841 ctgtttctgc aagtgaagcc cgcgaaggcc ggatcagcga ctggctattc gtcggaatga
     6901 tcatggtatt gctggtgctc ctcgcgattg gcgcagcgct gatcagcttt tatctgctcg
     6961 cgatgctgtt ccagagcctg gcttcatttg ccagttagaa tgttcctacg aagttgaaat
     7021 cagctcggct gtttccgccg gcgctgattg gcgcatgacg cgatcaaggg tggctttcaa
     7081 cagatccagc ccgcgttcac ctgcggcgaa acctcgcagc tttccttcag catcaaatag
     7141 gtaataggcc ggcacatagc cctggtcatt cttaaacgcg tcccgcaatt tgtgttcgtt
     7201 gtccaccgcg catggttcgc tgattttgta agtggctatg gcatcgcgca cggcttccac
     7261 gtccgtgtct gcttcatagc gaggcatgtg aatggcgata acgcgcaagc cctgctcctt
     7321 gcccgaatcg cgccattcgg caatgcgtgg caaattctct ttgcagattc cacaactgac
     7381 tgaccagaaa tgaacgatcg ttggatgacc caccgcttca gcttccgcat gcgcctgggt
     7441 tgtgccgagc cactccgttg ccccgtcgag tttgggcatc tcggttccga ttctcattgc
     7501 cattgttgca ctcctaaatt cgtgatgggc gataggaaga aacggtctca cctatcaccc
     7561 atcagcgatc tcactggtta attaagcgtt gagcgttttc tgacctggct tccattcagc
     7621 ctggcacagt ccaccagttt gcaaagcctg gataacgcgc agcgtctcct cggctgaacg
     7681 accgatgttc aagtcgtgaa ccactttgta acgcaggatc ccttccggat cgatcacaaa
     7741 caatccgcgc agagcgattc cccggttctc aatcaacacg ccgtaatcgc gcgccacttc
     7801 tttggtagca tccgcggcca gcgggtattt aagaccttcc acaccgcctt tgtcgcgcgg
     7861 tgtgttgatc caggcgcgat gcgaaaaagc agagtcggtg gaaacaccga tgatatcagc
     7921 gccgataccc tgaaagtctt cgtagcgatc gctgaacgcc gtcagttccg taggacaaac
     7981 aaaagtgaag tcgagcggat agaaaagcag tacgagccag cgtcccttgt agtcggccaa
     8041 tttcacattc tcgtttaact tttccaggtt cttcgtcgag gccatatcaa aatccggcgc
     8101 gggctggccc accatcgcct tctccattgc tactgctgtt gctgtcatgc atcctccata
     8161 agttttctag ttaagataat tccggttacc gtgttaagaa tcgttcgcct tgatttcaag
     8221 acaatcagga cagcgaccga cgagagtcag gtgaaccgac tcagcttgaa agcgagactt
     8281 acgggcagcc tcctgcatca gctccgccgg ctccgccagg tcgaagtcca ccaacttgcc
     8341 gcactcggtg cagagggcgt gatcgtgacg atcggtaatt ccgtcgtagc ggctggcgcc
     8401 atcgccgaag ctgagctctc gcaccaggcc agcctgacgc agatagcgca gagagttgta
     8461 gacggtggcg taactgattg cgggaaggta ctgcttcgct gctgcaaaga tatccccggc
     8521 agtgggatgg tcatcgcgct cccgcactgc acgcaaaacc gcctcgcgtt gcggcgtcag
     8581 ccgctgcttg tcccgccctg ttgaatcctt ctgcatagag tagagttata ctaattctaa
     8641 atcaatactc tgtcaactgg ctgagaaaag tcagggtttt gggtcttata tgcttacttg
     8701 tttccctcgc ggctagaaag ggccgggcca aactcacagc ggagctatgc gtctattcgc
     8761 cctatctctc gcggcaaagg caatgattct atcggcgtag gcgggaaagg ccgggcaagt
     8821 aattccgagg gggctgagca gggctgtcgc ctcggtggtg tcgtaggtct gatcaaggaa
     8881 gaagtaggga actgcgctgt gcggcagccc ggtgatcgcc ggggaaaacg gcagcataag
     8941 agagaagtgt acaagtttgg gcggcagact cattcgcgag ccgtgacctg taatctcctc
     9001 cgcaatcacg tcgaagagct cgcgcgtcga gagcggattg ggatcggcga gttgcagtgt
     9061 ctttccgacc gcgcgcgaat cttgcgccag agctgcaagg ctttccacga caaaatcaac
     9121 aggcaccaag ttcaacttca cttcgcggtt gccgatgttc aggatcgaga agagggcagg
     9181 cgacttcagt agataattaa tcagatagta gatgccatcg aactttgcgg tttcacccga
     9241 aactgaatcg ccgcaaacta cggatggccg atgaatggtg atgggcagat ctgctttcag
     9301 cccggcgact tccgtttccg caagatactt tgtctcttcg taatagttac gaaatccggc
     9361 atcgtgtttt aagtcagtct cacggatccg gccccggcgt ttgcccgcaa cataacaagt
     9421 cgagacgtaa tggtagtggc gcaggttgcg cgcggattgg gcaagcgtgt tcacgttgcg
     9481 tgtgccggta acgttcacga gcgttgccgg ttcacgcgcg accgccagat cataaagtgc
     9541 cgcgaggtga aatatcagcg tgacttcgga aagtgcggtt tgcagttcaa aaggactcaa
     9601 accaagatca ggctggctaa tgtcaccttc gaggagttga aagtcctgaa gaggtttttg
     9661 agcctgtccg gcgatctcct gcaagtctgc ctgcgcctga ctggcgaggg caggttgaac
     9721 cagcagtagg aattttccgc ctgtctgggc gaggcgttgc accagccgcc tcgcaatgaa
     9781 gccaggaaac cccgtaacga aaattgtttc gcctgccgtc atctaacttt gctgcaaaag
     9841 tcctgagctc gcaccgggtt cgcctaagcc gtaaactaag ttttgagcaa cgcttccgtc
     9901 atatcatcta aactgtcgtg aggctgaatt atactcggac aaaagtcgca gccggcaacc
     9961 ggcgctacat ttacagttgg gagacgagat gaaatcaaac aggcatgtcg tacgtaacac
    10021 ggcgaggctg gcgctgctcc tggtgacctg cgtcgctgcg atccatgcgc aagaggaaga
    10081 gtgcacgacc cgcatcaccc tgcttcaggt aaatgacgtt taccaatttg cgccggttga
    10141 ccgcggcacg cgtggtggca ttgggcgggt gataactctc aaaaaacaga ttcagaaaga
    10201 gtcgccgcat acgctcttcc tttttgctgg cgacacgatt tcgccctcgg tcgaatcgat
    10261 aacttacaag ggggcacaga tgatcgaagc ctggaatgtc gccgggctcg attacgccac
    10321 ttttggcaat catgaattcg atttcggtcc tgaggtcttg ctggagcgga tcaaggaatc
    10381 gcgctttggt tggctcgcga cgaatgtggt cgacaagaaa acagggcaga cgttcggcgg
    10441 cgtgccgccc tatgtgattc gagaatttga tggtgtgaaa attgggatta tcggcctggt
    10501 tctccccgaa acgaaaacaa cgtcccggcc cggaactgac gttgagtttt tcgacccctg
    10561 cgagaccgca aacgaagtaa tcccggaaat acacctaaaa ggagcgcagg tggtagttgc
    10621 attgacgcat ctctcaatgc gcgaggacaa agaagtggca cgttgtgctg atgtggatgt
    10681 gattatcggc ggtcacgagc atacgttgct ggaatcagca gcaggcggcg cgccgatttt
    10741 caagatgacc tcggatgcgc gcgaattggg aaggattgat ttgaatattt cgcgaaccag
    10801 cggcaagttg gagagcattg attggaaggt gattcccgtt accgcggaaa taaaagagga
    10861 cgcggatctc gctcctgttt acagtaagta tgcgggcttg ttacgcgacc tatctcaagt
    10921 tgtcgggcgg acgaccgtcg cgctcgacgc gcgcagtgcg gaaagccgca cgcgtgagac
    10981 caatgtgggc aatctcataa ccgacgcctt tcgcaaagcg acgcgcgccg acgtagcagt
    11041 catgaacggc gggtcaattc gtgccgacgc attggtgagc gccggttccc tgacgaagcg
    11101 agatctactg gcgatcattc ccttcaaaaa caaggttgtg aagctggaac taaccggggc
    11161 ggcgcttcga acagtgcttg aacacggcgt agctcgcagc gcggaagact cacagcctgg
    11221 ccgttttcct caagtttccg gaattcgctt tacgtttgac gccgggcgac ccgcaggttc
    11281 acgagttgta gacgttagtg tcaacggagt tcccctcgac gatcagaaaa agtacagcct
    11341 ggctacgaca aacttcctgg cggagggggg cgacggctac gatattctaa agacggcgcg
    11401 cgttttgatc gcgtcggcgc agggccagag cgactttgac gtggtgcgtc aatcgctggc
    11461 cgtcggtccg atcacgccga aggtagacgg tcgcatcaaa cggttggata atgcgcgcag
    11521 aaagcaggtg gaatgtccgg agtaagctga tgattgctga cgatatttac gaagacgata
    11581 cttcgccgtt ggcaaagtac gaagcgaagg aattatttca ggaggcttac gaagcgcagt
    11641 tggcgcagaa ctttgaaact g
//
LOCUS       DASUEX010000306         3251 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1553034, whole genome shotgun sequence.
ACCESSION   DASUEX010000306 DASUEX010000000
VERSION     DASUEX010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3251
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1553034"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="VFV61_05150"
     CDS             <1..361
                     /locus_tag="VFV61_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899898.1"
                     /translation="ERGLWGSIGRSRDLGLHGRKPVFRNLRQLVWSYFDPYVDFSGRI
                     KGYGLTNLYELGRYDWRFSPKNMHKIEDHLRAIPHRSLKSSNERYRRLHERYKAFKLE
                     HPDEQPPYYDRQDQWML"
     gene            383..931
                     /locus_tag="VFV61_05155"
     CDS             383..931
                     /locus_tag="VFV61_05155"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HEX4899899.1"
                     /translation="MSRPGSKRKTAIAPKAPLTPEERRQQTFQRAAKLLSAKSRSVAE
                     LRELLLQRQGASKAHVEEVLARLTEYGYLNDERFALSYASAKVRQRPIGRQRLKRNLA
                     LKKVSKETADEALDLVFAETSESELIDRAIEKRVRLRGRPGTRAAAKSLFDHLLRQGF
                     PFELVAEKVRAAAQANIDDDEL"
     gene            complement(1159..3135)
                     /locus_tag="VFV61_05160"
     CDS             complement(1159..3135)
                     /locus_tag="VFV61_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899900.1"
                     /translation="MRTRNLSLLFLWLLGMSISASAQEQNPGPKQGDTVTEAGVSSEA
                     AAETLVRAAYAKLTRYNRAYLLVHDPRRENAPLDESYLRFELSNFRVGAIQEILNLPH
                     DEVLPAGTEILQLGRSVQTLNDADPHVAYWARWTTERYAALYNPKWTVNDLLSYDPAR
                     YHDVGFYVKYDVTLRFHGKVRIYSAVTLFRNPFGSVKSLKPIFWDTVVSSAGDFESLW
                     YEQRPAVGEPGQTNEEAQPHKSVNLQRLPPPISASSGVLAHTLLIKAGGTTPLAATQT
                     SSDTPSLGPVVENTTEDYREHSSGAHGERISFQGACEAPTTTQQTCRVNFYGIYVYEN
                     GSVTNLVYSHRNRYDESKGTASGPRGTPISCYSGYGVATKNCIDPNCNFTATLVGEGL
                     SMQMTGGDVWRGQLVHGHTCNIPKPFGGGGGGGGGCNMAPLKTAKSAMRIPAPNLINP
                     YCCDAVEQSTCFNGGGEWSDSTCSCYSPIVIDVAGNGFDLTNATNGVMFDLNATGIAE
                     QISWTAAGSDDAWLVLDRNGNGVIDNGRELFGSSSPQPYLVPGEMKNGFRALSMFDDP
                     DNGGNGDGQIDGNDSVFSSLRLWQDGNHNGISESGELQSLLDSDVRVIELKYRDSRRK
                     DDNGNWFRYRAKVNDSQGGQVGRWAWDVFLQKPH"
BASE COUNT          795 a          856 c          843 g          757 t
ORIGIN      
        1 ggagcgggga ttgtggggat cgattggacg ttcgcgtgac ttaggactac atggacgcaa
       61 gcccgtattt cgcaacttac ggcaactggt ttggagttac ttcgatcctt atgtggactt
      121 tagcggacgc atcaaaggtt atggcttgac caatctttac gagctaggca ggtacgattg
      181 gcgtttctcg cctaagaaca tgcacaagat tgaggatcac ttgcgagcga ttccgcaccg
      241 ttccttgaaa tcatccaatg aacgctatcg caggcttcac gagcgctaca aggcgttcaa
      301 gctggagcat cccgacgagc aaccgccgta ttatgatagg caagatcaat ggatgctgta
      361 gagaatctgg cagctgccga acatgtcgcg tcctggaagc aaacgtaaaa cagcaatagc
      421 gcctaaggct ccgttaacgc ctgaggaacg gcgccagcag acttttcaac gggcagctaa
      481 gttgctgtct gcaaaatcgc gttcggtagc cgaactgcgc gaactgctgc tgcagcgaca
      541 aggcgcaagc aaggcacacg tcgaagaagt gctggcgcgt ttgacggaat acggttatct
      601 gaatgatgag cggtttgcgc ttagttatgc ctcggccaaa gtgcgccaaa gaccaatcgg
      661 ccggcagcga ttgaaacgca atttggcttt gaagaaagtg agcaaagaaa ccgcggatga
      721 agctttggat ctggtgtttg cggaaacctc cgagagcgag ttaattgatc gcgcaattga
      781 aaagcgtgtg cgactgcgcg gtcgcccggg tactcgcgcc gcagcgaaga gtctcttcga
      841 tcacttattg cggcagggtt ttccgtttga actggtagca gagaaggtgc gagcagctgc
      901 ccaagcgaac atcgatgacg atgagttgtg aaaaacggtg acagtaagag ccctgttggc
      961 ggcgaattcg acgggcgctt acggttctac gtagtaagtt gagttcagca gcgaaatgcc
     1021 cacaggttgc cgcagcctgt gggcattctc aatgaaggct agtttcttca gtgcactctc
     1081 tgaagcaaga cgtccagggc ccagcgcccc atctgagcga cgtgaacgtc gctaaccttc
     1141 tcaaataaat cactctcctt aatgcggctt ttgtaggaac acgtcccagg cccagcgccc
     1201 tacctggccg ccctgcgagt cgttaacctt cgcccgatat ctaaaccagt tcccgttgtc
     1261 gtctttccgg cgcgagtccc tgtacttcaa ctcaattacg cgaacatccg agtccaggag
     1321 gctctgcaac tctcccgatt ctgagatccc gttgtgattg ccatcttgcc agagcctcag
     1381 actcgagaaa acactatcgt taccatcaat ctgaccatcg ccgtttccac cgttatccgg
     1441 atcgtcaaac atcgaaaggg cgcgaaagcc attcttcatt tctccgggga ctaagtatgg
     1501 ctggggggaa gagctgccaa agagctcgcg cccattatca atcacaccgt taccattccg
     1561 gtcgagcacc agccacgcgt catcagagcc ggcggctgtc catgagattt gttcggcaat
     1621 gccggtcgca ttcagatcaa acattacacc gtttgtggcg tttgtgagat caaagccgtt
     1681 acccgcaacg tcgataacga tcggcgaata acacgagcat gtggagtctg accattcgcc
     1741 gccgccatta aaacaagtag actgctcgac cgcatcacaa caataaggat ttatcaggtt
     1801 gggggctggg attcgcatag cggattttgc agtcttgagc ggcgccatat tacaaccgcc
     1861 accaccaccc ccgccgccgc caaatggttt tggaatattg cacgtatgtc cgtgaacgag
     1921 ttggcctcgc cagacgtcgc cgcccgtcat ctgcatactc aggccctcgc cgactaacgt
     1981 ggccgtgaag ttacaattag gatcaataca gttctttgtg gccacaccat acccggaata
     2041 gcagctgatg ggcgttcctc gcggccccga agccgtgcct ttcgattcgt cgtaccggtt
     2101 tctatgacta taaacgaggt tcgttacaga cccgttttca tagacgtaga ttccatagaa
     2161 attaacccgg caagtttgtt gagtagtagt tggtgcttcg caagcaccct gaaacgagat
     2221 cctttctcca tgcgccccgg aagaatgctc tctatagtcc tcagtagtgt tttcaaccac
     2281 ggggccgagt gacggagtat ctgaagaggt ctgtgtggcg gcaagaggag tggttcctcc
     2341 agctttgatt agcaacgtat gtgctaaaac accgctagac gcggaaatag gtgggggcag
     2401 ccgttgcagg ttaaccgatt tatgaggctg cgcttcctca ttcgtttgtc cgggctcacc
     2461 aacagccggt cgttgctcat accaaagact ttcgaaatcc cccgccgaag acacgacggt
     2521 gtcccaaaaa ataggcttaa gactcttgac agaaccaaaa ggattgcgaa acaaagtcac
     2581 tgcactgtag attcgaactt tcccgtggaa tcgaagtgtg acgtcatact tcacatagaa
     2641 tcccacatcg tgatatctcg cagggtcgta actcaacaaa tcattaacag tccacttagg
     2701 attatagagt gctgcgtacc gctcggttgt ccatcgtgcc caataggcga catgtggatc
     2761 cgcatcgttc agcgtttgaa cggatcgacc aagttgaaga atttccgttc ctgccggcag
     2821 tacttcgtcg tgaggcaaat tcaatatctc ctgaatcgcc ccaactctaa agttagacaa
     2881 ttcgaagcgg agataagact cgtcgagcgg tgcgttttcc cggcgcgggt cgtgaacgag
     2941 tagataggct cggttgtaac gagttagttt ggcgtatgcc gctctaacaa gcgtttcagc
     3001 tgcagcctca gacgatacgc cggcttccgt tactgtgtcc ccttgctttg gaccagggtt
     3061 ctgttcctgg gccgaagctg aaatgctcat acctaataac cacaagaaca ataaagataa
     3121 gtttcgggtt ctcatttctg cctcctagca tctggaacaa ccagaccgag ttgatacgga
     3181 tgaggtgcga gcagaagtga gttcctgctc gattgctttc ttaaaaccta tgaaatccaa
     3241 agctccccga a
//
LOCUS       DASUEX010000307         4628 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_63526, whole genome shotgun sequence.
ACCESSION   DASUEX010000307 DASUEX010000000
VERSION     DASUEX010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4628
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63526"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..992
                     /locus_tag="VFV61_05165"
     CDS             <1..992
                     /locus_tag="VFV61_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX4899901.1"
                     /translation="LNQPGDLTFAQLEKVSGTMPPITDLWLSGGEPTLRRDVSEIIDA
                     FVNSNGVRRVIIPTNALIKSRVYEIVDRALARHPKLDLYLNTALDGYGKTHDRIRGVP
                     GNWDKALECIEFLYPLKEQYQARFRLNVNTVVCAENYTEIEKLAEYMWSNFRLDGQYF
                     NIIRGETLVGDEIKQVPPESLPEIYSFVSKLTKRYGDRMFADDEASRRFVKNVAYVGA
                     ITTHYRTQLANFAGPNPWPFPCTAGETTAVIDYNGDVRACELREKFATLADYDYDFEA
                     LWSAQERQAELQGIDGGKACWCTHVCFIHDSLRHSRKGLLYELPKNFVTRGSW"
     gene            1002..1217
                     /locus_tag="VFV61_05170"
     CDS             1002..1217
                     /locus_tag="VFV61_05170"
                     /inference="COORDINATES: protein motif:HMM:NF021073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HEX4899902.1"
                     /translation="MAGREDQEANRKTGLAYAAGINLFASVIALAGLGWLLDRWLATA
                     PWLLVTGVVLGAVVGFYQFIRITSKLK"
     gene            1355..1783
                     /locus_tag="VFV61_05175"
     CDS             1355..1783
                     /locus_tag="VFV61_05175"
                     /inference="COORDINATES: protein motif:HMM:NF015834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HEX4899903.1"
                     /translation="MAEISDKQRPTKSGSTLDTRILRTMALAVGVAVLLSTVVAPWRV
                     TTGLLLGGLLSLLSHHWMRSSIAAAFSGALQAGSKPQLTSVKYILRYFVIAVAVFTAY
                     QFNLVSLPATIAGLCSFVVALFVEALREFYFVIIHREEIS"
     gene            1783..2724
                     /gene="atpB"
                     /locus_tag="VFV61_05180"
     CDS             1783..2724
                     /gene="atpB"
                     /locus_tag="VFV61_05180"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEX4899904.1"
                     /translation="MFWLLETAKAGEHAEQAHETAHHTPIIVELVNHYFGQTAHDLQM
                     KYTYPIWKSVFAKFHTTPEAVFGPYTPETAIPWYTVMFVIACILSVVVIWILKGRLSA
                     EEPGHGQQTLEIAVLAVRSQIADIIGPHGLKYFPIVMTFAVLILVSNLMGLVPLFMSP
                     TAAVSVTFALGITSFVYYNYIGIKENGLFGHLKHWAGPIWWIAPLIFVIEGISNFIRP
                     FSLGIRLFGNMFADEQVAATVAGLYPSVTHWVLPALLMVLGVFVSFIQTFVFILLSQL
                     YLSEVSHAPHDEHDKHGVDEEDAAEEAGALGLAVVPY"
     gene            2984..3304
                     /locus_tag="VFV61_05185"
     CDS             2984..3304
                     /locus_tag="VFV61_05185"
                     /inference="COORDINATES: protein motif:HMM:NF012365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HEX4899905.1"
                     /translation="MRRLNKLFFVIVAVLMAAVPALAQAPAEAAAAGDGGAGLKAIAA
                     GIGFAIAVFGGALGQSRIGAAACEGAARNPGASAKIQTMMILGLALIESLVLFALVIV
                     FVKT"
     gene            3454..>4628
                     /locus_tag="VFV61_05190"
     CDS             3454..>4628
                     /locus_tag="VFV61_05190"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX4899906.1"
                     /translation="MKKCPKCGNEYPDTTTLCPADGVGLETSGDSLLGQTLADKYRID
                     ERLNEGGMGTVYRGTHVLMDKTVAIKVLRPSLAADEKIVARFSREARAASRISHPHAL
                     SVTDFGEAENGVVFLVMEFLNGQTLKNVIRQDGPMPLARVVEIIRQVGAALDAAHEQG
                     VVHRDLKSENIMLLAANGPDYAKVLDFGIAKIKEHDGDYDPGLTAPDLVIGTPQYMSP
                     EQCSQAPDIDARSDIYSLGVIIYEMLVGHVPFTGPSPTAIMMKQLQEPVPSVLVERSD
                     LPAAVGRVISRAMAKQPADRYQTVGELVEDLTIAGGAEAVTTPPVPTTAAANRIVVPT
                     RTSENELIEDEVTVVRRRLETTPGTGAHAVRALPPDAGVNPWKILLPALAGLLVVFAV
                     "
BASE COUNT          960 a         1216 c         1320 g         1132 t
ORIGIN      
        1 agttgaatca gccgggcgat ctcacttttg ctcaactcga aaaagtttcc ggaacgatgc
       61 cgccgatcac cgacctctgg ctctcaggtg gtgaaccgac tctgcgccgc gatgttagtg
      121 agatcatcga cgccttcgtc aacagtaacg gtgtgcggcg cgtcattatt cctaccaatg
      181 cgttgatcaa gtcgcgggtg tacgagattg tcgatcgggc gctcgcccgc cacccgaaac
      241 tcgatctcta tctcaacaca gcgctcgacg gttatggcaa aactcatgat cgcatccgcg
      301 gcgttcccgg aaactgggac aaggccctcg agtgcattga gtttctttat cccttaaagg
      361 agcagtatca agctcggttt cgtcttaatg taaacacggt cgtctgtgcc gaaaactaca
      421 cggagatcga aaaactggcg gagtacatgt ggtcaaactt ccgtctcgac ggccaatact
      481 tcaacatcat tcgcggcgag acattggtgg gcgacgagat caagcaggtg ccgccggaat
      541 ctttaccgga aatctattcc ttcgtttcga aattgaccaa gcgctatggg gatcggatgt
      601 ttgctgacga tgaggcaagt agacgcttcg tcaagaacgt tgcttatgtc ggcgcgatta
      661 ccacgcacta ccgcacacaa cttgctaact ttgcagggcc caatccctgg ccgtttccct
      721 gcacggcggg agagaccacg gcggtgatcg attacaacgg tgacgtgcgt gcctgcgagc
      781 tgcgcgagaa gtttgccacg ctcgctgact acgattacga ctttgaagcc ctctggtcgg
      841 cgcaagagcg gcaagcggaa ctgcagggga tcgacggcgg caaggcttgt tggtgtacgc
      901 acgtttgttt cattcacgat tctttacgtc attctcgtaa aggcttgctc tatgagttgc
      961 cgaaaaattt tgtgacgcgc ggaagctggt agcagaacct tatggctgga cgcgaagatc
     1021 aggaagcaaa tcgcaagact ggcctcgctt acgcagcggg gatcaacctg ttcgcgtcgg
     1081 tgattgcgct tgcggggttg ggctggttgc ttgatcgctg gttagctacc gcgccctggt
     1141 tgttagttac tggcgttgtg ttaggtgcag tggtgggttt ctatcagttc atccgtatca
     1201 cttcaaagct caagtaatcg tcagaagtta ccaccagggc gcagagacac ggagacgcgt
     1261 ctccgcgtca ccgcgtcacc gcgtcaccgc gtccccgtgc tagggctacc tcgacaactc
     1321 cacgtttccc cgtgtaaaat ctcccacttc gcgcatggcc gaaatttccg acaaacagcg
     1381 accgactaag tctgggagca ctctcgacac ccgcatcttg cgcacaatgg cgcttgctgt
     1441 cggcgttgct gtgcttctga gcacggtcgt tgcgccctgg cgtgtgacga caggtttgtt
     1501 gttgggaggc ttgctctccc tgcttagtca ccactggatg cgctcctcaa tagccgcggc
     1561 attctctggt gcgttgcaag caggctccaa acctcagctc acttcggtga aatacattct
     1621 gcgatacttc gtaatcgccg tcgctgtgtt taccgcctac cagttcaacc tggtttcatt
     1681 gccggcgaca attgccggct tgtgttcttt tgtagttgct ttgtttgttg aagccttgag
     1741 agagttttat ttcgtaatca ttcaccgaga ggaaattagt taatgttctg gttgttggaa
     1801 actgccaaag ctggcgagca tgcggaacag gctcacgaaa ctgctcatca cacgccaatt
     1861 atcgttgagc tcgtcaacca ttacttcggc cagacggccc acgacctgca gatgaaatac
     1921 acgtatccga tctggaagtc ggtcttcgca aaatttcata cgacgcccga agcggtattt
     1981 ggtccttaca cacctgagac ggccattccc tggtacaccg tgatgtttgt gatcgcctgc
     2041 atcctgagcg tcgtcgtgat ctggattctt aaaggcagac tttctgcgga ggagccgggg
     2101 cacgggcaac agacgttaga gattgcggtt ctcgcggttc gctcccagat tgccgacatc
     2161 atcgggccgc acggattgaa gtactttccg attgtaatga catttgccgt gctgattctg
     2221 gtttcgaatc tgatgggatt ggttcccctg ttcatgtcgc ccacggcggc cgtcagcgtc
     2281 accttcgcgc tggggatcac gtcgtttgtt tattacaact acatcggcat taaagagaat
     2341 gggctttttg ggcacctgaa acactgggct gggccgatct ggtggattgc gccactgatc
     2401 ttcgtcattg aaggcatcag caacttcatt cgcccgttct cgctcggtat tcgtttattt
     2461 ggaaacatgt ttgcggatga gcaggttgct gccaccgttg ccggcctcta tccctcagtg
     2521 acgcactggg tgctgcctgc cctcctaatg gtgctgggcg ttttcgtctc gttcattcaa
     2581 acgtttgttt tcattctgct ttcgcagctc tatctcagcg aggtttcgca tgcaccgcac
     2641 gacgaacatg acaaacatgg cgttgacgaa gaggatgctg ctgaggaggc cggggcgtta
     2701 ggattggcgg tagttcccta ttgattcgga gttataaaaa acatgcgtcg agcggtaccg
     2761 taatgcacgg gcgacacgtt gacgcataag gattgacttg gtgaacgaaa agaaaatggc
     2821 tgtggatcat cgcttcgtta tttgaagcaa tagtgtctgt ctcgctggag cgccgttgta
     2881 agaggggcgg tggataccgg acgcaggcga aataatacaa gctggccaca tttctttccc
     2941 gtgcaccatt ttgattctga ttaaattcag aaggagattg aagatgagga gacttaacaa
     3001 gcttttcttt gtcatcgtag ctgtcctgat ggctgcggtg cccgctttgg ctcaagcgcc
     3061 ggcagaagca gcggcggcgg gtgacggcgg cgccggcttg aaggcgatcg ccgccggcat
     3121 cggtttcgcg attgcagttt tcggcggcgc gctgggccaa tcgcgcattg gcgctgcggc
     3181 ctgcgaaggt gctgcgcgca accccggagc ttcggcaaag attcagacga tgatgattct
     3241 gggtctggct ctaatcgaat cgctggtgct tttcgcgctc gtgatcgtgt tcgttaagac
     3301 ttaacaacca acgcagaggt aaaccaacgt gagcggagct tactgcttcg ctcgcgtttt
     3361 tgttttgagt ggccatgctt ttggtagtct tgaagcggtg tggtaggctg acagctctcc
     3421 caaacaagac agtgacgagg cacaaatcgc ccgatgaaga aatgtccgaa gtgtggaaat
     3481 gaatatccgg acacaaccac cttgtgtccc gccgacggcg ttggcttgga aacgtcgggc
     3541 gattccttgc tcggacaaac tctcgctgac aaatatcgta tcgatgagcg cctgaacgaa
     3601 ggaggcatgg gcacagtcta ccgtggcact catgtgctga tggataagac ggtggccatc
     3661 aaggtgctgc gtccttccct cgctgccgac gaaaagatcg tcgcgcgttt ctctcgcgaa
     3721 gcccgcgccg cttctcgcat ttcccatcct cacgcactga gcgtcacaga ctttggcgaa
     3781 gctgaaaacg gtgtcgtgtt tctggtaatg gagtttctga atgggcaaac gttgaagaac
     3841 gtgattcgtc aggatggacc gatgccattg gcgcgagttg tggagatcat ccggcaggtt
     3901 ggggccgctc tcgacgcggc acatgagcag ggagtggtgc atcgtgatct aaaaagcgag
     3961 aacattatgt tgctcgccgc taatggaccc gattacgcga aagtcctcga ctttggcatc
     4021 gctaagatta aggaacacga cggcgactac gatcccggac taactgcgcc ggatctggtt
     4081 atcggcacac cgcaatacat gtcgccggag cagtgctcgc aggccccgga cattgacgcg
     4141 cgttcggaca tctattcgct cggcgtgatc atttacgaga tgctcgtggg acatgtgcct
     4201 tttaccgggc cctcaccgac ggcgatcatg atgaagcagc ttcaggagcc cgtgccttcg
     4261 gttctggttg agagaagtga tctgccggcc gcggtgggcc gtgtgatttc gcgagcgatg
     4321 gccaagcagc ccgctgatcg ttatcaaacc gtcggtgaac tcgttgagga tcttacgata
     4381 gccggcggcg ctgaggcggt aacgactccg cccgttccca cgacggcggc cgctaatcgc
     4441 atcgtggtgc ccaccagaac ttctgaaaat gaattaatcg aagacgaagt caccgtggtc
     4501 cgtcgtcggc tggaaactac gccgggaaca ggagcgcatg cggtgcgcgc gttgccgccg
     4561 gatgctggtg ttaatccctg gaagattttg cttccggcgc tagcaggatt gttggtggtc
     4621 tttgctgt
//
LOCUS       DASUEX010000308         5336 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1553444, whole genome shotgun sequence.
ACCESSION   DASUEX010000308 DASUEX010000000
VERSION     DASUEX010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5336
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1553444"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(361..1104)
                     /gene="rnc"
                     /locus_tag="VFV61_05195"
     CDS             complement(361..1104)
                     /gene="rnc"
                     /locus_tag="VFV61_05195"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEX4899907.1"
                     /translation="MKTSANNSSDQDLLESALAYNFRNRALLERAVTHRSWAHEQVAP
                     GNEELARQLHNEALEFLGDSVLGLIVADYLCRAHPEVTEGELSRMKHRLVSAPTLAQA
                     SLRLNLGDFLRFGRGEERSGGRNKDALLADVLEAITGAIFLDGGLAAATDFVQRAIGD
                     ELKRATPAAAAEADYKTMLQERLQAERRPAPRYKVVETLGPPHRRTFRVEVSWDEGSV
                     SGEGRSIKSAEVAAAKLALEAMASGETGE"
     gene            complement(1194..2393)
                     /locus_tag="VFV61_05200"
     CDS             complement(1194..2393)
                     /locus_tag="VFV61_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899908.1"
                     /translation="MKKIIELIAVVAVLAVPVFAGGSSQPVNTAQDQCTAENKLAWYN
                     EFRQHFKGDTAKANELAKKWLACPEAAGEEQQAAYLKNFVTLFEKANRKGQINDLVYG
                     AKKDYVKAFELGRQVLAEEPENLKVLIDLAYAGYASKNAALAADSLSYAQKAIRMIES
                     GKTLEAWTPYMSREDTLGYLYNATGSLNLQKDPGVALKDFIKAAQFNGKIGKLPATYA
                     TIAQTYEASAYAKLASDYEEKYKGKDETPESKLAVENINQVVDRMIDAYARAVALAGN
                     EPAEQANKKLWMEQLTIWYKYRNKSEAGLPELIAGILAKPMPGEPTPLTVLPVSASGP
                     ATTPTADTASASGVPASGSATKPATANGAAVKPAATTTSATSSSTTGTTKPAAKPKPK
                     RNHTRRG"
     gene            complement(2751..4349)
                     /locus_tag="VFV61_05205"
     CDS             complement(2751..4349)
                     /locus_tag="VFV61_05205"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEX4899909.1"
                     /translation="MTARERRSSRRERYSARNRRGGSRVTARLSRRELRQRRQQIARE
                     QASSLKALERRLRRPLTRRERTAELRRIEARHRRDTLAARRRAEAARRAAIARQRAIE
                     QSMRDEVQASISRDDTTGEDMEVRRAAVNALGNHIGTVVVMDPQSGRVYSIVNQEWAL
                     RRGFKPCSTIKLVTGVAGLGEKVIDPANTAEVSERYNIDLTDALAYSNNTYFQQVGGR
                     VGFNKMMTYAREMGLGEKTGINAPNEFSGRLPFEKSGFALNRMSSHGDDFEVTAVQLA
                     TLVSAMGNGGHLLMPSIPRTPQDAAKFQGKVRRQIEVEPETWRRMVPGMVGAVNYGSG
                     KKAYDPMQTVAGKTGTCIGQGGWVGLFTSYAPLANPRLAVVVIGRGPDARGHFPAAVA
                     GRIYRELNHRFGTPVNLQFAQAPAQEDPKAADLNEEDKDAAEAEAAEQEAIEVQANAE
                     AEASVKAEQQTAAGSTAEPAKPHRTDNTVIRNPVKRVLMPINRPVEAPKTATKPVSTP
                     NTTTPAVKSTAPSSETRPRRAQTN"
     gene            4997..>5336
                     /locus_tag="VFV61_05210"
     CDS             4997..>5336
                     /locus_tag="VFV61_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899910.1"
                     /translation="MKISGLLNSFRARLLLLLAVMLGLTLGVQYYVNLRAIQTNAQVI
                     VEQEQAIMAGVALGVNSIFSTKYLDELRGNLRVPLLDEREGRVKNVMVVDSDGLVQDS
                     LVSDYAPTENE"
BASE COUNT         1071 a         1480 c         1487 g         1298 t
ORIGIN      
        1 aagaaaacgg acgagcctgg ggcttcgtct cggtgtcgat gttagaagtt ttcgtctccg
       61 gaaggttgct actcatctta aatcatccta gatacgttga agggcacggt caaggttgca
      121 ctcgtggtat tggcggttgc cgattgccaa ttggcgattg ccaatttcgc tttagcctcg
      181 ctccgctaca gaattggggg gtgcttcgag gcggtcgcta tgctcttgga ctgtagtggg
      241 tggtcacgcg ctggaatgca aggcattaga gcgatccaat ccgttcccct gttccccctt
      301 ccctgtttcc ccgtttcccc tctgttaccc agtttcccgt tcccctgttt ccctgttccc
      361 ctattccccc gtttccccgc tggccatggc ctccaacgcc agcttcgccg ctgctacttc
      421 tgcactcttg attgaacgtc cttccccgct gacgctgcct tcatcccaac tcacttccac
      481 tcgaaaggtc cggcgatggg gcggacccaa tgtttccacc actttgtacc ggggcgctgg
      541 ccggcgctct gcttgcagcc tttcctggag cattgttttg taatctgcct cggccgccgc
      601 cgcgggagta gctcgcttca attcgtcgcc aattgcccgt tgcacaaaat cagtcgccgc
      661 cgcgagaccg ccatcaagaa aaatcgctcc tgttatcgct tccagcacat cagccagcaa
      721 agcatcctta ttgcggccgc cgctcctttc ttcaccgcga ccaaagcgga ggaagtcgcc
      781 gaggttaagt cggagcgagg cctgcgcgag tgtgggagca cttacgaggc gatgtttcat
      841 gcgtgagagc tcgccttctg taacctcagg gtgagcgcgg cagagatagt ccgcgacaat
      901 caatcccagt actgagtcgc cgagaaactc aagagcctca ttgtgcagtt gccgggccag
      961 ttcttcatta ccgggagcga cttgttcgtg cgcccaggaa cgatgagtca cggcgcgttc
     1021 aagcaaagcc cgattgcgaa agttataagc cagcgctgat tccagaaggt cttgatctga
     1081 agagttgttg gcggaagtct tcaaggtggg ttcgattcta acaaaacgcc ggaagcatgg
     1141 tcgaagttac gactaagctt ccggcgccga tctggaggcg gaaagaataa cacttaccca
     1201 cgtcgcgtgt gattacgctt cggtttaggt ttagctgccg gcttggtggt tccggttgtg
     1261 ctgctgctcg tcgccgaagt ggtggtagcc gcaggtttaa ccgcagcgcc gttcgctgta
     1321 gctggtttgg ttgcagaccc cgaagctgga actccagaag ctgaggctgt atcagccgtc
     1381 ggagttgtcg ctggaccact cgccgaaacc ggcagcacgg ttagcggggt gggttcgccg
     1441 ggcatcggtt tcgccaaaat tccagcaatc aactcaggca gtccagcttc cgatttattg
     1501 cggtacttgt accaaatggt gagctgctcc atccacagtt tcttgtttgc ctgttcggct
     1561 ggttcattcc ccgcgagcgc gacggcgcgc gcataggcgt cgatcatccg gtcgacgacc
     1621 tgattgatat tctcaacggc caatttactc tcaggcgttt catccttgcc cttgtatttc
     1681 tcttcgtaat ccgacgccaa cttcgcgtag gcgcttgcct cgtaagtctg agcgatcgtc
     1741 gcataggtcg ccggcagttt gccgatcttg ccgttgaact gagccgcttt aataaagtcc
     1801 ttcagcgcga cgcccgggtc cttttgcaag ttaagcgaac cagtggcgtt gtagaggtag
     1861 ccgagagtgt cttcgcggct catgtaggga gtccaagcct cgagcgtctt tcctgattcg
     1921 atcatgcgaa tggctttttg cgcataactc agactatcag cggcgagcgc agcattcttc
     1981 gacgcataac cggcatatgc cagatcaatc aaaaccttga gattctctgg ttcctctgcc
     2041 agaacctgcc ttcccaactc aaaagctttg acgtaatcct tcttggcacc gtaaaccaga
     2101 tcgttgattt ggcctttgcg attggccttc tcgaaaagcg taacaaagtt tttcagataa
     2161 gccgcttgct gctcttcgcc ggcggcttcc ggacacgcca gccacttctt ggccagctcg
     2221 tttgccttag cggtatcacc tttgaagtgt tgtctgaatt cgttgtacca ggcgagcttg
     2281 ttctcggccg tgcactggtc ctgggctgtg ttgactggct gactgctgcc accggcgaag
     2341 accggtacag caagcactgc aaccaccgca atcaactcaa taatcttttt cattcgtttg
     2401 ttcccaacct ccaagatcat ttctgcattc taaactctgg gcctcgagcg acttttccgt
     2461 agtgtcgcac gcgcgacaat tgagatgcgt tcgtagaaag accggttgcc gatcttcaca
     2521 tctcgaagga ggaacctcgc aggggtgtgg agaaatgtcg ggccttaaga accgtattgc
     2581 taagaacgcc cgcaatattc ctgcaagtct cgctaacgtg tcaagggcgt taggagcgtt
     2641 tactttggcc gaggacgtgt cagccaaaaa ccttgagcgc cggttccatt catggggttg
     2701 ccggcgctcg tggtatttcg aagaattgca atcaagttgc agctaaacag ctaatttgtt
     2761 tgtgcccgac gcggcctcgt ttctgacgac ggcgcggtac tttttacggc cggggtagtc
     2821 gtgttcggcg tgctcaccgg tttcgtggcg gtcttcggcg cttcgaccgg gcgattaatt
     2881 ggcatcaata cacgcttcac tggattgcgt atgaccgtgt tatccgtgcg atgtggctta
     2941 gctggctcag cggtgctacc ggctgcggtc tgttgttctg ccttgaccga tgcttcggcc
     3001 tcggcattag cctgaacttc gatggcctct tgctcagccg cttccgcttc cgctgcgtct
     3061 ttatcttcct cgttcaaatc cgctgctttg ggatcttcct gagccggtgc ctgggcgaac
     3121 tgcagattaa ccggcgtgcc gaagcgatgg ttcagttcac gatagattcg gccggcgacg
     3181 gcggctggga aatgtccacg agcgtcgggg ccgcggccaa tcacaactac tgctaggcgg
     3241 ggattcgcaa gcggggcgta cgaggtaaag agtcctaccc aaccaccttg accgatgcag
     3301 gtaccggtct tgccagccac ggtttgcatc ggatcataag ccttcttacc tgagccgtaa
     3361 ttgacggcgc caaccattcc cggaaccatg cgacgccagg tttcaggttc gacttcaatt
     3421 tgccggcgta ctttcccttg gaactttgct gcatcttgcg gtgtgcgcgg gatgctcggc
     3481 atcaggaggt gaccgccgtt gcccatagca gaaacgagcg tggccagttg caccgcggta
     3541 acttcaaagt catctccgtg cgacgacatg cggttgagcg caaagccact cttttcgaag
     3601 ggcaagcggc ctgagaattc attaggagcg tttattccag tcttctcccc taaacccatt
     3661 tcgcgcgcgt aggtcatcat cttgttgaaa cccacccggc caccaacctg ctggaagtaa
     3721 gtattgtttg aatatgcgag ggcatccgta aggtcgatgt tgtatctctc ggagacttcg
     3781 gcagtgttgg caggatcgat gaccttctct cccaagccgg cgacgccggt gacgagctta
     3841 atcgttgagc atggcttaaa gccgcgacgt agagcccact cctgattaac tatggaatag
     3901 acgcggccgc tctgcggatc cataacaacg actgtgccga tgtgattgcc gagggcatta
     3961 actgccgcgc gtcggacttc catatcttca ccggtggtgt cgtcacggct aatgcttgcc
     4021 tgcacttcat cgcgcatgga ttgctcaatg gcgcgctggc gggcgatggc cgcgcggcgc
     4081 gcagcttcag cacggcgacg ggccgcaagc gtatcgcgac gatgacgtgc ttcgattctg
     4141 cggagttcag ccgtgcgttc acgccgggtc aacgggcggc gcagtctgcg ctcgagggct
     4201 ttaagcgatg aggcttgctc gcgagcgatt tgctgacggc gttggcggag ttcacgcctc
     4261 gagagacgag ccgttactcg ggacccaccg cggcgatttc gtgctgaata tctctcgcgc
     4321 cgtgaggagc gacgctcgcg ggcagtcatt tttcggctgc gatcagcacg ggacgatctg
     4381 tgacgggccg acttgtcgcg agtggatttg gattttgctg ctttgttgga cgaggctgct
     4441 cgtcgcttgc cgaatacttc ggtggataaa gacagggata agagaacaag taggattaag
     4501 acgaaacgcg ggcttacaaa cgaacgggta aatcgggaag acggggaatc aaagggatgg
     4561 gtcggttggt tcatgcggcc tgttaccttt cctaggaaat tacgctcatc aggaatgagc
     4621 ttcgccaaaa ctaataagga acctcgtcca acctaattca aactggacga tgcaccctct
     4681 cctcggtaag aactcctgca agttttgaga gcactgcatt gctctctcaa aaatatcaat
     4741 tcccggcgcc ggacaaccct gtttttagcg gaccaatcaa tctgcaactt gaggtgggag
     4801 aattccctta aggcggcccc tggggacagt tcaacgaggc caagggcaat ataacaccag
     4861 ccaatccctc cagcaacata aaagtaacct tttgtacata aaccgttaag agggtttgga
     4921 atgcagtttt ttgtgagcta agctggccgc aaatggtgta gggaatcccc tctattctcc
     4981 acgtcgcagg cgcaacatga agatttccgg acttttgaac agctttagag ccagattgct
     5041 gctgctactc gcagtgatgc tggggctgac tttgggggtg cagtactacg tcaatttgcg
     5101 tgcgattcag accaacgccc aggtaatcgt ggaacaggaa caggccatca tggccggtgt
     5161 ggccctcggc gtaaacagca tcttcagcac aaagtacctg gatgagctgc ggggcaacct
     5221 gcgtgtcccc ctgcttgatg agcgggaagg tcgcgtcaag aacgtaatgg tggtcgatag
     5281 cgacggcctc gttcaggaca gcctggtgag cgactatgcg cccacagaaa acgagg
//
LOCUS       DASUEX010000309         2368 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1301612, whole genome shotgun sequence.
ACCESSION   DASUEX010000309 DASUEX010000000
VERSION     DASUEX010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1301612"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1254
                     /locus_tag="VFV61_05215"
     CDS             <1..1254
                     /locus_tag="VFV61_05215"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HEX4899911.1"
                     /translation="HRLSLASIVLNIGVSVLMASESLIAICAMIVGTLSVVLATPLVA
                     IANGANWMMLHSVDPFAYLGLASIRLPEYSGWAAGIYFLYYLPLTLLTLQLMRWQPLK
                     PPKRAFRTRCWSGLPLMGYGVLSTTILLHPFSTGSTGGRLRIDFLDVGQGDSALLTMP
                     DGTTLLIDGGGRPDFTRTEQRDLFERDTRSIGEAVVSEYLWWRGIDSVDYVLATHADA
                     DHIDGLNDVVRNFIVRAALVGRTPSDDPEYAKFSVTTEMQSVPVVVTSSSDTLLFGDV
                     SARVLWPPPMTKQNAPSQNNDSVVLQVRYGNRQMLLTGDIEKGAESSLVRRGADLRSD
                     VVKVAHHGSKTSSIDGFVQATSPKLAIISVGERSIFGHPNREVVTRWQASGAEVLITG
                     KCGTITVSTDGTKLLVETFLNRSGD"
     gene            complement(1274..2134)
                     /gene="modA"
                     /locus_tag="VFV61_05220"
     CDS             complement(1274..2134)
                     /gene="modA"
                     /locus_tag="VFV61_05220"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX4899912.1"
                     /translation="MSSVERQCPMSAEAKDFGIWTLVAGLVFALALISLTACKPNLIQ
                     TTKQITVAAAADLGPAFDEIGRAFEAESRTKVIFSFGSTGMLTQQIENGAPMDVFAAA
                     DVSYIDQLERKGFIVSGTRAIYALGRITIWTRKTAALKPQQLAELTRPEVKRIAIANP
                     GHAPYGLAARQALEAAGVWDEVKPKLIYGDNIRQALQFAQTGNVDVAIVALSLSLPSD
                     GYWVLIPAELHKPLNQGLGVVKGTAHESDARNFASFVNSAAGRKIMQKYGFTLPGDEP
                     VPQASAQASP"
     gene            complement(2141..>2368)
                     /locus_tag="VFV61_05225"
     CDS             complement(2141..>2368)
                     /locus_tag="VFV61_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899913.1"
                     /translation="FDEEKGLCYKSARERFQGEVTGADVPDIHQLGNPDVSEQNLITI
                     HIYAPPLGVLKTYKVGSAEIDTYTPNDADVQ"
BASE COUNT          512 a          633 c          660 g          563 t
ORIGIN      
        1 caccggctct cattggcttc aatcgttttg aatataggtg tcagtgtcct gatggcttca
       61 gaatcgctta tcgccatatg cgccatgatt gtcgggacac ttagtgtggt gctggcgaca
      121 ccattggttg caatcgcaaa tggtgcaaac tggatgatgc ttcacagtgt ggatccattt
      181 gcttatttgg gacttgcatc cattcggctg cctgagtatt ccgggtgggc agcaggaata
      241 tactttctct actacctccc acttacgctg ctgacgctgc aactgatgcg ttggcaacca
      301 ttgaagccgc ccaagagagc attccgaact cgatgctggt caggattgcc gttgatgggc
      361 tatggcgtgc tctccacaac tattctgctc cacccgttca gtactggtag cacaggtggg
      421 cggctccgga tcgattttct cgacgtgggg cagggtgata gtgcgcttct cacgatgcct
      481 gacggtacta cgttactgat cgatgggggc ggccggcctg actttacaag aactgaacag
      541 cgagacctct tcgagcgcga cacacgctcc atcggtgaag ccgtcgtatc agaatacctt
      601 tggtggcgcg ggatagattc ggttgactat gttctggcta cccatgcgga tgcagatcac
      661 atcgacggac tgaatgacgt ggtccgaaac tttatcgtgc gcgcggcgtt ggtgggtcga
      721 acgccatcgg acgatcccga atacgcgaaa ttcagcgtta ccacggaaat gcagtccgtt
      781 ccagttgtcg tgaccagtag cagtgatacg ttgcttttcg gggatgtcag tgcgagggtc
      841 ctttggccac cgccgatgac gaaacagaat gccccttcgc aaaacaacga ttcagtagtg
      901 ctgcaagtcc gttatggcaa ccgccaaatg ctactaaccg gagatatcga aaaaggcgcc
      961 gaatcatctc tcgtccggcg gggagcagac ttgcgctcgg acgtagtgaa ggttgctcac
     1021 cacggcagca agacgtcctc catcgacggg tttgtgcaag cgacatcgcc caaactggcg
     1081 atcatctcag tcggagagag gtcgatcttt ggtcatccaa accgagaggt ggtcacacgc
     1141 tggcaggctt ctggagcgga agtgttgata accggaaagt gcggcacgat tacagtcagc
     1201 actgacggca cgaaactcct ggtcgagact tttctaaaca ggtcagggga ttaagaggga
     1261 accaaggccg atcctaaggc gatgcttgcg ccgatgcttg cggaaccggc tcatcacccg
     1321 gcagcgtgaa tccgtacttc tgcatgattt tccgtcccgc ggcgctattt acaaatgatg
     1381 caaaattcct ggcatccgat tcatgcgctg tgccctttac cacgccgagt ccctgattca
     1441 acggcttgtg tagttccgcg gggatcaaaa cccagtatcc atcgctcggc aggctgagtg
     1501 acaaggcaac aatcgccaca tccacatttc ccgtttgcgc aaattgcagg gcctgacgga
     1561 tgttgtcgcc ataaatcagc tttggtttca cttcgtccca gacaccggca gcttcgagcg
     1621 cctgccgagc ggccagcccg tatggcgcat gacccggatt ggcaattgca atgcgtttta
     1681 cctcgggccg tgtcagctcg gcgagctgtt gaggtttgag agccgcggtt tttctggtcc
     1741 aaatggtgat gcgtcccagc gcatagattg cgcgcgtacc ggaaacaatg aaccccttgc
     1801 gttcaagctg atcgatgtat gaaacgtctg cggctgcgaa aacgtccatc ggagcaccgt
     1861 tttcaatctg ctgcgtcaac atgcctgtgg aaccaaagct gaatatgact ttggttctgg
     1921 actcagcttc gaacgcacgg ccgatctcat cgaacgccgg tccgagatcc gctgcggcgg
     1981 ccacggttat ctgcttggtt gtctggatta ggttgggctt gcaagccgtg aggctgatca
     2041 aagcgagcgc aaaaacaagt ccagcgacca acgtccagat tccaaagtcc tttgcctcag
     2101 cggacattgg acattgacgt tcgacgctgg acattgaacc ttactggacg tcggcgtcgt
     2161 tgggggtgta ggtatcaatc tcggcgcttc cgactttgta ggtcttgagc acgcccagcg
     2221 gtggcgcata aatatgaatg gtaatcagat tttgttcgct cacgtccggg tttcccaact
     2281 ggtgaatgtc tggcacatcg gcaccggtaa cttcgccctg aaagcgttcg cgagctgact
     2341 tgtagcaaag acccttttct tcgtcgaa
//
LOCUS       DASUEX010000310         2700 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_981215, whole genome shotgun sequence.
ACCESSION   DASUEX010000310 DASUEX010000000
VERSION     DASUEX010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2700
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_981215"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="VFV61_05230"
     CDS             <1..543
                     /locus_tag="VFV61_05230"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX4899914.1"
                     /translation="LEFTRMTCELIKNIRTLPQPVIAKLNGTTAGAGACIALAADIRI
                     ASETAKIAFLFVKVGLSGADMGAAYLLPRVIGLAKATELLYTGDFISAQEAERMGLYN
                     QVVAAEQLDEACNEFAQRLAAGPAFALAKTKEMLNRELDMNLEQALECEAQAQAICMQ
                     HPDYREAYEAFVAKRSPKFE"
     gene            695..1852
                     /locus_tag="VFV61_05235"
     CDS             695..1852
                     /locus_tag="VFV61_05235"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX4899915.1"
                     /translation="MDSFINLPFFNNDHRELAARVSTFVAQEIEVPARAGRDLDNRAR
                     DYVAALARAGLLRYAVPAHDAPLDVRSLCLIRETLSYSSSLADLAFVMQGLGTHAISQ
                     AAPEHVRDFWLTRAAEGRAIAAFALTEPEAGSDVAAVKTTATRDGDSWVINGRKRFIS
                     NAGIADFYTVFARTGTQTGGRADLSAFVVGARMPGFRLVERTALISPHPIGEIEFRDC
                     RVPAEDMVGAEGDGFLLAMRTLDTFRASVGAAACGMARRALVESVRYASSRKQFGRLL
                     AEHQLIQEKLADMVTELDAARLLVYRSAYLKDSGGGRVSREASEAKLFATEAAGRIID
                     QAVQIHGGSGLVSGAVVERLYRDVRALRIYEGTSEIQKLIIASQLLKDSTN"
     gene            1996..>2700
                     /locus_tag="VFV61_05240"
     CDS             1996..>2700
                     /locus_tag="VFV61_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899916.1"
                     /translation="MKINIIGGGPAGLYFAILMKLADPAHEIAVWERNGPDDTFGWGV
                     VFSGTTLSNLRAADVVSHAEITRDFAAWDNVDVLHGGDKISIHGNSFAGISRLHLLKI
                     LQRRCEHLNVELNFRSEVINIAALAEDCDLLVGADGVNSTVRENYKLQFQPDLSARPN
                     KYIWYGTHQLFHGLTLTFRENDAGVFAAHSYKFNATTSTFIVECDHETWSKAAFATKD
                     DVTTKTYLEAVFAQDLA"
BASE COUNT          591 a          730 c          761 g          618 t
ORIGIN      
        1 ctggagttta cgcggatgac atgcgaatta attaagaata ttcgcactct gccgcagccg
       61 gtaatcgcga agttgaatgg caccactgcc ggcgccggcg catgtatcgc cctcgccgct
      121 gacattcgca ttgcgagtga aacagccaag atcgcattcc tgtttgtaaa ggtgggtctc
      181 tcaggagcag acatgggcgc ggcttatctg ctgccgcgag ttattggcct ggcgaaggcg
      241 accgagttgc tgtacaccgg cgacttcatt tcagctcaag aggcggaacg aatgggcttg
      301 tacaaccagg tggtagcagc agagcaactc gatgaagctt gcaacgagtt tgcgcaacgg
      361 ctagctgcgg ggcccgcttt cgctttggcg aaaaccaagg agatgctgaa tcgggaattg
      421 gacatgaatc tcgagcaggc gcttgaatgc gaagcccagg cacaggcgat ttgcatgcag
      481 caccctgatt accgcgaagc gtatgaggcg tttgtggcga aacgaagtcc gaagtttgaa
      541 taaagacgta ctttgtactt ggtgctttgt actttgttca cctgctgccg cttgcaccct
      601 tcagggaaac gcaccaagtt tgggaagcga acaaagtaca aagcactaag tacaaagatc
      661 aaagaaagaa cttctaaagt aatagagtga ttcgatggat tctttcatta accttccttt
      721 cttcaacaac gaccatcgcg aactcgctgc gcgggtctcg acttttgttg cacaggaaat
      781 tgaagtccct gcacgcgcgg gacgcgacct tgataatcgc gcccgtgatt acgtagctgc
      841 gctcgcgcgt gctggcttgc tgcgttatgc agttccagca cacgacgctc cgctcgatgt
      901 acgctcgttg tgcctgatcc gggaaacgct ttcctactcg tcctcgcttg cggatcttgc
      961 gtttgtgatg caagggctcg gcactcacgc catcagtcag gcagcgccgg aacacgtacg
     1021 ggatttttgg ttgactcgtg ctgcggaagg acgagcaatt gccgcgtttg ccttgaccga
     1081 gcccgaagcc ggatcggatg tcgcggcggt gaagacgacc gcaacacgcg acggcgattc
     1141 ctgggtaatc aacggcagga agcgattcat ttccaacgcc ggcatcgctg atttctacac
     1201 tgtgttcgca cgcacgggga cgcagaccgg tggacgcgcg gatctttcag cctttgtcgt
     1261 tggcgcgagg atgcctggat ttcgcctggt ggagcgcacc gcgctgattt cgccgcatcc
     1321 catcggcgag attgaatttc gcgactgccg cgtgccggcg gaagacatgg tcggcgccga
     1381 aggcgatggc tttctgctcg cgatgcggac gctcgatact tttcgcgcga gcgtcggcgc
     1441 ggctgcctgt ggcatggcac gacgcgcgct tgttgaatcg gtccgttatg caagctcgcg
     1501 caaacagttt ggccgtttgc tcgccgaaca tcagttgatt caagagaagc tcgcagacat
     1561 ggtgaccgag cttgatgccg cgcgcttact cgtctatcgc tccgcttacc tgaaggattc
     1621 cgggggtggg cgcgttagtc gtgaagctag tgaagcaaaa ttgtttgcga ccgaagctgc
     1681 cggccgcatc attgaccagg cagttcagat tcacggcggc tcgggtttag tcagcggcgc
     1741 cgtcgttgag cgtctctatc gcgacgttcg cgcgctgcgc atctatgaag gcacttctga
     1801 aattcaaaaa ctaattattg ccagtcagct attgaaagac tcgacaaact aaacgcaccg
     1861 tttaaccgct aaggcgcaaa gacgcgaagg cgccgagttt tcttgagcag tagagcggtc
     1921 cattaacttt ttgcgatctc tttgcgtctt gcgccttcgc gtctcggcgc ttagttctca
     1981 gcctgcgaac aaacgatgaa aatcaacatc attggcggcg gtcctgcggg attgtatttc
     2041 gcaatcctga tgaagctggc agatccggca catgagatcg cggtctggga acggaatgga
     2101 cccgacgaca catttggttg gggcgtggtc ttttccggta caacactatc caacctccgc
     2161 gccgcggatg tcgtttccca tgctgagatt acgcgcgact tcgcggcctg ggacaacgtg
     2221 gacgtattgc atggcggcga caagatttcg attcatggca atagctttgc cgggatctca
     2281 cgtctgcacc tgctcaagat cctacagcgc cgctgcgagc accttaacgt cgaactaaat
     2341 tttcgcagtg aagtcattaa cattgccgcc ctcgccgagg attgcgacct gctcgtggga
     2401 gccgacggtg tcaacagcac ggttcgagaa aattacaagc tccagtttca acccgacctt
     2461 agcgcgcggc ccaacaagta catttggtat ggcactcacc agctgttcca cggtttgaca
     2521 ttaacgttcc gggaaaatga cgcgggcgtc ttcgctgcgc attcttataa gttcaatgcc
     2581 actaccagca ctttcatcgt cgagtgtgat cacgaaacgt ggagcaaagc cgcatttgca
     2641 acaaaggacg atgtcacaac aaaaacctat ctcgaagccg tctttgcgca ggatcttgcc
//
LOCUS       DASUEX010000311         2597 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1642954, whole genome shotgun sequence.
ACCESSION   DASUEX010000311 DASUEX010000000
VERSION     DASUEX010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1642954"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            152..853
                     /locus_tag="VFV61_05245"
     CDS             152..853
                     /locus_tag="VFV61_05245"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX4899917.1"
                     /translation="MNLDAAAAELSDLLQQTQEQLLYLQELGISGVEPATSPPELPTQ
                     ANETLEQIWAELKGCTRCPLHEGRTHVVNTEGNPRARLMFVGEAPGADEDSQARPFVG
                     RAGQLLTKIIEAIGFKRGDVLIGNVNRCRPPANRAPLPDEAATCKPFLLREIASVKPD
                     VIVVLGNTAMKNLLDTKIGITRLRGEFQDFNGIKVMPTFHPAYLLRDPSKKRETWEDL
                     KKVRDYLERTAKRAT"
     gene            919..>2597
                     /gene="priA"
                     /locus_tag="VFV61_05250"
     CDS             919..>2597
                     /gene="priA"
                     /locus_tag="VFV61_05250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEX4899918.1"
                     /translation="MPQKFGSSPQATAISPASSEHELKAPLYVEVAVPLHVSQTFTYR
                     LPETAGELAQVGSRVLVPLGRSAVTGFIVELLPDLSSHRTLTDSEIKPVERVLDATPL
                     VTPEVLEITRWVSQYYGSPWGEVLKGALPPGINPNSEQLFTLTANGSARLADLESDEA
                     SSEATVLRAIADAGELNVEAIATLIGKREAIRTTKELQNRNLIVIQLKSGQASARPRT
                     RRVAKLRQPLPTEALSPRKQTAAQQRILDALSNQETMPLAELLRITGVSLSAVNTLHK
                     QGQLEILEESVRRDPLGKVPLPRADEYQLTGAQRQVLSEIEPQLRSGVYAAFLLHGVT
                     GSGKTEVYMRAMRICLELGRAAMMLVPEIALTPVFSRRLRSNFGDQVAIFHSSLSKGE
                     RFDEWMRVKTGRARIVIGTRSGVFAPIANLGLVIVDEEHESTYRQQDSPRYNGRDTAI
                     VRAQKENAVVILGSATPSLESFHNAQTGKYHYLQLPSRIGDRPMAAARIIDMREAFAR
                     HNKPQVFSDELLEAIETTHQKGEQSIILLNRRGYSSFVLCRSCGETIQCPN"
BASE COUNT          618 a          736 c          698 g          545 t
ORIGIN      
        1 gtcggaatga tatcggcttc gacaacgaaa tgaatgcggt cacgattctt acgcaagacg
       61 caaaacccag agaactcccg gcgatgacca aacttgagac ggcccacttt atcctcgatg
      121 aaatagttcg tttgcgaaaa tccaagtcac gatgaatctc gatgcggctg ccgcagaact
      181 ttctgatctg ctgcaacaaa cacaggaaca attgctctat cttcaggagc taggcatcag
      241 cggcgtagag ccggcgacgt ctccgccaga actgccaaca caagccaacg agactctcga
      301 acagatctgg gccgaattga agggctgcac tcggtgtccg ctgcatgagg gtcgcacaca
      361 cgttgttaat acggagggaa atccccgagc gcgactgatg ttcgtcggcg aagctccggg
      421 agccgatgaa gactcgcaag cgcgcccgtt cgtcgggcgg gctggccaat tgctcacgaa
      481 gataatcgag gcgatcggtt tcaaacgcgg agacgttctg attggcaacg tcaatcgctg
      541 ccggccgcca gccaatcgtg ctcccctgcc cgacgaggcg gccacttgca agcctttcct
      601 gctgcgcgag attgccagcg tgaaacccga cgttatcgtc gttcttggca acacggcgat
      661 gaagaatttg cttgatacaa agatcggcat cacacggcta cgcggcgaat ttcaagattt
      721 caacggcatc aaagtgatgc ctacttttca tcctgcatat ctgttacgcg acccgtccaa
      781 gaagcgcgaa acctgggaag atctcaagaa ggttcgcgat tatcttgagc gcactgcaaa
      841 aagagcgaca tagactcagg acccagtcag cccctccaca cgctttctca tttcatcatt
      901 tcgcgttaac ttcttttcgt gccgcaaaag ttcggaagtt cacctcaagc gacagcgatt
      961 tcgcctgcgt ccagcgagca tgaattgaag gcgccccttt atgtggaggt ggcggtacct
     1021 cttcacgtct cgcaaacttt tacttaccgg ttgcccgaaa cggctgggga acttgcccaa
     1081 gtgggttcgc gggttctagt gccgctggga cggagtgccg ttacaggttt tatcgtcgag
     1141 ttgctgccgg acttaagctc gcatcggacc cttaccgatt ccgaaatcaa accggtcgaa
     1201 agagttctcg acgcaacgcc tttagttacg cccgaggtgc tagagatcac ccgctgggtc
     1261 tctcagtact atggctcgcc ctggggcgaa gttctgaagg gcgcattgcc gccgggaatt
     1321 aatccaaaca gcgaacagct tttcacgctg acggcgaacg gaagtgcacg gcttgccgac
     1381 ctcgaaagcg acgaggcaag ctctgaagca acggtcctgc gcgcgattgc agacgccggt
     1441 gaattgaatg tagaggcaat tgcaacgctc atcggaaagc gggaagcgat tcggacaacc
     1501 aaagagctgc agaatcggaa cttgattgtt attcaactca agtccgggca ggctagcgca
     1561 agaccaagaa ctcggcgagt tgcgaaactt cgacaacccc tcccaaccga agctctatcg
     1621 ccccgaaagc aaacggcggc gcaacaacga atactagacg ccctctcaaa tcaggaaacg
     1681 atgcctttgg cggagctgct tcgtatcacc ggcgtcagcc tctcggcggt aaacaccctc
     1741 cataagcagg gacagcttga gatactcgaa gagagtgttc ggcgagatcc cctcggaaaa
     1801 gtcccccttc cgcgcgcaga cgaataccaa ttgacgggag cgcaacgcca ggttctaagc
     1861 gagatagaac cgcaattgcg aagcggcgtt tacgccgcgt tcctgctaca cggcgtcact
     1921 ggtagtggca agaccgaggt ttacatgcgt gcaatgcgta tctgcctcga gctggggcgc
     1981 gcggcaatga tgttggtgcc cgagattgcg ctgactcctg tcttttcgcg ccggctgcga
     2041 tccaacttcg gggatcaggt cgcgatcttt cattcctcgc tctcgaaagg ggaaaggttt
     2101 gatgaatgga tgcgcgttaa gactgggcgc gcccggatag ttattggcac ccgctccggc
     2161 gtttttgctc ccatcgcaaa tctgggtctg gtgatagtcg atgaagaaca cgagtcaact
     2221 taccggcaac aggattctcc gcgctacaac ggtcgcgaca ctgccatcgt gcgcgcgcaa
     2281 aaagaaaacg cggttgtaat tcttggttca gccacccctt cgctggagtc gtttcacaac
     2341 gcccagaccg gcaagtacca ctatctgcaa ttaccctccc gcatcggcga tcggccgatg
     2401 gccgcagcac gcatcattga tatgcgcgaa gcatttgccc gtcataataa gccgcaagtt
     2461 ttttctgacg agttactgga agcaatcgag accacccatc agaaaggcga acagtcgatc
     2521 attctgctta accgccgcgg gtattcgagt tttgttctct gtcgttcctg cggcgagacg
     2581 attcaatgtc ccaactg
//
LOCUS       DASUEX010000312         3300 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1187638, whole genome shotgun sequence.
ACCESSION   DASUEX010000312 DASUEX010000000
VERSION     DASUEX010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3300
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1187638"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1048)
                     /locus_tag="VFV61_05255"
     CDS             complement(<1..1048)
                     /locus_tag="VFV61_05255"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HEX4899919.1"
                     /translation="MMLVLIADKFEQSGRDGLQALGCEVSFQPDLKDEALVAAIAETR
                     PDVLVVRSTKVTEPMLDVGSVKLVVRAGAGYNTIDVGAASKRGIYVSNCPGKNSIAVA
                     ELAFALILALDRRIADNVISLRRGEWNKKEFSKARGLFGRTLGLIGVGKIGQEMIPRA
                     TAFGMPVIAWSRSLTPERAEELGVGFRATPNEVAAEADIVSVHLALKPQTKGMIGAEF
                     LGSMRQGAYFINTARGDVVDQAALLEAMKTRGIRAGLDVFAAEPTSATGEFSDPIRNE
                     ASLYGTHHVGASTDQAQEAIAAETVRIIREFKETGKVPNVVNLALQTPATHRLVVRHQ
                     DRPGVLASVLDAIKA"
     gene            complement(1248..2474)
                     /locus_tag="VFV61_05260"
     CDS             complement(1248..2474)
                     /locus_tag="VFV61_05260"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HEX4899920.1"
                     /translation="MAVIHPFNALRPPPEKARQVSSVPYDVVNSEEAAALAVGNPLSF
                     LHVSRPEIDLPAGTDLYSDAVYRKALENFQKLIADCPLETEAVSSLYLYQLIMGDHEQ
                     VGLVACCSVDEYDRDLIRKHERTRRDKEDDRTRHMMVLQAQTGPVFLTYRARAEIDNM
                     VLETTMTKPLYDFTADDGIQHTIWHVPDAVRFVQAFRDVPYLYIADGHHRAASASRAR
                     AELQDKSFGHTGDEEYNFFLTVIFPDNQLQILPYNRVVKDLNGMSSDAFLAAVRKSFS
                     VAENGNPAPAQPQSWSMYLDGRWYELRLRNDAPPPEGLVNSLDVSVLQDRLLDTILGI
                     KDVRTDKRIDFVGGLRGTKELERLVNEGKAAVAFSLHATTVADLLRVSDAGEIMPPKS
                     TWFEPKLRDGLLIHKI"
     gene            2661..2867
                     /locus_tag="VFV61_05265"
     CDS             2661..2867
                     /locus_tag="VFV61_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899921.1"
                     /translation="MADRKSDRRSALEEKLRSVEETFAREMRQRGFEPDQAENVALPT
                     ALARLYTEREALKTELDELKGASE"
     gene            2864..>3300
                     /locus_tag="VFV61_05270"
     CDS             2864..>3300
                     /locus_tag="VFV61_05270"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX4899922.1"
                     /translation="MKETDRIRDQLKRAFAGGAWHGPGVLEILENVTASQAAAHPIAG
                     AHSIWELVLHIKAWEDACRRRLTGDRAELSDEEDWPPINETSDKAWSDILIGLREGHQ
                     RLSDAIASVDEARLNEPILEGMPSIYVTLHGVVQHDLYHAGQIA"
BASE COUNT          781 a          937 c          870 g          712 t
ORIGIN      
        1 cagccttgat cgcatccagc acactcgcca gaactcccgg tcgatcctgg tgacgcacta
       61 cgagtcgatg cgtagccgga gtctgcaaag ccagattcac aacattcggc accttcccgg
      121 tttctttgaa ctcgcgaata atacgcactg tttccgcggc gatcgcttcc tgcgcctgat
      181 cggtggaagc gccgacatga tgcgttccat acagacttgc ttcattgcga atcgggtcgc
      241 tgaattcgcc agtcgctgac gttggttctg cagcgaacac atcgagaccc gcgcgaatgc
      301 cgcgagtctt catcgcttca agcagcgcag cttgatcgac aacatcgcca cgagcggtat
      361 tgatgaagta ggccccttga cgcatcgacc caagaaactc cgcccctatc attcccttgg
      421 tttgcggttt taaagcgaga tgcacgctca caatgtctgc ttcagcggca acttcgttcg
      481 gcgtggcccg gaaaccgact ccgagttcct ccgcacgctc aggagtcagg ctgcggctcc
      541 aggcgatcac cggcatccca aaagcagtcg cgcgcggaat catctcctgc ccaatcttgc
      601 cgactccaat taagccaaga gtgcgaccaa acaatccgcg ggctttggag aactccttct
      661 tgttccattc accacgtcgc aaactgatca cgttgtcagc gatgcgccga tcaagcgcga
      721 gaatcagcgc aaaagccaat tccgcgacgg caatggagtt cttgccagga caattcgaaa
      781 cgtaaatgcc acgcttggaa gctgcaccaa cgtcaatcgt gttgtagccg gcgccagcgc
      841 gcaccaccag ttttaccgaa ccgacgtcga gcattggttc ggtaaccttg gttgagcgca
      901 ctaccagcac atcaggacga gtctccgcaa tagcggcaac cagcgcctcg tccttcaagt
      961 cgggctggaa ggagacctcg cagccgagcg cctgcaaacc gtcgcgtcca gattgttcaa
     1021 atttgtcagc gatcagtacg agcatcatct tatttccgat tgccaattgc cgcctgccga
     1081 ttgttcaaat tggtctttcc ttggatcttt ggtctttgat ctttgaagcc ttaacttttg
     1141 agcccgggcc tgtcgcagtt acgaattcaa gtgttgtaac ggctcacact aagaccaaag
     1201 gcctaagacc taagaccaat tggcaatcgc caatcgccaa tcggcaatta aattttgtgt
     1261 atcaacaatc cgtcccgcag ttttggttcg aaccaggttg atttgggcgg catgatttcc
     1321 ccagcgtcag aaacgcgcag tagatcagcc accgtcgtcg catgtaacga aaaggcgact
     1381 gccgcttttc cttcgtttac caggcgctcg agttctttgg tgccgcgcag gccgccgaca
     1441 aaatcgatac gcttatcggt gcgaacatct ttgatcccca aaatggtatc caataaacga
     1501 tcttgcaaga cggaaacatc aagtgaattc acgagccctt ccggtggcgg tgcgtcattc
     1561 ctcagccgca gttcgtacca gcgtccgtcg agatacatgc tccagctctg cggttgagcg
     1621 ggcgcgggat tgccgttttc ggctactgag aaactctttc tcactgctgc gagaaatgca
     1681 tctgaactca tgccattaag atccttcacc acgcgattgt aaggaaggat ctgcaactgg
     1741 ttatcaggaa agatcactgt gagaaagaag ttatattctt cgtcacctgt gtggccaaaa
     1801 cttttatctt gcagctcggc gcgcgcgcgg ctagcgctcg ctgcccgatg atgaccgtcg
     1861 gcaatgtaaa gatagggaac atcgcgaaac gcctgcacaa aacgaacggc atccggcacg
     1921 tgccagatgg tgtgttggat gccatcgtcg gcagtgaagt cgtaaagcgg cttcgtcatc
     1981 gtggtctcca gcaccatgtt gtcgatctcg gcacgcgcac gatacgtgag aaagaccgga
     2041 ccggtttgcg cctgcaatac catcatgtgg cgcgtccgat catcctcctt gtcgcggcgc
     2101 gtacgctcat gcttgcgaat caaatcccga tcgtactcat ctaccgagca acaagcgacc
     2161 agacctactt gttcgtggtc ccccataatc aactggtaaa gatacaaact ggaaaccgct
     2221 tcagtctcta aggggcagtc tgcaatcaac ttttgaaagt tctcgagtgc tttgcggtaa
     2281 acggcatcac tgtaaagatc agtgccggca ggtagatcga tctccgggcg cgacacgtgc
     2341 agaaaactca atggattacc gactgccagg gctgcagctt cttcgctatt caccacgtca
     2401 tagggaacgc tggaaacttg ccgggccttt tccgggggtg ggcgcaacgc attgaaagga
     2461 tgaatgaccg ccaccaaatt cctcctggac cagtcgggct gcaacactgc ggcttccaga
     2521 ccatgcgggc ccgattcttt ttttgacttg attgctacgg cttgaaacaa gactatccgg
     2581 tcaggcgcga tcttagcaag ccgcgacccg aaccgggttg aaggtgaggg atgaaggctg
     2641 aaagagtaag atatccgcta atggcagacc gtaagagcga tcggcgcagt gccctcgaag
     2701 agaagcttcg aagtgttgag gagaccttcg cgcgcgaaat gcgccagcga ggctttgaac
     2761 cggaccaggc tgagaacgtc gcattgccaa cggccctggc ccggctatat accgagcgcg
     2821 aagcactgaa aaccgaactg gacgagctga aaggtgcaag tgaatgaaag agactgacag
     2881 aattcgcgat caactaaagc gggcattcgc cggcggagcg tggcatggcc cgggcgtgct
     2941 ggagattctt gaaaacgtaa cggcaagtca ggcagccgcg catcccatcg ccggcgccca
     3001 cagcatttgg gaactcgtgc tccatattaa ggcgtgggaa gacgcctgcc ggcgtcgcct
     3061 aacaggagac cgcgcagaac tttccgacga ggaagattgg cccccaatta acgagaccag
     3121 cgacaaagcc tggagcgata tcttgattgg cttgcgggaa ggccatcaac ggttatctga
     3181 cgcaattgcc tccgtcgatg aagcgcggct taatgagccc attcttgaag gtatgccgtc
     3241 aatatatgta acgcttcacg gcgtggtgca acacgacctc tatcacgctg gtcagatcgc
//
LOCUS       DASUEX010000313         3011 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_981862, whole genome shotgun sequence.
ACCESSION   DASUEX010000313 DASUEX010000000
VERSION     DASUEX010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3011
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_981862"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..364
                     /locus_tag="VFV61_05275"
     CDS             77..364
                     /locus_tag="VFV61_05275"
                     /inference="COORDINATES: protein motif:HMM:NF015289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HEX4899923.1"
                     /translation="MILAKVIGNVVASQKNHRYENSRVMLCQQITPEGEETAMTVLAL
                     DAVDAGVGDTVLIVQEGWSASTTATGKAGAAIDSAIVGVVDYVDLLEKDLS"
     gene            361..780
                     /locus_tag="VFV61_05280"
     CDS             361..780
                     /locus_tag="VFV61_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899924.1"
                     /translation="MSTEREQAHQIAQRIAQRVSASQPATTPARATQNVTAELASVRA
                     VLSELQGKLAEIEAKFSAQPLTSGERSNPPRVPLTHSPWLAGVNASVSHPSQEKFGIE
                     EATVSELVDFFEKEKMCSVEPGGKPCDHCAMCSSRGF"
     gene            complement(850..1488)
                     /locus_tag="VFV61_05285"
     CDS             complement(850..1488)
                     /locus_tag="VFV61_05285"
                     /inference="COORDINATES: protein motif:HMM:NF015944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEX4899925.1"
                     /translation="MNLRRSLLTFFVVLMTLVGSGCGYNTLTTKQQNVKGKWANVETQ
                     LQRRSDLLNNLIETAKLAGVQEQEVFGKIAEARSRLLNASQQAPQGEGGDKSPEQKQA
                     VIDAANSFGGTIGRLLVLQEQYPQLRSNENFMKAQDEVVGTENRIATSRKDYNDAVQD
                     YNTTRARFPTVISAKVFGFKEEPYFKAEETATQVPRIDSNTLRQNQNSTPAK"
     gene            complement(1559..2311)
                     /locus_tag="VFV61_05290"
     CDS             complement(1559..2311)
                     /locus_tag="VFV61_05290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899926.1"
                     /translation="MAKRIVQEALNSLVQDLKATHGANLACVVLYGSAAAGDHIELRS
                     DYNLLIALNRITPEDLRQAQAPMREWQRLGHPLPVYFTVEELSDAADVFPIEFHQMAN
                     ARVVLYGPDPFASVHVSQQELRHQAEYELRSKLIQLRRLYIPASISIEKLCDLMSESL
                     ASFAALFRAVLVLHGKEAPVAKPDTVRATVELLKLEPEPFERIFEIRATGYQPATETE
                     ANDLFASYMLQLEQVIEAVDELHRQAEHQSKL"
     gene            complement(2387..>3011)
                     /locus_tag="VFV61_05295"
     CDS             complement(2387..>3011)
                     /locus_tag="VFV61_05295"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEX4899927.1"
                     /translation="LAVARGWGIGSKEGEKNGLLLVVAVKDRKYFTQISDHLEGDLPD
                     GLVGQIQREHLLPNFKRGDYSRGIYETIQSYVATLASKRGFSIDGIDQSYAYRGTPEP
                     ATSGRGNAGLPAGCCTILIVGFVILMLLSAASKGGRGGRGGGGSGWLGWLILSSLLSS
                     GSGRSSSSGWGGGGFGGGGFGGGGGGGFGGGFGGGGSFGGGGAGGGW"
BASE COUNT          652 a          885 c          788 g          686 t
ORIGIN      
        1 tgaccgagac tagagactca aaatccggaa cccgaaactc aaaactcgaa actcgaaact
       61 gagaactttc caccccatga ttctcgcaaa agtcatcggc aacgttgtgg catcgcagaa
      121 gaatcatcgc tacgagaatt cacgcgtgat gctgtgccag cagataacgc ctgaaggcga
      181 agagactgcc atgaccgtgc tggcgcttga tgcggtcgac gctggcgtcg gtgacaccgt
      241 tctcattgtg caggaaggtt ggagcgcatc gaccaccgcc accgggaagg cgggtgcagc
      301 catcgactcc gcgatcgtgg gcgtggttga ttatgtcgat ctgctggaaa aagatttgtc
      361 atgagcaccg aacgggaaca agcacatcaa attgcccaac gcattgcgca gcgtgtgtcg
      421 gccagccagc cggcaacaac tcccgcccgc gcgactcaaa acgtcacggc ggagttggct
      481 tctgtgcgcg cagtgcttag cgagttgcag ggaaagttag cggagattga agcgaagttc
      541 tcggctcaac ctctgaccag tggcgaaagg tcaaacccac cgcgagtacc gctgacccat
      601 tcaccgtggt tggctggtgt gaacgccagc gtgtcccatc ccagccagga aaagtttggc
      661 attgaagaag cgaccgtttc tgaactagtg gactttttcg agaaagagaa gatgtgcagc
      721 gtcgaacccg gcgggaagcc gtgtgatcat tgcgctatgt gtagttctcg tgggttctag
      781 ttttttcgct gagtgcgaag gcaaaaggcc gccctagtgg cggccttttc ttttggaggc
      841 aaagcccggt tactttgccg gggtgctgtt ctgattttga cggagcgtat ttgaatcgat
      901 gcgcggaacc tgcgttgcgg tctcttctgc cttgaaatac ggctcttctt tgaagccgaa
      961 aaccttggcc gagatgacgg tcgggaagcg cgccctggta gtgttgtagt cctgcaccgc
     1021 gtcgttgtag tccttgcggg aagtggctat gcggttctcg gttccaacca cctcatcctg
     1081 cgccttcatg aaattctcgt ttgaacgcag ctgcggatac tgctcctgca gaacgagcag
     1141 cctgccaatc gttccgccaa agctgtttgc ggcatcaatc acggcctgtt tttgttccgg
     1201 tgacttgtcg ccaccttcgc cctgcggcgc ttgctgtgac gcattcagca atcttgatcg
     1261 cgcttccgca attttaccga aaacttcctg ttcctgaacg ccggccaatt ttgccgtctc
     1321 aatcagattg ttgagcaggt ccgaacggcg ttgaagctgc gtttcgacat tggcccactt
     1381 gcctttcacg ttctgttgct tcgtcgtcag cgtgttatag ccacagccgc tgccgaccag
     1441 ggtcatcagc acaacaaaaa aagttagtag tgatcttctc aggttcattt gcaatctccc
     1501 ttgtggtact cgcctctgat ttggcccgct tctgacgagc cggttagcgc aggggttatc
     1561 aaagtttcga ctggtgttcg gcctgacgat gtaactcgtc caccgcttct atcacttgtt
     1621 caagctgcag catgtaggaa gcaaacagat cgtttgcttc cgtctcggtg gccggctggt
     1681 agcccgtcgc acgtatctca aaaattcttt caaaaggttc aggctcgagc ttcagcaact
     1741 caaccgtcgc gcgaaccgtg tcaggtttcg cgaccggggc ttccttgcca tgcagaacga
     1801 gaacggcgcg aaacaaggcc gcgaaactcg ccagactctc gctcatcaga tcacatagct
     1861 tctcgataga aatggaagcc ggaatgtaaa gacggcgcaa ctggatcaat ttgctgcgca
     1921 actcgtactc tgcctgatgg cgcaactctt gctgggacac atgtaccgaa gcaaaaggat
     1981 cgggaccata gagcactaca cgcgcgtttg ccatctgatg aaactcgatc ggaaatacgt
     2041 ccgcggcgtc agatagttcc tctaccgtga agtacaccgg caacggatgg cccagtcgct
     2101 gccactcgcg catcggcgcc tgggcctgcc gcagatcttc cggagtgatt cggttcaagg
     2161 cgatcagcag gttgtagtcc gaacgcaact cgatgtgatc accggctgcg gccgagccgt
     2221 aaaggacgac acaagccaga ttggctccat gcgtggcctt taggtcctgg accaggctgt
     2281 taagggcctc ctgcacaatt ctcttagcca tttttgtttt gctgatagta acagaaagtg
     2341 tcgcaaagct ggattagctt tggattgctg cgcgaaattt ctgctgctac caacctcctc
     2401 ctgcgccgcc gccgccaaaa ctgccgccgc caccaaatcc cccgccaaag cctccgcctc
     2461 cgccgccgcc aaagcctcca ccaccgaagc cgccgccacc ccagccactg cttgagcttc
     2521 tcccgctacc gctgctcaat aacgagctga gaatgagcca acctaaccaa ccactacctc
     2581 ctccgccccg accacctctg cctcccttac tagcagcgga gagcagcatg aggatgacaa
     2641 agccgacaat cagaattgtg cagcaccccg ccggcagtcc tgcgtttcct cttccactag
     2701 tcgctggttc tggcgtccca cggtaggcat aactctgatc gatgccatcg atgctgaacc
     2761 cgcgcttgct cgcaagcgtg gcgacatacg actgaatggt ttcgtagatg ccgcggctgt
     2821 aatcgcccct tttaaagtta ggcaacaagt gctcacgttg aatctggccc accagtccat
     2881 caggcagatc accttccagg tgatcactta tctgcgtgaa gtattttcgg tctttcactg
     2941 caacgacgag tagaaggccg ttcttctcgc cttctttcga gccaattccc catcctcgag
     3001 caaccgccag c
//
LOCUS       DASUEX010000314         2448 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_982130, whole genome shotgun sequence.
ACCESSION   DASUEX010000314 DASUEX010000000
VERSION     DASUEX010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_982130"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1956
                     /gene="dnaK"
                     /locus_tag="VFV61_05300"
     CDS             <1..1956
                     /gene="dnaK"
                     /locus_tag="VFV61_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEX4899928.1"
                     /translation="RPRPGFGENNKMSSKIIGIDLGTTNSVVAVMEGGEPTVIPNSEG
                     GRTTPSVVAFTKDGSRLVGQVAKRQAVTNPENTIYSIKRFMGRRFDEVTEEIKQVPYK
                     VQAAGGDVRIAAGGKEWSPPEISAIVLQKMKQSAEDYLGHKVDKAVITVPAYFNDSQR
                     QATKDAGKIAGLEVMRIVNEPTAAALAYGLDKKGDETIAVFDFGGGTFDVSILEVGEG
                     VVEVKSTNGDTHLGGDDIDERLIEWIIEEFKRDQGIDLSSDKMALQRLKESAEKAKIE
                     LSSTMETEINLPFVTADQSGPKHLAMKLTRSRFEQLAAPILQRLRGPVEQALKDAGLD
                     SKKIDEVVLVGGSTRIPKVQEIVRELFGKEPNKSVNPDEVVAIGAAVQAGVLSGEKTD
                     ILLLDVTPLSLGIETLGGVFTKLIERNTTIPTRKSEIFSTASDNQTSVEVHVLQGERQ
                     MAGDNRTLGKFHLVGIPPAPRGMPQVEVTFDIDANGIVNVSAKDMGTGREQKITITSS
                     SGLNKDEIDKMMRDAESHAADDAKKREEIEARNRLDGLVYQMEKTLNENREKAGSVAS
                     EVETAIAQGKTALQEGGVDKLNDAFNQLQAASHKLAEALYQQAPPPGAAPQAEADQAS
                     AAGAASGSGSGEDNVIDAEYVDVDENK"
     gene            2085..>2448
                     /locus_tag="VFV61_05305"
     CDS             2085..>2448
                     /locus_tag="VFV61_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013983884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEX4899929.1"
                     /translation="MAKQDYYQTLGVKRDAKPEEIKKAYRRLARKYHPDVNPGDKASE
                     ERFKLTTEAHDVLSDPKKRAVYDRFGQYSDNLADAAARGATPGSSSGGAAPGFDFTGF
                     DWGTSSGAGSGTSFKDIFS"
BASE COUNT          602 a          616 c          738 g          492 t
ORIGIN      
        1 agaccgagac cgggttttgg agagaataac aagatgagta gcaagattat tggtattgac
       61 ctgggaacta cgaactctgt tgtggccgtc atggaaggtg gcgagcccac agtcattccc
      121 aattcagagg gtgggcgcac gacgccttcc gtggtggctt tcaccaagga cggtagccgc
      181 ctcgttggcc aggtggccaa gcgccaggct gtcaccaacc ctgaaaacac tatttattca
      241 attaaacggt tcatgggccg ccgcttcgac gaagtgacgg aggaaatcaa gcaggttccc
      301 tacaaagttc aggctgcggg gggcgatgtc cgtattgccg cggggggcaa agagtggagc
      361 ccgccggaga tttccgcgat cgttttgcag aaaatgaagc agtcggcgga agactacctg
      421 ggtcataaag tcgacaaggc tgttatcacg gtgccggcct acttcaatga ctcgcagcgg
      481 caggccacga aagacgcagg caagattgcc ggtctggaag taatgcgcat cgttaacgaa
      541 ccgactgccg cggcgctcgc ttatggtctg gataagaagg gtgatgaaac aatcgccgtc
      601 ttcgactttg gaggtggcac tttcgacgtt tcaatccttg aagtgggcga aggcgtggtg
      661 gaagttaagt caaccaacgg tgacacccac ctgggtggtg acgacatcga tgaacgcctt
      721 atcgagtgga tcattgaaga gttcaaacgc gatcagggaa ttgatctttc gagcgacaag
      781 atggccttgc agcgtctgaa agagtcggcg gagaaagcaa agatcgaact ttccagcacg
      841 atggaaactg agatcaatct tcccttcgtt acggccgatc agtcgggacc caagcatttg
      901 gcgatgaaac tgacgcgctc acgcttcgaa caattggcgg cgcctatctt gcagcgcctg
      961 cgtggaccag tggagcaggc cctcaaggat gcgggtctgg attccaagaa gattgacgaa
     1021 gttgtactcg tgggtggctc tacacgcatc ccgaaagttc aggagatcgt tcgcgagttg
     1081 ttcggaaaag agccgaacaa gagcgtgaat cccgacgagg ttgtggccat cggcgctgcg
     1141 gttcaggccg gcgtgctgtc gggtgagaaa accgacatcc ttctactcga cgtaactccg
     1201 cttagtttgg gcatcgagac tctcggcgga gtgttcacga agttgatcga acgcaacacc
     1261 acgataccga cgcggaagtc ggagatcttt tctaccgcct ctgacaatca gacatcagtg
     1321 gaagtgcatg ttctccaggg tgagcggcag atggccggcg ataatcgcac cttaggcaag
     1381 ttccacctcg tgggcattcc gccggcacct cgtggcatgc cgcaggtcga ggtaaccttt
     1441 gatatcgatg cgaacggcat tgttaacgtt tccgcgaaag atatggggac gggtcgcgaa
     1501 cagaagataa cgatcacgtc atcttccggt ctcaacaagg atgagatcga caagatgatg
     1561 cgcgatgcag aatcgcatgc cgccgacgat gccaagaagc gtgaagaaat tgaagcgcgc
     1621 aatcggctcg acggtcttgt ctaccagatg gagaagactc tgaacgagaa ccgcgagaaa
     1681 gcgggcagtg tcgcttccga agtagagacg gccatcgccc agggcaaaac tgctttacag
     1741 gaaggcggcg ttgacaagct gaacgacgca ttcaatcagt tgcaggcagc ttcgcacaaa
     1801 ctagcggagg cactttacca gcaagcaccg ccgccgggcg ctgcccctca ggcagaggcc
     1861 gatcaagcgt cggcagccgg cgccgcttcg ggctcaggtt ccggcgagga caatgtcatc
     1921 gacgctgaat acgtcgatgt ggatgagaac aagtaaacac attgtgccgt tttagattgc
     1981 caagtgccga ttggtcagtg actcgaattc gcagctgaag tttaggctgg attagagaaa
     2041 ctgatcagtc ggcaatcggc agttttggtg ttggttacta ggcaatggcg aaacaggatt
     2101 actaccagac gctcggcgta aagcgcgatg ctaagcccga agaaatcaag aaggcttatc
     2161 gccggctggc gcgtaagtac catcctgacg taaatcctgg tgacaaggcg tccgaggaac
     2221 gtttcaagtt aacaaccgaa gctcatgacg ttctatccga cccgaaaaag cgggccgtct
     2281 acgatcgctt tggccagtac tccgacaatc ttgcggatgc ggcggcacgg ggagcgactc
     2341 caggcagcag cagcggcggt gctgctccgg ggttcgattt cacaggtttc gattggggca
     2401 ccagttccgg ggccggcagc ggtacaagtt ttaaagacat cttttccg
//
LOCUS       DASUEX010000315         4219 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_386321, whole genome shotgun sequence.
ACCESSION   DASUEX010000315 DASUEX010000000
VERSION     DASUEX010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4219
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386321"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..971)
                     /locus_tag="VFV61_05310"
     CDS             complement(15..971)
                     /locus_tag="VFV61_05310"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX4899930.1"
                     /translation="MKPKVTIAYGLVSLLLTLVTPVSGREVKRATANSQAALQALVDK
                     AARETIAKFADKKLEEKQLSITLIDLRDPARPQTASFRGNERVYPASVVKLFYLAATH
                     RLLEDKKITETPELKRAVKDMIVDSSNEATQYVLDVITHTTGGYELPPKEMEKWQYAR
                     NAVNRYFSSLGYTNINVNQKTFCEDAYGREQVSRGKNGENRNKLTTDATARLMMEVAT
                     GKAVTPARSQQMMELLKRDYAGTSKDGDDQGHGFTGIALKDIEGARLWSKAGWTSTTR
                     HDVAYIELLDGRKFVLAVFTTEHANEREIIPTVARLVIEGLK"
     gene            1206..2519
                     /locus_tag="VFV61_05315"
     CDS             1206..2519
                     /locus_tag="VFV61_05315"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX4899931.1"
                     /translation="MEKVSNEGLVRGIRQWDLIGLTINSVIGAGIFVLPAKAFGLIGP
                     YSLIAFVTCAFVAVLIVLCFAEVSSRFTDTGGPYRYARVAFGPTFGFQVGWLNWLARI
                     SAYATNCNLLVVYLSFFWPPASSGLFRALVITGLTATLTAINYVGVRDATVTSNIFTI
                     AKLLPLILFIAAGLFFLAPANFTLGTAPSLGSFSTTVLLIVYAFTGFENATVPSGEIE
                     NPRRSLPIAILAAIAIVTAFYLLIQVVCIGTLVNLGKTERPLAEAANSFLGPIGASLI
                     AAGAITSIIGNLNVNLLSSPRIPFAMALQGELPQGLAAVHKRFRTPHVAILLTAALVL
                     ALTLSSSLIYALTVSTIARLLAYAATCAALPRLRYQKDAPPAMFILPGGIVISIVAIL
                     LSLWLLSNSTLIEARDSAIAVGAGLAIYLLYRVFKKPDPTVEEPG"
     gene            complement(2906..>4219)
                     /locus_tag="VFV61_05320"
     CDS             complement(2906..>4219)
                     /locus_tag="VFV61_05320"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX4899932.1"
                     /translation="WITGGDWDHERWPETDGKAPLPTRELIDRFTPNTPVFVSRLDGH
                     MALANSLALKLAGITKDSRDPDGGLIVRDPKTGEPSGVLKDAAMSAVWRVIPASTFEE
                     KLAAVRAATEHAARLGVTSVQDMSAGADVGVYQTLLDRGELKTRIYAVAPLPAWERLA
                     RTGVRAHFGSDMLRIGGLKGFADGSLGSTTAFFYEPYLDAPTTRGLAGDEMFPEGAML
                     ERVRQADRAGLQVMIHAIGDRANDQILSLFAQVTGENGARDRRFRIEHAQHLRRQDIP
                     RFSSEKVIASMQPYHAIDDGRWAEKRIGHERAKTTYAFRSLLDAGATLAFGTDWTVAP
                     LDPMLSIYAAVARRTLDGKNPNGWIPEQKISVAEAVWAYTAGSAYAEFQEGSKGTLAP
                     GRLADIVVLSEDIFTIDPREIEKVKVVFTIMDGRIVYAAPGFSPS"
BASE COUNT          883 a         1186 c         1088 g         1062 t
ORIGIN      
        1 caggtaattt cacattactt caatccctca ataaccaacc gcgccactgt aggaatgatc
       61 tcgcgctcgt tcgcatgttc cgtagtaaag actgccagca caaacttcct tccatccagg
      121 agctcaatat aagccacatc gtgtctggta gtgctggtcc agccggcttt tgaccacaac
      181 cgcgcacctt caatgtcttt cagggcgatg ccggtaaatc cgtgaccttg atcatctcca
      241 tctttacttg tgccagcata atcgcgtttc aataactcca tcatctgctg gctccgtgct
      301 ggcgtgactg ccttaccggt agcgacctcc atcatcagac gcgcagttgc atcggttgtt
      361 agtttattgc gattctcgcc gttcttccca cgcgacactt gctcccggcc atatgcatct
      421 tcacaaaagg ttttctgatt gacattgatg tttgtataac caagagagct gaaatagcga
      481 ttaaccgcat tgcgagcgta ttgccatttc tccatctctt tcggcggcag ctcgtagccg
      541 cctgtcgtat gggtgatgac atcgagaacg tattgcgtcg cctcgtttga ggaatcgacg
      601 atcatgtcct tcactgcgcg tttgagttcc ggagtctcag tgatcttctt gtcctcaagt
      661 aggcggtgcg tcgcggcgag gtaaaagagt ttcacgacac tggccggata gactcgctcg
      721 ttaccacgaa agcttgccgt ctgaggacgc gcgggatcgc gcagatcgat gagcgtgatt
      781 gaaagctgct tctcttcgag cttcttgtcg gcaaattttg caatcgtttc acgagccgct
      841 ttgtcgacaa gggcttggag ggcagcttgc gaattagcgg tagcgcgttt tacctcgcga
      901 ccactcaccg gcgtgacaag tgtcagcagc agagaaacga gcccgtaggc aattgtgact
      961 tttggtttca tgagtgtgaa atgctgagcg gaagtgtgac gtcttattct caccgggctt
     1021 cagcccggtg agagttaaat accgattcca aggaaaccgt ctaaacggtt tcccttgcct
     1081 ggtcccaatt ggcaccgcgc taaagcgcgg tgagaatgag agtgattgtt tgtgcgcttc
     1141 ggtgcgaaat atctgaatca cacaatttgt tgtgctacta aacaatctag attataaggc
     1201 agctcatgga aaaagtatcg aatgaaggac tcgttcgcgg gatccgccaa tgggatctca
     1261 tcggactgac aatcaacagc gtgatcggtg ccggcatctt cgttttgccc gccaaggctt
     1321 ttggtctgat cggaccctac agcctgattg cctttgtgac ctgcgccttt gtcgctgttc
     1381 tgatcgttct ttgtttcgcg gaagtcagta gtcgcttcac tgacaccggc ggtccctatc
     1441 gttacgcgcg cgtagccttt ggacccacct tcggctttca ggttggctgg ctgaattggc
     1501 tggcacgtat cagcgcctat gcaaccaact gtaatctcct ggttgtctat ttgagtttct
     1561 tttggcctcc ggcatcttca ggattgtttc gtgccctcgt gattacagga ctaaccgcca
     1621 ccctgacagc catcaactat gtcggcgttc gtgatgcaac cgttacaagt aacatcttca
     1681 ccatcgcaaa actgctgccg ctcatactct tcatcgcggc ggggctcttt ttcctggctc
     1741 cggcaaattt cacattggga actgccccat ctctgggttc attttcgacc acggtgctgt
     1801 taattgttta cgcgtttact ggtttcgaga acgcgaccgt gccttcaggc gaaatcgaga
     1861 accctcgtcg cagtctgccc attgccatcc tcgccgccat agcgatcgtc acggcatttt
     1921 acctgttgat tcaagtggtc tgtattggaa cgttggtgaa ccttggcaag actgaacgtc
     1981 ctctggctga ggcggccaac tcgttcctcg gtccgatagg ggcgtcgtta atagcagcag
     2041 gtgcgataac gtcgatcatc ggcaatctaa acgtaaacct gttgtcgtct ccacgcattc
     2101 cttttgcaat ggccctgcag ggagagttgc cacaaggact cgccgctgtc cacaagcgct
     2161 tccgcacgcc ccacgtcgcc atactgttga ctgcggcgtt agttctcgcg ctgacacttt
     2221 caagtagttt gatttatgcg cttacggtga gcacgattgc gagactgctg gcctacgccg
     2281 cgacctgtgc agctctgccg cgactgcgtt accagaaaga cgcacccccg gctatgttca
     2341 tcctgcctgg aggaatagtg atcagtatag ttgcaattct gctatctctc tggctcctat
     2401 caaacagcac tctgattgaa gcacgcgatt cagcgatagc tgttggggct ggacttgcga
     2461 tttacttact ttatcgggtt ttcaaaaaac ctgaccctac cgttgaagaa cctggctagt
     2521 gctttctgtg tccgtgagga ttcatgatta acgaacgaac cggcctgcct caagagtcat
     2581 gggccatctc cgtttcggct ctgccgccgg atgtgaggca gtggctatgc cccgccggag
     2641 agtgccctta cacgtttggg ctatttccgt ggtgtggcct taggcccaat aagctgtaag
     2701 gattgagcaa aataagggca tagcccgcga atattctgac tgctctcggc gcggcgagcc
     2761 gcgcctcaca tccggcggca aagccgtaac ctgcgtctgg ggtgacttat gaggcaaagc
     2821 tgaacgaacc actaaatcac actaactgac acgaaccggc agatttgtgt atttcgtgtg
     2881 aattcgtggt tgtggttccg cctcctcagg agggcgaaaa accgggcgcc gcgtaaacta
     2941 tccggccgtc catgatggta aagaccacct ttactttctc gatctctcgc ggatcaattg
     3001 tgaaaatgtc ttctgagagc accacaatat cggcaagcct gccgggggca agcgttccct
     3061 tggatccttc ctgaaactcc gcataggccg acccagctgt gtaggcccac actgcctcag
     3121 caaccgaaat cttctgttca ggaatccagc cgtttgggtt tttgccgtcg agcgtgcgac
     3181 gcgcaaccgc ggcatagatg ctcaacatag gatctaaggg agctactgtc caatctgtgc
     3241 cgaatgcgag tgtggctccg gcatcgagca aggagcgaaa agcataagtg gtcttggcgc
     3301 gctcgtggcc aattctcttc tctgcccaac ggccatcatc gatcgcgtga taaggctgca
     3361 tggacgcaat tactttttca cttgagaaac gcgggatgtc ctgacgccgc aaatgttgcg
     3421 catgttcaat gcgaaagcga cggtcgcggg cgccattctc gcctgtcacc tgcgcgaaaa
     3481 gagaaagaat ctgatcgttg gcgcggtcgc cgatggcgtg aatcatcacc tgaagtccgg
     3541 ctcgatccgc ctgccgcaca cgttcgagca ttgctccttc cggaaacatc tcgtcgccag
     3601 cgagcccgcg agttgttggt gcatcaaggt agggttcgta aaagaatgcc gttgtcgaac
     3661 caagactgcc atctgcaaat cctttcagac cgccaatgcg caacatgtcg ctgccaaagt
     3721 gcgcccgcac accggtacgc gccaatctct cccaagccgg caacggtgcc acggcataga
     3781 ttcgcgtctt taactcaccc cgatcgagca gggtttggta cacgcccaca tccgccccgg
     3841 cggacatgtc ttgcacacta gtaacgccca atctcgctgc atgttcagtc gcggcccgca
     3901 ccgcagctaa cttctcttca aaggttgaag ctggaataac tctccacacc gcactcattg
     3961 ctgcgtcctt gagcacgccg ctgggctcac cagttttggg atctcgcacg atcagaccgc
     4021 cgtctggatc gcgagaatct tttgtgatcc cggcaagctt caatgcgagg ctgttggcaa
     4081 gtgccatgtg gccgtcgagc cgactgacga agactggtgt attcggggtg aagcgatcaa
     4141 tcaactctct tgtgggtaag ggagccttgc catctgtttc cggccagcgt tcgtggtccc
     4201 aatcgccacc ggtgatcca
//
LOCUS       DASUEX010000316         3451 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1303557, whole genome shotgun sequence.
ACCESSION   DASUEX010000316 DASUEX010000000
VERSION     DASUEX010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3451
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1303557"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(160..2709)
                     /locus_tag="VFV61_05325"
     CDS             complement(160..2709)
                     /locus_tag="VFV61_05325"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEX4899933.1"
                     /translation="MAAKHPGSKPKVYTAKPGGNPPGQSRSVLRRFWLPAVLLLSLAA
                     GGLTGIIAAYKLNYSRAAIEVAALATYRPSVVTRVYADDGETVIGEFALEKRIPLKYD
                     QIPPLVRNAILAVEDARFYEHVGIDPIRIVGAAWKNLTTDKVEGGSTLTQQLAKNLFL
                     SKEQTYQRKFNEWALALQIERYYTKNQIMELYANHTFLGANAYGIEAGAETYFGKQAK
                     DLTIGEAALLAGIPKAPSEYSPTANAAKAKERRDLVLDLMARHGFATQADVDAAKAKP
                     IQLADTAYYQSQPRSSAFDYPVEYVRQDLEDKYTTRVAQGGLSVYTTINVEAQKRAYE
                     VVRAGLRRYDRGRGWRSAYTMIPTAANNGAGPPTAHENANFKHPDWYGNEYQVGRFLM
                     GLIMKVDRARNEATVRFGNYTATVGVADMGWSGRQPAGEFKPGYLSEFEIKEVDDKNR
                     RLKVSASQVPAVQGAMMTLNAKTGEIVTMVGGYDFYSTSKFNNATQAYRQTGSCFKPF
                     VYTAAVEWGMTPDTTVSGAPISIGSWNPHNYDGSLGNGDLPLKTALAKSMNIPAVHLL
                     QTVGIQTGAQMVRRFGIKVPMAPYLSSALGATEVPLDQMVSAYSAFPNKGVRVEPHMV
                     RRVLDRDGAVLEEWEKTTYKVMNEYVALTMVSMMRGVVESGTATAAQSLGVQLAGKTG
                     TVNDHTDVWFIGYTPTYVTGVWMGYPGRKKDLGGNMTGGVGALPFFIDFMKTFLKDKP
                     KETFDKAPSMPEDMKELFRQRQRELAAERALVAASEEEDETDDSTDAPTDTQPKLEQV
                     TLPPPPREDAGAPAPQPKDDAAPRTDAPPATTRPREVDAQKKKGKKGEEEP"
     gene            2972..3363
                     /gene="ssrA"
                     /locus_tag="VFV61_05330"
     tmRNA           2972..3363
                     /gene="ssrA"
                     /locus_tag="VFV61_05330"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT          724 a          940 c          943 g          844 t
ORIGIN      
        1 gagcattcgg ctgttaaccg aagggttgta ggttcgagtc ctacctgcgg agccaaagtt
       61 tctgcacaat aaaaaggccg tcggactgaa gttcgatggc cttttcatac aagcagtctc
      121 aaccattcgg caatcgccaa tcggcaatcg ccaatcgatt taaggttctt cctcgccctt
      181 cttgcccttc ttcttctgcg catcgacttc gcgcgggcgc gtggttgctg gtggtgcatc
      241 agtacgagga gcagcgtcat cctttggctg cggcgcggga gcaccggcgt cctcgcgcgg
      301 cggaggtggc agcgtgacct gttcgagttt tggttgcgtg tcggttggtg cgtcagtgct
      361 gtcgtcggtc tcgtcttcct cttcactcgc tgcaaccaga gcgcgctcgg cggctagttc
      421 gcgctgcctt tgccggaaca gttccttcat gtcctccggc attgaaggcg ccttatcgaa
      481 ggtctctttc ggcttgtcct tcaggaatgt cttcatgaaa tcaatgaaga acggtagggc
      541 accgacgccg cccgtcatgt taccgccgag atctttcttc cgacccgggt agcccatcca
      601 gacacccgtt acataagtgg gtgtgtagcc aataaaccaa acgtccgtgt gatcgttaac
      661 ggtaccggtc tttcctgcga gctgcactcc gagagactgg gcagcggtag ctgtacctga
      721 ttcaactacg ccgcgcatca tcgaaaccat cgtcagcgcg acatattcat tcatgacttt
      781 gtaagtcgtc ttttcccact cctccagcac ggcgccgtca cgatccagca cgcgtcgcac
      841 catatgtggc tcaacccgca cgcctttgtt tggaaaggcg gagtacgctg aaaccatttg
      901 atccagaggt acttcggtcg cacctaaggc ggacgaaagg tacggcgcca tgggaacctt
      961 aatcccgaaa cgacgcacca tctgggctcc ggtttgaatt cccacggtct gcagcaggtg
     1021 gacagcggga atgttcattg attttgccag cgcggtcttt aaaggcaggt cgccattgcc
     1081 tagtgagcca tcgtagttat ggggattcca ggaaccaatg gagatgggcg cgccgcttac
     1141 agtcgtatcc ggtgtcatcc cccattcgac cgcggccgtg tagacgaaag gcttgaaaca
     1201 tgagccggtc tgacggtagg cctgcgtcgc gttgttgaat ttgctggtgc tgtagaagtc
     1261 gtagccgccc accatggtga cgatttcccc ggtcttggca ttaagcgtca tcatcgctcc
     1321 ctgcactgcc ggaacttgtg atgccgaaac tttcaaacga cgattcttat cgtcgacttc
     1381 tttgatttcg aattcggaaa gatacccggg tttgaattca ccagctggct gacgtcccga
     1441 ccaacccatg tcggcgacgc caacagtcgc cgtgtaattg ccaaagcgca cggtggcttc
     1501 attgcgagcg cgatcgacct tcatgatcag gcccatgaga aaccgcccca cctgatattc
     1561 gttgccgtac cagtcgggat gtttgaagtt cgcgttctcg tgcgccgttg gcgggccggc
     1621 gccgttattc gccgctgtcg gaatcatcgt gtaagccgaa cgccagccgc ggccgcggtc
     1681 gtaacgacgg aggccggcac gaacgacttc atatgctcgc ttctgcgctt ccacattaat
     1741 tgtcgtgtaa accgataggc cgccttgcgc tacgcgggtc gtgtacttgt cttcaagatc
     1801 ctgacgcaca tactcgactg gataatcgaa tgctgatgag cgtggttgcg actggtagta
     1861 ggcagtatcg gcgagttgaa tcggcttggc tttggctgcg tcaacatccg cctgagtcgc
     1921 gaaaccatgt cgggccataa gatcaagcac taagtcgcga cgctcttttg ctttggcagc
     1981 gttcgcggtc ggcgagtact cactcggggc cttcggaata ccggcgagca atgctgcttc
     2041 accaatggtc agatcttttg cctgctttcc gaaataggtt tccgctccgg cttcaatgcc
     2101 gtaggcgttc gcgccgagga aggtgtgatt ggcgtacaac tccatgattt ggttcttggt
     2161 gtagtaacgc tcgatctgaa gtgccagggc ccattcgttg aatttgcgct ggtaagtttg
     2221 ttcctttgaa agaaacaggt tcttcgcgag ctgctgggta agagttgaac ctccctcgac
     2281 tttatcagta gtcaaattct tccaggcagc accgacaatg cgaatcgggt caatgccgac
     2341 gtgttcataa aaccgagcat cctctacggc caggattgca tttctcacca ggggcggaat
     2401 ctgatcgtat ttcagcggaa ttctcttttc gagggcgaac tctccgatca ccgtctcgcc
     2461 atcgtcggcg tagacgcgcg tcactacact gggacgataa gtggcaagcg ccgcgacttc
     2521 aatcgccgct cgggagtagt tcagcttgta agcggcgatg attccggtaa gcccgcccgc
     2581 ggcgagcgag agcagcaaga ctgccggcaa ccagaatctt cgcaggacac tgcggctttg
     2641 acctggcggg tttccgcccg gcttggcggt atagactttt ggtttcgacc cgggatgttt
     2701 tgcagccata cgattacctc agtatttcat ggtgccttat gatgccaatt aggacaatgt
     2761 ggcttcccac agtgacgcgg ttctctaata tttgatgggg attataactt atctatcatc
     2821 cgccaataag cgacgctctc ggagggtgac aatgtagcgc aagcggtcag tttgcataac
     2881 gaaaaccaac agttggcgtt tcaaacatgt gcaaagtggc agatcataac ctgacatcta
     2941 ccgctcgatc acctatactc tcaatgcaaa cgggggcgac aggcttcgac aggggtactg
     3001 gcggtcgacc ggatgtcatg ccgggctact ctatgcagct cgttaaaaag cgtagggaaa
     3061 aaacaattgc caataacaat gaattggctc tcgctgccta attaggctcg agcgtctcac
     3121 cgcaagttgc ttatgcgcgc ggagggagat gtcactcaga taagctggcg cgaaccttcg
     3181 gtccgtgggt tcgcgcgaga ctaaagtcgg actagctcat tctgaggtct tgtcacttca
     3241 ctgactcggc aatgagcgaa gttttgtcgg gtgcaagccc ggcagaaaag agaagtgaat
     3301 aagcatgtag agtctttcga cgttgtctct tggatccgag ttcaattctc ggcgcctcca
     3361 ccaaaacaat tgccgattgg cgattgccaa ttgcccattg ggtcttgtac gaacgagcca
     3421 acaatcctcc aaattctctg ggaaaatttt t
//
LOCUS       DASUEX010000317         2982 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_983042, whole genome shotgun sequence.
ACCESSION   DASUEX010000317 DASUEX010000000
VERSION     DASUEX010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2982
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_983042"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..1304)
                     /locus_tag="VFV61_05335"
     CDS             complement(18..1304)
                     /locus_tag="VFV61_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HEX4899934.1"
                     /translation="MSSKLRFSATALTIFICGSCFANSFAQTQIQGQVLISELRLAGE
                     AGVEDEFVEIYNNTDSDIFVTATDQSAGWAVVVSFGQITGPLFIIPNGTRIPARGHFL
                     GANENGYSLSAYPSGNPTGTKVPFGITPGPFATAIPNATWAFDIPENDLTVGLALFST
                     SNTISQDLGNRLDAFGMAKAPTLFREGAGFPSAPFNRTLVRDMRTLTGIPKDKDDNAA
                     DFLLLSDGGRTGSVSAGELLGAPGPENLSSPVTNVHPLKATLLDPVAGSNGPPNRERR
                     PNVEPNANLGVLLIRRTIINKGSEPISRLRFRVVDVTGPGSENTCGGAVCADVRVLSS
                     QDGEAGSPNIGVVTVRGLTLEQPPVQANGGGFNSSISADSITLQTPLVPGASVNVEFK
                     LGVMRTGAFRLLVNYEAQNFSVPTQPGARSRQISKN"
     gene            complement(1507..2151)
                     /gene="can"
                     /locus_tag="VFV61_05340"
     CDS             complement(1507..2151)
                     /gene="can"
                     /locus_tag="VFV61_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HEX4899935.1"
                     /translation="MKNLNELLENNRAWAEGIKTRDPEFFQKLAQQQNPRYLWIGCSD
                     SRVPATQLVGLPPGEMFVHRNVANVVVHTDVNCLSVIQFAVDVLKVDHVIVCGHYGCG
                     GVQAAIDNLQLGLIDNWLRHVQDVMHNHVAILANITDAEQRLNRVCELNVIEQVLNVS
                     RTTIVQNAWARGQELAVHGWIYGIENGLLRDLDIRIANQLELQEAYEEAVRASG"
     gene            complement(2190..2870)
                     /locus_tag="VFV61_05345"
     CDS             complement(2190..2870)
                     /locus_tag="VFV61_05345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899936.1"
                     /translation="MKAIILKSLLLSLVVCFGTPAFGQATGTKPETVPDVIVTRELPL
                     DVGQLDGVNYVNNFFGLSLSIPGDWILVQGRNKEIAQASKKLLANEEAKQRAEFEKSI
                     EQSTILLSLTRVPSGAPNNASLLVIAERLSSPAIKNGVDALRTLEAMTKNTSFLVEFQ
                     SGIRSETINGVEFGVATIKTTSGTDSFMQKVYMVVKKGYGVEFFFTYKDPAHLATYDA
                     FMKTVKIK"
BASE COUNT          698 a          820 c          763 g          701 t
ORIGIN      
        1 taagcattcc cttccttcta gttcttactg atctgacgac tacgcgctcc gggctgggtc
       61 ggcaccgaaa agttttgcgc ttcatagtta accaggagac ggaatgctcc ggtgcgcatc
      121 acccccagtt tgaattcaac attaacgctt gctcccggta ccagcggcgt ctgcaacgtt
      181 atcgagtccg ccgatatgct cgaattgaaa ccgccgccgt tcgcttgcac tgggggttgc
      241 tccaacgtca atccccgcac cgtcacgacg ccgatgttcg gtgatccagc ctctccgtcc
      301 tgcgaactca acactcttac gtcagcgcat accgcgccgc cgcaggtgtt ctccgaaccg
      361 ggaccagtga cgtcgacaac tcggaatcgc aatcgcgaga ttggctcact ccctttgttt
      421 atgattgttc tccgaatcaa caaaacccca aggttagcat tcggctcaac gttcggtcgt
      481 cgctcacggt tgggcggacc attgctgccg gctaccggat ctaaaagtgt agctttcaag
      541 ggatggacgt ttgtcacggg actactgaga ttctccggac ccggagctcc gagaagttcg
      601 cccgcactca cgctgccggt gcggcccccg tcactgagta acagaaaatc ggcagcattg
      661 tcgtctttgt ctttcggtat gccggtcaga gtgcgcatgt cgcgcacgag tgttcgattg
      721 aacggcgcgc tgggaaagcc ggcgccttct cgaaacaatg tgggagcctt cgccattcca
      781 aacgcgtcga gacgattacc caggtcctgg gaaatagtgt tgctcgtgga gaaaagcgcc
      841 aaaccgactg tcagatcgtt ttccggaatg tcaaaagccc aggttgcatt tggaatcgca
      901 gtggcgaatg gccctggtgt aattccaaac ggaaccttag tgccggtcgg gttaccggaa
      961 ggataagcac tcaggctata tccattctcg ttagcaccaa gaaaatgtcc ccgcgcaggt
     1021 attctcgtgc cgttaggaat gataaaaagc ggcccggtta tctgaccgaa agacacaaca
     1081 accgcccatc cagcagactg atcagtggcc gtgacgaaga tgtcactatc cgtattgttg
     1141 tagatctcga cgaattcatc ttccacgccg gcttcgccag cgagccgcag ctcgctaatc
     1201 aacacctggc cttgaatctg tgtctgagcg aatgaatttg cgaaacagga cccgcagata
     1261 aaaatggtaa gcgcggttgc ggaaaagcgt agcttggaac tcatatgccc tccctgagtg
     1321 tcgcgattga actactaatc ggggatgaca gtgagacagt ccgctgacgg ctctaagaaa
     1381 tacaagggag cgggatattg aggagagcta tcccggaagt cgatgggttt tgcaagggct
     1441 caagatgaga attcaaagag aatgcgcaag gttttcagag gatctgactt catccagcag
     1501 cggcacctag ccggaagcgc ggactgcttc ttcataagct tcctgcaact ccaattgatt
     1561 ggcgatccgg atatcgagat cgcgcaacaa accgttttca atgccataga tccagccgtg
     1621 cactgcgagt tcctggccac gcgcccaggc attttgcaca atcgtggtgc gactgacatt
     1681 caatacctgt tcaatcacat taagctcaca gacgcgattc agtcgttgct ccgcatcagt
     1741 aatgttcgcc aggatcgcca catgattgtg catcacgtct tgaacgtgac gcagccagtt
     1801 gtcaatcaac cccaattgca aattatcaat tgccgcctga acgccaccgc agccgtagtg
     1861 gccgcaaacg atcacatgat caaccttaag cacatcaacc gcgaactgga tcaccgatag
     1921 gcaattgacg tctgtatgca cgaccacgtt tgccacattg cggtgcacga acatttcacc
     1981 gggcggcaag ccgacgagct gcgtagcggg tacgcggctg tcggagcaac caatccagag
     2041 gtaacgggga ttctgttgct gggccagctt ttgaaagaat tcgggatccc gtgtcttgat
     2101 gccttcggcc caggctcgat tgttctcgag tagttcgttt aagtttttca ttaaactgaa
     2161 gtgtcagggc agatgcgctg cagcgctcgt cacttaatct tcaccgtttt cataaacgca
     2221 tcatacgtgg ccaggtgcgc cgggtctttg taagtgaaaa agaattccac gccgtagccc
     2281 ttctttacca ccatataaac cttctgcatg aaactatccg ttcccgaagt tgtcttgatc
     2341 gtggcgacgc caaattctac cccgttgatg gtttcactac gaatgccgct ctggaactcc
     2401 accaaaaagc ttgtgttctt cgtcattgct tccagcgtgc gaagcgcatc cacgccgttc
     2461 ttgatcgccg gagagctgag acgctcggca atcacgagca acgaagcatt gtttggagcg
     2521 ccggagggta cacgggtcag gctcagcaaa atggtactct gctctatcga tttctcgaat
     2581 tcggcgcgct gcttcgcttc ttcgtttgcc agcagtttct ttgaggcttg ggcgatctct
     2641 ttattccgcc cctgaaccag gatccaatcg cccggtattg aaagcgaaag gccaaagaag
     2701 ttgtttacgt agttgacgcc atcgagctgg ccgacatcga gtggcagctc tcgagtaacg
     2761 atcacatcag gaacggtctc aggcttagtg cccgtggcct ggccgaacgc aggtgtcccg
     2821 aaacacacca ccagtgacag caataaggat tttaggataa tggctttcat gtaagatctc
     2881 cttaagatca acatggctct ctgagaagct cggctagctg caagtcaggt catcgatttt
     2941 atcagcgctc gtcaatctta ttgtgcagaa gatagtcttg cc
//
LOCUS       DASUEX010000318         4900 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1555736, whole genome shotgun sequence.
ACCESSION   DASUEX010000318 DASUEX010000000
VERSION     DASUEX010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4900
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1555736"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2722
                     /locus_tag="VFV61_05350"
     CDS             <1..2722
                     /locus_tag="VFV61_05350"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HEX4899937.1"
                     /translation="KEHVGVVQPPEAGTLKADEQALLDIFTQLRLRTGHDFSNYKRST
                     ILRRIERRMGLREIPGLPAYGNYIREHPPEVQALMKDLLISVTNFFRDPEAVKSLDSK
                     IIPLILEHKNPGESVRVWVAGCATGEEAYSLAMLFAEHIGAESTGTKLQVFATDLDEQ
                     SIRIARDGHYKDSEVADLSPVRLERFFHREVDGYRVRRELRESVLFAVHNVIKDPPFS
                     HLDLISCRNLLIYLNRLAQKRVLEVAHFALNPWGHLFLGASESIESANDLFTVVDKDH
                     HIYRSRPVPSRAVPVPEVSFKLREPPLTAREKFPDARALERLSYADLHQRLLEQYAPP
                     SIIINQEYDIVHLSERAGQFLQIIGGEPSQNLLRLARPGMRLELRTALHQAVHNATNV
                     EARGLKVATDQGIKTVNLIVRPVLGAEDSTSGLILVLFEETNPQQVSAPPSELAVADE
                     PLSRRLEDELVHLKGQLRATVEQYEIQQEELRASNEELQAMNEELRSAAEELETSKEE
                     LQSVNEELTTVNQELKIKIDEVSLANNNFQNLMNSTNIATIFLDRNLRVRQFTPTAKE
                     IFNLIPSDVGRPLLDITSKVKVDHLMSQMDTVMTHLETVEQEVKSLDDRSFMMRLSPY
                     RTLDNQVDGLVITLVDVTERARAAEIVRRVGEELEIKVRERTAELALSNEALRSEIQE
                     KERSEAARRRLLEQLVNAQEEERRRLARDLHDQLGQQLTALRLKLESLRAMKKGNAPA
                     TIDEIQRIVNQLDADVDFLAWEVRPIALDELGLEMALANYANQWSSHFNIAAEFHSRG
                     LDDNRLPPEIESNFYRIAQEALNNCAKHSQCQHADIIVERHHHHAVLIIEDDGVGFDT
                     QPEIQALRAMGILGMRERAVLMGGTFEIESTPGKGTTVFVRVPLSPAANV"
     gene            2727..3380
                     /locus_tag="VFV61_05355"
     CDS             2727..3380
                     /locus_tag="VFV61_05355"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX4899938.1"
                     /translation="MNKVRVLIAEDHHTVREGLKLILEAQPDIEVIGEAGDGRAAIEE
                     ARKLNPDIVLMDVSMPRLNGLKATQELKTCCPNVRVLTLTRHKDDGYLQQLLHAGADG
                     YVLKQSPPVELLHAIRSIAAGGRYLDPAVAGKVVGGYVAQTAPKGIVPKGELSERERQ
                     VLRLIAWGYSNKEIAAQLNLSVKTIEVHKANGMRKLGMSSRIDIVRYAILQGWLQET"
     gene            3799..4254
                     /locus_tag="VFV61_05360"
     CDS             3799..4254
                     /locus_tag="VFV61_05360"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX4899939.1"
                     /translation="MTGLPINERDPTAGTDLPQQPVVLVTDDHEDTRSLIRVFLTMRG
                     CLVIEAADGEEAVQIAETVRPDLILMDGFLPRLDGLGATRRIRALSDGHTPIVFICGR
                     AEPSFRARALEAGCDEYLLKPVSFDLLGSVLDRHLPQTTAINVPPAIDY"
     gene            4259..>4900
                     /locus_tag="VFV61_05365"
     CDS             4259..>4900
                     /locus_tag="VFV61_05365"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX4899940.1"
                     /translation="MKELLSNKILNRLPDADFERLLPLLEPISLSPRERLAEMGDTTR
                     FIYFPENSIISCQAEMQDGKSAEVGMVGKEGVAGLPPLFGPRPTVHSLSVAIAGNALR
                     LPTVVLEQELNRGNGLQETMTRYVFDYMTQVAQRAACAILHRMEQRFALWLLMVSDRL
                     GTNAVETTQERIAHHLGVRRAGITVVAGELQIIGAIAYRRGNLRIIDRTLLQKV"
BASE COUNT         1186 a         1278 c         1322 g         1114 t
ORIGIN      
        1 taaggagcat gtcggtgttg tgcaacctcc cgaagcggga actctcaagg ccgacgagca
       61 ggctttacta gatatcttca ctcaattgcg cctgcgcacc ggacacgact tttccaatta
      121 caaacgttca acgatcctga gacgaatcga gcgccgcatg ggcctgcgcg aaatccctgg
      181 actacctgcg tacggcaact acatccgcga gcaccccccg gaagtgcaag cgttgatgaa
      241 ggatttgctc atcagcgtga caaatttttt ccgcgatcct gaagctgtca aatcactaga
      301 cagcaaaatc attcccctga ttctcgaaca caagaatccg ggcgagtcag ttcgcgtttg
      361 ggtagcgggt tgtgctaccg gtgaggaagc ctattccctc gcgatgctct tcgcggaaca
      421 cattggggca gagagtacgg gtaccaaact gcaggttttt gctaccgacc ttgatgaaca
      481 atcaattcgc attgcccgcg acggtcacta caaggattca gaagttgccg acctctcgcc
      541 tgtacgattg gagcgtttct tccatcgcga ggtagatggc tatcgtgtcc ggcgggaact
      601 gcgtgagtcg gtattatttg cggttcacaa tgtgatcaaa gatccaccgt tttctcacct
      661 cgacttaatc tcctgccgca atctcctgat ctacctgaat cgcctggcgc agaaacgagt
      721 gttggaggtt gctcactttg cgctcaatcc ctggggccac cttttcctgg gtgcctcaga
      781 atcaattgaa agtgcgaacg acttgttcac cgtcgttgat aaagaccatc acatctatcg
      841 cagccggcca gtgccctcgc gtgcagttcc cgttcctgag gtctcgttca agttacgaga
      901 gccgccattg acagcaaggg agaagttccc tgacgcacgc gccctcgaac gactctcgta
      961 cgcagacctc catcagcgat tgcttgagca gtatgcgccg ccgagcatca taattaacca
     1021 agagtatgac atcgttcact tgtcagagcg cgcggggcag tttctgcaaa tcatcggtgg
     1081 cgaaccttca cagaatctgc tgcgcctcgc acgtccagga atgcgtctcg aattgcgcac
     1141 ggctctccat caggcagttc acaatgcgac caatgtggag gcccgtggtt tgaaggtcgc
     1201 aacggatcag gggattaaga ccgttaacct gattgtgcgc ccggttttgg gcgcagagga
     1261 ttccacgagt ggattgattc tggtcctctt cgaggaaacc aatcctcaac aggtatcagc
     1321 tcctcccagc gagctagcag tggcggatga accactatcg cgccgtctcg aggacgagct
     1381 ggttcacttg aagggacaac tgcgcgccac ggtggagcaa tatgagattc agcaggaaga
     1441 gctgcgtgct tcaaatgaag agctacaggc gatgaacgaa gaactgcggt ctgcggcgga
     1501 ggagctggag acgagcaagg aggaattaca gtccgtaaac gaggagttaa ccacagtcaa
     1561 ccaggaattg aagatcaaga ttgatgaagt cagtttggct aataataatt tccaaaacct
     1621 gatgaattct acaaacatcg caaccatttt cctcgaccgc aacctcaggg tcaggcagtt
     1681 cacgcccact gccaaggaga tctttaatct catcccctcg gatgtcggtc gtcctttgct
     1741 ggatatcact agtaaagtca aagtcgatca tctgatgtca caaatggata ctgttatgac
     1801 ccatctggaa acggtggaac aggaggtaaa gagtctggat gaccgatcat tcatgatgcg
     1861 gctttcgcct tatcgtactc tcgacaacca agttgacggt ctggtgatta cgctcgttga
     1921 cgttactgag cgggcgagag ctgcggaaat tgtgaggcga gttggcgagg aacttgagat
     1981 caaggttaga gagcgcaccg ccgagttggc cctatcaaat gaggccttgc gcagcgagat
     2041 tcaggagaag gagcgttcgg aagcagcgcg ccggagattg ttggaacaat tggtcaacgc
     2101 ccaggaagaa gagcgccgcc gactcgcacg agacctgcac gatcaactgg gacaacagtt
     2161 gacagcccta cgtctaaagc tggaatcgct gcgagcaatg aagaagggca acgctccggc
     2221 caccatcgat gagattcagc ggatcgtgaa ccaactcgat gcggacgtag actttttggc
     2281 ctgggaagtg cgaccaatcg ctcttgacga actgggtttg gaaatggcgc tggcgaatta
     2341 tgctaaccag tggtccagcc atttcaacat cgcggcagaa tttcattccc ggggactcga
     2401 cgataatcgt ttgccaccgg agattgaaag caatttttat cgcatcgctc aagaggcact
     2461 taacaattgt gccaagcatt cgcaatgtca acatgcggat ataattgtag agcgacacca
     2521 tcaccatgca gtgctaatca tcgaagacga tggcgtcggc ttcgatactc agcctgaaat
     2581 tcaagccctc agagccatgg gaatcctggg catgcgcgaa cgagctgttc tcatgggtgg
     2641 cacgttcgaa atcgaatcaa cacccggaaa aggcacaacc gtttttgtgc gtgtcccatt
     2701 aagtccggcg gctaacgtct gaagccatga ataaggttcg cgttctgatt gctgaggatc
     2761 accacactgt ccgtgaggga ctgaagctga tactggaagc acagcccgat attgaagtca
     2821 ttggcgaagc cggtgacggg cgagcggcaa ttgaagaagc gcgaaagttg aatcccgaca
     2881 ttgtcctcat ggacgtttcg atgcccagac tcaatgggct caaggctaca caggaactaa
     2941 agacatgttg ccccaacgta agagtgctaa cgctgacccg gcacaaagac gatggctact
     3001 tgcaacaact gctgcacgct ggggccgacg gatatgttct taaacaaagt ccaccagttg
     3061 aattgcttca tgcaatccgc tcgatcgctg ccggaggtcg gtatttagat ccggctgtcg
     3121 ccggtaaggt agtcggtggt tacgtcgctc agactgcccc caagggcatc gttccgaagg
     3181 gtgagttgag cgagcgcgaa cgccaggttc tgcgcttaat cgcctggggt tacagcaaca
     3241 aagagatcgc cgcgcaactc aacctaagcg tgaagactat cgaagtgcac aaggcaaatg
     3301 gaatgcggaa gctgggtatg agcagccgca tcgatatcgt ccgttacgcg attctgcaag
     3361 gctggttaca ggagacgtga agcatcttgc ttcgcgtggg ccaagtttgg tattcgactg
     3421 tacagacaaa atcatattgc ttttgtagtc tgctttcctg cgagactatc gcatctaaag
     3481 cgggtcaggg taactaaacc gcccttttgc cggaggacag gtttgaaacc ggcccgcttt
     3541 tgcattcacg aggaccgcta ctctgtcact ttgtccctgg ctccgcccac ctgattaggg
     3601 aaaatcctta ttccaaattc tcgctttcct gatagtccca tcggtcgcac cgtgcgataa
     3661 agtcaccctt atagggttga tagaaacgct aagctcagcc agctcggacg gtcgcgatga
     3721 cttctttagt cgcggtcaac gaatccgata gcggcactcg agcactctcc aggcattccc
     3781 tctacttaaa ctgaactcat gactggtctg ccgattaacg aacgagatcc cactgccggc
     3841 accgacttgc cgcaacagcc agttgtcctg gtgacagatg atcacgagga cacgcgatct
     3901 ctgatccgcg ttttcttgac catgcgcggt tgcctggtga ttgaagctgc ggacggcgaa
     3961 gaggccgttc agattgctga gaccgtacga ccggatctga tattgatgga tggcttctta
     4021 ccacgactcg atggtttggg ggccacgcgg cggatccgcg ccctgagcga tggccacact
     4081 cctatcgttt tcatttgtgg ccgagccgaa ccaagtttcc gtgcgcgtgc gttggaggca
     4141 ggctgtgatg aatatctact aaaaccggtg agctttgatt tactcggcag cgttttggac
     4201 agacacctac cgcaaacgac tgctatcaac gtacctccgg cgattgatta ctaagttaat
     4261 gaaagagctg ctcagcaata aaattctgaa tcgcttacca gatgcagact ttgagcgcct
     4321 gctgccgctg ctcgaaccta tctccctctc gcctcgtgag cggctcgccg aaatgggcga
     4381 caccacgcgg tttatctatt ttccagaaaa ctcaatcatc tcttgtcagg ccgaaatgca
     4441 ggacggaaag agcgcggagg ttggcatggt aggcaaggaa ggcgtagccg gcttgcctcc
     4501 gctgtttggt cctcgtccca cagttcattc cttatctgtg gccattgccg ggaacgcact
     4561 tcgtctgcca acggtagttt tggagcaaga gctaaatcgc ggtaatggtt tgcaagaaac
     4621 tatgacacgc tacgtgttcg attacatgac gcaggttgcg caacgggctg cttgtgcaat
     4681 tctgcatcgc atggaacagc gattcgctct ttggttgctg atggtcagcg atcgtctagg
     4741 tacaaatgct gttgagacta cgcaggaacg aatcgcgcat cacctggggg tacgtcgggc
     4801 gggaattacg gtcgttgccg gcgaattgca aatcattgga gccattgctt acaggcgggg
     4861 taacctgcgg ataattgatc gaacgctact tcagaaagtc
//
LOCUS       DASUEX010000319         3624 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1304064, whole genome shotgun sequence.
ACCESSION   DASUEX010000319 DASUEX010000000
VERSION     DASUEX010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3624
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1304064"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2170)
                     /gene="carB"
                     /locus_tag="VFV61_05370"
     CDS             complement(<1..2170)
                     /gene="carB"
                     /locus_tag="VFV61_05370"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF003671.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEX4899941.1"
                     /translation="MPKRADIHKILIIGSGPIVIGQACEFDYSGTQACKALRQEGYQV
                     VLVNSNPATIMTDPDTADVTYIEPLTVEAVSAIIRREKPDALLPTVGGQTALNLAMAL
                     AESGVLDEYNVEMIGAKREAVKVAEDRRLFKQAMDEVGLPMPRGGFARSWEDAREIVA
                     STAYPAIIRPSFTLGGTGGGTAYNPEEFEEIVRAGLAASPIHEVLIEESILGWKEFEL
                     EVMRDLADNVVVICSIENFDPMGVHTGDSITVAPAQTLTDVEYQRLRDMAITVIRKVG
                     VETGGSNIQFAVNPTNGDVRIIEMNPRVSRSSALASKATGFPIAKIAAKLAVGYTLDE
                     IPNDITKKTPASFEPTIDYVVAKIPKWEFEKFREAEDVLGTQMKSVGEVMAIGRTFKE
                     ALFKGLRSLEAVKPLRLEDVSDDELQRKLARPNSQRFSYITYALQHDWPIAEIYRLSG
                     VDPWFLEQLQEVMEIQREVEGKKLEEISPELLRVFKEAALSDRRLAYLTETTEDKVRS
                     FRQRLGVVPVYKRVDTCGAEFESFTPYLYSTYEQEDEAAPTNKRKIMILGSGPNRIGQ
                     GIEFDYCCCHASFVLRDEGFETIMVNCNPETVSTDYDTSDRLYFEPLTFEDVMNIVEV
                     EKPEGVIVQFGGQTPLNLAMRLHKAGVPIIGTTAESIDLAEDRKRFGSLLSELGIPQP
                     ESGMAATGAEAKEIAAKIGYPVLVRPSYVLGGRAMAIVYDE"
     gene            complement(2378..3175)
                     /locus_tag="VFV61_05375"
     CDS             complement(2378..3175)
                     /locus_tag="VFV61_05375"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX4899942.1"
                     /translation="MPAESSSKQAKNVIDEAAVCSGCFGTGVKIIAGKGAKICDDCQQ
                     RTGDSLLKAARIPPRYADCSFNTFRDPKGTGSIYWALMKAKMLVNEYPAVEKGLLLMG
                     PVGVGKTHLAVAILKGLTAKGVPGLFCEFGSLLKQIQDSYNPISQTSEMRVLAPVYQA
                     EILVLDELGATIPTDWVRDTMYQIINKRYNDRRLTVFTTNYLDEVRGEQPEPESATRS
                     FSRKASAERIKGMTTLEDRIGIRLRSRLYEMCNVVEIEGDDYRKTHR"
     gene            complement(3431..>3624)
                     /locus_tag="VFV61_05380"
     CDS             complement(3431..>3624)
                     /locus_tag="VFV61_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899943.1"
                     /translation="TVVFGVEVVIQKNRVVGVFTQELLCLLNILRHVNKIPLETYGKP
                     FVPTSIIVEKKNPDWIALD"
BASE COUNT          828 a          979 c          938 g          879 t
ORIGIN      
        1 cttcgtcata gacaatcgcc atcgctcggc cgccgagcac gtaactcggc cggaccagta
       61 ccggataacc aatctttgcg gcgatctctt tcgcttctgc gccggtggcg gccattccgc
      121 tttcgggttg tggaattccg agttcactta gcagtgaccc aaagcgtttg cgatcttcag
      181 cgaggtcgat cgactcggca gtggtgccaa taatgggcac gcccgccttg tgcagccgca
      241 tggcgagatt cagcggcgtc tggccaccga actggacaat cactccttcc ggcttctcta
      301 cctcgacgat gttcatcaca tcttcgaaag tgagcggctc gaaatagagt cgatcagaag
      361 tgtcgtaatc agttgagacg gtttcggggt tgcaattgac catgatcgtc tcaaagcctt
      421 cgtcgcgcag gacgaacgac gcgtggcagc aacaatagtc aaactcgatc ccctgaccga
      481 tgcgattcgg tcctgagccg aggatcatga tctttcgttt gtttgtaggc gctgcttcat
      541 cttcctgttc gtaagttgag tagagataag gcgtgaagga ttcgaattca gcaccgcaag
      601 tatctacacg cttgtaaacc ggcacaacgc caagtcgttg acggaaagat ctgactttgt
      661 cttcggtggt ttcagtcaga taagccaaac gtcgatcgga aagcgcggct tctttgaaga
      721 cgcgcagcag ctctggtgaa atctcctcga gctttttgcc ctcaacttca cgctgaatct
      781 ccattacctc ctgaagctgc tccaggaacc agggatcgac tcctgacagg cggtagattt
      841 ctgcaatcgg ccaatcgtgt tgcagggcat aagtaatgta ggaaaatcgc tgtgaattcg
      901 gccgcgctaa cttgcgctgc agttcatcat cagaaacatc ctcgagccgc agaggcttca
      961 ccgcttccag ggaacgcagc cctttgaaca aggcttcctt gaacgtgcgc ccgatggcca
     1021 ttacttcgcc aaccgacttc atttgcgtac ctagcacgtc ttcagcttcg cgaaactttt
     1081 caaactccca cttcggaatc ttggcgacta catagtcgat tgttggctcg aacgaagccg
     1141 gagtcttttt ggtgatgtca ttgggaattt catccagcgt gtaaccgacc gcgagtttgg
     1201 cagcgatttt ggcgatcgga aatccggtcg ccttggaggc gagggccgag gagcgcgaca
     1261 cgcgtggatt catttcgatg atgcgtacgt cgccgttagt cggattcaca gcgaactgaa
     1321 tattcgaacc gccggtctcg actcccacct tgcgaatcac cgtaattgcc atgtcgcgca
     1381 ggcgctggta ttcgacgtca gttagagttt gcgcgggcgc gacggtgatt gagtcgcccg
     1441 tatgaactcc catgggatca aagttctcaa tcgagcaaat cacaaccacg ttgtcggcaa
     1501 ggtcgcgcat cacttccagt tcaaactctt tccaaccaag aatcgattcc tcaatcagca
     1561 cttcgtgaat gggcgatgcg gcgagcccgg cgcgcacaat ctcttcaaat tcttccgggt
     1621 tgtaagcggt gcctcccccc gtgccgccga gcgtgaaaga tggacggatg atcgcggggt
     1681 aggcggtgct ggcaacgatt tccctggcat cttcccagga gcgcgcaaaa cccccgcgcg
     1741 gcatcggcag acctacttca tccatcgcct gcttgaagag gcgcctgtcc tccgcaactt
     1801 ttaccgcctc acgtttggcc ccgatcatct cgacgttgta ttcgtccagc acgcctgact
     1861 cggcaagcgc catggcgaga ttcagagctg tttgcccccc gacagtcggc agcaacgcat
     1921 ccggtttttc gcggcggata attgcgctca ctgcttccac tgtaaggggt tcgatgtaag
     1981 tgacgtcggc cgtgtcggga tcggtcatga tcgtcgctgg gtttgagttg accagcacga
     2041 cctgatagcc ctcctgacgc agagctttgc aggcctgagt tcctgaataa tcaaactcgc
     2101 aagcctggcc aatgactata ggaccggatc cgatgataag tattttgtgg atatcagcgc
     2161 gtttgggcat gcactacaga accggaggca ctcgctgccg cgctcgagta gccaattgct
     2221 cgaggcaacg aatgcaaaac ctttgaactt agtaatgtcg cgtcattata cataacccta
     2281 acggagcgca cgactcgacc ctgatccgga tattgctgaa ttgtgtagaa agcaaagaag
     2341 tgagcgaatg tacgtgaggg cgaaacactg caagccgtta gcggtgggtc tttcggtagt
     2401 cgtcgccttc gatttctacc acgttgcaca tttcgtacag acggctgcgc aagcgaatgc
     2461 cgatgcgatc ttcaagggtg gtcattccct taattcgctc ggcggacgct ttgcgactga
     2521 aagagcgggt tgccgactcc ggctccggtt gctcgccgcg aacttcgtcc aaataatttg
     2581 tagtaaaaac tgtcagccgc cggtcgttgt agcgcttgtt gatgatttga tacatcgtgt
     2641 cgcgcaccca atccgtggga atcgttgccc ctaattcgtc aagcaccaga atttctgcct
     2701 gataaacagg agccagaaca cgcatttccg aggtttgaga aatcgggttg taggaatcct
     2761 ggatctgctt gagcagcgaa ccgaactcgc aaaatagacc gggaacacct ttcgcggtga
     2821 gtcccttcag aattgccacg gctagatgcg ttttgccaac cccgaccgga cccatcaata
     2881 gcagtccttt ttcgacggct gggtattcat tgaccagcat cttcgccttc atcagtgccc
     2941 aatagatgga acctgttccc ttgggatcgc gaaaagtatt aaacgaacaa tcagcataac
     3001 ggggtggaat gcgagccgcc ttaagtaggc tatcgccggt gcgttgctga cagtcgtcgc
     3061 agatcttcgc gcccttgcca gcgatgattt tcaccccggt gccaaaacaa ccggaacaca
     3121 cggcggcctc atcgatgaca ttcttggctt gtttgctgga gctttcggca ggcattaagg
     3181 aattacggtt tctgtttcta ctttgcgagc aaattatagc attgcgaatc gataagacaa
     3241 cgaaggcgat tctccgcaag cgttaaatca tcaagatgca cctcgttgct ttgttggtat
     3301 atagttatta atccgatccc tcaacgaaac ctgatacaac gcaaaatgtc gttattgcgg
     3361 cggaggattt agtagcatta gcatgggcct gttagattca ataagaagca aacttggttt
     3421 aggcaaaaat tcaatccagc gcaatccaat ccgggttttt cttctcgacg atgatactcg
     3481 tcggcacaaa tggtttgccg tacgtttcaa gggggatttt attcacatgg cgaagaatgt
     3541 tgagcaggca caaaagctct tgagtgaaaa ctcctacgac gcgatttttc tggatcacga
     3601 cctccacccc gaacactaca gttc
//
LOCUS       DASUEX010000320         3177 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_632782, whole genome shotgun sequence.
ACCESSION   DASUEX010000320 DASUEX010000000
VERSION     DASUEX010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3177
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_632782"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            149..1057
                     /locus_tag="VFV61_05385"
     CDS             149..1057
                     /locus_tag="VFV61_05385"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX4899944.1"
                     /translation="MSELATKFPETHPMVGEISDHAAESTFAEVDDRNRETPAIEFRN
                     VHLSFDDRKILNNLSFKVMRGETKIILGGSGGGKSTIIKLVLGLLKPDAGRVLVDGED
                     ITNHSEVQMMTVRKKIGMVFQEGALFDSLSVYENVAYRLNEQGVAHDQIEPEVRRMLR
                     FVNLEDAIDKMPAELSGGMRRRVGIARALIGDPKIVLFDEPTAGLDPPTARTICELAM
                     KLRDLEDVSSIFVTHEMNNLTYLCSEYAVVNESGEVVFEREGERLCLINSKVMMMRQG
                     NIIFSGTDESLRNTEDPYIQKFLRGR"
     gene            1082..2227
                     /locus_tag="VFV61_05390"
     CDS             1082..2227
                     /locus_tag="VFV61_05390"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX4899945.1"
                     /translation="MPPSKKTVSWRELRVGLLVLGSIAILVFLILNASGDINPFSKKL
                     HLKARFADANGLREGSEVRLAGVRVGKIDKILLLTPSAEPNTPRVEAQMTINATIDGR
                     PANERIRTDSTAQQGSPSLLGNEMLINITPGTALGQPVADYAILPSSSSNTVNDFATS
                     GTDLAQRLSKLSDEINGMVRDVKDGKGTVGRLFSDEALYNNLNATIRETEDVMRQIRS
                     GNGSAGRFLNDPELYNNANTIANQLRLIAEDLRSGRGTAGKLLTDDQLYNRINRTADR
                     LDHSVDQINAMIVDINAGRGTLGKLIKDEQMYNDARAAIARFNTTAERIDNMVAAAQR
                     GEGTVGKLLTDEALYSNVNQLSSEGVKLIYDFRQNPKKYLTIKFELF"
     gene            complement(2255..2764)
                     /locus_tag="VFV61_05395"
     CDS             complement(2255..2764)
                     /locus_tag="VFV61_05395"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX4899946.1"
                     /translation="MAISDSILSEFDQEMAGTRRTLERVPAEKFDWKPHEKSTSMGGL
                     ATHLSNLPSWTGYTLEHESLDLAPGGQPLPGATIPASSEELLATFDKNVATARAVIAR
                     TSDQDFMKMWQLQRNGVTLLTLPKVAVLRGFVMNHNIHHRAQLGVYLRLNDIPVPSIY
                     GPSADESPF"
     gene            complement(2879..>3177)
                     /locus_tag="VFV61_05400"
     CDS             complement(2879..>3177)
                     /locus_tag="VFV61_05400"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX4899947.1"
                     /translation="QNHQLSENILESPGFQDITATVNWTQVKRVGLDAGLMTVELERQ
                     DRFLIREGLLEELEQRAIEAADNAEALRLRTSAREMILPSGMAASFQVLVQEKR"
BASE COUNT          744 a          858 c          870 g          705 t
ORIGIN      
        1 taccgacggg ggcaccgtag gtgtcgggcg cgcgactaca cgcgcagtcg tgctggcttc
       61 gatcgttgta atcattactg acttcttctt gtcacgcggt ctccaataca tactgggcat
      121 gccggtatcc taaaacgcaa aagcaatcat gtctgaactc gccacaaaat ttcctgaaac
      181 tcaccccatg gtcggggaga tcagcgatca cgccgccgag tcgacctttg ccgaggtaga
      241 cgatcgcaac cgggagacgc cggcaattga gtttcgtaac gtccatctct cttttgacga
      301 ccgaaagatc ctcaacaatc tcagtttcaa ggtcatgcgc ggtgaaacta agatcatcct
      361 gggaggttca ggaggcggca agtcgacgat tatcaaactc gtgctcggtt tgctcaaacc
      421 agatgcgggc cgtgtcctgg tagatggcga agatataacc aaccacagcg aagtgcagat
      481 gatgacggta cgtaagaaga ttgggatggt gtttcaggaa ggagcgctat tcgactcgct
      541 ctcggtttac gagaatgttg cctaccgcct caatgaacag ggcgtcgccc atgaccagat
      601 tgagccggag gtgcggcgca tgcttcggtt cgttaacctg gaagacgcga tcgacaagat
      661 gccggctgag ttatcaggcg gcatgcggcg ccgggtaggc attgcgcgcg ctttgatcgg
      721 cgatcccaag attgtcttgt ttgacgagcc gaccgcagga ctggatccgc ccacggcccg
      781 gacgatttgc gagctggcca tgaagttgcg agatttagag gatgtatctt cgatcttcgt
      841 tacgcacgag atgaataatc tgacttatct ctgttccgag tatgcggtgg ttaacgagtc
      901 tggagaagtc gtgttcgagc gtgagggcga acggctctgc ctgatcaaca gcaaggtgat
      961 gatgatgcgc cagggaaata ttattttcag cgggaccgat gagagcctgc gcaacactga
     1021 ggatccttac attcaaaagt ttctcagggg acggtaggat acgtcatttt aggagggcgt
     1081 catgccgcca tcaaagaaaa cagtttcgtg gagagagctt cgagtcggac tgttggtcct
     1141 gggctcgatc gcaatccttg tctttctgat tctcaatgcc tccggtgata tcaacccgtt
     1201 cagcaagaag ctgcacctta aggcccgctt tgcagatgcg aacggcctgc gagagggctc
     1261 ggaagtccgg cttgccggag tacgcgttgg gaaaatcgat aagatacttt tgctaactcc
     1321 ctcagccgaa ccgaacacgc ccagggtcga ggcgcagatg acgatcaacg caaccatcga
     1381 tggacgcccg gcgaacgagc gcattcgcac tgactctacg gcgcaacaag gttctcccag
     1441 cctgctcggc aacgagatgc tgataaacat tactccgggg actgctctgg ggcagccggt
     1501 agcggattac gcgatcctgc catcttcatc gtcgaacacc gtcaacgact ttgccacttc
     1561 aggcactgac ctggcccaga ggctcagcaa gctgtccgac gaaatcaacg gcatggttcg
     1621 cgatgtaaag gatggcaagg gaaccgtcgg gagactgttt tccgatgaag cgctgtacaa
     1681 caatctcaac gcaaccattc gtgagaccga ggacgtaatg cgtcagatcc gctcgggaaa
     1741 tggttcggca ggtagatttc ttaatgatcc cgagctctac aacaacgcga atacgattgc
     1801 caaccagttg cggctgattg ccgaggatct gcgcagcggg cgcggcactg ccggtaaact
     1861 gcttactgac gatcagctct acaaccgcat taatcgcacc gcggatcgtc tggatcactc
     1921 agtcgatcag atcaacgcga tgatcgtaga catcaacgcc gggcgcggta cgctcgggaa
     1981 gctaatcaaa gacgagcaga tgtataacga cgcgcgcgcc gcgatcgccc gcttcaacac
     2041 taccgctgag cgaattgata atatggtcgc cgcggcccag cgcggcgaag gaacggtggg
     2101 caaacttctc accgacgaag cgctctattc caacgtcaac caactttctt ccgaaggcgt
     2161 gaaactgatc tacgacttcc ggcaaaatcc gaagaagtac ctaaccatca agtttgaact
     2221 cttctaggat catccgacct aaaactgact agccttagaa cggactctcg tccgccgaag
     2281 gtccgtagat cgatggcacc ggaatgtcat tgagccgcag atagacgcca agctgtgcgc
     2341 ggtgatggat gttgtggttc atcacaaagc cacgcagcac ggctaccttt ggcagggtga
     2401 gcagcgtgac accattgcgc tggagctgcc acatcttcat gaagtcctga tcactggtgc
     2461 gggcgatgac agcgcgcgcg gtcgcaacgt tcttgtcgaa tgttgctagt agttcctcgg
     2521 aggaagccgg gatagttgcg ccgggaagtg gctgccctcc gggcgccaga tccaacgatt
     2581 cgtgttccag cgtgtagcct gtccaactcg gcaagttgct caaatgcgtc gccagaccgc
     2641 ccatcgacgt cgatttctca tgcggcttcc aatcgaactt ttccgccggg actcgctcca
     2701 gagtcctgcg cgtgccggcc atctcttgat caaattccga gagtattgaa tcgctgattg
     2761 ccattgggat cctcctcaaa cttgtgaatg atgttggttt gcgagaatag taaaaccagt
     2821 tcgtttgtgt ccaatgacgg gaatgcacgc actagcacga ttgattccca agtaagactt
     2881 aacgtttttc ttgcaccagc acttgaaaac tcgccgccat tccgctcggg agaatcatct
     2941 cgcgcgcact agttcttaag cgaagcgctt ctgcattgtc tgcggcttca atggcgcgct
     3001 gctcaagctc ttccagcaag ccttccctga ttagaaaacg gtcctgccgc tcaagttcaa
     3061 cggtcatgag tccagcatcc agtccaacgc gcttaacttg cgtccagttt actgtggcgg
     3121 tgatatcctg gaaaccaggg ctttcgagaa tgttttcgct caattgatga ttctgaa
//
LOCUS       DASUEX010000321         2212 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1555909, whole genome shotgun sequence.
ACCESSION   DASUEX010000321 DASUEX010000000
VERSION     DASUEX010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1555909"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2212)
                     /locus_tag="VFV61_05405"
     CDS             complement(<1..>2212)
                     /locus_tag="VFV61_05405"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX4899948.1"
                     /translation="LDHPHIARLLDGGTTDDGLPYFVMEYVEGEPLYRYCDKNQLTIA
                     DRLKLFRKACAAVHYAHQNFVIHRDLKPGNIMVSPEGVPKLLDFGIAKLLNPELAYHP
                     LDPTTAAMRLMTPEYASPEQVRGEKLTAASDVYALGVMLYELLTDHRPYRLKNHSPHE
                     LARVICEEEPELPSVAVNVIEVIGAEGEEPIETTPATISQARRTTPEQLKRELSGSLD
                     NIVLKALQKEVEKRYQSVEEFSEDIRRYLEGHPVSAPSFFPLSERTTLSNEEPTTLSR
                     SIAVLPFQVLRVEEKSEEFLGMGLADAIITKLSNIQRIIVRPTSAVVKYFDGTHNILA
                     AGQELNVGYVLDGRIQRAGDRIRVTVQLVTVRDGQPVWAAKFDENYTDIFAVEDSISE
                     QVAHALVPHLSGEERELLLRRETENANAYQAFLKGRYLWAKFTPDDFRLAVEQFREAI
                     RLDPNYAQAHVGVADYYNWAAIFGLGSPNENFPEAKSAAIRALEIDETLAEAHAALAF
                     TNLCYDWDWFGAEERFRRSIELNANYGPAHQWYSNLLAAQGRFEEAIAEIKHALEINP
                     LSLMDRSITGWTHYHARQYERAEQELLSALEIDRSFSNCYLMLGLVYERLGRYDDSLA
                     CLNKATELMSGSVVPFWAIGYTLAVSGKRDEAKAVIARLEGLSHQTYVSPYFLALVHT
                     ALGDHDLAFEFLDRAAENRDEWMIWLGVEPKLDPLRSDPRFALLLNRIGLKGDES"
BASE COUNT          510 a          603 c          592 g          507 t
ORIGIN      
        1 cactttcgtc gcccttcaaa ccgattcggt ttaacagtaa cgcaaaacgc ggatcggatc
       61 tcagcggatc cagcttcggc tcaaccccga gccaaatcat ccactcgtct ctgttctcag
      121 cagctctatc cagaaattca aacgcgagat cgtgatcgcc aagggcggtg tgaacaaggg
      181 caaggaagta aggtgagaca taggtctgat gagagaggcc ttcaagcctt gcaatgaccg
      241 ccttcgcttc atctcgtttt ccgctaacgg cgagggtgta accgatggcc caaaaaggca
      301 ccaccgaccc actcatcaat tccgtggctt tgttcagaca tgccagcgaa tcgtcgtaac
      361 gaccgagacg ttcgtaaacc agacccaaca tcaggtaaca gttgctgaag ctgcgatcaa
      421 tctccaatgc gctgagtagt tcctgttctg cccgctcata ctggcgtgcg tgatagtggg
      481 tccagccggt tatggaccgg tccatgagcg agagcggatt tatttccagc gcatgtttga
      541 tttcagcgat agcctcttcg aaacggcctt gcgcggcgag caaattggag taccactgat
      601 gagcgggtcc atagttggca ttgagctcaa tcgagcgacg aaagcgctcc tcagcaccga
      661 accaatccca gtcgtagcag aggttggtga acgccagtgc ggcatgagcc tcagcgagtg
      721 tttcatctat ctccagggcc cggattgcgg cactctttgc ctctgggaaa ttctcattcg
      781 gcgagccgag gccaaagatt gccgcccagt tgtaatagtc tgcgacgcca acatgggcct
      841 gcgcatagtt tgggtcgagg cgaatcgcct cgcgaaactg ttcaaccgct agccggaaat
      901 cgtctggcgt gaatttggcc cacagataac gacctttcag aaatgcctgg tatgcgtttg
      961 cgttctcagt ttcacgccgc agcagcaatt ctctttcttc gccggagaga tgtgggacga
     1021 gggcgtgagc gacctgctca gaaatcgaat cctcaacggc aaatatatcc gtgtaattct
     1081 cgtcaaactt agcggcccaa accggctggc catcgcgcac ggtaaccaac tgaacggtga
     1141 ctcgaatgcg atctccagca cgttggatgc ggccatccaa aacgtagccg acgttcaact
     1201 cctgaccggc agcaagaata ttgtgcgtgc cgtcaaagta tttgactacc gcgctggttg
     1261 gacggacgat gatgcgctgg atattgctga gctttgtgat gatcgcatcg gcgagaccca
     1321 tgccgagaaa ctcctcactc ttttcctcga cacgcaacac ctgaaatggc aagaccgcga
     1381 tcgatcgcga caaggtagtc ggttcctcgt tgcttaacgt cgttcgctca ctcaaaggga
     1441 aaaaagatgg ggcggaaacc gggtggccct cgagatagcg acggatgtct tccgaaaact
     1501 cctcaaccga ctggtaacgc ttctcaacct ctttctgcaa tgcctttaag acgatgttgt
     1561 ccaggctgcc tgacagttcg cgtttcaact gttccggagt ggtgcgacgt gcctggctga
     1621 tagtggccgg tgtggtctca atcggctctt cgccttccgc accaatcacc tcgatcacat
     1681 tcactgcgac gcttgggagt tccggctctt cttcgcaaat aacgcgcgcg agttcatgag
     1741 gtgaatggtt ctttaaccgg taaggacggt ggtcagtcag cagttcgtac agcatcacac
     1801 cgagcgcgta aacatcactg gcggcagtca gcttttcgcc gcgcacctgc tcaggactcg
     1861 cgtattccgg tgtcatcaac cgcattgctg cagtggttgg atccagtggg tgataggcaa
     1921 gttcgggatt gagcagcttt gcaatgccga aatcgagcag ctttggcact ccttccggag
     1981 aaaccatgat attgcccggc ttcaaatccc gatggatgac aaagttctgg tgagcatagt
     2041 ggaccgcggc gcaggctttg cgaaagagct ttaatcgatc ggcgatggtc agttgattct
     2101 tgtcgcaata gcgataaagt ggctcacctt caacatactc cattacaaaa tatggtaagc
     2161 cgtcgtcagt cgtgccaccg tcgagtaacc gagcgatgtg tggatgatcg ag
//
LOCUS       DASUEX010000322         7190 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1435228, whole genome shotgun sequence.
ACCESSION   DASUEX010000322 DASUEX010000000
VERSION     DASUEX010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7190
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1435228"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="VFV61_05410"
     CDS             complement(<1..320)
                     /locus_tag="VFV61_05410"
                     /inference="COORDINATES: protein motif:HMM:NF037651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899949.1"
                     /translation="MPWFSKKSKRIENVPPEERVVKTENVFVKCDGCEAHLYKRDLEE
                     SLQVCTHCGYHFRLGARERLGMLFDDGNFEELDAEVTSIDPLEFVDTKPYAERLKQAR
                     ESSG"
     gene            complement(427..1005)
                     /gene="cobO"
                     /locus_tag="VFV61_05415"
     CDS             complement(427..1005)
                     /gene="cobO"
                     /locus_tag="VFV61_05415"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF004637.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEX4899950.1"
                     /translation="MSTQEKKYKWRRADYQEQTRGLLMVHTGNGKGKTTCALGLLMRA
                     AGRGLNCCMIQFMKSRNDRYGEHESAEKLGIEVHTMGDGFTWDTKNPEQDRQTARETW
                     NLCVQKMRSGEYDLLVFDELVYVLSYQMLPIAEVLEEFRAIRAAQPALHIVVTGRDAP
                     PELIEAADLVTEMREIKHPFNVGIRAQQGIEF"
     gene            complement(1076..2065)
                     /locus_tag="VFV61_05420"
     CDS             complement(1076..2065)
                     /locus_tag="VFV61_05420"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEX4899951.1"
                     /translation="MLSQVVEVIESKRRFAITSHIRPDGDSLGSSLGLYWLLRALDKD
                     VEVIMRDPVPPAYRQLPGAETVRVTPAVDRPYHAVFVIECSDISRPGLIDLEQQLVVN
                     IDHHSTTALFGKINWIDSTASAVGEMIYNLCKATGVRVTKEIAECVYTALITDTGSFH
                     YSNTTERTFKVASELVRTGVRPAKTAEAVFASYPWSRIQLLGEVLTTARRDITGQVAC
                     MRLTHEMQERTAAPDEVTDGFVNYPLTVGEVEATALLKECEPGIYRTSLRSKGDVNVA
                     RVAEKFGGGGHRNAAGCTLKGDWDEVEREIIGLLQDAVQRANGAKDITEDGSQ"
     gene            complement(2155..2523)
                     /gene="rbfA"
                     /locus_tag="VFV61_05425"
     CDS             complement(2155..2523)
                     /gene="rbfA"
                     /locus_tag="VFV61_05425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEX4899952.1"
                     /translation="MRRPERVADILREEISQIVGYELEDPRLTMVTVTDVRLSDNLKA
                     ARVYFTVAGNEEEHRLALGALNHAAPYVRKQLGLSLNLPRTPEIHFVRDRVEEEGERV
                     DKLLDKIERDWAKEERMNDE"
     gene            complement(2614..5316)
                     /gene="infB"
                     /locus_tag="VFV61_05430"
     CDS             complement(2614..5316)
                     /gene="infB"
                     /locus_tag="VFV61_05430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEX4899953.1"
                     /translation="MTTSKIRIYDLAKELKLDTKRLIEEVRREGVDVSVPSNSVSKEL
                     AEKIRNKYFPKKETTVKRAIKVVKKAARPVEEAAAAEPEPPVVAEPEPLPEPVAAQPE
                     VEPTPAKAARPVVATKLLKKLPPATRAEAPELAESEAEPPLETEVVDDLDLAAALVAP
                     SATSPEPVKVPGASHQIRVMRPTAAALNAGIRPGERAPTPVLPPPVTPKERAERSGRV
                     RERNSLTRTERQGTPGETATPQTTYIPPPDAGRRRSRRAPVRGGRKGAEGKGGRFDKG
                     DFIPPPRTLSLEDRIAGRMDASVPGELKPARLVEGSTVKEFAEKLGIKPKDVVALLLQ
                     RGVFATINQPLNDDVATDLGKRFGYDVAFVPFEEMVAEEEFEELIATDADDVELPRAP
                     VVTVMGHVDHGKTSLLDGIRATDVAGGEAGGITQHIGAYSVQVPNPENKSETRRVVFL
                     DTPGHEAFTMMRARGAKATDIVVLVVAADDGVMPQTIEAIEHSRAAKVPIIVAINKID
                     KPDANPDRVKQELAQQGLSPVEWGGETEMVPVSAKKRQNIDTLLETILLSSDILNLRA
                     SPTRLASGVVLEAKLDRGRGPVATVLVQQGTLRTHDPFIVGQISGKVRAMFNDRGEAI
                     TEAIPATPVEVLGLQGVPQAGESFQVVADIGKAQQISQHRQMLSRQAALLQTTKRGIE
                     ALGESEVKELLVVIKADVQGSVEVLKSTLQKLSTERVKVKVIRSGVGAITESDVLLAS
                     ATQAGSSSSAVVIIGFNVRPESRAGELAKQEAVDIRLHSIIYKVEEEIRAAMIGMLEA
                     IEREKIIGKADVREVFKVPRVGAVAGSMVIDGNIRRNARARLIRDSVVTWEGNIISLR
                     RFKDDVSEVREGFECGIGLENFNDIKVGDQIEAYIVERVAATEL"
     gene            complement(5471..>7190)
                     /gene="nusA"
                     /locus_tag="VFV61_05435"
     CDS             complement(5471..>7190)
                     /gene="nusA"
                     /locus_tag="VFV61_05435"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEX4899954.1"
                     /translation="LCQEQGIERDLVIEAMKEAVRAAAKKQFRSGEDIQVDWNPETGI
                     ELSASKVVVEEVADAATELSIDEARELGGDEVEVGDALLLPLPMEELGRIAAQTAKQI
                     LFQKVRDAVRSNVYEQYIDKTGDLVNGYVKRFERGNIIVDLGNLEAILPRSQQSRGEQ
                     WNQGERIRVVINNVSKESKGPQVEVSRTSPELLLRLFEMEVPEIYDGTVVIKSAVREP
                     GERAKIAVTSNERDVDPVGACVGMKGSRVQAIIRELRGEKIDIIEWSDEPSVFAANAL
                     SPAKVNQVRITDIENRQMEVIVNEDQLSLAIGKRGQNVRLATKLVGWNIDIRSEEEIK
                     REVTEQMGALIASGASVPLSAIEGVTAQQTDTLAEHGINDIDALAHTSVDDLVEFLDV
                     SLDEAETILSAAKAVIAIRDTSMQGAGSETAEGDEVEADSEAAPGGDEPAVAVDETTA
                     GYDEAVATGTPFSAEPEILAQYSADPVAVTEAEPTSLEELILQEPGRDLRPDTITPAP
                     DITSTGAASIEYAEEVKEDGFTLEEVATLETSEVDNETAPGSEDAAADGSSDDEKQDA
                     SPDAAQ"
BASE COUNT         1437 a         2145 c         1925 g         1683 t
ORIGIN      
        1 attcccgaac tttcccgggc ttgtttcaag cgctctgcgt agggtttggt gtcgacaaac
       61 tccagcgggt caatcgaagt cacttcagcg tcgagctctt caaagttgcc atcgtcaaac
      121 agcattccca gccgttcgcg agcgccgaga cggaagtgat agccgcaatg cgtacaaacc
      181 tggagcgact cttcgagatc gcgcttatag aggtgtgctt cacagccgtc gcatttgacg
      241 aagacgtttt cggtcttcac cacgcgttct tccggtggca cattctcgat tcgcttactt
      301 tttttgctaa accagggcat gttgcgctgc tttaagcttt aaaacgttga ttattgtcag
      361 atgtggagcg gcaacgagat taccacatga gattaggaac gatgaaggac atgactcaaa
      421 gataacttag aactcaatgc cttgctgagc ccggattccg acgttaaagg gatgtttgat
      481 ttcgcgcatc tcggtaacga gatcggcggc ttcgattaac tcaggcgggg cgtcgcggcc
      541 ggtgacgacg atgtgtaacg cgggttgggc agcgcgaatc gcccgaaact cttccagcac
      601 ttctgcgatc ggcagcatct ggtaactcaa gacataaacc agctcatcaa aaaccagcag
      661 gtcatactcg ccactgcgca tcttctgtac acacaggttc caggtctcgc gcgcggtttg
      721 ccgatcctgt tccggattct tcgtgtccca ggtgaagcca tcgcccatcg tgtgtacttc
      781 gatgccgagc ttctccgccg actcatgttc accgtaacga tcattgcgcg acttcatgaa
      841 ttgaatcatg cagcagttta agccgcgccc ggcagcgcgc atcagcaacc cgagcgcaca
      901 agtcgttttg cccttgccgt ttccggtatg caccatcaac agcccgcgcg tctgctcctg
      961 gtaatccgcg cggcgccact tgtatttctt ttcttgagtg gacatgaagg aagtagacgg
     1021 cagtcagaag tcagaaggat tcaggtcgag gcctaagctg accactaaca actaactact
     1081 gactcccatc ttccgtgatg tctttcgcgc cattcgcccg ttgcaccgcg tcctggagca
     1141 agccgatgat ctcacgctcc acttcatccc agtcgccctt caaggtgcag ccggctgcat
     1201 tgcgatgacc gccgccgcca aatttctcag ccacccgcgc tacgttaaca tcacctttcg
     1261 aacgcaggct ggtgcgatag atacccggct cgcactcctt caacagcgcc gtcgcctcga
     1321 cttcacccac cgtcaacggg tagttaacaa aaccatctgt cacttcgtcc ggcgcggcgg
     1381 ttcgttcctg catctcgtgc gtcagtcgca tgcatgctac ctgacccgtg atatcgcggc
     1441 gcgcggtcgt cagaacttcg cccagcaact gaatccggct ccagggataa ctggcaaaca
     1501 ccgcctcagc agtttttgcc ggccttacgc cggtgcgcac cagctcagac gcgactttga
     1561 aagtgcgctc cgtagtattc gaataatgaa acgaacccgt gtcggtgatc agcgccgtat
     1621 agacacactc agcgatctct ttagtaaccc gcacaccggt ggctttgcac aggttgtaga
     1681 tcatttcgcc gaccgccgaa gcggtggagt cgatccagtt gatcttgcca aacagcgcgg
     1741 tcgtggaatg atggtcgata ttgacaacta actgttgctc gagatcaatc aagccgggcc
     1801 ggctgatgtc ggagcattcg atgacaaata ccgcatggta gggacgatcg actgcggggg
     1861 tgacgcgcac cgtttcagct cccggaagtt gccggtaggc tggcggaacc gggtcgcgca
     1921 tgattacttc aacgtctttg tcgagagcgc gcaacagcca gtaaagaccg agtgacgatc
     1981 ccagactgtc accgtcgggg cgaatgtgag aggtgattgc gaaacgacgt ttcgattcga
     2041 tcacctcaac tacttgacta agcatcttta cagcgatgag ggatgaggga tgaaaaacgg
     2101 agacaagtgc agcgcttttt taggcccacc tcgtcgccgt gtctcaacca ttcatcattc
     2161 atcattcatc ctttcctctt tggcccaatc tcgttcaatt ttgtccagca gtttgtctac
     2221 tcgctcgcct tcttcttcga ctcgatcgcg cacgaaatga atctccggag tgcgcggcag
     2281 gttaagcgag aggccaagct gtttgcggac ataaggcgcg gcgtgattca aagcgccaag
     2341 agccaggcgg tgttcctctt catttccggc aacggtgaaa tacacgcgtg ccgctttaag
     2401 attatctgaa aggcgaacgt cggtcacagt aaccattgtg agccgcggat cctccagttc
     2461 gtatccgact atctgtgaaa tctcctcgcg gagaatgtct gcaactcttt ccgggcgacg
     2521 cataacaatt aagggtgtcg gggaccggtg tcagataacg gattgtctct aatcaagtgc
     2581 ctgacatccg acacctaaca cccaacacca ttgttaaagc tccgtggcgg cgactctttc
     2641 gacgatgtaa gcctcgatct gatcgccgac cttgatgtcg ttaaagttct ccaggccgat
     2701 accgcactcg aagccctcgc gcacctccga aacgtcgtcc ttgaaacggc gtagggaaat
     2761 gatgttgcct tcccaggtga cgacgctgtc acgaatcagg cgggcgcggg cgttacggcg
     2821 aatgttgcca tcgatcacca tcgacccggc aaccgcaccg acacgcggca ccttgaatac
     2881 ttcgcgaaca tcggcttttc ctatgatctt ctcgcgctcg atggcttcga gcatgccgat
     2941 catcgccgcc cgaatctctt cttcaacctt gtaaatgatc gagtggaggc ggatatccac
     3001 tgcttcctgc ttcgccagtt ctccggcgcg tgactccggt ctcacattga aaccaataat
     3061 caccacagcc gacgaggatg acccggcctg cgttgctgaa gccaacaaca cgtccgactc
     3121 cgtgattgcg cccactccgg agcgaatcac tttcactttc acccgctcgg tggacagttt
     3181 ctgtagcgtt gatttgagaa cctctactga accctgcacg tcagccttga tgacgaccag
     3241 cagttcttta acttcactct cgccgagggc ttcgataccg cgcttcgttg tttgcagcaa
     3301 ggccgcctga cgactgagca tctggcggtg ttgcgagatc tgctgagcct taccgatgtc
     3361 ggcgactacc tggaaactct ctccggcctg cgggacacct tgtagcccga gtacttcaac
     3421 cggagttgca gggatcgctt ccgtaatagc ttcaccgcga tcattgaaca tcgcacgcac
     3481 tttgccggag atctgtccca cgatgaatgg gtcgtgtgtc cgcagcgtgc cctgttgcac
     3541 gagtacggtc gccaccgggc cacggccgcg atcgagtttt gcttccaaca ccacaccgga
     3601 ggccaaacga gttgggctcg ctcgcagatt caaaatgtct gagctaagca gaatggtttc
     3661 gagaagcgtg tcgatgttct ggcgcttctt cgctgaaacg ggaaccatct ctgtctcacc
     3721 accccactcg accggcgaca atccttgctg agctagttcc tgcttcaccc gatcaggatt
     3781 agcgtcgggc ttgtcgatct tgttgatggc cacgatgatt ggcaccttcg cagcccggga
     3841 gtgttcaatc gcttcgatcg tctggggcat aaccccgtcg tcggccgcca cgacaagcac
     3901 gacgatgtcg gtcgctttcg ctccgcgcgc gcgcatcatg gtgaatgcct cgtgaccggg
     3961 cgtgtcgaga aatacaacac ggcgggtttc cgacttgttt tcaggattcg gcacctgaac
     4021 gctataggcg ccgatgtgtt gcgtgatacc accagcttcg ccaccggcta catccgtagc
     4081 gcggatcccg tccagcaaag aagtcttgcc gtgatctacg tgacccatga cggttactac
     4141 cggagctcgc ggcagttcaa cgtcgtcggc gtcggtggca atcagttctt caaactcttc
     4201 ttcagcaacc atctcctcga agggcacaaa agccacgtca taaccgaaac gtttgcctaa
     4261 atcagtggca acgtcatcgt tcagtggttg gttgatggtg gcgaagaccc cacgttgcag
     4321 caggagggcg acgacatcct taggtttgat tcctaacttc tcggcgaatt cttttaccgt
     4381 cgaaccttca accagacgag caggcttcag ctcaccgggg actgaggcat ccatgcggcc
     4441 cgcgatacgg tcttcaagcg atagcgtacg cggcggcgga atgaagtcgc ctttatcgaa
     4501 acggccgcct ttgccttctg ctcccttgcg cccaccacga accggggcgc gccgcgagcg
     4561 gcgtcgacca gcgtctggcg gcgggatgta agtagtctgg ggagtggctg tttcgccggg
     4621 agttccctga cgttccgttc gcgtaagcga gttgcgttct ctgacgcgac cgctacgctc
     4681 agcgcgctcc ttgggcgtaa ccggcggcgg caacacgggc gtgggggcac gttcgccagg
     4741 acgaatgcca gcgttgagcg ccgcggcggt cggccgcatc actctaatct gatgagaggc
     4801 acccgggact ttcacgggtt cgggggaagt agcggaggga gctacgaggg ccgccgccaa
     4861 atccaaatcg tcaacaactt cggtttccag tggcggctcg gcttcactct cagcaagctc
     4921 tggagcttcc gctcgagtcg cgggcggtag cttcttgagc aacttcgtag caacgacggg
     4981 ccgggctgct tttgccgggg ttggttcaac ctcaggctgt gcggcgacgg gttctggcag
     5041 cggttccggt tccgcgacca cgggaggctc gggttcggca gcagctgctt cttcaactgg
     5101 gcgtgctgct ttcttaacga ccttgatcgc ccgctttact gtggtttcct ttttcgggaa
     5161 atacttattg cggatctttt cggccaactc tttggaaaca gagttggagg gaacgctaac
     5221 gtcgactccc tcgcggcgga cctcttcgat cagccgtttg gtgtcgagct tcagctcctt
     5281 tgccaggtca taaatgcgga ttttggaggt ggtcatgcac tccttctcag acgcttcagg
     5341 cgtcccgact cttcgtacgt gattaagaaa ctctcgctct taaccacctc gaagcgtgcg
     5401 tcagaagtgc aacgcggggt ggtggagcga gcagcgccac acgctggctg aaccagcgta
     5461 agcgcctgct ttactgtgct gcgtctggcg aagcatcttg cttctcgtcg tcactgctcc
     5521 catccgctgc agcatcttcg cttccggggg ctgtttcgtt gtcaacttca gaagtttcga
     5581 gagtggccac ttcttcaagt gtgaaaccgt cctctttaac ttcctctgcg tattcgatcg
     5641 aagccgcgcc cgtcgaggtg atgtccggcg cgggggtaat cgtatccgga cgcaggtctc
     5701 ggccgggttc ctgcaggatc agctcttcaa gggaagtcgg ttcggcttca gttactgcca
     5761 ccggatcggc tgaatactgg gccaggattt ccggctcggc agaaaacggt gttccggttg
     5821 cgacggcctc gtcataacct gccgtagttt catccacggc gaccgcaggt tcatcaccgc
     5881 ctggcgcagc ttcggagtcc gcttccactt cgtcaccttc cgcagtttca ctacctgcgc
     5941 cctgcatgct cgtatcgcga atcgcgatca cggctttcgc tgcgctcaag atggtctcgg
     6001 cctcatccaa actaacgtca agaaactcga ccaggtcatc gactgaagta tgggcgagcg
     6061 cgtcgatgtc attaatgccg tgttccgcaa gcgtgtcggt ttgctgggcc gtgactcctt
     6121 cgatcgcgga caaaggcaca gaagctcccg atgcaatcaa ggcacccatt tgctctgtga
     6181 cttcgcgttt gatttcttcc tcgctgcgaa tgtcaatgtt ccaaccaacc agcttcgtcg
     6241 ccaggcgcac gttttgcccg cgcttgccga tagcgagcga gagttgatcc tcgttcacaa
     6301 tcacttccat ctggcgattc tcgatgtcgg taatgcgcac ctgattcacc ttggccggtg
     6361 acaaagcatt agcggcgaat actgagggct cgtccgacca ttcaataatg tcgatctttt
     6421 ccccacgcag ctcgcgaatg attgcctgca cgcgcgaacc cttcatgccc acgcacgcgc
     6481 ccactggatc cacgtcgcgc tcattcgaag tgacggcgat cttcgcgcgt tcacccggct
     6541 cgcgcacggc cgatttgatc acaactgtgc cgtcgtagat ctccggaact tccatctcaa
     6601 acaaacggag caataactcc ggtgaggtgc gcgagacttc aacctgcggg cctttagact
     6661 cttttgagac gttgttaatc acgacccgga ttctctcgcc ctgattccat tgctcgcctc
     6721 gcgactgttg cgagcgcggc agaatcgctt ccagatttcc taaatcaacg ataatgttgc
     6781 cgcgttcgaa acgcttcacg tagccgttaa ccagatcacc cgtcttgtcg atgtactgtt
     6841 cgtagacatt gctacgtacg gcgtccctga ctttctgaaa aagaatctgt ttcgctgttt
     6901 gcgcggcgat ccttccgagt tcttccatcg ggagcggcag caaaagagcg tcgccgactt
     6961 caacttcatc gccgccgagt tcgcgagctt catcaatgga cagctcggtg gcggcatcag
     7021 ctacctcttc gacgacgact ttggaagctg agagctcgat gcccgtttcc ggattccagt
     7081 cgacctggat gtcttcgccg gagcggaact gtttcttggc agccgcgcgc accgcctctt
     7141 tcatggcttc gatgacgagg tcgcgttcga tcccctgctc ctggcaaagg
//
LOCUS       DASUEX010000323         2743 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1556644, whole genome shotgun sequence.
ACCESSION   DASUEX010000323 DASUEX010000000
VERSION     DASUEX010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2743
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1556644"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="VFV61_05440"
     CDS             complement(<1..292)
                     /locus_tag="VFV61_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899955.1"
                     /translation="MKKALVVSLGLGLVNLGAHPVPGQSQIEPGAAPRVVTESGEPLT
                     LSEAVEIALAKNPLTRGTAAGRQFADAQLAAARATRLPLLQASESVTTSNNPV"
     gene            complement(317..538)
                     /locus_tag="VFV61_05445"
     CDS             complement(317..538)
                     /locus_tag="VFV61_05445"
                     /inference="COORDINATES: protein motif:HMM:NF022577.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HEX4899956.1"
                     /translation="MTIERYLRLIAGCFVLATLLLGYFHSPYWFLFTGFVAINLIQSA
                     FTNWCPMISFLRWLGAKSEAGQFSSRSEA"
     gene            923..1261
                     /locus_tag="VFV61_05450"
     CDS             923..1261
                     /locus_tag="VFV61_05450"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX4899957.1"
                     /translation="MTVNKVVTKIEKEIFERQARICKAFAHPARLEILDLLGQGERGG
                     SELQEALGISKTRMSQHVSILKSAGVLTTRREGKQLYCSLAMPEVKQACQLIRKVLQG
                     QIAASSHLVP"
     gene            1356..2740
                     /locus_tag="VFV61_05455"
                     /pseudo
     CDS             1356..2740
                     /locus_tag="VFV61_05455"
                     /inference="COORDINATES: protein motif:HMM:NF009709.0"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
BASE COUNT          654 a          712 c          749 g          628 t
ORIGIN      
        1 aaaccggatt gttgctcgtg gtcacggatt ccgatgcttg caacaacggc aagcgggtcg
       61 ctctagccgc agccagttgc gcatcggcaa actggcggcc cgccgccgta cctcttgtca
      121 acgggttctt agcaagcgcg atttcgacag cttcagaaag tgtcagcggt tcgccagatt
      181 cggttacgac ccttggcgct gcgcccggct caatttgtga ctgccccggc actggatgag
      241 ctccgagatt taccagcccc agccccaggc tgaccacaag tgctttcttc atccaactat
      301 gcgacagcat ctaagctcac gcttccgatc tgcttgagaa ctgcccggcc tcgctctttg
      361 ctccgagcca acgaagaaaa ctgatcatcg ggcaccagtt tgtaaacgca gactggatca
      421 agttgatcgc tacaaagccg gtaaacaaaa accagtaagg actgtggaag taaccgagca
      481 gcagcgtggc tagcacgaag caaccggcga ttaatcgcaa atatcgttcg atggtcatga
      541 gatctctcct ttccagacaa aacgggagag agcaactgtc attccgagag agagctgatt
      601 aagcttgcct gtttttgagc atgggcgaag ttcaacagtt gcgaaattac gcaactgttg
      661 gccccaggct gcgaggtatt gcgcatcgga ttgtggttca gtaagggtac gtcaatctga
      721 atcaccttcg gttcaaagaa caaaaccaga gttgagtggg cactttgcaa tgcttctcgg
      781 gatcagcgca gttccccgtt aacgaagaag tttcggttaa cgcccatggc ccatggtttg
      841 ccacgcatgg gatgtgttgt ttagccgatc gcgattgagt gcgaggtttc ggcgttcgct
      901 cggctgttcc acgatctaag ccatgacagt caataaagta gttacaaaga tagaaaagga
      961 gatcttcgag cggcaggcgc gtatctgcaa agcgtttgct catcctgcac gattggagat
     1021 tcttgacctt ctgggccagg gtgaacgagg aggttccgag ttgcaggaag cgctaggcat
     1081 ttcgaagacg aggatgtctc aacatgtctc catcttgaaa tctgccggcg tgctgacgac
     1141 cagacgcgaa ggtaaacaac tctattgctc tctggcgatg cccgaggtga agcaagcatg
     1201 tcagttaata cgcaaagtgt tgcagggcca gattgcggca agcagccacc tggttccctg
     1261 aaggtttact tctgagggca atctgttagc gaaactgatc aatcatgaaa acgattattg
     1321 aaccctttag agtcaaggta gtcgaaccca taaaaatgac gacgcgcgcg gagcgcgctg
     1381 cctaccttga ggaagccgct tataacctgt tccggcttaa agcggaaaac gtgatcattg
     1441 acttgctcac cgacagcggt acggcggcga tgagtgccgg gcaatggtct gccctgatgc
     1501 gaggcgacga gtcctatgcc ggcagcaaga gcttctatcg ttttgaaaag gttgtgcgcg
     1561 acatctttgg tttcaggcag gtaattccca tgcaccaggg acgcgcggcc gagcgcatcc
     1621 tcttttccac tatgtgccgc aaaggaagca tcgtcccaaa taacacgcac ttcgacacaa
     1681 cacgcgccaa cgttgagtac ctcggagctg aagcagtcga tcttccaatt ccggaattct
     1741 atctaccggc cgtcactcat ccgttcaaag gcaacatgga aacggaggcg ctcgaacaat
     1801 taatcaagcg cgtaggcgcg gaacgcattc ctctcgtgat gctgacggtg acaaataatt
     1861 ccggcggcgg tcagccggtc tcaatggcta acatccgcga gcgaaaacga tctgcgcacg
     1921 ttacgggatt ccgctgtatt tggacgcctg tcggtttgcc gagaatgctt acttcatcaa
     1981 gctgcgcgaa gttggttacg ctgacaaatc ggtgctggag atcgcccgcg agatgttttc
     2041 gtatgccgac ggctgtacga tgtctgcaaa gaaagatggt ttgtcgaaca ttggtggctt
     2101 cctctgcaca aatgacgagc agttggccca acaggagaag gatctgctga ttctgactga
     2161 aggctttccc acttatggcg gtttggcggg gcgtgatctt gaggcgatcg cagttggtct
     2221 caaagaggtt ttgcacgagg actatttaca gtatcggttc gcttcaattc gttatttggg
     2281 cgagcacatc tcggatgcgg gcgtgcccat cgtgcaaccg cctggcggtc acgctatcta
     2341 catcgatgcg cgggcgctgc tgccccacat cccaccgctt caatatccgg gtcaggcatt
     2401 ggcagttgaa ctttaccgtg aggcaggcat tcgctcctgt gaaatcggct cggttatgca
     2461 cgcggagaaa gacgcggaga gcggtgcaga aatcccttcg cccatggatt tggtccggct
     2521 cgcaattccg catcgcgtct acacgcagag tcatatcgat tacgtgatcg aggcgatcct
     2581 gcgagtttat caacggcgcg actcgattca cggtttccgt atcattagcc aaccaaaatt
     2641 tctgcgccac ttcaccgcgt gcttcgagcc aattgacgaa ttagataacg tggagggagc
     2701 aaggagctat catggagaac aagtactttt acacaactga agt
//
LOCUS       DASUEX010000324         3104 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_281450, whole genome shotgun sequence.
ACCESSION   DASUEX010000324 DASUEX010000000
VERSION     DASUEX010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3104
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281450"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            113..1036
                     /locus_tag="VFV61_05460"
     CDS             113..1036
                     /locus_tag="VFV61_05460"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HEX4899958.1"
                     /translation="MPEFPDIVVYLEALDQRVLGETLKRITLGSPFLLRTAEPPLASV
                     QGQNVVALRRLGKRICFGFANDLWLVLHLMIAGRLHWKDDTRSTIKKGGAAKLPNKRN
                     LATFEFTNGALSLTEAGTQRRASLHLVAGEAALASLDPGGLEVFQSDLSQFASVLQSA
                     NHTLKRALTDPRLFSGIGNAYSDEILWHAKLSPIALTQKLQTEEIERLFNSTRALLTE
                     WVELLRAEAHGKFPEKVTAFRPEMAVHGKYQEPCPRCGSKVRRIRYAANETNYCPSCQ
                     TGGKLLADRALSRLLRADWPRSEEELERVLS"
     gene            complement(1137..1448)
                     /locus_tag="VFV61_05465"
     CDS             complement(1137..1448)
                     /locus_tag="VFV61_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899959.1"
                     /translation="MSRHEETFQIPLPPEQAKAVCGRAINSLNLPSSDLGYGFSCAES
                     FQFGFTWPVTMSVIIDHGDPGTSKITIGATNFGFGPIQKSHVRGKAITLRQRIEQMAT
                     G"
     gene            complement(1599..2558)
                     /locus_tag="VFV61_05470"
     CDS             complement(1599..2558)
                     /locus_tag="VFV61_05470"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEX4899960.1"
                     /translation="MNIVDVLLLLLILLSVFYGWRRGFILGVLDLLGWALVIISALRF
                     YQPLAKWLGANIDVWSESWDRPIAFIAIALTVGVLMQVAAHTLLRRIPKDVHERTANR
                     LFGLLPGVASGVITAALAAALLLAVPFSEALHERARESALVNRLAVYAQQLEAQLHPV
                     FAEAVAETLNLLTVQPDSHERVSLPYKITNARPRPELEARMLELVNKERLANGLRPLA
                     PDPELTVVARKHSGDMFSRGYFAHDTPEGRSPFDRMHDGGVSFRAAGENLALAPSLQV
                     AHSGLMNSPGHRANILGKDFGRVGIGILDGGMRGLMVTQNFRN"
     gene            2915..>3104
                     /locus_tag="VFV61_05475"
     CDS             2915..>3104
                     /locus_tag="VFV61_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899961.1"
                     /translation="MTHTRLLFCAVLLLFITATFGASSFAQRPGPQFWRRLSCEPLEP
                     RTKLEAFDERYGTVVVKGF"
BASE COUNT          731 a          838 c          844 g          691 t
ORIGIN      
        1 actcgaaacg taggcctcaa tgcaacttaa accgaactct tggtcgtgaa gatgaattct
       61 aaattggcaa ttggcaatcg gcaattggca atctcataag ttacgatgcc ccatgccgga
      121 atttcccgac attgttgtct accttgaagc tcttgatcag agggttctcg gtgagacact
      181 taagcgcatc acactcggtt cgccattttt gttgcgcacc gctgagccgc ctttggcgag
      241 cgtgcagggg caaaatgttg tagccctgcg ccgcctgggt aaacgcatct gctttggttt
      301 cgcaaacgat ctctggctcg tgcttcatct gatgattgcc ggacggctgc actggaagga
      361 cgacacgcgc agcacaatta agaaaggcgg ggccgcgaag ctgcctaata aacgcaacct
      421 ggccacattt gaattcacta acggagctct ttccctaacg gaggcaggca cgcagcggcg
      481 tgcttctctt catctggtag caggtgaggc agctcttgcc agtctcgatc ctgggggcct
      541 tgaagtcttt caatcggacc tctctcagtt cgcctccgtg ctgcagtcag ccaatcacac
      601 tttaaagcgc gcgctcaccg atccgcgttt gttcagtggc atcggcaatg cttattccga
      661 cgagattctt tggcatgcga agctttcgcc catcgcctta acgcagaaat tacagacgga
      721 agagatagag cgtttgttca actcgacgcg cgcgctgctg accgagtggg tggaactgtt
      781 acgcgccgaa gcccacggaa agtttccaga aaaggtgact gcctttcggc ccgagatggc
      841 cgtgcacgga aagtatcagg aaccgtgtcc tcgttgcggc agcaaggtgc gccgcattcg
      901 ttacgcagct aacgaaacga attattgtcc aagctgtcaa actggcggga agttgttggc
      961 agatcgcgcg ctgtcccgtc tgttgcgcgc cgactggccg cggtctgaag aggaattgga
     1021 gagggtcctc tcctaggtca gaagatgtag gggcgtccga cggaatgccg ccctaacagc
     1081 aagtctcgcg agtgtggcgt agctccaatc ggcaatcgcc aatcgccaat cgccaatcat
     1141 cctgtcgcca tctgttcaat acgctgccgg agtgtgatcg ccttcccgcg cacgtggctc
     1201 ttttggatcg gaccaaaacc aaagtttgtg gctccaatgg tgatcttact cgtgccggga
     1261 tcgccatgat caatgattac gctcattgtt acaggccagg tgaaaccaaa ctgaaaactc
     1321 tcggcgcacg agaaaccata gcccaaatct gagctcggca aattcaacga gttaatggcg
     1381 cgaccgcaaa cagccttggc ttgttctggt ggtaggggga tttgaaaggt ttcttcgtga
     1441 cgggacatga tcaattcatc aatcttgaaa ggaaactgaa gctagtcttt atttgttctt
     1501 tgtgcttggt actttgtact ttgtcttcgg aaatcaatca acttttaaaa acacagcacc
     1561 cgaatcaaag tacaaagctc aaagtacaaa gttcgttttt agttacgaaa attctgcgtg
     1621 accatgaggc cacgcatacc gccgtcgaga atgccaatgc cgacgcgacc gaagtcctta
     1681 cccaggatat tggcgcggtg acccggcgag ttcattaacc cgctgtgcgc cacctggagc
     1741 gagggtgcaa gtgccaggtt ttctccagca gctcgaaatg aaacaccccc atcgtgcata
     1801 cgatcgaacg gactgcggcc ttcgggcgtg tcatgcgcaa agtatccgcg gctgaacata
     1861 tctccggaat gcttacgcgc tacaactgtg agctctggat ccggggctaa gggccgcaaa
     1921 ccgttcgcga gtctctcctt gttaacgagt tcgagcatgc gcgcctccaa ttcgggccgc
     1981 gggcgtgcgt tcgtaatctt ataaggcaac gatacgcgtt catgcgaatc gggttggacg
     2041 gtaagcaggt tgagagtctc agctacggcc tcggcgaaga cgggatgcaa ctgggcctcg
     2101 agctgctggg catagactgc tagtcgattt acgagtgcac tctcgcgcgc gcgttcgtgc
     2161 aacgcttcgc tgaacggcac ggcaagcaac agggccgcgg ctaatgctgc cgtgatcaca
     2221 ccgctggcga ctccgggaag caaaccgaag aggcgattgg ctgtgcgctc gtggacgtct
     2281 tttggtatcc gacgaagcaa agtgtgcgca gctacctgca tcaacactcc gacagttaaa
     2341 gcgatggcga tgaaagcgat cggccggtcc cagctttccg accagacatc gatgttcgct
     2401 cctaaccatt ttgcgagcgg ctgataaaac cgcaacgcac taataatcac cagggcccaa
     2461 cccaacaggt cgaggacacc caggataaat ccgcgccgcc aaccgtagaa tacgctcagc
     2521 agaatcagga ggagcagcag gacgtcaacg atgttcactg agacagagta acaagtgatc
     2581 cgatgaaccg cgaatggcga cgcgaacgaa tcgagtgtct ggaatagcgg aacgttgacg
     2641 tgacggaggc gtgaactagg gatagagccg tgcgcctaac aaccaaaagc gcggagagtg
     2701 taggacgctg tcgcaggact gcgttgggga gtttttgagc aaagctggcc gaagctgcca
     2761 ggaactagtc agctaccctt attcttcgtt agcattcgca ccagactttt agtaagcgac
     2821 ctaatgcggg acgcattcac aaagctgtca tgacttttct tgacgctcgg gaacccatct
     2881 tgcaccgtaa cgaaacatat ccaaggagct ggttatgact cacacacgac tgcttttttg
     2941 tgctgttctg cttctattta ttaccgccac gttcggcgcc tcgagtttcg cgcaacgccc
     3001 aggtccacag ttctggcgcc gtcttagctg cgagcctctt gagccccgca cgaaactcga
     3061 agctttcgat gaacgctatg gaacggtcgt agtgaaaggc ttca
//
LOCUS       DASUEX010000325         2215 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_633246, whole genome shotgun sequence.
ACCESSION   DASUEX010000325 DASUEX010000000
VERSION     DASUEX010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_633246"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1010)
                     /locus_tag="VFV61_05480"
     CDS             complement(<1..1010)
                     /locus_tag="VFV61_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEX4899962.1"
                     /translation="MGKAIGIDLGTTNCCVAVLEGGAVQIVPNKEGGRTTPSVVGFTD
                     KGERLVGQIAKRQAVTNAANTVYAVKRLIGRKFNSEEVKRMRETSSFELVDSPNGDAR
                     IRVQGRSYSPPELSGIVLQRLKAAAEDFLGEAVTDAIITVPAYFDDTQRQATKDAGKI
                     AGLKVERIINEPTAAALAYGFGKSGVERIAVYDLGGGTFDISILEMNEGAFDVLSTSG
                     NTFLGGEDLDERIMSWMADQFLADTGIDLRKDRLALQRLKEVAERAKCELSSANEANI
                     NLPFIAADATGPKHFNKTLTRAEFEGLVGELIEATVEPCNKALADAKLKPENIDKVIL
                     VGG"
     gene            complement(1014..1760)
                     /locus_tag="VFV61_05485"
     CDS             complement(1014..1760)
                     /locus_tag="VFV61_05485"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEX4899963.1"
                     /translation="MPDKKTRSNRIPIRYPDSEEESEISSRSNNEDFAEAQDEQEDPL
                     DEGGSVLPDTDEEAEATLPFGDDAGLFPNVQRGSGELGGPLVAELVATRAELKRVEGE
                     MAELKDRLARRQADFENYRKRVERERSETYERVVAEIAAKLLPVLDNLKRALDTEASM
                     EAGESDEFRHFLSGVDLIFKQLSGVIEALGVKPVLAVGERFDPHIHEAVVSEATDEHE
                     PDTVMEEIIRGYRMGDKLIRPALVKVAIKK"
     gene            complement(1803..>2215)
                     /locus_tag="VFV61_05490"
     CDS             complement(1803..>2215)
                     /locus_tag="VFV61_05490"
                     /inference="COORDINATES: protein motif:HMM:NF013768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HrcA family transcriptional regulator"
                     /protein_id="HEX4899964.1"
                     /translation="LLCDQSLGGDASVGDVYVDGASNMLTKPDFVDVERMRDLFKTFE
                     EKSRLVKILNECLSREQSFHGDVNVVIGREHPTSSMRNCALITAPYRIGMNENVGTLG
                     VVGPMRIEYSRIMAMVNYMARLIERRLSEENSLY"
BASE COUNT          469 a          664 c          542 g          540 t
ORIGIN      
        1 ccaccaacca ggatcacctt gtcaatattc tccggcttga gtttggcgtc tgcaagagcc
       61 ttgttgcagg gttcgacggt ggcctcgatt aactccccga ctaaaccctc aaactccgcg
      121 cgcgttagag tcttattgaa gtgctttggt cccgtggcat ccgcagcgat gaatggcagg
      181 ttgatatttg cttcgtttgc cgatgacagt tcacatttcg cgcgctcggc aacttccttg
      241 agacgctgca gggcgagacg atctttgcgc aggtcgattc cggtatcagc aaggaactga
      301 tcggccatcc agctcatgat ccgttcatcg agatcttccc cacccagaaa agtgtttccg
      361 gaagtagaga gcacatcaaa cgcgccttca ttcatttcca gaatcgaaat atcgaaggtg
      421 ccgccgccaa gatcgtagac cgcaatccgt tccacaccag actttccaaa tccgtaggct
      481 agcgccgcgg ccgtcggctc gttaatgatt ctctcgactt tcaacccggc aattttgccg
      541 gcatccttag tcgcctgacg ttgcgtgtca tcaaaatagg cggggactgt gataatcgca
      601 tccgtcacgg cctccccaag aaaatcctcg gcagcggcct tcaggcgctg aagcacgatc
      661 ccggaaagct ccggcgggct ataactacga ccttgaaccc ttatccgcgc atcaccatta
      721 ggcgaatcaa ccagttcaaa ggatgaggtc tcgcgcatgc gcttgacctc ctcagaattg
      781 aactttctgc cgatgagacg tttcaccgca tagacggtat tggccgcgtt ggtgaccgcc
      841 tggcgcttgg caatctgccc caccaggcgc tcgcccttat cagtaaagcc aacgacgctg
      901 ggcgtggtgc ggcccccttc tttgttcggc acaatctgaa cagcaccgcc ttccaacacg
      961 gcgacgcagc aattagtcgt gcccaaatct atgccgatag cttttcccat cttctatttt
     1021 tttatcgcta cttttacgag ggctggtcta atcagcttgt cgcccatcct gtagccgcga
     1081 atgatttctt ccatcacagt atcgggttcg tgttcgtcgg ttgcttccga gacgactgct
     1141 tcgtgtatat gaggatcaaa gcgttccccg accgccagaa ccggtttgac tccgagcgct
     1201 tcgatgaccc cactgagttg tttgaagatc aaatcgacgc cgctgaggaa gtgacgaaac
     1261 tcatcagatt cgcccgcttc catcgatgct tccgtatcca gggcgcgctt cagattgtcg
     1321 agtaccggca acaattttgc ggcaatttcg gccacgacgc gttcgtaagt ctcgctgcgt
     1381 tcacgctcca ctcttttgcg gtagttctca aaatccgcct gcctgcgcgc cagccggtcc
     1441 ttcaactcgg ccatttcgcc ttcgactcgc tttagctcgg cgcgcgtcgc taccagctcg
     1501 gcaaccaaag ggccgcccaa ttcgccgctg cctctctgaa cattgggaaa caagcccgca
     1561 tcgtccccaa aaggtaacgt tgcttcggct tcctcgtccg tgtcaggcag aactgaaccg
     1621 ccttcgtcta gcggatcttc ctgttcgtcc tgagcttcgg cgaagtcttc gttattggat
     1681 ctgctggaaa tttcgctctc ctcttcggaa tccgggtacc tgatgggaat gcgattcgat
     1741 ctggttttct tatcgggcat taatcaagaa caccgcttgc gtagacgagc ccttgggcag
     1801 gctcaataaa gtgagttctc ttcgcttagg cggcgctcaa taagccgcgc catgtagttt
     1861 accatagcca tgatgcgtga atattcaatt ctcatgggac ccaccacgcc gagcgtaccg
     1921 acattctcgt tcatgccgat ccgataggga gccgtgatca gcgcgcagtt tcgcatcgag
     1981 ctggtcggat gctcgcgacc aattacaacg ttgacgtcac catggaacga ctgctcgcga
     2041 gaaaggcatt cgtttagaat cttgacgagg cgcgatttct cctcgaaggt tttgaacaaa
     2101 tctcgcatgc gttcaacgtc gacaaaatcc ggcttggtca acatgttcga cgcgccatcg
     2161 acgtaaacat caccgacgct ggcatcgccc ccaagactct gatcgcaaag caaga
//
LOCUS       DASUEX010000326         2606 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1098703, whole genome shotgun sequence.
ACCESSION   DASUEX010000326 DASUEX010000000
VERSION     DASUEX010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1098703"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1689)
                     /locus_tag="VFV61_05495"
     CDS             complement(<1..1689)
                     /locus_tag="VFV61_05495"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX4899965.1"
                     /translation="MKRLGIVVQRCHESVVGGAEALAWQYANLLKDDYEVDVLTTTAV
                     DAAYWANALAAGVELRDGITIRRFHVDIGYTPYRSMLFGYLLQDFNKYQPSVKNRNGG
                     GAAAAGTHLPWSISLQEELIRHIGPYSRSLIKFIKENHAKYRTLIFVTYLYPTSYFGL
                     LQVPPGFALFAPTLHDEQPAYLSAYKHAARRAGSLIWLSEGEQRLGVGLWGELPGSVV
                     GMHIDATPRPPKPGSTPYLLYCGRIDPNKGCGELFDYFLQFKQEHPSNLRLVLTGKDD
                     TAIPDHPDIDFRGFVSEEEKFALMAGAAAYMMPSGKESFSIVTLEAMAQRAPVLASGA
                     SEVIVDHIRRSKGGRIYNDYHGFASALDELLGNADLRRDMGERGREYVLANFTAEIVK
                     RDLAAAVEACVAAFNSRSAPVTSQAPSVPEISNYVEPKSLDLSYTAPQALPAGWTEVA
                     LREFVTSIQVEDGPSAELKNYANDDFKRFIYTLGLVPEEPGLRILELGSNPYFTTTLL
                     IKFRQPELHLANFFGAGKGSEGNQNVAIHQTGEVVNYSYKHFNVEEDEFPYADNM"
     gene            complement(1686..>2606)
                     /locus_tag="VFV61_05500"
     CDS             complement(1686..>2606)
                     /locus_tag="VFV61_05500"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX4899966.1"
                     /translation="AGFLKKPDDVLIYHYSRGWDLGLKLLQNRRSHTVVRYHNVTPPE
                     FFVGYNADLAAMCHAGRLQLHPIANSQSDRYLSASAFNMRELLSAGANESSSFVVPPF
                     HHIDRLLSVPAARKVLEAYGDEKTNICMVGRVAPNKGHAALIEAFAAYHHDYNKESRL
                     LIIGKEETRLRNYSPFLRELANRLEIRDAVVFAGEVSDEALRAYYQSAHAFMITSEHE
                     GFCVPLVEAMALGVPIVAFASSAIAETVADAGIVWEERNPYLLAETLDRVLKKSISVD
                     FSLKGRRRYEEHFTNEKIEAAFFNALSGIL"
BASE COUNT          596 a          724 c          694 g          592 t
ORIGIN      
        1 catgttgtca gcatagggaa actcatcctc ttcgacattg aagtgtttgt agctgtagtt
       61 aacgacctcg ccggtctggt gaatcgcgac gttctggttg ccctcggacc ctttacccgc
      121 gccaaagaag tttgccaggt gcaattcggg ctgccggaac tttattagca acgtcgtggt
      181 gaagtaagga ttggatccga gttccagaat ccgcagaccc ggctcctcgg ggaccagacc
      241 taaggtgtaa atgaaccgtt tgaagtcgtc gttggcgtag tttttgagct cagctgaagg
      301 gccatcttcc acctggatcg acgtcacgaa ttcgcgtaat gcaacttcag tccaaccggc
      361 gggcaacgct tggggcgccg tgtaagaaag atcaagagat ttgggttcaa cgtagttcga
      421 gatctcaggg accgagggcg cctgcgacgt tacgggcgca gaccttgaat tgaacgccgc
      481 aacgcacgct tccactgcgg cagccaaatc tcgtttcact atctccgccg taaaatttgc
      541 cagcacatac tcacggccgc gctcgcccat gtcccggcgc aagtcggcat ttcccagcag
      601 ctcgtcgaga gccgaggcaa acccgtggta gtcgttataa atccgcccgc ctttgctgcg
      661 gcgaatgtga tcaacgataa cttcagaggc accgctcgca agtaccggcg ctctctgcgc
      721 cattgcttcc agagtgacta tgctgaaact ctctttaccg gacggcatca tgtaagcagc
      781 cgcacccgcc atcaacgcga acttctcttc ctcggaaacg aacccgcgga agtcaatatc
      841 cggatgatca gggatcgctg tgtcgtcctt ccctgtaaga acgagcctca ggtttgaagg
      901 atgctcctgt ttgaattgca ggaagtagtc aaagagctcg ccgcaacctt tattcggatc
      961 tatgcgcccg caatacaaca gatacggagt tgagcctggc ttgggcgggc gaggcgtcgc
     1021 atcgatgtgc atgcccacca ctgaccccgg tagttcaccc cagagaccaa cacccagtcg
     1081 ctgctcacct tcgcttaacc agatcagcga accggctcgc cgcgccgcgt gcttgtaagc
     1141 tgagagatac gcaggttgct cgtcatgaag cgttggcgcg aagagcgcaa atcccggtgg
     1201 cacctgcagc aaaccaaaat aagaggtggg ataaagatag gtgacaaaaa ttaacgtgcg
     1261 gtatttcgca tggttctctt tgatgaactt tatgagcgac cttgagtaag gcccgatgtg
     1321 acgaatgagt tcctcctgca gcgagatgct ccacgggaga tgagtccccg cagctgccgc
     1381 gccaccacca tttctatttt taacgctggg ctgatactta ttgaagtctt gcagcagata
     1441 gccaaataac atactgcggt aaggtgtgta gccgatatca acatggaaac gcctgatcgt
     1501 tatgccgtca cgcagctcca cgcctgcggc caaggcgtta gcccagtagg cagcgtcaac
     1561 tgcggttgta gttagaacgt caacttcgta atcgtctttc agcaagttgg catactgcca
     1621 ggcgagagct tccgctccgc caaccacgct ctcatgacag cgctgcacaa ctatgccgag
     1681 tcgcttcata gaattccgct caaggcattg aaaaatgctg cttcgatctt ctcgttagta
     1741 aagtgttctt cgtaacgccg ccgccctttt aagctgaaat caacgcttat cgatttcttg
     1801 agcacgcgat cgagtgtctc agcgagcagg tacgggtttc gttcctccca gactatgcct
     1861 gcgtctgcaa ctgtctcggc aatcgccgag gaggcaaagg caacgatcgg tactccgagc
     1921 gccatggctt cgacgagtgg tacacagaag ccttcgtgtt cgctggtaat cataaaggca
     1981 tgggcactct gatagtacgc tctcagcgct tcatccgaga cctcgccggc gaatacgact
     2041 gcgtctctaa tctcgaggcg attggccagc tcgcgcagga aaggtgaata gtttcgcagg
     2101 cgagtctctt ccttgccaat aataagtaag cgactctcct tgttgtagtc gtgatggtag
     2161 gccgcaaagg cctcgattag cgctgcatgc cccttattcg gcgccacacg tccgaccatg
     2221 cagatatttg tcttttcgtc tccatacgct tccagcactt ttctcgccgc cggaaccgaa
     2281 agcaggcgat cgatatgatg gaacggcgga acgacgaagc tgctcgattc gttcgctcct
     2341 gcggacagca attcccgcat gttgaaagcg gatgcagata agtagcgatc actctgggaa
     2401 tttgcgatgg gatgcaactg cagacgtccc gcgtggcaca tcgcggccag atcagcattg
     2461 tagccaacga agaactcagg gggtgtgacg ttatggtatc tgactaccgt gtggcttcgg
     2521 cgattctgca agagcttcag acctaagtcc cagccccggg agtagtgata aatcagaaca
     2581 tcgtcgggtt tcttcaggaa gccggc
//
LOCUS       DASUEX010000327         3586 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_67256, whole genome shotgun sequence.
ACCESSION   DASUEX010000327 DASUEX010000000
VERSION     DASUEX010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3586
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67256"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="VFV61_05505"
     CDS             complement(<1..261)
                     /locus_tag="VFV61_05505"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX4899967.1"
                     /translation="MPSNPSSLDPSNGVDSERNGFAEVRRDLLTSDSASVRAAAARQL
                     GSLRNQAGIAILIVALQDNSPEVRSAAVEALRLIGDATALGPL"
     gene            682..1530
                     /locus_tag="VFV61_05510"
     CDS             682..1530
                     /locus_tag="VFV61_05510"
                     /inference="COORDINATES: protein motif:HMM:NF016143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEX4899968.1"
                     /translation="MRWRGQRESENIEDRRGMSRGGIAIGGGLGSIVILIIALLLGAD
                     PRQLLEQAPVDPGTATQTSRSTNPEEEELRRFTSVVLASTEDVWTDIFRQAGANYRKP
                     TLVLFTDQVSSACGQAGAAVGPFYCPGDEKLYLDFAFFHDLQTRFRAPGDFAQAYVIA
                     HEVGHHVQRLLGTMDRVNAAQRQRGEAEANALSVRLELQADFLAGVWAHYAQQKGLLE
                     QGDIEEALGAASAVGDDRLQRESQGYVVPDSFTHGSSEQRIRWFRHGLETGDIRQGDT
                     FNTRNP"
     gene            complement(1533..2288)
                     /locus_tag="VFV61_05515"
     CDS             complement(1533..2288)
                     /locus_tag="VFV61_05515"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX4899969.1"
                     /translation="MNLKLLSYNIRFGGRGREARLAEVIRTVSPDLVVFQEATDPLVI
                     ERLASATDSPYWSAQRSHSIAYISRIEIAHHEWHYPAGAKHSFLEIVPDGSETRIFGL
                     HLSARFSKWDERQRAREIRNLLQGIERHQSGFHVLVGDFNTLAPGEVLQVQKMPAWIR
                     ALVWISGRDIQRETIQVMLDAGYADGYRRLHPDIKGYTFPTWDPHLRLDYVFVPTAFA
                     NRLKTCEVVTEAPALTEASDHLPLLAHLDLNGG"
     gene            complement(2288..2743)
                     /locus_tag="VFV61_05520"
     CDS             complement(2288..2743)
                     /locus_tag="VFV61_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899970.1"
                     /translation="MSEEEIRERVLLLGFGGDQRLYMSFCKRLQADLPPGTGVALRGS
                     VVTNKKWEDGRPFDADGKGTSDLDVTLVGTKVMEYWDKDAFYIPGLHTKPLCDDDPTI
                     AIGLNKLRKDLQNLVGRPVNMQATSNLVLYARDVLFGEPYFTAIEPEKE"
     gene            2995..3459
                     /locus_tag="VFV61_05525"
     CDS             2995..3459
                     /locus_tag="VFV61_05525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899971.1"
                     /translation="MISAKVKIRLTILLGLVAIAAVNAPVVRAQTSSALPNPVLVFLG
                     SEYYQANGKSFVRYRFEVHNHGAYPDSLFAAAPALPPCGKNTNASRTWLDFYDMQGRR
                     LYGFCALGSHKDLVGIWFALEEGVVPPSWVYIEMTDRQTGTKYKSNLADTTI"
BASE COUNT          803 a          977 c          990 g          816 t
ORIGIN      
        1 aagtgggccc agcgccgtcg cgtcaccgat cagtctcaac gcctctactg ccgcgctccg
       61 cacctccggc gagttgtcct ggagcgcgac gatgagaatg gcgatgccgg cttggtttct
      121 cagtgaacca agctggcgag cggctgcggc gcgcacgctg gccgagtcag acgtgagcag
      181 atcgcgcctc acttctgcga agccgttacg ctcggagtcc actccgttgg aaggatcaag
      241 cgaggaagga ttgctgggca tgcttgggta atcgacctcc gggcattgtg agcgagttac
      301 aacaagggag ttcaaagccg ctcggggttg agacgcatga gtaaacgcat gacacggtag
      361 caccgcgtcg gcggattttc gtctcttggg attgttcgaa gagtagcgtc ggtgagtcaa
      421 gctcttcgaa tactggcaat ggccttagtg ggctaactag tacggccaaa acgattctct
      481 gctatgtaaa ggctgtcgtc aatacccttt tgcgcggtac ttagccgttt tccgaggctt
      541 tggccatttt tcccgcgatc gcggtgagtt tttccggagt ccagccagcc aggcttccag
      601 aagttttctg ccaaaacgag ttcgcgtgcg ataattccca gactcattaa cactaagacg
      661 tcttaccggg aggggcgagg tatgcgctgg agagggcagc gggaaagcga gaacattgaa
      721 gaccgccgcg gaatgtcgcg cggcggaatt gcgatcggcg gaggtctcgg cagcatcgtg
      781 attttgataa ttgcgttgct gttgggcgca gatccacgtc aactactcga acaagcccct
      841 gttgatcccg gcacagcaac gcaaacgtcg cgttcgacga atcccgagga agaagagctc
      901 aggcgtttta cttcggtcgt tctcgcgagc accgaagacg tttggaccga catcttccgc
      961 caagcgggcg caaactatcg taaacctacg ctcgtgcttt tcactgatca ggttagttcg
     1021 gcgtgcgggc aagctggagc tgcagtcgga cccttctact gtcccggtga tgaaaagctc
     1081 tatctcgatt ttgcgttctt ccatgatctg caaacacgct ttcgcgctcc cggagacttt
     1141 gcccaagctt atgtgatcgc gcatgaagtt ggtcaccacg tgcagcgatt gttgggaacc
     1201 atggatcgcg ttaacgcagc ccaacgccag cggggcgaag ctgaggcgaa tgccctttca
     1261 gtgagattgg aactgcaagc agattttctg gcgggagtct gggcgcacta cgcgcagcag
     1321 aagggcttgc tcgagcaagg cgacattgag gaagctctcg gagcagccag cgcagttggg
     1381 gacgatcgac tgcaacgcga atcgcaaggt tatgtcgtgc cggattcgtt tactcatgga
     1441 agttccgagc aacgcattcg ttggtttcgc cacggacttg aaactggaga catccgccag
     1501 ggagacactt tcaatacgcg caatccgtaa aatcaacctc cattcagatc caggtgtgcc
     1561 agcagcggca aatgatccga agcctctgtc aacgcgggcg cttccgtaac tacctcacag
     1621 gtttttaagc gattggcgaa agccgtcggc acaaagacat aatccagccg caggtgtgga
     1681 tcccacgtag gaaaggtgta gccctttatg tccggatgga ggcgccggta accgtcagca
     1741 tatccagcgt cgagcatgac ctggatcgtt tcgcgctgga tgtcgcgtcc actgatccaa
     1801 accagcgccc ggatccaagc cggcatcttt tgcacttgca gcacctctcc cggagcaagg
     1861 gtgttgaagt cgcccaccaa tacatgaaaa ccgctttgat gtcgttcgat accctgaagc
     1921 aggttgcgaa tctcgcgcgc tctctggcgc tcatcccact ttgaaaagcg agcactcagg
     1981 tgcaaaccaa aaattcgagt ctcgctgccg tcggggacga tttccaggaa tgaatgtttg
     2041 gctcctgccg ggtagtgcca ttcgtggtgg gcaatctcta ttcgactgat ataggcgatg
     2101 gagtgagatc gttgtgcgga ccagtaagga gaatcggttg cgctcgcgag ccgttcaatt
     2161 accagcggat cggttgcttc ctggaaaaca accaggtcgg gcgagacggt ccgaataacc
     2221 tccgccaagc gcgcctcgcg tccccgtcct ccaaaccgga tgttgtagct gaggagtttg
     2281 aggttcacta ctctttttca ggttcgattg ccgtgaagta aggctcacca aagaggacgt
     2341 cacgcgcata gagcacgaga ttggaagtgg cttgcatgtt aaccggtcgg cccaccaggt
     2401 tctgtagatc ctttcgcagt ttgttaagac caatggcgat agttggatcg tcatcacata
     2461 agggtttcgt gtgtaatccc ggaatataga aggcgtcctt gtcccaatat tccatcacct
     2521 tagttcctac gagggtgaca tcaagatcac tcgtaccttt accgtcagca tcgaagggtc
     2581 tgccatcttc ccacttcttg tttgtcacca cactgccacg cagcgcgacg cctgtacccg
     2641 gtggcaagtc agcttgcagt cgcttgcaaa acgacatgta gagccgctga tccccgccga
     2701 aacccaatag caaaacacgc tcgcgaattt cctcttcgga catttcctta acttccttgg
     2761 ccatgttggt tcccccctgc ccagtgtgcc tgcatgatac ttcatgctcg caaaagcggc
     2821 atctcacggg gttaccaacg cttcgcccgg ttgagtaata ttggttgaaa tttgtcatca
     2881 tgagcgctag gctgtcggcg ctcgacctcg taacttcact ccagcaatcc aactttgatc
     2941 gcggttaagg agactcctcc gcaggttctg actgtaaccc gaaaggaagg gatgatgatc
     3001 tcagccaaag taaaaatacg tctcacaatt ttgctgggcc tggtggcgat cgcagcggtg
     3061 aatgcccccg ttgtgcgcgc tcaaaccagt tcagcgttac caaacccggt gttggtgttt
     3121 ctgggctccg agtactacca ggccaacggc aagtcgttcg ttagatatcg attcgaggtc
     3181 cataatcatg gggcgtatcc cgattcccta tttgccgccg ccccggcgtt gccgccgtgc
     3241 ggaaaaaaca ccaacgcctc gcgcacctgg cttgacttct atgacatgca gggccgacgc
     3301 ctttacggat tttgtgcttt aggaagtcat aaggatctgg tgggcatctg gttcgcgctg
     3361 gaggaaggcg ttgttccgcc aagttgggtc tacattgaga tgacggatcg gcaaaccggc
     3421 accaaataca aatccaattt agccgacact acgatttaag agttttgagg cactcgggtt
     3481 cgaggtttac tgtgctcgga gagcaaactc gcggcgcaaa acccaatcca aatttgacag
     3541 ctcgaactac aaaccgaaac actacagatc attcaggaga accgat
//
LOCUS       DASUEX010000328         2219 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1305563, whole genome shotgun sequence.
ACCESSION   DASUEX010000328 DASUEX010000000
VERSION     DASUEX010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2219
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1305563"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1468)
                     /locus_tag="VFV61_05530"
     CDS             complement(<1..1468)
                     /locus_tag="VFV61_05530"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX4899972.1"
                     /translation="MLTLVPLTQIRRFGLLLLVSATILVALPQAASFAQTGTPAPLID
                     RELFFGDPEISGAQISPDGKFIAFVKPFKGTRNIWVKRTEEPFTAAKPITADKSRPVT
                     QYFWSRDGRYILFAQDKAGDENYNVYAVNPSDNPAAGEDVPPARNLTDAKGIRAALYA
                     VPRSEPDAIYIGLNDRDKAWHDLYKVKISTGERTLIRQNTERITGWIFDLKDQLRLAS
                     RSAENGDTEVLRVDDKGFTKVYSCNVFESCGAIQYHKDGQRVYFISNKGAANDLIKLE
                     LFNPVTGKEELVESDPLNRVDFGGASFSEVTDELIWTTYTDEKPRIYWKDKSFEADHK
                     LLQKKLPGKEVVFNGSTKDERLWLLTAYSDTEPGERYLFDRQTKKLTLQYRTRERLSR
                     EALAPMKPVRFKSSDGLEIPGYLTLPKGVAAKNLPAMIVPHGGPWARDGWGYNSFAQF
                     LANRGYAVLQPNFRGSTGYGKKFLDAGNKEWGQKMQDDV"
     gene            complement(1635..>2219)
                     /locus_tag="VFV61_05535"
     CDS             complement(1635..>2219)
                     /locus_tag="VFV61_05535"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX4899973.1"
                     /translation="KHGGLFIADEVQTGWGRTGEKWFGIEHWDVEPDIITSAKGLGNG
                     VPIGITVATPEVADSYPGLTFSTFGGNPVSMAAALAVIRMIEEDDLKKNAAQVGAYLR
                     QNLEMLKEKHPVIGDVRGMGLMQGLELVKDRETKEPAPQALLRVFEETKRRGVLIGKG
                     GLYGNVIRTGLMLNSTRDHVDQLVSALDAGFAAV"
BASE COUNT          482 a          617 c          574 g          546 t
ORIGIN      
        1 taacatcgtc ctgcatcttc tggccccact ctttgttacc cgcatcgaga aacttcttgc
       61 cgtagccagt ggaaccgcgg aagtttggtt gcaagacagc gtaaccgcga ttggcaagaa
      121 actgtgcgaa gctgttgtag ccccacccgt cacgggccca gggaccaccg tgcggtacga
      181 tcatcgcggg caggttcttg gcagccacac cttttggcaa agtgagatag cccggtatct
      241 ccagaccatc ggacgacttg aagcgaaccg gcttcatcgg agcgagcgct tcgcgcgaca
      301 gtctttcccg cgttcgatat tgcaaggtta gtttctttgt ctgccgatca aagagatatc
      361 gctcacccgg ttccgtatcg ctgtaggctg tgagcaacca gagacgctca tccttagtgg
      421 aaccattgaa tacaacttct ttgccaggca gtttcttttg cagcagcttg tgatctgctt
      481 cgaatgattt gtccttccag taaatgcgcg gcttttcgtc ggtataggtg gtccaaatca
      541 actcatcggt cacttcagaa aacgacgctc caccaaagtc gacacggttc aggggatcgg
      601 attcaaccag ctcttccttg ccggtgaccg gattgaacag ctcgagtttg atcagatcat
      661 ttgctgcgcc cttattcgat atgaagtaaa cgcgctgacc atccttgtga tactgaatag
      721 caccgcagct ttcgaagaca ttgcaggagt acactttggt gaaaccctta tcgtccacgc
      781 gcaagacctc agtgtctcca ttctccgcag agcgtgaagc aagacgaagt tggtctttga
      841 gatcgaagat ccagccggtg atccgctcag tgttctgccg gatcagagtg cgctcgccag
      901 tcgaaatctt gaccttgtaa agatcgtgcc aggctttgtc gcgatcgttg aggccgatgt
      961 agatcgcgtc tggttcacta cgtggtacgg cgtagagtgc cgcgcggatt cctttggcgt
     1021 cggttaagtt gcgcgccggc ggcacatctt ctccggccgc cggattgtcg gacggattaa
     1081 cagcgtacac gttgtaattt tcgtcacccg ctttgtcctg tgcgaagaga atgtacctgc
     1141 cgtcgcggct ccagaagtac tgggtcaccg gtcggctctt gtcagccgtg atgggcttgg
     1201 cggcagtgaa gggttcttcc gttcgcttca cccaaatatt tcgggtgcct ttaaatggct
     1261 tcacgaacgc gatgaatttt ccatcgggtg aaatttgggc gcctgaaatt tccggatccc
     1321 cgaagaacag ttcgcgatcg atgaggggag cgggagtacc agtttgcgcg aaactcgctg
     1381 cctgcggtag cgccacgaga attgtggcag aaaccaacag caataaacca aacctacgaa
     1441 tctgtgtaag tggaactaaa gtcagcataa cgcctcttct ttcttgtggg actgaaaggg
     1501 tttgaatcca gaatagcgta cgtgtttacg gctctgactg ccgctgagtt tcatcagaat
     1561 ggctcaccca atgagcaggt aaagctttca cgatttcgta cagtttgtgg atcaggcccc
     1621 ggtacagcgg acatctacac cgcagcaaat ccggcgtcaa gagcactgac gagttgatcg
     1681 acatgatccc ttgttgaatt gagcatcaag ccggtgcgaa tcacattgcc gtaaaggccg
     1741 ccttttccaa tcagcacacc gcgacgtttc gtctcctcaa agacccgcag caacgcctgc
     1801 ggagccggct ccttcgtttc gcgatccttg actaattcaa ggccctgcat caggcccata
     1861 cctcgcacat cccctatgac agggtgcttc tccttaagca tctccaggtt ttgccgcaga
     1921 taggcaccca cctgggcggc gtttttcttt agatcgtctt cctcaatcat acgaatcacc
     1981 gccagtgcgg ccgccattga gacggggttg ccaccgaaag tggagaacgt tagccccgga
     2041 tagctgtcag caacttccgg tgtggcgacc gtaattccga tcggtactcc gttacccaat
     2101 ccctttgcgg aagtgatgat gtctggctca acatcccagt gttcaattcc aaaccatttt
     2161 tcgcccgtgc gtccccagcc tgtctgtacc tcgtcggcga taaagaggcc gccatgctt
//
LOCUS       DASUEX010000329         4337 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1436794, whole genome shotgun sequence.
ACCESSION   DASUEX010000329 DASUEX010000000
VERSION     DASUEX010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4337
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1436794"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="VFV61_05540"
     CDS             complement(<1..446)
                     /locus_tag="VFV61_05540"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEX4899974.1"
                     /translation="MKILAIAPHPDDETLGCGATLLKHKTSGDKLYWLIVTRGHEPEW
                     SREVITAKEAEIEKVSTAYGFQKTFRLGFPTVRLDSVPLEQLISAIREVIEEIKPDYV
                     YLNHFGDVHSDHRVVFEATMSVLKPFYSSRHGVRKVLSYEILSSTDA"
     gene            complement(454..1164)
                     /locus_tag="VFV61_05545"
     CDS             complement(454..1164)
                     /locus_tag="VFV61_05545"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX4899975.1"
                     /translation="MEATVKPKTEAHKRWNKVYSEGFMTMWYPHEDIIRFCARLIQRK
                     LTHDRYEVKRKVERVLDLGCGNGRNAMYFARQGFSASGLDVSTQAIEWAKDWSKREGL
                     EIDFRVGDIEQLPFENETFDVVVSHGVLDHVPMPTARNAATEVHRVLKPGGLFYCDLR
                     STEDFEFGEGEEVAPNTFVVSEGFEQGLVQHFFSEDETKALFDGLFGIIYSEIAESRL
                     GPDFKRKYSRWVFATEKL"
     gene            complement(1177..2334)
                     /gene="neuC"
                     /locus_tag="VFV61_05550"
     CDS             complement(1177..2334)
                     /gene="neuC"
                     /locus_tag="VFV61_05550"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: protein motif:HMM:TIGR03568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HEX4899976.1"
                     /translation="MAKRRVLGITAIRSEYFLQRSIFRAIMDHPELELSLIVTGAHLS
                     PLHDYSVREVGRDGMPITERVESLIHSDHDAARLKGAAAQLQVLAHIVDRARPDWLLV
                     PADREEAMTLALCGAYLNVATAHYAAGDRVVGNVDDMVRHSVSRLAHILLTTHEAARE
                     RLIRSGEEEWRVHNVGHAGLDRFKTAPELRPTELASALGIEKIEQPYLVVIQHPLSSE
                     TNMSAQQMRETMEAVVDLRMQTFVSYPNSDPGSLEMIRTIEDYRQNPLINIFHNIPDD
                     IFVNLLRGAAALVGNSSLGLLEAPFLHLPVINAGRRQSGRKHSENVFFVSNDRGEIAA
                     QVRKLQTDEATRRLVENCSNPFGDGRTGTRVAEILASTPIDQRLLNKDLAY"
     gene            complement(2334..3041)
                     /locus_tag="VFV61_05555"
     CDS             complement(2334..3041)
                     /locus_tag="VFV61_05555"
                     /inference="COORDINATES: protein motif:HMM:NF020463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HEX4899977.1"
                     /translation="MKVAIHQPQYFPWPPYVHKVMSADIFVYLDTVQFSKNGLQNRNQ
                     IRAINGPAWLTVPVTHKFGSSIRETAIANGRTLLKHWKTLQANYSRSEGFQRWRDELA
                     ALFQEESASLADLAINSTEWMLKKLDVQNKRILASELPGIEGQASELVASICKHLGAT
                     RYLTGTGALAYLNPADFSHIGCELWVQQWRPITYHQAPRQTAFVPDLSTLDLLLNCPD
                     QAGEIIKAAGSWRRLEQ"
     gene            complement(3038..4216)
                     /locus_tag="VFV61_05560"
     CDS             complement(3038..4216)
                     /locus_tag="VFV61_05560"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEX4899978.1"
                     /translation="MPTNYKPDPAHPLTTAYPYFPPAEQEWLNQELAQILNGQLAMGP
                     RVRQFEREFAAYCRLPYGVAFPSCTSSMEAALVALGVGPGDEVLVPVETFVATGMVVS
                     LVGARPVFTEISAKTFSMDFDDAWSRVTERTRGAIVVHFGGFISPELPEFVRRMRETG
                     RFVIEDAAHAPGAELGGKRAGSLADAGCFSFYPTKIMTTGEGGMLVTAREDLARTVRS
                     LQNRGRDMEERAELYRLPGRNNRFTEISAAMGLSQLRCLPEFLESRRRVAAIYDELLL
                     GSELFVPLLAEAGSNPSYWRYVAMPTVTIDRTVLAAQLAQDGITIDWAYDPPLHLQPV
                     FQNMLGTQPGMLPMSEELLSRHICLPVHARMREEDAAYVVERLLEHVSSLSRTSRQSA
                     "
BASE COUNT         1004 a         1215 c         1118 g         1000 t
ORIGIN      
        1 gcatcggtgg aagacagaat ctcgtaactc aacaccttgc gaacgccatg gcgcgagcta
       61 tagaagggtt ttagaactga cattgtagct tcaaagacga cgcggtggtc ggaatgcacg
      121 tcaccaaagt ggttcaaata cacatagtca ggctttatct cttcgatcac ttctctgatt
      181 gccgaaatca actgttcgag ggggaccgaa tccaatctca ctgtgggaaa gccgaggcgg
      241 aaagtctttt gaaatccata ggccgtgctg accttctcaa tctcagcttc ctttgctgtt
      301 atcacttctc gcgaccactc aggctcgtga ccgcgagtga cgatcaacca atagagcttg
      361 tcgccgctcg tcttatgttt caagagcgtg gcgccgcaac ccagggtttc gtcatcaggg
      421 tgaggtgcaa ttgccaggat tttcataact taatcagagc ttttcggtgg caaataccca
      481 gcgcgagtat ttgcgcttaa agtcaggacc aagtctgctc tcggctattt cactgtagat
      541 gatcccaaaa agaccgtcaa acagggcttt tgtctcatct tcagagaaaa aatgctgcac
      601 cagtccctgc tcaaatcctt cgctcacgac aaatgtgttg ggtgctactt cttctccctc
      661 gccaaactca aagtcttccg tcgatcgcag atcacagtaa aaaagcccgc cgggtttcag
      721 cacgcgatgg acctcagtcg ctgcatttcg tgccgtcggc atcggcacat ggtccagcac
      781 cccatgagag acaacaacgt cgaacgtttc attttcgaaa ggcaattgct caatgtcgcc
      841 cacacgaaaa tcgatctcca gtccctcccg tttactccaa tccttcgccc attcaatcgc
      901 ctgtgtcgaa acgtcaaggc ctgaggcgga aaatccctgg cgagcaaaat acattgcgtt
      961 gcgaccgtta ccgcaaccga ggtcgaggac gcgctcgact ttgcgtttga cctcatagcg
     1021 atcgtgcgtc agctttcgtt gtatcaagcg cgcacaaaat cgaatgatgt cctcgtgggg
     1081 gtaccacatg gtcataaagc cttcgctgta aactttgttc cagcgtttgt gagcttcggt
     1141 ctttggtttg acggtcgcct ccatgttcga aacctctcag taagctaagt ctttgttcag
     1201 caagcgctga tcgattggcg tcgaagcgag aatctcggcc acgcgagttc ctgttcggcc
     1261 gtctccaaat ggattactac aattctcgac cagccgcctg gtcgcttcat ccgtctgaag
     1321 ttttctcacc tgtgcggcaa tctcgccccg atcatttgag acaaagaaaa cattctcgga
     1381 atgtttccgt cctgactgcc ggcgacctgc gttgatcacc ggcagatgca gaaaaggcgc
     1441 ttccagcaat ccgaggctgg aattgccgac gagcgccgct gcaccacgca acaggtttac
     1501 aaaaatatcg tcgggaatat tgtggaagat gtttatcagc ggattttgcc tgtagtcctc
     1561 aatggtccgg atcatttcaa gactgcccgg atctgaattt ggatagctca caaaagtctg
     1621 cattcgcaaa tccacgaccg cctccatcgt ctctcgcatc tgctgagcag acatatttgt
     1681 ttccgacgaa agcggatgtt gaataaccac gaggtatggt tgttcgatct tctctattcc
     1741 cagcgcactc gccaactctg tgggtcttag ttctggcgcc gttttaaagc gatccaggcc
     1801 ggcgtgccca acgttgtgca ctcgccattc ctcttcgccg gaacgaatca agcgttctcg
     1861 agcggcttca tgggtcgtta acagaatatg tgccagccgc gagacggaat gccgcaccat
     1921 gtcatcaacg ttaccaacca cgcgatcgcc ggcagcgtaa tgagcggtcg caacgttcaa
     1981 ataggcgccg cacagtgcga gcgtcatcgc ttcttcacga tcggcaggca caagcaacca
     2041 gtcaggacgt gcacgatcga caatgtgggc cagtacctgc aactgggcgg cagcgccctt
     2101 tagccgggcg gcgtcatgat cggagtggat caggctttca actctttccg tgatgggcat
     2161 tccatcacgt ccaacttctc gtacggaata gtcatgcaac ggactcagat gggctccggt
     2221 cacgatcaaa gagagctcca gctccggatg gtccatgatg gcgcggaaga tcgagcgctg
     2281 cagaaaatat tccgagcgga ttgcagtgat tcctaatact cttcgcttag ccattactgt
     2341 tccaaccttc gccaactgcc ggctgccttg attatttccc ctgcctgatc cggacagttc
     2401 aggagcaggt cgagcgtcga caggtcgggc acgaaagccg tttgccttgg tgcttgatga
     2461 taagtgatgg gtcgccactg ttggacccaa agttcgcagc caatgtggga gaagtctgcc
     2521 gggttcaagt aggcgagggc gcccgtgccg gttaaatacc tggtagcgcc cagatgtttg
     2581 cagatggaag ccacgagttc tgaagcctga ccctcaatac ccggcaactc agaagcgagt
     2641 attcgcttat tctgtacgtc cagcttcttc aacatccatt cagttgagtt gatggcaaga
     2701 tctgctaagg aggccgactc ctcttgaaac aaagcggcaa gctcgtctcg ccatcgctga
     2761 aatccttcac tgcgggaata attcgcctgc agagtcttcc agtgctttaa caacgttcgc
     2821 ccgttggcaa tcgccgtttc gcgaatgctg ctgccaaact tgtgggtgac cggaactgtt
     2881 aaccaggccg ggccatttat ggccctgatt tgattgcggt tttgcaggcc gtttttgctg
     2941 aactggaccg tatcaaggta gacgaatata tcagcagaca tgactttgtg cacataaggc
     3001 ggccagggga agtattgagg ttgatgaatg gcgaccttca cgcgctttgc cggctggttc
     3061 tagagagtga gctcacgtgc tccagcagtc gttccacaac gtaggccgcg tcttcttcac
     3121 gcatgcgggc gtggacgggc aagcagatat gacgcgacag gagctcttcc gacattggca
     3181 gcatgccggg ctgggtgccg agcatgtttt gaaagaccgg ttgtagatgg agtggaggat
     3241 cgtaagccca atcgatggta attccatcct gcgccaactg agccgcgagc actgtccgat
     3301 cgatcgtcac ggtcggcatg gcaacataac gccagtaact cggattactg cctgcttctg
     3361 caagcagagg aacaaatagc tcgcttccca gcagcaactc atcatagatc gcagcaacac
     3421 ggcgacgact ttccagaaat tccggcagac agcgcaactg actgaggccc atggccgcag
     3481 agatctcagt gaagcgattg tttctgccgg gcaagcgata gagttcagct cgctcttcca
     3541 tgtcacgacc gcggttctgc agactgcgca cggtcctggc tagatcttcc cgagctgtaa
     3601 ccagcatgcc gccctcgccg gttgtcatga tcttagtcgg atagaaagag aagcaaccag
     3661 catcagccaa actgccggcg cgctttccgc cgagctcagc gccgggagca tgcgccgcat
     3721 cctcaatcac aaatcgacca gtttcgcgca tgcgacgcac aaactcaggc agctcaggag
     3781 aaatgaaacc gccgaagtgg accacgatgg ctccgcgcgt gcgttcagta acccgcgacc
     3841 acgcatcatc gaaatccatc gaaaaggttt tcgctgaaat ctcagtgaat accggacgtg
     3901 ctcctaccag cgacaccacc attccggtag caacaaaagt ctcaaccggc accagcactt
     3961 cgtcgccggg gcctacaccc aaagcgacta aggccgcttc cattgacgaa gtgcaactgg
     4021 gaaaggcgac accgtatggc aggcgacagt aagctgcgaa ttcccgctca aactgacgaa
     4081 cgcgcggtcc catagccaac tgcccgttca atatctgcgc cagttcctgg tttagccact
     4141 cctgttccgc cggcggaaaa tacgggtacg ccgtcgttaa ggggtgtgcg ggatcaggct
     4201 tgtagttcgt tggcataaac tctcatcgat tctgtcagag acatgggctc aaagccgatg
     4261 gtgcgtcgtg cctcttcaat gtcggcataa agatcgaagc ctgatgcttc ctgttcgaca
     4321 gggattattt tgctctc
//
LOCUS       DASUEX010000330         2536 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1306368, whole genome shotgun sequence.
ACCESSION   DASUEX010000330 DASUEX010000000
VERSION     DASUEX010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1306368"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="VFV61_05565"
     CDS             complement(<1..414)
                     /locus_tag="VFV61_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899979.1"
                     /translation="MNRMTHSLVISSILLTLGAWLMNATAFAQSNTAETQLCERVVKV
                     ARPTLKRRGQPRDSQPAVYEERHIQVKCENPEAKIIQKQLENFVSLRFIGLKAVPESQ
                     AASLIRGEIPDFRSQRIPDFDVTEKGAAVLRDFLRT"
     gene            complement(427..1731)
                     /gene="glyA"
                     /locus_tag="VFV61_05570"
     CDS             complement(427..1731)
                     /gene="glyA"
                     /locus_tag="VFV61_05570"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF000586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEX4899980.1"
                     /translation="MDDLKNAPLAQVDPLIAEAIDNEVARQANGLELIASENFVSEAV
                     LEAMGSVFTNKYAEGYPKKRYYGGCEWSDVVEQAAIDRAKELFGADHANVQPHSGAQA
                     NMSVYLAAIQYGDQILGMNLSHGGHLTHGHPLNFSGISYKVADYGVSRETEQIDYDEL
                     QRIAEEHKPKLIVCGASAYPRIIDFERIGEIARSIGARMFADIAHIAGPVAADLHPSP
                     VPHADYVTTTTHKTLRGPRGGLILCKEEHAKEIDRKLFPGVQGGPLIHIIAAKAVAFG
                     EALRDDFKDYQRQVLANAKALAGELQEQGLRLVSGGTDNHLMLVDVWMDGKGTTGKEA
                     EKALEAAHITVNKNTIPFDQNKPFVASGLRIGTPAVTTRGMKEPEMREIGRLIAAIIH
                     EPESAAVQAKVKQGVADLTARFPLYPKRLKSGSSETSAMRAD"
     gene            complement(1783..2238)
                     /gene="rpiB"
                     /locus_tag="VFV61_05575"
     CDS             complement(1783..2238)
                     /gene="rpiB"
                     /locus_tag="VFV61_05575"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HEX4899981.1"
                     /translation="MKIALASDHAGFSAKEKLKSLLDELGIEVADLGTLSESSVDYPD
                     YARKVAEEVAHGRVDQGVLVCGSGTGMAMTANKVPGVRAAVAWSEETARLARQHNDAN
                     VLAIGARTTPPEEIPNIVRAWFSASFEGGRHADRVNKIESKVQSPTPKV"
BASE COUNT          525 a          708 c          680 g          623 t
ORIGIN      
        1 cgttcttagg aagtctctaa gtaccgcggc gcctttttca gttacatcaa aatcaggaat
       61 acgttgagaa cggaaatcag ggatctcccc acgaataagg cttgcagcct gcgattcggg
      121 aacggctttc agcccaataa aacgaaggct cacgaagttt tccagctgct tctgaattat
      181 ctttgcttcc gggttctcgc actttacttg gatgtgcctc tcttcataga ccgcaggttg
      241 agagtcccgt ggctgacctc gacgcttgag agttggacga gcgactttga ccaccctttc
      301 gcataactgg gtctcagcag tgttcgactg cgcaaaggcg gtggcgttca tcaaccatgc
      361 gccgagtgtc agcaggatgc tcgagataac caggctgtga gtcatgcgat tcacaaatgc
      421 tttttgtcag tcagcccgca tcgcacttgt ctctgatgaa cccgacttga gtcgtttcgg
      481 ataaagcgga aaacgggcgg ttaagtcagc gacgccctgt ttcacctttg cctggaccgc
      541 agccgattca ggttcatgga tgatggccgc gatcaggcga ccgatctccc gcatctccgg
      601 ttccttcatg ccgcgcgtgg tcacggctgg agtgccaatg cgcagccccg acgcaacgaa
      661 gggtttgttc tgatcaaagg gaattgtgtt tttgtttacc gtgatgtggg ccgcttcgag
      721 ggccttttca gcttcctttc cagtagtgcc ctttccgtcc atccaaacat cgaccagcat
      781 caggtgattg tccgtcccgc cggaaaccag ccgcagccct tgctcctgca attcgccagc
      841 gagcgcctta gcgtttgcga gtacctgccg ctgatagtct ttgaagtcgt cgcgcaaagc
      901 ctcaccaaag gcgactgctt tcgcagcgat gatgtgaatg agcggtccgc cctgaacccc
      961 agggaacaat ttccgatcga tttcctttgc gtgttcttct ttacagagaa tcaagccgcc
     1021 gcgcggccca cgcaaagtct tatgcgttgt cgtggtcacg taatcggcgt gcggcacggg
     1081 agacggatgc aaatctgcag cgactggtcc ggcgatgtgg gcgatgtcgg caaacatgcg
     1141 cgcgccaata ctgcgtgcaa tctcgccgat gcgttcaaaa tcaattatgc gcggataggc
     1201 ggaggccccg cagacgatca actttggttt gtgttcttca gcgatccgct gcagttcgtc
     1261 gtaatcgatc tgctcggttt cgcgagagac cccgtaatcg gccactttgt agctgattcc
     1321 ggaaaagttc agcgggtgac catgagtgag atggccgccg tgagataggt tcattccgag
     1381 gatctgatcg ccgtactgga ttgccgccag ataaaccgac atgtttgcct gggcgccgct
     1441 atggggttgc acgttggcgt ggtcggcacc aaacagttct ttcgcgcgat cgatggctgc
     1501 ctgctcgacc acatcgctcc attcgcagcc gccgtaataa cgcttcttcg gataaccctc
     1561 ggcgtactta ttggtaaaga ccgagcccat cgcttcgagc acggcttcgc tgacaaaatt
     1621 ttcggaagca atcaactcga gtccattcgc ctggcgggcg acttcgttat cgatcgcctc
     1681 ggcgattaag ggatccactt gagcgagggg agcatttttc agatcgtcca tcatgttcct
     1741 cttgaatttg gtctttggtc ttttaacttt gaactttgaa cctcaaactt tgggcgttgg
     1801 actttggacc ttggactcaa tcttatttac ccggtctgca tgtcgtccgc cttcaaatga
     1861 cgcactaaac cacgcgcgca caatgttcgg aatctcttcc ggcggagtgg tgcgggcgcc
     1921 gattgccagc acgtttgcat cgttgtgctg gcgggcaagt cgagccgttt cttctgacca
     1981 ggccacggct gcgcgtacgc cagggacttt gttagcggtc attgccatac ccgttccgga
     2041 gccgcacacc aaaacacctt gatcgactcg gccgtgggca acttcctcgg cgaccttacg
     2101 agcgtaatcc gggtaatcga cagatgactc agaaagcgtt cccaagtccg ccacttcgat
     2161 tcccaattca tcgagcagcg acttgagttt ctcctttgcg gaaaagcctg cgtggtcact
     2221 ggcaagcgca atcttcacgt attcaacctc atggaaaaga attagccgaa gaggacgtcg
     2281 atgttacaac acacgacgga agttgtcagt tgttatggag tgcgacggca aagcgaagtc
     2341 ccgacgcgtc gggaacggcg ctttggttgc tcaccaagtg gagtcagcta atcgaaagcg
     2401 gtgtctcccg gtgcatcggg attgccaccg cactccaaaa cgcctgtgaa ttgctcaacg
     2461 cttgctggtc taagttacaa tcttcgcatg ccaaactatc tcgatgtctc cacttgggcg
     2521 cgacgcgacc tgtttg
//
LOCUS       DASUEX010000331         3739 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1558205, whole genome shotgun sequence.
ACCESSION   DASUEX010000331 DASUEX010000000
VERSION     DASUEX010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3739
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1558205"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1588
                     /locus_tag="VFV61_05580"
     CDS             <1..1588
                     /locus_tag="VFV61_05580"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TRCF domain-containing protein"
                     /protein_id="HEX4899982.1"
                     /translation="KRDMEEPTPMDRLLCGDVGYGKTEVAMRAAFKAVMEGKQTGILT
                     PTTVLAYQHYDTLRARFAPFPVKVELLSRFRSAKEQKEIVKRVESGEVDVVIGTHRLL
                     SKDVRFKDLGLVVVDEEQRFGVAHKEKLKQLKKRVDVLTLSATPIPRTLNMSLTGLRD
                     MSLIETPPRDRLAIQTQVVQFSENVIKSAIELELARGGQVFLIHNRVETIETIAALVQ
                     RIVPQARLVVGHGKMNEKEMEQVMLDFIEHKYDVLVATTIIENGIDIPRANTIIINRA
                     DTYGLSQLYQLRGRVGRSSRRAYAYLLIPSEQELTPIARRRLAAIREFSDLGAGFRIA
                     ALDLELRGAGNFLGGQQSGHMDALGFDLYTQMLERTVAELRGEQVEDETSVSINLGVN
                     VAIPEDYIQDMGQRLRTYKRVSSSRDEETLANIRRETEDRYGRVPQPVEDLFAYARLR
                     RLAEEIGVLSIDKTAEGIAIKFSENARISPEKLTEFVSRGRNSTFTPAGVLRINLEDA
                     QADNELQVAREALLELRLGD"
     gene            1656..2762
                     /locus_tag="VFV61_05585"
     CDS             1656..2762
                     /locus_tag="VFV61_05585"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEX4899983.1"
                     /translation="MRAKLTVLSAALVSACALFMLAAPAVKAQEAELTVVDEVIAQVN
                     EDVITLSMLKRESKERIETLKQNGMNAQQATEEVAKRKAELIATLINERLLLQKGKEL
                     ELTTDVEAEVNRRMLDVAKEQGITTIEKLDQAMKESGLDPVLTRQTLRNEIMKQAVFQ
                     QEVDRKIYFSATMDDLKKYFEANRDKFLKPENVVISEIYLTFAGKNEADVKAKALELV
                     AQLRAGADFGSVAATNSERELNGVRVAPQNKGKVGRFETPQLREDIAVAIKNVKVGGI
                     SEPLRGTEGYQIFRVDERNPGSTSATFNEEQVRQAITVERAPKERDAYLQTLRNDAYV
                     NVAKDYQESVLPFLKISQEAAASDNAKPATKKKS"
     gene            2829..3113
                     /locus_tag="VFV61_05590"
     CDS             2829..3113
                     /locus_tag="VFV61_05590"
                     /inference="COORDINATES: protein motif:HMM:NF013633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEX4899984.1"
                     /translation="MRLDLFLKSSRLCLRRTVAQKMCDAGLVSVNGKVAKAATTVKAG
                     DELSLRRRERITTIRVLALPAARQTSRSEATSLYEIIGEVGVADEGGNSV"
BASE COUNT          936 a          931 c         1075 g          797 t
ORIGIN      
        1 caaacgcgat atggaggagc cgaccccgat ggaccgtcta ctttgtggcg acgtcggtta
       61 tggcaaaact gaagtagcga tgcgcgccgc ctttaaagcc gtaatggaag gaaagcagac
      121 tggcatcctg acgcccacaa ccgtcctcgc ctatcagcac tacgacacgt tgcgcgcacg
      181 ctttgccccg tttcctgtga aggtggaatt gctatcgcga tttcgctccg ctaaggagca
      241 aaaagaaatc gtcaagcggg tggagtcggg cgaagttgat gtagtaatcg gcacgcaccg
      301 actactttcc aaggacgtgc gcttcaaaga tcttggcctg gtcgttgtcg atgaagagca
      361 aagattcgga gtggcgcaca aagagaagtt gaagcaactg aaaaagcgag tagacgtcct
      421 gacgctttcg gcaacgccca tcccccgtac cctgaacatg tctttgactg gtttgcgcga
      481 tatgtcgttg atcgagactc cgccgcgaga tcgcctcgct attcaaaccc aggtagttca
      541 gttttcggag aacgtcatca agtcggcgat tgaactggaa ctggcgcgcg gtggccaggt
      601 ttttctgatt cataaccggg tggaaaccat cgaaacgatt gccgcactcg tgcagcggat
      661 cgtaccccag gctcgcctgg tggttggtca tggcaaaatg aacgagaagg aaatggagca
      721 ggtgatgctc gattttattg agcataaata tgatgttctg gttgcaacta ccatcatcga
      781 gaacggaata gacatccccc gcgccaacac gatcatcata aaccgcgccg atacttacgg
      841 tctttctcag ctttatcagc tacgcggccg agtcggtcgt tccagcagac gggcctatgc
      901 ttatctgctg attccgagcg agcaggagct tacgccgata gctcgcaggc gactcgcggc
      961 aattcgagag ttctcggacc tgggcgctgg ttttcggatc gcggcacttg atctcgagtt
     1021 gcgcggggcc ggcaattttc ttggtggcca gcaatcaggc cacatggacg ccctgggctt
     1081 cgatctttac acgcagatgt tggaacgcac cgtagccgag ttgcgcggcg agcaggttga
     1141 agacgaaacc agcgtttcga tcaacctggg tgtcaacgta gcgattcctg aggattacat
     1201 tcaggacatg ggccagcgtt tgcgcactta taagcgagtc tcctcatccc gcgacgaaga
     1261 gacgctcgcc aacattcgcc gtgaaacaga ggatcgctac gggcgcgttc cccagccagt
     1321 ggaagactta tttgcctatg cacgactgcg gcgcctggcg gaagagattg gggtgctttc
     1381 gatagacaaa actgcggaag gaattgcgat caagttcagt gaaaatgcca gaatttcacc
     1441 ggaaaagctg actgaatttg tcagccgcgg tcggaactcg acctttacac ccgccggcgt
     1501 tttgcgcatc aatctggaag acgcgcaggc tgacaacgaa ttgcaggtgg cgcgtgaggc
     1561 attgctcgaa cttcgcttgg gcgattagaa tcgggccctt gtaaaaagtt aaccagaacg
     1621 aataattcaa ccagcgatat ataggagtac ttttcgtgag agcaaaatta actgttttaa
     1681 gtgcggcgct tgtttccgct tgcgctttat tcatgcttgc ggcacctgcg gtcaaggcgc
     1741 aggaggctga gctgactgtc gtcgatgaag tgattgccca ggtgaacgaa gacgttatta
     1801 cactttcgat gcttaaacgc gaaagcaaag aacggattga gacactgaag cagaacggca
     1861 tgaacgcgca acaggcgacg gaagaggtag ccaaacgaaa agccgaactg atcgccactc
     1921 tcatcaacga gcggctcctg ttgcaaaaag gcaaagagct tgaactaaca accgacgtgg
     1981 aagcggaagt gaaccgccgc atgctggatg ttgctaagga gcagggcatt accaccattg
     2041 aaaagctcga ccaggcgatg aaagaaagtg gactcgatcc agtgctaacg cgccagaccc
     2101 tgcgcaacga aatcatgaag caggcggtat tccagcagga agttgatcgc aagatctatt
     2161 tcagcgccac gatggacgac ctcaaaaagt attttgaagc taatcgcgac aagttcctta
     2221 agccagagaa cgtggtcatt tcggagatct atttgacgtt cgctggtaag aacgaagctg
     2281 acgttaaagc aaaggcgctg gaattagtgg cgcagctccg tgcgggcgcg gatttcggct
     2341 cagtagccgc gaccaactca gaacgcgaat tgaatggcgt gcgcgtcgcg ccccagaaca
     2401 agggaaaggt cggcaggttt gaaacaccgc agttacgcga agacattgcg gtggcaatca
     2461 agaacgtcaa ggtgggtggg atcagcgagc cgcttagagg caccgaggga taccaaattt
     2521 ttcgcgtgga cgaacgcaac cctggttcga ccagcgcgac gttcaatgaa gagcaagtgc
     2581 ggcaggcgat aactgtcgag cgcgcaccaa aagagcgtga cgcttacctg caaacgttgc
     2641 gcaacgacgc ctacgtgaat gttgcgaaag actatcagga atcagttctg cctttcctca
     2701 agatttctca ggaagctgcc gcgagtgata acgccaaacc ggcaaccaaa aagaagagct
     2761 aagcttgaac tgacattttt cgaaacccgg gccgtggcat cgagccggcc cgggtttttt
     2821 agttgccgat gaggttagat ctctttctca agagcagccg gctttgcctg cgccgtacgg
     2881 tggcgcaaaa aatgtgtgat gcaggtttgg tttcagttaa tggcaaagtc gcaaaagccg
     2941 ccaccacagt taaagccggc gatgaattgt cgttgcgccg gcgcgagcga ataacaacaa
     3001 tacgggtgtt agcgttgcct gctgctcgcc agacctcgcg cagtgaggcg acttcattgt
     3061 atgaaatcat tggcgaagtc ggcgtagccg acgaaggcgg taatagcgtc tgacttgtcg
     3121 cagttgagat ttcgacgcgg agcgtcgtaa tgactttagc ccggcgtttt aacgccggga
     3181 ttgcgacgac tcattgaggt cgtcgcgtag cgacgattca aacgtcgctg acgcgacgca
     3241 aggcggtgca tctcatgtcg ctaacccggc tttaaaaagc cgggctaaaa tccagccgcc
     3301 gttgcgcggc gcggcaaccc acagcgctgc gggttgatta catccatgga aatggccgaa
     3361 ggagagattg gacatgaggc ggtgtcgcat tggagaatga agacattgag gagctgtatg
     3421 atgcggttgc gttaggaacg cccggtagta gggttcaccg gacagaacgt ctttgccaag
     3481 tacgaagcag aattcacaaa gctcctcgac ggtctcgaga tttattgaac gggtaaacaa
     3541 taggtttagg ttcacagagt ttcatgatta aacccttcaa gattcgcgac gtggtgatca
     3601 ctccgccatt aattctgtcg cccatggctg gcgtcaccga tgtgtcgttt cgccggttgt
     3661 tgaagcgctg tggcggtgta ggtttgacgg tttccgaatt catctccgtt gaggggctga
     3721 cccggaacaa tcccaagtc
//
LOCUS       DASUEX010000332         3405 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_633998, whole genome shotgun sequence.
ACCESSION   DASUEX010000332 DASUEX010000000
VERSION     DASUEX010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3405
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_633998"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..689
                     /locus_tag="VFV61_05595"
     CDS             <1..689
                     /locus_tag="VFV61_05595"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX4899985.1"
                     /translation="GEDGTRSRDFAVEQIKTAGANLLGQSLGLVGNIIGAIVKAFFVI
                     ITMYYLFRDGDRIVLALPGVLPLSNNQSEAILARIRQVVSASVYGVVTIAMIQGLLGG
                     LAFWILGVPSPVLWGTLLAFVCMIPIAGSFMVWLPAAIYLVVTGHWTKAILLTIWGAL
                     VISTIDNFLRPKLISNQTKLHELLVLFSVLGGISVFGLLGIVLGPVVLAITLGLLETF
                     KSDKPFSIAE"
     gene            1009..2949
                     /locus_tag="VFV61_05600"
     CDS             1009..2949
                     /locus_tag="VFV61_05600"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX4899986.1"
                     /translation="MRKSSCRPWIALLLALTFFAGLLISDHAHAESSPKAKPAHKIST
                     DVLRKVNGGQGNERVRVIVQPRQTWNSDLDSALQGADVTNIRQFQKFNFRVLTVSASA
                     AAALAARDDVAFVSVNREVRTLGHVSLTTGADAVRETNGTTTGGLDGTGIGIAVLDSG
                     IDTDHKAFLDKSNNLRVVVDRDFTGESRTDDPYGHGTHVASMAAGNGRISNAQYTGIA
                     PNANIINLRVLNSQGAGTISGLLQALDWVLTNRATYNIRVVNMSLGTPAVDSYRYDPV
                     CLAVRHLVDAGIVVAAAAGNNGKDSAGNKIYGRIHSPGNEPSAITVGAANTFGTNARN
                     DDGVTSYSSRGPTRSYWTDSENVKHYDNLVKPDLIAPGNKIVQAQALDNLLVANNPQL
                     DAGVSPVDNRRMMYLNGTSMATPAVAGAAALMLQANPTLTPNLVKMILQYTAQPLAGY
                     NSFEQGTGELNIEGAIRLAKLVRRDLSLATEVGEPLLGTNPLPLPQTTIAGHTFSWSQ
                     GILRNFTWTIGEALIAKYQSIYGIGASFGGVITGDGVITGDGVITGDGVITGDCIMTS
                     NGVITGDGLVFLQVSTLLNGSTLLSGMVLGDGVITGDGVITGDGVITGDLTLQAQSAT
                     LGGDETSSMDVMIDEGVDFLDY"
     gene            3062..>3405
                     /locus_tag="VFV61_05605"
     CDS             3062..>3405
                     /locus_tag="VFV61_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899987.1"
                     /translation="MEVRPLTVGQEAEVLEFLAAQPLHNVYMAGFIRDNGMVSPLNRG
                     TFYACRDDAGRLEGVALIGHVTQFDAQTERAMNSFVRAAQACPSVHVVMGDSAMVRDF
                     WHKYSSRGQELRV"
BASE COUNT          754 a          931 c          947 g          773 t
ORIGIN      
        1 tgggcgaaga cgggacgcgt tcgcgcgact ttgctgtcga acaaataaaa actgccggag
       61 ccaatctcct gggtcaatct ctcgggctcg tgggcaacat catcggcgcg atcgtgaaag
      121 cattcttcgt gatcatcacg atgtattact tgttccgcga tggcgatcga attgtgctgg
      181 cgctacccgg agtattgcct ctcagtaaca atcaaagcga agcgatactt gcgcgcatca
      241 ggcaggttgt gagcgcgagt gtctatggtg tggtaaccat tgccatgatt cagggtttgc
      301 tgggaggact ggctttttgg atcttgggcg ttccttctcc ggtcctgtgg gggaccttgc
      361 tcgcgttcgt ctgcatgatt ccgattgccg gctctttcat ggtctggctc ccggcagcaa
      421 tctatctggt ggttactggt cattggacca aggcgatcct actcactatc tggggagcgc
      481 tggtgatcag cacaatcgat aactttttgc ggccgaagct gattagtaac caaaccaagc
      541 tgcacgagct cttagtcctc ttttcggttc ttggtggcat cagtgtattc gggttgcttg
      601 gtattgtatt gggaccggtt gtcctggcaa tcacccttgg tcttctcgaa acattcaaat
      661 ccgacaagcc attttcaatt gccgagtgac gatggcggat ttccgattgg cttctttcgc
      721 tgcgggctaa ttggcaatcg gcaattgcca atcgacattc ccaagcccac cggctaccac
      781 ctagtgaccg accgacctca actagtcgtc aatcgacgat tttttctgtt aaacaagttt
      841 gcaatcacct ggttctttgc tagaatccat ggcctccaga aatccccccc gactctggta
      901 ataaaacatt ggcttccgag aattactgcg cagcctgcgg actacctctc ggctcatcgg
      961 gcttctagaa ggctagattc acgccaaatc caggtgaggg caccaaaaat gaggaaatcc
     1021 tcgtgtagac cgtggatagc gctgttgctc gcgctaacct tcttcgccgg cctgctaatc
     1081 agtgatcacg ctcacgcgga aagcagtcca aaagcaaagc ccgctcataa aatctcaacg
     1141 gatgtcctga ggaaggtaaa tggcgggcag ggaaatgagc gcgtccgcgt catcgtccag
     1201 ccgcgccaaa cgtggaactc cgacctggac tcggcccttc aaggggctga cgtaaccaac
     1261 atacgtcaat tccagaagtt caatttccgc gtgctcacgg tttcagcctc tgccgccgcg
     1321 gcgctggctg cgagggacga tgtcgcattc gtctccgtaa accgtgaggt gcgcacgctg
     1381 ggtcacgtct cactaacgac cggagccgac gctgtgcgtg agacgaacgg tacgactacc
     1441 ggtggcttgg atgggaccgg gattggcata gccgtactgg actcagggat tgataccgat
     1501 cacaaggcgt ttctggacaa gagcaataac ctccgggttg ttgtcgacag agatttcact
     1561 ggcgagtcgc gcactgacga tccctacggt cacggcactc atgtggcttc gatggctgca
     1621 ggcaacggtc gcatctcaaa cgctcagtac accggcatcg cacccaacgc aaatatcatc
     1681 aacctgcgcg tgctgaactc gcagggagct gggacaatta gtggtctgct ccaggcactc
     1741 gactgggtgc tgacaaaccg cgctacctac aacatccggg tcgtaaacat gagtctcggt
     1801 acgccggctg tagattctta cagatacgat cccgtgtgcc tggcggtgcg tcatctggta
     1861 gacgcaggca tagtagtcgc ggccgcggcc ggtaataatg ggaaagacag cgcgggcaat
     1921 aagatttacg ggcgcattca ttcaccgggt aatgagccct cagcaatcac cgtgggtgca
     1981 gccaatacct tcggaaccaa cgcgcgaaat gatgatggcg ttacttctta cagctcccgc
     2041 ggcccaacgc gcagctattg gacggacagc gagaatgtca aacattacga caacctggtt
     2101 aagcccgatc tgattgctcc aggaaacaag atcgtccagg ctcaggccct ggataacctg
     2161 ctcgtcgcaa acaatcccca actcgatgcc ggggtgagcc ccgtcgataa tcgccgcatg
     2221 atgtacctaa acggcacttc gatggctact cctgcggtgg cgggcgctgc cgcactaatg
     2281 cttcaggcca atcccactct tactcccaac ctcgtcaaga tgatccttca gtacacagct
     2341 cagccacttg ctggatataa cagtttcgag caaggcacag gcgaactaaa tatcgaaggc
     2401 gcgattagac tcgccaaact tgtgcgtcgc gatttgtcat tagcaactga ggtgggcgag
     2461 ccgttgctgg gaacgaatcc gctccccctc ccacaaacaa ccatcgcggg ccataccttc
     2521 tcgtggtcgc aagggattct gcggaatttc acctggacga ttggcgaggc actgattgct
     2581 aagtatcaaa gtatttatgg catcggcgct tcattcggtg gagtcatcac gggagatggt
     2641 gtcattactg gtgacggagt gatcaccgga gacggtgtga tcaccggcga ctgcatcatg
     2701 accagtaacg gggtcatcac tggcgatggc ttggtcttcc ttcaggtaag tacgttgctc
     2761 aacgggagta ccttgctcag cggcatggtc cttggcgatg gagttatcac aggcgacgga
     2821 gtcattaccg gcgacggagt catcaccggt gatttaactc tgcaggcgca atccgcaacg
     2881 cttggcggag acgaaacgtc atcgatggat gtgatgatcg atgagggcgt agatttcctg
     2941 gattattgac agcgagcatt tctcaatcct agccacgggg ttcaagctaa cggtgcaacc
     3001 tcttagccgt ctaagacagg caagtatgca agccgcacct gggcggactg cgtcaagtcc
     3061 tttggaagtt cgccctctca ctgtggggca ggaagccgaa gtgctggaat tccttgcggc
     3121 gcagccactg cataatgtct acatggcggg cttcatccga gacaacggaa tggtgagccc
     3181 gctcaatcgc ggcacattct acgcttgccg cgatgacgcc gggcgactcg agggtgtcgc
     3241 cttgatcggc cacgtcacgc agttcgatgc gcagacggaa agggccatga actcatttgt
     3301 ccgggctgcg caagcgtgtc cctcggttca tgtcgtgatg ggcgacagcg caatggtgcg
     3361 agatttttgg cataagtact catcacgcgg gcaggaattg cgggt
//
LOCUS       DASUEX010000333         2475 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1306505, whole genome shotgun sequence.
ACCESSION   DASUEX010000333 DASUEX010000000
VERSION     DASUEX010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1306505"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="VFV61_05610"
     CDS             complement(<1..455)
                     /locus_tag="VFV61_05610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899988.1"
                     /translation="MLDIFKATVMGVLLLAVMALGTSSAKADPVTFSTSGTFTCAGCA
                     GSGTNSVTFLGGSGNALMITFTGLGSTSLNTPTGTSFGNFQTFFSGTGTIAASGTFTL
                     TITQTVPIAGSDSFSATFTGLFSASNSGSGVVNFTVTSVNIGGITYSIT"
     gene            1242..>2475
                     /locus_tag="VFV61_05615"
     CDS             1242..>2475
                     /locus_tag="VFV61_05615"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX4899989.1"
                     /translation="MRRPSLSFFTTLPLILFAVSVAPAQSQLTADIGEKIDKLAVETL
                     TRTGVPSASIAVVRDGQIVYVKAYGDAKLEPKTPATAAMRYSIGSISKQFTASAILLL
                     QEQGKLSLDDKVGKYIPDLTRANEVTIRQLLSHTSGYQDYWPQDYVMKPMLSPTTAQK
                     IMDTWAKKPLDFEPGTKWQYSNTNYVIAGAIVEKVARMPFLRFMQEKIFTPLGMKSVM
                     SIDEGRLTESDPTGYKRYALGPLRVAPKEGTGWLFAAGELAMTPEDLAKWNVSIIEGK
                     LMKPSSYLELGREVLLKNGVGTNYGLGVSVGMPNGRRALSHGGEVSGFTSQSIVFPDD
                     RVAVVVLTNQDAAGAAGPIASGIPPLLFATNDSATAVKLEQAKKIFEGLQQGTIDRSL
                     FTENANGYFSEQALEDFKN"
BASE COUNT          633 a          621 c          673 g          548 t
ORIGIN      
        1 ttcgtgatgg agtaagtaat tccgccgatg ttcaccgagg ttacagtgaa attcaccact
       61 cctgacccgg aattacttgc cgaaaacaac ccggtaaagg tcgccgagaa ggagtcactc
      121 ccagcgatcg gaacggtttg cgttatggtc agcgtgaacg ttcccgatgc ggcaatagta
      181 ccagtacccg aaaagaacgt ttggaaatta ccaaaactgg tacccgtagg tgtgttcaag
      241 ctggtagatc ccagaccggt gaaagtgatc atgagcgcgt tgccactacc gcccaaaaaa
      301 gtgacagagt tggtgccgct tcctgcgcaa cctgcgcacg tgaaagtacc ggacgtagag
      361 aaggttactg gatcggcttt tgcgctggac gtgcccaatg ccatcactgc gagcaggagc
      421 acacccatca cagtcgcttt gaaaatatct agcatatttg tttcctcttt gcgtgagctt
      481 cgatcagaac gtccgcacgc agccctgacg agagaatcgg tgaacctacc accttcttgt
      541 tcagtcttcg gctgcggggc aagggcagcg ttggatattt ttcgagttgc tgggtcttac
      601 aacagctcgg cagccgtagg agacaagcgt ccgcggctgg attggttggg agcaagtata
      661 cgcgcgctca aaccaatcag ccacgattga caggtcgaaa ttacagtggc acttgcatca
      721 accccagcaa atcccaggcc taagaggaga gtgttaagtt ccttaggtaa aacccgaatc
      781 ttgattgttg ataccgccgg caactgtctg atattgagat tgagagtctg ggctttggac
      841 gccaaggttc tgagcttaaa gtgaggctgg ggaataccat ggcggtactc gttgtcggag
      901 agtatatatg tcccagtgat tgggaggacg ttattaggag tcgacgacgc cggagctcgg
      961 caagcgtttg cacgagatca cggtcgaccc taagacagtg cagaagcccg cttcgcttgc
     1021 cacaaaacag tgacggcaaa gattgcgttt cgaaatactc tcaagcatcg cagtgtgcaa
     1081 aaacaaaaac agccgccttt cagcggctgt ttttgctggt agatagtggt gagtggaaag
     1141 cgccttaccc agtctgcgcc gtctggctga gctataatta cgcgtcttaa actacgctac
     1201 ttcaattacg ctgcttcaac cataacctaa aaggaggccc agtgcgacgc ccaagccttt
     1261 catttttcac aacccttcct ctcattctct ttgctgtttc agttgcgcct gcacaatcgc
     1321 aacttaccgc tgatatcgga gaaaaaatcg acaagctcgc agttgaaact ctaacgcgaa
     1381 caggtgtgcc tagtgcttcg attgccgtcg tgagagatgg acaaatcgtc tatgtcaaag
     1441 cttatggcga tgcgaagctc gaacctaaaa cgcccgcgac tgcggccatg cgctacagca
     1501 ttggctcgat tagcaaacag ttcacagcct cggcgatttt gctattgcag gagcaaggaa
     1561 aactgtctct cgatgacaaa gtcgggaagt acattccgga tctgacacgc gccaacgaag
     1621 tcacaattcg gcagctgctt tcacatacct caggttatca agactactgg ccacaggatt
     1681 acgtcatgaa gccgatgctg tcgccaacga cggcgcagaa gatcatggac acatgggcaa
     1741 agaagccact cgatttcgag cccggcacga aatggcaata cagcaatacg aattatgtga
     1801 tcgccggtgc aattgtcgaa aaagtcgcgc gcatgccgtt cttgcggttt atgcaggaga
     1861 agattttcac gccgctaggc atgaaaagcg tcatgagtat cgatgaaggc agactaacgg
     1921 aaagcgaccc cacgggctac aaacggtacg cgcttggccc gctgcgtgta gctccgaagg
     1981 aagggacggg ttggttgttc gcggccggcg aattggccat gactcccgag gaccttgcga
     2041 agtggaatgt atcgatcatc gaaggaaagc tgatgaagcc tagctcctac ctggaactgg
     2101 ggagagaagt gcttctaaag aacggagtcg gaacgaatta tggactaggt gtcagcgtgg
     2161 gtatgccaaa cggccgtcgc gcgctgtcgc atggcggcga agtctccggc ttcacctcgc
     2221 agagcatcgt ttttccggat gatcgggttg cagttgttgt gctcaccaac caagacgcgg
     2281 ctggtgctgc aggccccatc gcttcaggta ttcctcccct gctgttcgca actaacgatt
     2341 ctgcaacggc ggtgaagctg gagcaggcga agaagatatt cgaagggttg cagcaaggaa
     2401 caattgaccg ctcgctattt actgaaaacg cgaacgggta cttcagtgaa caagcgttgg
     2461 aagattttaa gaaca
//
LOCUS       DASUEX010000334         4289 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1558394, whole genome shotgun sequence.
ACCESSION   DASUEX010000334 DASUEX010000000
VERSION     DASUEX010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4289
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1558394"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            293..4132
                     /locus_tag="VFV61_05620"
     CDS             293..4132
                     /locus_tag="VFV61_05620"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX4899990.1"
                     /translation="MMIPSSRQWVYASIRLTFSLVIAVFCAQIALGQAQSNAADLQGF
                     VRDPSGAVVVGALVSVRNAATNFSREVTTNDDGYYQITNLPPGSYEITVEAAGFAKGR
                     IASVTLTVGQRADLDIPLTVGEVGATVDISAAEVALIESSSTTVSNTIDQMRINNLPI
                     NERSATGFALTISTVGRDNGRPIGPAPTSGLNIGGQRGRSTLVQVDGADYTDNSINAA
                     RSTVSQEGVQEYQVSTNSYAPEFGRATGGIVNVVTKSGTNDLRGNVFGFLRHKSIQSR
                     NPFAPVIDNDPSKRPPFTRVQYGATLGGPLDRDRTFFFTSFEQRRRQESGFFTGNVGA
                     GLTSSLTIPIPGLGNQTFSNLTPAQAAFIGGELASGDAGRANRAISYAYFASTGGQIG
                     LNGVSTLRSPGGSIPAGQIIGGRFFLTGTPVPTLANNGILPNFVTVNANGQPIAFRPL
                     NSLSRIFPISEDTTFFSTRIDHQITDKHQFTMRFGYNPTELTGIQDESQNQTVGQNDY
                     SRTGLQKIKDASFVTTLASTLSNRMVNEFRFNFGQRKAFFDSQIPGTAIQIIGAAFLG
                     SNPFSPVDRRETRYQFTDNLNWIHQTHTFKVGGDYSFLDINAQFQLNFPGLFNFSGVP
                     GALSQRFLDGTVSTQAGCVVAPPTPVPAYCTPPDLSPVQSYGFGVPSVFIQGFGNPNS
                     SIKNRPIAFFAQDSWKLRPNFTLNYGVRYDVELTDTIAPIGFTDPLTRITLSAADLQR
                     AQDVVGVQQGFPRDKNNWAPRVGIAWDIKGDSKTVVRAAFGMFYDHPLLAVAFNSDIA
                     DAAQQQQYFNVLPGSPAPNQLLNPLQIFQGTVCSPASGNPICAPLGAGFTTPGVATTA
                     QYLPGRVRFNDQTFVGFGPIFPFTLAVDKNFEYASANQGNFTIERQLTSNMSFSGSYI
                     FVGAHHLPHPQDINAPQQNLLIENFRRFAGRNPASNSEAYLFSIPNACPGAGCPPGFT
                     VIIPGLVGRNGLGQGVVNPIVANFFRPNAPNYFFANSITGGLVTKAVLDAALAASNSL
                     RTPGSLTPFGEIDAQLSDGNSTYHAMNLEVKRRFSNNFQFLASYTWSHSIDDSSDLQT
                     LLKPQDNRNFRAERADSLFDQRHRFVFSGALTSPASWRGSSSAAHRFLADFTIAPIFE
                     MSSGRPFNILTGVDSNGDLQGSTDRPAVAADGTLFIPTTAFTTGSLGRNLGITHRYAS
                     LDLRVMKAIPLGERVRLDLIAEGFNLFNRFNEAAASPLFSDVNAFRQRAGNGRYFSNP
                     TASYDPRQFQFGLKLNF"
BASE COUNT          927 a         1246 c         1096 g         1020 t
ORIGIN      
        1 ccttaaagcc ccgtaactgt cgttagatcg cgcacttaat aagccttgcc accggcgcgc
       61 ttttggggta aaaagacgga gcgttattgc ttagtactct tggcaccttt tctccagggg
      121 ggcctggctc aggtcagagg ctattggccg atcactcccc aacacgccgt gaaagattaa
      181 ggaaggggtg atctggtttc cgcagatacc aggagcactg tcgtccactc accgagataa
      241 ttgcacaatc ggaattcgcg agtgcggatt ctaaaagttg aaggagaata agatgatgat
      301 cccgtcctcc aggcagtggg tttacgcctc gattcggctc actttctctt tagtcattgc
      361 cgttttctgc gcacagatcg ctttaggcca ggcgcaatca aatgctgccg acttgcaggg
      421 ctttgtgcgt gacccgtcgg gagctgtcgt agtcggcgct ctcgtctcgg tgcgcaatgc
      481 ggcgacgaac ttctcccgtg aggtgaccac gaacgacgac ggttactatc agattaccaa
      541 tctgccgcct ggaagttatg aaataacggt ggaagcggcg ggttttgcca aaggaaggat
      601 tgcgagcgtg actttgaccg tgggccaacg agcggatttg gatattccgc ttacggtcgg
      661 tgaggttggg gcaacggtcg atatctctgc cgctgaggtt gcactgatcg agtcgtcaag
      721 caccacagtc tccaacacta tcgatcagat gcggatcaac aatcttccga tcaacgagcg
      781 gagcgcaact ggattcgctt taacaatatc cacggtcggc cgcgacaacg gacgtccgat
      841 cggaccagca ccaacttcag gcttgaatat tggcggccag cgcgggcgct caactctcgt
      901 tcaggtagat ggcgcggact ataccgacaa ctccataaac gctgctcgca gcaccgttag
      961 ccaggaaggc gtgcaggagt accaggtctc aactaattct tatgctcccg agtttgggcg
     1021 tgcgactggc ggcattgtga acgtggtgac caagagcggt accaacgatc tgcgcggaaa
     1081 cgtttttggg tttctgcggc acaaatcaat ccaatctcga aatccctttg cgccggtcat
     1141 agataacgac ccgagcaaac gaccgccatt tacacgagtc caatacggcg ccaccctcgg
     1201 tgggccactg gatcgagatc ggaccttctt ctttaccagt ttcgaacaac gtcgtcggca
     1261 ggagtcgggt ttcttcaccg gcaatgtcgg tgcaggtctg accagcagtc tcaccattcc
     1321 gataccgggc ctcggaaacc aaacgttttc gaatttgact ccagcacaag cagcgttcat
     1381 tggaggagag ctggcgagcg gtgatgcggg acgagccaat cgcgcgatat cttatgcgta
     1441 ctttgcctct acgggtggac agattggcct taatggcgtc agcacgctgc gcagtcccgg
     1501 tgggtccatc cctgcgggtc aaatcatcgg cggacgtttc tttctgactg gcacaccggt
     1561 gcctacgctg gcgaacaatg ggattctgcc gaactttgta acagtcaacg cgaatggcca
     1621 gcccatcgct tttcggcctc tcaacagtct gtcgcgcatc tttccgatct cggaagacac
     1681 aactttcttt tcgacgcgca tagaccatca gatcactgac aaacaccagt tcacgatgag
     1741 gttcggctac aacccaactg agctaaccgg cattcaggat gaatcgcaga accaaaccgt
     1801 cgggcaaaat gattattcgc gaacaggcct tcagaagata aaagatgcgt cattcgtaac
     1861 cactttggcg tcgacgcttt caaaccggat ggtgaacgag ttccgcttta actttggaca
     1921 gcgcaaggct ttcttcgatt cacagattcc cggcacggcg atccagatca ttggtgcggc
     1981 cttcctcggc tcaaatccct tctctcctgt cgatcgccga gagactcgtt atcaatttac
     2041 cgataacctg aactggattc atcaaaccca cacgttcaag gtcggtggtg attacagctt
     2101 cctcgatatc aacgcgcaat tccagttgaa ctttccgggt ctctttaact tcagcggggt
     2161 gcccggtgcc ctgtcgcaaa gattcctgga tgggaccgta tcaactcagg cgggatgtgt
     2221 cgtagcccct ccgactccag ttccggcata ttgcacaccg cccgatttgt cgcctgttca
     2281 gtcttacggc tttggtgttc cctcagtgtt tatccagggc ttcggcaacc caaacagttc
     2341 gatcaagaac cggccaattg ctttcttcgc gcaagattca tggaagcttc gtcctaactt
     2401 caccttgaat tatggtgtcc gctacgacgt agagctgact gacactattg cgccaatcgg
     2461 attcaccgat ccgttgacgc ggattacgct tagcgctgct gacttgcagc gagcccagga
     2521 tgtggttggc gtgcagcaag ggttcccacg cgataagaac aactgggcgc ctcgcgtcgg
     2581 catcgcctgg gacatcaagg gcgacagcaa gacagtcgtt cgtgcagctt tcggtatgtt
     2641 ctacgatcac ccgctgctcg ccgttgcctt caactccgac atcgcggatg ctgcccagca
     2701 gcaacagtac ttcaatgtcc tgccaggaag cccagcgcca aaccaacttc ttaatccgct
     2761 gcaaatattt cagggaactg tttgctcgcc ggccagcggc aatcctattt gtgcacctct
     2821 gggagccggc ttcacgactc caggtgtagc aacaactgcc caatacctgc cgggtcgagt
     2881 tcgtttcaac gatcaaacat ttgttggctt tggcccgatc ttccccttca ccctggccgt
     2941 cgacaagaat ttcgagtacg cctccgccaa ccagggcaac tttacgattg agcgccaact
     3001 gacaagcaac atgtctttct cgggaagtta tatctttgtc ggcgcgcacc atttgccgca
     3061 tccgcaagac atcaatgcgc cgcagcagaa tctgttgatt gaaaacttcc ggcgcttcgc
     3121 aggtcgtaat ccggccagca atagcgaagc ctatttgttc tcgataccga atgcctgtcc
     3181 cggtgccggt tgtccgccag gatttaccgt aatcattccc ggacttgtcg ggcgcaacgg
     3241 tttgggtcag ggcgtagtaa atccaattgt tgccaacttc ttccgaccca atgcccctaa
     3301 ctacttcttc gctaactcga tcaccggtgg tttggtgacc aaggccgtgc tcgacgccgc
     3361 tctggccgcg tctaacagtc tgcgcacgcc gggctcgctc actcctttcg gtgaaattga
     3421 cgcgcagtta tcagacggaa attccactta ccacgcgatg aacctggaag tgaagcggcg
     3481 cttctcaaac aacttccagt ttctcgcttc ttacacctgg tcacattcga ttgatgattc
     3541 cagcgactta caaacattac tcaagccgca ggacaatcga aacttccgcg ctgaacgcgc
     3601 tgactcgctc ttcgaccagc gtcatcgctt cgtcttcagc ggtgccctaa cctctcccgc
     3661 gtcctggcgc ggttcgtcca gcgcggcgca tcgcttcctg gcagacttca cgattgcccc
     3721 tattttcgaa atgtcttccg gaagaccgtt caacattttg acaggcgtgg attctaacgg
     3781 cgacttgcaa ggttcgaccg atcgcccggc cgtcgcggcg gatggaacgc tgttcatccc
     3841 caccacggcg tttactaccg gcagtctcgg acgcaacctc ggcatcactc ataggtatgc
     3901 ttcgcttgat ctgcgtgtga tgaaagcaat tcctttaggc gagcgcgtgc gactggacct
     3961 gattgccgaa ggcttcaacc tcttcaatcg attcaatgag gcagcagcgt cgccactctt
     4021 cagcgacgtc aacgctttcc gtcagcgggc aggcaatggt cgctacttca gcaacccgac
     4081 tgcttcgtat gatccgcgcc agtttcagtt tggcctgaag ctgaacttct aagaaccttc
     4141 agtcccagcc ttttgatttg ggtacttcaa aagcgatcgg cgcaagttgc cggtcgcttt
     4201 tgtttttgta ggactgaaac tggcgcccga tttcaattgt cgctacgcga cgaagacagg
     4261 tggacgattg atcccggcgc ttccacccc
//
LOCUS       DASUEX010000335         3428 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1100410, whole genome shotgun sequence.
ACCESSION   DASUEX010000335 DASUEX010000000
VERSION     DASUEX010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3428
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1100410"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1055
                     /locus_tag="VFV61_05625"
     CDS             <1..1055
                     /locus_tag="VFV61_05625"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX4899991.1"
                     /translation="QLNTRIGIASITKYMTSVILNRLIESNKVALADKLNKYIPDFPN
                     GDKITIEMLARHRSGIPHRVMPSEAESLAYTSAEFIEKVKQAKLAFEPGTARLYSSAG
                     YSVLARVLEIVSGKTYAQLLQEFVFTPAGMTDSLDFEGDAVIERRAQDYYLSPNGLVN
                     VPLKNYSFLVGAGSVFGTAHDVYRFGEAVLDGKYGEGLKASLLGETTFSGSGSTNGHR
                     SYFEFARDKKYGYVVLANSAGAFDLISQGVLQILQGKEPLVRSFTLPKIVGNPNKDLA
                     EFTGHYKRSDGGETDIVIRNGFLYSGDIKLYPTRPDCFFEYRFFGEVCFLRAESAKIK
                     EIKWKGHNFDFTWVKE"
     gene            complement(1218..1511)
                     /locus_tag="VFV61_05630"
     CDS             complement(1218..1511)
                     /locus_tag="VFV61_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004717067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEX4899992.1"
                     /translation="MFALIGWIIFGLIAGVIAKAIMPGKDPGGILITIILGIVGAIIG
                     GFVGKALFGYGRTVNDTGDLSQPGFLMSLVLAVVGAIVLLAVYRLIASRRTSV"
     gene            2141..>3428
                     /locus_tag="VFV61_05635"
     CDS             2141..>3428
                     /locus_tag="VFV61_05635"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX4899993.1"
                     /translation="MSVLKYRALTPDIEFWMGMIPCQAACPVKTDAGKYVQLIAEGQF
                     KDSYLVARSPNPFASVCGRVCAAPCEDACRRGKIDAPITIRALKRFVTEKYGVESMEP
                     DTQDGLLQGIRDAGNKRRGHLPVLQESRAKVARGQKVAVIGAGPAGIAAAHDLALMGY
                     RVTIFEATNQAGGMMFHGIPEFRLSRSIIEKEIQQVVQLGVEIKLNTPLTDKFGIREL
                     KAEGYESVFLSVGVQKGRDMNIEGSNLDGIIKAIDYLLNINNGYRVNLGRKVLVIGGG
                     FVAFDAARIALRAGLETAGTESGAGGDEQGGQGLHAAIDAARAAIRAGAVEVNIASLE
                     SFAEMPVMKTTQGREEFEEARKEGVRFIPQRGAKRFIGQNGRLKAVEFTGVKRTYDED
                     GRFNPVYDPEFSEIFDVDSVILAIGQQADLSFLTADD"
BASE COUNT          841 a          889 c          929 g          769 t
ORIGIN      
        1 accagctcaa tacgagaatc ggaattgctt cgatcacgaa gtacatgacg agcgtgatct
       61 taaatcgtct tattgagagt aataaggtcg ctcttgctga caagctcaac aagtacattc
      121 cagattttcc aaatggcgac aagattacga tcgagatgct ggcgcgccac cggtccggca
      181 ttccgcaccg cgtgatgccg tccgaggcag agtctctggc ttacacttcg gcggagttca
      241 tcgaaaaagt gaaacaggcg aagttagcat tcgaacctgg gacggctcgc ctctacagtt
      301 ccgcgggcta ttcggttctg gcacgcgtat tggagatcgt ttccggaaag acctacgcac
      361 agctcttaca ggaatttgtg ttcacaccag cgggaatgac agactcgctc gattttgaag
      421 gcgacgcagt catcgagcgt cgggcgcaag actattatct ttcgccgaac ggcctcgtga
      481 atgttccact caagaattat tcgtttctgg tcggggcggg ctcagtcttt ggcactgccc
      541 atgatgttta cagattcggc gaggcagtgc tggatggaaa atatggagaa ggcttgaagg
      601 catcactcct gggggagacg acatttagcg gcagcggaag caccaacggg catcgctcgt
      661 atttcgagtt cgcgcgcgac aaaaagtatg gctatgtggt actggcaaat tccgccggcg
      721 cttttgatct catatctcag ggggtattac agatcctgca aggaaaagaa cctttggtaa
      781 ggagctttac cttgccgaag attgttggca atccgaataa ggacctcgcc gaattcacgg
      841 ggcactacaa acgcagcgat ggcggtgaaa cagacatcgt gatcagaaac ggatttctgt
      901 attcaggaga catcaagctt tacccaacta gaccggattg tttttttgaa tacagattct
      961 ttggcgaggt ctgttttctc cgcgctgaat cggcgaagat caaagagata aaatggaaag
     1021 gccacaactt cgactttacc tgggttaaag aatgaaaccg gcgctgtggc ggacgcagag
     1081 tcgctgccca gcgttccaga caagctcacc ttgtgcacgc acgtgcacgg aacaaaactc
     1141 ttgtttgaaa gagagcgttt gaagcgctcg cgagactacc tcaggccttc aaatccccgg
     1201 ccctcggcta ctcccagcta aacggaagta cgacgactcg cgatcagccg atacacggca
     1261 agcaagacga ttgcgcccac aaccgccaga accaggctca tcaagaatcc tggttggctt
     1321 aggtcgccgg tatcattcac ggtccgaccg tagccaaaga gcgccttccc gacaaagcca
     1381 ccaataattg caccgacgat gccgaggatg attgtaatca ggatcccacc cgggtccttg
     1441 ccgggcataa ttgccttggc gatgactccg gcaatcaagc cgaaaatgat ccacccaatc
     1501 aaagcaaaca tttttgtctc cttttgagaa gaatggtctc aacacatcta agggttggtg
     1561 cgcgctttcg aagttagttt agacctttat tcagtagaag caatagcaac cagccaacac
     1621 attgctggtt gccgattgcg gatttccgat tggctctttc gctacggtct acttggcaat
     1681 cggcaatcgc aaatcgacaa tcggcaactc actttgccgc ttcggatgat tcgtctcatg
     1741 cgggtgtgct ctacatcaca atacgtgcca cctctcgtct gactgaacca ctcaacttcg
     1801 ttgcctgttt tcaccttcaa atgtgagcac tcagtttcaa agttcgcaat ttttccgggc
     1861 attattaaag ggattggctg ctcaaaactt cgcactctct gcggaagttt tcccagtgtc
     1921 agttccaacc acgttcaaga ggcccgcctt agaaccagca aaaatctgcg ttctggaaag
     1981 gttgtgagct cgtaagtcgg ttaacggctc ctacctaagt cagcagttct tgccattcga
     2041 caccacggaa cctgatctgg atattccttg tagaactcgg ttttcgcttt gggagcagct
     2101 tttgcttgac gtaaagcgat cagcggaagc ggagttcagt atgagtgtac tcaaatacag
     2161 ggcgctcact cccgatattg agttctggat gggcatgatc ccttgccaag ccgcttgtcc
     2221 ggtgaaaacg gacgccggca aatacgttca actcattgcc gaaggtcagt tcaaagactc
     2281 ctacctggta gcccgctcgc ccaatccctt cgcttctgta tgtggacgtg tgtgtgccgc
     2341 tccgtgcgag gacgcatgcc ggcggggaaa gatcgacgcc ccgattacga ttcgcgcgct
     2401 caagcgtttc gtcactgaaa agtacggtgt cgagtcgatg gagccggaca ctcaggacgg
     2461 actgcttcaa ggaatccggg atgcaggaaa taaacggcgc ggccatcttc cggtgctcca
     2521 agagagtcgc gccaaggttg cccgcgggca aaaggtcgca gttatcggcg cgggcccggc
     2581 gggcatagca gcggcacatg acttagcttt gatgggctat cgagtcacga tctttgaagc
     2641 aacgaaccaa gccgggggaa tgatgtttca cggtattccg gagtttcgtc tctcccggtc
     2701 gatcatcgag aaggagatcc aacaagtcgt tcaactgggc gtagagatca agctcaatac
     2761 tccgcttacc gacaagttcg gaatccgcga gctgaaggcc gaaggttacg aatcagtctt
     2821 tctcagtgtc ggggtgcaga aaggccgaga catgaacatc gaaggttcaa atctcgacgg
     2881 catcatcaag gcaattgact acctgctcaa tatcaacaac ggctatcgcg tgaacttagg
     2941 acgaaaagtc ctggtgatcg gcggcgggtt tgtagcattc gacgccgcgc gcatcgctct
     3001 gcgggccggg ttagaaactg caggaacaga gagcggtgcc ggcggtgatg aacaaggagg
     3061 ccagggacta catgcggcga tagacgccgc acgcgcggcg atccgggcgg gcgctgttga
     3121 ggtcaacatc gccagcctcg agtcgttcgc agagatgccg gtgatgaaaa cgacccaggg
     3181 gcgcgaggag tttgaagaag cgaggaaaga gggtgtgcgc ttcatcccgc aacgtggagc
     3241 taaaagattc atcgggcaga atggccggct caaggcggtc gagtttaccg gcgtcaaacg
     3301 aacctacgat gaagatggcc gtttcaatcc tgtctacgat ccggaattca gcgaaatctt
     3361 cgatgttgat tcggtgatcc tcgccatcgg acaacaggcg gacctttctt ttttgactgc
     3421 cgacgacc
//
LOCUS       DASUEX010000336         3701 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1100520, whole genome shotgun sequence.
ACCESSION   DASUEX010000336 DASUEX010000000
VERSION     DASUEX010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3701
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1100520"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1046)
                     /locus_tag="VFV61_05640"
     CDS             complement(<1..1046)
                     /locus_tag="VFV61_05640"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX4899994.1"
                     /translation="MRKLSSLILCLAFTATACARSSAPLSVAAAAVTESTEQSTRDQS
                     VAVALQGFTTVEGADLAARLEAASQRARSKSGPYWSAYSFDVRPNVAIDPGVREFHGS
                     MNTVGDTSVFVGTTAGGMTVETRNLAVFLLRDSGSNQITRMEVYNLERKREYSGYPVY
                     WLGRANNEESLNYLRALSAAAPQSLLSERAVLGISLHDDQRVASLLKTFISTSQNQRI
                     RAASVYWLGQVGGEQAFLADLVRNTGEEVKLRRQAAHAIGESRDRGALNTLQSLYDAV
                     KEIEVRRGIIQAAGNNQEQEAAFAFLLKIAKSDPEKQSRRTAVQQLGEFERANIVEEL
                     SKLYSGETDADVKKA"
     gene            complement(1177..2709)
                     /locus_tag="VFV61_05645"
     CDS             complement(1177..2709)
                     /locus_tag="VFV61_05645"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX4899995.1"
                     /translation="MTAILISLKSLSRICRSPFGLAVLIVLSLSIAGVVRTRQAFGHD
                     QDVQKLNRFVQTPRGNPAAMKTFREGRDLIEGEKWQQAAEKFDGFINEFPKDRDVDAA
                     LYWYAYALQKQGKKDEAAAPLLRLIKNFPSSSWRREAEAMLVVMGRREAVQQALDQNN
                     CEIKILALQSLFQANEDRAIAFVSEVLQSNAEATCPGIKAAAVSLLGSHGGARATPIL
                     LNLARQQGDLKLRLTAIRRLGEQNGDAIADELVKLYEADRTLEIRTQILRAFAEMHSA
                     KSQAKLMEIARSSDDLNLRQVAVRYLGEQEDAASLDELIRLFDSDRTPEIRSQVLRAL
                     AEREDPKAHAKLLEIARKGETPELRIEAIRRIGDRGRGSLDDLLQLFNAETNQQIKQG
                     LIRAYAEMDDPRARAKLFEIARAADSLDLRMYAIRRLGDKNDEQTVEQLISLYDSETD
                     VQVKTVLIRSFGDSSQKSAVRKLISIARDSQSVDLRKLAVRMLGESKDPDALKFLEDL
                     LK"
     gene            complement(2857..>3701)
                     /locus_tag="VFV61_05650"
     CDS             complement(2857..>3701)
                     /locus_tag="VFV61_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899996.1"
                     /translation="TYTSTVDAAAIDSALAKVLPSRAAETAPTASRSWRERFAAFGLA
                     DAFSPRFAATAALIVLAVAIGIVALLLKQPTSDLAINDAPPIDTSGAEKVVVEAQKPQ
                     AQKPGSTAQPAASSDKAEKPKPAQTPAQNVPRAPRRRREIAPNYSAIEDNVATNTQGL
                     DRAEVELMTARHLEQSELLLRAFRNVRTVKRGEVPEIRYERQRAQKLLYQNMLVRREA
                     DAKGDVQVSALLGSLEPILLDIANLRDKPRNEEVVAIKDRVERKSLVPLLQINSAAVA
                     RAYE"
BASE COUNT          727 a         1062 c         1068 g          844 t
ORIGIN      
        1 gctttcttca catccgcgtc agtttctccg gagtaaagct tagatagttc ctcaacgata
       61 ttcgcccgct cgaactcgcc gagctgttgc acagccgtgc ggcgtgattg tttttccgga
      121 tcgcttttgg cgatcttgag gagaaacgcg aacgcggctt cctgctcctg attgttgcct
      181 gccgcctgga tgatgccgcg tcgcacttcg atctctttga cggcgtcgta gagactttgc
      241 aaggtattga gcgcaccgcg atcccggctc tcgccaattg cgtgagcggc ctgtcgccgc
      301 aacttaacct cttctcccgt gttacggacc aggtcggcga ggaatgcctg ttcaccacca
      361 acctgaccga gccaataaac tgatgccgca cgaatgcgct ggttttgtga ggtgctaata
      421 aaagttttca gcagcgacgc tacacgctga tcgtcgtgca acgagatgcc gagcacggcg
      481 cgttcgctca gcagactctg cggcgcggcg gcagacagcg cgcgtaggta gttcaagctc
      541 tcttcattgt tggcgcgacc gagccagtag acgggataac cgctgtactc gcgtttacgt
      601 tcaaggttgt aaacctccat gcgcgtaatt tggttgctgc cggagtcgcg caacaagaac
      661 acagcgagat ttcgggtttc gacagtcatg ccacctgccg tcgtgcccac gaacaccgaa
      721 gtgtcgccaa ctgtattcat cgaaccatga aactcgcgca cgcctgggtc gattgcgaca
      781 ttgggacgca catcgaatga gtaagccgac caatagggac cagacttgga ccgggcccgt
      841 tggctggccg cttccagtcg ggcggcgaga tctgcgcctt ccacggtggt aaaaccctgg
      901 agcgccactg cgaccgattg gtcacgcgta gattgctctg tcgattcggt taccgctgca
      961 gcggcgacgc tgagcggagc gctgctgcga gcgcaagcag tggcggtgaa agccaggcac
     1021 aaaataagtg atgagagttt tcgcatgatg actttccttt ccaaaacttc aggtattcgg
     1081 tatcgggtat cggatgtcgg gtaacaggtg ccaggtatcg ggtagcgccg gtgttagttt
     1141 cttaaacctg aaacccgtcc cccgacacct gtttccctat ttcaacaggt cttcaaggaa
     1201 tttcagtgcg tcggggtctt tgctttcgcc gagcatacgc acagcgagct ttcgcaaatc
     1261 caccgactgc gaatcgcgcg caatcgaaat caacttgcga actgcgctct tctgcgacga
     1321 atcgccaaag gagcgaatca gcactgtctt tacctgtacg tccgtttcgg agtcgtaaag
     1381 gctaatcagt tgctcgactg tctgctcgtc gtttttgtct cccagacggc ggatggcata
     1441 catacgcaag tccagcgagt cggcagcgcg ggcgatctca aagagcttgg cgcgggcgcg
     1501 cggatcgtcc atctctgcgt aggcccgaat caggccctgt ttgatctgct ggtttgtttc
     1561 tgcgttgaag agttgcagta aatcgtcaag ggaaccgcgg ccgcggtctc ctatgcggcg
     1621 aatggcctcg atgcgtaact cgggagtttc gcctttccgg gcaatctcga gcagtttggc
     1681 gtgggccttc gggtcctcgc gctccgccag tgcgcgaaga acctggctgc gaatctccgg
     1741 ggtgcgatcg gaatcgaaga gacgaatcaa ctcgtcaagc gaggcggcat cttcctgctc
     1801 gccaagataa cgtacggcca cctggcgcaa attcaaatca tcgctactac gcgcaatctc
     1861 catcagcttc gcttgcgact tggcgctgtg catttcggcg aaggcgcgca ggatctgcgt
     1921 gcgaatctcg agggtgcgat ctgcctcgta gagtttcaca agctcatcag cgatcgcgtc
     1981 accgttctgt tcacctaaac gccggattgc cgtcaggcgc aatttcaaat cgccttgttg
     2041 gcgcgcgaga tttagcagaa tcggcgtggc gcgggccccg ccgtgagacc cgagcaggga
     2101 aacggccgcg gccttaatgc ccgggcaggt tgcttccgcg ttcgactgca atacttcgct
     2161 gacaaaggcg attgcccgat cctcgtttgc ctggaagagg ctctgcagcg caaggatctt
     2221 tatctcgcaa ttattctgat cgagtgcctg ctgcacggcc tcgcgcctcc ccatcaccac
     2281 cagcatggct tccgcttccc gccgccaact ggagctggga aaattcttaa tcaggcgtag
     2341 taggggggcc gccgcttcat ccttcttacc ctgcttttgc aaggcatagg cgtaccagta
     2401 aagcgcggcg tcaacgtcgc gatccttggg aaactcgttg atgaagccgt caaacttttc
     2461 cgccgcctgt tgccacttct cgccctcgat cagatcgcgt ccttcacgaa aggtcttcat
     2521 cgctgccgga ttaccgcggg gcgtttgcac aaagcgattc agcttctgca catcctggtc
     2581 gtgaccaaaa gcctggcgcg tccggacgac gccagcgatg gaaagcgata ggactatcaa
     2641 taccgcaaga ccgaagggcg aacggcagat gcggctcagg gatttcagtg aaattaagat
     2701 tgctgtcatg gttgttcctc aattaagcga ttaatcggtg atgcagattc accacacagg
     2761 gacagagaac acgagagcag cgcaggatcg agcggtgaca agattcgctg ggccatttcc
     2821 gtgttccctg tgcctctgtg gtggaactgc gtgacattat tcataagcgc gtgcgaccgc
     2881 ggccgaattt atttgtaaga gcggaactaa gcttttgcgc tcaacacggt ccttaattgc
     2941 gactacctct tcattccgtg gcttgtcgcg caggttcgcg atgtcgagaa ggatcggctc
     3001 aaggctgccg agcagggcag acacttgcac gtctcccttt gcgtccgctt cgcggcgtac
     3061 cagcatattt tggtagagca gtttttgcgc gcgctgtcgc tcgtagcgaa tctccggcac
     3121 ctcgcctcgc tttacggttc gcacattgcg aaacgcccgc agtagtaatt cagactgctc
     3181 aaggtgacgt gccgtcatca gctcgacctc ggcccgatcc aggccctgag tattggtcgc
     3241 gacgttgtct tcgatggccg agtagttcgg cgctatctct ctccggcgcc ggggcgcgcg
     3301 cgggacgttt tgcgccggtg tttgtgccgg ctttggtttt tcagccttgt cggaagatgc
     3361 ggcaggttgc gctgtgctcc cgggtttctg agcttgcggc ttttgagcct cgacaaccac
     3421 tttctctgca cctgaggtgt caattggcgg cgcgtcattg atggccagat cagatgtcgg
     3481 ttgcttcagc aggagcgcca caatgccaat cgcaactgcc aaaacaatca aggctgcggt
     3541 cgcggcgaac cgcggactga aagcgtcagc gagcccgaac gcagcgaacc gttcgcgcca
     3601 actacgagaa gcagttggcg ctgtctcggc agcacgtgat ggcaggacct tggcgagtgc
     3661 ggaatcaatg gccgccgcgt cgacggtgct ggtgtaggta t
//
LOCUS       DASUEX010000337         3000 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1306877, whole genome shotgun sequence.
ACCESSION   DASUEX010000337 DASUEX010000000
VERSION     DASUEX010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3000
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1306877"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /locus_tag="VFV61_05655"
     CDS             complement(<1..497)
                     /locus_tag="VFV61_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4899997.1"
                     /translation="MITGFNTDIEHDGVVYHVQTEDKGLETPLILSLVYSGGAILASK
                     RARYEDLIASGFSDEALSERLTRQHKLICAAIHAGRIEDLKRMSAKPIETALPDDVQL
                     REPQPEPRPEPEAELEAEPVQETESGLEILRGSEEFEALEAAQVEYNLQRERQAALEA
                     EGRTEP"
     gene            769..1242
                     /gene="ndhC"
                     /locus_tag="VFV61_05660"
     CDS             769..1242
                     /gene="ndhC"
                     /locus_tag="VFV61_05660"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEX4899998.1"
                     /translation="MPFRYYDYLPILLIFIVAAGFAAGNIFISQFIGQRKRTRTKMMP
                     YECGKDPVGDARQRFSVKFYLIAMLFILFDIEVIFLVPWAVVFKTLSAPAFGLANLIY
                     FEMIIFIALLLVGYVYVIKKGAFDWNERARLEAEAEARLLSDVEQAHIRRRKTAA"
     gene            1256..1885
                     /locus_tag="VFV61_05665"
     CDS             1256..1885
                     /locus_tag="VFV61_05665"
                     /inference="COORDINATES: protein motif:HMM:NF005012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HEX4899999.1"
                     /translation="MGLENVISAALPEVLTTRLDSLINWARKSSLWPATFGLACCAIE
                     MMNATSSRNDLARFGSEVFRASPRQADVMIVSGRVSRKMAPVLRRIYDQMPEPKWVIS
                     MGACATSGGVFDNYAIVQGVDKIVPVDVYIPGCPPRPEMLIHAVMMLQEKVMKESSRD
                     RRDVGDEEARAAFVPGTLPVTDGSTARQNVLPESRPYTIASKHERRRSS"
     gene            1908..2453
                     /locus_tag="VFV61_05670"
     CDS             1908..2453
                     /locus_tag="VFV61_05670"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEX4900000.1"
                     /translation="MPTDSNSELPLAEPAPPSSIQKTTPSQLVAALQSEHPDWITQIT
                     EALGEVTALVPREQIVGVCTYLKTAPDARFDFLADLCGVDRGIEEEPRFEVNYHLFST
                     TIFHRLRLKVVVNEDDARVPTVTVVWRTANWHERETFDLLGVVFEGHPDLRRILLPDD
                     WQGHALRKDFPLRGYEPYSLK"
     gene            2536..>3000
                     /locus_tag="VFV61_05675"
     CDS             2536..>3000
                     /locus_tag="VFV61_05675"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HEX4900001.1"
                     /translation="MSSTINLPPQFDVVDRPLDSQMTISMGPQHPSTHGVLRLELVID
                     GEMIVKATPDIGYLHTGMEKLFEYKKYQQGIVITDRMDYLNPLGNNLAYVMAVEKLLQ
                     IEIPERAQTIRVLMCELQRIASHLVWLGTHALDIGAMTVFFYCFREREKILNL"
BASE COUNT          635 a          813 c          800 g          752 t
ORIGIN      
        1 ggttctgtcc gaccttcagc ttccagcgcc gcctgtcttt ctctttgcag gttgtactcg
       61 acttgcgctg cttcgagggc ttcgaactct tcgctgcctc gcagtatctc caaccccgat
      121 tccgtctcct ggaccggctc cgcctccagc tcggcctccg gctcgggccg cggctcgggc
      181 tgcggctctc gaagttggac atcgtcaggc agcgcagttt caattggctt ggcggacatt
      241 cgcttgagat cttcaatacg gcccgcatga atcgcagcgc aaatcaactt gtgctggcgc
      301 gtcaacctct cgcttagggc ttcatcactg aaaccagaag caatcagatc ttcataacgc
      361 gcccgcttac tcgccaggat cgcgcctccc gaataaacga gcgacagaat caggggggtc
      421 tccaggccct tgtcttccgt ttgcacgtga tagacaaccc cgtcgtgctc aatgtccgta
      481 ttgaatcctg tgatcacagt aactaatgga gaatcaaagt ggagattcgc gccgcgaatt
      541 cacgcctgcg cgcgaagttg cagcgacgtt aacatatgct tttttgcgcg gtcaattatc
      601 atttccatta caattttccg cgagataacg ttgcttgaca ctctcgatcc acttcgacta
      661 cgattcggct tgtacaatga ttctcaatct ggacgccagc atgtcagtcg ccgcgacaac
      721 ggcttcagac atggagcggt ctggctgatc cggatgcggc tgagctaaat gccctttcgc
      781 tattacgact acctgccaat cctcctgatc tttatcgtgg ccgcaggttt cgctgccggc
      841 aacattttca tctctcaatt tatcggtcag cgcaaacgaa cgcgcaccaa gatgatgccc
      901 tatgagtgtg gcaaggatcc ggtgggcgac gcgcgccaac gcttctcggt gaagttttac
      961 ctgattgcga tgctcttcat tctgtttgat attgaagtca tctttctcgt tccctgggca
     1021 gttgttttca aaacgctttc tgctccggcg tttggactcg cgaacctgat ctactttgaa
     1081 atgattatct ttatcgcgtt gctcctggtt ggttatgtgt atgtaatcaa aaagggagca
     1141 tttgattgga atgagcgcgc ccggctcgaa gccgaagccg aagcacggtt actttccgat
     1201 gtagagcagg cgcatatccg caggcgcaaa acggcggcgt gattctggag ttttcatggg
     1261 tctcgaaaat gtaatttccg cagccctccc cgaagttctg actacgcgtc tggattcctt
     1321 aataaactgg gcgcgcaaat cttcactttg gccggcgaca tttggcttgg cttgttgcgc
     1381 catcgagatg atgaacgcga cttcttcgcg taacgatctg gcacgattcg gttctgaagt
     1441 atttcgcgcc agtccacgcc aggcggacgt catgattgtg tctggtcgcg tgtcgcgcaa
     1501 gatggctccg gtgctgcgcc gcatctacga tcaaatgccc gaaccaaagt gggtgatctc
     1561 tatgggagcg tgcgctacat cgggaggagt gttcgacaat tacgcaattg ttcagggcgt
     1621 cgacaagatt gtcccggtgg atgtctacat tcccggatgc ccaccgcgac cagaaatgct
     1681 gattcatgcg gtgatgatgc tacaggaaaa agtcatgaag gagtcgtcgc gcgatcggcg
     1741 cgatgtggga gacgaggaag cacgcgccgc gtttgttccc gggacacttc ctgtgactga
     1801 tggctcaacg gcgcgccaga atgtattacc cgaatcgcgc ccctacacga ttgcctctaa
     1861 acatgagcga cgtcgctcgt cgtgatgcgg cggatgtcta aagcttaatg ccgactgatt
     1921 caaatagtga gctgccactg gcagaaccgg cccccccaag ttcaatccag aaaacgacgc
     1981 cgtcccagtt agtggcggcg ttacagtctg agcatccgga ttggatcacg caaatcacgg
     2041 aggcgctcgg cgaagttact gcgcttgtgc ctcgtgagca gatagtcggt gtgtgcactt
     2101 acttgaagac cgcacctgac gcgcgttttg atttccttgc agacctttgc ggtgtcgatc
     2161 ggggaatcga ggaagaacca cggtttgaag ttaactatca cctcttttcc acgacgatat
     2221 ttcaccggct ccggctgaag gtggtggtta acgaagatga tgcccgggtc ccgaccgtta
     2281 ccgtagtttg gcgaacggca aactggcatg aacgggagac tttcgatctg ctgggcgttg
     2341 ttttcgaagg acatccggac ctgcggcgca ttctcttgcc tgacgactgg cagggtcatg
     2401 cattgcgcaa agattttccg ttacgaggat atgaacctta ttcgctgaag tagtcctgcg
     2461 tccaaagtcc aaagtcccaa gtcaatacag actgcgcggg ctcggacttt ggactttgga
     2521 cattggacat tggacatgag ttccaccatt aatctgcctc cccaattcga cgtcgtcgat
     2581 cgaccgctcg attcccagat gacgatatcg atgggaccac agcatccttc gacgcacggc
     2641 gtgcttcgtc tggagctggt gattgatggc gagatgattg tcaaagcgac gccagacatt
     2701 ggctatctcc atactggcat ggagaagctc tttgaataca agaagtatca gcaggggatc
     2761 gtcatcaccg atcgcatgga ttacttgaat cccctcggca acaatctcgc ttacgtaatg
     2821 gcggttgaga agctgctgca gatcgaaatc cctgaacgcg cacagacgat tcgggtcctg
     2881 atgtgtgagt tgcaacggat tgcttcccat ctcgtgtggc tgggtactca tgcactcgat
     2941 atcggcgcga tgacggtctt cttttattgc ttccgcgagc gcgagaaaat tctcaacttg
//
LOCUS       DASUEX010000338         2397 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_634241, whole genome shotgun sequence.
ACCESSION   DASUEX010000338 DASUEX010000000
VERSION     DASUEX010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_634241"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1414
                     /locus_tag="VFV61_05680"
     CDS             <1..1414
                     /locus_tag="VFV61_05680"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HEX4900002.1"
                     /translation="INSITVHPTRPEMVYIGTNNYGVMVSYDSGKNFVPTNGGYSGRF
                     ANTIVADREMPSRIYAATINTATGGGFFFVSSDNGDTWRPSMRNMPSRLITYSILQDQ
                     QDPNTIYLGTNLGVYRSTDRGASWAPIWAANGPPADAKKKRTSVRGKKKAVAQIQRRA
                     ASVSKVDEVIRQAQESLNASGYNVGAPDGRAGSQTLLMLKRYQTDRHLPATGKFDELT
                     LAALGISRDQTPSENGEAASFLILTDAVNALVQVPDPQTGRLGFLAATNAGLYKSSDP
                     AKGWRKLPFGPGLDPRATCVSVSVSQPDTIWVGTADSGALVSRDGGNSWSQATGLPTD
                     APVNTIAQDAQRPNYVYVGTKQAFYSSHDGGNSWSRRGGNLPFGDFTSILINPRNGDE
                     IFVGNAFQNGEFGGGVYRTVDAGITWARIDPKEHRLPSQRIWSLAFDSQAQNTLFVGS
                     HSAGVYVVPRRTEALSTASQ"
     gene            1792..>2397
                     /locus_tag="VFV61_05685"
     CDS             1792..>2397
                     /locus_tag="VFV61_05685"
                     /inference="COORDINATES: protein motif:HMM:NF012784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEX4900003.1"
                     /translation="MFSEEPKLSKSEADESISSGEQQPSPQPAPASQTQQQTQPIAAH
                     DEDEPRQGGDVDFGQLLDQFEQEQATLQEGEVVRGTVVGISERGVVIDFGYKSEGIVN
                     PAEFSEDGVLTVKPGDEVEVLVKSMETADGLPVLSRADAVRMKAWDDLEKAYRDGTNV
                     TGRVIERIKGGLRVDIDGIAAFLPGSQVDVRPVRNLDSLRNQ"
BASE COUNT          575 a          625 c          665 g          532 t
ORIGIN      
        1 gatcaattcg atcacggtcc atcccactcg ccccgagatg gtttacatcg gaaccaacaa
       61 ctacggagta atggtgtcct atgatagtgg taagaatttc gtgccgacca atggaggcta
      121 cagcggaagg tttgcgaata cgatcgttgc cgatcgcgag atgccaagcc gcatttatgc
      181 cgctaccatc aacactgcga cagggggagg gttcttcttt gttagtagcg ataacggcga
      241 cacgtggcgg ccttcgatgc gcaacatgcc atcgcgattg attacatact ctatccttca
      301 ggaccaacag gatccaaaca ctatctacct tggtactaac cttggtgtct atcgctctac
      361 cgatcgaggt gcatcttggg ctcctatctg ggcggccaat ggaccacccg ctgacgctaa
      421 gaagaagcgc acttcagtac gcggcaagaa gaaagcggtt gcgcagattc aacgccgagc
      481 agcgtccgtc agcaaagtcg atgaagtgat tcggcaagcg caggagtcac ttaacgcgag
      541 tggttacaat gtcggcgcgc cggacgggag ggctggtagt cagacgctac taatgctcaa
      601 gcgctatcaa acggacaggc atcttcctgc aactggcaag tttgatgagc ttacgcttgc
      661 cgcacttgga atttctcgag atcaaactcc ttcagagaat ggcgaggcgg cctcatttct
      721 gattctcacc gatgccgtaa atgctttggt ccaggtgcct gatccgcaga caggcaggct
      781 tgggtttcta gcagcaacca atgccggcct ttacaagagt tcagaccctg caaaaggttg
      841 gaggaaattg ccatttggtc caggcttaga tccaagggca acctgcgtat cagtaagtgt
      901 cagtcagccg gacactatct gggtgggcac tgcagattcg ggcgcactgg tttcacgcga
      961 tggcggcaat tcatggagcc aggcaacggg tttacccacg gatgccccgg taaacaccat
     1021 cgcacaggac gctcagcgac ctaattatgt ctacgttgga actaaacagg ctttctattc
     1081 gtctcacgat ggcggtaaca gctggagtcg tcgtggtggg aatttaccgt ttggtgactt
     1141 caccagtatc ttgatcaatc cacgtaatgg cgacgagatc tttgtgggca atgccttcca
     1201 gaacggcgag tttggcggtg gcgtatatcg cactgtcgat gccgggataa cgtgggctcg
     1261 aattgatccc aaggagcacc gtttgccgag ccaaagaatc tggtctctcg cctttgactc
     1321 acaagctcaa aataccctct ttgtgggctc tcactccgct ggcgtctacg tggtgccgag
     1381 gagaactgag gcgctctcga cggcgagtca ataacactgt tgcgcttctc aaaattagtt
     1441 ctgaagtaac gtaataaagt ctgacacgat caaggtttag cgccttgaca cacgcttgtt
     1501 gcgacgtgta cattgcccac ctacccggta aagcggcagg tgattgtcgg accaagcggg
     1561 tgactacggg cacggcggaa atcgaaacct ataaaacctc cttttccaaa tcttaacggc
     1621 tcgcattcct cgctccagct tcaaattccc gaccaatcgc cagcgaagtt cagccaaacg
     1681 gccgctaata ctgtccgcga gtcgttgttg ttttgatggc cagtgtgcga aggagcgcga
     1741 aatgatcttt aacgcgctca ctagatagtt ccgggtgcca caggagtctg catgtttagt
     1801 gaagaaccca agctgagcaa gagcgaggcg gatgagagta tctcttccgg tgaacaacaa
     1861 ccctcaccgc agcctgcgcc agccagccaa acccaacaac aaacacagcc aatcgctgcc
     1921 catgatgaag atgagccgcg tcagggcggc gatgtcgatt tcggccaact actcgatcag
     1981 tttgaacaag agcaggccac ccttcaagag ggcgaggtcg ttcgcggaac cgtggtaggc
     2041 atcagtgaac gtggtgtcgt aatcgatttt ggctacaaat cggaaggcat agtgaacccg
     2101 gcagagttca gcgaggacgg cgttcttact gttaagcctg gtgatgaagt cgaagtgctc
     2161 gttaaaagca tggagactgc cgatggcctg cctgttctct ctcgcgcgga tgcagtgcga
     2221 atgaaagcgt gggacgatct ggaaaaggca tatcgtgacg ggacgaacgt tacaggtcgc
     2281 gtgattgaac gtatcaaggg tggtctgcgc gttgacatcg acggcattgc ggcgttcctg
     2341 ccgggctcgc aggtcgacgt tcggccggtt cgcaatctcg acagtctcag gaatcag
//
LOCUS       DASUEX010000339         2312 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1438156, whole genome shotgun sequence.
ACCESSION   DASUEX010000339 DASUEX010000000
VERSION     DASUEX010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1438156"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /locus_tag="VFV61_05690"
     CDS             complement(<1..585)
                     /locus_tag="VFV61_05690"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX4900004.1"
                     /translation="MSFWLDEHVTAAETDTGTTDYGLTQKAAQGDMGAFEQLYQRHNR
                     RVYSLCLRMTGNVSEAEDLAQEVFIQLFRKIGSFRGESAFTTWLHRLTVNQVLMHFRK
                     RGVKMEQTTEDGETPVQIVKGTENPNAMPVVDRIALDKAISQLPPGYRTVFTLHDIEG
                     HEHEEIARMLGCSVGTSKSQLHKARMKLRGLLRQQ"
     gene            complement(831..>2312)
                     /locus_tag="VFV61_05695"
     CDS             complement(831..>2312)
                     /locus_tag="VFV61_05695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEX4900005.1"
                     /translation="RGETDKLTVTIVGLPDAAVRESRERIRAAITNCAFFFPFEKVTI
                     NLAPADVRKEGASFDLPIALGLLGANGDLAEANNLIDVLCVGELSLDGRVRPIKGALP
                     ITILARDALIKHVILPDENAKEAAVVSGVNVYPVADLRSAVDLVAALRSANPPEPLRV
                     DPNEILHRADNYSVDFREVRGQVSAKRALEVASAGSHNILLIGPPGSGKTMLAKRLPT
                     ILPPLEFEAALELTKIHSVAGMTGRAGLVVERPFRSPHHTISDAGLIGGGAVPRPGEV
                     SLAHQGVLFLDELPEFDRSVLEVLRQPLEDQKVTISRAAMSLTFPASFMLATAMNPCP
                     CGFWNDPTRECRCTTLQIQRYVGRISGPLLDRIDIHVDVPAVKFRELAGEPASDTESS
                     AMMRDRVMQARDRQQQRLAKEKIFANAAMTPRMIRRFCRIDAESEQMLERAMIKQGLS
                     ARAYDRILKVSRTIADLEGFEKIQAPHVSEAVGYRSLDRTYWT"
BASE COUNT          494 a          671 c          629 g          518 t
ORIGIN      
        1 ctgttgcctt agcaggccac gtaacttcat gcgggcctta tgaagctggg attttgaggt
       61 accgaccgag caccccagca tccgcgcaat ttcttcgtgt tcgtgccctt caatgtcgtg
      121 aagtgtaaaa accgtacgat acccgggcgg cagttgcgaa atggctttat cgagggcaat
      181 ccggtcaaca accggcatcg cgtttgggtt ttccgtgcct ttgactattt gcaccggggt
      241 ttcgccgtcc tcggtggtct gctccatctt cacgccgcgt ttgcggaagt gcatcaggac
      301 ctggttcacc gtaagccggt gcagccaggt ggtaaaggcc gactcgccac gaaagcttcc
      361 aatttttcta aagagctgaa tgaagacctc ctgagcgaga tcttcagcct ccgaaacatt
      421 tccggtcatc cgcaggcaga gcgaataaac gcgccgatta tggcgctgat agagctgctc
      481 aaaggctccc atgtcgccct gagcggcttt ctgtgtcagg ccgtaatcgg ttgttccggt
      541 atcggtttcg gctgcggtca cgtgttcgtc tagccagaac gacaagaagt tttgtgttct
      601 acttgccctt ctttaccttt acgccaagtt atcacgcgtc cgccgaattt caaggagcgg
      661 tacagtaaac ctccgcaaaa tcccccgttt gggagttgcg aggttcggta ccctctggcg
      721 ggggtgtgat gcggcgtttt aagcgcgtcg ttgcctttaa cggctaaaag ttagccgttt
      781 tctacgatca cagcaacacg gcagcccatt ttgtcggatg agagcgacgc tcacgtccag
      841 taggttcgat ccagcgagcg gtaacccacc gcctcggaca catgtggtgc ctggattttt
      901 tcgaaaccct ccaaatccgc aatggtccga ctcactttca ggattcgatc gtaggcgcgt
      961 gctgacaggc cttgcttgat cattgctcgt tccagcatct gttcactctc cgcatcaatg
     1021 cgacagaagc ggcgaatcat gcgcggcgtc atggcggcgt tggcgaagat tttctccttg
     1081 gcgaggcgct gctgctggcg atcgcgcgct tgcattaccc ggtcccgcat catcgccgag
     1141 ctctcagtgt cgcttgccgg ctcaccagct aactcgcgaa acttcaccgc cggcacgtcg
     1201 acatgaatat cgatgcgatc gagcagcggg ccggagatgc ggccaacgta gcgttgaatc
     1261 tgtaaggtcg tgcaacggca ttcgcgcgtt gggtcattcc agaaaccaca aggacaagga
     1321 ttcatggcag tggccaacat gaaggacgcc ggaaacgtaa gcgacattgc cgcacgacta
     1381 attgttactt tctgatcttc taaaggctgg cgcagaactt ccaggacgct gcgatcgaac
     1441 tccggcaact catccaaaaa caaaacgccc tggtgcgcga gtgaaacttc gccgggacgc
     1501 gggacagcgc cgccgccaat caaacccgcg tcgctgattg tgtgatgggg cgaacgaaac
     1561 ggcctttcaa ctacgagtcc ggcgcggcct gtcattcctg ctaccgaatg aatcttcgtc
     1621 aactcaagtg ccgcttcgaa ttccaaaggc ggcaggatcg ttggcagtcg cttcgccagc
     1681 atcgtcttgc cggatcccgg agggccgatc aggagaatgt tatgtgaacc ggcactcgca
     1741 acctcaagtg cccgcttggc agaaacctgc ccccgcactt cgcgaaagtc cacgctgtaa
     1801 ttatccgctc gatgcaaaat ctcgttcggg tctacccgca gcggttccgg tggattagca
     1861 gacctgagcg ccgccaccaa atcaaccgcg ctgcgcaggt cagccactgg ataaacattg
     1921 acgccggaaa ctacagctgc ttccttcgcg ttctcgtccg gcaagatcac gtgcttaatc
     1981 aaagcgtcgc gcgccaggat cgtgattggc aacgcgcctt tgatcggtcg cacccggcca
     2041 tcgagggata actcgccgac gcataaaaca tcgatcaggt tgtttgcttc cgccagatca
     2101 ccattggcgc caagaagccc caacgcgatg ggaaggtcaa agctagcgcc ttccttgcgc
     2161 acatccgcag gagcaaggtt gatcgtgact ttttcaaacg ggaagaagaa tgcgcaattg
     2221 gtgattgcgg cacgaatacg ttcgcgcgat tcgcgcacgg cagcgtccgg taagcctacg
     2281 atggttactg ttaacttatc ggtttcacca cg
//
LOCUS       DASUEX010000340         4323 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1438210, whole genome shotgun sequence.
ACCESSION   DASUEX010000340 DASUEX010000000
VERSION     DASUEX010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4323
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1438210"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="VFV61_05700"
     CDS             <1..325
                     /locus_tag="VFV61_05700"
                     /inference="COORDINATES: protein motif:HMM:NF024639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HEX4900006.1"
                     /translation="KMKLMGLTEFFDLPGAFGDESHDRSDLPELAAARIKQHLGVDLE
                     SHQFIVIGDTPNDIACAKHFGARSLAVGTGRLYSSKDLLACDPDAFVPNLADPEQVLR
                     VLSQL"
     gene            389..868
                     /locus_tag="VFV61_05705"
     CDS             389..868
                     /locus_tag="VFV61_05705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900007.1"
                     /translation="MPNKPPADLIKFLKPYERAIQKLALDLRLIVIDELAPCHENIYD
                     AYSAVAIGYGSSDRLSDGVCHIAVYAHGVNLGFNQGALLEDPEDELLGTGKRIRHLSL
                     KSQADLTRPEIRMFLRRALALARDDAQKLGETISMGKRPRTVISTVKAIYKKKRRPA"
     gene            complement(933..1499)
                     /locus_tag="VFV61_05710"
     CDS             complement(933..1499)
                     /locus_tag="VFV61_05710"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX4900008.1"
                     /translation="MDWPTLPTITTPRLVLRWISHDDVDALFEVFSNVEVMRYWSTPP
                     LADRAAAVALVNEIHDSFERQVMLKWGVARLADNKVIGTTTLYNVDFSNRRAELGYAL
                     GRLHWGHGYMQEALQALLSYAFETLDLRRLEADVDPRNQASIQTLERLGFQKEGFLRE
                     RWEVGGEIQDALFYGLLRPEWKRNDTIS"
     gene            1648..2469
                     /locus_tag="VFV61_05715"
     CDS             1648..2469
                     /locus_tag="VFV61_05715"
                     /inference="COORDINATES: protein motif:HMM:NF042696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX4900009.1"
                     /translation="MRYREIKPQSELRRFVECFWTLESEHDDLQVSPESILPDGCVEL
                     ILNSGAPFHEVKDSGERIRQPAHFVVGQMTRPVLIAPTGAVELIGIRFQPGGTMPFLK
                     QPLHELTNRVIELEGLDSALDRELLPALNSSASLTARIQAIESCLTRRAMIFAGDRRL
                     LKLTAAAVRVNGRTTIDSLAAAAGVSARQLERRFLGEVGIGPKLFCRILRFQQVFSAV
                     VENSAGWAALAIDCGYYDQAHLIRDFQQFARQTPAVLLAHNGGLTEAFSRKHRSI"
     gene            2580..3083
                     /locus_tag="VFV61_05720"
     CDS             2580..3083
                     /locus_tag="VFV61_05720"
                     /inference="COORDINATES: protein motif:HMM:NF039391.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6265 family protein"
                     /protein_id="HEX4900010.1"
                     /translation="MRSTKLTRVLVAIALTTMFKVHLNAQNPATPAPSTLHTISELGW
                     MSGHWQTAPGGRAQIEEHWTQPAGGSMLGMSRTVIGIRTLEFEYLRLEQRADGIYYVA
                     HPKARCPGTEFKLTSVTTNGAVFENPAHDFPKRIIYRKTAAGLVANIDAGEGTKGQSF
                     SYVPMKQ"
     gene            complement(3110..3778)
                     /gene="msrA"
                     /locus_tag="VFV61_05725"
     CDS             complement(3110..3778)
                     /gene="msrA"
                     /locus_tag="VFV61_05725"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF013766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEX4900011.1"
                     /translation="MTTAILITSILFLIGFTFACSTAGASSTPLPDPEVDAPLASVKG
                     EQKAVLAGGCFWGIEAVFEHVKGVSDAKSGYAGGDAASADYRKVSSGTTGHAESVLVT
                     YDPSQITYGQLLKVFFSVAHDPTELNRQGPDRGTQYRSAIFYANEEQKRIAEAYIKQL
                     DRLKLFSRSIVTQVTPLDAFHDAEAYHQDYLAQHPNEPYIVINDMPKVENLRKQLPEL
                     FKEK"
     gene            complement(3855..>4323)
                     /locus_tag="VFV61_05730"
     CDS             complement(3855..>4323)
                     /locus_tag="VFV61_05730"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HEX4900012.1"
                     /translation="ASNTPHIVFDELQRRSSLPLISIVEAACSRVQEFGLKKIGLFGT
                     RFTMQGRFYPDVFSRNGIQLVTPNDEEQDFIHEKYMGELLNDIFLPETREQMLRIADE
                     LQKRDGIEGVILGGTELPLLLSPETFTDGQRNGIRFFDTTRIHVDRLVEELLS"
BASE COUNT          988 a         1169 c         1148 g         1018 t
ORIGIN      
        1 gaagatgaag ttgatgggac tgacggagtt cttcgacctg ccgggtgctt ttggcgacga
       61 atcgcacgat cgcagcgact tgccagaact tgccgcggcg cgcatcaagc aacatctcgg
      121 tgtcgatctc gaatcgcacc agttcattgt catcggtgac acgcccaacg acatcgcctg
      181 cgccaaacac tttggcgctc gctcgcttgc agtcggtact ggccgcctct acagctctaa
      241 agatctgctc gcctgcgatc ctgatgcgtt tgtaccgaat cttgcagatc cagaacaggt
      301 actaagggtc ttgagtcaat tgtgagtaat taattagagg tgggatgtct ccgtcaggaa
      361 gggcggccgc cccgcttcga ttaataccat gccgaacaaa cctcctgctg atctgatcaa
      421 gtttctcaaa ccgtacgagc gtgcgattca aaaactggca ttggatcttc gcctcattgt
      481 gatcgacgag ctcgcgccct gtcatgaaaa tatctatgac gcttacagcg cggtcgcaat
      541 tggttacggc tccagcgacc gccttagcga cggcgtctgt cacatcgctg tttatgcgca
      601 cggcgtcaac ctcggcttca accagggtgc gttactggaa gatcccgaag acgagctcct
      661 gggaaccggc aaaaggattc gacacctctc gctgaagagc caagccgacc tgacccggcc
      721 ggagattcgt atgtttctga ggcgcgctct cgcactcgca cgcgatgatg cgcaaaagct
      781 gggagagacc atttctatgg gtaaaagacc gaggacagtt atctctaccg tgaaagcgat
      841 ctacaaaaag aagcgtcgcc cggcataaat atcatactca tcggaacagg gtcagccacc
      901 gttccaacgg gagacattca aacgggtgct ggtcaactta ttgtatcgtt gcgcttccac
      961 tcggggcgca gcaatccata gaacaacgca tcctgaatct ctccgccaac ttcccaacgt
     1021 tctcgcagga atccttcttt ttgaaagcca agtcgctcca gagtttgaat tgaagcctgg
     1081 ttgcgaggat cgacgtcggc ttcaaggcgt cgcagatcga gagtttcgaa tgcataactc
     1141 agcaatgcct gaagggcttc ctgcatgtaa ccgtgccccc aatgcaggcg ccctaaggcg
     1201 tagcccagct cggcgcggcg attactgaaa tcaacgttat atagcgtcgt cgtgccgatg
     1261 accttgttgt ccgccagccg cgccacgccc cacttcagca tcacctgtcg ctcaaagctg
     1321 tcgtggattt catttaccag ggccactgcc gcagcgcggt ccgcaagtgg aggtgtactc
     1381 cagtagcgca tcacctcaac gtttgagaag acttcaaaca gagcatctac gtcatcgtgg
     1441 gagatccaac gcagcacgag acgaggtgtg gtgattgtcg gaagggttgg ccaatccatc
     1501 gaaggcggat tgtaacatca tcgcaattgt cgattgccga ttggcaatgg ccgatttctc
     1561 ggcgggccgg cctttcctta gcacaaagct tgaaatgttt tgtgacgggt tgttcctcgg
     1621 gtatcctttc tctcgattac ccacactatg cgttaccgcg aaatcaaacc ccaatctgag
     1681 ctgcgccgct ttgtcgagtg tttctggacg cttgaaagcg agcacgacga tctgcaagtc
     1741 tctccggagt caatccttcc ggacggctgt gttgagctga ttctcaactc cggtgctccg
     1801 tttcacgagg ttaaggacag tggtgaacga atcagacagc cggcgcattt tgtagtgggc
     1861 cagatgacgc gccctgttct tattgcgcca accggtgccg tggagttgat cggaattcgt
     1921 tttcaacccg gcggcacgat gccatttctc aagcaaccac tacacgaatt aacaaatcga
     1981 gtgatagaac tggagggact tgatagtgct cttgatcgcg aattgcttcc agcgctgaac
     2041 agctcggctt cgttaactgc gaggattcag gcgatcgaaa gctgccttac tcgccgcgcg
     2101 atgatcttcg ccggcgaccg acgcctgttg aaactcactg ctgctgccgt tcgtgtcaac
     2161 ggcaggacta ctattgatag cttagccgct gcggcgggcg tcagtgcacg ccaattggag
     2221 cgccgctttc taggtgaagt aggtatcgga cccaagttgt tctgccgcat tcttcgcttc
     2281 caacaggtgt ttagcgcggt ggtagaaaac tcagcaggct gggccgcact cgcgatcgat
     2341 tgcggctact acgatcaggc gcatctgatt agagattttc agcagtttgc tcgtcagact
     2401 ccggctgttc ttctcgctca caatggtgga ttgacggagg cgttcagccg caagcatcgc
     2461 tcgatctgat gttagttttt tgggaggtgt cgcatttttc caatacgtta gagtgaagcg
     2521 gccttagaat cctccgcgca cgtaaacatt tcctactgta cttccaacca atgagaacca
     2581 tgagatcaac caaactaacg cgggtgctgg tcgcgatcgc tctgacaact atgttcaagg
     2641 tgcatctgaa cgcccagaac cccgccactc cggccccatc tacgttgcat acaatttccg
     2701 aacttggctg gatgtccggt cactggcaga cggcgccggg tggacgcgct caaattgagg
     2761 aacactggac acaacccgcc ggaggctcga tgctcggtat gagtcgcacc gtaatcggga
     2821 ttcgaactct tgagtttgag tacttgcgat tggaacaacg agcggatggg atctattacg
     2881 ttgcgcaccc taaagcacgc tgcccgggaa ctgaattcaa actaacgagc gtgacaacca
     2941 acggagccgt gttcgaaaat cccgcgcatg attttcccaa acggatcatt tacagaaaaa
     3001 ctgcggcggg ccttgtcgcc aacatcgacg ccggcgaagg aaccaagggt caatcgttca
     3061 gctacgttcc aatgaagcag tgagtcaaaa aaccagcgga gcagtctcat tatttttctt
     3121 tgaataactc cggcagctgt ttgcgcaggt tttccacttt gggcatgtcg ttgatgacga
     3181 tgtagggttc attcgggtgc tgggccagat aatcctgatg ataagcctcc gcatcatgga
     3241 aagcgtcgag cggggtcacc tgagtgacga ttgaccgact gaacagtttc aaacgatcaa
     3301 gttgcttgat gtacgcttcc gcaattcgtt tctgttcctc attcgcgtag aaaatagccg
     3361 agcgatactg agtgccgcga tcaggtccct ggcggttcag ttcggtcgga tcgtgggcca
     3421 cggaaaagaa aactttgagc agttgcccgt aagtgatttg cgacgggtcg taagtgacga
     3481 gtactgattc ggcgtgaccg gtcgtacctg aactaacctt gcgataatcg gccgaggcgg
     3541 catcgcctcc ggcatagccg gatttcgcat cgctgacacc cttcacatgt tcgaagacgg
     3601 cttcgattcc ccaaaagcag ccgcctgcga ggactgcctt ctgttcacct ttgacgcttg
     3661 ccagcggggc gtcgacttcg ggatccggaa gcggagtgga cgacgcgcct gcagtgctgc
     3721 aggcgaaggt gaatccaatc aagaacagaa tcgaggttat tagtattgcg gtggtcatag
     3781 cattagtctc aggtttcagg tttcagtttt cagtttcgat tcgagaagcc gttcgctggg
     3841 atgccaacat catactaact tagcagctcc tccacgagtc gatccacgtg tatccgcgtc
     3901 gtatcgaaga aacgaattcc attgcgctgg ccgtctgtaa acgtttccgg actgagcagc
     3961 agcggcaatt cagtaccacc gagaatgacg ccttcaatac cgtctcgctt ttgaagctca
     4021 tcggcgattc ggagcatctg ttcgcgagtt tccggcagaa agatatcgtt cagcaactca
     4081 cccatgtact tttcatggat gaagtcctgt tcttcgtcgt taggagtaac caactgaatg
     4141 ccgttacgcg agaatacatc cggatagaac cggccttgca tcgtgaaacg cgttccgaac
     4201 aggccaatct ttttcagacc aaactcctgc acgcgcgagc acgctgcttc tacgatgctg
     4261 attagtggca gacttgatcg gcgctgcaat tcatcaaaaa caatatgggg cgtgttggag
     4321 gca
//
LOCUS       DASUEX010000341         2574 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_739924, whole genome shotgun sequence.
ACCESSION   DASUEX010000341 DASUEX010000000
VERSION     DASUEX010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_739924"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..898
                     /locus_tag="VFV61_05735"
     CDS             <1..898
                     /locus_tag="VFV61_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900013.1"
                     /translation="VIHLNQPNYVAATNSFVPPLAAGNILATNKNVTGGVHQIQSSSN
                     SRYNSLQVTLTRRYAQGLQMTAAYTYGKSHDYYNGTPVNELASPAGDQFNWKLNYGRS
                     DFNREHRLVISGVYDLPKSTSESDVARALLNNWQIAGIAVFQSGLPFSVIDNPGNAVF
                     SRANFNPAFSGQIQCSGNVSGCLNNYFAQGAFVTSRPRLDGTSVGVVNNPTFDPNAPF
                     GNTPRNFLTGPGQKNVDLSVIKFIPINERFRGEVRAEFFNVFNWVNYANPVNNIAIAS
                     FGRIISASTGPRVVQFAFKLNF"
     gene            1140..1937
                     /locus_tag="VFV61_05740"
     CDS             1140..1937
                     /locus_tag="VFV61_05740"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX4900014.1"
                     /translation="MPQLTIGNVDIHYEVYGDGEPLVLIPGFAAGAWIWFKHVAPLAS
                     KFKVVTFDPRGIGQSSFESEPLTMRLLADDTAALLHGLGIEQAHILGVSFGGFVAQEF
                     ALAYPESTRTLSLCCTSFGGPKHVSPSIETLTAVLSTDGFNTEERIRRNLLPAFSPDF
                     VCNHSEEIEEVIKLRMANPVVEEAYRSQLKAGVGFNAESRVDAIKAPTLVLSGDADRI
                     VPVQNSRNLAQQIPGARLRLIEGGSHLFFIEQPDEFNGIVVEFLTQN"
     gene            1939..>2574
                     /locus_tag="VFV61_05745"
     CDS             1939..>2574
                     /locus_tag="VFV61_05745"
                     /inference="COORDINATES: protein motif:HMM:NF020134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEX4900015.1"
                     /translation="MTYARIIGTGSYVPEKVLTNADLSRILGDDINEFVSQVIGINER
                     HVCAADESTADLAEKASLRALESAGITPEQLDLIILATDTPEHLSPATSVVVQHRIGA
                     KNAGTFDINCACSGFVTALDTASKFIIADSEYKHVLVIGAYAMSKYLDWNDKKTATIF
                     ADGAGAVVLEASDEGPGFLASKLFADGTFADYMGIYAGGTRMPVTEEVLREG"
BASE COUNT          583 a          683 c          707 g          601 t
ORIGIN      
        1 agtaatacat ctcaaccagc cgaactatgt tgcggcgacg aacagcttcg tgccgccttt
       61 ggctgcggga aatattctgg ccacgaacaa gaatgtgact ggtggtgtgc accagattca
      121 aagttcgtca aactcgagat acaactcctt gcaagtgaca ttgacgcggc gctatgcaca
      181 aggtttacag atgacagccg cgtacaccta cggaaagtcg cacgactact acaacggtac
      241 gcccgtgaac gaacttgcga gtccggccgg cgaccagttc aattggaaat tgaactatgg
      301 acgttcagac ttcaatcgcg aacaccggct ggtcattagt ggggtttacg atctgcccaa
      361 gtcgacatcc gaatcagacg tcgctcgcgc attactgaac aactggcaga ttgccgggat
      421 tgcggtgttc cagtcagggt tgccattctc agtcattgat aatcccggaa acgcggtttt
      481 cagtcgggca aacttcaatc ccgcctttag cggacaaatc caatgcagcg gcaacgtctc
      541 cggatgcctg aataattact ttgctcaggg agccttcgta acttcgcgac cgcgcctgga
      601 tggcacgagc gttggtgtcg tcaacaatcc gacatttgat ccgaatgcac cttttggcaa
      661 cacgccgcgt aacttcctaa ccggtccggg acagaagaac gtcgaccttt ccgtgatcaa
      721 attcatcccg atcaacgagc gcttccgcgg cgaagtgcga gcggaattct ttaatgtctt
      781 caattgggtg aactacgcaa atcccgtcaa caacatcgcg attgcgtctt tcggaagaat
      841 tatttcggcg agtaccggcc ctcgtgtcgt tcagtttgct ttcaagctga acttttaagg
      901 ttcggagcct gcgaagcagg cgagagcgta aagcctgggg tgaagcggag cggaacccca
      961 ggctccaggt ccaaacacaa taacagcccg cggagtgggc ggcagcattt ggcggaaact
     1021 cgtctgtcgc ccgcctcgcg ggctctggcc tttgtgggtt tgagcgtcct ggggttccgc
     1081 tccgtttcac ccaaggcttt acgctttcgc ctgctccgca ggctgggtta ttcaaacgca
     1141 tgccacagtt gactatcggt aacgttgata tccactacga agtttatggc gatggtgaac
     1201 ccctcgtact catccccggt tttgctgccg gcgcatggat ttggtttaag cacgtcgctc
     1261 ccttggctag caaatttaag gtcgtaacct ttgatccgcg aggtatcggg cagtcgtcat
     1321 tcgagtcgga gccgttgacg atgcgactgc tggccgatga caccgccgct ttgctgcacg
     1381 gtctcggaat tgagcaggcg catatcctcg gcgtgtcttt cggaggattc gtagcgcagg
     1441 aattcgcgtt ggcttatccc gaatcgactc gcacactttc actttgctgt accagttttg
     1501 gcgggccgaa acatgtatct ccctcgattg agacattgac ggcggtcctc tcgacagatg
     1561 gcttcaacac cgaggagagg attcgacgca atctgttgcc agccttctcg cccgattttg
     1621 tgtgcaacca ttccgaagag attgaagagg tgatcaaatt gcggatggca aatccggtgg
     1681 tagaagaggc ttaccggtcg caattgaaag ctggagtcgg attcaatgct gagtctcgag
     1741 tagatgcgat caaagctccg acgctcgtgc tcagcggtga cgctgacagg atcgtgccgg
     1801 tgcagaactc gcggaacctc gcgcagcaga ttcccggtgc gcgtctacgc ttgatcgaag
     1861 gtggcagcca tctgtttttc atcgagcagc cggatgaatt taatggaata gtggttgagt
     1921 tcttaacgca aaactagaat gacctacgcc cgcatcattg gaaccggttc gtatgtgccc
     1981 gaaaaggtgc tgaccaacgc cgacctgagt cgcatactcg gtgacgacat caatgagttt
     2041 gtttcccaag tgattggcat aaatgaacgc cacgtctgtg ccgctgacga aagcaccgct
     2101 gacctggctg aaaaggccag tttgagggcg ttggaatctg cagggataac gcctgaacaa
     2161 ctcgatctga tcatcctggc gaccgacact cctgaacatc tgtcaccagc gacctccgtt
     2221 gtggttcagc atcgcatcgg cgcaaagaac gctgggacat tcgacattaa ttgcgcgtgc
     2281 tccggcttcg tcactgccct ggatacggcg tcgaagttca tcattgctga ttcagaatac
     2341 aaacatgtgc tggtgatcgg cgcctacgcg atgagcaagt atctcgattg gaacgacaaa
     2401 aagacggcaa caattttcgc tgatggcgcc ggcgcggtcg tcctcgaagc aagcgacgaa
     2461 ggacctggct tcctcgcttc aaagttgttc gctgatggaa cgttcgctga ttacatgggc
     2521 atctatgcgg gcggtactcg catgccggtt acggaagaag tgttgcgtga aggg
//
LOCUS       DASUEX010000342         2388 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_987349, whole genome shotgun sequence.
ACCESSION   DASUEX010000342 DASUEX010000000
VERSION     DASUEX010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2388
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_987349"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="VFV61_05750"
     CDS             <1..427
                     /locus_tag="VFV61_05750"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HEX4900016.1"
                     /translation="IVAAAENGDEVLQVASFAAWNHAVGEGLRKNAVPVKLEGDRLLV
                     AVPDAIWQKQLQPLVNQLIFKLNSALGRPLIRAIDLKLEPQSFASRATSDAAGISKTQ
                     AFITPELLSAAARISDPALRRVFIGAAENCLHRLEQAKE"
     gene            434..730
                     /gene="gatC"
                     /locus_tag="VFV61_05755"
     CDS             434..730
                     /gene="gatC"
                     /locus_tag="VFV61_05755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEX4900017.1"
                     /translation="MPINEADIEKIAALAHMDITPEELKVFVPQMAGIVAYVEQLNEV
                     DTSDVEPALGGLTPAGEQTDSSRDDEVSPSLGQKVALAEAPDAANGHFRVPKVL"
     gene            781..2238
                     /gene="gatA"
                     /locus_tag="VFV61_05760"
     CDS             781..2238
                     /gene="gatA"
                     /locus_tag="VFV61_05760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEX4900018.1"
                     /translation="MEETRSEISNLRNRISSGEVNVRTVVESSLTAAERLNETLNAFL
                     AIDRAGSKRRADQLDGDAGNSEPLAGIPIAIKDNICVRGMQTSCGSRILGDYHPPYNA
                     TVVDRLLDAGAVIIGKTNCDEFAMGSSNENSAFGPVRNPWDTSRVPGGSSGGSAAAVA
                     AGIVPVALGSDTGGSVRQPASLVGVVGLKPTYGRNSRYGLVAFASSLDQVGIFGRTVA
                     DVATVLRVIAGRDSHDATTADVPVPDYHLDLGQSIKGARIGFPRALFGEGLDTEVRES
                     VEHAVETYRELGAEIVDVDLPNAKYAIAVYYIIATAEASSNLARFDGVRYGFRAEDAP
                     ELRQMYRKTRDEGFGQEVKRRIMLGTYVLSAGYYDAYYRKAQQVRSLIKNDFGHAFKS
                     CDAIITPTSPTTAFRIGEKVDDPLAMYLNDIYTVTANLAGVPGINVPCGLSSDKLPIG
                     FQLLGPYWSEPTLFRLAHAYEQARPFTEHPPIFTG"
BASE COUNT          528 a          650 c          669 g          541 t
ORIGIN      
        1 gattgtcgcc gctgcggaaa atggcgatga ggttttgcaa gtggcctctt ttgcagcctg
       61 gaaccatgcg gtgggcgagg gtctgcggaa aaacgccgtg ccggtaaaac tcgaaggaga
      121 tcgtctgctc gttgccgtcc ctgatgcgat ctggcaaaaa cagttgcagc ccttagtcaa
      181 tcagttaatc ttcaagctca attcagctct tgggcgaccg ctgattcgag ccattgattt
      241 gaaactggaa ccgcagagct ttgccagccg agcgacttct gatgctgcgg gaatcagcaa
      301 aacccaagct ttcatcacgc cagaactact ttcggcagca gcgagaatca gtgaccccgc
      361 attacgtcgc gtattcatcg gtgctgccga aaactgcttg caccggttag agcaggcaaa
      421 agagtaatcc tttatgccaa ttaacgaagc cgacatagaa aagatcgcag cgctcgccca
      481 catggatatc acgccggagg agctgaaagt atttgtgcct cagatggccg ggatcgtcgc
      541 atacgtcgag cagttgaatg aagtggatac gagtgatgtc gaaccggctc tcggtggtct
      601 aaccccggcc ggagagcaga ctgactcttc gcgggacgac gaagtaagcc catcattggg
      661 ccagaaggtg gcgctcgccg aggcaccgga tgccgccaat ggtcactttc gcgtaccaaa
      721 ggtgctgtga aaaggatctc tgccgattag tcttgactct cgcgcgctca ggaaagcaac
      781 gtggaagaaa ccagaagcga aatatctaac ctgcgaaacc gcatttcaag tggtgaggta
      841 aacgtccgca ccgttgtgga gtcttcgtta accgccgcag agcggctgaa tgaaactctg
      901 aacgcgtttc tcgcaattga tcgagctggc tcaaagcggc gtgcggatca gctcgatggc
      961 gatgcgggaa actctgagcc cctggcaggt atacccattg ccatcaaaga caacatctgc
     1021 gtccgcggga tgcaaacatc ttgtggctcc agaattctcg gtgactacca tcctccctac
     1081 aacgcaaccg tagttgaccg gctcctcgat gccggtgcag tgatcatcgg caagaccaac
     1141 tgcgatgagt ttgcgatggg ctcttcgaat gagaattccg cgtttggtcc agtcaggaat
     1201 ccctgggata ccagtcgcgt accaggtggt tcctcgggag gatctgcggc tgcggtcgct
     1261 gccgggattg tgcctgtagc cttgggttct gacactggag gttcagtgcg tcaaccagct
     1321 tcgctcgtcg gcgttgtcgg attaaaacca acctacggcc gtaactctcg ctatggactg
     1381 gtcgcgtttg cctcttcgct ggaccaggtg ggaatttttg gccggacagt ggcagatgtg
     1441 gcgaccgtct tgcgagtaat tgccggtcgc gattctcatg atgcaactac cgccgacgtg
     1501 cccgtcccgg attaccacct tgatttgggt cagtcaatca agggcgcgcg catcgggttt
     1561 ccacgtgcgc tatttggcga gggtttagac acggaagtcc gcgagagtgt cgagcatgct
     1621 gtggaaacct accgggaact gggggctgaa attgttgacg tcgatctgcc aaacgccaag
     1681 tatgcgatag ctgtttacta catcatcgcc accgcggaag catcttctaa cctggcccga
     1741 ttcgatggcg tacgctacgg atttcgcgcc gaggacgcac ctgaactaag acaaatgtac
     1801 cgcaagacgc gtgacgaggg gtttgggcag gaggtgaagc ggcgcatcat gcttggcacc
     1861 tatgtgcttt cggccggtta ttacgacgcg tactatcgga aggcccaaca agtgaggtcg
     1921 ttaatcaaaa atgacttcgg acatgccttt aagagttgcg atgcaatcat cacgcctact
     1981 tcaccgacca ctgcttttcg catcggtgag aaggttgacg atccgctcgc catgtatttg
     2041 aacgatatct acaccgtgac tgcaaatctg gccggcgttc ccggtatcaa tgtcccctgc
     2101 gggctttcca gcgataagct gcctatcggc tttcaactgc tcgggcctta ttggtcagag
     2161 cctactcttt tccggctggc gcacgcctac gaacaggctc ggccatttac tgagcacccc
     2221 ccgattttta cgggataaat tttagttgag ctagtgaaaa ccttgaggcg gggtgaaccg
     2281 acctttccga cctgtgagct ttcgaaaccc tggggttgct gcatcacgcc cgctgcccac
     2341 cggcccctat cgatcctgcc catcatttcc tgagctatcg actggcgc
//
LOCUS       DASUEX010000343         2332 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1193416, whole genome shotgun sequence.
ACCESSION   DASUEX010000343 DASUEX010000000
VERSION     DASUEX010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2332
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1193416"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1307
                     /locus_tag="VFV61_05765"
     CDS             <1..1307
                     /locus_tag="VFV61_05765"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HEX4900019.1"
                     /translation="TAYRAIRKLEPGHSLCWRKGEIKLERYWQPDFSQKLDISEEEAG
                     ERTLEILRDAVRVRLMSEVPLGAFLSGGIDSSAVVALMSEASSEPVKTFSIGFEEQDF
                     SELHHARRIAEHVGADHHEFIVRPDAMEVLPLLVEHYGEPYADSSAIPTYYVARETRK
                     HVTVALNGDGGDESFAGYERYAAMQLAERYHRLPASLRKLAIQPAIGLLPSSETSRSR
                     IRDAKRFIQAASLPKRERYLRWVSVFDAQAKQELYSADFQKQTANVHAAEILDPWFAR
                     ANGSGIVDAALLTDIMTYLPNDLLVKVDIASMAVSLEARSPFLDHHVIEFAASLPERF
                     KLRGLTTKYLLKQMLKKLLPAENLERRKMGFGVPIGKWFRDEMQSFLRETLLSEKALG
                     RGLFKPEAVKQMIELHTRGERDYAHQLWTLLMLELWFDRFID"
     gene            1558..2319
                     /locus_tag="VFV61_05770"
     CDS             1558..2319
                     /locus_tag="VFV61_05770"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEX4900020.1"
                     /translation="MKGIVLAGGLGMRLRPLTAVTNKHLLPVYDQPMIYYPIQTLVNA
                     GITDIMIVTGGNSAGDFLKLLGNGKAFGLKHLNYTYQEGEGGIAEALALVEHFAAGQP
                     VCVVLGDNIIEGNIAAAVRAYAEQKRGAKILLKRVPDPQRFGVPELDGDKVLSIEEKP
                     KLPKSEYAVIGIYMYDAAVYDIIRTLKPSGRGELEITDVNNAYIARGEMSWQELDGWW
                     TDAGTFDSLRRASNLVAETGANKAEIADCRSAIAD"
BASE COUNT          552 a          590 c          648 g          542 t
ORIGIN      
        1 taaccgctta tcgcgccatc aggaagctgg aaccaggtca ctctctctgc tggcgcaaag
       61 gtgaaatcaa actcgaacgc tattggcagc cggatttttc acagaagctc gacatcagcg
      121 aggaagaggc gggcgaacgc actctcgaaa tcctgcgcga tgcggtccgt gtgcgtttga
      181 tgtcggaagt tcccctgggt gcgtttctat cgggtggcat cgattccagt gctgtggtcg
      241 ccttaatgag cgaggcatcg agcgagccgg tcaagacatt ttcgattggg tttgaagaac
      301 aggatttcag tgaactgcat catgcgcgtc gcatcgccga acacgttggc gccgatcacc
      361 acgaattcat cgtgcgcccg gatgcaatgg aagtgctgcc actcctggtc gaacattacg
      421 gcgaaccata cgccgactct tcagcaatcc ccacttatta cgttgcgcgt gaaacccgca
      481 agcacgtgac tgttgccctt aatggcgatg gtggggacga atcgtttgcc ggctatgaac
      541 gttacgcggc catgcaattg gccgagagat atcatcgcct gccggcatcg ttgcgcaagt
      601 tggcaattca accggcgatc ggactcttgc cttcgtcaga gacaagccgc agccggattc
      661 gcgacgcgaa gcgtttcatt caggcggcct cgttaccgaa gcgcgaacgc tatttgcgct
      721 gggtaagtgt gtttgacgcc caggctaaac aagagctcta ctccgcagac tttcaaaaac
      781 aaaccgccaa cgttcatgcg gcggagattc ttgatccctg gtttgcgcgg gcgaacggat
      841 cgggaatcgt cgatgcggcg ttgctgacgg acatcatgac ttatctcccc aacgatctgc
      901 tggtcaaagt agacattgcc tcgatggcag tttctcttga agctcgttca ccctttcttg
      961 atcatcatgt gatcgaattt gctgcgagtt tgccggagcg cttcaagcta cggggtctga
     1021 ccaccaagta cttgttgaag cagatgctga aaaagctttt gccggccgag aatctcgagc
     1081 gtcggaagat gggttttggc gtccccattg gtaaatggtt ccgcgacgag atgcagtctt
     1141 tcttgcgtga gactttgctg tccgagaagg cactcggccg cggacttttt aaaccagaag
     1201 ctgtgaagca aatgatcgaa ctacacacgc gtggtgaacg cgactatgcc caccagctct
     1261 ggacgttgct aatgctggaa ttgtggtttg atagattcat tgattgaatt tcgtcatggc
     1321 gtttcatttt tgaacttggt gctttgaact tagaactttg aacttggtgc tttgaactct
     1381 gtactttgtg cttcgcgtct tatgtcgtga cgcatcactt cgagagaact attcagcaaa
     1441 gagatagaac aaagtacaaa gttcaaagca ccaagttcaa agcaccaagt tcaaagctca
     1501 aagttcaaag ttcaaaaaac gaatactgaa aagaaacatg gcaacgtgga ggatcaagtg
     1561 aaaggcatcg ttctagctgg aggtttggga atgcgcttgc ggccgctgac agcggtcacg
     1621 aataagcatc tccttcccgt ctacgaccag ccaatgatct actatccgat tcagacgctg
     1681 gttaacgccg gcattacaga catcatgatc gttaccgggg gaaattccgc aggggatttc
     1741 ttgaagttgc tgggaaacgg caaggcgttt ggtctgaagc atctcaatta cacttatcag
     1801 gaaggtgaag gtggcatcgc cgaagcgctg gcactggtgg aacacttcgc cgctggccag
     1861 cctgtctgcg tagtgctggg cgacaacatc atcgaaggca atatcgctgc ggcggtgcgt
     1921 gcctacgccg aacagaaacg cggcgcaaag atcttgttga agcgggtccc ggacccgcaa
     1981 cgctttggcg tccctgagct ggatggtgac aaggtgttga gtattgagga gaagccgaag
     2041 ttgcccaagt cggaatatgc cgtcatcggg atctatatgt acgacgctgc ggtctacgac
     2101 attattcgca ccttaaaacc ctcgggccgc ggcgagcttg agattactga cgtgaacaac
     2161 gcctacatcg cccgcggtga aatgtcatgg caggaactcg atggctggtg gaccgacgcc
     2221 ggcaccttcg atagcctgcg ccgcgcttca aatctggtgg ccgagaccgg agccaacaag
     2281 gccgagattg ccgattgccg atcggcgatt gctgactaaa aaggattgtc aa
//
LOCUS       DASUEX010000344         2029 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1438930, whole genome shotgun sequence.
ACCESSION   DASUEX010000344 DASUEX010000000
VERSION     DASUEX010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1438930"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1463)
                     /locus_tag="VFV61_05775"
     CDS             complement(<1..1463)
                     /locus_tag="VFV61_05775"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF037667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEX4900021.1"
                     /translation="MACACGNRLTGFNTQEILATSDGHAVKDLFSKPIMATFFNWRTH
                     LLIALSMLMLGASALSAQTNQPAPNDPQTATPPGPSRADLLRGEYGPFRSNNDLLFYH
                     LDVRVDPEKKFISGKNTIRFKMLKDDSRIQLDLHEALKVDKILFGKTALKFERDSGAV
                     FVDFPVTLRRGHNYEIDFYYSGNPLEAGRFGGIAFRRDPFGRPWINTACEGEGASIWW
                     PNKDQWRDEVESMRLSVSAPNGLTDVSNGKFLGKTDLGDGYTRWDWLIHYPINNYNVS
                     LNIGKYDHFSDRLGDLTLDYYVLPENLEKAKKQFSQTRGMLEAFQHYFGAYPFKADGY
                     KLIEAPYSGMEHQSAVTYGNHFANGYLERDWTGVGISTKFDFIIIHESGHEWFGNSVT
                     AADRSDMWIHEGWTTYLECLYVEYMFGKQDGLKYTNGYKSKVHNREPIIAPRGVNATP
                     PQDMYFKGALFLNTLRNVVDDDRQWWSLIRGLYQQFK"
     gene            complement(1565..>2029)
                     /locus_tag="VFV61_05780"
     CDS             complement(1565..>2029)
                     /locus_tag="VFV61_05780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900022.1"
                     /translation="TGHRLDMPRCDGLSFELQAVSLKAPIAGVYDLMQFMPANVPRID
                     GSIGLDVLAGRSITLSLARKQLILESRGSLAARIRRGKEISIRLVRDAEGVALSIVVA
                     VVTPKGLAWMEIDSGNGGATVIGKHIAPLFNLKSGTKELHPLKMELAGGIPV"
BASE COUNT          454 a          547 c          535 g          493 t
ORIGIN      
        1 tacttgaact gctggtagag gccgcggatc aatgaccacc attgccgatc atcatccacc
       61 acgttgcgga gcgtattaag gaaaagtgcc cctttgaaat acatatcctg cggcggtgtc
      121 gcattcacac cacggggtgc gatgatcggt tcgcggttat gtacctttga tttataacca
      181 tttgtgtact tcaggccgtc ttgcttcccg aacatgtatt ccacgtacaa gcattcgaga
      241 taggtggtcc atccttcatg gatccacata tcagagcgat cagcggctgt gacgctgtta
      301 ccgaaccatt cgtggccgct ttcatggatg attatgaagt cgaactttgt actgatgccg
      361 acgccggtcc aatctctttc cagataaccg tttgcaaagt gattaccgta agtcacagcg
      421 ctctggtgtt ccatgccgga gtagggggct tcgatcaact tgtaaccgtc tgctttaaat
      481 gggtaggcgc caaaatagtg ctggaaagct tcaagcatgc ctcgcgtctg cgaaaactgc
      541 tttttcgctt tctcaaggtt ttctggcagc acgtagtaat cgagcgtgag gtcgccgagt
      601 cgatcggaga agtggtcata tttgccgatg ttcagtgaga cgttgtagtt gttgattggg
      661 tagtggatca gccagtccca gcgtgtatag ccgtcgccaa ggtcggtttt gccaaggaac
      721 ttcccatttg aaacatccgt caggccattc ggagccgata cgctgaggcg catactctca
      781 acctcatcgc gccactgatc cttgttgggc caccagatgc tcgcgccttc gccttcacag
      841 gcggtattaa tccacggcct gccaaaaggg tctcggcgaa atgcgatgcc accaaacctg
      901 cctgcttcca gcgggtttcc cgaataataa aaatcgattt cgtagttgtg gccacggcga
      961 agcgtcacgg ggaaatcgac gaagacagca ccggaatccc gttcgaactt aagcgcagtc
     1021 ttaccaaaaa gaatcttatc gaccttgagt gcctcgtgca ggtcaagttg aattcgggaa
     1081 tcatccttca acattttgaa gcggatggtg tttttgccgc tgataaattt cttctccgga
     1141 tcgactcgta cgtcaaggtg gtagaagagc agatcattgt tagaacgaaa tggtccgtac
     1201 tcgccgcgta gcaggtcggc gcggctgggc cctggcggtg tcgcagtttg cggatcgttg
     1261 ggcgctggtt gattggtctg tgccgagagc gccgatgcgc cgagcattag catgcttaac
     1321 gcgatgagca gatgagtccg ccagttaaag aaggtcgcca tgattggttt cgaaaacaga
     1381 tcctttacgg cgtggccgtc cgaagtcgca aggatctcct gtgtattaaa gcccgttagt
     1441 cggtttccgc aggcgcaagc catgcgcggc ccttcgcgag atccacggtc aagtcccagt
     1501 tttgcaggaa ccgcgtccct atattgccat ccataatcag tgtgtcgttt acgcgcgcat
     1561 tgccttagac cgggatgccg ccagcgagct ccatttttag ggggtgcaac tccttggtgc
     1621 ctgatttcag gttaaacaaa ggcgcgatgt gctttccgat gaccgtcgca ccaccatttc
     1681 cggaatcaat ctccatccac gccaagcctt tcggcgtgac gacagcgaca acgatactga
     1741 gcgcgacgcc ttcagcgtca cgaacaagcc taatagagat ctccttgccg cgcctgatcc
     1801 gagccgccag actaccgcga ctctctaaaa tcagttgttt ccttgccagg ctcagcgtaa
     1861 tagaacgccc cgccagaaca tcgagaccaa ttgagccgtc gattctcggc acgttggccg
     1921 gcatgaactg catgagatcg tagacgcccg caatcggggc tttcaaactc actgcctgga
     1981 gctcaaaact cagaccatcg caacgaggca tgtccagtcg atgtccggt
//
LOCUS       DASUEX010000345         2423 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_634922, whole genome shotgun sequence.
ACCESSION   DASUEX010000345 DASUEX010000000
VERSION     DASUEX010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_634922"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1292)
                     /locus_tag="VFV61_05785"
     CDS             complement(<1..1292)
                     /locus_tag="VFV61_05785"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4900023.1"
                     /translation="MGETDTDAADQFDARITPTKLDEGTPGLWRRYRRLPRNVFAISL
                     VSLLNDASSEIIYPLLPIFLSLTLGASPGIVGVIEGAAESISSLLKLFSGYFSDRRGK
                     RKGFVVLGYSLASFARPLLAFASSWYQVLAIRLTDRVGKGIRSAPRDAMIADTVAVEE
                     RGLAFGFHRAMDHTGAVAGPLFGYLLLATFAANRNAPSAADFTKIFLLASIPALAAVV
                     VVTFFVRESPRASDRHESVVTPPSSLSLRGFDGNFKRFLLIIALFTLSNSSDAFLLLR
                     AQSAGVSIASIPLLWAMLHVVKVVSSLIGGDLSDRLGRRRLIVSGWILYAAVYAGFSF
                     VSNKISVWILFLIYGIYFGLAEGAEKALVADLVRPEQRGTAYGLYNLAFGITVLPASL
                     LMGALWSWRGPTLAFLVSAGLGASAAVLLLLLVKQPIT"
     gene            complement(1302..1529)
                     /locus_tag="VFV61_05790"
     CDS             complement(1302..1529)
                     /locus_tag="VFV61_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900024.1"
                     /translation="METSTIYIIGAVVLLAIFVLIARLAVRWLVRLTMMGVIIIALVG
                     GALFWWWTRSLSEQAPPARPHPTPSKRAANR"
     gene            complement(1663..2079)
                     /gene="queF"
                     /locus_tag="VFV61_05795"
     CDS             complement(1663..2079)
                     /gene="queF"
                     /locus_tag="VFV61_05795"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR03139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HEX4900025.1"
                     /translation="MAAGKPTAINQQGLDIRSTPRAEMDLYPLDTFPYEFTGREIKIN
                     FEIPEFTAICPFSDFPDFAVIRLEYVPNQRCVELKSLKLYINSFRNVKIFHEHVINVI
                     LEDFVRACDPLSADIEGDFHIRGNIKTVVRASYRKK"
BASE COUNT          608 a          658 c          668 g          489 t
ORIGIN      
        1 tgagtaatag gttgcttcac cagtaatagc aacagcaccg ctgctgaagc acccaacccg
       61 gcgcttacaa gaaacgcgag ggtgggaccg cgccaactcc aaagcgcccc catcagcaaa
      121 gatgccggca gtacggttat accgaacgcg agattataca aaccgtaggc ggtgccgcgt
      181 tgttcaggcc gcaccagatc cgccaccaac gctttctcag ccccttcggc caaaccaaaa
      241 tagatgccgt aaattagaaa caggatccag actgaaatct tgttagacac aaacgaaaac
      301 cccgcgtaca cagcagcgta aaggatccat cccgaaacaa tcagccggcg tctgcccagg
      361 cggtccgaga gatcaccgcc aatcaaagag ctcaccactt tgacaacatg cagcattgcc
      421 cacaacaacg ggatcgaagc gatggaaaca cccgccgatt gtgcccgtaa aagcagaaac
      481 gcgtccgacg agtttgaaag tgtgaaaaga gcgatgatta ataggaaccg cttgaagttg
      541 ccgtcgaagc cgcgtaacga aagtgagctc ggaggagtca caacactttc gtggcgatca
      601 ctcgcgcgcg gtgattcacg cacaaagaaa gttacgacca ccacggctgc gagcgcagga
      661 atcgaggcca gtaagaatat tttcgtgaag tccgctgcgc tgggcgcatt gcgattcgcc
      721 gcgaatgttg ccaacagcaa gtatccaaac aacggcccgg ctaccgcgcc ggtatgatcc
      781 atggcccggt ggaacccaaa agccagcccg cgctcctcaa ccgcaactgt gtcagcaatc
      841 atcgcgtcgc ggggcgcgct acgaattccc tttcccacac gatcggttag gcgaatggcc
      901 agcacctgat accaactgct cgcaaaagca aggaggggcc gggcaaagct ggcaagcgag
      961 tatccgagaa ccacaaaacc ctttcgcttg ccgcgtcgat cgctgaaata tccggaaaag
     1021 agtttgagta aactggagat tgattcggcg gcgccttcaa tcacgccgac gattcccgga
     1081 ctcgctccca gagtcagaga aaggaagatt ggaagtaacg gatagatgat ctcgcttgag
     1141 gcgtcgttta gcagactgac gagactgatg gcaaagacgt tgcgcggcag gcggcgataa
     1201 cggcgccaga ggccaggagt gccctcgtct agttttgttg gagtaatgcg cgcatcaaat
     1261 tgatcggcag cgtcggtatc agtttccccc atgatcgctc gttaacgatt agctgcgcgt
     1321 ttactgggcg tggggtgggg cctggctggg ggcgcttgtt ctgacaagct gcgagtccac
     1381 caccaaaata atgcaccacc cacgagggcg atgatgatta cgcccatcat cgtcaaccgc
     1441 accagccagc gcaccgcaag tcgcgcgatc aagacaaaga tcgccagcaa gacgacggcg
     1501 ccgatgatgt atatggttga ggtttccaat gggtttcaag tgtcgggttt caggtttcag
     1561 gttgaatcga aatgttgccc gtaagcggcc ccggtttagg tcttagcctt cgtttttcag
     1621 aggatgcctc tggtccgaaa cccgacaccc gatatctgaa acctattttt ttcggtagct
     1681 tgcccgcacc accgtcttga tattcccacg aatgtgaaag tctccttcaa tgtcagcgct
     1741 caagggatcg caggcacgaa cgaagtcttc gagaatcacg ttaattacat gctcgtggaa
     1801 gatcttcaca ttgcgaaatg aattgatata gagcttcaag ctcttcaact ccacgcagcg
     1861 ctggttgggc acgtactcga gccggatgac cgcaaagtct ggaaaatccg agaaggggca
     1921 aatcgccgta aattccggga tttcgaagtt gatcttgatt tcgcgaccgg taaactcgta
     1981 agggaaagtg tcgagcggat aaaggtccat ctccgcgcgc ggagtggagc gaatgtcgag
     2041 tccctgttga ttgatcgctg tcggtttgcc tgctgccatg attgtgctca cattacgcag
     2101 cgatcttaac tgaggcttgt cgcagacacc aaactcacat tctattggcg attgccgatt
     2161 ggcgattgcc aattcggcgc ggagcggatt gaagccgcta ttccgacagg tcggtgaagc
     2221 ctgggtccga ttaccaatgc gggattgtgg attgacgatt ggttggaggc agtaccacca
     2281 ggcggtagcc cggtgggccg tcacattgcc gattgccgat tgcggattcc cagggcctga
     2341 gtcgctaggt ggtgacggtt gggcttcgtc cgatggtaaa aactttcacc agcgctacac
     2401 cggccacaaa cccgccaatg tgg
//
LOCUS       DASUEX010000346         2807 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_70285, whole genome shotgun sequence.
ACCESSION   DASUEX010000346 DASUEX010000000
VERSION     DASUEX010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70285"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(194..1060)
                     /gene="dapF"
                     /locus_tag="VFV61_05800"
     CDS             complement(194..1060)
                     /gene="dapF"
                     /locus_tag="VFV61_05800"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEX4900026.1"
                     /translation="MPPSFIKFHGFGNDYIVIEAERLAPIASLGNFARQICNRHYGAG
                     ADGIAVVSPSLEVSADFRVRIFNPDGSEAALSGNGTRCAAAYLFYQNLWEADELRLHT
                     RSGIKRYFIREVPQPGKFIFDSELGRPRFASDEIPMQTEERLEQVVNYPLAVGDQTVR
                     VTALEMGNPNCCIFVDNFSDLDWRALGPQIENHAQFPDRTNVTFVQVRDRSHIELRIW
                     ERGVGETTASGSCSCAAAVAAMVNGKAERRVNVVTPGGTVKIHWREDDEVVITGSAEV
                     AYAGDWLGTLET"
     gene            complement(1068..1580)
                     /locus_tag="VFV61_05805"
     CDS             complement(1068..1580)
                     /locus_tag="VFV61_05805"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX4900027.1"
                     /translation="MSVDMLVNTWKEFRKGLIAEVEQIPEDKFDFRATPETRTIAELL
                     QHVVEVQKMLIGEAAHAEANLRRQSFADHVKEYAPEVRTVTDKNGLLEMLRTSMEVCE
                     ASIRSNDDLMQGSMGGIDGKPTPKLKVVNFAMSHEMYHRGQLTVYERLMNLEPALTRH
                     FARMTAQATG"
     gene            complement(1603..>2807)
                     /gene="dacB"
                     /locus_tag="VFV61_05810"
     CDS             complement(1603..>2807)
                     /gene="dacB"
                     /locus_tag="VFV61_05810"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HEX4900028.1"
                     /translation="AGVLRGDLTIFGRGDPSIAARFNSGDYFKGINDLAAQIAAAGVK
                     RVEGDIVGDETYFDGAPYGSGWNWEDLQWWYGAEVSSLTVNDNALDLSIKPGAEVGQP
                     AVITTGPPDPLLKIVNRVVTAPRSSRRDLNMYRTLNSDTLEIAGSLPLDDRGFTAGIG
                     ISHPALLFVYLLRTALGQRGITVTGKSRTIPPASFLSLQPAPESPTRVEIASMQSPPF
                     SLVAAQTLKPSQNLYTEIILRTLGKTVGPTATATNNIRTNEAAGLEVVKAFLKEAGVN
                     LSPLVLTDGSGLSRNDMITAEASVQLLTYMSRHRFASAFREALPIAGVDGTLRNRMKG
                     TVAENNVRAKTGSLSSAATLSGYVTTAAGENLVFSIMVNNYPEDTEVRALCIDPIAVL
                     LASFAGKS"
BASE COUNT          651 a          816 c          705 g          635 t
ORIGIN      
        1 ccggcactgg tgccggcacc gagtgaatta cttcggcttc cgtcttctgc agaacttgtc
       61 cgccacaacc ggcgagaata aaaaccaaca ccagcaccgg gatggcaagc aagaagcaaa
      121 aagtagattt ggtttgaatc gggaagagca tttcgtcggg gttagtccaa tcaattcaat
      181 agtggcaaga cggtcaggtt tccaacgttc ccaaccaatc tccagcataa gctacttcag
      241 cactgccagt tatcacaacc tcatcgtcct cgcgccagtg gatcttaaca gtgcccccag
      301 gcgtcacaac attaacccgt ctttccgctt tgccattgac catcgccgcc acggcagcag
      361 cgcaggagca actcccagac gcagtcgttt cacccacccc tcgctcccag atgcgcaact
      421 caatgtgcga acgatcgcgg acttgaacaa aagttacgtt agtacgatcg ggaaactgtg
      481 cgtgattctc tatctgcgga ccaagtgcac gccaatcaag gtctgagaag ttgtcgacga
      541 agatacagca attcgggttg cccatctcga gggccgtaac ccttacggtc tgatcgccaa
      601 cagcaagagg atagttaaca acctgctcca gcctttcttc ggtttgcatg ggaatctcgt
      661 cgctggcgaa gcgcggccta ccaagttctg aatcgaaaat gaattttcct ggttgtggaa
      721 cttcacgaat gaagtaacgt ttgattcccg agcgagtatg caaccgcagc tcatcagcct
      781 cccaaagatt ttggtaaaag agatacgccg ctgcgcaacg ggtaccatta cccgaaagtg
      841 cggcttcgct gccatcggga ttgaaaatgc gcacgcgaaa gtctgcgctt acttccagcg
      901 acgggctgac aacggcaatc ccatctgcac cggcaccgta atggcgattg cagatttgtc
      961 ttgcaaagtt gcccagactg gcaatcggtg ccaggcgttc ggcctcgatc acaatgtagt
     1021 cgttgccgaa accgtgaaac ttaatgaagc tcggtggcat tgcgggctca accggtcgcc
     1081 tgggcggtca ttcttgcgaa gtgtctggtc agcgcaggct ccaaattcat cagccgttcg
     1141 taaaccgtca gttgtccccg atgatacatc tcatgagaca tcgcaaagtt tacgaccttc
     1201 aacttcggag tcggtttgcc atcaataccg cccatcgatc cctgcatcag gtcatcattc
     1261 gaacgaatgc ttgcttcgca gacttccatg ctggtgcgca acatctcaag caaaccattc
     1321 ttatcagtga ccgtcctcac ttcaggggca tactctttca cgtgatctgc gaacgactgg
     1381 cgtcgcagat tggcttcggc gtgcgcggcc tcaccaatca acattttctg cacttcgacg
     1441 acatgctgaa gcagttccgc aatcgtgcgc gtttctggcg tggcgcgaaa atcaaacttg
     1501 tcttcgggaa tttgttccac ctcagcaatc aaccccttgc gaaactcctt ccaggtgttt
     1561 accagcatat cgactgacat ggcttctcct tttgattaga ggttacgatt tgccggcgaa
     1621 cgatgccagc aacaccgcaa tcggatcgat acaaagcgct ctcacttcag tgtcttccgg
     1681 gtaattgttc accatgatag aaaaaacgag gttctcgcca gccgcggtgg tgacgtaacc
     1741 agataatgtc gccgcggaac taagcgagcc ggtcttggcg cgcacgttgt tttcggcgac
     1801 tgtgcctttc atccgatttc gcaaagtccc atcaactccg gcgatcggca acgcctcccg
     1861 aaaagcactc gcaaaacgat ggcggctcat ataggtcaac agttgcacag atgcctccgc
     1921 ggtgatcata tcgttgcgcg acagaccaga accatccgtt aagactagcg gactcaaatt
     1981 aacccccgcc tctttcaaaa atgcttttac cacttcaagt cccgcggctt cgtttgtccg
     2041 aatattgtta gttgccgtgg ctgttgggcc cacggtcttc cccaaggttc gcagaatgat
     2101 ttccgtgtaa agattctgac tgggcttcaa tgtttgcgcc gccaccaggc taaacggtgg
     2161 cgactgcatg ctggcaatct caactcgggt tggagattcc ggcgccggct gcaaactaag
     2221 aaaactcgct ggaggaattg tccgcgattt gccggttacg gtgatgccgc gctgaccaag
     2281 cgcagtccgc agcagataga caaacaacag cgcgggatgc gagatgccga ttcctgcagt
     2341 gaagccgcga tcgtccagtg gcaaagagcc ggcaatttcc agggtgtctg agtttagcgt
     2401 ccggtacata ttcaaatcgc gtctgctgct ccgcggcgcc gtaaccactc ggttcacaat
     2461 cttcagtaag ggatcgggcg ggccagtagt gattaccgcc ggttgcccaa cctcagctcc
     2521 cggcttaatc gaaaggtcca atgcgttgtc gttgactgtt aatgaactaa cctcggcgcc
     2581 gtaccaccat tgcaggtcct cccagttcca cccggagcca tagggcgcgc cgtcaaagta
     2641 ggtttcatca ccaacaatgt ctccctcgac gcgcttgacg cccgcagccg caatctgtgc
     2701 ggcgagatcg tttataccct tgaagtaatc gccgctgttg aagcgcgctg cgattgacgg
     2761 atcgccgcgg ccgaaaatcg tcaggtcgcc gcgcagcacg cccgcgg
//
LOCUS       DASUEX010000347         3574 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_70373, whole genome shotgun sequence.
ACCESSION   DASUEX010000347 DASUEX010000000
VERSION     DASUEX010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3574
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70373"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="VFV61_05815"
     CDS             <1..192
                     /locus_tag="VFV61_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900029.1"
                     /translation="ADGRDIAEVYAGLGEKDQVFAWLEKDFQRHDHSMAQLRLEAGLD
                     LVRDDPRFKDLMRRMGLPQ"
     gene            309..1616
                     /locus_tag="VFV61_05820"
     CDS             309..1616
                     /locus_tag="VFV61_05820"
                     /inference="COORDINATES: protein motif:HMM:NF006771.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HEX4900030.1"
                     /translation="MNRRNFSLSLLAAVPAIALRTVQSTSQLRVDGRRVNAHLSELAQ
                     FGKTAEGGTNRVAYSDADLQARQYAMKLMRDARLEVSIDAAGNIVGRRAGSERALKPL
                     MIGSHIDSVPDGGSYDGQVGSMGAIEVAQTLAENNVHLRHPLEVVIFQNEEGGTIGSV
                     AITRGLTEKDLNIVTNSKKTIREGIRFIGGNPDQLASVLRRKGDLAGYLELHIEQGGI
                     LYVEKLDIGIVEGIVGVYWWEVTVAGFANHAGTTPMNQRRDALLAAAKYIDAVNRIVT
                     SIPGRQVGTVGKIQAFPGAYNVVPGKVTTSLGLRDLDAAKVQMVFEKIQAEVSQIERA
                     TGTKFEFKQVNASQPAPTDPRIRNTIAEAAKQLGLSTKLLPSGAGHDAQEIAALCPIG
                     MVFVPSRDGISHSPREFSKAEDITNGVNVLLHSLLKLDALNLT"
     gene            1666..2769
                     /locus_tag="VFV61_05825"
     CDS             1666..2769
                     /locus_tag="VFV61_05825"
                     /inference="COORDINATES: protein motif:HMM:NF010539.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEX4900031.1"
                     /translation="MATSFMSKTAAFMGLRSLRDLVSNSMAIDMGSAATVIYERGRGV
                     VVDEPSLIAVNTISGEVIATGLEAQKIYGREARDVTVIAPMANGVVADFEQTKQMLTY
                     FARKARSGFSHFSRRALMSVLSGITPVERRALLSAAEHAGIGRVWMIEEGLAAAFGSG
                     VRIDDLHASVVVNIGGGTTSVAVVANGAIVHASAERIGGSSINAAIMNHVRRHRGLII
                     GAQSAERLKLELATATLPIDLSHQTSVSGRDVLTGGPAAIEITAGEIYPVAQEVVRKI
                     VDGITASLTELLPEVAGDVYERGIILTGGGSLFGGLADFLREATQLQVRIADEPRYAI
                     VRGLAQLFDEPHWLRHLKQDKVPPLLYAEDELP"
     gene            2815..>3574
                     /locus_tag="VFV61_05830"
     CDS             2815..>3574
                     /locus_tag="VFV61_05830"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX4900032.1"
                     /translation="MNQDLAANASLSHYRILSKLGAGGMGEVYLAQDTKLDRKVALKI
                     LPADVASNRDRMERFVREAKAAAALNHPNIAHIYEIGESDGTHYIAMEFIDGETLRHK
                     IHGAKAPLPKLLKYLIQVAQGLAKAHAAGIVHRDLKPDNIMITRDDYAKVLDFGLAKL
                     VEPQRTFGPNESGSSEIATAVMQQPGLPGRQSNWDSGHSTPGMVIGTIGYMSPEQASG
                     RVDEIDLRSDIFSFGCILFEAATGQRAFAGKDVLD"
BASE COUNT          812 a          927 c         1059 g          776 t
ORIGIN      
        1 gcagatggac gtgacatcgc ggaggtttat gcgggactcg gtgagaagga ccaggttttt
       61 gcgtggttgg aaaaggattt tcaaagacac gatcactcga tggcacaact gcgcctggag
      121 gcgggtcttg atctcgtccg cgacgacccc cgatttaagg atttgatgcg tcgaatgggg
      181 ctcccgcaat gactcttcct gcggtgactg ccgcagttag cagcgaaacg cggtggcctc
      241 gggttgaatt taaagcatcg cgccgttctt cggctcccgc cctttgatcg agttagataa
      301 ctgcacaaat gaatcgtcga aactttagtt tgagtctgct ggctgcggtt ccagctattg
      361 cattgcgaac ggttcaatcg acctcgcaac ttcgcgtcga cggtcgtcgt gtcaacgcgc
      421 atctcagcga gctcgcccag tttggtaaaa ccgcggaggg cggcacaaat cgggtcgcgt
      481 attctgatgc ggatctccaa gctcgtcagt acgcgatgaa gttaatgcgt gatgctcgac
      541 tggaggtgag cattgacgcc gccggaaaca tcgtgggccg ccgcgcggga agcgagaggg
      601 cgctgaaacc gttgatgatt ggatcacaca tcgattccgt gcccgacgga ggcagttacg
      661 acggacaggt cggttcgatg ggggcaatcg aagtggcgca gacgctggct gagaacaacg
      721 ttcatctgag acacccgctg gaagtcgtta tcttccaaaa cgaagagggc gggaccatcg
      781 gcagcgtcgc catcaccaga ggattgactg agaaagacct gaacatcgtc accaacagca
      841 agaaaacaat tcgcgaagga atcagattca tcggtggcaa tcccgaccaa ctcgccagcg
      901 ttttgcgtag gaaaggtgac cttgcaggtt atctcgagtt gcatattgaa cagggcggga
      961 tcttatacgt cgagaaactc gacatcggta tcgttgaggg tattgtcggc gtctactggt
     1021 gggaagtgac tgtggcagga tttgccaacc acgcgggtac gactccgatg aaccagcgtc
     1081 gcgatgcact gctcgcagcc gcaaaataca ttgatgccgt caaccggatc gtcacttcaa
     1141 ttccaggccg tcaggttgga acagtgggga agatccaggc ttttcccggt gcttacaatg
     1201 tcgtgcccgg caaagtcacc acaagtttgg gactgcgtga tttggacgct gctaaggtgc
     1261 aaatggtgtt cgaaaaaatt caggctgagg taagccagat tgagcgcgca accgggacga
     1321 agttcgagtt caagcaggtg aatgcgtctc agcctgcgcc taccgatccc cgtatccgca
     1381 acacgatcgc tgaggcagcc aaacaactcg ggctgtcgac caagctactg cctagcggcg
     1441 cgggccacga tgcccaagag attgcggctc tctgtccgat tgggatggtc tttgtgccaa
     1501 gtcgcgacgg catcagtcat agcccacgcg agttttccaa agcggaagac attacgaacg
     1561 gggtaaacgt tttgctccat tcgcttctta agttggatgc gctgaatctg acttgaagcg
     1621 tgcattccag gtccgatcaa atcacgtcaa gaaaagagca ctgcagtggc aacttcgttc
     1681 atgagcaaga cggcagcatt catggggctg cgatctctgc gagacctggt ctcaaattcg
     1741 atggctatag acatgggatc tgcggccacg gtcatctatg agcgggggcg cggagtagta
     1801 gttgatgagc cttctctaat tgccgtcaat acgattagcg gcgaagtgat agcgaccggc
     1861 ttggaggcgc aaaagattta cggacgcgaa gcccgtgatg tcacagtgat cgcgccaatg
     1921 gccaacggtg tcgttgctga ttttgagcaa acgaagcaga tgctcactta ttttgcgcgg
     1981 aaggcacgaa gcggcttttc tcatttcagt cgtcgcgcac taatgagtgt cttgtctggc
     2041 atcacgccag tggagcggcg cgcgctgctc agcgccgccg agcatgcagg gatcggtcgc
     2101 gtctggatga tcgaagaggg gttggcggcg gcttttggtt ccggtgtccg gatcgacgac
     2161 ctgcacgctt cggttgttgt taatatcgga ggtggcacca ccagcgttgc agttgtggca
     2221 aatggggcaa tcgtccatgc cagcgccgag cgaatcggag ggtcgagcat caacgccgcg
     2281 attatgaatc atgttcgtcg tcatcgtggg ttgattatcg gcgcacagtc agctgagaga
     2341 ctcaaacttg aattagccac cgcaactctg ccaatcgatc tttcacatca gacttcggtc
     2401 agcggacgcg atgttctgac gggaggccct gctgctatcg agattactgc tggtgagatc
     2461 tatccggtcg cccaggaagt agtccgtaaa atagtggatg gcattaccgc gtcgctaact
     2521 gagttgctgc cggaagtagc tggagacgtt tatgagcggg gcatcatcct cacgggaggc
     2581 ggctcactgt ttggaggcct tgccgatttc ttgcgggaag ccactcagct ccaggtccgg
     2641 atagccgacg agccgagata tgcaatagtt cgaggtctgg cgcaactctt tgacgagccg
     2701 cactggctgc gtcacttaaa gcaggataag gtgccaccgc ttctctatgc cgaagatgag
     2761 ttgccctagg taacggtcca cttcgcggcg cggacatcac gtactcaaac ttgtatgaat
     2821 caagacctgg cagccaacgc ttctctgtcc cattaccgca tcctctccaa actcggtgcc
     2881 ggtgggatgg gtgaagtcta tctggcgcag gacacaaagc tcgatcgcaa ggtcgcgttg
     2941 aaaattctgc ctgctgacgt agcgtcgaat cgcgatcgca tggagcggtt cgtccgcgaa
     3001 gcgaaagccg cagccgcgct caaccatccc aacatcgctc atatctacga aatcggcgag
     3061 agtgacggca cgcactacat cgcgatggag tttatcgacg gcgaaacgct ccgccataag
     3121 atccatggag ccaaagcacc actcccgaag ttgctgaagt atctgattca ggtcgcgcag
     3181 gggttggcga aagctcatgc ggccggaatt gttcatcgcg atctcaagcc cgacaacatc
     3241 atgatcacac gcgacgacta cgcgaaggta ttggattttg ggttagcgaa gttagtcgaa
     3301 ccgcaacgaa cttttgggcc caacgagagt ggttcgagtg aaatcgcgac cgcggttatg
     3361 cagcaaccgg gacttccagg cagacagtcc aactgggata gcgggcactc aacgccagga
     3421 atggtaatag gcacgatcgg ctatatgtct cccgagcagg caagtggtcg cgtggacgag
     3481 atcgatctcc gttccgatat cttttcgttt ggctgcattc tctttgaggc ggccaccggc
     3541 cagcgagctt tcgccggcaa ggatgttctg gact
//
LOCUS       DASUEX010000348         2199 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1560577, whole genome shotgun sequence.
ACCESSION   DASUEX010000348 DASUEX010000000
VERSION     DASUEX010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1560577"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..806)
                     /locus_tag="VFV61_05835"
     CDS             complement(<1..806)
                     /locus_tag="VFV61_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900033.1"
                     /translation="MKITKDPKQSLLMPCPPLQQQGSIPVEEDLSPEVRAILGTPAEE
                     GACTRDQPICCGDPRVTAQLTNDQPVGKVLPWRIDNAERGHLIMTPGGPSGLIGGLLS
                     QVQPRQYYSHMAIMTRDRVELRHATTSEDRLKHFPHHEGEEGDPGIGLQLSNLPFIPE
                     DLAEIPTDGFQETALLNGWPGTITQSVERAYVSWHDSPKIKVDGKEVPDPAWAEFDPA
                     SNRHYLINAVTLGPVTMSVQQVDGSEKTQLVFPLVVKPCPRWETEQVRSAL"
     gene            1094..1915
                     /locus_tag="VFV61_05840"
     CDS             1094..1915
                     /locus_tag="VFV61_05840"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX4900034.1"
                     /translation="MKRILSLTLTVLFASVCVVAQCSEADQQKLIAFDKAWGEAGQRG
                     DKAALQNIYADDYLNTSVAGTITKAQAIENAVRAAERRSANPQPAAQTTPDNYIIACT
                     PNSATVTHRNVTVTTVDGKERISYSRSVHFLEKRGNDWRVVSDAGGPLNDAAQLLYME
                     MDWSDADKRRDTAWFERNLADDYFGVSSLTGKLTTKAEDVADVKNRKETYDSAVSSDM
                     QVRVDGNTGVVTGIYHLKGRDDKGQPLDRRIRYTDIYAKKDGRWQVIASQGTLIK"
BASE COUNT          503 a          633 c          607 g          456 t
ORIGIN      
        1 agagcgctac ggacttgctc cgtttcccaa cgtgggcagg gcttcacgac gagaggaaaa
       61 accagctgcg ttttctcact gccgtcgact tgttgcactg acattgtcac gggcccaagc
      121 gtgacagcat tgattaagta atgtctgttg ctcgctgggt cgaactctgc ccacgccgga
      181 tccggaactt ctttcccgtc caccttgatc ttgggactgt cgtgccagga aacgtacgcg
      241 cgctcgacgc tttgtgtgat tgttccgggc caaccgttca ggagtgcggt ctcctggaag
      301 ccgtcggtcg gaatctccgc gaggtcttcg ggaatgaagg gcaagttgga gagctgaagg
      361 ccaattcctg gatcgccttc ttcgccctcg tggtggggga agtgcttcag acgatcctcg
      421 ctggtggtcg catggcggag ctctacgcgg tcgcgcgtca tgatggccat gtggctgtaa
      481 tactgacgcg gctgaacctg agatagtaat ccgccaataa gtccggaggg accacctggg
      541 gtcatgatca gatgaccacg ctcagcattg tcgatgcgcc acggtaacac tttccctacc
      601 ggctgatcgt ttgttagctg agcggtaacc cgcggatcgc cgcaacagat gggctgatcc
      661 cgagtgcatg ctccttcctc ggccggtgtg ccgaggatgg cccggacctc cggcgacaaa
      721 tcttcttcca cgggtattga accctgttgt tgcagaggtg ggcagggcat caacaacgac
      781 tgcttaggat cctttgttat tttcatgaac catacctttg cattgattga gcaatagaga
      841 cggcttcgcg aatgagactg agctcttaaa gcgattggcg gccggtgcgc ggatcacacc
      901 atattttttg cccggcctca aacctcatac cagttgacat caaatccgct atcccgtaca
      961 ttccagcgca ctgcagaccg atatccttcc ggcactttct gctgtttacc tgttttcaaa
     1021 atccgactta aaagcgtgca ccccgtaccg cgccactaag tctccctaac tcgtacggga
     1081 aggagcccag ataatgaaac gaattctgtc cctaactctt acagttcttt tcgcttccgt
     1141 ctgcgttgta gcccagtgca gcgaagccga tcaacaaaaa ctgatagcct tcgacaaagc
     1201 atggggtgaa gccggtcagc gcggtgacaa ggctgccctg caaaacatct atgcggacga
     1261 ctacctgaac acgagcgtcg ccggaacgat cacaaaggcg caggcaatcg agaacgcggt
     1321 cagggccgcg gagcgccgca gcgcaaaccc gcaaccggca gcccaaacta ctccggacaa
     1381 ttacatcatc gcctgcacac ccaacagcgc gactgttact caccgcaatg tcacggtcac
     1441 cacagtcgac ggcaaggaac gaatttctta ctcacgcagc gttcactttc tcgaaaagcg
     1501 cggtaacgac tggcgagttg tcagcgacgc aggcgggccg ctcaacgatg ccgcgcaatt
     1561 gctctatatg gagatggact ggagcgatgc ggacaaaagg cgtgacactg cctggttcga
     1621 gcgcaacctt gctgatgact acttcggcgt cagcagcttg actggcaagc tgaccaccaa
     1681 agccgaagat gttgccgacg tcaaaaacag gaaagagacg tatgattctg cggtttcctc
     1741 agacatgcaa gtgcgagttg acgggaacac aggcgtggtg accggtattt accacctcaa
     1801 aggccgcgac gataagggac agccgttaga ccgtcgcatt cgctacactg atatctacgc
     1861 caagaaagac ggacgttggc aggtaatagc atcgcaggga actctcatca agtagctgtt
     1921 cggatattca ggagctttgc ctcataagtg acccacgact gaggttacgg ctttgccgcc
     1981 ggatgtgagg cgcggctcgc cgcgccgaga gcagtccaga atattcgcgg gctatgcccc
     2041 tggttcgggc ccaatcctta accttattgg gccaaaggcc gcaccacggg catagcccaa
     2101 acatgtaaag ggcacctctc cggcgcggcg agccgcgcct cacatccggc ggcaaagccg
     2161 aaacggacat ggcccatgac tgaggcaatt gcaaggcag
//
LOCUS       DASUEX010000349         3601 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1439668, whole genome shotgun sequence.
ACCESSION   DASUEX010000349 DASUEX010000000
VERSION     DASUEX010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3601
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1439668"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            552..1127
                     /locus_tag="VFV61_05845"
     CDS             552..1127
                     /locus_tag="VFV61_05845"
                     /inference="COORDINATES: protein motif:HMM:NF014176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HEX4900035.1"
                     /translation="MKVFSIFAVLIIAVAFISGLSSVSFDVSAQDAKKPQDTYTLATE
                     AKLGPVTFSHINHTSKNRNLEGTGPSSCIECHHTAQPATEAAKHPPLKTAWPADRTTT
                     LSAEVLAKDPTAVGAVCRDCHARAETKPKLLPENPQVKFSVGTELVTLTNQQAFHRNC
                     AGCHDEIVKTRTDVAPPTSKKCTACHKRVAA"
     gene            1312..1629
                     /locus_tag="VFV61_05850"
     CDS             1312..1629
                     /locus_tag="VFV61_05850"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX4900036.1"
                     /translation="MRVSITRWLVLASAIFAVTFMIASVPARTSSQDDNAALYKSKCF
                     ACHGATAEKKFDATKADDVLIQTVLKGKKAEKPPNMPAYEEKGITAEQATALVQFMKS
                     QKH"
     gene            1750..>3601
                     /locus_tag="VFV61_05855"
     CDS             1750..>3601
                     /locus_tag="VFV61_05855"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEX4900037.1"
                     /translation="MARGPLLTAVLLVLLLCSLFQFADRSEAQSPGPADPAATPAATP
                     LQPAGQTPTTNKPELQCSGCHGPGKTLPYLAGSKFHSAPHTEYDRSFHALSKKNGAKS
                     AKCLDCHTRDGDLTSLLPAADPKSPINRANIAETCGKCHGDKSVMQGSGISDRPFLSY
                     QESVHAEAIARGNTSAAVCTDCHNSHDIQPASNSQSSIATVNIPVTCGKCHRTEAGEF
                     IQSVHGLAVARGVSRAPGCTDCHGIHNIKMPSDQMTATATNAVATESCTKCHEGVTLT
                     QEFGVAGARVSTYKDSYHGLASRLGAKTVANCASCHGVHNILPSSDPRSMISAGNLQQ
                     TCGQCHIGAGSNFTKGRIHLDAASASVESVSGSDLGVIGARWVKRIYLPLIFLTIGGM
                     VIHNFLIWRRKALAKRKWENRTVVRLTLNQRVQHWLLLTSFILLVLSGFALQYPDSWL
                     AWVLGANEPLRRALHRIAAVVMLVAGVYHIGYLALTREGRNWVRDMWPRIKDVRDLIQ
                     NLGYYLGVGKARPRIARFGYPEKAEYWAVVWGTLIMGLTGLMIWFKLGFFSFLPRWTI
                     DVALAIHFYEAVLATLAIIVWHFYHVIFDPDVYPINWAFYDGRMSETLYRE"
BASE COUNT          811 a          990 c          999 g          801 t
ORIGIN      
        1 gctttcgggt taactttgat catgcgcgac acctcaacat ggggcgggtc agttgcgcga
       61 tttgccatcg ttcagcaaga ggcggcatag cccaatcgat tccgactggc ttcaacgccc
      121 acgcgatctg tttcggttgc cacacacctc gcgcaaattc gggcgggcgc gatatctcct
      181 cgtgtggcac ctgtcacaag ctgggtggtt acgcacgaac tccgcagtgg accacagcct
      241 ttcggatgaa cttcagtcac gccaaacatg ggccggcgca aaagcttgcg tgcaatgagt
      301 gtcatcaggt acgcccgggc ctgccgcaac gccggcaagt tactactccc gaggcggcca
      361 atcaccatgc ttccggccgc ggtcaaagct gtatgtcttg ccacgacggc aaacgtgcgt
      421 ttggcggcga tgacttttca gtttgtaagc gatgccacac tgggaccaga tggcagtttt
      481 agtaacggag tctgttagct ttcgcgcaaa cttagggttt gcgctacgaa gtgctacgaa
      541 ggaggttagt tatgaaagtc ttttcaatct ttgcggttct gataatcgct gttgccttca
      601 tctcaggctt atcaagtgtg agtttcgatg tctcagcaca ggatgccaag aagcctcaag
      661 atacatacac gctcgccacc gaggcaaaac ttggaccggt cactttcagt cacatcaacc
      721 atacaagcaa gaatcgtaat ttggagggaa cgggacccag ctcgtgtatt gagtgtcacc
      781 acacggccca gccggccact gaagccgcta aacatccgcc gctcaaaacc gcctggcctg
      841 cagatcgtac aaccaccctg agcgcggagg ttctggcgaa ggatccaact gcagtcggcg
      901 cagtctgtcg cgactgccat gcgcgagcgg agacaaagcc gaagttgctg ccggaaaacc
      961 cccaggtgaa gttttctgtg ggaactgaat tagtgacgtt gacgaaccag caggctttcc
     1021 atcgcaattg tgccggttgc cacgacgaaa ttgtgaagac caggacagac gtcgcgccgc
     1081 caacttcaaa gaaatgcact gcttgccata agcgagtggc ggcctgattt ggtgcggcat
     1141 ttgagggcga gtctgcgaaa agttccccac cgcggtcgcg gaggatcgca aaaggctcaa
     1201 tgaaatcgga ccttacccgc cttttcgctc ccggcacggt cattgccaac tagcattctg
     1261 acggggtcac ccccggtttg ccaggttgaa ggatgaaagg tggtaggaat aatgagagtt
     1321 tcgattacgc gatggctcgt attagctagt gcgattttcg cggtgacatt catgattgct
     1381 tcggtgcccg cgcgcacaag ttcgcaggac gataatgccg cgttgtataa atcaaagtgt
     1441 tttgcttgcc atggcgcaac ggctgagaag aagtttgacg ccaccaaggc ggacgatgtg
     1501 ctgattcaga ccgtgcttaa gggaaagaag gcggagaagc cgcccaatat gccggcctac
     1561 gaagaaaaag gcataacggc ggagcaagcg acggctctgg tccagttcat gaagtcacaa
     1621 aagcattagc tcctgtagtt cgtagcgcac aaaggcgctg cgttaattag tggttgctgc
     1681 tcaatcccct gtgtcgcgcg atccgcggca caggagattg ttttgtgctt gtagtagagt
     1741 cttaactcta tggcgcgtgg tcccctactc acggctgtcc tactcgtgct gctgctgtgc
     1801 agccttttcc aatttgccga tcgctcggaa gcacaatcgc caggtccggc agacccagca
     1861 gcgactccgg cggcaacacc gcttcaaccc gctggccaaa ctccgactac aaacaaacca
     1921 gagcttcaat gtagcggctg ccacggcccc ggcaagactc tcccatatct cgctggctcg
     1981 aagttccact ccgctccgca tactgagtac gaccggagct tccatgcact atcgaaaaag
     2041 aacggcgcaa agagtgcgaa gtgtctggat tgccacaccc gagacggcga tttaacttcg
     2101 ctcctgccgg ccgccgatcc gaagtcgccg atcaaccgcg cgaacattgc tgagacttgc
     2161 ggcaagtgcc acggcgacaa gtcggtcatg cagggcagcg gcatcagtga tcggccattc
     2221 ctgtcttatc aagagagtgt tcacgcggaa gctatcgctc gaggaaacac cagcgctgcg
     2281 gtctgcacag actgtcataa cagtcatgac attcagcccg catcaaactc gcagtcatca
     2341 attgccacag tcaacatccc tgtcacttgt gggaagtgtc accggacaga ggccggcgag
     2401 ttcattcaga gtgttcacgg actagccgtg gcccggggcg tgtcgcgtgc ccccggatgc
     2461 acagactgtc acggtattca caacatcaag atgccgtcag atcagatgac agcgacggcg
     2521 acgaatgcag tggcgactga gagctgcacg aaatgtcatg agggcgttac gctaacccag
     2581 gaattcggcg tcgcaggcgc gcgcgtcagc acctataaag acagttatca cggactggct
     2641 tcgcgcctcg gtgcaaagac agttgctaac tgtgccagct gtcacggggt gcacaacatt
     2701 ctgccttcgt ctgacccacg ctcaatgatc agtgccggca atctgcagca aacgtgcggt
     2761 caatgccaca tcggcgctgg ctctaatttc accaagggcc ggattcatct cgacgccgca
     2821 agtgcctcgg ttgagagtgt cagcgggagc gatctgggcg tcatcggagc acgctgggtc
     2881 aaacggatct acctgccact catttttctg actattggcg gcatggtcat tcacaacttt
     2941 ctcatttggc gtcgcaaggc tttggcgaaa cgcaaatggg aaaacaggac ggtggtgcgg
     3001 ctgaccctta accagcgagt gcaacactgg ctcttgctta ctagttttat tttgctcgta
     3061 ctgagcggct ttgctttgca gtatcccgat tcctggctgg cctgggtcct gggtgccaac
     3121 gaacctctgc ggcgcgcgtt acaccgcatt gccgccgtag tgatgctcgt cgctggtgtg
     3181 tatcacatag gctatctcgc attgacacgt gagggtagaa attgggtgcg cgacatgtgg
     3241 ccgcgaataa aggacgtgcg cgacttgatc cagaacctgg gatactatct gggcgtaggc
     3301 aaagcacggc ccagaattgc ccgttttggt tatccggaaa aagctgaata ctgggctgtg
     3361 gtttggggca cgctgataat gggcctgacc ggcctgatga tttggttcaa actcggtttc
     3421 tttagttttc taccgcggtg gacaatagac gttgcccttg ctattcattt ctatgaagca
     3481 gtgctggcta cgctcgcaat aatagtttgg catttctatc atgtaatttt cgatcccgat
     3541 gtttatccca tcaactgggc cttctatgac ggccggatgt ctgaaacgct ctaccgggaa
     3601 g
//
LOCUS       DASUEX010000350         2500 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_285773, whole genome shotgun sequence.
ACCESSION   DASUEX010000350 DASUEX010000000
VERSION     DASUEX010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285773"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            230..1972
                     /locus_tag="VFV61_05860"
     CDS             230..1972
                     /locus_tag="VFV61_05860"
                     /EC_number="2.2.1.2"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional transaldolase/phosoglucose
                     isomerase"
                     /protein_id="HEX4900038.1"
                     /translation="MAAEGMTTSLSQFDAKVAQAISAAQESDLTRRIWRKDAAVWKAE
                     EAHQKIIRNSLGWLTVPGKMTAVADELTAFAEGIRGSGAFNHVMVCGMGGSSLCPEVL
                     RQTFGNQQGYPELLVLDSTDPDVLADFARRIDIERCLFVIASKSGTTTEPLVFYKYWY
                     NQVSKRKANPGDSFIAITDPGTRMVEMATADQFRHIFLNPPDIGGRYSALSYFGLVPA
                     ALMGLDLRRFLARAEAMVRYCGAEAPAAENPGVLLGAIMGECAKAGRNKLTIATDARI
                     AALGLWIEQLVAESTGKEGQGILPVQGEPLGAPDVYGSDRLFVSLGLEEIDERMEQQL
                     AALANAGHPVVQRTLNDNYDLASEFFVWEFATAVAGWRLGINPFDQPNVQESKDATRE
                     LLDYFKSHGQLQDQEPVSGDDTLTVYGSERTGPGLSTASVLDALRAHLATVKSGDYIA
                     LLVYVEETPEIEATLQTIRTRLRDSLRCATTTGYGPRFLHSTGQLHKGGPDSGVFIQI
                     AAADRVDLPIPDEPYTFSVLKQAQALGDFRSLSAHGRRAIRVDLGTDALAGLQRLQKL
                     LADALETSVESSHG"
     gene            1977..>2500
                     /locus_tag="VFV61_05865"
     CDS             1977..>2500
                     /locus_tag="VFV61_05865"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX4900039.1"
                     /translation="MEKLANIGMIGLGVMGQNLALNIERNGFAVAVWDREPAVRERFL
                     ASTRGKQIVDSSSPEEFVRALERPRKIILLVKAGDPVDWTINLIKPFLDSGDIIIDAG
                     NSHFKDTERREQQLKSEGYFMIGSGTSGGEKGALLGPSLMPGGDRDAYEKIRPIWEAI
                     AAKVPDGPCVTYIGP"
BASE COUNT          571 a          648 c          720 g          561 t
ORIGIN      
        1 ccacggcgag gctgccaccg ggacaaaaat cgttctgcac gtccaccacg attagggctc
       61 gtttttcggc ttgagactgc attttgattg attgttcctt tgctctgact ctattacatc
      121 accgctgaaa agttaagcta acccacccaa tgagcccgct tcgcgggctc attgggtgct
      181 cgtgttagac tggcgcattc aaaagaaatc agaaagtgga gagtgatgca tggcggcgga
      241 aggcatgacg acatccctgt cgcagttcga cgcgaaggtt gcgcaagcaa tttccgcagc
      301 gcaggagagc gacttaacgc gacgcatctg gcgcaaagat gctgctgtct ggaaagcgga
      361 agaggcgcat cagaagatca tccgcaactc gctcggctgg ctgacggtcc cgggaaagat
      421 gaccgcggtt gccgacgagt taacggcttt cgccgaagga attcgtggtt cgggggcgtt
      481 caaccacgtg atggtttgcg gcatgggtgg ctcatccctt tgtcccgaag tgttgcgtca
      541 gacctttgga aatcagcagg ggtatcccga actactcgtt ctggattcca ccgatcccga
      601 cgtgttggca gatttcgcac gacgcatcga catcgagcgc tgcttgtttg tcatcgcttc
      661 gaaatccgga actaccactg agccactcgt tttttacaag tattggtaca accaggtaag
      721 caaacgtaaa gctaacccgg gcgattcgtt tattgcgatc accgatcccg gtacgcgcat
      781 ggttgaaatg gcgactgcgg atcagtttcg tcacatcttt ctcaatccac cggatattgg
      841 tggccgctac tcggcgctct catattttgg gctcgtgccc gcagccctca tgggtttgga
      901 cctacggcga ttccttgcac gcgcggaggc catggttcgg tattgcggcg ccgaagctcc
      961 agcagccgag aaccctggag tgttgttggg cgcgatcatg ggcgaatgcg cgaaggccgg
     1021 gcgtaacaaa ctaacaatcg caactgatgc caggatcgcc gcactcggtt tgtggattga
     1081 gcagctagtc gcagagagca ccggaaagga aggacaggga atactgccgg tgcaaggcga
     1141 accgctggga gctccggatg tctatggatc agatcgcttg tttgtttcgc taggccttga
     1201 agaaatcgat gagcgcatgg agcaacagct tgctgcgctg gctaacgccg gtcatccagt
     1261 tgtccagcgt accctgaacg acaattatga cctcgcttcg gagtttttcg tttgggagtt
     1321 tgctacggcc gtcgctggct ggcgcctggg aattaatccg ttcgatcagc caaacgtcca
     1381 ggagtcgaaa gatgcgacca gggaactcct cgattacttc aaatcccatg gacagctaca
     1441 agatcaggag ccagtgagtg gcgacgacac cttgacagtt tacggttctg aacgaaccgg
     1501 acccggatta tcgacggctt ctgtacttga tgccttgcga gcacatctgg caaccgtcaa
     1561 atctggcgac tacattgcac ttcttgttta cgtcgaagag actcccgaaa tcgaagcgac
     1621 gctgcaaaca attcgcacaa gattaaggga ttcgctgcgc tgtgctacca cgaccggcta
     1681 cggcccacgt ttcctgcatt cgacagggca actgcataag ggtggtcccg atagcggcgt
     1741 gttcatccaa atcgccgctg ccgatcgcgt tgatctgccg attcccgatg aaccttatac
     1801 cttcagcgtg ttaaagcagg cccaggcatt aggcgacttt cgttcgctgt cagcgcacgg
     1861 acgacgagcg attcgcgtag acctgggaac agacgcactt gccggcttac agcggctgca
     1921 gaagttgttg gcggacgcat tggaaacttc ggttgaatca agtcacggtt aagtagatgg
     1981 aaaaattagc aaacattgga atgattgggc tcggagtgat gggtcaaaac ctggcgctga
     2041 atatcgaacg caatggtttc gcagtcgccg tttgggatcg ggagccggca gtgcgtgagc
     2101 gttttctggc aagcacaaga ggaaagcaga ttgttgactc gagttctcct gaagagtttg
     2161 tgcgtgctct ggagagaccg cgcaaaatta ttctcctggt aaaggcaggc gacccggtcg
     2221 attggacgat caacctcatc aagccttttc tcgacagcgg cgacatcatc atcgacgccg
     2281 gcaattcaca cttcaaagat acggagcgac gtgagcagca attgaagagc gagggttatt
     2341 tcatgatcgg ttccggaacc tccggcggtg aaaaaggcgc gttgctgggg ccaagtctaa
     2401 tgccgggcgg cgatcgcgat gcttatgaaa agattcgccc gatttgggaa gcgattgcgg
     2461 caaaagtccc cgatggtcct tgcgtgacat acatcggacc
//
LOCUS       DASUEX010000351         2244 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_508253, whole genome shotgun sequence.
ACCESSION   DASUEX010000351 DASUEX010000000
VERSION     DASUEX010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508253"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1029)
                     /locus_tag="VFV61_05870"
     CDS             complement(<1..1029)
                     /locus_tag="VFV61_05870"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX4900040.1"
                     /translation="MSLAALLAAAGSAGLAQTPTPTPRPQQREATRPPGTETQNPNLP
                     PGTQPGAPTAAPGTQQPPPTAPPGNPNAPTSQPTPPTPTNPTEMVTPDVTTPAETTPL
                     QEPREPVFPVVERRPLPPMPNLTRLGVTSDNTLTLSLNDAVRKALQNNNDIEIARDDV
                     RFAETQLRSLEGVYDPIFSLTPQIDKRISPQQNRFAGATTSGTNSNTTYSINPAIFKQ
                     FSMGGGSYDLTFTNSKTKSNSTANLLNPFYSGNLGLTFTQPLLRNRSIDNNRRQIRIQ
                     KKRLEQSDADFRLRTIDVISRVQQAYWELVFALRDQDNQLDNLNLSRENLRNVEAQIS
                     VGAKAPLER"
     gene            1393..>2244
                     /gene="gap"
                     /locus_tag="VFV61_05875"
     CDS             1393..>2244
                     /gene="gap"
                     /locus_tag="VFV61_05875"
                     /inference="COORDINATES: protein motif:HMM:TIGR01534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEX4900041.1"
                     /translation="MAVKVGINGFGRIGRNILRTSLGDPDIDFVAVNDLTDTKTLAHL
                     LKYDSVLGNLDHEITSTENGIRVDQDEFRVFSQRDPAAIDWDSVGAEIVIESTGIFTK
                     AEDAKKHLRGSVKKVIISAPAKNEDITIVLGVNEGQYDAAAHHIISNASCTTNCLAPV
                     AKVINEKFGIRSAQMTTIHSYTNDQQLLDLPHKDLRRARAAALSMIPTSTGAAKAVAL
                     VLPELKGKFDGISVRVPTPNVSLVDVVIEVEKDTTAEEVNRALKDSANEELQGILAFS
                     EEPLVSSD"
BASE COUNT          505 a          577 c          657 g          505 t
ORIGIN      
        1 gcgctccagc ggcgccttcg caccaacaga gatttgcgcc tccacgttgc gtaagttttc
       61 ccgtgacaga ttcaggttgt ccagttggtt atcttgatct cgcagagcga acactaattc
      121 ccagtaggcc tgctgtacac gcgaaatcac atcgatcgtg cgcaggcgaa agtctgcgtc
      181 agactgctcc agccgtttct tctgaatgcg aatctgacgc cgattattat cgatagagcg
      241 attgcgtaag agcggctggg tgaaagtgag accgaggttg ccggagtaga acggattcaa
      301 caggttcgct gttgaattag acttggtctt gctgttggtg aacgtcagat cataactgcc
      361 gccgcccatg gaaaactgtt tgaagatagc cgggttgatc gaataggtcg tatttgaatt
      421 ggtacctgat gtagttgccc cagcaaagcg gttctgctgc ggtgagattc gtttgtcgat
      481 ctgtggcgta agcgaaaaaa ttgggtcgta gacgccctcg agcgaacgca gttgagtttc
      541 ggcaaagcgc acatcatcgc gcgcaatctc gatgtcgttg ttgttctgca gcgctttacg
      601 tacggcatcg ttgagcgaaa gcgtcagcgt gttgtcgctt gtcacaccta atcgggtcag
      661 attaggcatc ggcggcaacg gtcttctttc gacaacggga aagactggtt cacgtggttc
      721 ctggagcgga gtggtctcgg ccggcgtcgt gacgtctggc gtcaccattt ccgtcggatt
      781 cgtcggcgtc ggcggcgtcg gctgggacgt gggcgcattg ggattacccg gcggtgcggt
      841 cgggggcggt tgctgcgttc ctggcgcggc ggtcggcgca cctggttgcg tgcccggagg
      901 caaattggga ttctgcgtct cggtacctgg tgggcgggtc gcctcgcgct gctgcggccg
      961 cggcgtcgga gtcggagttt gcgccaagcc ggcgctaccc gcagccgcga gcaatgcggc
     1021 taaagacatc actcgcacta attctatagg cttaatagta agcatcagac caaaagctcc
     1081 ttaactctct acaaagattt cgctctcgag tgtcagacaa actacaagtg agcagacacc
     1141 accggatcag gtttggttac gttaacaaaa cggtgcaggt ttcataaagt tgaacaataa
     1201 gctcacttta cggccgctaa ccgctgcgaa acagtggctt gacactacaa gccgcgccgc
     1261 ataatctgag aactctttta ccagtaagtt ccatctaaca tgctcgtcct tgtggaagtg
     1321 tgcgctcagg gtcacagcgc agtgagagtt gtgcagagat tgtctcaata acgtgaggag
     1381 gactttaacg aaatggcagt aaaggttggc attaacggtt ttggtcgcat cggtcgaaac
     1441 atcctgcgca cttcattggg cgatcccgat atcgatttcg tggccgtaaa cgatttgacg
     1501 gacacgaaga cgctcgcgca cctgctcaag tacgactccg tactcgggaa tctggaccat
     1561 gaaatcactt ccacggaaaa cgggatcagg gtcgatcagg atgagtttcg cgtcttctcg
     1621 caaagagatc ccgcggcgat tgattgggac tcagtcggcg ctgagatcgt gattgaatcg
     1681 acgggaatct tcaccaaagc cgaggatgcc aaaaaacatc tgcgtggatc ggtgaagaag
     1741 gtgatcatat cggcgccagc caagaacgag gacatcacca tcgtgctcgg cgttaatgag
     1801 ggtcaatacg acgccgcggc gcaccacatc atctctaacg cctcctgtac cactaactgc
     1861 ctggcgccgg tagccaaggt gatcaacgaa aagtttggta ttcgctcagc gcagatgacg
     1921 acaattcact cctacaccaa tgaccaacaa ctgttggatc tgccgcacaa agatctgaga
     1981 cgagcgcggg cggcggcgct ttcgatgatt ccaacctcca cgggcgccgc caaggcggtc
     2041 gctttggtgc tgcccgagct caaaggaaag ttcgacggca tctcggtgcg tgtgccgacg
     2101 cccaacgtgt cgcttgttga cgtcgtgatt gaagtggaga aggacacgac tgccgaagag
     2161 gtgaaccgtg cgctgaaaga ctcggctaac gaagagctgc agggaatcct ggctttctcg
     2221 gaagagccat tggtttccag cgac
//
LOCUS       DASUEX010000352         2791 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1309463, whole genome shotgun sequence.
ACCESSION   DASUEX010000352 DASUEX010000000
VERSION     DASUEX010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2791
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1309463"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="VFV61_05880"
     CDS             <1..270
                     /locus_tag="VFV61_05880"
                     /inference="COORDINATES: protein motif:HMM:NF014871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX4900042.1"
                     /translation="GSRVVDVSVNGVPLDDQKKYSLATTNFLAEGGDGYDVLKTARVL
                     IAPAQGQTDFDVVRQSLATGPITPKVDGRIKRLDNTRRKQADCPE"
     gene            275..787
                     /locus_tag="VFV61_05885"
     CDS             275..787
                     /locus_tag="VFV61_05885"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX4900043.1"
                     /translation="MIADDIYEDDTSPLAKYEAKELFQEAYEAQLAQNFETAIELYRR
                     SIEMYPTAEAHTFLGWVYSFQNRYDDAISECLEAIRVDETFGNPYNDIGSYLLAQGDS
                     YGCVRWFKRALLAPRYESYAFPHFNLARVYEKRHRYLDAVRHYSLALEQQPGFAEANS
                     ALRRLQALLN"
     gene            complement(867..1259)
                     /locus_tag="VFV61_05890"
     CDS             complement(867..1259)
                     /locus_tag="VFV61_05890"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HEX4900044.1"
                     /translation="MATMNSYTDPAIRITLLPRDTNSAGTIFGGVILSYIDMAGAIEA
                     HRRTRMDRFVTVAMREVIFHKPVFVGDLVSFYSETLRVGTTSITVRVVVEAERVGSSS
                     ERVRVTEAEVVYVAVDANRQKTKINLPQ"
     gene            1562..1786
                     /locus_tag="VFV61_05895"
     CDS             1562..1786
                     /locus_tag="VFV61_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900045.1"
                     /translation="MVIVASLVYVLSIFEIVVSDADALGEMNDLGTLMLGGFILAVVG
                     AIALTIIRLRIREKRPESAQFISINSREED"
     gene            complement(1788..2435)
                     /locus_tag="VFV61_05900"
     CDS             complement(1788..2435)
                     /locus_tag="VFV61_05900"
                     /inference="COORDINATES: protein motif:HMM:NF042734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX4900046.1"
                     /translation="MYPAGNLFIPIAHGRLEAILKEPADSARGVALVLHPHPLGGGTM
                     HNKVVFRAAAALNEMGLTTLRINFRGVGQSSGTHDEGRGEVEDVRAGLTYLEENYPYL
                     PLTLCGFSFGARVGLEVGIADERVMNLISIGTPLGKYDFSFLENCRKPILFVHGAEDE
                     FGAATEVERLARQLEAVTDVSVKIIAGADHFFAGHLDELKLAITEWMTKQLANAG"
     gene            2606..>2791
                     /locus_tag="VFV61_05905"
     CDS             2606..>2791
                     /locus_tag="VFV61_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900047.1"
                     /translation="MQLHIPSVRGVNFLSPDLQSECLPATSGDTRPQSFAKELSLKKG
                     KKRRALVVDDIADVTEML"
BASE COUNT          662 a          739 c          740 g          650 t
ORIGIN      
        1 ggttcacgag ttgtggacgt tagtgtcaac ggagttcctc tggacgatca gaaaaagtac
       61 agcctggcca cgacaaactt cctggcggag ggcggcgacg gctacgatgt tctgaagacg
      121 gcgcgtgttt tgatcgcgcc ggcacagggt cagaccgact ttgacgtggt gcgtcaatcg
      181 ctggccaccg gcccgatcac gccgaaggta gacggtcgca tcaaacggtt ggataatacg
      241 cgcagaaagc aggcggattg cccggagtaa gctgatgatt gctgacgata tttacgaaga
      301 cgatacttcg ccgttggcaa agtacgaagc gaaggaatta tttcaggagg cttacgaagc
      361 gcagttggcg cagaactttg aaactgccat cgagctctac cggcgctcaa tcgagatgta
      421 cccaacggca gaggcccaca cttttctcgg gtgggtctac agtttccaaa atcgctacga
      481 cgacgccatt tctgaatgcc tggaagcaat tcgtgtcgac gaaacgttcg gcaacccata
      541 caacgatatc ggtagttatc tgctggctca gggagactcg tacggatgcg ttcgctggtt
      601 caagcgtgcg ctactggcgc cgcgttatga atcttacgcg ttcccgcatt tcaatctggc
      661 gcgagtttat gagaaacgac atcgctacct cgatgcagtg cgccattact cactcgcact
      721 cgaacaacaa cccggcttcg ctgaagctaa ttccgcacta cgtcggctcc aggcgctctt
      781 gaattgaggc ggttggagag accctcgcta gtgataactg tgatgatttg cgaatccgcg
      841 atccacacgg cagaagtaca attttctcat tgaggaaggt tgatttttgt tttttgccgg
      901 ttcgcgtcga ctgcgacata aacaacttcg gcttcggtaa cgcgcactcg ttcgctgctt
      961 gatcctactc gctccgcttc aactactact cgcaccgtaa ttgacgtcgt gcctactcgc
     1021 agcgtctcgc tatagaaact cacaaggtcg ccaacaaata ccggtttgtg aaatatcact
     1081 tcgcgcatgg cgacggtcac aaagcggtcc atacgtgtgc gtcgatgcgc ttcgatcgcc
     1141 ccggccatgt cgatatagct caggatgaca ccgccgaaaa tcgtgcccgc cgaatttgta
     1201 tcgcgtggca gaagagttat ccggattgcg ggatcggtat aggagttcat ggtagccatc
     1261 ctaaattgcg aaatcagcag cgggaagcgg cttcaccgcg cccacgggcc tcagattcaa
     1321 ccacttaacc gcaaagacgc caagtcgcag agaagcgtta gcagcggcat tgagtggttc
     1381 cgactcgaat tcgtaagtgc caaaggtgag ctggaataaa ctactgaact gcaaagaaca
     1441 ttcttcgcga cacagcgact tggcgtttag gttttttctt tgggaacaag ttcacgccaa
     1501 cgtgtgtcta acgaagtatt gctggccgcg tctcactgat ctacaaggaa aggttattca
     1561 aatggtgatc gtggcttcgt tagtttacgt cttgagtatt ttcgagatcg tcgtttctga
     1621 tgcggacgct ttaggggaaa tgaatgatct cggaaccttg atgctggggg gattcattct
     1681 cgccgtagtt ggagcgatag cgctgacgat aattcgactc agaattcgcg agaaaaggcc
     1741 ggaatcggcc cagttcattt ctatcaattc gcgggaagaa gactgagtta tccggcgttc
     1801 gcgagctgtt tagtcatcca ttccgtaatt gccagtttca gctcgtccag gtggcccgcg
     1861 aaaaagtgat ccgccccggc aatgatcttt acgctgacgt cagtaaccgc ttccagttga
     1921 cgtgccaacc gctctacttc agtcgccgcg ccaaactcat cctctgcgcc gtggacaaag
     1981 agaatcggtt tgcggcaatt ttctaaaaaa ctgaaatcgt atttacccag cggtgtgcca
     2041 atgctgatga gattcataac gcgctcgtcg gcaattccga cctcgaggcc gacgcgcgcg
     2101 ccgaaagaaa aaccacacaa cgttaatggc aagtacgggt agttttcttc gaggtatgtt
     2161 aagccggcgc gcacgtcctc gacttcgccg cgaccttcat catgtgtccc ggaactttgt
     2221 cctaccccac gaaaattgat acgcagcgtc gtcagcccca tctcgttcag cgccgcggca
     2281 gcgcggaaga cgaccttgtt gtgcatcgtg ccgccgccaa gtggatgcgg atggagcact
     2341 aacgcaaccc cgcgcgccga atcagcaggc tctttgagta tggcttccag acgtccatga
     2401 gcgatgggaa taaacagatt tccggcggga tacattgcga ttcaacctac caaatcgaac
     2461 cttgcgtgtc cacgacttgc aatcggcaag gtgccatttt tcgttaccgt taaaagtgta
     2521 gacaccggtg aactatttcg ctaaattgtt tgcagtcttc tgatcctcag gggacaacgg
     2581 tggcgcgcag aaaaaggtcg aacacatgca gttacatatc ccttcggttc gtggcgtcaa
     2641 tttcctttcc ccagatctgc aaagtgaatg cttacccgcg acgtcaggcg acacccggcc
     2701 gcagtccttc gcgaaggagc tttcgctgaa aaaaggaaaa aagcgacgag ccttggtggt
     2761 tgacgacatc gcggatgtca ccgagatgct c
//
LOCUS       DASUEX010000353         3122 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1194866, whole genome shotgun sequence.
ACCESSION   DASUEX010000353 DASUEX010000000
VERSION     DASUEX010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3122
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1194866"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..576)
                     /locus_tag="VFV61_05910"
     CDS             complement(<1..576)
                     /locus_tag="VFV61_05910"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX4900048.1"
                     /translation="MAEFIVRLGTPAGEVVTRTVDAPGVNEARARLEREGFRVFNVTP
                     PRTSGVTTLTRLGGRGAHARVKPNDFLLFNQQLAALFRAGIPILQAITMLRKRAASAR
                     LRAVLGDVEEQIRGGAALSAAFASQGSLFPRIYTASILAGERSGALDEVLGRYVTYMR
                     RNVALRRKIRGALAYPMFLLFASLCMVTFLTV"
     gene            1017..2045
                     /locus_tag="VFV61_05915"
     CDS             1017..2045
                     /locus_tag="VFV61_05915"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX4900049.1"
                     /translation="MHLPAKFAVRIAFLSCCVLACATSATIHAQETAQPKPTVEPTST
                     PAAAKPDEQRKTGVDLDGPVIVNTDLITLTVTVTDTYGRYVSGLTKNAFTLLDQKTPQ
                     EITFFSDDDSPVSVGVIFDVSGSMSGDKVKKAREALSKFIQTSHNSDEYFLIGFNSRA
                     QLLLDKTRDGNAVLDKLMFVETKNNTALYDACYLGVEKVQRGAHPKRALLLISDGQDN
                     NSRYTFNELRRLLKESDVVLYGIGILSGGDAGSQLGMEGQGILDELSNVSGGKAFFPR
                     SAVEMDDIFEQIALELRHQYSIGYKPNNFVNDGKWHKIKVKVTPPRGLPKLFVRSKEG
                     YFAVVGAR"
     gene            2575..>3122
                     /locus_tag="VFV61_05920"
     CDS             2575..>3122
                     /locus_tag="VFV61_05920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900050.1"
                     /translation="MTKQRIIHSDERTLRVFLLLGIIAILLAGLAGSSQVPTGRAQET
                     RLRRSGQTTTARPTATPTPKSAPVTTRPTQTPAAQTPAPVATPPATVTQTPASQPTAT
                     PTPQVIQTGVDKIAPELGPPPPIPKLKPKPTPTPPEEFGEGEVVRVDTELVTLNVRVI
                     DRNNRPIDNIQENEIHVYEDTV"
BASE COUNT          736 a          924 c          828 g          634 t
ORIGIN      
        1 gactgttaga aatgtgacca tgcagagcga cgcaaagagc aggaacatcg gataagcgag
       61 cgcgccgcga atcttgcgtc gcaacgccac attacgccgc atgtaagtca catagcgccc
      121 caagacttca tcgagcgcac cggagcgctc gcccgccaga atcgatgccg tgtagatgcg
      181 tggaaagaga ctgccttgcg aagcaaaggc agcggataga gctgcgccgc cgcgaatctg
      241 ttcttcaaca tcaccgagca ccgcacgaag gcgcgccgaa gccgcgcgct tacgcagcat
      301 tgtgatggct tgcaggatgg gaatgccggc acggaagagg gccgcgagct gttgattaaa
      361 aagcaggaag tcgtttggct taacgcgcgc gtgtgctccg cgaccaccga gccgcgttaa
      421 ggtcgtgact ccgctggtac gcggcggggt tacgttgaaa acgcgaaagc cttcgcgctc
      481 gagccgcgcg cgtgcctcat ttacgcccgg cgcatctaca gtgcgcgtca ccacctcgcc
      541 cgcaggcgtc cctaatcgaa caataaactc agccatacta aagaaaacca gctctgattg
      601 tgggactacc ccttgaaatg aagcgaaagt atagcacgcg ctaactcagc cctgaggaat
      661 cgccaggcct gcctcgaatt gcacgcccca accggccgga agttgcagcg gtctcccaat
      721 ccaagttgcc gacccaagct cgctttcgtt gctccggcat caacgccagt tccgtctcag
      781 ttacccttct cggtttactt cttgcgcggc ctcacagccg tgtcctacaa tgccgtcgtc
      841 ccaaattgaa atcggaatag cgcgcgctcg gtctcccacg caagccgttt ccagcggtcc
      901 cttacaaaga aaaagagttt ccctgggcat caccggttct ccggaaagca tctgtgttag
      961 cagccctttc gaccgaacga tcgttccaga ctctgaagca gacaagcggg ggtcaaatgc
     1021 acttaccggc taaattcgct gtcaggattg ccttcctgtc atgctgcgtt ctggcttgcg
     1081 ccaccagcgc cactattcac gctcaggaaa ccgcccaacc gaagccaact gtcgaaccaa
     1141 caagcacccc tgccgcagcc aaaccagacg agcaacgcaa gaccggtgta gacctcgacg
     1201 gccccgtgat cgtcaacacc gacctgatca cgctgaccgt taccgtaacc gacacctacg
     1261 gacgttatgt ttccggctta actaaaaacg catttaccct cctcgatcaa aaaacaccac
     1321 aggaaattac ttttttcagc gacgatgatt cgccggtttc cgtgggggtg atttttgatg
     1381 tctcgggctc aatgagcggc gacaaagtaa aaaaggctcg cgaagcgctt tcgaagttta
     1441 ttcaaaccag tcacaactcc gatgagtatt ttttgattgg cttcaactcg cgcgcgcaac
     1501 ttctgctcga caagacccgc gacggcaatg ctgtgctcga taaactgatg ttcgtggaga
     1561 ccaagaacaa caccgcgctt tacgatgctt gttatctggg tgtcgagaag gtacagcgcg
     1621 gcgctcatcc gaaacgggcc ctgttgttga ttagcgacgg gcaggacaat aactcgcgct
     1681 acaccttcaa cgaactaagg cgtctcttga aagagtccga tgtcgtgctt tatggaattg
     1741 gcatccttag cggcggggac gccggcagcc agctgggcat ggagggccaa ggcattctgg
     1801 atgagttgtc gaacgtttcc gggggcaagg ctttcttccc gcgctcggca gtggagatgg
     1861 atgacatctt tgaacaaatc gccctcgagc tgcgccatca atactcgatt ggttacaagc
     1921 ccaacaactt cgtcaacgac ggcaagtggc acaagatcaa ggtcaaggta actccgccgc
     1981 gcggtctgcc taaattgttc gtgcgttcca aggaaggcta tttcgcagtc gtcggggcgc
     2041 gctagcacaa attgccgatt ggcgattgtg gattgccgaa tgaacaatac caaccgccag
     2101 gacgcttggg acgcagaatt cgtcaagagc ttccgttacg aattgggttt agttttccgt
     2161 cctggcgtac ttggcgtttc cttgggcttc ggcggtttta cttaatcggc aattggcaat
     2221 cggcaattga aagtctccgg caactaaccc cctctcccaa acatctttct accgtgtgcg
     2281 cagtggacgc cggcgatctg ttgcccgagc ccggcgaaat cagcggatta aattcggcgc
     2341 tccggcccga tcttcagggc ttacggagcg cttcgcatga gtagaggtaa agagagcgtg
     2401 atctcattct caccgggctt cagcccggtg ttgatcgtgg ctcggacggg aaaccgttta
     2461 aacggtttcc atttggcagc tgggtgcgag caccgcgcta aagcgcgtta atgagacccg
     2521 caccaaacga aggtttgcgt tacaatcttt cgcgaagaga gggctaacag gtcggtgaca
     2581 aagcagcgca tcattcattc agacgagcgc accttgcgcg tgttcttact gcttggcatt
     2641 attgccattc ttttggcggg actcgccggg tcgagccagg ttccaaccgg ccgagcgcaa
     2701 gaaacgcgtt tgcgacgcag cggacaaact acgacggccc gtcccactgc tactccaacg
     2761 ccgaaatctg caccggtaac caccaggcca actcagactc cggcagctca aacgcccgcg
     2821 cccgtggcta cacctcccgc aaccgtcacc caaacaccgg caagtcaacc gaccgccaca
     2881 ccgacaccgc aagttatcca gactggagtc gacaagatcg cgcctgaact tggacccccg
     2941 ccaccgattc caaagttgaa accaaagccc acaccaactc cgccggagga gtttggcgaa
     3001 ggcgaagtgg tgcgtgttga taccgagttg gtcacgctaa atgtgcgcgt tatcgatcgt
     3061 aacaatcgcc ctatcgataa cattcaggag aatgagatac acgtttacga ggacaccgtg
     3121 ga
//
LOCUS       DASUEX010000354         2958 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1561776, whole genome shotgun sequence.
ACCESSION   DASUEX010000354 DASUEX010000000
VERSION     DASUEX010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2958
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1561776"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            150..623
                     /locus_tag="VFV61_05925"
     CDS             150..623
                     /locus_tag="VFV61_05925"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX4900051.1"
                     /translation="MSEAERASLGAAAPDFTLPDGEGGSWTLSAQKGKVVVLLFYPGD
                     ETPICTRQMCSVRDRWDDYMATGAEVVGISTDSVDSHQKFANHHRLPLRLLSDADQKV
                     ANLYGARSLVPGKVARSVFVIDGNGIIKHRKVQPLGLFRPKDDEILGAIRAAQDN"
     gene            727..1011
                     /locus_tag="VFV61_05930"
     CDS             727..1011
                     /locus_tag="VFV61_05930"
                     /inference="COORDINATES: protein motif:HMM:NF003817.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HEX4900052.1"
                     /translation="MAETIKCKHCGEKRPALGFAPFPNELGQRLATEICQPCWAGWLQ
                     KQTQIINHYGLDLTNPDAQNFLFDNIKGYLFSEVQELPQIDTSKEGSVKW"
     gene            1293..>2958
                     /locus_tag="VFV61_05935"
     CDS             1293..>2958
                     /locus_tag="VFV61_05935"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX4900053.1"
                     /translation="MPRPTSRIALALIASLSLGALPTHFSQAQRNAIDTYAITNARIV
                     PVSGPPIERGTVVMRDGLITAVGANVSPPADARVIDGNGLTVYPGLFDSNTSLGMPQP
                     SPSPSPTTTPAGGGFGGQRPQAAMLTALNSSQPAGLQPEVLAEDFIKPGGDQIEAARQ
                     MGITTALSAPRQGIWMGQSALINLAGDTTQEMIVRSPVALHVDFTPLRSGVYPNSLMG
                     VFAVLRQMMLDANRYREAQQAYARSPRGMKRPDQDRALAALLPVLDGSLPVVMYADSE
                     REINRAVDLADEFKLRAIIAGGVEAGKVADRLRERKVPVLLSLNFPRRTTAAIPEADP
                     EPLRVLRERVEALKTAGRLAAAKVKFAFQSGAMTNTADFYPNIVRAIENGLAREEALR
                     ALTISAAEILGVNDRLGSIEAGKIANLTVTRGELFTRNPTFAYVFIDGRPVDLKPATP
                     AAADTASSTAATGNWTLTVNTGSADLAVTLVLQQEGERLRGSIQGALGSGEISNASFG
                     PGGDFRFTVPVTLEGQTTEATFTGKLTSNEMQGSVTFVGRAPGTFSG"
BASE COUNT          646 a          823 c          860 g          629 t
ORIGIN      
        1 tcaggagcat cgctcgagta attgtcagtt aatattgctt ggcgaacttg gcgtgtcttg
       61 gcgacttggc ggtgaatcgg tggttatgaa cttctggtac tctcgtgtaa atctttgacg
      121 gctcatgtat cctaaggtca gagagaataa tgagcgaagc tgagcgcgca tcactgggag
      181 ccgcggcgcc ggacttcact ttgccggacg gcgaaggagg gtcctggacg ttgtcggctc
      241 aaaaaggcaa ggtggttgtc ttacttttct acccgggcga cgagacacca atttgcacgc
      301 gccagatgtg ttccgtgcgc gatcgctggg acgactatat ggcgaccggg gctgaagtgg
      361 tgggcatctc gaccgattcg gttgactcgc atcaaaagtt tgccaaccac catcgactgc
      421 ctctgcgact gttgtcggac gcggatcaaa aggtcgcgaa tttgtatggg gcgcgttcgc
      481 ttgtgcccgg caaagtggcg cgttccgttt tcgtgatcga cggaaacgga atcattaagc
      541 atcgcaaggt gcagccgctg ggattgtttc gcccgaaaga cgatgagatt ctgggtgcaa
      601 tccgggcagc gcaggataac taagtctcag gtctcaagtc tcaagtcggg agcctcgctt
      661 aacctgaaac ctgagacctg agacctaaga cttgagacct gagacttgag actttcgcgg
      721 agcgttatgg cagaaacaat taagtgcaaa cattgcggag agaagcgacc agcattgggc
      781 ttcgcgccgt ttccgaatga gttgggacag cgcctggcaa cagagatttg ccaaccctgc
      841 tgggccggtt ggctccaaaa acaaacgcag atcatcaatc actatggtct ggatctgacc
      901 aatccggatg cgcagaactt cctcttcgat aacataaagg gttatctatt tagcgaggtg
      961 caggaactac cgcagatcga taccagcaag gaagggtctg tgaagtggta agcaacgtcg
     1021 aacgtccaat gtccaaagtc caaagtccag gtttagaatt cttgcctcaa cttgagaatg
     1081 tcgggttcta tgaccgattg cggcacagca gcagggcccg tgacacctcg aagctttgga
     1141 cattggacgt tggactttga acgttggact ttggacgtta ggcccttttt cgcgctaagc
     1201 tctcgccact tcgtttctga gaatgtgttt gccctggtct ccccaggccg gcgcacgaac
     1261 cccaaaacca ctgcatgcat acccgatcgt ctatgccaag accaacgtcg cggattgccc
     1321 tggcgctgat cgcttcgctg tcgctgggag cccttcccac tcacttctct caggcacaac
     1381 gcaacgccat cgatacctac gccattacca atgcgcgcat cgtccccgtt tccgggccgc
     1441 ccattgaacg cggcacggtc gtgatgcgcg acggattaat tacagcggtt ggagcaaacg
     1501 tttcgccgcc cgccgacgcg cgcgtaatcg atggcaacgg attgactgtc tatcccggtc
     1561 tcttcgattc caatacgtcg ttgggaatgc cgcagccttc gccttcacct tcaccaacga
     1621 caacgcccgc tggcggaggg ttcggcggac agcgacccca agctgccatg ctgacggcat
     1681 tgaactcttc gcaaccggcc ggcttacaac cggaagtact ggccgaggac ttcatcaaac
     1741 cgggcggcga tcagatcgaa gcggcgcggc agatgggaat cacgactgca ttgtcagcgc
     1801 cgcgacaggg tatctggatg ggacagtcag ccctgatcaa tctcgcaggt gataccacac
     1861 aggaaatgat cgtgcgttca ccggtagcgt tgcacgtgga cttcactccg ttgcgcagcg
     1921 gcgtctaccc caactctctg atgggagtct ttgccgtgct gcgccagatg atgctcgacg
     1981 cgaatcgcta tcgagaggcg cagcaggctt acgcgcggtc gccacgcgga atgaagcgcc
     2041 cggatcagga ccgggcctta gcagctttac tacctgtgct cgatggatct ttgccggtgg
     2101 tcatgtacgc cgacagcgaa cgagagataa accgggcggt tgatctcgcc gacgaattca
     2161 agctgcgggc aataattgcg ggcggagtcg aagcggggaa agttgccgac cgtctgcgtg
     2221 aacgcaaggt gccggtcctg ctttcactaa actttccgcg ccgcacaacg gctgcgatcc
     2281 cggaggcaga tcctgagcca ctcagggtct tgcgcgagcg cgtggaagcc ctgaagacag
     2341 ccggacgact agcggccgcg aaggtgaagt ttgctttcca atcgggagca atgacgaata
     2401 ctgccgactt ctatccaaac atcgtccgtg ccatcgagaa cggtctggca cgtgaagaag
     2461 cattacgggc actcacgatt tcggcggccg agattctcgg cgtaaatgac cggctcggct
     2521 cgatcgaagc cggcaagatc gcgaatctga ctgttacgcg cggagaactc ttcacgcgca
     2581 atcccacgtt cgcctacgtc ttcattgatg gtcgtcccgt tgatcttaaa cccgcaactc
     2641 cggccgctgc ggacacagct tcaagcacgg ctgccaccgg taattggacg ttgactgtga
     2701 acaccgggtc cgctgactta gctgtaactc tggtattgca acaagagggt gagcgactgc
     2761 gcggctcgat ccagggggct ctcggtagcg gtgagatctc aaatgcgtct tttggtccgg
     2821 gtggtgattt taggtttacg gttccggtaa ccctggaagg ccagactacg gaagcaactt
     2881 tcacgggcaa gctcaccagc aacgagatgc agggcagcgt caccttcgtc ggccgcgcgc
     2941 cgggaacatt cagcggca
//
LOCUS       DASUEX010000355         5825 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_393278, whole genome shotgun sequence.
ACCESSION   DASUEX010000355 DASUEX010000000
VERSION     DASUEX010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5825
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393278"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..708
                     /locus_tag="VFV61_05940"
     CDS             <1..708
                     /locus_tag="VFV61_05940"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX4900054.1"
                     /translation="GHKWFISGALGSAFAIVMAVTDPNAEAHARASMILVPTDTPGFN
                     IIRAVPVMGSGGVGGHCEIRFEDCRVPLSNLLGDRGKGFKLAQARLGPGRIQHCMRWI
                     GAAQRSFEMMCEYALERESFGEPLANKQTVQNWIADSAAEIEAARLLTLKAAWKLDRE
                     EDARIEISVIKFFAARVLHDVIDRAIQVHGALGYSKDTPLEMFYRDARAARIYDGPDE
                     VHRQAVAQRVLKAFKRE"
     gene            839..1927
                     /locus_tag="VFV61_05945"
     CDS             839..1927
                     /locus_tag="VFV61_05945"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEX4900055.1"
                     /translation="MTFIKDSIALRPGEELDLETLDHYLRRQLSTLTFDPGSALELGQ
                     FPGGHSNLTYLVRYGEKEFVLRRPPIGPVAPTAHDMPREYNLLCVIHPHFPLAPKPFL
                     LCEDVAVIGAPFYLMERRQGLIVRQSLPGPLSDDLIARRQLSETVVDTLAALHAVDIY
                     ATGIVSIGKPEGLVRRQVHGWTERWRRSKTGDLDQMERVIEWLEQRMPPEADGTAATI
                     VHNDFKLDNLMLAGDDPTSVIAVLDWEMTTVGDPLIDLGLLLTYWTMRGAKDSEQNRS
                     LTAVTNGPGWFTREEILARYQAKTGRDLSRIVFYEVFARFKIAVVIQQIYFRYVKGQT
                     QDQRFRTLDVLVNALACEALTLAEGRKA"
     gene            2161..2910
                     /locus_tag="VFV61_05950"
     CDS             2161..2910
                     /locus_tag="VFV61_05950"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEX4900056.1"
                     /translation="MPDLQFHTLRFDSLPSTNLEAARRAQEGAPEGLCIVASEQTAGR
                     GRLGRQWVSPRNAGIYCSTLLRPQFDPSSWPLITLMAAIVVNEALLETCALTTDIKWP
                     NDILYEEKKLCGILAETVETSTGRALVVGIGINLTNDSFPPELAATATSVSVATGSPP
                     NLEDVLSKLQQCFVVWYHRLNQPTGAGDIIRAWSDHSSYADGKKIMIRDADTEFCGTT
                     RGITVDGALKVESETGEIKIFRAGDVSLRSG"
     gene            2963..4285
                     /locus_tag="VFV61_05955"
     CDS             2963..4285
                     /locus_tag="VFV61_05955"
                     /inference="COORDINATES: protein motif:HMM:NF018434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX4900057.1"
                     /translation="MTSGKRIVLTTFGSFGDVHPYMAIALELRQRGHHPVIASSELYR
                     EKMLASNFEFVPVSPHIPPPQEQDPEMMEKVMHPRLGSGFLLHEMLFPYVREGYAELL
                     QAVAGADLLVTHPITFAGPLVAQKTGIPWVSTVLAPASFFSAYDPPRPPFWPWLRHLS
                     ILGPRFMTWFLNRAKMVYRSETYEQFRRELGLPDRGHPLFEGAHSPDLVLALFSELFA
                     QPQPDWPPNARATGFAFYDGRNELEMPGELIDFLDNGPPPIIFTLGTSAVWVARDFFR
                     ESIAATQLLKRRAVLLIGDERNKITESLPPEIIAVEYAPFESLLGRGCAMIHHGGVGT
                     TAQGLRAGIPTLIVPFAFDQPDNAAHAERLGTSRTLPRSKYRRDKVARELDKLLNIPQ
                     YALKAKSVGEQLRNEHGAARACDAIEEFLEQLPPPENRKRELTYAAGN"
     gene            4269..5045
                     /locus_tag="VFV61_05960"
     CDS             4269..5045
                     /locus_tag="VFV61_05960"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011996181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEX4900058.1"
                     /translation="MLLVIDVGNTNTSLGVYRDAELVAHWRLTTNPARTVDEYGVHAR
                     NLFQLAALDFKAIDAIAIASVVPPLNYTLKTMAETYFQLSPLFVDSNTNTGLKILYEP
                     ATDVGADRIVDAVAAIEKYGAPCIIVDFGTATTFDVINDQGEYLGGVITPGIMISADA
                     LFERAAKLPRVDIKRPQRVIGLSTTTAMQSGLYHGYTSLVDGVLKKIREELGQSGKPP
                     RVIATGGLASLIATGSEYIEEVDDTLTLDGLRLIYERVQK"
     gene            5139..>5825
                     /locus_tag="VFV61_05965"
     CDS             5139..>5825
                     /locus_tag="VFV61_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900059.1"
                     /translation="MASPQNYFNYFTEIEDAFVRRRGKHLMLSPMDWALIERWKELNV
                     PLHIVLRGIEQSFDSWEAKPRKRTVKSLLYCQEEIEAQYAEWLESRVGAAIEEGVAPA
                     GKEEGLPFPRQAVLEHMQRGCQALQTVAKDLNEGELAETISRVVPLLEALESDFATAA
                     IPDARQLEISLTGLERMLSDSLKSAVSQAQLSAAQTEIKEQLRPYRSHMDKAAYEQTF
                     NNLLLKRLRED"
BASE COUNT         1309 a         1583 c         1651 g         1282 t
ORIGIN      
        1 ggccacaagt ggttcatcag cggcgcgctt ggttcagcat tcgcgattgt gatggcagtc
       61 actgacccaa acgcggaagc acacgcccgc gccagtatga tcctcgtacc gactgacact
      121 cccgggttca atatcatcag ggcagtcccg gtgatgggat ccggtggtgt cggtggccat
      181 tgcgagatcc gcttcgaaga ctgtcgcgtg ccgctaagca atctgctggg agaccgcggg
      241 aagggtttca agctcgccca ggcccggctt ggtccgggaa gaatccagca ttgcatgcgc
      301 tggatcggcg cggcgcaacg cagttttgaa atgatgtgtg agtatgccct ggagcgggag
      361 tcgtttggcg aaccgctggc gaacaaacag acggtgcaaa actggattgc cgactcggct
      421 gcggagattg aagccgcccg cctactgacg ttgaaagcgg cttggaaact tgatcgcgag
      481 gaagatgcgc gcattgagat ttcagtgatc aagttttttg ccgcgcgggt cctgcacgat
      541 gtgatcgacc gtgcgattca ggtccacggc gcgctcggtt attcgaaaga tacgccgctc
      601 gagatgttct atcgagacgc acgtgccgcg agaatttatg acggtcctga cgaagtgcac
      661 cggcaagcag tagctcaacg agttttgaag gcgttcaagc gcgagtagtc agtagttagt
      721 gctcacaagg ccagctaaag aatcagaggg ataaccaacc accgctcaac accgagccac
      781 tgaccactga caactaacta ctgacaacca acaactgtcc actgactact tactcaggat
      841 gacgtttatt aaagatagta tcgccctgcg acccggtgag gagttggatc ttgaaacttt
      901 ggatcactat cttcggcgac agctctcgac tctaactttt gatccgggca gcgcactcga
      961 actgggacaa tttccgggtg gtcattcgaa cctgacttac ctggttcgtt acggcgagaa
     1021 agaatttgtt ttgcgccgac cgcccatcgg tccggtcgcg cccacggcgc acgacatgcc
     1081 gcgcgagtac aacttgctct gtgtgatcca tcctcatttt ccgctggcgc ctaaaccgtt
     1141 cctgctttgt gaagacgtag cggtgatcgg ggcgccgttc tatttgatgg aacgcaggca
     1201 gggtctgatc gtgcgccagt cgttgcccgg gccactcagc gacgacctca tcgcgcgccg
     1261 gcagttgagc gaaacagtgg tagacacact cgccgcactc catgcggtgg acatctacgc
     1321 gaccggtatc gtgagtattg ggaaacctga gggactggtg cgtcgtcagg tccacggttg
     1381 gaccgagcgt tggcgacgct caaagaccgg cgacttagat caaatggaaa gagtcatcga
     1441 gtggctcgag cagcgcatgc ctcccgaagc cgacggcaca gcggcgacca ttgttcacaa
     1501 cgatttcaaa ctcgacaacc tgatgctggc cggtgacgac ccgacaagcg tgatcgccgt
     1561 actcgactgg gaaatgacaa cagtcggcga cccgctgatc gacctcgggc tgttgcttac
     1621 gtattggacg atgcgcggtg ctaaagacag tgaacagaat cgttcgttga ctgcggtcac
     1681 aaacgggccg ggctggttta cacgcgagga gattcttgcg cgctaccagg cgaaaaccgg
     1741 acgggacctt tcgcgtatcg tcttctacga agtctttgcg cgcttcaaaa tcgccgtagt
     1801 cattcagcag atctacttcc gttatgtaaa aggacagacg caggatcaga gattccgcac
     1861 gctcgatgtg ctcgtcaatg cactggcctg cgaggcgttg acgctggcgg aaggccgaaa
     1921 ggcttaagtc gtttttggca cgctgaaagc gtgcaagcca atagccgggg gcgagcgttt
     1981 cgcgacgccc ccgggaatcg atcgccgaat aacccgaccc tgacagggtc gaacattctt
     2041 tgaccctttc aggatcgaaa gaaattttac cgcgatggaa ccgtgagcgt tacccacggc
     2101 tattaactct cacgctttca gcgtgaagac ccggcgagtg gctcgtcgaa tattgttaac
     2161 atgcccgatc ttcagtttca caccctacgc tttgactcgc tgccttcgac gaatctcgaa
     2221 gccgcgcggc gcgctcaaga aggagcgcct gaaggtcttt gcatcgtggc gagtgaacaa
     2281 actgccggcc gtggacgcct cggtcgccaa tgggtctcgc cgcgtaatgc gggaatctac
     2341 tgcagcacct tgttgcgccc gcagttcgat ccgagctcgt ggccattgat caccttgatg
     2401 gccgcgattg tcgtcaacga agcattgttg gagacatgtg ctctaactac agacataaaa
     2461 tggcccaacg atattcttta tgaagagaag aagttgtgcg gcatcctcgc ggagaccgtg
     2521 gaaacgtcta cgggacgggc gttagtcgtc ggcatcggca tcaatctgac taacgattct
     2581 tttccgcctg agcttgcggc aacagcgaca tcggtatccg tagcgaccgg cagccctccc
     2641 aacttggagg atgtgctcag caaactacag caatgttttg ttgtttggta tcaccgactg
     2701 aatcaaccaa caggcgcagg cgatatcatc cgcgcctggt ctgaccactc ctcttatgcc
     2761 gacggcaaaa agatcatgat tagggacgct gatacggagt tttgtggaac gacgcgcggg
     2821 ataacggtgg acggtgctct gaaagtagaa agcgaaaccg gtgagattaa gatattccga
     2881 gcaggtgatg tcagcttacg ctcgggatag ggcctagccc ataagtttgt cgaaacatac
     2941 tcaagcataa gagtcgatcc cgatgacctc tggcaaacga atagtgctaa cgaccttcgg
     3001 ctccttcggc gacgtccatc cctacatggc gatcgctttg gagctgcggc agcggggaca
     3061 tcatccggtg atcgccagca gcgaactcta tcgcgagaaa atgttggctt cgaatttcga
     3121 gttcgttccc gtgagcccgc acatcccgcc gccgcaggaa caagatcccg aaatgatgga
     3181 aaaggtgatg catccccgac tcgggagcgg attcctcctg cacgaaatgc tgtttccata
     3241 cgtccgcgaa ggatatgcgg agcttctgca ggccgtggct ggcgccgact tgcttgtgac
     3301 acatcccata acttttgccg gaccgctggt tgcacagaag acaggtattc cctgggtgtc
     3361 cactgtctta gccccggctt cgtttttctc tgcttacgat ccgcccaggc caccattctg
     3421 gccgtggctg cggcacctga gcattcttgg gccacgcttt atgacttggt tccttaatcg
     3481 agcaaaaatg gtttacagga gtgagacgta cgaacaattt cgccgcgaac tgggtttacc
     3541 cgatcgcggg caccctttat ttgaaggagc ccactcgcct gatctcgtgc tcgccctctt
     3601 ttcagaattg ttcgcgcaac cacaacccga ttggccacca aacgcccgag cgacaggatt
     3661 cgccttttac gacgggcgga atgaactaga gatgcctgga gaactcattg atttcctcga
     3721 caacggcccg ccgccaatca ttttcacgtt gggaacttca gcggtttggg tcgcgcgcga
     3781 tttctttcgc gagagcattg cagctacaca acttctcaag cgccgagccg tgctcttgat
     3841 tggtgatgaa cggaacaaaa ttacggaatc gctgccgccg gagattattg ccgtcgaata
     3901 tgcgcccttc gagtcgttgc tggggcgggg ttgcgcaatg atccatcatg gcggcgttgg
     3961 tacgacagcg cagggcctac gcgccgggat tcccacgttg atcgttccct tcgctttcga
     4021 tcaaccggat aacgcggccc acgcggaacg actcggcacc agtcgtacgc ttcctcgcag
     4081 taaatacaga cgtgataagg tggcccgcga gctcgacaag cttttgaata ttccgcagta
     4141 tgctttgaaa gccaaaagtg ttggcgagca attgcgaaac gagcacggtg cggcgcgcgc
     4201 ttgcgatgcg attgaggaat ttctcgagca actcccgccg ccggaaaaca ggaaacgaga
     4261 gctgacctat gctgctggta attgatgttg gcaacaccaa cacgtcatta ggcgtctatc
     4321 gcgacgccga gctcgtagcg cactggcgtc ttaccacaaa ccccgcacgt actgttgacg
     4381 agtatggcgt tcatgcgcgc aacctttttc agcttgccgc tctcgatttt aaggctatcg
     4441 atgcgatcgc catcgcctct gtggtgccgc cgctcaacta caccctgaag acaatggcgg
     4501 agacttactt ccagctgtcg ccgctgttcg tcgacagcaa tacaaacacc ggactcaaga
     4561 tcctctatga gccagcaact gacgtgggcg ctgatcgcat cgttgatgcg gtggcagcga
     4621 ttgagaagta cggagcgccg tgtatcatcg tcgacttcgg cacggctacg acttttgatg
     4681 taattaatga ccagggcgaa tatctcggcg gcgtaatcac accaggaatt atgatctcgg
     4741 ctgacgctct ctttgagcgc gccgcgaaat tgccgcgcgt tgacatcaaa cgtccgcagc
     4801 gcgtgattgg attgtcaact acgacggcca tgcagtcagg tctctaccac ggttacactt
     4861 cgctggttga tggtgtcttg aaaaagatca gggaagagtt ggggcagagc ggaaagccac
     4921 cgcgtgtaat cgcgactggt ggactggcct ctttgatcgc caccggttcc gagtacattg
     4981 aagaggttga cgacacgcta acgctcgatg gcctgcgctt gatttacgaa cgggttcaaa
     5041 agtgatatgc aaaccgccaa gtcgccagga agggtggttt acccccgctt agtcgttcgt
     5101 tgttcagcat ccattctgct cacttaacag aatcggcaat ggcttcgccc caaaattatt
     5161 tcaactactt cacggaaatc gaagacgctt tcgtgcggcg ccgcggcaag catctcatgc
     5221 tgagcccgat ggactgggct ctgatcgagc gctggaagga gctgaacgtt cctttgcaca
     5281 ttgtgctgcg cggaatcgaa caatcgtttg actcctggga agcaaagcca cgtaagcgaa
     5341 ctgttaagtc actcctttat tgtcaggaag agatcgaagc gcagtatgcc gagtggttgg
     5401 aatcgcgggt cggcgcggcg atcgaagagg gagttgcacc ggcgggcaag gaggaaggtc
     5461 taccgtttcc gcgccaggcg gtgctcgagc acatgcagcg cggctgccag gcgttgcaga
     5521 cagttgcgaa agacctaaac gaaggtgagc tggcggaaac gattagtcgc gttgtacctc
     5581 tgctcgaggc tttggaaagc gacttcgcta cggctgctat tcccgatgcg cggcaacttg
     5641 aaatttcgtt gactggatta gaacgaatgt tgagcgattc tttgaagtca gctgtttctc
     5701 aggcccagtt gtcggcagcg caaactgaaa taaaggagca gttgcgacca tatcgcagtc
     5761 acatggacaa ggcggcttat gaacagactt tcaataatct tttgttgaag cgtctgcggg
     5821 aggac
//
LOCUS       DASUEX010000356         3294 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_741188, whole genome shotgun sequence.
ACCESSION   DASUEX010000356 DASUEX010000000
VERSION     DASUEX010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3294
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_741188"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="VFV61_05970"
     CDS             complement(<1..542)
                     /locus_tag="VFV61_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900060.1"
                     /translation="MTDSEKKRISVFSLGLCVAAILYLALAPLANALAWSQWRRPGFE
                     VKVPAEFALQSQQGSPADDGSFVVVLGFKLTKSRRAIPRTDATVTAPAATVTRADIDY
                     EKSKRANDPVGAPDPRSYSVDARSAALEKIVQESRSAPAKTLEGFEHRVKVKNAGTKA
                     VEVLFWEYQLIESLNPANVTR"
     gene            937..1761
                     /locus_tag="VFV61_05975"
     CDS             937..1761
                     /locus_tag="VFV61_05975"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX4900061.1"
                     /translation="MTEELKPVKKKRREEALPERLLRRVFEGMGDVVDRKFGRTVAPK
                     SGLTTSQLIDRMKRLIDERVRDEGRKGRIAPHHLKLKVEWGTHSEAPPEILQELEHEV
                     LAEAIDHINDHRYRTLAPIKVETEVDIFTTGVSVDPTFGEFEEELRQEDEAKHAGSMK
                     VPHGAPPMPDVIVVARITNRERTMETPLTFKPGGRRLNVGRVSDNDLSFNDNSVSKIH
                     ATLMMNQEGTLMVADTGSTNGTFINGRRISYGEARQIEDGDVVSFGDVEVRFRKTD"
     gene            1784..>3294
                     /locus_tag="VFV61_05980"
     CDS             1784..>3294
                     /locus_tag="VFV61_05980"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEX4900062.1"
                     /translation="MSLLFLLQTPSPTPVVVSAPERMPALLTLIYLGGFSFLILILLV
                     SLFRNRRRSSAPTATLPADLPQAVRRRLGSTSTNRGLRALRWIFVLLAAAMFGFHIYW
                     AHYAEESNVKFQELSYKDLRNRRLSESTLRGWLLDRSGKLDKALAYYHRDGSGNIVRE
                     YPMDKALAHIFGTDRGDPGLERALFGVQSGALPEALQVVRRETPQNKGNTDVKLTIDR
                     ELQQAAVDQLKGKHGAVVILNPQTGEVLALYSEPSYSIKDVQDETTWLKLEANQRDRP
                     LVSRALGAYYIPGSTFKTVTMTAAFIAGIQDTEFVCSGGGYYAAQGANVIYDDGGPGE
                     VHGRIGIDTAYEVSCNQYFAQMGVKLGAERLKQAAQLLGIGAYDTPAEALRGRKEPEL
                     WNASSDAVKRALAPREATIVTGKQVTRYDLALIGYGQGYAGQMTPFQMALAAAAIGNM
                     EGKLMKPKIELDRPPEVFKQVVSQQNAQRMRQIMGLVTGGPSGTARGVFGPVKA"
BASE COUNT          735 a          922 c          916 g          721 t
ORIGIN      
        1 cgcgttacat tcgctggatt cagcgactcg atgagttgat actcccagaa taagacttca
       61 acggctttgg tgcccgcgtt tttgactttt acccggtgct caaacccctc gagagtctta
      121 gccggagcgg aacgggattc ctggacgatc ttttcgagag ctgcgctgcg cgcatctaca
      181 ctgtagctcc ttgggtcggg cgcccctacg ggatcattcg ctcgcttact tttctcgtag
      241 tctatgtccg ctcgcgtcac ggtcgcagct ggcgcggtga ctgttgcgtc cgtcctggga
      301 atcgccctcc gactcttcgt caacttgaaa ccaagcacaa caacaaaaga tccatcgtcc
      361 gcgggcgacc cctgctgact ttgcaaagcg aactcagccg ggaccttaac ttcgaagcct
      421 ggcctacgcc attggctcca agccagggcg ttggccaacg gcgccagagc tagatacaga
      481 atcgcggcga cgcagaggcc cagtgagaaa actgagattc tcttcttttc agaatcggtc
      541 atgcccatcc tcctatggaa cgcacgaaca gacttatcac ttgtgttgtt gtttcttggg
      601 gagatttgct gcttcgagag tccgaccctg atgccggtag ataatactcc tcgaagtcgc
      661 gggtgataag cttttccctt cagcctcctt gaaacggctg gcgccggtag cgtgcttctc
      721 cctattgcca attgcgcctt taagttagaa tgacggccat agaaagccac acttgaaccc
      781 gaacccatta ccgattgcgc cacgcaccga atggtagctg aatccactta ttctcttcct
      841 gctgtgcacc tgccgttcag cagtggcatt tcctctggtt gaatgaagcg aattcgaacc
      901 gggaacctgc gttagcctgc ggcgtattta gttaacatga ccgaagaact caagccggta
      961 aaaaagaaac gacgtgaaga agcgctcccc gagcgactgc tccgccgcgt gtttgaaggc
     1021 atgggcgacg tggttgatcg caagtttggc agaactgtcg ccccaaaaag tggattaact
     1081 acttcacagt tgatcgaccg catgaagcgt ttgattgacg agcgcgtgcg cgacgaagga
     1141 cgcaagggac gcatcgcgcc acaccatctg aagttaaaag ttgaatgggg cactcactct
     1201 gaagccccgc ctgagatact tcaggagctc gagcatgagg tgcttgccga agccattgat
     1261 cacatcaacg accaccgtta tcgcactctg gctccgatca aagtagaaac cgaagtagac
     1321 atcttcacta ccggggtttc tgtcgatccg acctttggag aatttgaaga agagttacgg
     1381 caggaagatg aggcaaagca cgccgggagc atgaaggttc cacatggcgc tccgccgatg
     1441 ccggacgtta tcgtggtggc gcgtattacg aatcgcgagc gcaccatgga aacgccgctt
     1501 actttcaaac ccggcgggcg ccgcctgaat gtcggccgcg tttccgacaa tgatctttcg
     1561 tttaatgaca acagcgtttc aaaaattcac gcgacgttga tgatgaatca ggaaggcacg
     1621 ctcatggttg ctgacactgg ttccactaac ggcacgttca taaacggtcg ccggatttct
     1681 tatggcgaag cgcggcagat tgaagatggc gacgtcgtga gctttggtga cgtcgaagtt
     1741 cgctttcgga agactgactg aagcaaggtt cgcatcgttg atcatgtcgc ttctcttctt
     1801 gctgcaaaca ccatcaccga cgcccgtcgt tgtttcagct cccgagcgca tgcccgccct
     1861 gcttactctg atctacctgg gtggtttttc cttcctgatc ctgattctct tggtctcact
     1921 tttccgaaat cgcaggcgca gctcggcgcc gaccgccacc ttacctgccg acctgccgca
     1981 agctgtacgc cgacggctcg gctccacttc caccaatcgc gggttgcgcg ccttgcgttg
     2041 gatcttcgtg ctgctcgccg ccgccatgtt tggctttcat atttactggg cccactacgc
     2101 cgaggagtcg aacgtaaagt ttcaggaact tagttacaaa gacttgcgga atcgacggct
     2161 atcggaatcg acgcttcgcg gctggctgtt ggatcgcagc ggaaaactcg acaaagcgct
     2221 cgcctactat caccgcgatg gtagcggcaa catcgtgcgc gagtatccga tggacaaagc
     2281 cctggcgcac atctttggca ccgatcgcgg tgatcccggt ttggagcgag cgctgtttgg
     2341 ggttcaaagc ggagccttgc cggaagcgct gcaagtggtg cgcagggaga cgccccaaaa
     2401 caaaggcaac acggatgtga agctaacgat tgatcgcgag ctgcaacagg ccgcggtcga
     2461 tcagctcaag gggaaacatg gcgcggtggt aatcctgaat ccgcagactg gcgaagttct
     2521 ggctctttac agcgagccgt cttacagcat caaggacgtt caggatgaaa ctacctggct
     2581 taagctcgaa gccaaccaac gcgatcggcc cttagtgagt cgcgccctcg gcgcctacta
     2641 catacccgga tcgacgttca agaccgtgac gatgacagcg gccttcattg ccggaatcca
     2701 ggatacagag ttcgtttgca gtggcggcgg ttactatgcg gcgcaaggtg cgaatgtgat
     2761 ctatgacgac ggtgggcccg gtgaagttca cggtcgcatc ggtatcgata ccgcctacga
     2821 agtctcttgt aatcaatatt tcgcgcaaat gggtgtgaag ctgggagccg agcgactcaa
     2881 acaagccgcg caactgctcg gcatcggcgc ctacgatacg cctgcggaag ctttgcgggg
     2941 acgcaaagag cctgaacttt ggaacgcgag cagcgacgct gtaaagcgtg ctctcgcccc
     3001 gcgcgaggca accatcgtta ctggtaagca agtcacgcgt tacgacctgg cgttgatcgg
     3061 atacggtcaa gggtatgccg gacagatgac gccgtttcag atggcgctgg ctgccgcagc
     3121 aattgggaac atggaaggca agttgatgaa gccgaagatt gaacttgacc gacccccgga
     3181 agtcttcaaa caagtcgtgt cacaacaaaa tgcgcaacgc atgcgacaaa tcatgggact
     3241 cgtcactggc ggccccagcg gtacagctcg gggcgtgttc ggtccggtga aggc
//
LOCUS       DASUEX010000357         2485 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1441074, whole genome shotgun sequence.
ACCESSION   DASUEX010000357 DASUEX010000000
VERSION     DASUEX010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2485
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1441074"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1013)
                     /locus_tag="VFV61_05985"
     CDS             complement(<1..1013)
                     /locus_tag="VFV61_05985"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX4900063.1"
                     /translation="MPTYVFKGRNKLNETVVGERVADNRDALRQILRREQVTLTSVKE
                     KGREVGIPKLGGRKKVKAKVLSIFTRQFSVMIDAGLPLVQCLEILAQQQDNKYFQATL
                     MQVRQDVEEGSTLSAAMARHPKVFDQLYTNMVEAGETGGILDLILQRLSSFIEKIVKL
                     KRDVISAMIYPAAVVLMAVGAVAVIMIIVIPQFQNIFLGLLGPGEQLPLPTRIVVGIS
                     NFLAGWGGLTILIALIAAVLFVRFYYKTPNGRRTIDRLLLKIPILGDILRKVAVARFS
                     RTLSTLLSSGVPILQSLEITARTAGNVIIEEAILKVRAGVERGESFVDPLKATEVFPH
                     MVA"
     gene            complement(1039..2238)
                     /locus_tag="VFV61_05990"
     CDS             complement(1039..2238)
                     /locus_tag="VFV61_05990"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HEX4900064.1"
                     /translation="MRINEGAPQISLSDLLKKLSELRGSDLHITTGSQPQVRVDGHLS
                     PLDGYRVLTSADTKQLAYSVLTDAQKHRFEENLELDFSFGVKGLSRFRANLFNQRGAV
                     GAVFRAIPYEIKAFEELGLPAVVKQMCDKPRGLILVTGPTGSGKSTTLASMVDKINRD
                     RHEHILTIEDPIEFLHNHKNCVVNQREVNADTHGFAEALRTALRQDPDVVLVGEMRDL
                     ETIESALRIAETGHLTLATLHTNSAASTINRIIDVFPSDQQAQIRAQLSLVLEGILCQ
                     ALLPRAHGGGRVMALEILVPNGAIRNLIREDKVHQIYSMMQTGQDKYGMQTFNQALAT
                     LYHKRLITADIAMQRSSNVDELKELMDRGSGVNTGNGGHQPPAPGNHPSPYAQARPVG
                     ARPTITK"
     gene            complement(2293..>2485)
                     /locus_tag="VFV61_05995"
     CDS             complement(2293..>2485)
                     /locus_tag="VFV61_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900065.1"
                     /translation="IVTAQTPGDGRNPDKSWNFYFTEVNGRIYRLTTNTPQQFSERMA
                     AEAESFIASFHAISKPASK"
BASE COUNT          548 a          658 c          719 g          560 t
ORIGIN      
        1 tgggccacca tatgcgggaa tacttcggta gctttcaggg gatcgacgaa gctctcacca
       61 cgttcgactc cggcgcgaac tttgagaatc gcctcttcaa taatcacgtt gccggcggtg
      121 cgcgccgtaa tttccagtga ctggagaatc ggcacgccgg aggagagcaa ggttgaaagc
      181 gttcgggaga atctggcaac tgccactttg cgcagaatgt cgcccaggat gggaatctta
      241 agcagtaacc gatcgatggt gcgtctgccg ttgggtgtct tgtagtagaa cctcacaaaa
      301 aggacggccg ctatcagagc gatcaggatt gtcagaccgc cccaaccagc caggaagtta
      361 ctgataccga caacaattcg tgtcggcagc ggcaactgtt cgcccggtcc caatagaccc
      421 agaaagatat tttgaaactg cggaattaca atgatcatga tcactgccac cgcaccgacc
      481 gccatcaaca caaccgccgc cggatagatc atcgcggaga tcacatctcg tttcaatttc
      541 acgatctttt cgatgaacga tgagagacgt tgcaggatca ggtcgagaat accgcctgtc
      601 tcgccggctt cgaccatgtt cgtgtagagc tgatcaaaga ctttgggatg gcgcgccatc
      661 gctgcggaaa gcgttgaacc ctcttccacg tcctgacgga cctgcatcag cgtcgcctga
      721 aagtacttgt tgtcctgttg ctgcgcgaga atctcgagac actggaccag cggcaagccg
      781 gcatcgatca tcactgagaa ctgccgcgtg aaaattgata gaacctttgc tttaactttc
      841 ttgcggccac cgagtttggg aatgcctact tcacggccct tctccttcac ggaggttaac
      901 gtgacttgct cgcgccgcag gatttggcgc aacgcgtcac gattatccgc aacgcgttcg
      961 ccaacgacag tctcgtttag cttgttgcgg cctttgaaaa cataggtagg catagtctag
     1021 agctccattg tcgaggattt acttggtgat ggtggggcgt gcgcctacgg ggcgtgcctg
     1081 ggcgtaaggg ctgggatgat tgcccggggc gggtggttga tgaccgccgt ttccggtgtt
     1141 aacgccggaa ccgcgatcca ttagttcctt cagctcatcg acattcgagg accgctgcat
     1201 tgcgatgtca gccgtgatca agcgcttgtg atagagcgtc gccagagcct gattgaaagt
     1261 ctgcattcca tacttatctt gaccggtctg catcatcgaa tagatctggt gcaccttgtc
     1321 ttcacgaatc agattgcgaa tggcgccgtt cggcaccagt atttccaacg ccatcacccg
     1381 accacctcca tgagcgcgcg gcaacagcgc ctggcagaga atgccttcca gcacgagaga
     1441 aagctgcgcc cggatctgag cctgctggtc agaagggaag acgtcgatga tgcggttgat
     1501 tgtcgaggcc gctgaatttg tgtggagcgt ggcgagcgta aggtggcccg tttcagcaat
     1561 gcgcagggct gactcaatcg tctccaggtc gcgcatttca ccgaccagca ccacgtctgg
     1621 atcctgacgc agcgccgtgc gcagcgcttc agcaaagcca tgcgtgtcgg cattgacttc
     1681 acgttgattg accacgcagt tcttgtggtt atggagaaac tcgattggat cttcgatcgt
     1741 caaaatgtgc tcgtgacgat cgcgattgat cttgtcgacc attgaagcca gcgtcgtcga
     1801 cttgcccgat ccggttggtc cggtgacgag aatcaggcca cgaggcttgt cgcacatctg
     1861 cttcaccacc gcgggcaaac cgagttcttc gaatgctttg atctcgtagg ggatggcacg
     1921 gaacacggcg ccgacagcgc cccgctggtt aaagaggttg gcgcgaaaac gcgaaaggcc
     1981 cttaacaccg aaagagaaat cgagctcgag attctcctca aagcggtgct tttgggcgtc
     2041 cgtcaagact gagtaggcca gctgtttcgt atcggcagaa gtgaggacgc ggtaaccatc
     2101 gagcgggctg aggtgaccat cgacgcggac ttgaggctga gagccggtgg tgatgtgcag
     2161 gtccgaaccg cgcagctcag agagcttttt gagcagatcg gacaaggaga tttggggggc
     2221 gccctcattt attcgcatcg agcaattttc ctccgagagt aaggcactga aggtctgggt
     2281 ccggctgtcc agttacttac tggcaggttt ggaaatcgca tggaaagacg cgatgaagct
     2341 ttccgcttcc gcagccatgc gctctgaaaa ctgttgcggg gtattagtcg tcagtctgta
     2401 gatgcgacca ttgacttcgg taaagtagaa gttccatgac ttgtcgggat tgcgaccgtc
     2461 acccggagtt tgagcagtga cgatg
//
LOCUS       DASUEX010000358         3229 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_869441, whole genome shotgun sequence.
ACCESSION   DASUEX010000358 DASUEX010000000
VERSION     DASUEX010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3229
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_869441"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="VFV61_06000"
     CDS             complement(<1..463)
                     /locus_tag="VFV61_06000"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HEX4900066.1"
                     /translation="MEVKLTALQKDYAELHTAIFEAAQVHRRLCAPRLVRDRDFEIAS
                     EIFAVRHLPGDFFTIKKASGSVILALGDICGKGLAAGMWTTHLVGLVAAHAAVNTDPE
                     SIVAAVNRDVCRMGPVAPLASLFLAKLDPFTGRLEYCSAGHPPALFLRASGR"
     gene            complement(625..1314)
                     /locus_tag="VFV61_06005"
     CDS             complement(625..1314)
                     /locus_tag="VFV61_06005"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX4900067.1"
                     /translation="MSRRTRILIVEDEPAMVAGLRDNFEYEGYEVISAADGLAGLDRA
                     LADEPDLVVLDVMMPRMSGLDVCKQLKIKRPALPIIMLTARGQEIDKVVGLELGADDY
                     VTKPFSIRELMARVKAVLRRASPQVPAADVYRFSDIEVNIRSNEVKRAGEPIELSAKE
                     FALLAYFVSHPVETLSRDRLLDAVWGYENYPNTRTVDTHIVHLRQKLEPNPEEPRFIL
                     TVHGSGYKFVG"
     gene            complement(1435..3120)
                     /locus_tag="VFV61_06010"
     CDS             complement(1435..3120)
                     /locus_tag="VFV61_06010"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX4900068.1"
                     /translation="MKLKRRGFLGGFNRFRLMLTLGLAVVLPAAALIYVNFSQLRAFE
                     RDKVLEAAIHRDFQEILAITEKKINKKVYAMTDEVRSRFPSPDTEPQEKEKKLDLILA
                     QNPWLTHVFLFGEKDFVFRSQPQQMTDKYIRAEHERMVETYRGWFGMEGKMLVESMHK
                     KPRPMNFYTGPTKRSDGEAYVTTAVFALPQLSNDRVVVGGVTFDSCYLKNTFFPGMLE
                     ELVNLKMNDQGGNQIAMMVYPADVEGGSQFKPIATSPGWGEGKPEVERKFDDVFRGLA
                     LGIKYQGTSVQALGETWVHRSFLILGFLSLLIIGGLVLTKRIVSKEMALARLKSDFVS
                     NVSHELRTPLALIRLYAETLELGRINTKEKKQQYYRIIRKESERLTALINNILDFSRI
                     EAGRKEYEFRNTDIAELVHNTLESYRYQIEQQGFAFEESIDSDLPAVPVDREAIARAL
                     VNLVNNALKYSTEEKFLGVKLYRENGVVKLEVADHGIGISRRDQSKIFEKFYRAGDPL
                     VHNTKGSGLGLSLVRHITQAHGGEIAVESTPGRGSKFILSLPLASPSYQTGVS"
BASE COUNT          688 a          942 c          836 g          763 t
ORIGIN      
        1 gcctgccgct ggcgcgcagg aataatgcgg gtgggtgtcc ggcactgcag tactccagtc
       61 ttccggtgaa aggatcaagc ttggccaaaa acaagctggc cagcggcgcc accggtccca
      121 tccggcaaac atcgcggttc acggcggcga cgattgattc cggatcggtg ttgacggccg
      181 cgtgcgccgc gaccagaccg accaggtgcg tggtccacat tccggccgcc agtcccttgc
      241 cgcagatgtc ccccagcgcg aggattactg agccgctcgc cttcttaatc gtaaagaagt
      301 cgcccggcag gtggcggacc gcaaagatct cgctggctat ttcaaagtcg cgatcgcgga
      361 ccagtcgagg ggcgcaaagc cggcgatgca cctgcgccgc ttcaaagatg gccgtatgaa
      421 gttcggcata gtctttctgc agcgccgtca gctttacctc caaatcttgc ttgccggttc
      481 gactcggacg tcggcgaggc cgcgaagaca ctcggacatc gactgagcgc tgagttgcta
      541 acccgctatt taatcttgag tgagccattg ttgtagcacg cgctgccggc gcgtgattca
      601 cgggccggcg gcccgtgtta cctttcaacc aacgaatttg taaccactgc cgtgaacggt
      661 aagaataaaa cggggctcct caggattcgg ttccagcttt tgcctgagat gcacgatatg
      721 tgtatcgact gtccgcgtgt ttggataatt ctcgtagccc catacagcgt ccaacaaacg
      781 gtcgcggctg agtgtctcca caggatgtga gacgaaatac gcaagtagcg cgaactcttt
      841 ggctgagagt tcgatcggct cgccggcgcg tttcacttca ttgctgcgaa tgttgacctc
      901 aatatcgctg aatcgataga catccgccgc gggcacttgt ggcgaagcac gtctcaagac
      961 ggcctttacc cgggccatca gttcgcgaat ggagaagggc ttggtgacgt agtcatcggc
     1021 gcccagttcc aggcccacca ccttgtcgat ctcctgaccg cgcgcggtca gcatgatgat
     1081 gggcagcgct ggtcgcttga tcttcagttg cttgcaaacg tccaggccgc tcattcgtgg
     1141 catcatgaca tccagcacga cgagatcggg ttcatcggcg agagcacgat cgagtcctgc
     1201 caagccatct gcagcgctga tcacttcgta gccttcatac tcaaaattgt cgcgcagacc
     1261 agccaccatg gcgggttcat cctcaacgat caagatgcgc gttctcctgc tcaccgttga
     1321 ctctgtaacg tctttgttca tcgatgatcg attgccattc gcgctgccat ttgattgttc
     1381 gaaacttgct tttctcctca tatgcgcctt cctctgccgg cccgggtatc ctgcttaaga
     1441 cactcccgtc tgataactcg gtgatgccag cggcaacgat agaatgaact tactgcccct
     1501 ccctggggtg ctctccaccg cgatctcccc gccgtgcgcc tgggttatgt ggcgcaccag
     1561 tgaaagaccc aacccgcttc ccttggtgtt gtgcaccagc ggatctcccg cccggtaaaa
     1621 cttttcgaag atctttgact ggtcacgtcg cgagatgcca atcccgtgat cggccacctc
     1681 cagttttact acgccgtttt cacggtacag tttcacgccg aggaactttt cctcagtcga
     1741 gtatttcagg gcgttgttaa ccaggttgac aagcgcgcgc gcgattgctt cacggtccac
     1801 cggcacagcc ggcaagtctg aatcaatgct ctcttcaaac gcgaaaccct gttgctcgat
     1861 ctggtagcga taggactcga gcgtgttgtg aaccagctcg gcaatgtcag tgttccggaa
     1921 ctcatactcc ttgcgtcctg cttcgatccg agagaagtcg aggatgttat tgatcagcgc
     1981 agtcagccgt tcgctctctt tacgaatgat gcggtaatac tgttgcttct tttcctttgt
     2041 attgatgcgt cccaactcga gtgtttcggc atacagacgg atcagggcca acggcgtgcg
     2101 caactcatgc gatacgttag aaacaaagtc agatttcaga cgggccaacg ccatttcctt
     2161 gctcacgatg cgtttggtga gcaccagtcc gccgattatc aatagcgaaa ggaagcctag
     2221 aatcagaaaa ctgcgatgaa cccaggtctc accgagcgcc tgtacgctcg ttccctgata
     2281 tttaatgccc agggccagcc cgcgaaagac gtcatcaaac ttgcgttcaa cttcaggctt
     2341 cccctcaccc caacctggcg aagtggcaat gggtttgaac tggcttccac cctctacgtc
     2401 cgccggatag accatcattg caatctggtt tccgccctgg tcgttcatct tcaggttgac
     2461 caattcctct agcattcccg gaaagaaagt attcttcaga taacaagaat cgaaggtcac
     2521 gccaccgacc acaacccgat cgttcgatag ctgcggtaaa gcaaacacag ccgtcgttac
     2581 ataagcctcg ccatcggacc gttttgttgg accagtgtag aaattcatgg gccgcggctt
     2641 tttgtgcatt gactccacta acatcttgcc ttccatgcca aaccagccac gataggtttc
     2701 gaccatgcgc tcatgctctg cccggatata cttgtctgtc atctgctggg gttgcgaacg
     2761 aaagacaaaa tccttttcac cgaagaggaa cacgtgggtc agccacggat tctgtgccag
     2821 gatcaggtcg agctttttct ctttttcctg cggctcagta tctggcgagg ggaatcgact
     2881 ccgcacctcg tcggtcatgg catagacctt cttgttgatc ttcttttcgg tgatagcgag
     2941 tatctcctgg aaatcgcgat ggatggccgc ctccaggact ttgtcgcgtt cgaaggctct
     3001 caactggctg aaattgacgt aaatcagggc ggccgccggc aagaccacag ccaggcccaa
     3061 cgtcaacatc agccggaacc ggttgaagcc ccccagaaaa cctcttcgct tcaatttcat
     3121 tgtcgacctc tgcacaggaa ctgcgttatg cgaagcataa aatactctta ccggcgttag
     3181 gccgtgttaa gagcacgtaa aaatatgtca agaattgtta agtctgggg
//
LOCUS       DASUEX010000359         2406 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_286907, whole genome shotgun sequence.
ACCESSION   DASUEX010000359 DASUEX010000000
VERSION     DASUEX010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286907"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..507
                     /locus_tag="VFV61_06015"
     CDS             70..507
                     /locus_tag="VFV61_06015"
                     /inference="COORDINATES: protein motif:HMM:TIGR04191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HEX4900069.1"
                     /translation="MPYPEIMIRPMREELTRLGAEELRTEADVDRTLQSNEGTVMVVV
                     NSICGCAAGKARPGIALALQHETRPDKVTTVFAGADIEATEKARSYFTGFPPSSPSIA
                     LLKDGKLVYMMQRHQIEGRGPVEIAQELTHAFDEHCRKTEAVV"
     gene            complement(559..963)
                     /locus_tag="VFV61_06020"
     CDS             complement(559..963)
                     /locus_tag="VFV61_06020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900070.1"
                     /translation="MSANDPLGKLRETRLALLHLHKTLLDDEREAYERVHDRIENSYE
                     VLRLVMHDPWFAWLHDLSELIVRIDESLDAKEPPSEVDVAGVMRQARELTTPDENGGE
                     FQQKYFRALQRSPDAVLAHAETTRLINNLKQQ"
     gene            complement(1000..1464)
                     /locus_tag="VFV61_06025"
     CDS             complement(1000..1464)
                     /locus_tag="VFV61_06025"
                     /inference="COORDINATES: protein motif:HMM:NF020908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ family domain-containing protein"
                     /protein_id="HEX4900071.1"
                     /translation="MTKRLESLTEKSIREAIEKGEFDNLPGKGKPLDLSENPFEDPDL
                     RTVHRLLRNAGFAPAWIEERKDIEGQLKLACTTLTRAWRLYGPHGKASNSDAWERNVA
                     EFRVQVAELRQRVQIYNLKAPATIFHKRQLDAEALIEEIKKSGATDDRGRER"
     gene            1674..>2406
                     /locus_tag="VFV61_06030"
     CDS             1674..>2406
                     /locus_tag="VFV61_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900072.1"
                     /translation="MRPPLNLETPINELYKYGIARLGNATSQNLAASLSRFGNRKTVS
                     AVIVEDLLWYLPMRYEDRSNLTTIRDLKDGMEASLELYAKVAGGYQVGKTRAQGKPKL
                     YIFQVTATDRFQSGRPVVVWWFVSGSHARDIIDYYMKRLRRGARFITFGKWEWDERRG
                     TFGLRLQKPADELEMLPALTNDALAETATDDKSDIDEADDPRFAAIHVGRRVPIYRKL
                     GDLSSKRLREIIHALLEAIPVKEIKE"
BASE COUNT          558 a          677 c          606 g          565 t
ORIGIN      
        1 cagaaatgac aaatggaaaa tgttcttgtt agactcaata aacgaaacaa tagttaggag
       61 agttgaaaaa tgccttatcc agaaatcatg atccggccaa tgcgcgaaga actcactcgc
      121 ttaggggcgg aagaactacg cactgaggcg gacgtcgatc gcacactgca aagcaatgaa
      181 ggaacggtaa tggtggtggt gaattcaatc tgcggctgtg cggcgggtaa agcgcgcccg
      241 ggaattgcgc ttgcactgca acacgaaaca cgtccagaca aagtgaccac ggtgttcgcg
      301 ggcgctgaca tcgaagctac tgaaaaagcg cgttcctact tcactggctt tccaccgtcc
      361 tctccgtcaa tcgcactcct gaaggacgga aaacttgttt acatgatgca gcgccatcaa
      421 attgagggac ggggcccggt cgagatcgca caagagctga ctcatgcttt cgacgagcac
      481 tgcaggaaaa ccgaagcggt ggtttagaaa actttcgaac tggtgttagt gattagcccg
      541 gccgtgtcgc cgggcctttc attgttgttt gaggttgttg attagtctcg tggtctcggc
      601 atgggccagc actgcatcag gagagcgttg caatgctcta aagtattttt gttgaaactc
      661 tccgccgttt tcatccggcg ttgtaagttc gcgtgcctgg cgcatcaccc cagcgacatc
      721 aacttcggac ggcggttctt tcgcatccag cgattcatca atccttacga tcaactccga
      781 aagatcgtgc aaccacgcga accacggatc gtgcatcacc aagcgcagca cttcatagct
      841 gttttcaatg cgatcgtgaa cccgctcata agcttcacgc tcgtcatcaa gcaatgtctt
      901 gtgcagatgc agtaacgcca gccttgtttc tcgcaacttc cccagaggat cgttcgcgct
      961 cattgattat cctttctcag aggtaacttt cgacctggct caacgctcac gacctcgatc
     1021 gtctgtcgct ccggactttt taatctcttc aatcagagct tctgcgtcaa gctgccgctt
     1081 atggaaaatc gtcgccggcg ctttcagatt atagatctgg acgcgctgtc gcagttcggc
     1141 gacctgcacg cgaaactcgg ccacattccg ctcccaggcg tcagaatttg aagctttccc
     1201 gtgcgggcca taaagtcgcc aagcgcgagt taaagttgtg cacgccagct taagctgccc
     1261 ttcaatgtcc ttccgttcct ctatccacgc cggggcaaac ccggcgttgc gcagtaaacg
     1321 gtggaccgtt cgcaggtccg gatcctcaaa gggattctcg ctgagatcga gcggcttgcc
     1381 tttgcccggg agattatcaa actcgccctt ttcgatcgct tcgcgaatac tcttctcggt
     1441 aagagactcc aaacgtttcg tcacgtcgtc accacaggct ctgaaaagat agggcccaca
     1501 accaccctga atgatttacg gtaagatacc ctttcaagtt catcaagccg gttgtttata
     1561 tacgtcgact ctgtatccgc tcagcgacct ttggagttgt tgcaaggatt gtgatcagta
     1621 gctcgcttgc cacttgccga gaccctctcg gcgctatgtt atgatcatct tccatgcgcc
     1681 ccccgctcaa cctcgaaact ccgatcaacg aactctacaa gtacggaatc gcccgtcttg
     1741 gtaatgcaac ctcgcaaaac ctcgctgcca gtctctcgcg ttttggaaac cgcaaaaccg
     1801 tcagcgccgt aattgtggaa gatctgcttt ggtacctgcc gatgcgttac gaggatcgct
     1861 caaacctgac gactattcgc gacttgaaag acggcatgga ggcgtcgctc gagctatacg
     1921 ccaaagtcgc cggtggttac caggtgggaa agactcgtgc tcagggaaag ccaaaacttt
     1981 acatctttca ggttaccgct actgacagat tccaaagcgg caggcccgta gttgtctggt
     2041 ggtttgtttc aggctcgcat gctcgcgaca tcatcgacta ctacatgaag cggctgcggc
     2101 gtggtgcccg ctttattacg ttcggcaagt gggagtggga cgaacgccgc ggaacttttg
     2161 gcttgcgact acaaaaaccc gccgacgaac tcgagatgct gccggccttg accaacgacg
     2221 ctctggccga aacggccact gacgacaaga gcgatatcga tgaagcagac gatccgcgtt
     2281 ttgcggccat tcacgttggc cgtcgtgttc ccatctaccg caagctcggc gacctttctt
     2341 ccaaacgtct gcgcgaaatc atccacgcgt tgctcgaggc cattcccgtc aaagaaatca
     2401 aggaga
//
LOCUS       DASUEX010000360         2322 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_636324, whole genome shotgun sequence.
ACCESSION   DASUEX010000360 DASUEX010000000
VERSION     DASUEX010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_636324"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2264)
                     /locus_tag="VFV61_06035"
     CDS             complement(<1..2264)
                     /locus_tag="VFV61_06035"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX4900073.1"
                     /translation="MTITTKRFIAISAFCVLALIVSALNCQAETAASIRGTVTDVRGA
                     KVPGASVQLSSRTGRRIAVTSDENGVFEFKDLNPGEYLLEIDAAGFSHFVSAPLKLGH
                     GATEMNFQLEVAAVTETVIVTATGTAQRADEVAKVVSVVAAQQIESKHESGLAEALRG
                     IPGVRVQQQGSPGTLTSVRLRGQRTFDTSLALDGLRVRDAGDINGSAASLLTDLSPVG
                     LARVEILRGSGSSIYGTNAIGGVLNLVPEIGAGTPHFEIGLEGGALATFRERLKVTGG
                     SQRLGYGFGVNRTDVRRGIDGADEYGSTAGGGRLQFNPTPTVSIDINGYGTISNSRLN
                     DSPVPLPAAFTVGSDYPDAVAGTSFQPDFNNPDQGRRNRLLVGSLRFSQQVRENVSYS
                     VAYQRVSSARRNYNGPRSDPQFASFYPFGDFEFVNISNGTTDTIDARINARLGRSNLV
                     TGGIEFENESAFQQFMPPYTPSNMPRDRQRTFAVFGQDLISLLQGRLQLSLGIRHQSY
                     YLRAADRPGFLESVKTTRSLTGDGAIAYFIRSTNTKLRAHVGNGYRAPSLYERFGIGN
                     FPGAGVTRFGDPTLKAEQSISVDAGFDQRLARDRVLVGATYFYTRLQRVIAFSGFTVD
                     PLGLGRFGGFINQPGGLARGFETFVEATPARGTELRASYTFTNSDRSLATRGLQPEYV
                     IPTHVLGFNWNQRMRAFAINLDVNHNSSHLAPLFENNFPFRSAELRFAGYTKVDLFAR
                     FERRLSERLVMLIFG"
BASE COUNT          534 a          655 c          659 g          474 t
ORIGIN      
        1 ccgaaaatca acataacaag cctctcgctt aagcgccgtt cgaatcgggc aaacagatcc
       61 accttggtgt aaccggcaaa cctgagttcc gcgctgcgga agggaaagtt gttttcaaaa
      121 agtggtgcga ggtgtgatga attgtgattg acatccaggt tgatagcgaa ggcgcgcata
      181 cgttgattcc aattgaagcc cagaacgtga gtgggaatca catactccgg ttgcagtccg
      241 cgagtggcca ggctgcgatc gctattcgtg aaggtatatg aagcgcgcag ttctgtgccc
      301 cgagcgggcg ttgcctcgac aaaggtctcg aacccgcggg ccagaccacc aggttgattg
      361 atgaacccgc cgaatcgtcc cagcccaagt ggatcgacag taaaaccact aaatgcgatc
      421 acgcgctgga gtcgggtgta gaaataagtg gccccgacca gcacgcgatc tctcgccagt
      481 cgctggtcga aaccagcgtc cacgctgatt gattgctctg ccttcaacgt cggatcgcca
      541 aaccgggtca cgcctgcccc cggaaaattg cctataccaa agcgctcata aagcgaagga
      601 gcacggtagc cgttgccgac atgcgcacgt agtttcgtgt tagtggatcg tatgaagtag
      661 gcaattgctc catcgcccgt cagcgatcgc gtggtcttaa cagattccag aaagccgggc
      721 cggtcagcgg cgcgcaagta gtaagactgg tgcctaatgc caagcgagag ttgtagtcgc
      781 ccttgaagga gggaaatcag atcctgaccg aacaccgcaa aggtgcgctg ccggtcgcgc
      841 ggcatgttgg aaggcgtgta cggaggcatg aactgctgaa acgccgattc gttttcgaat
      901 tctattcctc cggtaaccag gttggagcgc cccagacgcg cgttgattcg agcgtcaata
      961 gtgtcagtcg tgccgttgct gatgttgacg aactcaaaat cgccgaacgg atagaatgaa
     1021 gcgaattgcg gatcgcttcg cgggccattg tagttccgcc tggcggatga gacgcgctgg
     1081 taagcaactg agtaggagac attttccctt acttgctgtg agaaacgcag cgagcccacc
     1141 aacaatcgat tacgccgtcc ctggtccgga ttgttaaagt ccggttgaaa actcgtaccg
     1201 gcaacagcgt ccggataatc tgaaccaact gtaaaagcag cggggagcgg gacgggacta
     1261 tcgttcaagc gagagttgga gatagttccg taaccgttaa tgtcgatgga gaccgtaggc
     1321 gttggattga attgaagacg accgccaccc gccgtgcttc cgtattcatc ggcaccgtcg
     1381 attccacgac gcacgtcagt acgattcacg ccaaaaccat agcccaggcg ttggcttccg
     1441 ccggtcacct tgaggcgctc gcgaaacgtc gcgagtgccc cgccttcaag tccgatctca
     1501 aagtgaggtg tacctgcgcc gatttccgga accagattaa gcacgccccc aatcgcattg
     1561 gtgccgtaga ttgaagagcc tgagccacgc aagatttcga cgcgcgcaag gccgaccggc
     1621 gacagatcag taagtagcga agccgccgag ccattgatgt cgccggcgtc gcgcacacgc
     1681 agtccgtcca gcgccaacga cgtgtcaaac gtccgctggc cccgcaggcg aaccgaagta
     1741 agtgtcccgg gtgagccttg ctgctgcact cgcacgccgg gaattccacg caacgcttcg
     1801 gctagacctg actcgtgttt gctctcaatc tgttgtgcag cgacaactga gacaactttg
     1861 gccacttcgt cggcacgctg ggcagttcca gttgcagtca cgatcaccgt ttcagttact
     1921 gccgccactt cgagttggaa attcatctcg gtagccccgt ggcctaactt gagcggcgcg
     1981 gaaacgaagt gcgaaaaccc agccgcgtcg atctccagca aatactctcc cgggttcaaa
     2041 tccttaaact caaaaactcc gttctcgtcg ctcgtgacgg cgatcctgcg accggttcgt
     2101 gaactcagtt gcactgaggc gccaggcact ttcgcgcccc ggacgtcggt aaccgtacct
     2161 cggatcgagg ctgcagtttc tgcctggcaa ttgagtgccg aaacgatcag cgcgagcacg
     2221 cagaaagccg agattgcgat gaatctttta gttgtgattg tcatgttagc ttctcctgcc
     2281 cgtcgcagtg cgagctgaaa gagagcgcag ggccgatgtc ct
//
LOCUS       DASUEX010000361         3792 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_287727, whole genome shotgun sequence.
ACCESSION   DASUEX010000361 DASUEX010000000
VERSION     DASUEX010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3792
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287727"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(226..1770)
                     /locus_tag="VFV61_06040"
     CDS             complement(226..1770)
                     /locus_tag="VFV61_06040"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX4900074.1"
                     /translation="MQLHKSTVSEIGSFEPGLSDEARLQCAQAREQEEAGNFEAARQL
                     LHGFWQRIGERPVLRGLDDAASAEVLLRVGTLTGWIGSAQQIPGAQETAKDLISESAG
                     LFERLGLDDRAAEARIDLGICYWREGALDEARVTFQEVLAQLGERETEQRLRALLNAA
                     VVEKVSVRYRDALRIHQSAAPLFEKSSNHALRGKFHNEFATVLKNIGLAEHREDYIDR
                     ALVEYAAAGFHFEEAGHQRFLGVVENNLGFLFVHLGRYEDAHEHLGRARAVAVALKDQ
                     GLVAQFDDSRAKAFLGEKRYAEAEAISRSSVRSLEQGGEQSLLAGALITYGTSLARIN
                     SSDAALAALKRGMTVAQQAGDPDSAGIAALTIVEELPQFVPNSDLHEYYLQAESLLAR
                     SQHLGVRTRLGECARQILTREKHFSDKPSADSPNGQKLSAARSELRSATLTPGVSLDE
                     LVLNYEGELIRQALETSGGSVTRAARLLGITHQGLAFILNGRHKSLLSIRTPVKRRRR
                     SIIRYR"
     gene            1927..2157
                     /locus_tag="VFV61_06045"
     CDS             1927..2157
                     /locus_tag="VFV61_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900075.1"
                     /translation="MKNVKAMFGTITLGLLLSSVALAGDVSTPGYVPPPPPPAAAPLQ
                     ETGDSDISSSSLAIDSSSLAIDLLIAALGAFY"
     gene            complement(2414..>3792)
                     /locus_tag="VFV61_06050"
     CDS             complement(2414..>3792)
                     /locus_tag="VFV61_06050"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEX4900076.1"
                     /translation="DAGEVAFEAVQERHAANQPRITLAETESLITRLSETRGTKNKTA
                     LLATVLREATPLEAKYLVKLLVGDLRIGLKEGLVEDATARAFGQPLAAVAQANMLLGD
                     IGEAAVRTRSGDLENIEMRLFHPIKFMLATPAADLSDIARTMPEEFFVEDKFDGIRAQ
                     AHVKEGRVSIYSRTMDEITHRFPELVEPLKNLATDVVIDGEIVPATGDRILPFSELQK
                     RLGRKTIGADLLADVPVVLVTYDLLYASGRVLINEVLEERLKTLAELIPSEGTLRLSL
                     AKRFQEVAALDDEFAAARARGNEGLMVKSPASVYKPGRRGREWLKVKRAIATLDVVVT
                     AVEVGHGKRRNLLSDYTFAIRRSLEDDELLNIGKAYSGLTDVELTELTEWFKAHTVKE
                     FAHGRVRLVEPRIVIEVTFDRVQESRRHKSGYALRFPRILRLRTDKTPEEIDTLETVQ
                     RLVAANTQ"
BASE COUNT          828 a         1134 c          991 g          839 t
ORIGIN      
        1 cacgccggcc attcttgcgg cgttgcgcac cctcagctcc ggctgcggga atcagaagca
       61 gaaacgcgag taagagaatc ttcaagaagc tcataaacga atcgctattg agaccaggag
      121 agatgatgac cgtccgacgt ctgaactcgg attagagcgc acaacgccta aaagagttcg
      181 ccgtcgcgga tagcgaaatc cgtgggacac tcaatttgga tgaatttaac gatagcggat
      241 gatgctgcgt cgtcgtcgct taactggagt ccggatcgaa agcagactct tgtggcgacc
      301 gttcagaatg aacgccagcc cctgatgcgt gattcccagt aggcgcgccg cgcgcgtcac
      361 actgccccct gaagtttcca gggcttggcg aatcagctca ccttcgtagt tgagaaccag
      421 ttcatccaac gaaactccgg gagttagtgt tgcggagcgt aactccgacc gtgcggcgct
      481 cagcttttgg ccgttgggag aatccgcgga gggcttatcg ctgaaatgtt tctcgcgggt
      541 aagaatctgg cgagcacatt ctccaaggcg cgtccgcact cccaggtgct gcgagcgggc
      601 caataatgat tccgcctgca ggtagtactc gtggaggtcg gagttgggaa caaactgagg
      661 cagttcttcg acgatcgtga gcgctgcaat accagctgag tctggatcac cggcctgctg
      721 agccacggtc atccctcgct tcaatgccgc gagcgcggca tcgctggaat taatacgagc
      781 tagagatgtc ccataggtaa tcagggcgcc ggccagtaat gactgctccc caccttgttc
      841 caacgaccgc acgctgctgc gcgaaatagc ctccgcctcg gcgtatcgtt tctcaccgag
      901 aaaagccttg gcgcgcgaat cgtcaaactg agcaaccagg ccctgatctt tcaacgccac
      961 ggccacggct cgagcgcgtc ctaagtgctc gtgcgcatcc tcatagcgcc ccaaatggac
     1021 aaacagaaat ccgagattgt tttcgacgac tccaaggaac cgctggtgac ccgcctcttc
     1081 aaaatgaaaa ccggcagcag cgtattcgac caaggcccga tcgatgtaat cttcgcgatg
     1141 ctctgcgagg ccaatattct taaggacggt cgcaaactcg ttgtggaact ttccccttaa
     1201 cgcatgatta ctgctctttt caaacaacgg cgccgccgac tggtgaattc gcaaggcatc
     1261 acgatagcga actgagactt tttccacgac cgcagcattc agaagcgcgc gcaagcgttg
     1321 ctctgtttca cgctccccca gctgcgccaa cacttcctga aaagtgacac gcgcttcatc
     1381 aagagcgcct tcgcgccagt agcagatgcc cagatcaatg cgcgcctcgg cggcacggtc
     1441 atcaagtccc agcctttcga acagcccagc gctctcggag ataagatcct tcgctgtttc
     1501 ctgcgcgccg gggatctgtt gggcgctgcc gatccagccg gttaaggtgc caactcgcaa
     1561 aaggacttcg gcgctcgcgg catcatccag cccccgtaat actggccgtt ctccgatgcg
     1621 ctgccaaaaa ccatgcagca gttgacgcgc ggcttcgaaa ttacccgcct cttcctgctc
     1681 cctggcctgt gcacactgaa ggcgagcctc gtcgcttaag ccgggttcga agctgcctat
     1741 ttcacttacc gtcgatttat gtaattgcat gtgaggtaac ccttttcaag atttagcaag
     1801 ttcccttgca cgagatttat tacgcggtta ttatgcgctc cgctaatgac ctccagttag
     1861 cgcttcccaa cattcgcgcc gaactggagg atgccaaccc gtagccaaca acttaggaga
     1921 ccaacgatga agaacgttaa agcaatgttc ggaacaataa ctctcggcct gctgctgagc
     1981 agcgtcgcac ttgcgggcga tgttagtacc ccgggttatg taccaccacc gccaccgcca
     2041 gccgcggcgc cattgcaaga aacgggcgac tcagacataa gctcgtcaag cctcgctatc
     2101 gactcgtcaa gcctcgctat cgaccttctg attgcggcgc tcggggcttt ctattaaaag
     2161 atcgcaacta caggtcaccg ccacgccctc taattgacct tccgctgcaa gtcgtgcggc
     2221 ctagcttttg tatttcaaag acttgccgga gatgtcccaa ttgctggatc agggagcgtt
     2281 ggcgcgtgaa tcctaacgcc ttttcccacg tttacgcaat agggacttgc ctctcccggc
     2341 agtatttctc tgccggaagg acgctgctcg cattgtttcc gaggggacac ctccgggcgg
     2401 gaaccagtgc cgttcattgc gtgtttgctg cgacaagtcg ctggacggtc tccagagtat
     2461 cgatctcttc cggcgtcttg tccgtcctta gccggagaat tcgcggaaat cgcagcgcat
     2521 aaccgctctt gtgtcgccgc gattcctgaa ccctgtcaaa ggtgacttca attacgattc
     2581 gcggctctac cagacgaacc cgaccgtggg caaactcctt gaccgtatga gccttaaacc
     2641 actcagtcaa ctcggttaat tctacgtccg taagccccga ataggctttt ccgatattta
     2701 gaagttcgtc gtcctcgagt gagcggcgaa ttgcaaaggt atagtccgat agcagattcc
     2761 ttcgcttgcc gtgtccaacc tctaccgcgg tcactaccac gtcaagcgtc gcaatagcgc
     2821 gctttacctt gagccactct cgcccgcggc gacctggttt gtagactgag gctggactct
     2881 tgaccatcaa gccttcgttc ccacgggctc gggccgctgc aaattcgtcg tccagggcgg
     2941 ctacttcctg gaagcgcttc gcaagcgaca gacgcagagt gccttcactg ggaatcagct
     3001 cagcaagagt ctttagtcgt tcttccagga cctcgttaat cagtacgcgg ccgctggcgt
     3061 agagcaggtc ataagtgacc agcacgactg ggacatccgc gagcagatcg gcgccaatcg
     3121 tttttcgacc cagtcgtttc tgaagctcac tgaacggcag gatgcggtcg ccggtcgccg
     3181 gcacgatttc gccgtcgatt actacatcgg tcgctaagtt ctttagtggt tctactaact
     3241 ccgggaaccg atgggtaatc tcatccatcg tgcgcgaata gatcgagacc cgtccttcct
     3301 taacgtgagc ctgtgcacgg atgccgtcaa acttatcctc aacaaagaac tcctcaggca
     3361 tggtgcgcgc gatgtctgaa agatccgcgg cgggagtggc gagcataaac ttgatgggat
     3421 gaaagagacg catctcgatg ttctcgagat cacccgatct agtccgaacc gcagcctcac
     3481 caatgtcgcc aagcaacatg ttcgcctggg ccactgctgc caaaggctgt ccgaaagcac
     3541 gcgccgtagc atcttcgacc agaccttctt tcaatccgat gcgaagatcc ccaaccaaca
     3601 acttcacgag atacttggct tcgagcggag ttgcctcgcg taaaacagtg gcgagcaacg
     3661 cggtcttgtt cttggtgcca cgcgtctcac taagacgtgt gatgagcgat tccgtttctg
     3721 ccaacgtaat tctgggttga ttagcggcgt gccgctcctg tacggcttcg aaagcgactt
     3781 caccggcgtc gc
//
LOCUS       DASUEX010000362         2510 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1442348, whole genome shotgun sequence.
ACCESSION   DASUEX010000362 DASUEX010000000
VERSION     DASUEX010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1442348"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..825)
                     /locus_tag="VFV61_06055"
     CDS             complement(<1..825)
                     /locus_tag="VFV61_06055"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX4900077.1"
                     /translation="MITRRKFLTSSALFGAALFVRPSDLFTNAASLSLAEPLLTARRG
                     ETLITFLHTNDTHSQIDPLPPNDRLYPGKGGVARRATLVKRIRRQNPNTLLIDAGDVF
                     QGTPYFNFYRGEVEYKAMSAIGYDVGTLGNHEFDNGVDALAAALKFANFDLVSANYDV
                     RGTALESKVKRYVVRTVGGVRVGLFGMGISPVGLITPANFKGVTYQDPVAASREVVSI
                     LREKERCTLVVGMSHLGYYPEPKPGEIGDSQVAAQVDGIDFIASGHTHTFMAEPVNV"
     gene            complement(822..1670)
                     /locus_tag="VFV61_06060"
     CDS             complement(822..1670)
                     /locus_tag="VFV61_06060"
                     /inference="COORDINATES: protein motif:HMM:NF014871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX4900078.1"
                     /translation="MNTRLRYAQRFLLLLCGVIALTATPCYAQQKSRTQTSPCRQSPC
                     ITRTGQTIVDAKIPDDAAIERLLKPYSGKVRALNVVIGKLDGELRKGGVGAGSLGNFV
                     TDGIRSQASAKLGRPVLLAVTNSGGLRKNTIAAGELRASDIFELMPFENALIQVDLSG
                     EDLLKLLAVILDSRDAQSGAKIHYRMNADKAELVSAKFLDSRGQETEIDPRAKYSIVT
                     IDYLLNLASGRYSVLQRGKNPTPLGITLRDALMNYVKAETAAGRPIHATLDTRFVNDT
                     PEGQPQ"
     gene            1805..2371
                     /gene="hpt"
                     /locus_tag="VFV61_06065"
     CDS             1805..2371
                     /gene="hpt"
                     /locus_tag="VFV61_06065"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEX4900079.1"
                     /translation="MATSSAQLQSEFTNPNLEVLLSEQRIQDRVKELGARITQDYAGR
                     NPLLIGVLKGACFFLTDLMRAIDTRVGIEFMAISSYGASTRTSGEVRILKDLDVPVEG
                     RDLIVVEDIVDTGLTLSYLLANLKSRGAASVKLVALLDKFERREREVEIDYLGFQIPD
                     HFVVGYGLDYAERYRNLPFIAVLKNPEA"
BASE COUNT          589 a          673 c          660 g          588 t
ORIGIN      
        1 tacgtttacc ggctctgcca tgaaggtgtg agtgtgtccg ctggcaataa aatcaatgcc
       61 atccacctgg gcggccacct gagaatcgcc gatctcgcct ggtttcggct ccgggtagta
      121 gcccaggtgc gacataccga caaccaatgt acatcgctcc ttttctcgca gtattgacac
      181 gacttcacgg gacgctgcca ctggatcctg ataagtgaca cccttgaaat tcgccggggt
      241 gattaagccc accggactaa tccccatgcc aaacaaaccg acgcgcacac cgccaacagt
      301 acgcacgacg taacgcttca ccttactttc aagcgcggtg ccccgcacat catagtttgc
      361 ggaaaccaag tcgaagttgg cgaacttgag cgcggccgcc agcgcgtcga ctccgttgtc
      421 gaattcatga ttgcctaaag ttccgacgtc gtaaccgatc gccgacatcg ctttgtattc
      481 gacttcaccg cgatagaaat tgaaataagg cgtgccctgg aaaacatcac cggcgtcgat
      541 cagaagtgtg tttgggtttt ggcggcgaat ccgcttcacg agagttgcgc gacgcgcgac
      601 accgcccttg cctggataga gacggtcgtt cggcggcaat ggatcgatct gtgaatgcgt
      661 gtcgtttgta tgcagaaaag taatcagagt ttcgccccgg cgcgccgtca agagtggttc
      721 agcgagagac aggctcgccg cgttcgtaaa caaatcggaa ggacgaacga aaagagcggc
      781 accaaaaaga gcgcttgaag tcagaaattt tcggcgcgta atcattgtgg ctggccctcc
      841 ggggtatcgt ttacaaagcg tgtatcgagg gtcgcatgaa tcggccgtcc ggcggcagtt
      901 tcggccttta cgtaattcat cagcgcgtcg cgcaaagtaa tccccaaagg cgtgggattc
      961 ttgccgcgtt gcaagactga gtatctgcca ctggcaaggt tcaagagata atctatcgtg
     1021 acgatcgaat acttggccct gggatcgatt tcagtttctt gcccacggct atcgaggaat
     1081 ttggcgctga ccagctccgc cttgtcggca ttcatccggt agtgaatctt cgcgcccgac
     1141 tgtgcatctc gcgaatcgag aatcacggcc agcagtttca ataggtcttc gccagagaga
     1201 tcaacttgaa tcagcgcgtt ttcgaaaggc atcaactcga atatgtcgct cgctcgtaac
     1261 tctcccgcag cgatcgtgtt ctttcgtaac ccgccgctgt tggtaacggc aagcaacaca
     1321 ggcctgccca gcttggcact tgcctggctg cgaattccgt cggtaacgaa atttcccagc
     1381 gagccagcgc ccacaccgcc tttccgcaac tccccatcaa gcttcccaat tacaacattg
     1441 agggctcgca ctttcccgct gtagggcttg agcagccgtt caattgcggc atcatcggga
     1501 attttagcgt caacaatcgt ttgaccggtg cgcgtgatgc agggcgattg gcgacaggga
     1561 ctggtttggg tacggctttt ctgttgcgcg tagcagggtg tagccgtgag cgcaataaca
     1621 ccacagagca gcaaaagaaa gcgctgcgca taacgcaaac gagtattcat agttcctgtt
     1681 ttatttgcat tgaacgttga cgttaaggga atagcttgca tagtatgtcg caccacaagc
     1741 ttcatggcaa acagttagtt cgcgcgctaa aaccccttca attgcactaa aagaggagag
     1801 catcttggcc acttcatctg cacaacttca atcggaattc accaatccca atctcgaggt
     1861 actgctttcc gagcaacgga ttcaggatcg tgttaaagag ttgggcgcca ggatcactca
     1921 ggattatgcg ggacgaaatc cgctgctgat tggcgtcctt aaaggcgcct gcttcttcct
     1981 aacagacttg atgcgcgcga ttgatacgag ggtgggaata gagttcatgg caatctccag
     2041 ctacggtgcc tcgacacgga cttcggggga ggtacgcatt ctcaaggatc tcgacgtccc
     2101 ggtcgaagga cgtgatctga tcgtggttga agatattgtc gacaccggat tgaccctttc
     2161 ttacctgctg gccaatttaa agtctcgtgg cgcggcgagc gtgaaactgg tggcgttgct
     2221 cgacaaattt gagcgacgcg agcgcgaggt ggagatcgat tatctgggct ttcagattcc
     2281 ggaccacttc gtagtcggtt acggactcga ctatgcggaa cgctaccgca acctgccgtt
     2341 cattgccgta ctaaagaatc cggaagcata aaatccggtt tcgaactctg aatcgttcag
     2401 ccgttaacag aaaaacgatc cactaaatca cactgaacaa caccaacttc ttcgggtgat
     2461 ttctaggggt tttcggatcg tttttgactc attgtcgtcg atcaactcta
//
LOCUS       DASUEX010000363         3337 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_394570, whole genome shotgun sequence.
ACCESSION   DASUEX010000363 DASUEX010000000
VERSION     DASUEX010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3337
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394570"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="VFV61_06070"
     CDS             <1..324
                     /locus_tag="VFV61_06070"
                     /inference="COORDINATES: protein motif:HMM:NF015922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HEX4900080.1"
                     /translation="VSELGYGKQTPITTYRLQSRGGRGVINMKTTEKTGKVVAVFPVE
                     EDSEIMIITQQAKLIRIEANQIRKTGRSAQGVRLIKTDVGDKVTSASLVEAAAEEETE
                     ETPAS"
     gene            complement(470..1228)
                     /locus_tag="VFV61_06075"
     CDS             complement(470..1228)
                     /locus_tag="VFV61_06075"
                     /inference="COORDINATES: protein motif:HMM:NF014448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HEX4900081.1"
                     /translation="MSGPAPESIVVRVLFFGAARDAAGQSEATLSLHPSTNAASAFAQ
                     VVAAFPALDRFGRSLLFAINEEYAQADREVRSGDELAVFPPVSGGSDEAQTTQPATRE
                     VSDFFELTTAPINVGDVARRVVLPECGATVTLDGYAREWTNGRRTLHLVYEAYEPMAL
                     NEMRRLGREAHEKFQIAYLGIVHRTGKLEIGDTSVVISVGAPHRRAAFEACEWAIREL
                     KRTVPIWKKEVFADGEVWVEGEGGKQTFEETRDG"
     gene            complement(1231..1932)
                     /locus_tag="VFV61_06080"
     CDS             complement(1231..1932)
                     /locus_tag="VFV61_06080"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEX4900082.1"
                     /translation="MSEELDRVKQVRELPLFPLPIVLFPGVPLPLHIFEPRYRQLLTD
                     VRKDNSLFGLSYFDPGSSTSERPLPGHIGCVAEISETQTMPDGRSNILTVGLVRYRAD
                     DYVDRGDPYLVARVSFFEDDEEDESLLGESSREVAQIFTRIARAVRLINDERANLPDI
                     SDTDPQRLSFLVAAAMEVDTDVKQELLELRLTSERLRRLRDMLARAVTGYEERARVHE
                     IAKGNGHSGRKIEIE"
     gene            complement(2033..2452)
                     /locus_tag="VFV61_06085"
     CDS             complement(2033..2452)
                     /locus_tag="VFV61_06085"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX4900083.1"
                     /translation="MKPRILIYDRDQAITRQLFWTLCEEYDVVTANDLHTAMRRASAY
                     EPAIAVLDLNSPAAPGYDDAGLRILQYLRSHFPKSRILGMTSEIFLDKKKKYLSLGVD
                     ELLDKPFDTQQLLDFLRRLAPLRSLDGIESGSFTLRY"
     gene            complement(2820..>3337)
                     /locus_tag="VFV61_06090"
     CDS             complement(2820..>3337)
                     /locus_tag="VFV61_06090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900084.1"
                     /translation="KYVGGASSSGVEYISALADLFRPDSQTTFKPVDTDLLREHRTVV
                     FEYEVKRPNSKLTLTADRTRTTDVGSRGRLWIDRENNRVLRFEQIATEIPSDFPITAA
                     SSLIDYDWVTIGEGKFVLPVRSEILLTTVNKGQSLQSRNEIRFRGYQKFGAELKVIDE
                     IDESDFPPEKP"
BASE COUNT          784 a          909 c          912 g          732 t
ORIGIN      
        1 gtttccgagc tgggttatgg gaagcagaca ccgatcacta cttaccggtt gcaatcgcgt
       61 ggtggcagag gcgtcatcaa catgaagacg actgagaaaa ccggaaaagt tgtagcggtg
      121 tttccggttg aggaagactc ggagatcatg atcattacgc agcaggctaa gctgatccgc
      181 atcgaggcta accagattcg gaaaaccgga cgcagcgctc agggtgttcg tttgatcaaa
      241 actgacgttg gcgacaaggt caccagtgct tccctcgtcg aggctgccgc tgaggaagag
      301 acagaggaga cgccagcaag ttaaatcatt ggcaattggc aattgccaat tacccgattg
      361 cgactggtgc ccgaacttga tacagcaatt gcaccattaa aaaatcagct ggttcttcgt
      421 tgatacgcac aaactgcttg taccgacgcc ggctaattgg caatcgccgt cagccgtccc
      481 gggtttcctc aaaagtttgt ttgccgcctt ctccctcgac ccaaacctcg ccatcagcaa
      541 acacttcctt tttccaaatc ggcaccgtgc gcttaagttc gcgaatcgcc cactcgcacg
      601 cttcgaaggc ggccctccgg tgcggcgctc ctactgaaat caccacactg gtatcgccga
      661 tttcgagttt gccggtgcgg tggacaatac caaggtaggc aatctgaaac ttctcgtggg
      721 cctcacgtcc cagccggcgc atctcgttga gcgccatcgg ctcatacgct tcgtaaacaa
      781 ggtgtagcgt gcgacgtcca tttgtccact cacgagcgta gccatcaagc gttaccgtgg
      841 cgccacactc aggcagcacc acccgtcgcg ccacgtcgcc aacgtttatt ggtgcagttg
      901 ttagctcaaa aaagtcggac acttcgcggg ttgccggctg agttgtttgc gcttcatcgg
      961 aaccgccgct cactggagga aagacggcaa gctcatctcc tgatctcact tcacgatccg
     1021 cctgagcgta ttcttcatta atcgcgaaca ggagagaccg cccaaagcgg tctagcgccg
     1081 gaaaggccgc cacaacttgt gcgaaagcac ttgcggcgtt agtggaaggg tgtaatgaga
     1141 gcgtggcttc agactgcccg gccgcgtcgc gagcggcgcc aaagaaaagt acccgaacaa
     1201 ctattgactc cggggccggg cctgacatgt tcactcgatt tcgattttcc tgccgctgtg
     1261 gccgttacct ttggcgatct catgaacccg agcccgctct tcgtaaccgg tgacggcgcg
     1321 cgccagcata tcgcgcaacc tgcgcaagcg ctcggaagtt aggcgcagct ccaacagctc
     1381 ctgctttaca tcggtatcaa cttccatggc cgctgccacc agaaatgaaa gtcgctgggg
     1441 atcggtgtcg gaaatgtccg gcaaattagc gcgttcatca tttatcagac ggactgcccg
     1501 cgcgattcgc gtaaaaatct gtgcgacctc gcgggagctc tcacccaaca gtgactcgtc
     1561 ttcctcgtcg tcttcaaaga agctgacgcg cgcgacgagg tacggatcgc cgcggtcaac
     1621 gtaatcatcc gcacgatatc taacgagtcc gacagtgaga atatttgaac ggccgtcggg
     1681 cattgtttgc gtctcgctga tttcagctac acaaccaata tgcccgggaa gcggtcgttc
     1741 cgaggtcgac gagccaggat cgaagtatga aagcccaaag agactgttgt ccttgcgaac
     1801 gtcagtcaac agttgccggt agcggggttc aaagatatgc agcggcaagg gcacaccagg
     1861 gaacagcacg atgggcaacg ggaaaagcgg taattcgcgc acttgcttta ctcgatccag
     1921 ttcttcgctc atgagagaaa aacacaccgg ccggtgtcgg caatacttgt cagccctaaa
     1981 aactagatgg cgtcttgcgg ttcccggatt atcgcagaca gcggaagaga agtcagtagc
     2041 ggagtgtgaa ggagcctgac tcgataccgt cgagcgaacg caaaggcgcg aggcggcgca
     2101 gaaagtcgag caactgttgc gtgtcgaacg gtttgtcgag cagttcatcg accccgaggc
     2161 ttaaatactt ctttttcttg tcgagaaaga tctcggaggt catgcccagg atgcgcgact
     2221 tggggaagtg acttcttaga tactgaagga ttcgcaaacc ggcgtcgtcg taaccaggag
     2281 ccgcaggtga gttcagatcc aacacggcaa tggccggttc atacgcggag gcgcgccgca
     2341 tcgccgtatg caaatcattt gcggtgacaa catcgtattc ttcgcacagt gtccagaaca
     2401 gttgccgagt gatcgcttga tccctgtcgt aaatcagaat ccgcggtttc aaggcgaggc
     2461 ctccgagctt gcttatcgca atgagtgttc cgttcgctga tgtaagtcaa agatttttcg
     2521 tgagactagg cggggatttg aaaaagagga agtgcaggga accttccctg tcagctttcg
     2581 gagaccatca ggatgaagtt gtctgcaaaa gtagacgcct ttcgattgcc gattgcggat
     2641 tgccgattgg cttgcagcca gagaagcaat cgactgcctt gagtgttacc tgacaagttg
     2701 agcggaacgg cactgtcgaa gccccaccgg ctacccgaca tgtcgggatc cgcctctctc
     2761 cgctgcgggc aaatcatcaa tcgccaattg gcaattggca attggcaatc gccaatgatc
     2821 tatggctttt cgggtggaaa gtcgctttcg tcgatctcgt cgatgacttt taactcagca
     2881 ccaaactttt ggtagccacg aaaccggatt tcattgcgac tctgaaggga ttgacccttg
     2941 ttcaccgtgg ttagcagaat ttcggagcga accggtagca caaacttgcc ttcgccaatg
     3001 gtcacccaat cgtaatcgat caagctgctc gccgcggtga tcggaaaatc ggaagggatc
     3061 tccgtggcaa tctgttcaaa acgcagcacg cgattgtttt cccgatcgat ccacagacga
     3121 ccgcgtgagc cgacatcggt tgtacgcgtg cgatccgccg tgagagtcag tttcgaatta
     3181 ggccgtttga cttcgtattc aaagacaacc gtgcggtgct cgcgcaacag atcggtatcg
     3241 accggcttga aagttgtttg tgagtctggt cgaaagagat cagctaacgc ggaaatatac
     3301 tcgacaccgc tggaactggc gcctccgacg tacttac
//
LOCUS       DASUEX010000364         3835 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1105658, whole genome shotgun sequence.
ACCESSION   DASUEX010000364 DASUEX010000000
VERSION     DASUEX010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3835
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1105658"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1709)
                     /gene="uvrB"
                     /locus_tag="VFV61_06095"
     CDS             complement(<1..1709)
                     /gene="uvrB"
                     /locus_tag="VFV61_06095"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEX4900085.1"
                     /translation="MRFKLSSEYEPKGDQPQAIDAIVRGLQDGAKDQVLLGITGSGKT
                     FSVASVIERTQRPALVLAHNKTLAAQLYQEFKSLFPENAVEYFVSYYDYYQPEAYVPA
                     SDVYIEKEATINDEIDRLRMSATRALFERRDVIVVASVSCIYGLGDPDAYYGMLLFLE
                     PGTRISRESLLQKLVELQYERSDINFQRGTFRVRGDVVEVFPSYQDQALRIELWGEEI
                     DSISTIDPLLGEVLQKHTSRLPIYPKTHYVMPGSTIKRALKSIREELDWWEPKLIAEG
                     KLVEAQRLHQRTMFDLELIKEMGFCRGIENYSRHLTGKKPGQPPPTLLDYVPRDTIMV
                     IDESHQSVPQVRGMFEGDQSRKRTLVEYGFRLPSALDNRPLNFAEFEARIGQTIYVSA
                     TPGPYELTKSGGEVIEQIIRPTGLLDPEVEIHPVKGQIDHLLEECRLRAERNERVLVT
                     TLTKRMAEDLTEYFTEVDLRVRYLHSDIETLERIKILRDLRRGEFDVLVGINLLREGL
                     DLPEVSLVAILDADKEGFLRSERSLIQTIGRAARNSGGKAILYADRITDSMRKAMEET
                     ERRR"
     gene            complement(1758..2504)
                     /locus_tag="VFV61_06100"
     CDS             complement(1758..2504)
                     /locus_tag="VFV61_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900086.1"
                     /translation="MPKNFLTSNRKDPTLEPVKISNVAAPIATDEGPRREADDRGRRD
                     DDRWRKEQETLRRAAVELAKRRAQEEVAAMSQLTTVAEANSLETITEFLEDPSREVRN
                     QAARELYRVNPEFAATFFNSALREGPPARRRTIGAALAGSGLVGDAIQALNSGRQKNA
                     YSTISLLFLVAKAGEVQPLLSLLENHPSIELRLALIELLALSGETAILPALKRLAVKG
                     VLPAEVRSAVMEAIYQLTTQSRDAQLEVSS"
     gene            complement(2652..>3835)
                     /locus_tag="VFV61_06105"
     CDS             complement(2652..>3835)
                     /locus_tag="VFV61_06105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900087.1"
                     /translation="LTEGEDGEVPQWEQGKPTVPEGFKQFVAKQDTDSLFTSLRKETM
                     VKRIRATSILENPSARFFLRRAKDAHVEGYASKLLTGDVSPADTFSISRLEDAGLVER
                     EVQVSCRKTGHALFRLPTANALAVVTVSDATCSECGSPVADEKVEEVIAPTVLAESLL
                     EDGSWLISRLHHILREMGISESEIAVGPAEGEGYGQMMSNICGESFLIVARDGELTPA
                     FARWAIDLEVDTQASHLVVVATDRIHNQAAVLLHNHARHRVRAGQNFELILADEAAVA
                     GQELSQAFERVSQRVIAEQLCELDSALGLNVTQLVVAKFRMLSTTDEDGEESFLNVST
                     PDVTQLRPPLSLAASASANGGDVFDVEGYQVVDPSDNISANEGPGNGSLSSNQASETT
                     V"
BASE COUNT          872 a         1088 c          994 g          881 t
ORIGIN      
        1 cggcggcgct cggtttcttc catcgctttg cgcatcgaat cggtgatccg gtcggcgtag
       61 aggatcgctt tgccgcccga atttcgcgcc gctcgcccga tggtttgaat gagcgagcgt
      121 tccgaacgca gaaacccttc cttgtcggca tcaagaatcg ccactaagga aacttctggt
      181 aaatccaggc cctcgcgcag gaggttaatg ccgaccagga catcaaactc accgcgtcgc
      241 aggtcgcgca gaatcttgat tcgctcaagc gtttcgatat cggaatgaag ataacgaacg
      301 cgcagatcca cctcggtgaa atactcggta agatcctcgg ccattctctt cgtcaacgtt
      361 gttaccaaaa cacgttcgtt gcgttctgcg cgcagccggc attcctccag caaatgatcg
      421 atttgacctt tcacgggatg gatttctact tccggatcga gtagacccgt aggcctgatg
      481 atttgttcga tgacttcccc gcctgacttc gttaactcat acggcccggg ggtcgctgaa
      541 acgtagattg tctggccaat ccgggcctca aactcagcga agttcaaagg acgattatcc
      601 agcgcggagg gcaggcgaaa tccatattca acgagcgtgc gcttgcgcga ctgatcgcct
      661 tcaaacatgc cgcgtacctg gggcacggat tgatgcgatt catcgatcac catgatcgta
      721 tcgcggggga cgtaatcgag cagcgtcggg ggcggctgtc ccggcttctt cccagttagg
      781 tgtcgggagt agttctcgat accgcggcaa aaacccatct ccttgatcag ttctagatca
      841 aacatggtgc gctgatgaag gcgttgcgct tcgacaagct tcccttccgc aatcaacttt
      901 ggctcccacc agtccaattc ttcgcgaata gacttaaggg cacgcttgat agtgctgccc
      961 ggcatcacgt aatgagtttt gggatagatg ggaagccgtg aagtatgttt ctgcaaaact
     1021 tcgccaagca aaggatcgat ggttgaaatc gagtcgatct cttcgcccca aagctcgatg
     1081 cgaagcgcct gatcctgata actcggaaac acttcgacta catcgccgcg cacccgaaac
     1141 gtcccccgtt ggaagttaat gtcggaccgt tcgtattgca attctactaa tttctgcagc
     1201 agcgactcgc gagagattcg cgtccccggt tcgagaaaaa gcagcatgcc gtagtaagcg
     1261 tcaggatcgc ccaaaccata aatacaggag acactggcca ccacaatcac atcgcggcgt
     1321 tcgaaaagcg cgcgcgtggc agacattctt agacgatcaa tttcatcatt aatcgtcgct
     1381 tctttttcaa tgtagacgtc agaggcggga acgtatgctt ctggctgata gtagtcgtaa
     1441 taagaaacaa aatactcgac ggcgttttcc ggaaagagcg atttgaattc ctggtagagc
     1501 tgggcagcga gggtcttgtt gtgagccaga acaagagctg gtctttgggt ccgctcaatt
     1561 acacttgcga cggaaaaggt tttgccacta ccggtgatgc cgagcagcac ctggtctttc
     1621 gccccatctt gcagaccacg aacgatagcg tcgatagctt gaggctgatc gcctttcggc
     1681 tcatattccg aactgagttt gaaacgcatt gattcacgaa tagtaaatcg tgaatcgtaa
     1741 aatcgtgaaa tgagcagtca actcgatact tcaagttggg catcacgaga ttgcgtggtg
     1801 agttgataaa tggcttccat tacggccgaa cggacctctg caggcaggac tccttttact
     1861 gccagacgct taagagctgg caaaatggca gtttcaccac tcaaagcaag aagctcaatc
     1921 aaggccaagc gcaactcgat actgggatga ttctccagca aactcaatag cggctgcacc
     1981 tctcccgcct tcgccaccag gaacagcaat gaaattgtgc tgtatgcgtt cttctgcctg
     2041 ccactgttca gtgcttgaat cgcgtcaccc accagccctg agccagctaa cgcggcgcca
     2101 atcgtgcggc gccgtgccgg cggaccttca cgcaacgcgc tattgaagaa agtagctgcg
     2161 aattcggggt tgactcgata aagttcacgg gcggcttgat tgcggacttc gcgcgaaggg
     2221 tcttccagga attcggtaat cgtctccagt gaattcgcct cggcgacagt ggtcagttgc
     2281 gacatcgccg cgacttcctc ttgtgctcgt cgtttcgcca gttctactgc ggcgcggcgc
     2341 agggtttctt gttccttgcg ccagcgatcg tcatcacggc gcccgcgatc atcggcctcg
     2401 cgccgcgggc cctcgtccgt ggcgatcgga gccgccacgt tggatatttt tactggctcg
     2461 agtgttgggt ccttacgatt gctggtgagg aagttcttcg gcatttagat ttccccgcca
     2521 cagacgattg gggtaaagcc gcttgagagg gatagctggg taagatgctg agccacttcg
     2581 cagcaagcga tcacgaatta tggtgacgct caatcctcct ggagctgtca ctcgcgggca
     2641 aaagaaaccc gtcagacagt cgtttcgctg gcttgattgg aacttagcga cccgttcccc
     2701 ggaccttcgt tggcactgat gttatcgctc gggtcgacta cttggtatcc ctcgacatca
     2761 aaaacgtctc caccgttcgc ggaggcgctc gcagccagac tcaaaggagg tcgcaactgc
     2821 gtaacgtccg gcgtggacac attcaaaaaa ctttcctcgc catcctcgtc agtggtggaa
     2881 agcattctga atttagcgac gaccaattga gtgacattca aaccaagtgc gctgtccagc
     2941 tcgcaaagct gctccgcaat aactcgctgc gaaactctct caaacgcctg gctgagctcc
     3001 tgccccgcca ctgcggcttc atcggcgagg atcaattcga agttctgccc ggcccgaacc
     3061 cggtgtcggg cgtggttgtg aagcagtacg gcagcctgat tgtgaattcg atcagtagcc
     3121 acaacgacga gatgcgaagc ttgtgtatca acctccaaat cgatggccca gcgtgcaaag
     3181 gccggagtca attctccatc acgagccaca atcaggaacg actcgccgca aatattggac
     3241 atcatctgcc catagccttc gccctctgcc ggtcccaccg cgatctcgct ttcggaaata
     3301 cccatttcgc gaagaatgtg gtgcagacgg ctaatcagcc acgagccatc ttcgagcagc
     3361 gactccgcca acactgtcgg cgcaatcact tcttcaacct tctcatcggc aacgggcgaa
     3421 ccacactcgc tgcaggtagc atcactcacc gtcacaaccg ccagggcatt tgcagtcggc
     3481 agacgaaaga gcgcgtggcc cgtcttgcga cagctaacct gcacctcgcg ttctaccaat
     3541 ccggcgtctt caagtctgct gattgaaaac gtatcagcag gcgaaacgtc tcccgtgagc
     3601 agtttcgagg catacccctc aacgtgagcg tccttggcgc gtcgcaaaaa gaaccgtgcg
     3661 ctcggattct ctagaattga ggtcgcgcga atgcgcttga ccatcgtttc cttacgcaaa
     3721 ctggtaaaca acgaatccgt atcctgttta gctacgaatt gtttaaagcc ctcggggaca
     3781 gttggcttac cctgttccca ttggggaact tcaccgtctt ctccttcagt taggt
//
LOCUS       DASUEX010000365         2859 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1442865, whole genome shotgun sequence.
ACCESSION   DASUEX010000365 DASUEX010000000
VERSION     DASUEX010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2859
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1442865"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..593
                     /locus_tag="VFV61_06110"
     CDS             <1..593
                     /locus_tag="VFV61_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900088.1"
                     /translation="DRKVLGIHIWSQGKIYDPEGLLSTLPAIATTLLGVISGELMRSQ
                     RRQYEKVSLLFIAGISCIIGGWLWSLFFPINKSLWTSSYVLFTGGLAMQFLALCYWLI
                     DMKGYRRWAKPFVVFGVNAITLFVGSGLMARLMGLIKVPRADGTKIALKTWIYDGLFL
                     SWAPPYQASLSFAIAFILLWLGLMWILYKRKIFIKV"
     gene            852..1142
                     /locus_tag="VFV61_06115"
     CDS             852..1142
                     /locus_tag="VFV61_06115"
                     /inference="COORDINATES: protein motif:HMM:NF020317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HEX4900089.1"
                     /translation="MPLYISLVRWTQQGVAHVRDSQNRLDSGRKAFREIGVEIKQVHM
                     TMGRYDLIMLLEAPTDDAVATALLSLASQGNVQTETLKAWTEDEYRKITGSI"
     gene            complement(1254..>2859)
                     /locus_tag="VFV61_06120"
     CDS             complement(1254..>2859)
                     /locus_tag="VFV61_06120"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HEX4900090.1"
                     /translation="DLLIKDGQIVDGSGRPAYRADLAVTGDRIVRIGNLKSATAKRVI
                     EARDLVVAPGFIDMLGQSETYVLIDPRVMSKVMMGVTTEITGEGESIAPINERLIKEQ
                     ADFFQRFDLKVDWRTLADYFRRLEKQGAGVNLGTFVGATQVRAHVMGFDNRPPTAAEL
                     AEMKQLVADAMKDGALGLSTSLQYVPARFAKTDEIVELAKVAQQHGGIYATHQRSEAN
                     ALDESLQEVFTIARQANIPVEIWHLKTAYQKNWGRMPEVLKKISAARAQGLDITADVY
                     PYIAGSTSLSACLPPWAQEGGGEKMLSRLRDPELRQRLKKEISADSKDWENIYLGSGG
                     AAGILIGSVVNRELESLQGKRLEEIATAQNKDPLDALLDLILADHGQTGAIYFMMSEN
                     DLLAAMKSPFVSFCTDSGARATDGPLSGAKSHPRGWGSYPRILGRYVREQRVLPLEKA
                     VAKMTGMPAKRVGLKDRGLLREGMFADIAIFNPQTVIDRATFEAPNQYPEGVLYVIVN
                     GQLSVDQGKRTAALAGRPLRGPGFSR"
BASE COUNT          630 a          762 c          778 g          689 t
ORIGIN      
        1 tcgatcgcaa agtcttgggc attcacatct ggtcgcaagg gaagatttac gatccagaag
       61 gacttcttag cactctcccg gcaatcgcca cgacactcct gggagtaatt agcggtgagc
      121 taatgcgctc gcaaagaagg cagtatgaga aggtgtcgct gctgtttatc gcgggcatca
      181 gttgcataat tggcggctgg ctttggagtt tgttctttcc cattaacaag tctctgtgga
      241 ctagttcgta cgtcttgttc acaggcggac tagcaatgca gtttctggcg ctctgttact
      301 ggctgatcga catgaaaggg taccggcgct gggcgaagcc atttgtcgtt ttcggcgtga
      361 acgcgatcac gctgtttgtg gggtctggtt tgatggcgag gctgatgggg ttgatcaaag
      421 tgccgcgcgc ggacggaacg aagatcgccc tgaagacgtg gatctacgat gggctcttcc
      481 tttcctgggc gccgccatat caagcttcgc tatcgtttgc gatcgctttt attcttctgt
      541 ggctgggatt gatgtggatt ctttacaagc gaaagatttt cattaaggta tgaacaaagg
      601 gcgaaagtcc aaagtcaacc gcgtaaggct gctctgagat gtgtgataaa aagcagagag
      661 tgtttctcaa actgtcattc tcaccgtggc tttagcccgg tgttgggtaa tgagaagaac
      721 cggtaaccgt ttcaacggtt tcttgttaga gatcgctgaa accgttaaaa cggttagcag
      781 agaaaaaaac caaccggtaa caccgggcta aagccgcggt gtgaacgaaa ctccacgaga
      841 aggagaagat tatgccactc tacatttcac tggtgcgttg gacgcaacaa ggagtagcac
      901 acgtgaggga cagtcaaaat cggctggact caggacgtaa agcctttcgc gaaattggcg
      961 tcgagattaa gcaggtgcac atgactatgg gtcgctacga tctgatcatg ttgctcgagg
     1021 ctccaactga tgatgcggtg gcgacggcgc tgctctcgct tgcctctcag ggaaatgtgc
     1081 aaaccgaaac gttaaaggcg tggactgagg acgagtatcg gaaaataacc ggaagtatct
     1141 agtctcaagt ctcaagtcca aagtctaagg tttgctcttc aatcttgcgg acaagcttct
     1201 gattggcagc cgcgcgcaag tcctacttga gacttgggac ctggaacttg agactagcga
     1261 gagaatcccg gcccgcgcaa cggtctgcct gcgagtgccg cagttcgctt tccctgatcg
     1321 acgctcaact gaccattgac gattacatag aggacgccct cgggatattg attcggtgcc
     1381 tcgaaggtcg cgcggtcaat tacggtttgc ggattgaaaa tcgcaatgtc cgcaaacatt
     1441 ccttctcgca gtagaccgcg gtccttcaac ccgactcgct tcgcaggcat tcccgtcatc
     1501 ttcgccacgg ccttttcgag cggcagcact ctttgctcgc gtacatagcg tccgagtatc
     1561 cgtggataac taccccaacc acgtggatga gacttggcgc ctgacagggg accatcagtc
     1621 gcgcgcgcgc cgctgtcggt gcagaaactg acgaagggcg acttcatggc agcgagcaag
     1681 tcgttttcac tcatcatgaa gtagatggcg ccagtctgac cgtggtcagc gaggattaga
     1741 tcgaggagcg catcgagcgg atccttattt tgcgcggtag caatttcctc aagccgtttg
     1801 ccttgcagcg actccaactc acggttcacg acagatccaa tcagaatgcc ggcagcgcct
     1861 ccactaccga ggtagatgtt ttcccaatcc ttcgagtcag cactgatttc ttttttcagt
     1921 cgctgtcgca gttcaggatc gcgcaagcga ctcaacattt tttctccgcc accttcctgc
     1981 gcccatggtg gcaaacatgc actcagtgaa gtgctaccgg caatataggg atagacgtcc
     2041 gctgtaatgt cgagtccctg ggcgcgggcc gcactgatct tcttcagcac ctcaggcatt
     2101 cgtccccagt tcttctggta ggcggttttc aaatgccaga tctccaccgg aatgtttgcc
     2161 tgcctggcga tagtgaatac ttcctgcagc gattcatcga gcgcattggc ctcgctccgt
     2221 tggtgagtag cgtagatgcc gccgtgttgc tgcgcgactt ttgcgagctc gacaatctcg
     2281 tccgttttgg cgaaacgcgc aggaacgtat tgaagtgaag ttgagaggcc aagtgcgccg
     2341 tccttcatgg catccgcgac gagctgtttc atctcggcca actcggcggc ggtgggcggg
     2401 cgattatcaa atcccatcac gtgtgcccgc acttgcgttg cgcccacaaa tgtccccagg
     2461 tttactcccg caccttgttt ctcgagtcgc ctgaagtaat cagccagcgt gcgccagtca
     2521 actttcaaat cgaaccgctg aaaaaaatct gcctgctctt tgatcagccg ctcgttgatc
     2581 ggcgcgatcg actcaccttc gccggtgatt tcagtagtga cgcccatcat cactttactc
     2641 atcacccgcg gatcgattag gacgtaggtc tccgattgac ccagcatgtc gatgaaccct
     2701 ggagcaacca ccaaatcgcg ggcctcgatg acacgcttag ccgtggcgct ttttaggttt
     2761 ccaatgcgga cgatgcgatc gcctgttacc gcaagatcgg cgcgataagc ggggcggccc
     2821 gagccgtcca caatctgccc gtcctttata agcaagtcg
//
LOCUS       DASUEX010000366         3340 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1105955, whole genome shotgun sequence.
ACCESSION   DASUEX010000366 DASUEX010000000
VERSION     DASUEX010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3340
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1105955"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="VFV61_06125"
     CDS             complement(<1..473)
                     /locus_tag="VFV61_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900091.1"
                     /translation="MKSTRQVFCLSFILIFSLFADSIRSAHSQAPTPTLLDGFSAEGE
                     ITQRRWEEQFRAVPAASPAREHSRRLTAEPHVAGTKEDYATAVYVRDQMRSYGLAAEL
                     REYDVLLPYPKRPSVVELVAPRRERLGLNEAVIAEDPTSSSSKIIPLFNGYSPSG"
     gene            574..1155
                     /locus_tag="VFV61_06130"
     CDS             574..1155
                     /locus_tag="VFV61_06130"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX4900092.1"
                     /translation="MDSRFYPGVEVDVNKLLDELRNLFDDDYEVQTMQVAATSVLQAR
                     KSSTLRDLTGLSAALTIKITPEGGGTRVEIGMQKWFDKAAVAAVALVLSAGLLLALPA
                     LGAYWQYKITEDAWKTIEGHVAVKAGGYVPPLPGRCGTCGAANSAGSGFCSTCGANLK
                     VGLACPSCGALQRETSARFCNRCGKPLQQSPGS"
     gene            1314..2201
                     /locus_tag="VFV61_06135"
     CDS             1314..2201
                     /locus_tag="VFV61_06135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900093.1"
                     /translation="MKKCLLVLFVSLFACLNVLAQNAPPRVILIVREEIKPGMMPTHS
                     RHAAEYAAMFAKLQTPNERLALVPIAGNENEVVYLNPAASFAEIEKINQDTDKKMSAV
                     NASMQSQVDRLNKEAPALHNNMRDMLAVYRPELSFNPGVNVPQMRYFSVTTVRIRPGH
                     DAQYGEYVQKMLNVAREKAKVTNLHAAVYSVISGTVGGTYIIFRPLKSLAEFDENIGA
                     KVRAAMSDDQKKDADKMYGEAVISSETSTYAFAPRMSFVSKEFASADPDFWHPRTEMA
                     AKPKPKKRAPKAPPTPPAN"
     gene            complement(2286..>3340)
                     /locus_tag="VFV61_06140"
     CDS             complement(2286..>3340)
                     /locus_tag="VFV61_06140"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX4900094.1"
                     /translation="LTQQVSGATVPNASTRFQDFIAGKMISAPDADEVVVTERFARDF
                     GYENLADAAGQTLEFLAPPSASKDENDEEGLPNFFGIPLGEPEPDGKAPGLVAHTFRI
                     AGVLKTELKEGPGQGGLRGLLPRAGIYVPLPAARAWTLEHRTPMGEVALELARQSGAL
                     GDADSEGYDSAVVRVDDPVALTDVRKRLNDAGFSSFSIVDELEQLRTVFLILDSVLGL
                     LGGISLLVASFGIANTMIMSILERTREIGIMKAIGAEDREIKLIFFVEAAVIGLTGGV
                     IGTLVAWGVDGLANRLAYRFILKPQGASYVDFFSLPWWLSLGAILFALAVSILAALYP
                     AARAARIDPVKALRHD"
BASE COUNT          811 a          939 c          901 g          689 t
ORIGIN      
        1 tcgccactgg ggctgtagcc attgaataga ggaataatct tcgagcttga cgaggttgga
       61 tcttctgcaa tcacggcttc gttaaggccg agccgctcgc gccgcggtgc aaccaactcc
      121 acgacagacg gccgcttcgg gtaaggaagc aaaacgtcgt attccctcag ttccgcggcg
      181 agaccataac ttcgcatttg atcgcggaca taaactgcag tcgcataatc ttcctttgtt
      241 cccgcgacgt gaggctcggc tgtcaggcga cgtgagtgtt cgcgagcagg acttgcggcc
      301 ggaacagcgc gaaactgttc ttcccagcgt cgctgggtaa tctcgccctc cgctgaaaag
      361 ccatccagca aagtcggggt tggcgcctgt gaatgagctg agcgaattga atcggcgaac
      421 agcgaaaaaa tcagtatgaa agagaggcaa aatacctggc gtgtgctttt cactgcggaa
      481 tggttctcct tgtcccgatt gtggcgagtg cgcgtcgtaa acttccgctg aacattgttc
      541 cacgtcggcg cgtagaatag ccgaggtcaa atcatggaca gcagattcta tcccggagtc
      601 gaagtagacg ttaacaaact gctcgatgag cttcgtaatc tatttgatga cgattacgaa
      661 gtgcaaacga tgcaagtggc cgcgacgagt gtgttgcagg cacgtaagtc tagcacgcta
      721 cgcgatctca ccggactctc agcggcgctg acaatcaaga tcactccgga aggcggtggc
      781 acgcgcgtgg agattggaat gcagaagtgg tttgacaaag cagccgtcgc cgcggtggca
      841 ctcgtgctgt cagctggctt gctgctcgcg cttccggccc tcggtgctta ctggcaatac
      901 aagatcacgg aagatgcctg gaagacgatc gaaggtcacg tggcggtcaa agcaggcggc
      961 tacgttccgc ctttgccggg acgatgcggc acctgtggcg cggcgaatag cgccggttcc
     1021 ggattctgtt ctacctgtgg cgccaacctg aaagtcggtc ttgcttgccc ctcctgcggt
     1081 gccttgcaaa gagaaacctc ggcgcgattc tgtaatcgtt gcggaaagcc cctacagcaa
     1141 agccctggca gttaggtttc aatgcgattc gtcgaagcat ggctcagagc gatcccgcca
     1201 tcattgactt ccggcaacaa aatttatata aggtcgccgc acgcacggta aatcccagca
     1261 acattacctt cccgaagtta aacgcccagc aaccccccgg aggtgggtta tcgatgaaaa
     1321 agtgtctcct cgtattattt gtttccctgt tcgcctgtct taacgttctt gcgcaaaacg
     1381 caccgccaag agtgatctta atcgtccgcg aagagatcaa gcccggcatg atgccaactc
     1441 acagtcggca cgccgcggaa tacgcggcaa tgtttgccaa actgcaaacg cccaacgaaa
     1501 gactggccct cgtgccgata gcgggcaacg aaaacgaagt tgtttacctg aaccccgccg
     1561 cgtcgttcgc ggaaatcgag aagatcaatc aagacacgga caagaaaatg agcgccgtca
     1621 acgcaagtat gcagtctcag gttgatcgtc tcaacaaaga agcaccggcg ttgcacaaca
     1681 acatgcgcga catgctcgca gtctatcgac cggaactgag tttcaatccg ggtgtaaatg
     1741 ttccgcaaat gcgttacttc tcagtaacga cagttcgcat tcgtccggga cacgacgctc
     1801 agtacggtga atacgtgcag aagatgttga atgtggcgcg cgagaaggcg aaagtcacga
     1861 acctgcatgc agcggtttac tcggtcattt ctggcaccgt tggcggcacc tacattatct
     1921 tccgtccgct caaatcgtta gccgagttcg atgagaatat cggggcgaag gtgagggcgg
     1981 cgatgagcga cgaccagaaa aaagatgctg acaagatgta cggcgaggcc gtcatctcga
     2041 gcgaaacaag cacctacgct tttgcaccac gcatgagttt tgtttccaag gaatttgctt
     2101 cagccgatcc tgatttctgg catccgcgca cggaaatggc agcgaagcca aaaccaaaga
     2161 aacgcgctcc caaagcgcca ccgacaccac cggcgaacta actaaacgag gaatgcagaa
     2221 cgatgaatga tgaacgacta cgttacatca ttcatcgttc atcgttccta attcatcatt
     2281 cttttttagt cgtgcctcag tgctttaacg ggatcgatgc gcgcggcacg cgccgcaggg
     2341 tataacgccg cgagaattga aacggccagc gcaaacagaa tcgcaccgag ggaaagccac
     2401 cacggcaagg aaaagaagtc cacgtaagaa gcgccctgtg gtttgagaat gaaacgataa
     2461 gcgagccggt ttgcgagtcc gtcaactccc cacgctacca gggtcccaat cacgccgccc
     2521 gtcaagccaa tcaccgcagc ctccacgaag aagatcaatt tgatttcacg atcttcggcg
     2581 ccgatggcct tcatgattcc aatctcacgc gtccgttcca ggatcgacat gatcatcgtg
     2641 ttggcgattc cgaatgaagc aacgaggagt gagatgcctc cgagcaaacc taaaactgaa
     2701 tccaggatca ggaatacagt gcgcagttgt tccaactcgt cgacaattga aaagctactg
     2761 aagcccgcat cgttcaaacg cttgcgcaca tccgtgagcg ccaccggatc atccacgcgc
     2821 actactgctg aatcgtagcc ttcactgtcc gcatcaccga gggcgccact ctgacgtgcc
     2881 aactccaggg caacctctcc cattggcgtg cggtgttcca acgtccaggc ccgcgcagcg
     2941 gggagaggca cataaatgcc ggcacgcggc agcaagccac gcaggccacc ctgtcctggt
     3001 ccttccttga gttcggtttt taagacgccc gcgatgcgga aagtgtgagc tacaagcccc
     3061 ggagcttttc catctggctc cggctcgccg aggggtatgc cgaaaaagtt cggcaaccct
     3121 tcttcgtcgt tctcatcctt gctggcgctg ggcggtgcga gaaactcgag agtttggccg
     3181 gcagcatcag caaggttttc ataaccgaaa tcgcgggcga atcgttccgt gactaccact
     3241 tcatcagcat ctggcgcgct tatcatcttg ccggcgataa aatcttgaaa gcgcgtcgac
     3301 gcgttgggca cggtcgcacc actgacctgc tgcgttagga
//
LOCUS       DASUEX010000367         5299 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_636926, whole genome shotgun sequence.
ACCESSION   DASUEX010000367 DASUEX010000000
VERSION     DASUEX010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5299
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_636926"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(204..2471)
                     /locus_tag="VFV61_06145"
     CDS             complement(204..2471)
                     /locus_tag="VFV61_06145"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF012425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HEX4900095.1"
                     /translation="MSTRKSTSKDTNKREVKIPTADKKREELAANSPYRANDAKIDDL
                     QKNTEDSGGEFLTTNQGVRINDNQNSLKAGDRGPTLLEDFILREKITHFDHERIPERV
                     VHARGSAAHGYFQVYESMAEYTKASFLQDPSVKTPVFVRFSTVAGSRGSSDLARDVRG
                     FAVKFYTQEGNYDLVGNNMPVFFIQDAIKFPDLVHAVKPEPHNEIPQAASAHDTFWDF
                     ISNMPESMHMIMWAMSDRAIPRSLRMMEGFGVHTFRFINEAGKARFVKFHWKPVLGVH
                     GVVWDEAQKISGTDPDFHRRDLWEAIENGDFPEWEFGVQIVEEKDEHKFAFDLLDPTK
                     IIPEELVPVRRIGKLTLNRNPDNFFAETEQVAFHTGHVVPGIDFTNDPLLQGRLFSYL
                     DTQLLRLGGPNFHEIPINRPIAPVHNNQRDGFMRQTINKGQSAHDPNSTRGGCPFQAG
                     AEMAGFTSYAEKIDARKIRARSASFFDHFSQATLFYNSQTEPEKNHIIRALRFELGKV
                     ETPAIRERMLGLLAQVDKNLAEQVAEGLGMSVPAKLEKPLNMSIPADGNPAKFQPKRV
                     AQDIEDSPALSMVENPNFPKDTIKTRKIAFLVGDGFDDAAVADMKKALMTAGASAMTV
                     APRLGLLTGADGEQCKADFSFLTASSVLFDAVYIPGGDASVAALKGEPEASNFVQEAY
                     KHCKTIAAAGAGVGLLAVWLGDRFSEANTTGKLIAEEQGVVTSRGPVGANLSAAFIKA
                     IAQHRHWEREQNPTG"
     gene            complement(2621..3277)
                     /locus_tag="VFV61_06150"
     CDS             complement(2621..3277)
                     /locus_tag="VFV61_06150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900096.1"
                     /translation="MKENTVLPNHVTELLYQALETEKGGVQIYTTALRCAVNPDLKQE
                     WQKYLEQTKNHVQVLNDTLNRLGLELEIETPGRKIVRHIGESLVKAMEMALRAGDPKS
                     AQIVAAECVTLAETKDHLNWSLIGELAKQVGIETSEALTEAYGQVEDEEDEHLYHTEG
                     WTRELWIEALGMPAVLPPPEEEQDVETAREAAQAKESRKTKANSSKNQKVKKAAGSGR
                     "
     gene            3790..4446
                     /gene="mtnB"
                     /locus_tag="VFV61_06155"
     CDS             3790..4446
                     /gene="mtnB"
                     /locus_tag="VFV61_06155"
                     /EC_number="4.2.1.109"
                     /inference="COORDINATES: protein motif:HMM:TIGR03328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylthioribulose 1-phosphate dehydratase"
                     /protein_id="HEX4900097.1"
                     /translation="MMQAPGHMPNSEFTQLASGLAATARALHARGWLLGTSGNLSAMV
                     QREPLLLAMSPSGVDKGELSAEQILLVDEHARIVNGSGKPSDETLLHIEIVKQRQAGA
                     VLHTHSVWNTIISDLFADQSAVAIEGYEMLKGLAGVHTHKHREWLPIIENSQDMRELA
                     AKISIVLTEQPNAHGFLLDKHGLYSWGDNLREAKRHIEILEFLIEARVRTLELKRRGN
                     "
     gene            4449..5024
                     /locus_tag="VFV61_06160"
     CDS             4449..5024
                     /locus_tag="VFV61_06160"
                     /inference="COORDINATES: protein motif:HMM:NF015065.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX4900098.1"
                     /translation="MALLTICDQNRTLNDEREIRDYLLTLGIDYERWPTTERVAPEAS
                     AEKVLEAYAEEIEKLKASGGYVTADVIDVTTATPGIEEMLAKFNREHWHDEDEVRFII
                     SGNGLFHIHSKSGEIVAIEVSAGDLIRVPRGTLHWFNLCADRRIRAIRLFQDKSGWTP
                     HYTDSLVDRNYMPVCLGRPEGEEISVQRAQR"
     gene            5021..>5299
                     /locus_tag="VFV61_06165"
     CDS             5021..>5299
                     /locus_tag="VFV61_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900099.1"
                     /translation="MTDAATKTRALLLDIEGTVTPISFVHDILFPFARTHVRNYLIQH
                     ATTAEVEKDTQALFREYSIDQQKNEQLPQIDTGVWSIDSIIAYVNWLIE"
BASE COUNT         1184 a         1420 c         1380 g         1315 t
ORIGIN      
        1 ccccgatttg atttgcctca cctgcgccgc ctgaagaaga aaaacaagat gactatgacg
       61 agcagtaagc tcacgagact gcccggccag agccaccacc caccgtcgct actgtagccg
      121 ccaaccatca cgcaagacaa ttgcgaaaac gcgagcatca atgagagcgc agatattctt
      181 ttcataagat cccttgccct cactcaaccc gttggattct gttcgcgctc ccagtggcgg
      241 tgctgagcga tggccttaat aaaggcagca gacaggttgg ccccgacagg cccacggctc
      301 gttaccacac cctgctcctc tgcaattagt ttcccggttg tgtttgcttc ggaaaatcta
      361 tcacccagcc acacagccaa taagccgacg cccgctcccg cagccgcaat cgttttgcag
      421 tgtttgtacg cctcctggac gaagttagaa gcttccggtt cccctttgag agcagccacg
      481 ctcgcgtccc ctcctggaat gtagacggca tcgaacaaga ctgacgaagc ggtgaggaaa
      541 ctgaaatcag ctttacattg ttctccgtca gctcccgtca acagaccgag tcggggtgcg
      601 acggtcatgg ctgacgcgcc tgcggtcatg agtgctttct tcatatcagc cacggctgca
      661 tcgtcgaatc cgtcgccaac caggaaagcg atcttgcggg tcttgatcgt gtctttagga
      721 aagttgggat tctcaaccat gcttaaggca ggcgagtctt ctatgtcctg ggcaactcgc
      781 ttcggctgaa actttgcagg attaccgtct gccgggatgc tcatgtttag cggcttttcc
      841 agttttgccg gcacgctcat ccccaaacct tcagcgacct gttcagcgag attcttatct
      901 acttgtgcaa gcagcccgag cattcgttcg cggatggctg gagtctcgac cttacccagc
      961 tcgaagcgga gtgcgcgaat gatgtggttc ttctccggct cggtttggct gttatagaag
     1021 agagtcgcct ggctgaagtg atcaaagaag ctggcgctgc gggcgcgaat cttgcgcgcg
     1081 tcgatttttt ctgcgtagct ggtaaatccc gccatctctg cgcccgcctg aaacggacag
     1141 ccaccgcgag tagagttcgg atcgtgagcc gattgacctt tattgatggt ctggcgcatg
     1201 aatccgtcgc gctgattatt gtgcacgggc gcgatgggcc gattgatcgg gatctcgtga
     1261 aaattcggac cacctaaacg aagcagttga gtatccaggt atgaaaaaag tcgcccttgc
     1321 agcaacggat cgttggtgaa gtcgatcccc ggaactacgt gaccagtgtg gaaagcaacc
     1381 tgctcagtct cagcaaagaa gttgtcggga ttgcgattga gcgtcagctt gccgatgcgg
     1441 cgcaccggaa caagttcttc gggaataatc ttcgtcggat cgagcaaatc aaaggcaaat
     1501 ttgtgttcgt ccttctcctc gactatctgc acgccaaact cccactcggg aaagtcgcca
     1561 ttctcgatcg cttcccagag atcgcgccga tggaagtccg gatctgtacc ggagattttc
     1621 tgggcttcgt cccacacaac cccgtgcacc cctaggactg gtttccagtg aaacttcaca
     1681 aagcgcgcct tgcctgcttc gttaataaaa cggaaagtgt gaacgccaaa accttccatc
     1741 atgcgcaagc ttctggggat cgcgcggtcg gacatcgccc acatgatcat gtgcattgac
     1801 tcaggcatgt tggagatgaa atcccagaag gtgtcgtggg ctgatgccgc ttgtggaatc
     1861 tcattgtgcg gttccggctt caccgcatga acgagatcgg gaaacttgat tgcgtcctga
     1921 ataaagaaca ccggcatgtt gttgccgacg aggtcgtagt tcccttcctg cgtataaaac
     1981 ttcactgcga aaccccggac atcacgggcg aggtcggacg aacctcgtga acctgccact
     2041 gtcgagaaac ggacaaacac cggagttttg acggaagggt cctggagaaa tgaagccttc
     2101 gtatactcgg ccattgattc gtacacctga aaatagccat gcgctgccga accgcgcgcg
     2161 tgcactaccc gttcaggaat tcgttcgtga tcgaaatgag tgatcttctc gcggaggata
     2221 aaatcttcca gcagcgtcgg gccgcgatcg ccggctttga gtgagttctg attgtcgttg
     2281 atgcgcacac cctggttggt ggtaagaaac tctccgccac tgtcctcagt gttcttttgc
     2341 agatcgtcga tcttggcgtc gttggcacga tatggagaat tcgccgctaa ctcttctcgc
     2401 ttcttatctg ctgttggtat tttcacttcc cgcttgttgg tatccttgct cgtagatttc
     2461 ctggtgctca tattttttcc ttttaattct tattgaacgg tgtgcgcgga ctgcgttgtc
     2521 ctgactctgt tgaggcggca atccgtggct gcctcttgtt cggaattcag gggcgcccac
     2581 agagggacgc ccatgcacga gatccagcat tgacgcgatt ctatctgccc gatcctgcgg
     2641 ccttcttgac tttctgattc ttcgagctgt ttgcttttgt cttgcggctc tctttagctt
     2701 gtgcggcttc ccttgccgtc tcgacgtctt gctcttcctc cggcggcggg aggacagccg
     2761 gcattcccaa cgcttcgatc caaagctcac gcgtccaacc ttcggtgtgg tagaggtgct
     2821 cgtcttcttc gtcttccact tgaccatagg cttccgtcaa cgcttcactc gtttcaatgc
     2881 cgacctgttt ggccaattca ccaatcaagc tccagttgag atgatccttg gtttccgcaa
     2941 gcgtgacgca ctccgccgcg acgatctgcg ctgacttggg atcgccagcc cgcaacgcca
     3001 tctccatggc tttcaccaga gactctccga tgtgccgcac aatttttcga cctggagtct
     3061 cgatttccaa ctccaggccc aagcggttca aagtgtcgtt gagaacttgg acgtgattct
     3121 tcgtctgctc cagatacttc tgccactctt gcttcagatc cggattcacg gcgcaacgta
     3181 gcgccgtggt gtagatctgc acgccgccct tttctgtttc cagcgcctgg tacagcagtt
     3241 ctgtaacgtg gtttgggagt acagtatttt ccttcatgtc ctgggatttc ctttcgtaat
     3301 ggtgaattcg tcttcctgaa ttgattgcaa gcaacagcgc ataccgggcc tgcaattatc
     3361 aggtcggcga gcgctatcgg ttttcgcgaa taagaacgga ctgtttatga gcaagaagga
     3421 tgccgtttca gatcacaaat tctctcggcc attgaaggag tgagttggtt tgactacttg
     3481 aagctttcca aggaatgtga cccaagtttt gaatagacac gagaagcaca tcggcgtttg
     3541 tcttctgtac taattgtagg cagcgagatt caacctaacc ttaaaagaga acagagaaga
     3601 ttgccgattg ccgatctcta aattgggtag gcaaattagt cttttctagc atagcgaggt
     3661 tactccgagt agaatgagcc gcttttccta tctaacgatt aaaatcgcac cttccgcatg
     3721 cccaggagga ggcgactggt tacttcccgt tcttgaattt gaacacttag aggcgggcag
     3781 caagcattga tgatgcaggc accaggacac atgccgaatt cagagttcac ccaacttgcg
     3841 tcaggcctgg ctgcaacagc cagggctctg cacgcgcgcg gctggttgct tggcaccagc
     3901 ggaaacctca gtgcaatggt tcagcgagag cccttgctgc tggctatgag tccgagcgga
     3961 gtcgacaagg gtgaactctc cgctgaacag atcctgttgg tcgatgaaca tgctcgtatc
     4021 gtcaatggaa gtggtaagcc gtcagacgaa acattgcttc atatcgagat cgtaaagcag
     4081 cgacaagccg gcgccgtgct gcacacccat tcggtttgga ataccattat ctcggacctg
     4141 tttgctgatc aaagcgccgt tgcgattgaa ggctatgaga tgttgaaagg actggcaggc
     4201 gttcatacgc acaagcatcg tgaatggctt ccgattattg aaaactctca ggacatgcgt
     4261 gaacttgccg cgaagatcag tatcgtgtta accgagcaac cgaacgcgca tggctttctg
     4321 ctagataaac acggcctcta ttcatgggga gacaacctgc gcgaggccaa acgacatatt
     4381 gagattctgg aattcttgat cgaggctcgg gttcgcaccc tggaacttaa acgacgaggc
     4441 aactgatcat ggccctactt acgatttgcg atcagaatcg aaccctgaac gacgagcgag
     4501 agattcgcga ctacttactt accctgggca tcgattatga gcgttggccg actactgagc
     4561 gggttgcgcc agaggcctct gccgaaaagg ttctggaagc ctatgccgaa gaaattgaga
     4621 agctgaaagc cagcggcggt tatgtcacgg ccgacgttat tgacgtaacg actgctactc
     4681 ccgggattga agaaatgctg gcgaagttca atcgcgaaca ctggcatgac gaagacgagg
     4741 ttaggttcat catttccggt aatggtcttt ttcatattca tagtaagagt ggcgaaatcg
     4801 tagctatcga agtcagcgct ggtgatctaa ttcgggttcc gcgtggcaca ctgcactggt
     4861 tcaacctgtg tgctgatcgc cgcattcgag ctatccgttt gtttcaggac aaatccggct
     4921 ggacgccgca ttacactgac agtctggtcg atcgaaatta catgcctgtt tgtcttggca
     4981 ggcccgaagg cgaggaaatc agcgtccaaa gagcgcagcg atgaccgacg cggcgacgaa
     5041 gacacgtgct ctgctgctcg atatagaagg cactgttact cccatctcat tcgttcacga
     5101 catcctcttc ccttttgctc gaactcacgt aagaaactat ttgattcagc atgcaaccac
     5161 cgccgaggtg gagaaagata cccaagcact ttttcgggag tactcgattg accagcagaa
     5221 aaatgaacag ctgccgcaaa tcgacacagg agtctggtcg attgattcga taatcgctta
     5281 cgttaactgg ctgattgag
//
LOCUS       DASUEX010000368         3434 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1106071, whole genome shotgun sequence.
ACCESSION   DASUEX010000368 DASUEX010000000
VERSION     DASUEX010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3434
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1106071"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1307
                     /gene="asnB"
                     /locus_tag="VFV61_06170"
     CDS             <1..1307
                     /gene="asnB"
                     /locus_tag="VFV61_06170"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEX4900100.1"
                     /translation="GKITIKKYWDVSFAKASRYKRSDQDYIAEWGELFRKSVQLRLMA
                     DVPLGMFLSGGIDSSAIAAVMSTLVDEPIKTFSVAFAEREANELEYARLVARAFKTDH
                     HEVVISPDQFFEALPELIWHEDEPLAHPSSVALYFVSELASQHVKVVLTGEGSDELMA
                     GYGRYHKTILNLALGERYQRMVPSGVRDLVRDRIAGMRGSKLQRTFLNRSTDLESIYF
                     DNFAVFPRSDQEQLLTRETIDSIGGIDPYCDVRRVLADTDAKSILDRLLYADIKTYLH
                     ELLMKQDQMSMAASVESRVPFLDHKLVEFTSSLPEQLKLHGSTTKYVLRQSMKGVLPD
                     QILTRPKMGFPVPIGAWFRGAYAKVLDEYILSNRAMDRSIFNQEYTRKLVSQHQRGEA
                     NHSERLWSLVNLEMWLRRFIDGEDISGGWKAAGETVLASARA"
     gene            1339..2091
                     /locus_tag="VFV61_06175"
     CDS             1339..2091
                     /locus_tag="VFV61_06175"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX4900101.1"
                     /translation="MGFIGGSLGYNLLRLISSNGETGYCDGSAYQGVSKIEKLLGNRI
                     WEETKGKVVIDFGCGDGDDAVDVAERGAKRVIGIDIRERALAKARASAVARGVADRCT
                     FATKTDVQADVILSLDAFEHFDDPAAILRIMRRLVKDDGCVISAFGPTWYHPLGGHLF
                     SPFPWAHLLFTENSLIRWRSDFKTDGATRFCEAEGGLNQMTIGRFEALIAKSDFKFEE
                     FEAVPIRKLGRFANPLTREFTTAIVRCKLVPR"
     gene            2070..3419
                     /locus_tag="VFV61_06180"
     CDS             2070..3419
                     /locus_tag="VFV61_06180"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX4900102.1"
                     /translation="MQARPSLKSYMRILWLKTELLHPLDKGGKIRTYNMLKQLKQDHH
                     VTYLTLDDGSASADDRSRALEYCHDLVCIKHSRREKFTPGFYFELLLNLVSSQPYAIR
                     KYQAPAMWREIVERERSKSFDVLICDFLAPAANVPETLATPALLFQHNVEAMIWKRHY
                     ETQTDPVKKAYLYGQWRKMLKFERQMCRRFDCVIAVSADDREQMKREYGAETVFDVPT
                     GVDTDFFRPSGEAQASAHNIVFTGSMDWLPNDEAIRYFRREIMPLIKKKVPDATLTVV
                     GRNPTPALMELAKTDPSLIVTGRVDDVRPYIENSAAYIVPLRIGGGTRLKIFEAMAME
                     KAVVSTTIGAEGLPLTDGVELLLADEPAAFADAAVHLLTDKPYATELGQRAAAIVRKN
                     YSWRQVTEKFISICLESAKSASPAVADGSEINREGSLKSTIPIADPPVTASGTELIH"
BASE COUNT          832 a          844 c          956 g          802 t
ORIGIN      
        1 atggcaaaat cacaattaag aagtattggg acgtcagttt tgcaaaggct tcacggtaca
       61 agcgtagcga tcaagactac attgccgagt ggggcgaact attcaggaag tcagtacagc
      121 tccgattaat ggcggacgtt ccgctcggaa tgttcttgtc cggaggaatc gattcgtcgg
      181 caatagccgc ggtgatgagt acgttggtcg atgaacctat caagacattt tctgttgcgt
      241 ttgccgaacg tgaagctaac gaactcgagt atgcgcgact cgtggcgcgc gctttcaaga
      301 cggaccatca cgaggtagtt attagcccgg accagttttt tgaggccttg ccggagttaa
      361 tctggcacga agacgagccg ctcgctcatc cttccagcgt cgcgctttac ttcgtttcgg
      421 agcttgcttc ccagcacgtc aaagttgtct taaccggcga aggcagcgac gaactaatgg
      481 cgggatatgg tcgctaccat aaaacgatac tgaacctggc gctgggtgaa cgctaccagc
      541 gtatggttcc ctccggtgtc agagatttgg tgcgcgatag gatcgccggc atgcggggat
      601 caaaactgca gcgaactttt ctaaaccggt cgaccgacct cgaaagcatc tactttgata
      661 acttcgcagt gtttcctcgc tcggaccagg aacaattgtt gacgcgtgag accatcgata
      721 gcattggtgg tatcgatccg tactgcgatg ttcgacgcgt actggcagac actgatgcca
      781 aatcgatttt agatcgcttg ctctatgcgg acatcaaaac ttatttgcac gagttactga
      841 tgaagcagga tcagatgagc atggcggcgt cggtcgagag ccgcgtgccg ttcctggatc
      901 acaagctggt ggagtttaca tcgagtcttc cggagcaatt gaaactgcac ggttcgacga
      961 caaagtatgt gctgcggcaa agcatgaaag gtgttttgcc tgaccagatc ctgaccagac
     1021 ctaagatggg atttccggtg ccaatcggcg catggtttcg tggcgcttac gcgaaagtgt
     1081 tggatgaata tattcttagt aaccgcgcca tggatcgtag catcttcaat caggaataca
     1141 cccgcaagct tgtaagccag catcagcgtg gcgaggcaaa tcactctgaa cggctgtggt
     1201 ccctcgtcaa tttggaaatg tggttaagac ggtttatcga cggagaagat atatccggcg
     1261 gttggaaagc tgccggtgaa accgttctcg cgtcggcgcg cgcctagatt tgcgatcggt
     1321 attatcggag gacttcggat gggttttatt ggaggctcgc ttgggtacaa cttgcttcgc
     1381 ctgatcagct ccaacggaga aaccgggtat tgcgatggca gcgcctatca gggcgtcagc
     1441 aaaattgaaa aactgcttgg taaccggatt tgggaagaaa cgaaagggaa ggtcgtaatc
     1501 gatttcgggt gtggcgatgg cgatgacgct gtcgatgtgg cagagcgggg agcgaaaaga
     1561 gttatcggaa tcgatattcg cgaacgtgcg ttagcaaagg cgagggcgag cgctgtcgcg
     1621 cggggtgtag ccgaccgttg taccttcgct actaaaacag atgtgcaggc tgacgtcata
     1681 ttgtcacttg acgccttcga gcacttcgat gaccccgcag ctatcctgcg aatcatgcgt
     1741 aggttggtga aagacgacgg ctgcgtgatc tctgcatttg gtcccacgtg gtatcaccct
     1801 ctcggaggtc atcttttttc tccctttccc tgggcacatc tgcttttcac tgagaactca
     1861 ttgattcgct ggcggtcgga cttcaaaacg gatggcgcga cacgcttttg cgaagctgaa
     1921 ggtggcctca accagatgac cataggacgc ttcgaggcct taatcgccaa aagtgacttt
     1981 aagtttgaag aattcgaggc tgttccgatc aggaaactcg ggcgctttgc taacccgctg
     2041 acgagagagt ttacgacagc gatcgtgcga tgcaagctcg tccctcgctg aaatcctata
     2101 tgcgcatttt gtggctaaaa actgaattgc tgcacccgct cgataagggc gggaagatcc
     2161 gcacttacaa catgctaaag cagctaaaac aggatcatca tgttacttac ctcacgcttg
     2221 acgatgggtc tgccagtgct gacgaccgca gtagagcgtt agagtattgc cacgacctgg
     2281 tctgcattaa gcatagtcgc cgcgagaagt ttacgcccgg tttctacttc gaattgttgt
     2341 tgaatttggt gtcgtcccag ccgtacgcaa tcagaaagta ccaagcgcca gcaatgtggc
     2401 gcgagattgt cgagagagaa cgtagcaagt ctttcgacgt gctcatctgt gatttccttg
     2461 cgcccgccgc gaatgttccg gaaaccctgg caacaccggc cttgctcttt cagcataacg
     2521 tcgaggcgat gatttggaag cgccactacg aaacgcaaac cgatccagtc aagaaagcat
     2581 atctctatgg ccagtggcgg aagatgctca agttcgaacg gcagatgtgc cgccgctttg
     2641 attgcgtgat agcagtttcc gcagatgacc gtgagcaaat gaaacgggaa tatggggcgg
     2701 agactgtctt cgacgttccc accggcgtcg ataccgactt ttttcgaccg agcggcgagg
     2761 ctcaagcttc agctcacaac atagtgttca ctgggtcgat ggattggctt cccaatgacg
     2821 aggcaattcg ctacttcagg cgagagatca tgcctctgat caagaagaaa gtgcccgatg
     2881 caacgctcac ggtggtcggc agaaatccca cgccggcatt gatggagtta gctaaaacgg
     2941 atccttctct catagtcacg ggccgtgttg acgatgtacg accttacatc gaaaactccg
     3001 cggcttacat cgttcccttg cggatcgggg gtggaacgcg actcaagatc ttcgaggcaa
     3061 tggcaatgga aaaagccgtc gtctcaacga caatcggggc cgaaggtcta ccactaacgg
     3121 atggggttga gttgttgctg gctgacgaac cggcagcgtt tgccgacgca gctgtccacc
     3181 tactaactga taagccatac gccacggagc tgggccaaag agcagctgcg attgtcagga
     3241 agaattacag ttggcgacaa gttactgaga agttcatttc gatctgttta gagagcgcga
     3301 aatcagcatc acctgcagtc gcagatggat cagaaatcaa tcgcgaggga tcacttaagt
     3361 ctacaattcc aattgcggac ccgcccgtaa cagcaagtgg aacagaactc atccactaac
     3421 gatcttcgtt aggc
//
LOCUS       DASUEX010000369         3145 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1312868, whole genome shotgun sequence.
ACCESSION   DASUEX010000369 DASUEX010000000
VERSION     DASUEX010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3145
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1312868"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1723)
                     /locus_tag="VFV61_06185"
     CDS             complement(<1..1723)
                     /locus_tag="VFV61_06185"
                     /inference="COORDINATES: protein motif:HMM:NF018899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HEX4900103.1"
                     /translation="MGQSWRQQLGINSTASGKLGWRILASLFLMTLVSLPAAAVTNGD
                     ERGFDAYEGRRVTAIEIVFEGSQPDASAEASFLAVLKVLPNTEFSAVRVRDSLQALFE
                     TQRVANARVEVIETAQRSGPIRLRFVVQRQVQISEVSFEIGPSSGIPVAADELRARLS
                     LVQPGSRLSKQIITRNADEIQVYLRDRGYFNAVVEPLEQLDSSGTRAVVTYRITLNEQ
                     ARVESFNIGITGFDPTPLRTQLRLQPGAFFTREALAEDVKRIRDAIIAKGNLAPQLED
                     PRVERDHETNRMTINLQGGIGPKVTVVIQDFELNEKTQRELLPVKREGNIDLSAIEEG
                     ARRLRNKLQEEGYFFAEVTPVCSVVPPVIGTPASGTGETCENLDPEALSGRTVEIQYQ
                     IEKGRRFRLTDIRITGTNKLTFEDVEADLKSQKASALGLIPFLGYGRGYTSLTLLEQD
                     RRTVRNYMRELGYRRADVEVLQGVSLNGENLIITFNVVPNELTRVAGVEVRGNKIYTE
                     KQLRDELKTVIGAPFSRAQARFDGDRVLALYSREGYANAQVELSVVELPRKGDEDQVR
                     LIYTITCR"
     gene            complement(1727..>3145)
                     /locus_tag="VFV61_06190"
     CDS             complement(1727..>3145)
                     /locus_tag="VFV61_06190"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HEX4900104.1"
                     /translation="QFSPSEVFFEKTQFTGPGTNILLGGRLAVGPGGRQTFTADGQLN
                     LRVLNGLSPDVFTSGVADVAVRITGSYEQPRLNGTASVASGSVSVLLGNERWTITNLK
                     SVVRFTSDQAQIESLAGSMGGGHVSASGGARLEGFSLAEFLINLHGENVTVPFPTDFR
                     STVDADLEIKGSAREQLVGGTVILRRSEYTKDIELTDLINFRREESIEEGGELEFTRA
                     AVFNDLKVEGRNALVVRNNLADLIGSVSLQVNGPVKDPVISGRITATSGTLNFRNDRY
                     EITRALMDLPPRRDADPIVNIQGEAQIRGYRVNVTLTGPLSQPQAVVGSEPSLPQADV
                     VSLITTGQLSAGDTSASVLSQSGLGTATSLLTDALINAPAQRATSKLFGLSRFEINPV
                     IGGRSGSTPGARLTVGKRINKNLSITYSTNVASDPNQILAVEYRVSDRISFIAQYEQA
                     STRRLSTQNDNFSFEIRFRKRF"
BASE COUNT          686 a          884 c          867 g          708 t
ORIGIN      
        1 agcgacaagt aatcgtgtag atcaaacgta cctggtcttc gtcgcctttg cgcggcagtt
       61 ccaccaccga gagctcaacc tgggcgttgg cataaccctc tcgcgaatac aaagcgagca
      121 cgcggtcgcc atcgaagcgc gcttgcgccc gcgagaaggg tgcgccaatc acagtcttca
      181 gctcgtcacg cagttgtttc tcggtgtaaa tcttattgcc ccggacttct actccggcaa
      241 cgcgagtcag ttcattgggt acgacgttga aggtgatgat tagattttcg ccgtttagcg
      301 agacaccttg cagcacctca acatcggcgc gccgatagcc cagctcgcgc atgtaattgc
      361 gcacagtgcg ccggtcctgc tccaacaagg tgagactggt gtagccgcgg ccgtaaccga
      421 gaaacggaat caacccgagt gcgctggcct tctgactctt cagatcggct tccacatctt
      481 cgaacgtcag tttgttggtt ccggtgatac gaatatcagt aagacggaag cgacggccct
      541 tttcaatctg atattgaatc tccaccgtgc ggccgctgag ggcttcggga tccagatttt
      601 cacaagtttc gccggtgcca ctggcgggcg ttccgattac cggcggcacc acgctacaaa
      661 caggcgtcac ttccgcgaag aaataccctt cctcctggag tttattgcgc aggcggcggg
      721 cgccctcttc aatcgccgag agatcgatgt tgccttcccg cttgaccggc aaaagttcgc
      781 gttgggtttt ttcgttaagt tcgaagtcct gaattacgac ggttaccttc ggaccgatcc
      841 cgccttgcaa attaatagtc atgcggttgg tttcgtggtc gcgttcaacg cgcggatctt
      901 cgagctgcgg cgcgaggttt cccttagcta tgattgcgtc acgaatgcgt ttgacgtctt
      961 cagcaagcgc ttcgcgggta aagaaagcgc ctggttgcag gcgcagctgc gtacgcaatg
     1021 gggtcggatc aaatccggta ataccgatgt tgaatgactc gacgcgagcc tgttcgttca
     1081 aggtgatgcg gtaagtcacc accgcccggg tgccgctgct gtcgagttgc tccagaggtt
     1141 cgacgaccgc attgaagtaa ccgcggtcac ggaggtagac ctgaatttcg tcggcgttgc
     1201 gagtgatgat ctgttttgaa agtcgcgatc caggttgtac cagactcagt cgggcccgta
     1261 attcatccgc cgctaccggt attccagagg acggtccgat ttcaaaacta acctcgctga
     1321 tctgcacctg gcgctgaact acgaaacgca ggcgaatcgg tccgcttcgc tgcgcagtct
     1381 cgatcacttc gacccgcgca ttggcaacgc gttgcgtttc aaataaggct tgcaacgagt
     1441 ctcggactct taccgcagaa aactccgtgt tcggcagaac tttgagcacc gccagaaagc
     1501 tcgcttcggc gctggcatcc ggctgcgagc cttcaaagac gatctcaatc gcggtcaccc
     1561 gccggccttc ataagcatca aacccgcgtt cgtcgccatt ggtcacagcc gcggccggga
     1621 gcgaaaccag cgtcataagg aaaagcgacg cgaggattct ccagcccagt tttcccgacg
     1681 ctgtggaatt gattcccaat tgttgccgcc agctctgccc catgctttag aaccgtttcc
     1741 tgaaccggat ttcaaaactg aagttatcgt tttgcgtgct aagtcgccgc gtggaggctt
     1801 gttcgtactg cgctatgaag gagatacggt ccgacacgcg atattcaacg gccaggatct
     1861 ggttcgggtc agaagcgacg ttggtcgaat aagtaatcga aagattcttg ttgatgcgtt
     1921 tgcccacagt caggcgcgct cccggagttg atccgctgcg cccgccgatc accggattaa
     1981 tctcaaaacg gctcaaaccg aagagcttac tggtggctcg ctgggcagga gcattaatca
     2041 gtgcgtctgt aagcaagcta gtcgctgtgc caagacctga ctgcgaaagt actgaggcgc
     2101 tcgtgtcacc agcggagagc tgacctgtgg taatcagcga gacaacatct gcctgcggta
     2161 aggacggttc tgagccgaca actgcttgcg gctgtgaaag tggtccggtc agggtcacat
     2221 tgacgcggta gccacgaatc tgagcttcgc cctgaatgtt gacaatggga tcggcatcac
     2281 gacgaggagg caagtccatc aacgcgcgcg tgatctcgta acgatcattg cggaagttga
     2341 gcgtaccgct ggtggccgtg attcgtccgg agataactgg atcctttacc ggaccattca
     2401 cttgtagtga gactgatccg atcaggtcag caagattatt tcgaaccacc aatgcgttgc
     2461 ggccttctac cttcaagtcg ttgaagacag ccgcgcgcgt gaattccaat tcaccgcctt
     2521 cttcaatcga ctcttcgcga cggaaattga tcagatcggt caactcaatg tctttcgtgt
     2581 attccgagcg acgcaagatg actgtgccac cgacaagttg ctctcgcgct gagcccttga
     2641 tttcgagatc cgcgtcaacg gtggaacgga agtctgtggg aaacgggacc gtcacgtttt
     2701 ccccgtgcag gtttatgaga aactccgcaa gcgaaaatcc ttcgaggcgg gcgccgcctg
     2761 aagcgctgac atgaccaccg cccatcgagc cggccagtga ctcaatttgc gcctggtcag
     2821 atgtgaaccg aacgactgac ttgagatttg ttatcgtcca gcgttcatta cccagcaaca
     2881 cggatactga accgctggcg accgacgccg tgccgtttaa gcgcggctgt tcatagctcc
     2941 cagtgatgcg caccgccaca tcagcaacac cggaagtgaa gacatcggga gacaaaccgt
     3001 tcagaacacg caggttcagt tgaccatcgg cagtaaaggt ttgcctgccg ccgggaccaa
     3061 cggccagccg gccgcctaac agaatatttg tgcccggccc ggtgaattga gttttttcga
     3121 agaaaacttc gctcggtgaa aattg
//
LOCUS       DASUEX010000370         2371 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_871803, whole genome shotgun sequence.
ACCESSION   DASUEX010000370 DASUEX010000000
VERSION     DASUEX010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_871803"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1095
                     /locus_tag="VFV61_06195"
     CDS             <1..1095
                     /locus_tag="VFV61_06195"
                     /inference="COORDINATES: protein motif:HMM:NF015140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4214 domain-containing protein"
                     /protein_id="HEX4900105.1"
                     /translation="PSPTPTPTGTPAIALSAANYSVPENGNQVTVTLTRLGDPAGIAS
                     VKYASSDSAGLNNCNVVNGIASSRCDYAAVVGLVTFASGESLKTISIPLVDDAYAEGN
                     ESFTLSLSNATGAGLGNVSTATITINDNEVVNGSNPIEVTAFFVREHYIDFLGREPDP
                     AGYTGWQNVINNCASGDTSCDRIHVSGAFYQSPEFQERGYFVYRFYSVSFGRKPDYAE
                     FLPDFARVSGFLSNQQLEGARVAFVADFIARPAFATKYDGLSNAQFVETLVATAGVTL
                     TVKQDLIDALNNGTKTRAQVLREIVEHAVVTQKFFNQAFVVMQYFGYLRRDPDAAYLN
                     WIQELNSSGNARTMINGFVNSLEYRARFGN"
     gene            1250..1861
                     /locus_tag="VFV61_06200"
     CDS             1250..1861
                     /locus_tag="VFV61_06200"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX4900106.1"
                     /translation="MKKNLISTLLVTSFLFASALVAVSGRTIEGEVPAPPAIGSTIAD
                     FSLPDGNGKEHTLASLAGKNGSVLIFMAVQCPVSNAYNERMEKLAQDYQAKGINVIGI
                     NSNVAESADAVKAHAAEKHLTFTILKDAGNKVADRLGASVTPEAYFLDAANKLLYRGR
                     IDNSKNADGVTASELRDAIDATLAGKPITKTTANAFGCTIKRA"
     gene            1866..>2371
                     /locus_tag="VFV61_06205"
     CDS             1866..>2371
                     /locus_tag="VFV61_06205"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEX4900107.1"
                     /translation="MKLTRKLLWLGLVLSFMLSASPADAQRKQTSKKSPAKISVAAID
                     ADGLVALLKRNATKNKPLLVNFWATWCDPCREEFPDLVKIDKQYRPQGLDFTAVSIDD
                     LSEIKTTVPQFLREMQVTVPTYLLNVSDPEVAMSRVDPQWSGALPATFLYDAQGNLVY
                     KRLGRIKV"
BASE COUNT          555 a          634 c          638 g          544 t
ORIGIN      
        1 ccctctccaa cgccgacacc cacaggcaca cctgcgatcg cgctgagtgc ggcgaactac
       61 agcgttcccg aaaatggcaa tcaggtgacg gtgactttga ctcgcctcgg agatccagcg
      121 ggcatcgcct cggtgaaata cgccagcagc gactcagccg gtctgaacaa ctgcaacgta
      181 gtgaatggca ttgcctcatc gcggtgcgac tatgctgccg tggttggctt ggtgaccttc
      241 gcatccggtg aaagcctgaa gacgatctcg attccgctag ttgacgatgc ttacgcagaa
      301 ggaaatgaga gtttcacgct tagcttgagt aatgccaccg gcgccgggct gggaaacgtg
      361 tcaacggcaa caatcaccat caacgacaat gaagtagtga acggtagtaa tccgattgag
      421 gtaacggcct tcttcgtacg cgagcactac attgactttc tgggacgcga accagatccg
      481 gccggctaca ctggttggca gaacgtaata aataattgtg cgtcgggcga tacgagttgt
      541 gaccgaattc atgtttcggg agctttttac cagtctccag agttccagga gcgtggttat
      601 tttgtttatc ggttctattc ggttagcttc ggccgcaagc cggactatgc tgagttcctc
      661 cctgactttg ctcgcgtcag cggttttctt tccaatcaac aactcgaggg tgcccgagtg
      721 gcttttgtcg cagatttcat cgcgcgacca gcctttgcga ccaagtatga cggcctgagc
      781 aacgcgcaat ttgtggagac gctcgttgcc accgcaggcg tgacacttac agttaagcag
      841 gatctgatcg acgcattgaa caatggaacg aagacgcgag cccaggtctt acgcgagatt
      901 gttgagcacg cagttgtaac gcagaagttc ttcaaccagg catttgtggt gatgcagtat
      961 ttcggttatc tgcgccgtga cccggacgcc gcctacttga attggatcca ggaactgaac
     1021 agtagcggta atgcgcgcac catgatcaat ggtttcgtga attcactcga gtatcgcgcc
     1081 cgcttcggta attgatgatt agtgttaaca cctctcacag tcagttaggc cgggctcggc
     1141 tgcagctgct ccgtcccaat cattcaagct aacttgcctc ctcctgagcc cgctgttaat
     1201 ctttgcctac attccccaag cgttctaatc cagaaggagt ttcccagtca tgaagaagaa
     1261 tctcatttca accttgcttg taacgtcctt tctatttgcg agtgccctcg tagcagttag
     1321 cggtcgtacg attgaaggcg aagtaccggc accgccggcg atcggttcca caattgcaga
     1381 tttcagtttg cctgacggca acggcaaaga acatacgctt gcttcgctgg cgggtaagaa
     1441 tggctcagtc ctgatcttta tggctgtcca atgtccggtt tccaatgctt ataacgagcg
     1501 gatggagaag ttggcgcagg attaccaggc gaagggcatc aacgtgattg gtatcaactc
     1561 gaatgtcgcc gaatcggcag acgcagtgaa agctcacgcg gctgagaagc acctgacctt
     1621 cacgattttg aaagatgcgg gaaacaaagt tgccgaccgc ctcggcgcca gcgttacgcc
     1681 ggaagcctat ttcctcgatg ctgcaaataa gctgctctat cggggacgca tcgacaactc
     1741 gaagaacgcc gatggggtga ctgccagcga attgcgtgac gccatagatg caacgctcgc
     1801 cggcaaaccg atcacaaaga caactgccaa tgcctttggt tgcaccatca agcgtgctta
     1861 ggcagatgaa gctgactcgc aaactgctct ggctgggcct ggtgctgagt ttcatgctca
     1921 gcgccagtcc ggccgatgcg caacgaaagc agacctcaaa gaaatcaccg gcgaagatct
     1981 cagtagcggc tatcgatgcc gatggcctgg ttgcgctgct gaagcgcaac gcgacaaaga
     2041 acaaacctct ccttgttaat ttttgggcca cctggtgcga tccctgccgc gaagagtttc
     2101 ccgacctggt gaagatcgac aagcagtacc ggccgcaggg actcgatttt actgccgtct
     2161 caatcgacga tctcagcgag attaaaacca ctgtgccgca gtttctgcgc gaaatgcagg
     2221 tcaccgttcc gacctacctg ttgaatgtca gtgatcccga agtggcgatg agtagagtgg
     2281 atccccaatg gagcggtgcc ttgccggcaa catttctcta tgacgcacag ggcaatcttg
     2341 tctacaagcg gctaggaaga attaaggttg a
//
LOCUS       DASUEX010000371         3814 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_168078, whole genome shotgun sequence.
ACCESSION   DASUEX010000371 DASUEX010000000
VERSION     DASUEX010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3814
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168078"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2872
                     /locus_tag="VFV61_06210"
     CDS             <1..2872
                     /locus_tag="VFV61_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900108.1"
                     /translation="LDFPNVNLTVNDTTHVDLVMRITVQAEVIVNPGAEALVNTQTSE
                     LSYLVTEQAIRDLPLNGRNYTDLAFLQPGVIPYAHRDGGSVVAHGLAMSINGQDPRSN
                     VYLLDGTPQNDFTNGPAGSAASTVLGVETIREFRVESNAYGAEFGRNSGGQINVVSKS
                     GTNDYHGSLYLFHRNDNFDARNFFDRLRKPEFKRNQFGGSVGGPIRKDKTFFFVGYES
                     LRENLGQTISTVVPDLAARSGNIPGGAVVVNPSVRPYLDEYPLPNGANLGGGLAEYNF
                     GFNRTITEHFTQGRVDRNWSDRHQSFARYTFDNANQALPTDFPQFPRAFRSRNHFFTA
                     EHRYIQSERTIHTSRFSFARTRVGQDVDSNTTQTLQPFIPGRESLGNIDIGGMPRFGP
                     QTSVSVKLTQNLFGFEQGMVHTRGRHLIKAGGLIERYQDNMVNPTFSLGIWTFANLRN
                     FLTNVPQSFLGLTPNGALDRYWRFTLFAGYVHDTIRVHPRLTVNAGLRYETTTMPEDI
                     YGRDSALPSLTDTAPTVGPLYENPTHKNISPRFGFAWDVFGDGKTSVRGGYGIYFNTN
                     NQQNLIVTVTNPPATPRIAVQFTPTSTCPRSFPAAPLGCPFANSIRPVQFDLDNPYLN
                     VYNFSVQRELPWDTVVTLGYAGSRGIHLLRSNDVNTAVPVINADGTPFYPLNAPRRNT
                     AFSTIELKSSDGDSWYNAMIFEVRKRWNRDFNIQSSYTWSRNIDTTQASTFFSDATNG
                     TTTAFPEIPGLNYNKGLADYHAKHNWVVNFTWDLPFARNSRGLRKTLLDGWQLAGIGN
                     ARSGNPLTVFVQANRSRSLWQPSLGPGIGRDRASMAPGFTYETAVLGGPDQYFNPLAF
                     VVPPAGTLGNIGRGAFIGPNLRTFDLAAVKKTRLSGLGDSASLQIRVEAFNLFNRANF
                     APPALTAFAGAAANETALSTFGRIRSTVTSARQIQLGIRLAF"
     gene            3053..3502
                     /locus_tag="VFV61_06215"
     CDS             3053..3502
                     /locus_tag="VFV61_06215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900109.1"
                     /translation="MLWIVFALGAALSWGLYGPALHRGQVALGSPMRALLCVGVAYFL
                     IGVLVPLAMLSYQGELGGFTMKGSLAATLGGTLGAVGAVCIIFAFRSGGLPTYVMPLV
                     FAGAPLVNVLFSMWLHPPKTMPNPLLYLGFLLAAVGAGMVLYFKPPA"
     gene            3565..>3814
                     /locus_tag="VFV61_06220"
     CDS             3565..>3814
                     /locus_tag="VFV61_06220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900110.1"
                     /translation="MRHLKVRTIGSIVFVILLSVGEAAAQAGGNWPQWRGPNRDGIST
                     ETGLLKQWPENGPPLAWKAAGAGFGYSSFAVVNGRLYTM"
BASE COUNT          799 a         1069 c         1041 g          905 t
ORIGIN      
        1 gctcgatttt cccaatgtga accttacagt caacgacacc acccacgttg acctcgttat
       61 gagaataacg gttcaggctg aggtaatagt caaccccggt gccgaagccc tggtgaatac
      121 gcaaacatcc gaactgagtt acctggtaac cgaacaagcg attcgcgatt tgccgttgaa
      181 cggccggaac tataccgacc tggcgtttct ccagccgggc gtaattccct acgcgcatcg
      241 cgatggcggg tcggtcgtgg cccacggttt ggcgatgagt attaacggac aagatccacg
      301 ttcgaacgtt tacctgctcg atggtacgcc gcaaaacgac tttacgaacg gaccggccgg
      361 aagtgccgcg agcaccgtgc tgggcgtgga gacgatccga gagtttcgcg ttgaatctaa
      421 tgcttatggg gcagagtttg gacgcaattc tggtggccag ataaatgtcg tcagcaagtc
      481 tggcactaac gactatcacg gcagtctcta tctctttcat cgcaacgaca atttcgatgc
      541 gcgcaatttc ttcgaccggc tgcgcaaacc tgagttcaag cgaaatcagt ttggtggcag
      601 cgtcggcgga ccgatccgga aagacaagac tttctttttt gtcggttatg agtcactacg
      661 ggagaatctg gggcagacga tctctactgt tgttcctgat ttagcagccc gcagcggaaa
      721 tattcccggc ggtgcagtcg tcgtcaaccc ttctgtgcga ccatacctgg acgagtatcc
      781 tttgccgaac ggagcaaatc tgggcggcgg actcgccgag tacaacttcg gtttcaaccg
      841 gacaattacg gagcacttca cgcagggtcg tgtcgatcgc aattggagcg atcgccacca
      901 gtcgtttgcc cgttacactt tcgataatgc caaccaggca ctgccgacag actttccgca
      961 gtttccccgc gctttcagat cgcgaaatca tttttttacg gccgaacatc gttacatcca
     1021 aagcgaacga acgattcata catcgcgctt tagttttgcg cgcacacgcg tgggccagga
     1081 cgtcgacagc aataccacgc agactctgca accgtttatt ccagggaggg aatcactcgg
     1141 taacatcgat attggcggta tgcctcgctt cggtccgcag acatcagtca gcgtgaaact
     1201 gacccagaac ctgtttggat ttgaacaggg aatggtgcac acccgcgggc gccatttgat
     1261 caaggcaggc ggactgatcg agcgctacca ggacaacatg gtgaatccca cgttctccct
     1321 tggcatttgg acgttcgcca acttgagaaa cttcctgacg aatgtcccgc agagctttct
     1381 cggactaacc ccaaatggag cgctggatcg ctactggcgt ttcacgctct ttgccggcta
     1441 tgtgcacgac accatacgcg tgcatccgcg tctaaccgtc aatgccggac tgcgttacga
     1501 aacgacaaca atgccggaag atatctatgg acgcgattcg gcgctgccgt cattgactga
     1561 taccgcgccc acggtcgggc cgctttacga gaatccgacg cacaagaaca ttagcccgcg
     1621 gtttggtttt gcctgggacg tgttcggcga tggcaagacg agcgtgcgtg gcggttacgg
     1681 catctacttc aacacgaata atcaacagaa cctgattgtc acggtgacaa atccgccggc
     1741 cacaccgcgg attgccgttc agtttacgcc gacctcaacg tgtcctagat ctttcccggc
     1801 agcgccgctc ggctgcccgt ttgcgaattc gatccggccg gtacagtttg atctggataa
     1861 tccctatctc aacgtctaca acttcagcgt gcagcgcgag ctgccctggg ataccgtcgt
     1921 cacgctagga tatgcgggtt cacgcggcat ccacctgttg cgcagcaacg atgtgaatac
     1981 cgccgtcccc gtcataaacg ctgacggcac tcccttttat ccgctgaatg cacctcgccg
     2041 caacaccgct ttttcaacga ttgaactgaa gagcagcgat ggtgattcct ggtacaacgc
     2101 catgattttc gaggtgcgca agcgttggaa tcgcgacttt aacattcagt cgtcttacac
     2161 ctggtcgcgc aacattgaca ccacacaagc gtcgactttc ttttccgacg ctaccaacgg
     2221 gaccacgacc gcatttccgg aaatacctgg tttgaactac aacaagggcc tcgcggatta
     2281 ccatgcgaag cacaactggg tggttaactt cacctgggat ttgccattcg cgcgaaacag
     2341 tagagggttg cgcaagactc tgcttgatgg ctggcagctg gccggaatcg gcaatgctcg
     2401 cagtggtaat ccgttgacgg tattcgtgca ggcaaatcgc tcgcgttcgc tgtggcagcc
     2461 ttctctcggt cccggcatcg gacgcgaccg cgcgtcgatg gcgccagggt tcacttatga
     2521 aacggcggtg ttaggcgggc ctgaccaata ttttaatccg ctcgcgtttg tggtcccgcc
     2581 cgctgggacg ctcggcaaca ttggacgggg agcgtttatt ggtcctaact tgcgtacgtt
     2641 tgatctagct gccgtaaaga aaacgaggtt gagtgggtta ggagattcag cgagtctgca
     2701 aatccgcgtg gaagcgttca atctcttcaa ccgcgctaac tttgcgccac cggctttgac
     2761 ggcgttcgct ggtgcggctg ctaatgaaac ggcgctcagt acgtttggac gcatccgctc
     2821 aaccgtcacc agcgcgcggc agattcagtt aggaatcaga ctggcctttt aaacgattgc
     2881 cgattggcga ttggcgattg ccgattggtc tcttgagcga ggcaacggtg atctatgagc
     2941 catatcactc gctaagcaat cttcatttca catcggcaat cgccaatcgg catttggcaa
     3001 tcagcaatcg gcaatcgcta atcggcaatc gccaatctca ggaggtctcc ctatgctttg
     3061 gatcgttttt gcactcggcg cggccttgtc atggggtctg tatggacccg cgctgcaccg
     3121 cggacaagtt gctcttggta gtccgatgcg cgcactcttg tgcgtcggcg ttgcttattt
     3181 cctaatcgga gtcttagttc ccctggcgat gctttcgtat cagggcgagc ttggcggctt
     3241 tacgatgaaa ggttcgttgg cggcaactct cggcggcacg ctcggcgcag tcggtgcagt
     3301 ttgcattatc tttgcctttc gtagcggcgg cctgccgacg tatgtcatgc ccttagtatt
     3361 cgccggagca ccgctggtga acgtgctttt ttcgatgtgg ctgcatccgc ctaagaccat
     3421 gcccaacccg ctactctatc ttgggtttct gttggctgcg gttggcgccg gcatggtgct
     3481 ctacttcaaa ccgccggcgt agtttttgtt gttgctgttc tatctctccc cgcttccgtt
     3541 cttttactaa aggagatccc ggaaatgaga cacctcaaag tgcgaactat cggcagcatc
     3601 gtgttcgtga ttctgctatc tgttggcgaa gccgcggcgc aggcaggcgg caattggccc
     3661 cagtggcgcg ggccaaatcg cgatggcatt tcaactgaaa cagggttgct aaagcagtgg
     3721 ccggaaaacg ggccgccgct cgcctggaag gcagctggag cgggattcgg atactcctcg
     3781 ttcgcggtag ttaatgggcg cctctatacc atgg
//
LOCUS       DASUEX010000372         3190 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1106755, whole genome shotgun sequence.
ACCESSION   DASUEX010000372 DASUEX010000000
VERSION     DASUEX010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3190
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1106755"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(27..2729)
                     /locus_tag="VFV61_06225"
     CDS             complement(27..2729)
                     /locus_tag="VFV61_06225"
                     /inference="COORDINATES: protein motif:HMM:NF018190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding family 9 domain-containing
                     protein"
                     /protein_id="HEX4900111.1"
                     /translation="MKRIVLLSALIVLFAVNALAQSNGSTPTPAPPSSPAPSPALVSA
                     SPSLNPPGTETSSTTDTKAAATVAAPAAKGAVTLPPEKANPVTIPKFEKPPTIDGVLN
                     DPVWQSAAVLKDFYQIDPGDNIAPSKPTEVLIGYDSRFLYIAFKAFDEQDKIRSTVAK
                     RDNIWQDDYVGFYLDTFNDKRRAFELFFNPLGIQGDGILTEGRGEDMSIDLLLDSKGT
                     ITSEGYFVEIAIPFKSLRYEAGKGKLWGAHFFRRIQRFNRELDSWMPFSRSIDGNMNQ
                     AGHLGGLEGISVERTIELIPSLTLSQDGRFVRTFGVFPNTPAAATDPGRIVNQPLAFD
                     PGLTAKFIPSPAITVDLAINPDFAQVEADQLVVTANQRFPIFFPEKRPFFLEGIDVFT
                     TPVTAVHTRSIVDPDVAIKTTGKIGRTSFGLIAASDNGPGNLSLDDRGALNVCRDRRA
                     LDPRVVCNNERFEDKNAHIGVLRLKRDIGKENSLGILATSYNFIEKHNQLVAIDGRFR
                     LDKQTTLTFQTLGTTSRNFFFDPNEGKTRYRTGNGFGYSYDYNVSGRNWGYELFGEGF
                     TKDYRADVGFFQRQNSNFNFAFVRYNSDPKPKQKIISYHIHNASHIDYDWQGRMYTWE
                     SENLVELSLPRSSWLGVWYEPAYERLFDHEFGPTREAKQCNPFGLPNPCTFFGESNER
                     SVMKQHISGYFGSNFNKKIQFNGQVTYRVNHLDLDFGNLPKYPRASPPAVRARELAAA
                     GRCTDTDPNDGIDARDPGCVAPQDPGAGKLLQMNGSITFQPTNEFRTSISLNKQRLVR
                     NDTGLTAFDVNIWTWRGTYQFTKSYFARAIVDYNTLNSRVRAQFLTGWTPSPGTSFYI
                     GYNDDVNYASAHPFTSQIVPGFRRNSRTFFVKMSYLIRRSFGGN"
     gene            complement(2995..>3190)
                     /locus_tag="VFV61_06230"
     CDS             complement(2995..>3190)
                     /locus_tag="VFV61_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900112.1"
                     /translation="SISLAVPANSDFTIETDADDVDNSGLTVVEDIATSKRMRRWKVG
                     RGGNVFVLSTGDGRVALRSR"
BASE COUNT          737 a          825 c          887 g          741 t
ORIGIN      
        1 cgacacccga catctgccat ctgattctag ttcccgccga agctcctccg aatcaagtaa
       61 gacatcttca cgaagaacgt gcgtgaatta cgcctgaagc cgggaacaat ctgcgacgta
      121 aacggatgag ccgaagcgta gttcacgtca tcgttgtaac caatgtagaa cgacgtgcct
      181 ggacttggtg tccagccggt taggaactgc gcacgcaccc gcgagttaag cgtgttgtag
      241 tcaacaatcg cgcgggcgaa ataggacttc gtgaactggt acgtaccgcg ccaggtccag
      301 atgttgacat caaacgcggt cagtccggtg tcgttgcgca ccaaccgctg cttgttaaga
      361 cttatcgaag tgcgaaactc gttcgtaggc tgaaaagtaa tgctgccatt catctgcaat
      421 agcttgccgg cgcctggatc ctgaggtgcg acacatccag gatctctcgc gtctatgccg
      481 tcgttggggt cggtatcagt gcaccgaccc gcggctgcca actctctggc cctcaccgct
      541 ggcggactcg ccctcgggta cttgggtaga ttaccgaagt caagatccaa atgattgacg
      601 cgataggtaa cctgcccatt gaactgaatc ttcttgttaa agttcgagcc gaagtaaccc
      661 gagatgtgct gtttcatcac agatcgttca ttgctctcac caaagaaggt acaagggttc
      721 ggtaagccga acgggttgca ttgcttagcc tcgcgcgtcg ggccaaactc gtgatcgaag
      781 agtcgctcgt acgccggctc gtaccacaca ccaagccaac tgctgcgtgg cagactcagc
      841 tcgaccagat tctcactctc ccaggtatac atgcgtccct gccagtcgta gtcgatgtgc
      901 gaggcgttgt gtatgtgata ggagatgatt ttctgcttcg gcttaggatc cgagttatag
      961 cgaacaaagg cgaagttgaa gttagaattc tgccgctgga agaacccaac gtcggcacga
     1021 tagtccttcg tgaagccttc cccgaagagc tcgtagcccc agttacgacc agaaacgttg
     1081 taatcgtatg aataaccgaa gccgttaccg gtccggtagc gcgtcttccc ctcgttcgga
     1141 tcgaagaaga agtttcgcga tgtggtaccg agggtttgaa aggttagcgt tgtctgcttg
     1201 tcgagccgga aacgaccgtc aatcgccaca agctgattat gcttctcgat aaaattgtag
     1261 ctggtcgcca gaatgcctaa tgagttttct ttgccgatgt cgcgtttaag acgtaacacg
     1321 ccaatatgag cattcttgtc ctcgaagcgt tcgttgttgc atacaactcg cggatcgagc
     1381 gcacggcggt ctcggcaaac gttcagcgcg ccgcggtcgt caagactcaa gtttcccgga
     1441 ccattgtcgc tcgccgcaat taatccaaag ctcgtgcgtc cgatcttgcc ggtagtcttg
     1501 atagcaacgt cgggatccac gatcgaacgc gtgtgcactg cggtaaccgg cgttgtaaag
     1561 acatcgatgc cttccaggaa gaagggtctc ttttccggaa agaaaatagg aaagcgttgg
     1621 tttgcggtaa ccactaactg atctgcctca acctgcgcga agtcaggatt gatagcgagg
     1681 tcgaccgtga tggcgggact gggaataaac ttcgcagtca aaccgggatc gaacgccagc
     1741 ggttgattga cgatgcgacc gggatcagta gcagctgccg gagtgttagg aaacacgcca
     1801 aaggtgcgaa caaatctgcc gtcctgcgaa agcgtcaggc tgggaattaa ctcgatagtg
     1861 cgctcaactg aaattccttc gaggccaccg aggtgacctg cctgattcat gttgccgtcg
     1921 atgctgcgag agaacggcat ccatgaatcc agctcccgat tgaaacgctg aatccggcgg
     1981 aagaaatgcg ccccccagag tttgcctttg ccggcttcat agcgcagcga cttaaagggg
     2041 atcgcaatct ctacaaaata accttcagag gtaatcgttc cttttgagtc gagcagcaag
     2101 tcgatactca tgtcctcgcc gcggccttca gtcaaaatgc catcgccttg aatgccgaga
     2161 ggattgaaga aaagctcgaa ggcgcgacgc ttgtcgttga aagtgtctag atagaagcct
     2221 acataatcgt cttgccagat gttgtcgcgc ttggccacgg tcgagcgaat cttgtcttgc
     2281 tcatcgaagg ctttgaaagc tatgtaaagg aacctggagt cgtaaccaat cagaacctca
     2341 gtgggcttcg atggcgcaat gttgtcgccg ggatcgattt ggtagaagtc cttgagcacg
     2401 gcagcgctct gccacacggg atcattgagc acaccgtcga ttgtcggggg cttttcaaac
     2461 ttgggaatag ttactggatt tgctttttct gggggcaagg tgactgcgcc tttggcggcc
     2521 ggcgcagcta cggtggccgc ggctttcgtg tctgtagtcg aactcgtctc agtgcccgga
     2581 ggatttaggg acgggctagc agagaccagc gctggggatg gcgcgggtga cgacggcggc
     2641 gccggggtag gcgtactacc gttagactgc gccagcgcgt ttaccgcgaa gagcacaatg
     2701 agagcgctga gtaaaactat tcttttcata atgatctctt taaattgaac ggttcggttg
     2761 ccgttagcct ggatcattag acccaacact gacattcgtg cttttcagaa ccggcgattt
     2821 cgggggttaa ttgggcctgg ggagagaccc aactaatggc gcaaaagtaa atcgagatta
     2881 gccaggatgc gcgggcgcta tattgtttac gcccgcggcc tggttttgta aaaactgatt
     2941 tagattgttt cgcacgcgag aagttgcctg tcgggtttgg aatccaatgt gaccttaacg
     3001 tgaacgcaac gctacacgac cgtcgccggt gctcaacacg aagacgttgc cgccccgtcc
     3061 tactttccaa cggcgcatcc gttttgaagt tgcaatatct tccaccaccg tcagaccgct
     3121 gttgtcgaca tcgtcggcgt cagtctcaat ggtgaaatcc gagtttgcgg gaacggccag
     3181 tgatatcgag
//
LOCUS       DASUEX010000373         3794 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_74909, whole genome shotgun sequence.
ACCESSION   DASUEX010000373 DASUEX010000000
VERSION     DASUEX010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3794
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74909"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /locus_tag="VFV61_06235"
     CDS             complement(<1..620)
                     /locus_tag="VFV61_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900113.1"
                     /translation="MYQQIPTVKFDAGTFVPQFAPEMFQALRLTNEAEAEVLKFLAER
                     PLHTIILTGFIRDNGLVSPANRGTFYGVRNTKGELEGVALIGHATLMETRTERAVWAF
                     AEVAQRCSSTHMILGEQERVEEFWSYYQAGGQQMRLDCRESLFELRYAGKPDQEVKGL
                     RLATIAELELIVPIQAQMAEEESGINPLERDAEGFRQRCQRRIEQGR"
     gene            complement(669..2660)
                     /locus_tag="VFV61_06240"
     CDS             complement(669..2660)
                     /locus_tag="VFV61_06240"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX4900114.1"
                     /translation="MKPTTRSSLTGITSLALVFSLCCGIMITDNAHAASHGRNSGEGK
                     QHQKLAQDLLDQARGAAPEETLKVIVQLNGTMSGELNGLLRSNGVKVKKHFAALNALA
                     IELPPSVVESLNQFEEIEFVSVDSEMRSFGGHVAHTTGADNVRNMATSGALDGSGVGI
                     AIIDSGIYPAHVAFLEAAGSTRSRIVKSVDFTGENRTDDPYGHGTHVASAAAGNGLVS
                     GGKYIGIAPKANLVNLRVLNSQGLGTVSNLLGALDWLLANRSIYNVRVANISLGMPAI
                     NSYRLDPVCVAVRRLVDAGVVVVAAAGNNGKNGAGQKIYGQVHSPGNEPSAITIGAAD
                     TKGTDNRADDSIASYSSRGPTRSYRVDVYGRKHYDNQAKPDLVAPGNKLIFAQSPNCL
                     LVQQDPTLDAAASPNVYRDQMKLSGTSMAAPLAAGAAALMIQANPKLTPNMTKMILMY
                     TAQQLAGFNMFEQGTGELNIDGAVRVAKLVRQDLTYPSLGTSLLTASAPYPTSTITYG
                     SSSYTFTWAQGITMGSTFATGTSLITRYQRVYAQGVLIGDGVLIGDGVLISDGVLIAD
                     LTKLTGVLIGDSILTSYGTTLGGGGFFLSSGVLLGDGVLLSDGVLLSDGVLLSDGVLL
                     ADGVLLSDAIVQANLALIAGDNTNSMPSPEDSGVDYTGF"
     gene            2876..3724
                     /locus_tag="VFV61_06245"
     CDS             2876..3724
                     /locus_tag="VFV61_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900115.1"
                     /translation="MAKILALPEAIESLRDRAWRREAESRVEDRAAAESFIEEVGFCS
                     ALTDRRQPGPSLYIAVCGRRDAHLPRNVQKDPECRLAWTIKDEVIRRGRIYYGKLARA
                     RTTFIAPGLITHFNAIWGVPRDREATTLSDGARAVLKVLRREWEMGTRDLREASGIAS
                     RPQFNKALDELQKTFKVIPSEVIYEPGFTYIWSLSEARFPAELRKRANREVALREIAR
                     AYLAGAKLTLAGELSRVTGLSRVDAGLGNWALVDEGFATRCAPGVYCLAELAQWAEKE
                     LRRRPV"
BASE COUNT          815 a         1026 c         1079 g          874 t
ORIGIN      
        1 cgcccctgtt cgattcgccg ctggcaacgt tgtcgaaagc cttcggcgtc ccgctccaga
       61 ggattgattc cactctcctc ttcggccatt tgcgcctgta tcggaactat cagttcaagt
      121 tctgcgatcg tagcgagtcg caggcccttt acttcctggt caggttttcc agcataccgc
      181 aactcaaaca gagactcccg gcaatcaagg cgcatttgct ggccacccgc ctggtagtaa
      241 gaccagaact cttcaacccg ttcctgctcg cccaggatca tatgagtgga ggaacaacgc
      301 tgggcaactt cagcaaacgc ccacacggcg cgttcagttc gagtctccat caatgttgcg
      361 tgaccaataa gcgcgacacc ttcaagctcg cccttggtat tgcgaacacc atagaaggtg
      421 ccgcgattgg cgggcgaaac cagaccgttg tcgcgaatga agcccgtaag aataatggtg
      481 tgcagcggac gctccgcgag aaacttcaag acctcagcct ccgcttcgtt cgtgagcctc
      541 aaggcctgga acatctcagg tgcaaattgc ggtacaaagg tgccggcgtc gaatttcacc
      601 gtggggattt gttgatacac gtctttatcc tcttaatggt tgaaggttaa ggtctcttgt
      661 tgcggaactc agaaccctgt gtaatcaact ccagaatctt ctggcgatgg catactgttg
      721 gtattgtccc cggcaattaa cgccaggttt gcctgaacga tcgcatcgct tagcagcacg
      781 ccgtcggcta gtaggacgcc gtcgctcagt aacacaccgt cactcaacaa cacgccatcg
      841 cttaacaata cgccgtcccc aagcagcact ccgctgctga ggaagaagcc gccgcccccc
      901 agagtcgtgc cgtagctggt tagaatgctg tcgccaatca gaactccggt gagtttcgtg
      961 aggtcggcta tcaaaacgcc gtcgctaatt aggacgccgt cgccaattag gacgccgtcg
     1021 ccgatcagca cgccttgcgc atagaccctt tgatacctgg taatcagact ggtgccggtg
     1081 gcaaaggtac tgcccattgt gatgccttgg gcccaggtaa acgtgtaact cgacgaacca
     1141 taggtaatag tggaggtggg gtaaggagca gaggcggtta gtaaagaggt accgaggctc
     1201 ggataggtca gatcctgtcg aactaacttg gccacacgga ccgcgccgtc aatattcagc
     1261 tcgccggtgc cttgctcgaa catgttgaat ccggcaagtt gctgcgccgt gtacatgaga
     1321 atcatcttcg tcatgttggg agtgagcttg ggatttgcct gaatcattag ggctgcggct
     1381 ccggcagcga gaggcgctgc cattgaggta ccgctgagct tcatctgatc ccggtagaca
     1441 ttgggactgg cagcagcatc aagtgtcggg tcctgctgta ccagcaaaca gtttggtgac
     1501 tgtgcaaaga ttaatttgtt tccgggagcg actaagtcgg gcttcgcctg gttatcataa
     1561 tgcttccggc catagacatc gacgcggtag ctgcgcgtgg gaccgcgcga actgtaactg
     1621 gcaattgagt cgtcagcccg attatcagtg cctttcgtat cggccgcacc gattgtaatc
     1681 gctgaaggtt cgttgccggg cgagtgaact tgaccgtaga tcttctgacc ggcgccgttc
     1741 tttccgttgt ttccggcagc agcaacgacg accactcctg catccactaa acggcgcact
     1801 gctacgcaaa ccgggtccag gcggtaagaa tttatcgcgg gcatccctaa gctgatattc
     1861 gcaacgcgaa cattgtaaat gctgcgattc gcaagcaacc aatcaagggc gcctagcaga
     1921 ttggagactg tccccaaacc ctgagaatta agcacacgaa gatttacaag gttggctttg
     1981 ggagcgatcc caatgtactt tccgccagat accaaaccgt ttccggcggc tgcggacgcc
     2041 acgtgagttc catgaccata cgggtcatcg gtacgattct cacctgtgaa atcaacactc
     2101 ttaacaatgc gcgagcgcgt gcttccggct gcttcaagaa acgcgacgtg agctgggtaa
     2161 atgccggaat cgatgatcgc gatgccgacg ccactgccgt cgagggcgcc gcttgtggcc
     2221 atgttgcgta cgttatcggc tcctgtggtg tgggccacat ggccaccgaa agaccgcatc
     2281 tcgctatcaa ctgagacaaa ctcgatttct tcgaactgat taagtgattc aacgacgctg
     2341 ggaggaagtt ctattgcgag ggcattcaaa gcggcaaagt gcttcttgac cttaacgcca
     2401 ttgcttcgca gtaggccatt gagttcgccg ctcatggtgc catttagttg cacgatgact
     2461 ttcagcgttt cctcgggcgc ggctccgcga gcttggtcaa gaaggtcctg agccagcttt
     2521 tgatgctgct tgccttcgcc gctgtttctg ccgtgcgagg cggcgtgggc gttgtcagtt
     2581 atcatgatgc cgcagcagag cgagaagacc agtgcgaggc tggtgatccc ggtgagcgac
     2641 gatcttgttg tgggcttcat atcaagtgtt cctcagactc atcagttcga cttgccggag
     2701 cgatgacacg ccttcggcac ccctgatttg gcaacggtta tgccagtagt ccttttcgct
     2761 caataacgcc tgttttgttg ggactttgcg tgattttggt ctaactcgtg gttgctgctg
     2821 gggtgaccgg ttcggtcagg ctgatgaccg aaccttgtca agatgaagga ttgaggtggc
     2881 taagattctt gctcttcctg aagcgataga gagtctccgc gaccgggcct ggcggcgtga
     2941 ggcggagagc cgtgtcgagg atcgcgcggc cgcagaatca ttcattgaag aggtgggctt
     3001 ttgtagcgca ctaactgatc ggcgccagcc aggtccatcg ctttacatcg ctgtctgcgg
     3061 acggcgcgat gcgcacctgc cacgcaatgt gcagaaggac cctgagtgta ggttggcctg
     3121 gaccatcaag gatgaggtga ttcgccgagg aagaatctac tacggaaagc tggcgagggc
     3181 ccgcacgact tttattgccc ctgggctgat cacccatttc aacgcaatct ggggcgtacc
     3241 ccgggatcgc gaagctacga cgctatcaga tggcgcgcga gctgtactga aggtcttgcg
     3301 gcgtgagtgg gaaatgggaa ctcgcgactt gcgcgaggcc agcggaatag cgagtcgccc
     3361 tcagttcaac aaagctctcg atgagcttca gaaaaccttc aaagtgattc cttcggaggt
     3421 tatctatgag ccaggcttta catatatctg gtcattatca gaggcgcggt ttcccgcgga
     3481 gctgcgcaag agagcaaata gggaagtcgc tttacgggaa atagcccgcg cttacctggc
     3541 tggcgccaaa ctgacactag cgggtgaact ctcccgcgtg accggcttat cgcgagttga
     3601 cgctggattg ggaaactggg cgctggtgga tgagggcttt gccactcgct gcgcgcccgg
     3661 agtgtactgc ctggctgaac tagcgcaatg ggccgaaaag gaactcagaa gacgccctgt
     3721 ctgagcttgc cttaacgatc gagcggtagg ttgatctctt ctgctgcatg tcgctcaagt
     3781 tttgccattt caat
//
LOCUS       DASUEX010000374         3324 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_289229, whole genome shotgun sequence.
ACCESSION   DASUEX010000374 DASUEX010000000
VERSION     DASUEX010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3324
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289229"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="VFV61_06250"
     CDS             complement(<1..307)
                     /locus_tag="VFV61_06250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900116.1"
                     /translation="MSIARRSESHVSQSPTVLMCPPDHYGIAYEINPWMSLSRPSDYL
                     LACQQWRTLYHLLKDRLDVNICLIEPVPGLPDMVFTANAGFAWGEKFIVSNFNHKVRR
                     "
     gene            604..2388
                     /locus_tag="VFV61_06255"
     CDS             604..2388
                     /locus_tag="VFV61_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HEX4900117.1"
                     /translation="MYLRCNRRVKDGKEHRYWNIVESKRCSGGKVVQRQVLYLGEIND
                     SQRESWCRVIEAFDEKTQQRTQLALFPAEKELPEYAEGYGVQVRLEAMELCRPRQFGA
                     CWLACHLYEQLALDQFWKDRLLDSREGSCWRHVLQTLVCYRLIDPGSEWRLHRLWFDQ
                     SAMGDLLGEDYALVEKNTLYRCLDKVLEHKPALFSHLTERWQGLFGAKFDVLLYDLTS
                     TYFESSKPADENDKRRYGYSRDKRSDCVQVVIALVVSPEGFPLAYEVLAGNTSDRTTL
                     KEFLNKIETQYGKAERIWVMDRGIPTEEVLTEMRQSDPPVYYLVGTPKGRLSQLEQKL
                     LALPFTTVRSGVEVKLLAQEQELYVLANSHQRIDKERAMRRRQLRALFKRLKELQAMK
                     FNSSQTLLLKLGAAKGKYPAAWRLLDIALPEAKTGPANVSFSFQLNRQKVREVRRREG
                     RYLLRTNLSGREPAQLWQFYIQLVEIEAAFKNLKDDLQLRPIYHQLQHRIEAHIFVAF
                     ISYCLHVTLKARLRPVAGGLTPRAVLDKFATMRMLDVKFPTTDGRTLILSRYTEPDTE
                     HKLLLEQLKLALPPQPPPRITAGGQRVH"
     gene            2515..2772
                     /locus_tag="VFV61_06260"
     CDS             2515..2772
                     /locus_tag="VFV61_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900118.1"
                     /translation="MKRSVKRAAKTKGFSLKRRRLPMYTAAFRSRKLAATDLVNLYLE
                     RIKAHNGTCVEGDLDPATGLYLGDITGIAGAQAAERAVTLN"
     gene            complement(3123..>3324)
                     /locus_tag="VFV61_06265"
     CDS             complement(3123..>3324)
                     /locus_tag="VFV61_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900119.1"
                     /translation="ARAAKSLDEVQPLVKASVAGRLIAIREEITAAEIKEKTEKDFST
                     VPFETINTNLDHLIEIVRVSQT"
BASE COUNT          785 a          886 c          899 g          754 t
ORIGIN      
        1 cacgcctcac cttatggttg aaattgctta cgatgaattt ttctccccat gcgaagcctg
       61 cgtttgccgt aaataccata tccggtaacc caggcacggg ttcgatcaaa catatgttga
      121 catccagtcg atccttcagg agatgataga gcgtccgcca ctgttggcag gctaagagat
      181 aatcgctcgg acggctcaga ctcatccaag gatttatttc gtacgctatt ccatagtggt
      241 caggaggaca catcagcacc gtcggacttt gcgagacgtg cgattctgac cgacgtgcaa
      301 tggacatctt cgttctgttc tccatggcca cgagaccaca agcaattttg accagaacct
      361 tacgcctcgt gatagtttga accattggcg caattcacgt gccattctcc gacgtaatct
      421 ggtctggtgc gcccgagtaa ggccgctagc ccaactttct caactcgcat cgaaataaga
      481 tttttttacg ggtctgagag tgaaaaagtt ttgtcatttc acggggtgag agtttcaaag
      541 tggctgtagt gtagaccaag tatcttttca agcgcaagtt tttgcgtaca atcaaaggcc
      601 aagatgtatt tgcgctgcaa tcggagagtc aaagacggca aagagcatcg ttactggaac
      661 atcgtggaga gcaagcgctg tagtgggggc aaggtggtgc aacgccaggt gttgtatctg
      721 ggcgagatca acgacagcca acgcgagtcg tggtgtcgag tgatcgaagc cttcgatgaa
      781 aagacgcaac aacgcacgca gctggcacta ttcccggcgg aaaaggagtt gcccgagtac
      841 gccgaaggct atggagttca ggtgcgcctt gaggcgatgg agctttgtcg gccgcgccag
      901 tttggagcgt gctggttggc ttgtcatctt tatgaacaat tggccctcga tcagttctgg
      961 aaagatcgtt tactggatag tcgtgagggt agttgttggc ggcatgttct tcagacgctg
     1021 gtgtgttacc ggctgatcga tccgggcagc gaatggcggt tacaccggtt gtggtttgac
     1081 caaagcgcga tgggagactt gcttggcgag gattatgcac tggtggagaa aaacaccttg
     1141 tatcgctgtc tggacaaagt gctggagcat aagccagccc tgttcagtca tctcactgag
     1201 cgctggcagg gtctgtttgg cgccaagttt gacgtgctgc tctacgatct gaccagcact
     1261 tatttcgagt ccagtaagcc cgccgatgag aacgacaagc ggcgctatgg ctacagccgc
     1321 gacaagcgct cggactgcgt ccaagtggtc atcgcgttgg tggtcagtcc tgaaggcttt
     1381 cccttggcct acgaagtgct ggcgggcaac acctcggacc gaaccacgct taaggagttt
     1441 ttaaacaaga ttgaaaccca gtacggcaaa gccgagcgca tctgggtgat ggaccgcggc
     1501 attcccaccg aggaagtact cactgagatg cgtcagagcg atccgccggt ttattacttg
     1561 gtcggcacgc ccaaagggag actcagccag ctggagcaaa aacttttagc tttgcccttt
     1621 actacggtgc gctcgggggt cgaagtgaaa ctgttagccc aagagcaaga actctacgta
     1681 ctggccaaca gtcaccagcg catcgacaaa gaacgcgcca tgcgtcgccg tcaactcaga
     1741 gcgctgttca aacggctcaa agaactgcag gcgatgaagt ttaacagcag ccaaaccttg
     1801 ctgctcaaac tgggagcggc caagggaaaa tatccggcgg cctggcgcct gctcgacatc
     1861 gccctacccg aagcaaagac cggcccggca aatgtcagtt tctctttcca actcaatcgg
     1921 caaaaggtga gagaagtacg ccggcgcgag ggccgctatc tgctacgcac caatctgtct
     1981 ggccgggagc cggcgcaact gtggcagttc tacattcaac tggtcgagat cgaagcggcc
     2041 ttcaaaaact taaaagacga tcttcaattg cggccgattt accaccagct tcaacaccga
     2101 atcgaagcgc atatctttgt ggcgtttatc tcttattgcc tgcacgtcac gctcaaggcc
     2161 cggctacggc ctgtggcagg tggactgacg cctcgcgcgg tgctcgataa gtttgccacc
     2221 atgcggatgc tcgatgtcaa gtttcccacc accgacggcc gcactttgat tcttagccgt
     2281 tacactgagc ccgacaccga gcacaagctt ctcttagaac aactcaagct cgctttgccg
     2341 ccccaaccgc caccccgaat cactgccggc ggccaacggg tacactaaca aaatctagcc
     2401 cgatgtagtg gagacctctt gactgtgagg gttgttttta ttcgcttttt agatcgatta
     2461 cccttccagt tgggaaggat gggttagcag tgaagcgctg cgatcactgc gaagttgaaa
     2521 cgttcagtta agcgtgcggc gaaaacaaag ggcttcagct tgaaaaggcg acgattgccg
     2581 atgtacacag ccgcttttcg ttcaagaaag ctggccgcaa ccgatctcgt gaatctctat
     2641 ctcgagcgca tcaaagccca taacggcact tgcgtcgagg gtgatctcga tcctgccacg
     2701 ggtttgtact tgggagatat tacggggatc gctggcgccc aggcagccga acgcgccgtt
     2761 actttgaatt aaaacgaaga caaacgcatc aagctcggtt cccaagaagc tgaagcacac
     2821 gcacaccgga cccgacgacg aaaaattccc cgattggatt ggacccggca catgagctcg
     2881 accggcaagt ttgccggagc gctgcacggc attccgatca cgatcaaaga caactagaca
     2941 ccttcggaat ggcaccaccg cggcggcagc ggcgggcgga ctacgttgac catgaaatcc
     3001 tcataggcgg tttgtggcct acgtcatctc attgaagaaa tcctttctgg ccttggcata
     3061 gtccgatacg ggatttgcac attccatgtt cagcacgccc gctggtatcg attcgactcc
     3121 actcacgttt gcgacacacg cacgatctcg attaaatgat ccagatttgt gttgatcgtt
     3181 tcgaacggca cagtggagaa atccttttcg gttttttcct tgatttccgc ggccgtgatc
     3241 tcttctctga tcgcgatcag tcgaccggcg acgctagctt tgacaagcgg ctgcacttcg
     3301 tcaagagact tcgcggcacg agcg
//
LOCUS       DASUEX010000375         2429 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_510411, whole genome shotgun sequence.
ACCESSION   DASUEX010000375 DASUEX010000000
VERSION     DASUEX010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510411"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1056
                     /locus_tag="VFV61_06270"
     CDS             <1..1056
                     /locus_tag="VFV61_06270"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HEX4900120.1"
                     /translation="AHRPEVSLGEIESLDLPALQRDLGGDTALVEYFTLDDELLAFVV
                     TNDGIEVVRELCNEAQVSGELQQLRFQIDALRHGSAAIRRHLSELNKRAQHHLQSLYD
                     LLLRPIEETIGERRLVIVPHHQLHYLPFQALHNGESYLIERREVSYAPSAIVLQQCRR
                     KETRDLRKAVLIGVADELTPRVHEEIETLAPLFSEAAVLKDGEATVAALRRQAPSADV
                     LHLACHGQFRPDNPLFSSLRLGDAWLTVRDAYSLGLKSALVTLSACETGVSAVSPGDE
                     LIGLARGFFAAGAPTLVISLWTVDDEATTRLMSDFYSALQETRSPATALRTAQMRLLK
                     ERPHIFFWSPFIVVGRW"
     gene            1327..>2429
                     /locus_tag="VFV61_06275"
     CDS             1327..>2429
                     /locus_tag="VFV61_06275"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX4900121.1"
                     /translation="MTFLLGCLLLLMAALFGGGNQLQPVQASGDEEFVAGQVVVKLAA
                     AADLPAVAAQYSLDPNPLDQFGSRPIYRLRITNGVAADTLAAALAIDPRVVFAEPNYL
                     EKPPEGGGNGWSVGNGWSVGGDIGAYQAQWAPQMIQLSVAHLVNRGTGPNGPIKIAVL
                     DTGIDATHPVLAGHLLAGYDFVDNDNDPSEVGSLQLGPYGHGTHVAGLLALIAPEAKI
                     IPIRVLDERGLGNIWVLAEALRYAVDPDDNPATPDGADVINLSLSTLRRTSLLGSVLS
                     KVCDDIPAPGEDDFPAAANPNLVIVAAAGNAGNEILQYPAAENVRGVIAVAASTSVDD
                     LALFSNRGSWVHVAAPGQAILSTVPGGQFGTWSG"
BASE COUNT          521 a          645 c          689 g          574 t
ORIGIN      
        1 gcacatcgac cagaagtttc gctcggcgag atcgaatcat tagacttgcc ggcgctgcag
       61 cgcgatctcg gaggcgatac ggcgctcgtt gaatacttca cactcgatga tgagctattg
      121 gctttcgtag ttacgaatga cggaatcgaa gtcgttcgtg aactttgcaa cgaggcgcag
      181 gtttccggtg aattacagca gttgcgcttt caaatcgacg cgctgagaca tggatcggca
      241 gccatcaggc gacatttatc ggaactaaac aagcgggccc aacatcatct gcaatcgctt
      301 tacgacctct tgctcagacc aatcgaagaa actattggag agcgacgact ggtaatcgtt
      361 ccgcatcacc agctgcacta tctgccattt caggcgttgc acaacggcga gagctacctg
      421 attgaacgtc gtgaagtttc ttatgcgccg agtgccatcg tgctgcaaca gtgccggcga
      481 aaggagactc gggatttaag aaaggcagtg ctgatcggcg ttgcggatga attaacgccg
      541 cgcgttcacg aggagatcga aactctcgcg ccattgttct ctgaggcggc ggtgttgaag
      601 gatggtgagg cgacagttgc ggcgttacgt cgtcaagcac cctctgccga tgtcctgcac
      661 ttagcttgtc acggtcagtt tcggccggac aatcctttat tctcttcatt gcggctgggt
      721 gacgcatggc tcactgtgcg cgacgcctac agcctggggc tgaagtcggc gttggtaacg
      781 ctcagtgcct gcgagacggg agtaagtgca gtttcacctg gagacgaatt gattggcctc
      841 gcgcgcggct tctttgccgc cggggcgcca accttagtaa tcagtctttg gaccgtcgat
      901 gatgaagcta cgactcgttt gatgtccgat ttctacagcg cgttacagga aaccaggtcg
      961 ccagccacag ctctacgaac agcccaaatg cgtctgctaa aagaacggcc tcacatcttc
     1021 ttctggtcgc cattcatcgt ggtgggtcgt tggtaatttg aatcaaccca accctctgaa
     1081 gtgccatctc agccgaatcc ggttcacgat ttgctcctga catctgagat tccctggaaa
     1141 caatgtttcc caaacggaga aaaactgtat tttggccgaa gttcgccgga ctgtacggtc
     1201 gagactttaa ggaattgacg aattagtagc gactggcgag atgaaaccaa ttccctttga
     1261 gccggacgct ttttcttcaa aatttcaaat aaaccaatgg tcgagggtga aatcttgcgc
     1321 cgccgaatga cattcttgct gggttgtttg ctgttgctaa tggccgctct attcgggggc
     1381 ggaaaccagc tgcagcccgt tcaagccagt ggggatgagg aatttgtggc tggacaagtg
     1441 gtggtgaaac tcgccgccgc cgcggatctt cccgcagtcg ctgcgcaata ttccctagat
     1501 ccaaatccgc ttgatcaatt tggctcccgc cctatttatc gcctgcgcat tacgaacggc
     1561 gtcgccgccg atactcttgc cgcggccctg gcaatagatc cccgcgttgt atttgctgaa
     1621 ccgaactatc ttgaaaagcc gccggaaggc ggtggcaacg gttggagcgt tggcaatggt
     1681 tggagtgtgg gtggtgacat cggtgcttat caagcccaat gggctccgca aatgattcag
     1741 ctgtcggtcg cgcatctggt gaatcgtggc actggcccca atggaccgat taagatcgct
     1801 gtgctcgata ccggcatcga cgccacgcat cccgtgctcg ccggtcactt gctggcaggt
     1861 tacgacttcg ttgacaacga caacgatccg agcgaagtcg gaagtttgca gctcggccct
     1921 tacggccacg gcacacatgt ggctggtttg cttgcgctga ttgctcctga agcgaagata
     1981 attccgatac gcgtcctgga cgagcgcggg ctcggcaaca tttgggttct ggcggaagcg
     2041 ttgcgctacg cagttgatcc cgatgacaat cctgctacac cagacggcgc tgatgtaatt
     2101 aatctgagtt taagcacgct gcggcgaacc agtttgctgg gcagcgtact atccaaagtc
     2161 tgtgatgaca tcccggctcc gggcgaagac gattttcctg ctgcggcaaa cccgaacctg
     2221 gtaatcgttg ctgctgcggg aaacgcaggc aatgaaatcc ttcaatatcc agctgcggaa
     2281 aatgtgcgag gcgtaatcgc tgtggcggcg tcaacgtcgg tcgatgatct cgcacttttc
     2341 tcgaaccgtg gttcgtgggt gcatgtcgct gcgccgggtc aggcaatact cagcaccgtt
     2401 cccggcggtc agtttggcac ttggagcgg
//
LOCUS       DASUEX010000376         5884 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_289367, whole genome shotgun sequence.
ACCESSION   DASUEX010000376 DASUEX010000000
VERSION     DASUEX010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5884
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289367"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1005)
                     /gene="rho"
                     /locus_tag="VFV61_06280"
     CDS             complement(<1..1005)
                     /gene="rho"
                     /locus_tag="VFV61_06280"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEX4900122.1"
                     /translation="MLNLATLKDMSISKLTSIAKAMDVPGATGMRKQELIFKVLAAQT
                     EKSGLIFSEGVLETLPDGFGFLRAPEYNYLPGPDDIYVSPSQIRKFDLRTGDTVSGQI
                     RPPKEGERYFALIKVEAINFESPDMAREKVFFDNLTPLYPDEMLKMEVTPENLSARVI
                     DLVTPLGKGQRGLIVAPPRTGKTMLLQTIANSVTQNHPEVTLIVLLIDERPEEVTDMQ
                     RSVNGEVISSTFDEPPTRHVQVADMVIEKAKRLVEHKRDVVILLDSITRLARAHNAVV
                     PPSGKILSGGIDSNALQRPKRFFGAARNIEEGGSLTIIATALIDTGSRMDDVIFEEFK
                     G"
     gene            complement(1424..2005)
                     /gene="yihA"
                     /locus_tag="VFV61_06285"
     CDS             complement(1424..2005)
                     /gene="yihA"
                     /locus_tag="VFV61_06285"
                     /inference="COORDINATES: protein motif:HMM:TIGR03598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HEX4900123.1"
                     /translation="MKVSSTEFIKSAFTEDDWPHDKRPEIAFMGRSNVGKSSVINSLL
                     RVHGLARTSSTPGRTQSLNFFLINGEFSFVDLPGFGYARVPKIIKSTWGEMVTSYLAK
                     RSQLMLSIHIVDSRHEPTKLDLQLHEWLKYSAKPRLIVATKSDKLSNNELRENLGRAK
                     RLFGEESVIAYSARTGHGRAEVWRAIENALSLN"
     gene            complement(2039..2497)
                     /locus_tag="VFV61_06290"
     CDS             complement(2039..2497)
                     /locus_tag="VFV61_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900124.1"
                     /translation="MSKHHDADLILKLYELRREPVMREARNWFFTFNPQKPEDFLDTV
                     TSDKSGYYRMVISYWDMAASLVNNGAIDAQMFNDANGEHLFVYAKMEPFLADLRRENG
                     NPNFLVHLEKVVKEIPNYEEKIEALRARIGKMIAIWQQRAATAQAVAGKA"
     gene            complement(2629..5058)
                     /gene="lon"
                     /locus_tag="VFV61_06295"
     CDS             complement(2629..5058)
                     /gene="lon"
                     /locus_tag="VFV61_06295"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:NF008053.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEX4900125.1"
                     /translation="MEEYSDHSPADVAWYPMVPIRDVVIFPFTKVAFKIGRSGSVRAL
                     EQALAADRTIFLATQHDATVDEPAPEQIYGVGTLGRILQSQKQENGQIKVVVEGRERA
                     TTVRVENTDGAFMALVRRAPVVNEEGTRLDALIQKIGQLVEQHLRLAPDTQTDALQTA
                     LRNQDPSHLADALASQLRISVEDKQGLLELFSSQARLQRLIELLEMEIEKRQLDRTIQ
                     TRVKRQMEKAQKEYYLNEQIKAIHKELGRKDEKAELEDLKKKIEEAGMTEEAKEKAMQ
                     ELHRLEAMPPMSAEGTVSRTYIDWLVSVPWKQKSKEIRDLSKAEEVLHEDHFGREKIK
                     ERILEYLAVRQLVKNPKGSILCFVGPPGVGKTSLGKSIARATGRKFVRLSLGGVRDEA
                     EIRGHRRTYIGALPGQIIQMMKKAGVVNPVLLLDEVDKLGADFRGDPSAALLEVLDPE
                     QNHTFQDHYLDVEYDLSKVFFIATSNVLHTIPPALQDRLEILRLSGYTEREKLEIAKR
                     HLVPKQAESNGLKTEQFEFSDEGLQEIIRHYTREAGVRNLEREIGSCCRKLARKFVSE
                     SATEDLKEIVDAARVREMLGPIKFRQQVMAAESEVGLATGLAWTEIGGEVLQIEATLT
                     KGRGAVQLTGKLGDVMQESAQAALTCIKSRAERLGMSLDFIRKRDLHIHIPEGAIPKD
                     GPSAGITMATAMASALTRAAVRKNVAMTGEITLRGRVLPIGGVKEKLLAAHRMGIDTI
                     IMPRDNEKDLPEVPEEVRNALCINLVETIDEVLALALEDACPTEAAAADQSKAPPLWT
                     TEPPSQGIQTS"
     gene            complement(5188..>5884)
                     /locus_tag="VFV61_06300"
     CDS             complement(5188..>5884)
                     /locus_tag="VFV61_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX4900126.1"
                     /translation="ISKKDENPSITRDVSGEGVQQALLKILEGTVANVPPQGGRKHPH
                     QEFFPIDTTNILFLCGGAFVGLEKVIGRRLRNKSLGFQAEVKSGSHRHAEALAQVQPE
                     DLIRFGLIPEFVGRLPVCGVLHELDEAALIKILTEPKNALVKQYQKKFDFDNVKLKFT
                     EDALKSVARQAVERKVGARGLMMILEELLLDAMYTLPSQKRIKEFVITSEMVERRRAV
                     PGEGLSGVDGAAA"
BASE COUNT         1218 a         1657 c         1547 g         1462 t
ORIGIN      
        1 gcccttgaac tcttcgaaaa ttacatcgtc catgcgcgaa cctgtatcga tcaacgcggt
       61 ggctatgatg gtcagtgacc caccttcttc aatgttgcgg gccgcgccaa aaaagcgctt
      121 cggtcgttgc agtgcgttgg aatcaatacc gccagaaagg atcttgccgc tgggtggcac
      181 caccgcattg tgagcgcgag ccagacgcgt gattgagtcg agcagaatta ccacgtcgcg
      241 cttgtgctca accagccgtt tggccttttc gatcaccatg tcggcgacct gcacgtggcg
      301 cgtgggcggc tcgtcaaatg tcgaagaaat tacttcgcca ttaaccgagc gctgcatgtc
      361 agtaacttcc tcggggcgct cgtcgatcag caggacgata agggtaactt ccggatggtt
      421 ttgcgttacg gagttggcga tggtttgtag cagcatcgtc tttccggtgc ggggtggtgc
      481 tacgatgagg ccacgttgac ccttgccgag gggcgtcacc aggtcaatca cgcgcgccga
      541 aaggttctcc ggagtgactt ccatcttcaa catctcatcg gggtaaagcg gcgtcaggtt
      601 atcgaagaaa actttttcgc gcgccatatc cggcgattca aagttaatgg cttcgacctt
      661 gatgagcgca aagtaacgtt cgccttcttt cggcggacga atctggccgc tgactgtatc
      721 cccagtacgc aggtcaaact tgcgaatctg cgaaggcgat acatagatat cgtcgggccc
      781 gggaagatag ttgtattccg gcgcccgcag gaagccgaaa ccatcgggca gcgtctcgag
      841 cacgccttca gagaagatga ggccgctctt ttccgtttgg gcggccagaa ctttgaagat
      901 cagctcttgc ttgcgcattc cggtggcgcc cggaacgtcc atcgccttcg cgatggaggt
      961 aagcttggag attgacatgt ctttgagcgt agccaggttt aacatcccac cctcttccat
     1021 gaagtcctgg ggttcgcggc cgtctctctc cggagcgtct tctgtggcaa cgcggacatt
     1081 gccgtcgtcg ttgttattcg cgctcgaagc gcgtgatctt ggtctttgtg gcatggtcct
     1141 tctctatctg actgaacggt aaataactaa cctttgatta tggcagcgcc cgttggcgcc
     1201 acaataattc gcgagcgaaa aaagcgctcc tccttaacaa tgactgcagt gatttcgatt
     1261 agggcggatg ttttaccggg catctcttgg atgccgagtt ctgttccgcg cgtggcctat
     1321 agttagacta aggttgacca aaggggcggc tgagaaactt ccaggcttgt ctaccaaatt
     1381 tcctttaact cgtcgactag ctattcccaa taacgactaa aactcaatta aggcttagtg
     1441 cgttttcaat cgctcgccaa acttcagctc gtccatgtcc ggtgcgcgcc gagtaagcta
     1501 ttacgctttc ctcgccgaac aggcgcttag cgcgtcccag gttctctcgc aactcattgt
     1561 tagacagctt atcggatttt gtggcaacaa ttaaccgagg tttggcggag tacttaagcc
     1621 actcgtgaag ctgcaaatcc agttttgtgg gttcgtggcg cgagtctacg atgtgaattg
     1681 atagcataag ctgactacgc tttgcaaggt agcttgtcac catctcaccc caggtcgatt
     1741 taatgatctt cggtacgcgc gcatagccaa agccgggtag atcaacgaaa ctaaactcac
     1801 cattgatgag aaagaagttt aacgattgag tcctgcccgg cgtcgacgat gttcgtgcca
     1861 aaccatgcac ccgcaggagt gagttgatca cgctcgattt accaacattc gagcgtccca
     1921 tgaaggcgat ctccggccgt ttgtcgtgcg gccaatcatc ctccgtaaat gcactcttaa
     1981 taaactccgt gctgcttacc ttcatctggc tacgcgtccg ccatgcggta tcagaccgct
     2041 atgccttacc ggcaacggcc tgagcagtag cagcccgttg ctgccagatc gcaatcatct
     2101 ttccgattcg agcgcggagc gcctcgatct tttcttcgta gttagggatc tccttgacca
     2161 ccttttccag gtgtaccagg aagttagggt tgccgttttc gcgtctcaag tctgcgagga
     2221 acggttccat cttggcgtag acgaacaggt gttcaccatt ggcatcattg aacatttgcg
     2281 catcaatggc tccgttattt acaagcgatg cagccatgtc ccagtaggaa atcaccatcc
     2341 ggtaataacc actcttgtcg ctggtgacag tgtccagaaa gtcttcgggc ttctgtgggt
     2401 tgaaagtaaa gaaccagttg cgcgcttcac gcatgaccgg ctcgcgacgt agttcataaa
     2461 gcttcagaat aagatcagca tcgtgatgtt tactcattgg gatctcccgt gggttggggt
     2521 taacaagtgc aggttgactg acttggtgcg aaacgcagcc cgaaccgctg cctcagctct
     2581 cacgtgagag tgccggtcag cttagcggcg cgtcagcgta aactttgatt agcttgtctg
     2641 aattccttgt gaaggcggct cggttgtcca gagcggcggg gcctttgact gatcggcggc
     2701 agccgcctca gtggggcacg catcttcgag cgcaagcgcc agcacttcat caatcgtttc
     2761 taccagattg atgcagagcg cattgcgaac ttcctcggga acctcaggca ggtccttctc
     2821 attgtccctg ggcatgatga tcgtgtcgat gcccatacga tgggcggcca gaagtttttc
     2881 cttcacccca ccaattggaa gcacgcgacc acgaagagtg atttcacccg tcattgccac
     2941 attctttctt accgccgcgc gcgtgagcgc tgatgccatt gcggttgcca ttgttattcc
     3001 ggcagagggg ccgtccttcg gaattgcacc ttcaggaatg tgaatatgaa gatcccgctt
     3061 gcggatgaag tcgaggctca ttcccaggcg ttccgctcga gacttaatac atgtaagagc
     3121 cgcctgcgct gactcctgca tgacgtcacc cagctttccg gtgagttgca cggcaccgcg
     3181 gcctttggtc agagttgctt caatctgaag gacttcgccg ccgatctccg tccaggccaa
     3241 tccggttgcc agccccactt cactttcagc ggccattacc tgttgccgga acttgatggg
     3301 gccgagcatt tcgcgaacgc gggccgcgtc aacgatctct ttgagatctt ccgtggcact
     3361 ctccgaaaca aacttgcgag cgagtttgcg acaacaagaa ccgatctcgc gctcgaggtt
     3421 ccgaacgcca gcttcgcggg tgtagtggcg gatgatttcc tgcaaccctt catcggaaaa
     3481 ctcaaactgt tcggtcttca agccattact ttcagcttgt ttcggcacta gatgtcgttt
     3541 ggcgatttcc agcttttccc gctccgtgta gccggagagt ctcagtatct ccagccggtc
     3601 ttgcagcgct ggaggtattg tgtgcaatac attcgaagta gcaataaaga agaccttgga
     3661 gaggtcgtac tccacgtcga ggtaatgatc ctggaacgta tggttctgtt ctggatcgag
     3721 cacctccagc agagccgcgc tcgggtcgcc gcgaaagtcg gcacccagct tatcaacttc
     3781 atcgagcagc agaaccggat tcactactcc ggccttcttc atcatctgaa tgatctgccc
     3841 aggcagagcg ccaatgtaag tgcgtcggtg accacgaatc tcggcttcgt cacgaacgcc
     3901 gcccagggag aggcgcacaa acttacgacc ggtggctcga gcgatggatt tgcccaggct
     3961 cgtttttccc actcccggcg gaccaacgaa acagagaatc gaacctttgg ggttctttac
     4021 gagctgcctg accgcaagat attcaaggat gcgttccttg attttctccc gcccgaaatg
     4081 gtcctcatgc agaacctctt cagccttcga tagatcccta atctctttgc tcttctgctt
     4141 ccagggcaca ctgaccagcc aatcaatata agtgcgggaa acggtgcctt cggctgacat
     4201 cggcggcata gcctcaagcc gatgcagttc ctgcattgct ttctcttttg cctcttcggt
     4261 catgccggct tcttcaatct ttttcttcag atcttcgagc tcggcttttt cgtctttgcg
     4321 tcccagttcc ttatgaatcg ccttgatctg ctcattcagg tagtactctt tttgcgcctt
     4381 ctccatttgc cgcttcacgc gcgtctgaat ggtgcgatcc agctggcgct tctcaatttc
     4441 catttccagc aactcaatga gtcgctggag acgagcctgg gaagaaaaca actccagtag
     4501 gccctgcttg tcttccacgg aaattcgcaa ctgcgaggcc agggcatcgg cgagatgaga
     4561 aggatcctga ttgcgcagcg cagtctgcaa ggcatcggtc tgcgtatcag gcgcgagccg
     4621 caagtgctgc tctactaact gtccaatctt ttgaattagt gcgtcgaggc gagtgccttc
     4681 ttcgttaacc actggggcgc gccgaaccag agccatgaag gcgccatcgg tattctccac
     4741 tcgaaccgta gtagcccgct cacgtccttc gactacaact tttatctgtc cattttcctg
     4801 cttctgtgat tgcagaatgc gaccgagtgt tcccactcca tagatttgtt cgggcgctgg
     4861 ctcatcgaca gtggcgtcgt gctgagtggc caggaagata gtccgatccg cagcgagggc
     4921 ctgctctagg gctcgtaccg atccagaccg cccaattttg aaggcaactt ttgtgaacgg
     4981 gaagatcacg acatcacgaa tgggcaccat ggggtaccac gcaacgtcgg ccggagaatg
     5041 atcagagtat tcttccattt ggataaagcc ttttttccgc tgatgcatta agggcgagcc
     5101 gcttgaattg ccgtctcgcc ctttaagtcg tcctgattgt aaatcatttc ctgcggacgc
     5161 gctactaacc tttcagtgcg ctgtgggtca ggctgcggcg ccgtcgacac cgctaagtcc
     5221 ttctcctgga acggcgcgcc gcctctcaac catctcactc gtgatcacaa attccttgat
     5281 ccgtttttgc gatggcaggg tgtacatcgc atccagcagc aactcctcca gaatcatcat
     5341 caggccgcgt gcgccgacct tccgctcaac tgcctggcgg gccacggatt tcaacgcgtc
     5401 ttcagtgaac ttcaatttca cattatcgaa atcaaacttc ttctgatact gcttgacgag
     5461 agcgttcttg ggctccgtca gtatcttgat cagagcggct tcatcaagct catggagcac
     5521 gccgcagaca ggtagccggc cgacaaattc gggaatcaaa ccgaagcgaa tgagatcctc
     5581 cggctgcact tgcgcgagag cctcggcgtg gcgatgcgag ccgctcttca cttcagcctg
     5641 gaagccgagc gacttgttac gcaggcggcg cccgatgacc ttctccagcc ccacgaacgc
     5701 tccgccgcag aggaacagaa tgttggtggt gtcaattggg aagaattcct gatgcggatg
     5761 tttgcgccca ccttgcggag ggacgttggc gacggtccct tccagaatct tcagcaaggc
     5821 ctgctgcacg ccttcgccgc tgacgtcgcg ggtgatcgag gggttctcgt ccttcttgga
     5881 aatc
//
LOCUS       DASUEX010000377         2675 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1443762, whole genome shotgun sequence.
ACCESSION   DASUEX010000377 DASUEX010000000
VERSION     DASUEX010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1443762"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..726
                     /locus_tag="VFV61_06305"
     CDS             <1..726
                     /locus_tag="VFV61_06305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900127.1"
                     /translation="NGLQLEWTKRLSHNLTFQAAYTFSKAIDTTSEATFVGAGDSNQT
                     GNDARSARALSRFHTPHRFTFFGTYRTPWFSNDRGLLGQVLGGWQFSTVVKLAKGTPF
                     TVVTTGVDLNLDGFAESRPILLNPSILGRSVADPATATISLPSSAFRQLTIADRGASI
                     LGRNTFFGDGVRNIDFGIFKNFPMPWEGHRLTLRADMFNAFNHVQYGFPTTDITNANF
                     GRILGLGTLYSPRTIQFSLRYQY"
     gene            749..1993
                     /locus_tag="VFV61_06310"
     CDS             749..1993
                     /locus_tag="VFV61_06310"
                     /inference="COORDINATES: protein motif:HMM:NF014675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HEX4900128.1"
                     /translation="MSSCLKLFLRFSAITLLLSLCSPLLNAQSKSDEVRALWVVRTTL
                     TSPEKIHQLVSSAADNGFNTLIVQIRGRGDAYYNSRVEPRAIELRDQPASFDPLALTV
                     AEAHKRGLKVHGWINTNLLANLDALPVQPDHVYNKHPEWLAVPKPVAAELYNVSPRDP
                     LYRQKIVEWSKANRGELEGVYTGPANPKVRDHIYKIWMDVLKHYPVDGLHFDYVRFAS
                     PDFDYSRTSLEKFHKWLKPQLRSEERKQLKEALRTNPLAAPEMFASKFADFQRAQVTM
                     LVERIYRAVKKRKPEALVSAAVFANDENAYTRRFQDWRRWLQMGILDVACPMAYSTDT
                     AVFQKQIEVATTTAHNAQRQVWAGIGAYRIPSESTVEKINVARALNAEGFILFSYDFT
                     ARPSELNPDGAYLERVRRAVFY"
     gene            2193..>2675
                     /locus_tag="VFV61_06315"
     CDS             2193..>2675
                     /locus_tag="VFV61_06315"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEX4900129.1"
                     /translation="MKRQLNTIISLLTLLFALSGAALGQEVTGALTGTVKDANGAAVK
                     AAAVTVTDSAKKLVVRTTQTDDDGAFTVSELHVGVYDVTVEAPNFKKHLESNVKVDVG
                     QRRAVMVTLEVGNVEEVVTVEANPVAVELSTPTVSTLINGEQARELSLNNRNWVQLIT
                     L"
BASE COUNT          602 a          758 c          730 g          585 t
ORIGIN      
        1 aacggtctcc agctcgagtg gaccaagcgc ttgagtcaca accttacctt ccaggcggca
       61 tacactttca gcaaagccat cgacacgact tccgaagcca cctttgtcgg tgcgggtgat
      121 tcgaatcaaa ccggtaacga tgccaggtcg gcacgcgccc tgtcgcggtt ccacacgccc
      181 caccggttta ctttcttcgg aacgtaccga acgccctggt tcagtaacga tcgcggcttg
      241 cttggacagg tacttggcgg atggcagttc tcgacggttg tgaaactcgc caaaggcact
      301 ccgtttacag tagtcaccac tggcgtcgat ctgaacctcg acgggttcgc agagtcgcgt
      361 cctattcttc tgaatccttc gatactggga cgtagcgtcg cagacccggc tactgcaact
      421 attagcctgc cgagttccgc gtttcgccag ctaacaatag ctgacagggg cgcttcgatt
      481 ctgggtcgca acactttctt cggcgacggt gtaaggaaca tcgacttcgg gatcttcaag
      541 aacttcccaa tgccgtggga aggtcaccgg ctgactctgc gagccgacat gttcaatgcg
      601 ttcaatcacg tgcagtacgg atttccgacg acagacataa ccaacgccaa ctttggccgt
      661 attctcggat tggggacgtt gtattcgcca cgcacgattc agttctcgtt acgttaccag
      721 tactgacatc atgcagatcc tgtaaatcat gtcatcttgt ctaaaactgt tcttgcgatt
      781 ctcagcaatc acattgcttt tgtctctgtg ctcccccttg ctgaacgcac agtcaaaaag
      841 cgacgaggtg cgcgcgctgt gggtcgtacg cacgacgctc acctcacccg agaagatcca
      901 tcaacttgtt tcatccgcgg ctgacaacgg cttcaacacg ttgattgttc aaattcgtgg
      961 tcgcggtgac gcgtactaca actcgcgtgt cgagccacga gcgatcgagt tgagagatca
     1021 gcccgcttca ttcgatccgc tggcactcac cgtcgcggaa gcacacaaac gcggactcaa
     1081 agttcacggc tggataaaca caaacctgct cgcaaatctc gatgccttgc cggtccaacc
     1141 ggatcatgtc tacaacaagc atccagagtg gctggcggtg ccgaagccgg tggccgcgga
     1201 gctctacaac gtgtcgccac gcgatccgct ttatcgccag aagatcgtcg aatggtcgaa
     1261 agcgaatcgc ggcgagcttg aaggtgttta caccggaccc gcgaatccaa aagtgcgcga
     1321 tcacatctac aagatctgga tggacgtgct gaagcactat ccggtagacg gtttgcactt
     1381 tgactacgtc cgttttgcga gtcctgactt cgattacagt cgcacctcgc tcgagaagtt
     1441 tcacaagtgg ctgaagccgc agttgcgctc agaggagcgc aaacaattga aggaggcgtt
     1501 gcggacaaac ccgctcgctg cgccggaaat gtttgcgtcg aagtttgctg acttccagcg
     1561 cgcgcaggtc acgatgctgg tcgagcggat ctatcgagca gtcaagaaac gaaagccgga
     1621 agcgttagtc tcagcagccg tgtttgcgaa cgatgagaat gcgtacacgc gacgctttca
     1681 ggattggcgg cgctggttgc agatgggaat tctcgacgtg gcgtgtccga tggcgtattc
     1741 gactgacacc gcggtctttc aaaaacagat cgaggtcgcg acgacgaccg cacacaacgc
     1801 gcagcgccag gtctgggcag gaattggcgc gtaccgcata ccttctgagt ctacggttga
     1861 gaagatcaac gtcgcccgcg cgctcaatgc ggagggattc attctgttct cgtacgattt
     1921 cacggcgcga ccgagcgagt tgaatccaga cggcgcctat ctcgagcgcg tccggcgggc
     1981 agtcttttat tagacaggat gacaggattt gcaggatatg tcagaatatg cggactcgtt
     2041 tacggagcac gacctttcca tcaacaaccg tttgtctgac acgcgaaaat acaggtgtat
     2101 tgtggttctc ccagcgcctc gtcggcccat gcgttgctgc gcccggatcc gccggcaggc
     2161 ctcttccact aacccatgaa aaggactacc ccatgaaacg tcaactcaat accattatct
     2221 cgttgctgac actgctgttc gcgcttagtg gcgccgccct gggccaggaa gttaccggcg
     2281 cgctaacggg gactgttaag gatgcaaacg gcgctgccgt caaagccgca gccgttaccg
     2341 ttactgatag tgcaaagaaa cttgtagtgc gcaccaccca gactgatgac gacggcgctt
     2401 ttacagtcag tgagctgcac gtcggcgttt atgacgtcac cgtggaagcg ccgaacttca
     2461 agaaacatct cgaaagtaac gttaaggtcg acgtgggaca gcgacgcgcg gtgatggtga
     2521 ctcttgaggt gggtaatgtt gaggaggttg tcactgtcga agcgaacccc gtggcggtgg
     2581 aattgagcac gccaaccgtg tccacgctta tcaacggcga gcaggcccgc gagctatcac
     2641 tcaacaatcg caactgggtg cagctcatca ctctc
//
LOCUS       DASUEX010000378         2907 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_396364, whole genome shotgun sequence.
ACCESSION   DASUEX010000378 DASUEX010000000
VERSION     DASUEX010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2907
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396364"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..975
                     /locus_tag="VFV61_06320"
     CDS             <1..975
                     /locus_tag="VFV61_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900130.1"
                     /translation="PLLSNAAPRPAAPTERYVAPRNTSEPPPTEIYRPGAPFLNQVPE
                     MTPPRAAPPWPAHPQTYPAKKSNAVWWVVGILAIIGVFGVGVIVVILALASIDTNDNN
                     SSGNSNSVTLNDNRSTNNGSPYNNNFNASLSNSNINSTGPSNLSDDFASEKWRTGTFQ
                     FGEIWYSDDEFHMRAKEKMYLVMYAPNADYTTENATVRVTARSVDGVSPASGYGLIAH
                     GEKAADGSLEDYALLIYTGSDPQFQVVLHKNGVQKTLVPWTKSSVIRSGTNPNQLEIR
                     SQADELSFYINGRYLTRVTDTENNKRGVAGLYTSETGEVAFDDLEITR"
     gene            1001..2287
                     /locus_tag="VFV61_06325"
     CDS             1001..2287
                     /locus_tag="VFV61_06325"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing serine/threonine-protein
                     kinase"
                     /protein_id="HEX4900131.1"
                     /translation="MQVNLRVLAGPYKGRVFPFDEHDTFLIGRNNEAHLCLPDDRFFS
                     RNHCLLEIDPPRCFLRDLGSTNGTFVNGKRVSGTFLKHGDQVQGGATVLLIEVSTGWE
                     REETVDRRPSIVTVECLNCGRREQAQASTPDEKLTFLCEDCRAELKRSPQPIPGYEMV
                     RVLGRGGMGCVTLGREQKSGRAVAIKTLLPEAAVSDKAMRRFLREIEVAATLKHPNIV
                     EFLDRGTHNGVVYLITEFVDGPDAAKLADAHGGRLPYSLVVTIISQILDALAHAHGLG
                     FIHRDIKDQNILVRDALTNPQAKLTDFGLAKNFAQSGMSGVTMAGEAAGTIAYMAPEQ
                     LRNFRDVRPQSDIYGVGMTAYSLLTGEMALDLGGRTSVAEIVKAIFEQPLIPLERRAP
                     ELPHALCEVINRALHKDQTQRWQSAAAMRTALLHAV"
     gene            2584..>2907
                     /locus_tag="VFV61_06330"
     CDS             2584..>2907
                     /locus_tag="VFV61_06330"
                     /inference="COORDINATES: protein motif:HMM:NF014755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEX4900132.1"
                     /translation="MRSIWKGHIRFLLVAIPVRIYNAIETSEKIQFNQLHRDDYGPIG
                     YDKRCKKCNQVVTNDQITKGYQYEPERYAIIEPEDVAKVKIKTTKAVDIIGFVDAAEI
                     PTTFYD"
BASE COUNT          687 a          839 c          794 g          587 t
ORIGIN      
        1 ccgctgctca gtaacgccgc accgcgtcct gccgctccca ccgagcggta tgtcgcgcca
       61 cgcaacacga gtgaaccgcc gccgacagaa atctaccgtc cgggcgcccc attcctgaat
      121 caggtacccg aaatgacgcc gccgcgtgcc gccccaccgt ggcccgcgca tccgcaaact
      181 tacccggcaa agaaatcgaa tgccgtctgg tgggtcgtcg gaatactcgc aatcatcgga
      241 gtcttcggag tcggcgtgat cgtagtcatc ctggcactgg ccagtatcga tacgaacgac
      301 aacaactcga gcggcaactc caactcggtg actcttaatg acaatcgcag cacaaacaat
      361 ggcagtcctt ataacaacaa cttcaatgca agtctgagta actccaatat caactccact
      421 ggaccttcaa acttgagcga cgattttgca tccgaaaaat ggcgcaccgg aacatttcaa
      481 tttggagaga tttggtactc cgatgacgaa ttccacatgc gtgcgaaaga gaagatgtat
      541 ctcgtgatgt acgcgccgaa tgccgactac acgaccgaga acgccacggt ccgggtcacc
      601 gcgcgcagtg tagacggcgt ttcgccggcg tcaggctatg gcttgatagc gcacggggaa
      661 aaagcggcgg acggctcact tgaggactat gcgctgttga tctatacggg cagcgaccct
      721 cagttccagg tagtactcca taagaacggc gtgcaaaaga cactggtgcc gtggacaaag
      781 tcgtctgtca ttcgctcggg aaccaacccc aaccagctgg agattcgttc gcaagcggac
      841 gaactctcct tttacatcaa tgggcgctac ctcacgcgcg tcaccgatac cgaaaacaac
      901 aaacgcggcg ttgccggtct ctacacaagc gagaccggtg aagttgcttt tgacgatttg
      961 gaaatcacca gatagcccgc gggccggcgc agctaacgca atgcaagtca atctccgagt
     1021 cttggcggga ccttataaag gccgcgtctt ccctttcgac gagcacgaca cgtttctaat
     1081 tggccgcaac aacgaagctc acctctgcct gccggacgat cgcttcttct cccgcaatca
     1141 ttgtctcctg gagattgatc caccccgctg ctttctgcgt gatctcggct caaccaacgg
     1201 cacctttgtt aatggcaaac gagtcagcgg gaccttcctg aagcatggcg atcaagtgca
     1261 gggtggagcg accgtgttac tgatcgaggt cagcaccgga tgggaacgag aggagactgt
     1321 tgatcgccgg ccgtcaattg tgacggttga gtgcctcaat tgcggacgaa gagaacaggc
     1381 tcaggcctca acccctgatg agaaattgac ttttctctgt gaagactgtc gcgccgagct
     1441 gaagcgttca cctcagccga tacctggcta cgagatggtg cgcgtgctgg gtcgcggcgg
     1501 aatgggttgc gtaaccctgg ggcgagaaca gaaatccggt cgcgccgtgg caatcaagac
     1561 cctactcccc gaggccgccg tgagtgataa ggccatgcgc cgcttcctgc gtgagattga
     1621 agtcgctgcg accttgaaac accccaacat cgtcgagttt ctcgatcgcg gcacgcacaa
     1681 cggtgtggtt tacttgatca cggagtttgt tgacggaccc gacgccgcca aactagccga
     1741 cgcgcatggc ggtcgcctgc cctactctct ggtcgtaacc atcatctcgc agatcctcga
     1801 tgctctcgcc cacgctcatg ggctgggatt cattcaccgg gatatcaagg atcaaaacat
     1861 tttggtccgc gacgccttaa ccaatccgca ggccaagctc acggactttg gtctcgccaa
     1921 gaattttgcg cagtcaggta tgagtggagt gacgatggcg ggtgaagcag cgggtacgat
     1981 tgcttacatg gcgcccgagc aactacgaaa ttttcgcgat gtgcgaccgc agtcagacat
     2041 ctatggagtg ggcatgactg cgtacagctt gctcacgggc gaaatggcgc tggatctcgg
     2101 cggcaggaca agcgtcgcgg agatcgtgaa agcaatattt gagcagccgt tgattccact
     2161 ggaacgtcgc gccccggaat tgccccacgc gctctgcgag gtcattaatc gcgcgctgca
     2221 taaggatcag acgcaacgct ggcagtcagc ggcagcaatg cggactgcgc tgctgcatgc
     2281 ggtctgagct cgctaccccc tcctcgccag cgggtggggg ccgttcgggc caatcagcaa
     2341 tcacaagggc ccggctgtgc gtgcggcggg accaatctca tttcgagacc ccatctcaga
     2401 ttgccgcagc aacctcccct ttgagccaaa ggctccaaaa agagcaaaaa gccctgaaaa
     2461 ataaggcttt acttattttg tgaacgggcc gcataatacg tggcccctcg cctaccccag
     2521 agcggcgaga gctttcgcgg cgggatgtta tccttgagcc gctgttccgg gagagatgca
     2581 aaaatgagat cgatttggaa aggtcatatc agattcctgc tggtcgctat tccagttcgt
     2641 atttacaacg ccattgaaac gtcggagaaa attcagttta accagctcca tcgcgacgat
     2701 tacggcccca ttggctacga caagcgctgc aaaaagtgca atcaggtggt gacgaacgat
     2761 cagatcacca agggctatca atatgagccg gagcgctacg cgattatcga acccgaagac
     2821 gttgccaagg tgaaaatcaa gacgactaaa gcggtcgata tcattggctt tgtcgacgct
     2881 gcagagattc ctacgacgtt ttacgac
//
LOCUS       DASUEX010000379         4157 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_637846, whole genome shotgun sequence.
ACCESSION   DASUEX010000379 DASUEX010000000
VERSION     DASUEX010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4157
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_637846"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1862)
                     /locus_tag="VFV61_06335"
     CDS             complement(<1..1862)
                     /locus_tag="VFV61_06335"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEX4900133.1"
                     /translation="MKTYSTEAIRNLAVIGHGDAGKTQLVSSLLYVAGETPRWGKVDE
                     GTTVTDHEEDSIARKITLNIGLAHLDHQDTKLNFIDTPGYAAFVTHARPALRVADCAV
                     VVVDGVKGVEVQTEKTWAYANEFIVPRLMVITKLDKEHADFGAALESAQKTFSRAIIP
                     FTLPIGKEKDFRGVVDVVHMKAYELGADGQAKEIEIPSEGRDVVEKTRERLIELVAES
                     DDALMEKYFEQGTLEESDILPNISRAIARSKLCPVYAASAVTLVGLRALLKGIVEFAP
                     DPAHHEAEYGRPVDGGKDAEQLARRYSDSEPFSAYCFRTVADPFAGRINVMKVISGKL
                     QSDANVLNSSHGTMERLGALHVIQGKQLDKISEAHAGDIIACVKLKETRTGDTLCDKQ
                     APIVYDPVEYPEAAIAFAIEPKSRQDEEKISVALHKILEEDPALHFSRDSQTKEFLLS
                     GSGQLHVETVVEKLKKRYSVEVTLHPPKVPYKETITQRAEVQGRHKKQTGGRGQFGDC
                     KVIFEPLPRGGGFVFEDKIFGGAVPQQFRPAIEKGIVEAAMSGAIAGYPLVDFKVQLI
                     DGSYHDVDSDEHSFRAAGRKAFRAAMEKVKPSILEPIMDVEIAAPQENSGDIMG"
     gene            2164..2892
                     /locus_tag="VFV61_06340"
     CDS             2164..2892
                     /locus_tag="VFV61_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900134.1"
                     /translation="MLYKKLVTVFVVAIVTVAAAVASFAATSNHSDKTKGQRRVLTPV
                     NLAILIQDDLVSQVGNELDETRDFIRSLPAGSQVMVGYITTGTLQVRQPFTTDLEKAA
                     SSLRIVTSSTSASAFNPYVEVVEALRKFESGKGKNVLLLISDGLDTSRGFDSSAAGNT
                     LDLHRAIDNARKRDVAVYTFFAPSVGLTSHNRLAVSYGQSSLNRLANETGGKAFFQGS
                     SFVSFSPYFRRLREALNEQYARAY"
     gene            3369..3746
                     /locus_tag="VFV61_06345"
     CDS             3369..3746
                     /locus_tag="VFV61_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900135.1"
                     /translation="MREYCLIVEGAYLSESEAEHALRDPFLEDWVEQTGHFKIHNMDE
                     IQIAPGVTLGSLGVVMLDDRVFEIASVDGEHPLTEHKAKGVAEALRRQDMFEEIKVEL
                     RGEDSEGISLGNHETVGRDETSL"
     gene            3730..>4157
                     /locus_tag="VFV61_06350"
     CDS             3730..>4157
                     /locus_tag="VFV61_06350"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: protein motif:HMM:NF001664.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HEX4900136.1"
                     /translation="MKPAFEFLDGRLVVIIGDITQQQVEAIVNAANSSLLGGGGVDGA
                     IHRVGGPEILAECRDIRRTSHPQGLPTGEAVITTAGRLPAKHVIHTVGPIYGSHNGTE
                     AALLAACYENSLTLAASHSLSSVAFPSISTGAYGYPKPEAA"
BASE COUNT          906 a         1120 c         1123 g         1008 t
ORIGIN      
        1 tcgcccatga tgtcgccgct gttttcctgt ggcgccgcga tctccacatc catgattggt
       61 tcaaggatcg agggcttcac tttttccatc gctgcccgaa aggctttccg gccggcagcg
      121 cgaaacgaat gttcgtcgga atcgacatcg tgataggagc catcgatcaa ttgcactttg
      181 aaatccacga gcggataacc ggcgatggct cccgacatcg ccgcttccac aattcctttc
      241 tcaatcgccg ggcggaactg ttgcggcacg gcgccgccga agatcttgtc ttcgaaaacg
      301 aaaccgccac ctcgtggtag tggttcaaag ataaccttac agtcaccaaa ctgcccgcgc
      361 ccgccggtct gttttttgtg ccggccctgt acttccgcac gctgagtgat cgtttctttg
      421 taaggaactt tcggcgggtg caacgttact tctacgctgt agcgcttctt cagcttctcg
      481 acgaccgttt ccacgtgaag ttgtccggaa cctgataaca agaactcctt ggtctgcgaa
      541 tcccggctga aatggagcgc cggatcttct tcgaggatct tgtgcaatgc gacgctgatc
      601 ttctcttcgt cctgacgtga cttcggctcg atcgcaaacg cgatagcggc ttcggggtat
      661 tccaccgggt cgtaaacaat cggcgcctgc ttgtcgcaga gcgtatcgcc ggtgcgcgtc
      721 tctttcagtt ttacgcaagc gatgatgtca cccgcatggg cttcgctgat cttgtcgagc
      781 tgctttcctt gaattacatg gagagcgccc aggcgctcca tcgttccgtg gctggagttc
      841 aagacgttgg catcgctttg taacttgccg ctgatcactt tcatgacgtt gatgcgtccg
      901 gcaaagggat cggcaaccgt gcggaaacag taagctgaga agggctcgct atcactgtaa
      961 cgtcgcgcta actgctcagc gtctttgccg ccatctaccg ggcggccata ttccgcttcg
     1021 tgatgggcgg gatcgggcgc gaactcgacg atacccttga gcaaggctct cagtcccacc
     1081 agggttaccg cggatgcagc gtacacagga caaagtttgc tgcgcgcgat tgccctacta
     1141 atgttgggca ggatgtccga ctcttcgagc gtgccctgct caaaatactt ctccatcaac
     1201 gcgtcgtcag attcagcgac caattcgatc aagcgttcgc gcgtcttttc aacgacgtcg
     1261 cggccctcgc tggggatctc gatctccttg gcttgtccgt ctgctccgag ttcgtaagcc
     1321 ttcatatgaa caacatcgac tacgccgcga aaatcttttt ctttcccgat cggtaacgtg
     1381 aacggaataa tcgctcggct gaatgtcttc tgggcgcttt ccagggcggc gccaaaatcg
     1441 gcatgttctt tatccagttt tgtaatcacc atcaaacggg gcacgataaa ttcattagcg
     1501 taggcccatg tcttttcggt ctgcacttca acgcccttga ccccatcaac aaccaccaca
     1561 gcgcagtcag cgacgcgcaa ggccggacga gcgtgggtta caaatgcggc gtatcccggc
     1621 gtgtcgataa aattgagttt tgtgtcctga tgatctaaat gggcaagtcc gatattgagg
     1681 gtaatctttc gggcgatgga atcttcttcg tggtcagtaa ccgtagtgcc ttcatcaacc
     1741 tttccccaac gaggtgtttc gccggcgacg tagagaagcg aacttaccag ttgcgtcttc
     1801 cctgcgtcgc cgtgtccaat gaccgcaagg ttgcggattg cttctgtcga ataggttttc
     1861 ataaaagatt cctttcaggt gtgcgaattt ggcgaaggac accggaaacc gcggacatct
     1921 gtgcccgcct caatcgttgc tacagaggca aaattcttgc ctgcactgtg cttccggatt
     1981 gcgagtaatt tatccgacgg agaaaggtaa acgcaagttg gctcagtatt ccgacccgcc
     2041 cccaccagtc attaacaacg gccatctctt ttagggcctc gggcatcgaa gtatccggaa
     2101 acagcaggga gatttccggt agcgaagtaa ccggaaatag agggagattc tgagaggagt
     2161 atcatgcttt acaagaagtt ggttacggtt tttgttgtcg ccattgtgac agtggcggcg
     2221 gcggtcgcat cttttgcagc gacatccaat cacagtgaca aaaccaaggg tcaacgacgt
     2281 gttttgacac ccgtgaatct ggcgattttg attcaggatg atctggtgtc ccaggtggga
     2341 aacgagcttg acgagactcg cgatttcatt cgttcgctgc ccgcaggttc ccaggttatg
     2401 gtcggttaca ttaccaccgg aacgctgcaa gtgagacagc catttactac ggatctggag
     2461 aaggccgcca gctcgctccg cattgtaacg tcgtccacga gcgcttcggc ttttaatccg
     2521 tacgtcgagg tagttgaggc gttgcggaaa tttgaatccg gcaagggtaa gaatgtcttg
     2581 ctgttgattt cggatggctt ggatacgtcc cgtggttttg attcaagcgc tgccggcaac
     2641 acactcgact tgcaccgggc gattgataat gctcgcaagc gcgatgttgc tgtctatacg
     2701 tttttcgctc cgtcggtggg tctgacgagc cacaatcggc tagccgtgag ctatggtcag
     2761 agttcgctga accggttggc aaacgaaacg ggcggcaaag cgttcttcca gggaagtagc
     2821 ttcgtgagtt tcagtccgta ttttcgccgg ctacgcgaag cattgaacga gcaatacgcc
     2881 agggcttatt gaaaataaac ctgctgaaca cctacgaacg ccgtcgggtt aactccggcg
     2941 gcgttttatt ttggtgattg agggcgtccc gcgcgcaatg tgatcggagg cgcggcgtct
     3001 cgccctcaat gaggtacgaa gcgcgaataa aacataagcc cagataggca tcttaatttg
     3061 aaatccgtta attgttttct gatccgtgca tctgcgggta tctgtggcca aaaacttctt
     3121 cgcgctgcgc cctcgtgcgg gcgagacgcc cgcgatccct gagcccaact gcttctggtc
     3181 ggaatccgtg cccccgaaca attggcaatt cgtaatcggc aatcggcaat cgccttggcc
     3241 tccggtaagc cgcgatggta aagttgcgca cgcaaccagt acctcgtgag aaaaacgcac
     3301 aagatgagtt attgaaattg agtccgggag tcactggtta tgactctcgt tttaattaga
     3361 ccggcaaagt gcgcgaatac tgtctaattg tagaaggggc ctacctatcg gaatctgaag
     3421 ccgaacacgc cctgcgagat ccattccttg aggattgggt cgaacagacg ggtcacttca
     3481 aaatccacaa catggatgag atacaaattg caccgggcgt tacgctcgga tcccttggtg
     3541 tcgtcatgct tgatgatcgc gtattcgaaa tcgcgagcgt cgacggcgaa catcccttaa
     3601 ccgaacacaa ggcaaaagga gtcgctgagg cattgcgccg ccaggacatg tttgaagaga
     3661 tcaaggttga gctacgaggt gaagactcag aaggtatttc gctcggcaac cacgaaactg
     3721 taggtcgaga tgaaaccagc ctttgagttt cttgacgggc gcctcgtcgt cattattgga
     3781 gacatcacgc aacagcaggt tgaggccata gtcaacgccg ctaattcgag cttgctagga
     3841 ggcggtggcg tcgatggcgc cattcaccgc gttgggggac cggagattct tgcggagtgc
     3901 cgcgatattc gtcgcaccag ccatccccag ggattgccaa ccggcgaggc ggtcattacc
     3961 accgccggaa ggttgccggc gaagcatgtg attcacaccg tcggtcctat ttatgggagt
     4021 cacaacggaa ctgaagccgc gttgctagcg gcctgttatg aaaactcact aacacttgcg
     4081 gccagccact ctttatcctc cgtagctttt ccctcaattt ccactggagc atatggctac
     4141 cccaaacctg aggcggc
//
LOCUS       DASUEX010000380         2819 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_637862, whole genome shotgun sequence.
ACCESSION   DASUEX010000380 DASUEX010000000
VERSION     DASUEX010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2819
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_637862"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            371..715
                     /locus_tag="VFV61_06355"
     CDS             371..715
                     /locus_tag="VFV61_06355"
                     /inference="COORDINATES: protein motif:HMM:NF036463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5335 family protein"
                     /protein_id="HEX4900137.1"
                     /translation="MKTRQIPRAEWTGFFDELSKKLEGKSVALEVFGPEIGDQVEERQ
                     MFFAGLTADVTDKAGKQDQIEIMLGGNPSAHLTHLITAPIEVDLQQTELGVASALQIK
                     AADGTTSLLRLS"
     gene            808..1473
                     /locus_tag="VFV61_06360"
     CDS             808..1473
                     /locus_tag="VFV61_06360"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEX4900138.1"
                     /translation="MFCFTLLVILFLLSAFTVAGLATELNLKPQALGAREILEAEQRL
                     AELGYWAGPIDGVFDSGSRHALVAFQKVEGRKRTGKLTSAELASLRLAVRPLPRAGSY
                     AHAEIDLQRQVLFLVDDSGAVTHILPVCTGNDKSYVDHGQVHRAHTPRGQFKVIRKIR
                     GTRISSLGLLYYPNYIHEGIAIHGSWTMPAYPDSHGCIRIPMFTAKKFSELLPLGTEV
                     IVY"
     gene            complement(1563..2288)
                     /locus_tag="VFV61_06365"
     CDS             complement(1563..2288)
                     /locus_tag="VFV61_06365"
                     /inference="COORDINATES: protein motif:HMM:NF014090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HEX4900139.1"
                     /translation="MKKMEHDHSAEAISERLAAGPSHNYLRDWIYGGIDGAVTTFAVV
                     GGVAGASLSPWIILALGFANLFADGFSMAASNFLGTRAEHDDWRRLEATEYRHIELAP
                     EGEREEVRQIFQMKGFAGDDLERIVDLVTANRERWVRTMLTEEYGLPWEVRSPWVAAI
                     STFTAFLTCGLIPLLPYLLKVSYAFEVSMIITGITFVAIGSAKSKWSTVSWWRSGLAT
                     LLVGAIAAALAYAAGMLLKDLLT"
     gene            2528..>2819
                     /locus_tag="VFV61_06370"
     CDS             2528..>2819
                     /locus_tag="VFV61_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900140.1"
                     /translation="MNRKVYLIGALVLVICSSLARGQDVTKPTKDDDKIVSATNLVTV
                     NVIVTDKRGNYVNGLRREQFSVIDEKVKQQIAHFSAEAAPVSIAIVCELHDSP"
BASE COUNT          692 a          767 c          749 g          611 t
ORIGIN      
        1 atacgccgca atcggcctcc cacattgttt tggccgcctc cgccaaactg gcgttcaagt
       61 cgcagacttg ggcctcacaa gtcatgatgt ctttgacttt cataaaagtc cctcctcaca
      121 gatttccctg ttaagcgtta agtaggcaac gagcatgccc gccaaacgtc tggagaagtc
      181 aattgttttg tgggcgtgcc ggcacttgct tggggaaagt tactcactat cctcgcgaaa
      241 actcgcagaa tttttgtctt cggacactca gcggctgcct ttaattcaac caaatcagct
      301 ggcatgaatc atgcagccga cagcttcagg ggttaggaac ctggcgggag acaaaatgat
      361 ggagaacaca atgaagacac gacaaatacc acgagcagaa tggacggggt ttttcgacga
      421 gctgagcaag aagttggaag gcaaaagcgt tgccctcgaa gtgttcggtc ccgagattgg
      481 cgaccaggtg gaagagcgcc agatgttctt tgcagggctt accgccgatg tcacagacaa
      541 agccggcaaa caagaccaga tagaaatcat gctgggcgga aatccgagcg cgcacttgac
      601 gcacttgatc acggctccga tcgaggtgga cctgcagcaa acagagctcg gcgtagcgtc
      661 ggccctgcaa atcaaagctg cagacggcac caccagcctc ctgcgcttga gttgagctaa
      721 ggagttaccg tccgaattgg aaagtgggat tgcccttcgg aacggtaaac cggaatgcca
      781 tctgctgagc aaaatctagg tcgcaggttg ttttgcttta cccttctggt aatcctgttt
      841 ctgctttcgg cattcaccgt tgccgggctt gcaacagagc taaacctaaa gccacaagca
      901 cttggcgcca gggaaatcct cgaagctgaa caacgacttg cagaattggg ttattgggcc
      961 ggaccgatcg atggggtttt tgattcggga tctcgacacg cgctggttgc ttttcagaaa
     1021 gtcgaaggcc gcaaaagaac cgggaagcta acaagtgcgg aacttgcttc tttgcggctg
     1081 gcagtgcggc cgcttccgcg agctggcagt tacgcacacg cagaaataga tctccagcgt
     1141 caggtgttgt ttctggtcga tgacagtgga gcagttaccc acatccttcc cgtttgcaca
     1201 ggcaacgaca aatcctacgt agaccacggc caggttcatc gagctcacac gccgcgggga
     1261 cagttcaagg taatcaggaa gattcgcggg actcgtatta gctctctcgg tctgctctat
     1321 tatccaaatt acatccacga aggaattgcc atacacggga gttggacaat gcctgcctac
     1381 cccgacagcc acggctgcat tcgtatcccc atgttcacgg cgaagaagtt cagtgagctt
     1441 ctgccgctgg gaacggaagt cattgtctac tagacagggc gattgccgat cggcgattgg
     1501 cgattggctt cctgtgttag agggtcaatt ggcaatcgcc aatcgcaaat gggcaatctc
     1561 tctcacgtaa gcagatcttt cagaagcatg ccggcggcgt aagcgagggc agcggcaatt
     1621 gcgcccacca gcaacgtcgc aaggcccgag cgccaccacg aaacagttga ccacttgctc
     1681 ttggcggatc caattgcgac gaaggtgatg ccagttatga tcatcgagac ttcgaacgcg
     1741 taagaaacct ttaacaaata cgggagcaga gggattaagc cacaagtcag aaaggcggtg
     1801 aaggtgctga tagccgctac ccagggcgaa cgcacttccc aggggagtcc atactcctct
     1861 gtcaacatcg tccgcaccca gcgctcacga ttggcggtta ccaggtcgac aatcctttcg
     1921 agatcgtctc ccgcaaaacc cttcatttga aaaatctgtc ttacttcctc gcgttctcct
     1981 tcaggcgcca gttcaatatg gcgatattcg gtcgcctcca gccggcgcca gtcgtcatgc
     2041 tccgccctgg taccaaggaa gttactggcg gccattgaaa agccgtccgc aaacaaattg
     2101 gcaaaaccca aagccagaat gatccaggga gaaaggcttg caccggctac accaccgaca
     2161 accgcgaagg tggtcacggc gccgtcgatc ccgccgtaga tccaatcgcg cagatagtta
     2221 tgcgatggtc cggcggccag tcgctcgctg attgcctccg ccgaatgatc gtgttccatt
     2281 ttcttcatcg tgcttcctac catcctcggc aatgatcatg ccgagataac cagacgcgtg
     2341 ctctgctgac caattcctgc ccacagttga gccgaggctg cgggcttttg cgcaagcttt
     2401 gggaaactta ctcagcagca gcccctgcgc cgcacctttt tcccctgaaa agccagagaa
     2461 ttgccgggca tattgcttgc tcaaacacat gacagacgtg acggttctgc gggctaaagg
     2521 agtggtcatg aacaggaagg tttacttgat cggggctctg gtcctggtca tttgcagttc
     2581 gctagcacga gggcaggacg ttactaaacc aactaaagac gacgacaaaa tcgtcagcgc
     2641 tacgaatctt gtgacggtga acgtaatcgt cacggacaag cgcggcaact atgtgaatgg
     2701 tctgagacgc gaacagtttt cagttatcga cgagaaagta aaacaacaaa tcgcacactt
     2761 ctctgctgag gcagcgccag tgtcgattgc gatcgtctgt gagcttcatg attcgcctt
//
LOCUS       DASUEX010000381         4341 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1444070, whole genome shotgun sequence.
ACCESSION   DASUEX010000381 DASUEX010000000
VERSION     DASUEX010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4341
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1444070"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..790
                     /locus_tag="VFV61_06375"
     CDS             <1..790
                     /locus_tag="VFV61_06375"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEX4900141.1"
                     /translation="NFAINVAREAGGVLVDKLGRALQVSNKGAIDLVTEADLAAERLI
                     IERIRSHYPRHAVLAEESGASEDNTGNSAGEWKWIIDPLDGTTNYAHGYPCFCVSIGV
                     ERAGKLELGVVYDPMRNEMFAAESGQGATLNERPMRVSGIEELSRAMLCTGFPYDVRE
                     RSDFARDFSNFTMHAQAVRRDGSAALDLAYVACGRFDGFWEDGLKPWDIAAGVLLIEE
                     AGGSVTDFQGAPLDIYSPRVLASNGLVHRAMIQVIQDRVAKELT"
     gene            complement(774..1340)
                     /locus_tag="VFV61_06380"
     CDS             complement(774..1340)
                     /locus_tag="VFV61_06380"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX4900142.1"
                     /translation="MGRGFWNLKLRGLENIPAAGGLLIAANHQSYLDPFLISVPIRRP
                     LRFLAWNEALEWPVAGKVMKLMGAWPLQLQGSDPAAIRRSIQWLRDAGALVIFPEGGR
                     GLPNGSMVRFKGGAARLALEAGVPILPVTIRGANRVWPAGKLVPRMGQIEVVYHPLHH
                     VIQHEGEETRECARRESDELAKIIKSAL"
     gene            complement(1478..1708)
                     /locus_tag="VFV61_06385"
     CDS             complement(1478..1708)
                     /locus_tag="VFV61_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900143.1"
                     /translation="MATSKPLIIPREVLLVEIDRRCSFADCDNRVGIGLTKQEAADYL
                     GFECEHCKRWNDDKLSQRDIPDWWSEVHTFDQ"
     gene            complement(1715..2452)
                     /locus_tag="VFV61_06390"
     CDS             complement(1715..2452)
                     /locus_tag="VFV61_06390"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX4900144.1"
                     /translation="MTKERPWKRIRERLELKLPVRVQCRETSDFDWVEITRLTNVTPF
                     GAGFTLKRPIERGRLVHMTIPMPRQLRVFDHLEDQYRVWALVRYLRSRSDTQEPATVF
                     DVGVAFVGKRAPKSYEADPTRRYEIAVSATEKRNLTEDPDKVVGPLSGTDKRAFTRHN
                     IAVDMKLETLNLKGEVDATEDTVTENISRQGATIFTGQVIPPGRFIRLTSQQFQLTVY
                     AAVRGQTTGADGMPRVHVEFVDQEWPL"
     gene            2816..>4341
                     /locus_tag="VFV61_06395"
     CDS             2816..>4341
                     /locus_tag="VFV61_06395"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing)"
                     /protein_id="HEX4900145.1"
                     /translation="METTERNHINIEDEMRRSYLDYAMSVIIGRALPDVRDGLKPVHR
                     RVLWAMHELGNTFNKAHKKSARVVGDVIGKYHPHGDTAVYDTIVRMAQDFSMRYPLID
                     GQGNFGSVDGDAAAAMRYTEVRMSKITGEVLSDIEKETVDFQPNYDESLSEPKVVPTR
                     IPNLLINGSDGIAVGMATKIPPHNLKEIVDATIALLSDPEIAINALTKIVTGPDFPTG
                     GFIYGREEIRKSYTEGRGILQLRARAGIDRIGRGAQERDAVVITEIPFQVNKARLIER
                     IAELSNEKKLEGISDLRDESDRSGMRIVVELKRDAVPQVVLNKLYKLTPMQTSFGVIN
                     LAIVNGQPRVLNLKQMLESFIEFRREVVRRRTEYELRKARARAHILEGLTKAIDALDF
                     IVTLIRNSRSVDEARLWLTAQMATMSEVKVWKGAPTDVALKTYLSKMQKTMERLGFSE
                     IQAQAILDLQLRRLSALERQKITDEYEAIIKHIAELENILSNENALRRVIKSELEDVK
                     "
BASE COUNT          999 a         1166 c         1192 g          984 t
ORIGIN      
        1 caacttcgca attaacgtgg cccgtgaggc cggaggcgtt ctcgtcgata aactgggccg
       61 ggcgcttcag gtttccaaca aaggtgctat cgacctcgtc accgaggcag atcttgctgc
      121 tgagaggtta ataatcgaac gcattcgttc acactatccg cgccacgccg tcctggctga
      181 ggaatcaggc gcttcagaag acaataccgg aaactctgcg ggtgaatgga aatggatcat
      241 cgatccactc gacggtacga ctaactacgc gcacggttac ccttgctttt gtgtttcgat
      301 tggagtggag cgagcaggca agttggaact gggcgtggtc tacgatccaa tgcgtaatga
      361 gatgtttgcg gccgaaagtg gtcagggagc gacgctaaac gagcgaccca tgcgcgtctc
      421 aggtatagaa gagctaagtc gcgcaatgct ttgcaccggc tttccttatg acgtgcggga
      481 acggtctgac tttgctcgcg atttttctaa cttcacaatg cacgctcagg cagtgcgtcg
      541 cgacggatcg gcagcgttgg acttagcgta cgtcgcctgt ggacgctttg atggattctg
      601 ggaggacggc ttgaagccct gggatattgc tgccggagtc cttttgattg aagaagccgg
      661 tggcagcgtt acggactttc agggagcgcc actcgatatc tactctcccc gcgtgctcgc
      721 cagcaatggc ctggtgcacc gcgcgatgat acaggtaatt caagatcgag tggctaaaga
      781 gctgacttga ttattttggc gagctcatcg ctctcgcgcc gcgcacactc gcgagtctct
      841 tcaccttcat gctggataac atgatgcaat ggatggtaaa caacttcgat ttgtcccatc
      901 cgcggcacca gctttcctgc cggccaaacg cgatttgcac cgcgaatagt tactggaaga
      961 atcggtacgc ccgcttccag tgcgagccgg gccgcgccgc ctttgaaacg caccatcgag
     1021 ccgtttggta atccgcgacc tccttcggga aagatcacga gcgcgcccgc atcgcgcagc
     1081 cattgaattg agcgacgaat tgctgccgga tcgcttcctt gcaactgcaa tggccaggct
     1141 cccatcagct tcatcacttt ccccgcgacc ggccattcga gtgcctcgtt ccaggcgaga
     1201 aagcgcaagg ggcgacggat cggaaccgag atcagaaacg gatcgaggta tgactggtgg
     1261 ttcgccgcaa tcaacaaccc acccgcggcg ggaatgtttt ccagacctct tagcttcaga
     1321 ttccagaatc cacgacccat caggccaaag actggtcgga tcaaatcaag cgtggcttga
     1381 ggtagaaccg gagactctac ctttgatgga cgggtagttg tttggctcga tctcacgtag
     1441 gttcaatatt gccggatcaa gtgattcaac aagtagctta ttgatcgaag gtgtgaactt
     1501 cggaccacca atcgggaatg tctctttgcg atagcttatc gtcgttccaa cgcttgcagt
     1561 gttcgcattc gaacccgaga taatccgcgg cttcctgttt tgtcaaacca attccgaccc
     1621 ggttatcaca gtcagcaaat gagcaacgcc ggtcaatttc gaccagcaaa acttcgcggg
     1681 gaatgatgag aggttttgaa gtcgccatcg gaatttagag gggccactct tggtcgacga
     1741 attctacgtg aacgcgcggc attccatcag cgccggtagt ttggccccgc accgcggcgt
     1801 aaacagtcaa ttgaaattgc tgactggtta gacgaatgaa ccgccccggg ggaattacct
     1861 gaccggtaaa aatggtggca ccctggcgac tgatattctc cgtcacggta tcttccgtcg
     1921 catcaacttc acccttcagg ttcagggttt ctagcttcat gtctacggcg atgttgtgcc
     1981 gagtgaaagc acgcttgtcg gtacctgata gcggaccgac gaccttgtca ggatcttctg
     2041 tcaaatttcg cttctcagta gccgataccg caatttcgta acgcctggta gggtcagcct
     2101 cataactctt cggggcccgc ttacctacga aggcgacacc aacgtcaaat acggtcgctg
     2161 gttcctgagt gtctgaccgg gatcgcaggt accgcaccag cgcccacacg cgatactgat
     2221 cttcgaggtg gtcaaagacg cggagctggc gcggcatcgg gattgtcatg tgcaccaggc
     2281 ggccccgctc gatgggtcgc ttcaacgtaa atcccgcgcc gaacggagtt acatttgtca
     2341 gtcgcgtgat ctcaacccaa tcaaaatccg aagtttcgcg gcactgcaca cggacgggca
     2401 gctttagctc aagacgctcg cggattcgct tccagggtcg ctctttagtc attgtaaatt
     2461 tttgcggtta tgcaacgccg gcagcaacgc cgcaacctag ctttgttcgg tactcagaat
     2521 cgctcaaact gggttcacct ttccgcttag aagctgttga ttttattagc ctttatcctc
     2581 acttccgggt ttgccaaacc tacggcaaga ggctacaata gcctgccatt taagtccatg
     2641 gactaccagt ctttgcgagc agcaaacacc cttgaatccg gtaatgaact gagctttgtg
     2701 aagggcttca ttttggggga gtcgcgagtg gcgctttgtc ccgaggtaag tctggtggcc
     2761 ctgagaatga catcgaaacg ttcgaccggc ggattgtact ggaaaggtta ttgagatgga
     2821 aactactgaa cgcaatcata tcaatatcga agacgagatg cggcgttcgt atcttgatta
     2881 tgcgatgtcg gtcattatcg gccgcgcgct tccagatgtg cgcgacggat taaagccggt
     2941 tcatcgtcgt gtgctctggg ccatgcacga gctcggcaac actttcaaca aggctcacaa
     3001 gaaaagcgca cgcgtcgtgg gcgatgtgat tggtaagtac catccgcacg gcgacactgc
     3061 cgtttacgac accatcgtcc gcatggcgca ggatttctcg atgcgctacc ccttgattga
     3121 cggccaggga aattttggat cagttgacgg tgatgccgcg gcggccatgc gctacaccga
     3181 agtgcgcatg tcgaagatca ctggagaggt gttgtcggac attgagaaag agacggttga
     3241 ttttcagcct aactacgacg agtcgctgag cgaacctaag gtggtaccca cccgtatccc
     3301 caacctgcta atcaatggtt cggatggaat cgcggttggc atggctacca agataccgcc
     3361 tcacaacctc aaggagatcg tggacgcgac gattgccctg ctttccgatc ccgagatagc
     3421 gattaacgcc cttaccaaga tcgtcaccgg tccggacttt cccacgggtg ggttcatcta
     3481 cgggcgcgag gaaatcagga agtcgtatac ggaaggccga ggcattctgc aattgcgcgc
     3541 ccgtgcgggt atcgaccgca tcggtcgtgg tgcacaggaa cgcgatgcgg tcgtcattac
     3601 cgaaataccg ttccaggtta acaaggcccg gctcatcgag cgtatcgccg aactcagtaa
     3661 tgaaaagaag ttggaaggca tttctgacct gcgcgacgag tctgaccgca gtgggatgcg
     3721 tatcgtggtt gaactgaaac gcgatgcggt accgcaagtt gtgctcaata agctctacaa
     3781 gctcacaccg atgcaaacct ccttcggcgt gatcaacctg gcaattgtta atggtcagcc
     3841 gcgagttctg aacctgaaac aaatgctcga gagctttatc gagtttcgcc gcgaagtagt
     3901 gcgacggcgg accgagtacg agttgcggaa agctcgcgca cgcgcccata tcctcgaagg
     3961 actcaccaaa gcaatcgatg cccttgattt cattgtcact ttgattcgca actcgcgctc
     4021 agttgatgaa gcgcgcctgt ggctgaccgc ccagatggcc acgatgtctg aggtaaaggt
     4081 ctggaaaggt gccccgactg acgtagccct gaaaacctac cttagcaaga tgcagaagac
     4141 gatggagcgg ctgggattct ctgagatcca ggcgcaagcg attctcgact tgcaactgcg
     4201 ccgtttgtcc gcgctcgaac ggcagaaaat taccgacgag tatgaggcga taatcaaaca
     4261 catcgcggag ctggaaaata tcctttccaa tgagaatgct ctgcgccgcg taatcaaatc
     4321 tgaacttgaa gacgtgaaga a
//
LOCUS       DASUEX010000382         2218 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1108025, whole genome shotgun sequence.
ACCESSION   DASUEX010000382 DASUEX010000000
VERSION     DASUEX010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1108025"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1021
                     /locus_tag="VFV61_06400"
     CDS             <1..1021
                     /locus_tag="VFV61_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="cell wall biosynthesis glycosyltransferase"
                     /protein_id="HEX4900146.1"
                     /translation="YRGIGGKGTALRAIFEAADKLNARACAVVDADLRSITPDWVRYL
                     LEPVLDKNYDYVAPIYSRYKWDGTITNNVAYNLTRALYGKRIRQPIGGDFGLSSNTVR
                     SYLTHNVWMTDVARFGIDIWMTTEAIAGGYRVCQANLGVKVHDAKDPGMSLGPMFRQV
                     VRTLFTLMEQHAPIWKEIHGSEAVETFGASEFPEPEAIKVNQELLIQNFKLGFQHFGP
                     VWKEILSSACYATVQKLASSERKNFLLDVISWAHILYDTAATFHHWPKDRTTLVDLMS
                     PLYEARVASFINETAEMSTAEAERVVEQQAEIFETEKPYLLKLWDAGEKEPRAKGMLQ
                     KMFGG"
     gene            1067..>2218
                     /locus_tag="VFV61_06405"
     CDS             1067..>2218
                     /locus_tag="VFV61_06405"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX4900147.1"
                     /translation="MANTKILLPVVHAPSARADLELAVRLLHPQGKIIALNVVRVPEA
                     NSLSEGAGEAVDSRAALEELYTLLPDQRVELKTLVKVSRSVTEGIAESAAAEGADLLL
                     LPWKGSTNSEVQFFGRTIDKLVESPPCNLLVSRVGELSNCKHILLPVRGGPYTEFAMQ
                     IASQLAQSFAGEITVLHCEQPFVSPEFGQALYGAFIQRLRFQPQVKRVVTVRGDPKEA
                     IIQEAADQDLVIIGAGAMMEPQSFFLGPVVEQIAKATDKPLLVLKTPEPFAAWSRDRR
                     LIQPKTLSQRVDQWFAENTFHRREFEELDKLVELKRKQGVTISLGLPALNEEETVGSV
                     IRVIKQKLFDAVPLLDELVLIDSGSTDDTVKIAKDLGIPVYVHQEILPQH"
BASE COUNT          476 a          625 c          660 g          457 t
ORIGIN      
        1 ttaccgcggc atcggcggca agggcacggc gctgcgcgcg atcttcgagg cggcggacaa
       61 gctcaacgcg cgcgcttgcg cggtggtgga cgcggatctg cgcagtatca cgcccgactg
      121 ggtgcgttac ctgttggaac cggtcctgga caagaattat gactatgtcg cgccgatcta
      181 ttcccgctat aaatgggacg ggacgattac caacaacgtc gcatacaatt tgacccgcgc
      241 tctgtacggc aagcggatac gccagcccat cggcggggat tttggcttgt cgagtaacac
      301 cgttcgctcg tatctcacgc acaacgtttg gatgaccgac gtcgcgcgct tcggcatcga
      361 catctggatg acgacggagg ctatcgccgg agggtaccgg gtgtgccaag cgaatctcgg
      421 cgtgaaggtg cacgatgcta aagatcctgg tatgtccctc gggccgatgt ttcggcaagt
      481 cgtgcggacg ctttttacgc tgatggaaca acacgcgccg atttggaagg aaattcatgg
      541 cagtgaagcc gtggagacat tcggcgcctc cgagttcccg gaacccgagg cgatcaaggt
      601 caatcaagag cttttgatac aaaatttcaa attggggttt cagcacttcg gcccggtatg
      661 gaaagaaata cttagctccg cctgttacgc aaccgtgcaa aagttggcga gttcggaacg
      721 caagaacttt cttctggacg ttatcagttg ggctcacatt ctctacgata ccgccgcgac
      781 gtttcaccac tggcccaagg accgaaccac cttggtggat ctgatgagcc cgctctatga
      841 ggcccgggtc gcgtctttta tcaacgagac ggcggaaatg agcacggcgg aggcggaacg
      901 ggtcgtcgag caacaggcgg aaatcttcga aaccgagaaa ccttatctgc taaaactttg
      961 ggacgccgga gagaaagagc cgcgcgcaaa gggaatgctg caaaagatgt tcgggggcta
     1021 gcgcgcgacc aaggattttg ccatcgctcg gagatttaat tccatcatgg ccaacaccaa
     1081 gatcttgctg ccggtcgtcc atgcgccttc agcgcgcgcc gacctggagc tggccgtgcg
     1141 cctgctccac ccgcagggaa agatcattgc gctgaacgtt gttcgagtgc cggaagcaaa
     1201 ctcgctgagc gaaggcgccg gggaagccgt cgattcgcgc gccgccctgg aggagcttta
     1261 taccctgctc ccggaccaac gcgtagagct caaaacactg gtgaaggtgt cccgtagcgt
     1321 gactgaaggt atcgcggaaa gcgcggcggc agagggcgcg gatttgcttc tgctgccctg
     1381 gaaaggaagc acgaattcgg aagtccagtt tttcggtcgc accatcgaca aactcgtgga
     1441 atcgcctccg tgtaatctgt tggtgtcccg agttggcgaa ttatccaatt gcaaacatat
     1501 cctccttccc gtgcgcggcg gcccctatac ggagttcgcc atgcagatcg cctcccaact
     1561 tgctcagtcg tttgcgggag agataacggt tcttcactgc gagcaacctt ttgtctcgcc
     1621 cgagttcggc caagcgcttt atggcgcctt cattcagcgt ctgcggtttc agccgcaggt
     1681 caagcgggtc gtcacggtgc ggggcgatcc caaggaagcg attatccagg aggcggccga
     1741 tcaagatctc gtgatcatcg gtgcgggagc gatgatggag ccccagtcgt tctttctcgg
     1801 tccggtcgtc gagcagatcg cgaaggccac ggataaaccg cttttggtcc tgaagacccc
     1861 cgagcctttt gccgcgtgga gccgcgatcg gcgcctgatt cagcccaaaa cattatccca
     1921 gcgcgtcgat cagtggttcg ccgaaaatac ctttcaccgc cgcgagttcg aggagctgga
     1981 caagctggtc gagctgaagc ggaaacaggg ggtcaccatc agtttggggt tgcccgcgct
     2041 caatgaagag gaaaccgtcg gtagcgtcat tcgtgttatc aagcaaaaac tcttcgacgc
     2101 cgtacccctg ctggacgagc tcgtgctcat cgactcgggt tcgacggatg acaccgtcaa
     2161 aatcgccaaa gatttgggca tccccgttta tgtccaccag gagatcctgc cgcagcac
//
LOCUS       DASUEX010000383         5318 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1199520, whole genome shotgun sequence.
ACCESSION   DASUEX010000383 DASUEX010000000
VERSION     DASUEX010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5318
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1199520"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(185..484)
                     /locus_tag="VFV61_06410"
     CDS             complement(185..484)
                     /locus_tag="VFV61_06410"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HEX4900148.1"
                     /translation="MPNRSVRVVARIEALPHRVAEVRAILFGLVAPTRNEAGCTTYEL
                     WQNRAADTDFTFVEEWESEAALDAHAASQHLKDATARLEGLIKQAPDVRRYDLVR"
     gene            complement(506..1435)
                     /locus_tag="VFV61_06415"
     CDS             complement(506..1435)
                     /locus_tag="VFV61_06415"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEX4900149.1"
                     /translation="MLPEQDPNRPLVLLTGATGYVGARLLSLLEQQPLRLRCLARNPE
                     ALQTQVNAATEIVSGDVLDAPSLRRALDGVHTAYYLVHLMSSSADFQKDDRQAAINFG
                     LAAQKSGVRRIIYLGGLGDDRDPQLSPHLRSRHEVGQILRDSGVETIEFRASMVIGAG
                     SLSYELMKSLTERLPVMICPRWVSVRTQPIAIDDVLSYLLAAKDLAGTGSRIFEVGGS
                     DVVRYLDMIREYARQRGLHRWLISVPLLTPYLSGLWLALVTPASYEVGRHLIEGLKNP
                     TVVRDETAAEVFPIRPVGSRQAIAKALTKEHRS"
     gene            complement(1481..2071)
                     /locus_tag="VFV61_06420"
     CDS             complement(1481..2071)
                     /locus_tag="VFV61_06420"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX4900150.1"
                     /translation="MIKGSERRKPDHPVDEIFLNRWSPRAMTGEPIPQEDLLSLFEAA
                     RWAPSSYNNQPWRILYATRSGEHWQQFLELLVEGNQVWARNAGALLLFLSKKTFTYNG
                     KPSITHSFDTGAAWENLALQGSLKGYVVHGMQGFDYERARTTLSIPDDFQVEAMVAVG
                     LPGSPEVLPEQLRSKEAPSDRRKLEETICAGPFCFK"
     gene            2257..3096
                     /locus_tag="VFV61_06425"
     CDS             2257..3096
                     /locus_tag="VFV61_06425"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX4900151.1"
                     /translation="MPSHIMPNNCTSNAGLHCELYGSSGDPVLCLHGLGASTHSFREF
                     REPLSKEHKLFLLDFRGFGASPKPKDKHYAIMEHADSAYQFIVEHDLRNLTLIGNSFG
                     GAVSLLVSLRLCEENSGRLRRLILIDSGGYNLHLPTHLKMLRTPLLGSLMIHLLPPKL
                     AALTVLKDSYYDDRKITREQIAAYASPIAESGGRHALLETSRQIIPPNYEELIKKYPK
                     IVVPTLIIWGRQDKVIPLCIGKMLDAALPDSKLVVIDHSAHVPQEETPQAVVPLVLDF
                     LRS"
     gene            complement(3316..5007)
                     /locus_tag="VFV61_06430"
     CDS             complement(3316..5007)
                     /locus_tag="VFV61_06430"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEX4900152.1"
                     /translation="MKRLFIVVFVFVLISNSVIPVSAQQPKTPADLQKLAADYYNWRN
                     ENFPVASSDAGLHTWDNRLTDYSLSAVLARRLHAKEILAKVDAIPASGWSKDDRIDWM
                     LFRAQIDGVVFFNRVMDFEQSDPQVYVNECSNGIFSLLKKEYDTPRNRALAATERLRQ
                     MPQLLERGKQNLIKPVRLYAQLAIESARSIDSLFNESLMTLATGLNAAERNELVKARD
                     AALVALHGYADWLERRLPGMVAFKPMGEENYNYILKNIYLLPLDAKQVEMLGQAELAR
                     YRGLESQLSDPALADPNPARSKVIPADQQAFLKAYESRQEEMISFLKTNRLITIPDYI
                     GPFTIRQLPEAFKPTSPGGFMNPPGVYDKDNSGFYFIPTYNRDSKNFYIRAAIEDPRP
                     ILGHEGIPGHFLQLSIANHLSNEIRRQHSDGIFIEGWALYGEEMLLRSGLYPLNSAGN
                     GQVLRLSRYRAARIGVDVNLHTGKWTFEQAVNYFMEAGGLDREAATGEAAGAAASPTQ
                     KITYMVGKWQIMRLLGRYRDKQGANFRLGQFHDDLLKNGSLPLSIVEWILLDTQP"
BASE COUNT         1200 a         1455 c         1440 g         1223 t
ORIGIN      
        1 cggcatcgga ttcgcgatca cgcgctacga cctcatacaa gaaattcccg tcttcgtctt
       61 tgagtgcagc tagatgttca cgaaaaaggg agaagcggcc gacgtgccag ttgtcagggt
      121 cgaagggaat agtcgtctgc attaagatac gaatcggtcg tcccggcatg gcaatcctcc
      181 attgctagcg cacgaggtca taacgacgca cgtccggcgc ttgtttaatt aggccctcga
      241 gtctggcagt agcgtcctta agatgctggc ttgctgcgtg ggcgtcaagc gcagcttcgc
      301 tttcccactc ttccacgaaa gtgaaatctg tgtctgccgc tctgttttgc cacagttcat
      361 aagtagtaca accagcttcg ttgcgggtcg gcgcaactaa accgaacagg atcgctcgca
      421 cttcagcaac tctgtgcggg agcgcttcga tgcgcgcgac tactctgact gacctgttag
      481 gcatagctgt catcctctgt gagcctcaac tgcggtgctc ttttgttagc gccttcgcga
      541 tagcttgccg gctgcccact ggtcgaatgg gaaacacttc cgcagccgtc tcgtcacgaa
      601 cgacggtggg attcttcaga ccctcgatta aatggcgtcc cacctcataa cttgccggtg
      661 tcacaagtgc cagccagagg ccggagaggt agggcgttaa caagggcacc gagatcagcc
      721 agcgatgcaa gcctcgctgt ctggcgtatt cgcgaatcat atccagatat ctcactacat
      781 cagaaccacc tacttcgaag atgcgactac ctgtcccggc caaatctttc gcggcgagta
      841 gatacgacag cacgtcgtca atcgcgattg gttgcgtgcg aacactaacc cagcgcggac
      901 agatcatgac tggcagtctc tcggtcagcg acttcatcaa ttcgtaagaa aggctgccgg
      961 caccgatgac catcgaagcc cgaaactcga ttgtctccac gccggaatca cgcagaatct
     1021 ggcccacctc atgacgactg cgcaagtgag gcgagagttg cggatcgcga tcatcgccga
     1081 gacctcccag atagataatg cgtctaactc cggacttctg cgccgccagg ccaaagttga
     1141 ttgccgcctg ccggtcgtct ttttgaaagt cagccgaact ggacatcagg tgaactagat
     1201 aatatgcggt gtgaacccca tccagcgctc gtcggagtga tggcgcatcc aacacgtcgc
     1261 cagaaacaat ttcggtagcg gcattcacct gcgtctgaag cgcctctgga tttcgcgcca
     1321 gacaacgcag acgaagcggt tgctgctcaa gcaaagaaag cagccgggca ccgacgtacc
     1381 cggtggcccc tgtcaacaac actagtggtc ggtttggatc ttgttcagga agcatcaagc
     1441 tcttccgcgc ggttctgggg gctggtaaat gagaggggct ctatttgaaa caaaacggtc
     1501 cggcgcagat tgtctcttcg agtttgcggc gatcgctggg ggcttctttg gatcgcagtt
     1561 gctcgggtaa gacttcaggg ctaccaggga gaccaacggc caccattgcc tcaacctgaa
     1621 agtcgtcggg aatactcagg gtggtgcgcg cgcgttcgta gtcaaatccc tgcatgccat
     1681 gaacaacata gcccttgagc gagccctgca acgccaggtt ttcccaagcg gcgccggtgt
     1741 caaaggaatg ggtaatcgaa ggcttgccgt tgtaagtaaa cgtcttcttt gagaggaaga
     1801 gcagcaacgc gccggcattc ctggcccaga cttgattgcc ttcaacgagt agttctaaga
     1861 attgttgcca gtgctcaccg cttcgagtgg cgtagagaat gcgccatggt tgattgttgt
     1921 aagacgacgg cgcccagcgc gcggcttcga aaagtgataa cagatcttcc tgtggaattg
     1981 gctcgccggt catcgcacgt ggcgaccagc gatttagaaa tatctcgtca accggatgat
     2041 ccggcttacg gcgctcgctt cccttaatca tgcgatcctc cgctaattgt cgggggtatt
     2101 tgtttggtat tgggggttga aaaatatgat agcccgcgac gcatcttcca gcatagccgg
     2161 gttaatcgag aacaggaaaa cgctggcaac gctgcaactt gaattgccga ccgccttccg
     2221 gaccgagcct aactgacgcg tgccagatcg gctatcatgc cctctcacat aatgcctaat
     2281 aactgcacta gcaacgcagg acttcactgt gagctgtacg gcagcagcgg cgatccggtg
     2341 ctgtgtctcc acggtctcgg tgccagcact cactcgttca gggaatttag agaaccactc
     2401 tcgaaggaac acaagctgtt cctgctcgac ttcaggggct tcggagcctc accgaaacct
     2461 aaagacaaac actacgcaat catggagcat gccgattctg catatcagtt catcgtcgaa
     2521 catgatctgc gcaatcttac cctgattggg aattcatttg gcggcgccgt ttcgctgttg
     2581 gtttccttgc gcctttgtga agagaattca ggaaggttaa ggcggctaat actgatcgac
     2641 agtggtggtt acaatctcca tctgcccaca catctcaaga tgctgcgcac tccgcttctc
     2701 ggctcgctga tgatccacct cttgccgccc aagcttgcgg cgctgacggt gctgaaagat
     2761 tcttactatg acgatcgcaa gatcacgagg gagcaaattg cggcttacgc gagcccgatt
     2821 gcggaaagtg gtggccgcca tgcgctattg gaaacttcga gacagatcat cccgccgaat
     2881 tacgaagagc tgatcaagaa atatccgaag atcgtggtgc ctaccttaat catctggggc
     2941 cggcaggata aagttattcc cctttgcatc ggcaagatgc tggatgcggc acttccggat
     3001 tcgaagttag tagtaatcga tcactcagct cacgtacctc aggaagaaac gccgcaggca
     3061 gttgtgccgc tagtgttgga tttcctccga agttaagtta gccacaggtt cacgcagata
     3121 acacggatga ttgtaagaac ggtagccacc gccttaatcg gatgacaagg aaatgatgca
     3181 aggatagaaa cctctcacaa ctctttacgc tttcggttta tgtcctgatc aatgttatcc
     3241 gcgtaaatct gtggccaata gaaagagcgg cggcaagccg gccttcctga ctgagcattt
     3301 ctgtcaaaat cggcattatg gctgggtgtc cagcagaatc cactccacaa tcgaaagcgg
     3361 caggctgcca ttcttcagca agtcgtcatg aaactgcccc aggcggaagt ttgcgccttg
     3421 tttgtcgcga tacctgccca gcagtcgcat gatctgccac ttgcccacca tgtaagtgat
     3481 cttctgcgtc ggactcgcgg ccgcgccggc tgcttcgccc gtcgccgctt cgcgatcgag
     3541 tccgcctgct tccatgaaat aattcaccgc ttgctcaaag gtccacttgc ccgtatgcag
     3601 gttcacatca acgccgatgc gcgccgcacg atatcgcgat agcctcagca cttgtccgtt
     3661 gcccgccgaa ttgagcggat acaatccgga ccgtagcaac atctcttcgc catagagcgc
     3721 ccaaccttca atgaagatgc cgtcgctatg ttgtctcctg atctcattgg acaaatggtt
     3781 ggcgatcgag agttgcagga agtgtccagg tattccttcg tgcccgagga ttggccgcgg
     3841 gtcctcaata gctgcgcgaa tgtagaagtt cttactgtcg cggttgtagg tcgggatgaa
     3901 atagaaacca ctattgtctt tgtcatacac cccgggagga ttcatgaaac cgccgggact
     3961 cgtcggtttg aaagcttccg gcagttgccg aattgtgaac ggtccaatgt aatcaggaat
     4021 cgtgatcagc cgattcgtct tgagaaaact aatcatctct tcctgccggc tctcgtaagc
     4081 ttttaagaaa gcctgctgat cggcgggaat aactttgctg cgtgccggat taggatcggc
     4141 gagcgctgga tctgacagct gcgactccaa gccgcgataa cgcgccagct ctgcctgccc
     4201 gagcatctcg acctgcttag catcgagcgg cagcaggtag atgtttttca gaatgtagtt
     4261 gtagttttcc tcgcccatcg gtttgaacgc caccatcccg ggcaagcgcc gttcgagcca
     4321 gtcggcgtaa ccgtgcagtg caacgagcgc ggcatcgcgc gctttcacca gctcattccg
     4381 ttctgccgca ttgagacctg tggcgagtgt catcaaactt tcgttgaaga gactgtcgat
     4441 cgagcgggct gattcaatgg ccagttgagc gtagagtcgc accggcttga tgaggttttg
     4501 tttgcctctt tccagcaatt gcggcatttg ccgcaggcgc tcggtggcgg cgagggcgcg
     4561 attacgcggc gtgtcgtact ccttcttgag gagcgaaaaa atgccgttgc tacattcgtt
     4621 cacgtaaacc tgcgggtcgc tctgttcaaa gtccattacg cgattgaaga aaaccacgcc
     4681 atcgatttgt gcgcgaaaga gcatccagtc gatgcgatcg tctttgctcc agccgctcgc
     4741 tggtatcgca tcgaccttgg cgagtatttc cttcgcgtgc aggcgccgag ctaaaaccgc
     4801 ggagagcgag tagtcagtta gacggttgtc ccaggtgtgc aagccggcat cgcttgaagc
     4861 tacgggaaaa ttctcgttgc gccagttgta ataatcggca gccagttttt gcaggtctgc
     4921 gggggtcttc ggttgttgag cactcacggg aatgaccgag ttagaaatta gtacaaaaac
     4981 aaagaccacg ataaataaac gcttcatgct ttggttacct cgctaatagc gggagcgcgc
     5041 agtaggtttt taagtaatgc ttcgtgagca actctaacac acggtttaca acttgaatcg
     5101 ccgcgtttac gcggcagccc gaggagggca aataggccag ccccgacacg tcggcaatca
     5161 catacttgac tcaccaggca atgtcatcgg gtttgggttc caagagcgcc gcaggcgcgg
     5221 cattcaatag ccgcggccgt aaggccgtgg attcaaggct gaagatgaaa tcgaggcccg
     5281 cagggtcggc actcctctgc actgtgatac gggtttac
//
LOCUS       DASUEX010000384         2125 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1108373, whole genome shotgun sequence.
ACCESSION   DASUEX010000384 DASUEX010000000
VERSION     DASUEX010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1108373"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            32..1438
                     /locus_tag="VFV61_06435"
     CDS             32..1438
                     /locus_tag="VFV61_06435"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX4900153.1"
                     /translation="MKPNHELVRGLGPIAAISINVGNIIGTGIFLKARVMTCNVGTPW
                     KVIAAWIAAGLLALAGALTYAELTTMRPNAGAEYVITKDAYGRVWGFLNGWSQFLISR
                     TASAAALAVGFAIFMNNLMDGKLKAVYYTFTMPLFGHTVELGSLTIIALITLAVTTLI
                     NCAAVSVSGQVASLITVLKVLLVLGVGIAAFFYQGGAWGHFGLASTAPCTGEVTGGGL
                     FAFGAAMVGALWAYDGWNNISYMAGEVKHPDRNLPLALIASMLIIIVLYVVVNVSYYY
                     VMTPTEVASVDANASSVAAEAIRKMLGPLAVTLMAAVMMLSSWGSLQSSILGTARIPY
                     AMARDGVFFQSLARVSKNTKVPVISLVVQGIWAAVLTLSGSFDQLTDMAIFAFWLFYG
                     LVAASVFVFRWREPNANRPYKTWGYPVVPGLFVLTTICLIGITIFNYPIRSLAGLAII
                     AAGLPVYFYFSRRNPERL"
     gene            complement(1593..>2125)
                     /locus_tag="VFV61_06440"
     CDS             complement(1593..>2125)
                     /locus_tag="VFV61_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900154.1"
                     /translation="VVYRLPEPDLKTGARAKSKATASTQAETFRLRYPDGSHDAEALL
                     VHPQTGNIYIITKIAFANPGIYEATAPIETGRTITLNRIGTLEVPSLFGGIITGGAIS
                     PDGRRVAFCDYMQGYEAVLENANSAFDTIWKRPVASISLGSRKQGEAITYRLDGKALL
                     MTSEGSPMPLFQVVRR"
BASE COUNT          439 a          577 c          581 g          528 t
ORIGIN      
        1 aacctgaaac ttgaaacttg gaacctgaac cttgaaacct aatcacgagc tagttcgcgg
       61 cctcggaccg atcgccgcga tttccatcaa cgtcggcaac atcatcggca ctggcatctt
      121 tctcaaagcg cgggtgatga cctgcaatgt gggtacgccc tggaaagtga tcgccgcctg
      181 gatagccgca ggtctgctgg ccctcgccgg cgctttaact tatgccgaac tgacaactat
      241 gcggccaaac gctggcgctg agtacgtaat cacgaaagat gcttacggtc gcgtttgggg
      301 ctttctgaac ggatggtccc agtttctgat ctcccgcacc gcttccgcgg cagccctggc
      361 ggttgggttc gcaatcttca tgaacaatct aatggatgga aaactcaagg ccgtctatta
      421 cacgttcacg atgcccctgt ttggccatac ggtggagttg ggcagtttga caataattgc
      481 cctgataact cttgccgtca caactttgat taactgtgct gctgttagcg tgagcggaca
      541 agttgcctcc cttattactg tgctaaaggt cttattggtg ctcggcgtcg ggatagcagc
      601 cttcttttat cagggaggcg cttggggcca cttcgggtta gcgagcacgg ctccctgcac
      661 tggcgaagtg accggaggcg gcttgtttgc ttttggcgcg gcgatggtgg gcgcgctctg
      721 ggcttacgat ggctggaata atatttccta tatggccggc gaagtgaaac acccggaccg
      781 caatctaccg ctcgctttga tcgccagcat gctcatcatc atcgtgctct atgtggtcgt
      841 gaacgtgagc tactactacg taatgacgcc gacggaggtc gcgagtgttg acgccaacgc
      901 atcctcggtt gccgcggagg cgattcggaa aatgctcgga ccattggcag tgacattgat
      961 ggccgcggtg atgatgctct catcctgggg ctcgctgcag agctcaattc tggggacagc
     1021 ccgtattcct tatgcgatgg cgcgcgatgg agttttcttt cagagcctgg cgcgcgtgtc
     1081 aaaaaataca aaggtgccgg taatctcgtt ggttgtgcag ggtatctggg cggcggtgct
     1141 gacactttcg ggttcattcg accagctgac ggatatggca attttcgcct tctggctctt
     1201 ctatggactc gttgcggcat cggtgttcgt gtttcgctgg cgcgaaccca acgccaaccg
     1261 gccgtacaaa acgtggggct atcctgtggt gccgggactg tttgtcttga cgacgatctg
     1321 tctgatcgga ataacgatct tcaactaccc aattcgatct ctagccggct tggcaattat
     1381 tgcagcaggg ctaccagttt atttttactt ttcgagaagg aacccggaaa ggctttgatt
     1441 gattgcggag ccagctccca ggaaacctct cagtcaggaa ggtaatgctc agtctggaag
     1501 gcgggcttgc ctccggctaa gcttgaatgc cattccacaa acggcggtaa accgcccttc
     1561 cgcctgtgct tatatcggca ttcgagttca gtctaccgcc tcacgacctg aaacaacggc
     1621 atcggcgaac cttcactcgt cattagcagc gccttaccat ccaatcgata agtgatcgct
     1681 tcaccctgct tgcgactgcc cagggatatc gacgctactg gtcgcttcca gatcgtatcg
     1741 aaagccgagt tggcgttctc aagtaccgct tcataaccct gcatgtaatc gcaaaaggcc
     1801 actcgtcgcc catcaggcga aatggcgcca ccagtaatga tcccgccaaa caagctcggc
     1861 acttcgagcg tgccaatgcg attgagcgtg atcgtccgcc cggtttctat cggcgccgtc
     1921 gcctcgtaaa ttcccggatt cgcaaaagcg atcttcgtaa tgatgtagat gtttcccgtt
     1981 tgaggatgaa cgagcaaagc ttcggcgtcg tggctgccat ccggataacg caagcgaaag
     2041 gtctcggctt gagtacttgc tgtagctttg ctcttcgcac gagctccggt ttttagatct
     2101 ggttctggca gtcgataaac gacaa
//
LOCUS       DASUEX010000385         2075 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1108470, whole genome shotgun sequence.
ACCESSION   DASUEX010000385 DASUEX010000000
VERSION     DASUEX010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1108470"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="VFV61_06445"
     CDS             complement(<1..189)
                     /locus_tag="VFV61_06445"
                     /inference="COORDINATES: protein motif:HMM:NF042755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6677 family protein"
                     /protein_id="HEX4900155.1"
                     /translation="MQKERLKDRTPDETPSRAWAMGAAGWLVPGAGHLLQGHWVRALI
                     LGGAVWVCFVCGLMMGGHM"
     gene            complement(352..1533)
                     /locus_tag="VFV61_06450"
     CDS             complement(352..1533)
                     /locus_tag="VFV61_06450"
                     /inference="COORDINATES: protein motif:HMM:NF018743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HEX4900156.1"
                     /translation="MKKRIPVKLGLERLLETQAGLARGARVGLICNQASVNHDFHHAA
                     DLLFAHPEVNLTALFGPQHGIRGDVQDNMIETSHAVDRVTGLPIHSLYSETREPTEAM
                     LENVDVLVFDMQDVGCRIYTFAYTMANCMRAAKEFQKKVVVCDRPNPITGQGVAGNVL
                     ELEQASFVGQFPIPTRHGMTLGELARLFNEHFGIGCDLEVLAMDGWAREYWQDETDAP
                     WVMPSPNIPTIDSATVFPGTVHFEGTQVSEGRGTTKPFELVGAPYVEPEEYARALNEL
                     HLPGVFFRSCVFRPTFQKHGGVSCGGVQIHVLNRNDFEPVIAGVAMVKLAFDMYPNDF
                     RWKEPPYEYVYDRNPFDVIAGTSSLRVSFEQGDSLKSIEAQWERPLAEFKKLRERYLL
                     Y"
     gene            complement(1584..>2075)
                     /locus_tag="VFV61_06455"
     CDS             complement(1584..>2075)
                     /locus_tag="VFV61_06455"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX4900157.1"
                     /translation="ICNAGSWEGAPVEEMSEALWDKTMQVNLRGTWTLCQAAVPVMKQ
                     QHFGRIVIVSSTAGQRGEANYSNYAASKGAQIAFTKSLASELGQFGINVNAVAPGWVE
                     TDMSAAALDHADRKAVASSIPVGAIATADDIARPIVFLCSDWARHITGEILNINGGSV
                     LCG"
BASE COUNT          499 a          603 c          498 g          475 t
ORIGIN      
        1 catgtgtcca cccatcatca gcccgcacac aaaacaaacc cagaccgcac caccgagaat
       61 taaggctcgc acccagtgcc cttgcaataa gtggccagca ccaggaacca accagccggc
      121 tgcccccatc gcccacgcac gtgatggggt ttcatcggga gtcctatctt tcaacctctc
      181 tttctgcaac tcatcgcctc caactcaaca gtttctcgtt aaggtattac gtaaactact
      241 actcgatcag tttcgactcg gcacctcaaa cccagtgggt ccttcacgtt ttttctgagg
      301 agtcgtggcg ccgacccttc actggctacc ttaacagcca acacctcaga atcaataaag
      361 caagtaccgc tctctgagct tcttgaactc ggccaagggt ctttcccact gagcctcgat
      421 agatttgagc gaatcgcctt gttcgaacga tacacgcaaa cttgaagtgc ccgctatgac
      481 gtcaaaagga ttacgatcat agacgtattc atagggtggt tctttccagc gaaagtcgtt
      541 gggatacata tcgaaggcca gcttgaccat ggccacaccc gcgatgactg gctcaaaatc
      601 attacggtta agcacatgga tttgcacccc accgcaactg acaccgccgt gcttttggaa
      661 cgtcggtcgg aaaacacaac tgcgaaagaa gacaccggga aggtgaagct cattcagagc
      721 tctagcgtat tcctcaggtt ctacataagg tgcgccaacc aattcgaagg gctttgttgt
      781 tcctcttccc tctgagactt gcgttccctc aaagtgaacc gttcccggga agacagtcgc
      841 cgaatcgatc gtcggaatgt tcggcgaggg cataacccag ggcgcgtcag tttcgtcttg
      901 ccagtattcg cgcgcccagc cgtccatcgc tagaacctcc aggtcacaac caattccaaa
      961 gtgttcatta aacagtcgcg ccaattcgcc gagagtcatt ccatgtctcg tgggaatggg
     1021 aaactggccg acgaatgacg cctgttcgag ctccagcaca ttcccggcta ctccctgccc
     1081 ggtgattgga ttgggccgat cacaaaccac aacctttttc tgaaactctt tcgccgcacg
     1141 catgcaattt gccatggtgt aggcgaacgt atagatgcgg caaccaacat cctgcatatc
     1201 aaaaacgagg acgtccacgt tctcaagcat tgcttcagtg ggttcacgcg tctcgctgta
     1261 gagcgagtgg atcggcaatc cggttacccg atcaaccgca tgcgaggtct cgatcatgtt
     1321 gtcctgaaca tcgccgcgaa taccatgctg gggaccaaac aacgccgtca ggtttacttc
     1381 tgggtgagcg aagagcaggt cggctgcgtg atggaagtca tggttaacag acgcctgatt
     1441 acagattaga ccaactctcg cgccgcgcgc taagccagct tgcgtttcaa gtaaccgttc
     1501 taacccgagt tttactggaa tccttttctt cattccttaa gagactagat gagcaagaga
     1561 ttagttaaag taaaagctga actttagccg caaagtacag aaccgccgtt gatgttaagt
     1621 atctcgcccg tgatgtgccg cgcccagtcg ctgcaaagaa aaacgatggg cctggcgatg
     1681 tcatcggcag tagcgatcgc tccgactgga atactgctgg cgactgcttt cctgtctgcg
     1741 tggtccaggg cggcagcaga catgtcggtc tcgacccagc ctggcgcaac tgcgttaaca
     1801 tttatgccaa attgaccaag ttcagaagca agcgacttcg tgaaagcgat ttgcgctcct
     1861 tttgaagcag catagttcga atagtttgct tctccccgtt ggcccgcagt cgaagagacg
     1921 attacgatgc gtccaaagtg ctgctgcttc atcaccggca cggcagcctg gcagagcgtc
     1981 caggtgccgc gcaagttgac ctgcatagtc ttgtcccaca gtgcttcaga catttcttca
     2041 acgggcgcgc cctcccaact ccccgcattg cagat
//
LOCUS       DASUEX010000386         2500 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_873825, whole genome shotgun sequence.
ACCESSION   DASUEX010000386 DASUEX010000000
VERSION     DASUEX010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_873825"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="VFV61_06460"
     CDS             <1..464
                     /locus_tag="VFV61_06460"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HEX4900158.1"
                     /translation="NLQMMAVNKEQLDLEKKAQDIQSRIDAIKRLRESQQGPGSVLRE
                     IKTRFDAVPGLYLKSIEQKDGELIVKGESPNEVSVTRFGQSMEFSSGLFTNLNIETQR
                     ELAKGSSSPSDPGDVTVAPTEVVSFTVKCNFAKSQTSAAPNPPANQVALKK"
     gene            520..1158
                     /gene="pilO"
                     /locus_tag="VFV61_06465"
     CDS             520..1158
                     /gene="pilO"
                     /locus_tag="VFV61_06465"
                     /inference="COORDINATES: protein motif:HMM:NF016253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HEX4900159.1"
                     /translation="MRFGNLKSVPWYLRLMVFVFVAGVFYAAFWYFVTSGTRKETKAM
                     EAEIAQLLPRNAQAQIASQRLNEFRAIYKARQEEYDELNALLPEQRELTMVLQGLQDR
                     ARNTGLVFRRFNPKEDVQQENYNGKKIEVGVSSSFASLRAFFEQVAHYQRIVSITNFE
                     LKQIDKQSPKQTVEARFDLTAFYVSAESLQKQSNVKPGAPAGQAPAPQAAAK"
     gene            1190..1831
                     /locus_tag="VFV61_06470"
     CDS             1190..1831
                     /locus_tag="VFV61_06470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900160.1"
                     /translation="MMHSTNKLRFTSAAIALSLAFGIGATTNAQDQKSVVDKPSAKRV
                     SDSGRDPFRKYEPPRPGKKSQNLVAIPSIQERIAQYRAQKAAAMSARMAAPKPTTALL
                     LSEMQVVGISRSPRGYAAIVEATPINLSYVLYPGERFYDGQLVAIEDNRLIFRRETVF
                     VDGRRERSVEIKSLRQPGSPTDMTTTPSSAAAPAAAESDKKSDDTSAQANKPK"
     gene            2038..>2500
                     /locus_tag="VFV61_06475"
     CDS             2038..>2500
                     /locus_tag="VFV61_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900161.1"
                     /translation="MPYQSPILAAIKRTALVLLCLSLVGANTLAGTTAPRPATSKAAT
                     LKLVNYIPLSAQAEARLAAEIVAAHDSENQDPAVAQNQSQTKPGISPGTASQSDQGRQ
                     YGQPGFVGEPINLNVVNADIRDILNYITEQYGVNFVIDSSVRSVPVTVNVQD"
BASE COUNT          618 a          701 c          668 g          513 t
ORIGIN      
        1 tcaacctgca aatgatggct gtcaacaaag agcagctcga cctcgagaaa aaagcacagg
       61 atattcagag ccgtatcgac gctatcaagc ggctgcgcga gtcgcagcag ggtcccgggt
      121 ctgtcctgcg tgaaatcaag actcgtttcg atgcagtacc cggactctac ctcaagagta
      181 tcgaacagaa agacggcgag ctgattgtga aaggcgagtc gcccaatgaa gtttcagtta
      241 ctcgtttcgg gcaaagcatg gaattctcgt ctgggctctt cactaacctg aacattgaaa
      301 ctcagcgcga actggcgaaa ggttccagca gcccgagtga tccaggtgac gtaacggtag
      361 cgccaacgga agtggtttcg tttaccgtta agtgcaattt tgcgaagtcg cagacctcag
      421 ccgccccaaa cccgcccgca aatcaagttg ccctaaagaa atagggccgt tgactctaac
      481 caaacccgcc cagttctggg gggaaggaaa tgaaaagcga tgagattcgg taacctcaaa
      541 tcagtgccct ggtacctgcg acttatggtc tttgtatttg ttgccggcgt cttctacgcg
      601 gcgttctggt atttcgtcac cagtggcacg cgcaaggaaa ccaaagcaat ggaagctgag
      661 atcgcgcaac tgttgcctcg caatgcccag gcgcaaattg cctctcaacg gctcaacgag
      721 tttcgcgcga tttataaagc gcgccaggaa gagtatgacg agttaaatgc cctcctcccg
      781 gagcagcgcg agctgacgat ggtcttacag ggactgcaag accgcgcccg caatacggga
      841 cttgttttcc gccgcttcaa tcccaaggaa gacgtgcagc aggaaaacta caacgggaag
      901 aagatcgagg ttggcgtgag cagctcgttt gcgtcattgc gggccttctt cgagcaggta
      961 gcgcattacc agcgtatcgt ttcgattacc aattttgaac tgaagcagat cgataaacag
     1021 tctccgaagc aaacagttga agcgcgcttt gatctgacag ccttttatgt gtcagcggaa
     1081 agcctgcaga aacagagcaa tgtgaaaccc ggtgctcccg ctggtcaagc gcccgctcct
     1141 caggctgctg cgaagtagca tcactcaagc gcacgtttaa ggaagaaaca tgatgcattc
     1201 caccaacaaa ctcagattta cttccgcggc aatcgcactc agcctggcat tcggaattgg
     1261 tgcgacaaca aatgcccagg atcaaaaatc cgttgtcgat aaaccgagcg cgaagcgcgt
     1321 ctctgattcc ggtcgcgacc cgtttcggaa gtatgaacca ccgcggccag gaaagaagtc
     1381 tcaaaacctg gtggcgattc cttccattca ggagcgcatt gcccagtatc gagcgcagaa
     1441 ggccgcggcg atgagtgctc gcatggcggc acctaaaccg acgaccgcgc tgttgcttag
     1501 tgaaatgcaa gtggtaggaa tctcgcgttc gccgcgcggt tatgcggcta tcgtcgaggc
     1561 cactcccatc aacttgtcgt atgtgctgta tcccggcgaa cgtttctacg atggccagtt
     1621 agttgccatt gaagacaacc ggctgatctt tcggcgtgag actgtttttg tagatggacg
     1681 tcgcgaacgg tcggttgaga tcaagtcgtt acgccagccc ggctcgccga cggacatgac
     1741 aaccactccg tcctcggccg ccgctcctgc cgccgctgag tcagacaaaa agtctgatga
     1801 cacctcagct caggccaaca agccgaaata actttctcgc tggcgctaat tgaaatcggc
     1861 gccagccttt tcctccgtcg caacccgaac ctctcagtaa aactccgaac gtcggattgc
     1921 gcggaattga caatcaaccc ccggacgcag ccgcaagtgc ggcgcgcgct ttagttgcac
     1981 tggcaaatga gcgtagcgcg tggcgggcac gtccaaaatt cccttaagga gggaacgatg
     2041 ccttatcagt caccaattct tgcagccatt aaaaggaccg cgctcgtcct gttgtgtttg
     2101 agcctcgtcg gcgcgaacac tctggctgga actaccgctc cgcgcccggc aactagcaaa
     2161 gctgccacgc tcaaactagt gaactacatc ccgttgtcgg cgcaagctga agcgcgcctg
     2221 gccgccgaaa tcgtggcggc acatgattct gaaaatcagg atccggccgt cgcgcagaat
     2281 cagtcgcaaa caaagcccgg catatcccct gggaccgcta gccaatcgga tcagggccgg
     2341 cagtacggac aaccgggatt cgtgggcgag ccgatcaacc tgaatgttgt aaacgcagac
     2401 atcagggaca ttctcaacta cattaccgaa caatacggtg tgaactttgt aatcgattcc
     2461 tccgtgcgtt cggtgccggt gactgtgaac gttcaagacg
//
LOCUS       DASUEX010000387         4497 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_638341, whole genome shotgun sequence.
ACCESSION   DASUEX010000387 DASUEX010000000
VERSION     DASUEX010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4497
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_638341"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1041
                     /locus_tag="VFV61_06480"
     CDS             <1..1041
                     /locus_tag="VFV61_06480"
                     /inference="COORDINATES: protein motif:HMM:NF025877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Wzt carbohydrate-binding domain-containing
                     protein"
                     /protein_id="HEX4900162.1"
                     /translation="FTGIENVFMNASLMGFSRSETEALLPEIERFAEIGPFIYQPVKT
                     YSSGMYVRLAFSVAISAAPQILIIDEALAVGDAVFQHRCMRRLKEMQESGVTIFFVSH
                     DLASVRALCSRAILMHAGHAVADGKPTTVLNHYQKIIMARETAFDREELTETANPEQE
                     IGLVLRDMPKVECAYRHGDGSAEVVRVELLDNADRSIVLVETGESVTIRILVRINREL
                     NEPIFGFLLRNRHGIHLYGTNTDLQQVRIGKARRGEIVEVAFPFDCWLAPDSYSVTVA
                     VHSREGVSYDWLDGVLLFQVVSQVPMDGVANLNALVNIRRFNLATDGAREALAHSSSQ
                     HDGAVVGRDIAN"
     gene            1182..3518
                     /locus_tag="VFV61_06485"
     CDS             1182..3518
                     /locus_tag="VFV61_06485"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEX4900163.1"
                     /translation="MNSTETTEVSGRTQPVAEESRSEPESAPKLGEFVSATGYKFFAR
                     PDTTDSAIFQLVVDENEYNLPPRFQADDIVIDIGAHIGGFSYAVLERGAGKVYAFEAH
                     PANHAIASRNLERFGKRINCQQRAVWRSDQPSQTLYNESLVGNANPGGVSLLWNEAGN
                     AVETISLDEILAEASDGFRKPIRLLKIDCEGSEYPILFTSNQLEIVEEICGEYHEVPP
                     ERIPERAKVSKYEHFDRFALRDFFEDRGWSIILEPKVDQLGLFHARSQSSSMSPAPDF
                     NVAEFMSEVREAVRLREARGDTSFINASTELFKLLREEGFFDEPVAEFDSHLTEWTPA
                     GELPELRLHPEFQPSSDDRYHVNDLLQYHDREFIWNAYKAILKREPDESGLRDFLTRL
                     RNGSRNKIDILASLRSSAEGKRANVSIEGLAIPAIIRKVYRVPVLGYVAETAVAIARL
                     PKLVRSQRQFETHLVAQQDRLAGHIKQTNHLLVSKIEKLRLEARSDADSLRADSVAFR
                     GSFRESLLKLAKLQKHIARLQHEQVSALFRDQEPLRSAQATATKTGNNRPAEAAEFEQ
                     LEASLSEFLRGDREAVKTDLDFYLALLKESKATKEILDLGCGSGVWLEMLKESGFQAR
                     GIEANQRLVDAGRQRGLEIIQDDVFEYLKGQPDTSLQAVTAFHLVEHLEFQALFRLLI
                     EIRRVLKPGGLLILETPNPKNLVVGACNFYADPTHRQPLFPETLQFLLEAVGFVQTRI
                     TYLHPTEGSPFNPAEPGSRELHIWLFGPRDFAAIGWKS"
     gene            3546..>4497
                     /locus_tag="VFV61_06490"
     CDS             3546..>4497
                     /locus_tag="VFV61_06490"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX4900164.1"
                     /translation="MARFAIVTSHLTSGDAVSNDVVTMQRVLENHGCEARLYAGSADF
                     EEPRVWPLAEIGEFLQHVDDVLIYHHSFGWEPGMALLRELKCKTIIKYHNVTPPEFFA
                     GVSSWHEEKCAEGLRQLEFIATAGYEAYFSDSAYNMQQLLDLGADSARNFVVPPFHHI
                     DRLDTICGDRETLETYRDGKTNILSVSRVAPHKNQEALIEAFAIYHHYYNINSRLLII
                     GREETAFETYSARLRELINFLLLDGTVVFGGEVSDAALKAYYLLSNAFLFASKHEGFS
                     VPLVEAMAMKVPIVSYGAAAVPETVAQAGIVLDELDPEKLA"
BASE COUNT         1065 a         1155 c         1210 g         1067 t
ORIGIN      
        1 ttcactggta ttgaaaacgt gttcatgaat gcttcgttga tgggtttttc gcgaagcgag
       61 actgaagcct tgttgccgga aattgagcgc tttgcggaaa tcgggccgtt catttatcaa
      121 ccagtcaaga cctactcaag cggcatgtac gtgcgcctcg cgttttccgt agcgatcagt
      181 gcggccccgc agatcctcat tattgatgaa gcgctcgcag tcggcgatgc tgttttccag
      241 catcgttgca tgcgccgcct gaaagagatg caggagagcg gggttacgat cttttttgtt
      301 tcgcacgatc tggcttcggt gcgagcgctt tgttcgcggg cgatcctaat gcatgccggt
      361 cacgcagttg ccgacggaaa gccgaccacg gttctcaacc actatcaaaa gatcatcatg
      421 gctcgcgaga ctgcctttga tcgcgaagag cttacggaaa cagcaaaccc ggaacaggag
      481 atcggcctgg ttctaagaga catgccgaag gttgaatgcg cttaccggca tggtgacggc
      541 agcgcggaag tggttagagt tgaactactc gataacgctg accgttcaat tgtgctagtg
      601 gaaacaggag aatctgtcac gatccggatt ttggttcgca tcaatcggga actgaatgaa
      661 cctatctttg gcttcctgct gcgcaatcgt catggcatcc atctctatgg aaccaatact
      721 gacttgcagc aagtcaggat cggcaaggct cgacgcggag agattgttga agtcgcgttt
      781 ccctttgatt gctggcttgc ccccgactcc tacagtgtca ctgtcgccgt gcacagtcgc
      841 gagggcgtta gctacgactg gctggacggt gtattgctct ttcaggtcgt gagccaggtc
      901 ccgatggatg gggttgcaaa ccttaacgct ttggtgaaca ttcgcaggtt taatctcgcg
      961 actgacggcg cgcgcgaagc actggcacat tcgtcaagtc agcacgatgg ggcggtagtg
     1021 ggccgtgaca ttgccaattg acaattgccg atttggattt gttcctttcg cgacgggcca
     1081 ttggcaatcg tcaatcggca atcggcaatg gacagtcggc ctgacttacg gaaagtgcgg
     1141 cagcgagtta gaatcgattc atccacgcca actggaaatc aatgaacagt actgaaacaa
     1201 cagaagtatc gggacgcact caacccgtcg ccgaggaatc gagaagtgag ccggaatccg
     1261 ctccgaagct gggcgaattc gtttccgcta cgggatataa attttttgcc cggcctgaca
     1321 ctaccgattc cgcgatcttt caactcgtag ttgatgaaaa tgaatataac ttgccgccgc
     1381 ggttccaagc tgatgacatt gtgatcgata tcggcgctca cattggcggc ttctcttatg
     1441 cggtcctgga gcgaggtgcg ggaaaggtgt atgcctttga agcccatccc gcgaatcacg
     1501 cgattgcgtc tcgaaatctt gaacgcttcg gtaagagaat aaactgccag caacgagcgg
     1561 tctggcgctc agaccaacct tcccaaacgc tctataacga gagtctcgtc ggcaacgcca
     1621 atccaggcgg cgtttcgttg ctttggaacg aagctggtaa cgcagtcgaa acaattagtc
     1681 tggacgagat tctcgcggaa gcgtctgatg ggtttcgcaa accgatccga cttttgaaaa
     1741 ttgattgtga agggtcggag tatcctattt tattcacgtc aaaccagctc gaaatcgtcg
     1801 aagaaatctg cggcgaatat cacgaagtac ctcctgagcg aatccctgaa cgcgccaagg
     1861 tgagcaagta tgaacatttt gaccggtttg ccctcagaga ttttttcgaa gatcgaggat
     1921 ggtcgatcat tctggaacca aaagtagatc agctcggtct ctttcacgcg cgctcccagt
     1981 cgagctcgat gtctcccgcg ccagacttta acgtggccga gtttatgtca gaagttcggg
     2041 aagcggtgcg cttgcgagaa gccagaggag atacctcgtt catcaatgct tccactgagt
     2101 tgttcaagtt gctaagggaa gaaggctttt ttgacgaacc ggtggccgag tttgattcac
     2161 acctcactga gtggacgccg gccggggaat tgccagaact tcgcttgcac cccgagttcc
     2221 aaccaagctc ggacgatcgc taccatgtca acgacttatt gcagtatcac gatcgcgagt
     2281 ttatctggaa tgcctacaaa gcgatcttga aacgcgaacc cgatgaatcg ggtctgagag
     2341 atttcctcac aagattgagg aatggaagtc gcaacaagat cgacattcta gcgagcctac
     2401 gttcctctgc tgagggcaag cgagccaacg tttcgattga aggcctggca ataccagcga
     2461 tcatcaggaa ggtctaccgc gtcccagtac tcggttatgt cgccgagact gcagttgcta
     2521 ttgctcggct gccgaagtta gttcgcagtc agagacaatt cgaaacccac ctggtcgcgc
     2581 agcaagacag attggccggc cacatcaagc agacgaatca tctgctagtc agcaagatcg
     2641 aaaagctgcg cctggaagct cgaagtgacg cagattcgct gcgtgctgac tcagtcgcgt
     2701 tccgcgggtc attccgcgag tcgttactca aattagctaa attgcagaag cacatagcca
     2761 ggctgcagca tgaacaggtc agcgctctct tcagagatca agaacctctg cgcagtgctc
     2821 aagccacagc aacaaaaacc ggaaacaata gaccagcgga ggcggcagag tttgaacagc
     2881 tggaggcctc gctatctgag tttctgcggg gcgatcgtga agctgtaaag accgacctgg
     2941 acttctactt ggcgctactg aaggaatcca aggcaacgaa agagatactc gacttgggct
     3001 gcggcagtgg tgtctggctt gaaatgctta aagaatctgg ttttcaggcc cgaggcatcg
     3061 aggcgaatca gcggctggtc gacgcgggca ggcagcgtgg actcgagatt attcaagacg
     3121 acgtttttga ataccttaaa gggcagccag atactagtct gcaagcagtg actgcatttc
     3181 atctggttga gcatctggaa tttcaggcgc tgtttcggtt actgatagag attcgccggg
     3241 tcttgaaacc gggaggattg ctgattcttg aaacccccaa tccaaagaat ctggtcgtcg
     3301 gcgcctgcaa cttctacgct gatccaactc atcgccagcc gcttttccca gagacgcttc
     3361 agtttctgtt ggaagccgtc ggctttgtcc aaacgcgcat cacctatctg catccaaccg
     3421 agggcagtcc tttcaaccct gctgagccgg gctctcgcga actgcacatt tggttattcg
     3481 gaccccgcga cttcgcggcc ataggctgga agtcctagac accttaactc cttcccacat
     3541 ttcccatggc ccgctttgca atcgtaactt ctcatctgac ctccggcgat gctgtcagca
     3601 acgacgtcgt aacgatgcaa cgggtgctcg agaatcacgg atgcgaagcg agactctacg
     3661 cggggagtgc cgattttgaa gaaccacgag tttggccgct ggccgagatc ggcgagtttc
     3721 tccagcatgt tgatgatgtt ttgatctacc accattcttt tggctgggaa ccaggaatgg
     3781 cgctgctgcg cgaactgaag tgcaagacga tcatcaaata tcacaatgtg acgcctcccg
     3841 agttttttgc tggtgtcagt tcatggcatg aagagaagtg tgctgaggga ctacgtcaac
     3901 tggaattcat cgcgactgcg ggttacgaag cttacttctc tgactccgcc tacaacatgc
     3961 aacagttgct ggatctggga gctgattccg cccgcaactt tgtagttccc ccctttcatc
     4021 atatcgatcg gctggatacg atctgcggtg atcgggagac gcttgagact tacagagacg
     4081 gcaagacgaa catactcagc gtcagtcgcg tagctccgca taaaaatcag gaagctttga
     4141 tcgaggcttt cgcgatctat caccactact acaatatcaa tagtcgtctc ctaataattg
     4201 gacgcgaaga aacagccttc gaaacttatt cggcacgact ccgcgagttg atcaactttc
     4261 ttctcctcga tggcacggta gtttttggcg gtgaagtttc cgatgccgcc cttaaggcgt
     4321 actacctcct cagcaacgct tttctatttg caagtaagca tgagggtttt tcagtaccac
     4381 tggtggaagc gatggcaatg aaggtcccca tcgttagcta cggtgcggcg gcggttcctg
     4441 agactgttgc ccaggcagga atagttctgg acgaattgga tcctgaaaag ctggccc
//
LOCUS       DASUEX010000388         5362 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_398066, whole genome shotgun sequence.
ACCESSION   DASUEX010000388 DASUEX010000000
VERSION     DASUEX010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5362
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398066"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            359..2197
                     /locus_tag="VFV61_06495"
     CDS             359..2197
                     /locus_tag="VFV61_06495"
                     /inference="COORDINATES: protein motif:HMM:NF025104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900165.1"
                     /translation="MPFSSLNLLSVAVAFGLALALTPFVRAFARRIGLVAKPKSDRWH
                     KKPTAMLGGVAIWLSVVISYLLYFRPNKLFGFDSTNPSVSYSWVIISASSFLFLVGLV
                     DDLIHTKPYQKLIGQIMGSAFVIYYGLALPWTGSNSLNVVLTIFWLIGITNALNLLDN
                     MDGLAAGIAVIGSAFLALSFLSTGQSTEALMMITFAGALLGFLVYNSNPASIFMGDCG
                     SLFVGFFLASSALVNVAGGRSRSLLPVLAVPILVLFIPIFDTTFVTILRKVSGRAASQ
                     GGRDHTSHRLVALGMSEQRAVWMLYSFAALSGILALLVQRMKLDVSLAAIAGFTILLT
                     LLGVYLAGVKVYDQSEEAQALQDNSLYAFLVDVSYKRRIFEVLLDVVLIVLAYWSAYA
                     IKFGAFSGNAAWRLFLQTVPVLVFVKMATFLAMGVYRGLWRYTSLDDLIVFVKAVVIS
                     SVASVLVLLFAFRFAGFSRAAFVLDGILMLVLLAGSRMAFRLFRQLLPKAPIAEGRRV
                     FIYGAGDGGELLLRELQNNRELRYTPVGFLDDDPAKKGKLIHGLKVFAANGDLTQVCR
                     ELGADELIISSAKVSEERVQEIASTCQAQEIAVKRMKITIEDVKQI"
     gene            2208..3503
                     /locus_tag="VFV61_06500"
     CDS             2208..3503
                     /locus_tag="VFV61_06500"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX4900166.1"
                     /translation="MAELLQIDTSRMVTRVALFLTLVLAFVVSTFGIRAYVGSTMAEY
                     LSSEEGGLEMPRRAVAWAPRDPHAHWKLGAIMERKLPADQLPQALAEFEKAASLSPND
                     YRFWMDFGTALEQAGEVERAEKVLRRSVELAPSYAYPRWYLGNLFLRSGRYPEAFAEL
                     RRASEADPTFQPQLFNSAWAVYSDDIQALTSAVGSTGTARAGFAQYLIGFQRFDDGIR
                     LWKSLNENERRANREAGEAIVKSLVASQRYHQAADVSNDLAPASVHSAEGKFVDGGFE
                     SGLGYREGSIFGWKVTSTSQAQIGIDPAQGYQSERSLRLLFQVRTRLDAVNVSQLIPI
                     APDTTYELEYAVKTQKLDSADLPTVAVFDEVEGKLLVKSEEAPGGSSDWKVVKLSFKT
                     GPKTQAITIRLQRTACAPEASCPIYGTVWYDNFNLQRRG"
     gene            3472..4956
                     /locus_tag="VFV61_06505"
     CDS             3472..4956
                     /locus_tag="VFV61_06505"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX4900167.1"
                     /translation="MTTSISNVAVNPSLEVAGETTGASGEQTIHRVRHTLASRFVFLG
                     LCVAIVLTALAFGTNHNWALAFFNLGALAIVFLWIMDGWKLGVLRLSRNPLQLPLLGM
                     LLLGLIQLLPLRSAAAVDLLSIPAVKSLSLDPYATTLILADVLTLLVYFAATLVFTDT
                     PHRLHLLVRTITIFGFLLAIFGLTQSFTTSKVFWLRELSQSTAFGPFINRHHFAGYME
                     LTMALPLGMLFSGSLEKDKRFAYIFAVVLMGVALMMTNSRGGVISLVAEIMFLLVLSG
                     FRKRKTHEVLEKSQRIKRAALRAAMALGLIVALFAGVVLLGGEQALSRFAGTVNSEDP
                     TTGRAHFWNVTLDIIKAHPIVGTGLGAFPTVYTRYDSRNGAFRLEQVHNDYLQILSDG
                     GVIGAALGLFFVVMLFRHGFARRESSDDYRGGVALGALAGCFAVLVHSFFDFTLHTTA
                     NALLFLVMAALATMNGRVEQPAKRRRRRRHRHLEDSGPIMIEPT"
     gene            5079..>5362
                     /locus_tag="VFV61_06510"
     CDS             5079..>5362
                     /locus_tag="VFV61_06510"
                     /inference="COORDINATES: protein motif:HMM:NF012239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX4900168.1"
                     /translation="MAHIDAGKTTTTERILFYTGVLHRVGEVHDGAATMDWMEQEKER
                     GITITSAATTCFWKDHRINIIDTPGHVDFTIEVERSLRVLDGAIAVFCSVG"
BASE COUNT         1072 a         1473 c         1495 g         1322 t
ORIGIN      
        1 cgaagagggt ttcgaagacc tgccgcgtcg tataccggac atcaccaaga ttaaagaact
       61 ggtgggcttc gctccgacga tgccacttga tgggatcctg caaagcgtaa tcgatcacca
      121 gagcggccgg ccttcggcac aggcagcgca ataaagctca ggtcggaatg cacccggccc
      181 ttaaaagaat tcctcgagcc cagggggtca ggtccaagag tcccttctgg ctactgctcg
      241 ttcgcgtacc tggcttcggt cgtgtgacct cgctcacttg caatgctccg cgctgatcgt
      301 ctagcttctc ttcgccgcaa gttcaaatta caattcgagc gctcagtctc gctgaacgat
      361 gccctttagc tcgcttaatc tgctgtcagt cgcagtggcc tttggtctgg ccctggcgct
      421 gacgccgttc gtgcgcgcat tcgcgcgacg catcggcctc gtagctaaac cgaaaagtga
      481 tcgctggcat aaaaagccga ctgcgatgct cggcggcgta gccatctggc tctcggtagt
      541 gatttcttat ctgctgtatt ttcgaccgaa taaactcttt ggttttgaca gcactaatcc
      601 gagcgtctcg tacagttggg tgatcatcag cgccagctct ttcctcttcc tcgtcggtct
      661 cgtcgacgat ctaattcaca ccaagcctta tcagaagttg atcgggcaga tcatgggttc
      721 ggcttttgtc atctattacg gactggccct gccgtggacc ggttcaaatt cgctcaatgt
      781 cgtgcttacg attttttggt taatcggaat cacgaacgcg ctcaacctgc tcgacaacat
      841 ggacggattg gcggcgggga ttgccgtcat cggttcggcg tttctggcgc ttagcttcct
      901 cagcaccggc cagtcaaccg aagcgttgat gatgataact ttcgcgggcg ccctgctcgg
      961 ttttctggtt tataactcga atccggcatc gatcttcatg ggcgactgtg gttcgctctt
     1021 cgtgggattc tttttggcaa gttcagccct cgtaaatgtt gccggcggcc gttcgcgcag
     1081 tctcttgccg gtccttgctg tgcccatcct cgttctcttt atcccgatct tcgacaccac
     1141 tttcgtaact atcctgcgga aagtttctgg acgcgcggct tcgcaaggcg gacgggatca
     1201 cacctcgcac cggctggtag cgctcggcat gtcagaacag cgtgccgtct ggatgctcta
     1261 cagttttgca gccctgtcag gaatcctggc cctgctcgta caacgaatga agctggatgt
     1321 gagtctggcg gcgatcgccg gcttcaccat cctgctaacg ttgttaggtg tctaccttgc
     1381 aggcgtcaag gtgtacgacc agagtgaaga agcgcaggcg ttgcaggaca actcgcttta
     1441 tgcatttctg gttgacgttt cttacaagcg tcggattttt gaggtgctgc tcgacgtggt
     1501 gctgatcgtg ctcgcgtact ggagtgctta cgcgatcaag tttggcgcgt tttcaggaaa
     1561 tgcagcatgg cggctgtttt tgcagaccgt gccggtgctg gtgttcgtca aaatggcgac
     1621 cttcctggcg atgggtgtgt accggggttt gtggcgttat accagcctcg acgatttgat
     1681 cgtcttcgtt aaggcagtcg taatcagctc ggtagctagc gttctagttt tactctttgc
     1741 ctttcggttc gcaggctttt cgcgagcggc ctttgtcctt gatggcattc tgatgctggt
     1801 gcttctggcg ggcagccgta tggcgtttcg gctgttccgg cagttgctgc ccaaggcgcc
     1861 aatcgccgaa ggccggcgcg tatttatcta cggcgcgggc gatggtggcg agttgctcct
     1921 gcgagaactg caaaacaatc gcgaattgcg ctacacgcca gtgggatttc tcgacgacga
     1981 tccggcgaag aaaggcaaac tgatccacgg cttaaaggtg tttgccgcta atggcgactt
     2041 gacgcaggtt tgcagagagc tgggagccga cgaactgata atctccagcg caaaagtttc
     2101 cgaagaacgc gttcaggaaa tcgccagcac ttgccaggcg caggagatcg ctgtgaaacg
     2161 gatgaaaatc acgatcgagg acgtgaaaca gatttagaaa cctaccgatg gccgaactcc
     2221 tgcaaataga cacgtcccgg atggtcacgc gagtggccct gtttctaacg ctggtgctgg
     2281 cgttcgtcgt ttccacgttt ggtattagag cctatgtcgg cagcaccatg gccgagtatc
     2341 tgagcagcga agaaggcgga ttggaaatgc cacggcgtgc cgtcgcgtgg gcgccgcgcg
     2401 atcctcatgc acactggaag ctcggcgcga tcatggagcg caagcttccc gctgaccagt
     2461 tgccgcaggc ccttgccgaa ttcgagaagg ctgcgagtct ctcgcccaac gactatcgct
     2521 tctggatgga cttcggaacg gcgctggaac aggccggcga ggtcgaacgt gcggaaaaag
     2581 tcttgcgccg ttccgttgag ctggccccgt cctacgccta tccgcgctgg tacctgggaa
     2641 accttttctt gcgaagcgga cgttaccccg aagctttcgc cgaactgcgc cgcgccagtg
     2701 aggcggatcc gacatttcag ccgcagttgt tcaattccgc ctgggcggtt tacagcgacg
     2761 acatccaggc gctgacgtcc gctgtcggat cgacggggac cgcccgggcc gggtttgctc
     2821 aatacctgat tggctttcaa agattcgatg atggtattcg actttggaag agtttgaatg
     2881 agaacgagcg ccgcgccaat cgcgaagcag gcgaggcaat tgttaagagc ctggtggctt
     2941 cgcagcgcta tcaccaggcg gccgacgttt ctaacgatct cgctcctgcc tccgttcaca
     3001 gtgccgaagg aaaatttgtc gatggcggct tcgaatcggg gctgggttac cgcgaaggat
     3061 caatctttgg ttggaaggtt acctctacat cgcaagccca gattggaatc gatccggcac
     3121 agggttacca gagtgagcgc agcttgcgcc tcttgttcca ggtgcgaacc aggctcgatg
     3181 ctgtcaacgt ttcgcaactg ataccgatcg cccccgacac cacctacgaa ctcgagtacg
     3241 ccgtcaaaac ccagaagttg gatagcgctg atttgccgac cgtcgcggtc tttgacgaag
     3301 ttgaaggaaa gctgttggtt aaatccgaag aggctccggg aggcagcagc gattggaaag
     3361 tagttaagct cagtttcaag acgggaccaa aaacccaggc catcaccatt cgcctgcaac
     3421 gaaccgcctg tgccccggaa gctagttgcc caatctacgg aactgtctgg tatgacaact
     3481 tcaatctcca acgtcgcggt taacccgtct ttggaagtgg ccggcgagac tacgggcgca
     3541 tcaggtgaac aaacgattca ccgggtgcgc cacacgctcg ccagtcgttt cgtctttctc
     3601 ggtctttgcg tggcgattgt gctcaccgct ctcgccttcg gcaccaatca caattgggca
     3661 ctcgcctttt tcaatctcgg cgcactcgcc atcgtattcc tttggatcat ggacggctgg
     3721 aagcttggtg tcttgcgtct tagccgcaat cctttgcaac tcccgctact gggaatgttg
     3781 ttgctgggct taattcaatt gctaccgctg cgcagcgctg ccgctgttga tttactttcc
     3841 attcctgccg ttaagtcgct ctcgctcgat ccttatgcga caactctgat cctggccgat
     3901 gtgttaacgc tgctcgtcta ctttgccgcc acgctcgtat tcaccgacac gccacatcgc
     3961 ctgcacttgc tggtcagaac gattaccatc tttgggtttc tgctggcgat cttcggatta
     4021 acgcaatctt tcacgaccag caaagtattc tggctccgcg aactatcgca gagcacagcc
     4081 ttcggaccat tcatcaaccg ccatcatttc gccggttaca tggaattgac gatggccctg
     4141 cccctgggaa tgctgttctc cggatcgttg gagaaagaca agcgctttgc ctacatcttc
     4201 gccgttgtct tgatgggagt ggcgctgatg atgactaact cgcgcggcgg tgtgattagt
     4261 ctggtcgctg agatcatgtt tctgttggtg ttgtccggtt ttcgcaagcg caagactcat
     4321 gaggtcctgg agaaatcgca acgcatcaaa cgtgcagcct tgcgggcggc aatggcgttg
     4381 ggcttaattg tcgctctttt tgccggagta gttttgttgg gtggcgagca ggccttgagc
     4441 cggttcgccg gcacggtcaa ctcagaagat cccacgacgg gccgcgctca tttttggaat
     4501 gtaacgctgg acataattaa agcccatccg attgtcggta ccggtctggg tgctttcccg
     4561 accgtctaca ctcgttacga ttcgcgtaac ggggcgtttc ggctggagca ggtgcacaac
     4621 gattacctcc agatcttgtc ggatggcggg gtgatcggcg cagcgctcgg actgtttttc
     4681 gtggtgatgc tgtttcgtca cggctttgcc cggcgcgaat cgtccgatga ctatcgcggt
     4741 ggcgtggccc tgggtgcact tgccggatgc tttgcggtat tggtgcacag cttttttgat
     4801 ttcactctgc atacaacggc aaacgctttg ctgtttctgg taatggcagc gcttgccacg
     4861 atgaacggtc gtgtagaaca gccggccaaa cgccggcgtc gccgccgcca ccgtcacctg
     4921 gaagactcag ggcccataat gatcgaaccg acataaatct agttccaagt tccaagttca
     4981 aagcaccaag ttcaaagttc aaagaccaaa gaccaaaaac caaaaaccaa agtggtatgg
     5041 caaacattag tttagacaaa tttagaaaca tcggcattat ggcgcacatt gacgcgggta
     5101 aaaccacgac aaccgagcgc attctttttt atacaggtgt attgcaccgt gtgggtgaag
     5161 tgcacgacgg tgcggcaaca atggattgga tggagcagga aaaagaacgc ggtatcacca
     5221 tcacctccgc tgctaccacg tgtttctgga aagatcatcg catcaatatt atcgatactc
     5281 ccggtcacgt cgacttcacc attgaagtcg agcgttctct gcgtgttctc gacggtgcca
     5341 tcgccgtgtt ctgctcggtc gg
//
LOCUS       DASUEX010000389         9017 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1200674, whole genome shotgun sequence.
ACCESSION   DASUEX010000389 DASUEX010000000
VERSION     DASUEX010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9017
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1200674"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      209..275
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            508..882
                     /gene="crcB"
                     /locus_tag="VFV61_06515"
     CDS             508..882
                     /gene="crcB"
                     /locus_tag="VFV61_06515"
                     /inference="COORDINATES: protein motif:HMM:NF014584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEX4900169.1"
                     /translation="MQRIIFVGVAGLVGTLGRYWLSGLIARRYGETFPWGTLVVNALG
                     CFVTGAVYYVSEERFLVSPTVRSVILIGLLGGFTTFSSYGLQTFTLLREGEFGLAVMN
                     VTLSNVLGVLLIWAGYTLMKAL"
     gene            891..1226
                     /locus_tag="VFV61_06520"
     CDS             891..1226
                     /locus_tag="VFV61_06520"
                     /inference="COORDINATES: protein motif:HMM:NF014677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HEX4900170.1"
                     /translation="MNTLEEGYLLRIFIGESDRHQHHPLYEQIVLKARAAGLAGATVY
                     RGVMGFGKNSTVHTAKILRLSEDLPMVVEIVDSLEKVEKFLPVLDELIADGLVTLEKV
                     KVIHYQARE"
     gene            1346..3100
                     /locus_tag="VFV61_06525"
     CDS             1346..3100
                     /locus_tag="VFV61_06525"
                     /inference="COORDINATES: protein motif:HMM:NF028240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative porin"
                     /protein_id="HEX4900171.1"
                     /translation="MLITLRCSLFLFTGALAICFLATPKVSCQVAPGQPSPAAKAMLQ
                     FKSEPVGEKGTDEAARLSALEEAVREQHTQLEALQKLLVEQQETIKLLTMRLAAPGNA
                     NPELLVTTSPTANAKNSESAVAAQVPSVEDRLKKVEGRISEIGAIKFSGDVRLRSESF
                     FGLSNNLANGNNPVALGNELTPRHRMRVRARLALRGSVGDEFDWGLRFASGSFADNIS
                     TNQTLTDFYNRKPFGLDQAFITYKPKAVAGLRLQGGKFEPPWSSTELTIDSDLMVEGF
                     NESYTRNFKGSKFKELGFVAWQLPFLERNSAFVRNADGTVNIERSRRDGRDLALYGGQ
                     IRARVEPNDKVTLTLSAADLYFSGTQFISPVQVFGSQLQLPVTITIPATATTPAQTIT
                     TQVSIPRDFLVAGNGNLGLTNASNNATNRDGRLASGFNLVDLLAKLELKHSKRWPVTV
                     LLDFVTNTRVRDVVTAGPGGADLILTNHEKHAFWGEVLVGSTRAKGDMQLGYTFMRIE
                     KDAVLTPFNMSDITQQSDMRGHRLQFSYAADPRVTFSVIGIVTQRPNGVLGAFGTTPP
                     GSLNRATTRLQFDTVIKF"
     gene            complement(3475..3882)
                     /locus_tag="VFV61_06530"
     CDS             complement(3475..3882)
                     /locus_tag="VFV61_06530"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HEX4900172.1"
                     /translation="MGSAKKKVLFVCVENSNRSQMAEAFARIHGADEVEAFSAGSRPS
                     GTINPRAVKSMLELGYDLNTHTSKSLLDIPDIEYDFVATMGCGDECPFIRAKRREDWE
                     IQDPKHMEADAFRVVRDEIGARVKRVMRDLATE"
     gene            3990..5087
                     /gene="arsB"
                     /locus_tag="VFV61_06535"
     CDS             3990..5087
                     /gene="arsB"
                     /locus_tag="VFV61_06535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HEX4900173.1"
                     /translation="MQAQNAASLEGCSPLDRKRLSFLDRFLTLWIFAAMALGVLIGYT
                     NPEVEAFVNRFQSGTTNIPIALGLILMMYPPLAKVRYENLGEVFRDWRILGLSLLQNW
                     IIGPVLMFALAIIFLHDRPEYMVGLIMIGLARCIAMVIVWNELASGDNEYAAGLVAFN
                     SVFQVLFYSVYAWLFLSVLPPLFGLRGSIVNISIAQIAQSVFIYLGIPFIAGMLTRFL
                     LVKAKSKEWYHGVFIPRISPLTLIALLFTIVVMFSLKGNLIVQLPFDVIRIAVPLLIY
                     FVVMFLVSFYMGKRVGANYEKTATLSFTAASNNFELAIAVAVAVFGINSGAAFAAVIG
                     PLVEVPVLIGLVSVALWFQRRYFVSAVQPSH"
     gene            5216..5713
                     /locus_tag="VFV61_06540"
     CDS             5216..5713
                     /locus_tag="VFV61_06540"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX4900174.1"
                     /translation="MKSLLLMRHAKASQSTGGVSDSDRPLLAEGKDAAARVGAFIKRE
                     QFTVDFALSSPALRARETIESVLQAAGMSLQVHADPRLYEGGPLRLLEVLAELDNNLK
                     AVLVVGHNPVLEEFVHLLTEESVHLSPGTLAHLDLAATEWSEVSRVKHKLRRVVRPKE
                     LSKDD"
     gene            complement(5802..5990)
                     /locus_tag="VFV61_06545"
     CDS             complement(5802..5990)
                     /locus_tag="VFV61_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900175.1"
                     /translation="MRKKPAVKENEFQVVCIWCGKKIRTDKHEDTSGECLQCFYRILS
                     EKLLSQPRTFAGEFVSER"
     gene            6195..7280
                     /gene="pstS"
                     /locus_tag="VFV61_06550"
     CDS             6195..7280
                     /gene="pstS"
                     /locus_tag="VFV61_06550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEX4900176.1"
                     /translation="MLNALRTPRLISLLALSGLLLLAPACSPGGGQPGAAGSTISLQG
                     AGATFPNPLYQKWLSEYGKLHPNVRIDYQSIGSGGGIKQIKEQTVDFGASDSPMKDED
                     LKSAPRELVHIPTVLGAVVLTYNLQGIDKPLRFSPDVAADIFLGKIKQWNDPRIKTDN
                     PEAKLPAAEITVVHRSDGSGTSAVFTDYLSKVSSEWKEKVGAGTSPKWPVGLGGKGNE
                     GVTGQVKQTPNTIGYVELAYAIQNKLPVALLKNRAGDFVEPSFDAVSAAAAESLPITP
                     DDLRVSITDAAGKGTYPISSYTYILVYKDQRDAAKGKALIDFLWWGIHDGEQFARDLN
                     YAPLPAEIVKRAEAKINSIVSGGKPLR"
     gene            7335..8339
                     /gene="pstC"
                     /locus_tag="VFV61_06555"
     CDS             7335..8339
                     /gene="pstC"
                     /locus_tag="VFV61_06555"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEX4900177.1"
                     /translation="MNRNGAVQTDERKVAGLWSRWRLALSPTGNAGDAVFRGLLFTAA
                     FLVLLLVLAMIFALAVEARLSLRQFGFGFITGTDWNPVKGQFGALPFIYGTVVSSLIA
                     ILISVPLSLGIAIFLVEQAPKYLSRPLGFLVELLAAIPSVVYGLWGIFILAPFLRVHL
                     GPPLEHWLGWLPLFRGPVTGIGLLTGGMILAIMVTPIVSAIVRDVLTAVPSSQREAAL
                     ALGATKWETTRVVLVNGAPGIAGAVILGLGRALGETMAVTMVIGNRPEISLSLFAPSY
                     TIASVIANEFTEATEEIYLSALVELGLILFLVTFVVNAIARVLVWNVTRKTGGVGGAA
                     "
     gene            8377..>9017
                     /gene="pstA"
                     /locus_tag="VFV61_06560"
     CDS             8377..>9017
                     /gene="pstA"
                     /locus_tag="VFV61_06560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEX4900178.1"
                     /translation="MKRTDKIRRSTSAMMTTLTAICTIAAVSILLIILGYIALQGISS
                     LSFNFLIDSPKPVGEGGGIGNAILGSLVLLLLASVIGLPLGIAIGIYLSEFGAGKFGS
                     LVRFMVDTLTGIPSIVTGVFVYTIVVLPLRHFSALAGGIALAMIMIPIVARTTEEMIR
                     LVPHSLREGALALGAPQWRVTLGVVVPAAASGIATGAMLAIARISGETAPLLFT"
BASE COUNT         1947 a         2375 c         2467 g         2228 t
ORIGIN      
        1 cagaaagtac gtccaggcct tccagcctga catgggccga gtggaacgtc aacccgggcg
       61 gggacgcggg gacacgggga cgcggggaca cggggacacg gggacaccgg aatcttttag
      121 caagtcagca atgctcaacc atccggcaat acgctcttaa aaactttgcg tctctgtatc
      181 tttgcgactc tgcgattagc ttaaggctac aagaaacggc gatggggttc gccataacca
      241 tcccgcggag cgggattgat gactcctaat tagtagcgtg atagcgcgcg aacggttagg
      301 agctttttca tattgccaat tggcaattgc caattgccga ttggaagata ccgacgaagc
      361 caattttgaa atcagccaag cggcaatgta acaggctcac cggctttccc gacatgtcgg
      421 gatactgact gcttacgctg cgagccaatc ggcactcggc aatcgccact cggcaatgag
      481 ttgagtggct gactcaaaac ttcaattatg caaagaataa tttttgtagg cgtcgctggg
      541 ctcgtgggta cgctcggcag atattggctc tccggcttga tcgccaggcg ttacggggaa
      601 acctttccct ggggaacgct ggtggtaaac gccttaggtt gttttgttac gggtgcggtc
      661 tattacgtgt cggaggagcg atttctagtg agccccaccg tgcggagcgt aatcctgatc
      721 ggactcctgg gcgggttcac tacattttcc tcctatggac tgcaaacgtt tactctcctg
      781 agggaaggtg aatttggtct ggccgtaatg aacgtcacgc tttccaatgt gctcggggtg
      841 ttattgatct gggccggtta cacattgatg aaagcactgt gaggctgcgt atgaatacat
      901 tagaagaggg ctacttgctg cggatcttca ttggcgaaag cgatcggcac caacatcatc
      961 cactttacga gcaaattgtt cttaaggccc gtgcggctgg attggcgggg gcgacggtct
     1021 atcgcggcgt aatgggcttc ggtaagaaca gcaccgtaca cactgcgaag atcctccgac
     1081 tctcggaaga tttgccgatg gtggtggaga ttgttgattc gctggagaaa gtcgagaagt
     1141 tcttgccagt acttgatgag ctgatcgctg atggattggt cacgctcgaa aaggtcaagg
     1201 ttattcatta ccaggctaga gagtaacggt cgcggtccac ttcccttcaa tcagaaccct
     1261 tcacatagat ttaacatcgc gttaagtttg aattcaccca acgcgaatag tctgactttc
     1321 tcgattcatc ctgcgggagg acaacatgct aatcactctg aggtgcagtc ttttcctctt
     1381 caccggcgcg ctcgcgattt gttttctggc aacaccgaag gtttcttgtc aggtcgcacc
     1441 aggacagccc tctcctgctg ccaaggcgat gttgcaattc aaatctgaac ccgtcggcga
     1501 gaaagggacg gacgaggctg cccggctaag cgcgctcgaa gaagcggtac gcgaacagca
     1561 cacacagttg gaagccttgc aaaaactgct ggttgagcaa caggagacta taaaacttct
     1621 gactatgcga ctggcggctc cgggcaacgc taatccagag ctattggtaa caacgtcgcc
     1681 tacagcaaac gcaaagaatt cggaaagtgc agttgccgcg caagttcctt cagttgagga
     1741 tcgcctaaag aaggttgaag gacgcatctc agaaatcgga gcgatcaaat tcagcggcga
     1801 cgtgcggctg cgatccgaat ctttttttgg cctctcaaat aatctcgcca atggcaacaa
     1861 cccagtagca ctgggaaacg aactcacccc gcgacatcgc atgcgggtgc gtgcgcgcct
     1921 cgcccttcgc ggttctgttg gggatgaatt cgattgggga ctgcggttcg cgtcgggcag
     1981 cttcgccgac aatatctcca ccaaccaaac gctaaccgac ttttataacc gtaagccctt
     2041 tggcctggat caggctttca ttacctacaa gccaaaagcc gtggctggct tgcgcttaca
     2101 aggcggtaag tttgaaccgc cgtggagctc tacggagctg accatcgaca gcgatctaat
     2161 ggttgaaggg ttcaatgagt cgtacacgcg aaacttcaaa ggttcaaagt tcaaagagct
     2221 tggctttgtt gcctggcaat tgccctttct ggagcgcaac tcagcttttg tgcgcaatgc
     2281 cgacggcact gtcaacatcg agcgttcgcg ccgcgatgga cgtgacctgg cgctttacgg
     2341 cggccaaata cgcgcccgcg tagaacccaa tgacaaagtc acactgactc tctcggcagc
     2401 tgatctttac ttctcgggca cgcaattcat ttcgccggtt caggtctttg ggagccagtt
     2461 gcagttgccg gtaacaatca ctattccggc cacagccacc acgccggcgc agacgattac
     2521 cacccaggtt tctattccgc gggacttcct tgtggccggc aacggcaatc ttggactcac
     2581 caacgctagc aacaacgcta ccaaccgcga cgggcgtctc gcttcaggtt tcaatcttgt
     2641 cgacttgctt gcaaagctcg agttgaaaca cagcaagcgt tggccggtta ccgtcttgct
     2701 cgactttgta accaacacgc gggtgcgcga tgtggtgacg gcgggtccgg gaggggctga
     2761 tttgatctta actaatcacg aaaagcacgc cttttgggga gaagtattgg tgggttcaac
     2821 acgagcgaag ggtgatatgc agcttggtta caccttcatg cgcattgaga aagatgcagt
     2881 gcttacccca ttcaatatgt ctgacataac tcagcaatca gacatgcgcg ggcatcgttt
     2941 gcagttttcc tatgccgccg atccacgtgt gactttttcc gtgattggta ttgtgacgca
     3001 gcggcccaac ggcgtgctgg gtgcttttgg cacgacgccg cctggctctt tgaaccgagc
     3061 gaccacgcgc ttgcaattcg acactgtgat caagttctga agggttgggt ctctaggcga
     3121 aaaccagaag agcaatgtca tcgtgtttgg gttccaagag cgccgcaggc gcggctttca
     3181 atagccgcgg ccggaaggcc gtggatccaa gacctgaaga tgaaatcgag gcgcgcagga
     3241 ccggcattcc tctgcactgt gaaatgggtt tattgccgca ccttcggcgc tcgactattt
     3301 aggtgtcgac tgaccacggc ctcacggccg tggctatttg atgacggtcc tgcggacctc
     3361 acttggcact ctcagacgtg tgtctgattc cgctgaaatt gagtcaagta tattatcgcg
     3421 gtttacaccg ggctgaagcc ccagtgtgaa tgagaaattc agactcatta aagttcattc
     3481 tgtggccaaa tctcgcatga cccttttgac tcttgcccct atctcatcgc gcacaacacg
     3541 aaacgcgtct gcttccatgt gcttgggatc ctggatttcc caatcctctc gccgcttcgc
     3601 gcggataaag gggcactcgt cgccgcagcc catcgtcgcc acgaagtcgt actcaatgtc
     3661 ggggatgtcc aacagagact ttgacgtgtg cgtgttgaga tcgtaaccca gctcaagcat
     3721 tgacttcaca gctctgggat tgattgttcc tgaaggacgc gaaccggcgc tgaaggcctc
     3781 tacttcatca gcaccgtgaa ttcgcgcgaa cgcttcagcc atctgactgc ggttggagtt
     3841 ttctacgcag acgaaaagca ctttcttttt tgctgaaccc atagtcctct ttcacccgaa
     3901 tgtatttctc acgtcaaccc attctgctca ccgtgctaga gtgtcagccc cgggcgcgga
     3961 gttcactcgg attctaactt ataaaactta tgcaagctca aaatgctgcc agtttagaag
     4021 gttgttcgcc gctcgatcgt aagcgccttt cattccttga ccgcttcctc actctctgga
     4081 tctttgcggc gatggcgtta ggagtcctga tcggttacac gaacccggaa gtcgaggcct
     4141 tcgttaatcg cttccaatcc ggcacgacca acattccgat cgcgttggga ctgattctga
     4201 tgatgtaccc gcccctcgcc aaggtgcgct acgaaaacct gggagaggtc ttccgcgatt
     4261 ggcgcatact tgggttatcg ctcttgcaaa actggatcat cggccccgtc ttgatgtttg
     4321 ccctcgctat cattttcctc cacgatcgac cggagtacat ggtcggcttg ataatgattg
     4381 gactggcgcg ttgtattgcg atggtcatcg tctggaatga acttgcgagt ggcgacaatg
     4441 aatacgcagc cggtctcgtc gccttcaaca gcgtatttca agtgctgttt tatagtgtct
     4501 atgcatggct gttcttatca gttctgccac cccttttcgg actgcgtggc agtatcgtca
     4561 acatcagtat cgcgcagatt gcgcagagtg ttttcatcta cctggggatt ccattcatcg
     4621 ctgggatgct gacgcgcttc ctcctggtca aagccaaaag caaggagtgg tatcacggcg
     4681 tcttcattcc caggatcagc ccgcttacgc tgatcgcgct cttgtttacc atcgtcgtaa
     4741 tgttttcgct caagggaaat ttgattgtgc agcttccctt cgacgtcatc cgcattgccg
     4801 tgccgctgct gatttacttc gtcgtaatgt ttctggtgag tttctacatg ggcaagcgag
     4861 tcggcgcaaa ctacgaaaaa accgcgaccc tatccttcac ggcggcgagc aataatttcg
     4921 agttggcgat agcggtggcg gtcgcggtct ttggtatcaa ctcgggcgcc gcgtttgcag
     4981 cggtgattgg gccgctggtt gaagtgccgg tattgatcgg attagtgagt gtcgcgctgt
     5041 ggtttcagcg ccgctacttc gtgagcgccg tacaaccatc gcattgacga aattggcaat
     5101 gtcgatggcc gctaccgcgt tgcggcactg acttaattcc gcttcgagcc aattggcaat
     5161 cggcaatcgc caatctgcaa tcggcaattt aaggcatgac tgtgagatca cgaaaatgaa
     5221 gagtttgctt ctgatgcgtc acgcgaaagc gagtcagagt accggcggcg tttctgactc
     5281 cgaccggccc ttgctcgctg agggcaaaga cgcggcagcc cgtgtgggcg ccttcatcaa
     5341 gcgcgagcag ttcacagtcg atttcgcgct tagttcaccc gcgctcaggg ctcgagagac
     5401 gattgagtcg gttttgcagg cagctggaat gtctctgcaa gttcacgctg acccgcgact
     5461 gtacgagggc ggacctttgc gactcttgga agtgctcgct gaactagata acaatcttaa
     5521 agcagtactt gttgtcggac ataatccggt gctcgaggaa ttcgtacacc tcttgactga
     5581 agagagcgtg catctttcac caggaacact tgcccacctc gatcttgctg ccacggaatg
     5641 gagtgaggtt agtagagtga agcacaagct tcgcagagtc gtaaggccta aagaattatc
     5701 gaaagatgat tgaattgccc gattgccact ggtcccattt cagttgtgct tatcggtaaa
     5761 ggccaagtgg aaatcgccaa tcggcattcg gcaatcgata ttcagcgttc gctgacaaac
     5821 tcacccgcga acgtacgtgg ctgcgagagc agcttctcac tcagtattcg atagaagcac
     5881 tgcaggcatt caccgcttgt atcttcgtgc ttgtcagttc ggatcttctt tccacaccag
     5941 atgcacacaa cctgaaactc attctctttc actgctggtt tcttcctcat tcacaaacct
     6001 cccaacttgt ttccgctgat cataactctc gatagttacg gtcccatggc gcgaaagtta
     6061 aatcgcggtt aagaaattct taccgactct gaaggcggcg attgtttacc cagagttcag
     6121 atgaagttca gatagatgta acaccccaca actaagctct cccttggcca cccaaaattc
     6181 aggagaaaat ttctatgttg aatgccttga ggactccccg cctgatttcc ctgcttgcgc
     6241 tttccggtct gctattgctc gcccctgctt gtagtccggg cggcggacag ccgggcgctg
     6301 ctggatcgac aattagttta cagggggcgg gtgcgacttt tcccaacccg ctttatcaaa
     6361 agtggctgag cgagtatggc aagctgcatc ctaacgtgcg cattgactat caatcgatcg
     6421 ggtcgggtgg cggaataaag cagatcaagg aacagacggt tgactttggc gcttccgact
     6481 cgccgatgaa ggacgaggat ttgaagagcg cgccgaggga actcgttcac attcctactg
     6541 tgctcggtgc agtggtcctt acgtacaacc tgcaaggcat cgacaagccg ttgcgatttt
     6601 ctcctgatgt ggccgctgac atttttctcg gaaagataaa acagtggaac gatccccgaa
     6661 tcaaaactga caatcctgag gcgaagcttc cggctgccga aatcaccgtg gtgcatcgct
     6721 cggacggcag cggtacgtcc gcagtgttta ccgattactt atccaaagtg agttcggagt
     6781 ggaaagaaaa agtcggcgcc ggaacttccc cgaaatggcc cgttggtctg ggcggcaagg
     6841 gaaatgaagg cgtgaccggc caggtgaagc aaacccccaa tacgatcggt tatgtggaac
     6901 tcgcttacgc gattcagaac aagttgcccg tggccctcct caaaaacaga gccggtgatt
     6961 ttgttgaacc aagcttcgac gcggttagtg ctgccgcggc cgaatcgttg ccgataacgc
     7021 ctgacgacct gcgcgtgtcg atcaccgacg ccgcgggcaa aggtacttac cccatttcca
     7081 gctataccta catccttgtc tacaaagacc aaagagatgc cgcgaaggga aaggcgctga
     7141 tcgattttct ctggtggggc attcacgacg gtgagcaatt tgcccgggac ctcaactatg
     7201 ccccgctgcc ggcggaaatt gtgaaacgtg ccgaggccaa gatcaattcc atcgtttctg
     7261 gcggcaaacc gctgaggtag tcgagttcgg catttgacga cgcatcagtc gtcagcccgt
     7321 gcttctcatt tccaatgaac cgcaacggcg cagtgcagac cgacgaacga aaggtggccg
     7381 gactctggtc aaggtggcgc ctggcgttgt cgcccactgg caacgccggc gacgcagtgt
     7441 ttcgtggttt gcttttcact gccgcgttcc tggtgttgtt gcttgtcctc gcgatgattt
     7501 ttgctctcgc tgttgaagcg agactgtcac tgcgacagtt tggctttggc tttatcacag
     7561 gaacagattg gaatcccgtt aagggacagt ttggagcgtt gccattcatt tatggcactg
     7621 tggtctcctc attaatcgcg attctaatca gcgtgccgct ctcgctgggc atcgcgatct
     7681 ttctcgttga gcaggcgccc aaatatctct cgcggccgct gggattcctg gttgaattgc
     7741 tggcagcaat tccttcagtg gtctacggcc tctggggaat tttcatcctg gcgccattcc
     7801 tgcgtgttca tctggggcca cctctggaac attggctcgg ttggttgccc ctgtttcgtg
     7861 ggccggttac tggaatcggc ttactaaccg gcggcatgat cctggcaatc atggtgacgc
     7921 cgatcgtctc ggcgattgtg cgcgacgtgt taaccgccgt gcccagttct caaagggaag
     7981 cggcgttagc cctgggtgca accaagtggg aaacgacgcg agtagtccta gttaatgggg
     8041 cgccgggaat tgccggagca gtcattctgg gattaggccg cgcgctgggc gagacgatgg
     8101 cggtcacgat ggtaatcggc aatcgccctg aaatcagtct gtccttgttt gctccgtcgt
     8161 acacgattgc ctccgtcatc gcgaacgaat tcactgaagc aacggaagag atctacctca
     8221 gcgctttggt tgaactgggg ctgatccttt ttctggttac gttcgtcgtt aatgcgattg
     8281 cacgagtgtt ggtttggaac gtaacgagaa agacgggtgg tgttggtggg gcggcgtaac
     8341 gcaaactgat agcttacgtt tgcagtacag aatgagatga agcgtacaga taagattcgg
     8401 cgcagtacca gcgcaatgat gacgacgcta acagcaatct gcacgattgc ggccgtcagc
     8461 attctgttaa tcattctcgg ctacattgcg ctgcagggaa tctcgtcgct cagttttaac
     8521 ttcttaattg atagtccgaa gcccgttgga gaaggtggtg ggatcggaaa cgcgattctc
     8581 ggatctcttg tgctgctgct gctcgcatcc gttatcggtc ttcctctggg catcgcgatc
     8641 gggatctatt tatccgagtt tggcgccggg aagtttggca gcctcgttcg ttttatggtc
     8701 gacacgctga ctggcattcc ttcgatcgta accggtgtct tcgtctatac gattgtagtt
     8761 ttaccgctaa gacacttctc tgcgctggct ggaggaattg cgctcgcaat gatcatgatt
     8821 ccgatcgtcg cgcgcactac tgaggaaatg attcgtctgg tgccccatag cttgcgcgaa
     8881 ggcgccttgg cgctcggtgc gccgcaatgg cgagtgacgt tgggagttgt ggtgccggcc
     8941 gcagcttctg gcattgcgac gggagcgatg ctggcaatcg ccagaatttc cggggagacg
     9001 gcgccgttgt tgtttac
//
LOCUS       DASUEX010000390         2361 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_291710, whole genome shotgun sequence.
ACCESSION   DASUEX010000390 DASUEX010000000
VERSION     DASUEX010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291710"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="VFV61_06565"
     CDS             complement(<1..509)
                     /locus_tag="VFV61_06565"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF019887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX4900179.1"
                     /translation="MPIRLLALDLDGTLLNSRGQVSERNRSALEAARSCGVRVAIVTG
                     RRFRDARPIALELGLDVPVIAHNGALTKHAETLETVAVLPLPLAAAHEAIKIGRSAGA
                     DPLVSDDHEGLGVMIYDHLSGDNKPLLQYIAWAQRIHGETSEASVRKVEAVEEYLDHE
                     PVHLAFSGT"
     gene            804..1967
                     /locus_tag="VFV61_06570"
     CDS             804..1967
                     /locus_tag="VFV61_06570"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF006050.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEX4900180.1"
                     /translation="MNITQLECAACGLRHEAQRLLNLCRECGKPLLVRYDLEQAGRSL
                     KKESLPGRRPDLWRYREVLPVANEANIVSLGEGWTPLLSAERLGQKLGLNTLFIKDES
                     QNPTQSFKARGMTAAVSMAKELGVKKLAVPSAGNAAGALAAYAARAGLECFIFMPRDT
                     PRANVVECEQTGAHVTLLDGLITDCGAEVARRKDTEGWFDVSTLKEPYRVEGKKTLGY
                     ELAEQGDWSLPDVIIYPTGGGTGLIGMWKAFDEMEQLGWIGSQRPRMVSVQASGCAPI
                     VQAFTEGKRFADEFPNAATVASGLRVPRAIGDFMILDAIRSSGGTAIAVTDEELLAAV
                     AEIGALEGVFCAPEGAACLPALRKLLADKAVTPDETVVLFNTGAGVKYLESFS"
     gene            2047..>2361
                     /locus_tag="VFV61_06575"
     CDS             2047..>2361
                     /locus_tag="VFV61_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900181.1"
                     /translation="MKYLFLFVLAAILLNSALVLGQEKNKRFESTRIEQLRGSGLEAL
                     YNLDYGKAQNDFSEIVKLDPRNPTGPQLLAARLWIKTLYESRRLQSSLYSSESFYTKG
                     DDK"
BASE COUNT          545 a          636 c          676 g          504 t
ORIGIN      
        1 taggtgccgg agaaagcaag atggacgggc tcatgatcca aatattcttc gacagcctcg
       61 accttgcgaa ccgatgcctc tgaagtctca ccgtggatac gctgcgccca ggcgatgtat
      121 tgcagcaagg gtttattatc gccgctcaga tgatcgtaga tcatcacgcc taacccttca
      181 tgatcgtcgc tgacaagggg atcggcgccg gcgctgcggc cgatcttaat cgcttcgtgc
      241 gcggcggcga gcggcaaagg aagaacagcg acggtttcca gagtctcggc atgttttgtc
      301 aacgcgccgt tgtgcgcgat tacaggcacg tcaagcccca gctcgagggc gatgggccgc
      361 gcatcgcgaa agcgccggcc ggttacaatc gcgacccgta ctccacaact gcgcgccgct
      421 tcaagggcgc tgcgattgcg ctcagatacc tggccgcgcg aattgagcag ggtgccgtca
      481 agatcaagtg caagcaaacg aatgggcatt aaggttaagc ttaatagaag ataattgcga
      541 agtgacaaga tagagtgttg gtagtccaaa gtccaaagtc caaagtccaa tgtccaaagt
      601 ccaatgtcca aagtccagag tcctgctgag ccggcctttc attttctcaa atggagcgtt
      661 gcctgcctct agcttaaatg gcggactttc gacattggac attggactcc aaatgggctg
      721 ccgtatctat ccgacgtctg acgccgtaat attcatcctg ctgcccagtc aaagcacatt
      781 tattctcgga gactcgctaa atcatgaaca ttactcagct tgaatgtgcc gcgtgcggcc
      841 ttcgccacga ggcacaacgg ttgctcaacc tctgtcgcga gtgcggcaag ccactgctgg
      901 ttcgctacga cctggaacaa gcaggacgct cattgaaaaa ggaatcactg ccgggacgcc
      961 ggccggacct ctggcgttac cgcgaagtct tgcccgtcgc aaacgaggcc aacattgttt
     1021 cgctgggcga aggttggacg cccttgctca gcgccgaacg cctgggacag aagctcggtc
     1081 tcaacacgct gttcatcaag gatgagtcgc agaatccgac gcagagcttt aaagcccgcg
     1141 ggatgactgc cgcggtctcg atggcgaaag aactcggcgt taaaaaactt gccgtccctt
     1201 cagcgggaaa tgccgcaggg gcacttgcgg cgtatgcggc ccgagccgga ctggagtgtt
     1261 tcatcttcat gccgcgcgat acaccgcggg caaacgtagt cgagtgtgaa caaaccgggg
     1321 cgcacgtaac cttgctcgac ggtttaataa ccgactgtgg cgccgaagtc gcacgtcgca
     1381 aagatactga gggctggttc gatgtttcga cgctaaagga accgtatcgc gttgaaggaa
     1441 agaaaacgct tgggtatgaa ctggctgaac aaggcgattg gagtctccct gacgtcatca
     1501 tctatccgac tggtggcggc accggattaa tcggcatgtg gaaagccttt gacgaaatgg
     1561 agcaactcgg ttggattggt tcgcagaggc cgcgcatggt ttcggttcaa gcttcgggat
     1621 gcgcgcccat cgtccaggcg ttcacggaag ggaaacgctt tgcggatgag tttcctaatg
     1681 cagcaacggt cgcctcaggc ctgcgcgtgc cgcgcgcgat cggcgacttc atgatactcg
     1741 acgccatccg gagctccggg ggtacggcga tcgccgtcac tgatgaagaa ctgctcgcgg
     1801 ccgtcgccga gattggcgct ctcgaaggag ttttctgcgc gcccgaaggc gctgcctgtt
     1861 tgccggcgct aagaaagttg cttgctgata aagcagtgac tcctgatgaa actgtcgtgc
     1921 tcttcaatac cggcgcgggg gtgaagtacc tggaaagttt ctcttagccc agttcagaga
     1981 taactacttc aagcgcttcc gttccggatg gatttacaac ttggtcttga aaggaagaag
     2041 gaagcgatga agtatttgtt cttatttgtg ctcgcggcga tcttgctcaa ttcggcactc
     2101 gttttgggtc aggaaaagaa taagcggttc gagtcgacca ggatcgagca attgcgtggc
     2161 agcggtctgg aagcacttta caatctggac tacggcaaag cgcagaacga cttcagtgaa
     2221 atcgtaaaac tagatcctcg caatcctacc ggaccgcaac tgctcgccgc gcggctctgg
     2281 atcaagacac tttacgaatc gcggcggcta caatcctccc tctacagttc cgaatcgttc
     2341 tacaccaagg gtgacgataa a
//
LOCUS       DASUEX010000391         3133 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1444959, whole genome shotgun sequence.
ACCESSION   DASUEX010000391 DASUEX010000000
VERSION     DASUEX010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3133
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1444959"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="VFV61_06580"
     CDS             <1..190
                     /locus_tag="VFV61_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900182.1"
                     /translation="HRTSENLFKKGVGLANTETRLERLYGTDHLFDFSNNPTGGLVVT
                     LEIPFHKDNVTPAPSETA"
     gene            316..1095
                     /locus_tag="VFV61_06585"
     CDS             316..1095
                     /locus_tag="VFV61_06585"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEX4900183.1"
                     /translation="MTDPTLIRVLLVDDEPLALSMMREMLQGDPQVEIVGESVNGREA
                     LEAIQSLTPDLLFLDVQMPELGGFEVLANLEEGQIPYVIFVTAYDQYAVRAFEVHALD
                     YLLKPFDQERFNSSWQRARARILSERNGGMDQRILTLLEDLKAGSKYLERLVIKAGGR
                     IYFLDTNEIEWIEAEGNYVSVHTAKKSHLIRETIGSLESQLDPKKFVRIHRSSIVRIA
                     SIRELEPWFHGEYRVNLENGTQLALSRNYRDKLQEALGKLP"
     gene            1336..2094
                     /locus_tag="VFV61_06590"
     CDS             1336..2094
                     /locus_tag="VFV61_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900184.1"
                     /translation="MRLRDCLIAATLMSLTLITTLARPLPPRTECADGSLKPSAPARM
                     VVPPAPALIAGDEVLEGVYYNTLAILSSSNRCSDFFGGPSASMEVFSQLMRQVRKDVL
                     APSIAMRMHGETINAEDARSNLRYRLFAKVSINTNGPFYRKTNFRSERTIFGVGSFAP
                     NTREVRVLILLHELGHLMKGPDGNWLLPDDGGKEELSRDNSRKIEKVCGDQISALGNG
                     EALRNLALRIQPEERLALDRNNSEPSPLDRTGNH"
BASE COUNT          781 a          843 c          828 g          681 t
ORIGIN      
        1 acatcggaca tccgagaatc tgttcaagaa gggtgtggga ctggcaaata cggagactcg
       61 gttggagcga ctatacggaa cggatcatct cttcgacttc agcaacaatc ccacgggtgg
      121 gttagtcgtt actttggaga ttccgttcca caaggacaac gttactcccg ccccgtcaga
      181 gactgcttag tctccagccc ttcgatcaat tccaggattt aaaagttttt gccagataac
      241 tccccgtcgt ggtgtaagta caacaaggac tcccctgacc tcgtaactcc gaatcgttcg
      301 cagaaagcaa gatccatgac cgaccccaca ctcatacgag tgctgttggt ggatgatgag
      361 cctcttgcgc tctcgatgat gcgtgaaatg ttgcaaggcg acccgcaggt agaaatcgtc
      421 ggagaatccg taaacggccg cgaagcgctg gaggcaattc agtcgctcac tcctgacctg
      481 ctcttcctcg atgtccagat gcctgagctg ggcggctttg aagttcttgc caatctggaa
      541 gagggacaaa tcccttatgt catctttgtc accgcttacg accagtacgc cgtccgcgcc
      601 tttgaggtcc acgcactgga ctacctgctg aaaccgtttg accaggaacg tttcaatagc
      661 tcctggcagc gcgcccgggc ccgcattctg agtgaacgaa atggcggtat ggatcaacga
      721 atcttgacgc tactggaaga cttaaaggcc ggcagcaagt acctcgagag gctcgtaatc
      781 aaggccggcg ggcgtattta cttcctggac acaaatgaga tcgaatggat cgaagccgaa
      841 ggcaattatg tgtcagttca taccgcaaaa aaatcccacc tgattcgtga aactatcggc
      901 agtctcgaat cgcagctaga cccgaagaaa tttgtgcgca tccaccgctc ttccatcgtg
      961 cgcatcgcga gcatcaggga attggaaccc tggttccatg gtgaatatcg cgtcaacctg
     1021 gagaatggaa ctcagctcgc gctctctcgc aactatcgag acaagctgca agaggccctg
     1081 ggcaagctac cttgagagac gagttgtcac tgtctcaagc ccgttagtca caagagcctg
     1141 ccttcccgtc ataaaaacga agcgaacctc gcaactcaca cgtataaggg tcaagtaatt
     1201 tctgggtgat ttaagagtcg cgttaaccca gcgcacttct agaaagcctt taccgcgttg
     1261 ctcatgctca gctcggtccc caacgagcgc aagtacggac gaaactccta atgactttgg
     1321 gcaggaggaa ctcccatgag actacgcgac tgcttaatag ctgcaacact gatgtcctta
     1381 acattgatta caaccctggc caggcccctg ccaccgcgaa ccgagtgcgc agacggttca
     1441 ctcaaaccca gcgctccggc gcggatggta gtgccaccgg cgccggcatt gatcgctggg
     1501 gatgaggtac tggaaggggt ctactacaac acactcgcta ttctgagttc gagcaacaga
     1561 tgtagcgact tctttggtgg ccccagtgcc tcgatggaag tgttcagcca gctaatgcga
     1621 caggtgcgca aagacgtcct ggctccgtcg atcgccatgc gcatgcatgg ggaaacgatc
     1681 aatgcggagg atgccaggag caacttgcgc taccggctgt tcgccaaggt ttcaatcaat
     1741 accaacggtc cgttttatcg gaagacaaat tttcgttcgg aacgaaccat atttggtgtg
     1801 ggaagtttcg cgcccaacac gcgggaagtt cgcgtgctga ttcttttgca tgaactcggc
     1861 cacttgatga aagggccgga cgggaattgg ctgctgccgg atgacggcgg taaagaggaa
     1921 ctgagccgcg ataactcccg caagatcgaa aaagtctgtg gcgatcaaat tagcgccctc
     1981 ggaaatggcg aagccttaag gaacctcgcc ctgcgaattc aaccagagga aaggctcgcg
     2041 ttggatcgca acaattccga gccgtctccc cttgatcgca cagggaacca ctaatcaagg
     2101 gatacgcacc taaaggagaa gaacatgaaa gcgacagttt gggagagtga tggaatggag
     2161 ctcagcgcgg ctccgcaaaa cgggaacgaa gaatctgcaa ccgctcctca caacctcacg
     2221 actctgaagg agctaacgct ccgacttctt cgggaggtgc aatgcatcag cgaggttcgc
     2281 ccgtccagtt ttgaaaatgg gctcgacttc tatgaagaag tgactcggtt cgaggttgat
     2341 cttatcaagc gcgctttgtt gctgacagca gggcatcaag ggcgtgcggc aagattattg
     2401 aacttaagag ctacaacgct aaattcaaaa atcaaacact acggcatcag ccttgaaggg
     2461 tttgttagcg gttacccttt ccttgagaca agcgctaaca ccaggcgtca acacgtttga
     2521 gtttcggatc tgaataactc cgaaacatct cgcacccagt ctgccgcttt agaagcgggg
     2581 ctgggtgctc ctatttttgc tgcttgtgta gcgctctgtg ggccacttct gtgctccaac
     2641 gcgtagcgtt gtgaacactt tagtccggcg tttcaacgcc gggtttcagc cccgtattag
     2701 cccggcgtcg cgtcagcgac gcgtgaatcg ttgctaggcg acgaagccgc taacccggcg
     2761 ttgaaacgcc gggctaaagt caacgcgacg ctacgcgtcg atttccctct acgaaaaaaa
     2821 attgcaggtt tgaaagagtc acacctcatc cacatcagaa ctcaattcca aattctgcag
     2881 aatccaaaac tcgcaatctc aagttctggg gacggtgcgg cgggtaggcc gaggcaagtg
     2941 atgagacggc ctcctgagaa tgtagcttag acggcacgat cgttgcgctt tcacaaccgt
     3001 gccaactccc cgttgcatcg ttggccatcc gagtttctgg aatctcgagg taccactcgg
     3061 gggcggagga acagtcctaa cgatcaatga atttgaagct gccagatgat ttggtgtgac
     3121 acgcagacca gat
//
LOCUS       DASUEX010000392         6590 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1566120, whole genome shotgun sequence.
ACCESSION   DASUEX010000392 DASUEX010000000
VERSION     DASUEX010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6590
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1566120"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..1301)
                     /locus_tag="VFV61_06595"
     CDS             complement(15..1301)
                     /locus_tag="VFV61_06595"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX4900185.1"
                     /translation="MKTLTKAVIGLSAAGAAGAAYVWVEKRKHAAADEAQSRVDIHGF
                     VSNGYEAVREAFAENFSKRHEIGAACCIYHKGEKVVDLWGGVRNKATGEPWEENTMAL
                     VYSATKGLAAMTLAIAHSRGWLDYDALVCKYWPEFAQNGKERITVRQLLAHQAGLFAL
                     DEPVDRSTVADLDRLAEILARQKPAWEPGTRQAYHAITLGYYESELLRRIDPQHRSLG
                     QFFQEEIASPLGLDFYIRLPESIPDSRFATLVDPSFFEMLFGFPIRLTPSVWNHRSNI
                     RRALMGSEFPHDEKRIYSRNLEVPAGGGVGTARAIARAYSVFATGGKELGLREETLQE
                     LIAPAIPATHGFYDECLKGEAQFSLGFVKTDSGFPIGNPGSFGHPGAGGSLGFADPQT
                     QIGYGYIPNRKGVAIDGDPRDVALRQALYSATPAAG"
     gene            complement(1371..2561)
                     /locus_tag="VFV61_06600"
     CDS             complement(1371..2561)
                     /locus_tag="VFV61_06600"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX4900186.1"
                     /translation="MKRILNTIVLIIVAVAVVALVTMWLRPEPTKVDTAKTTRGSLRV
                     TVDGEGKTRVRDRYVVAAPVAGRIQRITLRRGDAVEKGQVVALIETLPLTPLDPRQRA
                     EAIARVNAAQDTKREVDRMIERSRAIYDQARRDCDRCEILARSGDISRQELEKAQTEV
                     ATSFREYEAARSRAESAAHEVEVASAALLAADQSPRAATAIVKVQAPVRGRVLRLVEE
                     SERVIAAGAPLVELSNPSMLEVVIELLSTDAVKVRPGALVLIEDWGGTESLEARVRLM
                     EPSAFTKVSALGIEEQRVNVVADLTRASTELGDGYRVEGRVVVWEAENVLQVPVSALF
                     RRGESWSLFVVENGEAHLREVEVGQRTPFAAEIKSGLAEDATVIVHPSNQISEGVRVE
                     AQEK"
     gene            complement(2579..4945)
                     /locus_tag="VFV61_06605"
     CDS             complement(2579..4945)
                     /locus_tag="VFV61_06605"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX4900187.1"
                     /translation="MATLNRKLIRDVIHLRGQVAAITLVVACGVASFVAMRSTYNALL
                     ASQQSYYERYRFAEVFAQLKRAPDSIAMRISEIPGVGAAEFRVVADVTLDVPGLDEPA
                     KGRIISIPEERRPMLNELHLQEGRYLESGRRDEVLVSGAFATANNLKPGSTLSAVINS
                     KWQKLQIVGIALSPEFVYEIKSGEMFPDHRRSAVLWMSRDALGPAFNMKGAFNDVALT
                     LAPGAVEADVLERVDELLASYGGFGAYNRNDQISHRFLSNEIAELQVTGTFIPAIFLA
                     VTAFLIHLVLSRMVATQRQEIAVLKAFGYGNSSIGFHYLKLAFVVVLSGVALGIAVGW
                     YFGFKITELYTEFFRFPVLRYQPGASVIFGAVVISLGSAAIGALAAVRRAVSLPPAEA
                     MRPEPPARFRSGFIERLGFHQLVPTSVRIILRNLERRPGKAMVSIFGIALAVGLLVVG
                     FFLYLDTIGRIIDVQFHQVQREDASVAFHEPRPASVKYDLAHLPGVIRVEPYRTVPAR
                     LRVGHRTRRTAVLGLESASDLRRIVTKDSQNVTLPADGLLLTTQLAEILGVKPGESVT
                     VEVLEGARPVRDVAVAGTVDELVGLSAYMNLRALNKLLGEGQSSSGAFLMVESARIPE
                     LYSELKQTPAVSAVSIPEVALASFNETIARTMNVSTVILIGFAVIIAFGMVYNGARIA
                     LSERGHELASLRVLGFSRGEIGFMLLGEQAILTALAIPLGFALGYGIAWLITVVIDTE
                     LIRFPLFISAKTYTLAFLVVAGSAFLSGYLVRWRLRHLDLVAVLKTRE"
     gene            complement(4966..5802)
                     /locus_tag="VFV61_06610"
     CDS             complement(4966..5802)
                     /locus_tag="VFV61_06610"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4900188.1"
                     /translation="MTYLSQSAVTTPEDARACLTRKTKRETAAFAPFEKSADRQKPVS
                     VEPAFIARRVSKIYQMGDVQVHALNCVDLELYDGELVVLLGASGSGKSTLLNILGGLD
                     VPTSGEVFYRDHNLTIDDDAELTRYRREHVGFVFQFYNLIPSLTALENVELVTDIAEN
                     PMRPEEALQLVGLGERLHHFPSQLSGGEQQRVAIARAVAKRPEVLLCDEPTGALDYTT
                     GKLVLEAIERVNRELGTTTAIITHNAAIADMADRVLRMRSGQIALAQRNEKKAAPADL
                     VW"
     gene            6102..>6590
                     /locus_tag="VFV61_06615"
     CDS             6102..>6590
                     /locus_tag="VFV61_06615"
                     /inference="COORDINATES: protein motif:HMM:NF012893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HEX4900189.1"
                     /translation="MQNGTERRDWHAVEAPIVAADLGVKPASGLGLAEVAARRLRFGP
                     NSLQKIQPRPAWRLLIDQFKSIVIALLAVAAVVAWITGDILEAMAILVVLLINALVGF
                     LTEWQASRALDALRRQARTVTRVRRENFEATIDAAELVPGDIIILNAGDRVPADARLL
                     EAA"
BASE COUNT         1459 a         1939 c         1739 g         1453 t
ORIGIN      
        1 ccttagtttg ccagctatcc tgcagctggc gtagctgaat acaaagcctg tctcagtgcc
       61 acgtctcgcg ggtcgccatc aatcgctacg cccttgcggt tggggatata tccgtagccg
      121 atctgcgttt gcgggtcagc gaagccgagc gagccgccgg ctccgggatg accgaatgac
      181 cctggattgc cgatcggaaa tcccgaatca gttttcacaa agccgagcga aaactgcgcc
      241 tcgcctttta gacactcgtc gtagaaaccg tgcgtcgcgg gaattgccgg tgcgatcaat
      301 tcctgtaaag tctcctcgcg cagtcccagt tccttacctc cggtcgcaaa tacactgtag
      361 gcacgagcga tggctctcgc agtgccgacg cccccgcccg ccgggacttc caggttgcgg
      421 ctatagatcc gcttttcatc atgaggaaac tcggatccca tgagcgctct gcggatgttc
      481 gaccgatggt tccaaaccga cggcgtcagt cgaatgggaa acccgaacaa catctcgaag
      541 aagctgggat caacgagagt ggcgaacctc gaatcgggaa ttgattcggg caaacgaatg
      601 tagaaatcca accccagcgg tgaagctatt tcctcctgaa aaaattgtcc gaggctacgg
      661 tgttgcggat caatccggcg caacagttcg ctttcgtaat atccgagcgt aatagcgtga
      721 taagcctgtc gcgtgcctgg ctcccaggca ggtttctgtc gagccaatat ttcagccaga
      781 cggtcgagat cggcgactgt gcttctatca accggctcgt cgagggcgaa cagcccggcc
      841 tggtgggcca gtaactgccg cacggtaatt ctttcttttc cgttttgtgc gaactccggc
      901 cagtacttgc agacgagtgc atcgtaatcg agccagccgc gcgaatgggc gattgccaga
      961 gtcatcgctg ccaggccttt ggtcgccgaa tagaccaagg ccatggtgtt ttcttcccag
     1021 ggttcgccag tcgccttgtt acgcacaccg ccccagaggt caaccacttt ttctcctttg
     1081 tgataaatgc agcaggcggc accaatctcg tgtcgcttgg agaagttttc agcgaatgcc
     1141 tcgcgcaccg cttcatagcc gttgcttaca aagccgtgaa tgtcgacccg gctttgagcc
     1201 tcgtccgccg ccgcatgttt tctcttttca acccaaacat aagcggcgcc cgcagcgcct
     1261 gctgccgata agccaatgac tgccttcgtt aatgttttca tttcaactcc tctcttaaac
     1321 ttgcccatgg gcggtttgca gcaaggaaaa tggtgttttg aacgtgctca ttacttctct
     1381 tgcgcctcca cacggacgcc ctcactaatc tggttcgacg gatgcacgat aaccgtcgca
     1441 tcttccgcga gcccgctttt gatctctgct gcgaatggcg tgcgttggcc aacctcaact
     1501 tcgcgcaggt gtgcttcgcc attctcgacc acaaacagac tccaactttc tccacgacga
     1561 aatagcgcgc taacgggcac ttgcaggacg ttctctgcct cccacaccac gacacgaccc
     1621 tctacgcggt agccatcgcc gagctcggtt gaagctcttg ttaggtcagc aactacattg
     1681 actcgttgct cttcaattcc cagagcggag actttcgtaa acgccgaggg ttccatcaat
     1741 ctcactcgtg cttccaatga ttccgtccca ccccagtctt cgattaagac gagtgccccg
     1801 ggcctgactt tgaccgcatc cgtcgaaagc aattcgatta cgacctcgag catggaagga
     1861 ttgctcagtt cgactaatgg agcgccagcg gcaattactc tttcgctctc ctctacaagc
     1921 cttaacaccc ttccgcgcac tggcgcctga actttcacta ttgccgtggc cgctcttggg
     1981 ctttgatcgg ctgccagcaa tgccgcgctc gcgacttcga cttcgtgcgc tgcactttcg
     2041 gctctggacc gcgcggcttc atattcgcga aagctagttg ccacttctgt ttgtgctttt
     2101 tccaactcct gtcgggagat gtctccactg cgggcaagga tctcgcagcg gtcacaatct
     2161 cggcgcgcct gatcgtaaat ggctctgctg cgctcgatca ttctatcgac ttcgcgcttg
     2221 gtgtcctggg cagcgttgac gcgggcaatt gcctcagcac gttgccgcgg atccaaaggc
     2281 gtcaacggca gcgtttcaat cagcgccacc acctgccctt tctcaaccgc atcaccacgg
     2341 cgaagagtga tgcgttgaat ccttccggcc accggcgcag cgaccacata acgatcacgc
     2401 acacgagtct tgccttctcc atccacggtg acacgcagcg aaccgcgtgt ggttttcgcg
     2461 gtgtctactt tcgtgggctc cggacgcaac cacatcgtga cgagtgcgac gaccgccacg
     2521 gcaacaatga ttaagacgat ggtatttaag attcgtttca tggccattta ccccacagtt
     2581 attcccgcgt tttcaaaacc gctaccaaat ccaaatgtcg taaacgccag cgcaccagat
     2641 acccggaaag aaaggccgat cccgctacga cgagaaaagc gagcgtgtaa gtcttagcac
     2701 tgataaaaag tggaaaacgg atcaactcgg tatcgatcac aaccgtaatc agccaggcga
     2761 tcccatagcc caatgcgaac ccgagcggaa tcgccagcgc cgtcaggatt gcctgttcgc
     2821 ccagcaacat gaagccaatc tcgccgcggc tgaaacccag gacgcgcagc gaagcgagtt
     2881 cgtgcccacg ctctgacagc gctatgcggg cgccgttgta gaccatcccg aaagcgatga
     2941 ttacggcaaa cccaatcaga attacggtcg acacgttcat agtcctggcg atagtttcat
     3001 tgaagctagc caaggcaact tcaggaattg aaactgcact gactgcgggt gtctgcttta
     3061 gctcggaata cagttctggt attcgggctg attccaccat caggaacgcg ccgctgcttg
     3121 actgaccttc acccagcagc ttgttcaagg cccgaaggtt catgtaggcg gagagtccca
     3181 ccaattcatc gacagtcccc gccacggcga cgtcgcgcac gggcctggcg ccctcgagaa
     3241 cctcaacggt gacactttcg cccggcttta ctccaagaat ctcggcaagt tgcgttgtaa
     3301 gcagcaaacc gtccgccgga agtgtgacgt tttgcgaatc tttcgtaact atgcgccgca
     3361 agtcggatgc gctttccaat cccagcactg cggtgcggcg cgtccgatgt ccgacacgca
     3421 atcgtgccgg cacagtgcga taaggttcga cgcggatgac tcctggcagg tgagccagat
     3481 cgtatttcac actcgccggg cgtggctcgt ggaaagcaac tgaagcatct tcacgctgga
     3541 cctggtggaa ctggacgtcg atgatccgtc cgatagtatc gagatagaga aagaacccta
     3601 cgaccagcag acctaccgcg agagcgatac cgaaaatcga aaccattgcc tttccggggc
     3661 ggcgctccag attgcgaaga atgatccgca ccgaagtcgg tacgagttga tgaaaaccca
     3721 ggcgctcaat gaagccggag cgaaagcgcg ccgggggctc aggacgcatc gcttctgcag
     3781 gcggcaagct aacggctcgt ctgacagctg caagcgcacc gatcgccgct gagcccagac
     3841 tgattactac cgccccgaag attactgatg caccgggttg atagcgcaac acgggaaatc
     3901 tgaagaactc cgtgtagagc tcggtgatct tgaaaccaaa ataccagccg acagcgatcc
     3961 ccaaggcaac tccgctcagt acaactacaa atgcaagttt cagataatga aagccgatgg
     4021 aggagttccc atacccgaag gccttgagta ccgcgatctc ctgtctctgc gttgccacca
     4081 tgcgcgaaag caccaggtga atcaggaaag cagtgacggc aagaaaaatg gcgggaatga
     4141 aagtgccggt tacctgcaac tcggcgatct cattagaaag aaagcgatgg gaaatctgat
     4201 cgttgcgatt gtaggcgccg aagccgccgt aacttgccag tagttcgtcg acccgttcaa
     4261 gcacgtcggc ttcgaccgcg ccgggagcca gtgttagggc aacatcattg aacgcacctt
     4321 tcatgttgaa ggctggacca agcgcgtcac ggctcatcca gagcacggct gaacggcggt
     4381 ggtctggaaa catttcgccg ctcttgatct cataaacgaa ctctggagaa agtgctattc
     4441 ccacaatttg cagtttctgc catttgctgt ttatgactgc cgacaaggta cttcccggct
     4501 ttaggttgtt ggcggtagcg aaagcgccgc tcacaagaac ttcatctctc cgccctgatt
     4561 cgaggtagcg tccttcttga agatggagct cattcaacat cgggcgtcgt tcttctggaa
     4621 tggaaatgat tcttcctttt gcaggttcgt ccaggcctgg cacgtcaaga gttacatcag
     4681 ccacgacgcg aaactccgcc gcccccacgc cgggaatttc actaatgcgc atggcgattg
     4741 aatccggggc gcgcttgagt tgcgcaaaca cttctgcaaa gcgatacctt tcgtaataag
     4801 actgttgcga agccaggagc gcgttatagg tgctgcgcat tgccacaaag gaagcaacgc
     4861 cgcaggcaac taccagagtg atcgctgcca cttgtccgcg caggtgaatc acgtcgcgga
     4921 tgagttttcg attaagtgtg gccatgctca attagtgtcg cttaatcacc agaccaaatc
     4981 cgccggggcc gctttctttt cgttgcgctg cgccagtgcg atctggccgc tacgcatacg
     5041 caatacacga tcggccatat cggcaatcgc agcgttgtgc gtaataattg ctgtggtagt
     5101 tcccagctcg cgattaaccc gctcaatcgc ctccagcacc agcttgccgg ttgtgtaatc
     5161 gagggcgccg gtgggctcat cgcaaagcaa cacttccggg cgctttgcta ctgcgcgcgc
     5221 aatcgccact cgttgctgct caccgcctga gagctgcgaa gggaagtggt gcaatcgctc
     5281 tcccaacccg acaagttgca gggcttcctc cggtcgcatc gggttctccg caatgtccgt
     5341 caccagctca acgttttcga gggccgtaag actcgggatc aggttgtaaa actgaaacac
     5401 aaatccaacg tgctcgcgcc gataacgcgt taactccgca tcgtcgtcga tagtcaggtt
     5461 gtgatcgcgg taaaacactt cgccactggt cggaacatcc agaccgccga ggatatttag
     5521 cagcgtcgac ttgccactac ctgatgcgcc cagcaataca accagctcac cgtcgtagag
     5581 ttcgagatca acgcaattca aggcatgcac ctggacgtca cccatctggt aaatctttga
     5641 gactctacgt gcaatgaacg ctggctcaac actcaccggc ttctgtcgat ccgcgctctt
     5701 ttcaaacggg gcgaaagccg cggtttcacg tttcgtcttt cgggttagac atgcccgcgc
     5761 atcttctggt gtcgtcaccg ctgattggct caggtaagtc acacaaatcc tctcctctag
     5821 ctgttattcg aggtgcaaga gttgtacccg ctagggtggg aatcaaaacc gagagtttaa
     5881 gaaggattgg atctggaaag actttgctgg ttgcgagctt tcccgcagca gaagcggcac
     5941 ttttacccag tattctcttt acttccttag gaggtggccg cggataatta ggcaagcgcg
     6001 ggaaccggag cgaggcacat acattgcccc taatcaggct gatgtgtgtc gcccgaactt
     6061 ttgccgcttt gccacctgag cccgaagctg cgcccccctt gatgcagaac ggaactgagc
     6121 gacgagattg gcacgccgtg gaagcgccga tagtagctgc cgatctcggt gtgaagcccg
     6181 cgagtggtct cggattagcg gaagtagcag cacgacgtct tcggttcggt ccaaactccc
     6241 tacagaagat tcaacctcgc cccgcctggc ggctcttaat tgatcaattc aagagcattg
     6301 tgattgcgct tttggctgtg gctgctgtag tcgcatggat tactggagac attctcgaag
     6361 cgatggcaat tttggtggtg ctgctcatca acgccttagt gggtttcctg accgagtggc
     6421 aagcaagccg ggcgcttgat gcgctacggc gccaagctcg tacggtcact cgagtaaggc
     6481 gagaaaattt tgaagcgaca attgacgccg cggagctagt gcctggcgac atcatcatcc
     6541 tcaacgcggg cgatcgcgta cccgccgacg cgcgactgct ggaagcagcc
//
LOCUS       DASUEX010000393         2624 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1110204, whole genome shotgun sequence.
ACCESSION   DASUEX010000393 DASUEX010000000
VERSION     DASUEX010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1110204"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="VFV61_06620"
     CDS             <1..576
                     /locus_tag="VFV61_06620"
                     /inference="COORDINATES: protein motif:HMM:NF025875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HEX4900190.1"
                     /translation="AEINRSSWNTGQWVERQQPIQFSHKHHVGDDGIDCRYCHTSVEQ
                     SAPAGMPSSETCMNCHKQIWADSPYLEPVRASLRTGKPIEWIRVHDLPDYSYFNHSIH
                     VNKGVGCSTCHGRIDQMPIAYQASTLQMEWCLGCHRNPERFVRPKEKIFDMAWRPENR
                     SADEIASGRDLVAKYRIQKPDVLTSCSTCHR"
     gene            600..>2624
                     /locus_tag="VFV61_06625"
     CDS             600..>2624
                     /locus_tag="VFV61_06625"
                     /inference="COORDINATES: protein motif:HMM:TIGR04519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-variant-translocated molybdopterin
                     oxidoreductase"
                     /protein_id="HEX4900191.1"
                     /translation="MSVDDRHINFKLMREWILAHQDKEQQQQADEPLTTPVAEVSPNG
                     KKYWRSLEELADTPEFHEFVQREYPQHAEEWDDPIERRTFMKLMGASLALAGLSGCVY
                     QPPEKIVPFVRQPEEAVPGKALFFATAATVAGIATPLLIRSNEGRPTKAEGNPDHPYN
                     RGADVNDRGSSATDVFAQASILGLYDPDRSPTVTYREEIRTWTTFLGEIRTALDEQRP
                     KQGAGLRFLTETVTSPTIAAQLKGILTEFPQAKWHQYEPANRDNVRAGAIMAFGQPLN
                     TIYNFDQAKRILSLDADFLSAAPGTLRYARQFATGRRLVEGKQEMSRLYVVETTPTTP
                     GASADHRWAVKPSALEGIARRLAAKCGVSLPAEANATSNSDAAWVDAVARDLQQQKGA
                     SIVIAGNEAPPAVHALAHAMNAALGNVGNTVTYGEPLEANPVDQRESLRELVGDLDAG
                     RVELLVMLGGNPVYNTPADLKLDFNRLSKAKLRVHLSSHKDETSEVCHWHVPESHYLE
                     SWGDTRASDGTVCIVQPLIEPLYGSKTAHEVLAVFSDKYDRKAYEIVREYWQSQGAGF
                     GSRASGGGEAQQQEQTAATGQSSSAQPTASPVAADFEARWRKALHDGFIANSAPPVKT
                     VALNTAGLSGNAAVTSSSSVPNESRPSTSGTFEIVFRTDPNIFDGRFANNG"
BASE COUNT          606 a          749 c          737 g          532 t
ORIGIN      
        1 gctgaaatca atcgttcatc ctggaatacg ggccagtggg tcgaacgaca acaaccaatc
       61 cagttcagcc ataagcacca cgttggcgac gacggcatcg attgccggta ttgccacacc
      121 tcggtggagc agtctgctcc ggcaggcatg ccttcttcgg aaacgtgcat gaactgtcac
      181 aaacaaatct gggctgacag tccctatctg gaaccagtgc gcgccagctt gcgaacgggc
      241 aaaccgatcg agtggatcag agtgcacgac ctgcctgact attcttattt caatcacagc
      301 atccacgtga ataaaggtgt tggttgttcg acctgccatg gtcgcatcga ccaaatgccg
      361 attgcgtatc aggcttcaac gctgcaaatg gaatggtgtc ttggctgtca tcgcaatccc
      421 gagcgcttcg tgcggccaaa ggaaaagatt tttgatatgg cgtggcggcc agagaatcgc
      481 agcgcggatg aaattgcgag cggcagagat ttggtggcga agtatcgcat tcagaagccg
      541 gacgttttga ctagttgttc gacttgccat cgctagttgg cgctgtgggt ttaataaata
      601 tgtccgttga cgatcgacac ataaacttca agctgatgcg cgagtggatt ctggcgcacc
      661 aggataaaga acagcagcag caggccgacg agccgttaac aacgccggtc gctgaggtgt
      721 cgcccaacgg caagaagtat tggcgtagtc tcgaagagtt ggctgacacg cctgaatttc
      781 atgagtttgt gcagcgcgaa tatccgcaac atgcggaaga gtgggacgac ccgatcgagc
      841 gccgcacgtt tatgaagttg atgggcgcat cgctcgcact cgcgggcttg agtggctgtg
      901 tctatcaacc gccggagaag atcgttccgt ttgtccggca accagaggaa gcggttcccg
      961 gtaaggcgct gttcttcgcg actgcggcca cagttgccgg aatcgctacg cccttgctga
     1021 tcaggagcaa tgaaggccga cccaccaaag ccgaaggtaa cccggaccat ccttataatc
     1081 gcggcgccga cgtcaacgat cgcggttcca gcgccaccga tgtgttcgct caggcttcaa
     1141 ttcttggcct ctacgatcct gatcgctctc cgacagtgac ataccgggaa gaaattcgaa
     1201 cctggaccac ttttctcggc gagatacgca cggccctgga tgaacaacga ccgaagcagg
     1261 gcgccggtct tcgtttcctc accgaaacgg taacgtctcc aacaatcgcg gcccagttga
     1321 aaggaatcct gacagagttc ccccaggcaa agtggcatca gtacgaacct gcaaatcgcg
     1381 acaacgtgcg ggcaggcgca atcatggctt tcggccaacc gctcaacacg atctacaact
     1441 tcgatcaggc aaaacgaatt ctttctctcg atgccgactt cctctcagca gctcccggaa
     1501 cacttcgtta tgcgcggcag tttgccaccg gtcgccgtct cgtcgaaggc aaacaagaga
     1561 tgagccggct ctatgtcgtg gaaacgacgc cgacaactcc cggcgccagc gcggatcatc
     1621 gttgggcggt gaaacccagc gcgctggagg gcattgcgcg tcggctggcg gcaaaatgcg
     1681 gggtctcgct gcctgcggaa gcaaacgcca caagcaacag cgatgctgcc tgggttgatg
     1741 cagtcgctcg tgacctgcag caacaaaagg gcgccagcat cgtcattgcc gggaacgaag
     1801 ccccgccggc agtccacgcc ttagcgcacg caatgaatgc tgcgctcggg aatgtgggca
     1861 acaccgtaac ttacggcgag cccctcgaag ccaatcctgt cgatcagcga gagtctttac
     1921 gtgagttggt tggagatctc gatgccggcc gcgtggaact gctcgtcatg ctaggcggca
     1981 atccggttta caacacgccc gctgatctaa agctcgattt caaccgcctc tcaaaagcta
     2041 aacttcgggt tcatctcagc tcgcacaaag atgaaacgtc cgaggtttgt cattggcatg
     2101 tgcctgaatc tcattacctc gagtcgtggg gcgacacgcg cgccagcgac ggcacagttt
     2161 gcatcgtcca gccgttgatc gaaccactct atggttcgaa gacggcacac gaagtattgg
     2221 ctgtcttttc cgacaagtac gatcgcaagg cttatgagat cgtgcgcgag tactggcaaa
     2281 gccagggggc gggtttcggg tcacgcgcat cgggtggcgg tgaagcgcaa caacaggaac
     2341 agacggcagc taccggccaa tcgagttccg ctcaaccgac agcttcaccc gtggcggcag
     2401 atttcgaagc tcgctggcgt aaggcgctgc atgatggctt catcgccaac agtgcaccgc
     2461 cagttaagac agttgctttg aataccgccg gattatcggg caatgctgcg gtgacgtcct
     2521 cttcttcagt accaaacgag tcccggccct cgacctcggg caccttcgaa attgtattcc
     2581 gcacggaccc aaacatcttt gacggtcgct tcgcaaacaa cggc
//
LOCUS       DASUEX010000394         2757 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_639153, whole genome shotgun sequence.
ACCESSION   DASUEX010000394 DASUEX010000000
VERSION     DASUEX010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_639153"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1465)
                     /locus_tag="VFV61_06630"
     CDS             complement(<1..1465)
                     /locus_tag="VFV61_06630"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT and VWA domain-containing protein"
                     /protein_id="HEX4900192.1"
                     /translation="MRSKTLLFISLLFLVVSFAKSFPVSAQGVIVPGPCQRCPPPQRE
                     MPPITLPRTLPIKSIKIDTKIVSQVATTHLEQVFRNDTNATMEGTYLFPIPEAASIVE
                     FAIWDGDRRLVGEVRSREEARRIYDEIVRRQRDPGLLEYAGKDLFQASIFPIAPHSDK
                     KLELTYSQVLRAEAGTVAYRYPLGTGRQLTAIGSVSGRVVLESKDPVRNIYSPSHAIS
                     VTRSGERNSAISFESVTGQEPEDFRLFYTLSNLDFGVSLLTHREAGKDGYFLLMISPK
                     DDWREQEYAAKDIVFVLDTSGSMAETGKMEKARAALLYGIRILRPKDRFNVISFAGEE
                     RLFETGLIAADDEGRKRGEAFVQALRPVGGTNINEAVLAAMRQFRVSDRPRMIVFMTD
                     GLPTAGETNPTRILDNAIQSRVPGVRLFSFGVGYNVNTALLDKLAADNGGVADYIEPN
                     EDLEAKVSTFFARVNSPVLTDLRLDLAGVETDLVYPRA"
     gene            complement(1656..>2757)
                     /locus_tag="VFV61_06635"
     CDS             complement(1656..>2757)
                     /locus_tag="VFV61_06635"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4900193.1"
                     /translation="LTDSAFWLGFDGFMATVPGFFLTLAGGVFADLVDRRRLLRYTQI
                     VAGIAAFALAVLVSSHLVNLYMVLAFSFVTGCCMAIAGPSYQAMTYDLVGREDLANAV
                     ALNSSQFQLSRVVGPVAAALGFKFFGLAGCFYANGVSFIAVVIALSLVRFDERKTIAH
                     SVKDRRALWQDLVDGLRYVRKRPRVSSLLSISAATSLFGAPYLALIPIFARDIFHLGA
                     TGLAWMMGTAGAGAFLGALVLAYLGDFKRKGWSVLGGAFFFGICLIVFAQSTWLPLSL
                     IFLFGVGFAIVTSVAVTNTLLQKLVTDRMRGRVMSMFILSFVGAMPIGNLLAGVVSHR
                     FGAPAAITAGGLAIVIYISVMTLTNKRLRDLH"
BASE COUNT          658 a          774 c          780 g          545 t
ORIGIN      
        1 aagctcgcgg ataaaccagg tccgtttcca ctcccgccag atccaggcgc aagtcagtca
       61 acacggggga gttcactcgc gcaaagaaag tcgaaacttt cgcctcgaga tcctcattcg
      121 gttctatata gtcggccacg ccaccattat cggctgccag tttgtccaaa agggctgtgt
      181 tgacgttgta gcccacaccg aaactaaaga gtcgcacgcc gggaacacga gattggatgg
      241 cattgtcgag aatgcgcgtc ggatttgtct caccagccgt cggcagaccg tcggtcatga
      301 agacgatcat cctgggacga tcgcttaccc ggaattgacg catcgcggcg agcactgctt
      361 cgttgatgtt cgtcccacca acaggtcgca gtgcttgcac aaatgcctca ccgcgtttgc
      421 ggccctcatc gtccgctgcg atcagacccg tctcaaacaa acgttcctcg ccagcgaatg
      481 agatcacgtt aaagcgatct ttgggtcgca gaattctgat tccataaagc agcgctgcgc
      541 gtgccttttc catctttccc gtttcggcca tggagccgga ggtgtcgagc acgaagacta
      601 tgtccttcgc tgcgtactcc tgttcccgcc aatcgtcctt gggagaaatc atcagcagaa
      661 agtaaccgtc cttgcctgct tcgcgatggg tcagcagcga cacgccgaag tccagatttg
      721 agagcgtata gaagagtcga aagtcttctg gctcttgtcc ggtaacggat tcgaacgaaa
      781 ttgctgagtt gcgttcgcca ctgcgcgtaa ctgaaatcgc gtgcgatggt gaatagatat
      841 tacggaccgg atctttgctt tccagcacca cccggccgga aaccgagccg atagcggtca
      901 actgacgacc ggttccgagc ggataacgat acgcaaccgt tcccgcttca gcacggagta
      961 cttgtgagta ggtaagttcg agctttttgt cggagtgcgg cgcaatggga aagatactgg
     1021 cttgaaagag atccttgccg gcatattcaa gaagaccggg atcacgctgg cgccgaacaa
     1081 tctcgtcata gattcgtctc gcctcttcgc gcgagcgtac ttctcctacg aggcgacggt
     1141 cgccgtccca gatcgcgaac tcgacgatcg atgctgcctc tggaatcgga aacaggtaag
     1201 tgccttccat cgtcgcgtta gtatcattcc tgaacacctg ttccaagtga gttgttgcta
     1261 cctgggaaac gattttggtg tcgatcttga tggacttgat cggcagggtc cgaggcaagg
     1321 ttatcggggg catttcccgc tgcggtggcg ggcagcgctg gcaagggccc ggcacgatca
     1381 cgccctgggc gcttacgggg aagctctttg caaaagagac gacgaggaac aggaggctaa
     1441 tgaacaggag tgttttgcta cgcatactga cctcaagagt ggatttgagc gcgggcatct
     1501 cgagtttaga acggactaac ggcccggtct tgcgtgatgg ggagaagctt ttccggcgac
     1561 ttggctgttc tggcgtccag agcgcggact ggccgatcac ttaccgctgc tgtggccgcc
     1621 ggtcccggcc ctttgcatcc gctgcgcagg ttggctcagt gcaagtcgcg gagtctctta
     1681 tttgtaagag tcataaccga gatgtagatg acgatcgcga ggcccccggc agttatcgct
     1741 gctggggctc cgaaacggtg ggagacaacg cccgctagta gatttccaat cggcatcgca
     1801 ccaacgaatg aaagaatgaa catgctcatt acgcggccac gcatccggtc ggttaccagt
     1861 ttttgcagca acgtattggt cacggccacc gaagtgacaa ttgcgaagcc gacgccgaaa
     1921 agaaagatga gtgataaggg caaccaggtt gactgggcaa acacgattag acaaatgccg
     1981 aagaagaatg caccaccaag aacggaccag cccttcctct taaaatcgcc cagataggca
     2041 agcaccagtg cccccaggaa cgccccggcg cccgccgtcc ccatcatcca ggccaagccg
     2101 gtcgcgccca gatgaaaaat atcgcgagca aagatcggaa taagcgccag gtacggtgcg
     2161 ccgaaaagac tcgttgctgc cgagatcgaa agcaatgacg acacgcgggg acgttttcta
     2221 acgtaacgaa gtccatccac caggtcctgc cagagtgcgc gacggtcctt taccgaatgc
     2281 gcaatggtct ttcgttcgtc gaagcgaacg aggctgagcg cgatcaccac cgcgataaaa
     2341 gaaactccat tggcgtaaaa gcatcctgcg agtccaaaga atttgaatcc cagtgctgcg
     2401 gctacgggac cgactacgcg cgagagctga aactgtgatg agttgagcgc gacagcattg
     2461 gccaggtctt cgcggcccac cagatcgtaa gtcatggcct ggtaagacgg cccggcgatc
     2521 gccatgcaac agccagtcac gaacgagaag gccaacacca tgtacaggtt cacgaggtgc
     2581 gagcttacaa ggacggcaag agcgaaggcc gcgattcccg caacgatctg cgtgtagcgg
     2641 agcagccggc gcctatcaac cagatccgca aaaacacccc cggcgagcgt tagaaagaat
     2701 cccggcaccg tcgccatgaa accgtcaaag ccgagccaga aagcggagtc ggttagc
//
LOCUS       DASUEX010000395         3424 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_292856, whole genome shotgun sequence.
ACCESSION   DASUEX010000395 DASUEX010000000
VERSION     DASUEX010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3424
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292856"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(160..3285)
                     /locus_tag="VFV61_06640"
     CDS             complement(160..3285)
                     /locus_tag="VFV61_06640"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX4900194.1"
                     /translation="MNSDPTDESLEQELEKQFPSARSAAERQSVLELTRGLTRLPLNI
                     STAILETSAAIGGGSLRAALEFLRAAPAAAEVLEVEELRAWGEMGRRLAMGDVETSIS
                     FFIAGVEDLRDVPRSAHPLLFQACTRQMTLSAANATDTLRRARQLSASLDDPRVLNTV
                     LEIAAEIARRSAKHSSDFLAATPDVVAALRKFADPAVLEQAFQLASAFAFRAGGMAAD
                     AWSAIPTALGNLDDKNALRLIASTDDFLERGGGGALNLMLAGGEMLRLAPGLFSDWIE
                     LLRVVAQHGNASLVAFVRASPRFVRGFFSDQKSADSISLMKRVIALTREIAGVDGEAA
                     LACFRSSPGVLRTVAIEQFAEWAQNGLATTRNDARARRSYFALETRSSYAVLHSGSAG
                     LSLDAVKEVLRLYVEALTGQVIEIAPIAAVPMETRIGDGKTIHLPSLVAEFGDADLDF
                     RLYKVLAAHAAGQIEFGTYQRNTSGLSAAFAAISATYDPENAGEQDAFALNGYLSDLP
                     ATAGSGEAATAAPNDFDYSAVLRLFPDPPLAQRIFGTLENGRIDYQLRHKYRGLARDL
                     DLVREHLRPTRPKITDLPATLLPFEILFQITMLGGALADARQFYGQIVSELETIFAEY
                     LTSPAATVADTLMATRRAYQLFQSVVPTDDSVQQLEVEKEPAESDRESSMISESLRNR
                     RDDRQPQRRDARDLFNAWNDPNAEGDPDNLEGGDAWIEGESAELELQEGEVAYNYDEW
                     DRELTDHRLGWCRVIEKKVKHGDRSFVDDTRERHRGVISSIRYQFQLMKPEELTRVAN
                     EIDGEEYDLNAVVDYVLDRRAAKVGGGHQSERLYTKRLRRRRDVAVSFLLDQSSSTAR
                     TIGRHPLQPYTHPGRRIIEIEKEGLVLMSEALEAVGDVYAIHGFTSEGRRNVKFYVVK
                     DFSERYSDEVKQRIGGITYQNNTRLGAAIRHATAKLANQQARTRLLIVLSDGRPYDHD
                     YGDARYAREDTREALRQAKIQGITPFCITIDRESESELKDLYGEIGYTIIDDVLSLPE
                     RLPGIYRRLTT"
BASE COUNT          736 a          963 c          992 g          733 t
ORIGIN      
        1 agctgggttg gttctcctac tggaaaggaa gacgagagag ctaatttcgc tgaggtttag
       61 tggtctggaa gttgagcggg ggcgcgcttc gcttgccccc ttcctgacag gtaaatttct
      121 gtaggttgga tagttcaatg ctagatcact atttaaagct cacgtcgtca acctgcgata
      181 gattcccggc agtctttctg gcaacgaaag cacatcatca atgatcgtgt agccaatctc
      241 gccgtaaaga tcctttaact ccgactctga ttcgcgatcg atggtgatgc aaaatggagt
      301 aatgccctga atcttcgctt gtcgtaacgc ttcgcgcgtg tcttcacgtg cgtaacgcgc
      361 atcgccatag tcgtgatcgt aaggacgacc atccgaaagc acaatcaaca aacgagtgcg
      421 agcctgctgg tttgcgagct tcgccgtcgc atggcggatc gccgctccca accgcgtgtt
      481 gttctggtaa gtgatgccgc cgatccgttg cttcacttca tcggagtagc gctcggaaaa
      541 atctttgacg acgtaaaact ttacgttgcg ccgtccctcg gaagtgaagc catgaatcgc
      601 gtagacatcg ccgaccgctt cgagcgcttc actcatcaac accaaccctt ctttttcgat
      661 ctcgatgatg cggcggccgg gatgcgtgta aggctgaagc ggatgacgtc caatcgtgcg
      721 cgccgttgaa gaagattgat cgagcaggaa agaaacggcg acgtcgcgcc ggcgtcgcag
      781 gcgcttggta tacaggcgtt cagattgatg gccgccgcca actttggctg cgcggcgatc
      841 gagtacgtaa tcaacgacgg cgtttagatc atattcctca ccatcgattt cattcgcaac
      901 gcgcgtcagc tcttcaggct tcattagctg gaactggtag cgaattgatg aaatcacgcc
      961 gcggtgccgt tcgcgagtgt catcgacgaa actgcggtcg ccgtgcttta cttttttctc
     1021 gatgacgcgg caccacccca gccggtgatc cgttaactcg cgatcccatt cgtcgtagtt
     1081 gtaggcaact tcgccttctt ggagctccag ctccgcgctt tcaccttcga tccaagcgtc
     1141 gccgccttcg agattgtcgg gatcgccctc agcattagga tcgttccagg cgttaaaaag
     1201 atcgcgcgcg tcgcgccgtt gcggctggcg atcgtcgcgg cggttgcgca gcgactcgct
     1261 tatcattgag ctctcgcggt cgctctccgc tggttccttt tccacttcaa gttgttgtac
     1321 tgaatcatct gtaggaacta cagactgaaa gagctgatac gcgcgtctcg tggccatcag
     1381 cgtgtcggcc accgtcgcgg ccggactggt caggtattcc gcaaaaatgg tttccagctc
     1441 agaaacgatt tgaccgtaaa actgtcgcgc atcagcaagc gcgccaccca gcatcgtgat
     1501 ctgaaagaga atctcgaaag ggaggagcgt tgccgggaga tcggtgatct tagggcgagt
     1561 cgggcgtaga tgttcgcgaa cgagatcaag gtcgcgcgcg aggccgcggt acttgtgccg
     1621 taactggtaa tcgatgcgac cattctccag agttccaaag atacgttgag ccaaaggcgg
     1681 gtcaggaaag agacgcagca cggcagaata atcgaagtca ttgggagcag cggtggccgc
     1741 ttcacccgag cctgcggtcg ccggcagatc ggagaggtac ccattaagcg caaaagcatc
     1801 ctgctcgcca gcgttttcgg gatcataagt ggctgagatc gctgcaaacg ctgcgctgag
     1861 accggaggtg ttgcgctggt aagtcccgaa ctcaatttgt cccgcggcgt gagcggccag
     1921 gaccttgtac aagcggaaat ccaaatccgc atcgccaaac tccgccacga gcgatggcaa
     1981 gtgaattgtt tttccgtcgc caattctggt ttccattggt accgcggcaa tcggggcaat
     2041 ctcgattacc tgaccagtca acgcttccac gtaaaggcgc agcacttctt tgactgcgtc
     2101 cagcgacaga ccagcactgc cgctgtgtaa caccgcgtag ctactgcgcg tttccaaagc
     2161 gaaataactg cgacgtgcgc gcgcgtcatt cctggttgtt gccagaccgt tttgcgccca
     2221 ctcggcaaac tgctcaatgg ccaccgtgcg cagtacacca ggactggaac gaaaacaagc
     2281 aagggcggct tcgccatcca cgccggcgat ttccctggtc agtgcgatca cgcgcttcat
     2341 gagcgagatt gaatccgcag acttttgatc actaaaaaag ccccggacaa aacgcggact
     2401 ggcccgtaca aaagcaacga ggctggcgtt gccgtgttgg gccacaactc tcaaaagctc
     2461 gatccaatcg ctgaacagcc cgggcgccag gcgcagcatt tcgccgcccg ccaacatgag
     2521 gttcaaagcg ccgccaccgc ctctttcgag aaaatcgtcg gtgctggcaa tcagtctgag
     2581 cgcgtttttg tcgtcgagat ttcccagcgc tgtgggaatg gctgaccagg catcagcggc
     2641 catgccgcca gcgcgaaagg cgaacgctga cgcgagctga aacgcttgtt ccagtacggc
     2701 cgggtcagca aatttcctta aggcggcgac aacatccggc gtcgcggcga gaaaatctga
     2761 gctgtgtttg gcagagcgcc gggcgatctc agcggcaatc tcgagcactg tgtttaggac
     2821 gcgaggatca tcgagggaag ctgacagttg tcgagcccgg cggagtgtgt ctgtcgcgtt
     2881 cgcagccgaa agagtcattt gtcgggtgca ggcttgaaac agaagcggat gggcgctgcg
     2941 cgggacgtcc ctgagatctt cgacgccggc aatgaagaaa gagattgagg tttccacgtc
     3001 gcccatggcc aaacgacggc ccatctctcc ccacgcgcgg agctcttcga cttcaagcac
     3061 ctcggctgct gcgggtgcag cgcggagaaa ttccagagcc gcgcgtaagg aacccccgcc
     3121 gattgccgcg gaggtttcca gaattgcagt ggagatgttg agtggcaggc gggtgaggcc
     3181 gcgcgttagc tccaagaccg attgtcgttc cgcagcgctg cgagcagaag ggaattgctt
     3241 ctccagttcc tgctcaaggg actcgtccgt tggatcggaa ttcatttatt ggagagtcag
     3301 aaggttcgcg tgcgaagcac gcgaaggcgt aaagcctggg gcgtaagccc caggaacgca
     3361 gtttgtttca accccctagc ccgcacagcg ggcgacggat tgtttgccca tactgtcgcc
     3421 cgct
//
LOCUS       DASUEX010000396         4207 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_639299, whole genome shotgun sequence.
ACCESSION   DASUEX010000396 DASUEX010000000
VERSION     DASUEX010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4207
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_639299"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..1347
                     /locus_tag="VFV61_06645"
     CDS             118..1347
                     /locus_tag="VFV61_06645"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX4900195.1"
                     /translation="MTQPLTVVILSTGLENFKELRNALSGDSRIQLLAGGDDADQLHD
                     EIMRLKPMAAIISLGAEAEQGIKFIKRLNSEFPKIAIVSAAHNASRDLLLQSLRAGAR
                     EFLPLPVVAPELGTVIDRIAEFCHSHVEAPKKKGRMIAVFSSKGGCGTSFLATNIAAC
                     SGAPTILVDLNLQAGDLPLFLGVNPKYSFADMAENRNRLDDELIKSFATPYSSNLSLL
                     AAPREADSADEIEPEHVFDVLQRLRESYEYVVLDPQHTFDSITLAALDQSDEILLVLS
                     LDIPAIRSTQRALEIFDRLGYPRKKIRIVVNRWSKQIDLDLQQVEKFLGEPVIGFVPS
                     DYQTAVSSINLGQPLVHSDGNSRIALEIKRVARIVSGGAVPVTDVTNERRTFWNSLFR
                     REPVQSQFELRTSLEKV"
     gene            1377..2711
                     /locus_tag="VFV61_06650"
     CDS             1377..2711
                     /locus_tag="VFV61_06650"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HEX4900196.1"
                     /translation="MALRERLIRETAGLPGVPGNGENAQYSFQEMKAKLHRSLINRMD
                     LTKLGALSTEQAHAEVSRLAESVLAQEELPLSAADRERLVNDVRHELFGLGPLEPLLA
                     DPTISDILVNAHGTIYIERRGKLEVTSVAFKDDEHLMRVIERIVTSVGRRIDESSPMV
                     DARLRDGSRVNAIIPPLSIDGPVLSIRRFGAEPLRMHMLIEHKALTKDIADMLQMCVT
                     ARLNVLISGGTGAGKTTLLNALSAYIPEDERIVTIEDSAELQLQQPHVVRLETRPPNI
                     EGRGEVTQRDLVRNSLRMRPDRIVIGEVRGGEAIDMLQAMNTGHDGSLTTIHANTPRD
                     ALARLETMIQMTGMRLSDRAMRQQVASAIDLVVQVARLSDGTRRLTSISEITGMEGET
                     ITMQEIFLFERTGVDSSGQVIGRFKPTGIRPRFAERLKACGLQLPRVFFEEM"
     gene            2717..3682
                     /locus_tag="VFV61_06655"
     CDS             2717..3682
                     /locus_tag="VFV61_06655"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX4900197.1"
                     /translation="MLISFLVFLFGLFLVLGAYLIATRGSDSKRRRLQKRLADALLHS
                     AHTEDVEVILARNELMSEIPFVNRFLVQLQAALHLKRMLDQADIQITPSRMVMFSVMA
                     GMLGLLAASALTISVLIIAASGLVAAMIPFVHVWWKRRQRFNAFLEHLPDALDLMSRG
                     LSAGHAFSESLHMVASEMPEPIATEFRKTYEEQNLGLSLKLALENMIDRVPLLDLKMV
                     VTAVLIQRETGGNLAEILEKVAYTIRERFRIMGDLKTLTTSSRMSAWLLCGLPIFVTF
                     VITAMNPEYMSVLWKDQRGHYLIVTALFLQVTGMLIVRKILNIKI"
     gene            3840..>4207
                     /locus_tag="VFV61_06660"
     CDS             3840..>4207
                     /locus_tag="VFV61_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900198.1"
                     /translation="MLLIITISTFICISLIMMGAYWLLYRPQSAATERLKRLGDRVTG
                     PVTGTVSIEERQRSSEIAERLASPLNKLLPPSAAEVKKLQKQLMQAGFRSPGAPASFR
                     AIQFVTMAGFPAIVALICAVT"
BASE COUNT          969 a         1168 c         1130 g          940 t
ORIGIN      
        1 gacgatcaac atgcctgatt acgaatcaac actcaccggc gaaagcgccg gcaccgtccc
       61 ctaaaccagg tgctccagcc ccactaactg gtttacttca acctcaagag caacgaaatg
      121 acacaaccat taacagtcgt aattctcagt acaggcctgg agaacttcaa agagcttcgc
      181 aacgcgctct caggggatag tcgtattcaa cttcttgccg gcggcgacga tgccgatcag
      241 ttgcacgacg agatcatgcg cctcaagcca atggccgcca tcatttccct tggcgccgag
      301 gccgaacaag gtatcaagtt catcaagcgc ctcaattcag agtttccgaa gatagcgatc
      361 gtttcagcgg cgcataacgc gtcccgcgat ttgctgctgc aaagcttgag agcgggcgcg
      421 cgtgaattct tgccattgcc ggttgttgcc ccggaactgg gaacggtcat cgatcgaatt
      481 gctgagtttt gtcattccca tgtcgaggcg ccaaaaaaga aaggtcggat gatcgccgtc
      541 ttttcgagca aaggtggatg cggtacgtca ttcctagcta ccaacatcgc ggcctgctcg
      601 ggcgcaccga cgattttggt tgacctcaat ttgcaagcgg gtgatctgcc actctttctg
      661 ggcgtcaatc cgaaatattc attcgccgac atggcggaga atcgcaaccg gctcgacgat
      721 gagctgatca agagtttcgc cacaccctac tcttcaaatc tttctttgct ggccgcaccg
      781 cgggaagctg actcggcaga tgaaatcgag ccagaacatg tctttgatgt gctgcaaaga
      841 ttgcgtgagt cttatgagta cgtggtcctc gatccgcagc acacgtttga ctcaatcacc
      901 ctcgctgcgc tcgaccagtc ggacgagatt ctgctggtct tatcgctcga cattccagcg
      961 attcgcagca cccaacgtgc gttggaaatc tttgaccggc ttggttaccc gcggaagaag
     1021 attcggattg ttgttaaccg ctggagcaag caaatcgatc ttgacttgca gcaagtcgag
     1081 aagtttttag gcgagccagt tataggtttt gttcccagcg attaccagac cgcagttagc
     1141 tcaattaacc tcggccaacc tctcgtccac tcggacggca actcgcgcat tgctctggag
     1201 atcaagcgag tagcgagaat cgtgtccggt ggtgcagttc cagttacaga cgtcacaaac
     1261 gaacgccgca cgttctggaa ttcactgttc aggcgtgaac cggtgcaaag ccaattcgaa
     1321 ctgcgcactt ccttggaaaa ggtctaaatc aaacaaactt taggaaaagg taaaccatgg
     1381 cactcaggga aagactaatt cgcgagacag ccggacttcc gggagttccc ggaaacggtg
     1441 aaaacgccca gtactccttc caggagatga aggcgaagtt gcaccggtcg ctgatcaatc
     1501 ggatggacct gaccaaactt ggcgcgctct ccaccgaaca ggcccacgct gaagtctccc
     1561 gattggccga gagcgtcctg gcgcaggaag agctgccgct ttcggcagca gatcgtgagc
     1621 gcctggtaaa cgatgttcgc cacgagttgt ttggactggg accgctggag ccgctgcttg
     1681 ccgatcccac catctccgac attttggtca atgcgcacgg cacgatctac atcgagcgcc
     1741 gcggcaagtt agaagtcacc agtgttgctt ttaaggatga cgagcacttg atgcgcgtta
     1801 tcgaacgcat cgtcacttcc gttggccggc gcatcgatga gtcgtcaccg atggtggatg
     1861 cccgtctccg ggatggctcg cgcgttaacg cgattatccc tccactttct atcgacggcc
     1921 cggtgctgtc gattcgccgc tttggcgccg aaccgctgcg gatgcacatg ctcatcgagc
     1981 acaaggcgtt gacgaaagat attgccgaca tgctgcagat gtgtgtcacg gcgcgcctga
     2041 acgttttgat ttctggtggt accggcgctg gtaagactac tctgctaaac gccctctccg
     2101 cttacattcc cgaagacgaa cgtatcgtca cgattgagga ctccgcagaa ctccagctac
     2161 agcagccgca cgtggtgcgc ctggaaacga gaccgccaaa tatcgaagga cgtggtgaag
     2221 tgacgcagcg cgatctggtg cgcaactcac tgcgtatgcg acctgaccgt attgtcattg
     2281 gtgaggtacg tggcggcgaa gccatcgaca tgctccaagc catgaacact ggtcacgacg
     2341 gaagtttgac tacgatccac gcgaatactc cccgtgacgc gctggcccgc ctcgagacga
     2401 tgattcagat gaccggcatg cgactctcag atcgcgccat gcgtcagcaa gttgcctcgg
     2461 ctatcgactt ggtagtgcag gtagcgcgcc tgtcggatgg cacccgaagg cttacttcaa
     2521 tttctgaaat cacaggcatg gaaggcgaga ccattaccat gcaggagata ttcctctttg
     2581 agcgcacggg cgtcgattca tccggccagg tgatcggtcg cttcaaaccg accggcattc
     2641 gcccgcgctt cgccgagcgc ctcaaagcct gtggactcca actgccccga gtcttctttg
     2701 aggagatgta aaccaaatgc tgatctcgtt tttagtattc ctctttggtc tcttcctggt
     2761 gttgggggct tacctaatcg ctacccgcgg gagcgattcc aaacgaaggc gcctgcaaaa
     2821 acggctcgct gacgcgctgc ttcactcggc gcataccgag gatgtcgaag tgatcctcgc
     2881 ccgcaacgag ttgatgagcg agattccgtt cgtgaatcga tttctggtgc agctccaggc
     2941 agcgttgcac ctgaaacgaa tgctggacca ggccgatatt cagatcacgc cttcgcggat
     3001 ggtgatgttc tcggtgatgg ccggcatgct gggtttactg gcggcgtcgg cgctcacaat
     3061 atcagtctta atcattgccg cctccgggct tgtcgccgca atgattccgt ttgtccacgt
     3121 ttggtggaaa cggcggcagc gcttcaatgc ctttctcgag cacctgccgg acgctctcga
     3181 cctgatgagt cgcgggctta gcgccggcca tgctttttcg gaatcactgc acatggttgc
     3241 gagcgaaatg ccggaaccga tcgccacgga gtttcgcaag acctacgagg aacaaaacct
     3301 cggtctttca ctcaagttgg cgctggaaaa catgatagat cgcgtgccgc tgttggatct
     3361 gaagatggtt gtaaccgcag tgcttattca gcgagagacg ggcggaaacc tcgccgagat
     3421 cctcgagaag gttgcctaca caattcggga acgcttccgc atcatgggtg atctgaaaac
     3481 gttaacaaca agttcgcgca tgtcggcctg gttgttatgc ggattaccaa tcttcgtgac
     3541 gttcgtaatc acggcgatga atcccgaata catgagtgtg ctgtggaagg atcagcgagg
     3601 tcactactta atcgtaacag cgctgtttct gcaagtcacc ggaatgctga ttgtgagaaa
     3661 gatactcaat ataaagatat gaacttggag ctttgtgctt tgaactttgt tgcatctggc
     3721 gcaagttagg cagctccgaa aagacaagca ccgaagtaca aagaacgaac gcaaagaacc
     3781 aacgaagaac aaagtacaaa gttcaaagaa caaatcagcc caagcttccg agaccagcca
     3841 tgttactcat aatcacaatt tccactttca tctgcatctc cctgatcatg atgggagcgt
     3901 actggttgct ctaccggccg cagagtgctg ccactgagcg cctgaaacgg ctgggcgaca
     3961 gagtgacggg tccggttact ggcacagtct cgattgaaga acgtcagcgt tcatctgaga
     4021 tcgcggaacg actcgcatct cctctgaaca agctgctgcc gccttccgcg gctgaagtga
     4081 aaaaacttca gaagcagcta atgcaggcgg gctttcgctc acccggcgcc ccggcaagtt
     4141 ttcgcgcaat ccaatttgtg actatggctg gcttcccggc gatcgtggcg ctcatctgcg
     4201 cagtgac
//
LOCUS       DASUEX010000397         4744 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1202662, whole genome shotgun sequence.
ACCESSION   DASUEX010000397 DASUEX010000000
VERSION     DASUEX010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4744
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1202662"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2510)
                     /locus_tag="VFV61_06665"
     CDS             complement(<1..2510)
                     /locus_tag="VFV61_06665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX4900199.1"
                     /translation="MLQGESVRSFIKTYALGIVALGVAVLLRWLLDPLMGDALPLVTL
                     FGAIAAAVWLGGYRPAIVVTILGYLACHYLFIEPRGKLDLNHLADVVGLVAYLFTCAL
                     IIAFGESARFAQQRATERREILSVTLKSIGDAVITTDNAGHITYLNRVAESVTGWALA
                     DALGQPLDKVFHIVNEATRLPVENPATKALHQGIVVGLANHTLLIKKDGSECPIDDSA
                     APIRDEQGHVSGCVLIFRDVSAQRLVEREKANQLHTARLLASIVESSNDAIIGKSLAG
                     VIQSWNAAAERLFGYSAAEAVGQHISLVIPAERISEEDEILARLKAGQRIEHFETERV
                     RSDGQRILVSLTISPIKDEAGNVVGASKIARDITDRKRVEADREKFATLVESSTDFIG
                     MCDLDGVPFFVNRAGLEMVGLDSIEEARNTPVADFFFPEDQDRIMNQFFPAVMEQGHG
                     EVEIRFRHFKTGTARWMAYKVLKLSDANGEPIGFATVSQDVTERRRLADDLKRLAADL
                     SLADQRKNEFLATLAHELRNPLAPLVNMLEVLKRADGDGETLGRARDTIERQLGQMVR
                     LVDDLLDLNRITHDRLELRQSEVELASVIHQAVEVARPLLDSANHDLRLALPAEPIYL
                     NADPARLAQVFGNLLSNSCRYTKPGGTIWVGAKREDSEVVVTVKDTGAGIPQDKLASI
                     FDMFTQVDRSPERSQGGLGIGLTLVKRLVEMHGGSVEAKSAGEGQGSEFVVRLPVLSK
                     PKDLATPAAIVAPQSAPGRKILIVDDNRDSADSLAMLLEITGNQTFLAHDGDEAIAAV
                     EKHRPEVVLLDIGLPKLSGHEVCRRVRAQPWGKDIVVLA"
     gene            complement(2991..3482)
                     /locus_tag="VFV61_06670"
     CDS             complement(2991..3482)
                     /locus_tag="VFV61_06670"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX4900200.1"
                     /translation="MTIKSVHYVMSLLVTLATVVCSVAAPQQSQRIKNERELTALVKQ
                     MAEAQSKFDPATLDKIYTSDFIEISPVGEVDPRDKVLGYYKPEAKPPADMTPTVTSDE
                     FLIRTYSNFAIVIARLTYAVGNAPAARPPFSIRVTLVCRKEKGGWKIASSQYTGIRPP
                     RPQ"
     gene            3636..4526
                     /locus_tag="VFV61_06675"
     CDS             3636..4526
                     /locus_tag="VFV61_06675"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX4900201.1"
                     /translation="MTPRPFFALRAPIILLAAVITTAAQVPNPAPAAAATKSEPRTFA
                     LRSLNDGSAADDIVDIAVYRNALRLEPNDAGHYNNLGYAYARQQNYEEAVRTLEQAIS
                     LDPHLAAAHYNLGIVYDHLGQLPLSLRATRQAVASDPQHMPARVHLCELHLELRQADE
                     AVACYEKLLKAAPPDARSRSNYGSALRRARRFQDALVILEKTIELFPASAEAYNQIGV
                     VLFETGRYDKALVALKQALELAPNLDEPRFNLALAELANRNKTAALEHYGVLKRSNAA
                     LAQQLHEVIYRKHLLTVDRK"
BASE COUNT         1064 a         1346 c         1282 g         1052 t
ORIGIN      
        1 gccaatacca caatatcctt gccccagggt tgcgcgcgga cgcgccggca gacttcatgt
       61 ccgcttagct tcggcaagcc aatatccagt agcactactt ccggccggtg cttttccacc
      121 gcggcgattg cttcgtcacc atcgtgggcg aggaaagttt gattgcccgt gatctccaac
      181 aacatggcga gtgagtcggc agagtctcgg ttatcgtcaa caatcaaaat ttttcgcccc
      241 ggtgccgact gaggagcaac gatcgcagcc ggcgtcgcca ggtcttttgg tttactgagg
      301 acgggcaagc gcaccacaaa ctcgctccct tgtccttcgc ccgcactctt cgcttccacc
      361 gacccgccgt gcatttcaac caaacgtttg accagggtga gcccgattcc cagaccgccc
      421 tgtgaacgtt ccggcgagcg atcgacttgc gtgaacatgt caaagatgct ggccagtttg
      481 tcttgcggta tgccggcgcc agtatcttta actgtcacca ctacctcgct gtcttcccgc
      541 tttgccccga cccagatggt tccgcccggc ttcgtgtacc ggcaactgtt actgagcagg
      601 ttgccaaaca cctgggccag acgggccggg tcggcattca gatagatcgg ctcggcgggc
      661 agggccaggc gcagatcatg gttcgctgaa tcgagcaacg gccgcgccac ttcgacagcc
      721 tggtggatta ccgaagctag ttcaacttca ctttgacgca actcgagacg atcgtgcgtg
      781 atgcgattca ggtccagcag atcgtcgacg aggcgcacca tttgtcccag ctgccgctcg
      841 atggtgtcgc gcgcgcgccc tagcgtttca ccatcaccat cggcgcgctt caaaacttcc
      901 aacatgttga ccagtggtgc cagcggattc ctcaactcat gcgcgagagt cgccaggaat
      961 tcgtttttgc gttggtcggc taaggacaga tccgctgcga gtcttttcag gtcatcggcg
     1021 agtcgccgtc gttccgtgac gtcctggctg acggtcgcaa aaccgattgg ctcaccgttg
     1081 gcatcactca acttcaacac cttgtatgcc atccagcggg cagtgcctgt cttgaagtgg
     1141 cgaaagcgga tttcgacttc gccgtgaccc tgctccatga ccgcagggaa aaactgattc
     1201 atgatccggt cttgatcttc agggaagaaa aaatccgcaa ctggcgtgtt gcgggcctct
     1261 tcgatgctat ccagaccgac catttccagg cccgcacggt tgacgaagaa agggacacca
     1321 tccagatcgc acatgccgat gaagtcagtg ctgctctcaa cgagcgtggc aaacttttcg
     1381 cgatcagctt cgacgcgttt gcgatcggtt atatcgcggg cgatcttcga cgctccgacg
     1441 acattgccgg cttcatcctt gattggcgaa atggtgagcg agacgaggat gcgctgaccg
     1501 tcgctacgca cgcgttcggt ttcaaaatgt tcgatccgtt gtccggcttt taacctcgcc
     1561 agaatctcgt cttcctcgga gatgcgttct gccggaataa caagcgagat gtgttggccc
     1621 acggcctcag cggcggaata accaaagagc cgttccgcgg ccgcattcca gctttgaatt
     1681 acacccgcga gcgattttcc aatgatggcg tcgtttgacg attctacgat tgacgccagc
     1741 aggcgagccg tgtgcagttg attcgccttc tcgcgctcta ccagacgctg ggcactaacg
     1801 tcgcggaaga tcagtacgca cccggacacg tgtccctgct cgtcgcgaat cggcgcggca
     1861 ctgtcatcga ttggacactc gctaccatct tttttgatga gtaaggtgtg gttcgcaagg
     1921 ccgactacga ttccctgatg caaagctttg gttgccgggt tctccactgg cagccgcgtt
     1981 gcctcgttga caatatggaa cactttatcg agtggttggc ccagtgcgtc tgcgagcgcc
     2041 cagccggtta ccgactcggc caccctattc aggtaagtaa tgtgccccgc gttgtcggtg
     2101 gtgatcacag catcgccaat actcttgagg gtgacgctga gtatttcgcg ccgctcggtg
     2161 gcgcgttgct gagcgaatcg cgccgactcg ccgaaggcaa tgatcagtgc gcaagtgaag
     2221 agataggcaa cgagcccgac cacatctgcg agatgattaa gatccagttt gccacgaggt
     2281 tcgatgaata agtaatgaca ggcaagatag ccgaggattg taactacgat ggcgggccga
     2341 taaccgccca accagactgc ggcggcgatg gcaccgaaga gggtgacgag cggtaaggca
     2401 tctcccatca gcgggtcgag cagccaacga aggaggacgg ccactcccag tgcgacgatt
     2461 cctaacgcgt aagttttgat gaaactccgc acgctttccc cttgaagcat gtttgctgag
     2521 attgtcagac ggacgtttac ctggattcta tccttgaaga tagagacgtt caatgtgtgg
     2581 attcaacttc gaagtgcaac agaggaagaa taggtacggg agcgggttcg gccatgatcg
     2641 gaatggggtt cgattagata ccgcacagca acacgcaggc agcagaatca acgatctcac
     2701 cgacaatctc gccatcgacg ctctttgcga gcactgcagc tttcaatgtg actttggtat
     2761 tgaggcatcc gcggcggagg gctgttcacc ggttaaagca caagatctaa acgctgacag
     2821 cgttcgctaa tttcagccca gggttgggct ttggcaaccc tgggtcaacg atcccaaaaa
     2881 tcaattcgca accctgacag gattgcgact aacctcttct cccctacaaa acccagggtt
     2941 tcaaagcaaa ccctgggctg gaattagcca acgcattcag cgttagacta ttactgcggc
     3001 cgtggcggtc taatcccggt gtactggctc gaagcgatct tccaaccgcc tttttcctta
     3061 cgacaaacaa gcgtcactct gatactgaac ggcgggcggg ctgcgggtgc atttcccact
     3121 gcataggtaa gacgggcgat gacaattgcg aagttactgt aggtcctaat caagaattca
     3181 tcgcttgtca cagtcggagt catgtcggcc ggaggcttgg cttccggctt gtagtaccca
     3241 agcaccttgt ctctcgggtc aacctctccc accggagaaa tctcaatgaa gtcggaggtg
     3301 tagatcttgt ccagcgtcgc tggatcgaac ttcgattgtg cctcagccat ttgtttgacc
     3361 aaagccgtca actcccgctc atttttgatc cgttgagatt gttgcggagc ggcgacggag
     3421 cacaccactg tcgccagagt gacgagcagg gacattacgt agtgaacaga tttgatagtc
     3481 ataagaaatc ctaactttgg ttgaatattt cagttatttg gagtaaagga gcgtagcaaa
     3541 aataccagga atcttcagcc cgggtcttta aggttcccaa aagactttct ttagcttgag
     3601 tcaaacaaac gttcgcctgg agaggagata gagctatgac tccccgacct ttctttgctc
     3661 tacgcgcacc aataatcctg ctcgctgccg taataacaac ggcagcgcaa gttccgaatc
     3721 cagctcccgc agctgccgca acaaagagtg aaccgcgcac tttcgcgctg cgaagcttaa
     3781 acgatggcag cgcggctgat gacattgtag acattgcagt ctatcgcaat gcattgcgtc
     3841 tggagccgaa cgacgccgga cactacaaca atctcggcta cgcttacgcc cgccagcaaa
     3901 actacgaaga agccgtccgc acgttggagc aagcgatcag tttggatcca catctggctg
     3961 ccgcccacta caatctgggc atagtttatg accacctggg ccagttgcct ttgtccctcc
     4021 gggccacacg tcaggcggtc gcttccgatc cgcaacacat gccagcccgg gttcacttat
     4081 gcgaactcca tctggagctt aggcaagcgg atgaagcggt cgcttgttat gagaagttac
     4141 tgaaggcggc gccgccagat gccaggtcac gcagtaacta cggttctgct ctgaggcggg
     4201 cgcggaggtt tcaggacgca ttagtgatcc tggaaaaaac catagagctt ttcccggcta
     4261 gtgcggaggc ttacaaccag ataggcgtag tcctgtttga gaccgggcgg tacgacaagg
     4321 ctctggtcgc gctcaaacaa gctctggagc tcgcaccgaa cctggatgaa ccacgtttca
     4381 atcttgcgct cgccgaactt gccaacagaa acaaaaccgc ggcgctcgaa cactatggag
     4441 ttttgaaacg cagcaacgct gctctggcgc agcagcttca tgaggtgatt tacagaaaac
     4501 acttgctgac ggtggaccgg aaataggatc gccaacgtgc tcgctccaac gctgagggcg
     4561 tttgctaatt gcaggtctgg acggcacacg ttgcgcaacc ctttcagggt tgcgacgaaa
     4621 caaaatgtgc gtcttgtacc cagggtttca aagcaaaccc tgggctaaaa ttagccaacg
     4681 ctttcagcgt taatcatgcg ccgggacaac ccggacgtac tccggtcttc accgccggca
     4741 gaac
//
LOCUS       DASUEX010000398         3117 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_747379, whole genome shotgun sequence.
ACCESSION   DASUEX010000398 DASUEX010000000
VERSION     DASUEX010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3117
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_747379"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            148..285
                     /locus_tag="VFV61_06680"
     CDS             148..285
                     /locus_tag="VFV61_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900202.1"
                     /translation="MAEILREQIAGSGKAEIKQNLTKPLPASRRTFALPASDARTKEE
                     D"
     gene            288..2186
                     /locus_tag="VFV61_06685"
     CDS             288..2186
                     /locus_tag="VFV61_06685"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEX4900203.1"
                     /translation="MNALKVIITSSCLMMALFQSSPGQQVPAPPSVVAAEERGTREQD
                     GLQGPVRRVRVETAKIVVKSDKMVEGPRVVRGIATYDALGKKIDAVDYPGESSTVPGK
                     EQYRYDDKGNIVEMVVLGSDGSILGKEAYNYEFDPMGNWTKMNTAVAVYENGKVTFEP
                     TEVTYRTISYYYNQAIEKLSNTAAKSKGAAKPATPTPGSEALNPLKTVAKPIATNHPV
                     AEKVAAIEKDPETRTTPAPAAPTQNNEAIGNAATPAKEAGTATAEPAKPNVIKVQESV
                     LRSAAMDLPQPEYPQAALLVRATGTVEVQLLVNEKGVVTNARAQSGNPLLIPAAETAA
                     LKARFSPSKLSAEPTITFGVITYNFTPPEAPSAAPESKPISGSQSFAAQKQTVTPTVA
                     PNQAAEKNASLATRPATFTEAQPKPSLESEASHYGRGVAFFNAGHYEEAAMAFNQAIQ
                     ADANDANAYVKLALSYSGMHKDKEAIAGYKMAKQIQSSVFDAYAYFNWGRSYLALDKT
                     SDAISAFKQALSLMRAEAIGLEPKTSASLPTPEQVHHHLGVAYINARRFTDAIKEFKQ
                     VVALNPANAEAHYALAVSYLNTDDHKAAQDENKILASLDAEMAKKLAAVLADASSRFG
                     CRNVACRR"
     gene            2274..3116
                     /locus_tag="VFV61_06690"
     CDS             2274..3116
                     /locus_tag="VFV61_06690"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEX4900204.1"
                     /translation="MTIAPDTLTLFERDGFLILKDFVTTTACDELRVRAEQLVQDFDP
                     AGVISIFTTHEQSRVADDYFLESSDKIRFFFEEDAFLPDGTLKQSKERSINKIGHALH
                     AVDPVFRAFSHSSKIKQLVAELAIQDPQLAQSMYIFKQPKIGGEVACHQDSTFLYTEP
                     ANIAGLWLALEDATINNGCLWAIPGGHRVGLKSRWVRAPEGGMYFNVFDSAPWPEEQL
                     QPLEVSQGTLIVLHGLSPHKSLANRSNKSRHAYTLHVISGKSHYAADNWLQPRTASQG
                     FDPL"
BASE COUNT          789 a          845 c          817 g          666 t
ORIGIN      
        1 ttactccctt aattgttgtt cctgggaggc cagttcctga ctcagacttc aggaaaattc
       61 aaaccgcaga ttttacatac cgtcggcctc gcgtcctatt ggatcaacag tccgcagatt
      121 agatctcgag atttttccgc cagctacatg gctgaaatct tgcgcgaaca gattgccggc
      181 tcaggtaaag ctgagattaa gcagaacctg actaaaccac tgccggcgag cagacgaacc
      241 tttgcattgc ctgcaagtga cgcgcgaacc aaagaggagg attaacgatg aacgctctaa
      301 aagtcattat cacttcttct tgcctcatga tggcgctttt tcagtccagt cccggacaac
      361 aagtgccggc gccaccttca gtggtcgcgg ccgaggagcg gggcacgaga gagcaggatg
      421 gtttacaggg acccgtgcga cgtgtgcgcg tggagactgc aaagattgtg gtgaagagcg
      481 acaagatggt tgagggacca cgcgtcgtgc gcggaatcgc aacttacgat gccctgggta
      541 aaaagattga tgcggttgat taccccgggg aaagcagtac tgttccgggg aaagaacaat
      601 atcgctacga cgacaaggga aacatcgtgg agatggtcgt cctgggcagc gatggttcca
      661 ttctcggcaa agaagcgtac aactacgaat ttgatccaat ggggaattgg acgaagatga
      721 atacggctgt ggccgtttac gaaaatggga aagtgacgtt tgaacctacc gaggtgacct
      781 atcgcacgat tagttattac tacaatcagg caattgaaaa actcagtaat actgccgcca
      841 aatcgaaggg cgcggcgaag cctgccactc ccacgcctgg ttcagaagcc ctcaacccgt
      901 taaaaactgt tgccaagcca atcgcgacga atcacccagt ggcagaaaaa gttgctgcga
      961 ttgaaaaaga ccctgaaacc aggacgactc cagctccggc cgcaccgact caaaacaacg
     1021 aggcgattgg aaatgcggcg acaccagcta aggaagccgg caccgctacc gccgagcccg
     1081 cgaaacctaa tgtgatcaag gttcaagaaa gcgttttgcg cagcgcggca atggacctgc
     1141 cgcaaccgga ataccctcaa gcagcgctgc tggttcgggc taccggcaca gtagaagtac
     1201 agctattagt gaatgaaaaa ggtgtcgtta caaacgcgcg cgcccaaagc ggcaaccctt
     1261 tgttgattcc ggccgcggaa acggcagccc tgaaggcgcg tttctcgccg tcaaaacttt
     1321 ctgcggaacc gacgataacc ttcggcgtca ttacctacaa tttcactcct cccgaagcgc
     1381 ccagtgcagc gcccgagagc aaacccatta gcgggagcca gtctttcgca gcacagaagc
     1441 aaacggttac tcctactgtt gcgcctaatc aagctgcgga gaagaatgcc tcgctggcaa
     1501 ccagaccagc aaccttcacc gaagctcagc caaaaccaag tcttgaatca gaagcgtcac
     1561 attacggcag gggcgtcgcc ttctttaatg cgggtcatta cgaggaagct gcaatggctt
     1621 tcaatcaggc cattcaagcg gatgccaacg acgcgaatgc ttacgtgaaa cttgccctgt
     1681 cctattccgg aatgcacaag gacaaagagg cgattgccgg gtacaaaatg gcgaagcaga
     1741 tccagtcaag cgtcttcgat gcttatgcct acttcaattg gggcagatct tatcttgcgc
     1801 ttgataagac ttccgacgcg atttcagcat tcaagcaggc gctttccctg atgagagccg
     1861 aagcgatagg tcttgagccc aaaaccagcg ccagcctgcc cacacccgaa caagtgcatc
     1921 accacctggg agtcgcctac attaacgccc gtcgttttac agatgcgatt aaggaattca
     1981 agcaagtggt cgccctgaat ccggcaaatg cggaggcgca ctacgctctg gcagtttctt
     2041 atctgaatac cgacgatcac aaagcggcgc aagatgagaa taagattctc gcttcgcttg
     2101 acgccgaaat ggcgaagaag ctcgccgctg ttctcgccga cgcctcatca cgttttggtt
     2161 gtcgcaacgt tgcgtgccgg agatagacgg tcggcagttc tcaaaagcgt gacgcgcagc
     2221 tcaccatttc tgcgcgtcac gctccgataa cttggtggta atctctttcc accatgacga
     2281 ttgcgccgga cactctaact ctcttcgagc gtgacgggtt tctaatcctg aaagacttcg
     2341 ttaccacgac cgcttgcgat gaattgcggg tgcgtgccga gcaacttgtt caagattttg
     2401 atcccgccgg cgttatctct atcttcacaa ctcatgagca gagtcgagtg gcggacgact
     2461 actttctcga atccagcgat aagattcgtt tcttctttga ggaggatgcg tttcttccag
     2521 atggaacctt aaaacaaagc aaagagcgct cgatcaataa gatcggacac gcgctgcacg
     2581 cggtcgatcc tgtcttccgg gcgttttcac attcttccaa aatcaagcag ctagtggcgg
     2641 aacttgcgat tcaagatccg cagctcgcgc agtcgatgta catctttaag cagccaaaga
     2701 ttggcggtga agtggcctgc catcaggaca gcacctttct ttacaccgag ccagctaata
     2761 tcgcgggcct ttggctggca ctggaggacg ctactatcaa taacggttgt ttgtgggcga
     2821 ttcccggagg tcaccgggta ggtctcaaat cccgctgggt gcgcgcacca gagggcggca
     2881 tgtattttaa tgttttcgat agcgcgccgt ggccggaaga gcaattgcag cctcttgaag
     2941 tgtcgcaggg taccttgatt gtcctacacg gcctgtcgcc tcataagagc ctggccaacc
     3001 ggtcaaataa gtcgcgtcat gcctacacgc tgcacgtaat tagtggcaaa agtcattacg
     3061 ccgccgacaa ctggttgcag ccgcgaaccg catcccaggg ctttgaccct ttgtgaa
//
LOCUS       DASUEX010000399         2632 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_170600, whole genome shotgun sequence.
ACCESSION   DASUEX010000399 DASUEX010000000
VERSION     DASUEX010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2632
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170600"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1876
                     /locus_tag="VFV61_06695"
     CDS             <1..1876
                     /locus_tag="VFV61_06695"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX4900205.1"
                     /translation="FGCILFEAATGKKPFEGDSIVKSLHSVIYEPAPQIKDLNPAAPA
                     DLQRIIRRCLAKDKDERYQTIKDVAIELKELRRELETSGDFDTTVPPPGSSVGTRATE
                     SGSAIEATTDRSASTSPTVSSAEYVTQGLKRHKLGVAVALAILIAGIALSAYFLRASG
                     GEGAIHSIAVLPFQNKSSDADTEYLSDGLAESLIFRLSQLPNLKVSPTSSVFRYKGKD
                     TDIAQIGKELEVDAVMSGRVSQRGNDLTISVELVDARTKKLIWAEQYDRKMSDLLATQ
                     REIATTIAQKLELKLSGNDDKGITKRYTDSNEAYQLYLRGRYSFAKRTKDEMLRAIEY
                     FQQAIKLDPKFALAYARIAETYGSMPAYPYLSPKEAFPQAKAAAQKALEIDPTLAEAH
                     TFLAYSLVIYDWNWAEAERSFKRAIELDPNNSAAHFRYGQIYLAPTGRLDEGIAEIKR
                     GLELEPLDINMGATLAWAYLVAGQNDRALEQAKKTYDLEPTHPIGRWILSQAYIFNGS
                     YQEAISLNEQWLQTDPTNQFPIRDAGIAYAKTGRRDKAEEMIARFREIARTQYVPTCR
                     IAGIYVALDDKDKVFGELNKSFQERDWELYRLNADTYWNPLREDPRFKEMLKRLNLPE
                     "
     gene            2018..2587
                     /locus_tag="VFV61_06700"
     CDS             2018..2587
                     /locus_tag="VFV61_06700"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX4900206.1"
                     /translation="MAKPSKFAHVVYSTRRFDEMIDWYQNVFEAKVVYQNPVFAFLTY
                     DDEHHRFAFANLSALTPDSVANETRDNAGVNHVAYTYANLGDLLATYERLKQSGITPY
                     WRIHHGITLSLYYQDPDGNRMEFQVDCGTVEEANAYMQTDAFAANPAGVEIDPEILLK
                     QYRSGVPEQQLLVMPEGPMSPIPREHGMS"
BASE COUNT          681 a          709 c          693 g          549 t
ORIGIN      
        1 ctttggctgc atcctgttcg aggcggccac cggcaagaag ccattcgaag gtgattcaat
       61 cgtcaagtcc ttgcacagtg tgatctatga acccgcgccg caaatcaagg atttgaatcc
      121 ggcagctccc gcggacttgc agcgtatcat tcgtcgctgt ttggcaaagg acaaagacga
      181 gcgctaccag acaatcaaag acgtcgcgat cgagttgaaa gaactacgtc gcgagttgga
      241 aaccagtgga gactttgaca caactgtgcc gccgcccggc agcagcgttg gcacgcgcgc
      301 cactgagagt ggctcagcta tcgaagccac cacagacaga tcagcatcaa cgtcgcccac
      361 cgtctcaagt gctgaatacg taacgcaagg gctcaagcgg cacaaactcg gtgtcgcggt
      421 cgcgctggcg atattgattg ccggcattgc gctctcggct tacttcctgc gcgcgagcgg
      481 cggcgaagga gccattcact caattgcggt gttgcccttc cagaacaaaa gctcagacgc
      541 agacacggaa tatctgtccg acggcctggc ggagtcactt atcttccgtc tttcgcaact
      601 gccgaatctg aaagtcagtc caacgagttc cgtttttagg tacaaaggga aggacacaga
      661 catagctcag atcggcaaag agctcgaggt tgatgcggtc atgtccggtc gtgtctcgca
      721 acgaggcaac gatttgacga ttagtgtcga actggttgac gcgaggacaa aaaaactaat
      781 ctgggccgaa caatatgacc gcaagatgtc cgatctgctg gcaacccagc gcgaaattgc
      841 taccaccatc gcccaaaaac tcgaactaaa actgtcaggc aacgacgaca aaggcattac
      901 caaacgctac acggatagta acgaggcgta tcagctttac ttgagaggcc gctacagctt
      961 tgccaagcgc accaaggacg aaatgctgcg cgctatcgaa tacttccagc aggcgatcaa
     1021 actcgatccg aagtttgcgt tggcgtatgc gcgaatcgcc gaaacatacg ggagtatgcc
     1081 ggcttatccg tacctctcgc caaaggaagc ttttcctcag gccaaggcgg cggcgcaaaa
     1141 agctcttgag atcgacccaa ctctggctga ggcccatacg tttttggcgt actccctggt
     1201 gatttacgat tggaattggg ccgaagccga gcgctccttc aaacgcgcga tcgaacttga
     1261 tccgaataat tccgccgcac attttcgtta cgggcagata tatcttgctc cgaccggtcg
     1321 ccttgacgaa ggcattgccg aaattaagcg gggtctggag ctggaaccgc ttgatatcaa
     1381 catgggagca actttggcgt gggcttatct tgtggccggc cagaacgaca gggcgttgga
     1441 gcaagccaag aaaacatacg atctcgaacc cactcacccg ataggacgct ggatcttaag
     1501 ccaggcttat atctttaacg gaagctacca ggaggcaatc tcattaaacg aacaatggct
     1561 gcaaaccgat cctacaaacc aatttccgat tcgagatgcc ggaatcgcat acgcgaaaac
     1621 cggacgaagg gataaggcgg aagagatgat cgctaggttc cgggaaatcg ctagaacgca
     1681 atatgtaccg acgtgccgga tcgcgggtat ctacgtcgcc ctcgatgaca aggacaaggt
     1741 gttcggggag ctcaataaat cgtttcagga acgggattgg gaattgtatc gattgaacgc
     1801 tgacacttat tggaatccgc tccgcgaaga tccgcgattc aaggaaatgc tgaagcgcct
     1861 gaatctgccg gaatgagttc ggagcagcag ttcagcgcct tacagctcag cgccaatgaa
     1921 cattacatga ccgggagcac cgaccgacaa ggcggcggaa tcaccatccg atccaaccat
     1981 cattccactc atgatttaga tcgaaaggag tcgacatatg gcaaaacctt cgaagttcgc
     2041 gcacgtcgtt tatagcacca gacgctttga tgaaatgatt gactggtatc aaaacgtctt
     2101 cgaggcgaaa gtggtctatc aaaatccggt gttcgctttt cttacctacg acgacgaaca
     2161 tcatcgtttc gcgttcgcca atttgtcggc gctcactcct gacagcgttg cgaacgagac
     2221 gcgggacaac gctggtgtga atcacgttgc gtacacctac gccaacctcg gtgacctgct
     2281 tgctacttac gagcgtctca aacagagtgg gattaccccc tattggcgca ttcatcatgg
     2341 gattacactt tcgctttatt accaggatcc cgacggcaat cgaatggaat ttcaagtgga
     2401 ctgtggtacg gtcgaagagg ccaacgccta catgcagact gacgccttcg ctgcgaatcc
     2461 ggccggggta gaaattgatc cggaaatcct gctcaaacag taccgcagcg gtgtgccgga
     2521 gcaacaactc ctggtgatgc ctgaaggtcc gatgtctccc atcccgcgcg agcacggtat
     2581 gtcctgattc tgattccatt cagggaaatg ctgaacacct gaacctgccg ga
//
LOCUS       DASUEX010000400         3404 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1202984, whole genome shotgun sequence.
ACCESSION   DASUEX010000400 DASUEX010000000
VERSION     DASUEX010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3404
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1202984"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(203..292)
                     /locus_tag="VFV61_06705"
     tRNA            complement(203..292)
                     /locus_tag="VFV61_06705"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(256..258),aa:Ser,seq:gga)
     gene            complement(440..529)
                     /locus_tag="VFV61_06710"
     tRNA            complement(440..529)
                     /locus_tag="VFV61_06710"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(493..495),aa:Ser,seq:cga)
     gene            complement(515..1012)
                     /gene="tadA"
                     /locus_tag="VFV61_06715"
     CDS             complement(515..1012)
                     /gene="tadA"
                     /locus_tag="VFV61_06715"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: protein motif:HMM:NF008113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HEX4900207.1"
                     /translation="MTAATKMDLDEQWMSRAIAAAREAEALGEIPVGACVVSENKLLA
                     VSGNRTRTDGDPTAHAEIVALRAAAKVLGNYRLTDVVVYATIEPCAMCAGALIQARVK
                     RLVYGARDERAGAVESRFRICDSDFLNHRLELTTGVLEAECRELVQNFFRTRRCEIGQ
                     RGEVA"
     gene            1221..2228
                     /locus_tag="VFV61_06720"
     CDS             1221..2228
                     /locus_tag="VFV61_06720"
                     /inference="COORDINATES: protein motif:HMM:NF021539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2270 domain-containing protein"
                     /protein_id="HEX4900208.1"
                     /translation="MTIEANQRGSGPLTTDRVQSRMRGAPSASELPGGDKTSQTPAVL
                     MRRMSPVEFNTAMIHFYRGEVQRSNTWRNRLDTTTNWAVLTAGATLSFVFSSPSNAHF
                     VIPINSILVAIFLLMEARRYRYYEIWSSRVRVLETGYFAQLLAPENALADEAWAQHLA
                     SDLITPHFTISEWEAVGRRLRRNYLWIFALLALSWNLKVYIHPLPARDFHAFLDRADV
                     GIVPGWIVFVVGVVFNAAIAIFAIGTVRLREATGEVLPQHEFSLRPLRTMKKWTRAAT
                     GTGRTTMRARRARQRVRSGALTGEFKKIDISETPDRESHRIDRSESPEREVPAESLTR
                     G"
     gene            2337..>3404
                     /locus_tag="VFV61_06725"
     CDS             2337..>3404
                     /locus_tag="VFV61_06725"
                     /inference="COORDINATES: protein motif:HMM:NF012447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HEX4900209.1"
                     /translation="MFELSGQKLSLQQIVDVAEGREHVRLSSAARARCDASRAVVERI
                     VAEGRTVYGVNTGFGRLSDVRIEPNQLRDLQVNLVRSHACGLGSSLSESEARAMLLLR
                     ANVLACGYSGGRALLIDTLLAMLDHGVTPVIPEKGSVGASGDLSPLAHLALVVIGEGE
                     AFYKGLRMPGAEALAKAALKPVSLEVKEGLALLNGTQAMAAVGSLALRRATRLTLVAD
                     VAGAMSLEALRGTPVAFDERIQQARPHPGQIAVAANLRALMSDSEIRTSHLENDPRVQ
                     DAYSLRCMPQVHGAVRGALDHVREIVETETGSATDNPLVFAETGEVLSGGNFHGAPLA
                     MAFDYAAIALTDLMSISERRID"
BASE COUNT          752 a          925 c          960 g          767 t
ORIGIN      
        1 gccacggccg tgatagccag gacaatggtt acgcgcggaa ggctgaactt gatcatgttt
       61 gctccttaat tcaaggaaaa agtaaaaagg caaaaaatag gagcggccgg gcgatctatc
      121 ggtattcgat ggctgtcccc tgcacaataa ttcggtgttc ttacttttac ctttttcctt
      181 tttacttttt ccttcgcggt tatggcggag agggtgggat tcgaacccac ggaacggttc
      241 cccgttcaca gcatttccag tgctgccaat tcaaccactc ttgcacctct ccagggattg
      301 ccgattgcca attggcaatt gccgattggc aagttccgat taacgagtgc tgatcggcaa
      361 ctatatgtct gacatcaagt ttcgagcttt ggataaacta caccaatcag caatcgccaa
      421 tcggcaatca tcaatagtct ggcggagagg gtgggatttg aacccacggt ggagtttccc
      481 ccactccggt tttcgagacc ggtgcactaa gccactatgc gacctctccg cgttgcccaa
      541 tctcacatcg ccgagtgcgg aagaagttct gcaccaattc acggcactcg gcctcaagca
      601 cgccagtagt gagttccagg cgatgattca gaaagtccga atcgcagatg cgaaatcgcg
      661 attcaaccgc tcccgcccgt tcatcgcgcg caccataaac cagccgcttt acgcgcgctt
      721 gaatgagcgc gccggcacac atagcacagg gctcaatcgt ggcataaacg actacgtctg
      781 tgagtcggta attgccgagc acctttgccg cagctcgcaa agcaacgatc tcggcgtgtg
      841 cggtcggatc accatccgtg cgcgttctgt ttccggaaac cgccagcaat ttgttttcgc
      901 tgacaacgca agcgccgact ggaatctcgc cgagcgcttc agcctcacgc gccgccgcga
      961 ttgcccgcga catccactgc tcatccagat ccattttcgt agcagcagtc aacaatcaaa
     1021 aaaaattagt caaacagcga aaaatcaaac tataggtgtc tggctcgaac actgtcaagc
     1081 aggccaaaat tgaacaactt caacgcttgc agcctccaga aacgctccaa cttaactacg
     1141 atcgaatctg aggcgcgtgc tattatcgga gtgcggagtg gatgtcctaa caccctaccg
     1201 gaccagattc gttccattcg atgaccattg aagcaaatca gagaggaagt ggcccgttaa
     1261 cgaccgatcg ggtgcagagt cgcatgcgtg gcgcgccctc agcgtcggaa ttgcccggcg
     1321 gcgacaagac ttcacagacg cccgcggtgc taatgcggcg catgtcgcca gttgaattca
     1381 acaccgcgat gattcacttc taccgcgggg aagtgcagcg ctcaaacacc tggcgcaatc
     1441 gactcgacac cacaactaac tgggcggtgc tcaccgcagg cgctactttg tcctttgtat
     1501 tcagttcgcc cagcaacgct catttcgtaa ttccgattaa ctcgatcctg gtcgcgatct
     1561 ttttgttgat ggaagcgcgt cgttatcgct actacgaaat ctggtcgagt cgtgtgcgcg
     1621 tgctcgagac aggatatttt gcgcaactgt tggcgccgga aaatgcgttg gccgatgagg
     1681 cctgggcgca gcacctggcg tcggatttga taacgccgca ctttacgatt agcgaatggg
     1741 aagctgtcgg caggcgcctg cggcgcaact acctttggat ttttgcgctg ctcgccctca
     1801 gttggaatct caaggtctat attcatcctt tgccggcgcg cgacttccac gctttcctgg
     1861 accgtgctga tgtgggaatt gttcccgggt ggatcgtgtt tgtagtcggc gtcgtcttca
     1921 acgccgcgat tgcgatattt gcaattggca cagtgcgttt gcgcgaagca accggcgaag
     1981 tcttgccgca acatgagttt tccttaaggc cgctgcgtac gatgaagaaa tggacacgag
     2041 cggcaaccgg caccggtcgg acaacaatgc gcgcgcgtcg cgcacgccaa cgggttcgca
     2101 gtggcgcctt gacgggtgag ttcaagaaga tcgatatctc ggagactcca gatcgcgagt
     2161 cacacaggat cgaccgtagt gaatcgcctg agcgagaagt gccggcagaa tcattaacca
     2221 gaggctagct cgtccggaaa ttgccgggga gactgacttc caggcagagc ttccagtgat
     2281 tgtgagttga aagctcacct gtaagtcggt tactggatcg tgcttcgtat gatcttatgt
     2341 tcgagttaag cggtcagaaa ctttcccttc aacaaattgt cgacgtcgct gaaggtcggg
     2401 agcatgtgag gctctcgagc gcggcccggg cacgttgtga cgcttcgcgg gcagttgtag
     2461 agagaatagt cgctgaaggt cgcaccgttt atggagtcaa taccggcttc ggccgccttt
     2521 ccgatgttcg catcgaacca aatcaactcc gtgatctgca agtgaatctg gtgcgcagtc
     2581 acgcgtgtgg tctcggctcg tcgctgtcgg agtctgaggc acgcgcaatg ttactgttgc
     2641 gggcgaacgt gctcgcttgt ggctacagtg gcggtcgggc gcttttgatc gacacgcttc
     2701 ttgccatgct cgaccatggc gtgacgcctg tcattccgga aaagggatcg gtcggagcaa
     2761 gtggtgattt atctcccctc gcgcatctgg cgcttgtggt aatcggtgag ggtgaagctt
     2821 tctacaaagg cctgcgaatg ccaggcgccg aagcgttggc gaaagcggct ctcaaaccgg
     2881 tgagcctgga agtgaaggaa ggtctggcgc tgctcaacgg tacgcaggca atggcggcgg
     2941 tgggcagtct tgctttaagg cgggcgacac ggttgacgtt ggttgcagac gttgccggcg
     3001 cgatgagcct ggaagcacta cgaggcactc ccgtggcttt tgacgaacga attcagcagg
     3061 caaggcctca tccgggacaa attgccgttg ccgccaactt gcgcgcattg atgagcgaca
     3121 gcgagattcg cacgtcacat ctcgaaaacg atccgcgtgt gcaagacgct tacagtttgc
     3181 gctgcatgcc gcaggttcac ggggctgtca ggggagcttt agatcatgtg cgtgaaatcg
     3241 tggaaactga aaccgggagc gcaacggaca atcctcttgt gtttgccgaa accggtgaag
     3301 tcttgtcggg aggaaacttt cacggtgccc ccctggcaat ggcgttcgac tacgcagcca
     3361 ttgcgctcac tgacctgatg agcattagcg aacgacggat cgac
//
LOCUS       DASUEX010000401         2347 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1653354, whole genome shotgun sequence.
ACCESSION   DASUEX010000401 DASUEX010000000
VERSION     DASUEX010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2347
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1653354"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..484)
                     /locus_tag="VFV61_06730"
     CDS             complement(95..484)
                     /locus_tag="VFV61_06730"
                     /inference="COORDINATES: protein motif:HMM:NF022963.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3224 domain-containing protein"
                     /protein_id="HEX4900210.1"
                     /translation="MNKASGTFAVKVTPQAPEEGDTSGIGRLLLDKQFEGDLEATSKG
                     QMLAMSSAVEGSAGYVAMEQVTGTLHGKTGAFALQHFGKMTRGTPELNVMVVPDSGTG
                     DLEGLSGRLQIIIADGSHSYEFEYELK"
     gene            complement(503..931)
                     /locus_tag="VFV61_06735"
     CDS             complement(503..931)
                     /locus_tag="VFV61_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900211.1"
                     /translation="MPKLNWNRIIIGGLIATVILFLTDGFFHESVVGADWKAVYAALG
                     AREPQHSAAGMAYFVIFELGRGFISIFIYAFLRQFFKAGPNTAVLAGIVAWLAFSVTG
                     PAQFIPLEFYSQALWVKVAAFQLVTSIVATIAGAAIYKDN"
     gene            complement(994..1275)
                     /locus_tag="VFV61_06740"
     CDS             complement(994..1275)
                     /locus_tag="VFV61_06740"
                     /inference="COORDINATES: protein motif:HMM:NF021552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HEX4900212.1"
                     /translation="MCRNIKMLHNFKPPATDEEIRASSLQFVRKLSGFNKPSKTNELA
                     FNRAVDEVAKAAHELLDSLVTNAPPRDRAVEAAKARARTAERFHQSNAN"
     gene            complement(1302..1655)
                     /locus_tag="VFV61_06745"
     CDS             complement(1302..1655)
                     /locus_tag="VFV61_06745"
                     /inference="COORDINATES: protein motif:HMM:NF013734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900213.1"
                     /translation="MKVAAIKPAITLKEVEKVDIRVGTIEAVEDLENSNKLVRLVVDF
                     GDHKRRILAGLKQEHTNPRELEGKQALFVVNLEPKRMAGEISEGMLFDIGYGDGEVPC
                     LAVPERQMPNGSRAG"
     gene            complement(1667..>2347)
                     /locus_tag="VFV61_06750"
     CDS             complement(1667..>2347)
                     /locus_tag="VFV61_06750"
                     /inference="COORDINATES: protein motif:HMM:NF016021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900214.1"
                     /translation="MAATSSEARRRAFKGFRVDSGNVAELGAWCYRFFEANGLRGLRP
                     NLTGDLDVEKVRAIVDEFPEAAGFGIGTKLSSEVRAVAGVIFKQCVIDSRPTLKASNS
                     LDKTTLPGRLQLFRGIDVRGNYVADVTGLDDEDIEIPGAARVERLLVPFWENGQHAAI
                     PSIEKQKAMVTEQRRRFADIENYPRSLSERLRTLVEELTAQMRVDNSGWQEVVKLPET
                     VPGELVSK"
BASE COUNT          525 a          673 c          595 g          554 t
ORIGIN      
        1 gttatgaaaa cctggctgtt tattttctta gcggctctgt tcctccgcgt ctctgcgggt
       61 tgaatgaaaa ctgggaaatg agattgtgga aaagttactt gagttcatac tcaaattcgt
      121 aagagtgact tccatccgca atgatgatct gcaggcggcc cgagagtcct tccagatcac
      181 cggtgcccga atcgggcacc accatgacat tcaattccgg cgtgccgcgc gtcatcttgc
      241 caaagtgctg caaagcgaac gcgcctgtct taccgtgcaa ggtgccggtc acttgctcca
      301 ttgcaacata accagcagag ccctcaacgg cggagctcat cgccaacatc tggcctttgc
      361 tggtcgcttc aagatcaccc tcaaactgct tatctaacaa cagccggcca atgcctgatg
      421 tgtcgccttc ctctggcgcc tgcggcgtta cctttaccgc aaatgttccg cttgccttgt
      481 tcattcttgt ctcccgtgtt gtctaattgt ccttatagat tgccgcgcca gcgattgtgg
      541 cgacaatgga agtgacaagt tgaaatgccg ccaccttgac ccaaagcgcc tgtgagtaga
      601 actctaaagg tatgaactgc gccggccccg ttacggaaaa agcgagccag gcgacgatgc
      661 ccgccaggac ggcagtgttt ggcccggctt tgaaaaactg ccgcagaaac gcgtagatga
      721 agatcgagat aaacccgcgg cctagttcaa aaatcacgaa atacgccatg ccggcagcgc
      781 tgtgttgtgg ctctcgggcg ccgagagcag cgtagacggc tttccaatct gccccgacca
      841 cagattcatg aaagaagccg tcagttagaa acaaaatcac ggttgctatc aaaccgccga
      901 tgatgattcg gttccagttc agcttgggca cggtttacct ccggtagggg agcgagaggt
      961 tagtaaaacg aaggttggtg gtagcaccga ttatcaattg gcgttgctct ggtgaaatcg
     1021 ttccgccgtg cgagccctgg ctttggctgc ctcaactgcg cgatcgcgcg gcggcgcatt
     1081 agttactagc gaatcgagca gttcgtgggc cgctttggcc acctcgtcca cagcgcgatt
     1141 aaaggcgagc tcgtttgtct tcgagggttt gttaaagcca cttaacttcc gcacaaattg
     1201 caacgacgat gcgcgaatct cttcgtctgt tgccgggggt ttgaagttat gaagcatctt
     1261 aatatttcta cacatgactt attcccttat cacttttgat ttcaacctgc gcgactgccg
     1321 ttcggcattt gccgctcagg aactgccaga caaggcacct cgccatcgcc ataaccaatg
     1381 tcgaacaaca tgccttccga tatttcaccc gccatccgtt tcggctcaag attcacgacg
     1441 aacaaggctt gcttaccttc cagctcgcgc ggattagtgt gttcctgctt caagcccgca
     1501 agaatgcgac gcttatgatc tccaaaatct acgaccagcc gcactagctt attcgagttt
     1561 tcgagatcct ccactgcttc gatggtgccg acgcggatat cgaccttctc aacttctttc
     1621 aaagttattg caggtttgat ggccgcaact ttcatcgatt cagcaactac ttactgacca
     1681 attcgcctgg caccgtctcc ggcaacttaa ccacttcctg ccagccagag ttgtcgacgc
     1741 gcatttgtgc ggtcagctcc tcaaccaatg ttcgcaggcg ttcactcaac gagcggggat
     1801 agttctcgat atcggcaaac cgccggcgct gctcggtgac catcgctttc tgtttctcga
     1861 tcgaaggaat cgcggcatgt tgtccgttct cccagaaggg aaccaaaaga cgctcgactc
     1921 gagcagcacc gggaatttca atgtcttcgt cgtccaggcc ggtcacgtcg gcaacataat
     1981 tgccgcgcac atcaatgcca cggaaaagtt gtaagcgccc gggcaacgta gtcttgtcaa
     2041 gcgagttgga agccttcaac gtcgggcgcg aatcgatcac gcattgtttg aaaatcactc
     2101 ccgccacggc gcgaacctcg ctggagagtt tcgtgccgat gccgaaaccc gcagcttcgg
     2161 gaaactcgtc gacgatggcc cgcaccttct ctacatcgag atcgccggtg agatttggtc
     2221 gcagcccacg caaaccgttg gcttcgaaga atcgatagca ccaggcgccg agttcagcca
     2281 cgttgccgga gtcgacgcgg aatcctttga atgcacggcg acgcgcttcg gaagaagttg
     2341 cggccat
//
LOCUS       DASUEX010000402         3108 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1111979, whole genome shotgun sequence.
ACCESSION   DASUEX010000402 DASUEX010000000
VERSION     DASUEX010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3108
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1111979"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="VFV61_06755"
     CDS             <1..304
                     /locus_tag="VFV61_06755"
                     /inference="COORDINATES: protein motif:HMM:NF024937.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HEX4900215.1"
                     /translation="DFDRGVQVAAFLLGGGLALSAHGTKAGARAAINLSPEPASNIVA
                     SLTEDVVAVGSILLSVFAPFILFAVITIFLIISFLMARKILSAMKAMMLRLRQAFS"
     gene            543..1859
                     /locus_tag="VFV61_06760"
     CDS             543..1859
                     /locus_tag="VFV61_06760"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF004126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HEX4900216.1"
                     /translation="MSSQTLTITDNRTGKQYEVPIENGTIKAMDLRQIKTADDDFGLM
                     TYDPAFMNTASCKSRITFIDGDKGILEYRGYPIDQLAEKSSYLEVAYLLLHGELPNEA
                     ELREWTWNITHHTFINENIKKVIDGFHYNAHPMGMFEATLGALSTFYPDAKDIFNVEC
                     RRKQIYRLIAKVPTIAAFAFRHRIGMQYSYPDNDLSYEGNFLNMMFKTTELQYKPDPV
                     LEHALTVLFILHADHEQNCSTNAMRSIGSAQTDPFSALGGAAAALYGPLHGGANEMVL
                     RMLKEIGSVDKVPDYVKRVKAGEFRLMGFGHRVYKNYDPRARIIKQVAYEVFEVTGKD
                     PLLDIALELERIALEDEYFVKRRLYPNVDFYSGIIYQAMRFPLDMFPVLFAIPRTSGW
                     LAQWVELLEDPDQKIARPRQVYLGQRTRNYVPLANRKVPETQSAGA"
     gene            complement(1877..2854)
                     /locus_tag="VFV61_06765"
     CDS             complement(1877..2854)
                     /locus_tag="VFV61_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900217.1"
                     /translation="MSSQSTLLPDPPVVAESAPSSIRTQPKIPHEQIDQLLEEIEHQQ
                     VRKMMSEAFSALLSLLQDVPALASLNEGPNPADAKVVIERFRREATSLVDLIASLATA
                     TEGLDRSVAETLDGIAFALSHDLKRTFDSELKGLGWKPDNVARGKIAYVSGLLTNCLQ
                     QSVITLAQVFEPGLDGGKLFDNYQARLRESLLLCRDLTDMILFAQACENDIAQNFPKL
                     VTSISSFRDESMQFLMYKDWHEFESLSESLLQRVPESAAVGSPLHSFTCYLETLLGQV
                     KLRAVLVDVFCDFFPDGQSTDADWSEAQNRLAFELYRAQLMSTIERSAI"
BASE COUNT          742 a          825 c          802 g          739 t
ORIGIN      
        1 cgactttgat cgtggagtgc aggtcgcggc cttcttactc ggtggcgggc tggcgctcag
       61 tgctcatgga acgaaggcgg gcgcccgggc ggcgatcaat ttgagtcctg aacctgcttc
      121 gaacatcgtc gccagcttga cggaagatgt tgtcgcagtc gggtcgatac tgctttcggt
      181 ctttgctccc ttcatcctgt ttgcagtaat cacaattttt ctcatcatct ctttcctgat
      241 ggctcgcaaa attctgagtg cgatgaaagc catgatgttg cgactgcggc aggcgtttag
      301 ttgacgcttt ttcgtgtcat ttcgtatgaa tcagtggatt gtccccttga tggtcgagaa
      361 ggccatccac aaagtcacag taaataccac tccctcaacg cgccccggct cattcgtgtg
      421 aaaactgaac tgtgattagg atcacagtca aatacacctc caatttgatt cgtaagcacg
      481 gcggagttat ctttctctta caattcattc cttaagttct agtacgattt caggagaaac
      541 ctatgagctc ccagacccta accatcactg acaacagaac cggcaaacaa tacgaagtgc
      601 caatcgaaaa tggcacgatt aaggcgatgg atctacgaca gatcaaaacc gctgacgacg
      661 actttgggtt gatgacctat gaccccgcgt tcatgaatac ggcttcttgc aagagtcgca
      721 tcacgttcat cgacggtgac aaagggattc ttgaatatcg tggttatccc atcgaccagc
      781 ttgcggaaaa gagctcttat ctcgaggttg cttatctgct gctgcacggt gaactaccga
      841 acgaagcgga attgcgcgaa tggacctgga acatcacgca tcacaccttt atcaacgaga
      901 acatcaagaa agtaatcgat ggttttcact acaacgccca tcccatgggc atgtttgaag
      961 ccacgctcgg cgccctttcc actttctacc cagacgccaa agacatcttt aatgtggaat
     1021 gccgacgtaa acagatttac cggctgatcg ctaaagtgcc aaccatcgcc gcctttgctt
     1081 tccgccaccg gatcggaatg cagtattcct atcccgacaa tgatctgagc tacgaaggaa
     1141 acttcctcaa catgatgttc aagacgaccg agttgcagta caaaccggat ccggtgcttg
     1201 agcatgcgtt gactgttcta ttcattctgc acgctgacca cgaacaaaac tgcagcacca
     1261 atgccatgcg cagtattgga agtgcgcaga ccgatccgtt ttcagcgttg gggggcgctg
     1321 ccgcggcact ttatggtccg cttcacggcg gcgcgaacga gatggttttg cgaatgctga
     1381 aggagatcgg atcggttgat aaggtccccg attacgtgaa gcgggtgaag gcaggcgagt
     1441 tccgcttgat gggctttgga caccgcgtct acaagaatta cgatccgcgc gcccgcatca
     1501 tcaagcaagt tgcctatgaa gttttcgaag tcacgggcaa ggatccactc cttgacattg
     1561 ccctcgagct tgaacggatt gctctcgaag atgaatattt cgtgaagcgc cggctctatc
     1621 cgaacgtgga tttctactcg ggcatcatct atcaggcgat gcgtttcccg ctcgatatgt
     1681 ttccggtgct ctttgcgatt ccgcgaacct cagggtggct ggctcagtgg gtcgagttac
     1741 ttgaagatcc ggatcagaag attgcccggc cgcgtcaggt ctatctcggt cagcggacgc
     1801 gaaactatgt gccactcgcc aatcgaaaag ttcctgagac acagtccgcg ggagcttgag
     1861 ccggtcgcgg cttccactat atcgcgctcc gctcaatcgt ggacatgagt tgggcgcgat
     1921 acaattcaaa cgcgagtcga ttttgggctt cgctccaatc ggcatccgtg gactgaccat
     1981 cgggaaagaa atcgcaaaag acatctacca gcaccgcgcg caatttcacc tgacccaaca
     2041 gcgtctccag gtaacacgtg aaggagtgta ggggtgagcc gaccgctgcg ctctccggaa
     2101 cccgctgcaa gaggctctca ctaaggcttt caaactcatg ccagtcctta tacatcagga
     2161 actgcatcga ctcgtcgcgg aaggaactga tgctggttac gagtttggga aagttttgcg
     2221 cgatgtcatt ctcacatgcc tgcgcaaata ggatcatgtc tgtaagatcg cggcagagta
     2281 gcagcgactc acgaaggcgc gcctgatagt tatcaaatag tttcccgcca tcaagccctg
     2341 gctcgaacac ctgggcgagt gtaatgactg actgttgcag gcagttcgta agcaagccac
     2401 tcacgtaggc aatcttgccg cgcgcaacat tgtcaggttt ccaccccaat cccttaagtt
     2461 ccgaatcgaa agtgcgtttc agatcgtgac tcaacgcgaa ggcgatccca tccagagttt
     2521 ccgcgacaga tcgatccaaa ccctctgtcg cggttgccag actggcaatg agatcgacta
     2581 acgaggttgc ctcgcggcga aaacgctcaa ttaccacctt tgcgtccgcg ggattaggac
     2641 cttcgttcaa actcgccaat gcgggaacgt cctgcaacag gcttaagagc gccgaaaagg
     2701 cctcggacat catcttgcgc acttgctggt gctcaatctc ttccagcagt tgatcgattt
     2761 gctcgtgcgg gatctttggt tgagtacgga tgcttgacgg cgcactctca gcgacaacgg
     2821 gagggtcagg cagaagtgtt gattgcgaac tcataacttt gtagggaata gcgctttgga
     2881 ttgtgcggct tccggcgtga gagggaagcc ctgcggctgg acagcggggt ttgctgtctg
     2941 cgattgactt tacaacggag cctcaggact tgtcgagaaa ttctagtttt gctgaggaat
     3001 acccggaaac tccaactaag caaaagccag agcgggaaga ccatctgaac tcaaacgaga
     3061 agcataatca ggcgctcttg tttttcgtta cgctccaatt acttcaaa
//
LOCUS       DASUEX010000403         2321 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_640122, whole genome shotgun sequence.
ACCESSION   DASUEX010000403 DASUEX010000000
VERSION     DASUEX010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_640122"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..727
                     /locus_tag="VFV61_06770"
     CDS             <1..727
                     /locus_tag="VFV61_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900218.1"
                     /translation="EMRRLEPVSQLLERLTALEESLRTGKALAPAKPGPVTGGTAGPV
                     GGTSASTKGDSKAMSASVAGNAVKPALAFEQTVNHAEYSPPDSLPTVAPHTLANSAAV
                     AFAGDSEIERIKSALEKRRKMFLVTALEGARAAAIENGELYVEFAPEARHLRDSLAKS
                     DSVKVLREICQEITGKELGVRIMIKDQSVAVAEAPLSREDEERQEQNRLRENAEKHPV
                     VQQMLRTFRGELVDVRRVDKSES"
     gene            907..1116
                     /locus_tag="VFV61_06775"
     CDS             907..1116
                     /locus_tag="VFV61_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900219.1"
                     /translation="MRDPFTAGLLSFIIPGVGQLYNGRILAGILWLIITPGFWIGSGG
                     TLGWICHIISAYTAYSYAKEFPVRA"
     gene            complement(1165..2310)
                     /locus_tag="VFV61_06780"
     CDS             complement(1165..2310)
                     /locus_tag="VFV61_06780"
                     /inference="COORDINATES: protein motif:HMM:NF042449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase assembly
                     protein OpcA"
                     /protein_id="HEX4900220.1"
                     /translation="MSTSPKQIQVPKTLDVDAVERALAELWQETAGGEQVESDDAVLR
                     SRAANLMVVVTNEAALAGCQQIIRELAATHPCRALVMLADRDGADRDIEMFVSASCQD
                     QRRSSQAELSCEEITLVARGQFTAELPSAAIPLLVPDLPVFLWWQDLERLADESFQPL
                     SRAADRLVIDSVDLAAMDLQLGSIEQLFARQRNEEIAVSDINWARLTSWRALLANFYD
                     VQEYRPALADQERVRIDYAAPEGNPDGIATQALLMVGWLASRLDWRIERQEHTAAKTL
                     AFARRWDGGAVTFELNQVERPEMKPGRLARVELQSAHNHASFVVQRAENGLHLETQAT
                     VEGRLCPGRTLPVRNRSTAELLAREMEILARDTTYEAAVIAAGSLGS"
BASE COUNT          510 a          681 c          642 g          488 t
ORIGIN      
        1 ggaaatgcgg cggcttgaac cagtcagtca actgcttgag cgtcttaccg ccctggaaga
       61 gtctttgcga accgggaagg ccctcgctcc cgcgaagccg ggaccagtaa ccggaggcac
      121 tgcaggtcca gtaggaggga cttctgcttc gacaaaaggt gattctaagg cgatgagtgc
      181 ttccgttgcc ggtaacgcgg taaaaccagc gctggcattt gagcaaacgg tcaatcacgc
      241 tgagtattca cctccggatt ctctacctac tgttgccccg cacacgctcg ccaattcagc
      301 cgcagttgct ttcgcaggcg actccgagat tgagcgcatc aagtcggcat tggaaaaacg
      361 acgaaagatg tttctcgtga ccgcacttga aggcgcgcgt gcggctgcta tcgaaaatgg
      421 cgagttatat gtcgagtttg cgcccgaagc gcggcacctg cgcgactcgc tggcgaagag
      481 cgacagcgtg aaagttctgc gcgagatttg tcaggagatc accggcaaag aactgggggt
      541 gcgcatcatg atcaaggacc aaagcgttgc agtcgccgaa gcgccccttt cgcgcgaaga
      601 tgaagagcgc caggaacaga atcgcttgcg cgagaacgca gagaagcatc ccgttgtcca
      661 gcagatgctg cgcacgtttc ggggcgagct cgtagacgtc cgtcgcgtcg ataagtctga
      721 gagctaacgc gccacaaaaa gcgacaacct cgacatgtcg gggacgcact ccaaaggcca
      781 ggggcctggg gaatccaacc agcccccagt caatccgcca caattcctgc cacacctgaa
      841 gttaattttc cagcgaacta tttctagatg agggtcgtat aaagtcgctc ggaggtaata
      901 ctactcatgc gcgatccatt tactgcagga cttctcagct tcatcattcc cggcgtgggg
      961 caactttata acggacgcat cctggcaggc attctctggc taatcataac gccgggcttt
     1021 tggattggct ccggcggcac actcggttgg atctgtcaca tcatctctgc gtataccgct
     1081 tactcttacg cgaaggaatt tccggttcgc gcttaatgga tttggttgtt atctgaccgc
     1141 gggcgaagcg caagcaaact tgtcctaact tcctaaactc cccgctgcaa tgacggcggc
     1201 ctcgtaggtc gtatctcttg cgagaatctc catttcgcgc gccaatagct cagcggtgct
     1261 tcggttgcgc accggaagag tgcgacctgg gcacaaacga ccttccacag ttgcctgcgt
     1321 ctccaggtga agaccatttt ccgcacgttg caccacaaac gaagcgtggt tgtgtgcaga
     1381 ttgtagttcg accctggcaa gccggccggg cttcatttcg ggacgttcca cctgattcag
     1441 ttcaaacgtg actgcgccgc catcccatct gcgcgcgaat gcaagagtct tagcagcggt
     1501 gtgctcctgc cgttcaatgc gccaatccaa gcgactcgcc agccaaccga ccatcaacag
     1561 cgcctgggtc gcgatcccgt ctgggtttcc ctccggcgct gcatagtcga ttctgactcg
     1621 ctcctgatca gcgagcgcgg gccggtattc ctggacgtcg taaaaattgg cgagcaacgc
     1681 tcgccaggaa gtcagacgtg cccagttgat atcgctaacg gcaatctcct cgtttcgctg
     1741 acgcgcaaag agttgctcaa tactgcccag ttgcaagtcc atcgccgcca gatcaacgga
     1801 atcaatcacg agccggtctg cggcacggct cagcggctga aaactctcgt cggcgagtcg
     1861 ctcaagatcc tgccaccaga gaaataccgg gaggtcagga acaagtagcg gaatagcggc
     1921 actaggcagt tccgcggtga attgtccgcg agcgactaat gttatttctt cgcaactgag
     1981 ctcggcttgg cttgaacgtc gctggtcctg acaggaggcg gaaacaaaca tttcgatatc
     2041 acggtcagca ccgtcgcggt cggctaacat cacgagggcg cggcaaggat gagtagccgc
     2101 aagttcgcga attatctgtt ggcaccccgc gagcgccgct tcgtttgtca ccaccaccat
     2161 caggttggcg gcgcgcgagc gcagaaccgc atcgtcgctt tccacctgct cgcccccggc
     2221 agtttcctgc cacagttccg ccaacgcacg ctcgacagca tcgacgtcaa gtgtctttgg
     2281 aacctggatc tgttttggtg aagtcgacat tctctaatca g
//
LOCUS       DASUEX010000404         3490 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_513115, whole genome shotgun sequence.
ACCESSION   DASUEX010000404 DASUEX010000000
VERSION     DASUEX010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3490
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_513115"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="VFV61_06785"
     CDS             <1..606
                     /locus_tag="VFV61_06785"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEX4900221.1"
                     /translation="VQNNVKRMLAYSSIAHAGYALVGFVAAGAASTLEQRNSAIAAVI
                     FYLLTYALMNLGAFAIVQLVARAGDRRTEVEDYNGIGFQSPVLAFSLTLFLLSLLGMP
                     LTAGFIGKIMVFRAALDHGYYLLIVIGVLNTALSAYYYLRLIIVMFFRERTTSWSNPP
                     IPASIAVALVITLIGVLYLGLFPGRVINALQPKPIVTLSLR"
     gene            670..1155
                     /locus_tag="VFV61_06790"
     CDS             670..1155
                     /locus_tag="VFV61_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900222.1"
                     /translation="MTAEEYADQVKAELKDAWLPLIYRNRVLQMRTRSYYFGPLPQRP
                     ACEIQHTLLGVELKVGRRRLLCPDLATARYLSILARAGCENIAVPYDITKTSQLADEF
                     ESSWHRMLLLADRNAAAESDRFRTRLHGLLIAKLRAEVAAAGAGTARPEFKQDTKQRA
                     N"
     gene            1207..2142
                     /locus_tag="VFV61_06795"
     CDS             1207..2142
                     /locus_tag="VFV61_06795"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900223.1"
                     /translation="MSQEISELTAEEQQPTEAGGVFPFPYQHTFCRYEPISQRIEAAR
                     VLIVDNLLRDEADLSEVGRAEWGDAAATQLKRERLIAQMALENISNNIARLVKRPTTE
                     VAHLADVARAAARFKPDAIVLSGTLQDFDFYEPGIIESFGRFIRATKTPVLAICGGHQ
                     IVGLCFGAHVKTLDKLEQHEQRENRPHEYQYRFIRITDPQDPIFTGLKDPDTGALPRY
                     PRQPRILRVWQNHGLQLDQVPSGFKLLATSYLCRNQMMVKRSDGQLIYTVQFHLEKSF
                     EDWNKSRTRWEHQNESRDGRILFENFLNLALAHSS"
     gene            2102..2839
                     /locus_tag="VFV61_06800"
     CDS             2102..2839
                     /locus_tag="VFV61_06800"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900224.1"
                     /translation="MRTFLISRSRIHLNLRGAIPAAKRSRYVRSHLNYMAWDPLTEAS
                     LARATRILARRDRELAAIHERLGPPPLWARPPGFATLVKIILEQQVSLASAASIFARL
                     KKNTVPFTPARMIELGESHLKSLGLTRQKTAYCLHLAESLHDKRLRLSRLALMPDSEA
                     KAALMAIKGLGSWSADVYLLMVLRRPDIWPAGDLALATAAQKVKGLPQRPNPEQLIQL
                     AEGWRPYRSVAARMLWQFYLARRTEVS"
     gene            complement(2864..>3490)
                     /locus_tag="VFV61_06805"
     CDS             complement(2864..>3490)
                     /locus_tag="VFV61_06805"
                     /inference="COORDINATES: protein motif:HMM:NF016032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HEX4900225.1"
                     /translation="KQFPRTDIPDHFDSYSSFQRYVDLLVKTGCINDGKKIWWDLRPH
                     PIFPTLEFRICDIPTRVDDTVAIAALFQAIVAKLTKLIEKNLGFRLYRRMLIQENKWR
                     AVRWGIDGKMIDFGKQKEVPTRDLVLELLEFVDDVVDELDSRKEIEHIHTILERRTSA
                     EEQLRVFAETKDLNAVVDRLIELTMENVPVTTDFSAPAPVTTASTITK"
BASE COUNT          771 a          988 c          925 g          806 t
ORIGIN      
        1 gtgcagaaca acgttaagcg catgctggct tattcttcga ttgcccacgc cggctacgcg
       61 cttgtgggct ttgttgcagc aggagcggcg tcgacccttg aacagcggaa ctctgcaatt
      121 gccgctgtca ttttttacct cttgacgtat gccctgatga acctgggcgc gttcgccatc
      181 gtgcaattgg ttgcgcgcgc cggagaccgg cgtaccgaag tagaagacta caacggtatt
      241 ggcttccaat cgccggtgct ggcattctcg ctaacccttt ttttgttgag cctgctgggc
      301 atgccgctaa cggcaggctt catcggcaaa atcatggtgt tccgtgccgc actcgatcac
      361 ggctactacc tcctgattgt gatcggagtg ctgaacaccg cactctcggc gtattactat
      421 ttgagattga ttatcgtgat gtttttccgt gaacgaacaa ccagttggtc taatccgcca
      481 atcccggcaa gcattgctgt tgctctcgtg attactctga ttggcgtgct ctatctcggc
      541 ctcttcccgg ggcgcgtcat caatgcgctg cagccaaagc ccatcgtcac gctctcgttg
      601 cgctgatgcc ttttacctct ctgattctga tcgttcagca ccgacgcact ccgagaagtt
      661 tcttaaaaca tgactgctga agaatacgca gaccaggtga aagcagaact gaaagacgcc
      721 tggctacctc tgatctatcg caaccgagta ctgcaaatgc gaacacgctc gtattacttt
      781 ggaccgctgc cgcaaaggcc ggcctgcgaa atccagcaca ctcttctggg cgtggaactg
      841 aaagtgggcc ggcggcgcct gctttgtccc gatctcgcca ccgccaggta cctttcgata
      901 cttgctcgag cgggttgcga gaatatcgcc gtcccttacg acatcaccaa aacgtcacag
      961 cttgcagacg aattcgaatc ctcgtggcat cgtatgctgt tgctggccga tcgaaacgcg
     1021 gccgcggaaa gtgatcgctt ccggactcgc cttcatggcc tcctgattgc taagctgaga
     1081 gccgaggtgg cggcggcggg cgcgggaacg gcacgacccg agttcaaaca agacactaaa
     1141 caacgcgcca attaatggta tagtttgtcg gttgaattcc cgacatattg gacctttagc
     1201 taataaatga gccaagagat tagcgagctt acagccgaag agcagcaacc aaccgaggcg
     1261 ggcggagtct ttcctttccc gtatcaacat acattctgcc gctatgaacc gatctctcag
     1321 aggatcgagg cagcgcgggt cttgatcgtc gacaatctat tgcgcgacga agctgatctc
     1381 tctgaagtgg gtcgggcgga gtggggcgat gccgccgcca cgcaacttaa gcgcgagcgg
     1441 ctgatcgccc aaatggcgct ggaaaacatt tccaataaca ttgcccgcct ggtgaagcgt
     1501 cccaccaccg aggtggcgca cctggcggac gtggcccggg cggcagcgag gtttaagccc
     1561 gacgcgattg tattgagcgg cacccttcag gactttgact tttatgaacc ggggattatt
     1621 gagagctttg gaaggtttat tcgagctaca aaaactccgg tcctggccat ttgtggcggc
     1681 catcagattg taggcctgtg ttttggagcg cacgtaaaaa cgctcgacaa gctcgaacag
     1741 cacgagcaac gagagaaccg tcctcacgaa tatcagtacc gctttatccg aatcaccgac
     1801 ccgcaagatc cgatttttac gggtctaaag gatcccgata ctggtgcgtt gccacgttat
     1861 ccgcggcaac ctcgcattct tcgcgtgtgg caaaatcacg gactgcaact cgaccaggtc
     1921 ccctcaggct tcaaactcct cgcgacttct tatctttgcc gcaaccaaat gatggttaag
     1981 cgcagcgacg gccaacttat ctacacggtg cagtttcatc tggaaaagtc gtttgaggat
     2041 tggaacaaga gccggacgcg ctgggaacac cagaacgaat cgcgcgacgg tcgcatcctg
     2101 tttgagaact ttcttaatct cgcgctcgcg cattcatctt aatctccgcg gtgcaattcc
     2161 tgctgctaaa cgctcgcgct acgttcgctc tcacctaaac tacatggcgt gggatccgtt
     2221 gacagaagcc agcctcgccc gcgccacaag aattctcgcc aggcgtgatc gtgagctggc
     2281 ggcaattcac gagcgactcg ggccgccgcc gctctgggcg cggccgcccg ggttcgcgac
     2341 tctcgtcaaa attatcctcg aacaacaagt ctcacttgcc tcggctgcca gtatctttgc
     2401 gcgactcaaa aagaacacgg tgccgtttac tcccgcgcgc atgatcgagc tgggagagtc
     2461 acacttgaag tcgctcggct taacacgaca gaaaacagcc tactgtcttc atcttgctga
     2521 atcgcttcat gacaaacgct tgcggctgtc tcggctggca ctgatgcccg acagtgaggc
     2581 gaaggctgcg ttgatggcga taaaggggtt gggctcgtgg agcgcagatg tctatctgct
     2641 gatggttctg agaagaccgg atatttggcc ggctggggat cttgccctgg ctactgccgc
     2701 gcagaaggta aaaggcctgc cacaacggcc caaccccgaa cagttaatcc aacttgctga
     2761 gggctggcgt ccctatcgtt cggtggcggc gcgaatgttg tggcagtttt acctggcgcg
     2821 gcgtacggaa gtaagttagc aatttcgcac caacaaggat tacctactta gtaatcgtcg
     2881 atgcggtagt aaccggtgcc ggcgcactga aatcagtagt cacaggcacg ttctccatcg
     2941 tcagctcgat aagtcgatca actaccgcat tcaaatcttt ggtctcggcg aacactcgca
     3001 gctgttcctc cgctgacgtg cgtcgttcga gaatcgtatg aatatgctca atctcttttc
     3061 gcgagtcgag ttcgtccacg acgtcgtcga caaactccag taattcaaga accagatcgc
     3121 gcgtgggcac ttccttctgc ttcccaaagt cgatcatctt gccgtcaata ccccaacgaa
     3181 cagcgcgcca cttgttttcc tggatcagca tccgccggta gagacgaaaa ccaagattct
     3241 tctcgatcag ctttgtcagc ttggcgacaa ttgcctggaa cagcgcggct attgcgacgg
     3301 tgtcgtcaac gcgcgttggg atgtcacaga tgcgaaactc caacgtcggg aaaattggat
     3361 gcggacgcaa gtcccaccaa atctttttac cgtcgtttat gcaccctgtc ttcacaagca
     3421 agtctacgta acgctgaaaa ctgctatagg agtcgaagtg gtcgggaatg tctgtgcggg
     3481 gaaactgctt
//
LOCUS       DASUEX010000405         2474 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1112609, whole genome shotgun sequence.
ACCESSION   DASUEX010000405 DASUEX010000000
VERSION     DASUEX010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1112609"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            144..749
                     /locus_tag="VFV61_06810"
     CDS             144..749
                     /locus_tag="VFV61_06810"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HEX4900226.1"
                     /translation="MMTRDSVAGFILIGGAASRMGSDKSQLLIENETFVQRIARELAL
                     VTAQVSVVGRPEQFDPGLPVVYDLHPQWGALGGVHGALANCASEWALIVACDLPQVKG
                     ALFERLLSLRSGFEAVAPLQPDGRRQPLCALYRVVECLPRAAAMIESGERKPVALLQS
                     VRTRWVQFAELADLPGAEDFFDNINTPQDYLRISRETDRLD"
     gene            799..1755
                     /locus_tag="VFV61_06815"
     CDS             799..1755
                     /locus_tag="VFV61_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900227.1"
                     /translation="MARRRRLGKKFFKSLLPIVLLLVVALVVALAFIVYGVTRPPRGP
                     YLVTPEAFAEFSGPALKVSDQTWRNHDNTTARGWLLKGGAGAPAVILAHRYGVDRSWF
                     FNLGIKINEATGATILWPDLRGHGLNPPVSWTSFGSIEGEDLQAALVYLRTLKSVDGQ
                     VLVGDKVALYGVEMGAYASLRAASNGNGINVLVLDSLPRDPEEVLQAAVRNDLEIDNR
                     PTRFLTRAATKAYFLGRFDNTATCGLLAGLKEQKVLLLSGPDAGYLRDSTIDMAGCFT
                     NKANLEIKTDLPLTGFKLPSATGEQGEGYDRVVIDFLDRNFR"
     gene            complement(1757..>2474)
                     /locus_tag="VFV61_06820"
     CDS             complement(1757..>2474)
                     /locus_tag="VFV61_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900228.1"
                     /translation="DKLRVFSLAWLLLPLALFSFSGSKLPGYIIPVLPATGLLAGLRV
                     ARLTAQGSGRSMALGGTGALLLLGAIFGAVVSIRAGYLSVVGTAIVVAPLMIAGFINL
                     LFSQKRTLAVLSVVCALLLLPLLVLNSDLLRLTARESVRDLIAAADARGYGAAPLYGL
                     GEIDRTAEFYAAGRLAYDAEGETVIFENSVQVKDEAARRGTILVLVPREYDQLIGLRA
                     LGADVIGDNGKFVLVAVRSS"
BASE COUNT          556 a          687 c          690 g          541 t
ORIGIN      
        1 tgagaaaggg cggcctaagg ctcacggagc atgattgacg aggttttctg taggaactgg
       61 ccggggcccc ccccgggtag atttcggagc tgagggtgat cgggcaaggt cactaacgct
      121 accaaggacg ccaaaacatt cgaatgatga ccagggattc agtcgcaggt tttattttga
      181 ttggcggagc tgcaagccgc atgggcagcg acaagtcgca gctcctcatc gagaacgaaa
      241 cctttgtgca gcggatcgct cgtgaactgg cgctggtgac tgcgcaggtt tcagttgttg
      301 gaaggccaga gcagttcgat ccgggcctgc cggtcgtgta cgacctccat ccacagtggg
      361 gagcattagg tggcgttcat ggtgctctcg ccaattgtgc ttccgagtgg gcattgatcg
      421 tggcttgtga tcttccgcaa gtgaagggcg cactgtttga acgattactt agtttgcgta
      481 gcggctttga agcagtcgcc ccgcttcagc ccgatggccg ccgccagcca ctctgtgccc
      541 tgtatcgtgt ggttgaatgt cttccccggg cagcagcaat gattgaaagt ggggagcgaa
      601 aaccagtggc tctgctacaa tccgtgagga cgcgctgggt gcaatttgcg gaactcgctg
      661 acttgccggg cgccgaggat ttctttgata acatcaacac gccccaggac taccttcgca
      721 tcagccggga aactgatcgg cttgactgaa acgctgaact tattgcctca aacgttctga
      781 ttcctcccat tcgcgaatat ggcccgacga agacggttag gtaagaagtt cttcaagtcg
      841 ctgctaccga tagtgctctt gctggtagtg gcgctggtcg tggctctggc gttcatagtt
      901 tacggcgtca cgcggccgcc acgaggtcct tatctcgtca ctcccgaagc gttcgcagaa
      961 ttcagcggtc cggcccttaa ggtttccgat caaacctggc gcaatcacga caacaccacg
     1021 gcccgaggtt ggctactaaa aggtggcgcc ggcgccccgg cggtgatact ggcgcatcgc
     1081 tacggcgtcg atcgttcgtg gttcttcaat ctcggaataa agatcaatga agccacgggc
     1141 gccactattc tctggcccga cctgcgaggt cacgggctaa atccgccggt ttcctggacc
     1201 tcattcggtt caattgaagg cgaagacctt caggcagcgc tggtttacct tcgaaccctg
     1261 aagagtgtgg atggacaggt actcgtgggt gacaaggtgg ctctctacgg agttgagatg
     1321 ggcgcctacg cttcgctgcg ggctgcgagc aacggcaacg gcattaatgt tctggttttg
     1381 gattcgctgc cgcgggatcc agaggaggta ttgcaggctg cggtccgcaa cgacttagaa
     1441 atagacaatc gcccgacacg atttcttacg cgtgccgcaa cgaaggccta cttccttggt
     1501 cgatttgata atactgccac gtgcggactc ttggcgggct tgaaagaaca aaaagttctg
     1561 ctgctttccg gccccgatgc aggttatttg cgcgactcta cgatcgatat ggctggctgc
     1621 ttcaccaaca aagctaatct cgaaattaag actgacttgc cgttgactgg ctttaagctg
     1681 ccatccgcaa ctggtgagca aggtgagggc tatgatcggg tggtgattga tttcttagac
     1741 cgcaacttcc gctaattcag cttgacctta ctgcaaccag aacaaacttt ccgttgtccc
     1801 caatcacgtc agcccccagc gctcttagac caattagttg atcgtattcc ctgggaacca
     1861 gaaccaggat cgttccgcga cgagccgcct catcctttac ctgcactgag ttttcaaata
     1921 ttaccgtttc gccctccgcg tcatatgcca ggcgtcctgc tgcataaaac tccgccgtgc
     1981 gatcaatctc gccgagacca tagagtggag ctgcgccgta gccgcgcgca tcagctgcgg
     2041 caatcaaatc gcgcacagat tcgcgagctg tcagtcgcaa cagatcgctg ttcagaacca
     2101 ggagaggtaa caagagcagt gcgcaaacta ccgacaagac tgcgagggtt cgtttttgcg
     2161 aaaacagtaa gttaataaaa cccgcgatca tcagaggagc aaccacgatc gctgtgccaa
     2221 ccactgaaag atagccggct cgaatagaca ccaccgcgcc aaagattgca cccagcagga
     2281 gtagcgcgcc agtccctccc aacgccatag accgtcccga tccctgagcg gtaagtctcg
     2341 caacccgcag ccccgccaac aatccagtgg ccggtaatac aggaataatg tagcccggga
     2401 gttttgagcc tgagaacgaa aagagcgcga gcggcagcag caaccaagcg aggctgaaga
     2461 ctctcaactt gtcc
//
LOCUS       DASUEX010000406         2036 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1655197, whole genome shotgun sequence.
ACCESSION   DASUEX010000406 DASUEX010000000
VERSION     DASUEX010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1655197"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /locus_tag="VFV61_06825"
     CDS             <1..490
                     /locus_tag="VFV61_06825"
                     /inference="COORDINATES: protein motif:HMM:NF014456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900229.1"
                     /translation="GVVGYAPNNTYVVRDADEVPSLPYLRSPIVVSQTTIKLKTFLDV
                     AEAVREKSDTEPEVINTICSATRDRQDAARALAGQVDAFYVIGGRHSSNSVKLLAVCQ
                     EQCERSFLIETPAEINPQDLQGVKRVGVTAGASTPNWLIDQVVERLREIDGEQTAVLS
                     ET"
     gene            554..1165
                     /locus_tag="VFV61_06830"
     CDS             554..1165
                     /locus_tag="VFV61_06830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900230.1"
                     /translation="MIDDFGARVASIWSELRPTTRGLVERALKLPSTSPLPGRNLPYD
                     ARADLELSRLLSALDDRAAETSASPATSRDEQLRRLADTCAAVLQEKTQSAEVFTQLI
                     KRAGAQRDYARIDSLADELTRFAPSEICELARSEDLVARALANEALAQCPTGVLVALL
                     SDLVDSEIARQALRRQALEFGSEEARQIVTMLDQVDAADEEFQ"
     gene            complement(1184..1405)
                     /locus_tag="VFV61_06835"
     CDS             complement(1184..1405)
                     /locus_tag="VFV61_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900231.1"
                     /translation="MEVTKALAALQPLYGKNNIEIVTVDGRRVRGLVLSMKTTQALTT
                     PSVKIECANGEVVKIPFPAIAEIKDHNAA"
     gene            1708..>2036
                     /locus_tag="VFV61_06840"
     CDS             1708..>2036
                     /locus_tag="VFV61_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900232.1"
                     /translation="MPRQLRFCRNCGFRLGEGLAEYTETVRFNGQQAATMLDRTTASA
                     QPFVTSYGLTGGGIAPAAPSALKKRKKMSGMTWIFLGLLVFFITAAAFTAIVTPIRRG
                     TNISIGVR"
BASE COUNT          443 a          556 c          559 g          478 t
ORIGIN      
        1 tggagtagtg ggctacgctc cgaacaatac ttatgtcgtg agagatgccg atgaggtccc
       61 ctctctgcct tacctgcgct cgccgattgt agtgtcgcag actacgatca agttgaaaac
      121 gttcctcgac gtggcagaag ccgtgcgaga gaagtctgac actgagccgg aagtcatcaa
      181 cacgatctgc tcagcaacac gcgaccgtca ggatgcagct cgcgccctcg ccggccaagt
      241 cgatgccttt tatgtcattg gcggtcgtca ctcgtctaat agtgttaagt tgctggcggt
      301 ttgccaggaa caatgtgagc gcagcttttt gatcgaaaca ccggcggaga ttaacccgca
      361 ggatcttcag ggagtaaaac gcgtcggcgt aacggcgggc gcctcgacac ccaactggct
      421 gattgatcag gtggtagaac ggcttcgtga aattgatggc gagcaaacag ctgtacttag
      481 cgaaacctga ttcgagcccg actccaccta ctactgccta ctccaaaacg ggggacctcg
      541 caatttgtca ctgatgattg atgattttgg cgcacgggta gcgagtatct ggtcggaact
      601 ccgtcccact actcgtggac tggtcgaacg cgcgttgaaa cttcccagca cttcgccttt
      661 gccgggtcgg aacctcccct acgacgcgcg cgcggatctc gaattgagcc gtctcttgtc
      721 ggctcttgat gatcgcgcgg cagaaaccag tgcatcacct gcaaccagca gagacgagca
      781 gttgcggcgc ctggctgaca cctgtgcagc cgtgctgcag gaaaagaccc agtcggcaga
      841 agtctttaca cagttgatca aacgagctgg cgcccagcgg gactatgcac gtattgattc
      901 gcttgcggat gagttgacga gattcgcgcc gagtgagatt tgcgagctgg cgcgttccga
      961 ggatctggtg gcgcgcgctt tagccaacga ggcattggcg caatgtccga ccggggtttt
     1021 ggttgcgttg ttgagcgatc tggttgattc agaaattgcc cgtcaggcct tgcgtcgtca
     1081 agcgctggaa ttcgggtcag aggaagctcg ccagatcgta actatgctgg atcaggtgga
     1141 cgcggcagat gaggagtttc agtgatcagc tgaaaccgcc gacctacgct gcattgtgat
     1201 cctttatctc tgcaatcgca gggaagggta tcttgacaac ctcgccgtta gcgcattcga
     1261 tcttgacgct tggcgtcgtt agcgcttgag tcgtcttcat gctcagcacc aaaccgcgca
     1321 ctcgccgccc gtccacagtc actatctcaa tgttgttttt accgtaaagc ggttgcaagg
     1381 cagcaagagc tttggttact tccacaagcg atccccctta gacaattgtc tcgagtgttt
     1441 tcaagatatt gcaccttcct tgctgagaag tccattagtg gcatcgaacc agtcgtatgc
     1501 cgatcggcga ccaatccagt ccctctctgg atgcagccaa aatgcaaccg cagggcgtaa
     1561 tttgacgtat agtcaagtgc cgtaagcgaa agatggctga attacccccc tcaaattggt
     1621 aggttcacca tgtcacagaa tcccgcttta tccgcagact ctggcaacga ttcaggctca
     1681 atcgcctgtc cgaattgcca ttcgcccatg ccccgccagt tacggttctg tcgcaactgc
     1741 ggctttcgtt tgggagaggg tcttgccgaa tacaccgaaa ccgtccgttt caacgggcag
     1801 caggctgcga ccatgttaga ccggacaact gcgtctgctc agccgtttgt cacgagttat
     1861 ggactaacgg gaggaggaat tgcgccggca gctccatcag ccctgaagaa acgaaagaag
     1921 atgagcggta tgacctggat ctttcttggt ctgctcgtgt tctttattac tgcagccgcc
     1981 ttcactgcca tcgttacgcc cattagacgt gggacaaata tctctattgg tgtccg
//
LOCUS       DASUEX010000407         3046 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1113567, whole genome shotgun sequence.
ACCESSION   DASUEX010000407 DASUEX010000000
VERSION     DASUEX010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3046
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1113567"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="VFV61_06845"
     CDS             <1..321
                     /locus_tag="VFV61_06845"
                     /inference="COORDINATES: protein motif:HMM:NF012928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HEX4900233.1"
                     /translation="NRLLVPYMLDAIRALEEGVGSIVDIDLAMKLGCGYPMGPFTLGD
                     FVGLDTTYYIAEIMFNEFREKRFAPPPLLKRMVLAGLYGRKSGRGFYDYTTDPKNPTP
                     MNLV"
     gene            382..1125
                     /locus_tag="VFV61_06850"
     CDS             382..1125
                     /locus_tag="VFV61_06850"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX4900234.1"
                     /translation="MAMVRLNRPEKLNALSGALIRELTQLLNELRQDQLLRAVILTGT
                     GERAFCVGTDINEIAGLNKDEARAVSRRGQELCNLISEFPVPVIAAVNGLAVGGGCEL
                     ALACHLRVASTNASFSLPEVKLGIIPGYGGSQRLAREIGRGRAIEIMLTGRSVAAEEA
                     QRIGLVNQIAQAELLLEDSKSLAQQIAQLAPLAIRACLTAVNRGLELPLAEGMALETK
                     IFADLFSTADMREGTNAFLEKRTPVFTGT"
     gene            1249..1737
                     /gene="ribH"
                     /locus_tag="VFV61_06855"
     CDS             1249..1737
                     /gene="ribH"
                     /locus_tag="VFV61_06855"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: protein motif:HMM:NF013081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEX4900235.1"
                     /translation="MQPLTHEGKLNAAGYRFAIVAGRWNDFITSRLVEGAVDALERLG
                     AEEKRVEVYRVPGSFEIPLLALRLAGSGKFDAVICVGTIIRGQTPHFDYIASEVARGI
                     SQAGMQTGVPVVFGIVTADTLDQAIDRAGVKLGNKGFEAATTAVELVNLYKEVFEKKD
                     VG"
     gene            1810..2277
                     /gene="nusB"
                     /locus_tag="VFV61_06860"
     CDS             1810..2277
                     /gene="nusB"
                     /locus_tag="VFV61_06860"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEX4900236.1"
                     /translation="MGSRRKARECALQMLFAADVAETPADEVVRTYWAELGEEEIEPA
                     ARDFASRLASGTLSHLELLDERIRSRAEHWRISRMAVVDRNILRLAVYEFLYEPTPRT
                     VAINEALEIARRFSTYEATQFINGILDAIKGDLDEQQPQEHLAGEAQEEEEQS"
     gene            2400..>3046
                     /locus_tag="VFV61_06865"
     CDS             2400..>3046
                     /locus_tag="VFV61_06865"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HEX4900237.1"
                     /translation="MDQSGPDFQSEIAELLERFFQDLEKLREEFADVSARRNLIGSIF
                     RRVHSLKGCANTAALEETGTIAHHLEDLLADIRDHKVELTESLLDLIEDAGNLITATL
                     GPAPPAGTSIEGFLSYLQAVERTRLPAHVSACPPTLPSDIWQSLNETQQRHLVRALDD
                     GLELYIITTSFDIADFDQQFYRLKETLDDLGEVISTYPSVGFAQTHGINFRILFV"
BASE COUNT          706 a          771 c          851 g          718 t
ORIGIN      
        1 aaccgtctct tggtgcctta catgctggac gctatccgcg cgctggaaga gggagtcggc
       61 tcgatcgtgg atattgatct ggcgatgaag ctgggatgcg gctatcccat gggaccattc
      121 acgctcggtg atttcgttgg gctggatacg acctactaca tcgccgagat catgttcaat
      181 gaatttcgag agaaacgctt tgcgccccca cctctattga aacgaatggt cctggctggg
      241 ctatacggtc gcaaatcagg tcgtgggttt tacgactaca cgaccgatcc aaaaaacccg
      301 actccgatga atctggtttg agttctgtcg ttaacgttac ttaccttgtc atcactcatc
      361 cagtccgaac aaattggccc aatggcgatg gtacgtctga accgtccgga gaagctgaat
      421 gcgctctcag gggcgctgat ccgcgaactc acccaattgt tgaacgaact tcgtcaggac
      481 caattgcttc gcgcggtgat tctgaccggg actggtgagc gggcgttttg cgtaggcact
      541 gacatcaacg agattgcagg gctgaacaag gatgaagcgc gtgccgtctc ccggcgcggc
      601 caggagctgt gcaacctcat aagcgaattt ccggtaccgg tgatcgcggc agtcaatggg
      661 ttggcggtgg gcggtgggtg cgagttggcg ctggcttgtc acctgcgagt ggcatccacc
      721 aacgccagtt ttagtctgcc cgaggtcaaa ctaggcatca ttcccggcta cggcggctca
      781 caaaggctgg caagagaaat cgggcgtggc cgggcaatcg aaataatgct gacgggcagg
      841 agcgtggctg cagaagaagc tcaacgtatc gggctggtga atcagatcgc ccaggctgag
      901 ctgttgctgg aggattcaaa atcattagcg caacagatcg ctcaactcgc cccgctcgct
      961 attcgcgctt gcctcacagc cgtgaatcgt gggcttgagt tgccgttagc agagggcatg
     1021 gctttggaga cgaagatttt tgctgacctt ttttccaccg ccgatatgcg cgaaggcacc
     1081 aacgcgtttc tcgaaaagag gacgccggtg tttaccggga cgtgatgagt ttcatcgaaa
     1141 gcgccgcaca agttttgggg ttaagaccaa cttgccgctc gcaaaggcct tcacctatac
     1201 tgcaaaagta ttcatgaacg ccgattggaa acctggtagc gaagtctttt gcagccatta
     1261 actcacgaag gaaagctgaa tgccgccggt taccggtttg ctattgttgc cgggcgctgg
     1321 aacgatttca ttacgtcccg tctggtggaa ggggcggtgg atgctctcga acgtttgggc
     1381 gcagaagaaa aaagggtgga ggtttatcgc gttccaggtt cttttgagat cccgctgttg
     1441 gctctgagac ttgccggttc aggaaaattc gatgcggtga tatgtgttgg gacgatcatt
     1501 cgcggacaaa ctccacattt tgactacatc gccagtgaag tggcgcgggg aatttctcaa
     1561 gcaggaatgc aaacaggagt gccagttgtt tttggaattg tgacggccga tacgctcgat
     1621 caagcaatcg atcgcgccgg agtcaaactg ggcaacaaag gttttgaagc tgccactact
     1681 gcggtggaat tggttaatct ttacaaggaa gtctttgaaa aaaaggatgt aggatgaaga
     1741 gtgaaggagg tggatggatg cggcagccgt ttcgtccttc atccttccag attcatccct
     1801 tgaagtatta tgggttcgag acgtaaggcg cgtgaatgcg ccttgcaaat gctctttgct
     1861 gctgatgtag ctgaaacgcc cgcggacgaa gttgtgcgga cgtattgggc tgagctgggc
     1921 gaggaagaaa tcgagccggc cgcgcgggac tttgcgagcc gcctggctag tggcacgctg
     1981 tcacaccttg aactgctgga tgaacgtatc cgttcgcgtg ctgagcattg gcgtatttcg
     2041 cggatggcag tagttgatcg aaacattctc cgcctggccg tctacgagtt tctctacgaa
     2101 ccaactcctc gtacagttgc gataaacgaa gcacttgaga tcgcccgccg tttctcaacc
     2161 tacgaagcaa cgcagtttat aaacggcatt ctagatgcca tcaaaggcga tctggacgag
     2221 cagcaaccac aagagcacct cgccggtgaa gcgcaggaag aggaagagca atcgtaaact
     2281 cttacacaca gatcattgcc tgcctcagac tttccgtggc gtcccccgtt cagtgttaac
     2341 acattcgact gcggcgccaa tatttcgctc attaacaacc tactgattac tatcttaacg
     2401 tggaccaatc cgggccagac ttccaaagtg agatcgctga attactcgag cgtttctttc
     2461 aagacttgga aaagttgaga gaagagttcg cggatgtttc ggcgcgtcgt aacctgatcg
     2521 gcagcatctt tcgccgggtt cacagcctaa aggggtgtgc gaataccgcg gccttggagg
     2581 aaaccgggac gatcgcccac catcttgaag atctgctcgc agatattcgc gatcacaaag
     2641 ttgagttgac tgaatcgctg ctcgacttaa tcgaagatgc cggtaacctg attacggcga
     2701 ctctcggtcc tgctcctcca gcgggaacgt ccattgaagg atttctgagc tatctccagg
     2761 cagtggaacg gacaagactg cctgctcacg tttctgcttg ccctccaacc ctcccatcgg
     2821 acatctggca gtctcttaat gagacccagc aaaggcatct ggtcagagcg ctcgacgatg
     2881 gccttgagct ttacatcatt acaaccagtt tcgacattgc cgatttcgat caacagtttt
     2941 accggctgaa agagacgctc gacgacttgg gagaggtaat ctcaacctat ccgagtgtcg
     3001 gctttgcgca aacacatggc ataaactttc gcatcctttt cgtcat
//
LOCUS       DASUEX010000408         3324 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_878960, whole genome shotgun sequence.
ACCESSION   DASUEX010000408 DASUEX010000000
VERSION     DASUEX010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3324
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_878960"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            148..483
                     /locus_tag="VFV61_06870"
     CDS             148..483
                     /locus_tag="VFV61_06870"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX4900238.1"
                     /translation="MDPVHDASNLYEVERRALHAERPGFRISELQISPTQKVPWHYHN
                     NVQDTFYVITGRIRIVLADPAEETALGAGEVFSVAAGRPHFVKNDGDASATFLILQGG
                     GEYDYVPLT"
     gene            complement(563..1168)
                     /locus_tag="VFV61_06875"
     CDS             complement(563..1168)
                     /locus_tag="VFV61_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900239.1"
                     /translation="MRVQKILIKFCISGLLAGAVAAASASAVLAQDEIYEGSTVTGTA
                     VAFSGRLGSRSSNFRLVIRNYTSRGEVERLNEVLRSGQDKLLNAISGMSAGQIQIGNG
                     VGVPANVIFAEPSGNGETKITVVYQRYIGFSELRYGTRSADYRFGYAELFIRRNGAGQ
                     GTLIPAAKIRLRDGNTWEVEDFGIYPARLLGLRVRGGRVPR"
     gene            complement(1449..2435)
                     /locus_tag="VFV61_06880"
     CDS             complement(1449..2435)
                     /locus_tag="VFV61_06880"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEX4900240.1"
                     /translation="MSNGILVFIEHRKGVLNKTSLEAIAAGQSLAAQLQQPVSAILLG
                     SDVSALAQEIVAYDLAKVLCAHNAGLGEYTPDGYSAAMESVIRKLDPQLVVMPHTYLV
                     RDYAPKLAARFGKSLISDCIRAQVAGTSITFTRRIFLGKLDSDVVSDGEPPMFATFQS
                     GAYRPDQARKGGSAAVETIEVEVGEIRMTPEEPFQEVKQAVDLSKAEIIVAIGRGIKG
                     KENIALAEKLAEVLGGDIAASRPICDAEWLPIDRQIGSSGQTVAPKLYIALGISGAIQ
                     HLVGMKNSGTIVAINKDPEAPIFDIADYGIVGDLFEAVPVLTEELKKIKGLV"
     gene            complement(2459..3232)
                     /locus_tag="VFV61_06885"
     CDS             complement(2459..3232)
                     /locus_tag="VFV61_06885"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEX4900241.1"
                     /translation="MKIIVLMKQVASKDAILRINKDATWIEEADLSFEVSESDGYALE
                     EALRVREKLTGEVVVCSMGPARVKSVIKDALARGADRAIHVAGDNLGNLSPFASASAL
                     AEAIRDEKPDLILTGLQSDDFGYGQTGVILAELLGLPHATIVIELDASGAKLRVKREL
                     ESGWYQWYSMPMPALLTIQSGISQIRYATLKGIMAAKKKEIREVSPSPEVVGRPNHQR
                     IEKIYLPQKTKQTQLLGGGDAKAGAVALAEKLHTEARVV"
BASE COUNT          717 a          944 c          857 g          806 t
ORIGIN      
        1 atatcgcgac aattcctgga aacccgatga ggtggttgcc gaattgagga agatgcaacc
       61 gtaaaggccg gaggattgct aattgccgat taacctcttg cgggccagat caattggcaa
      121 tcggaactcg aaaatcggaa atccagtatg gatccagttc acgacgccag taacctctac
      181 gaagtcgaac ggcgcgcttt acatgcggag cgccccggct tccgtatcag tgaactgcaa
      241 atctcgccga cgcagaaagt gccctggcac tatcacaaca acgtgcaaga cacgttctat
      301 gtgatcactg gaaggattcg gattgtcctg gccgatcccg ccgaagagac tgcgcttggt
      361 gccggagaag tattttcagt agccgcaggg cgcccgcact tcgtcaaaaa cgatggtgat
      421 gcctccgcca cgtttctgat cctgcaaggt ggcggcgaat acgactacgt cccgctcacc
      481 tgatcacttg cgagaatccc ctggcccgcc ggcagactgg aatccctacc gttagcttcg
      541 ggttcacctg gtgccgactt aactagcgcg gcactctgcc accacggacg cgcaagccca
      601 gcaaacgcgc gggatagatt ccaaagtctt ccacttccca ggtgttgcca tctcgcagcc
      661 tgatcttcgc ggccggaatc agtgtcccct gacctgctcc attgcgccgg ataaaaagct
      721 ccgcgtaacc aaagcggtag tctgccgaac gcgtaccata acgcagctca gagaagccga
      781 tgtatcgttg atagacaacg gtgattttgg tttcaccatt ccccgacggc tcggcaaaaa
      841 tcacgttcgc gggaacaccc actccgttgc caatctgaat ctgtccggca gacattccgg
      901 aaatggcatt cagcaacttg tcctgacccg atcgcagtac ctcattcagc ctttccactt
      961 ctccgcgact ggtatagttc ctgataacca gtcgaaagtt gctggagcga ctgcctaggc
     1021 gtccgctaaa cgccacggca gtgcccgtga ctgtcgaccc ttcatagatt tcgtcttgtg
     1081 ccaacaccgc gcttgctgac gcagcggcga ccgctcccgc gagcaagccg gaaatgcaga
     1141 acttaatcag gattttctgg actctcataa tcttcacctt tagcgatctg caacgatcgc
     1201 cgaaacactt cgccgtttga gaccgaatgt tcgtgttaac tgttctgatg cgcagcacac
     1261 aggtctcggt ggcttccgca ttcacaatcc ggttgagatt tgatcctatt accattgaga
     1321 tcttaatgcg actcagtgcc aacgcgaaga aaagagccca acacgtcgag tgttgggctc
     1381 gttctacttt ggggcgaacg tgcgcagggc tggtcgcgag gttcaggatt caaataatct
     1441 gcggttcgct agactagtcc cttaatcttc ttgagttctt cggtgaggac aggaacggcc
     1501 tcgaagagat ctccgacgat gccatagtcg gcaatgtcga agattggagc ttccggatct
     1561 ttgttgatgg cgacaatcgt tcccgagttt ttcataccaa ccaaatgctg gatggcgcct
     1621 gagatgccga gggcgatgta gagcttgggc gccacggttt ggcctgacga tccaatttgc
     1681 cggtcgattg gtaaccactc tgcgtcgcaa attggtcgcg atgctgcgat gtcaccgcca
     1741 aggacttccg ccagtttctc ggccagggca atgttctcct tgcctttgat tccgcgacca
     1801 atcgccacaa taatctccgc cttcgacaaa tcgacggctt gcttcacttc ctggaatggc
     1861 tcttctggcg tcatccggat ttcaccgact tccacttcaa ttgtctcgac ggcggcactg
     1921 cctcccttgc gagcctgatc cggccggtag gctcccgatt ggaaggtggc aaacattgga
     1981 ggctcgccat ctgagacaac atccgagtcg agctttccca ggaagatgcg gcgcgtaaaa
     2041 gttatgctgg tgccggctac ttgcgcacga atgcagtcac tgatcaatga ctttccaaaa
     2101 cgcgcggcca gcttcggtgc gtagtcgcgc accaggtagg tatgcggcat tacaactagc
     2161 tgcggatcaa gttttcgaat cacgctttcc atggccgcgc tgtagccgtc cggcgtgtat
     2221 tcaccaagtc ccgcattgtg ggcgcacaaa acttttgcca ggtcgtacgc gacgatctcc
     2281 tgagccaagg cgcttacatc tgatcccaga agaatcgcgc tcaccggttg ttggagctgt
     2341 gcggcgaggc tctgacctgc ggcgattgcc tccagcgaag tcttgttaag gacgcccttg
     2401 cgatgttcta tgaaaactaa gattccgtta ctcatgaata atctctctat ggacgatctc
     2461 aaaccactcg cgcttctgtg tgaagtttct ctgcgagggc caccgcgcca gctttggcat
     2521 cgccaccgcc taaaagctgc gtctgcttgg ttttttgcgg caggtagatc ttctcgattc
     2581 tttgatgatt gggccggccg actacttccg gtgaaggcga tacttctctt atctctttct
     2641 tctttgccgc catgatgccc ttcagagttg cgtagcgaat ctggctgatg ccggactgaa
     2701 ttgtcagcag ggcgggcatg ggcattgagt accattgata ccagccgctc tccaattcgc
     2761 gttttacgcg caacttagcg ccggaggcat cgagctcaat cacgatcgtc gcatgcggta
     2821 aacccaggag ctccgcaagg attactccgg tttgtccgta gccaaagtca tctgattgca
     2881 gtccagtcag aatcaggtct ggcttttcat ctcggatggc ctcggctagc gcgctggcag
     2941 aggcgaacgg agaaaggttt cccagattgt cgccggcaac atgaatcgct cgatcagcac
     3001 cacgcgcgag agcgtctttg ataacactct taacccgagc cgggcccatg gaacagacaa
     3061 ctacttcgcc ggtgagcttt tctcgcacac gcagcgcctc ttctaacgcg tagccatcgg
     3121 attcactaac ttcaaacgat agatctgctt cctcaatcca ggttgcgtct ttgttaatcc
     3181 gcaagatggc gtccttactg gcgacctgct tcattaaaac tatgattttc atcgtggtta
     3241 gatttgtcgt cgcttgagtt atttggtctt tggacttggg tctttggtct ttgtgataag
     3301 gagatcgggt gactcacact tcaa
//
LOCUS       DASUEX010000409         5255 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1113867, whole genome shotgun sequence.
ACCESSION   DASUEX010000409 DASUEX010000000
VERSION     DASUEX010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5255
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1113867"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..604
                     /locus_tag="VFV61_06890"
     CDS             <1..604
                     /locus_tag="VFV61_06890"
                     /inference="COORDINATES: protein motif:HMM:NF003995.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEX4900242.1"
                     /translation="ALAEQFNLNSAQIRKDLGYFGEFGVRGVGYYVADLRAHLTKILG
                     LDTAHRVGIVGAGRLGTALANYQGIEKSNFSVVALFDNDSGKIGTRIGPGNVLVHDVK
                     RIARVVKDKSVDVAVIAVPARAAQRVLNQVTGAGVRAVLNFAPASLSARLGVKVKTVD
                     LTTSLESLSYFLSRPQASVTTKARRGAEPEELPAHSEREL"
     gene            623..1441
                     /locus_tag="VFV61_06895"
     CDS             623..1441
                     /locus_tag="VFV61_06895"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX4900243.1"
                     /translation="MDEQTARREIVRIGQLMYERSYVVSSDGNVSVRLDDGRILATPT
                     MTCKGRMTEDSLALTDATGKALSNRKASSELAMHILIYNERPDVKAVCHAHPPHGTAF
                     AVAGLAIDQPILSEVILTLGCVPLAAYGTPSTEELTEAMRPLVKHHNALLMANHGAVA
                     YGSDLWQAFDRLETLEHTARIAILSRILGGSKNLPVDAIEKLINVREAAGYLGESARC
                     QACGYLHETQLTCPTGDRPPVSGYHEPATNGSGKIALTREELVELLAQAVSRES"
     gene            1522..1806
                     /gene="eutM"
                     /locus_tag="VFV61_06900"
     CDS             1522..1806
                     /gene="eutM"
                     /locus_tag="VFV61_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HEX4900244.1"
                     /translation="MPQEALGMVETKGLVAMIEAADAMVKAANVTLIGYEKIGAGFVT
                     AIVRGDVAAVKAATDAGAAAARRVGELVSVHVIPRPHGSVDDALPIGNRG"
     gene            1808..2077
                     /locus_tag="VFV61_06905"
     CDS             1808..2077
                     /locus_tag="VFV61_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002915814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HEX4900245.1"
                     /translation="MIIARILGTVVATQKDERLLGKKLLIVRPVNVDGTDTTGYVVAV
                     DTVGAGFHEKVLVVAGSSARLAQGMKDVPVDAAVVGVIDTVDITS"
     gene            2175..2474
                     /locus_tag="VFV61_06910"
     CDS             2175..2474
                     /locus_tag="VFV61_06910"
                     /inference="COORDINATES: protein motif:HMM:NF015289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HEX4900246.1"
                     /translation="MQLARVIGTVVATAKNESLEGRKLLVVQTLNRDLQPSGKPLVAV
                     DSVGAGLGELVFWCRGKEASFPFKREDTPTDCTIVGIVDSEFHVTQSASYQKRIS"
     gene            2479..2751
                     /locus_tag="VFV61_06915"
     CDS             2479..2751
                     /locus_tag="VFV61_06915"
                     /inference="COORDINATES: protein motif:HMM:NF015289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HEX4900247.1"
                     /translation="MILARVLGNIVATQKNRRYENASVMLCQQITPEGDETSYTVLAL
                     NSVDAGVGDTVLIVQEGWSASTAATGKPGAAIDSAIVGVVDHIDLL"
     gene            2758..3210
                     /locus_tag="VFV61_06920"
     CDS             2758..3210
                     /locus_tag="VFV61_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900248.1"
                     /translation="MSTDQDQAREIAERIARRVSGSSLSSSSAGRQQGSEVSSQLAAV
                     RAGINDLQRKLAQLEAAVSSAPSAPTVPQMSPTQGAMSPHRSGHVTHSPWLAGINSSY
                     SHPSEEKFGVEEATVSALVDFFEKEKLCSVEPGGKPCDHCSMCSSRGF"
     gene            complement(3267..3890)
                     /locus_tag="VFV61_06925"
     CDS             complement(3267..3890)
                     /locus_tag="VFV61_06925"
                     /inference="COORDINATES: protein motif:HMM:NF015944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEX4900249.1"
                     /translation="MTYKKVLLLTLVVFVSLSASGCGYNTLTTKQQNVKGKWANVETQ
                     LQRRADLINNLVESAKLAGVQEQEVFGKIAEARSRLLNAQQQAPQGEAGDKSPEQKQA
                     VIDAANSFGGTIGRLLVLQEAYPQLQSNQNFLKLQDEVAGTENRIATARKDYNDGVQD
                     YNTTRARFPTVISAKLFGFTEEPYFKAEEGATQPPTINANSLRQPTP"
     gene            complement(3918..4664)
                     /locus_tag="VFV61_06930"
     CDS             complement(3918..4664)
                     /locus_tag="VFV61_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900250.1"
                     /translation="MSKLVAQEALNGLIQDLRATHGDNLASVVLYGSAAAGDHVELRS
                     DYNLLISLHRITPADLRQAQAPMREWQRLGHPLPVYFTVEELSDAADVFPIEFHQMSQ
                     ARILLFGKDPFEFVKLSDVHLRHQAEYELRSKLIQLRRLYIPASISLTKLCDLMSDSL
                     ASFAALFRAVLLLHGAAAPVAKPDCVRATVRLLGLDDQPFEKIFALRAQGELPATEQE
                     ANDLFAAYMLQIERVVEAVDETNIAEQPLH"
     gene            complement(4746..>5255)
                     /locus_tag="VFV61_06935"
     CDS             complement(4746..>5255)
                     /locus_tag="VFV61_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900251.1"
                     /translation="LEGDLNDGLVGQIQRERLVPQLRKGNYSQGIYDTVQAYVATLAA
                     KRGFAIEGIDQRYAYREGREPSGIGSRSGGLSRNVCTLLIILVVIFLILSSMKRGGRG
                     GGGGCLNLLLLNSLLNSGGRGWGGSGSGWSGGGFGGGGWGGGGFGGGGFGGGFGGGGA
                     GGGGAGGGW"
BASE COUNT         1190 a         1454 c         1479 g         1132 t
ORIGIN      
        1 agccctcgcg gaacagttca acctgaactc agcgcaaatt cgcaaagacc tcggttattt
       61 tggcgagttt ggggtccgcg gcgttggtta ctacgtagcg gatctacgcg cccatcttac
      121 caagattctc ggcctggaca ccgcgcatcg cgtgggaata gtcggggctg gccgcctggg
      181 cacggcactg gccaactatc aaggcatcga gaaatcgaac ttcagcgtgg ttgcgttatt
      241 tgacaatgat tccgggaaga ttgggacgcg catcggtccc ggcaacgtac tcgtccacga
      301 cgtgaaacgc attgcgcgcg tagtcaagga caaaagtgtt gatgtcgctg tgattgccgt
      361 cccggcgcgg gccgcgcaac gggtgttgaa tcaagtcacc ggcgcgggcg taagagcggt
      421 actaaatttt gcccctgcat ctttaagtgc aaggctcgga gtaaaagtta agaccgttga
      481 cttaacgact tcactggaat cattatcgta tttcctctcg cgaccgcagg cgagcgtgac
      541 gaccaaggcc cgcagaggcg ccgaacctga agaactgccc gcgcacagcg aacgcgaact
      601 ataaggacga tacccgcgat caatggatga gcaaacagct cgacgcgaaa tagttcgcat
      661 cggccaactc atgtacgaac gcagttacgt tgtctcctct gacggcaacg taagtgtgcg
      721 tctcgacgac ggtcgcatcc tggccacgcc gacgatgacg tgcaagggtc gcatgacgga
      781 agactctttg gcgctgactg atgcaaccgg aaaagcactg agcaatcgca aagcctcaag
      841 cgagctggcg atgcacatcc tgatctacaa cgagcgaccc gatgttaagg cggtctgcca
      901 tgcccatccg ccgcacggga ccgcctttgc ggtagccgga ttggcgatcg accagccgat
      961 actttccgaa gtgattctga cattgggatg cgtgccgctg gccgcttacg gaacgccttc
     1021 tactgaggag ttaactgagg cgatgcgccc gcttgtgaag catcacaacg cacttctgat
     1081 ggcgaatcac ggtgcagtgg cctatggatc ggatctgtgg caggctttcg atcgtttaga
     1141 aaccctggaa cacacggcgc gcattgcgat cctttcgcgc atcttaggtg ggtcgaagaa
     1201 tttgccagtc gacgccattg agaagttgat caatgtccgc gaagcggcag gatatctcgg
     1261 cgagagtgct cgttgtcagg cgtgtggata tttgcacgaa acacagctga cgtgtcccac
     1321 aggcgaccga ccaccagttt cgggttatca tgagccagcg actaatggca gcgggaagat
     1381 tgcgttgacc agggaagaac tggttgagct attggcgcag gctgtaagtc gcgaatcgtg
     1441 aatcgttaac ttgctaacgc cgaagtttct aaatcggcaa ttgccaatcg gcaatcgtaa
     1501 attcttagaa ggagcataca aatgccacaa gaagcactcg gcatggtgga aacgaaagga
     1561 ctggtggcga tgattgaagc cgccgatgcg atggtgaaag ctgccaacgt aacactgatt
     1621 ggctacgaaa aaattggcgc gggctttgtc accgctatcg tgcgcggaga tgttgctgcg
     1681 gtaaaagctg ctaccgatgc tggcgctgcc gccgcacgtc gcgtgggcga actggtcagc
     1741 gtccatgtaa tcccgcgacc acacggcagc gttgacgatg cacttccgat tgggaatcgc
     1801 ggctagcatg attatcgccc gcattctcgg aaccgtcgtt gccacgcaaa aagacgagcg
     1861 cctgctggga aagaagcttt tgatagttcg tcctgttaac gtcgatggga cagacacaac
     1921 cgggtacgtt gtggctgttg atactgtggg cgccggtttc cacgaaaagg ttttggtagt
     1981 cgctggctcc agtgctcggc tggcgcaagg aatgaaagac gttcccgtcg atgccgctgt
     2041 ggtgggcgtg attgataccg tggatatcac ttcataagtt taaggttcca ggtttcaggt
     2101 tcgcttaatc cgaaactggc ttgccgcgga aaccgtgaac cttaaacatg ggatgtgaaa
     2161 cctgaaaccc attcatgcag ctcgctcgcg tcatcggaac tgttgtcgcc acggccaaga
     2221 atgaatcact tgagggccgt aagttgcttg ttgtgcaaac tctgaaccgg gatttgcaac
     2281 cctcgggtaa gcccctcgta gcagtagact cagtcggagc gggcctgggc gaattggttt
     2341 tctggtgtcg cggcaaggaa gcgagtttcc ctttcaaacg cgaagacacg cccacagact
     2401 gcaccattgt cggtatcgtt gattccgagt tccacgtaac gcaaagcgcc agttaccaga
     2461 aaaggatttc gtagtctaat gattctcgcg cgagtgctcg gaaacatcgt tgccacgcaa
     2521 aagaatcggc gctacgaaaa tgcgagcgtc atgctctgcc agcagatcac gcctgaaggt
     2581 gatgaaactt cgtacacggt gctggctcta aactcggttg atgctggtgt gggcgataca
     2641 gtcctgattg ttcaggaggg ttggagtgct tcgactgcgg cgacagggaa gcccggagcg
     2701 gcaatcgatt cggcaatcgt cggcgttgtc gatcacattg atttgttata ggaactgatg
     2761 agcacggatc aagatcaagc tcgggagata gcggaacgga tcgcgcggcg cgtctcaggt
     2821 agcagtttga gttcgagctc agccggccgc caacagggtt ccgaagttag ttcacagtta
     2881 gctgcagtgc gcgcggggat taatgacctg caaaggaaac tggcgcaact ggaagccgca
     2941 gtgagttccg ctccttccgc gccgaccgtc ccgcaaatgt ccccgaccca aggcgctatg
     3001 agtccccatc gctcaggtca cgtgactcac tctccctggc tggcgggcat caacagctct
     3061 tactcgcatc ccagtgaaga aaaatttgga gtggaagagg cgacagtctc agcactggtg
     3121 gactttttcg aaaaggaaaa gctttgcagc gtcgagccgg gcggcaagcc ctgcgaccac
     3181 tgctcgatgt gcagctccag aggattctga gtgcttttgg agtgcgccgt cgccgcttcg
     3241 ctctgccggc gcactccata aaaagactag ggcgtcggtt gtcgcagcga gttggcgtta
     3301 attgtcggcg gctgcgtcgc accttcttcg gccttgaagt agggctcttc cgtaaaacca
     3361 aaaagcttgg cagagatcac cgtgggaaaa cgtgcgcgcg tggtgttgta gtcctgaaca
     3421 ccgtcgttat agtccttgcg cgccgtggcg atacgatttt cagtccccgc tacctcgtcc
     3481 tgcaacttca gaaagttctg gttggactgc aactgcggat aggcctcttg aagtaccagc
     3541 aggcgcccaa tggtgcctcc aaaactgttg gcggcgtcaa tcacagcctg cttctgttcc
     3601 ggactcttgt cgcctgcctc accttgcggc gcctgctgct gcgcattcaa caatctcgag
     3661 cgcgcttccg cgatctttcc gaaaacttcc tgctcttgca cgcctgctaa cttggccgac
     3721 tcgacgagat tatttatcaa gtctgcgcgc cgctgcagct gagtctcgac attcgcccat
     3781 tttcctttga cgttctgttg tttggtagtc agggtgttat agccacaccc gctggcagac
     3841 agtgatacga atacgacgag cgttaacagt agaacttttt tgtaagtcat agtgattctc
     3901 cgcttaggtt gtttgactca gtgcaggggc tgctccgcga tattggtttc atcaacagct
     3961 tcgacgacgc gctcgatttg caacatgtaa gcagcgaaga ggtcattcgc ttcctgctcc
     4021 gttgccggca gttcgccttg cgctcgtagc gcgaagattt tttcaaaggg ctggtcatca
     4081 agccccagca ggcgcaccgt ggcgcgcacg cagtcaggct tggccaccgg cgctgccgca
     4141 ccgtgcagaa gcaaaacggc acgaaacaag gctgcgaaac tggccagact gtcgctcatc
     4201 aggtcacata gttttgtcag cgaaatcgat gcgggaatat aaagccggcg caactggatc
     4261 agtttgctgc gtagctcgta ttccgcctgg tggcgcaagt gaacgtctga aagtttcacg
     4321 aattcgaagg gatctttgcc gaagagcagg atgcgcgcct gggacatctg gtgaaattca
     4381 atggggaaaa catcagcggc gtcggacaac tcctcaacag tgaagtaaac gggcagcgga
     4441 tggcccagac gctgccattc cctcatcggc gcctgcgcct ggcgcaaatc ggcaggtgtt
     4501 attcggtgca gcgagatcag caggttgtaa tcggagcgca gttccacgtg atctccagcg
     4561 gctgcggatc cgtaaagcac tacggacgcg agattgtcgc catgcgtagc acgtaagtct
     4621 tgaatcaaac cgttaagcgc ttcctgagcg actagcttag acatttctat taagggtgtc
     4681 cctgcatact aacaaactca gttaccgacc agaaggaacc atcaagcagg cgaacagcgc
     4741 cgttcttacc aaccacctcc ggctccgcca ccgcccgcac ctccgccgcc gaacccgcca
     4801 ccaaaaccac caccaccaaa tccaccgcca ccccagccgc cgccgccaaa gccgcctccg
     4861 ctccagccac tacctgaccc gccccagccg cgtccaccgc tgttcagcaa cgaattcaaa
     4921 agcaacaaat tcaaacaacc accgccgccg ccgcgcccgc cgcgcttcat cgatgagaga
     4981 atcaaaaaga tgacaacgag aatgattagc agtgtgcaaa cgttacggga aaggccgccg
     5041 gatcggctgc ctataccgga cggttctcgt ccttcacgat atgcataccg ttgatcgatc
     5101 ccttcgatcg cgaaaccacg ctttgcggcc agggtagcga cataggcttg cacggtgtcg
     5161 taaataccct ggctgtagtt tcccttcctt aattgcggta ctagtcgttc gcgttgaatt
     5221 tgcccgacga ggccgtcatt gaggtcgcct tcaag
//
LOCUS       DASUEX010000410         2938 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1655662, whole genome shotgun sequence.
ACCESSION   DASUEX010000410 DASUEX010000000
VERSION     DASUEX010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2938
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1655662"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            189..632
                     /locus_tag="VFV61_06940"
     CDS             189..632
                     /locus_tag="VFV61_06940"
                     /inference="COORDINATES: protein motif:HMM:NF024954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX4900252.1"
                     /translation="MRQTELKLRQKDRQTLEEFRSKGNHMARELNRAHILAALDRKIP
                     ESQIREVLGVGRTAIWRTRGAYLGGGLAFALQDVPRPGKPKRYRTDEEAQVAALACSD
                     PPAGAKRWTVALLTEATRSHPNMGEISRETIRRFLKKTASNRGVS"
     gene            632..1291
                     /locus_tag="VFV61_06945"
     CDS             632..1291
                     /locus_tag="VFV61_06945"
                     /inference="COORDINATES: protein motif:HMM:NF033545.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEX4900253.1"
                     /translation="MWCIGRLTEEYRSRMYGLLDLYARPYLEREPVVCIDEKSKQLLK
                     DMRAPLPLKPGVLAKQDYEYKRAGTCNLFVAVEPKGQRRIVEVTARRTKVDFVDFVCR
                     LLKAVYARARKVHLVLDNLNTHFRSSFEEVLGHKTATVLLRRIEFHYTPKHASWLNMA
                     EIEIGILERQCLGRRLKDHGTLTLEVAAWQQRRNAHHCGIVWNFTRQDADRKLVRHYV
                     S"
     gene            1524..1961
                     /locus_tag="VFV61_06950"
     CDS             1524..1961
                     /locus_tag="VFV61_06950"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX4900254.1"
                     /translation="MSFRKILVAVDESVFAARAADVGFDLARSLGAEVAIIHVVDPSV
                     LAYAPEGGIPASELVTLAQQDGKRLLAAFGQRAALQPAPLEFSHVGKTATEIVNAAKD
                     WSADLIVVGSHGRGGVERLLLGSVAEAVIRHAACPVLVVRAEE"
     gene            2368..2832
                     /locus_tag="VFV61_06955"
     CDS             2368..2832
                     /locus_tag="VFV61_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900255.1"
                     /translation="MKKIGVSLVIAAIAVLFAAVSSNAQRGPGMMWRGSGGWGPGTQY
                     NRMYDPKTVETISGEVISIDQFTPAKGMIGGIHMNVKTGKETISVHLGPSWYLENQDV
                     KIEAKDRVEVKGSRIAFGGKPAMIAAEVRKGQEVLTLRDESGFPAWMAWRRR"
BASE COUNT          671 a          750 c          887 g          630 t
ORIGIN      
        1 cctgccataa caaataacta cttattgtgg cagaagacgc aaaggggtgt ccatccattg
       61 gtgacgatca acttggctcg gtacttatta gaatcaagat tgtggcagct gacgcaacct
      121 agtatcttga cacgttaata acgaaacatt acaatgacgc atcattttac caaggagatg
      181 tgtcagccat gcgccagacc gaattgaaat tgcgccaaaa ggaccgccag actctggaag
      241 agtttcgttc gaaaggtaac cacatggccc gtgaattaaa tcgcgcccac atcctcgctg
      301 ctctagatcg aaagatcccc gagagccaga tcagggaagt attgggggtg gggcgcacag
      361 cgatctggcg tacgcgtggc gcgtacttgg ggggcgggct cgcatttgcg ttgcaggacg
      421 tcccgcgtcc gggcaagccc aagcggtacc ggaccgacga ggaggcgcag gtggcggccc
      481 tggcctgttc ggatccgccc gcaggcgcca agcgctggac ggtggcgcta ctgaccgagg
      541 ccacgcggag ccatcccaat atgggtgaga tcagccgaga aaccattcgc cggtttctaa
      601 aaaaaactgc ctcaaaccgt ggcgtaagct gatgtggtgt attggccgtc tgaccgagga
      661 atatcgcagc cgcatgtatg gcctgctcga cctctacgct cggccctatc tggagcggga
      721 accggtggta tgcattgatg agaagagcaa gcaactgctc aaggacatgc gcgccccgtt
      781 gcccctcaag cccggggtgc tggccaaaca ggattacgag tacaaacggg cgggtacctg
      841 caacctcttc gtggccgtcg aacccaaggg ccaacggcga atcgtggaag tgactgcgcg
      901 tcgcacgaag gtggactttg tggacttcgt ctgccgactg ctgaaagcag tttacgcgag
      961 agccaggaag gttcacttgg ttcttgacaa cctcaacacg cattttcgct ccagctttga
     1021 ggaggtgctc ggtcacaaga cagccacggt gctgttgcgc cgcatcgagt ttcactatac
     1081 gcccaagcat gcgagttggc tcaatatggc cgaaatcgag atcggcatcc tggagcgcca
     1141 gtgtctgggg cggcggctca aggaccacgg aacgctcacg ctcgaagttg ctgcttggca
     1201 gcagcgacgc aatgcccacc attgtggcat cgtgtggaac ttcactcgcc aagatgctga
     1261 tcggaaattg gttcggcatt atgtttcgta attaatgagt ttcaatacta gtgtagtgtc
     1321 tcttaaatcg gaagacttat ttgcgcgtca ttccggccaa gctggcgata gctagcgcga
     1381 gccggaatcc aggacttcga aggaccgctg gattcccgct tgcgcgggaa tgacgactaa
     1441 ggccgtcttc gataaagtac aaacagcgtg agacactaca ctagcaaccg cgggaagcat
     1501 cgttagatcc gaggagattt ctcatgagtt ttcggaaaat tcttgttgcg gtggatgaga
     1561 gcgtgtttgc ggcgcgcgct gccgatgtcg ggttcgatct ggcgcgttcg ctcggggctg
     1621 aggtggcgat aattcatgtc gtcgatccct ccgttcttgc ttatgctccc gagggtggga
     1681 ttccggcaag cgaactcgtc actctggctc agcaggatgg caaaagactg cttgccgcat
     1741 tcggtcagcg tgccgcgttg cagccggcgc ctcttgagtt cagtcatgtc ggcaagactg
     1801 cgaccgagat cgtgaacgca gctaaagact ggtccgcgga cctcatcgtc gtcggcagtc
     1861 atggacgcgg gggagtcgag cgcctgctgt tgggcagcgt cgccgaggcg gtgatacgcc
     1921 acgccgcatg tccggtgcta gtcgttcgcg cagaagagtg aacccgctcg cggcgaccag
     1981 attctcgtcg gtcccgagac ggtcagacgg gtgcaaggtc attttcttct gcgcctcatc
     2041 ggtcccgtcg agctcaaaaa catcggccat ccggttgagg tctgggaagt gatggcggcg
     2101 ttgcaattcg accgcggcgc taaaacaggt taaatctatt ctcgcctact ttgatcaaaa
     2161 tgcgcccgca atttcggttc acgtctgctg aaaaaattcg aaataagatt ttccattact
     2221 gcaaatagat catctatagt ttcgcaatcc tgaagatatt atcaccgcgt ggggcgagaa
     2281 attagagatt cccccgtggc ataggcattg ctcctctgac tcagtgattc gttagatgat
     2341 cgaaactact cagaaggggg actaacaatg aagaagatag gtgtaagttt ggtgattgcc
     2401 gcaattgcgg tgctgtttgc ggcggtttct tcaaacgctc aacgagggcc gggaatgatg
     2461 tggcgaggca gcggcggctg ggggccgggc acgcaataca atcggatgta cgatcccaag
     2521 accgtggaga ccatcagcgg tgaagtgatc agcatcgatc agtttacccc agcgaagggg
     2581 atgatcggcg gtattcacat gaacgtgaaa accggcaaag agacgatctc cgtgcatctc
     2641 ggtccgagct ggtacctgga gaaccaggac gtgaagatcg aagcgaagga cagagtcgaa
     2701 gtaaagggtt cgcggattgc gttcggtggt aagccggcca tgatcgctgc cgaagtgaga
     2761 aagggacagg aggtgttgac gttgcgcgat gaatcaggtt tcccagcctg gatggcgtgg
     2821 cggcggcgtt aggtttacgg gtgtcagact cgctaataaa ctcggtagag aacctgctac
     2881 gtctgttttc tccatcccct catctgggat ctgatagacg ggtgagggga aagctaac
//
LOCUS       DASUEX010000411         3310 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1655906, whole genome shotgun sequence.
ACCESSION   DASUEX010000411 DASUEX010000000
VERSION     DASUEX010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3310
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1655906"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..1091)
                     /locus_tag="VFV61_06960"
     CDS             complement(69..1091)
                     /locus_tag="VFV61_06960"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX4900256.1"
                     /translation="MSSPQMQQPNPALLFQTINAYQRTEAMKAAIELEVFTAIGEGKQ
                     TAAEIAQRCGAAERGIRILCDFLTIMGFLTKAEKNYSLTADSAMFLDKKSPAYMGGAT
                     KFLLSPMLTAGYKEFTAAVRKGGVAASEEGTIAPDHPVWVDFARAMSPLMAMPAELMA
                     RLVDPEAKQKLKILDIAAGHGFFGIAFARNNPNVEVVALDWANVLEVAKENAAKAGVA
                     DRYSTIPGSAFDVDYGAAYDLVLLTNFLHHFDPPTCEKLLRKVHAALKPGGRAVTLEF
                     VPNEDRISPPESASFAVMMLGSTPAGDAYTFAELEQMMTAAGFGKSEFHQLPPSIQQV
                     VISHKD"
     gene            complement(1110..1622)
                     /locus_tag="VFV61_06965"
     CDS             complement(1110..1622)
                     /locus_tag="VFV61_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900257.1"
                     /translation="MIGSNKFHLPMAALFLLALAGGSLAKPWRGIVPLHTTRAQVRKL
                     LGKPIIGGEGSIDLYDLREGRIHIMYARHPCEVGLPADWGNWRVPRDTVVNISIHLHE
                     EIPLQRLHIRNIEKYKWYTGESGATYYHDRIRGLEYQVQEGMVTAITYGPAARDRALR
                     CKRHTPLIRY"
     gene            complement(1932..>3310)
                     /locus_tag="VFV61_06970"
     CDS             complement(1932..>3310)
                     /locus_tag="VFV61_06970"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX4900258.1"
                     /translation="PGENNSLALPTQENSMIDSSSSSRSGNSRRDFLSLAGKGLGLAA
                     LSSPLVSSLVKEVYAAGDAVAHLTPQQAAMDEDYWSVIQNAFTVTRGIVNLNNGGVSP
                     SPRIVTEALVRYIWQQEDATAYTMWQILEPQSETIRTGLAELFDCDREEIAITRNASE
                     SLEILLMGMDFKPGDEILTTTQDYPRMLTTLRQREKREGLALKLIKIPIPPQNMSEIS
                     TAFERGITNRTRLILISHQVNITGQITPVKAVCEMARAKGIETIVDGAHSFAQFHFTQ
                     KDLGCDYFGTSLHKWLYAPKGTGLLYVKRDKIAKLWPLMAAEEKNASDIRKFEEIGTH
                     SAAPRLAIGEAMLFHNGIGGKRKEARLRYLSRYWMNRLKDVPKVRFNTSFDPAQSCAI
                     ANVQIEGINPSAIGSYLFAQHHIFTTPIIHDEFQGIRITPNVYTTLGELDRFCNIMEM
                     IARKGLPV"
BASE COUNT          758 a          895 c          891 g          766 t
ORIGIN      
        1 aaaaaagcga tccacgaagt ctcattgaat tcgtggatcg ctttctagca aacctgccgg
       61 aagtctttct agtctttatg tgaaatcacc acttgctgaa tcgacggcgg cagctgatga
      121 aactcactct tgccgaagcc cgcagctgtc atcatctgct ccagctcagc aaacgtgtac
      181 gcgtcacccg ccggggtgct gcctaacatc attaccgcaa acgaggcaga ctcaggcgga
      241 gaaatgcgat cttcgttcgg tacgaactcg agcgttacgg cacggccacc gggctttagt
      301 gccgcatgaa ccttgcgcaa aagcttttca caggttggtg gatcaaagtg gtgcaggaaa
      361 ttagtcaaca gtaccaggtc gtaagcagcg ccataatcca cgtcaaacgc gcttccggga
      421 attgtgctgt aacgatcagc gactccggct ttggctgcat tctctttggc aacctcgagc
      481 acgttggccc aatctagcgc aaccacttcc acattgggat tgttccgggc gaaggcaatt
      541 ccaaaaaagc cgtgtccggc agcgatatca agaatcttca atttctgttt ggcctcaggg
      601 tcaaccagac gggccattaa ctcagccggc attgccatca gcggcgacat cgcgcgcgcg
      661 aaatcaaccc aaaccggatg atccggggcg attgtgccct cttccgaagc agcaactcct
      721 cccttgcgaa ccgcggcagt aaactctttg tagcccgcag tcagcatcgg cgagagcagg
      781 aacttcgtgg cgccgcccat gtatgccggc gacttcttgt cgagaaacat ggccgagtcg
      841 gcggtcaggg agtaattctt ttcggctttc gtcagaaagc ccatgatggt gagaaagtca
      901 caaaggatgc gaatgcctcg ttcggcggcg ccgcagcgtt gtgcgatttc ggcggctgtc
      961 tgttttcctt cgccaatggc cgtgaagact tccaattcaa ttgccgcctt catggcttcg
     1021 gtgcgctggt aggcattgat tgtttgaaac agaagagcgg gatttggttg ttgcatttgt
     1081 ggactagaca tttcatctcc tggaaagttt tagtaacgga ttaatggtgt atgtcgcttg
     1141 cagcgcagcg cgcgatcgcg cgccgcggga ccataggtga tggcggtgac cattccttcc
     1201 tgaacttgat actccaaacc acgaattcga tcgtgatagt aagtggcccc agactctccc
     1261 gtgtaccact tgtacttctc gatgttgcgg atatgcaggc gctggagcgg aatctcttca
     1321 tgcaagtgaa tgctgatgtt aacaactgta tcacgcggca cgcgccagtt tccccaatca
     1381 gctggcagcc caacttcaca cggatgccgg gcgtacatta tgtgaatgcg tccttcgcgc
     1441 aggtcataga gatcgatcga gccttcaccg ccgatgatcg gtttacccag cagctttctt
     1501 acctgcgcgc gcgtcgtgtg cagcggcact atgccgcgcc agggcttcgc aagggagccg
     1561 ccagcgagag cgagcaggaa aagcgcggcc attggcaaat gaaatttgtt ggaaccgatc
     1621 attgcgcacc tgagtacgac taggcacaaa aagtcttgag caattcttat tcacacctgg
     1681 cttaagccag gtgcatgcag gtcttacaga atagggaacc gtttaaacga caataatata
     1741 cttgactcaa cttcagcgga atcaaagaca cgtctgagag tgccaagtga ggtccgcagg
     1801 actgtcatca aatagccacg gccgtgaggc cgtggtcggt cgaaatctga atagtcgagc
     1861 gccgaagctg cgacagtaaa cccctatcac agtggtcttt tgtcaccgac acctcatacc
     1921 cgacacctgc cctacaccgg cagcccttta cgggcgatca tttccatgat gttacagaag
     1981 cgatcgagtt cgccgagcgt ggtgtagaca ttgggcgtga tgcgaatgcc ctgaaactcg
     2041 tcatgaataa tcggggtcgt aaagatgtga tgctgggcaa acagataact accaattgcc
     2101 gaaggattga tgccctcgat ctgcacgttg gcgattgcgc acgactgtgc tggatcaaac
     2161 gaagtattaa agcgcacctt cggcacgtcc ttgagccggt tcatccagta acgtgaaaga
     2221 taccgcaagc gggcttcttt gcgtttgccg ccgatgccgt tgtgaaacaa cattgcctcg
     2281 ccgatggcca aacgcggcgc ggctgaatgc gtgccaatct cctcaaactt ccgaatgtca
     2341 gaagcgtttt tctcttccgc cgccatcaag ggccacagct tggcgatctt atcgcgcttc
     2401 acataaagca gcccggttcc cttcggcgca taaagccact tatgcaaact cgtgccgaag
     2461 taatcacagc ccaggtcctt ttgcgtgaag tggaactgcg cgaatgaatg agcgccgtca
     2521 actatcgtct caatgccttt ggcgcgggcc atctcgcaga ctgcttttac gggcgtgatc
     2581 tgtccggtga tattcacctg atgtgagatc aagatcagcc gtgtccgatt cgtaattcct
     2641 cgttcaaaag ccgtcgagat ctcactcatg ttttgcggag gaatcggaat cttgatcaac
     2701 ttcagggcta agccttcgcg tttctcacgt tgccggaggg tagttaacat gcgcgggtaa
     2761 tcctgagtcg tcgtcagaat ttcgtcgccg ggtttgaaat ccatgcccat cagcagaatt
     2821 tccagcgact cggaggcgtt acgggtgatg gcaatctctt cgcggtcgca gtcgaaaagt
     2881 tctgccagcc cggtacgaat tgtttccgat tgcggctcga ggatttgcca catcgtgtaa
     2941 gccgtggcgt cttcctgttg ccagatataa cgaaccaggg cttctgtaac tatgcgcggg
     3001 ctgggcgaga cgccgccgtt gttgaggttg acgatcccgc gggtaacggt gaacgcattc
     3061 tggatcactg accagtagtc ttcatccatt gccgcttgtt gcggagtgag gtgcgcgacg
     3121 gcatcgccgg ctgcatagac ctctttcacc aaagaactaa cgagtggcga cgacaatgca
     3181 gcgaggccga gaccttttcc tgccagagac aggaagtcgc gacgcgaatt gcccgagcgg
     3241 gaagacgatg acgaatcaat catggaattc tcctgtgtag gtaacgccaa gctgttattc
     3301 tcaccgggct
//
LOCUS       DASUEX010000412         2057 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_750299, whole genome shotgun sequence.
ACCESSION   DASUEX010000412 DASUEX010000000
VERSION     DASUEX010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_750299"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="VFV61_06975"
     CDS             complement(<1..205)
                     /locus_tag="VFV61_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900259.1"
                     /translation="MIVNGSIMITGSRDVARETARVLFEQHLSPFLSQGRTWLVGTAH
                     GVDEWALEWLLENSETCWTVVPYT"
     gene            complement(342..>2057)
                     /locus_tag="VFV61_06980"
     CDS             complement(342..>2057)
                     /locus_tag="VFV61_06980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900260.1"
                     /translation="AVGSMLIGYQSIALAQTDSALPNHVHYTKSADQDKPSATGALAP
                     RLQKLGNHTFPVSTKNRQAQLFMNQGLNLSYAFNHAEAGRAYREAARLDPNLAMAYWG
                     EALALGPNINAPMSPDNEPKAVEVINKAVALKSKASPRERALIEALTHRYSGRAEDRR
                     ARDVAYADAMRKVHLQFPNDQDIAMLYVESVMDLRPWGYWTRDGTPYDRTAEIVALTE
                     KVIAQNPNHPGALHLYIHLMEAYAAFKAEPAADRLLTLMPAAGHMVHMPAHIYQRVGR
                     YADAARSNELAIAADEDYISQCRAQGLYPMAYYPHNLHFLWFAATADGRSQMAIQAAR
                     KAASRVDDETLKAVPLLAGFRIVPYFALTRFGKWDEMLREPEPPAFSAYMRGQWHYAR
                     GTAFLGKGQTKEAEQELAKLKELMDDKSLDQPLFSPNTGHAILSIAEEVLAGEIAAAK
                     QDYDEGINHLERAVRLEDALVYTEPAEFHYPPRQALGSVLLAANRAPEAETVYWEDLR
                     RNRDNGWSLFGLMQALKAQGKNDDAALVEARFKKAWARADIVLSNSRFGSQMSAQATT
                     GSPQK"
BASE COUNT          416 a          582 c          599 g          460 t
ORIGIN      
        1 gcgtgtaggg aacgacagtc caacaagtct cactgttttc gagaagccat tcgagggccc
       61 actcgtcgac cccatgtgcg gttccaacga gccaggttct accttgagag aggaatggtg
      121 agaggtgttg ttcgaacaat acgcgcgccg tctcgcgagc gacatcgcgc gaaccagtga
      181 tcattattga cccgttgacg atcatggcct ttgctactcc ggaacccaga ctgtcagttt
      241 ccgttagata taagaaagga cagccggaac ggctgccctt acttaattag agcggtgagc
      301 cgagcaggcc tcatacctgc gacccaccag ttaaagtcac cctatttttg tggtgatccc
      361 gttgttgctt gagcactcat ctggctcccg aagcgagagt tgctgagaac gatatccgcg
      421 cgtgcccagg ctttcttgaa ccgcgcttct actaaagcgg cgtcgtcgtt cttgccctgc
      481 gctttcaacg cctgcatcaa gccgaacaga gaccacccgt tatcgcgatt gcggcgcaga
      541 tcctcccagt aaaccgtttc cgcttctgga gcgcggttcg cggcgagcaa caccgaaccg
      601 agcgcctgac gtggtggata atgaaactcc gcaggctctg tataaaccaa agcgtcttcc
      661 aacctcaccg cacgttcgag atggttgata ccctcatcgt aatcctgttt cgcagcagca
      721 atctcgcccg ctagcacttc ctccgcgatc gagaggatcg catggccggt gttcggcgag
      781 aacaacggtt ggtcgagcga cttatcgtcc atgagttctt tcaacttcgc gagttcctgc
      841 tcggcttctt tagtctgacc cttgccgagg aacgccgtgc cgcgcgcgta atgccattga
      901 ccgcgcatgt aggcgctgaa tgccggtggt tcaggttcac gcaacatctc gtcccattta
      961 ccaaaacgcg tcaaggcaaa gtacggcact atgcggaaac ccgcgagaag cggaaccgct
     1021 ttcaaagtct catcgtcaac tcgcgaagcc gctttgcgtg ctgcctggat cgccatctgg
     1081 ctgcggccgt cagcggtggc ggcgaaccag aggaagtgca ggttgtgtgg atagtaggcc
     1141 atcggataga ggccctgcgc gcgacattgc gagatgtaat cttcatcagc ggcaatcgcc
     1201 agctcgttgc tacgggcggc gtcggcgtag cggcccacgc gttgatagat atgtgcgggc
     1261 atgtgcacca tgtgacctgc ggcgggcatg agcgtcagca ggcgatcggc ggccggctca
     1321 gctttgaatg ccgcgtatgc ttccatcaga tgaatgtaaa gatgcagcgc gcccggatgg
     1381 ttcggattct gggcgatcac tttctctgtt agggcgacga tttctgcagt gcgatcataa
     1441 ggcgtgccgt cgcgcgtcca gtagccccag ggtcgcaaat ccatcacgga ttcaacgtag
     1501 agcatcgcga tgtcctggtc gttcgggaat tgaagatgca ctttgcgcat cgcgtcggcg
     1561 taagccacgt cgcgtgcccg gcgatcttca gcacggcctg aatagcgatg cgtcagcgcc
     1621 tcgatcagcg ctcgctcgcg cggcgaagct tttgatttga gcgcgacggc tttgttgatg
     1681 acctccacgg ctttcggctc gttgtcaggg ctcatcgggg cattgatatt cgggcccagc
     1741 gcgagtgctt cgccccaata cgccatcgcc agatttggat ccagtcgcgc agcttcacgg
     1801 tatgcgcggc cggcttccgc gtgattgaag gcgtaggaaa gattcaatcc ctggttcata
     1861 aaaagctgag cttggcgatt cttggtggaa acgggaaagg tgtgattgcc aagtttctga
     1921 agacgaggcg ccaaggctcc agtggctgac ggcttgtcct ggtcggccga ctttgtgtag
     1981 tgaacgtgat ttggaagtgc tgagtcggtc tgggctaacg caatactctg atacccgatc
     2041 aacatcgacc cgacggc
//
LOCUS       DASUEX010000413         6079 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_995919, whole genome shotgun sequence.
ACCESSION   DASUEX010000413 DASUEX010000000
VERSION     DASUEX010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6079
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_995919"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            179..1756
                     /locus_tag="VFV61_06985"
     CDS             179..1756
                     /locus_tag="VFV61_06985"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX4900261.1"
                     /translation="MIFSSPHPTVAIPEISLTEFVLQQVAEFGDKPAIIEGQTGRTIT
                     YAQLVPLIRRLAAGLAGLGLKKGDVLAIYSPNLPEFALTFHAVASLGGVVTMVPPLFT
                     DDEIKTQLRDSAAKFLVTVPGLIGKAREAATAAGIETLIVIGDTGSDAEIPFASLLAH
                     NAEPPAVLINPREDLAALPYSSGTTGFPKGVMLTHRNLVAMLCQIQANEVLTRDDTMI
                     CIVPMYHLYGLHVVMNLALSQGATVVTLPRYDLKQFLEVLQKYQVTIAPLVPPLVLAL
                     SRAPEVAEYDLSKLRLIHCGAASLAPNIAAECSERLGCRIKYGYGLTEVSPLSHASAS
                     LEVDKAGSVGYCLPNTECKIVDPETNAELLANEEGEIWIRGPQVMKGYLGNLQATEEM
                     IDEEGWLRSGDVGYADEQGRLFVVDRLKELIKYKGRQVAPAELEAVLLSHPAVADAGV
                     IPSPDEEAGEVPKAFVVLKGRASAEELIEFVAQRVAPHKRIRLVEFVREIPKSPAGKI
                     LRRLLVQRERARTQSSA"
     gene            complement(1753..2664)
                     /locus_tag="VFV61_06990"
     CDS             complement(1753..2664)
                     /locus_tag="VFV61_06990"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX4900262.1"
                     /translation="MPPYRIMTFDGGGVKGSLMATLVKRICDKFPNLLEQVDLFAGTS
                     TGSVVALTLASGFNADTLVSFYSQDNLRSAFSPSRFNWFRPKFSNANFRSLLESHFPG
                     NPRLRDLTKHNLVAPTFKLDNKKKQDWCPVFAHNFPGSHHLDELVVDVALRSAAAPTV
                     FPSHQGHIDGGMVANNPSTVAIAMALGHKTPRPALEDIRVLSIGTGLTPTIISLDTTN
                     WGIVQWLLNPFRKPQTPLLNILFDGVVEADASMSKHLLRERYIRVNPKLAKQTELDDW
                     KAIPQLIQTANDYDLAPAIDWIEKHWN"
     gene            complement(2687..4264)
                     /locus_tag="VFV61_06995"
     CDS             complement(2687..4264)
                     /locus_tag="VFV61_06995"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX4900263.1"
                     /translation="MQQRIIANRNLKAALPRKRVLIVNCYFDDSRQPIRRTSKIPQAV
                     GPIYLAGAFARELCDVSCYTEVASGPLEDEKLLSGLDMLVLTGLTNSFDRMLHLTAYA
                     RTKNPKVIVVAGGPPVRSLPLLARKFFDYTCMGDIEELCDVIKETFGAAYVADEMLPR
                     YDLAYWLGRIGYVETTRYCNFRCSFCSLTAEGHSYKTYDLEYIRRQILASGKRNRMFF
                     LDNNFYGSDRDHFHARLDLINEMRAQGQFQHWGALVTNDFFLRQENLSRIRAAGCELL
                     FSGLESFDNDWLRNFNKLQNTTAPQVEMITKSLNAGVVFSYGLMVDVTTRSVSDLRRE
                     LRFITRTPEITLPSFVTLSIPLLGTPYFFECLNRGSILPDTKLRDMDGTTVLQKPLDP
                     MSEVVKFVDDVQSFKGYRQQAIKHAAGFTRIYHSKLNTMQMILALGAGLMVCAQPLTG
                     SLAGLGWIRHRPKPRTYVSSTEPLDHMYTPAFRVESRYESYFKATMVTDKGGALHQDL
                     LESGLLRNAPRTAVAGA"
     gene            complement(4558..5139)
                     /locus_tag="VFV61_07000"
     CDS             complement(4558..5139)
                     /locus_tag="VFV61_07000"
                     /inference="COORDINATES: protein motif:HMM:NF020906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1990 domain-containing protein"
                     /protein_id="HEX4900264.1"
                     /translation="MFLLTKPSAERVRALIAAQWELPFSYPEVGATRTNPPQGYTVDH
                     NRVKLGAGKECFELAVKAFRSWKQFDLGWVQALPADTPIEVNAAVGVLARHLGFWSFN
                     VSRVVYVINEDGPIEKFGFAYGTLTTHVERGEERFTVEWHRDDDSVWYDILAFSRPNR
                     MIVNFGSPYARRLQRRFARESLKAMLQAVNSLS"
     gene            complement(5277..>6079)
                     /locus_tag="VFV61_07005"
     CDS             complement(5277..>6079)
                     /locus_tag="VFV61_07005"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific tyrosine recombinase"
                     /protein_id="HEX4900265.1"
                     /translation="DLARLQLWADKAGKPLDQLTRADLRKWIANLSRAGLAPSSVARA
                     VSAARGLFRFLMLDGHIKRHPTEDLDTPQRFAYLPQFLTEDEINQLLAAPDIATAEGV
                     RDRALIEVMYAAGLRVSELISLKQADVDVQSGLLICHGKGSKERRVPIGKSAVHWLQQ
                     YSAVRAGYGKPTSPQLFLNRGKPITRQFAWALIKRYASKAGVKDISPHTLRHSFATHL
                     LQHGADSRSVQALLGHSDISTTQIYTHITDRHLRSAYDKHHPRARRKA"
BASE COUNT         1386 a         1629 c         1716 g         1348 t
ORIGIN      
        1 gattggcgat tgccgatttg gtcagtagtc agtagtcagt agttagttgc tagtgggtgt
       61 tgctcccgta gcgaataccc aaatggaaaa tggaaaatga gaaacgctct ctgactgctg
      121 accactgacc actgactaca aacactgacc ccctacgctc tcgcaaggag actctttaat
      181 gatcttcagc agtcctcacc ccactgtcgc gattccggaa atctctctaa cggaatttgt
      241 tttgcaacaa gtcgcggagt ttggcgacaa gccggcgatt atcgaaggcc agaccggccg
      301 caccatcacc tacgcgcaac tcgttcccct gattcgccgc ctggcagcag gactagccgg
      361 gctcggtttg aaaaaaggtg atgtgttggc gatctatagt cctaacctgc ctgaattcgc
      421 cctcaccttt catgctgtcg cttcgttagg aggcgtggtc acgatggtgc cacccctctt
      481 taccgacgat gagatcaaaa ctcagttacg agattcagcg gcaaagttct tagtgacggt
      541 ccccgggctg atcggcaaag cccgtgaggc agctacagcc gccggcatcg agaccctaat
      601 cgtcattggc gatactggaa gtgatgccga gatcccgttc gcttcgttgt tggcgcacaa
      661 cgccgagccg cctgccgtgc tcatcaatcc gcgagaagat ctcgcagcct tgccctactc
      721 cagcgggacg actggtttcc ccaagggcgt catgctcact catcgcaatc tggttgccat
      781 gctctgccag atccaggcca acgaggtcct gaccagggac gacacgatga tttgcatcgt
      841 gcccatgtac cacctttatg gattacatgt cgtaatgaac ctggcgctgt ctcagggcgc
      901 cacagtcgtg acgctgccgc gctacgacct gaaacagttt ctcgaggtgc tgcagaagta
      961 ccaagtgacg attgctccgc tcgtgccacc tctggtgctg gcactctccc gagcgccgga
     1021 ggttgccgag tacgatctct caaagctgcg attgattcac tgcggcgccg cttcgctcgc
     1081 tcccaatatc gccgccgagt gctctgagag gctcggttgt cggatcaagt atggatatgg
     1141 actgaccgag gtcagtcctt tatcgcacgc cagcgcatct ctggaagtgg ataaggccgg
     1201 atcggttggt tactgtctac cgaacacgga atgcaagatc gtcgatccgg agacgaacgc
     1261 agaattgctt gctaacgagg aaggcgaaat atggatccgc gggccgcaag taatgaaagg
     1321 gtatttgggt aacttgcagg caactgaaga aatgatcgac gaggaaggct ggttgcgcag
     1381 tggtgatgtt ggttacgcgg acgagcaggg ccgtttgttt gtggtcgatc gcctgaagga
     1441 attaatcaaa tacaaaggta gacaggtagc cccggcagaa ctcgaggcag tcttactttc
     1501 gcatccggcc gtcgccgatg ccggtgtgat tcccagccct gacgaggaag cgggtgaggt
     1561 acccaaagct ttcgtggtgc ttaaaggcag agccagcgct gaagagctta tcgagtttgt
     1621 cgcccaacga gtggcgccgc acaagcgcat ccgcctggtt gaattcgtta gggagatccc
     1681 caagagtcca gccggaaaga tcctacgccg gctgcttgtt caacgtgaac gagctcgcac
     1741 gcagtccagc gcctaattcc agtgtttctc aatccaatct atcgccggag ccagatcgta
     1801 atcgtttgcg gtttggatta actgaggaat cgccttccag tcatcgagtt cggtttgctt
     1861 cgccagcttc gggtttacgc ggatgtagcg ctccctcaac aaatgcttgc tcatcgacgc
     1921 gtcagcttcg accaccccgt caaaaagaat gttcagaagt ggagtttgag gtttccggaa
     1981 cggattgagt aaccactgaa cgatgcccca attcgtcgtg tccagactta tgatcgtcgg
     2041 ggtcagtccc gttccgatag ataacacacg gatatcctca agcgcagggc gcggagtttt
     2101 atgaccaagc gccatcgcga tcgccacggt gctgggatta ttcgccacca tgccgccatc
     2161 aatgtgtccc tggtgagagg gaaagaccgt cggcgctgcg gcgcttcgca gtgccacgtc
     2221 gactaccaac tcatcgaggt gatgggaacc gggaaagtta tgagcaaaga ccgggcacca
     2281 atcctgcttc tttttattgt ccagcttgaa agtcggagcc accagattgt gcttggttaa
     2341 atcccggagg cgcgggttgc ccgggaaatg gctttcaagc aaagaacgaa aattggcatt
     2401 gctgaatttt ggccgaaacc aattgaaacg cgaaggcgag aacgccgagc gcaggttgtc
     2461 ctgagaataa aaactaacca gcgtgtcggc gttaaaaccc gaagccagcg tgagggccac
     2521 aactgagccc gtggaggtgc cggcaaacag atcgacctgt tccagtaagt tagggaattt
     2581 atcgcaaatg cgtttcacga gggtggccat caaggaacct ttcacaccgc caccgtcaaa
     2641 cgtcatgatt ctgtaggggg gcattgagaa tctcctaagt agtggttcag gcgcctgcta
     2701 ccgcagtgcg cggagcgtta cgtagcaggc ccgactcgag tagatcttgg tgcagtgcgc
     2761 cacctttgtc cgtaaccatg gtggctttga agtagctctc gtagcgtgat tcaacacgaa
     2821 aagccggcgt gtacatatga tcgagcggtt cagtgctgct gacgtaggta cggggcttcg
     2881 ggcgatggcg gatccaacct aagcccgcca gtgagccagt cagtggttga gcgcaaacca
     2941 tcaaacctgc tcccaacgcc aaaatcatct gcatggtgtt gagctttgaa tgatagatcc
     3001 gggtaaagcc agctgcatgt ttgatggcct gttgccggta acccttaaaa gactgcacat
     3061 catcaacaaa cttcacgacc tcactcattg gatccagcgg cttttgcaac actgtcgtac
     3121 cgtccatgtc gcgcagtttc gtatccggca aaatcgaacc gcgattgagg cattcgaaaa
     3181 agtagggcgt acccaacagt ggaatcgaaa gcgtgacaaa ggatggcagc gtaatttctg
     3241 gcgtgcgggt gatgaatctt agttcacgcc gcagatcaga aacgctgcgg gtggtgacgt
     3301 cgaccatgag gccataggag aagacgaccc cagcgttgag cgacttggta atcatctcga
     3361 cctgcggggc agtcgtgttt tgcagtttat taaagttgcg taaccaatcg ttatcgaacg
     3421 actcgaggcc gctaaaaaga agttcacagc cggctgctct tatgcgactc agattctcct
     3481 ggcgcaggaa gaagtcgttg gtgacgagcg cgccccaatg ctgaaactga ccctgggcgc
     3541 gcatctcgtt gattagatca agtcgggcat gaaaatgatc ccgatcgctg ccgtagaaat
     3601 tgttgtcgag gaaaaacatc cgattgcgtt ttcctgaggc cagaatctgg cggcgaatgt
     3661 attccaggtc gtaggtttta taactgtggc cttcagcagt gagggaacag aaagagcagc
     3721 gaaaattgca gtaacgagta gtttcgacgt agccaatccg tcccagccag taggccagat
     3781 cataacgcgg caacatttca tctgcgacat aggcggcgcc aaaggtttct ttgatcacgt
     3841 cgcagagttc ttcaatgtcg cccatgcagg tgtaatcaaa aaatttgcgt gcgaggagcg
     3901 gcagtgaacg cactggcggg ccaccagcga ccacaatgac tttgggattc ttcgtccgcg
     3961 cataggctgt gaggtgcagc atgcgatcga agctgtttgt cagtccggtg agtacgagca
     4021 tgtcgagccc ggaaagcagc ttctcatctt ccaacggacc actggcgact tcggtataac
     4081 agctgacgtc acagagctca cgcgcgaaag cacccgccag gtagattggc ccgaccgcct
     4141 ggggaatctt cgaagtgcga cgaattggct gtcgcgagtc gtcgaagtaa caattgacga
     4201 tgagcacacg tttgcgcggc agggcagcct tgaggtttct gttagcaatt attctttgct
     4261 gcattagcta tctcctgttg cgactacctg tcctgcccct gtcacgcgtt cgtgccccga
     4321 gaaatagaat ggtgtggctg tggcggcctc gaagggcaat ctggcgaggc gagtcggttg
     4381 gaaatcagcg atcagcgatc gggcgctact ctactctctc gataggcgtt ccgcaatatt
     4441 ttttgcattt atggccgcgg acgaagtctt gcccctcgcc cgaggcaagc tgggaccaaa
     4501 tccgcgtttc atctagcagt ttcttgggac tagcggcgag agctttacgg aaactgatca
     4561 tgataaagaa ttgaccgctt gcagcattgc ttttaaggat tctcgcgcga aacgtcgctg
     4621 cagcctacgc gcatatggcg aaccaaagtt gacgatcatc ctgttaggtc gcgagaaggc
     4681 aaggatgtca taccagacgg aatcgtcgtc acgatgccat tcgaccgtga agcgctcttc
     4741 accacgctct acgtgagttg tcagcgtccc gtaggcaaaa ccaaacttct cgatcggccc
     4801 gtcctcgtta atcacataga caacgcgtga gacgttaaaa gaccagaagc cgaggtggcg
     4861 cgcgaggacg ccgacagcgg cgttgacttc aataggggtg tccgcgggta atgcctggac
     4921 ccagccaagg tcaaattgtt tccaactgcg aaatgctttc acagcaagct cgaagcattc
     4981 cttcccagct cctaacttca cgcgattatg gtcaaccgta taaccctgcg gcgggttggt
     5041 gcgtgtcgct ccgacttccg gataggagaa cggcaactcc cattgtgctg caatcaaagc
     5101 gcgaactcgt tccgccgaag gttttgttag aaggaacatg tgagccaagt gggtttcggg
     5161 tatcgggtgc cgggtactgg gggtcgggca tcaggttacg ggtttcgagt ttcgggttcg
     5221 gaaccccaaa gcccataccc tcactcgaaa cccggcaccc gatacccaag ccgaaactac
     5281 gctttgcgtc gcgcgcgcgg atgatgcttg tcgtaagcac tacgcagatg acgatcggtg
     5341 atgtgcgtgt agatttgcgt cgtcgagatg tcgctgtgtc ccagcaacgc ctgcaccgaa
     5401 cgcgagtcag cgccgtgttg cagcaagtgc gttgcgaaac tgtgccggag agtgtgcggt
     5461 gaaatgtctt ttactccggc cttgcttgca tagcgcttga tcagcgccca ggcgaattgt
     5521 cgcgtgatag gcttaccgcg gttaaggaac aattgaggtg atgtcggttt cccgtaaccg
     5581 gcgcgaactg cgctgtactg ttgcagccag tggacagcac tcttaccaat cggcacgcgg
     5641 cgctccttgc tgcctttgcc gtgacagatc aacagaccgg attgcacgtc aacatccgcc
     5701 tgcttaagcg agatcaattc cgatactcgc aatcccgcag cgtacatcac ttcaattaat
     5761 gcgcgatcgc gcacgccttc ggcagtcgca atgtccggtg cagcaagtaa ttggttgatt
     5821 tcatcctccg ttagaaactg cgggaggtaa gcgaagcgtt gcggtgtatc gagatcttcc
     5881 gtcgggtgcc gtttgatgtg tccgtccagc atcaggaagc gaaacaagcc gcgggcggcg
     5941 ctgaccgcgc gtgccacgga ggacggtgcc agtccggcac gcgaaaggtt ggcaatccac
     6001 tttcgcaagt cagcgcgcgt caactgatca aggggcttcc cggctttgtc ggcccacagt
     6061 tgcagccgcg ccagatcgc
//
LOCUS       DASUEX010000414         2095 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_514110, whole genome shotgun sequence.
ACCESSION   DASUEX010000414 DASUEX010000000
VERSION     DASUEX010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_514110"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            317..1750
                     /locus_tag="VFV61_07010"
     CDS             317..1750
                     /locus_tag="VFV61_07010"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX4900266.1"
                     /translation="MSALSIQEPHNHSHAPNRFAVIPTVEKLAADPRFTGKGVTIAFL
                     DSGFYPHPDLTVPTNRIVAFHDVTGDQAALADEQRVESWHWHGTQTTVAAAGNGHLSG
                     GMYRGVASAAQLVLVKVSKQGRIADDSIARGLRWVIENRERFNIRVLNMSLGGDEDVP
                     CSQSEIDQLAEEAIRQGIVVVVAAGNSAERAPIPPANAPAVITVGGYDDGNRLSSRDL
                     AFYHSSFGTAADGTVKPELIAPAMWVAAPILPRTKDYLRAEALAEIVHAPDYQLPEIA
                     QNTREFEVQSKDPLAIRAAAERLINEHKIVATHYQHVDGTSFAAPIVASVIAQMLEAN
                     PALSPALVKNILISTADRVSGGSAIRQGYGVINARRAVDVAQREMHESDKHDFAAPRI
                     IDGKVVFSFHHHDAKDVALAGDFNNWNGAPFAKQANGFWNILIPVPAPGRYSYKFVVD
                     GAWLEDPNNVIKIEDGFGGLNSVLHIA"
BASE COUNT          452 a          560 c          607 g          476 t
ORIGIN      
        1 cgtcagtaaa tagccacggc cgtgaggccg tggctagtcg aaagataaaa tggttgagcg
       61 ccgaaggtgc gacataaatt ccattcacag cgcaaaagaa tgacggccct acgggcctcg
      121 accccatttc gggttttgat ccacggcctt acggccgcgg ctattgaatg ccgcgcctgc
      181 ggcgcttttg gaacccaaac cacgatgaca ttatttggtg agtcaaatat gtaattgccg
      241 ttcaaacggt ttccctgaat tgaaagctac ctcgggcacc gggcttaagc ctggtgtgaa
      301 tgaaactggg ctaactatga gcgcgctgtc cattcaagaa ccgcacaatc actcgcatgc
      361 gccaaatcgc tttgcggtga tccccactgt tgagaagctt gccgccgatc ccaggttcac
      421 cggtaagggg gtcacgattg cttttcttga ttcgggattc tatccgcatc ctgatctaac
      481 cgttccaacg aatcgcatcg ttgcttttca tgatgtcact ggtgaccagg cagcgctcgc
      541 tgacgagcaa cgcgttgagt cttggcattg gcatggaacg cagacgacgg ttgctgccgc
      601 cggtaatggt catctgtccg ggggtatgta tcgaggagtt gcttccgcgg cgcaactggt
      661 gctggtgaaa gtcagcaagc agggacgtat tgctgacgat agcatcgcgc gcggacttcg
      721 ctgggtgatc gagaatcgcg agcgcttcaa cattcgcgtg ctgaacatgt ctctcggtgg
      781 cgacgaagat gttccttgtt cgcagagtga gatcgatcaa cttgccgaag aagcaattcg
      841 ccagggcatt gtggtggtgg tcgccgccgg aaattctgcg gagcgagcgc cgatacctcc
      901 ggccaacgcg cctgccgtga ttactgtcgg cggttacgat gatggcaaca gactctcgtc
      961 gcgcgatctc gccttttatc actcgagttt cggaactgcc gcagatggca ccgtcaaacc
     1021 agaactgatc gccccggcga tgtgggttgc agctccaatt ctgccgcgta ccaaggatta
     1081 tctccgggcc gaagctcttg cggagattgt gcacgcaccg gactatcaac tgccggaaat
     1141 tgcgcaaaat acacgggaat tcgaagtgca gagcaaagac ccgctcgcga tccgggcggc
     1201 cgcagagcgt ttaatcaacg aacacaaaat tgtcgctact cattatcaac acgtggacgg
     1261 gacttccttc gcggcaccga tcgttgcatc cgtgattgcc cagatgctcg aagccaatcc
     1321 ggctctctcc ccggctttag taaagaacat tctgatttcg actgcggatc gtgtttccgg
     1381 tgggtccgcc atcaggcagg gctacggagt gatcaatgcg cgtcgcgctg tcgacgtggc
     1441 gcagcgggaa atgcatgaaa gcgacaagca tgatttcgcg gcaccgcgca taatcgacgg
     1501 caaggttgtt ttctcatttc accatcatga cgcgaaggat gtggcgctgg ccggcgattt
     1561 caacaattgg aacggcgctc cgttcgcaaa gcaagcgaac ggcttttgga acattcttat
     1621 cccagtgcct gcgccgggcc gctacagcta caagtttgtg gttgatggcg cgtggcttga
     1681 ggaccccaac aacgtaatca aaattgaaga tggcttcgga ggactgaact cggttctgca
     1741 tattgcatga acattcccgt ggttttgatt tcctgcgcaa tcgcgaccga gctgatttcc
     1801 cagtcacttg ataattcgct ttcgctgcgc aagcagttgc tattgcgaat tcacctggtt
     1861 tgttgtcggg tctgtgccag gttcaagcga cagcttgtga tgttacggcg gctggtgcgg
     1921 ctgagagcgc ggcttgaagg accggaggcg agtgagtgtt cgatcgcaaa tcgaggctct
     1981 tgaatggaat gcgaagtccg gaaggcgggc tcacgcccgg ctgcgccagt gcgatccaat
     2041 cgagactgga acgacgcgga gcgtcgcgca gattttagcc cggcgtttca acgcc
//
LOCUS       DASUEX010000415         2678 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_403960, whole genome shotgun sequence.
ACCESSION   DASUEX010000415 DASUEX010000000
VERSION     DASUEX010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403960"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1003)
                     /locus_tag="VFV61_07015"
     CDS             complement(<1..1003)
                     /locus_tag="VFV61_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900267.1"
                     /translation="MMRLRFRGFVVLFLLMVSVTAIFAQQRRPRTNPTPAAAPVQSDL
                     RIKYRSTTSGQSYESSTMIKGKRERSEMRMGYGSDITNITQCDLKRTIQISDAVRKYV
                     ITPMQTVDASSTTPRANSATPPAESGRRGGLVKYTTTINDTGERKEMFGFTARHIKSS
                     LLIESSTDACSPMNQRMETDGWYIDLTFGLDCDLGVPQMAGRPPLPQGGCRDEIVTKQ
                     LGSGRKGYPLIETTTMFGPDGTAMFTSTKEVIDLSREPLDAALFDVPAGYTETTNSQE
                     LYGAPSMASIMGDSGQPQTSQSLPSIEHPDSSMSAAMTKAPGMLRVGVVAINNQTDRS
                     "
     gene            complement(1064..>2678)
                     /locus_tag="VFV61_07020"
     CDS             complement(1064..>2678)
                     /locus_tag="VFV61_07020"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transferrin receptor-like dimerization
                     domain-containing protein"
                     /protein_id="HEX4900268.1"
                     /translation="RVAEQNGAVGLLIYSDPADDGYMQGDVYPKGPWRPATSAQRGSV
                     QYLFQYPGDPLTPGRPSIPGVERIKIEDATNLTSIPVQPISYGDAKQLLQPLRGPVRP
                     AGFQGGLPFSYHVGGTTDVRVHLKTEMDYQIRKIWNVVARIDGAEEKDRWVIMGNHRD
                     AWTFGAVDPNSGSSAMLEAARGFGQLLKNGWKPRRTIVLCSWDGEEYGLIGSTEWAEE
                     NAIELRQKAVAYLNMDAAVSGPNFGASSVPSLWKLIRGATRDVRDPKTGKSVYQQWQD
                     RANGERPEAEMTDAESGSDRAIAEARIGALGSGSDYTPFLQHLGVPSLDMGFGGDYGV
                     YHSAYDSFSWMSRFGDPTFVYHVAAAQLWGTVAMRLADAEGLPFDYVDYAKQIREFFN
                     ESFKTAKRLKLASGLNDKEMLEAIDDFAKEAARVEKNRQETIQEIQRTRVQANDSHPR
                     AVAKLQKINDVLMAVERNFIDERGLRGRPWYRHHIYAPGLYTGYAAQPLPDFRQGLDD
                     RNSANAKESLQRIIELLKGATETLRQARD"
BASE COUNT          554 a          795 c          698 g          631 t
ORIGIN      
        1 ctgatcgatc ggtttggtta ttgatcgcaa ccacaccaac acggagcatg ccaggagctt
       61 tcgtcatcgc tgctgacatg ctgctgtcgg ggtgctcgat gctcggcagc gattgacttg
      121 tttgcggctg gcctgagtcg cccatgatcg acgccatcga cggcgcccca taaagctcct
      181 gagagtttgt cgtctcggta taacccgcgg gtacgtcgaa caaagccgca tccaaaggct
      241 cgcgcgagag gtcaatcacc tctttcgtcg aggtaaacat cgcggtgcca tcgggaccga
      301 acatcgtcgt ggtctcgata agcggataac ccttgcgccc tgaacctaac tgcttcgtga
      361 ctatctcgtc tcgacatcca ccttgtggaa gcggtggcct gccggccatc tgcggaacgc
      421 ccagatcgca atcgagacca aatgtcagat cgatatacca accgtctgtc tccattcgtt
      481 ggttcattgg actgcacgca tctgtcgacg actcgattaa tagcgacgac ttgatgtgac
      541 gcgccgtgaa gccaaacatt tcttttcgtt cgccggtgtc gttaatcgtc gtggtgtact
      601 tgactagtcc accacgccgt cccgactcgg cgggaggcgt tgctgagtta gcgcgcggag
      661 tcgtgctcga cgcgtcgacg gtttgcatcg gcgtgatcac gtatttgcgc accgcatcgc
      721 tgatttgaat cgtgcgcttt agatcgcact gcgtgatgtt ggtgatgtct gaaccataac
      781 ccatgcgcat ctcgctacgt tcgcgcttgc ccttgatcat cgtcgagctc tcgtacgact
      841 gacctgaggt agtgctgcgg tacttaatac gtaaatccga ttgcacgggc gccgcggccg
      901 gagtgggatt cgtccgcggg cgtcgctgct gggcgaagat cgcagttacc gaaaccatta
      961 aaaggaacaa gactacaaaa ccccgaaacc ttaatctcat cattgcttct ccttaagttg
     1021 ttcttactga cccgcctgcc ggcggtgatt tctttgcaaa ctactagtcc cgcgcctgtc
     1081 gcaacgtctc cgttgcccct ttcaacaatt caatgatccg ctgaagagat tctttggcgt
     1141 tggcactatt gcgatcatct agtccctggc gaaaatccgg cagcggttgc gccgcatatc
     1201 cggtgtagag gccaggggcg taaatgtgat gtcgatacca gggccggcca cgcagaccac
     1261 gctcgtcgat aaagtttcgt tcgactgcca tcagtacgtc gttgatcttt tgcagttttg
     1321 cgaccgctcg cggatgactg tcgttagcct gaacacgagt ccgctggatc tcctggatag
     1381 tctcttgccg attcttctct acgcgcgctg cttcctttgc aaaatcatcg atcgcttcga
     1441 gcatttcctt gtcgttcaaa cctgacgcaa gtttcaagcg cttcgccgtt ttgaaactct
     1501 cattgaagaa ctcgcgaatt tgtttggcat agtccacata gtcgaaaggc aacccctccg
     1561 cgtccgcgag tcgcatagcc accgtgcccc agagctgcgc ggccgctaca tgatatacga
     1621 aggtgggatc accaaagcgc gacatccagc taaaggagtc ataagcggag tggtaaacac
     1681 cgtaatcgcc accaaaaccc atgtcgagcg aaggcacgcc gagatgttgt agaaacggtg
     1741 tgtaatcaga gcccgacccc aacgcaccaa tgcgcgcttc ggcaatcgca cgatcagagc
     1801 ctgactcggc atctgtcatc tcagcttccg gccgctcccc gttagcacga tcctgccatt
     1861 gctgataaac gcttttgcca gtctttggat cacgcacatc gcgcgttgcc ccgcggatca
     1921 atttccacaa cgacggcacg gaggaagctc cgaagtttgg gcccgacacg gctgcatcca
     1981 tgtttagata cgccaccgct ttttgtctta actcgatggc gttttcctcc gcccattcag
     2041 tcgaaccgat caggccatac tcttcgccat cccaactaca aagcacgata gttcgcctgg
     2101 gcttccagcc attcttaagg agctggccaa aaccacgcgc agcttcgagc atggcagacg
     2161 agccgctgtt aggatcgact gcgccgaatg tccacgcgtc gcgatggttt cccatgatca
     2221 cccaacggtc cttctcttca gcgccatcga tgcgcgcgac tacgttccag atcttccgaa
     2281 tctggtaatc catctcagtc ttcaaatgta cgcggacgtc cgttgtgcca ccgacgtggt
     2341 acgaaaaagg cagcccgcct tgaaaacccg ccggtcgcac tggtccgcgc agtggctgca
     2401 acagttgttt ggcgtcgcca tacgatatcg gctgcacggg aatgctcgtg aggtttgtcg
     2461 cgtcttcgat tttgattcgt tccactccgg ggattgaagg cctacccggt gttaggggat
     2521 cgcccggata ttgaaacagg tactgcactg agcctctttg ggcagacgtc gcaggacgcc
     2581 agggcccttt aggatagaca tcaccctgca tgtagccatc gtcggcagga tcggaataga
     2641 tgagcaaacc cacggcgccg ttctgttccg cgaccctg
//
LOCUS       DASUEX010000416         4677 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_751500, whole genome shotgun sequence.
ACCESSION   DASUEX010000416 DASUEX010000000
VERSION     DASUEX010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4677
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_751500"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1494
                     /locus_tag="VFV61_07025"
     CDS             <1..1494
                     /locus_tag="VFV61_07025"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HEX4900269.1"
                     /translation="TGPNGSVFIVSLSNGAVYEIKSKPSLIFTASLNGAQQVPPVNTP
                     ATGTATLILSPDEQSARVSLNFSGLTSSETDAHIHGPAPAGMNAGVLFPLPLGQLGDF
                     KIDLTSQQVADLKAGLHYINVHSSNFMSGEIRGQFGPLGTASVVVSAASSFAVNEGQG
                     GVPITFTRLGNTGNAASVSYATSDTAGLANCNVINGIASSRCDYAASVGTINFAAGEA
                     SKTISIPIVDDAYAEGPETFTITLSNPSGLVLGPPTTAAITINDNETVNGPNPIDQTA
                     FFVRQHYIDFLGREPDPAGFQAWQNTINNCPAGDITCDRIHVSGAFFQSPEFQQRGYF
                     VYRFYPVGFGRKPDYAEFIPDLARVSGFLSDAQLEAAKVAFVDDFMNRQAFMATYNGL
                     NNTQYVDTLLATAGVTSPNRDFWIAALGNGTRTRATVLRDIAESPEVYNKYYNQAFVV
                     MQYFGYLRRDPDSLYLNWIQELDTTGNARNMINGFVNSLEYRFRFGP"
     gene            complement(1540..1899)
                     /locus_tag="VFV61_07030"
     CDS             complement(1540..1899)
                     /locus_tag="VFV61_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900270.1"
                     /translation="MTQSFAPGDDLLFQLESGFGLLRILAIEGSGRETVWHLMAYEEF
                     FPSAEAAEAALAEPQRLSVRQSHLALTDRAFERTPAARLGNRPLTDDELHPYREWLES
                     EKRLVSDRSALLMLGIR"
     gene            1995..2552
                     /locus_tag="VFV61_07035"
     CDS             1995..2552
                     /locus_tag="VFV61_07035"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEX4900271.1"
                     /translation="MSQFDLSKDYYFILGAETETTGQDLERLYKRRAAQMHPDRGGSE
                     EEMKSLNEAYRVLKDETTRHEYDQQRQKPLASRGAIVSAPAAKDVGLFGHFLSAFLCL
                     LVGLFLLFLVRVQWIWFLWPLVILALFVIVFGVLMAHSAMLAANASLPLSNPVKRHRP
                     VQEAMFWTAVAVAGYGLYLLLTSVG"
     gene            2560..2931
                     /locus_tag="VFV61_07040"
     CDS             2560..2931
                     /locus_tag="VFV61_07040"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX4900272.1"
                     /translation="MEIKIKGMDLLFLEVNNLEESLAFYHGQLGLAIESHNPSSEPPM
                     ATLRAGDLKITLAQNLETMLRRGRGAHFFLGVADVDEYYDYLRTKDVEVKPPVEEGWG
                     GRFITVQDPDKYRFFFVTWNV"
     gene            3145..4161
                     /locus_tag="VFV61_07045"
     CDS             3145..4161
                     /locus_tag="VFV61_07045"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX4900273.1"
                     /translation="MQYVANPPNPWLTHSVEWLGEPPETKLEVFEETDTRTIISHNNS
                     PDVSFDYSLNCYRGCIHGCTYCFSRPTHEYLGYGAGTDFDRKIVVKVNAPVLLRKELM
                     RPSWRGDEIVFSFTSDPYIPLEAHYRLTRSCLQVCAEFRNPVGVITKSALIRRDIDVL
                     QQLTRDARLGVFFTIPFTDRESARAVEPYAPLPEARFHAMKELASAGIKVGIGIAPVI
                     PGLTTDIPELLKRAKDAGATHAFINMLRLPGSVAPYFQERLHERLPTKANRILNRIRD
                     AREGKINSSVFGERMRGKGQYWEATARLFKIHCERLGFNKKTPSSIESNTTFARPTLQ
                     PSLF"
     gene            4271..>4677
                     /locus_tag="VFV61_07050"
     CDS             4271..>4677
                     /locus_tag="VFV61_07050"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX4900274.1"
                     /translation="MMLIRDVIRNREPYSMKSSATVQDAAEFMASRNIGAVCVVDDGG
                     KLLGVFSERDVVNRVVLQKRDPTSLRVGDVTSELRAVIRCDETPHQALERMELIGTRH
                     LPVVDGERWVGMLSMRDLMRVELSEQGDEIKLL"
BASE COUNT         1064 a         1221 c         1270 g         1122 t
ORIGIN      
        1 acgggaccga atggtagcgt tttcatagtt tcgctctcga acggagccgt ctacgaaatt
       61 aagtctaagc catcgctcat ctttacggcc tcgctaaacg gcgcgcaaca agtaccccct
      121 gtaaatacgc ccgcaactgg aactgcgacg ctaattctaa gtcctgacga acagagtgct
      181 cgcgtctcgc tgaacttcag cgggcttacg agctcagaga ccgacgcaca cattcacgga
      241 ccagcaccag caggcatgaa cgccggcgtc ttgttcccgc tgccattggg tcaattgggc
      301 gattttaaaa tcgatttgac gtcgcaacag gttgctgatc taaaggccgg cttgcactac
      361 atcaacgttc attcgagcaa ttttatgagc ggcgaaattc gcgggcagtt tggtccctta
      421 gggacggcca gcgtggttgt ctcggcggct tcaagttttg cggtgaatga aggccagggc
      481 ggagtgccga tcacctttac gcggctcggg aataccggaa acgctgcttc ggttagttac
      541 gccacgagcg acacggctgg tttggccaat tgtaacgtga tcaatggcat cgcctcttcg
      601 cgttgcgact acgctgcctc ggtagggaca atcaatttcg ctgccggtga agcttcaaag
      661 acaatctcga ttccaatcgt ggacgatgcc tacgccgaag ggccggaaac attcaccatc
      721 actttgtcga accccagcgg gctcgttctc gggccgccaa ccaccgcagc gattactatc
      781 aatgacaacg agacggtaaa cggtcctaat ccaatcgatc aaactgcgtt ctttgtgcgc
      841 cagcactaca tcgacttctt agggcgcgaa cctgatcccg ctggtttcca ggcgtggcag
      901 aacactatca acaactgtcc cgccggagac atcacttgcg atcggattca tgtgtctgga
      961 gcgttcttcc agtcgcccga atttcagcaa cgtggttact tcgtctatcg cttctacccg
     1021 gtggggtttg gacgcaagcc tgactacgcc gagtttattc cggatctcgc gcgtgtcagc
     1081 ggcttcttaa gtgatgctca gttggaggcg gcgaaagtcg cgtttgtaga tgatttcatg
     1141 aatcggcaag cgtttatggc gacctataac ggactgaaca acactcagta tgtcgacacc
     1201 ttactggcga cggctggggt cacttcgccg aaccgagatt tttggattgc agcgttagga
     1261 aatggaacgc gaacgcgagc gacagtgctg cgagatattg ccgagagtcc tgaggtttac
     1321 aacaagtatt acaaccaggc gtttgtggta atgcagtact tcggttactt gcggcgagat
     1381 cctgactcac tctacctgaa ttggattcag gaattagata ccactggaaa cgctcgcaac
     1441 atgatcaatg gctttgttaa ctctctcgag tatcgttttc gctttggacc ctagatattg
     1501 gcgattgccg attgccgact gccaattgcc aatcggcaat tatcgtattc caagcattag
     1561 aagggccgag cggtcagaaa caagtctctt ttctgattcc agccattccc gataaggatg
     1621 cagttcatcg tccgttaagg gtcgattgcc gagacgagcg gccggcgtgc gttcgaacgc
     1681 ccggtcggtt agtgccaaat gagactgtcg cacactgagt ctctgaggtt cagcgagtgc
     1741 tgcttcggca gcttccgccg agggaaagaa ttcttcataa gccatcagat gccaaactgt
     1801 ttcccgaccg cttccttcaa tcgccaggat ccgaagcaac ccgaaaccgc tttccagttg
     1861 aaacaacagg tcatcaccag gcgcaaaaga ctgagtcatg aatccagtct aacatgcgta
     1921 tcttgctcgc cgggagactt ccgcaaacgc aaattgcaaa accaaaaatg taaacttaga
     1981 atcggcagtc agtcatgagc caattcgatc tcagcaagga ttattacttt atcctgggcg
     2041 ctgaaacaga aacgactggc caggatctgg agcgactcta caagcgccgc gccgcgcaaa
     2101 tgcatcccga ccgtggcggc agcgaagagg aaatgaaatc gctcaacgag gcttatcgtg
     2161 ttctcaaaga tgaaacgaca cgacacgaat acgaccagca gcgccaaaag ccgctggcct
     2221 ccaggggcgc aatcgtttca gcgcccgcag caaaagatgt cggactcttc ggtcacttct
     2281 tgagtgcttt tctctgcctg ctcgtaggtt tatttcttct ctttctagtg cgtgtgcaat
     2341 ggatctggtt tttgtggccg ttggtgattc tggcgctctt cgtaatcgtg tttggtgtgc
     2401 tgatggctca cagcgccatg ctggcagcga acgcgtcgtt gcccttgagc aatccagtga
     2461 agcgacatcg gccagtgcag gaggcgatgt tctggaccgc cgtcgcagtg gccggctatg
     2521 gtttgtacct gcttttaacc agcgtgggtt agcgttcaag tggaaatcaa gatcaaggga
     2581 atggacttgt tgtttctcga ggttaacaac cttgaggagt cgctggcctt ctatcacggc
     2641 cagctgggtt tggcgatcga gtcgcacaat ccgagcagcg aaccgccaat ggcaacgttg
     2701 cgtgcgggtg atttgaagat cactctcgcc cagaatctgg aaacgatgct gcgtcgagga
     2761 cgcggcgcgc atttcttcct cggcgttgcc gatgttgatg agtactacga ttaccttcgc
     2821 actaaggatg tcgaagtgaa gcctccggtt gaggaagggt ggggcggccg ctttatcacg
     2881 gtgcaggatc ccgataagta tcgtttcttc ttcgtcacct ggaacgtttg attgccgatt
     2941 gtccgattgc cgattgatga ttggtctgtc aatcacggct tgccgcctca aattacggag
     3001 ttcaaagcag aacacaattg gtatttagcg gcctagacaa tcagcattcc gaaatcagca
     3061 aatccgcgat tcacgacatt cggcacttgg caatcggcaa ttgccaatcg tatactgttg
     3121 catagatagg acagacttgc atctatgcag tacgtggcga atcctcccaa tccctggctc
     3181 acgcattcgg tcgaatggct gggcgaacca ccggaaacga agcttgaggt ctttgaagag
     3241 acggacacgc gcaccatcat cagtcacaac aacagccctg acgtcagttt cgactactca
     3301 ttgaactgct atcgcggctg cattcacggt tgtacctatt gtttcagccg gccgactcac
     3361 gagtatctcg gctacggtgc cggcacagac ttcgatcgca agatcgtggt taaggtgaac
     3421 gcacccgtgc tgttgcgtaa ggagctgatg aggccttctt ggagaggcga cgagattgtg
     3481 ttttctttta cttcagatcc gtatattccg ctcgaagctc actaccggtt aacgcgaagt
     3541 tgtttgcagg tgtgcgcgga gtttcgcaat ccagtcggtg tgattactaa gtcggccttg
     3601 atacgccgtg acatagacgt cttgcagcag ctaacccgcg atgcgcggct cggagtcttc
     3661 tttacgattc cgtttacgga tcgtgaatct gcacgtgcgg tggaacctta cgcgccgctt
     3721 cccgaggcgc gctttcatgc aatgaaggag ctggccagtg ccggtattaa agtcggaata
     3781 ggcattgcac ccgtgatccc aggtttgacg accgacattc cggaactact caaacgagca
     3841 aaagatgctg gggcaacaca tgccttcatc aacatgctgc gtctgcccgg cagtgtcgct
     3901 ccttattttc aggaacgttt gcacgagcgc ttacccacga aggcaaaccg catcttgaat
     3961 cgaattagag atgctcgcga agggaagatt aactccagcg tttttggtga gcgcatgcgg
     4021 gggaagggcc agtattggga agcaacggcg cgcctcttca agattcactg cgaacggctt
     4081 ggtttcaata agaaaactcc tagtagtatc gaatcaaaca caactttcgc cagaccaacc
     4141 ttgcagccgt cgttgttctg acttctgact cctgcccccg actcctgcgt ctgtttttcc
     4201 ctagagacag aacccgcttg ctcgcctata atgatcgacg tatccgaaca accgaatcag
     4261 gagctgcgcc atgatgctga tcagagatgt gattcgcaac cgtgagcctt actcgatgaa
     4321 gtcgtctgcg actgtccagg acgctgcgga gtttatggcg tcacgaaata ttggcgcggt
     4381 ttgcgtagta gatgacggcg ggaagttgct cggtgttttt tcggaacggg atgttgtcaa
     4441 tcgcgtcgtc ttgcagaagc gcgacccgac ttcgttgaga gtgggcgatg taaccagcga
     4501 gttgcgcgct gtgatcaggt gcgatgagac tccgcatcag gccctggagc ggatggaact
     4561 gatcggcacg cggcatctgc cggtagtcga tggcgagcgt tgggtcggta tgctttcgat
     4621 gcgagatctg atgcgtgtag agttgagcga gcaaggcgac gagatcaagt tgctgca
//
LOCUS       DASUEX010000417         5333 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1321912, whole genome shotgun sequence.
ACCESSION   DASUEX010000417 DASUEX010000000
VERSION     DASUEX010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5333
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1321912"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="VFV61_07055"
     CDS             <1..364
                     /locus_tag="VFV61_07055"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX4900275.1"
                     /translation="NLNVLVVEDDDDSRYVMRLELEQLGYLVSEAENGVKAVEIALRE
                     HPDIILMDLALPEMDGLEATARIRANAEMSDVPIIAVTAHQESDWREEAKASGFNAYV
                     TKPIDGGWLSELIPGLLV"
     gene            399..1226
                     /locus_tag="VFV61_07060"
     CDS             399..1226
                     /locus_tag="VFV61_07060"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX4900276.1"
                     /translation="MVSTPDAIQIRPLRTIAELKAVEELQVEVWGCSEREVLPSLTLI
                     PLLDIGGVLLGAFDGEELVGFVIGFPGFDGGQAILHSDMLAVRSIHRSTGLGYRLKLE
                     QRSVALANGIEKITWTFDPLQAANAHLNFRKLGVIADCYKINYYGETSSALHRTGTDR
                     LWVTWLLRSDHVTRRISVSGDKSSVNAEERPALISVGPDGEPVSAVLDVRQNSWRIEI
                     PNDINAMVDFDPARANRWREATRAQFTAAFAANFAATDFFKAPTSTRAGFYILTRTN"
     gene            complement(1219..2271)
                     /gene="asd"
                     /locus_tag="VFV61_07065"
     CDS             complement(1219..2271)
                     /gene="asd"
                     /locus_tag="VFV61_07065"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF006416.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEX4900277.1"
                     /translation="MTRKFRVGILGATGTVGQRFIQLLEHHPQFEITALAASDRSQGK
                     PYAEACSWRLAGELPQSLTQLVVQAPKPPLDCELVFSSLPGDIARETEGNFAKAGYPV
                     ISNSSAYRMDPDVPLLIPEVNHEHLSLLAAQRVRFGGRGFIVTNPNCSTIMLALALAP
                     LHQFFGIQAVVATTMQALSGAGYPGVAALDILDNVLPYIGGEEEKIEQETGKILGRAR
                     DNYIEHATMKVSAQCNRVNVADGHMAAVRVKLDLDSNLDEVCATFSAFSSLPQELQLH
                     SAPQWPIILRDERDRPQPKLDRDAGAGMSITIGRVQTDSVLDYRFVVLSHNTIRGAAG
                     AAILNAELLIAKGILS"
     gene            complement(2268..3422)
                     /locus_tag="VFV61_07070"
     CDS             complement(2268..3422)
                     /locus_tag="VFV61_07070"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX4900278.1"
                     /translation="MTDFEPPLFLRNIEKSPIRQISDRAKPGDISLGLGEPDLPTPDV
                     IRNAAIKVIAEEQNGYTLQAGLPRLRERVALDYPQLKLTPDQVVITAGSQEALYLALL
                     TLINAGDEVLIPDPGFVAYPTIIRMAGGIPVTYRMPAAGHFSFDATAFRQALTKRTKV
                     VVCVSPSNPTGRTLSADDLSHISAALKDTGIYLLSDEIYRDIYFGNERPPSAASFYER
                     TIVISGLSKSMSMTGWRLGWICGDAAVIRAALVLHGYVTTCASTVSQKAALAAWTDEG
                     AAARASIRQTFHQRRDYLVDLIKSELGLNAVTPDGAFYTMVDVRNYGSSVAVAEKLLE
                     QGVVTVPGSAFGSEGEGFLRISFCADNSKLAEGVARMKKALAAPLGVASP"
     gene            complement(3419..4252)
                     /locus_tag="VFV61_07075"
     CDS             complement(3419..4252)
                     /locus_tag="VFV61_07075"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: protein motif:HMM:NF008808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HEX4900279.1"
                     /translation="MPLREQIEALAAKKDSPFTDDDRELFAAFKAALNGGEIRAAEKT
                     ASGQWLVNSWVKQGILLGFRMGSLVDMSAAGPLRFLDKDTYPVRALTIADEVRIVPGG
                     SSIRDGAFIARGVVCMPPMYVNAGAYVDEGTMIDSHALVGSCAQIGKRVHLSAAAQVG
                     GVLEPIGAVPVIIEDDVLVGGGCGVYEGTIVRERAVLAAGTILTGSTPVYDLVRETVY
                     QRKPDAPLEIPVGAVVVPGSRAVQSERGREWGLSIYAAVIVKYRDAKTDRAVQLEDYL
                     R"
     gene            complement(4239..5162)
                     /gene="dapA"
                     /locus_tag="VFV61_07080"
     CDS             complement(4239..5162)
                     /gene="dapA"
                     /locus_tag="VFV61_07080"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEX4900280.1"
                     /translation="MTTNIGWLRGCATALVTPFTRDGAIDEVALRRLVEYQIAGGVKL
                     LVPCGTTGESATMTEAEDQQVIKLVIETAKGRARVIAGTGSNATAAAIEYSQTARALG
                     ADAVLVVAPFYNKPTQDGLYAHFRAVAESVGDLPVAIYNVPGRTSSNITAQTTLRLAR
                     DVPNIVAVKEASGNLSQIMEILRERPAGFRVLSGDDSFTFPLLALGAEGLISVAANEA
                     PALMSQMINLALEGNWQDARELHYRLLPLMEVNFIESSPGPVKAALAMMGLIEETYRL
                     PLVPISEQSKTKLHPVLAELGLLKETSHATA"
BASE COUNT         1198 a         1515 c         1480 g         1140 t
ORIGIN      
        1 aaatctaaac gtcctggtgg ttgaggatga cgacgactcg cgctacgtca tgagattaga
       61 gctggagcag ctcggctatc tcgtgagcga agcggagaac ggcgtcaaag ccgtggagat
      121 tgctttgcgc gaacacccgg acatcatcct gatggatcta gcgctgccgg aaatggatgg
      181 tctggaagcc acggcacgaa ttcgcgccaa tgcggaaatg agtgacgtac cgattattgc
      241 tgttaccgct catcaggaaa gtgactggcg cgaagaagca aaggcttccg gcttcaatgc
      301 ctacgtcacg aaacctattg atggcggctg gctaagcgaa ctgatcccgg ggcttctggt
      361 ttagaactct ttcgccttcc gatactccgc tctagcagat ggttagcaca cctgacgcga
      421 ttcaaattcg ccctctgaga acaattgcag agctcaaagc cgtcgaagag ttgcaagtgg
      481 aggtctgggg ttgcagcgag cgcgaggtgc tgcccagtct cacactcatt ccgctgctcg
      541 atattggtgg agtcttgttg ggagctttcg atggcgagga gttagtcgga ttcgtcatcg
      601 gtttccccgg atttgacggg gggcaagcta ttctgcattc ggatatgctc gccgtgagat
      661 cgattcatcg ctccacaggt ctgggttaca ggctgaaact cgaacagcga agcgttgctc
      721 tggcaaacgg aatcgaaaaa atcacctgga cttttgatcc gctgcaggcg gcaaacgccc
      781 atttgaattt ccgtaaactg ggcgtcattg cggactgcta caagatcaac tactacggtg
      841 aaacctcgag cgcccttcat cgcaccggaa cggatcggtt gtgggtgacg tggttgttgc
      901 gcagcgatca tgttacgcgg cgaatttcgg tctcgggcga taaatcgtca gtgaatgcgg
      961 aagagagacc ggcgctgata tcagtcggtc cagatggcga gcccgtctcg gcggttctcg
     1021 atgtgagaca aaactcatgg agaatcgaaa tacctaacga catcaacgcg atggtggatt
     1081 tcgatccagc gcgtgcgaat cgatggcgcg aagctacgcg cgctcagttc acagcagcat
     1141 tcgcagcgaa tttcgcggcg acagattttt tcaaagcccc gacgagtacg cgagctggtt
     1201 tctatatact gactcgcact aactgagtat ccccttagca atcagcaact ctgcgttcag
     1261 gatcgcggca cccgcggcgc cgcgaatggt gttatgactc aacaccacga agcggtagtc
     1321 aagcacgctg tcggtctgca cgcgaccaat agtaatactc atcccagccc cggcatctcg
     1381 gtccagtttc ggctgagggc gatcccgctc atcgcgtagt atgattggcc actgcggcgc
     1441 ggagtggagt tgtagctcct gtggcaagga actgaatgca ctgaacgtcg cgcatacttc
     1501 atcaagattt gaatcaagat ccagtttcac tcgcacagca gccatgtgcc cgtcggcgac
     1561 gttcacacga ttacactgag cgctcacttt catggtcgcg tgttcgatat aattatcgcg
     1621 cgcgcggcca agtatcttcc ctgtctcctg ttcaatcttt tcttcctcgc ctccaatata
     1681 cggcaacaca ttatccagaa tatcgagcgc ggcaacgccg ggatatccgg caccagacaa
     1741 agcctgcatc gtcgtcgcga caactgcttg gataccaaaa aactggtgca gcggcgccag
     1801 tgccaatgcc agcatgattg ttgaacaatt cggatttgtg acgataaaac cgcggccgcc
     1861 aaatcttact cgctgtgcgg cgagcagcga caggtgttcg tgatttactt ccgggataag
     1921 taagggaacg tcgggatcca ttcgatacgc cgatgagttg ctgattaccg gatatccggc
     1981 tttagcgaag ttaccttccg tttccctggc gatgtcgccc ggcagactcg agaagactaa
     2041 ctcgcaatcg agagggggct tcggtgcctg aactaccaac tgcgtcagtg actggggcaa
     2101 ctcgccagct aagcgccacg aacacgcttc cgcgtacggc ttaccctgag agcgatccga
     2161 tgctgcgagc gcagtgattt caaactgcgg gtgatgttcc aacagttgga tgaagcgctg
     2221 gccgacggtg ccggtagcgc caagaattcc cacgcggaat ttcctggtca tggggaagcc
     2281 actccgagcg gggccgcaag tgctttcttc atgcgcgcca caccttctgc gagtttggaa
     2341 ttatcagcgc aaaacgagat gcgtagaaaa ccctcacctt cactgccgaa tgcagacccg
     2401 ggaaccgtga ccaccccctg ctcgagtaac ttttcggcaa ccgcgacgga cgatccgtag
     2461 ttgcggacat ccaccatggt gtagaacgcg ccatcaggag ttactgcatt caagccaagc
     2521 tcgcttttga tcaagtcaac cagatagtct cgacgctggt gaaacgtctg tcggatcgag
     2581 gcccgggcag cggcgccttc gtcagtccag gcagccagcg cagctttttg cgagactgta
     2641 gaagcgcagg ttgtcacgta accgtgcaag acaagcgcag cgcgaatcac tgcggcgtcg
     2701 ccacaaatcc agcctaaacg ccagcccgtc atgctcattg attttgagag accgctgata
     2761 acaatcgttc gctcatagaa agaagctgct gatggcggac gctcgttccc gaaatagatg
     2821 tcccggtaaa tctcatcgct gagtagatat atgccagtgt ccttcagtgc tgcgctaatg
     2881 tgagagagat cgtctgcgga caatgtgcgg ccggttgggt tggaaggact gacgcaaacg
     2941 acaactttgg tccgcttggt tagcgcctgc cggaaagcgg tagcgtcgaa tgaaaagtgg
     3001 ccggcggctg gcatgcgata ggtgaccggg atgccgccgg ccattcgtat gattgtcggg
     3061 taagcaacaa agcccggatc gggaatcaga acctcgtcgc cggcattaat caaagtcagc
     3121 aaagccaggt agagcgcctc ttgcgaaccg gcggtgatga caacctggtc cggcgtcaac
     3181 tttaactggg ggtagtccag ggccacgcgt tcacggaggc ggggaagccc cgcttgaagc
     3241 gtgtagccat tttgctcttc cgcgatcacc ttgatcgcgg cgttgcgaat cacatctggt
     3301 gtaggcaggt ccggttcgcc tagtcccagg ctgatatcgc caggtttggc ccggtctgaa
     3361 atctgacgaa tagggctctt ctcaatgttc cgcagaaaca gcggcggctc gaagtcggtc
     3421 aacgcagata gtcctccaat tgcactgccc gatcagtctt ggcatcgcga tacttcacaa
     3481 ttactgccgc gtagatcgac agtccccact cacgaccgcg ctcactctgc acggcccgcg
     3541 agccgggtac caccaccgcg ccgacgggaa tttccagggg tgcatccggc tttcgttgat
     3601 agacagtttc ccgcactaga tcgtagacag gtgtggagcc ggtcaggatc gttcccgcag
     3661 ccaacaccgc gcgctcgcgc acgatagtgc cttcgtaaac gccacagccg ccgcccacca
     3721 gcacgtcatc ttcgataatt accggcaccg cgcctatcgg ttccaacacc ccacccacct
     3781 gggcagcagc ggaaagatgc actcgcttac cgatctgcgc gcaagagccg acgagcgcgt
     3841 ggctgtcgat cattgtgcct tcgtcaacat aggctccggc attaacgtac atcggtggca
     3901 tgcagaccac gccccgcgcg atgaaggctc cgtcgcgaat actggaaccg ccaggcacga
     3961 ttcgcacctc atctgcgata gtcaacgcgc gcactggata ggtgtcctta tccaggaatc
     4021 gcaggggacc agcagcggac atatcaacca acgaacccat gcgaaaacca aggagaattc
     4081 cctgcttcac ccacgagttg actaaccatt gcccggaagc ggtcttctcg gcggcgcgaa
     4141 tctcgccgcc gttgagtgca gctttgaatg cggcgaagag ctcacgatcg tcatccgtga
     4201 aaggagagtc ttttttggca gcgagagctt cgatttgttc acgcagtggc atgcgaagtt
     4261 tcctttagca gtcccaactc cgcgagcacc gggtgcagtt ttgtcttgct ctgctcactt
     4321 atgggcacaa gtggcaagcg ataggtctct tcaatcaaac ccatcatcgc gagggccgct
     4381 ttcacgggac cgggactcga ctcgataaag ttcacctcca taagcggcag cagtcgatag
     4441 tgcaactcac gcgcgtcctg ccagttccct tccagagcga gattaatcat ttgcgacatc
     4501 agcgccggtg cttcattagc ggctaccgag atcaagcctt cagcgcctag cgcgaggagg
     4561 ggaaaagtaa atgaatcgtc gccggagagc acacgaaatc cagcgggccg ctcacgtagt
     4621 atctccatga tctgcgaaag gttccctgat gcttctttga ccgcgacaat gtttggcacg
     4681 tcgcgagcca gccgcagtgt tgtttgcgcg gtgatgtttg aagaagtacg accgggaacg
     4741 ttgtagatcg ccaccggcag atcgccgacg gactctgcca ccgcacgaaa gtgcgcatac
     4801 aagccgtctt gagtgggttt gttgtagaaa ggagcaacca ccagaacggc atcagcgcct
     4861 agcgcgcgcg cggtttgcga atactcaatc gccgccgccg tcgcgttgct tccggtgccc
     4921 gcgatcacac gtgcacgacc cttggccgtc tcgatcacaa gtttgatgac ctgctgatcc
     4981 tcggcttccg tcatcgtcgc actttcccca gtggtgccac agggcacgag taacttcacg
     5041 cctccagcaa tctgatactc aacgaggcgc cgcaaagcga cttcatcaat tgcgccgtcg
     5101 cgagtgaatg gagttaccag cgcagtggcg caaccgcgca gccaaccaat gttagtggtc
     5161 atgagtcctc cttatttgga ttgccgattg gcgattgcca attgccaatt gctttgttgg
     5221 ctcgggcttt tggtattcgt caaattggta cttaacaatc ggcactcggc aattgggaat
     5281 cggcaatcac ctcgttgatc atgtccgaaa actcaaacac gcccttgcga ccg
//
LOCUS       DASUEX010000418         3851 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_404964, whole genome shotgun sequence.
ACCESSION   DASUEX010000418 DASUEX010000000
VERSION     DASUEX010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3851
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404964"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="VFV61_07085"
     CDS             <1..262
                     /locus_tag="VFV61_07085"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEX4900281.1"
                     /translation="FPEGANSVGCHQLIGDVWEWTTSDYVPYPGFKSEFDEYNDKWFV
                     NQKVLRGGSFATPQRHIRSTYRNFFHAHERWMVSGFRCAKDL"
     gene            281..1339
                     /locus_tag="VFV61_07090"
     CDS             281..1339
                     /locus_tag="VFV61_07090"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX4900282.1"
                     /translation="MPTPPDTESRTAVKEFLLAEYHNVADSFWKNEQTGETRVNWFIG
                     IVTAAVGGLFGLTTAEKRPHGEPLRLIYLAALFALLSFGVVTLLRMMKRNETTDGYKK
                     DGKRIRRLFAASFDDAGILRNYGSFGDGSQPKETLRKLGGLSHTVSTINSLLLAGIAA
                     AAVYPFGISDESKRVAQILLTYRISPLVFILAFVGQFFWIRRAEEKSRQRVRTTTHAG
                     GIVFSDEGGKVNYLLVRPSKKKPDKDEWLFPKGHIKEGEEQWESAVREVREEAGVVGH
                     LICPVGIDAYEVEEKPLQVKFYLLRSEAIVPRTEDREITWVAFTAALKNLSYLGSKQL
                     LVKAELKRRLAQKEYRRS"
     gene            1336..1998
                     /locus_tag="VFV61_07095"
     CDS             1336..1998
                     /locus_tag="VFV61_07095"
                     /inference="COORDINATES: protein motif:HMM:NF016240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HEX4900283.1"
                     /translation="MTTILSDIEVRVLGALVEKQVTTPEYYPLTLNSLTLACNQKNNR
                     NPVTGYSENEVAQALETLREKNLTYVFHGSTSRVPKYKHVMPEVLHLTPPETALLCVL
                     MLRGPQTIGELRGHGARLYTFTSLDEVDATVNALITHDPDPMVVRLARQPGQKEARFS
                     HLLAGVPAEDLASDTPAPRPSSSRHGDRERIEQLEQTVTALREEIQALKQQFGEFKKQ
                     FE"
     gene            2091..2708
                     /locus_tag="VFV61_07100"
     CDS             2091..2708
                     /locus_tag="VFV61_07100"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX4900284.1"
                     /translation="MKSTFKSDHPVTNEAAKTATGKTLDQWFAELDKENGLKLGRREI
                     NNRLYAQKLDPWWCVTIAVEYEKHHDVRKKDGLFEGYFVCATKTIGSPPAAVFKAWSS
                     GAELSKWFGPATKAAVKDGGAFENKDGDKGTFLRVRENKDLRLTFETSGSSAPTQVDV
                     QFQDKGKGKTGLLVNHTRIQTREEADGLRAAWADALDKLKTTCEA"
     gene            2888..>3851
                     /locus_tag="VFV61_07105"
     CDS             2888..>3851
                     /locus_tag="VFV61_07105"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX4900285.1"
                     /translation="MNIESFHPAVARWFAKTFPAATAPQQLAWPSIKSQRDTLIAAPT
                     GSGKTLAAFLAAIDHLVRLGVAGELDDRTHVVYVSPLKALSNDIQRNLQTPLEGIRQE
                     LRAMGLPEINIRTLVRTGDTPAAERTAMVKRPPHIVVTTPESLYILLTSEGGRRMLQT
                     ARTLILDEIHAVVGDKRGSHLALSVERLADLSARHESENSKPETRNSKLVRIGLSATQ
                     RPIEEVARFLVGTDKVKADGTPDCAVIDTGHARQLDLAIEVPESPLQAVMSGEVWEEI
                     YDRLAELIRQHKTTLVFVNTRRLVERVSRHLGERLGDENIAAHHG"
BASE COUNT          943 a         1028 c         1043 g          837 t
ORIGIN      
        1 ctttcccgaa ggggcgaact cggtaggctg tcatcaacta attggtgacg tttgggagtg
       61 gacaacttcc gattacgtgc cctatcccgg attcaaatcg gagtttgatg agtacaacga
      121 caagtggttt gtaaatcaaa aagtgctgcg cggaggttca tttgccacgc cgcaacgtca
      181 catccgctcg acctaccgca acttcttcca tgcgcacgaa cgctggatgg tctcaggctt
      241 tcgctgtgcg aaggatctgt aacgcaaaca agaagggaac atgcctactc ccccagatac
      301 agaatcccga accgccgtta aggagttttt actcgccgaa taccacaacg tcgccgattc
      361 gttttggaaa aatgaacaga ccggtgaaac acgagttaac tggttcattg gcattgtcac
      421 tgccgccgtg ggcggcttgt ttggtctaac cactgcggaa aagcgcccgc atggcgaacc
      481 gttacgcctc atctatctcg ccgctttgtt cgccctcctg tctttcggcg tcgtcaccct
      541 gctcaggatg atgaaacgca atgaaactac cgatggttat aagaaagacg ggaagagaat
      601 ccgccgcctg tttgctgcat ccttcgacga cgccggcatc ctccgcaact atggctcatt
      661 cggcgacggt agtcagccaa aggaaactct acgaaagctt ggcggccttt cgcacacagt
      721 ttcaaccatc aatagcttgt tgctggcggg aatcgccgcc gcggcggttt acccctttgg
      781 tattagcgat gaaagcaaac gtgtcgcgca gatccttctg acatatagaa tttctccttt
      841 ggtgtttatc ctggcctttg ttggtcagtt cttctggatt agacgggcgg aagaaaaatc
      901 caggcaacga gttcgcacaa caactcacgc gggcggaatt gtgttcagcg acgaaggagg
      961 caaggtgaac tacctgcttg tgcgtccaag caagaaaaag ccagacaaag atgaatggct
     1021 gtttccgaag ggtcacatca aagaaggtga agagcaatgg gaaagtgccg tccgcgaagt
     1081 tcgcgaggaa gccggtgttg ttggccattt gatttgcccg gtcggaatag atgcctacga
     1141 ggtagaagag aaacctctgc aagtaaaatt ctatttgctt agatccgagg caatagttcc
     1201 gcgcacagag gatcgcgaaa taacgtgggt tgccttcaca gccgccttaa aaaatcttag
     1261 ctatctgggc agcaagcaat tgctggtcaa ggctgagtta aagcgtcgcc tagcgcaaaa
     1321 ggagtaccga cgcagttgac gacaattctt tcggacattg aagtgcgcgt cttgggtgcg
     1381 ctcgtcgaga aacaggtgac cacgcccgag tactacccgc tcaccttaaa ttccctcacg
     1441 cttgcttgta atcagaagaa caatcgtaat ccggtcactg gttactcaga aaatgaagtc
     1501 gcccaggcgc tggagacatt aagggaaaag aatctcacct acgtctttca cggcagtacg
     1561 agccgcgtgc ccaagtacaa gcacgtgatg cccgaggtgc tgcatctaac cccgcccgaa
     1621 accgccttgt tgtgtgtctt gatgttgcgc gggccacaaa cgattggtga gctgcgtggc
     1681 cacggcgctc gcttgtacac cttcactagt cttgatgaag ttgacgccac tgtgaatgcc
     1741 cttatcactc acgaccctga tccaatggtt gttcgtttgg cgcgccagcc gggacagaaa
     1801 gaggcccgtt tttctcactt gctcgccggt gtacctgcgg aagacctcgc ttctgacact
     1861 ccggcaccaa ggccaagttc atcaagacat ggtgacaggg aacggattga gcagttggaa
     1921 cagaccgtca ctgccctgag ggaagaaata caagcattga aacaacagtt tggagagttc
     1981 aagaaacagt ttgagtgact gactacggac tacggaccac tgagggttaa aggatttcgc
     2041 acttacgttt aatagaagct taagaacaga atcccgaagg agagctgcga atgaagagta
     2101 ccttcaaatc ggatcacccc gtaactaatg aagccgccaa gactgccacc ggcaaaaccc
     2161 tcgaccagtg gtttgccgaa ctcgacaagg aaaatggtct gaaacttggg cgccgtgaga
     2221 ttaacaaccg tctttacgca cagaagctcg atccctggtg gtgcgtaacg atcgcagtcg
     2281 agtatgagaa acatcacgat gtgcgcaaaa aggatggcct gttcgagggt tattttgtct
     2341 gcgcaaccaa gacgattggc tctccgcccg ctgctgtctt taaggcgtgg tcgagcggtg
     2401 cagagttgag caagtggttc ggaccagcca ctaaagccgc agtcaaagat gggggcgcgt
     2461 ttgaaaacaa ggatggcgac aagggaacgt ttctgcgcgt gcgcgaaaac aaggacctgc
     2521 gcctgacgtt tgaaacgagc ggctcgtcag caccgacgca ggttgatgtg cagtttcagg
     2581 acaagggcaa aggcaaaact ggcttgctcg tgaatcacac gcgcattcaa actcgcgaag
     2641 aggcggacgg tctgcgtgca gcttgggcag atgctttgga taagctgaaa accacctgcg
     2701 aggcttgatt cggaaaccgt tcaaacggtt tcttggatcg ccgaccaatc tcctcctggc
     2761 taaagccagg taagaatgaa aggggcagtg ccgtctgccc actgccgacc gttttggcag
     2821 tggcccatgt ctaggccgct gtttcatcta tgcaaggagg gcgaggtttt gtataatctc
     2881 gcttcctatg aatatagaaa gttttcatcc agccgtggcg cgttggttcg ctaaaacctt
     2941 ccctgctgct actgcgcccc aacaactcgc ctggccgtca atcaaatctc agcgcgacac
     3001 gctcatcgcg gctcccaccg gttccggcaa gacgctcgcg gcgtttttgg cagcgattga
     3061 tcatttggtg cgactcggag tcgccggtga gctcgacgat aggactcacg ttgtttacgt
     3121 ctcgccgtta aaagccctca gcaacgacat ccagcgcaac ctgcaaactc cgctcgaggg
     3181 aattcgacag gagttgcgag caatgggatt gcccgagatc aacattcgca cgctcgtgcg
     3241 cactggcgac acccccgctg ccgagcgcac cgcaatggtt aagcgcccgc cgcacatcgt
     3301 tgtcactacg ccggaatcgc tttacatcct gttgacgagt gaaggcggcc gccgcatgtt
     3361 gcaaaccgcg cgcacgttga ttctcgatga gattcatgcc gtagtcggcg acaaacgagg
     3421 ttcgcatcta gcgctttccg ttgagcgcct cgccgatctg agtgcgcgcc acgaaagcga
     3481 aaactcgaaa cccgaaactc gaaactcaaa actggttcga atcggtttgt ctgcgaccca
     3541 acgtcctatt gaggaggtgg cgcgctttct ggttggtacc gataaggtca aggccgacgg
     3601 taccccagac tgcgcagtca tcgataccgg gcatgcacgg caactcgatc ttgcgatcga
     3661 agttccagag tcgcccctgc aagcggtcat gtcgggcgaa gtgtgggagg agatctacga
     3721 tcgacttgcc gaactaattc gccaacacaa aacgacactc gtttttgtta acacccgtcg
     3781 tcttgtcgaa cgcgtgtccc gacacttggg agagcggctc ggcgacgaga atattgcagc
     3841 gcatcacggc a
//
LOCUS       DASUEX010000419         2790 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_996873, whole genome shotgun sequence.
ACCESSION   DASUEX010000419 DASUEX010000000
VERSION     DASUEX010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2790
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_996873"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1416)
                     /gene="ppsA"
                     /locus_tag="VFV61_07110"
     CDS             complement(<1..1416)
                     /gene="ppsA"
                     /locus_tag="VFV61_07110"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: protein motif:HMM:NF005057.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HEX4900286.1"
                     /translation="MVKSSKPLVLKLSEVGAGDVELVGGKCASLGELFRALVPHGVRA
                     VDGFATTSEAYRLLVNTNGLGQRLRELMAGLDYNDMKALNEAGKEARALMLDTPLPQE
                     VNDAILNAYHELCERLGRTPEVAVRSSATAEDLPDASFAGQQDTILNVRGEAHLIEAC
                     HRCFASLWTDRAISYRAARGFDHFDVALSIGVQPMVRSDQACSGVMFSLDTESGFRDV
                     VVINGAWGLGEAIVQGMATPDEWVVFKPTLKTGARPIVSRRLGGKEVKMVYGLDGRGT
                     RTREVVEAQRNRFVLGDYEVLELARWACKIEEHYSQLAGKHTPMDIEWAKDGYTGELF
                     ILQARPETVHASNRENYIETYRLTGEHGEPLVSGIAVGEKISHGRVHVLKDPDHLTDF
                     NAGDVLVTSMTDPAWEPIMKKAAAIVTDRGGRTCHSAIISRELGLPCIVGTGNATQLL
                     QSGTDVTVSCSEGAHGNIYEGL"
     gene            1871..>2790
                     /gene="glpX"
                     /locus_tag="VFV61_07115"
     CDS             1871..>2790
                     /gene="glpX"
                     /locus_tag="VFV61_07115"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HEX4900287.1"
                     /translation="MGHEIRTDRELSLDFLRVCEAAAIASARTMGQGDRKYSDHVAVE
                     AMRETMDTVPMRGRIVIGEGERDEAPMLYIGEEVGGGFGVGEELADSCPEVDIAVDPL
                     EGTNLCALGANNAIAVLAAAERGGLLNAPDIYMDKIVVGPSSRGVVDLDAPVKENLKN
                     IARRLGRDVEDLTVIVLDRSRHKKLIADVREAGARIRLIGDGDLSAGISAAVAGTNIH
                     ALLGIGGAPEGVITAAAMKCLNGEIQARLVFDPERLGVDKDKVPAKEAVLERLRSMGI
                     NDPDKIYDTNDLAPGKKIIFAATGVTDGALL"
BASE COUNT          583 a          777 c          800 g          630 t
ORIGIN      
        1 tagcccctca taaatgttgc cgtgcgcgcc ttctgagcag gaaaccgtga cgtcggttcc
       61 gctctgcaga agctgcgttg cattgcccgt accgactatg cagggcagtc ccagctcgcg
      121 gctgatgatc gcgctatgac aagtacggcc accgcggtcg gtgacgattg ccgcggcttt
      181 tttcatgatc ggttcccagg cgggatcggt catggacgtc acgagcacgt cgccggcatt
      241 gaaatcagtt agatgatccg gatctttcaa aacgtgcacg cgaccgtgac tgatcttctc
      301 gccaacggcg atgccggata cgagtggctc gccatgctca cctgtaagcc ggtaggtttc
      361 gatgtagttt tcacgattgc tggcatgaac agtttcggga cgtgcctgga gaataaagag
      421 ttcgccggtg tagccatcct tcgcccactc gatgtccatc ggcgtgtgct tccctgctaa
      481 ctgcgagtag tgctcctcaa tcttgcaggc ccagcgcgcc agctccaaca cttcatagtc
      541 gcccagcacg aagcgattcc gctgcgcttc gactacctcg cgagtccgag tgccccgacc
      601 gtccaaaccg taaaccatct tcacttcttt accacccagg cgtcggctaa caatcggacg
      661 agcacccgtt ttcagggttg gcttgaacac cacccactcg tcaggggtcg ccataccctg
      721 cacgatggct tcacccaaac cccaggcgcc gttaatcacc accacgtcac gaaaaccgga
      781 ttcagtatcg agactaaaca tcacgccgga acacgcctga tcggaacgca ccattggttg
      841 cacgccgatt gaaagcgcta cgtcgaagtg atcgaagccg cgcgccgctc gatacgagat
      901 cgcgcggtcg gtccaaagcg aggcgaagca ccggtggcaa gcctcgatga gatgggcctc
      961 gccgcggacg ttcagaattg tgtcttgttg gccggcgaaa gaagcatcgg gaagatcttc
     1021 ggctgtggct gaagaccgaa ccgcaacttc cggcgtgcgt cccaggcgtt cgcagagttc
     1081 gtgataagcg ttgaggattg catcgtttac ttcctggggt agcggggtgt ccagcatcaa
     1141 agcgcgagct tccttaccgg cttcgttcag cgccttcatg tcgttgtagt caaggcctgc
     1201 cattaactcg cgtagtcgtt gtcccaaacc attggtgttc actagcagcc ggtacgcctc
     1261 actggtagta gcaaagccat caaccgcccg cacgccgtgg ggcacgagag cgcgaaacag
     1321 ttcgcccaga ctggcgcact tgccgccaac gagttcgacg tcaccagcac caacttcgct
     1381 cagtttgagg acgagaggtt tggaactctt aaccatattt tctcctgcac tcttgtttgg
     1441 tggaaaacgc tttgcccgac aggttgccgc aacggaaccc cattctagtt ggttgccttg
     1501 gagcttgaga agctcgatct cgcagcacct atatctaaaa acgacgaatt gacccttccc
     1561 tacagctttg ggcggaaatt ttcactgcct ggtttgatcg gctggcggcc cgggtttcat
     1621 tggtctttga aacgaactgc tggtaagtca aacccgagtg ccggtcgaat gaaatcccgc
     1681 tctttcgcct tggtcgctcc gcctcccggt ttctaacgcg agtcgaatga tttgtgccgg
     1741 ttacggtccg cttgccgctg ctacaaattc ccgcactgct ctgttagtat gagcgcgcac
     1801 ttcatccatc tgcgggcagc acttctttta ctgtttcgcg gtcaaaattc cgaatcagga
     1861 gagctatgtt atgggtcacg aaataaggac agatcgcgag ctgtctctcg atttcctgcg
     1921 agtttgtgaa gcggcggcca ttgcctccgc acgcacgatg ggccaggggg atcgtaagta
     1981 ctccgatcat gtagccgtcg aagcaatgcg ggagacaatg gataccgtgc cgatgcgcgg
     2041 tcgcattgtc atcggcgaag gcgagcgcga tgaagcgccg atgctttata tcggtgaaga
     2101 agtcggtggc gggttcggtg ttggtgaaga gctggccgac tcgtgcccgg aagtagacat
     2161 tgccgtcgat ccacttgagg gaacaaatct gtgtgcgctc ggggccaaca acgcaattgc
     2221 ggtgctggcc gcggcggaac gcggtggttt gcttaacgcg cccgacattt acatggacaa
     2281 gatcgttgtc ggaccttcgt cacgtggtgt tgtcgacctt gatgcgcccg tgaaagagaa
     2341 cctcaagaac atcgcgcgac gcctgggtag agatgttgaa gacttgaccg tgatcgtgct
     2401 tgatcgctcg cgtcataaga agttgattgc tgacgtgcga gaggcaggtg ctcgtattcg
     2461 gttgatcgga gacggcgatc tgtcagccgg aatttcggcg gcggttgcgg gaaccaacat
     2521 tcatgccctc ctgggaatcg gtggcgcccc ggaaggtgta attacggcgg ctgcgatgaa
     2581 atgtcttaat ggcgaaatcc aggcgcgtct ggtttttgat ccagaacggt tgggcgtgga
     2641 taaagacaag gtgccggcga aagaagcagt tctggagcgc ttgcgatcga tgggcattaa
     2701 cgatcccgac aagatttacg atacgaatga ccttgcgcct ggcaaaaaaa taatctttgc
     2761 tgccaccggc gttactgatg gcgcgttgct
//
LOCUS       DASUEX010000420         3170 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_881537, whole genome shotgun sequence.
ACCESSION   DASUEX010000420 DASUEX010000000
VERSION     DASUEX010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3170
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_881537"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1876)
                     /locus_tag="VFV61_07120"
     CDS             complement(<1..1876)
                     /locus_tag="VFV61_07120"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEX4900288.1"
                     /translation="MPVRYPPPDSVPPVIRGRAVSPRKIVAYEQPGTSFPRRVGRRFL
                     SPFILIPLAVCFVIVGGVLVYYWSIFSGRIDNLLRGDVYTRSAGIYAAPKQLRPGESI
                     SAEDLVAYLKRAGYVEKGTQAETARGRFSIQGSNVDIEPSQNSSVDGVQQFPRIRAQF
                     AKSGKAVSSISNLDGNGRLERAWLEPELISSVTGRERAKRKIIGFNDLPPHLVKAIVV
                     TEDRSFFEHYGVNLRGIVRALIRRYDNSDPNSPIARQGGSSITQQLVKNLLLSPERTL
                     RRKVAEAYMSIILETRLTKEKIFELYCNQVYLGQQAGFSINGFGEAADAYFGKDVTNL
                     TLPEAALLAGLIRSPNRYNPYHEVETATSRRNQVLDSMAETGAITRDEADRAKAEPVK
                     VAAVRGRIDASDAPYFSDYVQSQLGDIISGPGSAEHLRIYTTIDMDLQRAAYSALSKQ
                     VAALDKVQAKRFAPGTLQAALVALNAKTGEIVAMVGGRDYSKSQLNRAASALRQPGSV
                     FKPFVYATALNTAYDPVPRVITPATVYIDEPKTFTFDNQEYSPGNFGETYRHGPVTLR
                     DALVNSLNVITVDVAMEVTIGRVMALAAKAGLPKPARAYPAMALGTSEATPLQVASAY
                     T"
     gene            2010..3035
                     /locus_tag="VFV61_07125"
     CDS             2010..3035
                     /locus_tag="VFV61_07125"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel protein"
                     /protein_id="HEX4900289.1"
                     /translation="MSLPPPRSSRFADKGFRIRYAVLAVLVAIAVGTLGFHLIEGWPV
                     LTSLYVATQTVTTVGYGDVAPQTLLGRVFATAFMLAGVGVVLYALTSTVQSIVQSELL
                     ATFGQRRRFRKMSKLKNHFIVCGAGRVGSRLVRSLIEAGEDFVVIETDPDKLTELAES
                     GVATLARDATLEATLREAGVTRARGLAACLPDDADNVYVVLTARDLNPQLHIVARAAE
                     EQAEAKLIRAGANRVVAPTILGGHRLAVALTKPAVGDFIDSLTVNTLGLVFEQLTVGA
                     SSLVAGKKLKDTNIRSELDIVVVSIQRCDGEMVFNPSGEAVIAAGDILIAIGKAESLM
                     KLTAMAK"
BASE COUNT          690 a          864 c          910 g          706 t
ORIGIN      
        1 cggtgtaagc cgaagcgact tgcagtggcg tggcctcact ggttcccagc gccattgccg
       61 gataggcgcg cgcgggttta ggcaagcccg ctttggcggc gagcgccatt acacgaccga
      121 tcgtcacttc catcgccaca tcgaccgtta ttacattgag actgttaacc aatgcatcgc
      181 ggagcgtcac cgggccgtgc cgatacgttt cgccgaagtt accaggcgag tattcctggt
      241 tgtcgaaagt gaaggtcttg ggctcgtcaa tgtagacagt tgccggggtg atgacccgcg
      301 gcaccggatc gtaggccgtg ttaagtgcgg ttgcataaac aaacggcttg aaaactgaac
      361 ctggttggcg tagcgccgat gccgctcgat tgagctggct cttggaatag tcacggccac
      421 cgaccatggc cacgatttcg ccggtcttcg cattcagggc cacaagcgca gcctgcaatg
      481 tccccggcgc aaaacgcttc gcctggacct tatcgagagc tgcgacttgc ttcgaaagtg
      541 cgctgtacgc ggcgcgctgc aagtccatgt caatcgtggt gtagatccgc aaatgctctg
      601 cagaacccgg accggagatg atgtcgccaa gctggctctg cacgtaatcg gaaaagtagg
      661 gagcatctga agcgtcgatg cggccgcgca cggcggcaac cttcacaggc tctgccttgg
      721 cgcgatcggc ttcatcgcgc gtaatcgctc ctgtctctgc cattgagtcg agtacttgat
      781 tgcgccgcga ggttgcagtc tcgacctcgt gataaggatt gtagcgatta gggctgcgaa
      841 tcagaccggc cagcagcgca gcctccggca atgtcaggtt cgtcacatcc ttgccgaagt
      901 aagcatcagc ggcctcgcca aaaccattga tcgaaaagcc cgcctgttga cccagatata
      961 cctggttgca gtagagctcg aaaatctttt ccttcgtcag cctcgtttcg agaatgatcg
     1021 acatgtacgc ttccgcaact ttacgacgca gggtgcgctc gggtgagagc aggaggttct
     1081 taactaactg ttgagtgatg ctcgaaccac cctggcgagc aatcggcgaa ttaggatcag
     1141 agttgtcata gcgacgaatc agagcgcgca cgatgccgcg cagattgacg ccgtaatgtt
     1201 caaagaagga tcgatcctcg gtgactacaa tcgcttttac cagatgcggc ggcagatcat
     1261 tgaagccaat aatctttcgc ttggcgcgtt cgcggccggt taccgagcta atcagttcag
     1321 gctccagcca ggctctttcc aaccgaccat tgccatccag attagaaatt gaacttactg
     1381 ccttgccgct cttcgcaaac tgcgccctaa tccggggaaa ctgctggaca ccgtccacac
     1441 ttgaattctg acttggctca atatcaacat tcgaaccttg gattgaaaac cggccgcggg
     1501 ctgtttctgc ttgagttccc ttctcgacgt agcctgcgcg tttcagatac gcgactaagt
     1561 cctccgctga gattgattct ccgggccgga gctgtttggg tgcggcatag atgccggctg
     1621 agcgggtgta gacgtcgccg cgcagcagat tgtcgatgcg cccggaaaaa atcgaccagt
     1681 agtagactag aacgcccccg actatgacga agcagacagc gagtggaatg agaatgaagg
     1741 gacttaggaa gcggcgtcca acccgacgcg ggaaactggt gccaggctgc tcgtaagcta
     1801 cgattttgcg gggagaaacg gcgcggccgc gaatgactgg cggcaccgaa tcaggaggtg
     1861 gatagcgtac gggcatagtt tagcggtcgg tggtcaattg cgagtaccta cttcttttta
     1921 tcacccggcg cctgtaaatt gacagcccca atgtctttgg ctaccgtgac agcgcttcgg
     1981 cactgcctct ttgatgctgc tccctacata tgagtctacc acccccgcgg tcgagtcgat
     2041 tcgccgacaa agggtttcga attcgctatg ccgtgctggc tgttctcgtt gccatcgcag
     2101 tcgggacgct gggatttcat cttattgaag gatggccagt cctcaccagc ctctatgtcg
     2161 ccacacaaac ggtgaccacg gtgggatatg gtgacgttgc accacaaacg ttgctcggtc
     2221 gcgtttttgc cactgctttc atgttggctg gggtcggcgt agttttgtat gccctgacct
     2281 cgacggtgca gtcgatcgtg caatcggaat tgctcgctac cttcggccaa cgccgccgct
     2341 ttcgcaaaat gagtaaacta aaaaatcact ttatcgtgtg tggcgccggg cgagttggat
     2401 cgcgcctggt acgtagtcta atcgaagctg gagaggattt tgtcgtcatt gaaactgatc
     2461 cggacaagct tacggaattg gccgagtcgg gcgtagcgac gctcgcccgc gatgccacgc
     2521 tggaagctac tctgcgcgaa gctggggtga cgcgagcccg gggactggct gcttgcctgc
     2581 cggacgacgc tgacaatgtt tacgttgtat tgacggcgcg cgatctgaat ccgcaacttc
     2641 acatcgtggc acgcgcggca gaagaacagg ctgaagcaaa gctgattcgc gccggggcaa
     2701 atcgagtggt ggcgccgacc atcctcggtg ggcatcggct ggcggtcgcc ttgactaaac
     2761 ctgctgtagg agactttatc gactcgctca cagtaaacac tctcggactg gtattcgagc
     2821 agctcacagt cggagcaagc tctttagtgg cggggaaaaa gttgaaagac accaacatcc
     2881 gttcggaact cgacattgtg gtggtcagca tccagcgctg tgatggtgag atggtcttca
     2941 atcccagtgg tgaggcggtg atcgctgcgg gcgacatctt gatcgcaatt ggaaaagcag
     3001 agtcgctaat gaagttgacg gcgatggcca aataaaccaa ctttcaagtt ctaactatga
     3061 tttgacctct tagatcggcc aacgtcggcg ctgccggaac tcgaaacctg aaaccctgaa
     3121 acctgagacc cgaaactcga aaccgacttt gtaatgcgta ccttagttac
//
LOCUS       DASUEX010000421         2631 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1116477, whole genome shotgun sequence.
ACCESSION   DASUEX010000421 DASUEX010000000
VERSION     DASUEX010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2631
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1116477"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..832
                     /locus_tag="VFV61_07130"
     CDS             <1..832
                     /locus_tag="VFV61_07130"
                     /inference="COORDINATES: protein motif:HMM:NF027219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative zinc-binding metallopeptidase"
                     /protein_id="HEX4900290.1"
                     /translation="DGVPGIAIPFYLAHPRLMKLERKQMLEVEGGTDPECMRILRHEA
                     GHALDTAFRLHFKRKWRELFGSFARPYPDFYKPKPKSRNYVLHLPAWYAQAHPAEDFA
                     ETFAVWLTPRSRWRRRYKGWPALRKLEYIDELMQELAGTKPRNRLRTKVEPLSALKIT
                     LREHYRRKREKYDFDWPAVYDRDLKRIFSEDPRHKSKLSAAIFLRSVRREVRQIVAEG
                     TGVHQYTIDHVLANMIDRCKDLKLRVASPPREARRRVMIVLTVQVMNVLHSGYHRIAV
                     "
     gene            829..1866
                     /locus_tag="VFV61_07135"
     CDS             829..1866
                     /locus_tag="VFV61_07135"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900291.1"
                     /translation="MTFEPKKKLRIIVLVHEDLVPPDSLTGLSDKEKIEIKTEYDVIS
                     TLKKMGHEVYPIGLYNQLNVIGNALMEHKPHVAFNLLEEFHGYPLYDQHVVSYLELMK
                     QAYTGCNPRGLTMAHDKALTKIILSYHRIQVPGFAVFPLNRKVRRPKRLKFPLLVKSI
                     SEEGSVGIAQASIVHDDDKLAQRVDFIHRQNKTAAIAEQYIKGREIYVGLIGNQNIQT
                     YSPWELVMEKLPEGAENIATLKVKWDPTYQEKVGVATRAAELTPEQHKTLERLSKRIY
                     RYLFLSGYARLDYRMTEEGQFYLLEANPNPQIAEGEDFADSAAHSGLPYDQLLQKIIT
                     VGLNYHAGQAA"
     gene            2275..>2631
                     /locus_tag="VFV61_07140"
     CDS             2275..>2631
                     /locus_tag="VFV61_07140"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HEX4900292.1"
                     /translation="MTLRNWFPYTIAACLTLSIFSVAVVAQTGETRQRQIASTDQALN
                     HVALNDDPVVLSVASPADIKAASLGTPSLASAKFQQLMLAAIDQRLGARYSWGAEGPS
                     AFDCSGFVWSTFQSVGI"
BASE COUNT          606 a          710 c          679 g          636 t
ORIGIN      
        1 tgatggcgtt ccgggcatag caattccgtt ttacctggcg catccgcgtc tgatgaaact
       61 cgaacgcaaa cagatgttgg aagttgaggg cggcaccgat ccagagtgca tgcggattct
      121 ccgtcacgag gcggggcacg cgctcgacac tgcttttcgt ttgcacttta agcggaaatg
      181 gcgcgagctc tttggttcat tcgcccgacc ctatccggat ttttacaaac ccaagccaaa
      241 gagccgcaac tacgtgctgc atctgccggc ctggtacgcc caggcgcatc ccgccgagga
      301 tttcgccgag acgtttgcgg tctggctcac accacgttcg cgttggcgcc ggcgctacaa
      361 aggctggcca gccttgcgca aacttgaata catagacgag ctgatgcagg agcttgccgg
      421 caccaagccg cgcaatcgtt tgcgcacgaa agtcgaaccg ctctcggctt tgaaaattac
      481 tctgcgcgag cattaccgcc gcaaacgtga gaagtatgac ttcgactggc cggcagttta
      541 tgaccgtgat ttgaaaagaa tcttttcaga agatccgcga cacaagagca aactaagcgc
      601 ggcgattttc ctgcgcagtg tgcgtcgtga agtgcgtcaa atcgtcgccg aaggaaccgg
      661 agttcaccag tacaccatcg atcacgtcct cgcgaacatg atcgatcgct gcaaagatct
      721 gaagttgcgt gttgccagtc cgccgcgcga ggcccggcgc cgcgtcatga ttgttttgac
      781 agttcaggta atgaacgtgc ttcattcagg ttatcatcgc atcgccgtat gacgtttgaa
      841 cccaaaaaga agcttcgcat catcgttttg gtccacgagg atctggtgcc gccggattcc
      901 ttgaccggcc ttagcgacaa agaaaagatc gagattaaga ctgagtatga tgtgatctcg
      961 acgctcaaga agatggggca tgaagtgtat ccgattggtt tgtataacca gttgaacgtg
     1021 atcggaaacg ctttgatgga acacaagccg cacgtggcgt tcaatctgtt ggaagagttt
     1081 cacggttacc cgctttatga tcagcatgtc gttagttacc tcgagttgat gaagcaggcc
     1141 tacaccgggt gcaacccgcg cggtttgacg atggcacacg acaaggcgct gactaaaatt
     1201 attctttcct atcaccgcat ccaggttccc gggttcgcag tgtttcccct caatcgcaaa
     1261 gtgcggcggc cgaagcgtct gaagtttcct ttactggtca aatcgattag tgaggaaggc
     1321 tctgttggta tcgcgcaggc gtcaatcgtt catgacgacg ataagctggc gcagcgagtt
     1381 gacttcatcc atcggcaaaa caaaacagcg gccatcgccg aacagtacat taagggccgc
     1441 gaaatttacg ttggcctgat cggtaatcaa aacattcaga cctattcccc ctgggaattg
     1501 gtgatggaga agctgcctga aggcgccgaa aacatcgcaa cgctgaaagt gaaatgggat
     1561 cccacttacc aggagaaagt tggagttgcc acccgggctg ccgaacttac gccagaacaa
     1621 cataagacgc tggaacgact ctctaaacgc atctatcgtt acctgtttct gagtggttac
     1681 gcccgccttg attaccggat gacggaagaa ggtcagtttt acttgcttga agcgaatccc
     1741 aatccgcaaa tcgcggaagg tgaagatttc gctgattcag cagcccattc aggtcttcct
     1801 tacgaccagt tgctccagaa aatcattact gtcgggttga actatcacgc gggtcaggca
     1861 gcctagcacc acctcacgtc tgggaatcgg cgtctattcg tcctttctct tcttcgtaca
     1921 cacaattcaa gttcgatttt ttccaatgac tctgggactc accctccttt ttgccctgac
     1981 tctctatcgg cacgtatccc gcagccgaag tcagtgatct tgtcatcaca ttgaccgtag
     2041 tgtcttgtgg tgaaacatct ggcctcgatt tctcggacct ctctccatct aagaacgcag
     2101 gccgtgaacc ctttcggtgc gctggcggtg aacctgatgt gcttcgtcac gaaacagtca
     2161 cattggttct tgtggaacct ctctccgctg gctacactcg cttagctttc aggtagtggc
     2221 cccctcactc agtaaagaca aaagaaaaat ttaggagagg ttgttcccct ttctatgact
     2281 ctacgaaact ggttccccta cacgatagcc gcgtgtttga ccctcagcat tttctccgtc
     2341 gcggttgttg cgcagaccgg cgaaactcgc caacgccaaa tcgcctcaac agatcaagct
     2401 ttaaaccatg tcgccttaaa tgacgatccg gttgtgctgt cggtggcatc gcctgccgac
     2461 attaaggctg catcgctggg tacgccctcg ctggcttcgg caaaatttca gcaattgatg
     2521 ttagcggcaa tcgatcagcg actcggcgcg cgttattcat ggggcgcaga gggaccttcg
     2581 gcgttcgatt gctcgggatt tgtttggagc acgttccaat cggtgggtat c
//
LOCUS       DASUEX010000422         2091 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_882231, whole genome shotgun sequence.
ACCESSION   DASUEX010000422 DASUEX010000000
VERSION     DASUEX010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_882231"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /locus_tag="VFV61_07145"
     CDS             complement(<1..541)
                     /locus_tag="VFV61_07145"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEX4900293.1"
                     /translation="MRRGGTWIQRWRVPLGFLCAGAFIVLSRPRPLTILLGGLVSLLG
                     LAIRAWSAGHIRKNEALAVSGPYAFTRNPLDLGSFVLGLGFTIGSGRWELAIVFAALF
                     LGIYLPVMRVESRTLAEMFGEDYRQYALQVPLFFPRLKRYDDGTAAPMGFDRSLYLRY
                     REYRAALGLFAAWVALAIKA"
     gene            complement(920..1474)
                     /locus_tag="VFV61_07150"
     CDS             complement(920..1474)
                     /locus_tag="VFV61_07150"
                     /inference="COORDINATES: protein motif:HMM:NF013621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HEX4900294.1"
                     /translation="MENQSRVLQLSELLARVKARREQGARVVLANGCFDVLHVGHVRY
                     LEGAKAEGDLLVVGINSDKQVATLKGAGRPLMGEQERAEIVAALEAVDMVTIFDEPTV
                     TELLLALKPDVHAKGTDYTEDTVPEREVVRSYGGRVAIVGDPKNHSTSQLIGVMAASR
                     RVAESKNAEERAEVSSSEVAVDSD"
     gene            complement(1480..>2091)
                     /locus_tag="VFV61_07155"
     CDS             complement(1480..>2091)
                     /locus_tag="VFV61_07155"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX4900295.1"
                     /translation="DYEGTPPASRQLHAQLRERAVEAANSAAAVVISDYNYGVANAEM
                     ADALRPLALSKKCPVLVDSRFELSRFNGFTSATPNEDEVEQLLKMNGNFADLESAGER
                     LRGGLGYRALLITRGGHGMILLEDGVAPLHLAAVGGSEPVDVTGAGDTVMATYSLALA
                     AGASFSDAAHLANHAGGIVVMKRGTAAITPGELEHSIVNADAN"
BASE COUNT          463 a          639 c          575 g          414 t
ORIGIN      
        1 aagcttttat cgccagggca acccaggcag cgaacaaacc cagcgccgcc cgatactcac
       61 gatagcgtaa gtaaaggctg cggtcgaagc ccatcggggc tgcggtgccg tcgtcgtatc
      121 gtttcaagcg gggaaagaag agcggaacct ggagggcgta ttgacgatag tcttctccaa
      181 acatttccgc cagtgtccgc gattcgacgc gcatcaccgg caaatagatg ccaagaaaga
      241 gagcggcaaa gacgatggcc agctcccagc gaccggagcc gatcgtaaaa ccgagaccca
      301 gaacgaaact gccaaggtcg agcggattgc gagtaaacgc ataggggccg gagacagcca
      361 gggcctcatt ttttcggata tgtccagctg accaggcacg gatagccaga cctaataggg
      421 aaactaaacc accaagcaaa atggttaggg gacggggtcg tgagaggaca atgaatgctc
      481 ccgcgcaaag gaaacccaac ggcacacgcc agcgctgaat ccaggtacca cctctacgca
      541 tctgtgctca acaagtcctt cggcgttccg tctcgcctag atgtcaggag tcaaaaagaa
      601 ggcgcgccga agtggcgcgc ctgctgatag tgccaaaaat aatcgaaggc gaaaggctcg
      661 taaccgacgt aactgctggc gcggtgcccg cagcgtctcc ccagacatca gtctcgcgcc
      721 tcagcgcctt cgtgccgact acgatgaagc gatttgaatg cgggttgtct cttttttttt
      781 cgaacccgat cccctgctta taattctgcc ctggtcgctc cgcaaccgac ctgtctaaag
      841 agctggactg cgtggtaacc gagggtgacg cacgataaac aagcgatttt cttgaaggat
      901 tcatcaatcc ccgcattgct taatcagagt caactgcaac ctcagacgag gagacctctg
      961 cccgctcctc cgcattcttc gactcggcta cccgccggct cgccgccatc actccgataa
     1021 gttgcgatgt tgaatgattt tttggatcgc caacgatagc cacgcggcca ccgtagctgc
     1081 ggacgacctc gcgctccggg actgtgtctt cagtgtaatc ggtgcccttg gcgtgcacgt
     1141 cgggtttgag cgccagcagg agttccgtca ctgttggttc gtcaaatatt gtcaccatgt
     1201 caaccgcctc gagcgccgca acgatctcgg cacgttcctg ctcgcccatg agcggccgtc
     1261 cggcaccttt caatgttgcg acctgcttat ctgagttgat gccaaccacc aggagatccc
     1321 cttctgcctt ggccccctca agatagcgga cgtgtcctac gtgcagcaca tcaaaacaac
     1381 cattcgcgag caccacacga gcgccttgct cgcggcgggc cttgacccgc gccagcagtt
     1441 cgctcagttg cagcactctc gactgatttt ccatgactat taatttgcgt ctgcgttgac
     1501 gattgagtgt tccagttcac caggagtgat tgctgccgtc ccgcgtttca tcactacgat
     1561 tccgccggcg tgattcgcca gatgagcggc atctgagaat gaagcaccag cggcgagggc
     1621 taatgaatag gtagccatca ccgtgtcgcc ggctccggtg acgtccaccg gctcgctgcc
     1681 gccgaccgcg gcaaggtgca aaggcgcaac gccatcctcc agaaggatca tgccgtgccc
     1741 accgcgcgtg atcagaagcg cgcgatatcc caaacccccg cgcaggcgtt caccggcaga
     1801 ctccaggtcc gcgaagttgc cattcatctt cagcagttgt tccacctcat cttcgttggg
     1861 agtggcggaa gtaaaaccgt tgaagcgcga gagttcaaag cgggaatcaa ccagcactgg
     1921 acatttcttt gagagcgcaa gagggcgcaa ggcgtcagcc atctccgcgt ttgccacgcc
     1981 gtagttatag tccgagatga ccaccgccgc cgcactgttt gctgcctcga ccgcgcgctc
     2041 gcgcagttga gcgtgcaact gccggctggc tgggggagtg ccctcgtagt c
//
LOCUS       DASUEX010000423         9726 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1208542, whole genome shotgun sequence.
ACCESSION   DASUEX010000423 DASUEX010000000
VERSION     DASUEX010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9726
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1208542"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..735)
                     /locus_tag="VFV61_07160"
     CDS             complement(<1..735)
                     /locus_tag="VFV61_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX4900296.1"
                     /translation="MTNDIKELRNIAIIAHVDHGKTTLVDAMLRQSGTFRDNETVRDR
                     VMDSMDLERERGITIMAKNTSVHYQNVKINIVDTPGHADFGGEVERVLKMVDGVMLLV
                     DAAEGCLPQTRFVLRKALEARLPAIAVVNKIDRQDARPAEVVDEIYELFLDLDATNEQ
                     IEFPILYAISREGVAKKQLQDKSTDLRPLFDQIVETIPAPQPLRDDSLQLLVANIDYS
                     DYVGRLAIGRIFSGEIATGDQVVVVQH"
     gene            complement(833..1312)
                     /locus_tag="VFV61_07165"
     CDS             complement(833..1312)
                     /locus_tag="VFV61_07165"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HEX4900297.1"
                     /translation="MIRVALVEPEIPPNTGNIARLCAATCTPLHIVGVPGFRLDDRAV
                     RRAGLDYWPEVDLHRHPDVEALYRELAGARFIYLSTKGSIPYTDWVFQESDCLVFGRE
                     TRGLPESLLQANWERCLTIPMSNPRVRSLNLAVSVGIVLFEALRQTGALRHTSRQRG"
     gene            complement(1912..2640)
                     /locus_tag="VFV61_07170"
     CDS             complement(1912..2640)
                     /locus_tag="VFV61_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900298.1"
                     /translation="MMNRKRGPHHNIYGMSVVGSEWRSLPSAAARDHAEAGYTLVALL
                     ALMTLLALIAISAAPSLRQQAQREREKEAIFRGEQVADAIREYYLYRTTATGGPGEQA
                     LPTSMEQLLEGIPIPGGSKKRQVLRASAARDPLSTTGEWRTVRPRAQELLEFQRAVVV
                     YAGSILPQPTNGQIAQLQQFAVPQLTSVLDTGSARSAPGGEDSSADSSGPFVGVTSRS
                     RNNSVLYYYGIDRHDQWIFTPLFR"
     gene            complement(2627..3547)
                     /locus_tag="VFV61_07175"
     CDS             complement(2627..3547)
                     /locus_tag="VFV61_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900299.1"
                     /translation="MKLSDISNPEERKKLIWAIVLGLFAIVFLWWALFGIGPSPKPTV
                     KKANSNSNPGPGPRRVGETTTAQTVNEIKSAPLEQLRPIVFSDFVPSAPEAKRNIFVY
                     YEKPPPAPKSETGPTPTPTPPPPLLLAGIQPSNRFARTEDFTMEVTGDKFVPGVRIVV
                     DGRELTTRFISPQQLSATVPASLVANPGARSVMVKSPDGSLYSTTLSLNVSAPPTPNY
                     SYVGIIGTRRFIDTAILQDKNNREILNVQRGDVLAGRFRLTSISEKELVLVDTQLNIK
                     HTLPFSTDRDRATGPQSRPTPRVEAEDDEP"
     gene            complement(3553..4302)
                     /locus_tag="VFV61_07180"
     CDS             complement(3553..4302)
                     /locus_tag="VFV61_07180"
                     /inference="COORDINATES: protein motif:HMM:NF022201.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspMb/PilO family protein"
                     /protein_id="HEX4900300.1"
                     /translation="MNETPGKSTRPRRRTQFKANLQRLRASRSQGMFGLAEILALGVS
                     LLLLLLVVISYIYFLVPARSRLQTLQSERTRLQTQLRSSQEIIHQGLDTQSTVTRITE
                     SLDEFQSYHLSNAGQGRMGLYGELNQLIRKNGLRNTSGPAYTPLVSSTTKQSPGETRS
                     ASTKWQSVYPGIAVSLTVEGQYQNLRRFIRDLEVSKQFIIINTIELERATETNSAASL
                     DPATNTARNSLVSLRLDLATYFQMGAAEPGQ"
     gene            complement(4299..4940)
                     /locus_tag="VFV61_07185"
     CDS             complement(4299..4940)
                     /locus_tag="VFV61_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900301.1"
                     /translation="MTTKLNLASRPFSNHALPWVLTALIVAVSLISLVLILRWTGQAK
                     AQADSVQADINTLNQQEQALKAQAVAVKNSLTQEQLQTLAAAHVLVDRKRFSWSRLFA
                     DLEAALPGSVRVTRISVRDVAARGEQSLAELDLTVVSKSPATITDMIGDMDRVGIFQA
                     ELRAQNLQKGRGESGTEYELYVIYRPRAAASADSKAQDLASAEPAAAPTGGEK"
     gene            complement(5147..6055)
                     /locus_tag="VFV61_07190"
     CDS             complement(5147..6055)
                     /locus_tag="VFV61_07190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX4900302.1"
                     /translation="MERRPALVSLRGELLAVPIPLDRDEVTLGRALEADVRINDSRAS
                     RLHARIVVERNVEHNSVRCIIRDLASTNGTLINGQPLTEAVLNEGDKISIGDQLFRFD
                     MLDEIDREFQQQIHRLISHDELTGLLTSKSFFSELRREAAKAEDKSHPFCVLMMDVDH
                     FKQVNDTYGHLVGSQTLEEIGGVIKRALRAGDVAARFGGEEFAAFLLDADYAQGLVAA
                     ERVRSAVQNYEFPAARRDAPAAGTLRITISIGVASFPDDAKDPIELIELADSALYRAK
                     RGGRNRISAYRHSIAVTEGKLPPRRT"
     gene            6378..7022
                     /gene="ribA"
                     /locus_tag="VFV61_07195"
     CDS             6378..7022
                     /gene="ribA"
                     /locus_tag="VFV61_07195"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF001591.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HEX4900303.1"
                     /translation="MKRESILEEEATCTWATSDLTVERVAVAHLPTQFGEFQIAGYRS
                     LISDEEFVVLFKGEMRREVPTLVRIHSQCLTGDVFGSTKCDCGQQLQQTLRLIQKEGR
                     GAVVYQQQEGRGIGIINKIRAYSLQDAGADTVEANEQLGLAVDLREYRQCAEVLFELG
                     LCHVRVISNNPLKLQALEDAGLKIVERVSMQVEPTEKAAGYLRTKKEKLGHLLS"
     gene            7115..8215
                     /locus_tag="VFV61_07200"
     CDS             7115..8215
                     /locus_tag="VFV61_07200"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEX4900304.1"
                     /translation="MKKVPLVFGMGILLALALTPVTGLANSSAEPLVIATVIQGRSSI
                     YGNVYGEGRRPVADVYVELLDDVNSTIRQIKTDMSGRFQFSGLVDGRYMLKIRPGNTG
                     YLEYTQQVIIAAVSSVRPTEVGGRSGGSDTQHIDIALKLDERGLYGPFAAAPGVVFAQ
                     DVPAAAKQHYDEAITQLRAKKEEQAFASLRQALEVFPDYYAALDRLGGEYAVRGVDEK
                     GTNQDYLQAGLVLLTKATQVNPRSYSSMYGLGWTEYHLGMTNEAIDHLGKATTIYGKA
                     ASAYLLQAKAYRRTKMLDKAEEALKRADQLANGKDADIHWQLAGVYNDQKRFGEAADQ
                     MELYLKNAPKGEDPEKIKGLIKRLRDLQAKAK"
     gene            8300..9373
                     /locus_tag="VFV61_07205"
     CDS             8300..9373
                     /locus_tag="VFV61_07205"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEX4900305.1"
                     /translation="MKKVSSLCLGVAIGAVAASQLPVRSSSITQAFTVNPFITPQAGR
                     SVITGTVFADAHRPIADIWIELLDDFNSTISRLKTDASGRFTFGNLINGNYRLRVLPY
                     GTDYKEQVQDVTLASVSAVSGRPGADRQIIDIYLKLNDRAPVGPFAVGPRVVFVQEVP
                     PEAERLYEEGVRLLRNKKEKEGFESLKRSLEAFPTYYLALDRLGAEYAMRGNKDRSYW
                     EAARLLLEKAVEVNPRGINSVFGLGWTQYQLGLNAQAVASMERAISLNGKVPDAFLWL
                     GKAQLRVSAVDKAEVAFKRANELTKGKVPEIHKLLAKIYVDQKRYREAADELELYLKM
                     QSDTQDDAEIHELIKKMRAKAST"
BASE COUNT         2270 a         2586 c         2596 g         2274 t
ORIGIN      
        1 atgttgcact acaaccacct gatcaccagt agcaatttct ccggaaaaga ttcttccaat
       61 cgctaagcga cctacgtaat cactgtagtc aatatttgcg accagcagtt gcaatgaatc
      121 atctcgcaaa ggttgcggcg ccgggatcgt ctcgacaatt tgatcaaaca atggtctcag
      181 atcagttgat ttatcttgca actgcttttt agctacaccc tcgcgcgaga tagcgtaaag
      241 aatgggaaac tcgatctgtt cattagtggc atccagatcc aaaaacaatt catagatttc
      301 atcgacaacc tctgccggtc gcgcgtcctg tctatctatc ttattaacta cggcgatggc
      361 cggaagccgg gcttcgagtg ccttgcgcaa aacaaagcgg gtctgcggga gacagccctc
      421 ggcggcgtca actagtagca ttacgccgtc aaccatcttg agcacgcgtt ccacttcccc
      481 accaaaatca gcgtggcccg gcgtgtcgac tatgttgatc ttcacattct ggtaatgcac
      541 cgaggtgttt ttggccatga tggtgatgcc gcgttcgcgc tctaagtcca tggagtccat
      601 tacgcgatca cgcacagtct cgttgtctcg aaacgttccc gattggcgaa gcatcgcgtc
      661 gacaagtgtc gttttgccgt ggtcgacgtg agcgatgatg gcaatgttgc gcagttcttt
      721 tatgtcgtta gtcatagtgg cgttacacag agcgagcaaa cccttctagg cgaaactttg
      781 gatgttatca ctgattggtt ggtattgtcc gctgcgcgct ggtgtttgag gactagccgc
      841 gctggcgtga ggtgtgccgc agggcaccgg tttgacgtaa agcttcaaac aagacaatcc
      901 ccactgacac cgcgagatta agacttcgaa cccgcggatt tgacatcggt atcgtcaaac
      961 atcgttccca attggcctgc agcagagatt ctggtaaacc gcgcgtctca cgaccaaaga
     1021 ccagacagtc gctctcttga aacacccagt ctgtgtaagg gatgcttccc tttgtactca
     1081 gatagatgaa gcgggcgcca gctagttccc gatagagcgc ctccacatca ggatgtcgat
     1141 gaagatcaac ctcgggccag tagtccaagc ctgcacgtcg gacggcgcga tcatccagac
     1201 gaaaacctgg gacaccgaca atgtggagcg gcgtgcaggt ggccgcgcag agtcgggcga
     1261 tgttgccggt gttgggtgga atctcaggct ctaccagtgc aacgcggatc attgattgag
     1321 attagagtct atccggtacg ccgatatcaa aaacaaaagg agcgacgaac ttcactgaag
     1381 tgttcgtcgc tcctcttgct aatgccctgc aggtcgtcag ctgttggtgt gcgccaactg
     1441 aacgtgcctg cggcaacctg ctggttcgaa ctatctcttc ttggcggcct tcttggcgcc
     1501 cttctttgcg cccttctttg ttccaccctt tttcgctgct ttctttccac cttttttagt
     1561 tgccatagct tttatgtgct ccttcttttc aagtttgggg tttgcgtggt gaccacaaga
     1621 tactgtgtct gactttctct tgtgggctag tgtaaacggc gcacaacatc ttgctatata
     1681 aacgcaaatg aaccagatgt caacaactta ttactgttat tttcaaaaat aatttcggag
     1741 tgaatgcgca gttcttactc acttgaacgc acagcgagag cttactattc gtcagttgtg
     1801 ggagatcaaa atttgaacat cgcttcgcta cgcctgatca gaatttgacg cgcgcacgag
     1861 atcagaaact gctgttactt aatatcagtg atgtaccagg acctaaagcg actagcgaaa
     1921 cagcggagtg aagatccatt gatcgtggcg atcaattcca tagtaataga ggacggagtt
     1981 gtttcgactg cgactcgtta cgccaacgaa tggaccactc gaatccgctg aactatcctc
     2041 accacccggt gctgatcttg cggaacctgt gtcgagcaca ctagttagct gggggaccgc
     2101 aaactgttgt aactgggcga tttgcccgtt ggtaggttga ggcaatatgc tgcctgcata
     2161 gacaactacg gcgcgttgaa attcgagtag ctcctgcgcc cgcggtcgca cagtacgcca
     2221 ctctccggtc gttgaaagcg gatcacgtgc agcgctcgcg cgcagcacct gacgcttctt
     2281 cgatccacca ggaatgggaa tcccctcaag cagttgctcc attgaagttg gaagcgcttg
     2341 ctcaccagga ccgccggtag cggtggtgcg ataaaggtag tattcgcgaa tcgcatccgc
     2401 gacttgttca ccacgaaaga tggcttcctt ctctctttca cgctgcgctt gttgccgcaa
     2461 actcggtgcg gcggagatag cgatgagggc aagtaaagtc atcaaggcca gtaacgccac
     2521 gagggtgtaa ccggcttcag cgtggtcgcg agcagccgcc gaaggcaaag atctccactc
     2581 actgccgacg acgctcatac catatatgtt gtggtgtggt cctcgcttac ggttcatcat
     2641 cctcagcctc cacgcgaggg gttggacgcg actgaggacc tgttgctcgg tcgcgatcgg
     2701 tgctgaaggg aagtgtgtgc ttgatattta attgagtgtc gacaagcacc aattcttttt
     2761 ccgaaatgct cgtcaaacgg aacctgccgg ctagaacatc tccgcgctgc acgttcagaa
     2821 tctcacggtt gttcttgtct tgaagaattg ccgtatcaat gaagcgacgt gtgccgatga
     2881 ttccaacgta actgtaattc ggtgtcggcg gcgcactaac gttcagagac agggttgtcg
     2941 aatagagact tccatccgga gatttcacca tcacggagcg agcgcccgga ttggcaacca
     3001 gcgaagcagg caccgtcgcc gaaagctgtt gcgggctgat gaaacgcgtg gtgagctcgc
     3061 gaccatcaac cacgatcctc acaccgggca caaatttgtc accagtaact tccatcgtga
     3121 agtcctcagt tcgcgcgaac cggttggaag gctgtattcc cgccaaaagc agcggcggcg
     3181 gcggcgttgg cgtgggagtg gggcccgtct cgctttttgg cgccggtggt ggcttctcgt
     3241 aataaacgaa aatattgcgt ttcgcttccg gtgccgaggg cacgaagtcg gagaacacga
     3301 tcggccgtaa ctgctccaga ggagcgctct tgatttcgtt gacagtttgc gccgtcgtgg
     3361 tttcgccgac gcgacgcggc cccggtccgg gattggaatt agaattcgct ttttttacag
     3421 ttggcttggg actggggcca ataccgaaga gcgcccacca caaaaacacg atggcaaaca
     3481 atccaagcac aatcgcccag attagcttct tacgttcttc cggattactg atatccgaca
     3541 acttcatatc gattattgtc ccggctccgc tgcacccatc tgaaaataag tagccaagtc
     3601 cagtcgcaga ctcacgagcg agttgcgcgc cgtatttgtt gctggatcaa gcgaggccgc
     3661 actgttagtt tcagtcgcgc gctcaagttc gatcgtatta atgataataa actgcttact
     3721 gacctcaagg tctctaatga agcgtcgcag gttttgatat tgaccctcga ccgtgagact
     3781 cacggcgatt cccggataga cactctgcca cttggtgctg gcggatctag tttcacctgg
     3841 actctgtttg gtagttgaag aaacgagtgg tgtgtaggcc gggcctgatg tattgcggag
     3901 cccgttcttt cgaataagtt gatttagctc gccataaaga cccatccggc cctgccccgc
     3961 gttactaaga tggtaactct gaaattcatc cagactctca gtgattctgg ttactgtgga
     4021 ttgcgtatcc aggccctggt gaattatttc ctgagaactt cgaagctgag tttgtaatcg
     4081 ggttcgttcc gattgaagcg tctgcaggcg cgaacgagcc ggcaccagga aatagatgta
     4141 actgattaca accaacagca agagcaacag gctgacgccc agggcgagaa tttcagccaa
     4201 cccaaacatg ccctggctgc gagatgcgcg cagcctttgc aggtttgcct tgaactgagt
     4261 tcgccgtcgc ggccgcgttg atttgccggg agtctcgttc atttctcgcc acctgtcggc
     4321 gccgcagcag gttccgcgga agccaggtcc tgcgctttgc tgtcagcaga ggcagccgcg
     4381 cgaggacgat aaattacgta caattcatac tccgtcccgc tctcccctct tcccttctgc
     4441 aggttttgcg cacgcaattc agcctggaaa attcctacac gatccatgtc gccaatcatg
     4501 tccgttattg tcgccggact cttactgacc acagtcagat ccagctcggc caaactctgc
     4561 tcgccgcgcg cggcaacatc acgaacactg atccgcgtga cgcgcacact gcccggcaga
     4621 gccgcctcaa gatcggcgaa cagacgagac caggaaaacc gcttgcgatc gacaagcaca
     4681 tgcgccgcgg caagggtttg caactgttcc tgagtaagag agtttttgac ggcgaccgcc
     4741 tgcgctttca aagcctgctc ttgctggttc agagtattta tgtcggcctg cacgctatcg
     4801 gcctgggcct tggcctgccc ggtccaacga aggattagca ctagagaaat cagcgaaaca
     4861 gccacgatca gagcagtcaa aacccaaggc agggcatgat tgctaaacgg tcggctcgcc
     4921 aggttgagtt tggtagtcac tacgaaaaat ctctttggac tttgagggcc gatcgtacga
     4981 ctggccggag ttacctgagc agcatacctt ccccaagata cgtgcgcaac cgcggctctc
     5041 attaacaatt gagaagcttc ggcgccggcg gcgacttatt acacgcgtga ggaattgtct
     5101 tgtttcagaa aaatgccgac ctcaagagtg cacatgatgc tgttgcctac gtgcggcggg
     5161 gagggagttt gccttcagta actgcgatgg agtgtctgta agccgagatt cgattgcgac
     5221 cgccacgctt agcgcgataa agagctgagt cggcaagctc tatcagttca atcggatcct
     5281 tcgcgtcatc cggaaaagag gcgacgccaa tgctaatggt tatccgtaac gttccggcgg
     5341 ccggagcatc ccggcgggcg gcgggaaatt catagttctg cacggctgac ctgacgcgtt
     5401 cggcagcgac caagccttgc gcatagtctg catcgagcag aaacgcggcg aactcttctc
     5461 ctccgaagcg ggcagctaca tcgcccgcac gtagcgcccg cttaatgaca ccgccaattt
     5521 cttccaacgt ctgactacca acgagatgac cataggtgtc gttgacttgc ttgaagtgat
     5581 cgacgtccat catcaataca cagaaggggt gcgacttatc ttccgccttg gccgcttctc
     5641 ttctcaattc tgaaaagaat gacttgctgg tcagcaatcc cgtcagttcg tcgtgcgaaa
     5701 tgagacgatg aatctgctgc tgaaactctc ggtcgatctc atccagcata tcgaaacgga
     5761 ataactgatc gccaatggaa atcttatcgc cttcattcaa gaccgcctcg gtgagcggct
     5821 ggccgttgat caaggttcca ttggtggaag ccaaatctcg aatgatgcaa cgtaccgaat
     5881 tgtgctccac atttctttcg acgacgatgc gggcgtgcaa gcgcgaggcg cgagagtcgt
     5941 tgatgcgcac atctgcttca agagcgcgac caagggttac ttcgtcccga tcgagtggaa
     6001 ttggaaccgc gagcaactcg ccccggagcg aaaccagtgc gggacgacgt tccattggcg
     6061 ctgcgggagg ctgttgagca agacgcaatt gcgtctgatc ggaatcgtcg ttcctgtgat
     6121 cgtcactggt cattcagatc gagtgccgcg caggcacctg gggttccgat cgtctgttct
     6181 gaagtatcac cgatgctggg ttatcttagt ccgcccgcgc ccgagtcgca acggtattgt
     6241 gcgaagcaaa aatctgaatt caggcttatt ttggcggccc cctgcttgta gcgtccctca
     6301 cttacttcta taatcgcgtc acaaagagtt gagccggcct cagcaatcgt tcttgaaatg
     6361 aagatcagtc atttgtgatg aaaagggaaa gcattttgga agaagaagca acgtgcacat
     6421 gggcgaccag tgatctgact gttgaacgag tcgccgttgc ccatctgccg acacaattcg
     6481 gcgagttcca aatcgcgggc taccgttcgc tgatcagtga tgaagaattt gtagtgttgt
     6541 tcaaaggtga aatgcgacgc gaggtaccta cgctcgtgcg gattcactcg caatgtctca
     6601 cgggcgatgt ttttggatcg actaaatgcg attgcgggca acagctccag cagaccttgc
     6661 gcttgattca aaaagagggc cgcggggcag tggtttacca acaacaggaa ggtcgcggca
     6721 tcggcatcat taacaagatc cgcgcctact cgctccaaga cgcgggcgct gataccgtcg
     6781 aagcgaatga acagctcggg ttagctgtcg acttgcgcga atatcgccag tgtgccgaag
     6841 tgctattcga gttagggctt tgtcacgtgc gggtaatctc caacaatccc ttgaaactgc
     6901 aagccctgga agacgctggt ttgaaaattg tcgagcgagt gtcaatgcaa gtcgaaccga
     6961 ctgaaaaagc cgccggctat ttgcgcacca agaaagagaa gttaggacat ctattaagtt
     7021 agattcttga accaagtgtt tcgtccgaga ttatccccag aatctctgca ccagtaccgc
     7081 ctgtaattat ttcttattct taaggagaat aacgatgaag aaagtgcctc tagtcttcgg
     7141 catggggatc ctcttggccc tggccctgac tccagtcacc ggtcttgcca atagttcagc
     7201 tgagccgctg gttatcgcca ctgtgattca ggggcgaagc tcgatctacg ggaatgtcta
     7261 cggtgaggga cgacgtccag ttgccgatgt ctatgttgaa ttgcttgacg acgtgaattc
     7321 aacaatccgg cagattaaga ctgacatgtc gggacggttt caattctcag gacttgtcga
     7381 tggtcgctat atgctcaaaa tccgacccgg caataccggt taccttgagt acacgcagca
     7441 ggtgattatt gctgctgttt cctctgtgcg cccgaccgag gtgggcggtc gttctggggg
     7501 ctcggatacc caacacattg atatcgcgct caaattggat gaacgtgggc tctacggacc
     7561 gttcgcggcc gcaccgggtg tggtgtttgc tcaagacgtt cctgcagctg ccaagcaaca
     7621 ttacgacgaa gcaataacgc agcttcgtgc aaagaaagaa gaacaggcat ttgcgagctt
     7681 aagacaggca ctcgaagttt ttcccgatta ctatgcggct ctggaccgcc ttggcggaga
     7741 atacgcagtt cgtggtgtgg acgaaaaggg taccaaccaa gattacctgc aggccggtct
     7801 ggtcttattg accaaggcta cacaagttaa cccgcgcagc tactcaagca tgtacggttt
     7861 aggctggacc gagtatcacc tcggtatgac aaatgaagcc atcgatcacc tgggaaaagc
     7921 cacaactatc tacggcaagg ctgcgagtgc ttatctgttg caagccaagg cctaccggcg
     7981 cacaaaaatg ctggataaag ccgaggaagc attaaaacgg gcagatcaat tagcgaatgg
     8041 caaggatgcg gacatccatt ggcagttggc cggcgtctac aatgatcaga aacgcttcgg
     8101 tgaagccgcc gatcagatgg agttgtattt gaaaaacgct cccaaaggcg aagaccccga
     8161 aaaaatcaaa ggcctcatca aacgtttgcg ggatctgcag gctaaggcaa agtaacaaac
     8221 ttgcttcgca ccaggtgtac aaggccgcca cccttctaca cctgaaaggg tctcggcagt
     8281 ggcgacagga gcattcgtca tgaagaaagt ctcttcccta tgtctcgggg tggccatcgg
     8341 agccgttgca gcctcgcaac tgcccgtccg ctcaagctcc attacccaag ccttcacagt
     8401 caaccctttc ataacacccc aagcagggcg cagcgtgatc acgggcaccg tgtttgcgga
     8461 cgcacaccga ccgattgcgg acatttggat agagctgctc gatgacttca actccacgat
     8521 cagccggtta aagactgatg cctcgggacg ctttactttt ggcaacctga tcaatggtaa
     8581 ctatcgcctg cgtgtgcttc cctatgggac ggactacaag gagcaggttc aggatgtgac
     8641 cctggcaagt gtctccgccg taagcggtcg ccccggtgcg gaccgccaga ttatcgatat
     8701 ctacttaaag ctcaatgacc gagctccagt gggtccgttc gctgtcggtc cgcgggttgt
     8761 ctttgttcaa gaagtaccgc ctgaagcgga acgcctctat gaagaaggcg ttcgcttatt
     8821 gcgcaacaag aaagagaaag aagggttcga gagtctgaag aggtctctcg aagcgtttcc
     8881 tacctattat ctcgcgctcg atcgcctggg cgcggagtac gcaatgcgcg ggaacaaaga
     8941 tcgcagctac tgggaagctg cccgtctgct tctggaaaaa gcggttgagg tcaacccccg
     9001 cggtatcaac agtgtcttcg gcttaggctg gactcaatat caacttggtt tgaacgctca
     9061 agccgttgcg agtatggagc gcgccatttc acttaacggt aaggtccccg atgcttttct
     9121 gtggctgggg aaagcgcaat tgagagtatc ggcggtcgac aaggcggaag tcgcattcaa
     9181 gcgcgccaac gaattgacca agggaaaggt ccctgagatt cataagctgc ttgccaagat
     9241 ctacgtcgac caaaagcgtt accgggaagc cgcagatgaa ttggagttgt acctgaagat
     9301 gcagtcagat actcaagatg atgccgagat tcacgagctc atcaaaaaaa tgagagctaa
     9361 ggccagcaca taaatcctgc gcttcgcagt taacctaaat ctgttggggg cgtccttccg
     9421 tgggcgcccc ttttcataac gtgcggcggc cccggagtta cggactcgac ggcaaataag
     9481 acacacctgt gccgttcagc aacgtccccg gttaaaataa gcagtcataa agtaacggcg
     9541 actcaggaat gcgggcttgt ccctaccctt gtgcttaatg ccactcacct tcgcctctgc
     9601 actcaaatgt acccagtccc cttacttgcc acgactctga gggttaaatc aaagattccg
     9661 gtcccttggt accgctgtta gctttgttga cctgctggag ggcaccgtag cgctcggtta
     9721 cggctt
//
LOCUS       DASUEX010000424         3264 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_406444, whole genome shotgun sequence.
ACCESSION   DASUEX010000424 DASUEX010000000
VERSION     DASUEX010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3264
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406444"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..1389)
                     /locus_tag="VFV61_07210"
     CDS             complement(70..1389)
                     /locus_tag="VFV61_07210"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF002223.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEX4900306.1"
                     /translation="MSKIIVVIGAQWGDEGKGKIVDLLAERFDIVARYQGGHNAGHSV
                     QVGDQAFVLHLLPSGIVHKGKICVLGNGMVIDPKAFFVEADRLAQQGIDVTPARVKIS
                     SRAHLILPYHRALDHTSEERLGNEKVGTTLRGIGPAYEDKAGRRGIRTADALVPEVLR
                     SRIERNLEDANRIIMAYGGEKLDADEIFREISPLTERLGAFIGDTTHYLNVAASEGRS
                     ILLEGAQATLLDVDHGTYPFVTSSCTTVGGAIVGTGLAPNRLTGVLGIVRTYTTRVGE
                     GPFPTEMLEGEAELGHMIRERGREYGASTGRPRRCGWFDAFATRFAGEINGFSSVALT
                     KLDVLDELDEIKVCTGYKLDGRICESLPPVSQDLRRIEPIYATLPGWKSSTVGTTEMS
                     ALPVNARRYVDFLSEQMGVEIGLVSTGPERSQTIIVQDSALGRWLKD"
     gene            complement(1534..1878)
                     /gene="trxA"
                     /locus_tag="VFV61_07215"
     CDS             complement(1534..1878)
                     /gene="trxA"
                     /locus_tag="VFV61_07215"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEX4900307.1"
                     /translation="MSEHVSEVSDSSFEKDVLQSDKPVLVDFWAAWCAPCRMLAPTVE
                     AVAEKYASNARVVKLNVDDNPGTSQRYGIKGIPTLILFKGGKEEERVVGATSKEAISR
                     MIDKHVSGAATA"
     gene            complement(1935..>3264)
                     /locus_tag="VFV61_07220"
     CDS             complement(1935..>3264)
                     /locus_tag="VFV61_07220"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA];
                     GO_function: GO:0009011 - starch synthase activity
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HEX4900308.1"
                     /translation="GVDAIVVMPLYQQIDRALLEDEEYEPVAVQWRGGIRPTRVFKSV
                     AAGAPAYLIDAPEYFSRSSIYGFGDDHERFAFFSRAALALFKHLDWPPDIIHNNDWPC
                     GFAAIELRARRQYDEFYKPSRTLFSIHNIAYQGAFDPKDLWWLGFGDQPERNDFMFKN
                     AASALKAGLIASDALSTVSRRYAQEIQTPEQGYGLDWLLRARRDRFVGVTNGVDYELW
                     NPETDPHIVANFSADDLSGKLKCKLDLLRRFDLPQDPERPIIAIISRLVAQKGYDLIR
                     EIAHQITQTGAFFIALGAGAREYENFLQSWHDRYPREVGIYKGYAGEPLAHQIEAGAD
                     MFLMPSLYEPCGLNQMYSMRYGTVPIVRATGGLDDTVQNYEESSGTGNGFKFQDYRSP
                     ALLEKIREALYFYGKPEAWQQIQRNGMMMDNSWSAAAQKYIEIYKELGKL"
BASE COUNT          748 a          951 c          839 g          726 t
ORIGIN      
        1 acacgacgtt tctggattca cactcctcac cttcagcgga cgttggacat ttggacattg
       61 gaccttggac tagtccttca gccatcttcc caacgccgaa tcctgaacaa tgatcgtctg
      121 tgatcgttcc ggaccggtgg acaccagtcc gatctccacg cccatctgct cggaaagaaa
      181 gtcgacgtag cggcgcgcat ttaccggcaa agcactcatc tcggttgtgc ccacggtcga
      241 actcttccaa ccaggcaacg tcgcatagat gggttcgata cgacgcaggt cctgcgacac
      301 gggcggcagc gactcgcaaa tgcgtccatc caatttgtat cccgtgcaga ccttgatctc
      361 atccaactcg tcgagcacat ccagttttgt cagcgccacc gaactaaatc cattgatctc
      421 cccggcaaag cgcgtcgcaa aagcatcgaa ccaaccgcag cgccgcggac ggcctgtcga
      481 agcgccgtac tcgcgaccgc gttcgcgaat catgtgaccc agctcggctt cgccttcaag
      541 catttccgtg gggaaggggc cttcaccaac acgagtggtg tacgtgcgca caattcccag
      601 cactccggtc agccgattag gtgcgaggcc ggtgcctaca attgcgccgc cgaccgtggt
      661 gcacgaggaa gttacaaacg gataagtacc gtgatcgacg tcaagtagag tcgcctgcgc
      721 gccttcgaga agaatcgaac gaccttcgga agcagcgaca ttgagatagt gtgtcgtgtc
      781 accaataaaa gctccgagtc gttctgtaag cggcgaaatt tctcgaaaga tctcgtcagc
      841 gtcaagtttc tcgccgccgt atgccatgat gattcggttg gcgtcttcaa gattgcgctc
      901 aatccgcgag cgcagcactt ccggcacgag tgcatctgca gtacgaatcc cgcgccgccc
      961 tgccttgtcc tcatacgccg ggccgatgcc ccgcagcgtt gttcccactt tttcgtttcc
     1021 cagccgctct tcgctcgtgt gatccagggc gcggtgatag ggaagtatca agtgagcgcg
     1081 cgaacttatc ttgacccggg ccggtgttac atctatgcct tgttgcgcca gacgatcggc
     1141 ttccacaaag aaagccttgg gatcgatcac catgccgttg ccgagcacac agatcttccc
     1201 cttgtggacg atgcctgaag gaagtaagtg aagcacgaac gcctggtcac ctacctggac
     1261 agagtgacca gcgttgtgtc cgccctgata gcgcgccacg atatcgaagc gctcggcaag
     1321 cagatcaaca atcttgcctt tgccttcgtc tccccattgg gcgccgatga cgacgatgat
     1381 tttagacata aaaaagatgg tctttgaact taggtctttg gtctttgatc aggttcggca
     1441 gtcgatactt ttcaatcaac cactcgagct ccgtaagaga tcaaagatca aagaccaaag
     1501 ttcaaagacc aaagtttcaa agcttgttag cgattaagca gtggctgcgc cgctcacgtg
     1561 cttatcgatc atgcgtgaga tcgcttcctt cgaagtggcg cctacgacac gctcttcttc
     1621 tttgccgcct ttgaaaagaa tcaacgttgg gatgcccttg atcccgtaac gctgcgaggt
     1681 tccgggattg tcatccacat tgagcttaac gacgcgcgcg ttactcgcat atttctcagc
     1741 caccgcttcg acggtgggcg ccaacatgcg acagggcgcg caccaggcgg cccaaaagtc
     1801 gaccagcacg ggcttgtccg actgcaacac gtccttctca aagctgctat cactaacttc
     1861 acttacatgc tcactcatcg aaatctcctt gatgtcctaa actgtttttc taacgttctc
     1921 caaaatgaaa ggagctagag cttgcctaac tccttataaa tctcaatata cttctgagcg
     1981 gcggctgacc aggagttgtc catcatcatg ccgttcctct gaatctgctg ccacgcttca
     2041 ggcttaccat aaaagtaaag tgcttctcgt attttttcaa ggagcgcggg gctgcggtag
     2101 tcctgaaact tgaaaccgtt tcctgtgccg ctcgattcct catagttttg caccgtatcg
     2161 tccaaacccc cggtcgcccg cacaatcggc accgtgccgt aacgcatgct gtacatctga
     2221 ttcagcccgc acggttcata gagagatggc attaagaaca tgtcagcgcc ggcttcgatt
     2281 tgatgcgcga gcggctcgcc agcataaccc ttatagatgc ccacctcgcg cggatagcgg
     2341 tcgtgccagc tttgcaggaa gttctcatac tcgcgggcac cggctcccag ggcaatgaag
     2401 aaagctccag tctgtgtgat ctggtgtgca atctcgcgaa ttaagtcata acccttttgc
     2461 gcaaccagac gcgagatgat ggcgataatc gggcgttccg gatcctgcgg caaatcgaac
     2521 cgccgcagca gatcgagttt acatttcagt tttccggaaa gatcgtcagc cgagaagttt
     2581 gcaacgatat ggggatcagt ttccggattc cagagctcgt aatcgacccc attggtgacg
     2641 cccacaaagc ggtcgcgtcg cgctcgcagg agccaatcga gcccgtagcc ctgttccggc
     2701 gtttgaatct cttgagcata gcggcgactg acggtagaca gcgcatcgga agcgattaac
     2761 ccggctttca gcgccgacgc ggcgttcttg aacatgaagt cgttgcgctc cggctggtcg
     2821 ccaaagccaa gccaccaaag atccttcggg tcaaacgcac cctggtaggc aatgttatga
     2881 atcgagaaga gagtccgcga gggtttgtaa aactcgtcat attgccggcg tgcgcgcaac
     2941 tcgatggcgg caaagccaca cggccagtcg ttgttgtgaa tgatgtcggg gggccaatcg
     3001 agatgcttga aaagcgccaa ggctgcccgc gaaaagaacg caaagcgttc atggtcgtcg
     3061 ccaaaaccgt agatagaact ccgggaaaaa tactcaggag cgtcgattaa gtaagctgga
     3121 gctcccgcgg ctacgctctt aaaaaccctg gtaggtcgta tgccaccgcg ccattgcact
     3181 gcaacaggct cgtactcctc atcctcgagc aacgcgcgat cgatttgctg ataaagcggc
     3241 ataactacga tcgcgtcgac accg
//
LOCUS       DASUEX010000425         3411 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_515695, whole genome shotgun sequence.
ACCESSION   DASUEX010000425 DASUEX010000000
VERSION     DASUEX010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3411
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_515695"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(161..1678)
                     /locus_tag="VFV61_07225"
     CDS             complement(161..1678)
                     /locus_tag="VFV61_07225"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX4900309.1"
                     /translation="MKLHKSHGQALALCALIVALGSVVYAPSTQAQAGGATQQSTKGA
                     VVKGRAPVNKDVLRVKLPKAEEATLPNGLRVVLLRDPKVPTFSAQMVILSGGLADKSD
                     YRGLASFTAALLREGTTKRSSKDIAEQVDSLGGTLTSNSGFSSMTSTVSSSGLVENLD
                     QTFELFADVIRNPSFPAEEVEKYKARTLAQLQFQRSSAQFLAQERFSRAIYGDHPAAI
                     VAPPVESLKKLSSQDLTAFHSTYYRPNNAILAIVGDVTLKELLPKIEKAFGDWQKADV
                     PATSIPAAPAQAGSRILLIDRPGSVQTVLTLGTLGIERNSPDYFSILLADKILGGGPA
                     ARLFLNLREDKGYTYGAYSNFGGSRFRGTWASSSEVRTDVTEGAMKEFMFELKRLRND
                     KVSMEELDNAKRAIVGSFALSLEQPQSLLQNIITQKLYDLPADYWDTYPQKVAAITVD
                     DIQKVAQKYIDLEHMQIVAVGDAAKAKEVLAKYGKVEVYDADGKMVSAAGEMKNN"
     gene            complement(1691..3049)
                     /locus_tag="VFV61_07230"
     CDS             complement(1691..3049)
                     /locus_tag="VFV61_07230"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX4900310.1"
                     /translation="MRRASASFASLLVMLLAVIAVQAQVPRKVKVDFKETTLKNGLRV
                     ITVEDHSAPVVSISVNYNVGSRDERKGRTGFAHLFEHMMFQGSENIGKSEQMILVFNN
                     GGILNGTTNEDRTVYFEALPANQLELALFLEADRMRSLAITKDNLDNQRNAVQEERRL
                     GLDNQPYGKSGEIQQELLYDNFAYKHSVIGSMEDLSAANVDDVAEFFKIYYAPNNAVL
                     ALVGDFNTKDALARIAKHFESIPRQPNPPAVDMTEPEQKSERRASVDDVLARVPRVDL
                     AFKAVAGNTADFYALQVLSAALQGGQSSRLYQKLVKEKELTTGVGGFMDEKRGLGALY
                     IGANLRPGVKTDAVEAAVYEEIERLKKEPIADWELQKAKNTTRRAFVNNLQSSLSRAI
                     NLTQYAIYYNDPGLINARLDKVAAVTKDDVQRVANKYLRDTNRTVVITMPKARGAAPS
                     GQ"
BASE COUNT          739 a          940 c          911 g          821 t
ORIGIN      
        1 gaaaaccggg tgacgggtgt cggtctcaag tctcaagtag gcctcaagtc ttaagaggtc
       61 acaaatccca ggtttcaact gtctagcact ccgaatgtga cttgagacct gagacctggg
      121 acctgagacc gatccccgac gctcgacacc cgttccgctt ttagttgttc ttcatctcac
      181 ctgccgccga caccatcttg ccgtcagcat cgtaaacctc aaccttgcca tacttcgcca
      241 acacttcctt tgctttggca gcatcgccga cggctacgat ttgcatatgc tcgagatcga
      301 tgtacttctg cgcgaccttt tgaatgtcat caacagtgat ggccgcaact ttttgcgggt
      361 aggtgtccca gtagtccgcg ggcaggtcgt aaagtttctg cgtgatgatg ttttgtagca
      421 aactctgcgg ctgctcaagt gaaagcgcaa agctgccgac aatagctcgc ttggcgttgt
      481 cgagctcctc catgctcacc ttgtcgttgc gcaagcgctt gagctcaaac atgaattcct
      541 tcatcgcgcc ttcggtaaca tcggtgcgca cctcagaact cgaagcccag gtaccgcgga
      601 agcgcgagcc cccgaagttg ctataagcgc cgtaagtgta gcctttgtcc tcacgaagat
      661 tcagaaacaa ccgcgccgcc ggcccgccgc caagaatctt gtcggcaagc aaaatggaaa
      721 agtagtccgg actgttgcgt tcgattccga gcgttcctag tgtcagcaca gtctgcactg
      781 atcctggtcg atcgattagc agaattcgtg accccgcctg tgctggagct gcaggaattg
      841 aagttgccgg aacgtccgcc ttttgccaat ctccgaaagc cttttcgatt ttcggcagca
      901 gctctttcag cgtgacgtca ccgacgatcg cgagaattgc gttgttcggc cggtaataag
      961 tcgagtggaa ggccgtcaga tcctgcgacg agagtttctt gagcgactct actggcggcg
     1021 ccacgatcgc ggcgggatga tcgccataga tcgcccgact gaatctttcc tgcgccaaga
     1081 actgcgcact cgatctctga aactgcaatt gcgccagcgt tcgtgccttg tatttttcca
     1141 cttcctccgc tgggaacgac gggttgcgga tcacatccgc gaacagttcg aatgtctgat
     1201 cgagattctc gacgagacca gaagaggaaa ctgtgctcgt catacttgag aagcctgaat
     1261 tcgaggttaa ggtgccgccg agggaatcta cttgttccgc gatgtcctta ctgctgcgtt
     1321 tggtggttcc ttcacgcaaa agagcggcgg tgaaagaggc gaggccgcga tagtccgatt
     1381 tgtcagccag cccaccgctg aggattacca tctgggcgga gaaggttggc actttggggt
     1441 cgcgtaacag gactacgcgc aagccgttcg gcagcgtagc ttcctccgcc tttggcagct
     1501 tcacccggag cacatctttg ttgactggcg cgcggccctt tacgacggcg cccttggttg
     1561 attgctgagt cgcaccacct gcctgcgcct gagtggaggg cgcgtagaca acagagccaa
     1621 gggcaacaat gagggcgcac agcgcgagtg cttgtccatg tgatttgtgt agcttcattt
     1681 ctaaactcct ttactgtccg ctgggggccg cgccgcgggc cttcggcatt gtgatcacga
     1741 cagtgcgatt agtgtcgcgc aaatacttat tagcgactcg ctgcacatcg tccttggtaa
     1801 cggccgccac tttgtccagc cgcgcattga tgagaccggg atcgttgtaa tagatggcgt
     1861 actgcgtcag attaatggct cgcgagagcg agctttgcag gttgttaaca aaggcgcgac
     1921 gcgtagtgtt cttcgctttc tgcaactccc agtcagcaat cggctccttc tttagacgct
     1981 cgatctcttc gtagacggca gcttccacag catccgtctt cacgccaggc cgcaagtttg
     2041 cgccaatgta aagcgcgccg aggcctcgct tttcgtccat gaagccaccc accccggtcg
     2101 tcagctcttt ttccttcacc agcttctgat acaaacggga actctggcca ccctgtaacg
     2161 ctgccgacaa cacctgcaac gcatagaagt ccgcagtgtt tccggcgacc gccttgaatg
     2221 ccagatcgac ccgcggcaca cgagccaaaa catcgtcaac cgaggcacgc cgttcgctct
     2281 tctgctcagg ctcagtcata tcaactgcgg gcggattcgg ctggcgcgga atgctctcga
     2341 agtgcttggc aattctggcg agtgcgtcct tggtattaaa gtcaccgaca agggcgagca
     2401 ccgcattgtt tggtgcgtag tagatcttaa aaaactcggc aacatcgtcg acgttagctg
     2461 ctgaaagatc ttccatcgaa ccaatcactg agtgcttgta ggcaaagttg tcgtagagta
     2521 attcctgttg aatctcaccg ctcttgccgt agggttgatt gtcgaggcca agtcgccgct
     2581 cctcctgcac agcatttcgt tggttgtcga gattgtcttt ggtgatcgcc aggcttcgca
     2641 tccgatctgc ttcgagaaac agcgcgagct cgagttgatt tgccggcaag gcttcgaaat
     2701 aaacggtgcg gtcctcgtta gtggtgccat tcaggatgcc accattattg aacaccagga
     2761 tcatctgctc agacttgccg atattctccg agccctggaa catcatgtgc tcgaagagat
     2821 gcgcaaaccc ggtgcgtccc tttcgttcgt cgcgcgatcc gacgttgtag tttacggaaa
     2881 tggaaactac cggagcgctg tgatcttcaa cggtgataac gcgcaggccg ttctttagag
     2941 tagtttcttt gaaatcaact ttgactttgc gtggcacctg cgcttgcacg gcaatgacgg
     3001 caagcaacat aaccagcaac gacgcaaacg atgcggaggc acgtctcata tagctaactc
     3061 cttccagttt gatgaagcaa aagtaatgaa cgattccaat gtgagttact tagcagagca
     3121 aacgatcagt gacaatactc ccgaatgctc tagcggagct gtttctctaa cagtcagggg
     3181 agacgaattg tagttgagtt ttgcaacgcg cttgcgagcg gtcgcgaaac gattctcgct
     3241 gtggagcgtg aagcgacttt aagtcgctgc cgagatgagt gtcaagcgac gcccaaaggt
     3301 cgacgtacga gggcgatcat cgtaaaggac tgattggatt tcatgaattg ccgattcgcg
     3361 attaccaagt gccgatttca gacgaaaccc cgacgtctta ttttattgag g
//
LOCUS       DASUEX010000426         3469 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_997598, whole genome shotgun sequence.
ACCESSION   DASUEX010000426 DASUEX010000000
VERSION     DASUEX010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3469
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_997598"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            189..1037
                     /locus_tag="VFV61_07235"
     CDS             189..1037
                     /locus_tag="VFV61_07235"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: protein motif:HMM:TIGR00571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HEX4900311.1"
                     /translation="MQSASSARLTKNSPANSDRALPKSPLKPPLKWAGGKRWLVPFIS
                     AYWQKHSSRRLVEPFCGGLSVALNLQPEEALLNDVNPHLINFYEQVKRGLQLTIAAKY
                     DSELYYRHRERFNQLIRTNGVDAEAAQLFYYLNRTCFNGLCRFNQSGEFNVPFGSYSR
                     NLKYAPDFNEYQALFRKWTFSNTDFEKLRLRPDDFVYADPPYDVEFTTYSQGGFSWSD
                     QERAASWLAQHPGPVILSNQKTKKIVELYRDLDYKLKFRKAPRRISCTGDRTEALEVL
                     ALRNLE"
     gene            complement(1081..2781)
                     /locus_tag="VFV61_07240"
     CDS             complement(1081..2781)
                     /locus_tag="VFV61_07240"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX4900312.1"
                     /translation="MKIILAVLSVLLLNFGWLAPQQRPAAQVADLVFINGAVYTANDR
                     QPHAEAVAIKGDRILFVGSNTAARSHVGKNTRVIDLQGRAVLPGLTDSHHHLSGVGFR
                     EMNLNLEGTTSLQDFLAKVKARVDQAKPGEWITGRGWIETFWQPPVFPTRQDLDGVAP
                     NNPVILGRADGHGAVVNSAALKLAGIDKTTANPFGGEISKDANGEPNGMLLDAAQGLV
                     RSHIPGTTAADAERAVILGVNRNISLGWTQVQDAGGSYPEVELFKKLYNEGKIKLRIY
                     KAIHGPSGSSARLLSEGPIIGAFNHRFTLRTIKVVSDGALGSRGAALLAPYADKPDTK
                     GFLTVKAEELAPMLTQALKVGVQVETHAIGDYANRFTLDEYEKAFKAVPAAERKVADP
                     RWRIEHSQIVNPTDIPRFAKLGVIPSMQPSHAIGDLHFAPSRLGIERLAGAYAWQSFI
                     KSGVIVPGGSDAPVERGEPLIEFYAAVARKDQKGFTGAGWHPEEAVTRDQALKMFTIW
                     PAYAAFEEKLRGTIEVGKLADLSIFSADIMKIPEPEILKARCVMTVINGEVVFSLFDN
                     "
     gene            3104..>3469
                     /locus_tag="VFV61_07245"
     CDS             3104..>3469
                     /locus_tag="VFV61_07245"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX4900313.1"
                     /translation="MKTEILKLSTSRGATTAHVARPDHETSTAIILIQEWWGINDHIR
                     DIAGRYAAEGFVCVAPDLYRGKLAKTTAEASALMHDLAIADGIETISAALAETKTKYN
                     AGRFGITGYCMGGTFALRAA"
BASE COUNT          787 a          960 c          921 g          801 t
ORIGIN      
        1 tttgattccg ctgaaattga gtcaagtata ttaattgccg tttaacggtt tccgcccttc
       61 gtggcgttta aagcacctgg ctgaagctag gtgtgaatga gaccttccgg ggtctgtggc
      121 taggcagcgc tcgcgcaaga cagttaatcg tgtacatcga cccagcctta tggtattcaa
      181 cgttttgaat gcaaagtgct tcttcagcgc ggcttactaa aaactcgccc gcgaattcag
      241 atcgcgcgct ccccaagtcg ccgctcaaac caccactaaa atgggccggt ggcaaacgct
      301 ggctggtacc cttcatcagc gcttattggc aaaaacatag ttctcgacgg ttggtagaac
      361 ccttctgtgg cggactttcg gttgcactca atcttcaacc cgaggaagct ttacttaacg
      421 atgtcaatcc tcatctgatc aatttctacg agcaggtcaa acgagggctg caactaacaa
      481 tcgcagcaaa gtatgactcc gagctttact accggcatcg tgaacggttc aatcagctga
      541 tcaggactaa tggcgttgac gccgaagcgg cgcagctctt ctactatctt aatcgtactt
      601 gcttcaacgg gctttgtcgt tttaaccaga gcggagaatt caatgtgccc ttcggctcgt
      661 attcccgcaa cttaaaatac gccccggact ttaatgagta tcaagcgtta tttcgtaagt
      721 ggacatttag taacacggac tttgaaaagc tgcgactgcg gccagatgat ttcgtttatg
      781 ccgatccgcc ttacgatgtt gagttcacca cctatagcca ggggggattt tcgtggagcg
      841 atcaggaacg agccgcaagc tggctagcgc aacatcccgg tccggtaatt ctgtcaaatc
      901 aaaaaaccaa aaagattgtc gaactctatc gggatcttga ttacaaattg aagtttcgca
      961 aagcgccgcg tcgaatcagt tgcaccggcg atcgcaccga agcgctggag gtactggcgc
     1021 tgcggaacct ggagtagcga agtcccaagt ctcaggagac ttgagatttg agactctttg
     1081 tcagttatca aacaaactaa acaccacctc gccattaatc accgtcatca cgcaacgcgc
     1141 ctttaagatc tccggctccg gaatcttcat gatgtccgcc gagaagatcg acaagtccgc
     1201 gagcttccct acttcaatcg tgccgcgcag cttttcttca aacgccgcat acgccggcca
     1261 gattgtaaac atcttcaaag cctgatcacg cgtgactgct tcttccgggt gccagccggc
     1321 gccagtaaat cccttctggt ccttgcgcgc caccgcagcg tagaactcaa tcaagggctc
     1381 accgcgttcc accggggcat cggaaccgcc gggaacgatc actcctgact taataaaact
     1441 ttgccaggca tatgcgcctg ccaggcgctc aattcccaga cggctcggtg cgaaatgcag
     1501 gtctccgatc gcatgcgaag gttgcatgga aggaattaca ccaagcttgg cgaagcgcgg
     1561 tatgtccgtt ggattcacaa tctgggaatg ctcgatgcgc caacgcggat cggcaacttt
     1621 ccgttcggcg gctggcaccg ctttgaatgc cttttcgtac tcgtcaagcg tgaagcgatt
     1681 tgcgtaatca ccgatcgcgt gggtttccac ctgcacaccc actttcaatg cctgggttaa
     1741 cattggcgcg agctcttccg ctttcacggt taagaagccc ttcgtgtccg gcttatcagc
     1801 atagggtgcg agcaaggcgg ctccacgtga tccgagagcg ccatcggaaa ctactttgat
     1861 ggtgcgtaag gtgaagcggt gattgaaggc gccaatgatt ggaccttcag aaagtaagcg
     1921 cgcggacgac cccgaaggac cgtgaatcgc tttgtaaatc cgcagcttga tctttccttc
     1981 gttatacagt tttttgaaaa gttcgacttc cggataactg ccgcccgcgt cctgcacctg
     2041 cgtccaaccc agactgatgt tgcgattgac gcccagaatg actgcccgtt cagcgtcggc
     2101 ggcagtcgtt cccgggatat gactgcggac caggccctga gcagcgtcaa gcagcattcc
     2161 gttcggttcg ccgttcgcgt cttttgagat ttcgccgccg aaaggatttg ccgtggtctt
     2221 atcaatcccc gcgagcttca atgcagcgct attaaccaca gcgccatgac catcggcgcg
     2281 gccgaggatg acgggattgt tgggcgccac accgtcgaga tcctgtcgcg tcggaaacac
     2341 cggaggttgc caaaaggttt cgatccagcc gcgaccggtt atccattcac cgggttttgc
     2401 ctgatcaact cgggctttta ccttggcgag aaaatcctgg aggctggttg tgccttccag
     2461 attgagattc atctcccgaa agccaactcc cgagaggtga tggtgcgagt ctgtaaggcc
     2521 gggaagcact gcccggccct gaagatcgat cacgcgcgta tttttgccta cgtgacttcg
     2581 cgcggccgtg ttactgccga caaagaggat gcggtcgcct ttgatcgcga ctgcctcggc
     2641 atggggttgg cggtcattgg cggtatagac ggcgccgttg atgaagacga gatcggcaac
     2701 ctgtgccgca ggacgctgtt gtggagccag ccaaccgaag ttgagcagca gcacactcaa
     2761 cacggccagg ataattttca tggtttcttc cttctgtgga acttaggagt gcaggcgaac
     2821 catcgatgtc ttatatcacg caggcagcca gtttcgggaa cgattgcgac aagcctgcga
     2881 agcaggcgaa agcgtaaagc ccagggcgta agccctgggt aatcgacgca acgaggaaca
     2941 agcccgcaaa gcgggcgaca gatgattggc gagacctgcc gcccgttcca cgggctcatg
     3001 atgttcatac gtccgatcac cctggggctt acgccccagg ctttacgctt ttgcctgctt
     3061 cgcaggctaa aatgcaaaca ttgatattgg aggttagtta tttatgaaaa ctgagattct
     3121 aaagctttcc acttcacgcg gcgctacgac tgcacatgtt gcgcggcctg accacgaaac
     3181 cagcaccgca attatcctga ttcaggagtg gtggggtata aacgatcaca ttcgcgatat
     3241 cgccggtcgc tatgcggccg agggttttgt ctgtgttgcg cccgatcttt atcgaggcaa
     3301 actcgccaag accaccgccg aggcgtcggc gctcatgcat gacctcgcaa tcgcagatgg
     3361 catcgaaacg attagcgcgg ccctcgcgga aacgaagacc aagtataacg ctggccgctt
     3421 tggcatcacc ggttactgca tgggcggaac tttcgctctg cgcgcggcg
//
LOCUS       DASUEX010000427         3684 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1117535, whole genome shotgun sequence.
ACCESSION   DASUEX010000427 DASUEX010000000
VERSION     DASUEX010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3684
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1117535"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..671
                     /locus_tag="VFV61_07250"
     CDS             <1..671
                     /locus_tag="VFV61_07250"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX4900314.1"
                     /translation="GSFGAITLLSKSRTSYTTEHVRLLESVCQHASSALNNALTFEKT
                     KESALVDALTELPNARAFYMLLGQRIAECQRMNHESLSVICMDIDNFKGINDEYGHAI
                     GDRVLASIAGVIRKELRQMDVLARYAGDEFVIIMPMASQQMAVSVRDRIRTAVEGQKF
                     SVRTGKTVELGLSLGIACFPADGETTEELLTAAASTMQRDKHRRKTMLTVANSPINTI
                     GALS"
     gene            888..2021
                     /gene="pdhA"
                     /locus_tag="VFV61_07255"
     CDS             888..2021
                     /gene="pdhA"
                     /locus_tag="VFV61_07255"
                     /inference="COORDINATES: protein motif:HMM:TIGR03182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HEX4900315.1"
                     /translation="MSKQSQPSNSRAAANEQNSVAVIDADAPDEGQMRDDREAVKQEQ
                     KRHLAAYDHKTLVELLRQMLLIRRFEEKSAEAYSLGKIGGFCHLYIGQEAVAVGAISA
                     IRKDDYVLTSYRDHGQAIAKGMSPDAVMAELFGKAGGCSQGKGGSMHMFDKSIGFLGG
                     HAIVGGQIPLATGAAFATKYLGTDQVTLCFFGEAAVNQGAFHESLNIAQLWKLPCIYI
                     CENNQYGMGTSLQRAMSLHDISQKACAYEMAAEFVDGMDVLAVREATARAVERARKDY
                     LPTLIEVRTYRFMGHSMSDPGNYRTRAEIEKYQERDPLKVFVATLLEEKIISEKDYQA
                     MEKRVQEEVAHALRFADESPLPETGELYTDVYANPITPGKTIK"
     gene            2046..3038
                     /locus_tag="VFV61_07260"
     CDS             2046..3038
                     /locus_tag="VFV61_07260"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF008854.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HEX4900316.1"
                     /translation="MESMAVITMREALNQALREEMLRDENVFLMGEEVAEYQGAYKVT
                     KGLLQELGPKRVIDTPITELGFAGLGVGAAMVGLRPIVEFMTFNFSILATDQIINSAA
                     KMRYMSGGQFKIPIVFRGPGGSAYQVSSQHSQALESWFAYFPGLKVVMPSTPADAKGL
                     LKSAIRDDDPVIFIEQERMYGNKGEVPEDAEFIVPLGVADIKREGTDATIVARSLMVP
                     IALKAAEVLAGEGVSCEVIDPRTIRPLDIDTIVASVKKTNRVVVAEESHPFCGVAAEI
                     SSEIMERAFDYLDAPVKRLSGADVPMPYAKNLEALAIPTVDQLVAAVREVAYLA"
     gene            3213..>3684
                     /locus_tag="VFV61_07265"
     CDS             3213..>3684
                     /locus_tag="VFV61_07265"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEX4900317.1"
                     /translation="MPKLSPTMEEGQLSRWLKKEGDKVSMGEPLAEIDTDKATMEMQA
                     LANGVLRKIIIKEGESAPLGQLIAVIGEPDEDISALLVGISAAPKAEAAASETKPEQA
                     PAPETLPAKPANAAAKAADPGGNGATPAQTTATSGRLIVSPLAARMAAEAGVDLR"
BASE COUNT          892 a          955 c         1051 g          786 t
ORIGIN      
        1 atggttcatt cggcgccatt actctgctgt caaagagccg aacgtcctat acgaccgaac
       61 atgtacgcct gctggagtca gtttgtcagc atgcttcaag cgcgctcaac aatgcgttga
      121 cttttgagaa gaccaaagaa agcgcgctcg tcgatgcgct cacagagctg cctaacgctc
      181 gcgccttcta catgctgctg ggccaacgaa ttgccgaatg ccagcgcatg aaccacgaat
      241 cactttccgt gatctgcatg gacatcgaca atttcaaggg catcaacgac gagtatgggc
      301 atgcaattgg cgatcgagtt ttggcgagta ttgccggcgt gattaggaag gagctcaggc
      361 agatggacgt gctggcgcgt tatgccggag atgaattcgt gatcatcatg ccgatggcgt
      421 cccaacaaat ggcagtctcc gttcgtgatc ggatccgcac cgcggtggag ggccagaagt
      481 tttcggtccg cactggtaag acagttgagc tgggtttgag cctggggatc gcctgcttcc
      541 cagctgatgg cgagactacc gaggagttgc tcaccgcggc ggccagcacc atgcaacgag
      601 acaagcaccg ccgaaagacg atgctgactg tggccaactc tccaatcaac actattggcg
      661 cgctcagtta aggacggcgc tgcctcaagt agagcgctca tcgcagatcg cggtgggcgc
      721 ttttttcatg ttgcagagac agtatcctga tgcagtgcaa ggaccttttt gcagggcttg
      781 cgataagtcg cgtataatcc cctccactac aaggaacatc gccgggttga ttaggatgtc
      841 accacatcgc cacccgcgtg tcggaagaag acaaagaaga ctgaaacatg agtaaacagt
      901 cccagccctc gaacagccgt gcagcggcca acgaacaaaa tagtgttgcc gtcatcgatg
      961 cggacgcgcc cgacgaaggc caaatgcgcg acgatcggga agcggtgaag caggagcaaa
     1021 agcgacacct cgctgcctac gatcacaaga ctttggttga actactgcgg caaatgctgc
     1081 tgatccgtcg cttcgaagag aagtcggccg aagcctacag cctgggaaag attggaggct
     1141 tctgtcatct ctacataggc caggaagccg tggccgtggg cgctatctcg gcgattcgaa
     1201 aggacgacta tgttctcacg agctatcgcg atcacggaca ggcgatagcc aaaggaatgt
     1261 cgcctgacgc agtgatggca gaactgtttg ggaaggcggg tggttgttcg caaggcaagg
     1321 gcggctcgat gcatatgttt gataagagca tcggctttct tggtggtcat gcgattgtcg
     1381 gtggtcagat accactggcc accggtgccg ctttcgctac caagtatctg ggcactgatc
     1441 aagttacgct ttgcttcttt ggcgaagctg cagtaaatca gggcgccttt cacgaatcgc
     1501 tgaatatagc gcagctctgg aaactgccct gcatttatat ctgcgagaac aaccagtatg
     1561 gaatgggcac gtcattgcaa cgcgcaatgt cgctgcacga catttcccag aaagcctgcg
     1621 cttatgagat ggcagccgaa tttgttgatg gcatggatgt cctggcagta agagaagcga
     1681 cggcgcgagc ggtggaacgt gcgcgaaaag attatctgcc aaccttgatc gaggtgcgaa
     1741 catatcgctt catgggacac tcgatgtcag atccgggtaa ttaccgtacc cgtgcggaga
     1801 tcgaaaaata ccaggagcgc gatccactga aagttttcgt agcgactctg cttgaagaga
     1861 agatcatcag tgagaaggat taccaggcaa tggaaaaacg ggtccaggag gaggtcgctc
     1921 atgcgcttcg tttcgctgat gagagtcccc tccccgagac aggtgaactt tatacagatg
     1981 tctatgccaa tccgataact ccaggcaaga ccataaagta gcgcgttcaa aggcgaattg
     2041 aggaaatcga atcaatggct gtgatcacaa tgcgcgaagc tctcaaccag gcgctacgcg
     2101 aagagatgct ccgcgatgaa aatgtgtttc tgatgggcga ggaagttgcc gagtatcagg
     2161 gcgcatacaa agtcacaaag ggcttgctcc aggaattagg tcccaaacgc gtgatcgata
     2221 cgccgatcac tgagctgggc ttcgccggac tgggcgtcgg cgctgcgatg gttggcttgc
     2281 gcccaatcgt ggagtttatg actttcaact tctcgattct ggcaaccgat cagattatta
     2341 attcggcagc taaaatgcgg tacatgtccg gtggacagtt caagatcccg attgtatttc
     2401 gcggccccgg gggctccgct tatcaagtct cgtcgcagca ctctcaggct ttggaatcgt
     2461 ggtttgccta ctttccagga ctcaaggttg tcatgccttc gacgcccgcc gatgcaaagg
     2521 gtctgttgaa gtccgcgatt cgcgatgacg atcccgtgat cttcatcgag caggagcgaa
     2581 tgtacggaaa caaaggcgag gttcctgagg atgctgagtt catcgtgccc ctcggggtcg
     2641 ccgatataaa acgtgagggc accgacgcca ctatcgtcgc tcgctcacta atggttccga
     2701 tagcactaaa ggcagcggaa gtcctggccg gcgagggtgt ctcctgcgag gtgatcgatc
     2761 cgcgcactat tcgaccgctg gacatcgaca cgattgtggc ttcggtgaaa aagaccaatc
     2821 gtgtggttgt tgctgaagag tcgcacccgt tctgtggcgt ggcggcggag atcagcagcg
     2881 aaatcatgga gcgcgctttc gattatctcg atgctccggt aaagagactt tcgggcgcag
     2941 atgtgcctat gccttacgca aagaatctcg aagcactcgc cattcctacg gtcgaccagc
     3001 tggtggcagc tgttcgtgag gtggcttatt tggcgtgaat tcgtgaatag tgaattcgtg
     3061 aatcgtaaga ggcgaccggt aacaaccgat tggtgagcaa cgattcacaa gtctctattt
     3121 caatttacga tcgccaactg gcgttcacga ttcacaattt cacgatttca cgatttccga
     3181 ttcacgaggc ttgaatggca actcaggtga tcatgccaaa gctatcgccc accatggagg
     3241 agggacagct ttcgcgctgg ctcaagaaag agggcgacaa ggtttcaatg ggcgaacccc
     3301 tcgccgaaat tgacactgat aaggccacaa tggaaatgca ggcccttgcg aacggcgtac
     3361 tgcgcaagat catcatcaag gaaggcgaat ccgctcccct ggggcaacta atcgccgtca
     3421 ttggtgagcc tgacgaagac atctcggcat tgttggtggg catcagtgcg gcgcctaaag
     3481 cagaagctgc cgcatctgaa actaaaccag aacaagcccc agctcccgaa acccttcccg
     3541 ctaaaccagc gaatgccgcc gcaaaagcag cagatcctgg aggcaacggt gccacaccgg
     3601 cccagacgac agcgacttcc ggccgtttga tcgtgtcacc actagcagca cgaatggcgg
     3661 ctgaggctgg tgtcgacttg cgct
//
LOCUS       DASUEX010000428         5611 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_515729, whole genome shotgun sequence.
ACCESSION   DASUEX010000428 DASUEX010000000
VERSION     DASUEX010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5611
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_515729"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..885)
                     /gene="trpB"
                     /locus_tag="VFV61_07270"
     CDS             complement(<1..885)
                     /gene="trpB"
                     /locus_tag="VFV61_07270"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEX4900318.1"
                     /translation="MERALPDKSGYWGRYGGRYVPETLMAPLEELTAAFKRATADPDF
                     HRELDGLLADYSGRPTPLFHAARLTAEAGGARIYLKREDLSHTGSHKINNTLGQALLA
                     RRMGKRRVIAETGAGQHGVAAATVCALFDLECVIYMGTADMQRQALNVFRMRLLGAEV
                     RDVNTGSRTLKDAINEALRDWVTNVADTYYLLGSALGPHPYPVMVRDFQSVIGRETRK
                     QTLEIEGRLPDILIACVGGGSNSIGLFHPFLDDESVRMIGVEAGGCGNDLGQHAARFN
                     LDSGGRPGVLQGTMSYVLQ"
     gene            complement(912..2345)
                     /gene="trpC"
                     /locus_tag="VFV61_07275"
     CDS             complement(912..2345)
                     /gene="trpC"
                     /locus_tag="VFV61_07275"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF006945.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEX4900319.1"
                     /translation="MNSDFLSAITQRKRMRLDRARSEHPLEILHARAKEVREKTRPHR
                     LQRTLASDGDAKVIAEIKRASPSKGMIKEVLDPAQLARAYGRGGAAAISVLTEEEYFR
                     GSLDDLRAVCEIGALPVLRKDFIIDEYQIFETAAAGADALLLIVAALDDDQLLRFRLI
                     TEDELGMDALVEVHTSSEMFRAKRCGATLIGINNRDLRTFAVSLKTSEELARIAPAEA
                     ILVSESGLGSGHDLKRLREVGFSGFLIGEALMRAKDPAVALQTLISDASEESLVRVKV
                     CGITNLKDAQMCVRAGVDMLGFNFYRASSRYIAPQETRRIIDQLPAAVTSVGIFVNEE
                     SPEKVAGIAELAGVAAVQLHGEETPSYCQALENWFVIKALRVREDFVAEQVSEYETNA
                     ILLDAFSSKAPGGTGDRFDWSVARQALPFVSKLFLAGGLTPENVAPAAAFVRPYAVDV
                     CSSLESAPGKKDEERVHAFVAAVRSIA"
     gene            complement(2342..3439)
                     /gene="trpD"
                     /locus_tag="VFV61_07280"
     CDS             complement(2342..3439)
                     /gene="trpD"
                     /locus_tag="VFV61_07280"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEX4900320.1"
                     /translation="MRNERQTSDKTSPWLTPMSGQTEVTEPDLLRQLLPRLIRGENLR
                     SDEAANLLYELLGNVATDVQVAAALVALAIKTETVGELAGMATAMRARAVRLQCRHKY
                     FIDTAGTGSSRVKTFNVSTAAAFVVAGAGLPVAKHGARAATSRTGSADVLSALGVGVS
                     AAPGVSQRCLNEIGICFMFAPLYHGATARVAGIRRELGIHTTFNLLGPLTNPAGAPNQ
                     IIGVWHPRLVESLARALAKLGTNKSWVVHGFDGLDEITLADKTIVAEVSPGDIRMFEI
                     APEDFGLRRASLESVRCDDAESSACTIREVIAGTRRDEARNLVVINAAAGLFIGGQAG
                     DLIEARRLAEESIDGGAAHAKLDQLVKATNQ"
     gene            complement(3436..3999)
                     /locus_tag="VFV61_07285"
     CDS             complement(3436..3999)
                     /locus_tag="VFV61_07285"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEX4900321.1"
                     /translation="MIIVIDNYDSFTYNLVQYLGELGEDIAVRRNDQVTMNEIEALCP
                     DRILISPGPGTPKDAGISLELISFFAGHVPILGVCLGHQAIAQAFGGYVGRAPEIVHG
                     KASEICHDGRTIFKGLEHRFRAGRYHSLIVERESLPNCLEVSATTTEGLIMGLRHRDL
                     KVEGVQFHPESILTTQGKRLLANFLEL"
     gene            complement(3996..>5611)
                     /locus_tag="VFV61_07290"
     CDS             complement(3996..>5611)
                     /locus_tag="VFV61_07290"
                     /inference="COORDINATES: protein motif:HMM:NF012640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HEX4900322.1"
                     /translation="CWRRVLFLGVTKVGTSRKEQNLGNSIFPMSDIKPSSFEEFELEA
                     ERGNVVPVVRSVPTNSQTPIGALIHITSQARYAFLLESVEGGERTARYSFLGAEPEMI
                     VRGRGAQTIIESAGASKTRNVPLPEFVREYFNSRRLARRPDLAPLAGGAVGYFAYAAA
                     RWFDPAFTSRAEEVSPDDAILMFYRTVLAFDRLRRRIDITCVVFTEEAGGSRTRLRAL
                     YERAVGETAHIEQLLEGSAGSRSETGNTQSRIPECSSFTSNWTRDGFQDAVLRVKEHI
                     AAGDCYQVVLSQRFSKSVTVPPLTVYQALRAINPSPYLYFLRLGEESIVGSSPEMLVR
                     CRGPRLDYRPIAGTRQRGATRAEDWLLTSEMRSDEKEVAEHMMLVDLGRNDLGRVADY
                     GSVQVGDLMNVECYSHVQHLVSHLHARLRSDLDRFDAFGSCFPAGTVTGAPKVRAMEI
                     ISQLEPDARGIYSGAMLYADYSQNLDSCIAIRTIVMRNGTASVQAGAGIVADSVPESE
                     YEETINKAKALIRAIEIAENKTGRNRLPT"
BASE COUNT         1221 a         1644 c         1482 g         1264 t
ORIGIN      
        1 ttggaggacg taactcatcg tgccctgcaa gacgccggga cgcccgccgc tgtccagatt
       61 gaaacgagct gcatgttgtc ccaggtcatt gccacatcct ccggcttcca cgccgatcat
      121 tcgcaccgat tcgtcgtcaa gaaatgggtg aaatagacca atagagtttg agccaccccc
      181 gacacacgca atcagaatgt ctggcagtcg tccctctatt tcgagagtct gctttcgcgt
      241 ctcacgtccg atcacgcttt ggaaatctcg caccataact ggataagggt gcggccctag
      301 tgcgcttccc agcaggtaat atgtgtccgc cacatttgtt acccagtcgc gcaacgcttc
      361 gttgatggcg tccttaagtg tgcgcgagcc tgtgtttaca tcacgcactt ctgcgcccag
      421 gagccgcatg cgaaagacat tgagtgcttg ccgctgcatg tcagcggtgc ccatgtagat
      481 tacgcattcg agatcaaaca gagcacagac agtcgccgct gccactccat gctgcccggc
      541 gccggtctcc gcgatcacgc gacgcttgcc catgcgccgc gccaaaaggg cctgaccaag
      601 ggtgttgttt attttgtgtg agcccgtatg tgacaggtct tcgcgcttca gataaatacg
      661 cgctccgcct gcttctgcgg tcagtcgggc agcatgaaaa agtggcgtag gacgccccga
      721 gtagtcagcg agaagcccat ccaactcacg gtgaaaatcg gggtctgcgg tcgctctttt
      781 gaaagctgcc gttaactctt cgagcggcgc cattagagtc tcgggtacat agcgtccgcc
      841 gtagcgtccc caatacccgc ttttatctgg tagtgccctt tccatattcg ttgtcctgaa
      901 ccgagtaacg ttcaagcaat actcctgacg gcggcaacaa aagcgtgaac tcgctcttcg
      961 tcttttttac cgggcgcgct ttccaacgag ctgcacacat caaccgcgta ggggcgaaca
     1021 aatgcggccg ctggtgctac gttctccggc gtcagaccgc ctgcgaggaa tagcttcgaa
     1081 acgaaaggca gcgcttgtct ggcgacagac caatcgaagc ggtcgccagt tcctccgggc
     1141 gcttttgaac taaatgcgtc gagtagtatg gcgtttgttt catactcaga tacctgttca
     1201 gcgacgaagt cttcgcggac ccggagggcc ttgatcacaa accagttctc taacgcctgg
     1261 cagtaggagg gggtctcttc gccatgcaac tgaaccgctg caacgccggc cagttcggca
     1321 atgcccgcga ctttttctgg actctcttca ttaacgaaga tgccgacgct tgtcacggct
     1381 gcgggcaact gatcgatgat gcgacgcgtc tcttgcggcg cgatatagcg cgaactggct
     1441 cggtaaaaat tgaagcccag catgtcaacg cctgccctga cacacatctg cgcgtccttg
     1501 agattagtga tgccgcagac cttgacgcga accaacgact cttcgctcgc atcgctaata
     1561 agcgtctgca aagcaacagc aggatctttc gctcgcatta acgcttcacc gatcaagaag
     1621 ccgctgaacc caacctctct caagcgctta agatcatgtc ccgaacccaa gccgctctcg
     1681 ctgacgagaa tcgcctctgc aggtgctata cgcgccagct cttctgaagt cttgagtgag
     1741 actgcaaagg tccgcaggtc tcggttattt atcccaatga gcgttgcacc gcagcgcttg
     1801 gcgcggaaca tttcgctgga ggtatgcact tccaccaggg cgtccatccc gagttcgtct
     1861 tctgtgatca ggcggaagcg taaaagttga tcgtcgtcga gagcggcgac gattagtaat
     1921 aaggcgtccg ccccggctgc agccgtttca aagatttggt actcatcaat aatgaagtcc
     1981 ttacgcaaga ctggaagggc tccgatttcg cacacagcgc gtaagtcgtc cagcgatccg
     2041 cgaaagtatt cttcttcggt taatacggag atcgccgccg ccccacctcg cccgtaagcg
     2101 cgcgctagct gggccggatc cagtacctcc ttgatcattc ccttggatgg tgaggcgcgt
     2161 ttgatttcgg cgatgacttt agcgtcgccg tctgatgcaa gcgtacgctg cagtcggtgt
     2221 ggccgagtct tttcccgcac ttccttggcg cgcgcgtgca ggatttcgag cggatgctct
     2281 gaccgtgcgc ggtcaagacg catccgtttg cgctgcgtaa ttgctgagag gaaatcggag
     2341 ttcattggtt tgttgctttc actaactgat ccagcttggc gtgcgccgca ccaccatcaa
     2401 tactttcttc cgcgagacgc cgggcctcga ttaaatcgcc agcctggccg ccgataaata
     2461 gtcccgcagc agcattgatc acaaccaggt ttcgcgcttc atcgcggcgt gtaccggcga
     2521 taacttcacg aatcgtacag gcgctgctct cggcgtcatc acagcgtacc gattcaagcg
     2581 aagcgcgtct cagcccaaag tcttcaggcg caatttcaaa cattctgata tcccccggtg
     2641 aaacctcagc cacgatggtc ttatcggcga gtgtgatttc gtccaggcca tcaaacccgt
     2701 gcaccaccca tgatttattc gttcccagct tcgcgagcgc acgcgccagg gactcgacca
     2761 gacgtgggtg ccaaactcct atgatctggt taggggcacc tgccgggttc gttaagggac
     2821 cgagaagatt aaatgttgtg tgaataccaa gctcgcgcct gattcccgcg acccgtgctg
     2881 tagctccgtg ataaagcggc gcgaacataa agcagatacc gatctcgttc aggcatcgct
     2941 gcgaaactcc tggcgctgcg gaaactccaa caccaagcgc cgaaagaaca tcggcgctgc
     3001 ctgttctact ggtagccgcc cgcgccccat gttttgctac aggcagacca gcgcctgcaa
     3061 caacgaaagc ggcagccgtc gagacgttga aagtcttgac ccgactcgag ccggtgccgg
     3121 cagtgtcgat gaaatactta tgacgacatt gaagacgcac agcccgcgcg cgcatcgccg
     3181 tcgccatgcc ggcgagttcc cccacggtct cggttttaat tgccaatgcg accagtgccg
     3241 ccgcgacctg gacatcggtg gccacattac ccagcaactc atagagaaga ttcgcagctt
     3301 catcgctgcg caagttctca cctcggatta gccgaggtaa cagctgtcgc agaagatccg
     3361 gctctgtaac ctccgtctgc cctgacatgg gtgttagcca tggcgaagtc ttgtcgcttg
     3421 tttgcctctc gttcctcata gttcaagaaa gttagcgagc agcctttttc cttgcgtggt
     3481 taggattgat tccgggtgaa attgcacgcc ttcaaccttc aaatcgcggt ggcgcagccc
     3541 catgatcaat ccttccgttg tagttgcaga gacttcgaga caattaggca aagactcgcg
     3601 ttcgacgatg agcgaatgat agcggcctgc cctgaagcgg tgttcgaggc ctttaaaaat
     3661 ggttctgcca tcgtgacaga tctccgaggc tttcccatga acgatctccg gggcgcgccc
     3721 gacatagcct ccaaaggcct gcgcgattgc ctgatggccc aggcatactc cgaggatcgg
     3781 aacatgtccg gcaaaaaaag aaatcaactc aagtgatatt cctgcatcct taggagtacc
     3841 cggccctggc gagatcagaa tcctgtcggg gcatagcgct tctatctcgt tcatcgttac
     3901 ctggtcgtta cgacgcacag cgatatcctc tccaagctcg ccaaggtatt gaacgaggtt
     3961 gtaagtaaag gaatcgtagt tgtcgatcac gatgatcatg taggtaatct gtttcgcccg
     4021 gttttgtttt cggcgatttc gatggcgcga atcagcgctt tagccttgtt gattgtctct
     4081 tcatattcag actcgggtac tgagtcagcc acgatgcctg ctcctgcttg caccgaggcg
     4141 gttccgttcc tcattacaat cgtgcggatg gcgatgcacg agtccaggtt ttgggaatag
     4201 tcggcgtaga gcattgctcc tgaatagatt ccgcgcgcgt ctggttcgag ctggctaata
     4261 atctccatcg cgcgcacctt cggcgcccca gttaccgtcc ccgccggaaa acacgagccg
     4321 aaagcatcga accgatcaag atcgctgcgc agtcgggcat gtaaatgact gaccagatgt
     4381 tgcacatggg agtaacactc gacgttcatt aagtctccca cttggaccga accatagtcg
     4441 gcaactcgac caagatcatt gcgcccgagg tcgacgagca tcatgtgctc tgctacctcc
     4501 ttctcatcgc tgcgcatttc tgaggttaag agccagtctt cagccctggt cgcgccccgc
     4561 tgcctggtcc cggcaatcgg gcgatagtcc agacgtggtc cacgacagcg cacgagcatt
     4621 tcgggggatg agccgacgat cgactcttca cctaaccgca gaaaatataa gtatggcgat
     4681 gggttgatgg cgcggagcgc ctgatagaca gtgaggggcg gaacagtcac gctcttgctg
     4741 aagcgttgtg aaagcacgac ctggtaacaa tcacctgcgg caatgtgttc cttgacacgc
     4801 aggacagcgt cttgaaagcc gtctcgggtc cagttcgatg taaaggaaga acactctgga
     4861 atcctggatt gcgtgttacc ggtctctgag cgtgatccgg cacttccctc tagcagctgc
     4921 tcaatatgcg cagtttctcc gactgcgcgc tcgtaaaggg cccgcagacg tgtgcgactc
     4981 ccaccagctt cctccgtgaa taccacgcac gtaatgtcga tccgccggcg gagtctgtcg
     5041 aaagcaagca cagttctgta gaacatcaaa atcgcgtcat cgggagatac ctcttcagct
     5101 ctggatgtga aggcaggatc gaaccagcgc gccgctgcgt aggcgaaata accaacagca
     5161 cctcctgcca ggggagctag atccgggcgc cgcgcgagcc gtctgctgtt gaaatactct
     5221 cgaacaaact ctggaagagg aacatttcgc gttttgcttg cacctgctga ttcaattatc
     5281 gtttgcgcgc cccggccgcg aacgatcatt tccggctcag cgcccagaaa ggagtacctt
     5341 gccgttcgtt cgcccccttc cacagattca agcaggaagg cataccgtgc ctgcgaagtg
     5401 atatgaataa gcgcacctat cggcgtttgc gagttagtag gtacggatct aacgaccgga
     5461 accacatttc cacgctccgc ttcgagttcg aactcctcaa atgaactggg tttaatgtcg
     5521 ctcataggaa aaatagagtt ccccaggttc tgttcttttc gcgaggtgcc aaccttagtc
     5581 acgcccaaaa acaaaacccg ccgccaacaa g
//
LOCUS       DASUEX010000429         3593 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_883110, whole genome shotgun sequence.
ACCESSION   DASUEX010000429 DASUEX010000000
VERSION     DASUEX010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3593
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_883110"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..998)
                     /locus_tag="VFV61_07295"
     CDS             complement(123..998)
                     /locus_tag="VFV61_07295"
                     /inference="COORDINATES: protein motif:HMM:NF025007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin-like domain-containing protein"
                     /protein_id="HEX4900323.1"
                     /translation="MNQPVPLVDRVHQPTPREFYYRYVRPNRPVVLTGITDNWKALRN
                     WNPEHFRTHYPTAKVVYTAWESKEPTNDPTSYYRNRKRRTTTLGAFIDAMNSDQQTSQ
                     DYVSQYPIFNELPQLENEIDSLEPYMNIPRLYPAALQSKVRKAATFWLGPAGIVTPIH
                     FDSAHNLLVQIYGRKKLLLIPPQQSRLLYYPSLHLGHVNYSPVDVEAPDFERFPRFKK
                     AKPLELELAPGEVLFIPVRWWHYARALERTISLNFWWYSVDSLRRMWHPYLTYKMDRL
                     MRNLRVRKAGLPPLF"
     gene            1286..1642
                     /locus_tag="VFV61_07300"
     CDS             1286..1642
                     /locus_tag="VFV61_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900324.1"
                     /translation="MAKCKPFSVTLAVLAPSNKRQVSFGITRGCIGDTPFYLIIFVLR
                     DKVEGGFQDRVKLDIRVGDTLNPKAEALMTQGLNMAQLEFLSGPITDRAKALKKGTTE
                     DAKLADLNQQLLEIKK"
     gene            1639..>3593
                     /locus_tag="VFV61_07305"
     CDS             1639..>3593
                     /locus_tag="VFV61_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900325.1"
                     /translation="MISMQTILLPALHGERRRTLVPCARFAAAVVALLFVCGFSGVAV
                     RGQTETREGKTCALSSDHYERAGYTVKAIEIETPIDFVGVVRKPLEAKLAASEAALDA
                     QHKGLKVDERFSNLAYTELLADLTSRIGTLEEGERFKLVATVPSLESCDEAKKTLKVV
                     YWVVTSGPISYLSGIFEKRHDRVTREFAPGAFAKLAGKFLPQPFAGYNRSRAIFAGTD
                     AAYRFTNDLINKIDLSASGSGSSATGDFALAGDRNFKRGFLEHAEWRLGYRYSNIPGD
                     AVRLKDATVLGQLFAASRPLGSQDLILRFGASIESGNRQSDLDQAALTTRTLANSGYA
                     GAKMYIGGTLNLGRQSWTGTYALQFGANSRAIRVDYVKQIVDTSYSVRFLPREHKPLR
                     IDAQFNAGHISSGANAIPLAERFFGGNATREFIQGDDWQIRSTPFIRSIPQNRLNRVG
                     PGLPIGGEGFFSANLTLAQTIWAKPAIPSEILRDVRVKQALGGQLAGSRLFAVASYLG
                     EAPQFGELKQTLKTDTDSILKEIQTDLHAVNPDAVGIDLEEFGAEVESVRDRIAEREK
                     DEDTGEVKSSPLEFAQIRSLAVGFNGKPETSRIGALIQTINDDILVPLQESGAIEAHA
                     RLKADKERLEAARQTIQKRYEEISNLAT"
BASE COUNT          862 a          960 c          963 g          808 t
ORIGIN      
        1 ggcttcgacg aaactagcat tcgagcgcgc ttcggttcgc tccaggcgcc gagatagaga
       61 aaggtcagaa aagatttgcg ggttgttatc agtcatttat caacttgggc aatgctcacc
      121 acctaaaaca gcggcggcaa gccagccttc ctgaccctga gattcctcat cagccgatcc
      181 atcttgtaag tcaaatacgg atgccacatt cgccgcagac tatccactga ataccaccag
      241 aaatttagcg agatagtgcg ctcgagtgcg cgcgcataat gccaccagcg cactgggatg
      301 aacagcacct cgcccggcgc taactcaagt tccagcggct tggctttctt aaatcgcgga
      361 aagcgctcga agtctggcgc ctcaacgtcc acaggactgt agttcacgtg accaagatgc
      421 aaactgggat aatagagcag tcgcgattgt tgcggcggaa tcagcagcag tttttttcgt
      481 ccatagatct ggacgagcag attgtgcgct gaatcaaagt gaatgggcgt aacaatcccc
      541 gccgggccaa gccaaaacgt tgccgccttt ctaactttgc tttgcaatgc ggctggatag
      601 agccgcggaa tattcatgta gggctcaagc gagtcaatct cgttttctaa ttgcggcagt
      661 tcgttgaaaa tcggatattg cgaaacatag tcttgcgaag tctgctgatc agagttcata
      721 gcatcgatga acgcgcctaa ggtcgtagtg cgacgctttc gattgcggta ataactagtt
      781 ggatcgttag taggctcttt gctttcccag gccgtgtaaa ccacttttgc agttgggtaa
      841 tgggttcgga agtgttcggg attccaattt ctgagagctt tccaattgtc cgtaatgccc
      901 gtgagcacaa ccggtcgatt aggccggacg tagcgataat aaaactcgcg cggcgttggc
      961 tgatgaaccc ggtcgacgag tgggacaggt tggttcatac actaattacg ggcctgcgac
     1021 ttaacatggg tacagcccga acctatggcg caattttggg tggggcgaca ccgccaccat
     1081 ctgacagcaa agccaaactg attccagcta aggatctcgt cctgggcctg ttcagcaaac
     1141 agctagaatg acaaagccaa ttctcttgta tcgtatctca ttcaagatta gtatgagctg
     1201 tcccgaacag aagcagtgat ctcacaacga tttcaggaat ttcttacctc gctggcatgt
     1261 aaccaagcac gaaaggaacc ctcaaatggc aaagtgtaaa cccttctcgg taactctcgc
     1321 cgtcctcgca cccagcaaca agcgccaggt atcttttggt atcacccgcg gctgcattgg
     1381 cgatactccg ttctacctga tcatctttgt cctgcgcgat aaagtcgaag gtggttttca
     1441 ggatcgcgta aagctcgaca tccgcgtggg cgataccctc aacccaaagg ccgaggcatt
     1501 gatgacgcaa ggtttgaaca tggcccagct tgaattcctg tctggcccga ttaccgatcg
     1561 cgccaaggcc ctgaagaagg gaaccacgga agacgccaaa ttggctgact tgaatcagca
     1621 gttgctggaa atcaagaagt gatcagcatg cagacgatat tgctgccggc gcttcacggg
     1681 gagcgccgcc gcactcttgt gccgtgcgcg cgcttcgccg ccgcagtggt ggcgttgcta
     1741 ttcgtttgcg gcttctcagg cgtggcggtc cggggccaaa ctgagaccag ggaagggaag
     1801 acctgtgccc ttagttctga tcactatgaa cgggcaggct atacagtcaa agcgatagaa
     1861 atcgaaacac caatcgactt tgttggcgtc gtccgcaaac cactcgaagc aaagctcgcg
     1921 gcctcagaag ctgcactgga cgcgcagcac aaaggcctca aagtcgacga aagattttct
     1981 aacctcgcct ataccgaatt gctggccgac ctcacgagcc gtatcggcac gctcgaggag
     2041 ggcgaacggt tcaagctggt cgccacggtt ccctcactgg aatcgtgtga tgaagcaaag
     2101 aaaactctga aggttgtcta ctgggtggtg actagtggtc cgatctcata tctctcaggc
     2161 atctttgaaa agcgacacga tcgtgtaacg cgcgaattcg ctccgggagc ctttgcaaaa
     2221 ctcgcgggaa aattcttgcc gcaacctttc gctggctaca accgctcgcg cgcaatcttc
     2281 gcgggcacag acgcggccta tcgattcact aacgacctca tcaataaaat cgacttgagc
     2341 gcatcaggtt caggttcgag cgcaactggt gacttcgctt tggctggcga caggaatttt
     2401 aagcgtggct tcctggaaca cgcggagtgg cgccttggtt atcgctactc caatattccc
     2461 ggcgatgcag tgcgactcaa ggacgcgacg gtcctggggc aattgttcgc tgcctctcgg
     2521 ccgcttggtt ctcaggatct catcctgcgg tttggtgctt ccattgaaag cggcaatcgg
     2581 caatctgatt tggatcaagc cgccctgacg acccggacgt tggccaactc cggttacgct
     2641 ggcgcgaaaa tgtacatcgg cgggacgctg aatctgggaa gacaatcctg gaccggaact
     2701 tacgcgttgc aattcggtgc gaacagccgt gctattcgcg ttgattacgt taagcagatt
     2761 gtcgatacct cctattcggt tcgttttctg ccgcgcgaac ataagccgct gcgaattgat
     2821 gcccagttca acgccgggca tatcagctcc ggcgcaaacg caatccccct cgccgagcgt
     2881 ttctttggtg gcaatgcgac gcgcgagttt atccagggcg acgattggca aattcgcagt
     2941 actccgttca ttcgcagcat tccccaaaat cgtttgaatc gagtcggccc tggtttgcca
     3001 atcggcggcg aagggttttt ctcagcaaac ttaaccttgg cgcagactat ctgggccaaa
     3061 ccggcaattc ccagcgagat acttcgcgat gttcgagtga agcaggcgtt gggtggacag
     3121 ttggccgggt cccggctgtt cgcggtcgcc agttacttag gcgaggcgcc gcagtttggc
     3181 gagctcaaac aaacattgaa gactgacacg gacagtatcc tcaaagaaat tcaaaccgac
     3241 cttcacgctg ttaatcctga tgccgtcggg atcgacctgg aagagtttgg cgctgaagtc
     3301 gaaagtgttc gcgacagaat tgctgaacgc gaaaaagacg aagacactgg tgaagtaaag
     3361 tcctcgcctc tcgagtttgc tcaaatcaga agtcttgccg tcggctttaa cgggaaacct
     3421 gaaacctcga gaatcggcgc cctcattcaa acaatcaacg acgatattct ggtcccgctg
     3481 caagagtcgg gagcgatcga agctcacgct cgcttaaagg ccgacaagga acggcttgag
     3541 gctgcgcggc agacgatcca aaagcgatat gaagagattt cgaatctcgc aac
//
LOCUS       DASUEX010000430         5566 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1659867, whole genome shotgun sequence.
ACCESSION   DASUEX010000430 DASUEX010000000
VERSION     DASUEX010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5566
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1659867"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..930)
                     /locus_tag="VFV61_07310"
     CDS             complement(<1..930)
                     /locus_tag="VFV61_07310"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEX4900326.1"
                     /translation="MRIAIFGTGGAGGYFGARLADAGEDVVFVARGEHLQAIRKQGLR
                     LETPAGNIIARGEATDDPTQIGVVDLVLVGVKTWQVKDAARALRPLIGAETLVVPLQN
                     GVEAAGELTAELGSAHVLGGLCGTISRVVCPGHILSIGETNFIKFGELDNSRSERAER
                     LRQTFERAGVKADVPSDIQAAVWEKFIFVAPYGGIGAVTRAPAGVIRSLPETRSMLEL
                     GMREILELSRALRVSLSEDIVEKSLRLIDSLAPGATTSLQRDIADGKPSELEAWNGAV
                     VRLGRAANVPTPLHEFIYHSLLPSELQARGEASF"
     gene            complement(1035..1307)
                     /locus_tag="VFV61_07315"
     CDS             complement(1035..1307)
                     /locus_tag="VFV61_07315"
                     /inference="COORDINATES: protein motif:HMM:NF023174.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3303 family protein"
                     /protein_id="HEX4900327.1"
                     /translation="MLYLIIEHFKDNDPVPVYRRFRDSGRLAPEGLHYVSSWVDDKLE
                     RCFQLMETEDRSKLDQWILNWNDLVEFEVYPVISSQEAAERVSRLF"
     gene            1466..2371
                     /locus_tag="VFV61_07320"
     CDS             1466..2371
                     /locus_tag="VFV61_07320"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX4900328.1"
                     /translation="MPASESILLFFIDGLGIGARGPSNPLDGLAAAEPLAIFPDEAPS
                     ILGGTLVSTDPRLGIAGRPQSASGQTTILTGVNAPALLGYHKQGFPNAAMREIIAEHS
                     IFLQLKNAGIEPATFANAYSQRFFDERPRWVSATTAAVEAAELQFRNIKDLKEDRAVY
                     QDFTNHFIIERGEDVETRTPTRAAEVLASIVAAHRFTLYEYFITDKLGHKQDFEGARR
                     VLQQLAAFIRALLERVDLRTTSVILTSDHGNIEDLSSRNHTLNDVPTIVWGANRDGIA
                     GRIKDLADITPAIVDSLTKKATVHP"
     gene            2368..3777
                     /locus_tag="VFV61_07325"
     CDS             2368..3777
                     /locus_tag="VFV61_07325"
                     /inference="COORDINATES: protein motif:HMM:NF023128.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4900329.1"
                     /translation="MTSASPADSLAPVATHHPEKNNPREIFGWKIYDWANSAFSTTIA
                     GALYGPYLTEITQKAVGENGVVFNLGTIGAITAKSFFPTCVSVAVFLQIFVLPILGSV
                     ADYSNLKKKLMAVFCYIGVTATCLMFFVTGNLYWLGGLLFIIANLSFGAAVVLYNGFL
                     NEITTEDQRDKVSSRGFAYGYLGGGFLLALNFLLVLNAGRLGLDTALAVRISLLSAGL
                     WWGGFALITFSRLKSRAAEKTLPPGKSYLTVGFSELGRTFRELARLKSTLRYLIGYLF
                     FNDGIQTVISMASVFLSQELFIAKGIPTDQSFLLAIFLLVQFVAIFGALIFEKLAYAI
                     GTKRAVLVSLIIWSGVVIYGYGFLQNTRDAWVMAAIIGIVMGGSQALSRSLFSRMIPE
                     GREASFFGLYEVSERGTSWLGPLIFGIVVAMTDSYRQALLSLIGLFLIGTIILYLTDT
                     DRAIHEAGNLLPEEAARSH"
     gene            complement(4323..4601)
                     /locus_tag="VFV61_07330"
     CDS             complement(4323..4601)
                     /locus_tag="VFV61_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900330.1"
                     /translation="MANRKGVFIGAYVPNHLKESLRRRAAAEHRTLSQEITRILEEAV
                     HGRGLPAGSIDRRNKETTPRRRSSDPTPRRRAVDTDYAPTGSKSGDDR"
     gene            complement(4675..5206)
                     /gene="rnpB"
                     /locus_tag="VFV61_07335"
     ncRNA           complement(4675..5206)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VFV61_07335"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(5287..>5566)
                     /locus_tag="VFV61_07340"
     CDS             complement(5287..>5566)
                     /locus_tag="VFV61_07340"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEX4900331.1"
                     /translation="LQGFINRRAQIAWGPGFVSIFIVAFLFGAFHLPNPALMAATFVG
                     GLVWATVYQRAPNLLALAISHGLMTWILISTLPSNLLHGLRVGYKFIG"
BASE COUNT         1208 a         1580 c         1467 g         1311 t
ORIGIN      
        1 aaaactggcc tcgccgcgtg cttgcagctc cgacggcaga aggctgtgat agataaactc
       61 atggagcggt gtgggtacgt tcgccgctcg acccagacga acgactgctc cgttccaggc
      121 ctccagttcc gaaggcttgc cgtcagcaat atctctttgc agcgaagttg ttgcgcctgg
      181 ggccagcgag tcaataagtc gcagcgactt ctcaactatg tcttccgaca gcgaaactcg
      241 aagcgcgcgg gacagctcca ggatttcccg catccccagc tccaacatgc tccgcgtttc
      301 gggtaaggaa cgtatcacac cagcaggggc acgggtcacc gcaccgattc ctccgtaagg
      361 ggcgacgaag ataaacttct cccacacggc tgcttgaatg tcggaaggga cgtctgcctt
      421 tacgccggcg cgttcgaacg tttgtcggag acgctcggct cgctcgctgc ggctgttgtc
      481 cagctccccg aacttgatga aattggtctc gcctatactc agaatatgtc ccggacagac
      541 gacccgactg attgtcccgc aaagaccgcc cagaacgtga gctgagccga gttcggcggt
      601 taactcgccc gccgcttcga cgccattttg caaaggcacg actagcgttt ccgcgccaat
      661 cagcgggcgc agcgcacgcg ccgcatcctt tacctgccag gtcttgacac caaccaggac
      721 caagtccacc acgcctatct gcgtcggatc gtcagtcgcc tctccgcgag cgatgatatt
      781 gcccgcgggt gtttcaagtc gtagtccctg tttgcgaatc gcttgtaagt gttccccccg
      841 cgccacgaaa actacgtcct ctccggcgtc agccagacgc gcgccaaagt aaccacccgc
      901 tccgcctgtg ccgaagatcg caatgcgcat gaaggtctct gctggacggc gaacgatcgt
      961 ctaaggaact cccgacctcg gttcctctcg gtttagtggc gctcaatagc tcggcaagtc
     1021 tctcccggct gcggtcagaa aagtcgtgaa actcgttctg ccgcctcttg agaagaaatg
     1081 accgggtaaa cttcgaactc gaccagatca ttccaattga gaatccattg gtcgagtttg
     1141 gagcggtcct cggtttccat cagttgaaag caacgttcga gtttgtcatc cacccagctt
     1201 gatacgtagt gcaatccctc cggcgcgagt cgaccgctgt ccctgaagcg gcgatagacg
     1261 ggcacaggat cattatcctt aaagtgctca atgattaagt agagcaattt ccctctccca
     1321 agaacctgct gagttcgctg aagcttccaa ttgcgggcgc ggattctact actgcaccgg
     1381 tcatcaaagg tagtgccccg gatcagaatt caccgtccaa gccttacttg cacgtaatca
     1441 gcggcgatgg tattcatcgt gatgaatgcc agcctctgaa tcaatccttc ttttctttat
     1501 cgacggcctg ggaattgggg cgcgcgggcc gagcaatccg cttgacggtc ttgctgctgc
     1561 agagccgctc gctattttcc ccgacgaagc accaagtata ctcggcggca cactcgtctc
     1621 tactgacccc cgactgggaa ttgccggccg gccccagagc gcgtccggac aaaccacaat
     1681 tctgaccggc gttaatgctc cagccttgct cggctatcac aagcagggat ttcccaatgc
     1741 cgcgatgcgc gaaatcattg ccgagcattc gatctttctc caactgaaga atgctggcat
     1801 cgagccggcg actttcgcca acgcctattc gcaaaggttc tttgacgaaa gaccgcgctg
     1861 ggtgtcggcg actacggcag ccgtcgaagc tgcggaactg cagtttcgta atatcaagga
     1921 tctcaaagag gatcgcgctg tctatcagga cttcaccaac catttcatca ttgaacgtgg
     1981 tgaggatgtt gagacgcgaa cgccaactcg cgcagctgaa gtgttggcga gcatagttgc
     2041 cgcgcatcgc ttcactctct acgagtattt cattactgac aagctggggc acaagcagga
     2101 cttcgaaggc gcgcgcagag ttttacagca gcttgcggcc ttcatacgag cgttgctgga
     2161 gcgcgttgat ttgcgaacta cttccgttat actcaccagc gaccacggga acattgaaga
     2221 cttatcttcg cgcaaccata cgttgaatga tgttcctaca atcgtttggg gcgccaaccg
     2281 cgacggcatt gctggtcgga tcaaagatct tgcagacatc acacccgcga tcgtcgatag
     2341 tttgacgaag aaagcgacag tacatccatg acctcagcct cccctgctga ctccctggcg
     2401 ccagtcgcaa cccatcatcc ggaaaagaat aacccgcgcg agatttttgg ttggaagatc
     2461 tacgactggg ctaactcggc tttctctaca accatcgccg gcgcactcta cggaccttat
     2521 ctgacagaga taactcagaa ggcggtgggc gagaacggag ttgtcttcaa tcttggcacg
     2581 atcggcgcga ttactgccaa atcattcttc ccaacctgtg tttccgttgc cgtgttcctg
     2641 caaatctttg tgttgcccat ccttggctcg gtggctgact attcgaatct caaaaaaaag
     2701 ctaatggccg tcttttgcta catcggcgtg acagcgacct gtctgatgtt ctttgtcaca
     2761 ggaaatcttt actggttggg agggctgctt tttattatcg ccaacctctc atttggtgcc
     2821 gcggtggttc tgtataacgg ctttctgaat gagatcacca ccgaggatca gcgagacaaa
     2881 gtttccagcc gcggttttgc ttacggctat ctcggcggcg ggtttttact ggcgctcaat
     2941 tttttgctgg tcctcaatgc gggcaggcta ggcctggaca cagccctcgc ggtgcgcatt
     3001 tcgctactgt cggccggact ttggtggggt ggtttcgcgc tgatcacttt ttcacgactg
     3061 aaatcacgcg ctgcggagaa aactcttccg cccggcaaga gttatttaac agtaggcttc
     3121 agcgagctgg ggcgaacgtt tcgggaactg gcgcgactca aatcaacctt gaggtaccta
     3181 atcggctact tgttctttaa cgatggcatt cagacggtca tcagcatggc ttctgtattt
     3241 ctgtcgcagg aactgttcat cgccaaggga atacccactg accaatcctt tctgttagcc
     3301 atctttttgc tggtgcagtt tgtggcaatc ttcggtgcgc tgatttttga aaagttggcc
     3361 tacgccatcg gcaccaagcg cgccgtgctt gtttctctaa tcatctggtc tggcgttgtg
     3421 atttacggct acggttttct acaaaatacc cgcgatgcct gggtaatggc ggcgataatt
     3481 ggaatcgtta tgggcggttc gcaagcgctc tcgcggtccc tcttctcgcg catgataccg
     3541 gagggacgtg aggcatcgtt ctttggactc tacgaggttt cagagcgggg cacctcctgg
     3601 ctgggcccgc tgatcttcgg aattgtagta gcgatgacgg actcttatcg ccaggcgtta
     3661 ctgtcactta ttggtttgtt tctgattggc actattattc tttacctgac agacaccgac
     3721 cgggcgattc atgaggcggg caatttgtta ccagaggagg ccgcgaggtc gcattgagca
     3781 gacagtccgc ggtagcttgc gaatgctggg cgacaccgta cggtacagga gcgatagcga
     3841 caataacgat tgccgattac taattggaaa ttgccaaccg gcctttggcg aagggattca
     3901 atcgaaaatc ggcaattgga aattggcaat cgaatcggtc gctcccccct cccgtactgg
     3961 accgacttcg ctaccgatat taattaacct gccagcttcc tttgctttcc cgtcgccgat
     4021 ttttccaggc actcgcggga acaatactct gacaaatttg aaccgggacc gattgcccga
     4081 tcggctggta tccaggtgcc acacgcaaca cagcgcacca gtctattttc agttttaact
     4141 gagccactac gccgcctggt tgaatctgcg gaatcgagag tgccgcgcag tatgcctaag
     4201 actttcttca caatcgcaat gtagggctcc agacgcgaat agatgaagat gaacagcaat
     4261 gcgagaacgg caccaatgac gagatatttc agaatggtct ttgcactccc cgtccggaca
     4321 acttaacggt cgtcaccaga tttactacca gtgggggcgt aatctgtgtc caccgcgcgc
     4381 cgccgcggcg tcggatctga agagcgtcgc cgtggtgtgg tttccttgtt acggcgatcg
     4441 atgctgcccg caggtagacc tcgaccgtga acggcttctt ccaggattct ggtgatttcc
     4501 tgagagagag ttcggtgttc ggcggccgcg cgtcgtcgta gagactcttt cagatgattg
     4561 ggtacgtatg ctccgataaa gactcccttg cgatttgcca tgctgacggc tccttcagaa
     4621 gatagtggat cgtgaagttc agatagtgag cacctaagag cttgctgtgg ccatgcaccg
     4681 gacaggtctg taagccgaat tctgtctcca ctctcagaac tgagatctca gatctgaaat
     4741 ttgagatctc agatccaaga gcggagtagc gatcattcat ctagtccagc cgttgccaac
     4801 tggatcgagc gacctacccg gaggtcgttc ccgatagaat cgggattggg cgggcagccc
     4861 tggttgacgc ctccctattt ggtcttgcac tgcgaggagt ttgcctggcc gcgcgtgtca
     4921 ccacgcgcgc cggtgcgctc ttacattaag ccccgccagg ggccgcaccg tttcacccat
     4981 caccccgctc tcaaactgac aagtcagtct agagatcggg gctggtctgt tctctgttgc
     5041 acttgtcgtc gtctgcgaat cccagatctc aaatctgaaa tttcaaacct gagagctgag
     5101 atcctcaaac gccccgctgt tagcgggctc gctgcccttc agtgttcgga ctttcctctc
     5161 ccgcagcacc attaggtgcc gcagcagcga ccgcccgacc tgtccgtgca tggcattggc
     5221 atcatagcaa atcgtgttga tacgcgtaca cccacgagcc aaactgttta attaccttgg
     5281 gggatatcac ccgatgaact tatagccgac ccgcagcccg tgaagcagat tgctgggaag
     5341 cgttgagatg aggatccagg tcatcagccc gtgtgaaatt gccagcgcca gaaggttggg
     5401 agcccgctga tacacagttg cccagacgag gccgccaaca aaagtagcag ccatcaatgc
     5461 cgggttaggc aaatgaaacg ctccaaaaag aaaggcaaca ataaagatac tcacaaaacc
     5521 agggccccag gcaatttggg cccgacggtt aatgaacccc tgtaag
//
LOCUS       DASUEX010000431         6308 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_883259, whole genome shotgun sequence.
ACCESSION   DASUEX010000431 DASUEX010000000
VERSION     DASUEX010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6308
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_883259"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..920
                     /gene="truB"
                     /locus_tag="VFV61_07345"
     CDS             <1..920
                     /gene="truB"
                     /locus_tag="VFV61_07345"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEX4900332.1"
                     /translation="IDKPAGMTSHDVVQRVRRLLGERRIGHTGTLDPFATGVLVLLIG
                     RATRLAQFLSGADKEYEATIRLGFATDTGDREGNPISEKLKTQTTWSDDEIRNALQRL
                     RGEILQMPPMYSAKKRAGQRLYKLARKGEQIERMPVPVTIHNLEPLIANAGLLKSNDD
                     GTSDVQVRVVCSAGTYVRTLAEDFGKLLGVGAHLTELRRTRAGDFLLRDAATLEQLQQ
                     RTEEDSLRTILLPPDAALSSMPFLYLSAEDVQRVRHGMALPRPAEIWREGEKVRMKDE
                     HDELIAIGVYDSGNRLLRPRVVLAQENFD"
     gene            1197..3134
                     /locus_tag="VFV61_07350"
     CDS             1197..3134
                     /locus_tag="VFV61_07350"
                     /inference="COORDINATES: protein motif:HMM:NF019902.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrkA family serine protein kinase"
                     /protein_id="HEX4900333.1"
                     /translation="MEEQDPNKQFNISERLEDLRREHEALRWEGTFRDYFELVTQNPR
                     IAQLSHARINDMIHAAGIERLNEGTHHELISYNFFSSELFGIEEPIARLVEYFKSAAQ
                     RLEVRKRILLLMGPVGGGKSTIVSMLKRGLEDWTNTDEGAVYAIKDCPMHEEPLHLVP
                     HQLRPEIEKHYGIYIEGDLCPQCRYSLEHSYGGRHEDVKVHRVLFSEKERVGIGTFSP
                     SDPKSQDITELTGSIDLSTIGEIGVESDPRAYRFDGELNIANRGLMEFVEMLKVDEKF
                     LYSLLTLSQEQSIKTGRFAMIYADEVILSHSNENEYIAFAGNRKSEALQDRIILVRVP
                     YNLRVSQEERIYDKLLHQSEVLRNVHLAPNTLRVAAMFAVMTRLEEPKRQNVDVAKKM
                     KLYDGEDVEGYKSKDVRELKEETVREGMDGISPRYIINRLSGALVRDGVTCINPIDAL
                     RAIKDGFEQHTGISADQRERYLNMISLARKEYDELAKIEVQRAFVYSFEEMARTMCNN
                     YLDNVEAYCNKERIKDPITEEEMDPDEQLMRSIEEQIGISDNAKNTFRQEILIRISSY
                     ARKGKPFEYNSHERLKEAIEKKIFADLKDVVKITTSAKTPDPDQLRKINEVVDRLVRD
                     HGYCPVCANELLTYVGTLLSR"
     gene            3207..4184
                     /locus_tag="VFV61_07355"
     CDS             3207..4184
                     /locus_tag="VFV61_07355"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX4900334.1"
                     /translation="MKKRYLFAGLSGVVAAAFATKLLIRPRDVNWEESRDVVFHAEHS
                     RFVAVDGVRIHYQEAGERDAPAMILIHGFASSTLVWSKVLLELAAEGFRVITPDLAGF
                     GYSGKPRHLDYTIGSQARMIVGLLDALKIDQAIIIGSSYGAAVASTVALDHPDRVQKL
                     ILVGAVTNNNPTRYLLMRLFSSPVIGDIVSPLLLGSRRLLRRRMKRVYDRHSWVLDER
                     RVDARHLPLRAASTHRAIVRTVRRWDADRISRDAHLLKQPTLILWGENDREVPLRDGQ
                     LLLDKIHGSRLVIFRDCGHLPHEEFPLEFTEIVSDFCADKRAKLRAVVG"
     gene            4223..5389
                     /gene="yhbH"
                     /locus_tag="VFV61_07360"
     CDS             4223..5389
                     /gene="yhbH"
                     /locus_tag="VFV61_07360"
                     /inference="COORDINATES: protein motif:HMM:TIGR02877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YhbH"
                     /protein_id="HEX4900335.1"
                     /translation="MSIQRNDWSLQRKGIIDQERHKERVKEAIRKNLGSIVSNEAIIL
                     SDGKRTVKVPIRALDEYKFRFDYRKKKHVGQGDGKTRVGDVLGREGQPGQGTGGGQAG
                     AEGGAEYYEAEVNIDEIAALIFEDLHLPFLEEKAKQAVQSKKTQFNEIRRTGAIANLD
                     KRRMILENIRRNAREGQGARFGDIKKEDLRFKTWEEVMRYESNAVVIAMIDVSGSMGE
                     FKKYVARSFYFWMVRFLRTKYDNVEIVFISHHTEAKEVTEEQFFTQGESGGTVVSSAY
                     QLALDIIKQRYRPADWNIYPFHFSDGDNYYSDNEEAVRLAEELISTCNLFGYGEIGEE
                     GSSSYRRSSGALLSIFSDRIKHKERFVGVRIDDKEDVYPALKQFFGKRGIETPA"
     gene            5430..>6308
                     /locus_tag="VFV61_07365"
     CDS             5430..>6308
                     /locus_tag="VFV61_07365"
                     /inference="COORDINATES: protein motif:HMM:NF016201.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HEX4900336.1"
                     /translation="MEDREIKNLEKSVEEIWEIARRFGLDPFPTNFEIVPASVMYEVG
                     SYALPGRYSHWTFGKAYHRMKTMYDFGLSKIYEVVINTNPAYGFLLETNSPVQNKMVI
                     AHVLGHVDFFKNNVYFSKTNRRMVESVSTHSRRIMEYEFKHGRKAVEKFLDAVLAIEE
                     HIDPNFFIKRNITPEEERRRAELPSKEGRYDDLWNLEPPAEGDVLSSNATLKQENDLP
                     AERLPEKDIVFYIMKNSPALTSWQRDLIAMVHEEMEYFVPQMQTKIMNEGWASFWHAR
                     IMRELDLSDQEHIEFAD"
BASE COUNT         1569 a         1606 c         1736 g         1397 t
ORIGIN      
        1 ttatcgacaa acctgccggt atgacttcgc acgacgtggt tcagcgtgtt aggcgccttt
       61 tgggtgaacg gcgaatcggc cacaccggca cgcttgatcc ttttgctacc ggcgtgctcg
      121 ttctgctgat tggtcgggca actcgcttag cacaatttct ttcgggtgca gacaaagaat
      181 acgaagcgac aattcgcctg ggatttgcca ccgacactgg cgaccgagaa ggaaatccta
      241 tcagcgaaaa attgaagact cagacaacgt ggtcagatga tgagatcaga aatgctctgc
      301 aaagactgcg aggcgagatt cttcaaatgc ctccaatgta ctccgcgaaa aaacgcgcag
      361 gccaacggct gtacaagctg gcgcgtaaag gagaacagat cgagcgcatg ccagtgcccg
      421 taaccatcca caacctcgaa cctctcattg ccaatgccgg cttgctcaaa agcaatgacg
      481 atggaacttc ggacgttcag gtacgcgtag tttgctcggc cggcacttac gtacgtacgc
      541 tggccgaaga ttttggtaag ctgcttggcg taggcgcaca cctcacggaa ctgcgtcgga
      601 cgcgtgctgg ggattttctg ttgcgggatg ccgccaccct ggaacaactg cagcagcgga
      661 ctgaggagga ttccctcagg actatcctat tgcctccgga cgcggcgctc tccagtatgc
      721 cttttctgta tctaagtgct gaagacgtgc aaagggtgcg tcacggcatg gcccttcctc
      781 ggcccgcgga aatctggcgt gagggcgaga aggtaaggat gaaggacgag catgatgagc
      841 tgattgcgat tggcgtatat gactccggca atcggttgct tcgtccgagg gtcgtgttag
      901 cgcaagaaaa ttttgattga ctcacctgcc cgtacagcgt gctatactgc gatcaactgc
      961 gggagcgcgc gggccgcttc caaaaaatgt cactcctccc cggcgcctcc cattgaatag
     1021 ctcattgata gagtaaggcc tcgaacatgc aggtcacgct tacgcgcgcg tggtcccaaa
     1081 cgcatgggcg aggtcgatat ttggcaacca gttttccaga gactggtcgc tatgtaaggc
     1141 gtgctttacc ccgacacgcc cctccatgcg cgcaaaagcc acgaggtttt aaggctatgg
     1201 aagagcagga tcccaacaaa caattcaata tcagtgaacg cttggaagat cttcgtcgcg
     1261 agcatgaagc tctgcgttgg gaagggacct tccgcgatta tttcgagctg gtcactcaga
     1321 atcctcgcat cgcgcaactc tcgcacgcaa gaatcaatga catgattcat gcggccggaa
     1381 tcgagcgctt gaatgaaggc actcatcatg agctgatctc ctacaacttc ttctcttccg
     1441 aactctttgg catcgaagag ccgatcgcca gattggttga gtacttcaag tcagccgctc
     1501 aaaggctcga agtgcgcaag cggatcctgc tgctcatggg acccgtcggc ggtggtaagt
     1561 caacgatcgt cagcatgctc aagcgtggcc ttgaggattg gaccaacacc gacgaagggg
     1621 ccgtctacgc gatcaaggac tgtccgatgc acgaagagcc attgcatctg gttcctcatc
     1681 agttacgccc tgaaatcgag aaacattacg gcatctatat tgaaggtgat ctgtgtccac
     1741 agtgccggta ctcactcgaa cacagctacg gcggtcgcca tgaagatgtg aaggtccacc
     1801 gggttctctt ctcagagaag gaacgcgtcg gcatcgggac tttctcgccg agcgatccca
     1861 aatcacagga catcaccgag ctgaccggtt cgatcgatct ctctacgatc ggtgagattg
     1921 gggttgagtc agatccacga gcctaccgct ttgatggcga actcaatatc gcaaaccggg
     1981 gcttgatgga attcgtcgag atgctgaagg tcgatgaaaa gttcctctat agcctgctca
     2041 ctctttcaca ggaacaaagc atcaaaaccg gccgttttgc gatgatttat gccgacgagg
     2101 tcatcctgtc gcattcaaat gagaatgaat acatcgcctt tgccggcaac cgtaaatcag
     2161 aggccttgca agaccgcata atcctggtac gggtccccta caacctccgg gtctctcagg
     2221 aagagcggat ttacgacaag ctgctgcatc aatccgaagt gctgcgaaac gttcacctgg
     2281 cgccaaacac actgagagtc gccgccatgt ttgcggtaat gacccggctg gaggaaccaa
     2341 agcgccagaa cgtagacgtc gcaaagaaga tgaagctcta tgacggcgag gatgtcgaag
     2401 ggtacaaatc caaagacgtg cgcgaactga aagaggaaac cgttcgcgaa ggaatggacg
     2461 ggatctcgcc acgctacatc atcaatcgcc tctccggagc tttggtgcgc gacggagtta
     2521 cctgtatcaa cccgatcgat gctttaaggg ccatcaagga cggttttgaa cagcacaccg
     2581 gcattagcgc cgatcaacgt gagcgctatc tgaacatgat ttcgctggct cgcaaggagt
     2641 atgacgagct cgccaagatc gaagttcaac gagcgtttgt ttattcgttt gaagagatgg
     2701 ctcgcacaat gtgtaataac tatctggata acgttgaggc ctattgcaac aaagaacgga
     2761 taaaggatcc gattaccgaa gaagagatgg atccggatga gcaattaatg cgttccatcg
     2821 aggagcaaat cggaatttcc gacaacgcta agaatacctt ccgccaggag attctgattc
     2881 gcatctcgtc atacgcacgt aaagggaagc cgtttgagta caattcgcat gaacggctaa
     2941 aagaagctat cgagaagaag atctttgcgg atctcaagga tgtcgttaag atcaccacct
     3001 cagccaagac tcccgatccc gatcagttgc ggaagatcaa tgaggtcgtg gatcgattgg
     3061 taagagacca cggctattgt cccgtgtgcg cgaacgagct gttgacctac gtgggtacct
     3121 tgctatcgcg ttagtcagag atgtaaagcc gcaagccaac gcccgcgctc tcttctttcg
     3181 agttgcgggc gtttggattg ttccccatga aaaaacgata ccttttcgca ggtctgtcag
     3241 gagtcgtggc ggcggctttt gccacgaaac tcctcattag gccacgcgat gttaattggg
     3301 aagagagccg ggacgtggta ttccacgccg agcattcgcg ttttgtcgct gtcgacggcg
     3361 ttaggattca ctaccaggaa gcaggtgaac gcgatgcgcc cgcgatgatc ttgatccacg
     3421 gttttgcctc gtcaacgctt gtctggagca aggtcttgtt ggagcttgcg gcggagggat
     3481 tcagagttat aacccccgac ctggcggggt ttggttattc gggaaagcct cgccacctcg
     3541 attacaccat cggctcgcag gcacgaatga ttgttggcct gctggacgcg ttaaagatcg
     3601 atcaggcgat catcattggt agttcgtacg gggcagccgt cgcgtcaact gtcgccctcg
     3661 atcatcccga ccgcgtgcag aaactgatcc tggtgggagc agtgacaaac aacaatccaa
     3721 ccagatatct gttgatgcgt ctgtttagct cgccggtgat tggagacatc gtttccccgc
     3781 tgctgctggg ctcacgcaga ctcttgaggc gccgtatgaa gcgagtgtac gaccgccact
     3841 catgggtttt agatgagcga agagttgacg ccaggcatct tcccttgcga gccgcgtcca
     3901 cccatcgcgc gattgtgcgc accgttcggc gctgggatgc ggatcgaatt tcgcgcgacg
     3961 ctcacttgct gaaacagcca acgctgatcc tatggggcga aaatgatcgg gaagtgccgc
     4021 tcagggatgg ccagttgcta ctagacaaga ttcatggctc aaggttggtt atcttccgcg
     4081 actgcggaca tctcccccac gaagagtttc ctcttgaatt taccgaaata gtctccgact
     4141 tctgcgctga taaaagggca aagttgcgcg cagtggtggg ctagtttcag actcttgcca
     4201 ttgcagattg aaggtttttg ttatgtcaat ccaacgaaac gactggtcgc tgcagcgcaa
     4261 aggcatcatc gatcaggaac gtcacaaaga acgcgtcaag gaagccatcc gaaaaaatct
     4321 gggctcgatc gtgtccaatg aagcaatcat tctgtctgat ggcaaacgga cagttaaggt
     4381 gccgatccgc gcgctggatg aatacaagtt tcgttttgac tatcgcaaaa agaaacatgt
     4441 cggccagggg gacggaaaga cgcgggtggg agacgttctg ggaagagaag gtcaaccggg
     4501 tcagggaaca ggaggcgggc aggccggtgc cgaaggggga gctgagtact acgaagccga
     4561 ggtaaacatc gacgagattg cggcgctcat tttcgaagat ctgcatcttc cttttcttga
     4621 ggagaaggca aagcaggcgg tgcaatccaa aaagacacag ttcaatgaga ttcgccgcac
     4681 cggcgcgatt gccaatctcg acaagcgccg catgatcctc gagaacatcc gccgcaatgc
     4741 ccgcgaggga cagggcgcca ggtttggcga tattaagaaa gaggatttgc gtttcaagac
     4801 ctgggaagag gtaatgcgtt acgagtcgaa cgccgtcgta attgccatga tcgacgtttc
     4861 cggctcgatg ggtgaattca agaagtatgt ggcgcgatct ttttactttt ggatggttcg
     4921 ctttctgcgc acaaaatatg acaacgtcga aatagttttc atctctcacc acactgaggc
     4981 aaaggaggtt acggaggagc agtttttcac tcagggtgaa tcaggcggca ccgtcgtttc
     5041 gtccgcttac caacttgcgc tggacataat caagcagcgc taccggccgg cagactggaa
     5101 catctatccc ttccatttct cagatggcga taactattac tcggacaacg aagaggcggt
     5161 gcggctggca gaagaactga tctcgacttg caatctcttt ggctatggag agattggtga
     5221 ggaaggttcg tcgagttaca ggcgctcttc tggtgcctta ctttccatct tcagtgatcg
     5281 tatcaaacac aaagaacggt tcgttggcgt gcgcatcgat gacaaggaag atgtttatcc
     5341 tgcactgaag cagttttttg gaaagcgagg gatagagacg ccggcgtgaa aggaagtgat
     5401 tatcggcaag agccattcgg caatgaccca tggaagatcg cgaaattaaa aacctggaaa
     5461 agtcggtcga ggagatctgg gagatcgctc ggcggtttgg tctcgatccg tttccgacga
     5521 attttgagat cgttcccgct tcagtgatgt atgaggtcgg ttcttacgcg ttgccgggac
     5581 gctactctca ctggaccttc ggtaaggcct atcatcgaat gaaaacgatg tacgactttg
     5641 gcctgtcgaa aatctacgaa gtcgtgatca atacaaatcc ggcctacggg tttctgcttg
     5701 agaccaacag cccggtgcaa aacaagatgg tgatcgcgca cgtcctgggt cacgtcgatt
     5761 tctttaagaa caacgtttac ttctccaaga ccaatcgccg catggtcgaa agcgtttcca
     5821 cccattcgcg ccggatcatg gagtacgagt tcaaacacgg ccgcaaagcc gttgagaagt
     5881 ttctggacgc agtgctggct attgaagagc acatcgatcc gaacttcttc atcaaacgga
     5941 acattactcc cgaagaagag agacgccgtg ccgaactccc ctcgaaggaa ggccgttatg
     6001 acgatctttg gaacctcgaa ccacccgctg aaggagatgt cctgagtagt aatgcgaccc
     6061 ttaagcagga aaatgacctg cccgcggagc gattacccga aaaagatatc gtcttctaca
     6121 tcatgaagaa ctcgccagca ttgacctcat ggcagcgcga cctcatcgca atggttcacg
     6181 aagagatgga atattttgtc ccacagatgc agacgaagat catgaatgag ggctgggcca
     6241 gcttctggca cgcgcgcatc atgcgcgaac tggacctgag cgatcaggaa cacatcgaat
     6301 ttgcagat
//
LOCUS       DASUEX010000432         3110 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_516002, whole genome shotgun sequence.
ACCESSION   DASUEX010000432 DASUEX010000000
VERSION     DASUEX010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3110
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516002"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(186..1505)
                     /locus_tag="VFV61_07370"
     CDS             complement(186..1505)
                     /locus_tag="VFV61_07370"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX4900337.1"
                     /translation="MRFVFSRFFYLLIALGLIPLTLSWGHPWLRWATLAYDLGLLVLA
                     IIDSRTSRLPSGVVINREFSGRFAVGAETEVRIQVSNSTSSTISLILKDEYPPQMKLS
                     GLREAHLSVPAQTTAALIYGLTPPKRGRFEFGQIAVRFLSRLNLVWCDTKAGSADVVK
                     VYPNMRRAREAELKALGARSLVAAHRKTSWRGEGREFESLRDYVRGDELRHISWSATA
                     RRGKLTTRQYQIERDQTILIVLDAGRLMTARIDQETKLDSAVHAALALMSAAARAGDN
                     AGLMVFGRRIKSYLPPSRGHDHLDAALEALHAIEPEMIEPSYSRAFEFVAANSKRRSL
                     VVLLTDLVDEEGSKELLTSLKLLRPRHLPLVVTIADRDLKAVVSGAPETATDLFTQSV
                     AEEIIHHREAALRLVESQGGLAMDVSAAALAPALLEKYLQVKERGLL"
     gene            complement(1545..2498)
                     /locus_tag="VFV61_07375"
     CDS             complement(1545..2498)
                     /locus_tag="VFV61_07375"
                     /inference="COORDINATES: protein motif:HMM:NF040105.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX4900338.1"
                     /translation="MLEYVSQTVDHIRNELRKVIVGQEEPIEQVLVALLAEGHALIEG
                     VPGTAKTLTVKTLARIIRADFGRIQFTPDLMPSDITGTNVFNVATSTFNLRPGPIFTD
                     ILLADEINRTPPKTQAALLEAMEERQVTIDGEGHRLSPIFTVLATENPIEYEGTYPLP
                     EAQLDRFLLKILIDYPAEEDEQQIVANWDAGFNSRRLDQVEIHPLEDADAISRCRREV
                     STAHMEPGVQHYAVDIVRRTRAHPLIHYGASPRASVALLLCSKALAAIRNRDFATPDD
                     VRDVTRPVLRHRLTLRAEAELDGATPDAVITDILKTAEVPR"
     gene            complement(2609..>3110)
                     /locus_tag="VFV61_07380"
     CDS             complement(2609..>3110)
                     /locus_tag="VFV61_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900339.1"
                     /translation="AATGNPFVSYFSGTPILALSAQLVLLVLLILWTQGRRFARPLPL
                     ARVDRRSALEFVASMAEVQQRARAFDLAIENIYSRTRRVLARYAGLAYNSPRVEIATR
                     VAARSSVNGRKLEVLMRQCEEVINGQPVSEKQSITLVKRLREVEATLGLRMRSRDLKQ
                     AGQKIG"
BASE COUNT          699 a          865 c          883 g          663 t
ORIGIN      
        1 gatgatgggt attgcgccgc tactggtact cgcagggatc atagaaggct ttatctcacc
       61 tgccgatatc aatccgaaca tcaaattcag tatcgcaggc attaccggag tggcgctcta
      121 ttcctacttg ttactggccg gccgctcgtc tcatgaacca aaggcgtgag gtaatcattt
      181 gctgctcaga gtaagccccg ctccttgacc tgcaaatact tttcgagaag agcaggcgcg
      241 agtgccgccg ccgagacatc catggccaac ccgccctgcg attcaaccaa acgaagcgcc
      301 gcttcacggt gatggatgat ctcttcggcc actgactggg taaacagatc ggtagctgtt
      361 tctggggcac cactaacaac cgccttcaga tcgcggtcgg caatggtaac caccaaagga
      421 aggtgacgcg gacgcagcag tttcaaagag gtcaacaact ccttcgaacc ctcttcatcg
      481 accaggtccg tcaaaagcac aaccagcgag cgtcgcttgc tattcgccgc gacaaattcg
      541 aacgcgcgcg agtaagacgg ctcaatcatc tccggttcga ttgcgtgcag agcttcgagt
      601 gctgcatcca gatggtcgtg ccctcggctg ggtggcagat agctcttgat gcggcggccg
      661 aaaaccatca agccagcgtt atcgcccgcc ctcgctgctg ccgacattaa ggccagagca
      721 gcatgaaccg ccgaatccag ctttgtttcc tgatcaatac gcgccgtcat tagccggcca
      781 gcgtcgagca cgatcaagat tgtctggtca cgctcgatct gatactggcg tgttgtcagt
      841 tttcctcgtc tcgctgttgc actccaggag atgtgacgta actcatcgcc gcgaacgtag
      901 tcacgcaggg actcaaactc tcgaccttca ccccgccagg aagtctttcg gtgtgctgcc
      961 accagcgagc gtgctccgag cgctttgagt tcggcttcac gtgcgcgccg catgtttgga
     1021 tagaccttca ccacatcggc cgaacccgct tttgtgtcac accagaccag gttcagccgc
     1081 gaaagaaacc gcaccgcaat ctgcccaaac tcaaagcgac cgcgctttgg cggagtcagg
     1141 ccatagatga gagcagccgt cgtttgtgcg ggaacgctga gatgggcctc gcgcaggcct
     1201 gagagtttca tctgcggcgg atactcgtcc ttcaggatta gcgaaatcgt agacgaagtg
     1261 ctgttactga cttgaatgcg tacttcggtt tcagctccaa ctgcaaaacg accactgaac
     1321 tcacgattga tgacaacgcc tgacgggagc cggctggtgc gggaatcaat tatggcgagc
     1381 acaagtaggc ccaggtcgta ggcaagggtg gcccagcgca gccaggggtg accccaggag
     1441 agcgtcaggg gaatcaaacc cagcgcgatg agcaagtaga agaagcgcga gaaaacaaaa
     1501 cgcatgagag cgagactctg ggaagtaatg aaccttttac gaattcatct tggtacttcc
     1561 gctgtcttca gaatgtcggt gatcacagca tcaggtgtgg caccgtccag ttcggcttcc
     1621 gcacgcaacg tcaagcgatg acgcaatacc ggccgcgtga cgtcgcggac atcatcagga
     1681 gtagcaaagt cccggttgcg aatagcagca agcgcttttg agcaaagcaa cagtgcaacg
     1741 gaagcgcgtg gactggcgcc atagtgaata agcggatggg cgcgagtgcg gcggacaatg
     1801 tcaacggcat agtgttgcac gcctggttcc atgtgagcgg tgctcacttc ccggcggcag
     1861 cgagagatgg catctgcatc ttcaagcgga tgaatctcga cctgatccag cctccttgag
     1921 ttaaacccgg cgtcccaatt ggccacgatc tgttgttcgt cctcctcggc cggatagtcg
     1981 atcagtatct tcaacagaaa gcgatctaat tgagcctccg gcagcggata tgttccttcg
     2041 tattcgatcg gattctctgt agccagcacg gtgaagatcg gcgaaagccg gtgtccttcg
     2101 ccatcgatgg tcacctgccg ttcttccatt gcctccagca gggctgcctg cgttttgggc
     2161 ggcgtgcggt taatctcatc cgccagcagg atgtccgtga aaattggccc cggacgtaga
     2221 ttgaaagtgg aggtagctac gttgaagacg ttggtgccgg tgatgtcaga aggcatcaaa
     2281 tccggggtga actgaatgcg tccgaagtct gcccgaatga tccgggccag ggttttgacg
     2341 gtcagtgtct tggcggtgcc gggaacgcct tcgatcagtg cgtggccctc ggcgagcaag
     2401 gcgacgagaa cctgctcaat gggttcttcc tgtccaacga ttacttttcg gagttcattg
     2461 cgtatgtgat cgactgtctg agatacatat tcaagcatta gttatctagt caccctttga
     2521 gaagaaggtg ttcgagcttc ggttgtgatg ggacatgaaa gcccaaagcc atctgttacg
     2581 caagcttgta aagctcacac ttgaatcgtc atccaatctt ttgtcctgcc tgtttcagat
     2641 cccttgaccg catccgcagt ccgagggtcg cctccacttc acgcaggcgc ttgaccagag
     2701 tgatcgattg cttctcgcta actggctgtc cgttgattac ctcttcgcac tggcgcatca
     2761 agacctccag tttgcgccca ttaactgaag agcgcgcagc tacccgcgtg gcgatctcaa
     2821 cgcgcgggct gttgtaagcc aggcccgcgt aacgggccag tacgcgccga gtgcgggaat
     2881 agatgttctc gatggcgaga tcaaaagcgc gcgcgcgttg ttgcacttcc gccattgagg
     2941 caacaaattc caatgccgaa cggcggtcga cccgcgccag gggcaagggc cgagcaaaac
     3001 ggcggccctg agtccagagt attaagagga ccaggagcac gagttgagcg ctgagcgcga
     3061 ggattggcgt acctgagaaa taggaaacaa agggattgcc ggtggccgcg
//
LOCUS       DASUEX010000433         3313 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1118449, whole genome shotgun sequence.
ACCESSION   DASUEX010000433 DASUEX010000000
VERSION     DASUEX010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3313
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1118449"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="VFV61_07385"
     CDS             complement(<1..202)
                     /locus_tag="VFV61_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900340.1"
                     /translation="MIRRQLNTSGSITKYRLAHLWVVIGVLALLTSLLAPDFSNPAAA
                     QRRQRRRPVTSARPGSDYSRFSH"
     gene            complement(228..995)
                     /locus_tag="VFV61_07390"
     CDS             complement(228..995)
                     /locus_tag="VFV61_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900341.1"
                     /translation="MTALALLLLSNLVIAQTPEAARGVIRLRVRVKTGNTTSGLSRKR
                     FFLLPGTIEQNKALLQTIENTTTRSRDCYYRSVGASDPLMKWLREGDCESVYCRGIES
                     ADVEAVPEFQRAFAAGEQEFGNRELARKWLTVNLPENIRDGFYKVRQLELQKLITQAE
                     ASGGKVLSVMTDTKGTAYFTEIPVGTYVISNLLPAEVGSANVYWNCEVKVKAGDLSTE
                     KPFLISNVREGPVKCVATSKPLPACDVAALGSSANRP"
     gene            complement(1141..>3313)
                     /locus_tag="VFV61_07395"
     CDS             complement(1141..>3313)
                     /locus_tag="VFV61_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900342.1"
                     /translation="SLETRSTLTTMVRRMTGSLNVGARRHHAFLPCLLLASLVIVAVV
                     RFPPAGAQTSTPILISEETSTRALVFDSTTREREPFTAHTQVTFGGDARRRLMIFAMH
                     LQLEPGELPSAVVARAEDASHQSYLLPVEHVGPTPGQTWATSIVVRLDPAMGDLGDVL
                     VGITYHGVSSNRVRVGIGHVGGGPPDDPGAVPTPGGIQAPPAATAGNLSTSEVQTIIS
                     QAVSAAVSMNRTVTVAVTDREANVLGVFSMTGAPATTTIRSVGTSGMGLEGSIVPASL
                     AAISKAGTGALFSTGGNAFTTRTAGFIIQEHFPPGIDFRPGGPLYGVQFSSLPCSDIK
                     IPGLPLGLSGDPGGLPIYKDGMAVGGVGIEGDGLYTVDRTPADFDQPAEELIAASAVR
                     GFEAPALIRGDNILVDGIRLPYSNVATPPTPPTIAFGSLPGAIVAGFPIRPAQTSAFV
                     PALVAGIAGEVDPRFFPFVAGTAPAGPNALTAAEVQTIMTQAIQQANITRAAIRQPLG
                     SNGRVTIAVVDANGTVLGLFRQLDAPVFGFDVSVQKARTAAFFSRADAAALLTGAGFG
                     SYVSRAATDGMQLNGTVAFSARAIGFLHRPLFPDGINNTTAGPFSTSLTDWSPFDVGL
                     QLDLIKTNLLATLGGAAVPCSSIPNLPNGVQIFAGSTPLYKNGVLVGAIGISGDGIDQ
                     DDLVSAAGANGFSPAPAIRSDQVFVRSVRLPFLKFPRSPNL"
BASE COUNT          750 a          916 c          974 g          673 t
ORIGIN      
        1 tatgtgaaaa cctggagtag tcgctgcccg gccgcgcact cgtcacgggt ctgcgacgtt
       61 ggcgtcgttg tgccgcggca ggattgctga agtctggggc aagcagggaa gtcaggagcg
      121 ccaacacacc aatcaccacc cacaaatgtg cgaggcggta ttttgttatt gatccagatg
      181 tgttgagttg gcgtctaatc aagatgtagt cacgggccac cggggaatca gggccggttt
      241 gcagacgagc ccagcgccgc aacatcacac gccggcagtg gtttcgaggt ggccacgcat
      301 ttcaccggac cttcccgtac gttcgaaatc agaaacggct tttctgttga gagatcgccc
      361 gccttaactt tgacttcgca gttccagtaa acatttgcac tgccgacttc ggcaggcagt
      421 agatttgaaa tgacgtaggt gccaacaggt atttctgtga agtatgccgt gcctttggta
      481 tcagtcatca cggagagaac cttgccgccg gatgcttccg cttgggtgat cagtttttgt
      541 agttcaagtt ggcgcacctt atagaaaccg tctctgatgt tttctggcag gttcaccgtt
      601 aaccacttgc gcgccagctc gcgatttccg aattcctgtt cacctgcggc gaaagcccgc
      661 tgaaattccg gcacagcttc gacatccgcc gattcaatcc cgcggcagta gaccgactcg
      721 caatcgcctt ctcggagcca tttcatcagc ggatcgctcg ctcccacgct gcggtaataa
      781 caatcgcgtg atctcgtggt tgtgttttcg attgtttgca gcagggcttt gttttgttca
      841 atcgtgccgg ggagcaggaa aaaacgcttg cgtgagagtc cgctggtggt gttacccgtc
      901 ttaacccgca ctcgcaatct gattacgccg cgagcggcct ctggtgtttg cgcgatgacc
      961 aggtttgaga gcaacagcag tgcaagggcc gtcacagtaa gggaaagttg taagcggcgg
     1021 ataagactca agcggcggca agccggcctt ccgaactgag catcttcacc agcatttttc
     1081 aacggagcgt ttttcacaag gagacgcata acaagctaag ggcaatgctc tcgccagcga
     1141 ctaaagattc ggactacgag gaaacttcaa gaaaggaagc cgcactgatc taacgaaaac
     1201 ctgatctgaa cggattgcgg gcgccggcga aaaaccgttt gcacctgcgg ccgaaacgag
     1261 atcgtcctga tcgatcccgt ctccactgat gccaatcgca ccgacgagga cgccattttt
     1321 atagagcggt gtactgccgg caaatatctg cactccgttc ggcagattcg gaatggatga
     1381 acagggaacc gcggcgccgc cgagtgtggc tagcagatta gtcttaatta aatccaattg
     1441 cagaccgacg tcgaagggac tccagtctgt caacgacgtg ctgaagggac ctgctgtggt
     1501 gttgttaatc ccgtcgggga acaagggccg atggagaaaa ccgattgcgc gagcgctaaa
     1561 agctacggtt ccgtttaatt gcatcccatc cgtggcggcg cgacttacat aggaaccaaa
     1621 gccggcacca gtcagtaagg ctgcggcatc ggctcgcgaa aagaaagcag cggttcgtgc
     1681 cttctgcacg gaaacgtcaa aaccaaaaac cggtgcatcc agttgacgga agagcccgag
     1741 caccgtgccg ttggcgtcga caacggcgat cgtcacccga ccgttgctac cgagaggttg
     1801 gcgaatcgcg gcgcgcgtaa tgttcgcttg ctgaattgcc tgagtcatga tcgtttgcac
     1861 ttcagcagcc gttagagcat tcggacctgc gggagcagta ccggcaacaa agggaaagaa
     1921 gcgtggatcc acttcgccgg caataccggc aacaagggcg gggacaaagg ccgaagtctg
     1981 cgcgggtcga atcgggaagc cggcgacaat tgccccgggc agactgccga aagcgattgt
     2041 gggcggtgtc ggtggagttg ccacgttgct gtaaggaagc cggattccgt ctaccagaat
     2101 gttgtcaccg cggatcagtg ccggcgcttc gaagcctcgt accgcagagg cagcgatcag
     2161 ttcctcggca ggctgatcaa agtcggcggg agtgcgatcg accgtgtaca aaccatcgcc
     2221 ttcaatgcca acgcctccaa ccgccatacc gtctttgtag attggcaggc caccagggtc
     2281 gcctgagaga cccaatggca gtccgggaat tttgatatca gagcagggca gtgaggagaa
     2341 ctgaacgcca tagagcggac ctccagggcg aaaatcaatt cccggtggaa agtgctcttg
     2401 aatgatgaat ccggcagttc gagtcgtgaa ggcgttcccg ccggtgctga aaagtgcgcc
     2461 tgtcccggct ttcgaaatcg cggccaggga agccggcacg atgctgcctt cgagtcccat
     2521 cccggacgta cccacgcttc taatcgttgt agttgcagga gccccagtca tgctgaacac
     2581 tccaagcacg ttggcttcgc gatcagttac ggcgaccgtc actgtgcgat tcattgaaac
     2641 cgctgccgac accgcctggc taatgatggt ttgcacctca ctcgtgctga gattcccggc
     2701 tgtggcggca ggaggcgcct ggatacctcc gggagtcggc accgcgccgg gatcgtcggg
     2761 agggccgcca ccgacgtgcc caattcccac tcgcacacga ttgctggaaa caccgtgata
     2821 ggtgatgccg acgagcacgt cgcccaggtc gcccatcgcc gggtcaagtc taacgacaat
     2881 cgaggtggcc cacgtttgtc cgggagttgg tccgacgtgc tccaccggca acaggtaact
     2941 ctgatgagaa gcatcttcgg cgcgcgccac taccgcgctc ggcagctccc cgggttcaag
     3001 ttgcaagtgc atggcgaaga tcatcagccg gcggcgcgcg tcgcctccaa aggtgacttg
     3061 cgtgtgggca gtaaaaggtt cgcgctctct cgtggtcgaa tcaaaaacga gagcgcgagt
     3121 cgaagtctct tcagaaatca gaatgggagt tgaggtttgc gccccagcgg gagggaagcg
     3181 aacaacggcg acgatgacta aagacgccaa cagcaggcaa ggcaaaaagg catgatgcct
     3241 gcgggcgcca acattaagac taccagtcat cctccgcacc atcgttgtta aggttgatct
     3301 agtctcgagg ctt
//
LOCUS       DASUEX010000434         2188 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_84319, whole genome shotgun sequence.
ACCESSION   DASUEX010000434 DASUEX010000000
VERSION     DASUEX010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84319"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            303..1142
                     /locus_tag="VFV61_07400"
     CDS             303..1142
                     /locus_tag="VFV61_07400"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEX4900343.1"
                     /translation="MKRRFPFIVVLVIFTAPVALAQNAAYSFDNFDTSSGVKIHTVPV
                     STQLTIAKPTLGSKRSGVAAASTDAKQPSASLQRTSLRFEPVMTAGSVASSLRGFTTG
                     NEQVDGYIMESASRNGLDPLLIYSIMHQESSFKQRAISYKGARGLMQLMPGTAARYGV
                     TNIFDPKQNIEGGSRYMKFLLNRFQGDLSLALAGYNAGEGAVDKYGWRIPPYAETQEY
                     VRRISRRYNLLRDPNAAIYAPTVSRSQVARLNAKQSAPLNIYERSVLTVRLPDGRLQL
                     MTQ"
     gene            1357..1857
                     /locus_tag="VFV61_07405"
     CDS             1357..1857
                     /locus_tag="VFV61_07405"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEX4900344.1"
                     /translation="MRTEGPAAAQRYGEDADVENVVFTVRPTLLFVKIGYVAAIVGAI
                     VLTILLAMVSFIDIPIYLSLPIALALLLIPAYYHIRRNMVRYTLTDSKIEIDYGLIAR
                     TTRNVPLSKIQDVTVSASILQRILGFGDVVIDNASEFGGSTILHNINNPRHYADLLLR
                     ELRRWH"
     gene            1947..>2188
                     /locus_tag="VFV61_07410"
     CDS             1947..>2188
                     /locus_tag="VFV61_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900345.1"
                     /translation="MRRFLFAAIFTLSAAAFASAQPQLASPQPLPAEDQTPVLIKHLP
                     NWETAGKNARYIVTLDELKRNVVNQPIVDSVSFEGGT"
BASE COUNT          498 a          606 c          606 g          478 t
ORIGIN      
        1 caactgcggt caccgcacac aaaaggatga ccgagatgat tagtaacggg agtttcttca
       61 tatcttctcc ttagtttcgg gaggcgcccg aaagcggacg cgaagttgat aaagccggga
      121 aagccttgaa aaaattgagg cgccatcata ctacccaagt caacgcaagg ttttttacat
      181 ggatcttaag gccatcatcg gccaagacgg ttggcaaatc tttaacactt tggtcgtatc
      241 ggtttacccg cgaaatgcgg gaaaacctcg aaggcctgcc ctaagccagt cacagcatcc
      301 caatgaaacg cagattcccc tttatcgtcg ttctggtaat tttcacagcg ccggttgcat
      361 tggcgcaaaa cgctgcctat tccttcgaca atttcgatac ctccagcggg gtcaaaatcc
      421 atacggtgcc ggtgagcacg cagctgacca ttgccaagcc gacactcgga tcaaagagat
      481 ccggggtcgc ggcggcttcc acggacgcaa agcagccttc agcttcactc cagcggacgt
      541 cccttaggtt tgagccagtc atgacggccg gcagcgtggc atcgtcgctg cgcggcttca
      601 cgacgggaaa tgagcaggtt gacggctaca taatggaatc ggcctcacgc aacggactgg
      661 atccgctgtt gatttattcg atcatgcatc aggaatcgag cttcaagcag cgcgcaatat
      721 cttacaaagg tgcgcggggc ttgatgcagt tgatgccggg aacggcagct cgctatggcg
      781 tcacgaatat tttcgatccg aagcaaaaca tcgaaggcgg ttctcgctac atgaagttcc
      841 tgctgaatcg ttttcagggg gatttgtcgc tggcgctggc cggttataac gccggtgaag
      901 gcgcagtcga caagtacgga tggcgcattc ctccgtacgc ggaaacccag gagtacgtgc
      961 gacgcatctc gcgccgctac aatttgctgc gcgacccgaa cgccgcgatc tacgcgccga
     1021 ccgtgtcgcg ttcgcaagtg gcgagattga acgcgaagca gtccgcgcca ctgaacattt
     1081 atgagcgcag cgtcttgact gtgcgtctgc cggacggccg gttgcagttg atgacgcagt
     1141 aatctcttat caacggattc tctgaacgag gcgatggtcg aaccggacgt cgcctcgttt
     1201 cgtatttgga gaaagagtca ccttatgtat gattagcgct gctcaggagc cagaaacaag
     1261 atgcgctgcg taaattgcgg ttcatttctc gattcagaca gccggttctg caagaactgc
     1321 gggacagcgg tgcgcgaccc cgaagaaacc aggatcgtga gaacggaagg gcccgcggcg
     1381 gcccaacgat atggcgaaga cgccgatgtc gaaaacgtgg tcttcaccgt gcggccaact
     1441 ctgctgttcg taaagatcgg ctacgttgcg gcgatcgtcg gcgcgatagt gttgacgatt
     1501 ctgctggcga tggtcagctt catcgacatt ccaatctatt tatcgctgcc catcgcgctc
     1561 gccctgctcc tgattccggc ttactaccac attaggcgca acatggttcg ttacacgctg
     1621 acggactcga aaatcgaaat cgattacggc ctgatcgcgc ggacgacgcg caacgtgccg
     1681 ctttcgaaga ttcaggacgt caccgtatct gcatcgatac tgcaaagaat tctcggcttc
     1741 ggcgatgtcg tcatcgacaa cgcgagcgag tttggcggct cgaccatcct gcacaacatc
     1801 aacaatccac ggcactacgc cgacctgctt ttgcgcgagc tgcgccgctg gcattaaact
     1861 caacccgggc gactcgcaac tttccacgtt ccgggaacat cgcattgacg gcgggttcta
     1921 atcgagtctg gctgaggtaa tttgaagtgc gcaggtttct gtttgcagca atatttacgt
     1981 tgagtgcagc cgcgtttgcg tccgcgcagc cgcagcttgc ttcgccgcag ccgttgccgg
     2041 ctgaggacca aacccctgtc ctgattaagc accttccaaa ttgggagacg gccggcaaga
     2101 acgccaggta catcgtgacg ctcgatgaac tgaagcggaa tgtcgtcaat cagccgattg
     2161 tcgattcagt cagttttgaa ggcggcac
//
LOCUS       DASUEX010000435         6509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1451864, whole genome shotgun sequence.
ACCESSION   DASUEX010000435 DASUEX010000000
VERSION     DASUEX010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6509
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1451864"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /locus_tag="VFV61_07415"
     CDS             complement(<1..635)
                     /locus_tag="VFV61_07415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900346.1"
                     /translation="MSKEQKKVGQGWLSPARFQLNVDLGFNAVVSPASTLKLQTDRPS
                     HSKAWSMPRPKTMIRLFGPLAILFSILTLSELTAPTKSIPANQLPVDAEASSKTLAAS
                     GNTISVNSAITYQTIIGWEAVAQAGQTDFSDWNSYKASLLDQAVADGINRIKINARTN
                     TEAVTVPGVAVNDNADPFVINAAGFNWATVDAYADVANQLKTRLASQGESL"
     gene            complement(924..6476)
                     /locus_tag="VFV61_07420"
     CDS             complement(924..6476)
                     /locus_tag="VFV61_07420"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX4900347.1"
                     /translation="MAIKLIPPTLLSPEMEERFQREAQLVAQMDHPAVVPIFDFGKHE
                     GSLFFVMPVVQGTNLRYFQRENALTLGEVLDIGIQVAEALEYSHSRGVIHRDIKPENI
                     MVVREDAARVRVRIMDFGLARGATESRITKTGTIAGTLSYMSPEQVAASGVDHRSDIY
                     SLGTVLYECLTGEPPFSGELQSILYRIVHEIPQSPRSMGADINEDLEAVILNCIAKDP
                     GKRSQRASEVADSLRRCQTRLHQSDLGKSVVLTKTLMLTRVAPSPFIGREKEQAELQK
                     RLNAAVSGECQFVVVSGEPGVGKTRLLDEIENLANARKLMVLHGRSIEQDGAFPYQGF
                     CEAIQEYFRQRDTTSAPDTRLELSDVAPDLVSLFPMLTEISEIRAAATGDSKLAQTGS
                     SQGPENRTQVFELLARTLTRLAAGKPLILFLEDLHAAEISIEALQYIVRRLGPTPTLI
                     VGTYRTSEIAGRSHPLLRMLDSFRGDRRFSAIALGPFSPAEHRLYLETLIGGPKLADS
                     LVEKLFEGTEGNPFFTKELVRSLLDSGGISKDQTGAWSLGAATDLSTGDLPATIQQAV
                     ERRIERLPEELREVLSIASVIGKAFDSRDLEVLAAGKDVEGAIDRLVEEGLLEEERES
                     RGDRLAFSSGVVRDVLYGAITRRKRRSLHRKYAEEVEKRHGGRLERVYPQLVHHFSQG
                     DVPDKTVEYGLRMAKAALDAFSAEEAARAAKTVLEFLDEEWEGDPALEGDARLLLAQA
                     YRISGDVDAALKEAESAGRILAREDHKSRAVLALLFAAETAWQARKVEETTQLVQQVM
                     DAARAAGDSDSLRHALSLAATLANLRGEYEKANAYLEEAAAQAPAAKETEFKEEIPRG
                     GRLVVALATPLGDIDPVNMELVEEQEILANVFETLLVTDLEGNLVPSLCEKWEVSESG
                     TSVFLTLRENVSFQNGSALTASSIKRSFERSIKVARELPPGLAAISGAAAFAKSDDRE
                     LSGILVHSDYKLEIQLPEPLSIYPALLTDYKTGVTLADGDDGNKDAIQIGTGPFRMAS
                     YSPDRVVLERNKNYWKGSAAFLDEVEFRAGLSASDIASGLRSGSIDLARDLSPQDLEE
                     FLRDPRFRGGLVESPRKNTYFLIFNSTTGSVAEKADLRRALSGIVRTHDLVWQTLGRF
                     AQPAVCLIPPGLLGYDPGRRRQTLTRDEALSMLRSSGVSNQINLRAAVHPLFLDRYKS
                     LLTTLLAIWSDLGVNVEVATPTMASYLESFQNPAGIDVFIGRWNADYDDPDDFTYGLF
                     HSRVGLYRSYISAAEGDQMLEEARTESRPGVRASLYRKYESFLQESGMVLPLFHDVDY
                     RLANSKVLGLTLHGSAPYVNYAEIGKLESGGKATDTQRAGGGTIEVPITGTLRELDPA
                     LAGTVEDAEVLPGIFETLTRDIGGARIVPWLASEFTAEQGGKRYRFRLRDDVRFHDGR
                     KLTARDVRYSFERLLLMPESTGRFFYSSIRGAKALINRERLDLAGFRIHSADEFTIEL
                     EEPVSFFPALISYHAAAIVPEGSDKFGGSWREGSVGTGAFRVVKFEPGLRLELERNRT
                     YWRSGYPRSERLIFNFNVPPADILSQFHAGRFSLASDLLPADAEALRREPEFASGYHE
                     VPSLITYYATFNIHRGPLKDKRLRQILAQSIDVASVVRQTLGRLAVPAHGLIPPGLLG
                     HDSAYSPRMPLASASTPAQPGGEIELTAVLNPVFFGEYAAFAREISRSLRERQITIRP
                     VNKTMEEWREAVSQGSVDVVIGRWGADYPDADTFANILATKEGLLGQVCGSAEVDRLI
                     ARGRSETSPAARHEIYRQIEEIIARDRLLLPLFHEQTYRFARPEVEGLSLSYGSIAVD
                     YASLRINA"
BASE COUNT         1449 a         1851 c         1750 g         1459 t
ORIGIN      
        1 taaagggatt cgccctggga ggccaatctt gttttgagtt gattcgctac atcagcgtat
       61 gcgtccactg ttgcccagtt aaatcccgcc gcgttgatga caaacggatc ggcattgtcg
      121 tttaccgcaa caccgggaac cgtgactgcc tccgtgttag ttctcgcatt gatcttaatt
      181 cggttgatgc catccgcaac tgcttgatct aagagtgaag ctttatagct attccaatcg
      241 gagaaatctg tttggcctgc ctgtgccacg gcctcccaac ctattatcgt ctgataggtt
      301 attgcggaat tcacgctgat tgtatttccc gacgcagcaa gtgtcttaga tgaggcttct
      361 gcatcaaccg gaagttgatt agcgggtatg ctttttgtcg gggctgtgag ttccgacaga
      421 gtcaagattg aaaacaggat tgcaagtggc ccaaataagc gaatcatggt ttttggtctt
      481 ggcatactcc aggccttaga gtgacttgga cggtcagtct gtaacttaag agtgctggct
      541 ggactgacca ctgcattgaa acccaggtca acattcaatt gaaagcgagc agggctcaac
      601 cacccctgac ccactttctt ttgctcttta gacaagcgcc tgacagatca gcaaagagga
      661 cacccaggaa ctttgctaag cgaactgaat tttcacgatt cttgttgcgg tcatcgcccc
      721 atggccaccc gaaaaaggcc gttccaagct gcatgttaca acacttgtag acacttttaa
      781 ggtctttctg aacacttggc gtgggcgatg gaccgtctcc tcttatgaaa cggtgcctat
      841 attatttgtg ttgttattgg aggcggggat cgcaatcgaa tcgatgcata aaggctttgc
      901 agaagttgat tggtacacat ctatcacgcg ttgattcgca aacttgcata gtcaacggcg
      961 atggaaccat aagataagga gagaccctcg acttccggtc gcgcaaaacg gtaagtctgc
     1021 tcgtgaaaga gaggcagcag tagtctgtcg cgggcaatga tctcttcgat ctgcctatag
     1081 atctcatgac gcgccgccgg agatgtttct gatcggcccc ttgcaatcag gcgatccact
     1141 tcggctgaac cacatacctg tcccaataat ccctcttttg ttgccaggat gttggcgaag
     1201 gtgtcggcgt caggatagtc agcaccccag cgtccgatca ccacgtcaac agaaccctga
     1261 gatactgctt cccgccactc ctccatcgtc ttattcacag gacgaattgt tatctgacgt
     1321 tcacgcagcg agcgggaaat ttcacgcgcg aaggcggcgt attcaccaaa gaacactggg
     1381 ttgagaacag ctgttaactc aatttcaccg cccggttgcg cgggcgttga tgcggaggca
     1441 agcggcattc ttgggctgta tgctgaatcg tgacccagca gccctggcgg aatcaaaccg
     1501 tgggcgggga cggcaagtcg tcctaaagtc tgacgaacca cgctcgcgac atcgattgac
     1561 tgagccagga tctgccgtag gcgtttgtcc ttgagcggtc cgcgatgaat attaaatgtt
     1621 gcgtagtagg tgatgagact aggaacttca tgatagccgg aagcaaactc gggctcgcgc
     1681 cgcagagcct cagcatcggc gggcaataga tccgaggcca aagagaatcg acccgcgtgg
     1741 aactgcgaga ggatgtccgc cgggggaacg ttgaagttga agataaggcg ttcactcctg
     1801 ggatagccgc tgcgccagta agttcggttt cgttcgagtt cgagcctcag acccggttca
     1861 aacttcacca cgcgaaaagc acccgtaccc acgctgcctt cccgccagct tccaccgaac
     1921 ttgtcactac cttcaggaac gatggcggca gcgtgataag agatcagcgc tgggaagaac
     1981 gagaccggct cttcgagctc gattgtaaac tcgtcagcag agtgaatgcg gaagcccgct
     2041 agatccaacc tttcgcggtt tatgagtgcc ttcgccccgc ggatagacga atagaaaaag
     2101 cggccagtgc tctccggcat caggagtagc cgctcgaacg aatagcgcac atcgcgggct
     2161 gtcagtttgc gcccatcatg aaagcgaaca tcatcgcgga ggcggaaacg atatctctta
     2221 ccgccctgtt cggctgtgaa ctcagatgcc aaccagggaa cgatgcgcgc accaccgata
     2281 tcgcgagtta acgtttcaaa aatgccgggc aacacctcag catcttcaac cgtgcctgcc
     2341 aatgcgggat ccaattcacg cagtgttccg gtaattggga cttcaatggt gccgccgccc
     2401 gcgcgctgtg tatcggttgc tttgcccccc gattctaatt tgccgatctc ggcataattc
     2461 acgtatggcg cgctaccatg aagcgttaga ccgagcacct tcgagttggc aagacggtaa
     2521 tcaacgtcgt ggaatagcgg cagcaccatt cccgactcct gcaagaaact ttcgtacttt
     2581 cgataaaggg aagctctgac acctggacgg ctttcggtgc gggcctcttc gagcatctgg
     2641 tcgccttccg cggcggagat gtacgagcga taaaggccaa cgcgcgaatg gaagagacca
     2701 taggtaaagt cgtcgggatc gtcgtagtca gcattccacc tgccgatgaa cacatctatc
     2761 cctgcgggat tctgaaagga ttccaggtag gaggccattg tgggtgttgc gacttccaca
     2821 ttcaccccga gatccgacca gatcgcgagc agcgttgtga gtagtgactt atatcgatcc
     2881 agaaacagcg gatgcacggc cgctctgagg ttgatttgat tgctgacgcc ggatgaccgc
     2941 agcattgata aggcttcgtc gcgagtcagc gtctggcgcc gccggcccgg atcatatccc
     3001 agcagccctg gtgggatcag acagacggcc ggctgcgcaa agcgccccag tgtctgccaa
     3061 accagatcgt gcgtgcgcac tattcctgaa agcgcccgtc gcaggtctgc tttctcggca
     3121 actgaaccag ttgtcgaatt aaagattagg aaataagtat tcttgcgtgg cgactctacc
     3181 aggccgccac gaaaacgtgg gtcgcggaga aactcttcaa gatcctgggg cgatagatcg
     3241 cgtgccagat caattgaacc tgagcgcaac cccgaggcta tgtctgaggc gcttaacccc
     3301 gcgcgaaatt cgacctcatc cagaaacgca gccgagccct tccaatagtt cttattccgt
     3361 tcaagcacga cgcggtcagg gctgtatgag gccatccgaa acggacctgt accaatctga
     3421 atagcatcct tgtttccgtc atcaccatcg gccagcgtga ccccagtctt gtaatctgtc
     3481 agcaaggcgg gatagatcga aagcggctca ggcagttgta tttccaattt gtagtctgag
     3541 tgcacaagga tgccggataa ttcacgatca tcgctttttg caaatgcagc ggcgccacta
     3601 atggccgcga gtcccggggg tagctcccgc gccaccttaa tactacgttc gaacgaacgc
     3661 ttaatcgacg aagcggtcaa ggccgagcca ttctgaaagc tcacgttttc tcgtagcgtc
     3721 aagaatacag aagtgccgct ctcggacact tcccattttt cgcacagaga gggaaccagg
     3781 ttgccttcga gatcggttac cagcagcgtc tcaaagacgt tcgccaaaat ctcctgttct
     3841 tccactaact ccatattgac gggatctata tcgcccagcg gtgtggccaa agcgacaact
     3901 aatcgaccgc ctcttggtat ctcttctttg aattccgtct cctttgctgc cggggcttgc
     3961 gcggccgctt cttccagata cgcgttagcc ttttcatatt caccccgcag gttggcgagc
     4021 gtcgcggcca gagatagcgc gtgcctcagg ctatcgctgt ctccagccgc acgcgcggca
     4081 tccataacct gctggactag ttgcgtcgtc tcctcgacct ttctggcttg ccaggcagtt
     4141 tcggcagcga acagcagcgc gagcacggcg cgcgacttat ggtcctcacg tgccaggatt
     4201 cgtccagcgc tttcagcttc tttcaatgcg gcatcgacgt cgccggaaat gcgataagcc
     4261 tgggccagca acagccgcgc atctccctcc agggccgggt caccttccca ttcctcatcg
     4321 agaaactcga gtactgtctt cgctgcgcgc gccgcctctt cagcgctgaa agcatcgagc
     4381 gcggctttgg ccatgcgcaa gccatactca acggtcttgt cgggtacgtc gccctgggag
     4441 aagtggtgaa ctaactgtgg gtagactcgc tcaagcctgc caccgtgccg cttctcgacc
     4501 tcttcggcat acttgcgatg caacgacctg cgcttgcggc gagtaatcgc accgtaaagc
     4561 acatcacgaa cgacaccgct cgagaacgcc aaccgatccc cgcgcgactc ccgctcttcc
     4621 tcgagaagtc cttcctctac gagccgatca atggcaccct cgacgtcctt gccagcggcc
     4681 agcacttcta aatcgcgaga gtcaaaagcc ttgccgatta cggatgcaat cgagaggacc
     4741 tcacggagtt cttcaggcaa acgctcaata cgtctttcaa cggcctgctg aattgtcgcg
     4801 ggcagatcgc cggttgagag gtcagtcgcc gcacctaaac tccacgcccc ggtttggtct
     4861 tttgagatgc cgcccgaatc taacagggag cgcaccagtt ccttcgtgaa gaacgggtta
     4921 ccttccgttc cctcaaagag tttctctacg agactgtcag ctagtttggg accgccaatc
     4981 agtgtttcca gatacaggcg atgttcagcg ggtgagaacg gtcccagcgc gatcgctgaa
     5041 aaacggcggt cgccgcggaa actgtcgagc atacgcagta gcggatggga gcggcccgcg
     5101 atttcactag tgcggtaggt acccactatg agggtgggtg tcggccccag tcgccgcacg
     5161 atgtactgca atgcctcgat ggagatctct gccgcatgaa gatcttcgag aaacaggatc
     5221 agcggctttc ctgccgccaa tcgggtcaag gtccgcgcga gtagttcaaa gacctgggtg
     5281 cggttttcag gaccctgcga gctacccgtc tgggccaact tcgaatcgcc cgtcgccgcc
     5341 gcccgaattt cactgatctc tgtcagcatc ggaaatagcg acacgagatc aggggcaacg
     5401 tctgatagct caagcctggt gtctggagcg ctggtcgtgt ccctttgccg gaaatattcc
     5461 tgaatggctt cgcaaaatcc ctggtaggga aaggcgccgt cctgctcaat tgatcgccca
     5521 tgcaacacca taagtttgcg ggcattcgcc aggttctcaa tctcgtcgag gagtcgtgtc
     5581 ttgccaactc ccggttcgcc ggaaaccact acgaattggc attcgccgct gacggcggca
     5641 ttgagtcgct tctgcaactc agcttgttcc ttctctctgc caatgaaagg agacggcgcc
     5701 acgcgcgtca gcatcagtgt tttcgtcaat accacggact tgccaagatc gctctggtgc
     5761 agtcgggtct ggcagcgccg taaagaatcg gcgacctcac ttgcgcgttg tgatcttttc
     5821 cccgggtcct tggctatgca gttgagtatg actgcctcaa gatcctcatt gatgtccgcg
     5881 cccattgagc gcggcgactg ggggatctca tgaactatgc gatagagaat ggactggagc
     5941 tcacctgaga atggcggttc gccggtaaga cactcgtata acaccgtacc cagagagtag
     6001 atgtccgaac gatgatcgac accgctggcc gccacttgct ctgggctcat gtaagagagc
     6061 gtgccggcaa tagtccccgt cttggtgatc cgactctcag tggcgccgcg ggcgagacca
     6121 aaatccatta tccgaactct gactctcgca gcatcttcac gcaccaccat gatgttctcc
     6181 ggtttgatat cacggtgaat gacgccgcgt gagtggctgt actcaagcgc ctccgcaact
     6241 tgaatcccga tgtcgagaac ctcgccaagt gtgagggcat tctcgcgttg gaaataccgc
     6301 agatttgtcc cctggacgac gggcataacg aagaacagcg atccctcgtg tttgccaaag
     6361 tcgaaaatcg gcacaaccgc agggtgatcc atctgggcca ccaactgcgc ctcgcgctgg
     6421 aagcgttcct ccatttccgg gctcagcagg gttggaggga taagcttgat ggccacttcg
     6481 cggttcaata agggatcgag tgcccgata
//
LOCUS       DASUEX010000436         3291 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_755259, whole genome shotgun sequence.
ACCESSION   DASUEX010000436 DASUEX010000000
VERSION     DASUEX010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3291
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_755259"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="VFV61_07425"
     CDS             <1..225
                     /locus_tag="VFV61_07425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900348.1"
                     /translation="IGRPGAARRMHQMAGEKFARLGAKIFMSLDLEDYQAQLERALSW
                     LREAADPRELTPPEITPPKIKLLAELTPFD"
     gene            260..754
                     /locus_tag="VFV61_07430"
     CDS             260..754
                     /locus_tag="VFV61_07430"
                     /inference="COORDINATES: protein motif:HMM:NF025046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HEX4900349.1"
                     /translation="MTINYLRLILLAMWLGAAVFFSVVVAPAAFSVLSHMHSMNGRES
                     AGAIVSLCLALINLSGFIISLLTLITGVFRFRKTIWSVVEAASLLIMAVATGVGHWII
                     RAQLQALKSSFAVPIDLIPQDDPRRISFDTLHGYSVKALGVAMIAALIGIAIITYRLH
                     RNAK"
     gene            785..1600
                     /locus_tag="VFV61_07435"
     CDS             785..1600
                     /locus_tag="VFV61_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900350.1"
                     /translation="MEIIEKTDEWRKRYADWQKGHDAAKSNGDVGESYPFVVNRRAPF
                     TPARRALPMLNLALISSAGAYLDGTQSFNLNDPAGDVSFREIPIEVGPDDLKFAARGY
                     DPVAIQADLNTQLPLQRLAEFVANRIIGQLNPVFWSFCGFIPDAGSVVDEMLPLLQER
                     VRRYEVQAALLIPASRLCHQSVSLIARGLEMSGLPTMTIAVAQDVVEAVRPPRAACYR
                     GELGSVAGKANWPEHQRRVLDEALRLIEPMDQPGIRQLTVALQSQVEGERGER"
     gene            1646..2719
                     /locus_tag="VFV61_07440"
     CDS             1646..2719
                     /locus_tag="VFV61_07440"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX4900351.1"
                     /translation="MSLSKRLVPLGVLLIVCALAYLAFWPVPIEPAAWTPPAAPSLTG
                     TYQQNDRLAGTERLSLGPGFAPEDVAIDSEQRIYGGFDDGRIIRLQRDGRRPELFADT
                     KGRPLGLKFDGAGNLIVADAVKGLLSVDHAEVISVLSTAADGIPLRVTNDLDVALNGT
                     IYFTDASSRFPLTNYKADILEHQATGRLLAYEPATKTTRLVLGGLSFANGVAVSPDQS
                     FVLVNETGKYRIHRVWLNGANAGKSEIFIDNLPGFPDGISSNGKDKFWLALVTPRDAT
                     LDKLLPHPFLRKAVWRLPPFLRPAPKRYSFVLGLETNGRVVENLQNGASDCYAQIANV
                     VEHDGFLYFGSIGEATVGRWKIR"
BASE COUNT          736 a          903 c          890 g          762 t
ORIGIN      
        1 attggcaggc ccggcgcagc gcgcagaatg catcagatgg caggtgagaa gttcgcacgc
       61 ctgggggcca aaattttcat gtctttggat ttggaagact atcaggcaca actcgaacgt
      121 gcgttatcgt ggcttcgtga ggcagcggat ccacgtgagc taacgcctcc ggagattacg
      181 ccacctaaga ttaaactgct cgccgaactt acaccattcg attgatcatc tgtactgaga
      241 cccgaagatc cgaccgaaca tgaccattaa ctacctacgt ttgatcttgc tcgcaatgtg
      301 gctgggcgca gcagtatttt tcagcgtggt tgtcgcgcct gcagcctttt ctgtcttaag
      361 tcacatgcat tcaatgaacg gcagagagtc cgcaggcgcc atagtcagtc tctgtctcgc
      421 cctaataaat cttagcggtt ttatcatcag tttgttgacg ctaataaccg gagtattccg
      481 cttccgcaaa accatctggt ctgttgtcga agcggcctcg ctgctgataa tggcagtcgc
      541 aaccggtgtt ggtcactgga taataagagc tcagctgcaa gcgttgaaga gtagtttcgc
      601 ggtaccgatc gacctgattc ctcaagatga tccccgaaga atttcattcg ataccctcca
      661 cggttattcc gtcaaagccc tgggtgttgc tatgattgcg gctctcatcg gaatagctat
      721 tatcacgtat cgtttgcacc ggaatgcgaa atgactcgta tctttgctgc aggaagggac
      781 taccgtggaa attattgaaa aaacagatga gtggcgcaag cgttatgctg attggcagaa
      841 ggggcacgac gccgctaaaa gcaatggtga cgtaggcgaa tcctacccgt tcgttgttaa
      901 tagacgagcc cctttcacgc cggcgcggcg cgccttacca atgcttaatc tggcgttgat
      961 atcttcagcc ggggcctacc tggacggcac tcaatccttc aatctaaatg atcccgccgg
     1021 cgatgtaagt tttcgtgaga tacccatcga agttggaccc gacgacctga agttcgccgc
     1081 gcggggatat gatcccgttg ctatccaggc tgatctaaat actcagcttc ctttgcagcg
     1141 gctagccgaa tttgtggcga accgaatcat cggccaactc aatccggtct tctggagttt
     1201 ttgcggattc attccggacg ccggcagtgt ggtcgatgaa atgctgccgc tacttcagga
     1261 acgcgtgcgg cgctatgaag tacaggcagc gttgctgatt ccggcctcac gcttgtgcca
     1321 ccaatccgtc tctctgattg cgcgcgggct agaaatgagt ggcttgccga caatgaccat
     1381 tgcagtcgct caggacgtag ttgaagctgt gcgaccgccg cgtgctgctt gctaccgggg
     1441 cgagctggga agtgtcgccg gaaaggcaaa ctggcctgaa catcaacgtc gcgtgctcga
     1501 tgaggcgctg cgactcattg agcctatgga tcaacccggg attcgccaac tgacggtagc
     1561 attgcaaagc caggtggaag gcgagcgcgg cgaacgctaa ccagattcac tcacagagct
     1621 ttctaagatt aaggctcggt aattcatgtc tctaagcaaa cggttagtgc cgttgggcgt
     1681 gctcttgata gtctgcgccc tcgcttacct cgctttctgg ccggttccca tcgagcctgc
     1741 ggcctggact cctcccgcag caccttctct caccggcaca tatcaacaaa acgatcggct
     1801 tgccggcacg gaaaggcttt ccttgggtcc tggtttcgct ccggaagacg tggccatcga
     1861 ttcagagcag cgcatctacg gaggcttcga cgacgggcgc atcatccgtc tgcagagaga
     1921 tggcaggcga cctgagttgt ttgctgatac gaaggggcgg ccgctgggtc tgaagtttga
     1981 cggcgcgggc aatctcatcg ttgctgacgc tgtcaaggga ctgctgtcag ttgatcacgc
     2041 cgaggtgatt tctgttttga gcacggcagc cgacggcatc cctctgcgcg ttaccaatga
     2101 tcttgatgtc gcgttgaacg gcacaatcta cttcacggat gcgtcgagca ggttcccgct
     2161 cacgaattac aaggcagaca tcctggagca ccaagcaacg ggccgcttgc tcgcctatga
     2221 accggcgacg aagacaactc ggctcgtgct cggggggcta agttttgcga atggagtcgc
     2281 ggtgagtccg gatcagtcgt tcgtcctggt taacgaaacc ggcaagtaca ggattcatcg
     2341 cgtctggttg aacggcgcga atgccggcaa gtcagaaatc ttcatcgaca acctgcccgg
     2401 atttcccgat ggcatttcgt cgaatggcaa ggacaagttt tggctggcgc tcgtgacacc
     2461 tcgcgatgcc acgctcgaca aactcctgcc gcatccgttt ttgcgaaagg cggtgtggcg
     2521 cttgccgccg ttcctgcgac cggctccaaa gcgctattcc ttcgtcctcg gtttggaaac
     2581 aaacgggcga gtagtggaaa acctgcaaaa cggcgcgagc gattgctacg cgcaaatcgc
     2641 caatgttgta gagcacgacg gctttctcta ctttggcagc atcggtgagg caaccgttgg
     2701 gcgctggaag attcggtaag aaccgcacga aggggcatag cccgctttct aatctgacgc
     2761 catggcgcgg cagagccggc gccgcacatc cggcggcgaa gccgaacgaa cggtccccac
     2821 taatctcagt ttgaggcaat gctgccgatc gatagatcga taaatcgggc tttgccctgg
     2881 aagtagattt atgggaagcc gaaagatcga aagatcggtt acctgctaca cagctgtata
     2941 cctcattacc attcctctcc accctgattc gccaacaaat ctctagttct cacctctcag
     3001 ctcgatttat ggatagctgg tatccaagac cgtttatccg cacgcaaatt ctctttcagc
     3061 gtaagttgtt acgagacgta agcttaggtc tgttacgcga gatcttgcac ttgtggcaca
     3121 ggcattgaag aagaaacagg cgaacgcaag caaggaatct cagtcacccc agggagaaac
     3181 catgatcaac ttactctcag cagctaccgc caccccccag cattgcctgc aaacaaccga
     3241 cctcgtctcg gccctgatgc ataaggtttc tcccgagctg atcaattcca t
//
LOCUS       DASUEX010000437         3112 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1326077, whole genome shotgun sequence.
ACCESSION   DASUEX010000437 DASUEX010000000
VERSION     DASUEX010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1326077"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            28..2088
                     /locus_tag="VFV61_07445"
     CDS             28..2088
                     /locus_tag="VFV61_07445"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX4900352.1"
                     /translation="MRLNQKLPFQPFRPSNSPLPNRPSFKQLSISLILALLATFLCAA
                     LWKLARLVSFDNAFLVHGASHTMTPFPGWALIASITFLSLGTSFAVELAGARRVVPYA
                     TGVLLMLAVCSLGVSRLFAVDIQFTFLLIAVVGSAALVQSRRLWLRERALGGQLIRSV
                     KKLSSLEGADARFRLMSGLKLIQLVLSTNEAVVFRRDSNSQLVSAARLRASQSGPLDP
                     SRNSSWREGIALCERAIKTDSIIVLPASTSSPLTIVAIPLSHEDRSVGALLVRLNQGF
                     EENDRALLSAVGAQMARNLQREEVCKAAAPHWNLSVLSTEAADKKFESLYALNGLISE
                     QRFGIEILSEISDGLAIAYLDGTIAFANANFLRYADLEASVIRSSDLFALLDKLRTDV
                     FDEPSIAVRRVLQAGEPYERELDFSDRNATLGLRISLVKSTAKGEEGEPLCLAVHVTD
                     LTGSKEYEKLKSDMISLMSHELRTPLTSINGFAELLSADEELPEQAKEFVTIIANESQ
                     RLSRMINTFLAVTQLQRKDKQEVLKIPLRLDEIAREIVIGMQPVARKRRIRLVEQQQA
                     QRLPPVAADKSLITQAVKNLVTNAIKYSPEKTTVTVSTALEAEAVRVSVEDRGYGIPA
                     EARDRVWEKFYRVIREGQEKDEESTGLGLAFVREVIEQHGGQVELETEEGRGSKFSFT
                     LPRL"
     gene            complement(2102..2521)
                     /locus_tag="VFV61_07450"
     CDS             complement(2102..2521)
                     /locus_tag="VFV61_07450"
                     /inference="COORDINATES: protein motif:HMM:NF013052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HEX4900353.1"
                     /translation="MKIKLARGLEIKASSIEGKGCFATTNFTHRRKIAEYAGERITNA
                     EANRRAQRRKLRICAINNRWSLDGSRGGNGTHYINHSCQPNAFMKILYSHILFIALRD
                     ISPGEEITIDYESTLHSDKKRCTCGAPLCRGTINKVR"
     gene            complement(2720..>3112)
                     /locus_tag="VFV61_07455"
     CDS             complement(2720..>3112)
                     /locus_tag="VFV61_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900354.1"
                     /translation="TAWRMIAEPDAAHEHLEFVYGDRLQGYLRIRKEVVEAGTSASDL
                     ARRELEQKLRFVPGFVEGKEEKFAGRLSGVTVSYEYVNTGKAMLGRTYYLQVDNRTIY
                     ALRFSGLRDQLTRIRNQTDLIARSFKLK"
BASE COUNT          730 a          824 c          830 g          728 t
ORIGIN      
        1 tccggctgtg tataatcgct tcaggctttg cgcctgaacc aaaagcttcc gtttcaaccc
       61 ttccggccct ccaactcgcc cttgccgaat cgaccttcct tcaaacagct ctcgatctct
      121 ttaatactgg ctttgctggc gacatttctg tgtgcggcgc tttggaagct tgctcggctg
      181 gtctcctttg ataatgcctt tctggtgcac ggcgcatctc acacgatgac acccttccct
      241 gggtgggccc tgatcgcatc aatcacgttc ctcagtctcg gaacctcatt cgcagtggag
      301 cttgccggag cccggcgtgt tgtcccctat gctaccggcg tgcttctcat gcttgcagtc
      361 tgtagtttgg gtgtttcgcg actatttgca gtcgacatcc aattcacctt tttgctgatc
      421 gccgtcgttg gttctgcggc cctggtccaa agcaggagat tatggttgcg cgagagagcg
      481 ctaggcggac aactgatccg ctcagtgaag aagttgagct cactggaagg ggcggatgcg
      541 aggttccgcc tgatgagcgg gctaaaattg attcagctag ttcttagcac gaatgaagcg
      601 gtagtttttc gtcgtgacag taacagccaa ttggtttccg cagcgcggct tcgtgcatca
      661 cagagtggac cgcttgatcc cagtcgaaac tccagctggc gcgagggcat cgctctctgc
      721 gaacgcgcaa tcaaaactga ttcgatcatt gtcttgccgg cttcgaccag ctcgccccta
      781 acaattgtgg ccataccact gagtcacgag gatcgctccg tgggagcatt gctggttcga
      841 cttaaccagg gttttgagga gaatgatcgc gcactacttt cggcagtcgg cgcacagatg
      901 gcgagaaacc tgcaacgcga agaggtgtgc aaagctgccg caccgcattg gaacctcagc
      961 gtattgtcca ccgaggcagc cgataaaaag ttcgaatcac tctatgctct caatgggctc
     1021 atttccgaac aaagatttgg tattgagatt ctttccgaaa tctccgacgg actggccatc
     1081 gcatacctcg acggcactat cgcattcgca aacgcaaact ttctccgtta tgccgacctt
     1141 gaagccagcg taattcgttc ttctgatctg tttgcactgc tggacaagct gcgcactgac
     1201 gtgtttgatg agccctcgat tgcggtgcga cgcgtattac aggcgggcga gccctatgaa
     1261 cgtgaactag atttcagcga tcgcaatgcc acgttgggat tacggatttc acttgttaag
     1321 tcaacagcca agggcgaaga aggtgaacca ctttgtctgg cggttcatgt aaccgatcta
     1381 acgggatcga aagagtatga aaagctgaag agcgacatga tatcgctcat gtcacacgag
     1441 ctgagaacgc cccttaccag cattaatggg ttcgcggaac tcctcagcgc tgacgaggaa
     1501 ctccccgaac aggcaaaaga gtttgtcacg atcattgcca acgagtcaca gcggctgtca
     1561 cgaatgatta atacgttcct ggccgtcacg cagctacagc gcaaagacaa acaggaggtc
     1621 ctcaagatcc cgttgcgact ggatgaaatc gccagagaga tagtcatcgg gatgcaaccg
     1681 gttgcaagga aaaggcgcat caggctcgtc gagcagcagc aggcacagcg cctgccccca
     1741 gttgcagccg acaagagctt gatcacgcaa gcagtgaaga accttgtgac taatgcgatc
     1801 aagtacagtc ccgaaaaaac taccgtaact gtgtccacag ctctcgaagc cgaagcggtg
     1861 agagtttctg tcgaagatcg cggttacgga ataccggctg aggccaggga tcgagtttgg
     1921 gagaagttct atcgggtgat acgcgaagga caggaaaagg acgaggagtc caccggtctc
     1981 ggattggcct ttgtacgcga agtaattgaa caacacggtg gccaggtcga actggaaacg
     2041 gaggaaggtc gaggctctaa attcagtttt acgttaccga gattatgagg ttcatcgaag
     2101 gttaacggac tttattaatc gtcccgcggc aaagaggtgc accacaagtg cacctctttt
     2161 tatctgagtg cagggtcgat tcgtagtcaa tcgtgatctc ttcgcccggg ctgatatcgc
     2221 gcagggcgat aaagaggatg tgactgtaca ggatcttcat gaacgcattt ggttggcagg
     2281 agtgatttat gtaatgagtc ccattgccgc ctctgctgcc atccaaactc cagcgattat
     2341 ttatcgcgca gatccgcagc ttgcgacgct gagcacgccg gttcgcctca gcgttagtaa
     2401 tacgttcgcc cgcatactcg gctatcttcc tgcggtgggt gaagttggtt gtcgcgaaac
     2461 aacccttgcc ttcaatagaa gatgctttga tctcaagacc gcgcgccagc ttaatcttca
     2521 tgttcgctgc gagccatgat acaggacgct gcaagttcac aagcgacgaa cgcctaactg
     2581 ctcaggggca gaaaggttat taacctggtg acgacacccg ttgagcaaga aaaagccggc
     2641 gttcgatgat gccggcagtg ttgaatcggt aaagcgcaag ctcaaggcct gcgaatgaac
     2701 gcgaggtctg cgaagttaac tatttcagct tgaaacttct ggcgatcaaa tcagtctggt
     2761 tgcgaattcg ggttaactgg tcgcgcagac cggaaaagcg cagagcatag atggtccgat
     2821 tgtccacctg cagataatag gtgcggccaa gcattgcttt gccggtattt acatactcgt
     2881 aagagacggt gacaccgctt agccgtccgg caaacttctc ctctttcccc tcaacaaagc
     2941 cgggcacaaa tctcagcttc tgttctaatt cccggcgtgc caggtctgac gcagacgttc
     3001 ctgcctctac gacctctttg cgaatccgaa ggtagccctg caaacgatcg ccataaacga
     3061 attccagatg ctcgtgggcg gcatccggtt ccgcaatcat tcgccaggcc gt
//
LOCUS       DASUEX010000438         2536 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_84799, whole genome shotgun sequence.
ACCESSION   DASUEX010000438 DASUEX010000000
VERSION     DASUEX010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84799"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            180..264
                     /locus_tag="VFV61_07460"
     tRNA            180..264
                     /locus_tag="VFV61_07460"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:214..216,aa:Leu,seq:tag)
     gene            482..1837
                     /gene="tig"
                     /locus_tag="VFV61_07465"
     CDS             482..1837
                     /gene="tig"
                     /locus_tag="VFV61_07465"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEX4900355.1"
                     /translation="MKTELLDVSPTRKEIKIEIEPETVRATYDRVSDTYARQVNVPGF
                     RKGHAPRSVVRTRFKNEIRGDVLRELVPDAVNEAIGKHELAAIGEPDVHLDNEETLDQ
                     LGQGPIAVHVNVEILPTVELNRYKGLAATRRVRPVTDADVDRLIDGLREASASLQPVE
                     DRGAELGDTLTVTFHGKFLDSPETEDINVTDVDVVLGGERVQPEFTENLLGAHVDEQR
                     NFTVNYPEDFSSEGLAGKKVGYTATVTAVRLKELPEVDDEWAQSLGDDFDSVATLRAK
                     VREDLEKRSGTEADHRLRSELMKSLLTAHPFEVPQSLINHQTNYRMESVVREMIGKGI
                     DPRSQQLNWEGAREELQAQAEEDVRGSLLLERIAEEEQIEVSTEEIEAEIDAIATATR
                     QPKEQVRAVLTKDGGERSIANRLRNRKALDLLLENAIITDEEWREDEDVEQAQDVKNK
                     E"
     gene            1909..2493
                     /gene="clpP"
                     /locus_tag="VFV61_07470"
     CDS             1909..2493
                     /gene="clpP"
                     /locus_tag="VFV61_07470"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEX4900356.1"
                     /translation="MALVPMVVEQTSRGERAFDIYSRLLKDNIIFLGTPIDDQIANLI
                     VAQLLFLEAEDPEKDINLYINSPGGSITAGMAVYDTMQFIRPDVTTICVGQCASMGAL
                     LLAAGAKGKRFALPNSRILIHQPTGGSQGQATDIRIQAEEILRMRELTSQILAKHTGQ
                     SYETVERDVERDKIMSAMQAKEYGLIDEVIAHRE"
BASE COUNT          660 a          635 c          702 g          539 t
ORIGIN      
        1 cgactcacgt ccggttgata ttccggaacc tcgagcggcc ggagggatcc gaggagttaa
       61 ttcaaagacc aaagacccaa gtccaaagac ccacggacaa ctgattgtac cccctgtgac
      121 gaaagcaact tcgccgtgat attctttttt gttattgatg agctgatttt tgttaccgtg
      181 cgaaagtggc ggaattggca gacgcgccag acttaggatc tggtaccgaa aggtgtgggg
      241 gttcgagtcc cccctttcgc accacgaaca gtggatagtg ggcagtgggc aggttgtgat
      301 cttgtgtctt tggtctttgg tcttgggcct ttgatcttgg ctctttggaa gttgactccc
      361 ttaataagct acccgaacag ctctcaggga tcgtaaccaa gcgaccaaag cccaaagccc
      421 aaagcccaaa gcccaaagac ccaactggca actgacaact gaaaatcgac tacagattat
      481 gatgaagact gaattacttg atgtctcccc aacgcggaag gagataaaaa ttgaaatcga
      541 accggaaacg gtgcgcgcaa cctatgaccg tgtgagcgat acctacgcac gccaggtcaa
      601 cgtacccggc ttccgcaaag gacatgcacc cagatcagtg gtgcgcaccc gcttcaagaa
      661 cgagatccgg ggagacgtgt tgcgcgaact ggttcccgat gcagtgaatg aagctatcgg
      721 caagcatgaa ctggcggcta tcggagagcc ggatgtgcat ctcgacaatg aggaaacgct
      781 ggaccagttg ggccaggggc cgatcgctgt tcacgtgaat gtcgagattt tgccgacggt
      841 agaactcaac aggtataaag gactcgcagc cacgcgccgg gtgcggccgg tgactgatgc
      901 ggacgtggat cgtctaatcg acgggctgcg tgaggcgtct gcatcgctac aacctgtcga
      961 agatcgcggc gcggagttag gtgacaccct gacagtcact tttcacggaa agtttcttga
     1021 ctcacccgaa actgaagata tcaatgtcac agacgttgat gttgtcctcg gcggcgaacg
     1081 tgttcagcct gagtttacag agaacctgct gggcgcccac gtcgatgaac agaggaattt
     1141 taccgtcaac tatccggaag atttttcctc tgaaggattg gccgggaaga aagtgggcta
     1201 cacagccacg gtgacggctg tgcgacttaa ggaactcccg gaagtggatg acgaatgggc
     1261 acaaagtctg ggcgatgatt tcgactcggt ggcaactctt cgagccaagg ttcgcgaaga
     1321 tttggaaaaa cgctccggca cggaagctga tcaccgcctt cgctctgaat tgatgaagtc
     1381 actactcact gcgcatccat ttgaagtgcc gcagagcctc atcaatcatc aaaccaacta
     1441 ccgcatggag agtgtggtgc gtgaaatgat cggcaaaggg atcgatccgc gcagtcagca
     1501 gttgaattgg gaaggcgcgc gcgaagaatt gcaggcccag gcagaagaag atgttcgcgg
     1561 ctcgttactg ctcgaacgta tcgccgagga ggagcagatc gaggtgtcaa ctgaagagat
     1621 cgaagcagag attgatgcga ttgcaactgc tacgcgacaa ccaaaagaac aagtgcgtgc
     1681 cgtcttgaca aaggatgggg gcgaacgtag catcgcgaac aggttacgta atcgtaaagc
     1741 gttggacttg ttgcttgaaa atgcaatcat caccgatgaa gagtggcggg aagatgagga
     1801 cgttgagcaa gctcaggacg tcaagaacaa agagtagtcg taactagaaa ttttgaattc
     1861 cttccaccca atcatcattc agctttccat agacgaggtt aatttcttat ggctttagtc
     1921 cctatggtcg ttgagcaaac gtcgcgtggc gaacgggcgt ttgatattta ctcgcgactg
     1981 ctgaaagaca acatcatttt cctcgggacg ccgattgacg atcagattgc caatctgatc
     2041 gtcgcgcaac ttctctttct cgaagccgag gatccggaaa aagacatcaa cctgtacatc
     2101 aattcacctg gtggctccat aaccgccggc atggccgtct atgacaccat gcagttcatt
     2161 cgtcctgacg tgacaacgat ctgcgtcggc cagtgcgcgt caatgggcgc actgctgctg
     2221 gctgccggtg ccaagggcaa gcgctttgcc ttgccaaact cccgcatcct cattcaccaa
     2281 ccgactggtg gctcgcaggg acaggcaacc gatatccgca ttcaagccga ggagatcctg
     2341 cgaatgcgtg agttgacgtc ccaaatcctg gcaaaacaca ccggccagtc ctatgaaacc
     2401 gtagagcgtg atgttgaacg cgataaaata atgtctgcca tgcaggccaa agaatatggt
     2461 ttaatagacg aggtgattgc gcatcgagaa tgatgcgttc ggccctcacg gaggcactca
     2521 tgatcgccaa gatgaa
//
LOCUS       DASUEX010000439         2665 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1661655, whole genome shotgun sequence.
ACCESSION   DASUEX010000439 DASUEX010000000
VERSION     DASUEX010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1661655"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1225
                     /locus_tag="VFV61_07475"
     CDS             <1..1225
                     /locus_tag="VFV61_07475"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX4900357.1"
                     /translation="TADATRLSEILGAPNPGAAITITPKSITEIVEVMKRASESGSSV
                     VPAGAQTWLKSSNQRNGNLVISTLGLNRIVEHEPADLVATAEAGVSLSTFNRELARNG
                     QWLPLDPPDNGAATLGGVVATGLAGPRQFGYGPPRRYVIGMRVVLADGTQIKVGGRVV
                     KNVAGYDLCKLFTGSYGTLGIISEITFKLRPVPETSATLLATGTLTSLLKAGQALMTS
                     ALFPVAIEILSAAIVAELELNGTAEHALLIQFAGTQAGVAQQVERAVSTIRRDFATAN
                     VMTLEAPSSVWRSIAASETGHEQLSWRGGIQPGKLAEVVCHLTGRWNAGVGSGIVSGD
                     FEGNLEEAIRLQQRMRSLGGWLTIQNSPAELDRIGDEGDPGIMRLMQRIKSELDPRGV
                     FPGLGRQASRLPQSP"
     gene            1294..>2665
                     /gene="ggt"
                     /locus_tag="VFV61_07480"
     CDS             1294..>2665
                     /gene="ggt"
                     /locus_tag="VFV61_07480"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX4900358.1"
                     /translation="MKDMKTHPLLRLLLAVLLTASFVSVPTADFLSLRAPSAAVAASR
                     EPVRAKNGIVASTNEVASRIGVDILKQGGNAVDAAIAVAFALAVTHPSAGNLGGGGFM
                     MIRMRDGKTVAIDYREMAPASATRNIYLDKQGNPIEGEGGSLVGYRASGVPGTVRGME
                     LALKKYGSRRLTWAQLVEPARRLAANGLVVNYSLARSLRGNQDYLSQYPETKRIYLRN
                     GNFYQEGEIFRQPELAQTFARLQKGGPNEFYTGETARLIVADMQRNNGLITMDDMRAY
                     VAKEREPLSGNYRGHEIISMPPPSSGGAVLVEMLNILEGYDLSKMDWASSDRYHLMTE
                     AMRRAFADRAEYMGDTDFVKVPIAGLIDKNYAAQLRSSINLARASSSETVRAGKPAGH
                     ESDETTHFTVVDAQGNAVANTYTLNNSYGSAVVAKGTGIIMNDEMDDFAAKPGTPNLY
                     GLIQGER"
BASE COUNT          598 a          716 c          803 g          548 t
ORIGIN      
        1 aacggcggat gccactcgac taagtgaaat cctcggagcg cccaatcccg gcgcagcgat
       61 cacgatcaca ccaaagtcca taactgaaat cgttgaagtg atgaaacgcg cttcagagtc
      121 aggctcgtcg gtggttccgg cgggcgccca gacgtggctg aagagcagca atcaacgcaa
      181 cgggaacctg gtcatcagca cactcgggtt gaatcgcatc gtcgaacatg agcccgccga
      241 tctggttgcc acggcggaag ccggggtctc gctgagtact ttcaatcgtg agttggcgcg
      301 aaacggccag tggttaccgc ttgatccgcc cgataatgga gcggcaacac tgggtggagt
      361 ggtggccaca ggtcttgccg ggccgagaca attcggttat gggccaccgc gccgttatgt
      421 gattggcatg agggtcgtcc tggctgatgg cacgcagatt aaagttggcg gccgagtggt
      481 gaaaaacgta gctgggtatg atctctgcaa gcttttcaca ggcagctacg gtacgttggg
      541 aatcatcagc gaaatcactt tcaagctccg gccagttcct gaaacgtcag ccacgctcct
      601 ggccaccgga acgctcacct cgttgctcaa ggccggtcaa gcgttgatga cctcagcact
      661 gtttcccgtt gcgattgaga ttctttcggc ggcgatcgtt gcggagttag agctcaacgg
      721 cacggcggaa cacgctctgc tgatacaatt tgcgggcacg caagccggag ttgcccagca
      781 ggtcgagcga gccgtgagta ccataagacg agactttgct actgcgaatg ttatgactct
      841 cgaagcgcct tcgagtgtgt ggcgtagtat cgccgccagc gagaccggcc atgagcagct
      901 gagttggcgc ggaggaattc agcccggcaa actggctgag gttgtctgcc atctgacagg
      961 ccgctggaac gccggcgtgg gcagcggcat cgtgagcggc gactttgaag gcaatctcga
     1021 agaagcgata aggcttcagc aacgcatgcg aagtctgggt ggctggctca caattcagaa
     1081 ctcgccagcg gaacttgatc gaataggtga tgagggcgat ccgggcataa tgaggttgat
     1141 gcagcgcatc aagagcgaac tcgatccgcg cggtgttttt cccgggctgg gaaggcaggc
     1201 gtctcgccta ccgcaatcac cgtaaggtga gttaagtgta aaggacttct gctaatcggt
     1261 tgttcgcgct gtgcgaccct ggagttcacc atgatgaaag acatgaaaac gcacccactg
     1321 ctcaggctgc ttctcgcagt tctgctgacc gcgtcgttcg tctcagttcc aactgcggat
     1381 ttcttatcgc tgcgtgctcc ctcagcggcg gttgctgcat cgcgcgaacc ggtccgggcc
     1441 aagaacggca tcgttgcgtc aacgaatgag gttgcctctc gcatcggggt ggacatcctc
     1501 aagcagggcg ggaatgccgt cgatgcggca atcgcagtcg cttttgcgct ggccgtaaca
     1561 cacccgtcgg ccggcaatct cggcggtggc ggcttcatga tgattcgaat gcgagatgga
     1621 aaaacagttg ccattgacta tcgagagatg gcgcccgcgt ctgcgacgcg caatatctat
     1681 cttgataagc aaggcaatcc gatcgaaggc gagggcggtt ccctggttgg ctatcgtgcc
     1741 tccggcgttc ccggaaccgt gcgcggcatg gaattggcgc taaagaaata tggctcacga
     1801 cgcctcacct gggcgcaact ggtggagccc gcacgtcgcc tcgcagcaaa tggcttagtt
     1861 gtcaattaca gcttggcgcg gtcccttcga ggcaatcagg actacctctc tcaatatccg
     1921 gaaacgaaac gaatctattt gcgcaatggg aacttttatc aggagggcga gatttttcgc
     1981 caaccggagc tcgcccaaac ctttgcgcga ctgcaaaaag gcgggcccaa cgagttttat
     2041 acgggagaga ctgcacggtt gattgtggcg gacatgcaac gaaacaacgg actcatcacg
     2101 atggatgaca tgcgagccta cgtcgccaaa gagcgagagc cgctcagcgg aaactatcgt
     2161 ggtcatgaaa tcatctccat gccgccgcct tcgtcgggtg gagctgtgct ggtggagatg
     2221 ctcaacatcc tggaaggtta tgacctgagc aagatggact gggcctcgtc tgatcgatat
     2281 cacttgatga ctgaggcgat gcgtcgcgcc tttgccgatc gtgcggagta catgggtgat
     2341 acggatttcg tgaaagtacc gattgcgggt ttgatcgaca agaactacgc ggcgcaacta
     2401 cgctcaagca tcaatctcgc acgggcttca tccagtgaaa cagtgcgcgc cggtaagccc
     2461 gcgggccatg agtcagatga aaccacacac tttacggttg tagatgcaca gggcaacgca
     2521 gtcgctaaca cctatacgct gaataattcg tacggctcag ctgtcgtcgc gaagggtacc
     2581 ggcatcatca tgaacgatga gatggacgac tttgccgcca aaccgggcac gcccaatcta
     2641 tatggtctga ttcaaggcga gcgaa
//
LOCUS       DASUEX010000440         2944 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_755793, whole genome shotgun sequence.
ACCESSION   DASUEX010000440 DASUEX010000000
VERSION     DASUEX010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_755793"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1719)
                     /gene="lon"
                     /locus_tag="VFV61_07485"
     CDS             complement(<1..1719)
                     /gene="lon"
                     /locus_tag="VFV61_07485"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEX4900359.1"
                     /translation="MEEYSDHTPADIARYPMVPIRDVVIFPFTKVAFKIGRPGSVRAL
                     ELALASDRTIFLSTQHDATVDEPSPDQIYRVGTIARILQSQRQENGQIKVVVEGRERA
                     TTIRVENNDGAFTALVRRAPVVSEEGVRTDALVQKVAQLIEQHLRLAPDVQPDALQTA
                     MRNVDASHMSDALASQLRISVEDKQGLLELFSTQARLARLIEILEIELEKRQLDRTIQ
                     TRVKRQMEKAQKEYYLNEQIKAIHKELGRKDERAELDELKKKIEEAGMTEEAKEKALQ
                     ELHRLEAMPPMSAEGTVSRTYIDWLVSVPWKQKSKEIKDLTKAEEVLNDDHYGLEKIK
                     DRILEYLAVRQLVKNPKGSILCFVGAPGVGKTSLGKSIARATGRKFVRLSLGGVRDEA
                     EIRGHRRTYIGALPGQIIQMMKKAGTVNPVLLLDEVDKLGTDFRGDPSAALLEVLDPE
                     QNHMFQDHYLDVEYDLSKVFFIATANVLHTIPPALQDRLEVLRLSGYTEREKLEIAKR
                     HLIPKQAEANGLRTEQFEFTDDGILEIIRHYTRESGVRNLEREIGSCCRKIARKFVGE
                     NVAPDFK"
     gene            complement(1940..>2944)
                     /locus_tag="VFV61_07490"
     CDS             complement(1940..>2944)
                     /locus_tag="VFV61_07490"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX4900360.1"
                     /translation="RNFESLENENARLMQDANIEHNMVGESTLLQRVYQLISKVAPTD
                     STVLISGESGTGKELVARAIHRSSKRADKPFIAVSCAALAETLLESELFGHEKGAFTG
                     ALIQKKGRLELADSGTIFLDEIGDLSLSMQTKLLRVLQEREFERVGGTQTVKIDVRLL
                     AATNRNLDEAITRGTFRQDLYFRLNVVRLKMPPLRDRREDIPLLANYFARKYAEKCAR
                     KILGISPEARRLLESYDWPGNVRELENAIERAVVLGSTDYILADDLPEPVLEHDAPSS
                     GEGSASYHEQVVQMKKQMIIDAMKQCHGSYTAAAKLLHLHPNYLHRLIRNLNLKDQLR
                     "
BASE COUNT          598 a          847 c          775 g          724 t
ORIGIN      
        1 cttgaaatcg ggcgccacat tttcgcccac gaatttccgc gcgatcttgc ggcagcacga
       61 gccgatttca cgctccaggt tgcgaactcc cgattcacgc gtgtaatggc gaatgatttc
      121 gaggatgccg tcgtcggtaa attcaaactg ttcagtgcgc agaccgtttg cttccgcttg
      181 cttcggaatc aagtgacgtt tggcaatctc gagtttctcg cgctcggtat acccggacag
      241 gcgcagcact tccagacgat cctgcaacgc cggcggaatc gtgtgcagca cgttcgcggt
      301 ggcaatgaag aaaactttcg agagatcgta ttcaacgtca agatagtgat cctggaacat
      361 gtggttctgt tccggatcga gaacttcgag taaagccgca ctcgggtcgc cgcggaaatc
      421 cgttcccaac ttgtcgactt cgtcgagcag caggacggga ttgacggtgc ccgccttttt
      481 catcatctga atgatctgac cgggaagcgc accgatatag gtgcgacgat ggccacgaat
      541 ctcggcttcg tcacgcacgc cgccgagcga cagccgcacg aatttgcgcc ccgtcgcgcg
      601 ggcgatcgat ttcccgaggc ttgtcttgcc gactcccggc gcgccgacga aacaaaggat
      661 cgagcctttc ggatttttta ctaattgccg cacggcgaga tattcgagga tgcgatcctt
      721 gatcttctca agcccatagt ggtcatcgtt aagcacctct tcggccttcg tcaggtcctt
      781 gatctccttt gacttctgtt tccacggcac gctgaccagc cagtcgatat acgtccgcga
      841 aacggtgccc tcagccgaca tcggcggcat cgcctccagc cggtgcaatt cctgcagcgc
      901 cttctccttg gcctcttcag tcatgccggc ttcttcgatt ttcttcttca gttcatcaag
      961 ctctgcccgc tcgtctttgc gacccaattc tttgtgaatg gctttgattt gctcgttgag
     1021 gtaatactcc ttttgcgcct tttccatttg acgcttaacg cgtgtttgga tcgtgcgatc
     1081 gagttgccgt ttctccagct caatttcgag aatctcgatc aagcgcgcga gccgtgcctg
     1141 cgtggaaaac aattccagaa gtccttgttt gtcttcaact gagattcgta gttgcgaggc
     1201 gagtgcatct gacatatgcg acgcgtcgac attccgcatc gccgtttgca gagcatccgg
     1261 ttggacatcg ggagccaggc gcaaatgctg ctcgatcagt tgcgccacct tctgcactaa
     1321 ggcgtcggtc cgcactcctt cttcgctcac gaccggggcg cggcgcacca gtgcggtgaa
     1381 tgctccatca ttgttctcga cgcgaattgt cgtcgcccgt tcgcggcctt ccacgaccac
     1441 tttgatttgg ccgttctcct ggcgttgcga ttgcagaatt cgtgcgatcg tgcccacacg
     1501 ataaatctgg tcgggtgaag gttcatcaac cgtcgcgtcg tgctgcgtcg acagaaagat
     1561 cgtgcggtcg gacgccaggg ccaactcgag cgcgcgcact gagcccggac ggccgatctt
     1621 gaaggcaact ttggtgaacg gaaaaatcac gacatcgcga atcggcacca tcgggtaacg
     1681 ggcgatgtca gcaggggtgt ggtcagaata ttcttccatg attgcttact gcggaatgaa
     1741 cgaaaactct tgtagatttt gcgccagaaa catgaaataa acgaccaatg ccttcgctat
     1801 ctcagatatc gtccaaacgc aaacggtcgc aaggggcgat tttacctttc tctgatcgcc
     1861 cgaggtgctg acggttgatt gtaaactatt tgaaaacgcg ccgccactga gaagttagaa
     1921 gcgcgccggc ttagtggggc tatcggagtt ggtcttttaa attgagattt ctaatcagcc
     1981 ggtgcaggta attcggatgc agatgcagca gtttcgccgc tgccgtgtag ctaccgtggc
     2041 attgtttcat cgcgtcgata atcatctgct tcttcatctg cacgacttgc tcgtgataac
     2101 tcgcgctgcc ttcgcctgaa gacggggcat cgtgctccag caccggctcc gggaggtcgt
     2161 cagcgagaat gtaatcagtc gatcccagca ccaccgcgcg ttcgatcgcg ttttccagtt
     2221 cgcgcacgtt tcccggccag tcataactct cgagcaggcg ccgtgcttcc ggcgagatgc
     2281 ccagaatctt ccgggcacat ttctcggcat actttcgcgc aaagtagttt gccagcaacg
     2341 gaatgtcttc gcgccggtca cgcagcggcg gcatcttcaa acgaacaacg ttgaggcgga
     2401 aataaagatc ctggcgaaaa gttccgcggg taatcgcttc gtccaggttg cgattggtcg
     2461 ccgccagcaa ccggacatcg atttttaccg tttgagtgcc gccgacgcgt tcgaactctc
     2521 gttcctgcaa cacgcgtaaa agtttggttt gcatcgaaag tgagaggtcg ccaatttcat
     2581 caaggaaaat tgtgccgctg tcggccagct cgagccggcc tttcttctga atcaacgcgc
     2641 ccgtaaacgc ccctttctcg tggccgaaca attcgctttc caaaagcgtc tctgccagag
     2701 ccgcgcaact caccgcgatg aaaggcttgt cggcgcgttt gctgctacga tgaattgcgc
     2761 gcgccaccaa ctccttacct gtgccgctct cgccggaaat caaaaccgtc gagtcagtcg
     2821 gcgcaacttt tgaaatcaat tggtaaacac gctgtagcag cgtactttcg ccgaccatgt
     2881 tgtgttcgat attcgcgtcc tgcatcaggc gcgcgttttc attctctaac gactcaaaat
     2941 tacg
//
LOCUS       DASUEX010000441         2749 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_998998, whole genome shotgun sequence.
ACCESSION   DASUEX010000441 DASUEX010000000
VERSION     DASUEX010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2749
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_998998"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            200..1405
                     /gene="ribB"
                     /locus_tag="VFV61_07495"
     CDS             200..1405
                     /gene="ribB"
                     /locus_tag="VFV61_07495"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HEX4900361.1"
                     /translation="MAFATIEDAAADIREGRMIIIVDDEDRENEGDLVCAAEKTTPEV
                     INFMARHARGLICLPLTEERCDELHLTTQVADNTSFLGTAFTVSIDGRKGVSTGISAS
                     DRATTILTAVDPKTRPSDLARPGHVFPLRARTGGVLVRPGQTEASVDIARLAGLYPAG
                     VICEIMNEDGTMARLPELKEFAALHDLKMITVADLVRYRISKETLVRRVVETDLPTVH
                     GLFRAVAYENIINGDVHLAMVMGELDDTPDEPVLVRVHTENVTCDMFGSLIDDTGYQL
                     HSALEKIAAAGQGVVLYLRQREHSLDLVNQLRTYAVMNEKGKSKQEASLETNYGLDRD
                     YGVGAQILHDLGLRRILLLTNHPPKVAALEGFELEVVGNVPLGQPAYLKEPSAERKAP
                     LTEKREQRT"
     gene            1562..>2749
                     /locus_tag="VFV61_07500"
     CDS             1562..>2749
                     /locus_tag="VFV61_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900362.1"
                     /translation="MSVDDRENHSDNAADEPTRERDEVAKALQSETDEPLPPRAEAIA
                     NRDARRPHPHTPQGRRRRYLTKRNALIATVAASIGVVALILLALLLYRLGYVDNYVAG
                     QIKNTFATYGIRAEIKDFHTSFNPQTVEMLGVELFDAQTGEKLGKIDRVLATVRIEDL
                     YALNLRRNINLQELKIEGLEAWVTFDEQGRSNFRNIHIPPPEPNRRILFAYSTARLEV
                     QNSVVHYGDRRHEISGEARNLHATIQPDDPNAPADSWMNTVTLALSKSTFIYDGRPLN
                     DISVNAKGRVNQTRAEIHELIVRTPIGDAKLDGTMDDWRALRYQMNITSTVDLTQASE
                     ILHPQTTLRGAGNFAGKVSGEGDQYKVDGTIKSDALAADGVRLQGLNVTAAGSGQGKT
                     YNIK"
BASE COUNT          636 a          802 c          767 g          544 t
ORIGIN      
        1 gggggcgtcc ctgcgtgaac gccccttgct gcgaattcgg gacgcccacg gaggggtgcc
       61 ccaacagcgc gttcgaaaaa atcaggtctt tcaggtaaca tgcggggacg gtagttagta
      121 gccagaaggc agtaggccca aggcagtagg cggtagacgc caagacccaa ggcccaagac
      181 caaaagccaa agaccattta tggcattcgc aaccatagaa gacgccgccg ccgacattcg
      241 tgaaggtcgc atgatcatca tcgtcgacga tgaagatcgc gagaatgaag gcgatctcgt
      301 ttgcgctgcc gagaagacta ctccggaagt gatcaacttt atggcgcgcc atgcgcgcgg
      361 ccttatctgt ctcccactca ccgaggagcg ctgcgacgag ttgcacttga ctactcaggt
      421 tgccgacaac acctcatttc tcggtaccgc tttcacagtt tcaatcgatg gtcgcaaagg
      481 tgtttctact ggcatctcag catccgaccg cgccacaacc atcctgactg cggtagatcc
      541 caagaccagg ccgtcagacc tcgcccgtcc cggtcacgtg tttccgttgc gggcgcgcac
      601 cggaggcgtg ttagtgcgac caggtcaaac agaagcgagc gtcgatatcg cgcgtctggc
      661 aggcctctat cccgccggtg tgatttgcga gatcatgaac gaagacggaa ccatggcccg
      721 gttaccggag ttgaaagagt ttgccgcgct gcatgatctg aagatgatta ccgtcgcaga
      781 tctggtgcgc tatcgcatca gcaaagaaac actcgtgcgg cgcgtggtgg aaactgactt
      841 acctactgtg catggactct tccgcgcggt cgcctacgaa aatatcatta acggcgacgt
      901 tcatttagcg atggtgatgg gcgagctgga cgataccccc gacgagcccg tcctggttcg
      961 cgtgcatacc gagaacgtaa cgtgcgacat gttcggttct ctgatcgacg acactggtta
     1021 tcaactacac tcagcgttgg agaaaatcgc agccgcgggt cagggcgttg tgctttacct
     1081 acgccagcgg gaacatagtc ttgatctcgt taaccagttg cgcacttacg ccgtcatgaa
     1141 tgagaaaggc aagagcaaac aggaagcgag tctggaaacc aattacggac tcgaccgtga
     1201 ttacggtgtg ggcgcgcaaa tcctgcacga cctgggcctg cggcgcattc tcctcctgac
     1261 aaatcatccc cccaaagttg cggcgctcga gggctttgaa ctcgaagttg tcggcaatgt
     1321 ccccctgggt cagcccgcct atctgaaaga gccgtcggcg gaacgaaagg cgccgctgac
     1381 cgaaaaaaga gagcaacgta cgtgagctgc gacgtcatct cttagttcga ctcctggtag
     1441 agtttgcttc gcgagttggt tcaccctgac cattgcgttg caagcggctc gtttccagct
     1501 tttcgggctc tccctctata atcctaagat tgttgtttgg cccacttggc ggctgtgcgc
     1561 tatgagtgta gacgatcgcg agaaccattc agacaacgct gccgatgagc caactcgtga
     1621 acgcgacgag gtggcgaagg cgctgcaatc cgaaacggac gaaccattgc cgccgcgggc
     1681 cgaagcaatt gccaaccgcg acgcgcgtcg tccgcacccg cacacgccgc agggaagacg
     1741 tcgccgctac ttgacgaaac gcaacgcatt aattgcgacc gtcgctgcat cgatcggcgt
     1801 agtagccctg atcctgctgg ccctgctcct ctaccggctg ggctacgtcg acaactatgt
     1861 tgccggccaa atcaaaaaca catttgcgac ctatgggatt cgtgccgaga ttaaagactt
     1921 tcacacgtct ttcaatccgc agacggtcga gatgcttggc gtggaactgt ttgacgccca
     1981 aactggcgag aagctcggca agatcgatcg cgtcctggct acagttagaa tcgaggatct
     2041 ctacgcactc aacctgcgcc gcaatatcaa tctccaggag ctgaaaattg aagggctgga
     2101 agcgtgggtc accttcgacg agcagggtcg ctctaacttc cgtaacatcc acatccctcc
     2161 tccggagccg aaccggcgca tcctgtttgc ttattcgacc gcccgcctcg aagttcaaaa
     2221 cagcgtagtt cactacggcg acaggcgaca cgagatctct ggtgaagcgc ggaatcttca
     2281 cgcaactatt caacccgatg atcctaatgc gcccgctgac agctggatga ataccgtcac
     2341 cctcgcgctc tcaaaatcaa cttttattta cgacggacgg ccgcttaacg acatttcggt
     2401 caacgccaaa ggccgcgtga atcagacgcg cgcggaaatt cacgagctga tcgtgcgcac
     2461 gccgattggc gacgccaagc tcgacggcac gatggacgac tggcgggcac tccgttacca
     2521 gatgaacatc acatcgacgg ttgacctgac gcaggcgtcg gaaatcctgc acccgcagac
     2581 caccctgcgc ggcgcaggaa acttcgctgg caaagtttcg ggtgaaggcg atcaatacaa
     2641 agttgacggc accatcaagt ccgacgcact cgcagcggat ggcgtgcgct tgcagggact
     2701 gaatgtcacg gcggcgggtt cggggcaggg gaagacttac aacatcaag
//
LOCUS       DASUEX010000442         2905 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1453495, whole genome shotgun sequence.
ACCESSION   DASUEX010000442 DASUEX010000000
VERSION     DASUEX010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2905
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1453495"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            33..2570
                     /locus_tag="VFV61_07505"
     CDS             33..2570
                     /locus_tag="VFV61_07505"
                     /inference="COORDINATES: protein motif:HMM:NF013239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIM-barrel domain-containing protein"
                     /protein_id="HEX4900363.1"
                     /translation="MQEMINQLVGYYRQLRSLRHGRIARRSWVFALLLVFAITVHAQD
                     ITQLEKIGPVVSFTKSEKAVVLNCADNSQVQVTLLAPDLVRIRASFTKPLPPRDHSWA
                     IAKTDWATPRWTLTEDQTSILITTDELEVVIRRSPLLIQFRDAKTHAVINADEQPMAY
                     DAKGLLKEMMFDSKAGTFVAAAKRLGFAEHFYGLGEKAARLDKRRSSFVNWNSDTPGY
                     LEGRDPIYQTIPFYTGLQNGQAYGIFFDNSYRSYFDFGKSSQLRIWFGAEGGELNYYF
                     FYGPSLKKILGRYADLTGRMPLPPLWALGNQQSRWSYYPDTMVQEVVAEYRKRDLPLD
                     VLHLDIDYMQGYRVFTWDRERFPNPKALTEKLARQGIKLVAIVDPGVKYQPVAKSAGQ
                     VTYTAPELEPQQQRYYVFDSGLEKNYFQRRQNGDLFVPKVWPGESVFVDYTLPAAREW
                     WGSLHRAYTDNGVAGIWNDMNEPADFIDQTGKNQLDVVSYDEGEKTTHAKNRNVFALL
                     MARATYEGLLRLQPERRPYVITRAAYAGIQRYSTMWTGDTNSTWEALQLNIPMFTTLG
                     MSGEPFVGSDVGGFIGRGNGELLVRAYQISFLAPFCRNHKVIDGYDNEPWRFGAYYED
                     IIRKYLKLRYQLLPFLYTALEEAHRTGVPLFRPLVLNYPEDANTHNLDDEFMIGDDLL
                     VAPVVKPDVTRRLVYLPKGTWYDYWTNKKYQGGTMISAEAPLETVPMFVRGGAIIPTG
                     PTMNYVGERPSDPITFNIYPDEKGSASATLYEDDGVSPAYKQDGFRRTTISVGATLGG
                     YTVSTGTPKGSYNPGPRKFSFLIKSATRMRSIVTVADNGAAQTVRIK"
BASE COUNT          681 a          788 c          782 g          654 t
ORIGIN      
        1 tcttatgtcg ggtcgaatac tggcgaacat agatgcaaga aatgattaat caactggtag
       61 gttactaccg gcaactcagg tcgctccgtc acggtagaat cgcgcgcaga tcatgggtct
      121 tcgctctcct tcttgttttc gccattaccg ttcacgcgca ggacatcacg cagcttgaaa
      181 agatcggccc ggtagtgagt tttacgaaat ccgaaaaggc cgtggtactt aattgcgccg
      241 acaactctca agttcaagtc acgctcctgg caccggatct ggtgcgcatc cgggcgagct
      301 tcactaagcc cttaccaccc agagatcact cctgggcgat tgcaaaaacg gattgggcca
      361 caccacgctg gacgcttact gaagatcaaa cttcgattct aatcaccact gacgaactcg
      421 aggttgtaat tcgtcgctcc ccactgctta ttcaatttcg cgatgctaag acgcatgcgg
      481 taatcaacgc tgatgagcag ccgatggcat acgacgccaa aggtctgttg aaagagatga
      541 tgtttgactc gaaagccggc acttttgtgg ctgccgccaa gcgcttaggg ttcgcggaac
      601 acttttacgg cctgggagaa aaagcggcgc gcctcgataa acgccgcagc tcatttgtta
      661 attggaattc agatacgccg ggttatctcg aagggagaga tccgatctat cagaccatcc
      721 ctttctacac cggtctgcaa aacggtcagg cctacggcat ttttttcgac aacagctatc
      781 gcagctactt tgatttcggc aagtcgtcgc aactacgaat ctggtttggc gctgagggtg
      841 gcgagcttaa ctattacttt ttttacggcc cttcactgaa gaagattctg ggacgctatg
      901 ccgatctcac cggtcgtatg ccattgccac ccctctgggc actcggcaac cagcagagcc
      961 gctggagcta ctaccctgac acgatggtcc aggaggtggt agcggagtat cgcaagcgag
     1021 atctgccgct cgacgtgctg caccttgata ttgattacat gcagggctac cgcgtattta
     1081 catgggaccg agaacgtttt cccaatccca aagcactcac tgagaagctg gcgcggcagg
     1141 gaatcaaact ggtggcaatc gtggatccgg gggtgaaata tcaaccagtt gcaaaaagcg
     1201 ccggtcaggt gacttacaca gctccggagc tcgaaccgca acagcagcgt tattacgtct
     1261 tcgattcagg tctggaaaaa aactactttc agcggcgtca gaacggtgac ctgttcgtgc
     1321 ctaaggtctg gcccggcgaa agcgtgtttg ttgattacac gcttcccgcc gcccgcgagt
     1381 ggtggggaag cttgcaccgc gcttatacgg acaacggtgt tgccggcatc tggaacgaca
     1441 tgaacgagcc ggcagacttc atcgatcaaa cgggtaagaa ccaactcgat gtcgtcagct
     1501 acgacgaagg tgaaaaaacg acgcatgcta agaaccggaa tgtgtttgcg ctcttaatgg
     1561 cccgggcaac ctacgaaggt ttgctgcggc tgcagcccga gcggcgaccc tacgtaatca
     1621 cgcgcgccgc ttacgcaggg attcagagat actcaacgat gtggactggt gataccaaca
     1681 gcacctggga ggcgctccag ttgaacattc ctatgttcac caccctcgga atgtcaggcg
     1741 aaccttttgt gggtagcgac gttggtggct tcatcggacg cggcaatggc gaactgttgg
     1801 tgcgcgccta tcaaataagt ttcctggctc ctttctgccg caatcacaag gtgatcgacg
     1861 gctacgataa tgagccctgg cgctttggag cttattacga ggacattatt cgcaaatacc
     1921 tgaagttgcg ttatcagttg ctgcctttcc tctacacagc attggaagaa gcgcatcgaa
     1981 cgggcgtgcc tttgttccga cctctcgtgc tgaactatcc cgaggacgct aatacgcaca
     2041 accttgatga tgaattcatg attggcgacg acctgctcgt agcacccgtg gtcaagcccg
     2101 atgtcacccg ccggctcgtc tacttgccaa agggtacctg gtacgactat tggacgaata
     2161 agaagtatca gggcggaacg atgatttctg ctgaggctcc tctggaaact gtgcctatgt
     2221 tcgttcgcgg tggagcgatc attccaaccg gaccgacaat gaactacgta ggcgagagac
     2281 cttcagatcc aatcacgttc aatatctatc cggatgagaa gggttcggcg tccgcaacgt
     2341 tgtacgagga cgatggagtt agcccggcct acaaacagga cggctttcgt cgcaccacaa
     2401 taagtgtcgg cgcaacgctc ggtggttaca ccgtgagcac tggtacaccg aagggtagct
     2461 acaatcctgg tccgcgcaag tttagtttcc taatcaagtc ggcgactcgg atgcgtagca
     2521 ttgtcacggt cgcagataac ggagcggcgc aaaccgttag aattaagtga gcctgattgt
     2581 ctcggctagc gttacgtttt cactccacag tcgtgatgct tggccccaaa aatcctgagc
     2641 tagcatcggt tcggctttgc cgccggattt gaggcgtggc ttgccgcgcc ggcagcggct
     2701 ggattgttcc ctgggcaatg ctcctcgttc gggcttgtcc ctttggattt actgggccaa
     2761 gaggccgcac taaggggcat agccccagta gccccagcta tcaaagtcat ctccgcggcg
     2821 cggcagagcc ggcaccgcac atccggcgac aaagccgaac ctgacatggc tcatgacttc
     2881 taaggcaaag ccgatccaaa cagaa
//
LOCUS       DASUEX010000443         2681 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_85396, whole genome shotgun sequence.
ACCESSION   DASUEX010000443 DASUEX010000000
VERSION     DASUEX010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85396"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1072
                     /locus_tag="VFV61_07510"
     CDS             <1..1072
                     /locus_tag="VFV61_07510"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX4900364.1"
                     /translation="QIQQRFDPDARPSPTPTPSPTPSGPKALTVQQLAEQTDQFLKDN
                     GAVVRINDAGREHGQIRAFNNRTFDLNKAVPTVVMRNEDYGRISRILADNTEVELEFE
                     IVNRVYPEGKTSYNTIAEIPGSDKADEVIMLGGHLDSWHAATGATDNAIGCAIMMEAA
                     RILKSIGVKPRRTIRVALWSGEEQGLLGSLAYVKEHFGSFENPKPGYEKFGGYFNIDS
                     GTGRIRGASVFGPPETGKIMRDLLKPFKDEGVAGAIATRNRRVGGTDSTSFNNAGLPG
                     IGLTQDTIEYGTHTWHTNLDTYERILEDDVKQDAVVVAFALYKLAMRDELLPRFSREA
                     MPPPAPVEPTPTPTPAPKPRRG"
     gene            1069..1695
                     /locus_tag="VFV61_07515"
     CDS             1069..1695
                     /locus_tag="VFV61_07515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900365.1"
                     /translation="MKVISGAVDKVLIQKLENTIIVLIGFAGTGKYTIGRELCELTGA
                     KLIDNHLINNPVFTVVNQDGIKPLPEGVWDKVKAVREIVYESIRELSPPQMSFVFTIE
                     LFESDPGDQRAFVDLEALAAARRSLFVPVRLICEVEELCRRIVDPARAQKLKLRNPET
                     ARRKAARESVLDSTHPHVLTIDVTSKTPNKTAEMILRHVAEIQALRTY"
     gene            complement(1715..>2681)
                     /gene="moaA"
                     /locus_tag="VFV61_07520"
     CDS             complement(1715..>2681)
                     /gene="moaA"
                     /locus_tag="VFV61_07520"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HEX4900366.1"
                     /translation="IRDLRVSLTDRCNFRCFYCLPHGEPPIAPKEQMLSYEEIEYVCE
                     IFVSLGIEKIRLTGGEPMLRRDIETIIAKLSVLKSKGLLDLALTTNGYYLPDRAQSLK
                     DAGLDRVTISVDSLKREVFQEMTGVDVLDRVLAGIAATRAAGLEPIKINAVIVRGHNE
                     GEVADFAAFAREQDVKMRFIEFMPLDSGHEWSRDMVVSGKEIRERISARFPLVTVDMP
                     RGSETSSRYRFADGAPGEIGIIAPVSEPFCGACSRIRLTADGQIRTCLFSTVEHSLRD
                     VVRSGATRAAIIEYIESVVLKKEPRHYINDPQFVAPSRTMSFIGG"
BASE COUNT          639 a          739 c          744 g          559 t
ORIGIN      
        1 acagatccag caacgttttg atcccgacgc gcggccctca ccgactccga cgccctcgcc
       61 gacaccgtct ggacccaaag cattaaccgt gcagcaactc gcggaacaga cagatcaatt
      121 cctaaaggac aacggggctg tggtccgcat aaatgatgcc ggccgcgaac atggccagat
      181 ccgtgccttc aacaatcgca ctttcgatct gaacaaagcg gtgccgacgg tggtgatgcg
      241 caatgaggat tacgggcgga tctcgagaat ccttgcggat aacaccgaag tggaattgga
      301 gtttgagatt gtcaaccgcg tctatcctga aggtaagact tcttacaaca ccattgccga
      361 gatcccgggt tcagacaaag ctgacgaagt gatcatgctg ggcggtcatt tggattcctg
      421 gcacgccgcc actggcgcaa ccgacaatgc gatcggctgc gcgatcatga tggaggcggc
      481 acgcatcctc aagagtattg gcgtgaaacc gcggcgcacg atccgcgtgg cgctttggag
      541 cggtgaagaa cagggactgc tcggctcgtt ggcttatgtg aaagaacact ttggctcgtt
      601 cgaaaatccc aaaccgggtt acgagaagtt cggtggctat ttcaatatcg actccggcac
      661 tggccgtatt cgcggcgcct cggtttttgg tccgccagaa accggcaaga tcatgcgcga
      721 cctgctcaaa cccttcaaag atgaaggcgt cgccggcgcg attgctacgc gcaaccgaag
      781 agttggcggt actgacagca cttctttcaa caacgccgga ctgcctggga tcggactgac
      841 gcaggatacg attgagtatg gcacgcacac ctggcacacc aatctcgata cctatgaacg
      901 cattctggaa gacgatgtga agcaggatgc ggtagtcgtc gcgttcgcgc tttacaagct
      961 ggcaatgcgc gacgagttgt tgccacgctt ttcccgagaa gcgatgccgc cgcctgcacc
     1021 cgtcgaacca acgccgacac caacgccagc ccctaaacca aggcgtggat gaaggtgata
     1081 agcggagcgg tggacaaggt gctcattcaa aaacttgaga acacgatcat tgttttgatc
     1141 ggctttgccg gaaccggcaa gtacacgatc ggtcgcgagc tttgcgaact caccggcgca
     1201 aagctgatcg acaatcactt aatcaacaac ccggtattca ccgttgttaa ccaagatggt
     1261 atcaaaccgc tgcctgaagg tgtctgggac aaagtgaaag ctgtccgcga aatcgtgtat
     1321 gagtcgatcc gcgagctatc gccaccgcag atgagttttg tgttcactat agagctcttc
     1381 gagagcgatc ccggcgatca aagggccttc gtcgatctgg aagcgctagc ggcagccaga
     1441 cgaagtttgt ttgttcccgt gcgattgatt tgtgaggtcg aagagttatg ccgccgcata
     1501 gtcgatccgg cgcgcgctca aaagctcaag ttgcgtaatc cggaaacggc acggcgcaag
     1561 gcggccaggg agtcagttct agattccacg catcctcatg tgttgactat cgacgtgacg
     1621 agtaagacac ccaacaagac cgcggaaatg attttgcggc atgtggcgga gattcaagca
     1681 ttacggactt attaactgtc ctgtccggac tgtcctatcc accaataaaa ctcatcgtgc
     1741 gtgagggagc gacaaactgc ggatcgttga tgtagtgacg aggttctttt ttcaaaacga
     1801 ctgactcgat gtattcgatg attgccgcgc gcgttgcacc gcttcgaact acatcgcgca
     1861 gactgtgttc gactgtcgag aataaacagg tgcgaatctg cccgtcggcg gtgaggcgaa
     1921 tgcgagagca ggcaccgcag aagggttcgc taacaggcgc gatgattcca atctcgccgg
     1981 gtgcgccatc agcaaaccga taacgcgacg acgtttccga accgcgcggc atatctaccg
     2041 tcacaagcgg gaagcgcgcg ctgattcgct cgcggatttc cttcccggaa accaccatat
     2101 cacgcgacca ctcatgtccg gaatcaagcg gcatgaattc gatgaagcgc atcttcacat
     2161 cttgctcgcg ggcaaaagct gcaaagtcag caacctcgcc ttcgttatga ccacgcacaa
     2221 tcacggcgtt gatcttgatc ggttccagtc cggcagctct ggtagccgcg atgcccgcca
     2281 ggacgcgatc cagaacatca acacccgtca tctcctggaa cacttcgcgt ttcaaactgt
     2341 cgacactgat ggtcacgcga tcgagtccgg cgtccttgag gctctgggcg cggtccggaa
     2401 gatagtaacc attggtggtg agtgcgagat cgagcagtcc ttttgatttg agcactgaga
     2461 gcttcgcaat aatggtttca atgtcgcgtc gcagcatcgg ctcgccacca gtaagtcgga
     2521 tcttttcgat gcccaggctg acaaagatct cgcagacgta ttcgatctct tcgtatgaga
     2581 gcatctgctc tttgggcgct atcggcggtt cgccgtgcgg caggcaatag aagcagcgaa
     2641 aattacagcg atcagtcagc gacacgcgca ggtcgcgaat c
//
LOCUS       DASUEX010000444         3422 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_644474, whole genome shotgun sequence.
ACCESSION   DASUEX010000444 DASUEX010000000
VERSION     DASUEX010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3422
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_644474"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..832
                     /locus_tag="VFV61_07525"
     CDS             <1..832
                     /locus_tag="VFV61_07525"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX4900367.1"
                     /translation="PLALKLLAPYRDFRRETYGALTYYSFNLSHEPFDDVRVREALAI
                     AIDRDRLAQDEMGGATEPAKKFLPELKKESAKVVVSKTESLELDAERARELLAEAGYP
                     EGHGFPVVRLLINRNEQQRLIANSIAAMWRSILNIETEISIKGWDEYEAAITAGDYDV
                     VRRGMVMQTTDELTNIRALFQQDSRPVALPTNSQAATNGSGGVSKEKAEPAKAPPLES
                     EADALKELRAIPLYFASSHALIKPYVTGFDSNLLDAPSLRRVRMETNWTAPKNANSGR
                     "
     gene            1038..3020
                     /locus_tag="VFV61_07530"
     CDS             1038..3020
                     /locus_tag="VFV61_07530"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX4900368.1"
                     /translation="MNIDCIGKRIIIAILLAASALVSLSCNVAAGNEEFFGKTTPPEG
                     NILRSVNGPEPESFDPAISSGQPEARIYMALFEGLVEYHPKSLDAIPAIAERWDTNND
                     SSEFVFHLRHNARWSNGDPINAHDFIYSLRRALSPEVQSRTANLAYYIKYAQAYNSKS
                     SFVRDPQTGKFLLEQDFATEPATTPLSLSQKALGAGESEYTLGDGKSAAPDTQFHQYM
                     HSPARLVLPGSEKARQKMLDANPKLKAAVANAELVVVTGEDVGIEAVDDYTVRISLAQ
                     PAPYFTGLAAHQLFRLVPRKIIETYGDRWTDPANIVTCGPFKLKSWKPYHEVVVERDP
                     MYWDAANVHLDEIRFYVSLDHPTSMNLYKVGAADAVVNHSVPHAWVDVVRPKKDFMDA
                     AEAAITYLLINVTQPPMNDLRVRRAFNMAIDKETYSKSKRTTKPLSAFTPTGIFVGYP
                     QPQGEAFNPAEARRLLGEAGYPVTQKPDGTYVCEKFPTDEVKLMFATHSSNKTLCEFM
                     QAQWKQNLGITVMLNNLEAKTYMAARGKLEYKGFALGIWGADYMDPSTFLNLFLTPGG
                     DNGSGWWDKHYVDLLETANHTADKQKRYELLAKAEKYMLDAQPTIPLETPSVNWVKKP
                     YVKGLYPNPSSLFAWKFVYIERDPAKWDYRTPSLAE"
     gene            3050..>3422
                     /locus_tag="VFV61_07535"
     CDS             3050..>3422
                     /locus_tag="VFV61_07535"
                     /inference="COORDINATES: protein motif:HMM:NF040248.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900369.1"
                     /translation="MLRFIIKRLLITIPTILVVITITWGLVRLAPGNFYTGEKKLPPA
                     IERNIREKYGLDKPWYEQYRRTMWGIVRHLDFGTSLTYPGQSVNAIIARSLPISATVG
                     LLAYLFAVVIGVTIGASAAVKP"
BASE COUNT          806 a          946 c          917 g          753 t
ORIGIN      
        1 gcctttggct ctgaaactgc tcgcccctta ccgggacttc cggcgcgaaa cctacggagc
       61 cctgacctac tacagtttca acttatccca cgagccgttt gacgacgtgc gcgtgcgcga
      121 ggcgctggcg attgcgatcg atcgcgacag gctggcgcag gatgagatgg gtggcgccac
      181 tgagccggcg aaaaaattcc ttcccgagct aaaaaaagaa tccgcgaagg tcgtagtttc
      241 aaagaccgaa tcgctcgaac tagacgcgga gcgcgcgcgc gagttgttag cagaggctgg
      301 ctatccggag gggcacgggt ttcccgttgt gcgcttgctc ataaatcgga acgaacaaca
      361 acggctgatc gcgaactcga tcgcggcaat gtggcggagt attctgaaca tagaaactga
      421 gattagcatc aagggttggg acgagtacga agcggcaatt acggctggag actatgatgt
      481 ggtgcggcgc gggatggtga tgcaaaccac agacgagttg acgaatattc gcgcgctctt
      541 ccaacaggac agccggcccg ttgctttacc taccaattcc caggcggcaa caaacggatc
      601 gggaggcgtc tccaaagaaa aagcagaacc cgcgaaggcg ccaccgctgg agtcggaagc
      661 ggatgcgtta aaagagctgc gggcgatacc actctacttt gcctcgtctc atgcgcttat
      721 caaaccctat gtgactggtt ttgattccaa cctgctggat gctccctctc tcaggcgtgt
      781 gcgcatggaa acgaactgga cggcgccgaa gaacgccaac agcggaagat agaagaacat
      841 tgccaacttc caattgccag tttccgattg tcgcctcgcg ccacgagcca attggcaatg
      901 ggcaatcgcc aattggcatt cggggtagtg gggtaatagc gtgttggtcc tactaccgca
      961 tttcagctgt ttcttgatca aagtatttcc gcgtgttaac ttttcggctc gtgtcgcact
     1021 cttgcgaagg tgatcacgtg aatatcgact gcatcggtaa gcgaatcatc attgcgattc
     1081 ttctggcggc ttctgccctc gtatcactta gctgcaatgt ggccgccggc aatgaagagt
     1141 ttttcggcaa gaccactcca ccggaaggta acatcctccg ctctgtaaac ggtcccgagc
     1201 cggagtcgtt tgacccggca atcagttctg gtcaaccaga agcgcgcatc tacatggcgc
     1261 tcttcgaagg cttagtcgaa taccatccca aatcgctcga cgcaattccc gccattgccg
     1321 aacgctggga cacgaataac gattcatcgg agtttgtctt ccatttgcgt cacaacgcgc
     1381 gctggtcgaa tggcgaccct attaacgcgc acgacttcat ctacagcctg cgccgcgctt
     1441 tgtcgcctga agttcaatcg cgaaccgcga acctcgctta ctacatcaag tacgcccagg
     1501 catacaattc caagtcctcc tttgtgcgcg atccgcaaac ggggaagttc ttgctggagc
     1561 aagacttcgc cacagagcca gccaccacgc cgctttcctt gagccaaaag gctttaggcg
     1621 ccggtgaatc ggaatacaca cttggggacg gaaagtctgc tgcccctgat acccagtttc
     1681 atcagtacat gcattcaccg gcgcgtctgg tacttccggg aagtgaaaag gcgcgtcaga
     1741 aaatgcttga cgctaatccc aagctgaaag cggctgtggc aaacgcggaa ctcgtggtgg
     1801 taacgggcga ggacgtcggc atcgaagccg ttgacgatta caccgtgcgc atctcgctcg
     1861 cgcagccggc gccatatttc accgggctag ccgcacatca gctttttcga ctcgtgccac
     1921 gcaaaatcat cgagacgtac ggcgaccggt ggactgatcc ggcaaatatc gttacctgcg
     1981 gcccattcaa gttgaaatcc tggaaaccct accacgaagt ggtggtggaa agagatccga
     2041 tgtactggga cgcggcgaat gtgcatttgg atgaaatccg tttctacgtt tcgctggatc
     2101 atccgacgtc gatgaatctt tacaaggtcg gagcggctga cgctgtggtt aaccactcgg
     2161 tgcctcatgc ctgggttgat gtggtgcggc ccaagaagga ctttatggac gctgcagaag
     2221 cggccatcac ttacctcctg atcaatgtca cacaaccgcc gatgaatgat ttgcgtgtcc
     2281 gccgcgcttt caacatggca atcgacaagg agacctactc gaagtcaaag cggaccacta
     2341 aacctctttc ggcatttacg cccacgggaa tctttgttgg ttatccgcaa ccgcagggag
     2401 aagccttcaa tccagccgaa gcgcgcagac tattaggcga agctggctat cccgtcacgc
     2461 agaaaccgga cggcacctat gtctgcgaga agtttcctac cgatgaagtg aagttgatgt
     2521 tcgcaaccca ttcttctaac aaaacgcttt gtgagttcat gcaggcgcaa tggaagcaga
     2581 atttgggaat cacggtaatg ctcaacaacc ttgaagcaaa aacctacatg gccgctcgcg
     2641 ggaagctgga gtacaaggga ttcgccttag gaatttgggg cgccgattac atggatccgt
     2701 caaccttcct aaatctgttt ctcactcccg gtggcgataa cggcagcggc tggtgggaca
     2761 agcattacgt tgacttgctg gaaacggcga atcacactgc cgacaaacag aagcgctacg
     2821 agctgctggc taaagcagag aagtacatgc tcgacgctca accaacgatt cccctcgaga
     2881 cgccttcggt gaattgggtg aagaaacctt acgtcaaggg tctctatcca aatccatcct
     2941 ccttgtttgc ctggaagttt gtgtacatcg aacgcgatcc ggcgaagtgg gattatcgca
     3001 ctcctagttt ggctgagtag cagacaggca ccctgtgcct gtctttctta tgcttcgttt
     3061 catcatcaaa cgtttgctga tcaccattcc cacgatcctg gtggtaatta ccattacctg
     3121 ggggttggtc agactggcgc cgggaaactt ctataccggc gagaagaaac tgccgccggc
     3181 gattgaacgg aacattcgcg aaaagtacgg actcgacaaa ccatggtatg agcaataccg
     3241 gcgaacaatg tggggtatcg tgcgccacct cgattttggt acgtcgctca cataccccgg
     3301 ccaatcagtt aatgccatca tcgcccgctc gcttccgatt tctgccactg tcggcttgct
     3361 ggcttacttg ttcgctgtcg ttatcggcgt gacgatcggc gccagcgccg cagtcaagcc
     3421 aa
//
LOCUS       DASUEX010000445         2758 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_886348, whole genome shotgun sequence.
ACCESSION   DASUEX010000445 DASUEX010000000
VERSION     DASUEX010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2758
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_886348"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(151..>2758)
                     /locus_tag="VFV61_07540"
     CDS             complement(151..>2758)
                     /locus_tag="VFV61_07540"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HEX4900370.1"
                     /translation="QPLGLNAKRVISKRYSLKDAKGRALEEWPDIVRRVVGHVSAAEK
                     DSARRDEFFAAMSEVMLAREFVPNTPCLVNAGKANGQLAACFVLDVPDSITGIMEHAK
                     AAATIHQTGGGTGMTYEFLRPHGALVGSTRGVASGPVSFMNIVNQVTDVVKQGGVRRG
                     ANMGMMRVTHPDVLRFIHAKNDQHSLTNFNISVNVTDKFLKAVDDNEWFQLEFDGESW
                     TDPIYDPVRDGDYLVYRRPDGSTVTFPDKQAFIAADLSNCTIEEPPRPGMIYAPDIWN
                     RIVASAHKYAEPGIAFIDEVNRHNHMMKSMGPIYSCNPCGEQFLHFSNSCNLGSIDLA
                     KFYDPETRIDWDRLREVTHLCTRFLDNVIDTCAWPLPEINDVVSRTRPVGLGIMGFAD
                     LCLDLKVTYGSPASIDLMEEMMGFIRRESWNASLQIGAEKGTFPEFEPNREAYEEFLY
                     NQVGISRDVPLTPRNYEVTTIAPTGTISLVAETSSGIEPNFSWAYVRKDTLGTRTYVH
                     TLAAQALGLEVDQTDADSIDAAATYVCEHENELPQYFISAMNISAEQHVHVLAAAQRN
                     VDNSVSKTCNGAINDTVESVDRLYRLGRQLGCKAVSYYRDGSRDNQVLNSMKASDSET
                     KSETACNPEAGAEVLDDPPASHSDAAHVIPMDPETEGLARAEAGQMIRAQADAEATLT
                     AIRIERPRELRGATWQIPFDGQNLYVTVNHDSQMIQEVFATGPISGGVGLLASKMLRG
                     GFDAAEVAYSLNKVTGTHAVWFNERLLTSPEQAVAECIMITNRRLTDLPDSARALAKQ
                     QHSGAQIPLSGSSNAGANASQATGSTSAGLTGVTGAIMSTMIGICPECKGQLEHASGC
                     DFCRDCGYSKCK"
BASE COUNT          615 a          804 c          745 g          594 t
ORIGIN      
        1 accggaagac gatccccgaa gctcacgaaa cgacccgaaa accgccagtg ctttggacta
       61 tacctgagtc gcgccgcgaa gacccttaag gtaaaagccg cgacagtgca tcgcggcttt
      121 caacctatga ggcgagctca actggctcca tcacttacac tttgaatacc cacaatcccg
      181 acaaaaatca cagccgctcg cgtgctcgag ttggcctttg cactcggggc agatgccgat
      241 catcgtggac atgattgcgc cggtgacgcc agtcaaacct gccgacgtgc tgccggttgc
      301 ctgcgaagcg ttggcgccgg cgttcgaaga tccacttaaa ggaatctgcg cgcccgaatg
      361 ttgctgtttg gcgagcgcgc gtgcggaatc cggcagatca gtcaaacgcc ggttcgtgat
      421 catgatgcac tcggcaactg cctgctccgg tgaagtcagc aaacgctcgt tgaaccaaac
      481 cgcgtgtgtg cccgtcactt tgttcaaact gtaagccacc tccgcggcgt cgaagccacc
      541 acgcagcatc ttcgacgcca gcagacccac gcccccgcta atgggaccgg tagcaaaaac
      601 ttcctgaatc atctggctat cgtgattgac ggtgacgtag agattctgcc cgtcaaaagg
      661 aatctgccag gttgcgcccc gcaactcgcg cgggcgctcg atgcggattg ctgtcaatgt
      721 cgcttcggca tccgcttggg ctctgatcat ctgaccggct tcggcgcgag ccagtccttc
      781 cgtttccgga tccattggta tcacgtgcgc tgcatccgaa tgactcgccg gcggatcatc
      841 cagcacttcc gcgccagctt ccggattgca cgcagtttca ctcttagtct cggagtctga
      901 cgccttcatc gagttcagca cctgattgtc gcgcgaaccg tcgcgatagt agctgacggc
      961 tttgcagcca agctggcggc ctaagcggta gagccgatct accgactcga ctgtgtcgtt
     1021 gatggcgccg ttgcaagtct tcgaaacact attgtccacg ttgcgctgcg cagcggcgag
     1081 cacgtgcacg tgttgttcag cggagatatt catcgcggaa ataaagtact gcggcagttc
     1141 gttctcgtgc tcacaaacat aagtcgccgc agcatcaata gagtccgcgt ccgtctggtc
     1201 aacttccaaa cccaaagcct gcgctgcgag cgtatgcacg taagtccgag tgccgagcgt
     1261 gtctttgcgg acgtaagccc aggagaagtt tggctcaata cccgaagaag tttcggctac
     1321 cagcgaaatt gttcccgtcg gcgcgatcgt cgttacttca tagttacgag gcgtcaacgg
     1381 aacgtcgcgc gaaataccaa cctggttata gagaaactct tcataagctt cgcgattcgg
     1441 ttcaaactcc gggaacgtcc ctttctcggc gccgatctgc agactcgcat tccaggattc
     1501 gcgtcggatg aagcccatca tctcttccat caaatcgatg gaagccggcg aaccataagt
     1561 caccttgaga tccagacaga ggtcggcgaa acccataatc cccagtccga ctgggcgcgt
     1621 ccggcttacg acatcgttga tttccggaag tggccaggcg caagtatcaa ttacgttgtc
     1681 gagaaaacgg gtgcataggt gggtcacttc gcgtagtcga tcccaatcga ttcgggtttc
     1741 gggatcatag aacttcgcga gatcaatcga gcccaggttg cacgagtttg aaaagtgcag
     1801 gaactgctca ccgcagggat tgcaggaata gattggcccc atcgacttca tcatgtggtt
     1861 gtgacggttt acttcgtcga tgaaggcgat tcccggttcg gcgtacttgt gcgcactggc
     1921 aacaatccgg ttccagatgt cgggtgcgta gatcatgccg gggcgcggcg gctcttcgat
     1981 cgtgcaattc gaaagatcgg cggcaatgaa cgcctgcttg tcagggaatg tgacagtgga
     2041 accgtcggga cggcggtaga caaggtaatc gccgtcgcga accggatcat aaatcggatc
     2101 cgtccacgac tcgccgtcaa actcgagctg gaaccattcg ttatcgtcga ccgctttgag
     2161 aaacttgtca gtcacgttta ccgagatatt gaagttggtc agcgagtgct gatcattctt
     2221 ggcatgaatg aagcgcaaga cgtcaggatg agtaacacgc atcatcccca tgttggctcc
     2281 ccgacgaacc ccaccctgct tcaccacatc cgtgacttga ttgacgatgt tcatgaaaga
     2341 aacaggaccc gaggctacgc cgcgcgtcga acctaccagc gcaccgtgcg gtcgcaggaa
     2401 ctcataggtc attccggtgc cgccgccagt ctggtgaatt gtggcagccg ccttagcgtg
     2461 ttccatgatc cccgtgatcg aatccggaac atcgagaaca aaacacgcag cgagttgacc
     2521 attggctttg ccggcgttaa cgaggcaagg agtgttgggc acaaactcgc gcgccagcat
     2581 cacctcgctc atggccgcaa aaaattcatc ccgcctggct gaatcctttt cggccgcaga
     2641 cacatgaccc acaacacgac gaacgatatc cggccactct tccaaggcgc gtcctttcgc
     2701 gtctttcaac gagtagcgct tactgattac acgtttggca tttagaccca ggggttga
//
LOCUS       DASUEX010000446         2905 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1575231, whole genome shotgun sequence.
ACCESSION   DASUEX010000446 DASUEX010000000
VERSION     DASUEX010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2905
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1575231"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1218)
                     /locus_tag="VFV61_07545"
     CDS             complement(<1..1218)
                     /locus_tag="VFV61_07545"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX4900371.1"
                     /translation="MATLSFWGGVGTVTGSKYLIETDKARVLVDCGMFQGLKELRERN
                     WQEPPFDPRALDAVLITHAHIDHTGYLPRMVRQGFNGPVYCSRGTADLLKILLPDAGR
                     LQEEDADYRNRHKITRHTPALPLYTEKDAFATLEQVRPLPNDGVPVEVAQGIRAGFRI
                     AGHILGSSLVLVEIAGAGADGAGRRVLFSGDLGHYDQPIIHDPVAPPLCDYLLVESTY
                     GDRLHDPENPKAALTRIITDAANRNGVVLIPAFAVGRTQELIYLIRELEDEKAIPVLP
                     VYVDSPMAAASTAAYANRKEEQDEDYASVLARQKHPLRTHSMTTATSKAQSKSLNEAQ
                     GARVIISASGMMTGGRVLHHAIRLLPDPNTTLVFVGYQAAGTLGRRILDGEQDVRILG
                     QQIPVRCRLDKIGG"
     gene            complement(1235..1795)
                     /locus_tag="VFV61_07550"
     CDS             complement(1235..1795)
                     /locus_tag="VFV61_07550"
                     /inference="COORDINATES: protein motif:HMM:NF018942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HEX4900372.1"
                     /translation="MHAQTSGDFTVSPRVRISVSVLKWLVPISRFGLAALFLLTAGAK
                     LAIVKDFAGNVAQLVSASGFDYARWTWPATIAVIVAELITAALLLVPKTVRVGALCAA
                     ALLLGFAGFALYYVYVLHGEPLECGCFGGIIGSQLGVKTALRNLGLLIPALLVFFGYR
                     RARTQRAVYPPAQASDSLRGPAATDN"
     gene            complement(1864..>2905)
                     /gene="sucC"
                     /locus_tag="VFV61_07555"
     CDS             complement(1864..>2905)
                     /gene="sucC"
                     /locus_tag="VFV61_07555"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF001913.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEX4900373.1"
                     /translation="EIVVVKAQIHAGGRGKGGGVKLAKSADEAREIARQMLGMKLVTH
                     QTGPEGREVRVLLIEEGLPIDKEFYLGVVLDRASGRPVFMASAAGGMDIEEVAAKTPE
                     KILKETIDPAVGFRSFQARKLAFALGLPAPLINQAVKFMQSLYNAYEQMDASLLEINP
                     FLLTKDSRLIALDAKVNFDDNALFRHKEFLDLRDLNEEDPLEIEASKFDLNYIKLDGT
                     IACMVNGAGLAMATMDIIKLAGGEPANFLDVGGGASQERVEAAFRILLADPNVKAVLI
                     NIFGGIVRCDMVARGVVEAVKNLGIKVPVVVRLEGTNVEEGQRVIRESGMNFTVANGM
                     KDAADKVVALAA"
BASE COUNT          645 a          858 c          789 g          613 t
ORIGIN      
        1 gccgccgatc ttatccaaac ggcagcgcac gggtatttgc tgaccaagaa tgcgcacgtc
       61 ctgttcgcca tcgagtatgc ggcggccgag agttcccgct gcctgatagc caacaaacac
      121 aagtgtggtg tttggatccg gaagcaaacg aatcgcgtga tgcaagaccc tgccaccagt
      181 catcattcct gacgcactga tgatcactcg cgcaccttga gcctcgttca aactcttcga
      241 ctgcgctttt gaagtcgcag tagtcatcga atgggtccgc agcggatgct tttgccgcgc
      301 caagacgctg gcgtagtctt cgtcctgttc ttccttgcga tttgcataag cggctgttga
      361 ggccgcggcc atcggtgaat cgacgtagac aggcaagacc gggatcgcct tctcgtcctc
      421 aagctccctg atcagataaa tcaattcctg tgttcttccc actgcaaatg cgggaatgag
      481 aacgacgccg ttacggttcg cagcgtcagt gatgatgcga gtcagagctg ccttggggtt
      541 ctccggatca tgcaggcggt cgccataggt cgattccacg agcaggtaat cgcagagtgg
      601 cggcgcgacg gggtcatgaa tgatgggctg gtcgtaatgt cccaggtcgc cggagaacaa
      661 aacgcgacga ccagcgccgt ctgcgcccgc gcctgcgatc tccacaagca ctagactgga
      721 accgagaatg tgacctgcga ttcggaagcc tgctcgaatt ccctgcgcca cttccacagg
      781 tacaccgtcg ttcggcagtg gtcgtacttg ctccagggtt gcgaaggcgt ccttctccgt
      841 gtagagcggc agcgccggcg tatgacgagt gatcttatgc cggttgcgat agtccgcgtc
      901 ttcttcctgc aagcgcccgg cgtcaggtaa gagaatcttg agcaagtcgg ctgtgccacg
      961 cgaacagtaa accggtccgt tgaatccctg tctgaccatg cgcggtaaat agccggtatg
     1021 atcgatatgg gcgtgagtaa ttagaacagc atcgagcgcg cgtggatcaa aaggtggttc
     1081 ctgccagttg cgctcgcgca gttccttcaa gccctggaac atgccgcagt ctacgagcac
     1141 gcgtgccttg tccgtttcga tcagatactt cgatccggtg acagtcccaa cgccgcccca
     1201 aaatgaaagt gttgccatca gtgtcccgag taatctagtt gtccgttgcc gctggacccc
     1261 tcagcgagtc tgacgcttga gccggaggat agaccgcgcg ctgagtgcga gcgcggcggt
     1321 aaccgaagaa gacgagtaac gccggaatca gaagtcctaa gttgcgcaat gcagtcttaa
     1381 ctccaagttg gctgccgatg atgccgccaa agcagccgca ctcaagtggc tcgccgtgga
     1441 gaacatagac gtagtaaagc gcgaagccgg caaatccaag cagcagcgcc gccgcgcaca
     1501 acgcaccgac gcgaaccgtt ttgggcacta acagcaaagc tgcggtgatt aactccgcga
     1561 cgatgactgc gattgtcgcg ggccacgtcc agcgcgcgta atcaaaaccc gaagccgaaa
     1621 ccaattgagc aacgttgccg gcaaaatcct tgacgattgc taacttggcg ccggcggtga
     1681 ggagaaaaag cgcggccaag ccaaaacggc tgatgggcac gagccatttc agtactgaca
     1741 cggagatgcg gacacgcggc gatacggtga aatcaccaga agtctgtgcg tgcatgggtg
     1801 caccaacacc ttcactgttc ataattatcg cgtctccgtc tctccgtgcc tccgggtctg
     1861 ctttcatgca gccagggcta ccactttgtc agccgcgtct ttcatgccgt tcgctacggt
     1921 gaagttcatc cccgactcac gaatcacgcg ttggccctct tcgacattag ttccttccaa
     1981 ccgcaccacg acaggaactt tgatccccag attcttcaca gcttcgacta cgccgcgcgc
     2041 taccatatcg caacgcacaa ttccgccgaa gatattaatc aacaccgcct tcacgtttgg
     2101 atccgctaag agaatgcgaa acgccgcctc gactcgttct tgcgaggcgc caccgccgac
     2161 gtccagaaag tttgcgggct cgcctccggc caacttgata atgtccatcg tcgccatggc
     2221 caggccagct ccgttcacca tacaggcaat ggtgccgtcg agcttgatgt aattgagatc
     2281 aaatttcgag gcttcaattt ccagaggatc ttcttcgttc aagtcgcgca aatcgagaaa
     2341 ctctttgtgg cgaaagagag cgttgtcatc aaagttgacc ttggcatcaa gggcgatcaa
     2401 gcggctgtcc ttggtgagca gaaaaggatt gatctcaaga agtgaggcgt ccatttgctc
     2461 gtaggcgttg tagagcgact gcataaactt aacagcctgg ttgattaacg gcgccggcag
     2521 gccgagggca aaagccaatt ttcgcgcctg aaatgagcga aagcccactg ccggatcaat
     2581 ggtctccttc aagatctttt ctggagtctt ggcggccacc tcctcgatgt ccatgccccc
     2641 ggccgcggac gccatgaaca ccggccgtcc cgaagcgcga tccaagacga cacccaggta
     2701 gaactcctta tcgattggca acccttcttc aataagcagg acgcgaactt cgcgaccctc
     2761 aggcccagtc tgatgcgtga cgagcttcat ccctaacatc tggcgcgcga tctcgcgtgc
     2821 ttcatcagcg cttttcgcta gtttgacgcc accacccttg ccacggccgc cggcatggat
     2881 ttgcgcttta actacgacga tctcg
//
LOCUS       DASUEX010000447         2209 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_85666, whole genome shotgun sequence.
ACCESSION   DASUEX010000447 DASUEX010000000
VERSION     DASUEX010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85666"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2097
                     /locus_tag="VFV61_07560"
     CDS             <1..2097
                     /locus_tag="VFV61_07560"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HEX4900374.1"
                     /translation="YSGDLKSFIRSLAEQLSLNVVFDRQSFAQSRTIDLTLRDVTTAQ
                     ALDYVFLQESLFFQKLSRRTILVADQTRRPQYQQLVLRTFFLSNIDPTEARTLVTQAI
                     PPSVGRPQTIVVPDKATNSLTVRDTAENVRLIGELLQSIDKDRAEVVMDVNIYEVSKN
                     DLLQFGNQFGTGNFNLGGTPGLAILSSSGVTTAAQTGVNLASIVAGAPTAAAAALVIP
                     PSVLTAIQSKDNTRLIASTQVHAFNGEESTARIGQRVPVQTAQAYPFGIQTGQPSTPG
                     GLPTGGFPVINYEPTGLTLKFTPQVFPNLDVQVKMSIESKDVLGAGTLTPTFTERTIT
                     GTARVQNNRTMMLASVAQDVQTNGRRGFPVLGLIPVLGRLFTSPTRDNRQIDIVIAVT
                     PKVLRAPNVTPRDEEMRPSGTLQSPTTGSLEAMIRDSDREDQLAAARRLPRNAVVEVK
                     NVEPPPFSPGNSRGDQTAVQNNGSNSQPVASATNSPQANPVIPPAVSNAKSQAPAEEL
                     PAFVPAPKSLVSDRAANEIAAINPGPSGNGNAVLTSLPQPIETSLNTKRARTATLSLP
                     PDEVLSVGQKRQFAVELNSGVPLGLAMLALRFNPKVVKVHAVSPGGLFAEVKDKGPSI
                     TQSIDPSGVCLISISTLNGGAPMSGTGALLLIEIEGLTPGDAALAFDSETMHLVAIDA
                     RDVVLEVKQGRMTVKQ"
BASE COUNT          520 a          601 c          598 g          490 t
ORIGIN      
        1 tacagcgggg atctaaaatc tttcatccgc agcctggctg aacaattaag cctgaacgtt
       61 gttttcgacc ggcagtcctt tgcgcaatct cgcaccatcg atttgactct gcgtgatgtc
      121 accacagcgc aagccctcga ttatgtcttt cttcaggaaa gtctcttctt tcagaaatta
      181 agccggcgca caattctggt agccgatcaa accaggcgtc ctcagtatca gcagttagtg
      241 ctgcgaacat tctttctttc gaacattgat ccaacggaag cgcggacgct cgtgacgcaa
      301 gcgatccccc cgtcagtagg acggccgcag acaatcgtgg tgcccgataa ggcgacgaac
      361 agtttgacag tgcgtgatac cgccgagaac gttcgcttaa ttggtgaact gctgcagagc
      421 atcgataagg atcgcgccga agtggttatg gatgtgaaca tctatgaagt ctcgaaaaac
      481 gatctgctcc aattcggaaa ccagtttggg actgggaact tcaaccttgg tggtactccc
      541 ggcctggcaa ttttgagctc ttcaggtgtg accacagccg cgcagaccgg tgtaaatctg
      601 gccagtattg tcgcaggcgc tcccaccgcc gccgccgctg cattggtgat accgccctca
      661 gtgctgactg ctattcaaag caaagacaac actcgattga ttgcctcgac ccaggtccac
      721 gctttcaacg gcgaagaatc cactgcacgt attgggcaac gtgtgccggt gcaaaccgca
      781 caggcttatc cttttggcat ccaaaccggc caaccgagta ctccaggtgg tctgccgacc
      841 ggcggctttc cagttatcaa ctatgaaccg accggtttaa ctctgaaatt tacgccgcag
      901 gttttcccca acctcgacgt gcaagtaaaa atgtccattg agtccaaaga cgtgctcgga
      961 gcgggaacac tgacccccac gttcacagaa cggaccatca ccgggaccgc tcgggtgcag
     1021 aacaatcgca cgatgatgct ggcaagcgtg gcacaggatg tacagaccaa cgggcgacgc
     1081 ggatttcccg tgctgggttt gattcccgtg ctgggtcgct tgttcacttc gcccacgcga
     1141 gacaatcggc aaatagatat tgttattgcg gtaacgccca aagtgttgcg agcgcctaat
     1201 gtgactcctc gggacgaaga gatgcggccg agtggaacat tgcagtcacc gactaccggt
     1261 tcgctcgaag caatgattcg tgactccgat cgcgaggacc agctagccgc cgcaagacgc
     1321 ttaccgagaa atgcggtggt ggaagttaag aacgttgagc cacctccatt ttctcctggg
     1381 aacagcaggg gcgatcaaac agccgtgcaa aataacggtt cgaattccca gccggtagca
     1441 agcgcgacaa acagccctca ggctaatcca gtgatcccac ctgcagtgtc aaatgccaag
     1501 agccaggcgc cagcagagga gttgccggcg ttcgttcctg cgccaaagtc gttggttagt
     1561 gatcgtgcag ccaacgagat agcggcaatc aatcccggtc cgtccgggaa tggcaatgca
     1621 gttcttacca gcctgccgca gccaattgag acttctctca acacgaagcg ggctcgcacc
     1681 gcgacactca gtttgccgcc tgatgaggtt ctaagtgttg gccagaagcg acagtttgca
     1741 gtcgagttga attcaggtgt gccgctggga ctggcgatgc tggcgttacg tttcaacccc
     1801 aaggtggtga aagtccatgc ggtttctccc ggtggactct tcgctgaggt aaaagacaaa
     1861 ggcccaagca ttactcagtc catcgacccg tctggtgtct gcttaatttc gatctccact
     1921 ctcaacggtg gtgcgccgat gagcggtacg ggcgcgttac tgctgatcga gatcgagggg
     1981 ctcacccctg gtgatgctgc gttggctttt gacagtgaaa caatgcacct tgtggccata
     2041 gatgcacgtg atgttgtgct tgaagtgaaa caaggacgca tgactgtgaa gcagtaaatc
     2101 gaatatcgat ggtgcgggaa cgcggcgacc atggtgagag cgattatatt tactggtcgc
     2161 cgcttctctt tgtcgaccgt tgagttcaaa tcaatgctga agcgaaaat
//
LOCUS       DASUEX010000448         2234 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1327909, whole genome shotgun sequence.
ACCESSION   DASUEX010000448 DASUEX010000000
VERSION     DASUEX010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1327909"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..921
                     /locus_tag="VFV61_07565"
     CDS             <1..921
                     /locus_tag="VFV61_07565"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HEX4900375.1"
                     /translation="RTLVLSAAVRNRYDIAKAFESHFRENFGYTLQMTAGGKDPLADF
                     LFRVKAGHCEYFSTAMAVMLRTEGIATRVVNGFLPGEYNGTADVFTVRQSDAHSWVEV
                     YFPETNAWVTFDPTPAAGRVEPQHAGFTAALGKYAEAFELLWFQYVVGYDRQEQRTLA
                     TSLHNQATRYQYTVAKAVDRLQRLSSPLWQQPVWALVLLALLAAIALLLIRFKRFGWR
                     GLKIRTRPVDSARSAIEFYERLTRLLIRKGFKRAPDQTPLEFATSAGLNEAVIITGAY
                     NRVRFGAEQLSSAELSQIEALLSKAEREEK"
     gene            926..>2234
                     /gene="rimO"
                     /locus_tag="VFV61_07570"
     CDS             926..>2234
                     /gene="rimO"
                     /locus_tag="VFV61_07570"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HEX4900376.1"
                     /translation="MNKVGFISLGCPKNLVDSEVMMGRLKASGYELTADASAADTIVV
                     NTCGFIDSAKQESIEAILEAAKLKTEGAATRLIVAGCLVERYRDELRAEMPEVDAFIG
                     TNQINDILSVCDPQTNTRALPVLPLGNQSATYLYDESTPRILATPQHYAFIKIAEGCD
                     RPCAFCFIPQMRGHFRSRRFGSIVAEAHQLAEEGVKELVLVAQDSSRYGEDLGKADAL
                     AGLLKELSRTEGIEWVRVMYTYPTHISDAFLDVLAGEPKAVKYLDMPLQHASQNVLKL
                     MKRGGNRESLERLIKRVRERVPGIGVRTTFITGFPGETEEDFAELLRFVQNVEFDRVG
                     VFTYSDEEGTPAYELPDKVDRKVAEKRRDRLMKEQSRISRRRHKAMVGQTVRVMFEGE
                     ANESELLWQGRMETQAPDIDGCVLINDAPEGFVPTPGEMVNVLI"
BASE COUNT          497 a          586 c          664 g          487 t
ORIGIN      
        1 cgaacgcttg ttctcagtgc cgctgttcgc aatcgctatg acattgccaa agcattcgag
       61 tcgcacttcc gcgagaactt tggctacacg ttgcagatga cggcaggggg taaggatcca
      121 ttggccgact ttctgtttcg cgtcaaagcc ggccactgcg aatacttctc gaccgcgatg
      181 gccgtgatgc tgcgcactga aggaattgct acgcgggtgg tgaatggttt cctgcccggc
      241 gagtacaacg gtacggctga cgtcttcaca gtaagacaaa gcgatgccca ttcctgggtc
      301 gaagtatatt tcccggagac gaatgcatgg gtgacttttg atccaacgcc cgccgccgga
      361 cgcgttgagc cgcaacacgc cggtttcacc gcagccctgg gcaagtacgc cgaagccttt
      421 gagcttttgt ggtttcagta tgtagttggc tatgaccgcc aggagcagcg aactctggca
      481 acttcactgc acaatcaagc cactcgttac caatacacag ttgccaaagc cgtcgatcgc
      541 ctacagcgtc tttcctcgcc cttgtggcaa caaccagtgt gggcgcttgt gttgctggcg
      601 ctgctggcgg caattgcgtt gttacttatt cgcttcaagc gctttggctg gcgcggcttg
      661 aagatcagga ctcgaccggt tgattcggcg cgatcggcaa tcgagtttta tgaacgtctg
      721 acgaggttat tgatcaggaa aggcttcaaa cgcgcgccgg atcaaacgcc gctcgagttt
      781 gcgaccagcg ccggactgaa cgaggctgtg atcatcaccg gcgcctacaa tcgggtgcgc
      841 tttggcgctg aacagctttc ctccgcggag ttgagccaaa ttgaagcctt attgagcaag
      901 gcggaacggg aagagaagtg aagcaatgaa caaagttggt tttatcagtc tgggctgccc
      961 caagaacctt gtcgacagcg aggtgatgat ggggcgccta aaggcaagtg gctatgaatt
     1021 aacagccgac gcctccgcag cggatacgat cgtggttaac acgtgtggct tcatcgattc
     1081 cgccaagcag gaatctatcg aagcgatttt ggaagccgcc aaattgaaaa cggaaggtgc
     1141 tgcgacgcgc ctgatcgttg ccggttgtct cgtcgaacgt tatcgcgatg agctcagagc
     1201 tgaaatgcca gaagtggatg cttttatcgg cacgaaccag atcaacgaca tcctgtcggt
     1261 ctgtgatcca cagacaaaca cccgcgccct tccggtccta cccttaggaa accagagcgc
     1321 aacttatctc tacgacgaat ccactccgcg catcctggcg acaccgcagc attacgcttt
     1381 catcaagatc gctgagggct gcgaccgtcc ctgcgccttc tgcttcattc cgcagatgcg
     1441 cggacacttc cgcagccgcc gctttggctc gatcgtggcg gaggcgcacc agttggcaga
     1501 ggaaggcgtc aaagaactgg ttctggtggc ccaggattcg tcccggtatg gtgaagacct
     1561 tggcaaggca gacgccctgg cgggcctttt gaaggaactc tcgcgcacag aaggcatcga
     1621 gtgggtacgc gtgatgtaca cctacccgac gcacatcagc gatgcgtttc tcgacgtgct
     1681 ggcaggagag ccgaaggcag ttaagtacct cgacatgcct ctgcaacatg cttcgcaaaa
     1741 cgtgctgaaa ctgatgaagc gcggcgggaa ccgagagagt ttggagagat tgatcaaacg
     1801 cgtccgggag cgcgtgccgg gaatcggtgt gcgcacgact tttatcaccg ggtttcccgg
     1861 cgaaaccgaa gaagacttcg ccgagttgct ccgctttgtc cagaacgtgg agtttgatcg
     1921 cgtcggtgtt tttacctatt cggatgaaga aggaactccg gcctacgagc tgcctgacaa
     1981 ggtggatcgc aaggtggcgg agaagcgacg tgatcggttg atgaaagagc agtcgcgcat
     2041 ctcgcgcagg cgtcacaagg cgatggttgg ccagactgtg cgcgtaatgt ttgaaggaga
     2101 agcaaacgag agcgagttac tctggcaggg acggatggaa acgcaggctc cggatatcga
     2161 tggttgcgta ttgatcaatg atgcgcctga aggttttgtt cctacaccgg gggagatggt
     2221 gaatgttttg atta
//
LOCUS       DASUEX010000449         4752 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1328035, whole genome shotgun sequence.
ACCESSION   DASUEX010000449 DASUEX010000000
VERSION     DASUEX010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4752
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1328035"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..624)
                     /locus_tag="VFV61_07575"
     CDS             complement(<1..624)
                     /locus_tag="VFV61_07575"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconeogenesis factor YvcK family protein"
                     /protein_id="HEX4900377.1"
                     /translation="MRSSSPKDRGLNLVAIGGGTGLSTLLAGLKRKVGRQETEDAWIE
                     NLSAIVTVSDDGGSSGRLREELQMLPPGDIRNCMIALSEDSNLLSRLFKHRFRGEGAL
                     GGHSFGNLFLAALTEVTGDFVEAVRLSSEVLASKGHIYPATINDVRLVGELEDGSIAQ
                     GETQITASLSKIRSLRLEPEQCLPLPEALVAIRNADLITVGPGSLYTS"
     gene            complement(731..1243)
                     /locus_tag="VFV61_07580"
     CDS             complement(731..1243)
                     /locus_tag="VFV61_07580"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX4900378.1"
                     /translation="MPPRRPPTVDESAALLAFEHAHKEFTNGHFGEARNQFRGLLEKY
                     SQVSEVAARARTYLAVAEARLRTENMLPRDADSLYDRGVIELNRGEYVAAQEMFERAL
                     KREPEAAHIHYGLAATRARLGSLDLALQSLQRALDLQPNLRVRAQHDHDLTSLRNDPD
                     FDKLIFQPRA"
     gene            1296..1682
                     /locus_tag="VFV61_07585"
     CDS             1296..1682
                     /locus_tag="VFV61_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900379.1"
                     /translation="MTLETKAFFGAAIGVLRAGACRGAAFRAGFSALTAFFFAGADVR
                     LLETFFAAVRGDAFCALRTGGFDDAFAFVRAGLAFKVRPLTDDLVAGRRAGVRDVERL
                     SPFVMGLLMCGEAFNLKDAIPPSAYP"
     gene            1992..2465
                     /locus_tag="VFV61_07590"
     CDS             1992..2465
                     /locus_tag="VFV61_07590"
                     /inference="COORDINATES: protein motif:HMM:NF015232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HEX4900380.1"
                     /translation="MPDTPILLREQQYHQIKAVLARLRMDAAAKVIFLVDKDGQEIAV
                     QGEVGNLDTTSLASLAAGNVAATGGMAQLIGEKEFPTLSHEGQRESIHISVIGRLLLV
                     VVFDDRSSLGLVKLRSKQVSQQISLMIKEIENADNHDLESPFAGITDADIDSLFS"
     gene            2472..3056
                     /locus_tag="VFV61_07595"
     CDS             2472..3056
                     /locus_tag="VFV61_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015347803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase domain-containing protein"
                     /protein_id="HEX4900381.1"
                     /translation="MTFINYASREINCKIVYYGPGLCGKTTNLQYIFDSTAPQSRGKL
                     ISLATETDRTLFFDFMPLELGTVRGFKTRFHLYTVPGQVFYDASRKLILKGVDGVVFV
                     ADSQEERMDANVESLYNLEENLRAQGYDPDKIPYVLQLNKRDLPNVLPIEDITAELRR
                     NGEPVIEAVASSGSGVFDTLKAVAKQVLTELRKG"
     gene            complement(3064..3618)
                     /locus_tag="VFV61_07600"
     CDS             complement(3064..3618)
                     /locus_tag="VFV61_07600"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEX4900382.1"
                     /translation="MAVLKKIRTRWWSPAVPPEPFPVQPERAIAPASGIRYDVRPLTI
                     SQLDECWRLDQRCFVDGEAYSRETFEYLLTAAESVSYRAVTPGGVMVGFVIGLVEPDH
                     TGHVTTVGVAPEHRRRHLAKRLMLQVEDGFKRRNVRLVRLEVRSLNTAAQRVYETLGY
                     TVTQRLPKYYSNGGDGLLMLKSLD"
     gene            complement(3672..>4752)
                     /gene="queA"
                     /locus_tag="VFV61_07605"
     CDS             complement(3672..>4752)
                     /gene="queA"
                     /locus_tag="VFV61_07605"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEX4900383.1"
                     /translation="PNLSTELALVKATAMHVSAFDYKLPSELIAQEPLPQRDASRMLI
                     VDRQNQSWTDSQFRELPQQLRPTDLLVINNTRVFPARLSGHREPSGGRVEILLVRELE
                     TDVWEALVKPGRRLRKGAEVIFDGAHLTAQLLDQPGKELRQLRLQSLNPLSSVLDEFG
                     EIPLPPYISRPSGSSALDRERYQTLFAKARGAIAAPTAGLHFTPEVLAEITARGTRLA
                     EITLHVGYGTFEPVRVGDTEQHQVAAERYQIDDSAAQAINETRARGGRVIAVGTTTTR
                     ALESAVNDSGNLNATQGSATLTIVPGYKFRVVDALLTNFHLPCSSLLMLVSAFAGHQL
                     ILGAYRHAVASRYRFYSYGDCMLVI"
BASE COUNT         1063 a         1276 c         1331 g         1082 t
ORIGIN      
        1 gctggtgtaa agcgagccag gcccaactgt aatcaggtca gcatttctaa ttgctaccag
       61 ggcctcaggg agtggcagac attgttcggg ctccaggcgc agggaacgaa tcttcgaaag
      121 cgacgcagtg atctgtgttt caccctgagc gattgaaccg tcctcgagtt ctcccacgag
      181 gcgcacgtcg ttaatcgtcg cgggataaat atgtcccttg ctggcgagca cttccgaaga
      241 caagcgcacg gcttctacaa aatcgccggt tacttcggtt agtgcggcaa gaaatagatt
      301 tccaaaactg tgaccgccga gtgcgccttc accccggaaa cgatgcttaa agagccggga
      361 aagtaaattc gaatcttctg acagcgcgat catgcagttg cgaatatcgc ccggtggcag
      421 catctgcaac tcctcgcgca gacggccgga actaccgccg tcatcagaaa cggtcacaat
      481 tgcggagaga ttctcgatcc acgcgtcttc ggtctcctga cgcccaacct tgcgcttcaa
      541 gccggcaagc agcgttgaga gaccggtccc gccaccgatt gccaccaggt tcagaccacg
      601 atcctttggg gagctagaac gcaattcctg aatctccacg cctcacgggc gacagggaat
      661 gatacctaaa acagaaaagc tttcaacttg tctttcgcag cgattctctg gggaagggga
      721 tggccagagg tcaggcacgg ggttggaaga tcaacttatc gaagtccggg tcattacgca
      781 gtgaggtcag atcatgatcg tgctgcgcac gaactcggag gtttggttgg agatccaagg
      841 ctcgttgaag agattgcaaa gccagatcaa gtgaaccaag gcgagcacga gtcgccgcca
      901 ggccataatg aatgtgggcc gcttcgggtt cacgcttgag ggcccgctca aacatttcct
      961 gcgctgcaac atattcaccg cgattcagtt cgattacacc ccgatcgtac agagagtcag
     1021 catcgcgcgg gagcatgttc tcggtgcgca aacgtgcttc agctacagca aggtaggtac
     1081 gagcgcgtgc ggcaacttcg gaaacctggg aatacttttc cagaagtccg cggaattgat
     1141 tacgggcttc tccgaagtga ccattcgtaa attctttgtg ggcgtgttcg aacgcgagta
     1201 aggccgccga ttcatcgact gttggcggac gtcgaggtgg caccggagca acgatgggac
     1261 gttgtgcacg aacggcagca agcttctgag tctttttgac tctcgaaact aaggctttct
     1321 ttggtgctgc aatcggggtc ttacgtgcgg gagcttgccg aggtgcagcc ttccgggcgg
     1381 gctttagtgc tttgactgct ttctttttcg cgggtgccga tgttcgcttg cttgaaactt
     1441 tttttgcggc cgtccgtgga gatgccttct gcgctttgcg gactggcggc tttgatgatg
     1501 cctttgcttt tgttcgagcc ggtttggctt tcaaagtgcg ccctttgaca gacgatttgg
     1561 tcgcaggtcg tcgcgccggt gtgcgcgatg tcgaacgcct tagtcctttt gtcatggggc
     1621 tgctcatgtg tggggaagct ttcaatttga aggacgctat tccgccctcc gcgtaccctt
     1681 gagaacgggc cagagtttaa cacagcctct cctgtaaatc aacgcggaaa agggagttgc
     1741 ggcgatattc gctggtcgac gcgctgaaaa tgtcgtgtca tggccaacca agaaaaacac
     1801 ttgtaggtca ttgttggcta tgttagagtg cccaaggctc agcaaagccg agccgctccg
     1861 ctaagttcgc atcgcaactc ttgctggaaa attcctaggc agtcaacatc ttgcctcaag
     1921 aagtgcttcg ggtctccagc caacaacctc ccgatagtct tgcccacctt ttgccgaaga
     1981 aagctgtatt gatgccagac actccgatcc tgctgcggga gcagcagtac caccaaatca
     2041 aggctgtctt ggcgcggctc cgaatggacg cggcagccaa agtcattttc ctggttgata
     2101 aggatggtca ggaaatcgcc gttcagggag aagtgggaaa tcttgacaca accagcctcg
     2161 cctcgctggc cgcgggcaat gtcgcggcga ctgggggcat ggctcagttg attggcgaga
     2221 aggaatttcc cacgctttca cacgagggac agcgagagag cattcacatc tccgtcattg
     2281 gccggcttct gctcgtcgta gtcttcgatg atcgctccag cctcggtttg gtaaagctcc
     2341 ggagcaaaca ggtttctcaa cagatctcgt tgatgattaa agagattgag aacgccgata
     2401 atcacgatct cgaatcacca tttgccggaa tcaccgacgc ggatattgac agccttttca
     2461 gttaagaagc catgactttc atcaactacg catcgcggga aatcaactgc aagatcgtct
     2521 actacggccc aggtctctgt ggtaagacga ccaacctaca gtacattttt gattcaacgg
     2581 cccctcagtc cagaggcaag ctgatcagcc tcgcgacgga aactgatcgc acgctcttct
     2641 ttgattttat gccgttggaa ctcggaactg ttcgcggttt taagacgagg ttccatctct
     2701 atacggtgcc cggacaggtt ttttacgatg cttcgagaaa gcttattcta aaaggcgtgg
     2761 acggagtggt ctttgtcgcc gactcgcagg aagagcggat ggacgcaaat gtagagtcgc
     2821 tctacaacct cgaggaaaat ctccgggcgc aaggatacga ccctgataag attccctatg
     2881 tgctccagct taataagcga gatttgccaa acgtgctccc gatcgaagac ataaccgcgg
     2941 aattgcgccg aaacggcgag ccagtaatcg aagctgttgc atcttcaggt tccggcgtct
     3001 tcgatactct aaaggcggta gctaagcagg tcttgactga gttgcggaag gggtagacac
     3061 gttctagtca agggatttga gcatcagcag gccgtcaccg ccatttgaat aatattttgg
     3121 cagccgctgc gtgaccgtat accccagggt ttcatagact ctctgtgccg ccgtattgag
     3181 agatctcacc tccaggcgaa ccaacctcac gtttcgccgc ttgaatccat cttctacttg
     3241 cagcattaag cgcttcgcga gatgccgccg acggtgttcc ggcgctactc cgacggtggt
     3301 gacgtgcccc gtatgatctg gctctacgag tccgatcaca aaacccacca tcacgcctcc
     3361 gggcgtaacc gcgcgatagg aaactgattc cgctgcagtg aggaggtatt caaacgtctc
     3421 ccggctgtag gcttcgccat cgacgaagca gcgctgatcc aggcgccagc attcatccaa
     3481 ttgcgagatt gtgagcggac gtacgtcgta ccggattccg gacgcagggg cgatagcccg
     3541 ctccggttga actggaaatg gctccggagg aaccgcgggt gaccaccaac gtgtgcgaat
     3601 tttctttagt actgccattg agatgacgac acttcgtcgc ggctagttta gcaccatagt
     3661 tttccggatc actagatcac aagcatgcag tcgccgtagc tataaaaccg ataacgagag
     3721 gcgacagcgt gacgataagc gccgagtatt agttgatgac cggcgaatgc gctcaccagc
     3781 atgagcaagg acgaacaggg cagatgaaag ttcgttaaga gcgcgtcaac gacgcgaaac
     3841 ttgtagcctg gaacaatggt caacgtggcg cttccctggg ttgcgttcag gttgccgcta
     3901 tcattcaccg ccgactcaag cgctcgtgtt gtcgtcgtcc ctaccgcaat cacccgcccg
     3961 cctcgggctc gtgtttcgtt gattgcctga gccgcgctat cgtcaatctg gtatcgctcc
     4021 gctgcaactt gatgttgttc ggtatcgccc acgcgcactg gctcaaaagt tccatagcca
     4081 acgtgaagcg ttatctcggc gagtctcgtg ccgcgtgcag tgatttcagc caggacttcg
     4141 ggcgtgaaat gcaatcccgc agtcggtgcg gcaatggccc cacgcgcctt cgcaaacagc
     4201 gtttgataac gttctcgatc caaagcggag ctgcctgagg gtcggctgat gtaaggaggc
     4261 aaagggattt cgccaaactc gtccagaacc gaactcaaag ggttgagcga ttgcaagcgc
     4321 agttgtcgca attccttgcc tggctgatcg agtagttgcg ccgtcaaatg cgccccgtca
     4381 aagatcacct cagcgccctt gcgcagccgg cgaccaggtt tgacaagcgc ttcccaaaca
     4441 tctgtttcca gctcacgcac caggagaatt tctacgcgac cgccggaagg ctcgcggtgc
     4501 ccgctgaggc gagcggggaa gacgcgtgtg ttgttgatta cgagaagatc ggtgggcctc
     4561 agttgttgcg gcaattcgcg gaattgcgag tcagtccaac tctgattctg tcgatcgact
     4621 atgagcatgc gcgaggcgtc gcgttgcgga agcggttcct gcgcgatcag ctctgatggc
     4681 aacttatagt caaaggcgga gacatgcatt gcggtggctt taacgagagc aagttcagtc
     4741 gacaggtttg gg
//
LOCUS       DASUEX010000450         2433 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1328407, whole genome shotgun sequence.
ACCESSION   DASUEX010000450 DASUEX010000000
VERSION     DASUEX010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1328407"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..533
                     /gene="pstA"
                     /locus_tag="VFV61_07610"
     CDS             <1..533
                     /gene="pstA"
                     /locus_tag="VFV61_07610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEX4900384.1"
                     /translation="SLVRFMVDTLTGIPSIVTGVFVYTIVVLPLRHFSALAGGIALAM
                     IMIPIVARTTEEMIRLVPHSLREGALALGAPQWRVTLGVVVPAAASGIATGAMLAIAR
                     ISGETAPLLFTAFGSRFFSYYLDQPIASLTVQIYNYAISPYDEWHAQAWAATLVLMTL
                     ILSINIAVRFLTRKKF"
     gene            536..1366
                     /gene="pstB"
                     /locus_tag="VFV61_07615"
     CDS             536..1366
                     /gene="pstB"
                     /locus_tag="VFV61_07615"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HEX4900385.1"
                     /translation="MSLPTPTIKTTVSAFADPSRSISNAATKIAVKDLDFYYGKTQAL
                     HEISLTIPERVVMAFIGPSGCGKSTFLRTLNRMNDVIPGTRVAGKVLIDGRDIYAPGT
                     DVVELRRKVGMVFQKSNPFPKSIFDNVAYGLRINRLTSSNTELAERVEQALLDAALWE
                     EVKDRLKSSALSLSGGQQQRLCIARALAIRPEVVLMDEPASALDPVATYKIEELIVEL
                     KKQYTIVIVTHNMQQAARVSDTTAFFLLGKLIEVNPTEKMFTNPEQKLTEDYITGRFG
                     "
     gene            1492..2151
                     /gene="phoU"
                     /locus_tag="VFV61_07620"
     CDS             1492..2151
                     /gene="phoU"
                     /locus_tag="VFV61_07620"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEX4900386.1"
                     /translation="MEHHRHLDDELNSLRDRVLLLGGEVEAALQKGMTALLERDSELA
                     RSVLDHDDAIDSIEVEIDRQCIDIIALRQPAARDLRFVISVAKMAPVLERIADHACNI
                     ARAAIDLNNEPELKQTDELRLMADEASSMLHAALDAFTTNDARAAREVIKRDSEINER
                     YNQIFHSLIEKMVSEPATATRNARLIFIAKHLERVGDYVTDICELTVYMAEAAFIKHS
                     N"
     gene            2156..>2433
                     /locus_tag="VFV61_07625"
     CDS             2156..>2433
                     /locus_tag="VFV61_07625"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX4900387.1"
                     /translation="MARPVLIIEDDPDISESLKYNLEREGLNTIVAATGEQGLSEALN
                     ESNPPLLIMLDLMLPGMSGTELCRRFRREPLTRRTPIIMLTAKGSESDR"
BASE COUNT          568 a          635 c          662 g          568 t
ORIGIN      
        1 gcagcctcgt tcgttttatg gtcgacacac tgactggcat tccttcgatc gtaacgggag
       61 tattcgtcta cacgattgta gttttaccgc tgagacactt ctctgcgttg gctggaggaa
      121 ttgcgctcgc aatgatcatg attccgattg tagcgcgcac tactgaggaa atgattcgtc
      181 tggtgcccca cagcttgcgc gaaggcgccc tggcgctcgg tgcgccgcaa tggcgagtga
      241 cgttgggagt tgtggtgcca gccgcagctt ctggaattgc gacgggagca atgctggcaa
      301 tcgccagaat ttccggggag acggcgccgt tgttgtttac cgccttcggc agcaggttct
      361 tcagttacta cctggaccaa ccaatcgcct cgctgaccgt gcagatttat aactacgcga
      421 tttcgccata cgatgaatgg catgcacagg cttgggctgc gaccctcgtg cttatgactt
      481 tgattttgtc gatcaatatt gcggtgcggt tcttgacccg taagaaattc tagcgatgtc
      541 tttgcctact ccgacaatca aaacgacagt cagtgccttt gccgatccct cgcgcagcat
      601 cagcaacgct gccaccaaga tcgccgtcaa ggatttggat ttctactacg gtaagactca
      661 ggccctgcat gagatctcat tgaccattcc cgaacgagtg gtgatggcct tcatcggccc
      721 gtctggttgc ggcaagtcca catttttgcg cacgctcaat cgcatgaacg acgtgattcc
      781 gggcacacgc gtcgcgggca aagtgctaat tgatggccgg gacatttacg cgcctggcac
      841 tgacgtagtg gagctgcgcc gcaaggtggg catggtgttt cagaaatcga atccgtttcc
      901 caagtcgatc tttgataacg tggcgtatgg gttaagaata aaccggttga cttccagcaa
      961 tacggaactt gccgaacgag tcgagcaagc gctgctggat gccgcgctct gggaagaagt
     1021 caaagatcgc ttgaagtcct cggcgcttag cctttccggc ggtcagcaac agcgactctg
     1081 tatcgcgcgg gccctggcca tcagaccgga agttgttttg atggatgaac cggcatcagc
     1141 gctcgatccg gtagcgacct acaagatcga agaactcata gtggaactaa aaaagcagta
     1201 cacgattgtg atcgttaccc acaacatgca acaagcagcg cgcgtttccg acacgacggc
     1261 gttcttcctt cttggtaagc tgatcgaagt gaaccccacg gaaaagatgt ttaccaatcc
     1321 ggaacagaag ctgacggagg attacattac agggaggttt ggctgacaag attgccgatt
     1381 ggcgattgcc aattgccaat cggcaggaat cccgatcata agttgttatt gaagcgcgct
     1441 ctggaatcgg tacgtaatag gcaatccgca atcgccaatc gccattaata tatggaacat
     1501 catcgacatc ttgatgacga actaaattcg ctccgcgacc gcgttttact gctcggtgga
     1561 gaggttgagg cagcgttgca aaagggcatg accgccctgc tggaacgtga cagtgagctc
     1621 gcgcgcagcg ttttggacca cgacgacgcg atcgacagca ttgaagtcga gatagatcgg
     1681 cagtgcatcg atatcatcgc cttgcggcag ccggccgcgc gcgaccttcg tttcgtaatt
     1741 tcagttgcga aaatggcgcc ggtgctggaa cgtatcgccg atcatgcgtg caatatcgcc
     1801 cgcgcggcca tcgacctcaa caacgagccg gaactaaaac agacggacga gttgcgttta
     1861 atggcagacg aggcgtcttc tatgttgcat gcagccctcg atgcttttac tactaatgat
     1921 gctcgcgccg cgcgtgaggt gatcaaacgc gattctgaaa tcaatgagcg ttacaaccag
     1981 atcttccaca gtttgattga gaagatggtt tccgaaccgg caacggctac ccgcaacgcc
     2041 cggcttatct ttatcgccaa acacctggaa cgtgttggcg attacgttac cgatatttgc
     2101 gagttaaccg tgtacatggc tgaggcggcg tttatcaaac acagtaacta atgtaatggc
     2161 gcgtcctgtt ctgataattg aagacgatcc ggacatctcc gagagtttga aatacaacct
     2221 cgagcgtgaa ggtctaaaca cgattgttgc tgccaccgga gaacaaggtc ttagcgaagc
     2281 tcttaacgaa agcaacccgc cgctgttgat catgctcgat ctgatgctgc cgggtatgag
     2341 tggcaccgag ctctgtcgcc gttttcgccg cgagccacta acgcgacgca cgccgattat
     2401 tatgttgacg gccaaaggtt cggaatcgga tcg
//
LOCUS       DASUEX010000451         2351 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1576239, whole genome shotgun sequence.
ACCESSION   DASUEX010000451 DASUEX010000000
VERSION     DASUEX010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1576239"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="VFV61_07630"
     CDS             <1..204
                     /locus_tag="VFV61_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012536746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900388.1"
                     /translation="DHTSATELLTLPHPRMHLRRFVLLPLAELVPDLVHPTLQQTIAT
                     LLENLDDQSEVKHWVPNPEREKR"
     gene            602..1495
                     /gene="panB"
                     /locus_tag="VFV61_07635"
     CDS             602..1495
                     /gene="panB"
                     /locus_tag="VFV61_07635"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: protein motif:HMM:NF001452.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEX4900389.1"
                     /translation="MPYLKPERPEKVSAPSLRASKQRGERLVCLTAYDYPTARIVDEA
                     GIDIVLVGDSLGNVVLGYGNTVPVTLDEIMIHLKAVRRAVQRALLVADMPYGSFHTGA
                     DDAVKNALRFVKEGGAEAIKLEGGRKRLPLIKRLVDEEIAVMGHIGLTPQSINKLGAY
                     RVQGKTAEAARELLDDALALEEAGAFAIVLEVVPREIAQLITKSVSIPTIGIGAGAGC
                     DIQVLVLHDMLGLSFGKLARFVRPYANLHQTITDAVTRYADDVRNGTYPSDAESYALP
                     AEAAAELHLSAPVKETKLKKS"
     gene            1512..>2351
                     /gene="panC"
                     /locus_tag="VFV61_07640"
     CDS             1512..>2351
                     /gene="panC"
                     /locus_tag="VFV61_07640"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF014611.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEX4900390.1"
                     /translation="MEIINRRQRMSSVARKIRREQDRTIGLVPTMGALHEGHLSLVRE
                     ARRMCDVVVVSVFVNPTQFGPGEDFERYPRDLTRDTALLTDYNVDYIFAPPVEEIYPK
                     GFTTYVNVSGLSKLFEGESRPGHFRGVATIVTILFNIVKPDFAFFGQKDAQQAVIIRR
                     LVKDLGLDTEVLVMPTVREDSGLAMSSRNSYLSAEEQAAATVIHRALVKAKESFKAGE
                     RNAGKLADQVKTTIESEPRVRLDYVGIVDAETLERLDKLDERPILIAVAAFVGKIRLI
                     DNIT"
BASE COUNT          533 a          628 c          652 g          538 t
ORIGIN      
        1 gatcacacat cggctacgga acttctaacg cttccccatc cacgcatgca tctgcgacgc
       61 tttgtcttac tgccgcttgc agaactggta cccgaccttg ttcatcccac gttgcaacaa
      121 acaatcgcaa ccctattgga aaacctggat gaccaatctg aggtcaaaca ctgggtgcct
      181 aaccccgaga gggagaagcg gtgacgggga gatggggtga aggggtgaag atgatgggca
      241 gtaggcaaaa acattttcca tatgtcattt tccatttccc attttccatt ttattgaagc
      301 aagggagtcc cgcagttcag cgggcactgt ttcctacgcc gggccaatat ccttagataa
      361 tgggctgttc tgtaaccgat caaaatgaaa aatgggaaat gacatatgga aaatgtctct
      421 ggtctactgc cctcatcttc cccctttccc caagtcccag catcaccgct tctccgtttc
      481 tccctttctc ccggatttgc cgtataatgc ctgccttgct agcaagccct aacatcccca
      541 aaactgtagt ctggttctgc ggtcaatttg cacccagacg atcttgatag agagctgaat
      601 catgccgtat ctcaaacctg agcgtccgga aaaagtcagt gcgccttcat tgcgagccag
      661 caaacagcgg ggcgagcgtc tggtttgtct aactgcttac gattatccga ctgcgcgcat
      721 cgtcgatgag gcgggtattg atatcgtgct cgttggcgat agcctcggca atgtcgtgct
      781 cggctacggc aacacggtac cggtcaccct ggacgagatt atgattcacc tgaaggcggt
      841 gcgacgcgcg gttcagcgag ccttgctggt ggccgatatg ccctacggtt catttcatac
      901 cggcgctgac gatgccgtga agaatgcttt acggttcgtc aaggaaggcg gcgccgaggc
      961 catcaaactc gaaggcggtc gcaaacggct acctctaatc aagcggctgg ttgatgaaga
     1021 gatcgctgta atgggtcaca tcggcttaac gcctcaatca attaacaagc tgggcgcata
     1081 tcgggtccag ggcaagaccg ccgaagctgc acgtgaactg cttgacgacg cgcttgcgct
     1141 cgaagaagcg ggcgccttcg caatcgttct ggaagttgtg ccgcgagaga tcgcccaact
     1201 gatcacaaag agcgtcagca ttcccactat cggcatcggc gccggtgccg gttgcgatat
     1261 tcaggtcctc gtgctgcacg acatgctcgg tctttcattt ggaaaacttg ctcgctttgt
     1321 ccgtccttac gcgaacctgc atcagaccat taccgacgca gttacgcgct atgccgacga
     1381 cgtgcgcaat ggcacctatc cttcggatgc cgagtcgtat gcgttgccgg ccgaagccgc
     1441 ggcagagttg cacctgagcg cgccggtgaa agagacgaag ctcaagaagt cctgaagttt
     1501 ccccaaaaac tatggagatc attaatcgtc gtcagcgcat gagttccgtc gcgcgcaaaa
     1561 tccggcgcga acaggatcgg accatcgggc tggtaccaac gatgggagcg ctgcatgaag
     1621 ggcacctgag tctggtgcgc gaagcgcggc ggatgtgtga cgtcgtcgtg gtttcggtgt
     1681 ttgttaaccc gacgcagttt ggaccgggcg aggattttga acgctacccg cgcgatctga
     1741 ctagagacac ggcgctcctg actgactaca acgttgatta catttttgcg ccgcccgtcg
     1801 aagagattta cccgaagggt ttcaccactt acgtaaacgt cagcggactg tcgaagctgt
     1861 tcgaaggtga atcgcgcccg ggacattttc gtggggtcgc gacgatagtc acaatcttat
     1921 tcaacatcgt gaagccagac tttgctttct ttggtcagaa ggacgcgcaa caggctgtga
     1981 tcattcgccg gctcgtgaaa gatctggggt tggacaccga ggtcttggtg atgccgactg
     2041 tgcgtgaaga ttccggtttg gcaatgtcct ctcggaattc ttatctttcg gcggaagagc
     2101 aggcggcggc taccgtgatt catcgagcgc tggtgaaggc caaggaatca tttaaggcgg
     2161 gcgagcgtaa tgcgggcaaa ctcgcggatc aagtgaagac cacgatcgaa agcgagcccc
     2221 gtgtgcgttt ggattacgta ggtattgtcg acgcagaaac actggaacga ctcgataaac
     2281 ttgatgagcg cccaatcctg atcgcggtag cggcgtttgt cggcaaaatc cgcttgattg
     2341 ataacatcac c
//
LOCUS       DASUEX010000452         6076 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_999881, whole genome shotgun sequence.
ACCESSION   DASUEX010000452 DASUEX010000000
VERSION     DASUEX010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6076
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_999881"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            310..1269
                     /locus_tag="VFV61_07645"
     CDS             310..1269
                     /locus_tag="VFV61_07645"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX4900391.1"
                     /translation="MHFPFQLPAKKEFDAVGFGLNAVDHLVVVPAYPDFDTKIRLVEH
                     RRSAGGQTATAMVALQRLGLRTAYAGRFGADDEGEFGFASVRDESVNVEFAEVVTGAR
                     NQIAFILIDQRNGERTIIWDRDERLAYKPSEAPIALASRGRVLHLDAHDPLACIPMAH
                     AAHEANAIVSADIDNAYEGLLELLPSIDILITSRELPHRLTGIADERASLVEMKARYG
                     CALVGMTMGKRGAVLYCEGQFIEAPAYEVPGGCRDTTGAGDAFHAGFLYGLLRGEEID
                     TSMRLANATAALKCRDLGARTALPNAAELQGFLRKGKVMVHPA"
     gene            1615..3255
                     /locus_tag="VFV61_07650"
     CDS             1615..3255
                     /locus_tag="VFV61_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900392.1"
                     /translation="MKLIARFLTVILLALFCFAVADAQEMHTHRHDPSEKLGRVNFPV
                     SCNAAAQRQFNRATALLHSFWYEEAEKTYTAITRMQPKCSMAYWGVAMSLYHPVWAPA
                     TPAELQKGWAAVEKAKTVGEITVRERDYIAAIEAFYRDSGALDHRNRALAYEKAMERV
                     SLSHPKDREAEIFYALALLGTALNTDKTYANQKKAADILNRVLSEEPDHPGVAHYLIH
                     SFDYPQLATLALPAARSYAKIAPSSPHALHMPSHIFTRLGLWQESIQSNLASAAIAKK
                     HVAISHPGAASFDELHALDYLVYAYLQGAEDQKSRQILVQVAALKKVDAPVVAAAYAF
                     TAIPARYALERRRWSEAARLELHPASFPWDHFRYAEALIYYARAVGAARSGNPASARK
                     DVEKLSAIQSAITEPRGSYWANQAEVERRTANAWLALAEGNKAEALTLMRSAADLEAT
                     TEKHPVTPGPIIPARELLGDMLIELDQPEQALREFEGSLGDSPNRFNGLFGAARAAEL
                     SGDKKKAGDYYAKIVTLTANADSDRSELQKAKKFLAQK"
     gene            3476..3661
                     /locus_tag="VFV61_07655"
     CDS             3476..3661
                     /locus_tag="VFV61_07655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900393.1"
                     /translation="MYTLRHTGISLMLWAGVDVVTVARITGTSVAMIDATYGHIIEAL
                     QRTAIDRVTALLAVAQR"
     gene            complement(3905..3981)
                     /locus_tag="VFV61_07660"
     tRNA            complement(3905..3981)
                     /locus_tag="VFV61_07660"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3945..3947),aa:Arg,seq:acg)
     gene            complement(4181..5209)
                     /locus_tag="VFV61_07665"
     CDS             complement(4181..5209)
                     /locus_tag="VFV61_07665"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX4900394.1"
                     /translation="MISAKLYQNSIRRLLLVFGLAFVCGSFSISQAQEPDDVVRTSTS
                     LVQLNVGVVDSRGRAITSLSRNDFSIYEDGVKQTIFQFEPTNAPFSVVLLLDMSGSTL
                     SFREQLKLATTRFLDALAPEDRVAVVQFNAKVKRLANFDASRKDTAYAIQIAEGAGET
                     HLYEALTYALKELQKEEKRRKAIVVLTDGLDTQMRNVDRAAVARSQTDEEALNAIKPN
                     ASASLNAVLNLADRQGVTIFPLALPSGDPKRLPIPGPDITGIYAAARTRLESIANRTG
                     GRLTEINRLDQMARLYAEVAANLRTLYTVAYQPPGDRPRDGKWHEIRVEVVHPELIAR
                     TRPGYFAR"
     gene            5407..5850
                     /locus_tag="VFV61_07670"
     CDS             5407..5850
                     /locus_tag="VFV61_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900395.1"
                     /translation="MYRPNFCSECGTKIVRLHWHPWTSRRFCDGCARRLRKERLRPPL
                     LAACLLLSIGFATGRALRPKPPPLIIQRSASSPLAKSESPGLHGQSGVNANSQTPPVE
                     EVVYICGARTKKGTPCSRRVHQPGRCWQHKGRPAMLPEEKLIVKD"
BASE COUNT         1386 a         1625 c         1702 g         1363 t
ORIGIN      
        1 atagacgcca gccgaacgcg gaatgttttt gagtttttct tctaaaggca ctatgcaaac
       61 attctagctg acaacaagag cgcaggagaa agcgcttaga tggtagacag tagatggtag
      121 tcggtaggag aagcatacgg cagcgcaaaa cgcccgcaat gctcagtccg gaagggcggt
      181 ttacccccgc tgcttgaatg gcattcaacc ttagcggggg caagcccacc ttccggactg
      241 agcatatccg acggggccta gacaaagttc acagatcaaa aaagaaccaa agaccaaaaa
      301 ccattcccga tgcattttcc ttttcaactc ccagcaaaaa aagagttcga tgccgtgggc
      361 tttggcttga atgctgtcga tcatctggtt gtagtgccgg cctatcctga ctttgataca
      421 aagattcggc tggtcgaaca ccgccgctcg gccggggggc agacggcgac cgccatggtt
      481 gcactgcaac gcctgggtct gcgcaccgct tacgccggca ggtttggtgc agatgatgaa
      541 ggtgaattcg gatttgcctc cgttcgtgac gaaagcgtaa acgtcgagtt tgccgaagtg
      601 gtgaccggtg cgcgtaacca aattgcgttc attctgatcg atcaacggaa tggtgaacgc
      661 acgatcatct gggatcgcga tgaacggctc gcttacaaac cgagtgaagc gcccattgcc
      721 ttggcctcgc gcgggcgagt acttcatctc gacgcgcacg atccgcttgc ctgcattccc
      781 atggcgcacg cggcgcacga agcgaacgcg attgtctcag cggacatcga caacgcttac
      841 gaggggttgc tggagttgct accgtcgatc gacattctga tcacttctcg agaattacct
      901 catcgtctga ccggaatcgc tgacgagcga gcttcacttg tcgagatgaa agcccgctat
      961 ggatgtgcgc tggtcggaat gacgatggga aaacgcggcg ctgtgctgta ttgcgaaggc
     1021 caattcatag aagcgcccgc gtatgaagtg ccgggtggct gtcgcgacac cacgggcgcc
     1081 ggggacgcgt tccacgcggg ctttctttac ggtttgctgc ggggtgaaga gattgacact
     1141 tcaatgcgcc tcgcgaatgc cacggccgca ctcaagtgca gagatcttgg ggcaagaaca
     1201 gcattgccaa acgcggctga gttacaggga tttttgcgta aggggaaggt aatggtgcac
     1261 ccggcatgat tcgaacatgc gaccccctga ttcgtagtca gtcataatcc gttttggtgg
     1321 gtttcactgg ttgtgggtag ttgtggtttg ttacgcgttt ccgcattgga atcgttgcgt
     1381 tcgctaacag tagttttcag tagttgcgtg tggttcgtac accatcggta cactggaatt
     1441 ggaccagcga ggcatcagaa tcagcactgc tcggaaaaac tcatctcttt tttgtgttgt
     1501 cgggctgcgt gacgaaggat tcaattgagt tttggactcg cggtggatag catacagttc
     1561 caacgagacg ggcccacgag aattatgatt gaccatttga aaaggagatt ggcgatgaag
     1621 ctcattgctc ggtttctcac tgtgatcctc ctggcgcttt tctgctttgc cgtcgccgac
     1681 gcgcaagaga tgcatacaca tcggcatgac ccttcggaaa aactcggacg ggttaacttt
     1741 cctgtctcct gcaacgctgc ggcccagagg caattcaatc gcgccacggc cctgctgcac
     1801 tctttttggt atgaagaagc cgaaaagacc tataccgcta ttaccaggat gcagccgaaa
     1861 tgctcgatgg cctactgggg agtcgccatg agcctttacc atcccgtgtg ggcgcccgcc
     1921 actcctgcgg aattgcagaa aggttgggct gctgttgaaa aagcaaagac tgtaggagag
     1981 ataaccgttc gtgaaagaga ctacatagcg gcgatagaag ctttctacag agattccggc
     2041 gcgctcgacc accgaaaccg cgctcttgcc tacgagaaag ccatggaacg ggtctccctc
     2101 agtcatccga aggatcgaga ggcagagatt ttctatgcgc tcgcgcttct tggaacagct
     2161 ctcaacaccg acaagactta cgcaaatcaa aagaaagctg ctgatattct aaatagggtt
     2221 ttgtccgagg agccagacca tcccggtgtg gcccactatc tgatccatag tttcgactat
     2281 ccgcagcttg caactctcgc gctgcccgcc gctcgcagct acgctaagat tgctccttcg
     2341 tctccacacg cgcttcatat gccctcacac attttcacgc ggctcggact gtggcaggaa
     2401 tcaattcaat cgaacctcgc ctcggcggcg attgctaaga aacatgtagc catatctcat
     2461 ccaggcgccg cctctttcga tgagctacac gctttggatt accttgtgta tgcgtatcta
     2521 caaggcgctg aggaccagaa atccaggcaa atactcgttc aagtcgccgc tctcaagaag
     2581 gtcgacgccc cagttgtagc ggctgcttac gcttttaccg ctataccagc gcgctatgcg
     2641 ctggaaagac gtcgctggtc tgaggctgct aggcttgagt tgcacccggc gagttttccc
     2701 tgggatcact ttcgttatgc cgaagccctg atctattacg cgcgtgcggt cggcgcagcg
     2761 cggagtggta atcccgcctc cgctcgcaag gatgtggaaa agctatccgc gattcagagc
     2821 gcaatcaccg agcccagagg aagctattgg gctaatcaag ccgaagtaga gcggcgaacg
     2881 gcgaacgcct ggcttgcgct cgcggaagga aacaaagcgg aggcgttgac gttaatgcgc
     2941 tcggctgccg acctcgaggc tacgacagag aagcatccgg ttacgccggg ccccatcata
     3001 cccgcgcgcg agttgttagg agacatgctc atcgaactcg atcaaccgga gcaggcgttg
     3061 cgagagtttg agggttcact gggggattca ccgaaccggt tcaatggtct ttttggcgcc
     3121 gctcgagcgg cggaactttc gggtgataag aagaaagctg gcgattacta cgcaaagatt
     3181 gtgactctta ccgctaacgc agattcggac agaagtgagc tgcagaaggc aaagaagttt
     3241 ttggctcaga aatagaacac gtgtaaggaa gccagaagag atcgcgggat cgagggccaa
     3301 ttcttgcgag ctaaaggcac actctccgac gcagtgctcg ttgatatggc gtccccggca
     3361 ctcaactccg agaacgcccc tgaaaacagg aacgccgctg tcgtggaaga gccttaagcg
     3421 ccgccacctg aaaccactgg taaaagctgc gggaatctcc gaggagggca tcagcatgta
     3481 cacattgcga cacacaggca tcagtttgat gctgtgggca ggtgtggatg tggtcactgt
     3541 tgcccggatt acaggcactt cggttgctat gattgatgcc acctacggcc acatcatcga
     3601 ggccctgcaa cgaacggcaa ttgaccgggt cactgcgctg ctggctgtag cgcagcgcta
     3661 aaggggaagc ctctctgttc ccagcttttt tgtattttca ccaccgtcgc tcaatggagg
     3721 cccgcacttc ctcagccccc gaactcgaac tcccagttgg ggggcgactt ccaatagtgc
     3781 agctctaatc gaaggccacc aaggacaccc ccttcttacc ctgacctggt acactatccg
     3841 tacaccgtcg acgagtgccg atgaatcttc gctctgcgca gcggcgctaa tctgttgata
     3901 ggattggtgc acccggcatg attcgaacat gcgaccccct gattcgtagt caggtactct
     3961 atccaactga gctacgggtg cgtggttagg attttgaata taagaaatcc aatgtccaat
     4021 gtccaatgga gggatgtcca atgtccaagg tccaaagtcc aaagtcagtc ggatagcgat
     4081 tcgcttactg cgcaactggc gcaagcggac tttgggcgtt ggagttggga ctttggacat
     4141 ccgaaaccgg acattggaca ttggacttgg gacttgagtg ttagcgcgcg aagtatcccg
     4201 gccgcgtgcg ggcgatcagt tccggatgca caacctcaac tctgatctca tgccacttgc
     4261 cgtcgcgggg acgatcgcca ggtggttgat aggcgaccgt atagagcgtc cgcagatttg
     4321 ccgctacctc tgcgtacaaa cgcgccatct gatccagccg gttaatctca gtcagacgtc
     4381 cccccgtgcg attggcaata ctctccagac gagtgcgtgc cgctgcgtag atccccgtga
     4441 tgtccggacc aggaatcggc aatcgtttcg gatcccctga aggtaaggcg agcggaaaga
     4501 ttgtcactcc ctgacgatca gctaagttca gaacggcatt caaggaagcg ctcgcattcg
     4561 gtttgatggc attgagcgct tcttcgtcgg tttgcgacct ggcaactgcc gcgcgatcga
     4621 catttcgcat ctgcgtatcg agcccatcag ttaagacaac tatcgccttg cgccgtttct
     4681 cctctttctg caattccttc aacgcgtagg tcagcgcttc gtacagatga gtttccccgg
     4741 caccttccgc aatctggata gcgtaggcag tgtctttcct actggcgtca aaattcgcca
     4801 gccgtttgac cttggcattg aactgcacga cagccacacg atcttccgga gccagggcat
     4861 ctagaaaacg agtggtggct agtttcaact gttcgcgaaa gctgagggtc gaacccgaca
     4921 tatcaagcaa cagcaccacg ctgaacggtg cgttggtggg ttcgaattga aagatcgtct
     4981 gtttcactcc gtcctcgtaa atagaaaaat cgttgcgcga gagggaagtg attgcgcggc
     5041 cgcgactatc aactacgccg acgttcagtt gcaccagcga cgttgacgtg cgcacaacgt
     5101 cgtccggttc ctgcgcctgc gagatgctga agctcccgca aacaaacgcg aggccaaaaa
     5161 cgagcaaaag gcggcgaata gagttttgat aaagtttcgc tgagatcatt ggaaaattgc
     5221 tttgtggatt agtagcggga gtatgccttt ccgataaagt ccgtgcaaac ccgtgcgggg
     5281 tagattcggc tgcagacttc ggacgcctga aagtgccttt ccgttgcttc accgctcccg
     5341 agagggggaa tataatgccc ccacgtaatt gagctcaacc tcactgacat ttcggagtcc
     5401 tcaccaatgt atcgaccaaa tttctgttct gaatgcggta ccaagatcgt gcggctgcac
     5461 tggcatccgt ggacgagccg caggttttgc gatggttgtg cgcggcgttt aaggaaagag
     5521 cgactacggc ctccgctgct ggccgcctgc ttgctgctga gtatcggatt tgcaactgga
     5581 cgcgcgttgc ggccgaagcc acccccgctg attattcagc gcagcgcaag ctcaccgcta
     5641 gcgaagagtg aaagccctgg gttgcacggg cagtccggag tgaatgcaaa ctcacagacg
     5701 ccgcccgtgg aagaagtggt ttacatttgc ggcgcgcgca caaagaaggg cacaccatgt
     5761 tcccggcgcg tgcatcagcc ggggcgttgt tggcaacaca agggccggcc ggcgatgttg
     5821 ccggaagaaa agttgattgt taaagattga tgctgttcga tgcttgaatc atggcaagct
     5881 tggtttggga aagccgcgtc tggaatcgtt tgccgagtta ggatcgagtg ttacgataac
     5941 tcttaagtgg aggtcgaaac tatgcagtcg ctggatgcgg tgccacaagt tagcttctca
     6001 ccactgttgc atcgctacac gctcgaggag ttttacgctc ttccagatcc cgatgatcgt
     6061 tctcactacg agttaa
//
LOCUS       DASUEX010000453         2332 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1214062, whole genome shotgun sequence.
ACCESSION   DASUEX010000453 DASUEX010000000
VERSION     DASUEX010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2332
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1214062"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(320..1828)
                     /locus_tag="VFV61_07675"
     CDS             complement(320..1828)
                     /locus_tag="VFV61_07675"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4900396.1"
                     /translation="MIELRNITKRFGNVLANDRVNLTVKPGTIHAIVGENGAGKSTAM
                     RIAYGFYKADAGEILVDGKVREIASPHDAIALGIGMVHQHFMLVDTMTVAENIVLGAE
                     PGSATALDLEKARSEIRRVSEEFKLAVNPDAVIENLSVGQQQRVELLKALYRHARLLI
                     LDEPTAVLTPQEVDEFFAILRRMREQGKTVVIITHKLSEVLAISDEVTVMRDGKVVGS
                     LQTKDTDAAELARLMVGREVLLRVEKPEAKVGEARLSVQNLTITKAGGNKRVDNISFD
                     VSAGEILGIAGVEGNGQTELIEALAGLVNPAHLSGSILFAGRDISREGARLRRELGIA
                     HIPEDRHRRGLLLEFDLAENSILGVHYRKPMVSLGGVFLDGGAVQQRAQEIIEGFDVR
                     PADAELPARALSGGNQQKLIIGREFKVAPKLLLVSQPTRGVDIGAIEFIHRKLVDLRD
                     SGCAVLLVSAELEEVTSLSDRLLIIHEGRIAGEVDPKVATLEEIGLLMTRGH"
BASE COUNT          533 a          663 c          571 g          565 t
ORIGIN      
        1 acgggccacg ataacgcgct gccgaatagc agcccatacg cgtgcaatgg atcatcacca
       61 actagcagga taaggatgcc acctacaata aacgctgcca gcacggcgat cagcggaaag
      121 agcagttctt ttagaagagt catcgaattg ttatcggtgt atgtcctgct ttttgaactt
      181 agtactttgt gcttagttct ttgttcgcga ttcgacccca tcttgcatgc ttaacgaagg
      241 ctttaagccg catcaaacaa acaaagaact aagtacaaag aactaagaac aaagctggaa
      301 atggccgccg ggccatttct cagtgccccc gcgtcatcaa caatccaatc tcttcaagtg
      361 tggcaacttt cggatccact tcaccggcga tgcggccttc atggatgatt agcaagcgat
      421 cggagagcga cgttacttct tcaagctccg cggaaactaa caggaccgca caaccgctgt
      481 cgcgcaggtc cacgagtttg cgatgaatga attcaatcgc gccgatgtcc acacctcgag
      541 ttggctgaga caccaaaagc agtttgggag cgaccttaaa ctctctgcca atgatcaact
      601 tttgctggtt gccgccggat agagcccgcg cgggaagctc cgcgtcggca ggacgcacat
      661 caaaaccctc aatgatttcc tgggcacgtt gttgcacggc gccgccgtca agaaagactc
      721 cgcccagact gaccattggt ttacggtagt gcacgccgag aatggagttt tcagccagat
      781 caaattccag cagcaatccg cgccggtggc gatcctcggg aatgtgcgcg attcccaact
      841 cccgtcgcaa acgcgcaccc tcgcgggaaa tgtctcgtcc ggcaaagagg atactgccgg
      901 aaagatgggc cggattcacc agaccggcga gggcttcaat caactcggtc tgtccatttc
      961 cctccacgcc ggctattccc aaaatctcgc ccgcgcttac gtcaaacgaa atattatcga
     1021 cgcgcttatt gcccccggct ttagtaatcg tcaagttctg gacgctcaaa cgcgcttcgc
     1081 cgacttttgc ctccggcttc tcaacgcgca acaggacctc tcggcccacc atcagtcgcg
     1141 ctagttctgc ggcgtcggtg tccttcgttt gcaggctacc gaccactttg ccgtcacgca
     1201 ttacggtcac ttcgtccgag attgccagca cctccgacag cttgtgagta atgatcacca
     1261 cggtctttcc ctgctcgcgc atgcggcgca ggatggcgaa aaactcgtcc acctcctggg
     1321 gcgtcaggac cgcggtaggt tcgtccagaa taagcaggcg tgcgtggcga taaagagcct
     1381 tcagaagctc aacccgctgt tgttgtccta cggaaaggtt ttcgataact gcgtcgggat
     1441 taacggcgag tttgaactct tcggagacgc gtctgatttc ggatcgcgct ttttcgaggt
     1501 cgagggcagt cgcgcttccc ggctcggctc cgagaacgat attctcggcc acggtcatcg
     1561 tgtcaaccag cataaagtgc tggtgcacca tgccgatgcc tagcgcgatg gcgtcgtgag
     1621 gtgaagcaat ctctctgact ttaccgtcaa ccagaatctc gcctgcatca gctttgtaaa
     1681 atccgtaggc gatacgcatc gccgtagact tacccgcgcc attctcaccg acgatggcgt
     1741 gaatggtccc cggcttcaca gtcaggttga cacggtcgtt tgctagtacg ttgccaaacc
     1801 tttttgtgat gtttcttaat tcaatcatcg tcggttgtca gttgttagtt gttagttcgt
     1861 gttgccctgt gttctgtgac accggttgaa tgccggccaa tcgatttcaa gtatgcattg
     1921 attgtaggcc ccagctcgtc gacaagtggc ttcagttgat cgacttgagc tactgtaagc
     1981 aattgtcgtt tgtaggctcg gcgcaaccaa tactgcgcct cgtgaaaaga gcctcgactg
     2041 atcctaacga agcgcttgtt gtccaaaaag ctaccccgcg cacttccttc cgaaatattc
     2101 gcgccgatgc tatcgacggc tcttaccaat tgtttaccga tagtgtcacg agcaaaataa
     2161 ttccaaccga gtactatgat ccaaactcgg tctgctaatt cctccgccaa ccgataaaca
     2221 cgtaaactct caacgtttga tcttgccatt cgttctccac tggtgaggta tggagataag
     2281 ttttgcagcg atgtctctac tactgactac tgattactga ctacttattc at
//
LOCUS       DASUEX010000454         2294 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1329234, whole genome shotgun sequence.
ACCESSION   DASUEX010000454 DASUEX010000000
VERSION     DASUEX010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1329234"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2240
                     /locus_tag="VFV61_07680"
     CDS             <1..2240
                     /locus_tag="VFV61_07680"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HEX4900397.1"
                     /translation="RLRSEIGRAGVAAEHQRYASHFLEMRAWEPAVRHLVAAEQFDRA
                     ATVIGEHGSEWLASGALSSLAGLADVLPQTALEAHPRSLAMRAEVSRLRGEYEAAQSM
                     FRRAATLLQQQQDAEGEAETLHSLATLARRQGDYPLAFTYLDRAIELSDANSVVRTKC
                     GNTRGLCLVAMGDWIGAEREFRSALQSAEERNDERYSRMIAHNLGTPAGIRGDFGEAL
                     RWLRRMLRSDQKAPMPQEAVAYLNMARCYLYQGDFAATELNLDKALDRCQLFNLVAAR
                     AETFETYGNLFRERSDFERANEYYERAARAYDDAGISLGRTEWYEERALLNLQVGDLG
                     AARSLIDRLLSGRSREKDEIAFQTASLTRARIRTAQGEYESARDELTEALKYFRDHTL
                     YYYEAQACMACATCNLKLHDERQAREQVRRAIDLAVRYDYEYWLEREVLKNPELFAIE
                     ETAELLPADLREQLTQVQKPLSAISVTEIALTSKPVTDLTIRMLGPIEIVRDPARPLA
                     ADAWTTRRSRDILCFIISRRHHRASKDKIIDTFWGETDLEVVEKNFHPTVSHIRKALN
                     SNQSLKQNFLLYRDGDYQLNPEFSYQIDIEEFDRLMGEGEKARRARNFDDCISCYEAA
                     IALYRGEFMQGSYEPWVEEQRTYYREQYLRLLEALAGVAQKEEDWPKATQLAQRILHE
                     DQFREDIHCLIMRGHAAMGNRAAVREHYQQLKRLLQAELGVEPAPETNKLFQELVGGE
                     NLASE"
BASE COUNT          539 a          616 c          674 g          465 t
ORIGIN      
        1 gccggctgcg cagcgaaatt ggacgtgccg gagttgctgc cgagcaccaa cgttatgcca
       61 gtcattttct ggagatgcga gcgtgggagc cggccgttcg acatctggtg gctgcggagc
      121 agtttgaccg cgcggcgaca gtgattggcg aacacggcag cgagtggctt gcctccggcg
      181 ccttaagttc gcttgctggg ctagccgatg ttttgcccca gacggccctc gaagcacacc
      241 cgagatcctt agctatgcgt gctgaagttt cacgactccg aggtgaatat gaggctgctc
      301 agtcgatgtt tcggcgtgcc gcgacgctac tgcaacaaca gcaggatgca gaaggcgagg
      361 cagaaactct ccattctctc gccaccctgg cccgccgcca gggcgactac ccgttagcat
      421 tcacctatct ggatcgggca atcgaactat ccgatgctaa ctcggtagtg cgaacgaaat
      481 gcggaaatac tcgcggcctg tgtctcgtcg cgatgggcga ttggatcggc gccgaacgag
      541 aatttcgaag cgccttacag tctgcagagg aacgcaacga cgagcgttac agtcggatga
      601 ttgctcacaa tctgggcacg ccggctggca ttcgcggcga ctttggcgag gcattgcgtt
      661 ggttgcgccg catgttgcgc agcgaccaaa aggcgccaat gccgcaggaa gccgtggcat
      721 atctcaacat ggcgcgctgt tatttgtatc agggtgattt tgcagccacc gaattgaacc
      781 tcgataaagc actcgatcgt tgccagcttt tcaatctcgt tgccgcgcgc gccgagacct
      841 tcgagactta cggcaatctt tttcgcgaac gaagcgactt tgagagggcg aacgagtatt
      901 acgaacgtgc ggcacgtgct tatgacgacg ccggaataag tctcgggcgt accgagtggt
      961 atgaagagcg ggccttgctc aatttgcagg taggcgactt gggcgccgcg cgcagcctta
     1021 tcgatcgact gctgagcgga cgttcgcggg aaaaagatga aatcgccttt caaaccgcgt
     1081 cgcttacccg ggcgcgcatc cgcactgccc aaggcgaata cgagagcgcc cgcgatgagc
     1141 tgacggaagc gctaaagtac tttcgcgacc acaccctcta ctactacgag gcgcaggcct
     1201 gcatggcatg tgcaacctgt aatctgaaac tgcacgacga gcgccaggcg cgcgaacaag
     1261 tgagacgcgc catcgacctc gcggtgagat acgattacga atactggctg gagcgggaag
     1321 tcctaaagaa tcctgaactc ttcgcgatag aagaaactgc cgagttgttg cccgcggatc
     1381 tacgggagca actgacgcaa gtccaaaaac cgctgagcgc catttctgta accgagattg
     1441 ctctgacgag caagcccgtt actgatctca caatcagaat gctcgggcca atagagatcg
     1501 taagagatcc agcgcgccca ctcgctgccg atgcctggac cacgcggcgt tcgcgggaca
     1561 tcctctgctt cattatttca cggcgtcatc accgcgcttc aaaagacaaa atcatcgaca
     1621 cgttctgggg cgaaaccgat ttggaggtgg tggagaagaa ttttcatcct acggtgtcgc
     1681 acattaggaa ggcgctcaac agcaatcagt ccttgaagca gaacttcctc ctttaccgcg
     1741 acggcgatta tcagctcaat ccggagtttt cctatcaaat cgacatagag gaattcgacc
     1801 gcttgatggg cgaaggggaa aaagcccggc gagctcgcaa ctttgacgat tgtattagct
     1861 gctatgaggc ggcgattgcg ctttatcgtg gcgaattcat gcaaggaagc tatgaaccgt
     1921 gggttgagga gcagagaact tattatcgtg agcagtatct gcgactactg gaggcgctgg
     1981 cgggcgttgc tcagaaagaa gaagactggc ccaaggcaac gcaacttgcc caacgcatcc
     2041 tgcacgagga ccagttccgg gaggatattc actgcctgat catgcgcggc cacgcggcta
     2101 tgggtaatcg cgcggcggtt agggagcatt accaacaact gaaaagactc ttgcaggcgg
     2161 agttaggtgt ggaaccggcg ccggagacta acaagctttt ccaggaatta gtcgggggtg
     2221 agaatctcgc gtcggaataa tgaaaaaatc gcaatctggg cgcaacaact tcagcaaggc
     2281 ggctctttaa gatg
//
LOCUS       DASUEX010000455         2235 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1214391, whole genome shotgun sequence.
ACCESSION   DASUEX010000455 DASUEX010000000
VERSION     DASUEX010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1214391"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="VFV61_07685"
     CDS             complement(<1..513)
                     /locus_tag="VFV61_07685"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900398.1"
                     /translation="MEFAEALRYLLSLGHETLAIKLGLKNISLLLAKLGNPQQSFRSV
                     QIAGTNGKGSTAFMLESICRAAEIKTGLFTSPHLISITERIRIDGMELTPESFAKYAT
                     RVRAAAETLLLDSEIEALPSFFEQVTAIAMIAFDEAGIELAILETGLGGRLDATTAVG
                     AEIVGITPISM"
     gene            complement(538..1455)
                     /gene="accD"
                     /locus_tag="VFV61_07690"
     CDS             complement(538..1455)
                     /gene="accD"
                     /locus_tag="VFV61_07690"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HEX4900399.1"
                     /translation="MTRMPWFSKKSKRIEAVPPEERVVKTENVFVKCDSCEAHLYKRE
                     LEESLQVCAHCGHHFRIGARERLAMLFDDGNYEELDAEVISIDPLEFVDSKPYPTRLH
                     HAKKTSGLPEAVINGRGKVGDHLVLAAAMDMSFIGGSMGSAVGEKVTRLIERAIKESG
                     AVVVFSASGGARMQEGTLSLMQMAKISAALAMLDEARLPFISVLTDPTTGGVTASFAM
                     LGDVIIAEPKALIGFAGPRVIEQTIRQRLPKDFQKSEFLLEHGMLDAIVDRREIREYI
                     IKLLSFMMNPEIHSESLPRPANARAVSRK"
     gene            complement(1553..2131)
                     /gene="cobO"
                     /locus_tag="VFV61_07695"
     CDS             complement(1553..2131)
                     /gene="cobO"
                     /locus_tag="VFV61_07695"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF004637.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEX4900400.1"
                     /translation="MDQKEKKYKWRREDYQEKTRGLLMVFTGNGKGKTTCALGLMMRA
                     AGRGLKCCMIQFMKSRHDRYGEHESAEKLGIEVHTMGDGFTWDTKNPEQDRATARATW
                     ELCLQKLRSGEYDLLVFDELVYVLSYQMLPLEEVLATFKAIRAEQPALHIVVTGRDAP
                     QDLIDAADLVTEMREIKHPFNVGIRAQQGIEF"
BASE COUNT          498 a          602 c          600 g          535 t
ORIGIN      
        1 catggagatt ggtgtaatgc cgacgatctc ggcgccgact gcggtggttg cgtcgagcct
       61 gccacccaga ccagtctcca ggattgccaa ctcaatccca gcttcatcaa aagcgatcat
      121 tgcgatagcg gtaacctgtt caaagaatga tggcagcgcc tcaatctctg aatctagtaa
      181 caatgtttct gctgcagcgc ggactcgggt cgcatacttc gcaaacgact caggcgttag
      241 ctccatcccg tcaatgcgaa tacgttctgt gatcgaaatc aggtgtgggg aagtaaagag
      301 tccggtcttg atctcggcag cgcggcaaat tgattccaac atgaaggccg tcgaaccttt
      361 accattggtg ccggcaattt ggaccgagcg aaatgactgc tggggatttc cgagctttgc
      421 gaggaggagt gaaatgtttt tgaggcccag cttgatagcc agtgtctcat gcccgagact
      481 cagcaggtaa cgaagcgcct cggcaaattc catcttgtca gtccgaagaa ttagagatta
      541 cttgcgtgag acggcccgag cgtttgcggg acgtggcaag ctctcgctgt ggatctctgg
      601 attcatcatg aagctaagca gtttgatgat gtattcgcga atctcacgcc gatcaacgat
      661 ggcatcaagc atgccgtgct cgagtaaaaa ctctgatttc tgaaagtcct tagggagtct
      721 ttgacggatt gtttgttcga ttactcgcgg gccagcaaag ccaatcagcg cttttggttc
      781 agcgataata acatcaccaa gcatggcaaa actggctgtg acgcccccag tggttggatc
      841 cgtcagcacc gaaataaagg gaaggcgagc ctcatcaagc atagccagcg cagcagaaat
      901 ctttgccatc tgcattagcg agagcgtccc ttcctgcatc cgagcaccac ccgatgcgga
      961 aaatacgacc acggcgccac tctctttgat tgctcgctca atcagacggg taaccttttc
     1021 acccacggct gagcccattg agccgccgat gaatgacatg tccatggccg cggctagaac
     1081 cagatggtcg cctactttgc cgcgtccgtt gattactgcc tccggtagtc ccgaagtctt
     1141 cttcgcatga tgtaaacgag tcggatacgg tttgctgtcg acaaattcga gcgggtcaat
     1201 cgagatcact tcagcgtcga gttcttcgta gttgccgtca tcaaagagca tggccagccg
     1261 ctcgcgggca ccgatgcgaa agtgatggcc gcaatgggca cacacttgga gcgactcctc
     1321 aagttcacgt ttgtagaggt gggcctcgca ggagtcacac ttgacgaaga cattctcagt
     1381 tttgacgaca cgctcttcag gtggaaccgc ttcaatacgc ttacttttct tgctaaacca
     1441 gggcatgcgc gtcatccagg gattaatgtc cttaggggat tacagtgaac ggctcaggag
     1501 attagcacat tggggggaaa cgatgaaggc ggctttcgcc caatgttttg gactagaact
     1561 cgatcccctg ttgtgcccga attcccacat tgaagggatg tttgatctcg cgcatttccg
     1621 tcaccagatc cgcggcgtcg atcaggtcct gcggggcatc gcggccggtg accacgatat
     1681 gcagtgcggg ttgttcagcg cggatggcct tgaaagttgc cagcacctct tcaagcggca
     1741 acatctggta gctcaaaacg taaaccagtt catcaaacac gagcagatcg tattcgccgc
     1801 tacgcaactt ctgaaggcag agttcccacg tggcccgtgc agtggcgcga tcctgttcag
     1861 ggtttttcgt gtcccaggta aagccatcac ccatcgtgtg cacttcaatt ccgagcttct
     1921 cagctgattc atgctcaccg tagcgatcgt ggcgcgattt catgaactga atcatgcaac
     1981 acttcaaacc tcgtccggct gcacgcatca ttagaccaag ggcgcaggtt gtcttgcctt
     2041 tgccgttacc ggtgaagacc atcagcaggc cgcgagtctt ttcctggtag tcctcgcgac
     2101 gccacttgta tttcttctct ttctggtcca tgggagtttc gtgccgaggg gcgaggaatc
     2161 agtcaggctc ggtcacgtcg atttcggcgg cgagcttgag cgcgtcatcc gttatgtctc
     2221 tcgatccgtt ggcgc
//
LOCUS       DASUEX010000456         5674 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_645630, whole genome shotgun sequence.
ACCESSION   DASUEX010000456 DASUEX010000000
VERSION     DASUEX010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5674
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_645630"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..799)
                     /locus_tag="VFV61_07700"
     CDS             complement(<1..799)
                     /locus_tag="VFV61_07700"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX4900401.1"
                     /translation="MWLYYLFAAIIIWLGIISLRGGFRFASYVNSQLAQPLADFTPFA
                     TVIVHCRGREPGLAENLAALFEQCYPSYEIVFVTDSESDPSLEVIQNIVLAKASAIAA
                     RVLIAGPATESGQKVHNLRAAVGQLDPRSEVLVFVDSDARPGRNWLRSLVAPLVDHEL
                     GAATGYRWFVPVNGGLASRVRSSWNASIASALGADRKKNFCWGGSTAIRRETFVRLNI
                     RERWQGSVSDDFTMTRVLGEANLPIHFVPACLVASPGDCDLGELLEFS"
     gene            complement(1162..2769)
                     /locus_tag="VFV61_07705"
     CDS             complement(1162..2769)
                     /locus_tag="VFV61_07705"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HEX4900402.1"
                     /translation="MSIVISTISMFLIQNLFSIHHLLLQQANTTALFNIADLQLIAPE
                     LILTVCACTALVLEVILPYRKSKAIAYFVLVSIGIASASLAIQWWLIRASLPLTGFYG
                     MVRIDGFALLFKAIFLIAAALAVAISTRYLDIEGEQHGEYYALVLFATVGMLFLACGY
                     DLIVLYISLELMALTFYVLVAFTKREKRSNEAAMKYFLLGAFSSGVLLYGMSLLYGIT
                     GSTNLGEIGTALAQIVPAMSDTTEAAESLINLRPMLLLAMIALAAGLFFKIAAVPFHM
                     WAPDAYEGAPTSVTAFLSTGSKAASFALYARIFLEALPAMRVDWAPLLGLVAAITILV
                     GNWAAVTQENSKRLLAYSSISNAGYLLLGLVAGNSYGYIGLLIYLLVYTLMNMGAFGI
                     IISLRRRGIIGDNVEDFTGLALKSPGMAAMMAIFMLSLGGLPMTGGFIGKYFLFGGLL
                     QRGQADGKNWYYWLAIWAIINTVVSFYYYIRFIKVMYLGDRIADDKPLELSPVLRTAL
                     VASLVGIIFIGLYPQPFISIAQRLLTP"
     gene            complement(2811..4496)
                     /locus_tag="VFV61_07710"
     CDS             complement(2811..4496)
                     /locus_tag="VFV61_07710"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEX4900403.1"
                     /translation="MKTYILSIVTWLPTVGAIIILALFKKNQGSLIKKFATAWFGLAF
                     LASLLLMAYDRSRGGMQFLEDYQWIPVIGARYQMGADGVAVLLIVLTTLLGVIAALSS
                     WKYIEKREKEYYVLLLLLQTAVVGVFASMDLFLFYLFFEVSLVPMYFLIGIWGGENRL
                     YAAIKFFLYTLVGSVVMLLGVLKMYFLTQDTALTSQITQTTLDLVAGNSQAAALVSST
                     IQTATANGTGTFNMLYMQALGSVMPMGTMQVLLFFAFALGFAIKVPMFPFHTWLPDAH
                     VEAPTAGSVILAGILLKLGTYGFFRFNLPMFPAASADESYFGSFGVRSVMIFLAIIGI
                     IYGALAAMYFVVKKDGDVKKLVAYSSVSSMGIVMLGLFALNPNGVNGAVLHMINHGIY
                     SAALFLLVGIIYERRHTRIVAEFGGLSHVMPGYAAVFLSMVMTAIGLPLLCVFISEFL
                     SLRGAFEANPWWAAWGALGIILNAGYMLWLYQRMFFGPIDNPKNEKLSDLSGREWLYM
                     TPLVIMSLWIGVYPQTFIDYIRQPVNAVVKHVRPDYPVPPRPGELPTQHRAEK"
     gene            complement(4685..>5674)
                     /locus_tag="VFV61_07715"
     CDS             complement(4685..>5674)
                     /locus_tag="VFV61_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900404.1"
                     /translation="AFYMTRLVAMTFWGKFRGTDSQHQGHADASHGHGTHTPHESPAS
                     MWVPLVALAVFATIGGFIGVGPAFKFLSGSEHPGGRLNIVNYLDPIIWNSATGEFGKA
                     HASEAGEHGAEPIASAPAGETETTEHTAVANESQTATHAATTSEGLNLAHAAEGALGS
                     HLASEWLFIVISLVVAAIGIALGWLFYVKSPHLPDAWARRLKPLYEASYNKYWVDEFY
                     GWAVTRRVMDAARAVFAVDSKVVDGAVNGSAWLTRLTSTITGATDKYFVDGIVNAIAA
                     FIMRLMSPLLRAAQTGLTQNYALVMVFGLVVAVALFFAGDIVGLVRSFVALGI"
BASE COUNT         1464 a         1545 c         1583 g         1082 t
ORIGIN      
        1 tggagaattc cagcaactct cccaggtcgc aatcccccgg tgaggccacc agacacgcgg
       61 gaacaaaatg aatcggcagg ttagcctctc cgagaacgcg agtcatggtg aaatcgtcag
      121 acacacttcc ctgccaacgc tctcttatgt tcaatcgcac aaaagtctca cgccgaatcg
      181 ccgtagaacc accccaacaa aagtttttct tccgatctgc acctaatgcc gaggcaatcg
      241 aagcgttcca tgacgaacgc acacgtgagg cgagaccgcc gttcaccgga acgaaccaac
      301 gatagccggt ggcggcaccg agttcgtgat caaccagcgg ggctaccagc gaccgcagcc
      361 agtttctgcc ggggcgtgca tccgaatcga caaataccaa cacctcagag cgcggatcga
      421 gttggcctac tgcggcgcgc aggttgtgca ccttttgacc gctctcagtg gcggggccag
      481 cgattaagac acgagccgcg atggcggacg ccttcgccag gacgatgttt tggattactt
      541 ccagtgaagg gtcgctctcg ctgtcagtaa cgaagactat ttcgtaactg gggtagcact
      601 gttcgaaaag tgcggccagg ttctcagcaa gaccgggctc gcgaccgcga caatgaacga
      661 tcacggtagc gaaaggggtg aagtcggcga gcggttgcgc gagttgggag tttacgtaag
      721 atgcaaaccg gaagccgccg cgtagtgaaa tgatgcctaa ccaaattatg atcgcggcga
      781 agaggtaata gagccacata cacggttcga gccggcgaaa ctattcccct ctcatcgccg
      841 cgagagttat caaccgcagt agcgcaaaca tgttcgggac ctgatccggc acgaacgtaa
      901 caatgctggt ctgccgtgcg cagcaagcac acaacgccgg tggcggcgga cacacctgct
      961 ccggcaggcc cgagaacttg tcgaagtatt ctcttgctgc gccatcgtcg ttgacgaatt
     1021 tgcattctga gcactaacca gtggagtgat aaacacgagt tgggtagaga gtgtcagaac
     1081 agcgaacagg attagtaagc gaacctgagg tgtcgtcatt tgagttctcc cgcgtaggtc
     1141 gaattggtca ggggaaactg ctcaaggtgt taagagtcgt tgggcaatgc taatgaacgg
     1201 ctgtggataa aggccgataa agatgatgcc cacgagtgag gccaccagcg cggtacgcaa
     1261 aactggcgaa agctccaacg gcttatcgtc cgcgatgcgg tcaccgaggt acatcacttt
     1321 gataaagcgg atgtagtaat agaaagaaac cacggtgtta ataatcgccc aaatggccag
     1381 ccagtagtac cagttcttcc cgtcggcttg cccgcgctgc agcaaaccac cgaaaagaaa
     1441 atatttgccg ataaaaccac ccgtcatcgg cagtccgccc agcgaaagca tgaagatggc
     1501 catcatggcc gccatgcccg gagacttaag ggcgaggcca gtaaaatcct cgacgttatc
     1561 gccaatgatg ccgcgtcgac gcaggctgat gatgatgccg aacgcaccca tgttcatgag
     1621 cgtgtaaacc agaagataaa tcagcagtcc gatgtagccg taggaattgc cggccaccag
     1681 tcccagtagt aagtaaccgg cgttggagat ggatgaatat gccagcaggc gtttagagtt
     1741 ttcctgggtc acggcggccc agttccccac caggatggtg atggccgcta ctaagccaag
     1801 caaaggcgcc cagtcaacgc gcatcgccgg caaggcttcg agaaaaatgc gcgcatacag
     1861 cgcgaagctg gcagccttag agccggttga gagaaatgcg gtgacggaag tgggcgcgcc
     1921 ttcataagcg tcgggtgccc acatgtgaaa tggcactgcc gcaattttga agaacaagcc
     1981 tgcagcgagc gcgatcattg ccaacaacaa catcggccgc agatttatga gactctcggc
     2041 agcctccgtg gtatcactca tggcgggcac aatctgcgcc agggcagtac cgatctcgcc
     2101 gaggttcgtt gacccagtaa ttccgtagag cagcgacatc ccataaagca gcacgcctga
     2161 agagaacgcc cccagcagga aatacttcat cgcggcttca ttcgatcgct tctcgcgctt
     2221 ggtaaaagcg acgaggacgt aaaacgtcaa agccatcagt tccagactaa tgtacagaac
     2281 gatcaagtcg tagccacacg ccagaaacag catgccgacc gtcgcaaaga gcaccagcgc
     2341 atagtactcg ccatgctgtt cgccctcgat atcgagataa cgggtagaaa tcgccaccgc
     2401 gagcgcggcc gcgatcagaa agatggcttt gaagagcagt gcaaagccat caatgcgcac
     2461 catcccgtag aatccggtta gcggcaaact ggcacggatg agccaccatt ggatcgcgag
     2521 ggacgcgctc gcgatcccaa tgctgacgag gacaaaataa gcgatcgctt tgctcttgcg
     2581 ataaggaaga atcacttcca gtacgagagc agtgcaggca caaacagtga ggatcaattc
     2641 cggcgcgatt aattgcaggt cggcaatgtt aaagagcgca gtggtgttcg cttgctggag
     2701 aagcaaatga tgaatgctga acaaattctg aataaggaac attgagatag ttgagatcac
     2761 gatagacata ttaaggagcg gaattcatcg ttcatcattc atcatgaaga tcatttctca
     2821 gctcgatgtt gcgtggggag ctcgccgggc cgcggaggca cgggataatc cggacgtacg
     2881 tgtttgacta ccgcattgac cggctggcga atgtaatcaa tgaaagtctg cggatagacg
     2941 ccgatccaca gcgacatgat tacaagcggc gtcatgtaga gccattcccg cccgcttaag
     3001 tcggaaagtt tttcgttctt gggattatcg atcgggccga agaacattcg ctggtacagc
     3061 cagagcatgt aaccggcatt caggatgatg cccagggccc cccatgctgc ccaccaggga
     3121 ttcgcttcaa acgccccccg cagcgaaaga aactcggaaa tgaaaacgca gagcaagggc
     3181 aagccaatcg ccgtcatcac cattgacaga aagactgccg cgtaccccgg catcacatgc
     3241 gaaagcccgc caaactcggc gacgattctc gtgtgccgtc gttcataaat aattcccacg
     3301 agcagaaaga gggcggccga gtagatgcca tgattgatca tgtgcaggac ggcgccatta
     3361 acaccattgg gattgagggc aaacagcccg agcatgacaa ttcccattga agagactgac
     3421 gagtaagcca cgagtttctt tacgtcaccg tccttcttca cgacgaagta catcgccgca
     3481 agggcgccat agatgatgcc gatgattgcc agaaagatca ttacgctacg cacaccgaag
     3541 gaaccgaagt agctttcatc agccgaggca gcgggaaaca tcggtaggtt gaagcggaag
     3601 aagccgtagg tacccagctt cagcaagatg ccggcaagga tcaccgagcc cgccgtaggc
     3661 gcttcaacgt gcgcatcggg cagccaggtg tgaaacggga acatcggaac cttgatcgca
     3721 aagccaagcg cgaaggcgaa gaaaagcagg acttgcatcg tgcccatcgg catcaccgag
     3781 ccgagtgcct gcatgtagag catgttgaag gtgccggtgc cgttcgcagt tgcagtttgg
     3841 attgtgctcg aaacaagtgc agccgcctgg ctgtttcccg ccaccaaatc gagtgtggtc
     3901 tgggttatct gcgatgtgag cgcagtgtcc tgggttagga aatacatttt gaggacaccg
     3961 agtagcatca cgaccgaacc cacgagtgta tagaggaaga atttgattgc tgcgtaaagc
     4021 cggttctcgc caccccagat accgatcagg aaatacatgg gcacgaggga gacttcaaaa
     4081 aacaggtaga agaggaaaag gtccatcgat gcaaagacgc cgactacggc cgtctgcagg
     4141 agcagtagca gaacgtagta ttccttttcc cgcttctcaa tgtatttcca tgaagacaaa
     4201 gctgcgatca cgcccaggag agtcgtgagc acgataagca acacggccac gccgtcagcg
     4261 cccatctggt agcgtgctcc gattacggga atccactgat agtcctcgag gaactgcatg
     4321 ccgccgcgcg accggtcata agccatcagg agcaaggagg ccaaaaaggc gagcccaaac
     4381 caggcggtgg cgaatttctt gatgaggctc ccctggtttt tcttgaagag ggcaaggatg
     4441 atgatggcgc caacggttgg aagccaggtg acgatggata gaatgtaggt cttcataatt
     4501 ggcggttgtc agtagtcagt tgtcagtagt ccgtagtccg tggaagaagc gccttttagt
     4561 gtttattcgt atcattttgt ggatcgaatc ctagccgtct gtcctgcata cgatccacga
     4621 aatcacacca accaacaccg agcaggcgtc actgaccact aactactgac cactgacaaa
     4681 tcgtctagat ccccaacgcg acaaaacttc tcaccaaacc cactatgtcg ccggcaaaga
     4741 aaagtgcgac ggcaaccacc aacccaaaca ccatcacaag cgcgtagttt tgcgttaagc
     4801 cggtttgcgc ggcgcgcagg agcggcgaca tcaggcgcat gatgaaagcg gcgattgcgt
     4861 tgactatgcc atcgacgaag tacttatccg ttgctccagt gatcgtactg gtgagccgag
     4921 tcagccaggc gctgccgttc accgcgccgt caaccacttt cgaatcaacc gcgaaaaccg
     4981 cgcgggctgc gtccatgacg cggcgcgtga ctgcccagcc ataaaattca tcgacccaat
     5041 acttgttgta gctggcttcg tacagcggtt tgagacggcg cgcccaggca tcgggaaggt
     5101 gcgggctctt gacgtagaac aaccagccaa gcgcgatacc gatggccgcg accacgagtg
     5161 agatcacaat gaagagccac tcggaagcca gatgactgcc cagtgcacct tccgcagcat
     5221 gggccaggtt gagtccctcg cttgtcgttg cggcgtgagt cgcagtctgg ctttcgtttg
     5281 cgacggctgt atgttctgtg gtttccgtct ctccggccgg cgccgaggcg ataggttccg
     5341 cgccgtgctc gcccgcctcg ctcgcgtgcg ctttaccaaa ctctccggtg gcagaattcc
     5401 agatgatggg atcaaggtag ttgacgatat ttaggcgacc gcccggatgt tcgctcccgg
     5461 aaaggaactt gaatgccgga ccaacgccaa tgaatccgcc aatcgttgca aataccgcca
     5521 aagcgacgag cggaacccac atagacgccg gcgattcatg aggtgtgtgg gtgccatgcc
     5581 cgtgactcgc atcagcgtgg ccttgatgtt gcgagtcagt gcctctgaac ttcccccaga
     5641 aagtcatggc cactaaccgg gtcatataaa acgc
//
LOCUS       DASUEX010000457         2785 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1665204, whole genome shotgun sequence.
ACCESSION   DASUEX010000457 DASUEX010000000
VERSION     DASUEX010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2785
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1665204"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="VFV61_07720"
     CDS             <1..338
                     /locus_tag="VFV61_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900405.1"
                     /translation="RAQAAPALAGNSDLSGTWLLNVLVGLNTFPGTLILEEQQGALSG
                     VLQSPFGVTKLERGSLDDQGFRFQSRARVEGRIVEMTIEGRVSGAEIRGTVRSEIGFA
                     RFTGTRQKS"
     gene            360..1802
                     /locus_tag="VFV61_07725"
     CDS             360..1802
                     /locus_tag="VFV61_07725"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX4900406.1"
                     /translation="MTRVTMMRRLTIQVLICSLITGISLPQAFAQDRSAPRETLIRNA
                     TVLTVTRGTLTNADVLIRNGKIAAIGKNVRAAGDAKIIDATGKFVLPGIVDCHSHSML
                     DTINEGTLSVTSMARTSDVLNPNDIDLYRALAGGVTSLNLLHGSANAIGGLNTVVKIK
                     FGRPAAEFIFPGAMPGIKFALGENPKRSNSQPQPGQTRRYPMTRMGVEETIRDAFTRA
                     RDYKKSWDEYRASTTRGEKNLVPPRRDLQLDPLVEVLEGKRYVHAHCYRADEILMLIN
                     LANDFGFKIKTFQHTLEGYKVAREIAQHGAGASIFADNWAYKIEAYDAIPYNAAIMTR
                     AGVIVSMNSDSDERARRLNIEAAKAMRYGDLTEEEALKLVTWNPAVQLGIQQRVGSLE
                     VGKDADLAIWNGHPLSVYARVDTTFVDGEIFFDRQEDLARRPELLKERAQLEAAEPNR
                     PPAAGTSPQAPRRRRPTNSDDDMWEGGNQP"
     gene            1799..>2785
                     /locus_tag="VFV61_07730"
     CDS             1799..>2785
                     /locus_tag="VFV61_07730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900407.1"
                     /translation="MKNSSWRDAGMGRRRDAAMRGRVVRLILTMIVTLPGLIAFGQQP
                     LTPTTQSGLMPARGTFVIRNARIVTVSGADIENGTVVIREGKIAAVGTTVDIPANSQT
                     IDARGLSIYPGMIDAGTAMGLVEVGQGAFGTVDTGEVGDLNPHAKAIIAVNPHSAHIA
                     VTRVDGVTSTLSLPTGGLISGQGALLNLVGTTPLEMAVVPQAVLVINYPRTTGGGGGF
                     FAQQPVNLTEALATANRQLEQIRKVLRDAEAYGRAVDAYSKDPKLPRAEQNLILEALV
                     PYVRGERPVVFRASRESEIRGVIKFAEEMKLKAIILGGEDSVKVAGLLKEKNV"
BASE COUNT          643 a          774 c          792 g          576 t
ORIGIN      
        1 gtcgcgcgca ggcggcgccg gcgttagcag gcaattccga tctgtcgggc acctggctgc
       61 tcaatgtgct cgtaggcctt aatacatttc caggaaccct gatccttgaa gagcagcagg
      121 gagctttgag tggtgtgctg caaagtccat tcggagtcac caagctggaa cgtggctccc
      181 ttgatgacca gggttttcgc tttcaatcgc gggcccgcgt cgaaggccgc atcgtggaaa
      241 tgactatcga aggacgcgtc agcggcgcag agattcgcgg cactgtcaga tcagaaatcg
      301 gttttgcccg cttcaccgga acgcggcaaa aatcgtaatc gccaatcagt ccagccttga
      361 tgaccagagt gacaatgatg agacgactaa caatccaagt gctaatctgc tcgctaatca
      421 ccggcatttc tttgccgcag gcgtttgcgc aggatcgcag cgccccacgg gaaactctta
      481 ttcgcaacgc cacggtgctc acggtcacac gcggaacgtt aaccaatgcc gatgttttga
      541 ttcgcaacgg aaagatcgct gccattggta agaacgtacg agccgccggc gacgcgaaga
      601 taattgacgc gaccggaaaa tttgtgttgc ccggcattgt ggactgtcac tctcactcga
      661 tgctggacac gatcaacgaa ggcaccttat ctgttacttc gatggcgcgc acgagcgacg
      721 ttctcaatcc caacgacata gatctctatc gagcgctggc gggtggcgtc acgagtctta
      781 acttgttgca tggttcggcg aatgcgattg gtggcttaaa cacggtagtg aagatcaagt
      841 ttggccgccc ggctgccgaa ttcatctttc ccggagcaat gcccggaatt aagttcgcac
      901 tgggagaaaa tcccaaacgc tcaaactctc aaccgcagcc tggtcaaacg cgccgttatc
      961 ccatgacacg catgggcgtc gaggaaacca tacgcgatgc cttcacacgg gcgcgcgact
     1021 acaaaaaatc ctgggacgag tatcgggcca gtactacccg cggtgaaaag aacctcgttc
     1081 cgccgcgtcg agacttgcaa ctggatcctt tggtcgaggt gctcgaagga aaacgttacg
     1141 tgcacgctca ctgctaccgc gccgacgaaa tcctgatgct gatcaacctc gcgaacgatt
     1201 ttggcttcaa gatcaaaacc ttccagcaca cgcttgaagg ttacaaggtg gctcgcgaga
     1261 tcgcgcaaca cggcgccggt gcttcgatct tcgccgacaa ctgggcctat aagatcgagg
     1321 cgtacgatgc tattccctat aacgctgcga tcatgacgcg cgccggtgtg atcgtttcca
     1381 tgaattcaga ctccgacgag cgcgctcgcc ggctgaacat cgaagcagct aaagcgatgc
     1441 gttacggtga cctcacagaa gaagaagctc tcaaactcgt cacctggaat ccggccgtac
     1501 aacttgggat tcaacaacgt gtcggttcac tggaagttgg taaagatgct gatctggcaa
     1561 tctggaatgg tcatccgctg agcgtctatg cccgcgttga taccacgttc gtcgatggcg
     1621 aaatcttttt cgatcggcaa gaggatctag cgcggcgacc tgagttgctc aaagaacgtg
     1681 ctcaactcga ggctgctgaa cccaatcgtc ctcccgccgc gggaacttca ccgcaagcac
     1741 cacgtcgcag aaggcccacc aattccgacg acgatatgtg ggaaggaggg aaccagccat
     1801 gaagaactcg agttggagag acgcggggat ggggagacgc cgagacgcgg cgatgcgcgg
     1861 acgagtggtg cggctaatct taacaatgat cgtgacgcta ccgggtttga tagctttcgg
     1921 gcagcagccc cttacgccca ccactcaatc tggcctgatg cccgcacgcg gcactttcgt
     1981 gattcgcaat gcgcgcatcg ttacagtgtc cggtgctgac attgaaaacg gcacggtagt
     2041 gatccgtgaa ggaaagatcg cagccgtcgg gaccactgtc gatataccgg ccaactcgca
     2101 gacaattgac gcgcgtggtc tttcaatcta tccgggaatg attgacgcgg gcactgcaat
     2161 ggggctggtc gaagtcggac aaggtgcatt cggcacggta gataccggcg aggtcggcga
     2221 tctcaatccg cacgcaaagg cgatcatcgc ggtaaacccg catagcgcgc acatcgccgt
     2281 cacccgagtc gatggcgtta ccagcactct gtcgcttccc acgggcggct tgatttccgg
     2341 acaaggtgcg ctgctcaacc ttgtgggtac aaccccactc gaaatggcag tcgtgcctca
     2401 ggccgtactt gtaattaact atccccgcac gacaggaggc ggtgggggtt tcttcgctca
     2461 gcaaccagtc aatctcactg aagccctggc cactgccaat cgacaacttg agcagattcg
     2521 caaggtcttg cgcgatgcgg aagcctacgg tcgcgccgtg gatgcgtatt ccaaagatcc
     2581 aaagttgccg cgtgcggaac agaatctgat tttggaagct cttgtgcctt atgtgcgggg
     2641 cgagcgaccg gtggttttcc gcgcctcgcg tgagagcgag attcgcggcg tcatcaagtt
     2701 tgccgaagag atgaaactca aagcgatcat tcttggcggc gaggattccg tgaaggttgc
     2761 cggtttgctg aaggagaaga atgtt
//
LOCUS       DASUEX010000458         2071 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1214981, whole genome shotgun sequence.
ACCESSION   DASUEX010000458 DASUEX010000000
VERSION     DASUEX010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1214981"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="VFV61_07735"
     CDS             <1..344
                     /locus_tag="VFV61_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900408.1"
                     /translation="NRNRLVLKPNDDYGGHGITIGWNSDEISWSKALSEAVAQGDYVV
                     QERVPTAREMFPALKDDGTIEFAEQLVDLDPLLFNGKVGSAFTRLSFTELANVSSGGG
                     MVPTYIISQKT"
     gene            389..724
                     /locus_tag="VFV61_07740"
     CDS             389..724
                     /locus_tag="VFV61_07740"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX4900409.1"
                     /translation="MVDELNEPAPLPDERRKHDRSRLIVDVFFNGQDATGVASTQDIS
                     VGGLYMNTQAHLPEGALLLLRIPLPDGQEVIVNGEVAYVNPGSGVGVSFQGLSAESRT
                     LLESALRRE"
     gene            724..1245
                     /locus_tag="VFV61_07745"
     CDS             724..1245
                     /locus_tag="VFV61_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900410.1"
                     /translation="MSSQEASQIRWRDLALDDREALSGKLVGLYGATRDDLAFADLTE
                     DKQQGLLLLLNRMRAKRVWHLVRRVQNVYGLHGVGLSFSAWPMIESTLTARADFTRRF
                     ANHKDCSGGFYEKGRPRAVLHFLYQNGEPRKWYVHFDLFSPVYSPRSAASHVRHEVLR
                     KFRPDWKTIKQYV"
     gene            1305..>2071
                     /locus_tag="VFV61_07750"
     CDS             1305..>2071
                     /locus_tag="VFV61_07750"
                     /inference="COORDINATES: protein motif:HMM:NF010039.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate-amine ligase"
                     /protein_id="HEX4900411.1"
                     /translation="MFDQFTLGIEEEFQIVDPKSRELRSHVSEFLEEGKMILGEQIKP
                     EMIQSMIEVGTGVCKNIQEARADITRLRSIISGLAGKTGLAIVAASTHPFSHWQDQKI
                     YDDERYSLLVQELQTVARSLLIFGLHVHVGVADLDRRIHIMNAARYFLPHVLALTTSS
                     PFWMGHSTGLKSYRSEVFKQFPRTDIPDHFDSYSSFQRYVDLLVKTGCINDGKKIWWD
                     LRPHPIFPTLEFRICDIPTRVDDTVAIAALFQAIVAKL"
BASE COUNT          460 a          532 c          589 g          490 t
ORIGIN      
        1 caaaccgtaa ccgcctggta ttgaaaccca acgacgacta cggcggccac ggaattacga
       61 ttggttggaa cagcgacgag attagttgga gcaaggcctt gagtgaggct gtcgcccagg
      121 gggattacgt cgttcaggag cgcgtgccta ccgcccgcga aatgtttccc gcgctcaaag
      181 atgatggcac gatagaattt gccgagcagt tggtggatct tgacccgtta ctctttaatg
      241 gcaaggtggg gtcggctttt acgagacttt cgtttactga actcgccaac gtatcttcgg
      301 gcggcgggat ggttccaacc tacattatta gccagaagac ttagtcgcac gatcaaagtg
      361 cagcgcctgg cccacgggag agcctcacat ggttgatgag cttaatgaac ctgcgccgtt
      421 gccggatgag cgcagaaaac atgatcggtc ccgcctgatt gtcgacgtct tcttcaacgg
      481 acaggacgcc acgggtgtgg ccagcacaca ggacatcagc gtgggtgggc tttacatgaa
      541 cactcaggca cacttgcccg agggcgcgct cttgttgttg cgaattccgt tgccggacgg
      601 tcaggaagtg attgttaacg gcgaagtagc ctacgttaat cccggcagcg gcgtaggggt
      661 aagcttccag ggtttaagcg cggaatcgcg cacgctgctg gaaagcgcac tcagacgcga
      721 gtaatgtcca gtcaggaagc ttcccaaatc cgttggcgtg atcttgctct tgatgatcgt
      781 gaagcactgt cgggcaaact ggttgggttg tacggggcaa ctcgtgacga tctggcgttt
      841 gccgatctaa ctgaagataa gcagcaaggg ctgctgctgc tcctgaatcg gatgcgcgcg
      901 aagcgagttt ggcatctggt gcgcagggtg caaaacgtct atgggctgca cggtgtcgga
      961 ctttcgttta gcgcctggcc gatgattgag tcgactctaa cggctcgcgc agacttcacg
     1021 cggcgctttg cgaatcacaa ggattgcagt ggcggatttt atgagaaggg aaggccgcga
     1081 gccgtactcc acttcctcta tcaaaatggt gagccacgca aatggtatgt tcacttcgac
     1141 ctcttcagcc cggtctattc gccccgcagc gcagcaagtc acgtgcgcca tgaggttctg
     1201 agaaaattta ggccggactg gaaaaccatc aaacaatacg tttagctttt ttccggcgtc
     1261 aggtataaag gaaatggttt tggtgcttaa aggagcagct ttttatgttc gatcagttca
     1321 ccttgggaat tgaagaagaa tttcagattg tcgatccgaa gtctcgtgag ttgcgttccc
     1381 acgtgtctga gttcctcgag gaaggcaaga tgattctcgg cgagcagata aagcccgaga
     1441 tgattcagtc gatgattgaa gttggcaccg gcgtttgcaa aaacattcag gaagcgcgcg
     1501 ctgatatcac ccgtctgcgc agcatcatct ctggtctcgc cgggaaaacc gggctggcaa
     1561 tagtggccgc ctcgacgcat ccgttttccc actggcagga tcaaaaaatc tacgacgatg
     1621 aaaggtactc gctgttggtt caggagctac agaccgtggc gcgttcgctt ttgattttcg
     1681 gcttgcacgt tcatgttggc gtggcagact tagaccggcg gattcacatc atgaatgccg
     1741 cccgctattt cttgccccac gtcctggcgc tcacaacgtc gtcgcctttc tggatgggcc
     1801 acagtacggg acttaagtcg taccgctcgg aggttttcaa acagtttccc cgcacagaca
     1861 ttcccgacca cttcgactcc tatagcagtt ttcagcgtta cgtcgacttg cttgtgaaga
     1921 cagggtgcat aaacgacggt aaaaagatct ggtgggactt gcgtccgcat ccaattttcc
     1981 cgacgctgga gtttcgtatc tgtgatatcc caacgcgcgt tgacgacacg gtcgcaattg
     2041 ccgccctgtt ccaggcaatc gtcgccaaac t
//
LOCUS       DASUEX010000459         3812 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1123499, whole genome shotgun sequence.
ACCESSION   DASUEX010000459 DASUEX010000000
VERSION     DASUEX010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3812
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1123499"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..768
                     /locus_tag="VFV61_07755"
     CDS             <1..768
                     /locus_tag="VFV61_07755"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX4900412.1"
                     /translation="SPIAVIQGVTTIQPILPDKEHVFEVGAQQLLSRYLRLNVTLYQK
                     RIKNFSDKDQFIETGVIFPVAISSGRVTGEEIKFESTDIKGFHGSLSFANSHAYGVTP
                     ITGGLFLGEDPQELQTRGIKFSADHDQRNSAQFQFGYNHRPTGIYATFGGRYDSGVPT
                     DVEPGTTLTDFIAQGFDPRLYQEIDFQRGRVRPRTVFDLSVGADLMQKERVAMNLQFD
                     VQNLTNELFLYNFQSVFSGTHVGPPRLISGRISLRFK"
     gene            1131..2075
                     /locus_tag="VFV61_07760"
     CDS             1131..2075
                     /locus_tag="VFV61_07760"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX4900413.1"
                     /translation="MNRVLLQDPSSDDEVIRVNTELLLFPIRVRDRRKPEQPLTESDL
                     MLKDEDRVTSGLYFSAGADRLALVFALDESGSLREIIAQQREAALALFGRFGEKSRVA
                     VLRFSNSPKLVAEFGRDLEAARSAFTFPAKPNQRTAIFDAAAAAVRSFDSLPRIRSER
                     HIVILISDGLDNASTAKAENIITAALDKRVSFYVIHLPLFTPREGQLAIRSPTKGFRE
                     LAEKTGGRFFVAGDAKSALSPKPVDLTPVFQAIEDDLRGQYLVGFYLGDKANDGRKHK
                     FSLGLPEKVEYQVRSLGYSRRHEFFVERPREVLRQQRQ"
     gene            2370..>3812
                     /gene="purH"
                     /locus_tag="VFV61_07765"
     CDS             2370..>3812
                     /gene="purH"
                     /locus_tag="VFV61_07765"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: protein motif:HMM:NF002049.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEX4900414.1"
                     /translation="MTDNQQLGLRKVRRALLSVSDKTGIIEFASALKNFGVEIISTGG
                     TARTLRDAGFDVFDVSDVTGFPEMMDGRIKTLHPKIHGALLGVRNNPEHEAAMREHSI
                     QPIDLVVISLYPFQETIAHDGVTEDEAIEQIDIGGPAMIRSAAKNFRDVAVAVSPHFY
                     AEIADELANNGGALTLAKRAYLATQAFFHTAAYDRSISIYFASRLQNATESASCPTIE
                     TASDHFSITRVDKLQAFTSTIEEHGFPPHLSLMVEKQTDLRYGENPHQKAALYTDDQN
                     GGIAQAELLSGKEMSFNNFVDADAAWQLVSDFDERACAIIKHTNPAGVGLGASNEEAY
                     RRALETDPVSAFGGIVAFNQKVDEAAAKAVVEIFTEVIVAPDYHSAALDILKTKKNLR
                     VLRIPPTTGAAGLEYRQISGGMLIQTSDAHQRRADELKIVSKREPSEAELRALLFAWT
                     VCKHTKSNAIVYARVGQTVGVGAGQMSRVDS"
BASE COUNT          900 a         1014 c          990 g          908 t
ORIGIN      
        1 tcgccgattg ctgtcattca aggtgtcact acgattcaac caatcctgcc ggacaaggaa
       61 cacgtgttcg aagttggggc acagcagctc ctgtcgcgat acttgcgact taatgtcacc
      121 ctttaccaga aacgaattaa gaacttctcc gataaggatc aattcattga gacgggggtc
      181 atatttcccg ttgccatctc ttctggtcgc gtaacaggtg aagaaataaa atttgaatca
      241 accgacatca agggctttca tggctccctg tcctttgcaa attcacatgc ttatggtgtg
      301 actccgatca ctggtggctt gtttctcggg gaggatccgc aggaactgca aacccgtggt
      361 atcaagtttt ctgctgatca cgatcagaga aactcggcgc agtttcagtt tggttacaat
      421 catcgaccaa ccggcatcta cgctactttt ggaggacgct atgattcggg agtgccaacc
      481 gacgtggagc cgggtacgac actcactgat ttcatcgccc agggctttga tccccgactc
      541 taccaggaga ttgatttcca gcgcggccgc gtgcgtcccc ggacagtgtt tgatctgtcc
      601 gtcggcgctg acttgatgca gaaagagcgg gtagcaatga acctccaatt cgacgtgcaa
      661 aacctgacta atgagctgtt tttgtacaat tttcaatcag tgttcagcgg tacgcatgtt
      721 ggcccgcccc gcctgattag cgggcgcatc tcgctcaggt ttaaatagtg ggaaccggag
      781 gcggacaact tcgtaatcaa actagtgcat ttcgcatgat gaactgaggg taggggcgca
      841 gcggcaaacg acgcattgtt gacagggcca aagtcggctt ctagaatgtg cgcgttaagt
      901 ctctccaagc tgcctcctaa tggtcaccta aggaaatcac tgtgactttg gttaagatca
      961 tcgttattac agtgctgatt atcctgagca tactgtttct cgggcgtttc ttcatttagc
     1021 cgaaagggct gattcgcctg ccccccgcaa cgctcaaaag aaatctgcct gcatcgctgc
     1081 gttttgtcgc ggtcgcaatc ttcgctttaa gcctgtcaac aacactcttt gtgaacaggg
     1141 tgctgctaca ggatccgtca tccgacgacg aagtaatccg cgtcaatacc gaactgctgc
     1201 tcttccccat tcgcgttcgc gacagaagaa agccagaaca gccattaacc gagtctgact
     1261 tgatgctgaa ggacgaggac cgcgtcacta gcggtcttta cttctccgcc ggtgcggatc
     1321 gactggctct agttttcgct ctcgacgagt caggcagctt gcgcgaaatt atcgcgcagc
     1381 agcgcgaagc agcgctggca ctctttggaa ggtttgggga gaaatcacgc gtcgccgttt
     1441 tgcgcttctc taacagtccg aagctagtgg cagaatttgg acgcgacctg gaagcagcac
     1501 gtagtgcctt tacctttcct gccaaaccta atcaacgtac tgcaattttt gacgcagcgg
     1561 cggcagccgt gcgctcgttt gactcgctgc cacgcattcg ttcggagcgg catatcgtga
     1621 tcctgatcag cgacggactc gacaatgcca gcaccgccaa agctgagaac attattacag
     1681 cggctctgga caagcgcgtt tcgttctacg taatccattt gcctctgttt acgcctcgcg
     1741 aaggacaact cgccatacgt tcccccacaa aaggctttcg cgagctcgcc gagaagaccg
     1801 ggggcagatt cttcgttgcg ggcgatgcaa agtccgcact gtcaccaaag cccgtcgatt
     1861 tgacgccggt atttcaagct attgaggacg acctgcgtgg tcagtatctc gtagggtttt
     1921 atctcggtga caaggccaac gacggccgga agcacaaatt ctcgctggga ttgccggaga
     1981 aagttgagta tcaggttcga agtcttggct actcacgccg gcacgagttc tttgtagagc
     2041 ggcctcgtga agtgcttaga caacaacggc agtagcgatc gagttcagtc ccaaagcacg
     2101 ctgcctgaga gccagatttc attcacaccg gacttcagcc cggtggccag atcagaatca
     2161 cttttcgcta accgtttcaa cggttttttg gcgattggag aaaccgttaa aacggttctg
     2221 aggtgggtcg tgagacctgt caccgcgctg aagcccggtg tgaatgagac aggcatatga
     2281 atgattgtct ctctagtcgc gacttgctta ctgcttctcg attctgtaaa cagtactctc
     2341 caaatccaaa ctcagtttgt aatcaccaaa tgaccgataa ccagcaactc ggcttgcgca
     2401 aagttcgtcg cgcgctccta agcgtttcag acaagacagg aattattgaa tttgcctcag
     2461 ctctcaaaaa cttcggcgta gagataatca gtactggagg cacggcccgc actttgcgcg
     2521 acgctggctt cgacgttttc gatgtttctg acgtcaccgg ctttccggaa atgatggacg
     2581 gccggatcaa gaccctgcat ccgaagatcc atggcgcgct gctcggcgtg cgcaacaatc
     2641 cggagcatga ggcagcgatg cgggaacaca gcattcaacc aatcgatttg gttgtgatca
     2701 gtctctatcc cttccaggaa actattgctc atgatggcgt cactgaggat gaagcaattg
     2761 agcagatcga tattggcgga cctgccatga ttcgttcggc ggcgaaaaat tttcgggacg
     2821 tagcggttgc tgtgtcacca catttttatg cggaaatcgc tgacgagctt gctaacaatg
     2881 gaggggcgct gactcttgcg aagcgtgcgt atcttgccac tcaggctttc ttccacacag
     2941 cggcctatga tcgcagtatc tctatctact tcgcttcaag gcttcagaac gcgactgaaa
     3001 gcgcttcgtg tccaaccatt gaaacggcca gtgatcattt ttccatcaca cgagttgata
     3061 agctgcaagc tttcacttct accattgaag aacatggttt tccgccgcac ctcagcctaa
     3121 tggtcgagaa acaaacagac cttcgctacg gcgagaaccc acatcagaaa gctgcgctct
     3181 atactgacga tcaaaatggt ggaatcgccc aggccgaact gctctccggc aaggaaatgt
     3241 cattcaataa cttcgttgat gccgatgctg cctggcagct ggtgtcagac tttgatgagc
     3301 gtgcatgcgc aatcatcaag cacacgaatc cggcaggagt cggcctggga gcttcgaatg
     3361 aggaagccta tcgccgcgct ttagaaacgg atcctgtgtc agcctttggc ggcatcgtgg
     3421 cttttaatca gaaagtcgat gaggctgcgg ccaaggcggt tgttgaaatc ttcactgaag
     3481 ttatagtcgc gccggactac cactcagcgg ctctcgacat attgaagact aagaagaact
     3541 tgagggtact acgcatacct ccaactaccg gtgcagcagg gcttgaatac aggcagatca
     3601 gcggcggcat gctcatccaa accagcgacg cgcaccaacg gagagcagac gagctgaaga
     3661 tcgtcagcaa gcgtgaacct tccgaagctg agcttcgcgc gctcttattc gcgtggactg
     3721 tctgcaagca caccaaatca aatgcgattg tttacgcgcg cgtgggccaa acggtaggag
     3781 tcggcgccgg gcagatgtcg cgagtcgact ca
//
LOCUS       DASUEX010000460         4135 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_301099, whole genome shotgun sequence.
ACCESSION   DASUEX010000460 DASUEX010000000
VERSION     DASUEX010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4135
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301099"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="VFV61_07770"
     CDS             <1..205
                     /locus_tag="VFV61_07770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900415.1"
                     /translation="FTEPATGSPLSETEQVALNSFLAKHQGSDPAIEINDKDANRPYA
                     AVKASRADSATSPIIFRIRIQHK"
     gene            239..1135
                     /locus_tag="VFV61_07775"
     CDS             239..1135
                     /locus_tag="VFV61_07775"
                     /inference="COORDINATES: protein motif:HMM:NF025684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HEX4900416.1"
                     /translation="MRLKVLICALAVSTLAGLGTVTALTQDQEEDVRGAFLTTRPKPA
                     DKSKSNESARPNRRRPKPQAVKADPTAGGNTAGTNVKDEPNKVTPQKLGLGLTLLGRD
                     SLGLTVRIDPTRTFRKGDRVRVLLETNNDGYLYIFNTTNDGKPVMLYPNKELDEGGNY
                     LQAHIPFEIPSSTAPEERLRWLVFDEFAGNERLYFVFTRSPLPGIPLEDELIAFCNDA
                     KNTCPIQPNAELWAQLQKEMDEPLRTDSNKKYGKAQTEPERQAIERGLGLSTQEPEPS
                     LIMMSASAKSGTLVTVLDLVHK"
     gene            1180..4047
                     /locus_tag="VFV61_07780"
     CDS             1180..4047
                     /locus_tag="VFV61_07780"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing tetratricopeptide repeat
                     protein"
                     /protein_id="HEX4900417.1"
                     /translation="MKYLSASVIVLGLAAGLFLAPQPFVGSTTAASTDSAQQPDQAGS
                     ALQQGRKLLKRGQTAEALGYLQTALNLYTTSKNARGIGAAHNELGDLYLRQGQHKVAL
                     EHFQKAYQALAGAVVQEQQAGAAAGGAARMAGSDAGAATEAAATASDTDFNANLVLAK
                     IGDTNFRLGRLSEASAAYSQMRVKKPESAASRSTRRFGGLGGMLGSISTGRVDVAAPT
                     SAVISLLEIKKELDLYRTAIVFSTYELGMGRISYFNNDLEGAKKHFQNAFDTSGASLA
                     SVAKLGQTRRFRAAARISLGDVALRQGKLKEASKYYSESAKGAQEDKRLDLMWPAQRG
                     LGRSLWLQAAQEKDPKKATATRESALSNYRDALKTIETLRQGSLRADEARTTFLSTTK
                     SVFDEAASALAEMALLAATPDAPLSGKALDYAAEAFRITEQSRARSLLDLLSETDAKL
                     ITGIPIELVRRKQENLERQQEIAETLTGIVVTPGEPKKKAGDLEAELDKLQTDYDEIE
                     NQIRLASPRYAALTGGQPLSLAEVQQKVLDDKTVLLEYSLGADASYLWTADSQSLRLY
                     KLPPRLTLDNLAADLRAQLIPSRLQRRIVGIDVADPSRGLGVVSASEDSAGFVAAANA
                     VYKAAVEPASTMLGEKRLLIVADGGLNYIPFEALVKSSASADYSSLPYLIKSNEIVYA
                     PSASVIGAIRQQGAKASGRAMLIVADPVFNSNDARARTAAPSATGAETRGLGIESALT
                     DVAGQGAAASAESAKMQGLPLARLAGTRAEAEQIVKFARTAGGQPDLWLDLEASEDNM
                     QSMDVTKYRVVHIATHGLLNAERPQFTGLVLSLVGNRGGDGFLRTDEVFNLRLGSPLV
                     MLSACETGLGKEKRGEGVIGLTRAFMYAGAPTVGVSLWSVADKSTADLMTDFYKRLLA
                     TGAESTSATAAMRGAQLSLIAGKKYSAPFYWAPFVLVGEWR"
BASE COUNT          966 a         1169 c         1194 g          806 t
ORIGIN      
        1 ttttacggag ccggcaaccg gaagcccgtt atcggaaact gagcaagtgg cattaaacag
       61 cttcctggcg aaacatcagg gaagtgaccc ggcgattgag attaacgata aggatgcgaa
      121 ccggccttac gccgcagtca aagcttcgcg ggcggactca gcgacgagcc ccatcatatt
      181 tcgcattcgc attcaacata agtgatcccc aggaaaggat ttctgtaagg agaatgaaat
      241 gagactcaaa gtgcttatat gcgccctggc agtgtccacc ctcgccggtc ttggcacggt
      301 caccgcactc acccaagacc aggaggaaga tgtacgcggc gccttcctaa cgacgcggcc
      361 caagcccgct gacaagtcca aaagcaatga gtctgccagg cccaaccggc gtcggccaaa
      421 accgcaagca gtaaaagcag accccactgc cggcggaaac actgcaggca caaatgtcaa
      481 agacgaacct aacaaagtga cgccccagaa gctggggttg ggactaacac tattgggtcg
      541 cgattcgctt ggactcacgg taagaatcga cccaacgcgg accttccgca aaggcgatcg
      601 cgtgcgcgtc ttgctggaaa ccaacaacga cggttacctc tacattttca acaccacgaa
      661 cgatggcaag ccggtgatgc tttatccgaa caaggaactt gatgagggcg gaaactacct
      721 ccaggcgcat attcctttcg agatcccctc cagcactgcg ccggaagagc gtttgcgctg
      781 gctggtcttt gacgagttcg cgggaaatga acgtctctac tttgtgttca cgcgctcgcc
      841 attgccgggc attccccttg aggacgagct gatcgctttt tgcaatgacg cgaaaaacac
      901 ttgtccgatt cagcccaacg cagagttgtg ggcccagctg caaaaggaaa tggacgagcc
      961 cttgcgcacg gactcgaaca aaaagtacgg caaggcgcag actgaaccgg agcggcaggc
     1021 catcgaacgc ggcctgggct tgagcacgca agagccggaa ccttcattga tcatgatgag
     1081 tgcttcggca aagtcaggca cgctcgttac tgtgcttgac ctcgttcaca aataaacagc
     1141 catcgctgtt tgctcgaatc gatcactaag agagttgcca tgaaatactt atctgccagc
     1201 gttattgtgc ttggtttggc cgcaggtctg ttcctcgctc cgcagccgtt tgttggttca
     1261 accactgccg cgtcaaccga cagtgcgcaa caacctgatc aagccggatc cgcactgcaa
     1321 cagggcagga agctgttaaa gcgcggccag accgcggagg ctctcggcta tctgcaaaca
     1381 gcgctaaatc tctacacgac ttccaagaac gcccgcggga ttggcgcggc ccacaatgaa
     1441 cttggtgacc tctatctgcg gcagggtcag cacaaggttg ccctcgaaca ttttcaaaaa
     1501 gcttaccagg cgctagcggg cgctgtcgtt caggaacagc aggcgggtgc ggcggcggga
     1561 ggggcagcgc gcatggctgg ttctgacgca ggcgccgcga ctgaagcagc ggcgactgcg
     1621 agcgataccg atttcaacgc caatctggta ctggcaaaaa tcggcgacac aaattttcgt
     1681 ctcggtcgct tgagcgaggc gagcgctgcg tacagtcaaa tgcgcgtgaa gaaacccgaa
     1741 agcgcagcct ccagatctac acgccgcttt ggcggtctag ggggaatgct cggcagcatc
     1801 tccacagggc gggtcgatgt agcagcacca accagcgccg tcatctcgtt gctggagatt
     1861 aagaaagaac tcgatctcta tcgcacggca atcgtcttct cgacttacga actgggaatg
     1921 ggtcgtatca gctactttaa caatgacctc gaaggcgcca agaaacattt ccaaaatgct
     1981 tttgacacga gcggcgccag cctcgcgtcg gtcgcaaagt taggccaaac tcggcgcttc
     2041 cgcgcggcag cgcgcattag tttgggcgat gttgctttgc gacaaggcaa gctgaaagag
     2101 gcgagtaagt actactctga atcggcaaag ggtgcccagg aagacaaacg tcttgatttg
     2161 atgtggcccg cgcaaagagg tttgggccgc agtttgtggc tgcaagctgc ccaggagaag
     2221 gatccgaaga aagctaccgc tacacgcgag tccgcgcttt cgaactatcg cgacgcactc
     2281 aagacaattg aaacactgcg gcagggaagt ttgcgtgctg acgaagcgcg cacgacgttt
     2341 ctttctacga ccaagagcgt ctttgacgaa gccgccagcg cgcttgcgga aatggcgctg
     2401 ctcgccgcga cgccggatgc accactctcc ggaaaggctc tcgattacgc cgccgaagcc
     2461 tttcgcatca ccgaacaatc gcgcgcgcgt tcgttgctcg accttctaag cgaaactgat
     2521 gcgaagctga ttaccggcat tcccatcgaa ttagtaagac gcaaacagga gaacctggag
     2581 cgacaacagg aaattgccga aacgctgacg ggaatcgtcg taactcccgg tgagccgaag
     2641 aagaaagccg gcgatctgga agcagagctg gacaaattac aaacggacta cgatgagatt
     2701 gagaaccaga tccgtttggc gagtccgcgc tacgcagcgt tgaccggtgg gcagccgctt
     2761 tcgctcgccg aagttcagca gaaggtgctg gacgataaga cagtgctact tgaatacagt
     2821 cttggtgcag atgcctcata tttgtggaca gccgactcgc agagcctgcg actctacaag
     2881 ctgccgcccc gattgacgct ggataatctg gcggcagatt tgcgggcaca gttgatcccc
     2941 tcgaggttgc aacgtcgcat tgtcggtatc gatgttgcgg atcctagtcg cggcctgggc
     3001 gtcgtatcgg cgtcagagga ctccgccggc tttgtcgcag ccgcgaatgc tgtctataaa
     3061 gccgcagttg agcctgcgag cacgatgctg ggcgagaagc gcttactcat cgtggctgat
     3121 ggcggtctca actacattcc ttttgaggcc ctcgtcaaaa gttccgccag tgcggattac
     3181 tcgtctttgc catatctgat taaatcaaac gagattgtct atgctccatc ggcgtcagtg
     3241 attggagcaa ttcgccagca aggagccaag gcgagcggcc gcgccatgtt gatagtcgcg
     3301 gatccggttt tcaattcaaa cgatgcgcgg gcgcgaacgg cggccccctc agcaacaggt
     3361 gcggagacga ggggactcgg tatcgagagc gccctgactg acgttgcggg acaaggcgcc
     3421 gcggcctccg cggagagtgc caagatgcag ggtctgccgc ttgcccggct tgccggcacg
     3481 cgtgccgaag cggagcaaat tgttaagttc gcccggactg cgggagggca gccagatctc
     3541 tggctcgacc tcgaggccag cgaagacaac atgcagagca tggatgtgac aaagtaccgc
     3601 gttgtccaca tcgccactca cggattactt aacgccgaac gtccgcagtt tacgggtctg
     3661 gtgctttcgc tggtcggcaa cagaggtggc gatggcttcc tccgcacaga cgaggtcttc
     3721 aacctccgcc ttggctcacc tttagtgatg ctgtcggctt gcgaaaccgg tttaggtaaa
     3781 gagaagcgcg gtgagggcgt gatcggactc acccgcgctt tcatgtacgc aggggccccc
     3841 actgtcggcg tcagtctctg gtccgtggcc gataagtcga ccgcagatct gatgacagac
     3901 ttctacaaac gtctgctggc aaccggtgcc gaatcgacgt cagcaacagc ggcgatgcgt
     3961 ggtgctcagc tttcgctcat agccgggaag aaatacagcg cgccattcta ctgggctccg
     4021 tttgttctcg tcggtgaatg gcgctaaccc gctgacgcct tcttagaaac cctcccaatg
     4081 cggtcagccc ggagcctaac aggaacagcg aggctggttc tggaacaggt tccgc
//
LOCUS       DASUEX010000461         2853 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_412784, whole genome shotgun sequence.
ACCESSION   DASUEX010000461 DASUEX010000000
VERSION     DASUEX010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2853
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412784"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="VFV61_07785"
     CDS             complement(<1..254)
                     /locus_tag="VFV61_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900418.1"
                     /translation="MSTVGVDKQSADITVGLLKENELPDADRIFRLAFGTFIGLPEPE
                     EFHKGGDYIRTRWITDPNAFFAARDQERLIGSNFGTNWGSV"
     gene            complement(315..1616)
                     /locus_tag="VFV61_07790"
     CDS             complement(315..1616)
                     /locus_tag="VFV61_07790"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX4900419.1"
                     /translation="MNNRRTFLSLAGKGLGLATLSFPAVAALLKEIHGATKAVAHLSP
                     EQAATEEDYWSTIQTAFTVTRGIINLNNGGVSPSPRIVTEALVRYIWQQEDATAYTMW
                     QILEPQSETIRTGLAELFGCDREEIAITRNASESLDVLLMGMDFKSGDEILTTTQDYP
                     RMLTTLRQREKREGLVLKLIKVPIPPQNLNEITTAFERGISSRTRLILIAHQVNITGQ
                     ITPVKAVCEMARAKGIETVVDGAHSFAQFDFKQKDLGCDYFGTSLHKWLYAPKGTGLL
                     YVRREKIEKIWPLMAAETKQASDIRKFEEIGTHSAAPRLAIGEALLFHNGIGGKRKEA
                     RLRYLSRYWMNRLRDVPRIRFNTSFDPAQSCAIANVQVTGIDPTAIGSYLFNKHRIFT
                     TPIIHEEFQGIRITPNVYTTLGELDRFCDVMEQIARKGLPA"
     gene            complement(1641..>2853)
                     /locus_tag="VFV61_07795"
     CDS             complement(1641..>2853)
                     /locus_tag="VFV61_07795"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX4900420.1"
                     /translation="NPVSLTRADGHASVVNSAALKLAGIDRNTANPFGGEISKDKSGE
                     PNGMLLDAAQGLVRRRIPAASAGEAEKAIILGVTRDISLGWTQIQDAGGTFQDVELYK
                     KLYGEGKIKLRIYKSVYGPGPNAEKILYGGAITGAFGNRLNVRSIKVVSDGALGSRGA
                     ALLEPYADAPDTSGFLTVKPEELAPMLEMALKNGIQVETHAIGDRANRFILDQYEQAL
                     SKVPERERKIPNPRWRVEHAQLVNPTDIPRFAKLGIIPSMQPSHAIGDLHFAPSRVGI
                     ERLKGAYAWESFIKSGVIVPGGSDAPVERGEPMIEFYAAVARKDQKGFTGEGWHPEEA
                     VTRAEALKMFTIWPAYAAFEETLLGSIEVGKLADFTILSADIMKIPEAEILRTRNVMT
                     VISGEIVFEAK"
BASE COUNT          640 a          809 c          720 g          684 t
ORIGIN      
        1 acgctgcccc agttcgtacc aaagtttgaa ccaatcagtc tctcttgatc gcgcgccgca
       61 aagaaggcgt tcgggtcagt tatccagcgg gttctaatgt agtctccccc tttgtggaat
      121 tcttccggtt cgggcagacc aatgaaagtg ccgaacgcca ggcggaaaat ccgatcggca
      181 tccggtaact cattctcttt tagcaaccca acagtgatat cagcggattg tttgtctacg
      241 cctacagttg acacagtgtc acctcccccc ttttgagttt gcaggtcagg aatgtggttt
      301 acccccaaac gcaattatgc cggcaggcct ttacgggcga tctgctccat cacatcgcag
      361 aaccggtcca gttcacccag ggtggtatag acgtttggcg tgatccgaat accctgaaac
      421 tcttcgtgaa taatcggcgt ggtgaaaata cgatgcttgt tgaagagata gctgccaatg
      481 gcagttggat caatgccagt cacctgtaca ttcgcaattg cacaggattg ggccggatca
      541 aacgacgtat tgaatcgaat ccgagggaca tctctgagtc tattcatcca atagcgcgaa
      601 agataccgga gcctcgcttc tttgcgtttg ccaccaatgc cgttgtgaaa aagtagcgcc
      661 tcaccaatcg ccaaccgcgg cgccgctgaa tgcgtgccaa tctcttcaaa tttgcgaatg
      721 tcggaagcct gctttgtctc cgccgccatc aatggccaga tcttctcaat cttttccctt
      781 ctcacgtaaa gcagaccagt ccccttcggt gcgtaaagcc atttgtgcaa cgaggtgcca
      841 aagtaatcgc agcccagatc cttctgctta aagtcaaact gcgcaaaaga atgtgcgcca
      901 tcaaccaccg tctcaattcc ctttgcgcgt gccatttcgc aaacagcttt caccggagtg
      961 atctgaccgg tgatattcac ctgatgagca atcaggatca ggcgagtgcg actgctgatg
     1021 ccgcgttcaa aggcagtagt gatttcgttt agattttgag gcgggatggg cactttgatc
     1081 agcttcaaca ccagcccttc tcgcttttcc cgctggcgca aagtggttag catgcgcgga
     1141 tagtcctgcg tagttgtcag gatttcgtcg ccggatttga agtccatccc catcaacaat
     1201 acgtccagag actcagaggc gttgcgcgtg atcgcaatct cctcgcgatc gcagccaaag
     1261 agttcagcca aacccgtgcg aatggtttca gactggggct cgagtatctg ccacatcgtg
     1321 taggcagtcg cgtcttcctg ctgccagatg taacgaacga gagcttcagt gactatgcgt
     1381 gggctgggcg acacaccacc gttattcagg ttgataattc cgcgggtgac ggtgaaggca
     1441 gtctgaatag tggaccagta gtcttcctca gtcgcagcct gctccggact gaggtgtgcg
     1501 acggcctttg ttgctccgtg gatctctttc agcaatgcgg cgacagccgg aaacgaaagg
     1561 gtggcgaggc caaggccctt accggcaagc gagagaaaag tgcggcgatt gttcatgttg
     1621 gttctcacta ctcgggccgg ctacttcgct tcaaacacga tttcaccgct aatgactgtc
     1681 attacgtttc gcgttcttaa tatctccgct tccgggatct tcatgatgtc cgccgagagg
     1741 atcgtgaaat cggctaattt cccaacttcg atggagccga gcagagtctc ttcaaaggct
     1801 gcgtaggccg gccagattgt gaacatcttc aaggcctcag ctcgtgtgac ggcctcctcc
     1861 ggatgccatc cctcaccggt aaaacctttc tggtccttgc gggcgacggc cgcataaaac
     1921 tcgatcatcg gctccccacg ttcaaccgga gcatcggaac cgcccggcac gatgacaccc
     1981 gacttgatga aactttccca ggcgtaagcg cccttcagcc gttcaatccc cacccggctc
     2041 ggcgcaaaat gcagatcgcc aatcgcatgc gacggctgca ttgaagggat gatgcccagc
     2101 tttgcgaagc gtggaatatc ggtagggtta actagttgcg cgtgctcaac gcgccaacgt
     2161 gggttcggga tcttacgttc acgctccggc accttactca acgcttgttc gtattgatca
     2221 agaatgaaac gattggcgcg atctccaatc gcatgggttt caacttgaat gccgttcttc
     2281 agcgccatct caagcatcgg cgcaagctcc tctggcttta cggttagaaa tccagaggtg
     2341 tcaggagcat cggcgtaagg ctccaacaac gccgcgccac gcgaacctaa tgctccgtcg
     2401 gagaccacct tgatcgacct cacgttcaga cgatttccaa acgcaccagt gatcgcaccg
     2461 ccatacaaga tcttttcggc atttggaccg ggaccataaa ccgatttgta gatgcgaagc
     2521 ttgatctttc cttcaccata aagtttcttg taaagctcga catcctggaa cgtcccacct
     2581 gcgtcctgga tctgcgtcca gccaaggctg atgtctctag tgacacccaa gattattgct
     2641 ttctcagctt ctccggccga cgcggccgga attcgacggc gtacgagtcc ctgcgcggcg
     2701 tcaagcaaca taccgttcgg ctcgccactt ttgtcttttg aaatttcacc gccaaaggga
     2761 tttgctgtgt ttctgtcgat tccagcaagt ttcagggcgg cagagttgac caccgaggca
     2821 tgaccgtcag cacgagtaag actcaccgga tta
//
LOCUS       DASUEX010000462         3250 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1124255, whole genome shotgun sequence.
ACCESSION   DASUEX010000462 DASUEX010000000
VERSION     DASUEX010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3250
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1124255"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(329..2617)
                     /gene="purL"
                     /locus_tag="VFV61_07800"
     CDS             complement(329..2617)
                     /gene="purL"
                     /locus_tag="VFV61_07800"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HEX4900421.1"
                     /translation="MITPEVISAHNLTLEEFERIKSLLGREPNLVELGIFSVMWSEHC
                     SYKSSRIHLKRLPTTGERVIVPPGENAGVVDVGDGWCAAFKIESHNHPSFIEPFQGAA
                     TGVGGILRDVFTMGARPIASLNSLRFGSLDHPQYGRRNRSLLAGVVAGIAHYGNAFGV
                     PTIGGEVAFDDAYTFNPLVNAFALGLVRQDQIFFGKATGVGNPVLYVGAKTGRDGIHG
                     ATMASAEFDDEALEKRPTVQVGDPFLEKLLLEACLEAMRSGAVAGIQDMGAAGLTSSS
                     CEMAARAGTGIELDLSLVPQREDGMTPYEMLLSESQERMLIVAHSGREREVMDIFRKW
                     DLDAVVIGRVREGQRMLVLHNGETVADIPVSLLTDEAPKYERPLLSPRSNVQSPTSAS
                     ETSKTKGQSSKTIDHNEALLSLLACPNLASKQWVYRQYDHMVRTNTAVLPGADAAVVR
                     IKETRRALAMTLDGNGRYCGANPRAGAKLIVAEAARNVVCVGARPLAITNCLNFASPE
                     RPEVMWAFSEVIDGMAEACAAFNTPVVSGNVSFYNETEGRGILPTPVIGMVGLIEDVH
                     RVVQPGFKREGELIALLGTTADDLTLSEYAVTVAGQSVSELIASGKVPEVDLKLEKAI
                     QDTCLEAAEAGLLQSAHDCSDGGLAIALAESCFSSLNREAIGADLNLTGVLPVPTLLF
                     AESPSRIVISFDEAKRDHVAALAAKHNVPLAVLGSVGGKNLSILVNGEPIIAQPVHEL
                     ENAWRNSLPQKLEAQAMAAGRE"
     gene            complement(2859..>3250)
                     /locus_tag="VFV61_07805"
     CDS             complement(2859..>3250)
                     /locus_tag="VFV61_07805"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX4900422.1"
                     /translation="TKNKPLLVNFWATWCDPCREEFPDLVKIDKQYRPQGLDFTAVSI
                     DDLSEIKTTVPQFLREMQVTVPTYLLNVSDPEVAMSRVDPQWSGALPATFLYDAQGNL
                     VYKRLGRIKVDELTAAIDKLVSKTGDQ"
BASE COUNT          733 a          918 c          876 g          723 t
ORIGIN      
        1 gatcgccacg gtggtgaaga cgccgcgacc gtataacatt gcggaagaga cgggtgccac
       61 acgtgccttt gtggcggcca gcattgtgcg attgaggtag atgacgggat gcacgaccta
      121 gttgtggcag ttactcaata cgctacatga gcggtaaaga tctatcattt ctcagttctc
      181 atttgggtta ctgtgcttca ttttgattgg tctttggttc gtgaatcaca cgaactggcc
      241 cgcggaacaa gaacagccac ttgaccccaa atgaaaaatg aaaactgaga aatgatatat
      301 ggaaaatgat ctttaccgca gcagctcgct attccctccc tgcggccata gcctgtgctt
      361 ccagtttctg cggtaaagaa ttgcgccagg cattctccag ctcgtgaact ggctgagcga
      421 tgatcggctc gccgttgacc aggatcgaga gattcttccc gccgacgctt cccagaactg
      481 ccaaaggaac gttgtgtttc gctgccagcg ccgcaacgtg atcgcgtttc gcttcgtcaa
      541 aactgatcac aatgcgggag ggtgattcag caaataacaa agtcggtacc ggcagaactc
      601 ccgtcaggtt cagatcagcg ccaatcgcct cgcggttcaa tgaagagaaa caggattcag
      661 cgagtgcgat agcgaggccg ccatcggaac aatcgtgcgc tgattgcagc agcccggctt
      721 cagcagcttc caaacaggtg tcttgaatcg ccttctccag tttcagatcc acctcgggaa
      781 ctttgccact ggcaatcaac tccgatacgg attgtcctgc gacggtcacg gcatattcgc
      841 taagcgtgag atcatcggcc gtggttccta aaagtgcgat caattcacct tcgcgtttga
      901 atcccggttg cactacgcga tggacgtctt ctattaaacc taccattccg atcactggag
      961 tcggcaggat gccgcggccc tcagtttcgt tatagaacga gacgttgccg gagacgaccg
     1021 gagtgttgaa ggcagcgcag gcctcagcca tgccgtcgat cacttccgaa aaagcccaca
     1081 tcacttcagg acgttcaggc gaggcgaagt ttagacaatt ggtgatcgcg agtggccgcg
     1141 cgcccacgca gacaacattg cgcgcggctt cggcgacaat aagctttgcg ccggcacgcg
     1201 gatttgcgcc gcaatagcgg ccgttgccgt ccagtgtcat tgcgagtgcc cggcgcgtct
     1261 ccttgattcg caccaccgca gcatcagcgc caggcaacac ggcagtgttg gtgcgcacca
     1321 tgtgatcata ctggcgatag acccattgct tggaagcgag gttcggacac gcaaggaggc
     1381 tcagcaatgc ctcgttgtgg tcgatggtct ttgaactttg gcctttggtc tttgaagttt
     1441 cgctcgcgga cgttggactt tggacgttgg accttggact aagtagtggt cgttcgtatt
     1501 tcggcgcttc atcagtgagt aatgaaaccg gaatatccgc cactgtttca ccgttatgga
     1561 gcaccagcat ccgctggccc tcacggacgc gtccgattac caccgcatca aggtcccact
     1621 tgcgaaagat gtccatcacc tcgcgctcgc gtcccgagtg ggccacgatt agcatgcgct
     1681 cctgcgattc cgagagcagc atctcgtaag gagtcatgcc gtcttcgcgt tgcggtacga
     1741 gcgagagatc caattcaatc ccagtcccgg cccgcgccgc catttcacag gaggaagaag
     1801 ttagtccggc cgcccccatg tcctgtatgc cggcaactgc accgctgcgc attgcttcca
     1861 gacaagcctc gagcaacagc ttttccagga acggatcgcc cacttgaact gtaggacgct
     1921 tctcgagagc ctcatcatca aactccgctg atgccatggt cgcgccgtga atgccgtctc
     1981 gacctgtctt cgctcccacg tacagaaccg gattgccgac tcccgtggct ttgccgaaaa
     2041 agatttgatc ctggcgcacc agccccagcg cgaaggcatt cacgagcggg ttgaaagtgt
     2101 aagcatcatc aaaagctact tcaccaccga tcgtgggcac gccaaaagcg ttgccgtaat
     2161 gcgcgatacc ggccacaacg ccggcaagta gcgagcggtt acgccggcca tactgtggat
     2221 gatccaaaga gccgaagcgc aggctgttca gcgaagcgat gggacgcgcg cccatcgtaa
     2281 acacgtcgcg caggatgccg ccgacgcctg tggccgcgcc ctgaaatggc tcgatgaatg
     2341 acggatggtt gtgcgattca atcttgaagg cagcgcacca accgtcgcct acgtccacaa
     2401 cgccggcatt ttccccgggc gggacgatca ctcgttcccc ggtagtgggc agacgcttca
     2461 aatggatgcg agatgatttg taggaacagt gctccgacca catcaccgaa aagattccca
     2521 gctcaaccag gttcggttcg cggcctaaga gtgatttgat tcgctcaaat tcttcgagag
     2581 ttaagttgtg tgcggagatt acttcgggag tgatcatttg gtgatggtgt caggtatggg
     2641 tgtcagagcg ttggcaatac aggtcccaag tctcaggtct caaaaacgcc atgacgccaa
     2701 ggcgccaagt cgcaaagacg caaagaatga tctaatagct cgctaacccc ttccggtatc
     2761 ggtttgattc gtcgtaccta accctaacca ctcaacctgc ttgcaaagct tcctttgcgc
     2821 cttgggtctt cgcgacttgg cgttggaaca gacactgact actgatcacc cgtcttgcta
     2881 accagcttat caatcgcggc ggtcagttca tcaaccttaa ttcttcctag ccgcttatag
     2941 acaagattgc cctgtgcgtc atagagaaac gttgccggca aggcgccgct ccattgggga
     3001 tcgactctgc tcatcgccac ttcgggatca ctgacattca aaaggtaggt cggaacggtg
     3061 acctgcattt cgcgcagaaa ctgcggcaca gtggtcttaa tctcgctgag atcgtcgatt
     3121 gagacggcag tgaaatcgag tccctgcggc cggtactgct tatcgatctt caccaggtcg
     3181 ggaaactctt cacggcaggg atcacaccag gtggcccaaa aattaacaag gagaggtttg
     3241 ttctttgtcg
//
LOCUS       DASUEX010000463         3136 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1216209, whole genome shotgun sequence.
ACCESSION   DASUEX010000463 DASUEX010000000
VERSION     DASUEX010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3136
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1216209"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="VFV61_07810"
     CDS             complement(<1..283)
                     /locus_tag="VFV61_07810"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX4900423.1"
                     /translation="MRKTIGDRQSPIGNEKMLTLENVSVNYGAIEALNGISMHVEKGE
                     VVTLIGANGAGKTTTLRTITGLLEPRAGKIIYEGEDISAKPTHKLVARGI"
     gene            complement(348..1139)
                     /locus_tag="VFV61_07815"
     CDS             complement(348..1139)
                     /locus_tag="VFV61_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4900424.1"
                     /translation="MSNNVTSNNGKPAILTTDKATIRFGGLVAVNELDMAVRSHEIYG
                     LIGPNGAGKTTIFNLLTGVYKPTSGAIHFNDKRIDGMRPYEIAGRGVSRTFQNIRLFP
                     SMTVLENVMTACHIHMGQNLLDAVLRSPRFIKDEAKHRDYCMELLAIFDLDRFKDEGG
                     TSLPYGSQRRLEIVRALATRPQLLLLDEPAAGMNPSEQEDLMNLIHEIRDRFSLTILL
                     VEHNMKVVMGVCERIQVVDYGKSIALGLPEEIKNDPKVIEAYLGD"
     gene            complement(1136..2230)
                     /locus_tag="VFV61_07820"
     CDS             complement(1136..2230)
                     /locus_tag="VFV61_07820"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX4900425.1"
                     /translation="MPRYLRNLIVILVVIGILYGLDTLFQGRAISPYFSRILMLSGIA
                     VTLAVSLNLINGFTGQFSIGHAGFMAVGAYSSAYFSVNYGANLAAALGNGKLGWVIAL
                     AIATLIGALFAGLAGLIVGVPSLRLKGDYLAIVTLGFGQIIVVFLNNIEAVGGARGYS
                     GIPIVKSFFWIFLIAILTITIVYNIVNSAFGRALISIREDELAAEAMGVNTTRYKVMA
                     FVISSAMAGAGGVLLAHFDGYLNPKSFEFIKSFEILIMIILGGLGSIVGSVLGAILLT
                     VLPEALRGFAEYRMVIYSLLLIILMITRPQGLLGKTAAFKGWRARRKTKSRTEAEEIT
                     AEDRSAGNTLDKATLGDRQSETSLGEGDEK"
     gene            complement(2330..>3136)
                     /locus_tag="VFV61_07825"
     CDS             complement(2330..>3136)
                     /locus_tag="VFV61_07825"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX4900426.1"
                     /translation="NGLGARGAQAIGMSADGLIARGLLGGGALEPSLITALVVMLLAM
                     GICAALGIVIERFAYRPVRKYSRLTALITAIGVSLLLENGGQVVFGADPKFFPELFKK
                     RNIDLFGGASINSADIIVLVIAIALMIALQLIVFRTKTGRAMRAVSFNLQSAKLMGIN
                     TDRIIMFTFALGSALAAAAGVLVAIRIPRIDPLMGILVGLKAFVAAVLGGIGNIPGAV
                     LGGLLIGITETMVVGYLSPTYKDAVAFAILILILLFRPSGLLGAVAQEKV"
BASE COUNT          765 a          885 c          816 g          670 t
ORIGIN      
        1 aaatcccgcg cgccacaagt ttatgggtcg gcttcgcgct gatgtcttct ccttcataaa
       61 tgatctttcc ggctcgtggc tcgagcaggc cggtgatcgt gcgcagcgtg gtcgtcttgc
      121 cggcgccatt agcgccgatc agcgttacca cctcaccctt ttctacgtgc atcgaaatgc
      181 cgttgagggc ctctatggcg ccgtaattaa ccgatacatt ctctagtgtg agcatcttct
      241 cattgccgat tggagattgc cgatcgccga ttgtttttct caagaaagca ggacgttacg
      301 tgaactcgac cgccaattgg caattggcaa tcgacaattg gcaatcgtta atcgcccaga
      361 tacgcttcaa tgaccttcgg atcgttcttg atttcttccg gcagtcccaa cgcgatcgat
      421 ttgccataat cgacaacttg aattcgttcg cagacaccca ttacgacctt catgttgtgt
      481 tcgaccagaa gaatcgtcaa cgagaagcga tcgcggatct cgtgaatcag attcatcagg
      541 tcttcctgct ccgaaggatt cattcccgcc gccggttcgt ccagtagcag caactgtggc
      601 cgcgtggcaa gcgcgcgcac aatttccagg cgtcgctgac tgccataagg caggctggtg
      661 ccgccctcat ccttaaaacg atccagatca aatatcgcca acagctccat gcaatagtcg
      721 cgatgctttg cttcgtcttt gatgaaacgc ggactgcgca gcaccgcatc aagcaggttc
      781 tggcccatat gaatgtgaca agccgtcatc acattttcca gaacggtcat actggggaaa
      841 aggcgaatgt tttgaaacgt gcgcgaaact ccgcgaccgg caatctcata tggccgcatg
      901 ccatcgatgc gcttgtcgtt gaaatgaatc gcaccagaag tcggtttata aacccccgtc
      961 agcaaattga agatggtggt cttgccggcg ccgttaggtc caatcaaccc atagatctcg
     1021 tgtgagcgca ccgccatatc gagttcgttc acggccacga ggccgccgaa gcgaattgtt
     1081 gctttgtcag tcgtcaggat ggcgggtttc ccgttattgg aagtgacgtt gttactcatt
     1141 tctcgtctcc ttccccgagg gaagtctcgc tttgccgatc gccaagcgtg gccttgtcca
     1201 gagtgttgcc ggcgctccga tcttccgcgg ttatttcttc agcctcggtc cgcgatttgg
     1261 ttttgcgacg cgcacgccaa cccttaaacg ccgcagtctt gcccagcaga ccttgcggcc
     1321 gggtgatcat caggatgatc agcagcaggg agtagatcac catgcgatac tcggcaaagc
     1381 cgcgcagcgc ttccggcaag acggtcaaaa gaatcgcccc cagcactgag ccgacgatgg
     1441 aaccgagtcc gccgagaatg atcatgatca ggatctcgaa cgacttgatg aactcgaacg
     1501 atttgggatt cagataacca tcgaagtgtg ccagcagcac gcctccggcc ccggccatcg
     1561 ctgaagaaat aacaaacgcc atcaccttgt agcgcgtcgt gttgacgccc atcgcctcgg
     1621 cggcaagttc atcctcgcga atcgaaatca gagcgcggcc gaaggcggaa ttgacgatgt
     1681 tgtaaacgat ggtgatggtg agaatcgcaa ttagaaaaat ccagaagaag cttttgacga
     1741 tcgggatgcc tgagtaaccg cgcgcgccgc ctactgcttc gatgttgttc agaaagacca
     1801 cgatgatctg tccaaagccc agggtcacaa tcgccagata gtcgccttta agtctgagcg
     1861 agggcacacc aacaatcaga cccgccagtc cggcaaagag agctccgatg agggtcgcaa
     1921 tagccagggc tatcacccat cccagtttgc cgttaccgag ggcagcggcc aggttcgcgc
     1981 cgtaattgac agaaaagtat gcagacgagt aggcgcccac ggccataaac cccgcatgac
     2041 cgatcgagaa ttgccccgta aaaccgttga tcagattgag tgaaaccgcg agcgtaaccg
     2101 cgatcccgga aagcatcagg atacgactaa agtaaggact aatcgcgcga ccctggaaga
     2161 gagtgtccag gccatagagt atgccgatta cgaccagaat gacgattagg ttacgtaggt
     2221 aacgaggcat gcgaatcaaa cgatgaacga tgaatgttga acgatgaata gcaagacgaa
     2281 cattagtctt aacaattcat cgttcatcat tcatcattca gcgttgcttt tagacttttt
     2341 cctgtgcgac cgccccgagt aggcccgagg gtcggaagag tagtatcaag atcaaaatgg
     2401 caaaggcgac cgcgtctttg tacgtcggcg agagataacc gacaaccatg gtttccgtaa
     2461 tgccgatgag caaaccaccg agcacggcgc cggggatgtt cccaattccg cccagcaccg
     2521 ccgccacgaa cgctttcaaa cccacgagta tgcccatcaa aggatcgatg cgcggaatgc
     2581 gaatcgcaac taacacacct gccgccgcag ccagcgccga gcccagcgcg aaggtaaaca
     2641 tgatgatgcg atcggtattg atgcccatta gtttggcgga ttgtaagttg aatgaaaccg
     2701 cgcgcatggc gcgtccagtt ttggtgcgga agacaattag ttgcagcgca atcatcaggg
     2761 caatcgcgat caccagcact atgatgtctg cactattgat gctcgctcct ccgaacaagt
     2821 caatgtttcg tttcttgaaa agttcgggaa agaatttcgg atcagcgccg aagaccacct
     2881 gaccaccgtt ttccagcagc agcgaaacgc cgattgccgt gatcaatgca gtgagccgcg
     2941 aatacttgcg caccggcctg taggcgaagc gttcaatcac gatgcccagg gcagcgcaaa
     3001 tccccatcgc caggagcatt accacgagcg cggtaatcag cgaaggctcg agcgcgccac
     3061 cgcccaacag tccacgtgca atcaatccgt ctgcactcat gccgatggct tgcgcaccgc
     3121 gggctccgag tccatt
//
LOCUS       DASUEX010000464         4178 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_761022, whole genome shotgun sequence.
ACCESSION   DASUEX010000464 DASUEX010000000
VERSION     DASUEX010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4178
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_761022"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            329..487
                     /locus_tag="VFV61_07830"
     CDS             329..487
                     /locus_tag="VFV61_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900427.1"
                     /translation="MADDGGGGGSAGVVAIVAIFVIIILVGLFMFRGRLFGGGTQKID
                     VNVQTPSR"
     gene            606..2060
                     /locus_tag="VFV61_07835"
     CDS             606..2060
                     /locus_tag="VFV61_07835"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009252629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HEX4900428.1"
                     /translation="MSAPNIETIVSLAKRRGFVYPSSEIYGGLGSAWDYGPLGTELCN
                     NVKQAWWRWMVYERDDMEGLDSSIILNRLVWKYSGHEDTFSDPLIDCRKCNSRYRADK
                     LMEESGKAAADLGNVANIKCPNCGAADWTEPRPFNLMFRTHVGATAEEDESSLVYLRP
                     ETAQGIFINFANILTTSRRKLPFGVAQIGKSFRNEVTPGNFIFRTREFEQMEMEYFVR
                     PGEDEQVHQEWIDNCQQWFLSLGISADNLRLYEQPPEELAHYAKRTVDIHYRFFPERE
                     EAERQWDELMGIANRTDFDLRTHSKKPENSEGKRLNPDSTEDLSYFDEATKERFYPYV
                     IEPAAGVNRTVLALLMDAYSERTNDKGETRVILKLHPGLAPIKAAILPLAKNKPEIVG
                     MAKAIKRSLQPTLRAVYDDTGGIGKLYARQDEIGTPFCVTVDHQSLEDESVTVRDRDT
                     WEQDRVKVSELSEYLQQRLTTVQSPRSNVQGLVP"
     gene            2138..2500
                     /locus_tag="VFV61_07840"
     CDS             2138..2500
                     /locus_tag="VFV61_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900429.1"
                     /translation="MPVVKKRNLQTETRLLLEGDDPVELPPAEIEDRNVLVQFDKFKP
                     FIGELLEELRDGTVAEPLPVMNDGSTFAIHGETRTFWVKLWHAAGTDMTKIDRAQIIS
                     CIPSTRGIQVLVEPLDDD"
     gene            2600..4003
                     /locus_tag="VFV61_07845"
     CDS             2600..4003
                     /locus_tag="VFV61_07845"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX4900430.1"
                     /translation="MSNPPEKVINLASSIRAAGGRALLVGGCVRDLLMGVAPKDWDLE
                     VYGLPPAKLREVLDSFGNVNVVGEAFSVYKLDHYLDVSIPRRDRKSGKGHRGFVIEGD
                     PDMDPAEAAQRRDFTINAIMQDPLTDEIIDPHQGRADLKNGILRAVSADTFAEDSLRV
                     LRAAQFAARFEFKVEPETETLCRDIDLSDLPAERIWGELEKLLLKAQRPSIGFNFLRS
                     TGALAQLFPEVTALIDVPQDPEWHPEGEVFTHTMLVLDRARELIDGLPYAKQVTVMLA
                     ALAHDFGKPATTEFIEERWRSRGHEEAGMAPTQSFLDRLNLHSLAGYDVRAQVIALVR
                     DHLKPGEFYKKRAEVGDGAFRRLARKCEPDLLYRVARADSLGRNSESVPREQWYGAEA
                     QEWFIERVRELNIEQGPPPPLLMGRHLLALGLAPGPRIGEITQAVYEMQLNNSVRTLE
                     EAIAAAQKLLGPTQPGN"
BASE COUNT          970 a         1075 c         1177 g          956 t
ORIGIN      
        1 gtcaagtcgc aagtctcaag tctcgagttt cgggacttga gacttttttg gtgacgggtg
       61 tcgagagacg ggtgtcaggc atcacgtgcg gcgtttccgg tgggggtggg gccgcaaagc
      121 taattaccac caaccgccac ccgacaacgg acacctaaca cccgtaaccc gacacttgag
      181 acttgagacc tgagacttga gacctgagac ttgagtcctg agacttggga cttgagactt
      241 ctcccccctt gtggtaacta cagagctaca acttcccccc aattaacgcc cccttttctt
      301 caggttcatt taacaggagg atgccttcat ggctgatgat ggaggaggtg gcggaagcgc
      361 cggcgtcgtg gcgattgtcg ccatcttcgt gataataatt ctggtgggac tgttcatgtt
      421 ccgcggacga ttgttcggcg gcggcacgca gaagattgac gttaacgtgc aaacacccag
      481 tagataacca gtaacctatt cagtttttca aagacgagga gcgtgatctg cgctcctcgt
      541 tgcttgttcg cgatcccaaa gcacccgttt actaatttga gttcagcctt tacgattatc
      601 cgcctatgtc agcacccaac attgaaacca ttgtctccct cgccaagcgg cgtgggtttg
      661 tttatccctc gtcggagatc tatggcgggc tggggagtgc ctgggactac ggtccgctgg
      721 gcacggaatt atgcaataac gtgaagcagg cctggtggcg ctggatggtc tacgaacgag
      781 acgacatgga agggttagac tcatcgatta ttttgaatcg cctcgtttgg aaatactccg
      841 gacatgaaga cacatttagc gatccgctga tcgactgccg caagtgcaat tcgcgttatc
      901 gcgctgacaa gctgatggaa gagtcaggta aagccgccgc cgacttaggc aacgttgcaa
      961 acatcaagtg tccgaactgt ggcgccgccg actggaccga gccgcgtccg ttcaatctca
     1021 tgtttcgcac tcacgtggga gcaacggcgg aggaagacga gtcgtcgctg gtatatctgc
     1081 gtccggaaac ggcccaggga atcttcatca acttcgccaa tattctgaca accagccggc
     1141 gaaaacttcc tttcggcgtt gcccagattg gcaaatcgtt tcgcaatgaa gtaacgccag
     1201 ggaattttat ttttcgcacg cgtgagtttg agcagatgga gatggagtac ttcgtgcgtc
     1261 ccggcgaaga cgagcaagtt catcaggaat ggatcgacaa ctgtcagcag tggttcttga
     1321 gtctgggaat ttcagccgac aatttgagat tgtatgagca gccgccggag gaattggcgc
     1381 actatgccaa gcgaactgtg gacattcatt atcgcttctt cccggaacgc gaagaggccg
     1441 aaaggcaatg ggatgagctc atggggatcg ccaatcgcac ggattttgat ctccgcacgc
     1501 attcgaagaa accggagaac tcggaaggca agcggcttaa tcctgactcc accgaagatc
     1561 tctcttattt tgatgaagcc acgaaggagc gcttctatcc ctacgtgatc gaaccggcgg
     1621 cgggcgtaaa ccgcaccgtg ctggcactgc tgatggatgc ttactccgag aggacaaacg
     1681 acaaaggcga aacccgggtg attctcaagc tgcaccctgg cctggcgccg atcaaagctg
     1741 cgattctgcc gcttgcgaag aacaagcccg agatcgtcgg catggcgaaa gcaatcaagc
     1801 gatctctgca acctacgctg cgcgcggttt atgacgatac tggtgggatc gggaagcttt
     1861 acgcgcggca ggatgagatc ggcacaccat tctgcgtgac ggtagatcac caatcattag
     1921 aggacgagtc agtcaccgtg cgtgatcgtg acacgtggga acaggaccga gttaaggtct
     1981 cggagttgag tgaatatctt cagcaaaggc tcacgacggt ccaaagtcca aggtccaatg
     2041 tccaaggtct ggttccgtga gttttctgct gccgctttgg aagcatctaa ttccccggag
     2101 ttgtaagaat ttctacgctg cggaacatta gagttttatg cctgtagtca aaaagcgaaa
     2161 cctgcaaaca gaaacgcgtt tactgctgga gggcgacgat ccggtcgagc tgccgcctgc
     2221 tgaaatcgag gaccgcaacg tcctggtgca gtttgataag ttcaagccgt tcattggcga
     2281 gttgctggaa gaattgcgcg acggcacagt tgctgagcct cttcccgtca tgaacgatgg
     2341 ctccacattc gcgattcatg gtgagactcg tactttctgg gtcaagcttt ggcatgcggc
     2401 gggaactgac atgacgaaga ttgatcgggc ccagatcatt agttgcatac cctccacccg
     2461 cggaattcag gttttggttg agccactcga cgacgactaa agtcgcaaga ctcagctctc
     2521 aagtctcacg tctgcggatc taaaacctga aacctgaaac ctgaaacctg agacccgaga
     2581 cccgagaccg aagactttca tgtctaatcc tcccgaaaaa gtaatcaatc ttgccagttc
     2641 gattcgcgcc gccggtggcc gtgcgttgct ggtcggcggg tgcgtgcgcg atttgctcat
     2701 gggcgtcgcg cccaaggatt gggatcttga agtctacggg ttgccaccgg cgaaactgcg
     2761 cgaagtactc gattcgttcg gcaatgtgaa tgtggttggt gaagccttca gcgtctataa
     2821 gctcgaccat taccttgacg tttcaattcc gcgccgtgac cgcaagtcgg gcaaaggcca
     2881 ccgcggtttt gtcattgaag gtgaccctga tatggatccg gcggaagcgg cccaacggcg
     2941 tgatttcacc atcaacgcga tcatgcagga tcctctgacc gatgagatca tcgatccgca
     3001 tcaaggtcga gcagatctga aaaatggtat cttgcgggca gtttccgccg acacgtttgc
     3061 ggaagacagt ctgcgagttt tgcgggcagc tcagtttgcc gctcgtttcg agttcaaggt
     3121 ggagcctgaa actgaaacgc tgtgccgcga tattgattta agtgatcttc ccgcggaacg
     3181 aatctgggga gagctggaaa agctcttgtt gaaagcgcag cgtccctcga ttggtttcaa
     3241 tttcctgcgc agcaccggag cactcgcgca actctttccc gaagttacag ccctcatcga
     3301 cgtgcctcaa gatcctgaat ggcatccgga aggcgaagtg tttacgcata ctatgttggt
     3361 gctcgaccgt gcgcgcgagt tgatcgatgg cttgccctat gccaaacagg taactgtaat
     3421 gctggcagcg ctcgctcatg attttggcaa accagctact acggagttca ttgaagagcg
     3481 ctggcgctcg cggggtcacg aagaagccgg tatggcgccg acccagagtt ttcttgaccg
     3541 cttgaacctg cattcgctcg ctggctatga cgttcgagct caggtgatag cgctcgtgcg
     3601 cgaccacctg aagccgggtg agttctataa gaagcgcgct gaagttggtg acggtgcttt
     3661 ccgccggctc gcgcgcaagt gtgagccgga tctgctttac cgcgtggcgc gtgcggattc
     3721 gctcgggcgc aatagtgaat cagtgccgcg ggaacaatgg tacggcgctg aggctcagga
     3781 gtggtttatt gaacgggtgc gggaattgaa tattgaacag ggaccgccac cgccgttgtt
     3841 aatgggaagg catctgttgg cgctggggtt ggcgcccggt ccaaggatcg gcgaaattac
     3901 gcaggctgtt tacgagatgc aactgaataa tagtgtccgc acgctcgaag aggctattgc
     3961 agccgctcag aaacttctag gacctactca gccaggtaac tagcagaaga gttttcagcc
     4021 tacttgagaa ttcattacaa gatccttgac ttacatgggt gaagctatta tttaagcaac
     4081 tacgaacaac ttcctcgctc acgttttttc caagtcctca aaatcaccga ccgcacaaca
     4141 cctggcggaa ccgaaggagt agttatgacc acgaaatc
//
LOCUS       DASUEX010000465         2224 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1579851, whole genome shotgun sequence.
ACCESSION   DASUEX010000465 DASUEX010000000
VERSION     DASUEX010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1579851"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..965)
                     /locus_tag="VFV61_07850"
     CDS             complement(<1..965)
                     /locus_tag="VFV61_07850"
                     /inference="COORDINATES: protein motif:HMM:NF012407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HEX4900431.1"
                     /translation="MKHTITLIPGDGIGPEVASNVVRIIEAAGVEVVWETHYAGAQAL
                     EKFGTTLPNELLDSITRNKVALKGPITTPVGKGFASVNVGLRKTLDLYANLRPVRALP
                     NIPCRYPELDLVIVRENTESLYAGIEHVVVPGVVESIKIITEKASTRIAKFAFEYSQR
                     EGRKKITAVHKANIMKLSDGLFLDCFRKVAEGFPEIIADDKIIDNACMQLVMRPEQFD
                     VMLLENLYGDIVSDLCAGLIGGLGLVPAANIGERGSVFEAVHGSAPDIAGQGLANPTA
                     LLQSGILMLRYLDEREAADRIEKAMLQVFTEGKVRTRDIGGTSKTA"
     gene            1132..>2224
                     /gene="thiO"
                     /locus_tag="VFV61_07855"
     CDS             1132..>2224
                     /gene="thiO"
                     /locus_tag="VFV61_07855"
                     /EC_number="1.4.3.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016647 - oxidoreductase activity, acting
                     on the CH-NH group of donors, oxygen as acceptor [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine oxidase ThiO"
                     /protein_id="HEX4900432.1"
                     /translation="MDERADVVVIGGGVVGLSVARSLALKQVGSVLLLERGQLGREAS
                     FAAAGMLAPQAEANTDDAFFQLACRSRDLYQAFAAQLLEETGIDVELDRTGTLYCAFT
                     EHDCTEIAARFAWQRSRGLPVEQLTAGEARELEPNLAPNLLRALKFPLDTQVENRRLL
                     SALIAANEKLGVKSRVNTTVESLRIERGQVAGVITSQGFLNTRNVVIASGAWTSFIRI
                     ESAAPRIRIEPVRGQMLCFDAKPALARHVIYSPRGYLVPRRDGRLLAGSTTEKAGFAK
                     EVTAKGLESIRTRALEIVPGVAGLTLVDSWAGLRPRAPDDLPVLGKSNETDGLFFATG
                     HYRNGILLAPLTGELIAALIADNVVAEELH"
BASE COUNT          482 a          618 c          619 g          505 t
ORIGIN      
        1 gctgtcttgg aagtaccgcc aatgtcgcga gtacgaactt ttccctctgt aaacacttgc
       61 agcattgcct tttcgatccg gtccgccgcc tctcgttcat ccaggtaacg caacatcagg
      121 attcccgatt gcagcagcgc cgtgggattg gcaagcccct gtccggcaat gtccggcgcc
      181 gagccgtgca cagcttcgaa cacggagccg cgttcaccaa tgttcgcagc gggaaccaaa
      241 ccaagaccgc caatcaatcc tgcgcaaaga tctgaaacga tgtcgccata gagattctcc
      301 agcaacatga cgtcaaactg ctcgggccgc atgaccaatt gcatacaggc gttatcaatg
      361 atcttgtcgt ctgcaatgat ctcaggaaaa ccttccgcca ctttgcgaaa acaatcgaga
      421 aacagtccgt cagacagttt catgatgttc gccttatgca ccgccgtgat cttctttcgg
      481 ccttcgcgtt gcgagtattc aaacgcaaac ttcgcaatgc gcgtcgaagc tttctcggta
      541 atgatcttga tgctctcaac cacccctggc acaacgacgt gttcgatccc ggcgtagaga
      601 ctctcagtat tctctcgcac aatcacgaga tcgagttcgg gatagcgaca ggggatgttt
      661 ggaagggcgc gcaccgggcg caggttggca taaaggtcga gcgtcttgcg cagtcccaca
      721 ttgacggagg caaagccttt tccgactgga gttgtgatcg ggcctttgag cgcgaccttg
      781 ttgcgggtga tcgagtcgag cagttcattg gggagcgtcg tgccaaattt ctccaacgct
      841 tgagcgccgg cgtagtgagt ttcccagact acttccacgc ccgcggcctc gattatcctg
      901 acaacattag aagcgacttc cgggccgatg ccgtcgcccg gaatcagtgt gatagtatgt
      961 ttcataacga attgtcggct atttgctcaa tcttagtttc gaacacgcag ctccgcggta
     1021 atcgactccc tgccacgtgc gtcttggatc attcaacaga atagagccac aagtgtgaac
     1081 tgacaactga ctggaaacca aactcaaaaa ttgggaagtg caggatttaa tttggacgag
     1141 cgagctgacg tagtcgttat cggcggcggc gtggttggtt tgtcagtggc ccgctcgctg
     1201 gcgcttaagc aagttggcag cgtgttgcta ctggagcgtg gacagctcgg cagggaagcg
     1261 tcctttgcgg cggcgggcat gctcgcgcca caagcggaag caaatactga cgatgctttc
     1321 tttcaactgg cttgccggag ccgagatctt tatcaagctt ttgcggcgca gctgctcgaa
     1381 gaaaccggca tcgatgtcga gctcgatcgc accggcacgc tctactgtgc cttcaccgag
     1441 cacgactgca cggaaattgc cgcccgtttt gcgtggcagc gtagtcgtgg cctgccagtc
     1501 gaacaactca ctgccggcga ggcccgagaa cttgaaccga accttgcgcc taatctctta
     1561 cgtgcgttga agtttcctct cgacactcag gttgagaacc gcaggctgct cagtgccttg
     1621 attgcggcta acgaaaaact cggtgtgaaa tcacgcgtca acaccaccgt cgaatcgctg
     1681 cgaattgaaa gaggccaggt ggcaggtgtc ataacttcgc agggatttct caacacgcgc
     1741 aacgttgtca tcgcgagtgg cgcgtggacc tccttcatta gaattgaaag cgctgcgccg
     1801 cgaatacgca tcgaaccggt gcgcggtcag atgttgtgct tcgacgctaa gccagccctc
     1861 gcgcgccacg tgatttatag cccgagaggt tacctggttc cgcgtcgcga tggccgattg
     1921 ttagccggtt cgaccacgga gaaggccggt tttgcgaagg aagtaaccgc gaaggggctg
     1981 gaatcaatac gaacacgggc gctggaaatt gtgccgggcg ttgccggatt gacgctcgtt
     2041 gattcctggg ccggcttgcg gccgcgcgcc cccgatgatt tgcccgtttt aggtaagtcc
     2101 aatgaaactg acgggctgtt ttttgcgacc ggccattacc gcaacggcat cttactggct
     2161 ccgcttactg gcgagttgat tgccgcacta atagcggata atgtcgttgc agaggagctt
     2221 caca
//
LOCUS       DASUEX010000466         3541 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1579891, whole genome shotgun sequence.
ACCESSION   DASUEX010000466 DASUEX010000000
VERSION     DASUEX010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3541
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1579891"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1022)
                     /locus_tag="VFV61_07860"
     CDS             complement(<1..1022)
                     /locus_tag="VFV61_07860"
                     /inference="COORDINATES: protein motif:HMM:NF021466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="App1 family protein"
                     /protein_id="HEX4900433.1"
                     /translation="MKSAAPPRLPAGTGEEVTFFPSYGYREGNGWNIHVRSWAHQNRS
                     HVDRAITKLATVLATAKNKCDVTEMANFQARANDFEDRDKSFEKVIIKFDEDPEGKSY
                     EFNKRSDANGIAELDLTLTDERAKQLLASQRSENGWLTFSAISEDHTGRGRIRLIEPE
                     GVSLISDIDDTIKITNIPAGKDTVLKNTFCREFRALPEMAKSYSDLVGVPVHYVSGGP
                     QQMFGPLYDFLIVEPGGFPEGTFHLNFFPHNILSTETIRLAVGGFTSTFDHKVDEITK
                     IMKKFPRRQFILVGDSGEVDPEVYKKIRSERPDQVKEIRIRDLINDSDRNANPYRLEG
                     MVIIKT"
     gene            complement(1504..2868)
                     /gene="dnaB"
                     /locus_tag="VFV61_07865"
     CDS             complement(1504..2868)
                     /gene="dnaB"
                     /locus_tag="VFV61_07865"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF004384.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEX4900434.1"
                     /translation="MAVAEQSLERALPNSAEAERAILGGIVLDNSLISQAIELIKPED
                     LYVPSHRRIFMAMIALFERSAEINPILIGEELKKENALESVGGISFIANLTYGLPHST
                     NITHYAKVVRGKSMLRQLIRAAGKITQEALEQEDEPEIILDHAEHAIFQLADERIRQG
                     FVHVKPVAEELLEKIQEMEGRAAVLTGLTTGFADLDKMTSGLQKQDLIIVAAPPSMGK
                     TSLALMLAENAAIHAGAVVGIFSLEMSKEALVMRMLCSQGNIDAQRFRNGFLSRPEWA
                     QIAKSLGTLADAKIFLDDTAGISVLEMRAKARRLAAEQKRLDLIVVDYLQLMSGSAKR
                     VESRQQEVSQISRELKGLAKEMNVPVVALSQLSRAPESRSDHRPQLADLRESGALEQD
                     ADVVAFIYREEQYKTPEERANLPEDQKNVAELIVAKQRNGPTGTVDLRFTPASMRFDN
                     LYRE"
     gene            complement(3175..>3541)
                     /gene="rplI"
                     /locus_tag="VFV61_07870"
     CDS             complement(3175..>3541)
                     /gene="rplI"
                     /locus_tag="VFV61_07870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEX4900435.1"
                     /translation="TERASADAQAEQMRSLVLAFERKAGEQGALYGSVTSMDIAEALQ
                     QRGYEVDRHRLHLREPLKRLGDFTVPLRLHRDVTVDLQVRIAPEGELIVGHMTPEQEA
                     EMSKQSESAKADESAEAAE"
BASE COUNT          800 a          963 c          880 g          898 t
ORIGIN      
        1 tctgtcttga tgatcaccat tccctccagt cgatagggat tggcgttccg gtcggaatcg
       61 ttgatgagat cgcgaatccg gatttccttc acctgatcag gtctttcgct tctaatcttt
      121 ttgtaaactt ccgggtccac ctcgcccgag tctccaacca gaatgaattg ccggcgcgga
      181 aacttcttca tgatcttcgt aatctcgtct accttgtggt caaaggtaga ggtaaaccct
      241 cctacggcaa gcctaatggt ctctgttgac aagatattat gggggaaaaa gttcaggtga
      301 aaggttcctt ccggaaaacc tcccggttca acaatcagaa aatcatagag cggcccaaac
      361 atctgctgcg gtcctcccga aacatagtgc acgggaacac caaccagatc actgtagctc
      421 ttcgccattt ccggcaacgc cctaaattcc ctgcagaaag tgtttttcag caccgtgtct
      481 ttaccggccg gaatgttcgt aatcttgatt gtgtcgtcaa tatccgagat cagcgacact
      541 ccctcaggtt caatcaatcg gattcgaccg cggccggtat gatcctccga gatggcgcta
      601 aaagttaacc acccattttc tgatctctga ctcgccagca attgctttgc tcgctcgtcc
      661 gttaaagtta aatccagttc ggcaatccca ttggcatcac tgcgcttatt aaactcgtat
      721 gattttccct ccgggtcttc atcgaattta atgatcactt tctcgaacga tttgtcacga
      781 tcttcaaaat catttgcacg tgcttgaaag ttggccatct ctgtcacatc gcatttgttt
      841 ttcgcggtcg cgagcacagt cgccagttta gtgatggccc tatccacatg agacctgttc
      901 tgatgcgccc agctacgcac gtgaatattc catccgtttc cctctctgta gccatagctc
      961 gggaaaaagg tgacttcctc ccccgtacct gccggcagcc tggggggcgc ggcacttttc
     1021 aactcgttta cgagcgcggt actgctcttc ccaggttctg gagctttagt gcaccaactg
     1081 gaaagtgcag cgaaaagaat tgttaagagc aacaggcagt tgtggggttt catgtttacc
     1141 tcctctgtcg gtataacgaa acggattgag atctattctg cactgcccct gaaagagatg
     1201 tagacaagca tctgtcgctg acggtctatc cgcgccaatt gatcactcgg cagaagtcca
     1261 atgaagctgc tgaagaagtc tttgctgcga ggttagagtg agtttaccgc ataacgggac
     1321 agccaggatt atcgaagccc cttgacgcgc ggattattcc acctgcatgt ctgagggtca
     1381 agagttgatc accttgggta gcagctcagt ttcttttcat acttcagttg aagactgggt
     1441 tctgagaggc ctgtccattt gagctgccac acaaccgatc cgattgaacg cgaacactag
     1501 aatttattca cgataaaggt tatcaaaacg catactcgct ggtgtgaacc gcagatcgac
     1561 agtgccagtc gggccattac gctgcttagc aacaatcagt tcggcgacgt tcttttgatc
     1621 ttccggaaga ttagcgcgct cttcgggagt cttgtattgc tcttcgcggt aaatgaaggc
     1681 aacgacatcg gcgtcttgct cgagcgcacc cgactcacgc aggtcagcga gttgcggacg
     1741 atgatccgaa cgcgactccg gcgcgcgcga cagttgcgac agcgccacca ccggaacatt
     1801 catctccttc gccagtcctt ttagctcacg cgaaatctgc gagacttcct gctggcgaga
     1861 ctctacccgc ttggcggaac ctgacataag ttgcaaatag tcaacaacaa tcaagtcgag
     1921 ccgtttttgt tccgccgcca gacgtcgagc tttcgcccgc atctcgagaa cgctgatgcc
     1981 ggcagtgtcg tcaagaaata tctttgcgtc ggcgagagtg ccgagcgact tggcaatttg
     2041 ggcccattcg ggtcgcgaaa gaaaaccgtt gcgaaaccgc tgtgcatcga tgttgccctg
     2101 cgaacaaagc atacgcatga ccagggcttc tttcgacatt tccaatgaga agatgccgac
     2161 caccgcgccc gcgtgaatgg cggcgttctc cgcaagcatc aaggctagac tggtctttcc
     2221 catcgaaggg ggggcggcaa cgatgatcaa atcctgtttc tgtaaaccgg aagtcatctt
     2281 gtcgagatca gcaaaacccg tagtcaatcc tgtcaggacg gccgcacgcc cttccatctc
     2341 ctggatcttt tctaaaagct cttccgccac cggcttcaca tgcacgaatc cctggcgaat
     2401 gcgttcgtca gcgagttgga aaattgcgtg ttcagcgtga tcgaggatga tctcgggttc
     2461 atcttcctgt tccagcgctt cctgggtgat tttcccggca gcacgaatga gctgtcgcag
     2521 catcgacttg ccccgcacca ccttcgcgta atgcgtaata ttggttgaat gcggcaagcc
     2581 gtaggtgaga ttggcgatga acgaaatgcc gccgactgac tcgagggcgt tctccttttt
     2641 cagttcctcg ccgatcagaa taggattgat ttctgcactg cgctcaaaca acgcaatcat
     2701 cgccatgaag atgcggcggt gcgatggcac ataaagatct tcgggcttaa ttagctcgat
     2761 tgcctggcta atcaaactgt tgtcgaggac gatgccgccg aggattgctc gttcagcctc
     2821 ggcactgttt ggtaaagcgc gttcgagtga ttgctctgcg actgccatga atatttagaa
     2881 gcgaatgttt gggtatgctg aaaaacggat gacgaacagt tgaccggcaa cgtcaggaat
     2941 atactacagg gctcgcaggt gaccaaaaat acggttgtgt tattagtatc gagccttgat
     3001 tcgtggcttt tacttatttc gtggttggtt cttaacggat tacttcaccg caaaaccaac
     3061 tactaaatac acgaacggcc actaataaga tcaattctgt tcccggtgtt ctgcctcaaa
     3121 gacaaagggc ggccaacaaa cggccgccca atgagctgat tggttagagt ttatctactc
     3181 tgccgcttcg gcgctctcgt ctgcctttgc gctttcgctt tgcttggaca tttcagcttc
     3241 ctgctctggc gtcatatgac cgacgatcag ttcgccttcc ggagcgattc gcacttgcaa
     3301 gtcgaccgtt acgtcgcgat gcaaacggag gggcacggtg aagtcgccca gacgttttaa
     3361 cggttcgcgt aagtgtaagc ggtgacgatc aacctcgtaa ccgcgctgct gcagggcttc
     3421 agcaatgtcc atcgaagtca cggagccgta gagtgcgcct tgttccccag ctttgcgctc
     3481 gaacgccagc accagactgc gcatttgctc ggcctgagca tccgccgagg cccgctccgt
     3541 c
//
LOCUS       DASUEX010000467         4115 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1217128, whole genome shotgun sequence.
ACCESSION   DASUEX010000467 DASUEX010000000
VERSION     DASUEX010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4115
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1217128"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2040)
                     /locus_tag="VFV61_07875"
     CDS             complement(<1..2040)
                     /locus_tag="VFV61_07875"
                     /inference="COORDINATES: protein motif:HMM:NF045064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900436.1"
                     /translation="MARQIWLGPLLGSNRARLIERCAALVTENKTNRFVYLAASHPLL
                     ELVTQGVLDGVRNRGVWNELPVYLFRGFVRRLLTSAANESGEKLPPQLPIDQDEFPLK
                     RSLVSQILIRLAAEGKLKAIGPLARREGCVNTITNLIGEIERAGKTPANLAAIVAERL
                     ADLGPQNELANSRRQIDFDKEVGLIYSTYAELLTKNSLTEADADQLRALALLNDESEF
                     QLSWLDNVDLLVLDGFFDFTPVQGEILRRLIPRIPEVLVNLNHDERNPEIFRPFNETI
                     NQLASIATFEIKSSSESFPATGALLPLRERLFNPALANQQPIEENESLAAGQTQNEIR
                     YFDCADRETEVRSIAKEIKRLVLVEDFVLADIALVVRERAAYAETIARVMHEESLPCN
                     LDRRVTVDQIPATRAALKLLGILEELCRNDASVLKMPQLADQIKSGYFRLSPEELETL
                     ALEFDQQFTALMNDGEAISEARDERRRRAFGIGRWDADTFENVIAYVGAELRLPDWLD
                     RCRKLITNLPGAEATKELLHIDTPEPTSDDEDALIEDAETVQPEDKHLEKKRRPQRDV
                     HPAVIAWSALVVKRFAALIQAIPREGTPADLRVAIMKLLEQFHFSRQVSRPIRHSEDD
                     ELPRPTLDLQALEGLRRGLLTAIKSIQLTQAETTSIPFVSLASVIDEVWRCLSAQT"
     gene            complement(2058..3521)
                     /gene="rmuC"
                     /locus_tag="VFV61_07880"
     CDS             complement(2058..3521)
                     /gene="rmuC"
                     /locus_tag="VFV61_07880"
                     /inference="COORDINATES: protein motif:HMM:NF014680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEX4900437.1"
                     /translation="MDNQILSVIIFLAGAVLGVFAGWFFLRTKASSATAADLATLKAR
                     LDGKDAELQRLQLALNSEVAEHKHSREESGQLKAALEGERRAAQERVAAFKNATDELS
                     EKFKALSRDALKDNNQSFLELAHSTLQKFQQGAKGDLELKQRAIDELVKPLKDSLEKV
                     DSKIGEMEKARVGAYSELREQVKALATSHLQLQQETGNLVTALRTPHIRGRWGEIQLR
                     RVVELAGMLQYCDFVEQETVVTADGRIRPDVIVRLPGNRTIVIDAKVPFDAFYESIAT
                     KDDLIRVTKLKDHARLVRTHIGSLSKKSYWESVQPTPEFVLLFLPGETFYSAALEMDP
                     KLIEDGVGQGVIIATPTTLIALLKAVSYGWRQEQMATNAQEVSKLGKELYDRLRTFTN
                     HFGDIGRGLDRALDAYNRGVRSLEARVLVTARKFKERGAIAGEEIATTESIDKVASPL
                     SLDEGGLFPEALAEVEPEEMVEDAPLFSLTATGGDER"
     gene            complement(3684..>4115)
                     /locus_tag="VFV61_07885"
     CDS             complement(3684..>4115)
                     /locus_tag="VFV61_07885"
                     /inference="COORDINATES: protein motif:HMM:NF021613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HEX4900438.1"
                     /translation="DAAGNEEHERRALTGFLHLLKDFDPESDEIVGHNILGFDLPYIF
                     QRCLVNEIPVRPFIDLSEFRVRGVFDTMHQWWLGGKRFVSLDDIAWALGIASSKTAEA
                     EGSKVFEMYAADKLAEIREYNLNDVRVTRQVYERMVACFGR"
BASE COUNT          895 a         1138 c         1109 g          973 t
ORIGIN      
        1 cgtttgcgca cttaaacagc gccacacttc atcgatcacg ctggccaagg atacgaaggg
       61 aatggacgtg gtttcagctt gggtaagctg gatgcttttg atcgctgtca acaaaccccg
      121 tcgcaaacct tcaagcgctt gcagatcgag agttggtcga ggcaactcgt catcttctga
      181 atgtcgaatg ggtctgctca cctgccgact gaagtgaaac tgctccagca gcttcatgat
      241 cgcgacccgc aagtcagccg gcgttccttc ccgtggtatc gcctggatca gcgcggcgaa
      301 gcgtttcact acgagggctg accacgcgat caccgcggga tgcacgtcgc gttgcgggcg
      361 gcgcttcttc tcgagatgct tatcctcggg ttgcaccgtc tccgcgtctt cgatgagcgc
      421 gtcttcatca tcactggtgg gttcgggcgt atcgatgtgc aaaagctctt tggttgcttc
      481 agcgccgggc aggttagtga ttagcttgcg gcagcggtcc aaccagtcgg gcaaacggag
      541 ctcggcgccc acataagcga tgacattttc aaacgtgtca gcgtcccagc ggccgatacc
      601 aaacgcgcga cgacgacgct catcacgggc ctcgctgatg gcctcgccgt cattcattaa
      661 agctgtaaac tgttgatcga actccagggc gagcgtttcg agctcttccg gtgaaagtcg
      721 aaaataacca gacttgattt ggtcggcgag ctgcggcatt ttcagcacgc tcgcatcatt
      781 acgacagagc tcctcaagaa tgccgagcaa cttaagagcg gcgcgcgttg ctggaatttg
      841 atcgacagtg acgcgacgat cgaggttgca gggtaacgac tcttcgtgca tcactcgcgc
      901 gattgtttct gcatatgcgg ctcgctcgcg aaccaccaac gcaatgtccg cgagcacaaa
      961 gtcttcaacc aatacgagcc gcttaatctc cttagcgatg gaccgaactt cagtttcgcg
     1021 gtcggcgcaa tcgaagtaac gaatttcgtt ttgcgtttgg ccggccgcta gggactcgtt
     1081 ttcctcaatt ggctgctggt ttgctagtgc cggattgaag agccgctctc taagtggcaa
     1141 cagagcgccc gtagccggga acgattccga gctggacttg atttcgaagg tagctattga
     1201 cgcaagctgg ttaatggttt cgttgaaagg cctaaagatc tccgggttac gctcatcgtg
     1261 attaagattc acgagcactt ccgggattcg ggggatcaag cgacgcagga tttctccctg
     1321 taccggcgtg aaatcgaaaa agccatccag tacgagcaaa tcgacgttgt ctaaccagga
     1381 aagttgaaac tcggactcgt cgttgagcag agcgagagcg cgtaactgat ctgcatcggc
     1441 ttcggtgagg ctgtttttgg tcagcagttc cgcataggtc gagtagatca agccgacttc
     1501 tttgtcgaaa tcaatctgac ggcgagagtt tgccagttca ttttgcggtc ctaagtcggc
     1561 gagccgctcg gcgacaatcg ccgccaggtt ggcaggtgtc ttcccggcgc gctcgatctc
     1621 gccgatcaga ttcgtgatgg tgttgacgca accctcgcga cgagcaagag gaccgattgc
     1681 ttttagtttt ccttcggcgg cgagccggat cagaatttgt gaaacaaggc tgcgcttgag
     1741 agggaactca tcctggtcta tgggaagctg tggtggcagc ttttcaccac tctcattcgc
     1801 cgcgctggtg agaagtcttc taacaaatcc gcgaaagagg taaacgggaa gttcattcca
     1861 aacgccgcga ttgcgaacac cgtccaggac accttgcgta acaagttcca ggagcggatg
     1921 ggatgcggcg aggtagacaa agcggttggt tttgttttcc gttacgagcg cagcgcagcg
     1981 ttcgatcaaa cgcgcgcgat tgctgcccag cagcggacct aaccaaatct gtcttgccaa
     2041 tttgactcca gacttcccta tcgttcatcg ccgccagttg cggtgaggct gaacaaagga
     2101 gcgtcttcga ccatttcttc cggctcaact tcagccagcg cctcaggaaa cagaccacct
     2161 tcatccaaac tcaacgggct tgccactttg tcgatggact cggtagtcgc gatttcttcg
     2221 ccggcgattg cgccccgctc cttaaacttt cgagcggtga caagcactct cgcttcgagt
     2281 gagcgcacgc cgcgattgta agcgtcgagg gcgcggtcaa ggccgcggcc gatgtcgccg
     2341 aagtgattgg tgaaagtgcg caggcgatca tagagctctt tccccaactt gcttacttcc
     2401 tgggcgttcg ttgccatttg ttcctgccgc cagccgtaag agaccgcttt cagcagggcg
     2461 atcagtgttg tcggcgtagc gataatcact ccctgtccca cgccgtcttc gatcagcttg
     2521 ggatccattt ccagggcggc gctgtaaaaa gtctcgcccg gtaagaagag cagaacaaat
     2581 tctggcgtcg gttgtactga ttcccaataa gacttcttgc tcagagatcc aatgtgagtg
     2641 cgtacgagtc gcgcgtggtc tttcaatttc gtgacccgaa tcaagtcgtc tttagtggca
     2701 atcgattcgt aaaaggcatc gaagggaact ttggcgtcga tgacgatcgt gcggttgccc
     2761 ggcaggcgta cgatcacatc gggccgaatg cgaccgtctg cagtaacaac tgtttcctgt
     2821 tccacaaaat cgcaatactg cagcatgcca gccaactcca cgactcgtcg caactgaatt
     2881 tcgccccagc gaccgcgaat gtgtggggtg cgcaaggcag tgaccaggtt gccggtttcc
     2941 tgctggagct ggaggtggga agtggcgagt gctttcactt gttcacgtag ctcagaatag
     3001 gcaccgactc gagccttctc catctctccg atcttggaat caaccttttc cagcgagtct
     3061 ttcaacggct taaccagctc atcgattgcc ctttgcttga gctctaagtc tcctttggcg
     3121 ccctgttgaa atttttgcag cgtcgagtgg gccagctcga gaaacgactg gttgttatct
     3181 ttcaatgcgt cgcgcgaaag cgctttgaac ttctccgaaa gttcgtcagt cgcattcttg
     3241 aatgccgcca ctctttcctg tgctgccctg cgctcacctt ccaaagctgc tttcagttgc
     3301 ccgctctctt cgcgggaatg cttgtgttcc gctacctcgc tgtttagcgc taactgcagt
     3361 cgctgcagtt cagcatcttt gccgtcgagt cgtgccttaa gggtggccaa atcagcagca
     3421 gtggccgaac tggctttagt tcgcaggaag aaccacccgg cgaagacacc caagaccgcg
     3481 ccggccaaga aaataattac tgagaggatt tgattgtcca ttgctgaatt gaaactaagg
     3541 ggagtctagc aaatttgttg caaacacgaa acaccggccc gttaacgggc cgttttattt
     3601 gggcggggag attcaaggtt tcaggtttca ggtttgcgag cgtaacgggt ccggaaacct
     3661 gaaaccttga acctgaaacc tacctatcta ccaaaacacg ccaccatgcg ctcataaacc
     3721 tgacgcgtaa ctcgtacgtc gttcagattg tattcgcgga tctcggcgag cttgtccgcg
     3781 gcatacatct cgaaaacctt gctgccttca gcttccgccg tcttgctcga cgcaatcccc
     3841 aaggcccagg caatatcgtc gagacttaca aagcgcttcc cgccgagcca ccactgatgc
     3901 atcgtgtcaa acactcccct gacacgaaac tccgacaagt cgatgaaggg gcgcactgga
     3961 atctcgttta ccagacagcg ttgaaaaatg tacggcagat cgaaaccaag gatgttatgg
     4021 cccacaatct cgtcactctc aggatcgaaa tctttaagta ggtgcagaaa gccagtcaaa
     4081 gcgcggcgct catgttcctc gttgcctgcg gcatc
//
LOCUS       DASUEX010000468         2177 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1667612, whole genome shotgun sequence.
ACCESSION   DASUEX010000468 DASUEX010000000
VERSION     DASUEX010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1667612"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="VFV61_07890"
     CDS             <1..348
                     /locus_tag="VFV61_07890"
                     /inference="COORDINATES: protein motif:HMM:NF019561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HEX4900439.1"
                     /translation="LEGQPAAGFELVSSTAQPSVINVNGPASLIEKLQRAPTELISIE
                     GKKGNFTALGVTIAIADSRIEVENPVVDVAIEIGEKKSPATTPLSSTSIFGDHLIAAK
                     TRTATAQPSQPIQ"
     gene            377..1732
                     /gene="glmM"
                     /locus_tag="VFV61_07895"
     CDS             377..1732
                     /gene="glmM"
                     /locus_tag="VFV61_07895"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEX4900440.1"
                     /translation="MTTPIKRRLFGTDGVRGEAGRFPLDAATMETIGASLAVRLGQSP
                     LIVVGRDTRESGDWLERALIAGALRRGARCESAGVITTPGVAFLTRSLGAQAGVVISA
                     SHNPYQDNGVKIFAPSGRKLDDDTERLIEADIFATQDSPATKSQPALTAADHSAELCA
                     RYLTFLEKEIGGGLSLRDQVIVVDAANGAASQLAPELFSRLGARVIAINNKPDGRNIN
                     LNCGSLHIEGLQQQVVVSGATLGVAFDGDADRALFVDAHGEFVDGDATLWVLANSLQM
                     RGELKQNLVVATVMSNIGLEIALRSRNLKLLRTDVGDKYVLEELLRTGASLGGEQSGH
                     IIFPEISLAGDGMITTLCLLRAMTHEQQALHQLTQGFQRFPQILVNIKVREKRPFTDV
                     PAIMDLANKTEARLGHEGRLLLRYSGTESLARVMIEGKNQSDIERYAGDLAAVIREQL
                     G"
BASE COUNT          497 a          593 c          613 g          474 t
ORIGIN      
        1 ctcgaaggtc agcccgccgc cggctttgaa cttgttagtt caactgcgca accgagtgtg
       61 atcaatgtta acggccctgc gagtctgatt gagaaattgc aacgagcacc caccgaactc
      121 atttcaatcg agggcaagaa aggtaacttc acagctctgg gcgtaacgat cgcgattgcc
      181 gactcgagga tcgaggtgga gaaccccgtc gtcgacgtgg caattgagat cggcgaaaag
      241 aagagccctg cgactacgcc gcttagttca acttccatct ttggcgatca tttgattgct
      301 gcgaaaaccc gaactgccac tgcccaaccc tcccaaccga ttcaataatg accccgcttc
      361 tgccacggcg ctgctcatga caactccaat taaacgaaga ctctttggta ctgatggtgt
      421 gcgcggggaa gcggggcgct ttccgctcga cgccgcaact atggaaacta tcggcgcctc
      481 actcgcagtt cgcctcgggc aatcacctct aatcgtggtg ggacgcgata cgcgcgaatc
      541 aggcgactgg cttgaaaggg cattaatcgc gggtgctctg cggcgcggcg ctcgttgtga
      601 atccgccggt gtcattacca caccgggtgt tgcgtttctc acccgcagtc ttggcgctca
      661 ggcaggcgta gtcattagtg cttcccacaa tccgtatcag gataatggcg tcaaaatatt
      721 cgcgccgagc gggcgaaaac tggatgatga caccgagcgt ttgatcgaag ccgatatttt
      781 cgcaacccag gattcaccgg caacaaagtc acaaccagca ttaacggctg cagatcacag
      841 cgccgaactg tgcgctcgct atctcacatt cctcgagaag gaaatcggcg gaggcctgtc
      901 gctgcgggat caagtcatcg tcgtcgatgc ggcaaatggc gccgcttcgc aattggcgcc
      961 ggagttgttc tcccgacttg gagcgcgcgt gatcgccatc aacaataaac ctgatggccg
     1021 gaacatcaat ctgaattgcg gctcgctgca catcgaaggc ttgcagcagc aggtggtggt
     1081 aagcggcgcc acgctgggtg ttgcgtttga tggtgacgcc gaccgcgcgc tcttcgtgga
     1141 tgcgcatgga gaattcgtag acggcgatgc aaccctctgg gtgttggcta acagtctcca
     1201 aatgcgcggc gagctcaaac aaaacctggt cgtggctacc gttatgagca acatcggact
     1261 ggaaatcgcc ttgcggtccc gcaatctgaa gttgctgcgc accgatgtgg gcgataagta
     1321 cgtactcgaa gaactactgc gcaccggtgc cagcctcggt ggagagcaat cgggtcacat
     1381 tatcttcccc gaaatcagct tggctggcga cggtatgatc accacgcttt gtttgcttcg
     1441 cgccatgacg cacgagcaac aggcgctgca ccagttaacg caagggtttc agcgttttcc
     1501 gcaaattctg gtcaacatca aggtacgaga gaaacgaccg tttaccgatg ttccggcgat
     1561 tatggacctg gcaaataaga cggaagcgag gctgggccat gagggccgcc tgctgctgcg
     1621 ttactcgggc accgagtcgt tggcgcgggt aatgatcgaa gggaaaaatc agagcgacat
     1681 cgaacgctat gctggtgatc tcgcagccgt aattcgggag caactgggct gagttttgcg
     1741 gctgatcaat gcaatttcta catgcagaac tcggaaagaa tttcactccc cgcagtcctg
     1801 gaagtcgcac tttgtcgttc agaaaacaac gcttgcgaag gaacttaaag gaaaggcgcg
     1861 agcgcgcgta aaacttttcc aatctgttgc acggcacagc cgttgcgatg tatattgtgg
     1921 ggcaagagag acggttggtg aatcctccgt ctcaagtggt tgctggggtg aggacagtga
     1981 ccacaaaaat ggatgggcgc gacgcactcc cacgtatgtc gcgcccatcc gctttttcgc
     2041 aagccttccg acttttttca ctgtattctt agtcgcttcg cgtgctgctt tcccacgacg
     2101 aattgagcta agtgcttagt gaacggagtc cagttagtgc acaccaagat cctgaaggta
     2161 agcgtccgtc ttgcccg
//
LOCUS       DASUEX010000469         4612 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_88435, whole genome shotgun sequence.
ACCESSION   DASUEX010000469 DASUEX010000000
VERSION     DASUEX010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4612
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88435"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(611..2152)
                     /locus_tag="VFV61_07900"
     CDS             complement(611..2152)
                     /locus_tag="VFV61_07900"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX4900441.1"
                     /translation="MRILLYNPDNGVTRNFMPHLWMFLLRALTPPEHEVLLIDGNAKP
                     LSESDLVQYVRDEKIDLVGIGAMTRMIAKAYRVADAVRAIGVPVVMGGPHVTELPDEA
                     LGRDGGPRHADAVALGEADETWPQILRDAEAGELRDVYEPVDETGKERKPGLQPYPVI
                     PWDTIDLDQFNLIPKFLRPLAERHGNGVRFFNTIPLESGRGCPYGCEFCTVTGFFGDS
                     IRFRTNQSVVDEMLLLKKRSQKERRQITIFFIDDNFAINIKRTKSLLRDIIAANAQLP
                     WIAQISANLLRDEELVDLIAESGGRWVFIGMESIDPGSLADVNKSFNKPSEYAAVLNR
                     LAKRNVYAITSFIFGLDNDTPGVAERTLEQIRQWPPVMPVFSPLIPLPGTPLYKRLGA
                     EGRLTRPKHWLNFAHNKMAHTPLKISIADAQKEIDKAWRVSYSPKSNDEVVDLLRDQR
                     FDDRMIHFVMRMFFRGIYFPQMNKRAWVKLIAQNRRPIMKLVKDGVGQYRAAKKRKAA
                     VEAAA"
     gene            2458..3684
                     /locus_tag="VFV61_07905"
     CDS             2458..3684
                     /locus_tag="VFV61_07905"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX4900442.1"
                     /translation="MPFFEAIKLALASILSHKLRSFLTLLGVIFGVATVIVVVSLIEG
                     FNAYVDEKIANIGTNAFSVSKYSIEDFSSVEALNAARRRNKDVTLEDAAALMSRRGAI
                     QDVGAKEDMAGDVKFGAVNLFRVHISATTANIAEIERIETGEGRYFTKIDELNRRNVT
                     FIGADVADKLFPAGNPLGQIIRIDGRPFEVIGVGKAKGSVFGQPQDMYVAMPLSTLLA
                     LYGSRRSLNLSVTSTSAATYQDAVDEARVVMRTRRKLSPNEKDNFGIITPSAINELRD
                     KIFGTIQTAAIGITSISLVVGGIVIMNIMLVSVTERTKEIGIRKSIGARRTDILKQFL
                     AESTLLSLVGGAIGISIAYLLAKLVAVLTPVPTVLPMLAVTVAFLVSGSVGLISGVYP
                     AWKAAKLDPIEALRAD"
     gene            3726..>4612
                     /locus_tag="VFV61_07910"
     CDS             3726..>4612
                     /locus_tag="VFV61_07910"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX4900443.1"
                     /translation="MRLFNSSIFENVRMGLDTLWHHKLRSLLTILGVVIGTMTVIVIA
                     AFVAGIDARVSKEIESFGTNSIYIYRFDPGFNFNPSQEERMRKPLSYEDTLAIADECP
                     SIAFAAAFMSPVDFFGGPFTQRVNVRYRDIEMTNAAVQGASPSYFKMGVTTIADGRYF
                     TDEENSRHANVLVIGNDVANTLFPFTNAVDREVTINGRSYRVVGVLAERDVFLVGAED
                     PNNENKAVYLPYLTLRKLYPEVKDNFVMAQARPGQMKEAVEEVRELLRRRRKVTFGDP
                     DNFGISTSEQILSQFGAITG"
BASE COUNT         1077 a         1286 c         1195 g         1054 t
ORIGIN      
        1 ccagatatcg ccgcggaagc ggccaagcgg cgattgatga gcggccgctg agccgcgttt
       61 tagacacgca ctggcgatcg gagttgctga gctgagatcg atttcttggt tcaggcaggc
      121 tttgcctcaa aagtcgaaaa cgctacatcc cgacgctagc gcgtcgcaat acctccctcc
      181 atcttttcca ctctcgcaac ccaccaggcc aaagtcgtca ccctcctcga cgacttgctg
      241 ccctcctgca cattccctcc aacaagaacc actaaagaca agcacacccg gaaccctctg
      301 gtcctcggtt gtgtaactgg ctgccacctg caacgtctgc gaggaagact cggcgggcga
      361 agatcagaac tgagtttgtg aggctgcgaa caccgaaact gaggacgtac tggtccaaac
      421 tagcggaaac agagggaaag tgacctttta agactgcgaa tcagggcgtc gcacgttcga
      481 gtcgtcccgg gtgcaccatt tcgcttgcgc tgctaagggc gttcaccctt ggacgccctt
      541 tttgcacgat catatgcagc caaaaagggc gccccgaggc gcccttcaag gtttttaact
      601 agatttggtt tcaggccgca gcttcgaccg cagccttgcg tttctttgcc gcgcgatact
      661 gaccgactcc gtccttcacc agcttcataa tcggccggcg gttttgtgca atcagtttca
      721 cccaggcacg cttgttcatt tgtggaaagt agatgccgcg gaaaaacatg cgcatgacga
      781 aatggatcat ccgatcgtca aaccgttgat cccgcaacaa gtcaactact tcatcgttgc
      841 tttttggact gtaggaaact cgccacgctt tatcgatctc cttctgtgca tccgcaattg
      901 aaattttcaa aggcgtgtgg gccatcttgt tgtgggcaaa gttcagccaa tgcttgggac
      961 gcgtcaaccg accttccgcc ccgaggcgct tgtacaaagg cgtgcccggc aacggaatca
     1021 gaggactgaa aaccggcatc accggcggcc actgccggat ctgttccagc gtgcgttcgg
     1081 cgacgccagg cgtgtcattg tccaatccga aaatgaaaga agtgatggcg taaacattcc
     1141 gctttgccaa gcggttgagc actgccgcgt attcgctggg cttattgaag ctcttgttca
     1201 catccgccag gcttccggga tcgatcgact ccatgccgat gaagacccaa cggccacctg
     1261 attcagcaat cagatcaacc aactcctcat cacgcaaaag gttcgcgctg atttgcgcaa
     1321 tccacggcaa ttgagcattc gcggcgataa tgtctctcaa caacgatttg gttcgcttga
     1381 tattaatggc gaaattatcg tcgataaaaa agatcgttat ctggcgacgc tccttttggg
     1441 aacgcttctt cagcaacagc atctcatcga cgacactctg gttggtgcgg aacctgatgg
     1501 agtcgccaaa aaatccggtt acggtgcaaa actcacaacc gtaaggacaa ccgcgacccg
     1561 actccagcgg gatggtgttg aagaatcgga cgccgttacc atggcgttca gccagtgggc
     1621 gcaaaaattt tggaatcaga ttgaattgat cgaggtcaat cgtatcccat ggaattacag
     1681 gatacggctg caagccgggc ttccgttcct ttcccgtctc atcaacaggc tcgtagacat
     1741 ctcgcaactc accggcttcc gcatctcgga ggatctgggg ccacgtctcg tccgcttcgc
     1801 ccagtgccac cgcatcagca tggcgcgggc cgccgtcacg gcccaatgct tcgtctggca
     1861 attcagtcac atgcggacca cccataacga ctggtactcc gatcgccctc actgcatcag
     1921 cgacccggta agccttggcg atcatcctgg tcattgctcc aatgccgacg agatctattt
     1981 tttcgtcgcg aacatattgg accagatcac tctcactcaa tggtttggcg tttccatcga
     2041 tcagcaatac ttcgtgctcc gggggcgtaa gcgctcgcag caagaacatc cacaggtgag
     2101 gcataaagtt gcgcgtaacg ccgttgtcag gattgtagag caaaatcctc atcttcccga
     2161 tttccccctt cggtagaata gaagtcgtta tgtggtgtgc taacgggtat tagctggcaa
     2221 gaagactcga tttttatacc cgcccagggt cgagaagtct gcttagtgcg ggctactata
     2281 ccaccgcttc cagcgaccgt atataccgga agcgtaacat agggggctgt gacccttgaa
     2341 acttctctcg agactccagc gtagatttaa agcacgttac catagcacaa caagtttaat
     2401 gccgccgggg cttgttactc ctgtatattc acaaatcaat cgcgatggtc ttgagaaatg
     2461 cccttctttg aagccataaa actcgccctc gcttcaatac tctcgcataa gctgaggtcc
     2521 tttctgactc tgctgggggt gattttcggt gtcgcaaccg tcatcgtggt ggtgtctttg
     2581 atcgaaggct tcaatgcata tgtcgatgaa aaaatcgcca acatcggaac aaacgccttt
     2641 tcggtcagca agtattcgat tgaagacttc tccagcgtag aggcgctgaa cgcggctcga
     2701 cgacgcaata aggacgtgac gctcgaagat gccgcggctc taatgtcgcg gagaggagcc
     2761 attcaagacg ttggcgccaa ggaagacatg gccggtgatg tgaaattcgg cgctgtaaat
     2821 ctctttcgcg tccacatcag cgccacaact gccaacatcg cagagatcga acgcatcgag
     2881 acgggtgagg gtaggtactt cacaaagatc gatgagctaa accgccgcaa cgtcaccttt
     2941 atcggcgccg atgtcgccga caaactcttc ccagcgggaa atccgttggg ccagattatt
     3001 agaattgatg gccgcccatt tgaggtgatc ggcgtgggca aagccaaggg ctctgtcttt
     3061 ggacagcctc aagacatgta cgtggccatg ccactatcga cccttctcgc actctacgga
     3121 tcccgccgct cactcaatct gagtgtcacc tcaaccagtg cggccacata tcaggacgct
     3181 gtcgatgagg cgcgcgtggt aatgcggacg cggcgaaaac tctctccgaa cgagaaggac
     3241 aactttggga tcatcacgcc aagcgccatc aatgagctac gcgacaagat tttcggcacc
     3301 attcagacgg cggcgatcgg catcacctca atctcgctgg tggtaggcgg catcgttatc
     3361 atgaatatca tgctcgtaag cgttactgag cgcactaagg agattggcat tcgcaaaagc
     3421 attggcgcac ggcgcaccga catcctgaag cagtttttgg cggaatcaac gctcttgtct
     3481 ttggttggag gtgcgatcgg gatctctatt gcttacttac tggccaagct ggtggcagtg
     3541 ctcaccccag tgccaactgt attgccgatg ctggcggtaa cggtggcgtt cctggtttcc
     3601 ggaagcgtag gcctgatttc cggcgtctat ccggcgtgga aggcggcgaa gctggatcca
     3661 attgaagcat tgcgcgcgga ttagtatttt tctcacgcga acaaagaacc aaaggctgaa
     3721 tatcgatgag gctcttcaac agtagcatct tcgagaacgt gcggatgggc ctggacaccc
     3781 tttggcacca caagctgcgc tcgctgctca ctatcctggg cgtcgtgatc ggaaccatga
     3841 cggtgatcgt aatagctgcc ttcgttgcgg gcatcgatgc tcgagtctca aaagagattg
     3901 agtcgttcgg tacaaactcc atctacatct accgcttcga ccccggtttc aacttcaatc
     3961 cttcacagga agaacgaatg cgaaagccgc tcagttatga agacactctc gcgatcgcag
     4021 atgaatgtcc atccattgcg tttgccgccg ccttcatgtc gcccgtggac ttctttggcg
     4081 ggccattcac gcagcgcgtt aacgtgcggt atcgcgacat cgagatgaca aacgcggcag
     4141 tacagggagc atcaccgtcc tacttcaaga tgggcgtcac cacgatcgcg gatgggcgtt
     4201 actttaccga cgaggaaaac agccgccatg cgaatgttct cgtgattgga aacgatgttg
     4261 ccaacacact tttccccttt accaatgccg tcgatcgcga ggtaacgatc aacggaagaa
     4321 gttatcgcgt tgtcggcgtg ctggccgaac gcgatgtctt cctcgttggc gccgaggatc
     4381 ccaacaacga aaacaaggcg gtctatctac cttatctaac gctgagaaag ctctatcccg
     4441 aagtgaaaga caatttcgtc atggcccagg ccagaccagg ccaaatgaag gaagcagtcg
     4501 aagaggtgcg cgagttgcta aggcgccggc gtaaggtgac ttttggtgat cctgacaatt
     4561 ttggtatctc cacttccgaa cagatccttt cgcagtttgg tgctatcaca gg
//
LOCUS       DASUEX010000470         3157 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_762820, whole genome shotgun sequence.
ACCESSION   DASUEX010000470 DASUEX010000000
VERSION     DASUEX010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3157
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_762820"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(169..1782)
                     /locus_tag="VFV61_07915"
     CDS             complement(169..1782)
                     /locus_tag="VFV61_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900444.1"
                     /translation="MRRFLLIVIALSVVGLGLPSFSSRAQNDSKPQKELKMSKPEVVQ
                     ATKHDQSRPLREIAPLAPKKDREYENPMNFVKKPVVDQPDGAVQSSAVPSVPVINGLN
                     FEGIGEGLVGYNVNVAPPDTTGDVGLTQYVQWVNLSFAVFDKNTGNMVYGPAAGNTIW
                     SGFGGPCATSNDGDPIVQYDQLANRWILTQFAVSTTPYRQCVAVSTTSDATGSYNRYE
                     FTYGTSFNDYPKLGVWPDGYYITYNMFANASSFSGSRVCALDRARMIAGLSATQQCFQ
                     LSSSFGSVLPADLDGPTAPPAGAPNYMITDGSNSLLFWRFHVDWTTPANTTLTGPITI
                     PVASFSHPCPTTSRGACVPQPGTSQKLETLADRLMYRFSYRNFGTHESLVVNQAVKVG
                     SNKRNTTTGIRWYELRNPGGSPPTVFQQSTYAPTADWRWMGSAAMDKLGNLAVGYSIS
                     NSSSLRPTIRFAARNISDPLSTLSSEVNLLTGTGSQTQSLARWGDYSTLSVDPSDDCT
                     MWYTNEYLTVSGTFNWHTRIASFKLGACQ"
     gene            complement(2038..>3157)
                     /locus_tag="VFV61_07920"
     CDS             complement(2038..>3157)
                     /locus_tag="VFV61_07920"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX4900445.1"
                     /translation="AEEVVMALMTGNSVVLKPSELTPLIALKIHEIFKRAGLPEDVLQ
                     VVTGDGSTGAALVDAGVDKLIFTGSVATGKRVAEAAARHLTPVVLELGGKDPMIVFAD
                     ADLENAARGAVWGAFANAGQACASVERCYVEESIAPKFLDLVVASTKALTQGAGTEAG
                     VDIGAISNEAQLQLIEKQVQDAVRQGATIVTGGKRLDGPEFKRGLFYQPTVVTNVNHS
                     MELMREETFGPVLPLMTFRSEAEALTLANDSVFGLTASVWTKDLRKGRRVAEKIEAGT
                     VMINEVLYTHGIAETPWGGVKQSGYGRTHGRLGLLEMVTPKHIHLNRITFLPDLWWFR
                     YDVKATKLFRGLAKQFTSGSPIQTARLLPQMIRRFFRS"
BASE COUNT          692 a          858 c          843 g          764 t
ORIGIN      
        1 aaaacttcag aatacctctt cagggcgtaa tgcgatcggg agtttgtaat cctaaaggtg
       61 gctggacagt agatcaagtg acggcgccaa tgccccaact gcgtcaatat ttagggccgt
      121 ccggttttac cgaacggccc aaaaagcgga gcatcccttg ttaacggttt attggcatgc
      181 gccgagcttg aaagaggcaa ttcgagtatg ccaattgaac gtgccactga ccgtcaggta
      241 ttcattcgtg taccacatag tgcagtcgtc actcgggtcg acactgagtg ttgaatagtc
      301 gccccagcga gccagactct gtgtttgtga accagtaccg gtgagtagat tcacttcgga
      361 actgagtgtg ctgagcggat cggaaatatt tctggccgca aaccgaatag ttggtctcag
      421 gctggaactg ttcgagatgc tgtagccgac cgcgaggttc cccagcttgt ccatcgctgc
      481 actccccatc caacgccagt cagcagttgg ggcataggtt gactgttgga agacggtcgg
      541 tggactgccg cctggatttc gcagctcgta ccagcgaata ccggtcgtcg tgttcctctt
      601 gttacttccg actttcaccg cttgattgac caccagtgat tcgtgtgtgc cgaagttgcg
      661 ataagagaat cgatacataa gtcgatcagc caatgtctcc agtttctggc ttgtgcccgg
      721 ttgcggcacg catgcaccgc gactggtggt aggacatggg tggctgaacg acgccacggg
      781 gatcgtgatc gggcctgtca gggtcgtgtt tgctggcgtg gtccagtcaa catgaaatct
      841 ccaaaacagc agagagtttg agccgtccgt aatcatgtag ttgggcgcac cagcaggagg
      901 cgctgtcggc ccgtccaaat cggccggcaa cacacttccg aaagagctgc tgagctgaaa
      961 acattgttgc gttgctgaca gacccgcaat cattctggcg cgatcaagag cacacactcg
     1021 cgatccgctg aacgaagatg cgttggcaaa catgttgtag gtgatgtagt aaccgtccgg
     1081 ccagacaccc aactttggat agtcattaaa gctggtgcca taggtgaact cgtagcggtt
     1141 gtaactgccg gtcgcatcag atgtggtgga cactgctacg cactgtcgat acggcgtggt
     1201 tgagaccgcg aactgcgtca agatccagcg attcgccaac tggtcatact gcactatcgg
     1261 gtcaccgtcg ttactggtgg cgcatggtcc accaaagccg ctccagatag tgtttccggc
     1321 tgctggtcca taaaccatgt tgccggtgtt cttgtcgaaa acggcaaaag agagattcac
     1381 ccattggaca tactgcgtta atcccacgtc gccggtcgtg tcaggtggcg ccacgtttac
     1441 gttgtagccc accaacccct cgccaattcc ctcaaagttc aagccattga taacggggac
     1501 gctgggaacc gcggacgatt gaacagcgcc atctggctgg tcaaccacgg gttttttgac
     1561 aaagttcatg gggttctcgt attcgcggtc tttcttgggt gccaggggag ctatttcacg
     1621 cagaggccgc gactgatcat gtttcgtcgc ctggacgact tccggcttgg acatcttgag
     1681 ttctttctga ggtttcgagt cgttttgggc ccttgaacta aatgaaggga gccctaaccc
     1741 gacaactgat agcgcaataa cgatcagtaa gaatcttcgc atttgtattc ctctctcata
     1801 tggctgggct ggttaagggc cagtcgggag tgagcatttg ggttattttg agttaatgct
     1861 tggatatttc tcgtgaggcg gaacccctat tccgaccgcc ggaaatacga accgctgtag
     1921 tagcagcgtg gcgcagtata gccctttctc gacgcaaagc aactggaatt ttgtaatgaa
     1981 ttacggtttt ctggacacgc gcaggcggac ggaagaaatc cagctttcag ctgcgattca
     2041 ggaacgaaag aagcggcgga tcatttgcgg gagcaggcgc gcggtttgga ttggcgagcc
     2101 gctggtaaat tgtttcgcta aacctcggaa taattttgtg gccttcacgt catagcgaaa
     2161 ccaccacagg tccggtagga acgtaattcg attcaaatga atgtgctttg gcgtaaccat
     2221 ttccagcaat cccaggcgcc catgggtccg gccataaccg ctctgcttga cgccacccca
     2281 gggtgtctca gcgatgccgt gcgtatagag aacttcattg atcattacgg tcccggcttc
     2341 gattttctca gctactcgac gacccttgcg taagtctttt gtccagacgc tggcggtcaa
     2401 cccaaaaaca ctgtcgtttg ccagcgtcaa cgcttctgct tcactacgaa atgtcatgag
     2461 cggcagcacc gggccaaagg tttcttcccg catcaattcc atcgagtgat ttacattggt
     2521 cacaacggtg ggctggtaaa acagtcccct tttgaactct ggaccgtcca aacgttttcc
     2581 cccagtaaca atggtggctc cctgccgcac cgcgtcttgc acctgtttct ctatcaactg
     2641 cagttgtgct tcgtttgaga ttgcgccgat gtcgacacct gcttcagtgc cggcgccctg
     2701 cgtcagtgct ttggttgaag cgacgaccaa atcgagaaac ttgggcgcga tcgactcttc
     2761 gacgtaacaa cgttcgactg atgcgcacgc ctggcccgca ttcgcgaatg caccccagac
     2821 ggcgccgcgg gcagcgttct ccagatccgc gtcggcaaac acaatcatcg gatctttacc
     2881 gccgagctcc agtaccactg gcgtcaaatg tcttgccgcc gcctccgcta cccgtttacc
     2941 agtggctaca ctcccggtaa agatcagttt gtcgacacct gcatcgacca gtgccgctcc
     3001 agttgatcca tcaccagtca caacttgcag cacatcctct ggcagtcctg cacgtttaaa
     3061 gatctcatgg atcttcagtg ctatcagcgg agtcagttcg ctcggcttga ggaccactga
     3121 attacctgtc atgagggcca tgacgacttc ctctgcc
//
LOCUS       DASUEX010000471         3126 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1218610, whole genome shotgun sequence.
ACCESSION   DASUEX010000471 DASUEX010000000
VERSION     DASUEX010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3126
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1218610"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..844
                     /locus_tag="VFV61_07925"
     CDS             <1..844
                     /locus_tag="VFV61_07925"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HEX4900446.1"
                     /translation="MDDGTFGICDVCHEPVESGRLIADPLTRLCIDHLSPPEQRALED
                     DLELAGRIQAGLLPPPSQKIDGWEIAYHYQPAGVVSGDYCDVISDGDQNVLFVLGDVS
                     GKGVAASMLMAHLQAMFRTLISVALPLEQMIERASRVFCESTLPNHYATLVCGKTNRS
                     GLVEVCNAGHLPPLLFQAGSVRQIAATGLPVGIFCNEHFTVNRMQLERGDTLLLYTDG
                     FSEALNGDGKEYGVERLAHLLREHHGLPPHALLDACMRDWLSFGDGRRTTDDVTLMAI
                     QRSH"
     gene            complement(813..1709)
                     /locus_tag="VFV61_07930"
     CDS             complement(813..1709)
                     /locus_tag="VFV61_07930"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX4900447.1"
                     /translation="MTKVEWNRRQFLTGLAGAPLLAISATAVYARFIEPYYYRIAEQD
                     VFVRDLSPAFENFRITQITDIHHSRIVGIDEVDRVISLAQSTNPDLIALTGDYTTTYR
                     RFIEPCAATLGNLNAPEGVWAVLGNHDHYTDPELTTRALERHHISVLDNANTVLKRGA
                     DTLQLAGIDDWSWARTDWPRVFHGIDQNQPTVLLSHQPAVLDLAQTQRVSLILSGHTH
                     GGQVTVPFIGAPARLATKDLKYAHGLFRRGETQLYVSSGTGVIGLPMRLGVRPEIAVL
                     RLRRELRTSRRQLSVTAESPST"
     gene            1824..1914
                     /locus_tag="VFV61_07935"
     tRNA            1824..1914
                     /locus_tag="VFV61_07935"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1858..1860,aa:Ser,seq:gct)
     gene            2006..>3126
                     /gene="gltX"
                     /locus_tag="VFV61_07940"
     CDS             2006..>3126
                     /gene="gltX"
                     /locus_tag="VFV61_07940"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEX4900448.1"
                     /translation="MSVRVRFAPSPTGYLHIGAARSALFNWLFARKLGGKFLLRIEDT
                     DLQRSTDESTRSIIEGLHWLGLNYDEELVFQSANAPKHRAAANKLVAAGKAYRDFTPK
                     QERDDATVKQGIAERARAHAVEGTDHRSNHYRELDASESENRANAGEPFAVRLKVPRE
                     GKTHFDDLVYGAQERNYSEIEDLVLLRSDGHPLYNLSVVIDDIEMGITHVIRGQDHVT
                     NTHKQILLYEALGIPPPKFAHLPLILAPNKGKLSKRKHGEVVSLTTYRDRGFVPAAFR
                     NFLALLGWSPSDTKEIMTLEELIKEFSLEGIGRANAIFNFHENDPRHWTDDKALWMNA
                     EYIRTMPLTELLPMVKAELRANKLWRDEYEDEDRDWLIRT"
BASE COUNT          733 a          833 c          860 g          700 t
ORIGIN      
        1 aatggacgac gggactttcg gtatctgtga cgtctgccat gaaccagtgg aaagcgggag
       61 gcttatcgcc gatccgctga cacgcctctg cattgatcac ttgtcgcccc cagaacagcg
      121 ggctttagaa gacgatctcg aattggcggg tcggatccag gccggtttgc tgcccccacc
      181 cagtcagaag atcgacggct gggagatcgc ttatcactac cagccggcag gtgtagtcag
      241 cggagactat tgtgatgtca tcagcgacgg cgatcagaat gtgttgttcg tcttgggaga
      301 cgtatcgggc aaaggcgtcg cagcatcgat gttgatggcc catctacagg caatgttcag
      361 aactttgatt tcagtggcct tgcctttaga gcaaatgatt gagcgtgcga gtcgggtgtt
      421 ctgtgaaagt acgcttccca atcattacgc aacacttgtt tgtggcaaaa ccaaccgatc
      481 cggattggtg gaagtctgca acgccggaca cttgccgcca ttgctgtttc aggcagggtc
      541 tgtccgccaa attgctgcca ccggactacc agtcggcatt ttttgcaacg aacacttcac
      601 cgtgaatcga atgcagttgg aacgaggcga caccttgttg ctttacaccg atggattttc
      661 agaagccttg aatggtgacg gcaaggaata cggcgttgag cgactggcgc accttttacg
      721 tgagcatcat ggattgccgc cgcacgctct gcttgatgct tgtatgcgcg actggctttc
      781 ttttggagac ggacgacgaa caacggacga cgtcacgttg atggcgattc agcggtcaca
      841 ctgagctgcc gccgggaagt tcgtagttcg cggcgcaacc ggagtacagc gatttcgggt
      901 ctgacgccca gcctcatggg caatccgatc acacctgttc cactggacac atatagctgc
      961 gtctcacccc ggcgaaagag tccgtgggca tacttcagat ctttcgtggc cagacgagca
     1021 ggggctccaa tgaagggaac cgtgacctgg ccgccatgcg tgtggccact caatatcaac
     1081 gacactcgct gcgtttgggc aagatccaaa accgcgggct gatgcgatag caaaaccgtg
     1141 ggctggttct gatcgattcc atgaaaaacc cggggccaat ctgttcgcgc ccatgaccaa
     1201 tcatctatgc ccgccagttg cagtgtgtcg gctcctcgct tcagcaccgt gttggcgttg
     1261 tcgagaacgg atatgtgatg ccgctccaga gcgcgagtag tcaactctgg atcagtgtag
     1321 tgatcgtggt tgccaagtac ggcccaaaca ccctcaggtg cgttcaagtt gccgagcgta
     1381 gcagcacatg gttcgatgaa gcgacgatat gtcgttgtgt agtcgccggt caaagcaatc
     1441 agatctgggt tagtagactg tgccagactg atcacgcgat caacttcatc aataccaaca
     1501 atgcggctat ggtgaatgtc ggttatttgg gtgatgcgga aattttcaaa cgctggagac
     1561 aggtcgcgaa caaatacatc ctgttcagcg atgcggtaat agtagggttc gatgaaacgc
     1621 gcgtaaaccg ccgttgctga tatcgccagc aacggggcac cggccaggcc cgttaagaat
     1681 tgtcgacgat tccactcgac tttggtcacc tggcgattct atacggctac gcgcctcaca
     1741 ggatagcgtt actacctctg acagcaccct tcttgacaat ccacgaccgc agactagaat
     1801 tggttttcgc ctcgctcagg cacggagatg tggccgagtg gctgaaggcg gcggtttgct
     1861 aaaccgttaa gggtcaaaag cccttcacag gttcgaatcc tgtcatctcc gccagaattg
     1921 aaacgatgaa cgcggagcgc ggaacgctga tcgaaagtgc tatcttcagt tcagcgttca
     1981 tcgttccgcg ttcattattt gcatcatgtc cgttcgcgtc cgtttcgctc catcacctac
     2041 cggttacctg catatcggcg cagcgcgctc ggcgctgttt aattggctct tcgcgcgcaa
     2101 gctaggcggc aagtttctgc tgcgcatcga agataccgat ctgcaacgct caactgacga
     2161 gtcgacccgg tcgattatcg aagggttgca ctggttggga ttgaactacg acgaagaact
     2221 tgtctttcag tccgccaatg cgcctaaaca tcgggcggct gccaacaagt tggttgcagc
     2281 aggcaaggct taccgcgact ttactcccaa acaagaacgc gatgacgcaa ccgtaaagca
     2341 aggcatagct gagcgcgcgc gggcgcatgc cgtcgaaggc actgatcacc gcagcaatca
     2401 ctatcgcgaa ctcgacgcaa gcgagtctga aaatagggct aacgctgggg agccatttgc
     2461 agtgaggctc aaggtgcccc gtgaaggtaa gacccacttt gacgatcttg tttatggagc
     2521 acaggaacgg aactactccg aaatcgaaga tctcgtgttg ctaagatctg acgggcaccc
     2581 cctgtacaac ctttcggtgg tcattgacga catcgaaatg ggaatcacgc atgtgattcg
     2641 cgggcaagac cacgttacga acacgcacaa gcagatcctg ctctacgaag ccctgggcat
     2701 accacctcca aagtttgccc acttgccatt aatcctggcg ccgaacaagg gcaaactctc
     2761 caaacgtaaa catggcgagg tggtttcact taccacatac cgtgaccggg gctttgtgcc
     2821 cgcggcattt cggaatttcc tggcgctcct gggttggtca ccctccgata caaaggagat
     2881 catgacgctt gaggagttaa ttaaggagtt ttcgctcgag ggaattggtc gagcgaacgc
     2941 aattttcaac ttccatgaaa atgacccgag acactggacc gacgacaaag cactgtggat
     3001 gaatgccgag tacattcgca caatgcctct aaccgagctt ttgccgatgg tgaaagcgga
     3061 actacgcgct aacaaacttt ggcgcgacga gtacgaagat gaagatcgtg actggttgat
     3121 tcgcac
//
LOCUS       DASUEX010000472         2005 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_763200, whole genome shotgun sequence.
ACCESSION   DASUEX010000472 DASUEX010000000
VERSION     DASUEX010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_763200"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..977)
                     /locus_tag="VFV61_07945"
     CDS             complement(<1..977)
                     /locus_tag="VFV61_07945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEX4900449.1"
                     /translation="MHRNLRTFLDLLTREKEIITIDTEVDPYLEAAEIHRRVIERGGP
                     ALLFKRIKGSKYPVVTNLFGTARRIELAFGPKPEALVRNFTKIAEALLPPRSAELWKQ
                     RSVARDLFRLGTRNTSQAPVAEVIDRPARLNELPVLTTWEEDGGPFITLPLVYTENPK
                     THKHNLGIYRLQVFDEQSTGLHWQIQKGGGFHYKEAEALNQSLPVTVFLGGPPALILA
                     AIAPLPEDVPELVLASLLAGEKLRMTKNPVKAGHRLAAEAEFALIGQAPPHERRIEGP
                     FGDHYGYYSLEHEYPVFHVDAVCHRRDAIYPATVVGKPRQEDFFIGDYL"
     gene            complement(1136..1711)
                     /locus_tag="VFV61_07950"
     CDS             complement(1136..1711)
                     /locus_tag="VFV61_07950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900450.1"
                     /translation="MGQASATDDAGDPREVGKRWARQLGHYDPVVRQEAAEALARLAA
                     VEQKKLVEGYHLQEKNKNVRLALGWALYRMGKIDMLFQIVRELDSGRHDQAVGYLSKV
                     DTPTLLHSYLKDEDKPARITTGLIEALAVIGDQESLALITPFRDSFFPGVAAAAEEAI
                     EKIEARLGQSEPAKPTRPRTVSSSEKIDPDL"
BASE COUNT          441 a          558 c          546 g          460 t
ORIGIN      
        1 tgtaagtaat cgcctatgaa gaagtcttcc tgacgcggct tgcccaccac cgtggcggga
       61 tagatcgcat cgcgacgatg acaaactgcg tccacgtgaa aaacgggata ctcatgctcc
      121 agagagtagt aaccgtaatg atctccgaat ggcccttcga tgcgacgctc atggggcggc
      181 gcctgcccaa tcagtgcgaa ctccgcttcc gccgcgagac gatgccccgc cttgactggg
      241 ttctttgtca tgcgcagctt ctctccggca agcaacgacg ccagcaccag ctcgggaaca
      301 tcttcaggta aaggagcaat ggcagcgaga attagtgctg ggggaccgcc gaggaagaca
      361 gtgacgggca gactctggtt gagcgcttcg gcttccttgt aatgaaagcc gccacccttt
      421 tgaatctgcc aatgcaaccc ggttgattgc tcatcgaaaa cctggagtcg ataaattccg
      481 aggttgtgct tatgggtctt cgggttctca gtgtaaacga gtgggagggt gataaacggg
      541 ccgccgtctt cctcccaggt cgtcaggacg ggcagttcgt tcaagcgcgc cggtcgatcg
      601 atcacctcag ctactggagc ctggctagtg ttgcgtgttc caagtctaaa aagatcgcga
      661 gctaccgagc gttgcttcca aagttcagcg ctgcgcggtg gcaagagagc ttccgcaatc
      721 ttcgtaaagt tgcgcacgag ggcttcgggc tttggcccaa acgccagttc gatgcgacgc
      781 gctgtgccga acaggttggt aacaacggga tatttgctgc ccttgattcg tttgaagagc
      841 agcgccggac caccccgctc gatgacgcgc cgatggatct cggctgcctc aaggtaagga
      901 tcaacctcgg tgtcaattgt aatgatctct ttttcgcgcg tgaggaggtc gaggaatgtg
      961 cgcaggtttc ggtgcatcca accagggagt ataacctaac cgtctggagt tagtacttgg
     1021 tgcttcgtac tttgaacttg gagcttcgca cctaacctag aaccctgctg cgttggatag
     1081 aattactcct cgagagtcat aacaaaacca aagtacaaag ctcaaagtac aaagttcata
     1141 aatcagggtc gattttctcc gagctactca cagtgcgggg acgcgttggt tttgcaggtt
     1201 cgctttgtcc caggcgcgct tcaatcttct caatggcttc ttcggccgcc gcagccactc
     1261 cggggaaaaa agaatcgcgg aagggtgtta tgagagcgag actctcctga tcgccaatca
     1321 ccgccaacgc ctctattagc ccggttgtga tccgtgctgg cttgtcttcg tccttgagat
     1381 aactatgaag gagtgtggga gtatcgacct tactgagata acccaccgct tgatcgtgac
     1441 ggccggaatc caactcgcgc acgatctgaa acagcatgtc aatcttgccc atgcggtaga
     1501 gcgcccaccc gagcgcgagc cgcacgttct tgtttttctc ctgaaggtga tagccctcca
     1561 ccagtttctt ctgctcgacg gcagcgagtc gcgccaaagc ctcggccgct tcctggcgca
     1621 ccaccgggtc gtagtgacca agttgccgcg cccagcgctt gccgacttct cgcggatcac
     1681 ccgcatcgtc ggttgcggat gcctgcccca aacaaagaga ggggatacaa agaagcgata
     1741 gaagaattag acatctcagc accgggtgta ggattcgtgt attcataggt tttcaatctg
     1801 ctgcaatgat cgctatgcct tttcattctc accgggcttc agcccggtgt ccattatcgc
     1861 cgcaccgcgc tgaagcgcga tggaatgaga actactttgc cgccgtcgcg cttctcttgc
     1921 gcaaggcagc agtattcaga tccagctctg ataacggaat cacttctaca ttcgcaaact
     1981 gtttccgtaa cggctccaga aagtc
//
LOCUS       DASUEX010000473         2389 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1460183, whole genome shotgun sequence.
ACCESSION   DASUEX010000473 DASUEX010000000
VERSION     DASUEX010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1460183"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            505..2145
                     /gene="lnt"
                     /locus_tag="VFV61_07955"
     CDS             505..2145
                     /gene="lnt"
                     /locus_tag="VFV61_07955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEX4900451.1"
                     /translation="MFGKLWLMQQTAVTSSSPERGTSERFFTRVKYQALDLPSVGAAL
                     LSSFLLILAFPDFDLWPLAWIALLPLLLAIARKPSGPRAFMLGWLSGTVFFYCSCYWL
                     TFSMIHYGGLPVWLAYLLLFPGALVVGVFPALFAFTLSLAISRYRQYALLLAPIFWVA
                     FEWARLGVTGQLWNALGYSQAYQPWLIQAANWGGVYAVSFLIVTINAAVAFLIINRSK
                     RSIIVAASGVIAVIVVVFLSPIHAEQETSVAGRVVALQPNVPMALIKSAEEMRELTAR
                     HITMSEEGLGRLPHDTTPVLVIWPESPMNFTYAGDAQFREFISSFAREHGTSLLFNSQ
                     EPAPNNGIYNSAVLINRDGSMVGQYDKIRLLPFGEYVPLPRWLPGANLITAIVGDFSP
                     GVNYTLLPAGEAKAGVFICIESAYPSIARRFTEDGAEVLINISNDGYLGRTAVLRQHL
                     ANAIFRATENRRPLLRVTNTGITAFITKNGKVVDATDVFEPAVRVWDVKHNSSDRTFY
                     TRHGDLFALSCAVVTLIVFTLALLARGKAQRSRISENA"
     gene            2142..2389
                     /gene="prfB"
                     /locus_tag="VFV61_07960"
                     /pseudo
     CDS             2142..2389
                     /gene="prfB"
                     /locus_tag="VFV61_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_319006089.1"
                     /note="recognizes the termination signals UGA and UAA
                     during protein translation a specificity which is
                     dependent on amino acid residues residing in loops of the
                     L-shaped tRNA-like molecule of RF2; in some organisms
                     control of PrfB protein levels is maintained through a +1
                     ribosomal frameshifting mechanism; this protein is similar
                     to release factor 1; frameshifted; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
BASE COUNT          512 a          670 c          631 g          576 t
ORIGIN      
        1 tttccagaaa aactttttct gtggcatacg aggtgctcta aggttcgtcg gcacgaacgg
       61 aagaagcagg aagttgaaac cgttcccccg ccgccatcac ccctctgagc gccgcctgcc
      121 gctgcgccag ccaccccccg agcaaccctt cagtgctccc tccgaaatcg gaacccagtc
      181 agaaatggct gggttctccc tcttttgggg cctaatagca aaaccaaaca atcaatcaag
      241 tttccgcaag cacacgagac ttccctgtcc tgcctctgga agaagtgacg gccacaggag
      301 caagcgacgg aatttgtcac ttggcgggtg acgttttctg tctattaggg ccccaaatcg
      361 ttgcttcttc gacggattcc ggctcgccca aagtgtcttg aagaatttat ttttgcgagt
      421 gctgaaatcc tgacacaacc agctctgagc aggggcggta ggcccactcg gacagaatgt
      481 tctcgcgccc cgcgtggttc tgtaatgttt ggtaaactct ggctgatgca gcagacagcc
      541 gtaacctcct caagtcccga gcgaggaacg agcgagaggt tttttactcg tgtgaagtac
      601 caggcactag acctgccctc cgtcggcgcc gcgctgctgt cttcattcct cttaatacta
      661 gccttccccg actttgatct ctggccgctc gcctggattg ctcttcttcc gctcctgctg
      721 gcaatcgccc gcaagccgtc tggtcccagg gcattcatgc ttggctggct cagcgggact
      781 gtcttttttt attgctcctg ttattggcta acgttctcga tgatccacta tggcggcctg
      841 ccggtgtggc ttgcgtatct gcttctcttc cccggagcac ttgtggttgg agtctttccc
      901 gcgctctttg cttttaccct ctcccttgcc atcagccggt accgccaata cgcactgctc
      961 ctggccccca tcttctgggt tgcttttgaa tgggcccgac tgggagttac tggccagctt
     1021 tggaatgccc tgggctactc gcaagcgtac cagccgtggc taatccaggc tgcgaactgg
     1081 ggaggcgtct acgcggtgag cttcctcatc gttacgatta acgcggcggt ggcctttctg
     1141 atcatcaatc gtagcaagcg gtcaatcatc gtcgcagcaa gcggggtcat cgcggtgatc
     1201 gtcgttgtct ttctgagccc aatacatgca gagcaggaaa cttcggtcgc cggcagagtg
     1261 gtcgcgttgc agccaaacgt tccgatggcg ctgatcaaat ccgccgaaga gatgcgcgaa
     1321 cttactgctc ggcacattac gatgagtgaa gaaggtctcg gccggcttcc gcatgacaca
     1381 acgccggtgc ttgtcatctg gccagagtcg ccaatgaact tcacctatgc aggcgatgca
     1441 cagtttcgtg aattcatctc cagctttgcg cgcgaacatg gcacatcgct cctctttaac
     1501 tcacaggaac cagctccgaa caacggcatc tacaattcgg ctgtgcttat aaatcgagac
     1561 ggtagcatgg ttggtcaata tgataagatt cgcctgctgc cgtttggcga atatgtgccg
     1621 ctaccccgct ggttgccggg agcgaatttg ataactgcca tcgttggaga cttctcgccg
     1681 ggcgtcaatt acacgctgtt gcccgcagga gaagcgaagg ctggggtttt catctgcatc
     1741 gagtcggcct atccgtcgat cgcacgcagg ttcaccgaag atggcgctga ggtgctaatc
     1801 aatatttcga acgacggtta tctgggacgg accgcggtat tgagacaaca cctcgccaac
     1861 gcgatctttc gcgcaactga aaacaggcga ccgctattgc gagttacgaa tacgggaatc
     1921 accgctttca ttaccaagaa tggcaaggta gtcgatgcca cagacgtttt tgagccggca
     1981 gtcagagtct gggatgtcaa acacaattct tcagacagga cgttttacac gcggcacggt
     2041 gatctgtttg cgcttagttg cgcagtggta acgttaatcg tctttaccct ggcattgtta
     2101 gccagaggca aagcacagag aagtaggatt tcggagaacg catgatcgat tcaatatcaa
     2161 ttcaggatct tagaaccaac tacgacgacc tctcgggtag agttgaacaa ctcgggaggt
     2221 ttctttgatg ttgccgctgg gcgcaatgaa ctagcacgca ttgaaaaaga ggcctcctcg
     2281 cctgattttt ggaacgacca ggaggcggca cagaagcttc tccagaagcg aagcctgctc
     2341 gaaaagaaac tccaacgtca ggagcgtttc gagtctcaga tcgccgatg
//
LOCUS       DASUEX010000474         3749 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_892728, whole genome shotgun sequence.
ACCESSION   DASUEX010000474 DASUEX010000000
VERSION     DASUEX010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3749
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_892728"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /gene="rpsD"
                     /locus_tag="VFV61_07965"
     CDS             <1..234
                     /gene="rpsD"
                     /locus_tag="VFV61_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="primary rRNA binding protein; nucleates 30S
                     assembly; involved in translational accuracy with proteins
                     S5 and S12; interacts with protein S5; involved in
                     autogeneously regulating ribosomal proteins by binding to
                     pseudoknot structures in the polycistronic mRNA; interacts
                     with transcription complex and functions similar to
                     protein NusA in antitermination; Derived by automated
                     computational analysis using gene prediction method:
                     GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEX4900452.1"
                     /translation="VDIPSFKVKAGDLISVREASRKNPHIEGAWQTAAGRGRPSWISQ
                     DGADMSVRISGLPSRDDIDRSINEQLIVELYSK"
     gene            303..1358
                     /locus_tag="VFV61_07970"
     CDS             303..1358
                     /locus_tag="VFV61_07970"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF003513.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEX4900453.1"
                     /translation="MAMDQNNLWTGFQMPRRLAADSETLTERYGRFSAQPFERGFGTT
                     IGNSLRRALLSSIEGAAVTAIKIEGVEHEFSSIKGVVEDATDVILNLKQVPFKLHGSE
                     AKTLRISKETAGEVTAADIETDADVEILDESVHIATVSDGGSLNVEMRLKRGRGYVSA
                     DRNFDEDLSLGYIPIDSVHTPVKKVNYTVEAARLGQNTEFDKLTIEVWTDGSVKPEDS
                     IGLAAKLIKDHMSIFINFEEDADDFSYSKMERPPLPRNDQLDRSVDELELSVRSYNCL
                     KNADIRTIRDLVQRSEREMLATKNFGKKSLNEIKDILNGMGLDFGMEFDEQGNPIPGS
                     GGRDTADAADFEAEEEQ"
     gene            1415..1876
                     /gene="rplQ"
                     /locus_tag="VFV61_07975"
                     /pseudo
     CDS             1415..1876
                     /gene="rplQ"
                     /locus_tag="VFV61_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010285110.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
     gene            2214..2849
                     /gene="accB"
                     /locus_tag="VFV61_07980"
     CDS             2214..2849
                     /gene="accB"
                     /locus_tag="VFV61_07980"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEX4900454.1"
                     /translation="MPEAKPTKMSEPENLNPQSDPQVEVESRDSSQPNRRRRQRSRQI
                     DPSVNMDELRELIDLIQENDFAEFEIAREGFRLHVRRGAAAAEVGRATGTSPPSEGSE
                     HAGGAAPPQTAGSTPSHSGAQARSVASEDQDLHMILSPIVGTFYRSPSPNADPFVRIG
                     SNVEAETIVCIIEAMKLMNEIQAEAAGEVVKIYVENGQPVEYGQPLFGIKR"
     gene            2928..>3749
                     /locus_tag="VFV61_07985"
     CDS             2928..>3749
                     /locus_tag="VFV61_07985"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEX4900455.1"
                     /translation="MSPENRKFKKILIANRGEIACRIIWTCKEMGIRTVAVHSDVDRD
                     SLHVRFADEAACIGPAPSAQSYLNIPAIISTAEIFNVDAIHPGYGFLAESSYFAEICE
                     ACNIKFIGPKSNIIRLMGDKIEARRVMKEAGLPVLPGSPDPISSEDEARKLADEIGFP
                     VIVKASAGGGGRGMRIVRSADDLGRALEAASTEAAAAFKDGSVYIERYLENPRHIEIQ
                     VLADEYGDCIHLGERECSIQRRHQKLLEEAPSPILSAELRNEMGNAAVAACKKLGY"
BASE COUNT          902 a          961 c         1105 g          781 t
ORIGIN      
        1 gtcgatattc cttcattcaa ggttaaagcg ggcgatctga tcagcgtcag agaagcaagc
       61 cgcaaaaacc cgcacatcga gggtgcctgg caaactgctg ccggccgcgg acgtccgtcc
      121 tggatctcgc aggatggcgc cgacatgagc gtgcgcatta gcggattgcc gtcgcgcgat
      181 gatatcgatc gctcgatcaa cgaacagctt attgtcgagc tttatagcaa gtaaattaat
      241 cacgcagatg tcgtcgtgga gacgacatct gcgcgctgac gtttctaacc aggacccaaa
      301 ttatggctat ggatcaaaat aatctttgga cgggatttca gatgccgcgt cgtctggcgg
      361 ctgactcgga aacgcttaca gaaaggtacg gtcgcttttc cgcgcaacct ttcgagcgcg
      421 gttttggtac gaccatcggc aactcgctgc gccgggcgct gctttcttca attgagggag
      481 cggctgtcac ggcgatcaag atcgagggcg tggagcacga gttctcttca atcaaaggcg
      541 tggttgaaga cgccaccgat gtgattctca acttgaagca ggttcccttt aagctgcacg
      601 gcagcgaggc gaagacgctg agaatttcca aggagactgc tggcgaggtc accgcggccg
      661 acatcgaaac tgacgcggat gtcgagatac tcgatgagag cgtacacatt gccactgtga
      721 gcgatggtgg ttcgctcaat gttgaaatga gattgaagcg cggacgtggt tatgtctccg
      781 cggatcgcaa cttcgatgag gacctttcgc tcggctatat tcccatagat tcggtgcaca
      841 ctccggttaa gaaagttaac tacacggtcg aggcggcccg tttagggcag aataccgaat
      901 tcgacaagct gacgatcgaa gtttggaccg atggctcagt taagcctgaa gattccattg
      961 gcctcgccgc gaagttgatc aaggaccaca tgtccatctt catcaatttt gaagaggacg
     1021 cggacgactt ttcttattcc aagatggagc gcccgccttt gccacgcaac gaccagttgg
     1081 atcgttctgt ggacgagctg gagttgtcag tccgttccta caactgtttg aagaatgccg
     1141 acattcgtac cattcgtgat ctggtgcagc gctccgaacg tgaaatgctc gccaccaaga
     1201 actttggcaa gaagtcgctg aacgagatca aggacatact taacggcatg ggtctggact
     1261 tcgggatgga gtttgacgag cagggcaacc cgattcctgg ttccggcgga cgcgacaccg
     1321 ctgatgccgc cgactttgaa gcggaagagg aacaataggg ctcgtcacac agcggttgat
     1381 tagaagatta ctaacttaga gctcgagtaa agtcatgcgg catcaaaagg cacatcggaa
     1441 attaggtcgg acgaccgagc atcgcatgtc gatgctccgc aatctggcga cttcgttgat
     1501 caacgcacgc gaagaacgaa tcgtcactac tctacccaaa gccaaggagt tgcgtccctt
     1561 tgtggagcgc gccattacgc tttcatgtcg cgcgcagcat ctcgacggcg atagcgcgaa
     1621 gctgagtgcg gtgcatttgc gccggcaggc ggcgggcttt tttcacgccg gcaacatgca
     1681 acaaacccag ttaaatggta aacgaggcca ggcgcgactg ccgcgcaccg ccggagtggc
     1741 cgcggtcaaa cgtctgtttg atgaactggg cgaacgttat aaggatcggc ccggtggcta
     1801 taccaggatc ttaaaactcg gcaaacgtga aggcgacaac gcagaattgg cgattattga
     1861 gctggtagac aatccgcgtg aactcgaagc ggcagaagct gccaggaaac gcagtaagtc
     1921 ggcaggccag aagaaaaaga aatcagatga cactcgcgga ggcgctgcgt ccaaagcgac
     1981 gaaggtcgag gctgcgcccg cgccggtggt agaggccgag cctgaaaccg ctgaagctgt
     2041 tgctgaggta gaagcggagg cgggtcagtc agaggaagag gcgaagtagt aaaacttgac
     2101 aatgcaccgg actgaatttg ctgctctgca gcccggtcgg cacaggcccc aatggaccat
     2161 cggcatcagg aaaccgagtc ttttggggct cgtcactttg aggagtttga ctgttgcctg
     2221 aagccaaacc aacaaaaatg agcgaacccg agaatcttaa tccgcagtcc gatccgcagg
     2281 tcgaagtgga gtctcgagac tccagccagc caaatcgccg gcgccggcag cgctctcgcc
     2341 agatcgatcc atcagtgaat atggatgagc tgcgcgagtt gatcgatctg attcaggaga
     2401 acgacttcgc agaatttgag attgcccgtg agggcttccg cttacatgtg cgacgcggtg
     2461 cggcggctgc ggaagtcggc agagccactg gcacttcacc gccgagcgaa ggatctgagc
     2521 atgcaggtgg agcagccccg ccccagacag caggatctac tccttcacat tccggagccc
     2581 aggccaggag cgtggcttct gaggaccagg acctgcacat gatcttgtct cccattgtgg
     2641 gcacctttta tcgctcacca tcgccgaatg cggatccctt tgtgagaatc ggcagcaacg
     2701 ttgaagccga aacgatcgtt tgcattatcg aagcgatgaa gttgatgaat gagatccagg
     2761 cggaggccgc tggcgaggta gtgaagatct acgttgagaa cggccaacca gtcgagtacg
     2821 gtcagccgct ttttggaatc aagcgatagc gttcaaagtt ggcgcccaat gtctccggtc
     2881 cccgcaggat acaggacatt gggcgttaag ttttgagtca aaacaaaatg agcccagaga
     2941 atcgaaagtt taagaagatt ctaattgcca atcggggcga gatcgcctgc cggattatct
     3001 ggacttgtaa agagatgggt attcgcacgg ttgccgtcca cagcgacgtc gatcgcgatt
     3061 cgttgcacgt gcgcttcgct gacgaggctg cttgcattgg accggcacct tcggcgcaaa
     3121 gttacctcaa cattcccgcc atcatcagca ccgcggagat attcaatgtc gacgcgatcc
     3181 atccgggtta tggttttctg gcagagtcgt cttacttcgc tgagatttgc gaggcgtgca
     3241 atatcaaatt cattggaccg aagtcgaaca tcattcggct gatgggcgac aaaattgaag
     3301 cacgtcgcgt gatgaaggaa gcaggtctgc ccgtgttgcc cggtagcccc gatcctatct
     3361 cttccgaaga cgaggcaagg aagctcgccg acgagatagg ctttcccgtg atcgttaaag
     3421 cttcagcagg tgggggcggt cgcggcatga gaatcgtacg ctctgctgat gatctgggtc
     3481 gcgctctgga ggctgcgtca acggaggcgg cggcggcgtt caaagacggc agcgtataca
     3541 tagaacgtta tctggagaat ccgcgtcaca ttgaaattca agtgctggcc gacgaatacg
     3601 gcgactgtat tcacctcgga gagcgcgagt gctcgatcca acgacggcat cagaaactct
     3661 tggaggaagc gccttcgccg atcttgtcag cggaactccg taatgagatg gggaatgcgg
     3721 ctgtggccgc ctgcaagaag cttggatat
//
LOCUS       DASUEX010000475         2819 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_178864, whole genome shotgun sequence.
ACCESSION   DASUEX010000475 DASUEX010000000
VERSION     DASUEX010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2819
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178864"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1178)
                     /locus_tag="VFV61_07990"
     CDS             complement(<1..1178)
                     /locus_tag="VFV61_07990"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX4900456.1"
                     /translation="MSINRTLAISGLILLSLMLLGCGSTKSTEKAAKAVRVKAVELHS
                     SASRVRYSASIRPAAQVDVAFKVSGYIDSIAQQKDPAGQWRHLQAGDVVRKGTVLARV
                     RQKEYLARVNEAQSQHGEARSALETTNSQLKEANAAVETARAQVNDAQAYFDRASLDF
                     ERARILFNTQSITKPDYDASKAQHEIAAAKLEAARGQLNSAEARVAISKAQINAAQSR
                     IKTADATTVSATIPLQDTQLRAPLTAVVIDRKVEIGALVSQGVTGFVLADLTSVKAAF
                     GVPDLALQSLKLGDTLKMTTDALPGAEFSGHISRISPSADQTSRVFDVEVLIHNTDGR
                     LKPGMIASLSLPEGASGAEVNVPVVPLTAITRAKDNPNAYAVLVVEEREGKQFAHLRS
                     V"
     gene            complement(1175..2533)
                     /locus_tag="VFV61_07995"
     CDS             complement(1175..2533)
                     /locus_tag="VFV61_07995"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX4900457.1"
                     /translation="MKRWMMTIFALVAFTGGELSVVCQQQLTDALTLDQAIKIALENN
                     RSVKNAKLEADKAGDKIGALRTRRLPSFKINSLISQPLSTFETNFAKGVFGTYAGIGP
                     VPAEDTVITSSTNTTGLIIAQISQPITQLRRIGLQIKQQQIGLEISKAGVTATEQSVV
                     NNVKRAFYAILQTQGQTQAAEESVKLHRELDRVTAEYVIQQVALRTDQMDVQTRLAKA
                     EFQVLTLSNQLRSQKEQLNLLLGRDVNTDFSVSDGFETAQVTMRETDLVEARQRALAQ
                     RPEIREARLRMRQADLDRRAKKSEFIPDVSVTANYISTFNYSNFIPRSISGIGVQVEW
                     EVFDWGRKKREVSEKARTVSQAENSLRETESQVLLEVNDRFRRLQETCQLIRIASLAQ
                     TQARANVQMASYKYRLEAVLLKDVLQAQTSLADADYEYQKALLSFWTAKADFEKAIGE
                     DK"
     gene            complement(2562..>2819)
                     /locus_tag="VFV61_08000"
     CDS             complement(2562..>2819)
                     /locus_tag="VFV61_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900458.1"
                     /translation="EDEHRNRIILKLEGTLTAADARLLEEICTDLKEDFGYGITIDFA
                     GVNFVGRQSAAILRRLKETPDLTMEGMHLFVQQIMESSETV"
BASE COUNT          552 a          799 c          742 g          726 t
ORIGIN      
        1 acactgcgca gatgagcgaa ttgtttgccc tcgcgttctt cgaccaccag cactgcataa
       61 gcatttgggt tgtctttcgc tcgagtgatg gccgtcaacg gcaccaccgg gacattcacc
      121 tccgcgccgc ttgccccttc aggcaaactt aacgaagcga tcatgccggg cttgaggcgg
      181 ccgtcagtgt tgtggatcag cacctcgaca tcgaacacgc ggctggtctg atctgcggat
      241 ggagaaatac gggagatgtg cccgctgaac tccgcgcccg gcaacgcatc agtcgtcatt
      301 ttgagcgtgt cgcccagctt cagactctgc agcgccagat caggaacgcc gaacgctgct
      361 ttaaccgaag tcagatcggc cagcacaaat ccagtaacac cctgcgagac cagcgcgccg
      421 atttctactt tgcgatcgat gaccaccgcg gttagcggcg ctcttagttg cgtgtcctgt
      481 agcggaatcg ttgctgaaac ggtcgtcgcg tccgcggtct tgatacgaga ttgcgcagcg
      541 ttaatttgag cttttgagat cgcgacgcga gcttccgcgg agttcagttg cccgcgggcg
      601 gcttccaatt ttgctgctgc gatttcgtgc tgcgcttttg acgcgtcgta gtcaggcttc
      661 gtgatgctct gagtgttaaa gagaattcgc gcacgctcga aatccaaact tgcgcgatca
      721 aaatacgcct gggcgtcgtt cacttgcgca cgcgccgttt cgactgcggc gttcgcttct
      781 tttaactgag aattggtagt ctcaagcgcc gaacgcgcct cgccgtgttg agattgagct
      841 tcgtttactc gtgccaggta ttccttctgc cgcacgcgag caagcacagt ccccttgcga
      901 accacatcgc ctgcttgcag atgccgccac tgacccgcag gatctttctg ctgggcaatc
      961 gaatcgatgt agccactaac cttgaaagca acatcaacct gagcggcggg cctgatgctg
     1021 gctgagtagc gaactctgct cgcggatgag tgcagctcaa cagccttgac tctaaccgcc
     1081 ttcgccgcct tttctgtgct cttggtgctt ccgcaaccca gaagcatcaa gctgagtaag
     1141 atcaaaccgc tgatcgcgag tgttctgttg atactcattt gtcttctcct attgcctttt
     1201 cgaaatcggc tttcgctgtc cagaacgaca agagtgcttt ttggtattca taatcagcat
     1261 cggcaagaga cgtctgggcc tgcaggacgt ccttcagcag gaccgcctca agacggtact
     1321 tgtagctggc catttgcaca ttcgcgcgtg cctgagtttg tgcgaggctg gcgatgcgaa
     1381 tcagttggca agtctcctgc aagcggcgaa agcgatcgtt cacttccagc aacacctggc
     1441 tctcggtttc gcgcaaactg ttttcagcct ggctaaccgt tcgcgccttt tcactcacct
     1501 cacgcttctt tcttccccaa tcaaaaactt cccattccac ttggacgcca ataccgctga
     1561 tgctccgcgg aataaagttc gaatagttaa atgtcgaaat gtaattggcc gtgacactca
     1621 cgtcgggaat gaattctgat ttctttgcac gtcgatcaag gtcggcctgc cgcattctca
     1681 ggcgcgcctc gcgaatctcc gggcgctgtg ccagcgctcg ttgtctcgcc tcaactaaat
     1741 cagtctcgcg catggtcacc tgtgcggtct cgaatccatc gctgacgcta aagtcggtat
     1801 tcacatcgcg gccgagcagc aggttcaatt gctccttctg ggacctaagc tggttcgaca
     1861 gcgttaggac ctggaactca gccttggcga gccgcgtctg gacatccatc tgatctgttc
     1921 gcagcgctac ctgttggatt acatattccg cggtgacgcg atccagttcg cgatggagct
     1981 tgactgactc ttccgcagct tgcgtttgtc cttgtgtttg cagaatggcg tagaaggcac
     2041 gcttcacgtt attgaccacg ctttgttccg tggcggtcac tccggccttg gagatttcca
     2101 gtccgatttg ctgttgtttg atttgcaagc cgatccggcg aagttgcgtg atgggctgac
     2161 taatctgcgc gatgattagc ccggtagtgt ttgtcgacga ggtaatgact gtgtcttcgg
     2221 caggtaccgg accaatgccc gcgtaagtcc cgaagacacc ttttgcaaag ttggtctcga
     2281 aagtgctcag tggttgcgaa atcaggctgt ttattttgaa ggagggcagg cggcgtgtgc
     2341 gtagcgcgcc aattttgtcg cccgccttat cagcttctag tttggcgttt ttcaccgagc
     2401 ggttattttc cagcgcaatc ttaatggcct gatccagcgt cagcgcatcg gtaagctgct
     2461 gctggcagac aacggaaagt tcgcctcccg taaatgcaac cagcgcgaaa attgtcatca
     2521 tccagcgttt cattattggg cgcctccttc ctttgccttt attaaactgt ttcagacgac
     2581 tccatgattt gctgcacaaa caagtgcatt ccctccatcg ttaggtcggg tgtttctttc
     2641 aggcggcgca agatagccgc gctctggcga ccaacgaaat tgacgcctgc aaaatcgatc
     2701 gtgattccgt agccgaaatc ctctttgagg tcggtgcaaa tctcttccag caaccgggca
     2761 tcggctgcgg taagcgtgcc ttccagcttg agaatgattc ggttcctgtg ctcgtcttc
//
LOCUS       DASUEX010000476         2301 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1127862, whole genome shotgun sequence.
ACCESSION   DASUEX010000476 DASUEX010000000
VERSION     DASUEX010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1127862"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            240..1829
                     /locus_tag="VFV61_08005"
     CDS             240..1829
                     /locus_tag="VFV61_08005"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEX4900459.1"
                     /translation="MTTSRREFIKTAGVAAGVAILPSSLPSWISDVEAAEAAAAEKVN
                     KSALADIALNQARKLGCSYADIRINRYRNESISTREKQVQNVSRSQNFGFGVRVLFKG
                     TWGFASSRDVTPQEIWRVTRQAVDIARANSVYQRRRVTLERVPKVQANWRSSFEKDPF
                     EVPIDNKIDLLLKLNERALRTDGVSFVSSSMSFVNEQKFYASTDGARIEQYIIRANPN
                     LNVTAVNRANGDFQSRNSLGGPQGLGYEYMEKYPWLKEAEQAGEDAVAKLKAKSVDPG
                     KYDLVLHPSHLWLTIHESVGHPTELDRSLWWEANYAGTSFLTRDKIGKLKFGSSIVNF
                     LADRTQRGGLATVAYDDEGVPGQEWPLIKDGVFVDWQTTRDLAAMSPGKKSYGCLHAD
                     SWGSVSFPRMPNVSLQPAAGSTTMDDLIGGVERGILIYGDGSYSIDQQRYNFQFGGQT
                     FWEIKNGKIGGMLRDVAYQSRTTDFWGSCDGLGGRSTYELGGSFNDGKGEPGQSNAVS
                     HGCPPARFRKVNVLNTAAKST"
     gene            1925..>2301
                     /locus_tag="VFV61_08010"
     CDS             1925..>2301
                     /locus_tag="VFV61_08010"
                     /inference="COORDINATES: protein motif:HMM:NF013673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase modulator"
                     /protein_id="HEX4900460.1"
                     /translation="MLLTEQEARALTEKLLSYVRADDAIVSVNSENYSHLRFAANAFS
                     TSGARKNTSVGVTVWIDKKRGASSTNEIDDASLRAAVAQAEEIARISPVDREYLPTLG
                     RQTYKPVAGYSEATSNISLQDRAR"
BASE COUNT          531 a          589 c          673 g          508 t
ORIGIN      
        1 tgaatttaga gcctcgaact ttgttcggct gatgaaactt gagcggcaag aagttactga
       61 tcgagctgct taagcaaagt actaagcacg aagtcccaag ttcgttttat tttctcggcg
      121 aactctgcgg tgcttggcgt cttggcggtg ttagttttct agatgaaatg ttgacaacat
      181 tagcagcgca gcgttagatc atcccgcccg tcaaatccca tcctgcaaag gttttgttca
      241 tgacaacctc tagacgcgag tttatcaaga cagcgggcgt cgctgccggc gtggcgatct
      301 taccgagttc gttaccgtcc tggattagcg acgttgaggc cgcggaagcc gctgctgccg
      361 agaaagttaa taagagcgcg ctggcagaca ttgccctgaa tcaggcgcgc aagctgggct
      421 gttcttacgc ggacattcgc atcaatcgtt atcgcaacga gtcgatctcc acgcgtgaga
      481 aacaggtgca gaacgtttcg cgcagtcaaa actttggttt tggcgtgcgc gtcctgttca
      541 agggcacgtg gggatttgct tccagccgcg acgtgacgcc gcaggagatt tggcgtgtga
      601 cgcgtcaagc tgtcgacatt gcccgcgcca attcggttta ccagcggcgg cgagtcacgc
      661 tcgaacgcgt tcccaaagtc caggcgaatt ggcgcagctc gtttgaaaag gatccgttcg
      721 aggtacccat cgataacaag atcgatctgt tgttgaagtt gaacgagcga gcgctaagaa
      781 cagacggagt cagcttcgtt agctcttcga tgagttttgt taacgaacag aaattttacg
      841 cgtcgacgga tggggcacgc atcgagcagt acatcattcg cgctaacccg aacctgaacg
      901 tgacggccgt gaatcgcgcc aacggcgatt ttcagtctcg caattcactc ggcggcccac
      961 agggtctcgg ttacgagtac atggagaaat atccctggct gaaggaggct gagcaggccg
     1021 gcgaagatgc ggtggcgaag ctgaaagcga agtcggtgga tcctggcaaa tacgatcttg
     1081 tcttacatcc ctcgcatctg tggctgacga tacatgagtc ggtaggccat cccacagaac
     1141 tcgatcgctc gctctggtgg gaagccaact atgccggcac cagtttctta acgcgcgaca
     1201 agatcggaaa gctcaagttc ggatcgagca tcgttaattt tctggcggat cggacgcaac
     1261 gaggcggtct ggcaactgtc gcctacgacg atgaaggtgt accgggtcag gagtggccgc
     1321 tgatcaagga tggagttttt gtggattggc agacaacccg agatctcgcc gcgatgtcgc
     1381 ctggcaaaaa atcttatggc tgtctgcacg cagacagttg gggcagcgtt tcttttcccc
     1441 gcatgcccaa cgtctcgctg caaccagcgg cggggagcac aacgatggat gatttgattg
     1501 gcggggttga acggggaatc ctgatttacg gggacggcag ctactccatc gatcagcagc
     1561 gttataactt ccagtttggc gggcagacat tctgggaaat caaaaacgga aaaatcggcg
     1621 gcatgttgcg ggatgtggcc tatcaatcgc gcacgactga cttttgggga tcgtgcgatg
     1681 gcctgggcgg taggtcgacg tatgaactgg gcggcagctt caacgacggc aagggtgagc
     1741 cgggccaatc gaatgcagtg agccatggtt gcccgccggc gcgctttcgc aaagtgaatg
     1801 tgttgaatac tgcggcgaag agcacctgac cattcgtctc gcttagaagt taagttgtgg
     1861 aatttactct ggcggcggca agccggcctt ccggaccctg aggttccaat cacggagcta
     1921 accaatgttg ctaacagaac aggaagcgcg cgcactgacg gagaagttgc ttagttacgt
     1981 tcgcgcggac gatgccatag tcagcgtcaa cagcgaaaac tattcgcact tgcgctttgc
     2041 ggccaacgct tttagcacca gtggcgcacg caagaacaca tctgttggtg tcaccgtttg
     2101 gatcgataag aaacgtggcg cctcttccac gaacgagatt gacgacgcct cgttgcgagc
     2161 cgcggtagcg caagccgaag aaatcgcccg catttcccca gttgatagag aatacctgcc
     2221 cacgctgggc cgccaaacct acaaacctgt tgccggctat tcggaggcga cgtcaaatat
     2281 ttcattacaa gatcgggcac g
//
LOCUS       DASUEX010000477         2240 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1219638, whole genome shotgun sequence.
ACCESSION   DASUEX010000477 DASUEX010000000
VERSION     DASUEX010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1219638"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="VFV61_08015"
     CDS             complement(<1..424)
                     /locus_tag="VFV61_08015"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HEX4900461.1"
                     /translation="MQIEILLTILLLFVLSLLATVDMAFSQLTDVGLRRLISEAEEHP
                     TPRSSFLRQVLENRQRFSFTLTAAIQILLVVVAVLITSISLSLFSNTRYVLVGLATGL
                     VLAGIFRQLIPLLLSHRNPEATFLFLLPLVRPFFRVMAF"
     gene            complement(593..1012)
                     /gene="ybeY"
                     /locus_tag="VFV61_08020"
     CDS             complement(593..1012)
                     /gene="ybeY"
                     /locus_tag="VFV61_08020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEX4900462.1"
                     /translation="MIEVVNRQRKVRIDPERWRPFSEKALTAIRRAADDATIAFVSDR
                     QIKELNRRFRGIAKATDVLSFPAGEETTAPNANLGDIAISLERAADQAKENKLTLDDE
                     IAQLILHGLLHLCGYDHETDNGEMNRLERKLRAKLKI"
     gene            complement(1036..1989)
                     /locus_tag="VFV61_08025"
     CDS             complement(1036..1989)
                     /locus_tag="VFV61_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006681752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEX4900463.1"
                     /translation="MRKIELTPRGLETLFGVHDQNIKYLESLLDVRINARGQDVSIDG
                     DPKDVETAEGILDDFSALFQEGRQFTDRELREAFAQIAEDRAFSLRDYYTKSRFNPAG
                     KKQVAPKSATQRKYIQTIQDRDVVFGIGVAGTGKTYLAVAMAVQALMQKQVNRIVLAR
                     PAVEAGEKLGFLPGDLQDKVDPYLRPLYDALFDLIDYERVTRLLEKRVIEVAPLAFMR
                     GRTLSDAFIILDEAQNTTSEQMKMFLTRIGFGSKAVITGDVTQVDLPAGKRSGLIEAE
                     RVLSNLEGIEFVYFTEKDVVRHKLVQMIIRAYEDHTKKSNI"
BASE COUNT          532 a          566 c          620 g          522 t
ORIGIN      
        1 cgaaggccat cacgcggaag aagggccgga cgagcggcaa tagaaacaga aaggtggctt
       61 ccggattccg atgcgacaat aagagaggaa ttaattggcg aaagataccg gccagcacca
      121 acccggttgc caggccgacg aggacgtagc gagtattgga gaaaaggctg agcgagatcg
      181 aagtgattag gaccgcaacc acaacgagca ggatctgaat ggcggcggtg agtgtgaaac
      241 taaaacgctg gcgattctcg agaacctggc gcagaaacga tgagcgggga gtcggatgct
      301 cctcagcttc gctgatgagt cgccttagtc cgacatcggt gagttggcta aacgccatgt
      361 caaccgtggc aagcaaagac aggacaaaaa gcagcagaat cgtaagcaat atctcaattt
      421 gcatgtgctg ctagatagta gtgcctaaac gctcgtttgt cagtagtaag agagtccccg
      481 ggccacttga ccgcaaatgg caatcaccaa acaggcttgt ttggcgagca acgaataatg
      541 gtttcaactc actcgcttca cggtggtgct cagtgggagt acagggcgtt catcaaatct
      601 taagctttgc acgtagttta cgttccaacc ggttcatctc gccattgtcc gtttcgtggt
      661 cgtaaccgca gaggtgtaac aggccgtgca ggatcagctg agcgatctca tcgtccagtg
      721 taagcttgtt ttccttagcc tgatccgcgg cgcgctcgag ggagatggcg atgtctccga
      781 ggttagcgtt gggagcggtg gtttcttcac cagcgggaaa cgacaagaca tcggtcgcct
      841 tagcgattcc ccggaagcgg cggttcaatt ccttaatttg ccgatccgaa acaaaagcga
      901 tcgtggcatc gtcggccgct ctgcgaattg cagtcagcgc tttttcgctg aagggcctcc
      961 agcgctcagg atcgatgcga actttacgtt gccggttaac tacttcgatc atgaatacgg
     1021 aacccagggc gcaggctaga tgttgctctt cttggtgtga tcttcgtagg cgcggatgat
     1081 catctgcacc agcttatgac gtacgacatc tttttcggtg aagtaaacaa actcgatccc
     1141 ttccaaatta gagagcactc gctcagcttc gatcaaacca ctgcgcttgc cggcgggcag
     1201 gtcgacctgg gtgacgtcgc ccgtgatcac cgctttcgat ccaaagccga tacgtgtcag
     1261 aaacatcttc atctgctcgc tcgtcgtatt ttgcgcttca tccaggatga tgaaagcatc
     1321 cgaaagagta cgaccgcgca tgaaagcaag cggtgcaact tcaatcactc gcttttccag
     1381 aagcctggtc acgcgttcgt agtcgattag atcaaataag gcgtcgtaca gcgggcgcag
     1441 gtagggatcc actttgtcct gcaaatcacc gggcaggaat cccagctttt ctccagcttc
     1501 gacggcggga cgagcgagga ctatgcggtt gacctgcttt tgcatcagcg cttgaacggc
     1561 cattgccacc gccagatagg ttttgcccgt tcccgcaaca ccaatcccaa acacaacgtc
     1621 acgatcctgg atggtctgaa tatatttccg ttgggtggca gacttgggcg ctacttgttt
     1681 cttgccggcg ggattgaaac gcgacttggt gtagtagtcg cgcaaactga aggcgcgatc
     1741 ctcggcaatc tgggcaaacg cctcgcgtag ttcgcgatcg gtaaattgac gtccttcctg
     1801 aaacagcgca gaaaagtcgt cgaggatacc ttcggctgtt tccacatctt tgggatcccc
     1861 atcaatggaa acatcttgtc cgcgtgcatt gatacgcacg tcgagcaggg attccagata
     1921 cttgatgttc tggtcgtgga caccgaacag agtttccagt ccccgaggcg taagttcaat
     1981 cttcctcaag aactctctct ttccaattca gctggataat gtagaggtga tgctgcgaga
     2041 gcatggggta ggatcatcga catatgcttc attgtggacg ctactatagc gttcgcgggc
     2101 atgtcaaatg gcggcaatgc cgtcatcaaa ctgtaggcgg cattcaattg ccgattggcg
     2161 attgccaatt gccgattagc cttgagtggg ctgagtctat actactggtg gatcttggga
     2221 cttgagactt gagacttggt
//
LOCUS       DASUEX010000478         2366 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_89412, whole genome shotgun sequence.
ACCESSION   DASUEX010000478 DASUEX010000000
VERSION     DASUEX010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89412"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /locus_tag="VFV61_08030"
     CDS             complement(<1..529)
                     /locus_tag="VFV61_08030"
                     /inference="COORDINATES: protein motif:HMM:NF017019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF711 family protein"
                     /protein_id="HEX4900464.1"
                     /translation="MEYSQAEILQTVAMTEMEHLDVRTVTLSLSLRDCAAETIARTAD
                     RVYEKIMRVGERLVATVDQIGDEFGIRVANKRIAVTPVSMITEGASGDPIELARTIDA
                     AAKSVGVDFIGGYSALVHKGATDSENQFLNSIPEALATTERLCSSVNVATTRAGINMN
                     AVRQVGELIKDAAERT"
     gene            complement(584..1132)
                     /locus_tag="VFV61_08035"
     CDS             complement(584..1132)
                     /locus_tag="VFV61_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900465.1"
                     /translation="MSDPEIARRCRSCGASIRVRGLFCPQCGKPTSEPGSDASSHEPA
                     IDTEEVYVAPETVSVDLPSLPTSDFSNDHPFRTAETVPLQPLRNDVEDSVQRFKESAT
                     TRFAVNTHYSPAEAREGEVRPRSDRETLGGDVKKRLGKMREISTVMLEEASYDPSARF
                     VLVAAVLFVLFLVILLLSEMMR"
     gene            complement(1286..1765)
                     /locus_tag="VFV61_08040"
     CDS             complement(1286..1765)
                     /locus_tag="VFV61_08040"
                     /inference="COORDINATES: protein motif:HMM:NF001220.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEX4900466.1"
                     /translation="MPNEDLIYRITRTVYDRLGAAAEEQAVEQLVTDIYRQVEPFLAS
                     NGTSYSSRQNLPAAGSVQSQSGEGSPDRLIISVFGVDHPGIVAGVAQILADAECSIVD
                     INQTVVQGKFAMVIVGDATRARQSTNELKERLRQEGEQLGVRIYAQREDLFNAMHRI"
     gene            complement(1922..2254)
                     /locus_tag="VFV61_08045"
     CDS             complement(1922..2254)
                     /locus_tag="VFV61_08045"
                     /inference="COORDINATES: protein motif:HMM:NF015289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HEX4900467.1"
                     /translation="MFLAKVVGTVWSTKKTPDLEGVRFLVVHPYDLDKEPTRNIVVVA
                     DRLGAGAGEMVMCAFGKAARSAIGNQDMAIEAAVVGIVDRVDLQESVSEEMRDAAREL
                     SRDNGKKS"
BASE COUNT          534 a          643 c          614 g          575 t
ORIGIN      
        1 gagtgcgctc ggcggcatcc ttgatcagtt cacccacctg gcgtaccgca ttcatgttga
       61 ttcccgcacg tgtggtggca acgttgactg aggaacacag tcgttccgtt gtcgcgaggg
      121 cttcgggaat tgagttgaga aactggtttt ccgaatcggt ggcgcctttg tgcaccagcg
      181 ctgaatagcc cccaatgaaa tcgacgccaa cagacttcgc cgcggcatcg atggtgcgcg
      241 ctaattcaat cggatcaccg gaggcgcctt ccgtgatcat ggagacaggc gtaacggcga
      301 ttcgtttatt tgcgacgcgg atcccgaatt catcgccgat ctgatcgact gtggcgacga
      361 gacgctcgcc cacccgcatg atcttttcat agacacgatc cgccgtgcgc gcgattgtct
      421 cggcagcaca atcgcgcagg ctcagactga gtgtgacggt gcgcacgtcg agatgttcca
      481 tctcggtcat cgcgacagtt tgaaggattt cggcttgtga atattccata gaagggtttc
      541 gggttccggg tgtcaggtgt cgggtctcaa atcgaaacct tcctcatctc atcatttcgc
      601 tcaacagcaa aatcaccaga aacaaaacga acaacaccgc cgctaccaga acgaagcgtg
      661 cgcttggatc gtatgaggct tcttcaagca tcacagtgga gatctcccgc atctttccca
      721 gccgcttctt cacatcccca ccgagcgttt cacgatccga gcggggacga acctcgcctt
      781 ctcgcgcttc cgccggcgaa taatgagtgt taaccgcaaa gcgcgtggtc gcggactcct
      841 taaatcgctg aactgaatct tcaacgtcat tccttaaggg ttgcaaagga accgtctctg
      901 cagttctaaa agggtggtcg ttcgagaagt cggatgtcgg caggctgggc agatctaccg
      961 aaacagtttc aggtgcaacg taaacctctt ctgtatcaat tgctggctcg tgacttgaag
     1021 catcggaccc tggctcagac gttggcttcc cgcattgcgg gcagaataag ccgcggacac
     1081 ggatcgatgc tccgcaggaa cgacaacgac gagcaatttc tggatctgac atgtcagctt
     1141 aggaagtcga aggttgtctg tttttgagct ttgttcttag tactttgtcc tttgttcgcc
     1201 aaggtaactt atttgatctc tgtcagaatg tacgaaggac ccagtaaaga aactaagcac
     1261 aaagaactaa gtacaaagtt cgttttcata tccggtgcat cgcgttgaag agatcttcgc
     1321 gttgcgcgta aatgcggacg cccagctgct cgccttcctg acgcagtcgc tctttcaact
     1381 catttgtgct ctggcgcgcg cgcgtggcgt cgccgacgat caccatcgca aattttccct
     1441 gcactacggt ttgattgata tcgacgatgc tgcattcagc atcggcaagt atttgcgcca
     1501 ctccggcaac aattccggga tgatcgacgc caaagaccga aatgatcagg cggtccggcg
     1561 aaccttcgcc tgattgcgac tgcacgcttc cggctgctgg caaattctgt ctgcttgaat
     1621 atgaggtgcc gttgctggcg agaaaaggtt cgacctggcg gtagatgtcg gtgacgagtt
     1681 gctcaaccgc ttgttcttca gcagccgcgc ccaaccggtc atacaccgtg cgggtgatgc
     1741 gataaatcaa atcttcattc ggcatttatg acaggtagtg gatagctatc atgctttctt
     1801 tttgaacttt gtgctttgaa ctttgtactt tgactgccct actggaacca gaggtcttcg
     1861 gcagatttct caacgaagct caaagtacaa agttcaaaga tcggagttaa caagctgagg
     1921 ttcaagactt ctttccgttg tcgcgactca attcgcgagc ggcatcacgc atttcctcgg
     1981 aaactgattc ttgcaaatct acccgatcga caatcccgac gactgcggcc tcgatcgcca
     2041 tgtcctggtt gccaatcgcg gagcgcgccg ctttgccaaa ggcgcacatc accatctcgc
     2101 ctgctcccgc gcccaggcga tcggcaacta ccacaatgtt gcgagtcggc tccttatcca
     2161 gatcgtaagg gtgcacgacg agaaacctga caccctcgag atccggagtt ttcttggtcg
     2221 accaaactgt gccgacaact ttagctagaa acatggctcg tgaatcggga atcgggaatc
     2281 gttaatcgtg aatcgtggat cgtgaaatcg tggatcggaa tccggaatcc gaaatccgga
     2341 atgctcgcgc gaatcgattc acgatt
//
LOCUS       DASUEX010000479         2089 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_894734, whole genome shotgun sequence.
ACCESSION   DASUEX010000479 DASUEX010000000
VERSION     DASUEX010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_894734"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1338)
                     /gene="dnaG"
                     /locus_tag="VFV61_08050"
     CDS             complement(<1..1338)
                     /gene="dnaG"
                     /locus_tag="VFV61_08050"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEX4900468.1"
                     /translation="MRYPQTFIDDLKRQADIVRVIQDYVTLKKKGANWMACCPFHKEK
                     TPSFSVSPAKEIFYCFGCHKGGTVFNFVMEMERVSFPEAIRLVAEKSGVPLPKLELDS
                     RFEARRSEIDDVIQLNKWALEWWEQQLEHESGEAKSARDYLQQREISDETRKTFRLGF
                     APDSWDALSSHLRQKGAAQDQIDKSGLVARKEDTGRTYDRFRGRLMFPVMDIQGKPIA
                     FGGRTLKNDDAKYINSPETAAYIKGRNLFGLNLTRDEIRRQGYVILVEGFLDLIVPFQ
                     FGIRNVAASLGTALTSDQVKLLGRFARRVVVNYDGDRAGVQAAKKAIEILLTEDLEVK
                     VLVLPDNADPDEFIRKFGASEYQRRRGEAQPHIQFVIDQAVRDRKLSNPAHKEAAIEE
                     VLPFIRAVRSRIQKREYFDIAMDSLRVSDLSLRREFWQSLRGSETARSVVKRGA"
     gene            complement(1399..>2089)
                     /locus_tag="VFV61_08055"
     CDS             complement(1399..>2089)
                     /locus_tag="VFV61_08055"
                     /inference="COORDINATES: protein motif:HMM:NF013844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HEX4900469.1"
                     /translation="REAQRRVSEMKREAQKASASGRPRAVIAADASTQSRGVRIVRDG
                     SVVTANPVASKTTSKGDEERYVAAPAREIVVGDKVKLRSFGSIGIVDSIKDDEVELRV
                     KSLRMREKLENLELVEAVAPPKTGGRFSKLRETRGPELKLGLSEAASASELNVIGQRT
                     DEAVDAVDKFLDAASLAGTSQLRIVHGHGTGALRRAIGELLKDHPHVARFTAAPQDQG
                     GSGATVVELRQ"
BASE COUNT          438 a          635 c          526 g          490 t
ORIGIN      
        1 ggcgccgcgc ttaaccacag atcgagcagt ttcgctgccg cgtaacgact gccagaactc
       61 tcgccgcagc gacaggtcgc tgacgcgcaa cgagtccatt gctatatcaa agtactcgcg
      121 cttctgaatc cggctgcgga cagcgcgaat aaacgggagc acctcctcaa tcgcggcctc
      181 tttgtgggcc ggattgctga gtttgcgatc ccgtacagcc tgatcgatca cgaattgtat
      241 gtggggttgt gcctcgccac gccgacgctg atactcgctt gcgccaaact ttctgatgaa
      301 ttcgtccgga tctgcgttgt ccggaagcac cagcacctta acttcaagat cttctgtcag
      361 aaggatttcg atagcttttt tcgccgcctg cacccccgcc cggtcaccat cataattgac
      421 taccacccta cgcgcaaacc ggcctaatag tttcacttgg tcagatgtaa gggccgttcc
      481 caaactggcg gccacattac gaatgccaaa ctgaaagggc acaatcaggt cgaggaagcc
      541 ctcaaccaga attacataac cctgccggcg gatttcgtcg cgcgtgaggt ttaagccgaa
      601 aagattccga cctttgatat acgccgccgt ctctggcgag tttatgtact tcgcatcatc
      661 gtttttgagc gtgcgaccgc caaaggcgat tggtttcccc tggatgtcca ttaccgggaa
      721 catcaagcgc ccgcgaaacc gatcgtaggt gcggccagta tcttccttcc tggctaccaa
      781 tccgctttta tcaatctgat cctgagccgc tcctttctgg cgcaagtgac tggaaagggc
      841 gtcccaactg tcgggagcga aacctaaacg aaatgtcttt cgtgtttcgt cgctgatctc
      901 ccgctgctgc aggtaatcgc gcgcgctctt agcttctccg ctctcgtgct ccagttgctg
      961 ttcccaccac tcgagcgccc acttattgag ttgaatgaca tcatcgatct cgctgcggcg
     1021 agcttcaaag cgactatcca gctccaactt aggcaaaggc actccgctct tttccgcgac
     1081 gagcctgatc gcttccggaa aggaaacgcg ctccatttcc ataacgaagt taaagacagt
     1141 gccgcccttg tggcagccga agcaatagaa aatctctttg gcaggactaa cagaaaaaga
     1201 gggagttttc tctttatgaa aagggcaaca agccatccag tttgcgccct tctttttcag
     1261 ggtcacgtag tcctggatca ctcgaacgat gtccgcctgg cgtttgaggt cgtcgatgaa
     1321 tgtttgggga taacgcattt gaagacgaac gtgagtaaaa cgttagaacc tttaatcggt
     1381 caaccgctgg tcgttgaact actgccgcaa ctctacaact gtcgcaccgc tgccgccttg
     1441 atcctgaggc gccgcggtaa agcgcgccac gtgcggatgg tccttgagca gttcaccaat
     1501 ggcgcgacgc aacgcgccgg tgccgtgccc atgaacaatt cgtaactggc tggtgccggc
     1561 gagtgaagcg gcgtcaagaa acttgtcaac tgcgtccact gcttcatcgg ttctttgccc
     1621 aataacattc aactctgaag cgctcgcagc ctcagacagg cccagtttca attccgggcc
     1681 gcgcgtttcg cgcaacttgg aaaaccggcc gccggtcttg ggcggcgcca ccgcttccac
     1741 tagttccaga ttctccagct tctctcgcat tcgcagcgac ttgacccgca gttcgacctc
     1801 gtcgtctttg atgctatcga cgattccgat tgagccgaac gagcgtagct ttaccttatc
     1861 gccaaccacg atctcccgag ctggagccgc gacgtagcgt tcctcatcgc ccttgcttgt
     1921 cgttttggac gccaccggat tcgccgttac aacgctgcca tcgcgaacga tccgcactcc
     1981 gcgagactga gttgacgcat ccgcggcgat gactgcgcga ggacgaccgg aagctgatgc
     2041 tttttgcgct tctcgtttca tttccgaaac tcgtcgctgc gcctcgcgc
//
LOCUS       DASUEX010000480         3061 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1671735, whole genome shotgun sequence.
ACCESSION   DASUEX010000480 DASUEX010000000
VERSION     DASUEX010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3061
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1671735"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(280..1002)
                     /locus_tag="VFV61_08060"
     CDS             complement(280..1002)
                     /locus_tag="VFV61_08060"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX4900470.1"
                     /translation="MEPQAASKYADKRLLIVDDDADMRLLLAEYFRRLGFQVDERESG
                     AAALEPAVGGRFDCFIFDVSMPGMTGFELLKRVRGRGVQTPTLFLTAHDALDYKVAGF
                     EAGADDYLAKPFSPRELEYRVEALLRRSGMVQEPHDADRIEVGNLVIDKRRHEVSREG
                     SRVDLTPLEFQILELLASEPGRAWSRNALLDQVWSTDYEGYQRNIDPHINRLRKKLEV
                     DPKNPRYVLTVRGVGYKLNEAP"
     gene            1275..>3061
                     /locus_tag="VFV61_08065"
     CDS             1275..>3061
                     /locus_tag="VFV61_08065"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEX4900471.1"
                     /translation="MNDFRRLLRYLKPHWGIFILATIAMLIVGLLEGAIGALIVPIFN
                     QAFAQGAGQRTATLFGLERIIPDSGLAAWKTISILLLTFTLAKGVAEYFSTYLMARIG
                     QSAVLQLRQDLYRHVLLQSASFFERHRTNYLVSRLVSSAAAIEAAVTSTIRDMLRESF
                     TLIVFLGASFFYSWRLTLGSLLLAPMVAVLTARFGTRLRNLARDSFEGSKELTDTAQE
                     ALSNQSIVKAYRGEAREAERFTTVAQRIVRANLRTARISGFAPPSIEMLGVVFIVFLL
                     YFGRHEIVSGRMNAAQFLAFLFFLFRSYDPMRKLSRLQNSMEQALAAAHHVWEVMDEH
                     VEIPERADAVELQPLKRGIELRDVSFGYANEGRPVLRNVSLQVAAGTMVALVGESGGG
                     KSTLTKLLPRFHDPISGAVLWDGVDLRKVRLASLRKQIALVTQETVLFNDTVLHNISY
                     GKPEATQAEVEAAAKIAFAHDFIMELPHGYQTIVGERGVFLSGGQRQRLAIARSLLVD
                     APLLILDEATSALDAESERFVQQAIANLVRNRTTLVIAHRLSTVRRADKIVVMEAGRI
                     IETGSHEELLARGGQYRKLYELQFADEENS"
BASE COUNT          714 a          837 c          809 g          701 t
ORIGIN      
        1 atgtggacaa acccaccctt gtgtaagaca aacttcagaa cgaaccaaac caccaacaga
       61 ctcgacccaa cgatccacat gtttctggtt taccaactcc caaccggctt attcaatacg
      121 tctgagtcga cgcgaatttc ccaacactga actccagcga caattggaga ggccaagaat
      181 agttgaaaac agaatggcgg cggcgaaact tcggagacaa ttggcagaga tactggtcgg
      241 gattcgtcaa acattcgaga acgccttagc gaattcggct cagggcgctt catttagctt
      301 gtaaccaaca ccgcgcacag ttaagacgta acgaggattc ttcggatcaa cttcaagctt
      361 tttccgcagc cgattgatat ggggatcaat gtttcgctgg tagccttcgt aatcggtact
      421 ccaaacttga tccaataaag cattacgcga ccaggcccgc cccggctcgg aagcaagtaa
      481 ttccaaaatt tgaaactcaa gcggcgtcag atccactcga gagccctcgc ggcttacttc
      541 atgccgccgc ttatcaatta cgagattgcc aacttcgatg cgatctgcgt cgtgcggctc
      601 ctgaaccatc cccgagcgcc gcagcaaagc ctcgacccga tactcgagct ctcgcggact
      661 aaacggtttg gcgaggtaat cgtcggcgcc tgcttcaaag ccggctactt tgtaatcgag
      721 agcatcatgc gcggttagaa acagagtggg tgtttgcaca ccgcgtccgc gcactcgctt
      781 cagtaactca aagccggtca ttcccggcat ggagacatcg aagatgaaac aatcgaacct
      841 gcctccgacg gccggttcga gagcggcggc gccactctct cgttcatcaa cctgaaaacc
      901 caaccggcga aagtattcgg ccaggagcag acgcatgtcg gcgtcgtcat caacgataag
      961 aagtcgcttg tcggcgtact tactggcagc ctgtggttcc aaatctagac ccgccgggga
     1021 aggaatttga tttcaagaaa tttgcaagcg aattataact cacttctgca tttgacgggc
     1081 aaagcgttca ggcaacagag caaacgcttt gcctggatgg tcgcagctag ttcgcggtca
     1141 agcgtccaaa ttcgcgcagg agatgaacca gtttccttga cgcgctaaag cgccggtaag
     1201 tatagttgcc tttcagtttc acaaccatct taaccctcct tataccaacg gatcagagac
     1261 tttcgctaca gccgatgaac gatttcagac gactcctcag gtatctaaaa cctcactggg
     1321 gaatcttcat attggcgacc attgccatgc tgatcgtcgg gctcttggaa ggcgccattg
     1381 gcgcgctaat cgtgccaatt tttaatcagg catttgccca gggagcaggt cagcgcaccg
     1441 ccactctatt tggattggaa cgaatcattc cagattctgg tctggccgcc tggaagacga
     1501 tctcgattct tctgctaacc tttaccctgg caaagggcgt tgcagagtat ttctcgacct
     1561 atctaatggc gcgcatcggc cagtctgcgg tacttcagct gcgccaggat ctttaccgcc
     1621 acgttctctt gcagtcggca agtttcttcg aaaggcatcg caccaactat ttggtttcgc
     1681 ggctcgtctc cagcgcggca gcaatcgaag cggccgtgac gagcacgatt cgcgacatgc
     1741 tgcgcgagag cttcacgtta atcgtcttcc tcggcgcgtc ttttttttat agctggcgtt
     1801 tgaccctggg ttcgctcttg ctcgcaccca tggttgcggt tctgacagca cgctttggca
     1861 cgcggctgcg aaatctggcg cgcgatagct ttgaaggtag caaagaactg actgacactg
     1921 ctcaggaggc actttccaat caaagcatcg tgaaggctta tcgcggagag gcgcgcgaag
     1981 ctgagcgttt tacaactgtg gcgcaacgca ttgtgcgcgc caacctgcga accgcacgca
     2041 tttctggttt tgctccgcca tccattgaaa tgcttggcgt ggtctttatt gtcttcctgc
     2101 tctacttcgg gcgacacgag atcgtttccg gccgcatgaa tgccgcgcag ttcttagcat
     2161 tccttttctt cctgtttcgc agctacgatc cgatgcgcaa gttgtcgcgc ttgcaaaaca
     2221 gcatggagca ggctctcgcc gcggcacatc atgtttggga agtgatggac gaacatgtgg
     2281 agattccgga gcgggcagat gcggtagaac tccagccact aaaacgcgga attgagttgc
     2341 gcgacgtttc gtttggctat gccaacgagg gtcggccagt gctacgcaac gtaagtttgc
     2401 aagtagccgc cggaaccatg gtcgcgttag tgggggagtc gggtggcggc aagtcaaccc
     2461 tgacgaaact actgcctcgc ttccacgatc cgatttccgg agcggtgttg tgggatggcg
     2521 tggatctgcg caaggtgcga cttgccagtt tgaggaaaca aatcgccttg gtaacccaag
     2581 agacggttct gttcaacgac actgtcctgc acaatatctc ttatggcaag ccagaggcga
     2641 cccaggccga agtagaagca gcagcaaaaa tagccttcgc tcacgacttc atcatggagc
     2701 tgccccacgg ataccaaacg atcgtgggcg agcgcggagt ttttctgtcg ggagggcagc
     2761 gtcaacgtct ggcaattgcg cgatcgttgc tggttgatgc gcccctgttg atcctggatg
     2821 aagcaacatc agctctcgac gctgaatccg aacgttttgt gcagcaagcg attgccaatc
     2881 tcgtgcgcaa ccgaacgaca ctagtgattg cacaccggct atcaacggtg cgccgcgcgg
     2941 acaagatcgt ggtgatggaa gccggccgga tcatcgaaac cggatcgcac gaggagttgc
     3001 ttgctcgcgg cggacagtat agaaaacttt atgagcttca gtttgctgat gaagagaatt
     3061 c
//
LOCUS       DASUEX010000481         6073 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_895212, whole genome shotgun sequence.
ACCESSION   DASUEX010000481 DASUEX010000000
VERSION     DASUEX010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6073
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_895212"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..791
                     /locus_tag="VFV61_08070"
     CDS             <1..791
                     /locus_tag="VFV61_08070"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4900472.1"
                     /translation="AVNLGMSIGPVIGGFLAMRSFKSLFYVDGTSAIIAGALIAFMPW
                     RLQEAAPNEIATGSPTKPAASQGYASVLRDRRFVYFLIAMLPVEMVFFQSLAAMPLFV
                     VRDLQISEAGFGMLLAINTVLIILTEVPLNSAMSHWSHRRTIALGALLVGVGFGGLAL
                     SNGAAAVAATVVVWTVGEMILLPASSAYVSEVAPPAQTGAYMGLYTMGFSLAFAIGPW
                     LGVQVLERIGSAGVWLGTLGCGCLTAFMIWRQRREQKSVVSSGY"
     gene            882..1313
                     /locus_tag="VFV61_08075"
     CDS             882..1313
                     /locus_tag="VFV61_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900473.1"
                     /translation="MRTFLIFSLLLLICIATCSCKDYSTGLQQGASRADETSAIATLR
                     TIAQAQTTYSISNAGHYTDFAELVEGGFLDSRFQHTNPELHGYIFTLTVNSDTSSYYC
                     NADPAPTSPHGGRHFYIESDSHDIRVNPTQPATGKDEILRP"
     gene            1509..1889
                     /locus_tag="VFV61_08080"
     CDS             1509..1889
                     /locus_tag="VFV61_08080"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX4900474.1"
                     /translation="MDKINLNEKLTLINEHWKPHIIGDLNNQQVKLVKFQGPFTWHHH
                     DHEDELFLVVKGRFRMEYREGNETTSERSIWLEPGELVIVPRGVEHRPVADEEAHVLL
                     FEPATILNTGNVENELTVHNLQRL"
     gene            2237..3373
                     /locus_tag="VFV61_08085"
     CDS             2237..3373
                     /locus_tag="VFV61_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900475.1"
                     /translation="MNRTFPLLLTATLLFAISTAAMAQTSQSIVTSAAVTSSGTAPTQ
                     DIRSILASLPEADTLVFINPRRIVNEVAPRLMWEKDLEGMRKTFADMNQFAGVDPTKM
                     DFIVIAVRFRKPTADLKFVPPEFMTVTSGDFKSESLMNLARMASEGKLRDEQYGSKTL
                     SLMTIDPIAKEAEKNPFLKSFAEVGVVALTDNSIAAGTPNYLRAAIDAANGNGRISTE
                     SLNSLLRDPTVLASAAGSPMESFSRSFGILGTETNPRAPKCDSNLGNFYVALTMDATN
                     FMLRGAMGADNPDTANIMNSLLSGLLRQAGSYVPDKSGQFVLNSLQLSAKDNEVVLSA
                     DLPQQMVRDFIRSQSQPKKQDAAMSSPATKAPAKKPVRRKRRRT"
     gene            3604..5541
                     /locus_tag="VFV61_08090"
     CDS             3604..5541
                     /locus_tag="VFV61_08090"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX4900476.1"
                     /translation="MKTLARILFIIAFTCPSLLAQNAIVPNENLVAEGIPTIPASLAD
                     VVGRYTEFRAASFSSWHPTKREMLITTRFGDTAQVHQVKFPGGDRRQLTFFPDRVGFG
                     SFPPKGGDFFVFSKDSGGDEFFQGYRYDLATGNVTMLTDGKSRNTGGSWSNSGKWLAY
                     QTTRRNGKDVDLYLVNPTDPKSDKMLLQLEGGGWGAADWSPDDRQILLFEFISANESY
                     LWLVDTTAGTKSLLTPKGGAEKVAYGGAGFSKDGKGIYVTTDLGSEFQRLAYIDLATK
                     QHKFLTDHIKWDVDEFDLSPDGKTIAFVTNEDGIARLHLLDTATGKEHPVTNLPIGQI
                     FGIDWHENGIDLGFSMVSARSTSDAYSLDVKSGKVERWTFSETGGLNTDNFSEAELVR
                     WKTFDDKMISGFLYLPPKKFTGPRPVVINIHGGPEGQARPGFNGRGNYFLNEMGVAII
                     FPNIRGSSGYGKTFLKLDNGFLREDSYKDINALLDWIKTRPELDADRIMVTGGSYGGH
                     MTLAVATNYSDRIRSALSVVGPSNLVTFLKNTQEYRRDLRRVEYGDERDPKMAEYLEK
                     TAPLNNVQKIKKPLFVVQGKNDPRVPASESEQMVAAVRKNGTPVWYLLGKDEGHGFAK
                     KKNVDYQFYATIMFMQEHLLK"
     gene            5580..>6073
                     /locus_tag="VFV61_08095"
     CDS             5580..>6073
                     /locus_tag="VFV61_08095"
                     /inference="COORDINATES: protein motif:HMM:NF022479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HEX4900477.1"
                     /translation="MDPAAAIAVLTAMITPAVLISACGSMILSTSGRLGRVVDRVRAL
                     SDRLEATATQNNAEVSRERQQIIFLQLDKLTSRARILQRAMVTFYVALGMFVATSVAI
                     GIVAIVDKPRYNMVPVIVGIIGACFLFYGSMLLIFEARMALSTIHVEMDFIWRQTTRS
                     APAD"
BASE COUNT         1453 a         1605 c         1615 g         1400 t
ORIGIN      
        1 tggccgtgaa tctgggtatg agtattggcc cggtgattgg cgggtttctg gcgatgagat
       61 cttttaagtc actcttctac gttgacggca ccagcgccat cattgccgga gctttaatag
      121 ccttcatgcc ttggcgcctt caggaagcag cgccaaacga gattgcgacc ggctcgccga
      181 caaaaccagc agcctcgcaa ggatatgcga gcgtcttacg agatcgtcgc ttcgtttatt
      241 tcctgatcgc catgttgcct gtggaaatgg tgttctttca atcgctcgca gcaatgccgt
      301 tgtttgtagt tcgcgacctc caaatctcgg aagctggctt tggaatgttg ctggccatca
      361 acaccgtgct gatcattttg acggaagtgc cactaaatag tgcgatgtct cactggtctc
      421 atcgccgaac gattgcgttg ggagctttgc tggtgggtgt gggtttcggc ggactggcgc
      481 tcagcaacgg ggctgcagcc gtggccgcaa ctgtcgtcgt ctggaccgtg ggtgaaatga
      541 tcttgttgcc ggccagctcg gcatacgttt ctgaggttgc accgccggcg cagacgggag
      601 catacatggg cctgtacacg atgggattca gtctcgcgtt cgccattgga ccgtggttgg
      661 gagttcaagt cctggaaagg atcgggtcgg cgggggtttg gttaggcacc ctcgggtgcg
      721 gttgcctcac agcattcatg atctggcggc aacggcggga gcagaagtca gtagtcagta
      781 gtggttacta gtgagtgcta atagtagtca gttggtctga ctcggcgccg agacaatcta
      841 gaatcggcaa attgaaactg gcaatgtcac ggaggatcct catgcgaacc tttttaatct
      901 tctcgctcct gctcctgatt tgtattgcga cctgtagttg taaggactac tcgaccggct
      961 tacaacaagg tgccagtcgc gctgacgaga cctcggcgat tgcgacgctg cgcaccatcg
     1021 ctcaggcgca aacgacttac agcattagca acgccggcca ctacaccgac tttgcggagt
     1081 tagtagaagg cggttttctg gactcgcgct ttcagcacac caatcccgaa ttgcacggct
     1141 acatttttac cttaacggtg aattcggata cgagttcata ttattgcaac gccgatcccg
     1201 ctccaacatc gcctcacggc ggtcggcact tctacattga gtcagactca catgacatta
     1261 gagtgaaccc gacgcaacca gcgacaggca aagatgaaat cctccggcca tgagcgccga
     1321 agcgatttca ttctcaccgc gcttcagcgc ggtgtttcgg gtggcatttg ttggaagccg
     1381 tttaaacggc ttcccttcgt aattgggaaa gcgcggcacc ggccttaagg ccggtgagaa
     1441 tgagatgtcg ttggctcttt tatccgcaat cgcttccaag ccgcgccgat gaaactcgga
     1501 gacaaacaat ggacaagatt aatctcaacg aaaagctcac actaatcaac gaacactgga
     1561 aaccgcatat catcggcgac ttgaataacc agcaggtcaa actcgtaaag tttcagggac
     1621 ctttcacctg gcatcatcac gaccacgagg atgaattgtt tctggtagtg aaaggacgat
     1681 ttcgcatgga gtatcgcgag ggcaacgaaa cgacctccga acgttcgatc tggctggaac
     1741 ccggcgagct ggttatcgtc cctcgcggcg tagaacatcg tccggttgcc gacgaagagg
     1801 cccacgtgct gctttttgaa ccggccacaa tcttgaacac cgggaatgtc gaaaacgaat
     1861 taacggtgca taacctccaa cggctgtgat cgaaatttag ccactcaccg ccaaggatgc
     1921 caagacacgc caagggcaac caagaattcc tgagacatgg aacctggtta tctgactggc
     1981 caatttatac tcggtgtctt ggcgtacctc ggcgccttgg cggtaataag gtttagaact
     2041 cgctttgttg ctgaaagaaa ctaatgacgc gagcacgttt cctgtgtgaa gatggggtgc
     2101 cttaatttgc taccggggcg gcactccgtg gccgccccac ttcttgaaaa atggggctcc
     2161 cacggaggac gcccctacaa atttctaaga agatactgcc gaacatggct gaaattttga
     2221 aatggaggct tttgccgtga atcgaacttt tcccctgcta ctcactgcca cgttgctgtt
     2281 cgcgatctca acagcagcga tggcccaaac ttctcaaagc atcgttacca gtgcggcagt
     2341 aacctcatct gggactgctc cgacgcagga cattcgcagc attctcgcga gtctcccgga
     2401 agcagacacg ctcgttttca ttaatccgcg ccgcatcgtc aacgaagtcg ccccgcggct
     2461 gatgtgggaa aaagatctcg aaggaatgcg caagacgttc gctgacatga atcagtttgc
     2521 cggagtcgat ccgacaaaaa tggacttcat cgtcattgca gttcgttttc gcaagcccac
     2581 tgccgatctc aagttcgtgc cgcctgagtt tatgaccgtt acgagcggtg actttaagtc
     2641 tgaatcactc atgaacctgg cgcgaatggc ttcagaagga aagttgcgcg atgagcaata
     2701 tggatcgaaa acactttcgc tgatgaccat cgaccctatt gccaaagaag cggagaagaa
     2761 cccttttctc aagtcgtttg cggaagtcgg agtggtagcg ctgacggaca actccattgc
     2821 cgcgggtacg cccaattatt tacgcgcggc aatcgatgcc gccaacggca acggtcgcat
     2881 cagtaccgag agtcttaact ccctgctgcg cgatcctact gtcctcgcga gcgctgccgg
     2941 ttcgcccatg gagtcgttct ccaggagttt tggcatcctc gggacggaaa cgaatccgcg
     3001 tgcgccaaag tgcgattcca acctgggcaa cttctatgtg gcactcacaa tggacgccac
     3061 gaacttcatg ttgcgcggtg cgatgggcgc agacaatcct gacacggcaa atatcatgaa
     3121 cagcctcctg tccggactcc tgcgtcaggc tggctcttac gtgccagaca agtccggcca
     3181 gtttgtgctg aacagcttgc agcttagcgc caaagacaac gaagtagtat tgagcgccga
     3241 tctgccgcag caaatggtgc gcgacttcat caggtctcag agtcagccga agaaacagga
     3301 cgcagctatg tcttctcctg cgactaaggc acctgcgaag aagcccgtcc ggcgcaagcg
     3361 ccgacgaacg tagagacatg gccagttgca gaacatagca gacgactgag agcaaatcag
     3421 caatctacgg ccgggttaga gcaacctaaa agtccattag tccgcgcaac gaacggccga
     3481 aaggaacctc atcagtttcg gtggttcgct tcgcgggctg tcagaaactg gcaattggca
     3541 atatagtatc cctccccaga ccaaccgatt ctttttccgc tgcgttgctg aaggagattc
     3601 aacatgaaaa ccctggctcg aattctgttc atcattgcct tcacttgccc gtctcttctg
     3661 gcgcaaaacg ccatagtccc gaatgagaac ctggtcgctg aaggaatccc gaccataccg
     3721 gcctcactcg cagacgtggt tggtcgttac accgagtttc gcgcggcatc tttctcgagc
     3781 tggcacccga ccaaacgcga aatgttgatt acgactcgct ttggcgatac agctcaggtt
     3841 catcaggtta agtttcccgg tggagatcgt cgacaactga cctttttccc cgatcgcgtc
     3901 ggcttcggaa gcttcccacc gaagggtggc gactttttcg tctttagcaa agacagtggt
     3961 ggtgatgagt ttttccaggg ctaccgttac gatctggcaa ctggaaacgt cacgatgctt
     4021 actgacggca aatcccgaaa caccggcggc tcctggtcaa attccggtaa atggctcgca
     4081 tatcaaacga caaggcgtaa cgggaaagac gtcgatctat atctcgtgaa tccgaccgat
     4141 cccaagtccg acaagatgtt gttgcaacta gaaggtggcg gctggggagc ggcggactgg
     4201 tctcccgatg atcgtcaaat actcctcttc gaattcattt cggcgaatga aagctacctc
     4261 tggctcgttg acacgactgc aggtactaag agcctcttaa cgcccaaggg tggtgcagaa
     4321 aaagtcgcat atggcggcgc tgggtttagt aaggatggca aaggcattta cgtcaccacc
     4381 gatctcggct cggaatttca acgcctcgct tacatcgacc tcgcaaccaa gcaacataag
     4441 tttctgaccg atcacatcaa atgggacgtc gacgaattcg acctctcacc tgacggtaaa
     4501 accatcgcct ttgtaactaa cgaagacggt attgctcgtc tgcacttgtt ggacacagca
     4561 acaggaaaag agcatcccgt taccaatcta cctattggtc agatcttcgg aatcgactgg
     4621 catgagaatg gtatcgacct gggcttcagt atggtgtcag cgcgttcgac ctccgacgcc
     4681 tattcgctcg atgtaaaaag cggaaaggta gagcgatgga cgttcagtga gacaggagga
     4741 ctcaacactg ataatttttc agaagctgaa ctggtccgtt ggaaaacctt tgacgacaag
     4801 atgatttccg gttttcttta tctgccgccc aagaaattta ccggcccgcg gcctgttgtc
     4861 atcaacatcc acggcggacc cgaaggacag gctcgtccgg gatttaacgg acgcggcaac
     4921 tatttcctaa atgagatggg cgtcgcgatt atctttccga acattcgcgg ttcgtcagga
     4981 tacggcaaga cctttctaaa actcgacaac ggatttttac gcgaggattc ctacaaggac
     5041 attaatgcct tgcttgactg gatcaagacc agacctgagc ttgatgcaga ccgaatcatg
     5101 gtgactggag gctcttatgg cggtcacatg acgctcgcgg tagcgacaaa ctacagcgat
     5161 cgcattcgct ctgctctctc agttgtggga ccgtcaaatc tggttacctt cttgaagaac
     5221 acacaggaat accgacgcga cctgcgccga gttgaatatg gggatgagcg tgacccgaaa
     5281 atggcggagt atctcgaaaa aacggcgccg ctgaacaacg tccaaaaaat caagaagccg
     5341 ctgttcgttg ttcaggggaa aaatgatcct cgcgtcccgg ccagtgaatc ggaacagatg
     5401 gtagcggcgg tgaggaaaaa tggaacgcct gtctggtatc tactgggcaa ggacgaaggt
     5461 cacgggttcg ccaaaaagaa aaatgtcgac tatcagttct acgcgacaat catgttcatg
     5521 caggaacatc tgttgaagta gtgcgacgac ttttctgcag atgagttaat accgatttaa
     5581 tggatcccgc agcagcaatt gccgtcctga cggcgatgat tacaccggca gtgttaatct
     5641 cggcttgtgg cagcatgatc ctctccacct ccgggcgatt aggtcgcgtt gtggatcgcg
     5701 ttcgcgcact ttctgatcgt ttggaagcga cagcgaccca gaacaatgcg gaagtatcac
     5761 gcgaacgcca gcaaatcatc ttcttgcaac tggacaaact aacgagtcgc gcgcgcatac
     5821 tgcaacgcgc gatggtcact ttctacgtgg cattggggat gtttgtcgcc acgagtgtgg
     5881 cgattgggat cgtcgcgatt gtcgataagc cgcgctataa catggtaccc gtcattgtgg
     5941 gaatcatcgg cgcttgcttt ttattctatg gcagcatgct tctgatcttc gaagcgcgta
     6001 tggcactcag tacgattcac gtggagatgg atttcatttg gcggcaaaca acacgcagcg
     6061 ccccggctga tat
//
LOCUS       DASUEX010000482         4851 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1336669, whole genome shotgun sequence.
ACCESSION   DASUEX010000482 DASUEX010000000
VERSION     DASUEX010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4851
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1336669"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="VFV61_08100"
     CDS             <1..588
                     /locus_tag="VFV61_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900478.1"
                     /translation="DETGTLCMTLQAAVDFDQFVRCYCVGQQEVMIMAYDPRKPYLSG
                     EQYIHHPDYLEPALKDRVTKDVRTLCTALGYDLNTVEFAIKDGVPYAIDFMNPAPDAE
                     LASVGEYYHNWIVGAVADLIFKRLAEPRQRPRYRWDALLNPEPQSTAAVASPAEQQAR
                     TIVPTPATTEAPKAKTATAATARKKSSPSRSKPTA"
     gene            complement(676..2097)
                     /locus_tag="VFV61_08105"
     CDS             complement(676..2097)
                     /locus_tag="VFV61_08105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900479.1"
                     /translation="MNETNQIEALQSLAEVAKVRKQSGKRTYLATVRVSPGAYLAVSS
                     VLTFVAALLLRSGSEGWALILVLLAWALVPALALTDRIAFDGEVLIRRGLIPFLRHFF
                     SGKREQLSIADVERVDTNAVRTLRRGGRVRYRYRSQLFGKGTGFAFASGGPRYRDMVR
                     QLFPVIVDNKLDLRTRDLRDYLCDPKRLSQEAKTLQLASPDVLDNATSDLKVGRRGEA
                     AETRAEVTGAAVERAAHLRQLGNRLRIAGRLKEAREAFRRALIVLPHDAWLIFDFARL
                     LRSQASAEADADLLARARAALRLSSMRADEDARLLALLGESLLEYNDVTRAERNFQRA
                     LELEPGTYRARLGLADVALRSGKLAHVINQYRDAAVATNEKALVAFANREADYYFSLN
                     DDDDYLSSELRRINWLQTLSHMRRLAARITNASILIALLGPYLNSTIGSMGWALASSS
                     LVAWLLGMFGTRLLAERRKRPTE"
     gene            complement(2121..3254)
                     /locus_tag="VFV61_08110"
     CDS             complement(2121..3254)
                     /locus_tag="VFV61_08110"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEX4900480.1"
                     /translation="MDNLTHSLLGLAAAKAGCERLSPGATALCLVAANAPDADVAVLL
                     FADRWSFLHYHRHITHSIIGTLAIALLLPLIFWVGDRGLARLRSRPPVVRLQGLMLVS
                     LIVSATHPLLDWTNNYGVRLLLPWSSKWFYGDLVFIVDPFIWLLLGSAAFLLTARRKW
                     QVIGWSIIASVLSYLVLTTSAERGLAHATQLKVLWVGVITALVLAHRLGAAQRWGPRL
                     AQTAFGLLVVYWIGLGVMHSMALREVNLAAASIAKDRGETVTDFAAMPTLANPFRWLA
                     VAETNRAAYRFEVSLTDHFSYPAAPVRHERADTMGSPFLLSAAKDRRARIFLGFARFP
                     VYRLSGVDCLSETIVQLADLRYTQPGSTRGTFALEVPVTCPPE"
     gene            complement(3292..4098)
                     /locus_tag="VFV61_08115"
     CDS             complement(3292..4098)
                     /locus_tag="VFV61_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900481.1"
                     /translation="MARYKKKRARELQHDRFRDTTIGIFDRLGDLLEGKGRTILYVIG
                     GVIVAAALVGGWLTWSRRKTDEARRAMGRAIAIAATPVNATPIPGTSAPSFPSEQERA
                     RKAIEEFEKVAAKYGDPYHTKARYFVAANQLYIEREKGINELTQLSSSGMPDVAILAK
                     FALAQAREVDAKYDEAASLYSQIATLNSVIVSPETANLRLAMVYQKQGKTKEATDTLF
                     QIADNARKAKDKDGAPVPLSEAARDAAQQLQKLDPERFTQLTPEAPALNL"
     gene            4353..>4851
                     /locus_tag="VFV61_08120"
     CDS             4353..>4851
                     /locus_tag="VFV61_08120"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX4900482.1"
                     /translation="MSFGEPIALRGQMDLEQVAAATPGEVRSPLETQFIERLKQGEAA
                     AFEVLIAERSGEVYGLLYRLTENSEEARDLTQETFLRAFQSIGQFRGDSDLRTWIYRI
                     AINQARNRWRWWRRRRRDATVSLDSENAHGNVPLSESLRSEREKSPEQQTLAHERERA
                     LRRALQ"
BASE COUNT         1126 a         1371 c         1366 g          988 t
ORIGIN      
        1 gatgagacgg gcacgctttg catgaccctc caggcggcag ttgatttcga tcaattcgtt
       61 cgttgttatt gcgtgggcca acaggaagtg atgatcatgg cctatgatcc gcgcaagcct
      121 tacctttccg gcgaacagta cattcaccat ccggattacc tggagcccgc cctgaaggat
      181 cgcgtcacca aagatgtgcg aactctctgc acggcccttg gctatgatct caacaccgtc
      241 gagtttgcga tcaaggacgg cgttccttat gcgatcgact ttatgaatcc ggcgccggat
      301 gccgagctgg cttcggttgg tgagtactat cacaattgga tcgtcggcgc tgtggcagac
      361 ttaatcttta agcgactggc tgaaccacgg cagcggccac gttatcgttg ggatgccttg
      421 ctgaatccgg aaccgcaatc taccgcagcg gtagcttccc ccgctgagca gcaagcgcgt
      481 acgattgttc caactccagc cacgacggag gcacctaaag cgaaaacggc gacagccgct
      541 accgcgcgga agaaatccag ccccagtcgt tcaaagccga cggcttaaac aaaaaaaccc
      601 aaacccgcga aggcggactc gggtgctcat aagttaagga cagggattcg ttcctgtcct
      661 ttttctttgt cgaaactatt cggtcggacg tttgcggcgt tcagccagaa ggcgtgttcc
      721 aaacataccc agcagccagg ctacgagtga cgatgaagcc agcgcccacc ccatgctgcc
      781 gattgttgag ttcaagtaag gacctaacaa ggcgatcaag atgctggcgt ttgtaatccg
      841 agctgccagg cgccgcatgt gcgacagggt ttgcagccag ttgatccttc ttaattccga
      901 agataagtaa tcatcatcgt cgttaagaga aaagtagtaa tccgcttcgc ggtttgcaaa
      961 cgccaccaga gccttttcat tggtagcgac tgcggcatcg cgatattgat tgatcacatg
     1021 agccagtttg ccgctccgca acgccacatc ggccagccct agccgcgctc gatacgtgcc
     1081 cggctcaagc tcaagggcgc gctgaaaatt tcgttcggca cgcgtgacat cgttgtattc
     1141 aagtaagctc tcgcctagta atgccagtag cctggcatct tcatccgcgc gcatcgagga
     1201 aagacgcagc gccgctcgcg cccgcgccaa caaatcagcg tctgcctctg cgctggcctg
     1261 cgagcgcagc aaacgtgcga agtcaaaaat caaccaggca tcgtgtggca gaacgataag
     1321 tgcgcgccga aaagcctcgc gcgcctcctt cagtcgtccg gcaatcctta acctgtttcc
     1381 cagttgtctt aagtgcgccg ccctttcgac agcggcccct gtaacttcag cgcgcgtctc
     1441 tgcagcttcg ccgcgtctcc caaccttcaa atcgctagtc gcgttatcca aaacatccgg
     1501 cgaagcgagc tgcaatgtct tggcttcctg actcaaccgc tttggatcgc acagatagtc
     1561 gcgcaaatcg cgagtacgca gatctaactt gttgtcgaca atcacaggaa acagttgccg
     1621 caccatgtcg cgataacgcg ggccgccgga agcaaatgca aacccggtcc ctttcccaaa
     1681 aagttgactg cgatagcgat aacgcacccg tccgccacgg cgcagcgtgc ggaccgcatt
     1741 ggtatccacg cgttcgacgt cggcgatact aagttgctca cgcttcccgg aaaagaagtg
     1801 ccgcagaaaa ggtatcaggc cacggcggat caaaacttcc ccatcaaacg cgatgcggtc
     1861 ggtgagagcc agcgccggca ccagggccca ggcaagtaag accaggatca aagcccagcc
     1921 ttcgctcccg gagcgcaaca acagagcggc tacgaacgtc aggacacttg agaccgcaag
     1981 gtaggcccca ggtgagaccc gcacagtcgc caaataggtt cgcttgccag actgctttcg
     2041 gaccttggca acctcggcca gggactgcaa cgcttcgatt tgattagttt cgttcactgc
     2101 tagctgccgt tcttaactct ctactcaggg ggacacgtaa cgggaacctc cagcgcaaaa
     2161 gttccacggg tagatccggg ttgagtgtaa cgtaagtcag ccaattgcac gatggtttca
     2221 ctcaagcaat caacgcctga cagcctataa acgggaaaac gggcaaagcc aaggaaaatc
     2281 cgcgcgcgcc gatcttttgc agcgctcaga aggaaaggcg aacccatggt atcggcgcgc
     2341 tcgtgacgca cgggcgcggc ggggtagcta aagtggtcag taagggatac ttcaaatcga
     2401 taagcggcgc gattagtttc cgccactgcg agccagcgaa aaggattggc cagcgtcggc
     2461 atcgccgcaa agtcggtaac agtctcgcct ctgtccttcg cgatactcgc tgccgcaagg
     2521 ttaacttccc gcagggccat tgaatgcatt acgccgagac cgatccaata aacaacgagt
     2581 aacccgaacg ctgtttgcgc cagccgcgga ccccaacgct gagccgcgcc caggcgatgc
     2641 gccaaaacaa gtgcagtgat aactcctacc cagagaacct tcagttgtgt tgcatgtgct
     2701 aaaccgcgtt cggcagacgt tgtcagcacc agatagctaa gaacgcttgc gatgattgac
     2761 cagccgataa cttgccattt gcgcctggca gtcagcagaa aggcggcgct cccgagcagt
     2821 aaccagataa aaggatcgac aatgaagacg agatcgccgt agaaccactt gctgctccag
     2881 ggaagcaaca gccgcacgcc atagttgttc gtccaatcga ggagcggatg agtcgcacta
     2941 acaatcagcg agaccaacat cagtccctgg aggcgaacga ctggaggacg cgagcgcaac
     3001 ctggcgagcc cgcgatcgcc tacccagaaa atcagcggca gcagcaaagc gattgcgagg
     3061 gtaccgatga ttgaatgggt gatgtgccgg tgatagtgaa gaaaagacca gcggtccgcg
     3121 aaaagcaaga cggcgacatc agcgtcaggc gcgttagctg cgacaagaca aagggcagtc
     3181 gcgcccggtg acaatcgttc acagcccgct ttggctgctg ccagtcccag aagcgagtga
     3241 gtcaggttgt ccatcgatgg tctggatgcc gacggaggga attgcagtcg gtcagagatt
     3301 cagtgccggc gcttcgggcg ttagctgagt gaaccgttcc ggatccagtt tttgcagttg
     3361 ttgcgcagcg tcgcgggcgg cctccgaaag ggggacgggc gctccgtctt tgtcttttgc
     3421 tttacgcgcg ttgtcggcga tctggaaaag ggtgtccgtg gcttccttcg tctttccttg
     3481 tttttggtaa accatcgcca gacgcaagtt tgccgtttct ggtgagacaa tcacgctgtt
     3541 gagggtcgcg atttgggagt aaagagatgc tgcctcatcg tacttagcgt caacttcccg
     3601 agcttgagcg agggcaaatt ttgcaagaat ggccacatcc ggcataccgc tggaactcaa
     3661 ctgcgtcagc tcattgatgc ccttctcgcg ttcgatgtaa agctgattcg cggcgacgaa
     3721 ataccgtgct ttggtatggt acgggtcacc gtatttggca gccaccttct caaactcctc
     3781 gattgctttt cgcgcccgtt cctgttcact gggaaatgaa ggtgcgctgg ttcccgggat
     3841 gggagtggcg tttaccgggg tggcagcaat ggcaatcgct cttcccatcg cgcgacgtgc
     3901 ctcatcagtc ttacgacggc tccaggtaag ccagccacca acgagggccg cggcgacaat
     3961 aacgccgcca atgacgtaca ggatcgttcg ccccttgcct tccagcaggt cgcccaaacg
     4021 gtcgaaaatg ccgattgtcg tatcgcgaaa acgatcatgc tgcagttcgc gagcgcgttt
     4081 ctttttgtat cgagccacgt gtttccttct tgcaagtccc cataggggcc agcgttcttc
     4141 aaggtgtatg ataagcgaaa ccagcactct aatggtcggt ccgaaacatt gtcaagcggg
     4201 tgttttgttc gctactggcc gtgcttgcat ctaagaagtc gaatgaaaat ctttagggaa
     4261 caacacgagt accagtggtg tctaaggtgt cgcctgtccg ggaatggacg tgggcggtcc
     4321 gcaactccga ttcccgcccc agggggtaag cagtgagctt cggagagcca attgccctcc
     4381 gcgggcagat ggatttagag caggttgccg cggcgacccc aggagaagtg cggtcgccgc
     4441 tcgagacaca gttcattgaa agattgaagc aaggcgaagc ggcagctttc gaggtgctca
     4501 tagccgaacg ctcgggtgaa gtttacggac tgctttatcg acttactgaa aacagcgaag
     4561 aagcccggga cttgactcag gaaacatttc tgcgagcgtt tcagagcatt ggacagtttc
     4621 gcggtgattc cgacttgcgg acctggatct accggatcgc aatcaatcag gcgcggaaca
     4681 ggtggcgctg gtggcgaaga cggcgacgtg atgcgaccgt ctcattggat tctgaaaatg
     4741 cgcatggcaa tgtaccgctt agcgaatcgc tccgatcgga acgcgagaag tcgccagagc
     4801 agcagacgtt ggcgcacgaa cgagagcggg ccctgcgccg agcgctacag a
//
LOCUS       DASUEX010000483         3291 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1221888, whole genome shotgun sequence.
ACCESSION   DASUEX010000483 DASUEX010000000
VERSION     DASUEX010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3291
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1221888"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="VFV61_08125"
     CDS             <1..241
                     /locus_tag="VFV61_08125"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX4900483.1"
                     /translation="EGNTPTGESFHVKAQAIKISRGGATIVLDREIAVGSVVTLTPPF
                     GKKLEAEVNGVWKDEIDSKQRIGVKLLDTDGWFAE"
     gene            266..1981
                     /gene="recJ"
                     /locus_tag="VFV61_08130"
     CDS             266..1981
                     /gene="recJ"
                     /locus_tag="VFV61_08130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEX4900484.1"
                     /translation="MNKRWIVRAHDASRAASLARVLNVSPIVAALLLARGCDDESSAR
                     AFLKPSYEQLHDPYLMLGMKEAVARLQRAVDQQEKILIYGDYDVDGTTGTAVLLRALK
                     LLGADAGFHVPHRFTEGYGIQQPALEKAVAEGYSLAISVDCGIRAHEPLHWARANGLD
                     VIVTDHHLPDEGEGVPPAFAVLNPNQRGCAYPDKNLAGVGVAFKLVHALYRERDREHL
                     VPAFLKVVAIGTVADVAQLTGENRTIVSLGLQDLRNSTNPGLRALLAVAGCGEGEGMT
                     SYDLGFRIGPRINAAGRIDAARAVVELFDTRDRDEARRLAEHLEARNQERREVQQQIF
                     ELAIAELEGSQTAGPAHVAVIAGEGWHRGVIGIAASKIAERINRPCVVLSIDGEVAHG
                     SGRSIEPYHLLNGLTSCADLFEKFGGHSHAAGITIRADRIPEFRRRLNEHAASRLTVD
                     DLQPCLQIEAKLPAEAITFQLANELSSLEPFGAGNPRPIFMTSNLRRVGEPRVLKDRH
                     LKMHVIGSGGRPLETIWWNCIDEKKRTPDVKSGIELAYTIETNTWNGEVRMQLNVEDM
                     RITEG"
     gene            2147..>3291
                     /gene="lptC"
                     /locus_tag="VFV61_08135"
     CDS             2147..>3291
                     /gene="lptC"
                     /locus_tag="VFV61_08135"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HEX4900485.1"
                     /translation="MQAVTRKREIRVGMRARVPLIARALAILILTAGVAFIAISYYRL
                     RNNQPFRMRSEAPELSKEITGITNGYEQRVTKDDRLYLLLKASRDITYSDGHHELETV
                     SLAVYPPAGDKPDQISANRAIYDQANSMITFQGSVKIETKDALKVDTESLVYNQTSEV
                     GQTDAPISFSRENVSGNSTGAIVDGKSKKLELKRDVQITVAPEAKQTASASGGSRSRP
                     VSIRSARATFEHQSLRLSFYGGVTADQERDTMSGNELFATLNDQKRLQKVEVRGNSYL
                     SSREEGRGAEVQAADMDFFLDKDQRLERAVAMTDARSRTLDADADMTLTGANLLEVKF
                     QAQGERSLLREMRSEGRAVITLAAPKSKAGDARAANKRLTADAVKLS"
BASE COUNT          772 a          863 c          956 g          700 t
ORIGIN      
        1 tgaaggcaac acaccgactg gtgaatcatt ccacgtcaaa gctcaggcaa taaaaattag
       61 tcgcggcggc gccacgatag tcctcgatcg cgagatagcc gtcggatctg tcgtcactct
      121 gacccctcct tttggcaaga aactggaagc ggaggtcaac ggcgtttgga aagacgaaat
      181 tgatagcaag caacgcatcg gtgtgaaact gctcgacacg gacggatggt ttgcagaata
      241 gcgagagata aactaatgtc cgcttgtgaa caaacgctgg atcgtccgcg cgcatgatgc
      301 tagccgcgct gcctcacttg ctcgcgtttt aaacgtctca ccaattgtcg ccgccctgct
      361 cctcgcccgc gggtgcgacg atgagtcttc cgccagagct tttctcaaac caagctacga
      421 acagcttcat gatccctact tgatgttggg gatgaaggag gcggtggcac gtctgcaacg
      481 agccgttgat cagcaagaga agatccttat ctatggcgac tacgacgttg atggcacgac
      541 cgggaccgcg gttttgcttc gggcgttgaa gcttctgggt gcggacgctg gttttcatgt
      601 gccgcatcgt ttcaccgaag gctatggcat ccagcagccg gcccttgaga aagccgtggc
      661 tgaaggatat tcactcgcta tcagcgtgga ctgcggcatc cgcgcacacg agcccctgca
      721 ctgggcccgc gctaatggac tcgacgtcat tgtcacggat caccacctgc ctgatgaagg
      781 cgaaggggta ccgcctgctt tcgctgtcct caatcccaac cagcgaggct gcgcttatcc
      841 cgacaagaat ctcgcgggtg tgggggtggc cttcaagttg gttcatgccc tctatcgcga
      901 gcgcgatcgc gaacatctgg ttccggcctt cctgaaggtc gttgcgatag gaacggtggc
      961 tgatgtggcg cagcttacag gagagaatcg cactatcgtt tcactcggtt tgcaggatct
     1021 gcgcaattcc acaaatcccg gtttgcgagc gttgttggca gtagcgggtt gtggcgaggg
     1081 tgagggaatg acttcttatg acctgggttt tcggataggt ccgcgcatca atgccgccgg
     1141 aaggattgat gcggcgcgtg cggtagtgga actcttcgac accagagatc gagacgaggc
     1201 ccgccggctg gctgaacatc tcgaggcacg taatcaagag cggcgcgaag tgcagcaaca
     1261 gatctttgaa ctggcgattg ctgagttaga agggagccaa acggcaggcc ccgcacatgt
     1321 agcagtgatc gctggagaag ggtggcatcg gggcgtgatt ggcattgccg cctcaaagat
     1381 tgccgaacgg atcaatcgtc cttgcgttgt gttgtcgatt gacggcgaag tcgcgcacgg
     1441 atcgggccgc agcattgagc cttatcatct gctcaatggc ctaacttcat gtgccgacct
     1501 gttcgagaag tttggcggcc actcgcatgc agccggcatc acaattcgtg ctgaccgcat
     1561 tccggaattc cgccgcagac tcaatgagca cgcagccagt cgtcttaccg ttgacgattt
     1621 gcagccatgt ttgcagattg aggccaagct gccggccgaa gcgatcacgt tccagttggc
     1681 aaatgagtta agctcgctag agccctttgg tgcgggcaac ccgcggccga tatttatgac
     1741 cagtaatctg cggcgagttg gcgaaccccg agtcttgaag gatcggcacc taaagatgca
     1801 cgtaattggc tctggcggcc gccccctgga aacgatctgg tggaactgta ttgatgaaaa
     1861 gaagcgaacg cccgatgtta aaagcggcat cgaactggct tacacaattg agacaaatac
     1921 ctggaatggc gaagtgcgga tgcagttgaa tgttgaagac atgaggatca ccgaaggtta
     1981 gtgattttga actttgttct ttgtacttag ctctttgatg tgcccttgaa acagatgctt
     2041 cttccggcga agagtttaag gtccggaact aaaacaaagt acaaagatca aagcactaag
     2101 aacaaaaccg aaaggattaa gagaaaaagt tgaattccaa ataacgatgc aagcagtcac
     2161 tagaaaacgg gagattcgag ttggcatgcg cgccagggtc ccgctcatcg cgcgtgcgct
     2221 cgccatcttg atcctgacag ccggcgtggc ctttatcgcc atttcttact accggttgcg
     2281 aaacaaccaa ccatttcgca tgcgctccga agcgcctgaa ctctcgaaag agatcaccgg
     2341 gatcaccaac gggtatgagc aacgagtgac aaaagacgat cgtttgtatt tgttgctcaa
     2401 ggcctcgcgc gacattactt actccgacgg acatcacgaa ctcgagacgg ttagtctcgc
     2461 ggtttatcct ccggcgggtg acaaacctga tcagatttct gcgaatcgcg cgatttatga
     2521 tcaggcgaat tcgatgatca cgtttcaggg cagcgtgaag atcgaaacga aggacgcgct
     2581 gaaagttgat actgagtcac tggtttacaa ccaaaccagc gaggtgggcc agaccgacgc
     2641 tcccattagt tttagccgcg agaacgtctc gggaaactcc acgggggcga tcgttgacgg
     2701 caaaagcaag aagctggaat tgaagcgaga cgtccagatc accgttgctc ccgaagcaaa
     2761 gcagacagca tccgcctccg gtgggtcgcg ttctcgtccg gtatcaattc gttccgcccg
     2821 cgctaccttt gaacatcagt ctctcaggct ctcattttac ggaggtgtta ctgctgatca
     2881 ggaacgcgac accatgagcg gcaatgagct gttcgccacc ttgaacgatc agaagcggct
     2941 gcagaaagtt gaagtgcgcg ggaactccta cttgagttca cgcgaagagg gccgcggggc
     3001 tgaagtgcaa gccgcggaca tggatttctt ccttgataaa gatcaacgcc tcgaacgggc
     3061 cgtggcgatg acagacgcgc gctcgcggac actcgatgcg gacgcggaca tgacgctgac
     3121 aggcgcgaat ctgcttgagg ttaagtttca ggcccagggt gaacgcagtc tgttgcgcga
     3181 gatgcgcagc gaggggcgcg cggtgattac gcttgccgca cccaagtcca aggccggcga
     3241 tgcgcgggct gcaaacaaac gtctaaccgc cgatgcggtg aagctcagtt g
//
LOCUS       DASUEX010000484         2012 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1004295, whole genome shotgun sequence.
ACCESSION   DASUEX010000484 DASUEX010000000
VERSION     DASUEX010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1004295"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="VFV61_08140"
     CDS             <1..352
                     /locus_tag="VFV61_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900486.1"
                     /translation="ATGNNQRVYLKIAESLAGRIAKERMPWADPLASLVRAGVAYKRG
                     ETAAATTTLADAAEHFDLAEMQLYAAAARRRLGKLIGGRRGEELLTEADAWMREQKID
                     NPERMTRMLAPGFD"
     gene            414..1316
                     /locus_tag="VFV61_08145"
     CDS             414..1316
                     /locus_tag="VFV61_08145"
                     /inference="COORDINATES: protein motif:HMM:NF006128.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HEX4900487.1"
                     /translation="MDTLKTVTYDVTGRIARITLNRPERGNGITLEMPAEIAACVERA
                     NLDPAVRVIAVAGNGKGFCGGYDLVLAAEQRIGGELISWPSGSPMDPQVQLDNHDPEK
                     TWDPTIDYAMMSRNVRGFMSLFYSDKPVICKVHGFCVAGGTDLALCADLLLIEDTASI
                     GYPPARVWGVPTTALWALRIGPEKAKRLLFTGDCLTGKQAVEWGLASDSAPAGELDQR
                     FESLLERVAQLPINQLIMMKLLVNQAILGQGLQAAQLLGTLFDGVARHTPEGYAFQQR
                     AGEVGFKQAVEERDRPFTDKSDKL"
     gene            1352..>2012
                     /locus_tag="VFV61_08150"
     CDS             1352..>2012
                     /locus_tag="VFV61_08150"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX4900488.1"
                     /translation="MIFRSPHAPITIPDISLPDYVLANAEEHGDKPALIDGPSGRVIT
                     YAQLRPMIRRLAAGFSSYGVKQNDVVAILSPNLPEYVLAFHAIASLGAITTMVPPLFT
                     DEEIGRQLRDSGARYLLTIPPLIARAREAGRGSNVSRTFVIGEAENAVSFASLLNTTE
                     DAPPVRIERNADLAALPYSSGTTGFPKGVMLSHRNLVAMLCQLNANEPLTEKDTMICV
                     VP"
BASE COUNT          460 a          548 c          573 g          431 t
ORIGIN      
        1 cgcaaccggc aacaaccagc gagtgtactt gaagattgct gagtcgttgg cgggacgcat
       61 cgctaaagaa cgaatgccct gggccgatcc gcttgcgagt ttggtgcgcg cgggcgtggc
      121 gtataagcgc ggagagacgg ccgccgcgac cactactctt gctgatgcgg cagaacattt
      181 cgatctggcg gaaatgcaac tctatgccgc cgccgcccgc aggcgactcg gcaagttaat
      241 cgggggcaga cgaggggaag agttattaac tgaggcagat gcatggatga gggagcagaa
      301 gatcgacaac ccggagcgga tgactcggat gctggcgccc ggcttcgatt agcagcacaa
      361 aacaatttgg actcagaact cgaaactcag aactcgaaac ccgaaactcc accatggata
      421 cacttaagac tgtcacgtac gacgttactg gccgcatcgc acgtatcacc ttgaaccgcc
      481 ccgaacgcgg caacggcatt acgttagaga tgcctgccga gatcgctgcg tgcgttgagc
      541 gcgctaatct cgatccggcg gtgcgagtaa tcgccgtggc cggaaacggg aaaggttttt
      601 gtggaggtta tgacctggtg ctggcggctg aacagagaat cggcggcgag ctgatcagtt
      661 ggccgtcggg atcaccgatg gatccacagg ttcagttgga caaccacgat cccgaaaaaa
      721 cgtgggatcc cacaatcgat tacgccatga tgagccggaa cgtacgcggc tttatgagcc
      781 ttttctattc cgataagcca gttatctgta aagtgcacgg cttctgtgtt gccggtggaa
      841 ccgaccttgc gctctgcgcg gatcttctgt tgattgagga cacagcttct attgggtatc
      901 cacctgcacg cgtctggggt gtaccgacga ccgccctttg ggcgcttcgc attggtcccg
      961 aaaaagcgaa gcgacttctc ttcaccggag attgtctgac cgggaagcag gcggtggagt
     1021 ggggcctggc tagcgactcc gcgccggcag gggaattgga ccaacgattc gaaagcctgc
     1081 ttgaacgcgt ggcgcagctt cccataaatc aattaatcat gatgaagttg cttgttaacc
     1141 aggccattct gggtcagggc ctacaagcag cgcagctact cggtacgctg tttgacggcg
     1201 ttgcccgtca cacacctgaa ggctatgcat ttcaacaacg cgccggcgaa gttgggttca
     1261 aacaggctgt cgaggaacgc gacagaccat tcactgataa gtctgacaaa ctttagttgc
     1321 tgattaatag ttctttccgg agatccccaa aatgattttc aggagtcctc acgccccaat
     1381 caccatccct gacatttccc ttccggatta cgtactcgcc aacgcagagg agcatggcga
     1441 caaacccgcg ctcatcgatg gaccttccgg gcgggtgatt acctatgcgc aacttcggcc
     1501 gatgattcgg cgtttggctg ccggcttctc gagttatggc gttaagcaaa acgatgtcgt
     1561 ggcgattctc agtccaaatc ttccggagta cgtactcgcc tttcatgcga tagcgagcct
     1621 cggagcaata actacgatgg tgcccccact ctttactgac gaagagattg gccggcaact
     1681 gagagactcg ggcgcgcgct atctgctgac tattccaccc ttgattgcca gagcacgcga
     1741 agcgggccgg ggctcaaacg tgagcagaac ctttgtcatc ggcgaggctg agaatgcagt
     1801 ttcctttgcg tcacttctga ataccacgga ggatgctccc cctgttcgaa ttgaacgcaa
     1861 tgctgatctg gcggccctgc cttattccag cggcactacc ggatttccca aaggcgtgat
     1921 gctcagccac agaaacctgg tagcgatgct ttgtcaattg aatgccaatg agccgttgac
     1981 tgagaaagac acgatgatct gcgtggtgcc ca
//
LOCUS       DASUEX010000485         2436 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_766756, whole genome shotgun sequence.
ACCESSION   DASUEX010000485 DASUEX010000000
VERSION     DASUEX010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_766756"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2073)
                     /locus_tag="VFV61_08155"
     CDS             complement(<1..2073)
                     /locus_tag="VFV61_08155"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX4900489.1"
                     /translation="MRSTIVKTLMLALLILLPTLASAQTHRATVRGTIFDPRQAVIPG
                     AELKLTNRQTNKERTAMSGPQGEYSLTSLEPGTYLLEVTFPNFQKSGQEVVLKVDQEL
                     RLDVTLFVGAVTHEGMFIDEPSELKKDSSSIGTVIENRQVSGLPLDGRNFYELTLLVP
                     GAVPAAPGSAGSVRGDFAFNVNGAREDSNNFLLDGVYNLDPKLNTFGVRPPVDAIREF
                     EMLTSSYDASFGRNPGAQINVVLKSGTNDFHGSLFEFHRNAALDARNVFAPANEPKPK
                     YLRNQFGGSIGGPIIKDRTFIFGDYEGTRSREGITRITNVPTLAERQGDFSQSLFAPP
                     INPFTGTPFPGGVIDPLFIHPIGRAIASLYPLPNRNVPFRNFVSSPTQRDDNDSFDVR
                     LDHRVTQRADLTFRYSFGDRNLFEPFTGPTLSLVPGFGDTVKRRSQNAMTALTLVLTP
                     NLVNETRAAFSRVAASVTQEASRLNRDLGLPTISPNPRDAGLSFITVTGLSPLGDEGN
                     NPQNSVTNVYQLINTSSYSHGRHLIKFGGDVRFSQQNAFRDVESRGRLTFSPFAQISG
                     NALADLLLGFPLLTSVARVDNPQRLRTQSYNAFFNDSLRLSPRLTLNAGLRYEFNSPP
                     VDADDRANVYDLATRNLVRVGTNGVPRGGIEADKNNFAPRVGLAWTVGKDEKTVIRAG
                     YGIYYDQSA"
BASE COUNT          569 a          612 c          697 g          558 t
ORIGIN      
        1 cgcagactga tcgtagtaga tgccataccc ggcacggata actgttttct catcctttcc
       61 gacggtccac gcgaggccaa cgcgcggcgc aaagttattc ttgtcggctt caatgccacc
      121 gcgcggcacg ccattagttc cgacgcgcac gaggttacgt gttgcaagat catagacgtt
      181 cgcgcggtca tcagcatcga ccggaggcga gttgaactcg tagcgcaggc ccgcattgag
      241 tgtcaaacgc ggactgagac gcaggctatc gttgaagaag gcattgtaac tttgcgtgcg
      301 caatcgctgc gggttatcga cgcgcgcgac actcgtcagc agcggaaaac cgagtagcag
      361 atccgccaaa gcattacctg agatttgcgc aaagggcgag aacgtcaggc gaccgcgcga
      421 ttccacatcg cgaaaagcgt tctgctgtga gaaacgaaca tcgccaccaa acttgattag
      481 atgacgtccg tgcgaatagc ttgaagtatt aatgagttga tagacgttgg tgacgctatt
      541 ctgcggattg ttaccttcgt cacccagcgg tgaaagacct gtcacggtga taaaactcaa
      601 cccggcatcg cgggggttgg gcgaaattgt cggcagtccc aagtcgcgat tgagccggct
      661 ggcttcctga gtgacagacg ctgcaacccg gctaaaagcc gcgcgagttt cattaacgag
      721 attgggggtt aatacgagcg tcagtgcagt catcgcgttt tgactgcgac gcttgactgt
      781 gtcgccgaag ccaggcacca aagacagtgt cggcccggtg aacggctcga agagattccg
      841 gtcgccaaag ctgtaacgga acgttagatc cgctcgctgc gtaacgcggt ggtcgagccg
      901 cacgtcgaaa ctgtcgttat cgtcgcgttg cgttggcgac gagacgaagt tacggaacgg
      961 tacgttccga ttcggtaacg gatagagcga ggcaatggct cgaccgatgg ggtgaataaa
     1021 caacgggtca atcacgccac caggaaaagg cgttccggta aagggattga tggggggtgc
     1081 gaacaggctt tgtgaaaagt cgccctgtcg ctcggccagt gtcggcacgt tggtaatgcg
     1141 ggtgatgcct tcgcgcgagc gcgtgccttc gtagtcgccg aaaatgaagg tccggtcttt
     1201 gataatcggc ccgccgattg atccgccaaa ctggtttcgg aggtacttag gttttggttc
     1261 attcgccgga gcaaagacat tgcgagcatc gagagcagca ttgcgatgga attcaaataa
     1321 ggagccgtgg aaatcattcg tgcctgactt gagcactacg ttaatctggg cgcccggatt
     1381 tcggccaaac gacgcgtcat aactgctggt gagcatttca aattcgcgaa tggcatcgac
     1441 cggtggacgc acgccgaagg tgttcaactt cggatcgagg ttatagacgc catcgagcag
     1501 gaagttgttg ctgtcttcac gcgcgccgtt aacgttgaaa gcaaaatcgc cgcgaacgga
     1561 accggcggaa ccgggagccg ctggcacggc gccgggcacg agcagggtga gctcataaaa
     1621 gtttcgacca tcaagcggta gcccgctgac ctggcgattc tcgattactg taccgatgga
     1681 ggaagaatct tttttaagct cggaaggttc gtcaatgaac attccctcat gggtgacggc
     1741 gcctacgaac aaagtcacgt cgagtcggag ttcctgatct actttcagga ctacttcttg
     1801 acctgatttt tggaaattag gaaatgtaac ctccaaaaga tacgtgccgg gttcaagtga
     1861 tgtgagtgaa tattcacctt gagggccact catcgccgtc cgttctttgt tagtctgccg
     1921 attggttagt ttcagttcag ccccgggaat cacggcttgt cgtgggtcga agatcgtacc
     1981 gcggaccgtt gcccgatgtg tttgcgcact cgccaatgtc ggcaacagga tcagcaaagc
     2041 cagcatcaga gttttcacta tcgttgacct caagttggat ctccttactt gttcctcgga
     2101 gggaactacg aaaaattgga caggatttct tgctgtccgg gtattattac cgagtaggtg
     2161 cgaagtagcg caagctgtca gcgtgcgtgc aaactaacag tttgcggaaa acaagatatc
     2221 gagtttttcg gctagtgtgt gaaactgatt gctagtacac gccgtattca ggcctaatgt
     2281 cccgcatgat caaaccggag cgatacctat gatggatctt tcgcaatttt cgtttccagc
     2341 ctttattcaa agactttcag tcctcgtcgg ccttctcagt gttctcgctt ttactgcggc
     2401 ggcccaaacc aacggcacaa ttaccgggga cattaa
//
LOCUS       DASUEX010000486         2209 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_649525, whole genome shotgun sequence.
ACCESSION   DASUEX010000486 DASUEX010000000
VERSION     DASUEX010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_649525"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2209)
                     /locus_tag="VFV61_08160"
     CDS             complement(<1..>2209)
                     /locus_tag="VFV61_08160"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HEX4900490.1"
                     /translation="VLQSRIDPASSRVIAHNMAAVTLAYGRAISDDGLRVVYSAETVP
                     NSSQVFLWDGRNNLTRQITFLEARDEDVSLQATISGDGSRIAFATRRNVIGGNPDHSV
                     ELYLLDLPSGQIESVSSAPASATAEIISSLNDDGSRVVFNFPRVLSGDVSSSGFANNS
                     EIYVGTLSPRPPFGSLSILNGAAPDAGQGIDGSIAPSSIALAQGSALGFSTQEAARQA
                     DGSFPLRVSGTTVTVNGRAAQIFFVSPEQVNFLVPAATEVGIAEIIITNPDGFISKSM
                     VSIAATAPGIFSTNGQGSGAGVILNADTLQAGPFDPSSGTLRLSLFATGVRNASNASV
                     MASGRPLTLESVHASRELPGLDELHVLIPAAFHGAGTIALVVEADNHFSNMVEVTFTG
                     SLTQNVLINEILADPPDGVAGDANHDGVRSSSEDEFVELVSNGGAANISGWTIRTRSL
                     GGASETTRHTFPSGTLLLAGDAIVVFGGGNFSPADPIFGCAQITSTSTAGLSLVNGGL
                     SVLIRDAAGSLVTEFTYGGTTGLEGDDNQSLTRSPDVSGTFVEHLAGAEANGRRFSPG
                     LRTDGTPLVDCAARLQSLTLSAAAPTIDVGGMTSVVARPLDTYGRPLPNVAVSFASDN
                     PAVATVDAVSIDEATGTFTAMITGHAPGTALIDAEATEGETNIAATVAITVLNPATPP
                     LLVINQIYGGGNNSNATFQNDFVELFNRGTTAVDFAATPYSLQYASAAGNFTSA"
BASE COUNT          495 a          610 c          652 g          452 t
ORIGIN      
        1 ggcgctggta aagtttcctg cggcggaagc gtattgcaaa gaataagggg tcgctgcgaa
       61 atcaacagcg gtggttccac gattgaacag ttcaacgaaa tcgttttgaa aggtggcgtt
      121 ggagttgttg ccgccgccgt aaatctggtt gatgactagc aacggaggtg ttgctggatt
      181 taggacggtt atcgctaccg tcgcggcgat attcgtttcg ccctcagtcg cctcagcgtc
      241 gataagggct gtgcctggtg cgtgaccagt tatcatcgcc gtgaacgttc cggtcgcctc
      301 atcaattgag actgcgtcca cagtagccac ggctggattg tcagaagcaa aactcacagc
      361 cacattggga agcggtctcc cgtaagtgtc gagtggccgc gccacgacgc tggtcattcc
      421 gccaacatcg attgtcgggg ccgccgccga gagcgtgagc gactgaagtc gtgcggcgca
      481 atcaaccaga ggtgtaccgt cagttcttaa acccggcgaa aaacggcggc cgtttgcttc
      541 agcgccagca aggtgctcga caaaggttcc gcttacatcc ggtgagcggg ttaacgattg
      601 attgtcgtcg ccttcgagac ctgttgtgcc tccataggta aattcagtga cgagggagcc
      661 agcggcatcg cggatcagga cggacaaccc accattcact aacgacaggc ccgccgtcga
      721 agtgctggtt atctgagcgc atccgaaaat gggatccgcc ggactgaaat tgcctccgcc
      781 aaacaccacg atcgcatcgc ccgcaagcag cagagtgccg gagggaaaag tgtgtcgcgt
      841 ggtttcgctt gcgccgccga ggctccgggt gcggatcgtc caaccggaga tgttcgcagc
      901 gccaccgtta cttaccaact caacgaactc atcttcactg gagctacgca cgccatcgtg
      961 gttcgcatcg cctgccactc catcaggtgg gtccgcgagg atctcattta tcaaaacgtt
     1021 ctgcgttagt gagcccgtga aagttacttc gaccatgttg ctgaagtgat tatccgcctc
     1081 cacaacgagc gcaatggtcc ctgccccatg gaatgcagcc ggtatgagta catgcagttc
     1141 atcgagccct ggaagctcgc gagacgcatg aactgactcc aaagtgagcg gacgacctga
     1201 cgccatcact gatgcgttgc tggcatttcg taccccagtc gcaaataagc tcaaacgaag
     1261 ggtcccactg ctgggatcga atggtccggc ttggagcgta tccgcattca gaatcacgcc
     1321 cgctccagat ccctggccat ttgtggagaa aatacccggg gcggtggccg cgattgaaac
     1381 catgctcttg ctgatgaagc cgtccgggtt tgtgatgatg atttcggcga taccgacctc
     1441 agtcgcggct ggaaccaaaa agttcacttg ctccggcgaa acaaagaaga tttgggctgc
     1501 gcgcccattg acggtcacgg tcgtgccgga aacgcgcaag gggaaactgc cgtctgcctg
     1561 acgcgcggct tcctgcgtgg agaagccgag agcgctgccc tgcgccagtg cgatgctgct
     1621 cggagcaatc gaaccgtcaa ttccttgacc ggcatcgggt gccgccccgt ttaagattga
     1681 gagtgatccg aacggcggtc gaggagaaag tgtcccaacg tagatctcgg aattattcgc
     1741 aaagccactc gaactgacat cgccagacag aacgcgtggg aagttgaaga ctacacggga
     1801 gccatcgtcg tttagggaag agatgatctc ggcagttgcg ctggcgggcg cgctagagac
     1861 ggactctatc tgtccagacg gaagatcaag gaggtagagt tcgacgctgt gatccggatt
     1921 accaccaatt acgtttcgtc gagttgcaaa cgctatccgc gaaccgtcac cgctgatagt
     1981 tgcttgcagg gaaacatctt catcacgggc ctccaggaaa gtgatctggc gcgtaaggtt
     2041 gttgcggcca tcccagagaa acacctgact cgagttcggc accgtctcgg ctgaatagac
     2101 cacgcgcaag ccatcgtcag aaatagctcg gccgtaggcg agcgtcacgg ctgccatgtt
     2161 gtgggcgatg acgcgagagg atgccgggtc aatccgcgac tgtagaact
//
LOCUS       DASUEX010000487         3473 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_419657, whole genome shotgun sequence.
ACCESSION   DASUEX010000487 DASUEX010000000
VERSION     DASUEX010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3473
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419657"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            93..944
                     /locus_tag="VFV61_08165"
     CDS             93..944
                     /locus_tag="VFV61_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900491.1"
                     /translation="MIIISSFVRLSVMVMLLPLSAAHAITQTDSIPRGQIVDRIEALN
                     DSSQSYALYLPSNYTPQRKWPVLYAFDPGARGRVPVERFKEAAEKYGWIVLGSNNSRN
                     GPWNVVVNAWNAMQKDTHQRLAIDDERMYATGFSGGARVAVHIAAGCKCLAGVMANGA
                     GFPVDLAPSPQMHFVFFGAVGVDDFNYPELKGLNEPLTKAGIIHRVQTFDGRHEWPPV
                     SVATAAVEWMELQAIKAGKRPRDDDFVNVTWQQILNDARTLEEAKKYNEAYQLYLDLA
                     LTFNGPN"
     gene            1060..2304
                     /locus_tag="VFV61_08170"
     CDS             1060..2304
                     /locus_tag="VFV61_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HEX4900492.1"
                     /translation="MKARQEVTKATAGQYRGARKKEKGQILDQFIATTGYSRWYARLV
                     LRHQGRRIQTDKQTIVVVERKASVQRKRARRYDEKVQTALIRLWRIMDYICGKRLQPM
                     LAELLPVLERHNEFRCDRETRAKLLRISAASIDRLLKPERRKYELRGRSGTKPGTLLK
                     KQIPIRTFAEWDEQRPGFVEIDLVAHDGGLAAGDYCQTLDLTDIATTWTETQAVRNKA
                     QSWVFAALKEMRQKLPFSLLGIDSDNGSEFINKDLVEYCQKQKLSFTRSRPYRKNDNC
                     FVEQKNYSVVRRAVGYQRYDTEAQLHLLNELYATLRLYTNFFQPTMKLKSKERVGSKV
                     IKRYDRAQTPYQRVLAAAQVSEAEKQRLRAKYKTLNPAALKRKLMRLQERLMKSTASR
                     KAARVRPRQCRNDRYFSKQPER"
     gene            2653..>3473
                     /locus_tag="VFV61_08175"
     CDS             2653..>3473
                     /locus_tag="VFV61_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900493.1"
                     /translation="MKRIVILIVLVALAAVAGIVRSSVGSVAELRQLASHKNTADVRQ
                     EVRQTYELAPGARVELSGLNGAVKIETSDTKTAEIYIERTASTQEAMDRRKVTIEADA
                     NSLRIRAEKSDDAGFFSRFFGSSANERTTLKLPRQIALYAKGVNGAFVTSDIDGAVEI
                     TGVNGRVQVGSAVGTATFKGINGNMVVGLKKIEQEGVTLSGINGNIELQLGADVNADF
                     DARGLNGRVISDLPNVAIDKEKRGKYWARIGTGGAGITAKGINGNIRITRVAAVET"
BASE COUNT          834 a          881 c         1020 g          738 t
ORIGIN      
        1 actacttgcg gatggccgac aattcggcaa catggcaaat ttgaacgaag caacccatat
       61 tccccaaaat cataacggct ggtgactgaa cggtgatcat tatttcttca ttcgtgcgct
      121 tgagtgtgat ggtcatgcta ctgccactct cagccgccca tgcaatcaca caaaccgact
      181 ccatcccaag aggccaaatc gtcgacagaa ttgaagcgct caacgattcg tcccaaagtt
      241 acgcgctgta tctcccgtcg aattacacgc cgcaacggaa gtggccggtt ctgtacgcct
      301 tcgacccggg tgcgcggggc cgggtgccgg ttgagcgttt taaggaggcg gcggagaagt
      361 acgggtggat cgtgcttggc tcgaacaatt cacgcaatgg tccatggaac gttgtcgtga
      421 atgcgtggaa cgccatgcag aaagatactc atcagcgttt ggcgatcgac gatgagcgta
      481 tgtatgcgac cggtttttcc ggcggcgcac gcgttgcagt ccacatcgcg gcaggctgca
      541 agtgcctggc aggcgtaatg gccaatggcg cggggtttcc tgtcgatctt gctccgtcgc
      601 cacagatgca ttttgttttc tttggtgcag ttggcgtcga tgattttaac tatcccgaac
      661 tcaagggcct aaacgaaccg ctaaccaagg caggcatcat ccatcgcgtt cagacgtttg
      721 atggtcgtca cgaatggccg ccggtctcgg tggctacggc agcagttgag tggatggaat
      781 tgcaggcaat aaaggccggc aagcgcccgc gcgatgacga ctttgtaaac gtaacgtggc
      841 aacagatcct taatgacgca agaactcttg aggaagcgaa aaaatacaat gaagcgtatc
      901 aactctatct cgatctggcc ctgaccttca acgggccgaa ttgactcatc aaaaacccac
      961 tccaaatggc attcgttgcc tcatttaaaa acccactcga aatagcgggt gttttagctc
     1021 cagtgtttga cggcactgga ggtgaggatg aggctaacga tgaaggcaag gcaagaagtt
     1081 accaaagcga ccgccgggca atatcgtggc gcgcgcaaaa aagaaaaagg ccagattctg
     1141 gatcagttta ttgccaccac tggctatagt cgctggtatg cgcggttagt gttgcggcac
     1201 caaggacgtc gcatacagac cgacaagcaa acgatcgtgg tggtggagcg caaggcgagc
     1261 gtgcagagaa agcgcgcgcg tcgctatgac gagaaggtgc aaacagcgct gatcagactg
     1321 tggcgcatca tggactacat ctgcggcaag cgcctgcagc caatgctggc ggagctgctc
     1381 cctgtcctcg agcgccacaa tgagttccgt tgcgatcgcg agacgcgcgc gaagctgttg
     1441 cgcatcagcg ccgccagcat cgaccggtta ctcaaacctg agcgccgcaa gtatgaactg
     1501 cggggccgct cagggaccaa gccgggcacg ttgctcaaga agcagatccc gattcgcacc
     1561 ttcgccgagt gggacgagca gcggcctggg tttgtcgaga tcgacctggt agctcatgac
     1621 ggcggcctgg cggccggcga ctattgtcaa actttggatc tcactgacat tgctaccacc
     1681 tggaccgaga cgcaggctgt ccggaacaaa gcgcaaagct gggtcttcgc cgctttgaag
     1741 gaaatgcgcc aaaaactgcc gttttctttg ctgggcatcg atagtgacaa cggctccgag
     1801 ttcatcaaca aggacttggt ggaatactgt cagaaacaaa agctcagctt cacgcgctcg
     1861 cgtccctacc gcaaaaacga caactgtttt gtggagcaga agaactattc ggtagtgcgg
     1921 cgcgcggtgg gctaccagcg ttatgacacg gaagcgcagt tgcacttact taatgaactc
     1981 tatgcgacct tgcggctcta cactaacttc tttcagccca ccatgaaact aaagtcgaaa
     2041 gaaagagtgg ggagcaaagt aattaagcgt tatgatcgcg cccaaactcc ttaccaacgg
     2101 gtgctggcgg cggcgcaggt aagcgaagcg gagaagcaac gcttgcgcgc caaatacaaa
     2161 actctcaacc cggcggcgtt gaaaagaaaa ctgatgcgtt tgcaggagcg cttaatgaag
     2221 tctactgcta gcaggaaagc agcgcgtgtg cgcccgcggc aatgccgcaa cgatcgctac
     2281 ttctctaagc agcctgagag ataagaggat gagtttatcg ctttaccgag taccggatgg
     2341 gcagcaaaaa gggcagagcg tgttgccctg cccatccgta tactcaccgg cgacggcgct
     2401 cgggtcgctc tccagcgttg ccctatcctc cgcgtcggcg ccagtgactc tagcacagag
     2461 ccgcgaacaa gccgcaaact accaacattt ttgcgctcgc atttggagtg ggtttttaaa
     2521 tgaggcaacg aatgctattt cgagtgggtt tttacgtgag gcaatagggg tggcaattct
     2581 ttcgaacaaa tctgcgccgc ctgtcgttaa aggctccgag tgtcattttt acttcctttt
     2641 tgaggccgaa atatgaaacg catagtgatt ctgatcgttc tggtggctct cgccgctgtt
     2701 gctggtattg ttcgttcttc tgtgggctcg gttgcggagc tacgccagct tgcttcacac
     2761 aagaatacgg ccgatgtgcg gcaggaggtc cggcagactt acgagcttgc gccgggcgcg
     2821 cgggtcgagc tcagcggcct gaacggcgcg gtaaagattg agacttccga cacgaagacc
     2881 gcggagattt atatcgaacg caccgcgtcg acccaggaag caatggaccg tcgcaaggta
     2941 acgatcgagg ctgatgcgaa cagtttgcgt attcgtgctg agaaatcaga cgatgccgga
     3001 tttttctcgc ggttctttgg ttcgtccgca aacgagcgca cgacattgaa gctgccacgg
     3061 cagatcgcgc tttatgcgaa gggtgtgaat ggagcgtttg ttacgagcga cattgatggt
     3121 gcggttgaga tcactggagt gaatggccgc gtgcaggttg gtagtgcggt cggaactgcg
     3181 acgttcaaag ggatcaatgg aaacatggtg gttggtctca agaagattga gcaggagggt
     3241 gtgacgctca gcggcattaa tggaaatatc gaattgcagc ttggagcgga tgtgaatgct
     3301 gacttcgatg cacgcgggtt gaatggacgc gtgatttccg atctgccgaa tgtagcgatc
     3361 gataaagaaa agcgcgggaa atactgggcg cgcattggca ctggcggcgc cgggatcacc
     3421 gcgaagggga ttaacggaaa tattcgaatc acacgtgttg ccgcggtgga aac
//
LOCUS       DASUEX010000488         3522 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1673017, whole genome shotgun sequence.
ACCESSION   DASUEX010000488 DASUEX010000000
VERSION     DASUEX010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3522
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1673017"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            195..1934
                     /locus_tag="VFV61_08180"
     CDS             195..1934
                     /locus_tag="VFV61_08180"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX4900494.1"
                     /translation="MIQMLSLRLRAVLACALLIFIIQGGALGQQTAKTKAAQIAEVMT
                     LANKYRQFNGSVLVADHGKVIYKKGLGLANMEWEIPNTPETRFRLGSITKQFTATAIL
                     QLVEQGKIKLDGKISDYLPDYRKDVGEKVTIHQLLNHTSGIPSYTGLPGFFQDVSRNP
                     FKVDDFIKKYASKDLEFEPGSKFSYNNSGYFLLGAIIEKVTGKPYEQVLKENIFDPLG
                     MKNTGYDHFDTLIQKRATGYQKTANGYSNAPYLDMSIPYAAGSLYSTVEDLYLWDQAL
                     YTDRLLKEETKQLMFKPNLSNYAYGWTIRKTRFDEKIPVITHNGGINGFSTTIIRFPG
                     EKNLIVMLDNTAQGNSIDRLSEELTKILYERPFELPKMSVAEVLFKTINEKGITAAMA
                     QFQELKAKQANSYDFSEQELNRLGYQLMGTGKRDEAIEIFKLNVDLYPKAFNTYDSLG
                     EAYMAAGNKELAIANYKKSLELNPQNTNGTEMLKRLEGTAAPVDAKSYEAYVGEYEVT
                     PSFIVRMFIEGDKLMTQATNQPAFELSPEGEDKFFLKVVNARVTFTKDEKGAVTGLII
                     HQGGRDVPGKKIK"
     gene            complement(2031..3272)
                     /locus_tag="VFV61_08185"
     CDS             complement(2031..3272)
                     /locus_tag="VFV61_08185"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX4900495.1"
                     /translation="MRHLKVRTIGSIVFLILLSVGEAAAQAGGNWPQWRGPNRDGIST
                     ETGLLKQWPESGPPLVWKAAGAGFGYSSFAVVNGRLYTMGLRGDREYIIAFDVANGKE
                     VWATPHGGAFRNDRGDGPRGTPTVEGNRLYALGGDGDLSALDVATGKIIWTMNVLKKF
                     GGEQITWGISESPLIVGDKLLVNPGGPGASIVALNKKDGALIWKSQNEKAGYSSAIAL
                     EAAGTPQVVFFTSTRAVGLDLKDGRLLWEYGKPANRVANAATPIARANRVFISSDYGT
                     GGGVVEIKANGKDMAAQELYFTKDMRNHHSSSVLIGDHLYGFSSSILTAMKFDTGEIA
                     WKDRSVGKGSLVYADGLLYLFSENGVVGLAEATPAGYKEKGRFRIQQESLPTWAHPVV
                     AGGRLYLRDQDTIYAYDVKAK"
     gene            complement(3332..>3522)
                     /locus_tag="VFV61_08190"
     CDS             complement(3332..>3522)
                     /locus_tag="VFV61_08190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900496.1"
                     /translation="AFRSGGLPTYVMPLVFAGAPLVNVLFSMWLHPPKTMPNPLLYLG
                     FVLAAVGAGMVLYFKPPA"
BASE COUNT          898 a          979 c          908 g          737 t
ORIGIN      
        1 cctgacgctt gcacacgatg ggcccagccc agaataagga tgaagccaaa tacggcgcaa
       61 agccactgcc tcacatcccg cggcaaagcc gtaccgttca aggctcagac aaactaagca
      121 gcgcgaaact atttctatca agccatcgta actttctccg gttcacttag ccctacttgc
      181 aggagactcc attcatgatt caaatgcttt ccttgcgact tcgcgctgta ctggcgtgcg
      241 cacttctcat cttcatcatt caaggcggcg cccttggcca acaaactgct aaaaccaagg
      301 ccgcacaaat cgccgaggtg atgacgcttg ctaacaagta ccggcaattc aatggttccg
      361 tgctggtagc cgatcatggc aaggtgatct acaagaaggg cctcggcctg gccaatatgg
      421 aatgggaaat tcctaacacg cccgaaacca ggtttcgcct cggttccatc accaagcagt
      481 tcaccgcgac ggcgatcctg caattggtcg aacaaggcaa aataaaactc gacggcaaga
      541 tttccgatta cctgccggac tatcgcaaag acgtcggcga gaaagtcacc attcaccaac
      601 tactcaacca tacgtcaggc attcccagtt acaccggtct gccaggattc tttcaagacg
      661 ttagtcgcaa tcccttcaag gtggacgatt ttattaagaa atatgccagc aaggatctgg
      721 aatttgagcc cggcagcaag ttctcttaca acaattccgg ctactttctc ctgggtgcga
      781 tcattgaaaa ggtgacaggc aaaccctacg agcaggtttt gaaagagaac atcttcgatc
      841 cactgggaat gaaaaatacc ggatacgacc actttgacac gctgatccag aaacgcgcga
      901 cggggtacca aaagacagcc aacggttaca gcaacgcgcc ttacctcgac atgtcgatcc
      961 cttatgccgc tggctcgctg tattccacgg tcgaggacct ttacctctgg gaccaggcgc
     1021 tctataccga tcgcctgctc aaggaagaaa cgaagcagtt gatgttcaag cccaatctct
     1081 caaactacgc ttacggctgg accatcagga agactcgctt cgacgagaag attccggtaa
     1141 taacgcacaa tggcggcatc aatggtttca gcacgaccat tatcagattt cccggcgaaa
     1201 agaacctgat tgtgatgctt gataacacgg cgcaggggaa tagcatcgat cgactgtcag
     1261 aggagttgac gaagatcctt tatgagcgcc cttttgagct gccaaagatg tcggtggctg
     1321 aagtgctgtt taagacgatc aacgagaaag gcatcactgc cgccatggcg cagtttcagg
     1381 agctaaaagc gaaacaggca aacagctacg acttcagcga gcaagaactc aaccgactcg
     1441 gctatcagtt gatgggcacg ggcaagcggg acgaggcgat agaaatcttc aaactaaacg
     1501 tcgacctcta tccgaaagcc ttcaatactt acgacagtct gggcgaagct tacatggctg
     1561 cgggtaacaa ggaactggcg attgctaact acaagaagtc gcttgagctg aatccgcaaa
     1621 acacaaatgg gactgagatg ttgaagcggc tcgagggcac agccgcgccg gtcgatgcga
     1681 agtcctacga ggcctatgtc ggtgagtatg aagtgacgcc gagttttatc gtcaggatgt
     1741 tcatcgaagg cgacaagctg atgacgcagg ccactaacca gcctgctttt gaactttcac
     1801 ccgaagggga agataaattc ttcctgaaag ttgtgaatgc cagggtaacg ttcaccaaag
     1861 acgaaaaggg cgcggtcact ggtttgatta ttcatcaggg tggccgcgat gttcccggaa
     1921 agaagattaa gtaaagattc aaaatacaac gtccaaggtc ctagcgtccg ttagcgagac
     1981 ttggcgcaca cgggcattgg actttggacg ttggaccttg gacgttcgaa ttacttagcc
     2041 ttcacgtcgt aagcgtagat tgtgtcctga tcgcgcaggt agaggcgacc cccggcgacc
     2101 accggatggg cccaggtcgg cagcgattcc tgctgaatgc gaaagcgtcc cttctctttg
     2161 taaccagccg gtgtcgcttc cgccaagcca acaacgccgt tctcgctaaa aaggtaaagc
     2221 agaccatccg cataaacgag tgaaccctta ccaacgctgc gatccttcca ggcaatctca
     2281 cccgtatcaa acttcatcgc cgtcagaatg ctgctggaga agccatagag gtgatcacca
     2341 atcagtactg agctgctgtg atggttgcgc atgtctttgg tgaaataaag ttcctgggcg
     2401 gccatgtctt tgccgttggc tttaatttca accacgcctc cacccgtgcc gtagtcagat
     2461 gaaataaaga cacggttggc gcgcgcgatc ggagtggccg cgtttgccac ccggttcgcc
     2521 ggtttgccgt actcccacag caaacggcca tctttcaaat ccaatcccac tgccctggtg
     2581 gaagtaaaga agacgacttg cggagtgccg gcagcttcga gggcgattgc cgaggaatat
     2641 ccggccttct cattctgact cttccaaatc agggcgccgt ccttcttgtt aagcgccacg
     2701 atcgaagctc ccggtccgcc tggattgacc agcagcttgt cgccgacgat tagcggtgat
     2761 tcgctaatgc cccaggtgat ttgttcgccg ccgaatttct tcagcacatt catcgtccag
     2821 ataatttttc cggtggcaac gtcgagagct gaaagatccc catcgccgcc gagagcatag
     2881 agccggttgc cttcgactgt tggcgtaccg cgcgggccat cgccgcgatc gttccgaaac
     2941 gcaccgccgt ggggtgtggc ccaaacttcc ttgccgttcg ctacatcgaa cgcaatgatg
     3001 tactcgcgat caccgcgcag acccatggta tagaggcgcc cattaactac cgcgaacgag
     3061 gagtatccga atcccgctcc agctgccttc cagacgagcg gcggcccgct ttccggccac
     3121 tgctttagca accctgtttc agttgaaatg ccatcgcgat ttggcccgcg ccactggggc
     3181 caattgccgc ccgcttgcgc cgcggcttcg ccgacagata gcagaatcag gaacacgatg
     3241 ctgccgatag ttcgcacttt gaggtgtctc atttccggga tctcctttag taaaagaacg
     3301 gaagcgggga gagatagaac aacaacaaaa cctaggccgg cggtttgaag tagagcacca
     3361 tgccggcgcc aactgcggcc aacacaaacc caagatagag tagcgggttg ggcatggtct
     3421 taggcggatg cagccacatc gaaaaaagca cgttcaccag cggtgctccg gcgaatacta
     3481 agggcatgac atacgtcggc aggccgccgc tacgaaaggc aa
//
LOCUS       DASUEX010000489         2759 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_767994, whole genome shotgun sequence.
ACCESSION   DASUEX010000489 DASUEX010000000
VERSION     DASUEX010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_767994"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="VFV61_08195"
     CDS             complement(<1..257)
                     /locus_tag="VFV61_08195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900497.1"
                     /translation="MKEWVALNMTPGIGPRAAAKLLERFGSAEAVYCATRAELEKLRL
                     LPEAVESIIARDLFVRAEAEIESVRRLGADLLILDDGVYPAL"
     gene            complement(712..810)
                     /locus_tag="VFV61_08200"
     tRNA            complement(712..810)
                     /locus_tag="VFV61_08200"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(774..776),aa:Sec,seq:tca)
     gene            1147..>2759
                     /gene="boxC"
                     /locus_tag="VFV61_08205"
     CDS             1147..>2759
                     /gene="boxC"
                     /locus_tag="VFV61_08205"
                     /EC_number="4.1.2.44"
                     /inference="COORDINATES: protein motif:HMM:TIGR03222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_process: GO:0010128 - benzoate catabolic process via
                     CoA ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-epoxybenzoyl-CoA dihydrolase"
                     /protein_id="HEX4900498.1"
                     /translation="MINFQTAPEHYHHWKLSIDGPIAHLSMDVQEDATLVEGYKLKLN
                     SYDLGVDIELADAVQRIRFEHPEVRVVVVTSLKPRIFCAGANIYMLGTSSHAFKVNFC
                     KFTNETRCAIEDDSRHSGRRYVAALNGTASGGGYELAIACDEIYLVDDGNSAVSLPEV
                     PLLGVLPGTGGLTRLVDKRKIRRDRADVFSTLAEGLKGKRAKEWGLIDDYFPTSKFQE
                     AVANKVQNMIAGNKWNSGPGIKLNPVEFKASDAARDYKYVSVKLNREQRYADLTLRSP
                     EADLPTSPDEIQKLGDKYWPLQAYRELDDLLLHIRVNEPEIGLVCLRTEGDIDRVLAV
                     DRTLAEHADHWLIREIILHMARVLRRLDLTAKSFFALIEPDSCFAGNLLELALASDRV
                     YMLNDPDASETSIAVSELNAGALPMSNGLTRLQSRFQAEPERVARVLAPAGRLDTEAA
                     DEAGLITFAPDGLDWEDEIRVAIEERTSLSPDALTGMEASLRFAGPESVDTKIYGRLT
                     AWQNWIFQRPNAVGEKGAPSLDGQVDRLH"
BASE COUNT          648 a          765 c          734 g          612 t
ORIGIN      
        1 agcgcgggat agacgccatc atcaagtatt aaaagatcag cgccgagacg acgaacgctt
       61 tcgatctcgg cttccgcccg gacaaacaaa tcgcgagcga tgatgctttc gactgcctct
      121 ggcagcagtc gcaatttctc aagctcagct ctggtggcac agtaaacggc ttcggcggat
      181 ccaaagcgct ccaaaagctt ggcagcggcg cgaggcccga tgccgggggt catattgagg
      241 gcaacccatt ctttcatacg attgcagact tcggagtgag gattgcggat tggactcgtt
      301 caaatccgcg gtctgcgtag cgctagtgct gacgctttgc cgttttaatc gaagcaacaa
      361 ctatagcgag aatctcgtct gcttcgctca tcaagtcggc aattcgtttt ggtttcacaa
      421 ttcccgtatc aattagtaat tacatccaat agatcgcgtc tgcttcttct accgttccca
      481 tctttgagac aaaatcaggc tttgctttgc ctcgacacgc agagtggtaa ttagcaccga
      541 ccgacgaacc gcaacgaatc aattggtttc tgattacccg cgcagtcggc gtgtccggca
      601 agcactcggc caagcggaag gctcttaacg caaagcgccg tgtccgactc tttaactagt
      661 ctggattcat agatcggcca caatctaaaa tccgcaaacc gcaatcgtat ctggcggagg
      721 cgtgtaggaa tcgaacctac ctgagcctgc tctcacagtc tcactgacgg ttttgaagac
      781 cgcgccgttc accagaacag attcgcctcc agcacggtac cactagagcc ggaagagcca
      841 gggaccaccg aaggctgaca accctacgcg ccgaaatgcg agactgtcaa gcgagggcca
      901 gaagcgactg ggcgcagctc gttgctcgat ttccagtttt cattcccatc cgctcgcccg
      961 ctgatttggc gccaaaacga tggacttcca ctaagacctg gcccccgaaa aatccctatc
     1021 caaacttgac cgcaacaaaa gtcaccagct aatatcagcc tttcccgaac gaacgttcga
     1081 accggcaagg gccggactct tggtcggaaa gaccaaaagg tcaactccga cttaaccgag
     1141 gtatggatga tcaactttca aaccgctcca gaacattacc atcactggaa attgtctatt
     1201 gacggcccca tcgctcacct ttccatggat gttcaggaag acgcgaccct cgtagagggc
     1261 tacaaactca aacttaattc ttacgacctc ggcgtggata tcgagctggc tgacgcggtg
     1321 cagcgcatcc gcttcgagca tcccgaagtg cgggtcgtgg ttgtcactag tttgaagcca
     1381 cgtatctttt gcgcgggtgc gaatatctac atgctgggta cgtcctcgca tgccttcaaa
     1441 gttaacttct gcaagttcac caacgagaca cgctgcgcta ttgaagacga ctcgcgacat
     1501 tcaggccgcc gctatgtggc cgcactaaac ggcaccgcct caggtggagg ctatgagtta
     1561 gcgatcgctt gcgatgagat ctatctcgtg gacgatggta actcggcggt ttccctgccg
     1621 gaggttcccc tcctgggcgt gctccccggc accggcggct taactcgcct cgttgacaag
     1681 cgcaagattc gccgcgatcg ggccgacgtt ttttccacgc tcgctgaagg gctgaagggt
     1741 aaacgagcga aggagtgggg actgatcgac gactattttc cgacgagtaa gtttcaggaa
     1801 gcagtcgcca acaaagtcca gaacatgatt gctgggaata agtggaactc cgggcccgga
     1861 atcaaactga atccggtgga attcaaggcg agcgatgcgg cccgggatta caagtacgtt
     1921 tccgtgaagc tgaaccgcga gcagcgttat gcagatctga ccctgcgcag cccggaagct
     1981 gatttaccaa cgtcgccaga tgaaattcaa aagcttggcg acaagtattg gccgctccag
     2041 gcttatcgcg aactggatga tctgttgttg catatccgcg ttaatgaacc agagattggc
     2101 ttggtttgcc taaggacgga aggtgacatt gatcgagtct tagccgttga tcggaccctt
     2161 gctgagcacg ccgaccattg gctgattcga gagatcatct tacacatggc gcgcgtcctg
     2221 cgccgtctcg atctaacagc gaagagtttt tttgctctca tcgagccgga cagttgtttc
     2281 gccggcaatc tgctggaact cgcactcgct tccgatcgcg tctacatgct gaatgatcct
     2341 gacgcgtctg aaacatcaat tgcggtgtca gagctgaacg ccggagcatt gccaatgagc
     2401 aacggattaa cacgtttgca atcgcgcttc caggccgaac cagaacgcgt cgcccgagtg
     2461 ctcgcacccg ctggacggtt ggacacggaa gcagccgatg aagctggcct aatcacgttc
     2521 gctccggacg gtcttgattg ggaagatgag atccgcgtcg cgattgagga aagaacgtcg
     2581 ctctctccgg atgctctgac gggtatggag gcttcgttaa ggtttgccgg tcccgaatca
     2641 gtggatacaa agatctacgg tcgtctgacg gcttggcaaa actggatctt tcagcgaccc
     2701 aatgctgttg gcgaaaaggg cgcgccatct ctcgacgggc aagtggatcg tttacactt
//
LOCUS       DASUEX010000490         2300 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_650158, whole genome shotgun sequence.
ACCESSION   DASUEX010000490 DASUEX010000000
VERSION     DASUEX010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_650158"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1478
                     /locus_tag="VFV61_08210"
     CDS             <1..1478
                     /locus_tag="VFV61_08210"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEX4900499.1"
                     /translation="LSDSGAHAIFISTRRLYKHILSVLEKRPLRHVIFFDSEVAEDIE
                     HSIGLEQLEERGRELSEQRPGAYEAHLQAVRPDDLATILYTSGTTGEPKGVMLTHNNF
                     TSNVISIAKGLPITATDVALSVLPLSHIFERDGFYVFCYCGVSVYYAASFDQVGENLR
                     EVAPTVMTAVPRLFEKVYHRIIKKGMSAKGYKKSIFMSSLAVGQRYGELKDKGKWVFP
                     RLALKQKLADKLVFSKWREGVGGRLRYFVSGGAPLSPALSYSYLAAGIPILQGYGATE
                     TCIVSANRPGNNHVGSVGIPFDGIEIKIAEDGEILVRGPNVMRGYYGQPEATAAVLKD
                     GWFYTGDVGHIEKNGHLYITDRKKDLFKLSNGKYVAPQLIESLLKESEFVSQVVVVGT
                     GRKQPVALIVPDWENLREALGDTGPKTHVELSKFPAAIKIVQQDIAKLTSSLIDYERI
                     RRVALLPNEFSIDGGELTPTLKVKRKVIDERYGDLIEELYS"
     gene            complement(1487..1741)
                     /locus_tag="VFV61_08215"
     CDS             complement(1487..1741)
                     /locus_tag="VFV61_08215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900500.1"
                     /translation="MKTSRTVPFRKTAACPASATLVSFRCQNLPAETETLIREHLRIC
                     DFCCAELRLLAHHQPATSKYPTPEIPMNLRILAESILNRG"
     gene            2097..>2300
                     /locus_tag="VFV61_08220"
     CDS             2097..>2300
                     /locus_tag="VFV61_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900501.1"
                     /translation="MKSISEDPEKTADLEPGESRPLPSARRQERGKQAKWVESLKSSD
                     VNRLWTELHRIVSHHPLVRASRSA"
BASE COUNT          534 a          641 c          656 g          469 t
ORIGIN      
        1 tcctttccga ctcgggtgca cacgccattt tcatctccac ccgacggctc tataaacaca
       61 tcctgtcagt gctcgaaaaa cgaccactgc ggcacgtgat cttcttcgac tccgaagtcg
      121 cggaagacat cgagcacagc attggcctcg agcagttgga agagcgcggg cgcgagctgt
      181 ccgagcaacg ccccggagca tacgaagcac acctgcaagc cgtcaggccg gacgatctcg
      241 cgacgatcct ttacacgtca ggcacgacag gcgagcccaa aggcgtgatg ctcacgcaca
      301 acaacttcac gtcgaacgtc atttcgatcg cgaagggatt gccgatcact gcgactgatg
      361 tggcgctgtc agtcctgccg ctatcgcaca tcttcgagcg ggatggcttt tacgtcttct
      421 gttactgcgg cgtgtcggtt tactacgcgg cgtcgttcga tcaggttggg gaaaacttgc
      481 gcgaagtggc gccgacagtg atgactgcgg taccgcggtt gttcgagaag gtctatcacc
      541 gcatcatcaa gaaaggcatg tctgcgaaag gttataagaa gtcgatcttt atgagctcgc
      601 tggcggtggg ccaacgctac ggcgagctga aggacaaggg caagtgggtg tttccgcgtc
      661 tcgcgctcaa gcagaagctc gcggacaagc tcgtgttttc gaagtggcgt gagggtgtcg
      721 gcgggcgctt gcgatacttc gtgtcgggcg gcgcgccgct ctcgccggca ttgtcttact
      781 cgtatctcgc ggcgggcata ccgatcctgc aaggttacgg cgcgacggag acgtgcatcg
      841 tgtcggcaaa ccggcccggg aacaatcatg tcggatcggt gggaattccg tttgacggaa
      901 ttgagatcaa gatcgcggaa gacggcgaaa tcctcgtgcg tgggcccaac gtgatgcgcg
      961 gttactacgg tcagccggag gcgactgcgg ccgtgctcaa ggatggctgg ttttacacag
     1021 gtgacgttgg gcacatcgag aagaacggac atctctacat caccgaccgg aagaaagatc
     1081 tgttcaagct gtcgaacggg aagtatgtcg cgccgcagtt gattgagagc ttgctgaagg
     1141 agagtgaatt tgtcagtcag gtggtggttg ttgggaccgg tcgaaaacag cccgtggcgt
     1201 tgatcgtgcc ggattgggaa aatttgagag aagcgcttgg cgacacgggg ccaaaaaccc
     1261 acgtcgagct cagcaagttc ccggctgcca tcaagatcgt ccagcaggac atcgcgaagc
     1321 tcacttcatc cctcatcgac tacgagcgca tccgtcgcgt ggcgctcttg cctaacgaat
     1381 tcagcatcga cggcggcgaa ctcacgccca cactgaaagt taagcgaaaa gtgatcgacg
     1441 agcgctacgg cgatttaatc gaagaactct attcgtaaat ccgcgttcat ccgcggttaa
     1501 ggatcgactc cgccagaata cgcagattca tcgggatttc cggcgttgga tatttgctgg
     1561 tggccggttg atgatgcgcc agtagtcgca gctccgcaca gcaaaaatcg cagatccgca
     1621 ggtgttctct gattagggtc tccgtctcag ccggcaggtt ctggcagcga aacgagacca
     1681 gtgtcgctga ggcaggacac gcagcggtct tccggaaggg aactgttctt gaggttttca
     1741 ttcaaccatc cctgagattc actcctagca aatacacaat taccctcact ttccagcctt
     1801 ttcttaagga atctcaactt tttaacaggt gtaaaaagat gttgctcgtt ctttaaaatg
     1861 ctgttaccat gccttcgtca gtcagcagcc ataaccggca ctactggcgc atctccagga
     1921 gcaccaaccg cgatccagcg gtaaaaaatc gggcaaaaag agaacttttc taacagcttg
     1981 ctgtcggcga gcacctgtac tttctatgcc gaaataacgg acgcacccaa cccatccgaa
     2041 gctcacaacc cccacccgaa cacgaactcc cagacgagta gggcaaacga caacctgtga
     2101 aatccataag cgaagatcct gaaaagacag cggacctcga gccgggcgaa agtcgcccgc
     2161 tgccgagcgc gcgccgccaa gagcgaggaa agcaggcgaa gtgggtcgag agcctgaagt
     2221 cttccgacgt caatcgtctc tggaccgagc tgcaccgcat agtttcgcat catccgctgg
     2281 tacgcgcgtc ccgcagcgcc
//
LOCUS       DASUEX010000491         2517 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1674082, whole genome shotgun sequence.
ACCESSION   DASUEX010000491 DASUEX010000000
VERSION     DASUEX010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1674082"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1560
                     /locus_tag="VFV61_08225"
     CDS             <1..1560
                     /locus_tag="VFV61_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900502.1"
                     /translation="EKIFWTALQTVLLDSYGIPIKASSYSYRRNLSEYRKSHIVRALS
                     VLSLYQAVGGAPVFSEAEMSDIQSRLLLTGKTFGGLVDDLYLQSYYERQVTALRDERG
                     HNWELLRQRAEAESLVFEPLEMPDGSTTHAMLWIAKDELAAKREQAYDSRFLNISDPR
                     SDNRLKKWDGYVETRFYDAENREVTAGTAGAHPVELIPLALYGLDNPKIPMLLIDFRD
                     SLNPKKREMSRRVLQDLTRNFVSLSGASGMAFFLGRTVFHFATARRGIDINQPSRLRT
                     YSQLKLLLALNNSLEPELKQQIDARLDKVSVNPFENDLAAEVKLAHEQYQALKSYALK
                     TDGLPAKLDRDRREEMVPLKHNKAEQILFRLGNILSFGTYTHREKANSEDESRLAVAR
                     RIAYHTRFLRQVAKSTPRVEVVWNLEEVRRSLQFIADHGAEVGSTATHAAATIFLKTE
                     DDETRRACLDSLAQINNRKANDELLRISQDKNLEPAWKERVLAYLSRPHPDSPVATSQ
                     GKSRTERSGQQ"
     gene            1593..>2517
                     /locus_tag="VFV61_08230"
     CDS             1593..>2517
                     /locus_tag="VFV61_08230"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX4900503.1"
                     /translation="MRNRPVLAHPLRLSILAAVLVIFAASHSFAQAKRLVILKVDGLP
                     YSILDSAVQERNPRTGKSQLPWIEHVFYQRGVRLSNFYVRGMSLSAPSWSLLDTGQHL
                     QVKGNVEFDRYTLHAYDYLNFIPFYFKSAVGSRIDMPGAEVLDSLGLPLLCDAFPHEE
                     RYISFQLYQRGMRFATLRDGLQNRFVKNPRELLDEWTMGLQTRDMFFEQMEKELIQSL
                     ANPKIRYLDLYISNFDHVAHHNRDRQSQMLELQEMDSLVGRLWTAIEKSPLADDTAFV
                     MVSDHGFNTDEKIYSQGYNLVKLVGSRAGGGQ"
BASE COUNT          587 a          673 c          660 g          597 t
ORIGIN      
        1 gaaaagatct tttggaccgc gttgcagacg gttctgcttg attcttacgg cataccaatc
       61 aaagcctcga gctattccta ccggcgcaat ctctcggagt atcgcaagtc gcacattgtg
      121 cgcgcacttt ctgtcctttc gctctatcag gcagtcggcg gtgctccggt gttctctgaa
      181 gccgagatga gcgacattca gtcgcggctg ctactgacgg gtaagacgtt tgggggactg
      241 gttgacgacc tctacctgca aagctactac gaacgacagg ttactgctct gcgcgatgaa
      301 cgcggacaca attgggagtt gttgcggcaa cgtgcagaag cggagtccct ggtgtttgag
      361 cccctggaga tgccggacgg cagcacaacg cacgcgatgt tgtggatcgc caaggacgaa
      421 ctcgcagcaa aacgcgaaca agcttacgat tcgcgctttc tgaacatttc tgacccgcgc
      481 tccgataacc gcttgaaaaa gtgggacggc tatgtcgaaa cccgttttta tgacgctgag
      541 aatcgcgaag tgactgccgg cactgcgggt gcacatccag tagagctgat tccgttggcg
      601 ctttacggtc tggacaatcc caagattccg atgttgctga tcgattttcg cgatagcctt
      661 aatcctaaga agcgcgagat gtcgcggcgc gtcttgcagg atctcacccg caacttcgtc
      721 tcgctttcag gtgcgagcgg aatggcattt tttctgggtc gcactgtttt tcatttcgca
      781 accgcgagac gtgggatcga cattaatcaa ccttcccgcc tgcgaacata ttcgcaacta
      841 aagctactgc tggcgctcaa caattcgctt gagcccgaac ttaagcagca gattgatgcg
      901 cggttggaca aggtttctgt caatccgttt gagaatgatt tggctgccga agtgaaactg
      961 gcccacgagc aatatcaggc tctcaagtct tacgcattaa aaaccgacgg cctgccggct
     1021 aagctcgacc gtgaccgccg tgaggaaatg gtgccgctaa aacacaacaa agcagaacaa
     1081 attctctttc gcctcggcaa catcctctct ttcggaacat atacccatcg tgaaaaggca
     1141 aattccgaag acgagagtcg cctcgcagtc gcccgccgca tcgcttacca cactcgcttc
     1201 ctccggcagg tggctaagtc gacgccccga gtggaagtag tctggaatct cgaagaagtg
     1261 cgacgctctc tacaattcat tgccgaccat ggtgcggagg ttggctcaac ggccacgcat
     1321 gcggcggcga ccatcttctt gaaaacagaa gatgatgaaa cgcggcgcgc gtgcctcgac
     1381 agtctggcgc agataaacaa tcgtaaggcg aatgatgagt tgctgcgcat ctcgcaggac
     1441 aagaatctcg agccggcttg gaaagaacgc gtcctagctt atctgagcag gccgcatcct
     1501 gattcaccag tagcaacttc gcagggtaag tcccgcacag aaaggtccgg acaacagtaa
     1561 ctgtcgctat ggcccggctc tttaatttca gcatgcgcaa cagaccagtg cttgcccatc
     1621 cgctacgatt aagcatcctt gcagccgtat tagtaatttt cgccgcgtcc cactctttcg
     1681 cacaggcaaa gcggttggta attttgaaag tcgacggctt gccttattcc attctcgaca
     1741 gcgccgtgca ggaacgaaat ccccgcaccg gtaagagtca acttccctgg atcgaacatg
     1801 ttttctatca gcgcggagta cgcttgagta atttttatgt tcgcggcatg agcttgtctg
     1861 cgccctcatg gtctctgctg gacactgggc aacacttgca ggtgaaagga aatgtcgaat
     1921 ttgatcgcta cacgcttcac gcttacgatt atttgaattt cattcccttc tattttaaga
     1981 gcgcggtggg ctccagaatt gatatgcccg gcgccgaggt gctcgactcg ttgggactgc
     2041 cgcttctctg cgacgccttt cctcacgaag agcgctacat tagctttcag ctttatcaaa
     2101 gaggaatgag gttcgccacc ctgcgtgatg gtttgcaaaa ccgcttcgtc aagaacccgc
     2161 gcgaacttct cgacgagtgg accatgggtt tacagactcg cgacatgttt tttgaacaaa
     2221 tggagaaaga actgatccag agtctcgcaa acccgaagat tcgttatctc gacctctaca
     2281 ttagcaactt cgatcacgtt gcccatcaca atcgcgaccg gcagtcacag atgcttgagt
     2341 tgcaggagat ggatagttta gtgggtcgtc tctggaccgc tattgagaag agtccactgg
     2401 cggacgatac ggcctttgtg atggtgtctg atcacggatt caatactgat gagaagattt
     2461 atagccaggg ctataacctg gtgaaactgg ttggcagtcg cgctggcggc ggtcagc
//
LOCUS       DASUEX010000492         3661 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_650222, whole genome shotgun sequence.
ACCESSION   DASUEX010000492 DASUEX010000000
VERSION     DASUEX010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3661
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_650222"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="VFV61_08235"
     CDS             complement(<1..393)
                     /locus_tag="VFV61_08235"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEX4900504.1"
                     /translation="MSEIIGVVGAGTMGNGIAQVAARAGYDVVISDVSEEFLQRGLTA
                     IDKSLQRDVDKERLAAAEKIEITRRIQTNTSLESLRDAGLVIEAVTENVNVKTEVFQT
                     LDRIVGADTILASNTSSISITKLAAATKR"
     gene            complement(419..898)
                     /locus_tag="VFV61_08240"
     CDS             complement(419..898)
                     /locus_tag="VFV61_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900505.1"
                     /translation="MTPTGHNRTLGLLHLLYGVFCIALIVLATFLISGAIDAMAVRGE
                     AVPVATFAVLIVGGVFVSLVLITPSFISGYALLKRKRWARRIGIVAGVLAGLTFPFGT
                     ALCVYTLWFMFGEKGKFLYHKAAYALPPQPEPSRRKLKKLLRASEPYVPPTTPPDWR"
     gene            complement(903..2090)
                     /locus_tag="VFV61_08245"
     CDS             complement(903..2090)
                     /locus_tag="VFV61_08245"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEX4900506.1"
                     /translation="MSVIKEAVIIGAVRTPVGKFQGSLKGFKATELGAIVVREVVKRA
                     GVAPDAVDEVIMGCVIQAGLGQNPARQAALNGGLPNTVSAVTVNKVCGSGLKAVMMAA
                     QGIQLGDTDIVVAGGMESMSNAPYLLTKAREGYRLGHGTLVDAMINDGLWCAFDNQHM
                     GCTGEVVAERFHVSRTEQDEYALGSHRKAAAAIKEGRFKDEIVPVEIPQKKGAATVFD
                     TDETVREDTSLEVLGKLKPAFKEGGTVTAGNAPGVNDGASAVVVTSLERANALGIAPM
                     ARIVAQATSGTEPQLVMMAPVEAIKKVLQKANWSTNDVDLIELNEAFAVQAVAITREL
                     NLDPHRINVNGGAVALGHAIGQSGSRLLTTMLFEMKRRDAHRGVVALCLGGGNAVALA
                     VER"
     gene            2349..3392
                     /locus_tag="VFV61_08250"
     CDS             2349..3392
                     /locus_tag="VFV61_08250"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HEX4900507.1"
                     /translation="MQALILAGGKGTRLRPLTVYTPKPIVPICNRPFLLYQIDTLRRA
                     GITDITLSLSYQPNKIEHLLGDGSDYGVNLKYTVEPQPMGTAGAYKFAEDLIRERTVV
                     FNGDILTDLDLKAVIREHKERKATATIVLQPVANPRAYGLVETETDGRVHRFLEKPKE
                     DEITCNNINAGTYILEPAVLDFIPAGENYSFEYALFPDLLMRKEAFYAHVPPRTYWID
                     IGTPERYLEAHQDLLANRVQRIHIKERRGSFDSATVAEIDELSIIGDDCQIKPGAQIV
                     NSVLGAGCFVEERARVENSVVWPHTRIGTAATVSGAIIGRGCHVGRSAVVGAGSVLGD
                     KTSVTDYTITGQA"
BASE COUNT          816 a         1059 c          942 g          844 t
ORIGIN      
        1 ccgtttcgta gctgcggcca gtttcgtgat cgagatcgaa gaagtgtttg aagcgagaat
       61 cgtgtcggcg ccgacgatcc gatcgagtgt ctggaagacc tcggttttca cgttaacgtt
      121 ttctgttacg gcttcaatca caaggcctgc gtcccgtaaa gattcaagcg aagtattcgt
      181 ttggattcgc ctggtgatct cgatcttttc agcggccgct aaccgctcct tatcgacgtc
      241 gcgttggaga cttttatcta ttgcggtcaa gcctcgttgc aggaactctt cactaacatc
      301 gctgatgaca acgtcgtagc cggcgcgcgc ggcaacttga gcaatgccat tacccatcgt
      361 gcccgcaccg accacgccga taatttcgct cacttatctt gactccgttg cagtgggatt
      421 aacgccagtc aggcggtgtc gtcggtggta catagggttc cgacgcgcgc agcagttttt
      481 tcagtttgcg acgactcggt tccggttgcg gcggcagggc gtaagcggcc ttgtgataaa
      541 gaaacttccc tttttcgcca aacataaacc acagcgtgta aacgcaaagc gctgtgccga
      601 agggaaaggt cagaccggca agcacgcccg caacgatgcc aatccgccgc gcccagcgtt
      661 ttcgttttag caaagcgtaa ccgctgatga aggaaggtgt gatcagaacc agactgacga
      721 agactccgcc aacaatcagg acggcgaacg tcgcaaccgg cacagcttca ccgcgaaccg
      781 ccatcgcatc gatggctccg gagatcaaaa acgtcgccag gacaatgagt gcgatgcaaa
      841 acaccccata gagcaggtgc agcaatccca gagttctgtt gtgaccagtc ggggtcatta
      901 ccttatctct ccaccgccag cgccactgcg tttcctccgc caaggcagag tgccaccaca
      961 ccacgatgcg catcgcgccg cttcatctca aacagcattg tcgttaacag ccgcgaccca
     1021 gactgaccga tagcatgtcc aagcgcaacc gcgccgccgt ttacatttat tctgtgcgga
     1081 tcgagattta gctcgcgcgt tatcgccaca gcctgtactg caaaggcctc attcaactca
     1141 atcaagtcga catcattcgt ggaccagttt gctttttgca atactttctt gattgcctct
     1201 accggggcca tcatgacgag ttgcggctcg gttccagaag ttgcttgcgc gacgatacga
     1261 gccattggtg cgatgccgag cgcgttagcg cgctccagcg aagtgacgac cacagccgat
     1321 gctccgtcgt taactccggg agcattaccg gccgtaacgg ttccaccttc tttgaacgcc
     1381 ggctttagct ttcccagcac ctcgagcgaa gtgtcctcgc gaacggtctc gtcagtatca
     1441 aagactgttg ccgcgccttt cttctgcggg atctcaaccg gaacaatctc atctttgaat
     1501 cttccctcct tgatcgccgc cgcagccttg cgatgagatc ccaaggcgta ctcatcctgc
     1561 tcagtacgac taacatggaa gcgttcagcc acgacctccc cggtgcaacc catatgttga
     1621 ttatcgaagg cgcaccagag gccatcgttg atcatcgcat ccaccagagt gccatgcccc
     1681 aggcggtatc cttcgcgcgc tttggtgagc aaatagggag cgttactcat tgactccatg
     1741 ccgccggcga cgacgatgtc cgtgtcgcct aactgaattc cctgcgccgc catcatcact
     1801 gccttcaatc cggagccaca gactttgttc acggtgacag cggaaacggt gttgggaagg
     1861 ccaccgttta atgctgcctg gcgcgcgggg ttctgtccca gtccggcctg aatcacgcag
     1921 cccatgatca cttcgtcaac tgcgtccgga gcgacgcccg ctcgtttcac aacttcacgg
     1981 accacgatgg cgcccagttc ggttgccttg aatcctttaa gcgaaccttg aaactttccc
     2041 acgggggtcc tgactgcccc gataatcaca gcttctttaa taaccgacat tcgctctccc
     2101 attacaaata tgcgctcttg cttactatca cctttgctaa ccatttacta acttaactaa
     2161 aagagtgtca agtgattgcc gattgccgat tggcgattgg aacttgacca acgaactttc
     2221 ctaactgtac tcgtgactgg gcgcaaatgg ctcttattcc gataacaaca atcggcaatc
     2281 ggcattttgc aatcgacaat gttagactcc ccttgttatt taaacccgcg cattagtcgg
     2341 tatcaagcat gcaggctctc attctcgctg gaggcaaagg cacacgcctt cggccgttaa
     2401 cggtttacac accaaaacca atcgtcccca tttgcaatcg tccttttctg ctctaccaga
     2461 tcgatacgct acgtcgtgcc ggaattacgg acatcacact ctcgctctcg tatcagccaa
     2521 ataagattga acatctgttg ggggacggtt ccgactacgg ggtgaacctg aaatacacag
     2581 tcgagccgca gccgatgggg acggcaggcg cttacaagtt tgccgaagat ttgattcgcg
     2641 agcgcacggt cgtttttaac ggcgacattc tcacggatct tgatctcaaa gcggtgatac
     2701 gggaacacaa ggagcggaaa gcgacggcaa cgatcgtgct gcaacccgtc gccaatccgc
     2761 gtgcttacgg tttagtggaa acggaaaccg acggacgggt acaccgcttc ctggaaaagc
     2821 cgaaggaaga cgaaatcacc tgcaacaaca tcaacgccgg tacctatatc ctcgagcccg
     2881 cagtcctcga tttcattcct gccggcgaga actactcgtt tgagtacgca ctctttccgg
     2941 atctactgat gcgcaaagaa gctttttacg cgcatgtgcc gccgcgcact tattggattg
     3001 atatcgggac gcccgagcgt tacctggagg cgcatcagga cctcctggcg aatcgcgtcc
     3061 agcgtatcca catcaaggaa cggcggggat ctttcgattc cgcgacagtt gctgaaattg
     3121 atgaactctc gatcatcggt gacgactgtc agatcaagcc tggcgcccag attgttaact
     3181 cggtattggg agccgggtgt ttcgtcgaag agcgagcgcg ggtcgagaac tctgtggtct
     3241 ggccccacac gcggatcggc actgctgcca ccgtttccgg cgcgatcatc ggccgcggtt
     3301 gccacgttgg ccgttcagcc gtcgtgggcg ccgggtcagt tctaggtgac aagacttcgg
     3361 tgacggacta cacaatcacc gggcaggcat agactcgggt ggaaaggtag aacgttcttt
     3421 tcggcgagta tttctctttc ctcgccgggc ttgagcccgg cgtaatcagc ggcaaattaa
     3481 gaatggaagc cgtttaaacg gcttcttgtt tgaaagggca ggttagcacc gggctgaagc
     3541 ccggtgtgaa tgagagccag tggttcatct gcagatctta tcctttgaga ctcggattat
     3601 tcagcagttc aatgagcact cttcctgaaa tcaagtttgg aacagacggc tggcgagcgc
     3661 t
//
LOCUS       DASUEX010000493         4139 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_421385, whole genome shotgun sequence.
ACCESSION   DASUEX010000493 DASUEX010000000
VERSION     DASUEX010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4139
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421385"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(154..2262)
                     /locus_tag="VFV61_08255"
     CDS             complement(154..2262)
                     /locus_tag="VFV61_08255"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4876 domain-containing protein"
                     /protein_id="HEX4900508.1"
                     /translation="MRFMRLSPTLLGLLVGLVFATPLQAQSRNEGLGVQEGKYRGRLP
                     KSVEVSGPIPAAANYQVAQTTGAITRGANAVTGFDCGVGVPGDDCTAPFVLPFTYTLY
                     DQTFSSVNVSTNGNLQFVSNNPDYGQGDVCFPLEQFNYAILPHWGDLTIGGANEGVFT
                     SVSGSAPNRILNIEWRVSLLGNAPATLNFEVRLYEGLARFDVIYGTAPGGGRDVSVGV
                     QRGTGSTYTEFSCHQSTLRNGMMLIFTGTADTSLFIAGRVTDSDGNPVAGADVNLTGT
                     SSGMVTTDGTGNYIFSGLTSGATYSVAATQAGFNFFPPLRNFGPGFRALTGNFVANFI
                     RTSPPNPGDVLISEFRFRGEDVFTAVNEFVEILNNTNQGITVNVTDGSSGWLVRTADP
                     SINFIIPNGTTIPARGHYLAGNGSGYNLFSYGAADTFYSGDIPDTGGVAIFSTANSGS
                     LDLAHRLDAAGFTGEPNALYREGAGLISPGANAGNYSFVRKLLTGAAQDTNDNAADFQ
                     FVSTDGGIYGGLQSTLGAPGPENTASPIQRNAQIKTALVDPPAGTTNAPNRVRDSTPG
                     VCGGPNCALGTLTIRRRFTNNTGQGVTKLRFRSVDVTTLGNTVAGQAQLRALDSPGGV
                     VTVTGGGTVQVRGLTVEQGGPSGGIPQPLGGGMNTSMAVGVITVGQPLAPGANVNVEF
                     RLGVQTSGIFRFFVNIEALP"
     gene            complement(2428..3861)
                     /locus_tag="VFV61_08260"
     CDS             complement(2428..3861)
                     /locus_tag="VFV61_08260"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX4900509.1"
                     /translation="MSKHLLTTLILCAMLLTFSLPPASAQSVRKRGGQVDLLVLGGTV
                     VTMNQSREVIPDGGIAVKAGKIIAVGSRGDISSKYVGRQTINAAGRIIIPGLINGHTH
                     VPMVLFRGLADDLDLQEWLTKYIFPAEAKNVTEEFVRVGTRLGLAEMIRGGTTTYCDM
                     YYFEDAIAEETAKAGVRGVLGETVIDFPVADNKTNAEAMAYVEKFVKRWQGHELIVAA
                     IAPHAPYTVSEEHLRAARAFSDRTGAPIVTHISETKREVDDSVKAHGASPVAYLDRIG
                     FLGERVIAAHVVWPQGSDTQILKKHSVGVIHNPQSNMKLAAGVAPVPLMMKEGLRVGL
                     GTDGAASNNDLSMWEEMDSAAKLHKVISSDPKVMSAQEAFELATIRGAAALHLEKEIG
                     SLEVGKRADLVLVESDSLNQIPSYNVYSDLVYATKASAVQTVIINGRVVMRNRRLLTL
                     NEKAIKASAQVFRERIVRSLAASPGKE"
     gene            complement(3902..>4139)
                     /locus_tag="VFV61_08265"
     CDS             complement(3902..>4139)
                     /locus_tag="VFV61_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEX4900510.1"
                     /translation="IWKALSEGERDFTELAVVADTETLTPPCGTCRQIIWEFCKHATI
                     VLANLKGQTEVVSIRDLLPRAFDARFLSGGTVHD"
BASE COUNT          951 a         1174 c         1098 g          916 t
ORIGIN      
        1 ggacggacca tgctgttaaa cctcaccggt tcgcgaacac agtttagctc gagaaatata
       61 gggataagag cagagaaaac cgagacgggg agccaaaaga agatagccgg ctgagttctc
      121 ctcagccggc cattcttcta agtggacact ttgttagggc agcgcttcga tgttgacgaa
      181 gaaccggaag atgccgctcg tttggacgcc gagcctgaac tcaacattga cgttcgcccc
      241 aggggccaat ggttgcccca cggtgataac accgacagcc atcgaggtat tcattccgcc
      301 tcccaggggt tgaggaattc ccccagaggg gccaccctgt tccaccgtca gtcccctgac
      361 ttgaactgtg ccgcctcccg taacggttac cacaccacca ggcgaatcca gcgcgcgcaa
      421 ctgagcctgg cccgcgacgg tgttcccgag agtagtgacg tcaacacttc tgaatctaag
      481 tttggtaact ccctgacccg tattgtttgt aaaacgacga cggatcgtca gcgttccaag
      541 tgcgcaattt ggtccgccgc acacgcccgg cgttgagtcg cggacgcggt tgggagcgtt
      601 ggttgttcca gcgggtggat ctacaagcgc cgtcttgatt tgagcgttgc gctgaatcgg
      661 actcgccgtg ttctcgggac ccggcgctcc aagggtcgac tgcaagccgc cataaatacc
      721 accgtcagtt gagacaaatt ggaagtctgc tgcattgtcg ttcgtgtctt gcgccgcgcc
      781 ggtgagcagt ttgcgcacaa aactgtagtt ccccgcgttc gcacccggcg agattaagcc
      841 ggctccttcc cggtagagcg catttggttc ccccgtgaaa ccggcagcat caagccgatg
      901 ggcaaggtcg agcgaaccag agttggctgt ggaaaagatc gcaaccccac ctgtatcagg
      961 aatgtcacca ctatagaagg tgtcggcggc gccatatgag aagaggttgt agccgctgcc
     1021 attccccgcg aggtagtggc cgcgggcagg aatggtagtg ccgttaggga taatgaaatt
     1081 gatagaggga tcggcagtcc tgaccagcca acctgatgaa ccgtccgtga cgtttaccgt
     1141 aattccctgg ttagtgttgt taaggatctc gacgaactca ttgacagccg tgaacacgtc
     1201 ttcacccctg aagcgaaatt cgctgatcag aacatcgccg ggattaggcg gagaggtacg
     1261 aatgaaattg gcaacaaaat tccccgttaa ggcgcggaag ccagggccga agtttcgcag
     1321 cggcgggaag aagttaaaac ctgcctgcgt cgcggcaact gaataggttg ctccggaagt
     1381 tagtcccgag aagatgtagt tgccggtacc gtctgttgta accatgccgc tcgaagtacc
     1441 tgtcagattc acgtcggctc ccgctacagg gttgccatcg gaatcggtga cgcgcccggc
     1501 aatgaacaga gacgtatcag cagtgccggt gaagatgagc atcattccgt tacgcaacgt
     1561 actctggtga caggagaatt cggtataagt agagcccgta cctctttgca ctccaaccga
     1621 gacgtcgcgg ccaccaccgg gcgctgtacc atagatcacg tcgaaacgcg ccagtccttc
     1681 ataaagacgg acctcaaaat ttagggtcgc cggagcatta ccgagaagtg aaacgcgcca
     1741 ctcgatgtta agaatcctgt tgggggcact gccgcttacc gaggtaaaaa ctccttcgtt
     1801 tgcaccgcca atagtcaggt cgccccaatg tggcaaaatc gcatagttga attgctcaag
     1861 aggaaaacaa acatcgccct ggccataatc gggattgtta ctcacaaatt gtagattacc
     1921 gttggtgctc acgttaacgc tggaaaacgt ctgatcataa agcgtgtatg taaagggtag
     1981 gacgaacggc gctgtacaat catcaccggg cacaccaact ccacaatcga atccagttac
     2041 tgcgttggcg ccgcgcgtga tcgcaccggt ggtttgcgcc acttgataat tcgcagcggc
     2101 tggaattggt cctgagacct caacagactt cggcagtcgc cccctgtatt tgccctcttg
     2161 caccccgaga ccctcatttc tcgattgcgc ctgaagcggc gtcgcaaaaa ctaagccgac
     2221 cagcagccct aagagcgtcg gagaaagtct cataaaacgc attcgtgatc tcctgaagag
     2281 ttttgatcgt ttactgtttt tggaaactgt cgaacagtgt gtgtactgtt gaccgtatca
     2341 gatgcgcgag cgccggggtc agcaaaagat aacagaggca ctgaggccgg tcaaagcatt
     2401 agttataacc tctgcaccgc gaggccgcta ctccttgccg ggtgaggcag ccaagctcct
     2461 gacgattcgt tcccgaaaga cctgggcgct ggctttgatc gctttttcgt tcagggtcag
     2521 cagacgccga ttgcgcatca cgacacgtcc gttgatgatt accgtctgca ccgccgaggc
     2581 tttcgtcgca tacaccagat cagaatagac attgtaagaa ggaatttgat ttagcgaatc
     2641 gctctctacg agaaccaagt cggcgcgctt gcccacttcc aacgatccaa tctccttttc
     2701 caggtgcagg gcggctgcgc cccgaattgt cgccagctca aacgcttcct gcgccgacat
     2761 cacttttgga tcgctgctga tcaccttatg cagtttggcc gcggaatcca tttcttccca
     2821 catgctcaga tcattgttcg acgccgctcc atccgtgccc agaccgactc gcagaccctc
     2881 tttcatcatc agcggcacgg gagcaacacc cgcggccagt ttcatgtttg actgcgggtt
     2941 atgaatcact ccgacgctgt gcttctttag gatttgcgtg tctgatcctt gtggccaaac
     3001 aacgtgcgcc gcgatcactc gttcaccgag aaagccaata cgatcaaggt aagcgaccgg
     3061 actcgcacca tgggccttaa cgctgtcatc aacttcgcgc ttagtttcag aaatgtgcgt
     3121 gacaataggg gctccagtcc gatcagaaaa ggcgcgggcg gcccgcagat gttcctccga
     3181 gactgtataa ggtgcatggg gagctatggc ggcgacaata agctcgtggc cctgccaacg
     3241 cttcacaaac ttttcaacat aggccatggc ctcggcgttg gtcttgttat cagcgaccgg
     3301 gaaatcaatc actgtttcac caagcacgcc ccgaacaccg gctttcgctg tttcctccgc
     3361 aatcgcatct tcgaagtaat acatgtcgca gtacgtcgtg gtaccgccac gaatcatttc
     3421 cgccaatccc aatcgtgttc ccacacgcac aaattcctcg gtgacgtttt ttgcctccgc
     3481 gggaaagatg tacttcgtca gccattcctg aagatcaaga tcgtctgcca accctcgaaa
     3541 cagcaccatc ggcacatgcg tgtggccgtt tatcaacccg gggatgatga tccgtccagc
     3601 agcgttgatg gtctgccggc caacatattt gctgctgatg tcgccacgtg atccaacagc
     3661 aatgatcttg cctgccttca ccgcgatgcc accatccgga ataacttcac ggctctggtt
     3721 catggtcacc acggttccgc ccaaaaccag caagtctacc tgccccccgc gctttcgcac
     3781 tgactgagcg gaagcggggg gcaatgaaaa ggtcagtagc atggcgcaaa gtatgagcgt
     3841 ggtcagcaga tgttttgaca ttaggagtct gatcaaagtt gggttccctg ctagaagtgg
     3901 cttagtcgtg gaccgtgccg ccactcagaa atctggcgtc gaatgctcga ggcaagaggt
     3961 cccggattga aactacttcc gtttgccctt tcaggttcgc caggacaatc gttgcgtgct
     4021 tacaaaactc ccagatgatt tgccggcagg tgccacacgg cggtgttaac gtctcagtat
     4081 cggcgaccac tgctaattcc gtgaagtctc gttccccttc cgacaaggcc ttccagatt
//
LOCUS       DASUEX010000494         2923 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1132813, whole genome shotgun sequence.
ACCESSION   DASUEX010000494 DASUEX010000000
VERSION     DASUEX010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2923
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1132813"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="VFV61_08270"
     CDS             complement(<1..338)
                     /locus_tag="VFV61_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900511.1"
                     /translation="MKLLLLLPLLFGACTSAQVPRVAQTNVSTPAPERTPDETFVRDI
                     DRLSKIAGLSPLRSSNLSQGDLEARVWYGFGLILLEGFTIKQTNNQWSAFHLKADEAN
                     PRYITKVARI"
     gene            complement(386..781)
                     /locus_tag="VFV61_08275"
     CDS             complement(386..781)
                     /locus_tag="VFV61_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900512.1"
                     /translation="MFTFSPFDVIGQNSCGVLKAAIGLGVACAKRTRKTANWSSNFSD
                     AAHTLGATLEARNRLACVARGQLLLRATAGAPADFGVSANWKYCQILGQKLIARLACL
                     VHNAAQSNNSLDRSGGGVFRIKPGAARVE"
     gene            780..920
                     /locus_tag="VFV61_08280"
     CDS             780..920
                     /locus_tag="VFV61_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900513.1"
                     /translation="MIYRSGTGSINPECDDCFLDAITCYVTIDHFLKRHPFLPVTRFA
                     IV"
     gene            1169..2344
                     /locus_tag="VFV61_08285"
     CDS             1169..2344
                     /locus_tag="VFV61_08285"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEX4900514.1"
                     /translation="MTVAMQVSVYAQLFVPREYEVFAGLDVDHHSIAVTFTDHGRLRQ
                     SLRLPYSAPQLLNYVQKHFPGQRLAFAYEAGPSGFGLYDELLAAGHPCLVVAPSMVPT
                     APGARVKTNRLDSKKLSTALRGGELKSIHVPSSKYRELRHLVQLRDTQVQQLTATKCR
                     IKALLLYEGIPFPSADGKEKLWSAGVLDELLALPCSTAVRFKLDQLIGTLHFNFNAAA
                     ATQKQIRHYCNHDAELRQAVAWLKSLPGIGEIVATHLVARLGDPALITNVRQIAGFLG
                     LVSSEHSTGDKQNRAEITRAGDSRLRNKLIQSAWVTIRKDPELRAFYRRVYQRHPKKV
                     AARKAIVAVARKLTTRIYAVLKEQRPFVLREVNEAPLTTEETAGPRERLDGQQNEQR"
     gene            complement(2612..>2923)
                     /locus_tag="VFV61_08290"
     CDS             complement(2612..>2923)
                     /locus_tag="VFV61_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900515.1"
                     /translation="DVSEFQRLKAVNNQIVVTKSTPVPNREFLEDDAMRSFDSRYREL
                     PEYHDDSYSIYLETTLGPYECIYPAVVARECEGVRQKLDSLFSPYSSDNYRRTLSRDL
                     E"
BASE COUNT          679 a          771 c          831 g          642 t
ORIGIN      
        1 tgaattcgcg cgaccttggt aatgtaacgg ggattagctt cgtcggcctt gagatggaaa
       61 gcgctccact ggttgttggt ttgtttgatg gtgaagccct cgaggagaat taggccaaac
      121 ccataccaga cgcgcgcctc aaggtcgcct tgagaaagat tcgacgatcg caaaggcgac
      181 agccccgcga tttttgatag gcggtcgatg tcgcgcacga aagtttcatc tggagttcgt
      241 tcaggtgcag gtgttgacac gttcgtttgc gcaacgcgag gaacttgcgc cgacgtgcac
      301 gccccgaaca gcaatggaag taggagcaag agtttcatta tcaagaggag atgccgaacg
      361 gtggagttga ccgggcggcg cgatttcatt ctaccctcgc cgcaccaggt ttgatgcgaa
      421 acacgccacc gccgctccgg tccaacgagt tgttcgattg cgccgcatta tgaaccaggc
      481 aagcaagtcg ggcaattagt ttttgcccca ggatttggca atatttccaa tttgccgaga
      541 cgccgaaatc ggccggagcg ccagcggtag cgcgcaacag taattgaccg cgggcaacac
      601 aagccaaccg gttccgtgct tccaaggttg cccccaatgt gtgtgccgcg tccgaaaagt
      661 tcgacgacca attcgccgtc ttgcgcgtac gcttggcgca agccacccca agcccgatcg
      721 ccgccttcaa aaccccacaa gaattttggc cgattacgtc gaagggtgaa aatgtaaaca
      781 tgatctaccg cagcggaacc ggcagtatta accccgagtg tgacgattgc ttcctcgacg
      841 ccatcacctg ttacgtcacc atagaccact ttttgaagcg tcatcccttc ttgccagtca
      901 ccagatttgc catcgtgtag ggtgaaggat ctcgttgggt attcggccgt aggaaacaga
      961 ccaatgctgc catgataacg aaggttagcg aagtcgattg tgcggatagg tggcacagtc
     1021 cgctgtgcga agccgcccga tgaaagcaaa acgattatca gcatcaattg tggcatgaaa
     1081 gtggaagcaa tcgaacgtct gagacatgta tgttgaatgt cagggtcggg tctctagaat
     1141 gagtcgaagg ctcaggagga caacgacgat gacagttgca atgcaagtct cagtttacgc
     1201 tcaactgttt gtgccgcgcg agtacgaggt tttcgccggg ctcgatgtgg atcatcatag
     1261 catcgcggtt acttttactg atcacggtag attgaggcaa tcgctgcgtc tgccctatag
     1321 tgcgccgcag ttgctgaact atgtgcaaaa gcactttccc ggacaacgac tggcgtttgc
     1381 ctacgaagct gggcccagcg ggtttggact ctacgatgag ttgctggccg cgggccatcc
     1441 ctgcttagta gtggcgccct cgatggtgcc gacggccccg ggagcacgag tgaaaaccaa
     1501 ccgactcgac agtaagaaac tctcgactgc cttacgtggc ggcgaactga aaagcattca
     1561 tgtgccgtca tcaaagtacc gtgaactgcg acacctggtg caactgcgcg acacgcaggt
     1621 gcagcaattg acggccacca aatgccgtat taaggcgctg ctgctctatg agggaatacc
     1681 ctttccctca gccgatggta aagagaagct gtggtcagcg ggagtgctgg atgagttgct
     1741 ggccctgccc tgctcgacag cggtgcgctt caagttggac cagctcattg gcaccttgca
     1801 tttcaacttc aacgcggccg cggcgacgca aaaacagatt cgtcactact gcaaccacga
     1861 tgcggaactg cggcaagctg tagcctggct caagagcctg cccggcattg gcgagatcgt
     1921 ggccacgcat ttggtggcac ggcttggtga tccagcgctc atcaccaatg tacgccagat
     1981 tgccggcttt ctgggcctgg tctcgagcga gcattcgacc ggcgacaaac agaatcgcgc
     2041 tgagattaca cgcgcgggtg acagtcggct gcgcaataag ttgattcaaa gcgcctgggt
     2101 gacgattaga aaagatccgg aactgcgtgc gttctaccgc cgggtctatc agcggcaccc
     2161 taagaaagtc gccgcgcgca aagctattgt ggccgtagcg cgcaagttga cgacgcgcat
     2221 ctatgcggtc ttgaaagagc aacgtccatt tgtgttacga gaagttaatg aagctccttt
     2281 aaccacagag gagaccgctg gcccaaggga acgcctcgac ggtcaacaga acgaacagcg
     2341 ttagctgaaa gattcgtgct tgagacagag gacccgggct ttcggtgttc gccctcaaga
     2401 gggccgcatc cacgagagtg tgacgaccgt tgagtcgtgc cacgaatagc agtcagtctc
     2461 tcttgctggt tgaggatgga ttttgatctg gacattcaca tagcagttga ccgggcggcg
     2521 cgtattaacg caaccttcgc cggactaagt tagttgcgaa acacgctccc gccgctccgg
     2581 tccaacgagt tgttggattg cgtctgtagg gctattcaag gtcgcggctc aacgttctcc
     2641 gatagttgtc cgatgaatat ggactgaaca acgaatctag tttctggcga acaccttcgc
     2701 attccctggc gaccacggct gggtagatac attcgtaggg gcccagcgtt gtttcaagat
     2761 aaatactgta cgagtcatcg tgatattccg gtaattcgcg atagcgtgag tcaaacgagc
     2821 gcatcgcatc atcttcgagg aattcgcggt taggaactgg agtactcttc gttaccacga
     2881 tctgattgtt tacggccttg agccgctgga attcggaaac gtc
//
LOCUS       DASUEX010000495         2101 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_650618, whole genome shotgun sequence.
ACCESSION   DASUEX010000495 DASUEX010000000
VERSION     DASUEX010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_650618"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(104..1129)
                     /locus_tag="VFV61_08295"
     CDS             complement(104..1129)
                     /locus_tag="VFV61_08295"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEX4900516.1"
                     /translation="MLQEIGEQPEALARTIESESKKISQLGAFLRKRDIDLIVLVARG
                     SSDNAALFGRYLLEITTGIPVSLSAPSVHTLYHAKLRLEHALVVGVSQSGEGEDINIV
                     LENARASGAYTLGITNESDSTMTGVVEETLLTQGGRERSVAATKTFTGQMMLFYMLAA
                     ELSDGSSKHRFELIPDFAAKALEQKAALRELVQRYVFMENCVVVGRGLAYANAYELAL
                     KLMETCYVVAERFSSADFLHGPLAMIERHFPVILFAPPGVMLAGVKSLIERLCELHAD
                     VLAITSDLDAAGACSRAIIMPKEIDEFLAPIPYIIPGQLFAALLAEAKGLDPDSPRSL
                     SKVTRTL"
     gene            1381..>2101
                     /locus_tag="VFV61_08300"
     CDS             1381..>2101
                     /locus_tag="VFV61_08300"
                     /inference="COORDINATES: protein motif:HMM:NF014852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEX4900517.1"
                     /translation="MSNTLARRGLVRLAAVVLIVVAAGSSTLSQESVQIQIIPAPQQL
                     TIKSGNFKIRRATRIALADSTSKDDRFAAEDFAGDLKESAKVDLKITKGRSSNSILIG
                     KLSLPIIQATLKQMAIPVPPQLDPEGYVLAVSDKQVIIAGASEAGTFYGLQTLKQLVR
                     GEGVDASIAGVEIVDWPAMRWRGVSDDVSRGPVPTVEYIKRQLRTFAAFKLNLHSFYM
                     EQTFGGTAHPLIGPEGGSLTPS"
BASE COUNT          512 a          568 c          541 g          480 t
ORIGIN      
        1 aaggcctcaa cctgttggcg cacctgcgca taaacaggac catggctggg gtctttcaaa
       61 gcaatattca tgatcgaaat ctaccttgtg tgtttcgggt cactcaaaga gttcgcgtca
      121 cctttgataa cgacctgggc gagtcgggat ccagtccctt tgcctcggca agcagcgcag
      181 cgaacagttg accgggaata atgtaaggaa tcggagcaag gaattcgtcg atctctttgg
      241 gcatgataat cgcacgagaa catgctccgg ctgcgtccag gtcactggta atcgcaagca
      301 catcggcatg aagctcgcat aaccgttcaa tcagactctt cacacctgcc agcatcacgc
      361 ctggaggcgc aaaaaggatt acgggaaagt gtcgctcaat catcgccagc ggcccatgca
      421 gaaagtccgc cgaagagaag cgctccgcta cgacataaca cgtctccatt agtttcagcg
      481 ccaattcata agcgtttgca taggcaagcc cgcgtcccac caccacacag ttctccatga
      541 agacataacg ctgaaccaac tcgcgcaggg ctgctttctg ttcgagggcc ttcgccgcaa
      601 agtctggaat gagttcgaat cgatgtttgg atgagccatc tgagagctcg gcagcaagca
      661 tgtagaagag catcatctga ccggtgaacg ttttggtggc ggcaaccgat cgttcacgcc
      721 caccctgtgt caacagagtc tcctctacca cccctgtcat cgtcgaatct gattcattcg
      781 taatgccgag cgtgtatgcg cccgatgcgc gggcgttttc cagcacgata ttgatgtcct
      841 caccttcgcc tgattgcgaa acgccaacca cgagtgcgtg ctcaagccgg agttttgcat
      901 ggtagagcgt gtgtactgat ggggcggaaa gtgagaccgg aattccggtt gtgatctcaa
      961 gaagatagcg accaaagagt gcagcgttat cagaactccc acgggcgacc aggacaatta
     1021 gatcaatatc gcgtttgcga aggaaagctc cgagctgaga gatcttcttt gattcagact
     1081 caattgtgcg ggccagtgct tccggctgtt cgccaatttc ctgaagcatt aaagacaagg
     1141 tttagcctcc caaggtctca agcgacagat gccccggcga ccttcaacgc gactcattgt
     1201 aaacaagcgt ggatttctgt taaataggcg gccgttcttc aagttcaaat ccatttgaaa
     1261 ctgagccggt ccgaaccatt gatcccgaaa tcacgcggga ctgagatgaa cacgcatcgg
     1321 tccctcccgc aatttcgcgg atcactgtct acgtcatcaa tatttatacc aggcgaagtt
     1381 atgtcaaaca ctctagcacg aagaggctta gtgcgtctgg ccgcggttgt tttgatcgtt
     1441 gtcgccgctg gttcatcaac actttcacag gaatccgttc agatacaaat catacctgct
     1501 ccccaacaac tcacgatcaa gtctggtaat ttcaagatcc ggcgagccac tcgcatcgcg
     1561 ttagctgact cgacctcaaa ggacgatcgt ttcgccgctg aggatttcgc cggcgatctg
     1621 aaagagtcag caaaagtaga tctcaaaata actaaaggac gttcgtccaa ctcaatcctg
     1681 atcggcaaac tcagcctccc gattattcag gcgacactga agcagatggc cattcctgtg
     1741 ccgccgcaac tcgatccaga aggctatgtt ctggctgtga gcgacaaaca ggtgatcatc
     1801 gcaggcgcga gcgaagccgg tactttttat gggctgcaga ccttaaagca acttgtgcgc
     1861 ggcgagggcg ttgacgcgag cattgctggg gttgagatcg ttgattggcc ggcaatgcgc
     1921 tggcgtggag tgtctgacga tgtgagtcga ggtccagtgc caaccgttga gtacatcaag
     1981 cgccaacttc gcacgtttgc tgcctttaag ttgaacctgc attcgttcta catggaacag
     2041 acatttggcg gcaccgcgca tccattgatt ggccctgagg gtggctcgct tacaccttcc
     2101 g
//
LOCUS       DASUEX010000496         4121 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1675080, whole genome shotgun sequence.
ACCESSION   DASUEX010000496 DASUEX010000000
VERSION     DASUEX010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4121
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1675080"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(291..1319)
                     /locus_tag="VFV61_08305"
     CDS             complement(291..1319)
                     /locus_tag="VFV61_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900518.1"
                     /translation="MKELYKNYMHGRERAYYERDNNRLVRPFEWGLPFIVDRVNGDDP
                     RHVLNRHTENVLQNSDAFYYLPPISDFQLAGDRLTWTSAVQTDSAENNIANARFFPAK
                     ADDRKPKRAVVVLPQWNADLHSHVEACRIFNFLGISALRLTLPYHEDRRPPELERADH
                     LVGPNIGRTIQSFRQGVLDTRAAVQWLSEQGYERIGILGTSIGSCISFLAFAHEPRIV
                     AGTFNHVSGYVADVAWKGISTQHVFEGLEPHVTLDELRNYWRPISPVPYIGRLQQLGH
                     RPMRFIAARYDLTFPLDLTHDVIDEVKKHGLPLDVVWLACGHYTTAEMPWKAIDAWKI
                     ATFHRKHL"
     gene            complement(1353..1916)
                     /locus_tag="VFV61_08310"
     CDS             complement(1353..1916)
                     /locus_tag="VFV61_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900519.1"
                     /translation="MMSKELAAPIGDPSKHSYIGTAPMATFLRQSAGFHWNFVVLTLF
                     LLLSSAFPQLTGNVVRASTPSYSVKAKRSAELRSLMKKLRGQGARVALTSERIKQPFF
                     SVPARILNIDGVSVQVFQYSLLSAASKEAAVVNSDGMTIGTSKPSWMAPPHFFRSGRL
                     IVLYVGNEQSILMLLQGALGNQFAGNE"
     gene            2033..2890
                     /locus_tag="VFV61_08315"
     CDS             2033..2890
                     /locus_tag="VFV61_08315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900520.1"
                     /translation="MFKASHRTALAAILGLFVFAALPSATSADHSWGGYHWARTSNPF
                     TIKTGDNVTSAWDAYLDEAITDWNVSNVLDLLKVAGGTNPKNCRPTSGRIEVCNSKYG
                     NTGWLGIAQIWITGGTHITQAITKVNDTYFNTSQYNTPAWRRLVMCQELAHDFGLDHQ
                     DVTFNNFNLGSCMDYTNAPAGGVVGGFNYGPSNEHPNAHDYEQIEIIYQHLDSTTTIG
                     QSVVSRGNGQTPPAMNDLEMDGPGQWGRLVSSSRNGRIQVFELDFGNGNKILTHVFWA
                     SSEEREEDH"
     gene            complement(3012..>4121)
                     /locus_tag="VFV61_08320"
     CDS             complement(3012..>4121)
                     /locus_tag="VFV61_08320"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX4900521.1"
                     /translation="EIVMALVSGNSVVLKPSELTTLTALKLGEVFRDSGLPPGLLEIV
                     TGDGAVGASLIDARVDKIMFTGSVATGKRVAAAAAKHLTPVVLELGGKDPMIVLEDAD
                     IPNAARGAVWGAFANSGQACASVERCYVHEAVAPEFLARVLAETATLKQGVGTAADTD
                     VGAMTNERQLEIVEDHVADAVAQGANVLSGGKRVTNSAGFFYPPTVLTNVNHGMKIMR
                     DETFGPVLPIMTFKTEAEAVELANDSVFGLTASVWTNNIGRGRRLAEQIAAGTVMVNE
                     VLYTHGIAQTPWGGVKESGYGRTHGRAGLLELVNPHHIHVNRFGWLPDLWWFRYDQSA
                     GKLFRGLANYFTTGSIIRTSRLLPEMLRRLLKLRK"
BASE COUNT          968 a         1147 c         1069 g          937 t
ORIGIN      
        1 gctgaatgga tcttgtaggc aagggttaga aaagagtccg cgacggacgg gagcataaag
       61 cctgggccgt aagccccagg ttaacagtca acaaatgttt gaacccgcgc agcgggtgac
      121 agccttcgat tctctatcgc ccgcgtcggg ggctcgtaaa ctgatccccg cttggtgaga
      181 attcgttagg ctccattcta ttcaggttga gtagctggat ctctagcggt agcacgctta
      241 gcttgccccg ttcccaacct gcgaatttcg tagcgggctg cgttatctct ctataagtgc
      301 ttccggtgaa acgtagcaat cttccacgcg tcgatcgcct tccagggcat ttccgcggtg
      361 gtgtaatgac cgcaagccag ccagaccacg tcgagtggca gcccatgctt tttgacctcg
      421 tcgatcacat cgtgcgttag gtcgagcggg aaagtaagat catagcgcgc ggcgataaat
      481 cgcatcgggc gatgtcccaa ctgctgcagt cgcccgatgt aaggcacagg actgataggc
      541 cgccagtagt tgcgtagttc atcaagcgtc acatgtggct cgagaccttc gaacacatgt
      601 tgcgtggaaa ttcccttcca ggcgacatcg gcgacataac cggaaacgtg attgaaagta
      661 ccggcgacga tgcgcggctc gtgggcgaac gccaaaaacg atatacacga accgatgctg
      721 gttcccagaa tgccgattcg ttcgtagcct tgctcactta gccattgcac cgccgcgcgt
      781 gtatcgagca cgccctggcg aaacgattga atcgtccgac ctatatttgg acccacaaga
      841 tggtcggccc tctcgagctc aggtggccgg cggtcttcgt gataaggaag cgtcagacgt
      901 aacgcggaga tgccgaggaa gttgaagatg cgacaagctt cgacgtgact gtggagatcg
      961 gcattccatt gcggtagcac tacaactgct cttttcggtt tccggtcgtc ggccttggcg
     1021 ggaaagaatc gcgcgttggc gatgttgttt tcggctgagt cagtttggac ggcgctggtc
     1081 caggtcaacc gatctccggc gagttgaaaa tcgctaatcg gtggcaagta atagaacgca
     1141 tcgctgtttt gcaggacgtt ttcggtgtgc cggttgagta cgtggcgggg atcgtcacca
     1201 ttaacgcgat cgacaatgaa gggcaagccc cactcgaatg gtcggaccag ccggttattg
     1261 tcgcgctcgt agtaggcacg ctcgcggccg tgcatgtagt ttttgtagag ttctttcatt
     1321 tccagttatt ggcgccgcac atttcctcta gcctattcat ttccggcaaa ctggttacca
     1381 agcgcgcctt gtaacagcat tagaatcgat tgctcgtttc cgacataaag tacgatcaat
     1441 cgcccactcc tgaaaaagtg cggtggggcc atccacgaag gtttgctcgt gccgatagtc
     1501 ataccatcgg agttgaccac cgctgcttct ttgcttgccg ccgaaagcaa cgaatattga
     1561 aatacctgaa cgctcacacc gtctatattt aaaatccgcg ccggaacaga aaagaagggt
     1621 tgcttaattc tttcacttgt cagtgcaact ctcgccccct ggccacggag cttcttcatc
     1681 agacttcgaa gctctgcaga tcttttcgcc ttgactgaat aagaaggtgt actcgcccgg
     1741 acaacgttac cagttaactg cgggaaagcc gaagacagaa gaagaaatag cgtcaggaca
     1801 acgaaattcc agtgaaaacc agcgctttgt ctgagaaaag tggccatagg tgccgtgcca
     1861 atataactgt gtttacttgg gtctccaatt ggggcggcaa gctcttttga catcatccgg
     1921 caatgggaat acattggcgt cgttctttac atctcctgct tccgtttcac gtgggaaacg
     1981 cactcgaagc aggagctcag acctaaactt caccaaccgg agaaaaagac taatgttcaa
     2041 agcttcacat cgaactgcgc tcgcagcaat tctcggctta tttgtttttg ctgcgctccc
     2101 gtctgcaaca tccgcagacc attcctgggg gggctatcat tgggcccgca cctccaaccc
     2161 tttcacaata aaaaccggag acaacgtaac gtctgcctgg gatgcatacc tcgatgaggc
     2221 aattactgac tggaatgtat cgaacgtcct cgacctcctc aaagttgccg gcggaacgaa
     2281 ccctaaaaac tgccgaccga ccagcggtcg catcgaagtc tgcaactcaa aatacggcaa
     2341 caccggttgg ctgggtattg ctcagatttg gatcaccggt ggcacccaca tcacgcaggc
     2401 aattaccaag gtgaacgata cctatttcaa tacctcgcaa tacaacaccc ccgcgtggag
     2461 acgcctggtg atgtgtcagg aactcgctca tgatttcggt ctcgaccatc aggatgtaac
     2521 gttcaataac tttaatctcg gttcgtgcat ggactacacc aacgctccgg ctggcggagt
     2581 cgttggcgga ttcaattatg gccccagcaa cgagcatcct aacgcacacg actacgagca
     2641 aatcgaaatc atctaccaac acctggacag cacaactacc attggccaga gcgtcgttag
     2701 tcgcggtaac ggccagaccc ctcccgcgat gaatgacttg gaaatggacg gaccgggtca
     2761 gtggggtaga ttggtcagtt cctcacgcaa tggacggatc caggtcttcg aactcgactt
     2821 cggaaatggg aacaagattc tcacgcacgt cttctgggcg agcagcgaag agagagaaga
     2881 ggatcactga aaactattag acattatctc agcgagggtg tctttttagt tgagggcgag
     2941 atttaattct cgcccttttc ttttgccctc cctatcaacc gtctttgacc tttgaagggt
     3001 gggacagaac tttacttccg cagctttaac agtcggcgca gcatttcagg aaggaggcgt
     3061 gaagtgcgga tgatcgaccc ggtagtgaag taattggcga gaccacgaaa gagttttccc
     3121 gcgctttggt cgtaacggaa ccaccaaaga tccggtagcc acccaaatcg atttacgtga
     3181 atgtgatgag gattaacaag ttcgagtaat ccggcgcgac cgtgcgtacg cccgtaacca
     3241 ctctccttaa ctccacccca gggcgtttgc gcgatgccgt gcgtgtagag cacttcattt
     3301 accatcaccg tgccggcggc aatctgttca gcgaggcgac gtccacgccc gatattgtta
     3361 gtccagacac tcgcggttaa tccgaagacg ctgtcgttgg ctaactccac ggcctcagct
     3421 tccgttttga aagtcattat cggcaacacg ggtccgaagg tctcgtcgcg catgatcttc
     3481 atgccgtgat ttacattcgt gagcaccgtc ggcggataga agaagccggc cgagttggta
     3541 acacgtttgc cgccgctcaa cacattggcg ccctgcgcca cggcgtcggc tacatgatcc
     3601 tcgacaatct caagttgtcg ctcattagtc atcgccccaa catcggtatc agcagcagtg
     3661 cccacaccct gcttcaacgt cgctgtctca gccagtacgc gggctagaaa ttctggcgcc
     3721 acggcttcgt gtacatagca acgttccaca gacgcacatg cctgacctga gttagcaaac
     3781 gcgccccaaa ctgcgccgcg tgccgcgttg ggaatgtccg cgtcttccaa aacaatcatt
     3841 ggatccttgc cacccaactc cagcacaacc ggtgtcaaat gtttggccgc tgccgcggcc
     3901 acgcgtttcc ctgtcgccac gctgccggtg aacatgatct tgtccacgcg cgcatctatt
     3961 aaggacgcac ccacagcgcc atcgccggtt acgatctcca acaaacccgg cggcaaaccg
     4021 gaatcacgaa agacttcacc gagtttcagg gccgtgagcg ttgttagttc gctcggtttc
     4081 agaaccacgg agttaccgga aactagcgcc atcacgattt c
//
LOCUS       DASUEX010000497         3651 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_305985, whole genome shotgun sequence.
ACCESSION   DASUEX010000497 DASUEX010000000
VERSION     DASUEX010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3651
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305985"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..603
                     /locus_tag="VFV61_08325"
     CDS             <1..603
                     /locus_tag="VFV61_08325"
                     /inference="COORDINATES: protein motif:HMM:NF014003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HEX4900522.1"
                     /translation="VHVAVVDPGVGSSRRGIVVSAAEQIFVGPDNGIFSYIYERETNL
                     KVYELSNAKFFRKPVSPTFHGRDIFAPVAAALTNGVRLASLGKPVNDPVRLASLTPER
                     RGSRLLARIIHIDRFGNCVTNLTADLLTPEMIEAGAVLISKRKTIRSFRKFYEEETAA
                     PEKVFCIWGSAGFLEIAAMNTSAAKLLGAERGGRVIVRRS"
     gene            821..1966
                     /locus_tag="VFV61_08330"
     CDS             821..1966
                     /locus_tag="VFV61_08330"
                     /inference="COORDINATES: protein motif:HMM:TIGR01800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HEX4900523.1"
                     /translation="MSDKANAPTAPSAAGLRGVAAATSSMSDVIGDKGQLIYQGYDIH
                     DLAAHSTFEEVIFLLWNKRLPKQTELDELKQALASEYEVPPQIIDLMKRIPRDADPID
                     VLRTTVSALEFYDPRSRDISRAASVRTAVKLTAQFPTLVATAERLRKGLDPVKPNPQL
                     NIASNFLYMLNGSAPSEADARMFDIALILHADHELNASTFAARVVAGTLADMYGAITA
                     AIAALSGPLHGGANTAVMKTLLEIGEVANVESFIKQALADKRKIMGFGHAVYRTEDPR
                     ATHLRRFSQQMGERIGDMKWYEMSRQVEEVMMREKGLYPNVDFYSASTYYMMGIPLDL
                     YTPIFAVSRISGWTGHVLEQYADNKLIRPRAEYVGVRDARYVPMAQR"
     gene            2130..3224
                     /locus_tag="VFV61_08335"
     CDS             2130..3224
                     /locus_tag="VFV61_08335"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEX4900524.1"
                     /translation="MKRFSTLVLLLALLGCLISTKTAAQTTRSPKPKANVAILIFDGV
                     QIVDYTGPYEVLGSWSRRNVYTVAEKATPITTNMGMRVVPNYSFENNPKPDILVIPGG
                     GNSDPGPKGRGVGAQLNNETLVRWIRESAAQSQYVMSVCNGAFLIARAGLLDGLEATT
                     TAGMTDLLQSVTPKARIVADRRFVDNGKVMTTAGLTSGIDGALHLVAKLDGLGWAQLI
                     AKSIEYNWQPESRYARAALADMHLPDSIYEPIFPVGTPLLMHGGVSAWAEEWSVPLAG
                     DFRARDVLKSINERWAKEKNWIRLNQDSAAESEISVWKFTDRAGKSWIATASAEANEA
                     QPGYALVKLKIASMASLEESAARKGPSELK"
     gene            3275..>3651
                     /locus_tag="VFV61_08340"
     CDS             3275..>3651
                     /locus_tag="VFV61_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900525.1"
                     /translation="MRAKIFVDFLTLALLITVVTVAACSDTGANSDEPASANTDANHY
                     VSFSQSDEDPASAIERYAKTLERNPADANARTALKRLIDCPHPVSNEQSQRIREILRQ
                     YAEVARVSLINSDEPGERLTVSGT"
BASE COUNT          847 a          999 c          992 g          813 t
ORIGIN      
        1 gtccatgtgg cggttgtcga ccccggtgtg ggctcaagca gacgcggaat tgtagttagc
       61 gcggctgaac aaatcttcgt tggaccggac aacggtatct ttagttacat ctacgaacgc
      121 gagacgaatt tgaaggtgta cgagctgagc aatgcaaagt tctttcgcaa acccgtcagc
      181 ccaacttttc acggacgcga tatcttcgcg cccgttgccg cagccctgac gaatggtgtg
      241 aggctcgcga gccttggcaa gcccgttaac gatcctgtca ggctggcatc actgacgccc
      301 gagcgccgag gttcgcggct gctggcgcgc atcattcaca tcgatcggtt tggaaattgt
      361 gtgacgaacc tcacggcaga cctcctcact ccggaaatga tcgaggccgg tgcggtttta
      421 atatcaaagc ggaaaacgat tcgttccttt cgtaaatttt atgaggaaga aactgcggct
      481 cctgaaaagg ttttttgtat ttggggtagt gcgggttttc tcgagatcgc cgccatgaac
      541 acttctgcag cgaaactctt gggcgcggaa cgcggcggta gagtaatagt tcgacgttcc
      601 tgacattctt gcgttccgga ggaacgcgat gtttatagct tgtgcttgcc tttttgtcgt
      661 tgccccggag gggcaaaatg ttgcgctcat gacggagctc aactcaatca tcgagaaccg
      721 tcgctataaa catttggctc cttcggagcc tcgtcgcttt ccattcgctg tttgctgatt
      781 ggctcttccc tttgttatct tgccaacacg ttaaaaattt atgtcagaca aagcaaacgc
      841 acccaccgct ccgtccgctg caggattaag gggagttgcc gccgcgactt cttcgatgtc
      901 agacgtgatt ggcgacaaag gccaactcat ctatcagggt tacgacattc atgacctcgc
      961 tgcgcactca acctttgaag aagtcatttt cctgctctgg aacaagcgcc ttcccaaaca
     1021 gactgagctg gatgaattga agcaagcgct agcaagtgag tatgaagttc cgccgcagat
     1081 aatcgactta atgaagcgca tccctcgcga tgcggatcca atcgatgtgc tcaggacgac
     1141 cgtttcggct cttgagtttt acgatccccg ctcacgcgac atttcgcgtg cggcgagtgt
     1201 cagaactgcc gtcaagttga cggcacagtt tccgactctt gtggccacgg ccgagcgcct
     1261 tcgcaaaggt ttggatcccg tcaaaccgaa tccgcagctc aatatcgcca gcaactttct
     1321 ctacatgctg aacggctccg ctccctctga agcggatgcg cgcatgtttg atatcgcgtt
     1381 aatcctgcac gcggatcatg aacttaatgc ctcaaccttc gcggcgcgcg tcgttgccgg
     1441 aacgttggcg gatatgtatg gcgcgattac tgctgccatt gccgcgctct ccggacccct
     1501 tcacggcggt gcaaacacgg ccgtaatgaa aacgctgctc gagatcggtg aggttgcgaa
     1561 cgtagaaagc ttcatcaaac aggcgctcgc tgataagaga aagatcatgg gctttggcca
     1621 cgccgtctat cgcactgagg atccgcgcgc gactcatctg cgacgcttct cgcaacagat
     1681 gggcgaacgc attggcgaca tgaaatggta cgagatgtcg cgccaggtgg aagaagtgat
     1741 gatgcgcgag aagggccttt atccgaacgt tgatttctat tccgcgtcga cctactacat
     1801 gatgggaatt ccgctcgacc tctacacgcc aatcttcgca gttagtcgta tatccggctg
     1861 gaccggccac gttctcgagc agtatgcgga taataaatta atccgcccgc gggctgagta
     1921 cgtcggcgtc cgtgatgcgc gctacgttcc gatggcccag cgatagaaga ctccgactca
     1981 cttctaccgc taccatttca taccccagaa accccagttc acgtcagcag tctctcacct
     2041 catggcaaat aggtatctgg ctgcgcggga cacacctatc ctgtgctcgc tgcgaaactg
     2101 tgcgcagaac tcaggacgga ggagtgatca tgaagagatt ttcgacactg gtcttactac
     2161 tggcactact cgggtgcctt atctcaacaa agactgcggc gcagacgacg cgctcaccta
     2221 aaccaaaagc caacgtcgcc attttgattt ttgatggagt ccagattgtt gattacaccg
     2281 gtccctatga ggtactaggc tcgtggagtc gccgcaacgt ctacaccgtc gcggaaaagg
     2341 cgacgcccat aacaacgaac atgggcatgc gtgtggttcc caactacagc ttcgaaaaca
     2401 atcccaagcc cgacattctg gtgatcccag gtggtggcaa cagcgatcct ggcccgaagg
     2461 gccggggtgt cggtgcacaa ctcaacaacg agacgttggt gcgctggatt agggaaagcg
     2521 cagcgcaatc gcaatacgtg atgtctgtct gcaacggagc tttcctgatc gctcgcgccg
     2581 gactgctcga tggtttggaa gcaacaacga cggcaggaat gactgacttg cttcaaagcg
     2641 tcacccccaa ggctcgcatc gttgccgacc gccgctttgt cgataacggg aaagtgatga
     2701 cgactgctgg tctgacatcg ggcatcgacg gcgccttgca cctggtggcg aagctcgacg
     2761 gcttgggttg ggcgcagctc atcgccaaga gtatcgaata caactggcaa ccagagtcgc
     2821 gctatgcccg cgccgcgctt gcggatatgc acctgcctga cagtatttac gaaccgatct
     2881 ttcccgtggg cacgccgctc ttgatgcacg gcggcgtaag tgcttgggcc gaggaatgga
     2941 gcgtaccgct cgccggtgac ttccgggcac gagacgtttt gaagagtatt aacgagaggt
     3001 gggcgaagga gaagaactgg atccgactca atcaagactc ggcagctgag tcagagatca
     3061 gtgtctggaa attcactgac cgcgcgggca agagctggat cgctacagct agcgctgaag
     3121 caaacgaagc tcaacccggt tacgccttag ttaagctgaa aatcgcgagt atggcttcgc
     3181 tggaagaatc agcagcgcgg aagggtccct cagaactgaa gtgaccaata tggagcgttg
     3241 tcgttattag gcgaaccatt taatttagaa accaatgcga gcaaaaatct tcgtcgattt
     3301 cctaacgcta gccttattga tcactgtcgt tactgttgct gcgtgctccg acactggcgc
     3361 caactcggat gagcctgcca gcgcaaacac cgatgccaat cactacgtca gtttcagtca
     3421 gtccgacgaa gatcccgcgt ccgcaatcga gcgctacgcg aagacccttg aaaggaatcc
     3481 cgctgatgcg aacgcacgaa cggcactcaa acgcttgatt gattgtcccc atccggtctc
     3541 aaacgaacag tcgcaacgca tcagagaaat tctacggcag tatgcggagg tcgctagagt
     3601 cagtctcata aatagtgacg agccgggtga acgattgacg gtgagcggaa c
//
LOCUS       DASUEX010000498         5203 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1340185, whole genome shotgun sequence.
ACCESSION   DASUEX010000498 DASUEX010000000
VERSION     DASUEX010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5203
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1340185"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(180..2732)
                     /locus_tag="VFV61_08345"
     CDS             complement(180..2732)
                     /locus_tag="VFV61_08345"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX4900526.1"
                     /translation="MTENFSPNTTLAHYRIVSKIGAGGMGEVYLAQDTKLDRKVALKV
                     LPAEVASNRDRMERFVREAKSAAALNHPHIAQIHEIAEDEGTHFIVMEYIEGVSLREK
                     IHSEQTELTKLLRYLQQAAEGLSKAHAAGIVHRDLKPDNIMITRDGFAKILDFGLAKL
                     IEGGGEAVTGRRGEEDPTLAFSPHHPVAPSLSTPGIVMGTVGYMSPEQAQGKTKEIDQ
                     RSDIFSFGCILFEAATAKKAFEGESVIKSLHMVVYEPAPAIADFNPTAPPELQRIVRR
                     CLAKDPDERYQTIKDVAIELKELRRELEGAGINTTVSPTTTSETKAPSGVDTSGSQST
                     RQATSELSASSLSPASSAEYVVAGIKQHKLAAVITLLILVVGLTALGFYLRAPNASGA
                     IESIAVLPFENTSKDADTEYLSDGLAESLIYRLSQLPKLKVTPTSSVMRYRGKHIDVN
                     AIAAELGVSAVMTGRIAQRGDGLTISVELIDARNNQLIWGEQYDRKISDLLATQREIA
                     STVTEKLQLKLSGADAIGITKRYTNNSEAYQLYLKGRYYWNKRTPENIRRAIEQFRAA
                     ADKDPGFALAYVGIADAHMVLLYYTRTSGQEVIPQAKANAMRALEIDDSLAEAHASLG
                     IINNLSWNWAESEKEFKRAIELNPNYATARHWYSRYLRTVGRYNEALTQIRQAHETDP
                     LSLAINDNLAENLMQQGDVNGAIEQCQRSLELDPNYWASHRTLSSAYLKQGRNSEALV
                     EAQKANKLLNNESVSLKTLGYVQAMTGNRKEALAIVKELEARYPKQQADGRDIAEVYA
                     GLGEKDQVFAWLEKDFQRHDHSMAQLRLEAGLDLVRDDPRFKDLMRRMGLPQ"
     gene            complement(2856..4577)
                     /locus_tag="VFV61_08350"
     CDS             complement(2856..4577)
                     /locus_tag="VFV61_08350"
                     /inference="COORDINATES: protein motif:HMM:NF024820.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX4900527.1"
                     /translation="MKRCPQCNRVEVDDTLAFCRTDGVALINDLGSVGGDLATGSGPM
                     SSEIETSLLPHTSTTPEIARNTGPTTALPAEQVQATTRDLTKTKRRGLASASIALVVV
                     VALALGYLYWSRNRTTIIESIAVMPFVNESGNADVEYLADGMTETLINSLSQIPNLSV
                     KARSSVFRYKGKEIDPQKIAAELNVQAILTGRVIQRGDQLTLNLELVDAQTENILWGN
                     RYERKSSELVTLQSEIARDVSNKLKTKLSGADAAKVEKNYTANADAYQLFLKGRFYAV
                     KRTGKDAKQAIDYYQQAVTIDPNYALAYAGLAEANWFLALYSYPQILEAVPKARELAL
                     KAIELDSSLAEPHSILGVICFNYDHDFACMEREAKRAIELNPNYSEGHRRYGLLLVNL
                     GRIQEARLAYKRALEIDPLSPVANLEYARLLFYEKKYEESEVMSKKNVELDPNFWYAH
                     WQLFYVYRMKRDYASAVEELARVQEARGEPDAAKLIRESFVNGDWHGFLRKITEARTR
                     LKLYPYFVATFFAELGEKDRAFALINEAIETKDQHTSQMKIDPFMDPLRDDPRFQEVL
                     KKAGFPE"
     gene            complement(4616..>5203)
                     /locus_tag="VFV61_08355"
     CDS             complement(4616..>5203)
                     /locus_tag="VFV61_08355"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX4900528.1"
                     /translation="SRNILTVLDGAQAVGNIEVDVKAIGCDAYAGPGHKWLMAPKGTG
                     FLYISKAADSRIRPVEWEDGPRYVVGSSGMGSLPLIVGLGAAIEAAQKRGVAACEARN
                     LRLRNRAYEGLKKIAKVQVVSSSPGALATALVAFRLPQAVDSAAFRKAMQQKHNLILK
                     QTEKRWFNGMRISPHVFNTEADIDAALKAIASELS"
BASE COUNT         1115 a         1375 c         1350 g         1363 t
ORIGIN      
        1 agttcgcaat gcaatagctg gaaccgcggc cagcagactc aaactaaagt ttcgacgatt
       61 catttgtgca gttatctaac tcgatcaaac ggcgggagcc gaagaacagc gcgatgctta
      121 aaattgaacc cgaggctgcc gcgtttcggt gctaacggcc gcaatcaccg caggaagaat
      181 cattggggga gccccattcg acgcatcaaa tccttaaatc gggggtcgtc gcggactaga
      241 tcaagacccg cctccaggcg cagttgtgcc atcgagtgat cgtgtctttg aaagtccttt
      301 tccaaccacg caaaaacctg atccttctca ccgagtccgg cataaacctc cgcgatgtca
      361 cgtccatctg cttgctgttt cgggtatcgt gcttcaagtt ccttaacaat tgctagtgcc
      421 tccttacgat tgcccgtcat tgcctgaaca tagcccaaag tcttgagtga aacactctca
      481 ttgttcagta acttgtttgc cttttgagct tccaccaaag cttcggagtt gcgcccttgt
      541 ttcagataag cagatgacag cgtccggtgt gacgcccagt aattaggatc aagttcaaga
      601 ctacgttggc attgttcgat agccccattg acatcgccct gctgcatcaa attctcagca
      661 aggttgtcat ttatggcgag tgacaaaggg tcagtttcgt gagcttgcct aatctgagtc
      721 agagcttcgt tgtagcgtcc aacggtgcgc aggtaacggg aataccaatg acgtgccgtg
      781 gcgtagtttg ggttgagctc aatcgcgcgc ttgaattcct tttccgactc agcccaattc
      841 catgacaggt tattgattat tcccaggctg gcatgggcct ccgccaggga atcgtcgatc
      901 tcaagtgcgc gcattgcgtt ggctttagcc tgcgggatca cttcctgccc ggatgtccgc
      961 gtgtagtaca agaggaccat gtgagcatcg gcgataccaa cgtaggccag cgcaaaccca
     1021 gggtctttgt cggccgctgc tctgaattgc tcgattgccc ttctgatatt ttctggtgtc
     1081 cttttattcc agtaataacg acctttcaaa tagagttggt atgcttcgct attgttggta
     1141 tagcgcttgg tgattcctat ggcgtctgcg cccgatagtt tcaattgtag tttctcggta
     1201 actgtcgaag cgatctcccg ttgagtcgcc aacaggtccg aaatcttgcg gtcatactgc
     1261 tctccccaaa tcagctggtt attgcgggca tcaatcagtt cgacgctaat cgtaagacca
     1321 tcgccgcgtt gggctatgcg cccggtcatt actgcactga cgcccagttc agcagcgatt
     1381 gcattgacgt ctatgtgctt gcctctgtac cgcatcacag aactggtggg ggtgactttc
     1441 aattttggta gttgagatag gcgataaatc agcgactcag ccaaaccgtc ggagagatac
     1501 tccgtatcag catctttgct tgtgttctca aagggaagaa cggcgatgga ttcaatcgct
     1561 cccgaggcat tgggagcacg cagatagaaa ccgagagctg taaggcccac cacaagtatc
     1621 agtagcgtga taacggccgc gagtttgtgc tgtttaattc ccgcaacaac gtattcggcg
     1681 ctagaggcgg gactcaagct tgaagcagac agctcactgg ttgcttgccg cgtgctttga
     1741 ctgccgctgg tatcaactcc ggagggtgcc tttgtttcgc tcgtggtggt tggcgagaca
     1801 gttgtgttga taccagctcc ttcgagttcg cggcgtagct ccttcaattc tatcgctaca
     1861 tctttgatcg tttgatagcg ttcgtccggg tccttcgcga gacaacgccg cacaatgcgt
     1921 tgcagctctg gcggcgcagt tggattgaag tcggcgatgg ccggtgcagg ctcgtacaca
     1981 accatgtgca gcgacttgat gactgactca ccttcgaagg ccttcttcgc ggtcgccgct
     2041 tcgaagagaa tgcagccgaa tgagaagatg tctgacctct ggtcgatctc tttggttttg
     2101 ccttgtgcct gttcgggcga catgtagccg acggtgccca tcacgatgcc gggcgtagaa
     2161 agggacgggg cgacagggtg atggggagag aaggcgagtg tcggatcttc ttcgccccgt
     2221 ctccccgtca ccgcttctcc ccctccttcg atcaattttg cgagaccaaa atccagaatc
     2281 ttcgcaaatc catctcgcgt gatcatgatg ttgtcaggct tcagatcacg gtggacgatg
     2341 ccggcggcgt gtgctttcga taatccttcg gccgcttgct gcaaatatct caacagcttt
     2401 gtgagttcgg tttgctcaga atgaatcttc tcgcgcaagc tcacgccttc gatgtactcc
     2461 atcacgatga agtgtgtgcc ttcgtcttcg gcaatctcat ggatctgggc aatgtgtgga
     2521 tggttgagcg ccgcggcaga tttagcctcg cgcacgaacc gctccatgcg atcgcgattc
     2581 gaagcaacct cagcaggcaa gactttcagc gcgactttgc gatcgagttt cgtgtcctga
     2641 gccaaataca cttcgcccat cccgccggca ccgattttgg agacgatgcg gtaatgggcg
     2701 agcgttgtgt tgggggaaaa attttcagtc actgggttat gagttgctta agcgaaagtg
     2761 tgctgcgagc gaatgtcttc atcaaggtta tcaattcgag gacaggtcag cgctgcgacg
     2821 cgattgcctt gggggcatcg tttcatgaat tgattctatt caggaaaacc ggctttcttc
     2881 agcacttcct gaaaccgcgg gtcgtcacgc aatggatcca tgaatgggtc tattttcatc
     2941 tgactcgtat gttgatcttt tgtttcgatc gcttcgttga tcaaggcaaa ggctctatcc
     3001 ttctcgccca actctgcaaa gaaagttgcc acaaaatagg gatacagctt gagccgcgtt
     3061 cgggcctcgg tgatcttcct cagaaagcca tgccaatcgc cattcacaaa actctcgcga
     3121 atcaactttg cagcatcggg ttcgcctcgc gcctcctgga ctctggcaag ttcttcgacg
     3181 gccgaagcgt agtcccgctt catgcggtaa acataaaaga gttgccaatg cgcataccag
     3241 aaatttggat cgagttcgac gttcttcttg ctcatcacct cgctttcttc atactttttt
     3301 tcatagaaaa gcaaacgcgc atattccagg ttagcaaccg gcgatagcgg atcgatctcc
     3361 aacgctctct tataagcgag cctggcttct tggattctgc ccaagtttac aagcagcagt
     3421 ccgtaccgac gatgaccttc cgaataattt ggatttagct cgatcgcacg ctttgcctcg
     3481 cgctccatgc atgcgaaatc gtgatcgtag ttaaaacaga tgacccccaa tatcgagtgc
     3541 ggctccgcta agctgctgtc caattctatg gctttcagtg caagctcccg tgccttgggt
     3601 accgcttcta gtatttgcgg ataactgtaa agggcaagga accaatttgc ctcggccaga
     3661 ccggcatatg ccaaagcgta gttcggatca atggtaactg cctgttggta ataatcgatt
     3721 gcttgttttg cgtctttccc ggtgcgctta actgcgtaga accgcccttt caaaaacagt
     3781 tgataggcgt ccgcgttggc ggtgtaattc ttttcgactt ttgctgcgtc agcacctgac
     3841 agtttggttt taagtttgtt tgaaacatcg cgggcaatct cgctttgaag ggtaacgagt
     3901 tctgaggatt tgcgttcata tctgttgccc cacaaaatgt tttccgtctg tgcgtcaacc
     3961 aattccaaat tgagggttag ttgatcgcca cgttggatga cccgccccgt gagtattgcc
     4021 tgcacattca gttcagcagc aatcttttga gggtcaatct cttttccttt gtaacgaaaa
     4081 acggatgagc gtgcctttac gctcaagttt ggaatctgtg aaagactatt gatgagcgtt
     4141 tcggtcattc cgtccgcgag gtactcgaca tccgcattac cgctctcatt gacgaagggc
     4201 atcacggcga ttgattcaat tatggtcgtc cgatttcgcg accagtacag gtagccgaga
     4261 gcgagagcaa caacaacaac caacgcgata ctggcggaag ctaatccgcg tcgcttcgtt
     4321 ttggttaagt cgcgtgtggt tgcttgaacc tgctctgcgg gaagtgccgt tgttggtcca
     4381 gtgttgcgag ctatttcagg agttgttgag gtgtggggaa gcagactggt ttcgatctca
     4441 ctcgacatcg gaccagagcc ggtggccagg tcaccgccga ccgaaccaag atcgttaata
     4501 agcgcgactc catccgtgcg gcagaaagcg agcgtgtcgt cgacttcaac gcgattacat
     4561 tgggggcagc gtttcataga tcgcagtcta cgccgagcgg tgtatgcccg agacctcagg
     4621 agagttctga tgcaatcgcc ttgagtgcgg catcaatgtc agcctccgta ttgaacacgt
     4681 gtggcgaaat ccgcattccg ttgaaccagc gtttctcagt ctgcttcaga atgagattgt
     4741 gtttctgctg catcgcctta cggaatgctg cactgtcgac tgcttgcggc agcctgaacg
     4801 cgactaaggc agtcgcgagc gctccaggcg aggagcttac tacctgcacc ttggcgatct
     4861 ttttcaagcc ctcgtatgcg cggttcctga gtcgaaggtt tcgtgcttcg cacgcagcga
     4921 ctccacgttt ttgcgctgcc tcaatagcag ctccgagccc gacgatcaaa ggtaaggatc
     4981 ccatgcctga cgatccaact acatatctgg ggccatcctc ccattcaacc ggtcgtatcc
     5041 gcgaatctgc ggctttgctg atatacagaa accccgtacc cttcggcgcc atcagccact
     5101 tatgtccagg gccagcgtag gcatcgcaac cgatcgcttt tacgtcgacc tcaatatttc
     5161 caacggcctg ggcgccgtcg aggactgtca ggatgttccg gct
//
LOCUS       DASUEX010000499         3397 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1006029, whole genome shotgun sequence.
ACCESSION   DASUEX010000499 DASUEX010000000
VERSION     DASUEX010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3397
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1006029"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..875
                     /locus_tag="VFV61_08360"
     CDS             <1..875
                     /locus_tag="VFV61_08360"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEX4900529.1"
                     /translation="PLTISGSDSLRAITYDLPVSSAQVKSCVLLAALNAVGMTKVSES
                     LVKSRDHTERMLQWFGASLSQQTVGGTHVVSVAGPGTLSARDIMIPGDLSSAAYFVAA
                     AGLLPNSDLIIDNVGLNPTRTGVLALFESLGLELEVKNVREASNEPLGSIRVSGRQQI
                     LGSETALSGATSAQVIDELPLLAVVGTQVPGGIEIRDAEELRLKESDRIATTVENLRA
                     MGATVKEFDDGMRVSSVQLKGARVNTHGDHRIAMAFTIAALMAEGESEIEDAECVCVS
                     FPEFFEVLESIVEW"
     gene            995..1510
                     /locus_tag="VFV61_08365"
     CDS             995..1510
                     /locus_tag="VFV61_08365"
                     /inference="COORDINATES: protein motif:HMM:NF013375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEX4900530.1"
                     /translation="MNKIIITGFMGSGKSSVARALADILGYEMLDLDEAIAVAEGRSI
                     AAIIQDEGEPRFRELEQRALAEALEGPGKQILALGGGTWIPETNRKHIREHGATSVWL
                     DAPFNLCWKRIAVAAGRRPLAPDETSAFKLFSARSSCYELADLRVAVTEQRSAGEIAA
                     EIAHALTKRSN"
     gene            1512..2489
                     /locus_tag="VFV61_08370"
     CDS             1512..2489
                     /locus_tag="VFV61_08370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900531.1"
                     /translation="MSNLTQTDHDHRGIISALVADGRPLLIFTGLALILAGAFALFLS
                     ANQHFLPHDVQFLGMTAEQLCGIQSCRIVYFMFHDRVAFGGALISVGALYMWLAEFPL
                     RERKAWAWWVFIISGLSGFGSFLAYLGYGYLDTWHGVATMFLFPLFIVGLARSRQFLE
                     TPRGIRSLCQSGVSAGLRSRYGFGRALLLATAAGMIAGGLTIMTVGMTWVFVPQDLEF
                     MGVQTIELQSVSSRLIPLIAHDRAGFGGAICTTGIAVLFCVWCARPSKSLWQVLCFSG
                     VVGFAAAIGIHPVVGYTNLLHLAPAILGAVIFIIGLVLTYPTMHRAFAD"
     gene            complement(2586..>3397)
                     /gene="ligD"
                     /locus_tag="VFV61_08375"
     CDS             complement(2586..>3397)
                     /gene="ligD"
                     /locus_tag="VFV61_08375"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEX4900532.1"
                     /translation="EKFTKFDLLRYYIQIAPHLLPHLKDRPAILQRWPRGINAPMFFQ
                     QDLESAPSFVKSVHLTNLEGRELDYAVFSTTAALLHLVNLGNIEQHPWHSTTKKLSHP
                     DWIALDLDPKKAPWQNVLEVALLVKQVCDEVGLPAFPKTSGSSGIHIYLPLKPTNEYN
                     AVAEYSRLLASEVASRAPKIATVERSLAKRGKDQVYVDWMQNARGKSLASVFTVRAKP
                     GATVSMPLTWKQVEKGVKISDFTIKNVPALIQKKGNAWEDFFAQRQKLKLN"
BASE COUNT          695 a          894 c          968 g          840 t
ORIGIN      
        1 cgccgttgac tataagcgga tctgattcgc taagagcgat tacttacgat ttgccggtaa
       61 gcagtgccca ggtaaagtcg tgcgtcttgc ttgccgcact caatgcggtg gggatgacta
      121 aggtgagcga atctctggtt aagtctcgcg atcatactga gcgcatgttg cagtggtttg
      181 gagcttcact gtcgcagcag actgttggcg ggacgcatgt cgtcagtgtt gctggcccgg
      241 gaacgctttc ggcgcgggac ataatgatcc ccggcgacct ctcttccgcc gcttactttg
      301 ttgccgccgc cggactgctc cctaactccg atttaataat agacaacgtc ggcctgaatc
      361 cgactcgaac cggagtcctg gctctgttcg agtctctagg tctggaactt gaggtcaaaa
      421 atgtgcgcga ggcaagtaat gaaccgctcg gcagcatccg cgttagcggc agacagcaga
      481 tattgggatc ggaaacagct ctctcaggag caaccagcgc gcaagtgatc gacgaactac
      541 cgctcttggc agttgtggga actcaggttc ccggagggat tgaaatccgc gacgccgaag
      601 aactgcgatt aaaagagagc gataggattg cgacaacagt agagaacctg cgcgcgatgg
      661 gtgccacggt gaaagagttt gatgacggca tgagagtcag ttccgtgcaa ctcaaaggag
      721 cgcgcgtcaa tacccatggc gatcaccgca ttgcgatggc gttcaccatc gccgccctta
      781 tggccgaagg tgagtcggag atcgaagacg ccgaatgtgt gtgtgtttcg tttccggagt
      841 tttttgaagt cctggaatcg attgtggagt ggtaacaagt cttgaaccct gagagggaat
      901 tagccacgga tttacgcgga tttcacagat cgacttcttt ctgaatctga gtttatcggc
      961 gtgaatctgt ggccagtcct aacattcttt agtcatgaat aagatcatca tcacgggctt
     1021 tatggggtcg ggtaagagtt ccgtcgccag agcattagcg gacatcctgg ggtacgagat
     1081 gcttgatctg gatgaggcaa ttgccgtcgc ggaaggtcgc tcgatcgccg cgatcattca
     1141 agatgaggga gaaccgcgat ttcgggaact ggaacagcgc gccctggctg aggctctgga
     1201 agggcccggc aaacagatcc tcgcgttggg gggcggcacc tggatcccag aaacgaatcg
     1261 caaacatatc cgcgaacacg gagcaaccag cgtttggtta gatgcgccct tcaatctctg
     1321 ttggaaacga attgcagtcg cggccggtcg aagaccttta gctcccgacg aaacctccgc
     1381 ctttaagctt ttctccgcgc gctcctcatg ttatgaactc gcggacctgc gcgttgcggt
     1441 gaccgagcaa cggtcggctg gcgaaatcgc ggcggagatt gcgcacgcgc tgacgaagcg
     1501 ctccaattga tttgagcaac ctgactcaga ctgatcacga tcaccgcggc atcatctccg
     1561 cactcgtagc cgatggtcgc ccgttgttga tcttcaccgg cctggctctg attctggccg
     1621 gggctttcgc gctcttcctt tccgccaatc aacactttct gccgcatgac gtgcaatttc
     1681 tgggcatgac ggccgaacag ctttgcggta ttcagagttg tcgcatcgtt tactttatgt
     1741 tccatgatcg cgtcgccttt ggaggcgcgc tgatttcagt gggcgccctt tacatgtggt
     1801 tggcggaatt tcctctgcgc gagcgcaaag cctgggcctg gtgggttttc attatcagcg
     1861 gcctgtcagg gtttggaagc tttctggctt atctcggcta tggttatctg gatacctggc
     1921 acggcgtggc aacgatgttt ctctttcctt tgttcatcgt cggtctggcc cgttcaagac
     1981 aattcctgga aactccacgc ggcattcgtt ccttgtgcca atccggtgtt agcgcaggcc
     2041 ttcgcagtcg ttatggcttc ggtcgtgcgc tcctactcgc gacggcggcc gggatgattg
     2101 ccggcggcct gacgatcatg acggtgggaa tgacctgggt ctttgtacct caggatctcg
     2161 agttcatggg agtgcaaacc attgagctac agtcagtcag tagccggctc ataccactta
     2221 tcgcccatga ccgagcgggc tttggcggcg caatctgcac taccggcatt gccgtgctct
     2281 tttgtgtttg gtgtgcgcgg ccttcgaaga gtctctggca ggtgctctgc ttctcgggag
     2341 tagtcgggtt tgcagctgcg atcgggattc atccagtggt gggctacacc aatctcctgc
     2401 accttgctcc agcgattctt ggagctgtca tctttattat cgggctcgtc ctcacctatc
     2461 cgacaatgca tcgagcgttt gcggattgat gcgttggatt gccgattagc ccgcagcgga
     2521 atagggtcat accttcaggc cgtagccgct gatgctacac taatcgggaa ttgccaattg
     2581 gcaatttagt tcagtttcag cttttgacgt tgcgcgaaaa aatcttccca ggcgtttccc
     2641 tttttctgaa tcagtgccgg tacattctta atggtgaagt ccgaaatctt tacgcccttt
     2701 tccacctgct tccaggttaa gggcatggaa acggttgctc ccggttttgc gcgtaccgtg
     2761 aaaacagaag ccaggctctt gccacgcgca ttctgcatcc aatccacata gacctgatcc
     2821 ttccctcgct ttgcaagcga gcgttcgacg gtggcaatct tcggcgcgcg gctcgccact
     2881 tcagatgcga gcagacggga gtactccgca accgcgttgt actcgttcgt cggtttcagt
     2941 ggtagataaa tatgaatgcc ggatgaaccc gaggtcttgg ggaaagcagg caatcccact
     3001 tcatcgcaaa cctgcttcac caacagggca acctcgagga cgttttgcca cggtgctttc
     3061 ttcggatcga gatccaaagc gatccagtct gggtgcgaaa gctttttggt ggtcgagtgc
     3121 cagggatgct gttcgatgtt tcccagattg acgagatgga ggagtgccgc cgtcgttgag
     3181 aagactgcgt aatcaagttc gcggccttcc aggtttgtca gatgaactga ctttacaaaa
     3241 ctcggcgcgc tctccaggtc ctgctgaaaa aacatgggag cgttaatgcc gcgcggccaa
     3301 cgctgcaaaa tcgccggacg gtctttcaaa tgcggcaaca agtgcggtgc aatctgaatg
     3361 tagtagcgca gcaggtcaaa cttggtgaac ttctcaa
//
LOCUS       DASUEX010000500         3626 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_651158, whole genome shotgun sequence.
ACCESSION   DASUEX010000500 DASUEX010000000
VERSION     DASUEX010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3626
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_651158"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="VFV61_08380"
     CDS             <1..389
                     /locus_tag="VFV61_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900533.1"
                     /translation="LSSAKAQAIANPDMVAKIPFEFSVGDKTLPAGEYPVRCINPNSS
                     TKVLQIRSKTGSARALVMTDSVSGKIKENALLIFFRYGNEYFFTQAWMPANSVGLQAP
                     RSKALKALELAREKRETETVVASMRP"
     gene            619..1911
                     /locus_tag="VFV61_08385"
     CDS             619..1911
                     /locus_tag="VFV61_08385"
                     /inference="COORDINATES: protein motif:HMM:NF013056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEX4900534.1"
                     /translation="MSSSQHASPSIRSEALAGITTFFTMSYIVIVNPSILSTEGTGMA
                     FSGVLTATVLLCFVMTLFMGLYAKLPFAVAPGMGINAFFTFTIILTQKVWWQVALGII
                     FWAGVLFLLISITPIRETIAKAIPAELRVGTAAGIGVFLTFIGLKNAGLIAPDPVTFV
                     KLGSLGVPALLTILATGLSVWLVTRKSALAFLAGILFATLLGWVFGLVKAPPSFFSAP
                     DFQTVFLKLDILGALKLSLAPAIIAILFTDLFDSISTFIGVAHAADLIDEDGHPRNLK
                     QGLIVDSFATLGAGLAGTSSGTAYIESVAGINMGGRTGLTSVFTALCFLPCFFLAPIA
                     GMVPAYATASVLILVGASMFKSVSQISFSKIEEGLPAFLTIILIPLTFSITQGILWGF
                     ISHVGMYLLVGRRREIHPVMYVLALISIGLLLLEHVRF"
     gene            2025..>3626
                     /locus_tag="VFV61_08390"
     CDS             2025..>3626
                     /locus_tag="VFV61_08390"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900535.1"
                     /translation="MNAISWALLPLRRVMLGVTIVALATAVSAQDPTPTPADQRGIGV
                     QSKTANQTSAGNQQGKEAKPELVLQTGYNNLFGATRLVFSPDGRLLATGTFRSSTIKL
                     WDTSNGRELRNLSGGRQSGMSLAPYIAFSNDSRLVAAAAGGTAVKVWEVASGREVQTL
                     DSGSEGMVSAIAGVSFIAFNPSGQIVTISDAIRVWDLATGQALRTIGSEASDALASLG
                     GNGGAAISLDGTQLATAATAAENEIKFFDLATGRETRTVKLPDNELETIDLTFAPDGR
                     LLVAALMDKRLKVWDVTNKASERDLGSAPAEWSLLRFSRDGRFLALSEGYAVRLWEVA
                     SARELPALKAPSSGFVVTTNRIYASFSEDGKRIATGGFDTPTIVWEADSGKQLLKMNG
                     RTNMAYGVAFSNDGNRLSSGGRTTWDLRTGRGLRLAAAPIDQQLSIPSPDGKLLAVFS
                     PTGNAVTIVETPSGRKLQSLTPAGQSVVQRASFSADSNLLAVTYTASEAQMREGKLPG
                     QAGSNESPVRIWDVKSGRELRALNLG"
BASE COUNT          805 a          982 c          981 g          858 t
ORIGIN      
        1 gcctttcatc tgcaaaggca caagccatcg cgaatccaga catggtcgcc aaaatcccgt
       61 tcgagttcag cgtcggcgac aagaccttgc cggctgggga gtatccagtg cggtgcatca
      121 atcccaattc gtcgaccaaa gttttgcaaa tccggagcaa gacaggttca gcccgtgcgt
      181 tggtgatgac tgacagcgta agcggcaaga taaaggaaaa tgcgctgctg atcttcttcc
      241 ggtatggcaa cgaatacttc tttacccagg catggatgcc agcgaacagc gttggattgc
      301 aggcgccacg gtcaaaagct ctgaaggctc tagagctagc ccgcgagaaa cgagagacgg
      361 aaactgtggt ggccagtatg cgaccttagc gcacaagcct ttgaaaagac tcagcaagca
      421 gagaatttgg gaactcgctg cgagctgaca accgacaacc cggagggcaa ccttcgggtt
      481 gttgtttttc attatgaact aactgcgctg ctgcttttct gcaccgcgaa agccgttgct
      541 aatggtgttg cgcggtactg gtccctgtct tagacttcca tctgttacgc ccatctcctt
      601 ccagacatct ctctgcaaat gagctcttcc caacatgcca gtccaagcat tcgctccgag
      661 gcactcgccg ggattaccac cttcttcacg atgtcttaca tcgtgatcgt taacccttca
      721 attctttcaa ccgaaggtac cgggatggcg ttcagtggag tgcttacggc gacagtcctg
      781 ctctgttttg tgatgacgct cttcatgggc ctttacgcga aactcccatt cgctgtcgct
      841 cccgggatgg gcatcaatgc cttcttcact tttacgatca tcctgacaca gaaagtctgg
      901 tggcaggtcg cgctcggaat catcttctgg gccggggtat tgttcctgtt aatttccatt
      961 actccgattc gcgaaaccat cgcgaaagca attcctgcgg agctgcgcgt cggcactgcc
     1021 gccggcatcg gtgtcttcct cacattcata ggcttaaaga acgctggatt gattgctccc
     1081 gatccggtca catttgtgaa gctcggctca ctaggcgtac cggcgttgct gacaatcctg
     1141 gccacaggtt tgtcagtctg gctggtcaca cgcaaaagcg ctttggcttt tctggcgggt
     1201 attctgtttg caaccctgct cggctgggtc tttggtctgg taaaagcgcc gccaagtttt
     1261 ttcagtgccc ctgacttcca gactgtgttt ctcaaactcg atatccttgg tgcgctgaag
     1321 ctttcgctgg cgccggcaat tatcgcgatc ctcttcactg atctttttga ttccatttct
     1381 acgtttatcg gcgtggctca cgcggcggat ctaattgacg aagatggcca cccgcgcaat
     1441 ttgaaacagg gactgatagt tgattctttt gccaccctcg gcgccggcct ggcaggcaca
     1501 tcttcgggaa cggcttacat cgaaagcgtc gccggaatta acatgggcgg gcgcacgggt
     1561 ctcacctctg tttttaccgc gctctgtttt ctcccctgct tctttctggc gccaatcgca
     1621 ggaatggttc cggcgtatgc gacagcatcg gtgttgattc tggtcggggc ctcgatgttc
     1681 aagagcgtca gtcaaatctc gttttccaag atcgaagaag gactgcctgc atttctgaca
     1741 atcattttga ttccacttac gttctctatt acacagggga ttctgtgggg cttcatttca
     1801 catgtgggga tgtatcttct cgtcggtcgg cggcgggaga ttcatccggt aatgtacgtg
     1861 ctggcgctga tctcgattgg attgctattg ttggagcacg tgcggttcta atattgaaag
     1921 attgaatcat cggatcattg aaagattgat ccactgaggt cacgagtatt atccaatgaa
     1981 ccatgaacca atgattcaat gactcaattg taacgggggt ttttatgaac gcaattagct
     2041 gggcattgtt gccgctcaga agagtaatgt tgggagttac gatcgttgct ttagctactg
     2101 cggtctcggc tcaagatccc actcccacgc ctgccgatca acggggcatt ggcgttcaga
     2161 gcaaaaccgc taaccaaacc tccgccggca accagcaggg aaaagaagct aagcccgagc
     2221 tcgtactcca aactggatat aacaatttgt tcggcgccac ccgcctcgtt ttcagtccgg
     2281 acggaaggct gctggcaaca ggcacatttc gcagcagcac gatcaaactt tgggacacca
     2341 gcaacggacg cgagctgcgc aatctttccg gcggccgcca aagcggaatg agtctcgctc
     2401 cctacatcgc cttcagcaac gacagtcgtc tggtggctgc agcagcgggc ggaaccgcgg
     2461 tcaaagtctg ggaagtggcc agcggtcgcg aagtccaaac actcgactcc ggatctgaag
     2521 gtatggtctc tgctattgcg ggagtctcct tcattgcgtt caacccgagc gggcaaattg
     2581 tgacgatcag cgatgccatc cgagtttggg atctcgccac tggacaggca ttgcgtacga
     2641 ttggcagcga ggcctcggat gcgttagcca gccttggcgg caacggtgga gcagcaataa
     2701 gtttggacgg cacacaactg gctactgcgg caactgctgc tgaaaatgaa atcaagttct
     2761 ttgacctcgc aaccggtcga gagactcgca cagtaaaact tccagacaat gagctcgaga
     2821 ccattgatct gacatttgct cccgacggcc gcttactcgt cgctgctttg atggacaagc
     2881 gcctgaaggt gtgggacgtg acaaataagg caagcgagcg cgacttggga agtgctcccg
     2941 ccgagtggag cctcttgaga ttcagtcgag atggtcgttt cctcgcacta tcggaaggat
     3001 atgcggtcag actttgggaa gtcgccagcg cgcgggagtt gccggctctt aaggcgccca
     3061 gcagtggctt cgtagtaacc acgaatcgca tttacgcaag tttcagcgaa gacggaaaga
     3121 gaatcgctac cggagggttc gatacaccaa ccatcgtttg ggaggccgac agcggaaagc
     3181 agttattgaa gatgaacggc cgcaccaaca tggcctacgg tgttgcgttc agcaacgatg
     3241 gcaatcgcct ctcttcagga ggccgcacta cctgggacct gagaacaggt cgaggattac
     3301 gtctcgctgc cgcgccaatc gatcagcagt taagcattcc cagccccgac ggaaagttgc
     3361 tcgctgtgtt ttccccaacc ggtaacgcgg taacgatagt ggaaacgcct tctggtcgaa
     3421 aacttcaaag cctcactccc gcaggccaaa gcgttgtaca gcgcgccagc ttcagcgctg
     3481 acagcaatct gctcgcggtt acttacacgg ccagtgaagc gcagatgcgg gaaggaaagc
     3541 ttcccggcca ggcagggagc aacgagagtc ccgtcagaat ctgggacgtc aaatccgggc
     3601 gtgaactgcg cgcacttaat ttggga
//
LOCUS       DASUEX010000501         3561 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_651325, whole genome shotgun sequence.
ACCESSION   DASUEX010000501 DASUEX010000000
VERSION     DASUEX010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3561
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_651325"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..753
                     /locus_tag="VFV61_08395"
     CDS             <1..753
                     /locus_tag="VFV61_08395"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX4900536.1"
                     /translation="AFQVRVLTHGTNYMSQTRRVEVVRPLGFGAISEQVEFVLTSRET
                     TTTATRGVLFVQEVPAPARKEFERGAALIKKPEQQKAALASLNKALEIFPQYYDALEL
                     LGTEYVKQQQYEPAVPLLTKAVEINPRGQASWYALGVAQYNLKQSPAALEALRRSVSL
                     SPRSINSHLWLGIVLRLSGKLDEAETSLRAADKLADSKLPDPHWQLALLLNQMKRYKE
                     AADQLELFLKVEPNARDTELIKKLIKRLREQG"
     gene            complement(871..1986)
                     /locus_tag="VFV61_08400"
     CDS             complement(871..1986)
                     /locus_tag="VFV61_08400"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX4900537.1"
                     /translation="MPLDRRMLFLIHTSLLLVLLLSPNTVFADSVNSKERTVTKLAEN
                     IYVIRHKDAPDGFPQGNTTVIIGDLEVLVVDSCYLPSSAREDIAQIRQWTNKPVRYLV
                     NTHWHYDHTMGNGTYAEAFPQLGIIAHTETRKQMEGYNPRWFEKYPQRATTFKQRLDS
                     GKNLDGSPLTEIQRKDFATALEGVAPVSAEFKTIVDRLPTLTFDRELQVDVGNRVVQI
                     KHLGRGNTAGDTIVYLPAEKILIAGDLLDYPVPYLGGGYPSELIKTLERLGELEEQTI
                     VPGHGEVLSGGRGEAHRRLVIEFLQTIVSQVSREIYRIGNGPRNLEPVREAVLKTIDL
                     ASWRERFGGGDKDNVDFFNTFSLPGVITAAYAELWGR"
     gene            complement(2124..>3561)
                     /locus_tag="VFV61_08405"
     CDS             complement(2124..>3561)
                     /locus_tag="VFV61_08405"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX4900538.1"
                     /translation="GSTNTAIYAQDSWQPINRLTLNLGVRFENEDVPSFSPNGQAIKF
                     GWGDKIAPRLGFAYDVFGDGRMKVFASYGQFFDRFKYQLPRGSFGGDKLLRYFFPLAD
                     PNWTTYTRAYTLAHTLVGPLDFRVPSNDPSDNRVDPDLKAARQTEFTVGTEYGLGRDI
                     VLSGRYTRKNLDRTIEDVGFIDGDGNENFFIANPGLGVVSQPFAPGLVASPKAERQYN
                     AMELRLDKRFANNYFISASYTYSRLTGNYSGLSSSDEPDALGVGRSSPNVNRFFDLPF
                     LGFNTNGQPDNGLLATDRPHALKLFGAYTIDWKNLFGKVIDSSGSNSTELSASFVGMS
                     GTPLSTRVQLYGAYTFLTTRGDLGRTPMFTQTDLHMTHTIKMGNDGRFALKLDLNLLN
                     AFNQNNVLSVWTQVSPDSLTANTFGIAGATAGIRELNTFRAVFNGGLTNQILAGLANG
                     TISRDARFSQPLTRQIPREIRFGFRFVF"
BASE COUNT          854 a          907 c          969 g          831 t
ORIGIN      
        1 gcctttcagg tccgagtatt aactcacgga acgaactaca tgagtcaaac caggcgcgtg
       61 gaggtggtta gaccgcttgg ttttggcgcc atctcagaac aggtggaatt tgttttgacc
      121 tcacgagaga caaccacgac ggcgacacgc ggcgttcttt tcgtgcaaga ggttccagcg
      181 ccggcccgca aggagtttga gagaggcgct gcgcttatta aaaagccaga gcaacaaaag
      241 gcggccctgg cctcactgaa caaggcgctc gaaatttttc cgcaatacta cgacgcactt
      301 gaattgcttg ggactgagta tgtaaagcaa cagcagtacg agcccgcagt gccgctcctg
      361 acgaaggcgg tggaaataaa cccgcgtgga caagcgagtt ggtacgcact cggagtggcc
      421 caatataatt tgaagcaatc accggctgcg ctcgaagccc tgcgacgttc ggtttctctt
      481 agcccaagat ccatcaactc tcatttatgg cttgggatcg ttttgagatt gagcggcaag
      541 ctggatgaag ccgagacttc tctcagggcc gcggacaaat tggcggactc taagttgcca
      601 gatccacact ggcaattagc gctcctgctt aaccagatga agagatacaa ggaagccgcc
      661 gatcagttgg agctcttcct caaggtcgaa ccaaatgcga gagataccga actgatcaag
      721 aaactgatca aacggttgcg agagcaggga tgaagacgga aggatgaagg ataaaggcgg
      781 gttttcgacg ctgtttggtt cacttaaggg aaagttatag gtatcggggt caagtgtttc
      841 atccttcatc cttcagcctt catcctttct tcatcttccc caaagttccg cgtacgccgc
      901 agtgattacg cctggtaggc taaaagtgtt gaagaaatcc acattgtctt tatcaccacc
      961 cccgaagcgc tctcgccacg atgccaggtc gatagttttc aacaccgctt cacgcacagg
     1021 ctcaagattg cgtggtccat ttccaatccg atagatctca cggctgacct gagagactat
     1081 ggtctgcagg aattcaataa ccaggcgccg gtgcgcctca ccgcggccgc ctgaaaggac
     1141 ctcgccatga ccgggaacaa tggtctgctc ctccaattcg cccagcctct ctaacgtttt
     1201 gatcaactct gaaggatagc caccgccgag atatggaacg ggataatcca acagatcacc
     1261 ggcgatcaga atcttctcgg cgggcaggta aacaattgtg tcaccggcgg tgtttccacg
     1321 accgaggtgc ttgatctgta ctacccggtt gccaacatcg acctgcaact cgcgatcaaa
     1381 tgtcagggtg gggagacgat ccacgatggt cttgaattca gcagagaccg gtgccacccc
     1441 ttccagagcg gtcgcgaaat ctttccgttg gatctccgtt aatggtgaac catccaggtt
     1501 tttacctgaa tcgagtcgtt gcttgaaggt agtcgcccgc tgcggatact tttcgaacca
     1561 gcgagggtta tagccttcca tctgtttgcg ggtttcagtg tgagcgatga ttccgagttg
     1621 ggggaacgct tcagcatagg taccgtttcc cattgtgtga tcgtaatgcc agtgggtgtt
     1681 caccagatag cggacaggtt tgtttgtcca ctggcggatt tgcgcgatgt cctctcgcgc
     1741 gctggatggc aggtaacagg aatcgacaac cagcacttca aggtcgccga tgatcacggt
     1801 tgtgttgccc tgaggaaaac cgtccggggc gtccttgtgt cggatcacat agatattttc
     1861 cgcaagcttc gtaacggttc gttctttgct gttcaccgaa tcggcgaata cggtattggg
     1921 agagagaagc agcacaagca gcaagctggt atgaattaag aagagcatcc ttctgtctaa
     1981 tggcattttg atttttctcc tgtcaatgtc tggcaattcg ttaggccggc tccagcaaat
     2041 gcgagcgtac ttggcgttgg taataacaaa gcccacccag atttcctgga tgggctttgc
     2101 tgtttgttga ttcgcaccgt cggttagaaa acaaaccgga agccgaacct aatctcacgc
     2161 ggtatttgcc gtgtgagcgg ctgactgaaa cgggcgtctc tggagatagt cccgttagcc
     2221 aaaccagcca ggatctggtt ggtgagccct ccgttgaata ctgcacggaa ggtgttgagt
     2281 tcccgaatcc cagccgtcgc gccggcaata ccaaaggtgt tggcggtcaa ggaatccgga
     2341 gagacttgcg tccaaacact caggacgttg ttttggttaa aggcgttcaa gaggttgagg
     2401 tccagcttca gtgcgaagcg gccgtcattc cccatcttga ttgtgtgcgt catgtggaga
     2461 tctgtctgcg taaacatcgg agtgcggccg agatctccgc gagtcgtcaa gaatgtataa
     2521 gcaccataga gttgcacgcg cgtcgataac ggcgtaccgc tcatgccaac aaacgaagct
     2581 gaaagctcag tgctgttact acccgagcta tcaatcacct taccaaacaa gttcttccaa
     2641 tcgatggtat atgcaccgaa tagcttgagt gcatgcggtc gatcggtggc cagcagtccg
     2701 ttgtccggct gtccattggt gttaaagccc aggaacggca gatcgaagaa tcgattgacg
     2761 tttggacttg aacgacccac gccgagagca tcaggttcat cggaactcga gagacccgag
     2821 taattacctg tcaaccggct gtacgtgtag ctggcactga tgaagtaatt atttgcgaac
     2881 cgtttgtcga gacgcagttc catcgcgttg tactgacgtt cagcctttgg cgatgcaacc
     2941 aggcctggag caaaaggttg tgagacaaca ccaagtcctg ggttcgcaat gaagaagttt
     3001 tcgttgccat cgccatcgat gaagccgacg tcttcgatag tgcggtccag gttcttgcga
     3061 gtgtagcggc cgctcagaac gatgtcgcgg cccagtccat actcagttcc cactgtgaat
     3121 tcagtttgcc gagcagcttt tagatccgga tccacgcgat tgtcggacgg atcgtttgaa
     3181 ggtacgcgga aatcaagtgg gccaaccagc gtatgtgcca aggtataagc cctggtgtaa
     3241 gtggtccagt ttggatcagc cagcgggaag aagtagcgca gcagcttatc gccaccgaat
     3301 gatccacgtg gtagctgata cttgaagcga tcaaagaact gaccgtagct cgcgaacacc
     3361 ttcatcctgc catctccgaa aacgtcgtag gcaaagccaa gacgtggagc aatcttgtcg
     3421 ccccagccaa acttgatagc ctgaccgttg ggggagaaag atggaacgtc ttcattctca
     3481 aagcgaacgc ccagattgag agtcaaccgg ttaatcggtt gccaactgtc ctgggcgtag
     3541 attgcagtgt tcgtactgcc t
//
LOCUS       DASUEX010000502         2283 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_524077, whole genome shotgun sequence.
ACCESSION   DASUEX010000502 DASUEX010000000
VERSION     DASUEX010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_524077"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            142..807
                     /locus_tag="VFV61_08410"
     CDS             142..807
                     /locus_tag="VFV61_08410"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4900539.1"
                     /translation="MLKVEHVSKEYPAPSGTLRIISDVSLALARGDAVSIMGPSGSGK
                     STLLYIMGALEPPSSGTVTLNGQNPFQLEQKALAAFRNKEIGFVFQDHCLLPQCSVLE
                     NVLTPTLVSRGNNNVSERAKELLEQVGLADRLDHRPAKLSGGEKQRVSLARALITKPH
                     LLLCDEPTGNLDHKSADVVASLLLELHRQQETILVVVTHSAELAARFPTRFELTDQHL
                     RQL"
     gene            807..>2283
                     /locus_tag="VFV61_08415"
     CDS             807..>2283
                     /locus_tag="VFV61_08415"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX4900540.1"
                     /translation="MTIFQLVKRNLLFYWRTNLAVVLGVATAVAVLAGALLVGDSVRA
                     SLRDLFLQRLGNTSYAIAGNGFFREQLAADIQTDTQFAQAGFTEMCPLIALEGNVTHE
                     ASKHRGSGIRVYGVDDRFWKFHRRNVAAPNNDEVFLSESLARELSANTGDSLLLRVEK
                     FSEIPIESLHSQKEDLGSTMRLQVRQILDARALGEFSLQPQQTEVRAVFVSLRRLQEE
                     LEQQGKANLILVSENSQKDAQQVGEATLGRLLKEKVTVEDYGLKLRALTDRQTISVES
                     EAGLLPETLAEAVQAAAQSSELAAAPFLSYLVNGIRTDEGREIPYSIVSSVGAEMMEV
                     MQHNESGHMPSCETFPPAGTQRSPTSQLPPILLTEWAGKDLQVKLGERVTLDYYVWLE
                     EGKLATRSAEFRVACIIPMDGLATDRDLVPDYPGISESESLSDWNPPFPIDLQRVRPQ
                     DEDYWKKYRTAPKAFVPLEVSQQLWRSRFGQLTSLRVQPKRG"
BASE COUNT          530 a          639 c          616 g          498 t
ORIGIN      
        1 ggtcaggaag agcggcttgc cgccggggct ttaaaattac caaggattaa gcgggggcaa
       61 gccacacctt ccggactgag cagttccgag ttgaattccg ggcttaaggc ggcgcactcc
      121 aaaggctaat tagggtttcc aatgcttaaa gttgaacacg tctctaaaga gtatccggcg
      181 ccgagcggca cgctccggat catctcagac gtctccctcg cactagcgcg cggtgacgcg
      241 gtctcgatca tgggaccctc cggcagcggc aagagcactt tgctctacat catgggtgcg
      301 ctcgaaccac ccagcagtgg caccgttacg ttgaacggcc agaatccttt tcaactcgag
      361 caaaaagcac ttgccgcgtt tcgcaataag gaaatcgggt tcgtattcca ggatcactgt
      421 cttctgccgc aatgctcggt gcttgagaac gtgctaacgc cgactctcgt ctcgcggggc
      481 aacaacaacg tctccgagcg cgccaaagaa ttacttgagc aggtcgggct cgcggatcgc
      541 ctcgatcatc gccctgccaa actatctggt ggcgagaagc aacgcgtttc actggcgcgg
      601 gcgctaatca caaaaccgca tttgctgctc tgtgacgaac cgacgggcaa tctcgatcat
      661 aagtcggctg acgttgtcgc ttccctgctg ctcgaactgc atcggcaaca ggagacaatt
      721 ctcgtggtcg tcacacacag cgccgaactg gccgcccgct tccccactcg tttcgagttg
      781 actgaccaac acttgcgaca gttgtgatga caatttttca actcgtcaaa cgcaacctgc
      841 tcttttactg gcgtaccaat ctcgctgtcg tgctgggcgt cgctacggca gttgcagtgc
      901 ttgcgggtgc tttgctcgtc ggcgactcgg tacgcgccag cctgcgcgat ttgtttctcc
      961 agcgattagg taatacgtct tatgccatcg ctggtaacgg attcttccgc gaacagctcg
     1021 ccgcggatat ccagaccgat acccaattcg cccaagccgg atttacggag atgtgcccgc
     1081 taatcgcgct cgaaggaaat gtcacccatg aagccagcaa acaccgtggc tcgggaattc
     1141 gagtttacgg agtagacgat cgcttttgga aattccatcg ccgaaatgtg gccgctccca
     1201 acaacgacga agtatttctc agtgaaagtc tggcgagaga attaagtgca aacacggggg
     1261 attcgctact ccttcgagtt gagaagtttt ctgaaatccc catcgagtcg ttgcacagcc
     1321 agaaggaaga cctgggaagc acaatgcgct tacaggtgcg tcagattttg gacgcccggg
     1381 ccctgggaga gttctcactt cagccccagc aaaccgaagt tcgcgcggtc ttcgtatccc
     1441 tgcgtcgctt gcaggaagag ttagagcagc agggaaaagc caacctcatt cttgtttccg
     1501 agaattctca gaaggacgct caacaagtcg gcgaagctac cctcgggcga cttctaaaag
     1561 aaaaagtaac cgttgaagac tacggcctaa agttgagagc cctgactgat cggcaaacaa
     1621 tttcggttga gagtgaggca ggattgctgc cagaaacttt ggccgaggct gttcaggcgg
     1681 ccgcccagtc ttccgagtta gctgcagcac cctttctgtc ttatctcgtg aatggcatcc
     1741 gcaccgatga gggacgggag attccgtact ctattgtaag cagtgtggga gcggagatga
     1801 tggaagtaat gcagcataat gaaagtgggc atatgcccag ctgcgaaacg ttcccacctg
     1861 cggggacaca acgctcacca acttctcaac tcccaccaat cctactgacc gaatgggcag
     1921 gcaaagatct ccaggtgaag ttaggagagc gggttacact cgattactac gtctggctgg
     1981 aagaaggaaa gcttgcgacc cggtcggctg aatttcgggt ggcctgcatc attccgatgg
     2041 acggcctcgc aaccgacaga gacttggtcc ctgattaccc cggcatttcc gaatcggaaa
     2101 gtctaagcga ttggaacccg ccgtttccga ttgacctgca acgcgttcgt ccgcaggacg
     2161 aagactattg gaaaaaatat cgcacggcgc caaaagcttt tgtgcctctc gaagtttctc
     2221 aacagctttg gaggtcgcgc tttggccaac tcacttcgct tcgcgttcag cctaagcgcg
     2281 ggc
//
LOCUS       DASUEX010000503         2419 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1467365, whole genome shotgun sequence.
ACCESSION   DASUEX010000503 DASUEX010000000
VERSION     DASUEX010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1467365"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1156
                     /gene="rseP"
                     /locus_tag="VFV61_08420"
     CDS             <1..1156
                     /gene="rseP"
                     /locus_tag="VFV61_08420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEX4900541.1"
                     /translation="PLEGAGAQDIPEHERFNLRPRWQRILVALAGPVMNVLTALAIPL
                     AGALMFGIPATPAPVVDALSRGGAAEIAGMQVGDRIVSFNGNEHPKWETIRGDALLSP
                     GQQLPLVVERNGQRVALTITPTAHVEDGETAGFLDFVPDYGDVPVVVREVEPSTPAAE
                     AGLQPRDRITGIGGHPVGSAEQVTQFIKDHKGQPITLTVDRNGERKDITATPRKLSDG
                     RERLGFRPDEEVPLQRVGLIAGTAYAVNTNLEILRLTGKALGQVFTGQRSARNTLSGP
                     IGIYNAAATSVERLGWAGVFGMLGFLSLNLGIFNLLPIPVLDGGAIFLLVIEGLLATV
                     GLTISVTVRDRIQQVGFVMVLLLMVFVISNDVVKLFSSGPEKPAATAPAK"
     gene            1246..2352
                     /gene="ispG"
                     /locus_tag="VFV61_08425"
                     /gene_synonym="gcpE"
     CDS             1246..2352
                     /gene="ispG"
                     /locus_tag="VFV61_08425"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF001540.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEX4900542.1"
                     /translation="MRTSRPVKIKDIQIGGGAPVVVQSMTKTDTADVGATVAQIEEMV
                     RAGCEIVRLAVPDNDAAYALKEIRKRVPDVPLVADIHFHYKLALLALEAGIDKLRLNP
                     GNIGKHERIVEVVRAAQAQKVPIRIGVNGGSLEKDLLARYGTATPEAMVESAMRHIRI
                     LEDLGFTDTIISLKASDVERTVAAYRLLAQKVDYPFHLGVTEAGTAFTGTIKSAIGLG
                     ILLHEGIGDTIRVSLAAEPQEEVRVAWEILKSLELRKRGVTVVACPTCGRLDVDNFVE
                     IVTEIERRLSHIQEPLHLSIMGCAVNGPGEAHDSQLGITFGRGVGMIFKDGIPMRKVA
                     GENIVEAFVTETEKLLAEVQEVNQAKEKELVAAE"
BASE COUNT          522 a          627 c          714 g          556 t
ORIGIN      
        1 cccgctcgaa ggtgccggtg ctcaggacat ccccgagcac gaacgattta atctgcgacc
       61 gcgttggcaa cggattctcg tcgcccttgc tggtccggta atgaacgtat tgacggcact
      121 ggcaattccg cttgccggag cgttgatgtt tggcattccg gcaactccgg cgccagtcgt
      181 cgatgcactc tcgcgcggcg gagccgctga gatagcggga atgcaggtag gcgatcgcat
      241 tgtttctttt aatgggaatg agcacccgaa gtgggaaacg atccgtggcg atgctttgct
      301 ttcccccggc cagcagttgc cgctcgtggt tgagcgcaat ggccagcgag tggcattgac
      361 aatcacaccg accgctcacg ttgaagatgg cgaaacagca gggtttctcg atttcgttcc
      421 tgactacggc gatgtgccgg tagttgttag agaagttgag cctagtacgc ctgctgctga
      481 ggcggggttg cagcctcgtg atcggataac agggatcggt ggtcatccag tcggcagtgc
      541 tgaacaggtg acccagttta ttaaagatca caaaggtcag cccattaccc ttaccgtaga
      601 tcgcaatggg gagcggaaag acatcaccgc aacgccgcgc aagctttccg atggccgcga
      661 gcgcctaggt tttcgtcccg atgaagaggt tcccttacaa cgggtcggtc taattgccgg
      721 taccgcttac gcggtaaata ctaacttgga aatcctgcgc ttgacgggga aggcgctggg
      781 acaggttttc acaggccaac gctccgcccg caacacactt tccgggccga ttggcattta
      841 caacgccgct gctacttcgg ttgagcggct gggttgggct ggtgttttcg gcatgctcgg
      901 gttcctgagc ttgaatctcg gtatcttcaa cctgttgccc attccggtgt tggacggcgg
      961 cgctatcttc ttattggtga tcgaaggctt gctggccaca gtgggtctga ccatctcggt
     1021 aactgtgcga gatcgcattc agcaggtggg cttcgtaatg gtgctgctgt tgatggtgtt
     1081 tgtgatttcc aacgacgttg taaaactgtt ctcgagcgga cctgagaaac ccgccgccac
     1141 agcgccggcg aaataaatca tgtccaaagt tcaacgtcca gagtccaacg ttagctctag
     1201 tcacaggctt tgggccttgg accttgggcg ttggacaaag ttgtaatgcg aacctcccgt
     1261 ccggtaaaaa tcaaagacat tcaaattggt ggcggcgccc cggtcgtggt gcagtctatg
     1321 accaagactg acaccgctga cgttggcgcc acagtcgcgc agatcgaaga gatggtgcgg
     1381 gccggttgtg aaatcgtgcg cctcgccgtt cccgataatg atgccgctta cgcgctgaaa
     1441 gagattcgca agcgcgttcc ggacgtgcca ctcgttgcgg atatccactt tcactacaag
     1501 ctcgccttgt tagctctcga agcaggtatc gataagctgc gtctcaatcc cggcaatata
     1561 ggcaagcacg aacgcatcgt tgaagtggtg cgcgccgcgc aagctcaaaa agttcccatt
     1621 cgcattggtg tgaatggcgg ttcgctggaa aaggatctgc tggctcgcta tggcacagcg
     1681 acgccggaag caatggtcga atcagcaatg cggcacattc gcatcctcga agacctcggg
     1741 tttacggata ccatcatttc tttaaaggct tccgacgtcg agcgtacggt tgcggcctat
     1801 cgcctgctgg cgcagaaagt tgattatcct ttccatctgg gcgtgacgga ggccggaact
     1861 gcctttaccg gcacgattaa gtcggcaatc ggcttgggaa tcctgctaca cgaaggcatc
     1921 ggtgacacga ttcgcgtttc gctggccgca gaaccgcagg aagaagttcg cgtcgcgtgg
     1981 gaaatcctca aatcgcttga gttgcgcaaa cgcggagtta ctgtggtcgc ctgtcctacg
     2041 tgtggtcgac tcgacgtgga caactttgtc gagatcgtaa cggaaattga acggcgcttg
     2101 tctcacatcc aggagccact gcatctgtcc attatgggct gcgctgtgaa tggtccgggc
     2161 gaagcacatg attcacaact gggcattact ttcggtcgcg gcgtggggat gatctttaag
     2221 gatggcatac caatgcgcaa ggtagcgggc gaaaacatag tggaggcttt tgtgacggag
     2281 actgaaaaac ttctcgccga agtgcaagaa gtcaatcaag caaaagaaaa ggaactggtg
     2341 gcggccgagt agtgcaaagc agtttgccgt taagattcaa ggaccgccat tcggcggtct
     2401 tttcatgtta ggcaacagt
//
LOCUS       DASUEX010000504         2652 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_423164, whole genome shotgun sequence.
ACCESSION   DASUEX010000504 DASUEX010000000
VERSION     DASUEX010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423164"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            241..1746
                     /locus_tag="VFV61_08430"
     CDS             241..1746
                     /locus_tag="VFV61_08430"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX4900543.1"
                     /translation="MSHLLIVDDELGMRQFLTHLFQREGHDVRVAESGRKAMSLLREE
                     PADLIISDVKMPDMGGIELLRAARELLPHVEVVLMTAFANVETAREAFLLGAYDFVQK
                     PFDNDLLKEVVARALEKISLVKEKDAILKVLGQGQRKQAQLGNIIGRSGSMQALFQMI
                     ETVAQVQATVLVTGESGTGKELVARAIHDLGPRAEKPFVSINCGAFTETLLESELFGY
                     VKGSFTGANANRKGLFEAANSGTIFLDEIGEMSPAMQVKLLRVLQERKLRPVGATEET
                     TVDTRVIAATNRDLASMVKEGSFREDLFYRISVIPIELPALRQRLEDVADLANHFIAK
                     FCAQSGRSLGISEAAMRLLEDYSWPGNVRELEHTIERAVALETSDAIQPERLPEKITS
                     YNPHRVAAAFNFPEEGLNLTAYLDQLEKTYLLEALQRSGGNQTNAATLLHLSVRSLRH
                     LLDKHGIRALTAQMRDERRSPDSTPRRRTTDPSERRREEDQAEFAAGAAEK"
     gene            2004..2486
                     /locus_tag="VFV61_08435"
     CDS             2004..2486
                     /locus_tag="VFV61_08435"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX4900544.1"
                     /translation="MKTNKSQAGFSLIELLIVVAIIGIIAAIAIPNLLASKRAANEGS
                     AVASMRTITSAEATYAATTGSGQYGTLPNLAGQGLVDSVLGGAAGTKSGYSFVSTPSG
                     TGTAADPWLYLSTASPQAVGGVTATGTRNFASDPTGVIYSAAGSTTPMSAATGTAIGN
                     "
BASE COUNT          605 a          786 c          700 g          561 t
ORIGIN      
        1 gggccgcggc acaacgatta cgctggaatt gccggcccgg gcgaagaatc aattgccagt
       61 gggcaatagt ctgtagtcag tagtcagtgg tccgtggtcc gtggtccgtg gtcagttgtt
      121 cagcgatcgc tcaaacaaaa agtcactaag aagaataatt tttagacgca aagtatgtct
      181 accaaccacc actaactact aactactaac tactgactac taactactga ctacttaact
      241 atgtcccacc ttttaatagt cgacgacgaa ttaggcatgc gtcagtttct gacgcatttg
      301 ttccagcgcg aaggtcacga tgtcagagtg gcggaaagcg gtcgcaaggc catgtctttg
      361 ctccgcgaag agccggctga cctgatcatc tccgacgtaa agatgccgga catgggcggc
      421 atcgaattgc tgcgggccgc gcgtgaactt ttgccgcacg tggaagtggt tctgatgact
      481 gccttcgcca atgtcgagac cgcacgcgaa gcctttctgc tcggcgctta cgactttgtc
      541 caaaagccat tcgacaatga cctgctgaaa gaagttgtcg ctcgtgccct tgaaaaaatc
      601 tcgctggtaa aagaaaagga cgcgatcctg aaagtgctgg gccagggtca gcgaaaacag
      661 gcgcaactcg ggaacattat cggccgctcg ggaagtatgc aggcgctctt ccaaatgatt
      721 gaaacggtgg cccaggtcca ggctacagtg cttgtaacgg gagaatcggg cactggaaaa
      781 gaacttgtcg cacgcgcgat tcacgacctc ggcccgcgag ccgaaaagcc tttcgtttcg
      841 atcaactgcg gcgcgttcac tgaaacactg cttgagtcag agctgttcgg gtacgtcaaa
      901 ggttcattca ctggggcgaa cgccaatcgc aagggccttt ttgaagcggc aaacagcggc
      961 acaatttttc tggacgagat cggcgagatg tcgcccgcga tgcaggtaaa gctgttacgc
     1021 gtactgcaag aacggaagct gaggccggtg ggagccactg aggaaaccac tgtcgacacg
     1081 cgtgtgatcg cagccacgaa tcgcgatctc gcgagcatgg taaaagaagg atcgtttcgc
     1141 gaggatctct tttatcgcat ctcggtcatt cctattgagc tacccgcgct acgccagcgg
     1201 ctcgaagacg ttgccgacct ggctaatcac ttcatcgcca agttttgcgc gcagtcgggc
     1261 cgcagcctgg gaatcagcga ggcagcaatg cgcctattgg aagactattc atggcccgga
     1321 aatgtccgcg aactcgaaca cacgattgaa cgcgccgtcg cgctcgagac cagtgatgcg
     1381 atccagcccg agcgtctgcc tgagaaaatc acgagttaca acccccatcg agtggccgcc
     1441 gcctttaatt ttcccgagga gggattaaac ctcacggcct atctggatca attggaaaag
     1501 acctatctgc ttgaggctct gcaaagatcc ggcggcaacc agaccaacgc tgccacgctc
     1561 ttgcaccttt ccgtacgttc cctgcgccac ctcctcgata aacatggcat tcgcgccctc
     1621 acagcacaga tgcgcgatga acggcgcagt cctgattcga cgcctcggcg ccgcacgaca
     1681 gatcccagcg aacgacggcg tgaagaagat caggccgagt ttgccgccgg cgccgccgag
     1741 aaataagctc aacactacct gctcccacaa ttcgtcagcg cctgctgacg taaattgtca
     1801 ggaactcaat cgactcattt ccagtttttt ctgctgcgcc aaatctctcc gattttgcgc
     1861 cggataggcc tttttcgctc cttggcacga accttgtagt tagagcagcc gacaacttcg
     1921 cgctctgcgc ggcctttaga gaaagaagcc gttggcgcga caaacagcaa atcaatttct
     1981 cttaaggagc atgaccaaaa gaaatgaaaa ccaataaaag ccaagccggc ttctcactga
     2041 tcgaactcct catcgtggtc gcgatcatcg gcatcatcgc cgccatcgct atccctaacc
     2101 tgctcgcttc taaacgcgcc gccaacgaag gttcggctgt cgcgtccatg cgcaccatca
     2161 ccagtgccga agcgacctac gctgccacga ctggtagcgg acagtatggc acccttccga
     2221 acctcgctgg ccagggactg gttgacagcg tccttggtgg tgccgctggc accaagagcg
     2281 gatactcctt cgtttccacc ccgtctggca ccggcacggc tgctgatccc tggctctatc
     2341 tctcaaccgc ttcaccgcag gcagttggtg gcgtgaccgc aaccggtact cgcaacttcg
     2401 cgtctgaccc gactggcgtt atctattcag ccgccggcag caccactccg atgtccgccg
     2461 ctaccggcac cgccatcggt aactaattca ctcctctcct tggtcctcac cttggctccc
     2521 ccgggggcgc ggagcgaaac agctcccgcc cccgggtctt ttttgatgat tgagtttgtt
     2581 atggaaaaag ccgtcaaagc gctgaaggat ccgcggtggt tcatcgccgt gttatggccg
     2641 ctggctttgg cc
//
LOCUS       DASUEX010000505         7117 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_423378, whole genome shotgun sequence.
ACCESSION   DASUEX010000505 DASUEX010000000
VERSION     DASUEX010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7117
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423378"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..886)
                     /locus_tag="VFV61_08440"
     CDS             complement(<1..886)
                     /locus_tag="VFV61_08440"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
                     /protein_id="HEX4900545.1"
                     /translation="MRIAILGTRGIPASYGGFETFAEHLSTRLVARGHDVTVYCRAHY
                     VSPRQLESHGVRLKVLPTIRHKYLDTVVHAFLSALHAVPARFDAALICNAANAPFASI
                     LRGTGTPVAINVDGLEHKRKKWNWLGRKYYLLAERLSTILPNETVTDACVIQDYYAAK
                     YNARSTMIAYGAEVERKPDRPVVRRWRVEPNRYVLYVSRLEPENNARMVIEAFKKVRT
                     AYRLLVVGDAPYAHEYINDLKERARGDRRIIFTGFVFGQDYRALQQNAYCYVHATEVG
                     GTHPALLEAMGFGNCVLTL"
     gene            complement(913..2076)
                     /locus_tag="VFV61_08445"
     CDS             complement(913..2076)
                     /locus_tag="VFV61_08445"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEX4900546.1"
                     /translation="MRIAIDAHSVGTGLGGNESYATNLIEALAAIDSVNEYTLYVTRP
                     EAVKTFSDRWPNFTVRQTRPHTPLIRIPLTLSAELRKNPVDVLHVQFTAPPFAPCPVV
                     VSIHDLSFEHLPETFKRRSRAQLRLTVRRSARKATHILALSEHARADIVATYGIAPER
                     VTAIPLAAADRFRPITDEKELQRVRHTYGIAGDYILTVGSIQPRKNLRRLISAYTRLR
                     REAPEATHPSLVIVGRQAWLFGDTLRAIRDSGFADSIILTGYVPDADLPALYSGARCF
                     VYPSFFEGFGLPPLEAMKCGVPVIAGNRTSLVEVVADAGILVDSLDSDAIASALSRVL
                     NDRELREELSVKGLNRSRLFRWQETAKRTLEVYERAVDNKRAHILETREQRLR"
     gene            complement(2122..2517)
                     /gene="rplS"
                     /locus_tag="VFV61_08450"
     CDS             complement(2122..2517)
                     /gene="rplS"
                     /locus_tag="VFV61_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004415194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEX4900547.1"
                     /translation="MNRLDGIEKTQLRDKVPQFQPGDTVRVHVRIKESETKERLQAFE
                     GVVIARKHGGARETITVRKTSFGVGVERIFPLHATIVDHIEVIKRGRVRRAKLYYLRE
                     LRGKAARIRERDTRGQQAKDAQASAAKVK"
     gene            complement(2598..3299)
                     /gene="trmD"
                     /locus_tag="VFV61_08455"
     CDS             complement(2598..3299)
                     /gene="trmD"
                     /locus_tag="VFV61_08455"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: protein motif:HMM:TIGR00088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEX4900548.1"
                     /translation="MLRFDIVTIFPDFFRETFDYGIIRRARNAKLVTIEAHDLRAWTT
                     DKHHSVDDRPFGGGDGMVFKPEPVFAAVAELTGKNERVDYPAATRVVLLSPQGRVFTQ
                     QLAQDFASGATQVVLICGRYEGVDERVVEGLVTDEISIGDYVLSGGEPAALVVIDAIV
                     RLLPGALGSETSATSESFSEGLLDYPHYTRPPELRGLKVPDVLLTGNHAEIARWRREK
                     ALEKTRRNRPDLLKN"
     gene            complement(3307..3843)
                     /gene="rimM"
                     /locus_tag="VFV61_08460"
     CDS             complement(3307..3843)
                     /gene="rimM"
                     /locus_tag="VFV61_08460"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEX4900549.1"
                     /translation="MKQSLDELDLVIVARAVKTRGLKGELVAELLTDFPDRFADISSL
                     IAVGPQGIRKSVELESHWLQQGRVVLKFAGYDSIERAKELVGFDFAVAEGERVELEDG
                     YFYDWQLEGCLVESNDGVKIGTVKEVRRLGGEIDMLAVEDADGVSRLIPLVEGIVLEV
                     DIAGRKIRIDPPQGLLEL"
     gene            complement(3840..4070)
                     /locus_tag="VFV61_08465"
     CDS             complement(3840..4070)
                     /locus_tag="VFV61_08465"
                     /inference="COORDINATES: protein motif:HMM:NF024483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEX4900550.1"
                     /translation="MKETVEMIVKALVDDVDAVDVREIDRNGTTLLEVRVAPTDMGKV
                     IGKQGRTVRALRSLAHAVSLKRKHRFVLEIVE"
     gene            complement(4120..4386)
                     /gene="rpsP"
                     /locus_tag="VFV61_08470"
     CDS             complement(4120..4386)
                     /gene="rpsP"
                     /locus_tag="VFV61_08470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEX4900551.1"
                     /translation="MLAIKLMRTGAKKRPSYRVVVKEKQSKRDGAYLENVGTYDPTRE
                     PAEIKLKMDRVRYWIGKGAQPTDTVGRLIKASSKAEAAAQAATV"
     gene            complement(4417..5895)
                     /gene="ffh"
                     /locus_tag="VFV61_08475"
     CDS             complement(4417..5895)
                     /gene="ffh"
                     /locus_tag="VFV61_08475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEX4900552.1"
                     /translation="MFESLSDKLKRTLKNLRGEGVLTKEHVEAALREIRLALLEADVN
                     YKVAKDFIASVKEKAEGAQVWQDLKPSEQVVKIVYDELVDLLGGQSSRLVFTRQIPNV
                     VMIVGLQGSGKTTSTGKIARWLAQNQDRKPLLVSVDVYRPAAREQLRVIAKATGQQIF
                     EQPETNDPLTLVREAYKHAQQTGFDTLLIDTAGRLHIDDDLMVELERIKQETHPVEIL
                     FVADAMTGQDAVRSAEEFHKRVGITGVVLTKMDGDARGGAALSIKQVIGQPVKFVGVG
                     EKYDALDPFYPDRIAQRILGMGDVLSLIEEVQSKVDQDEAEAQLKKIQKNEFTLDDFR
                     SQLRQVKKLGSFSKIMKLLPDQLLGGMGMPQLNDEQTKLMEKELKRTESIIDSMTWQE
                     RTDHKILNANRRRRIARGSGTSVAEVNQLVKQYTEMRQMMRQLSSSGMFGGGGLKSKM
                     MRRMTGMPDLSGMGMEGNGGDQQLPAGPSRKKLKKKKRKKRR"
     gene            6229..>7117
                     /locus_tag="VFV61_08480"
     CDS             6229..>7117
                     /locus_tag="VFV61_08480"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX4900553.1"
                     /translation="MAKRVRSGPGGPETDVTADGRSVVDLADARVRLRSKDPSNEALI
                     EELDEVRSLLDQGLSTEARTRLTSLISAARNNPTVLALARCALSAALEMQGRYRESLT
                     AVAMYETPESQAKLDPETVCSLRVQIGLAHNYNGDHPKAISILKAALREFSEGGSEAR
                     LGPIYGALARVYRTISEYPIARDYAQRALDQYRQTGDWRGLAEAYFGIAIADTQEGNY
                     ESGLENFEQALKLIGDNPASYSLGRIYANMAGACWFLKRPLEGIRFLEKAINYYERTD
                     HKTSAADGYNNLGINLILIG"
BASE COUNT         1605 a         2076 c         1787 g         1649 t
ORIGIN      
        1 cgagcgtcag gacgcagttt ccgaagccca tggcttcaag caaagccgga tgggttcccc
       61 cgacttcggt tgcgtggacg taacaatacg cgttctgctg caaggcacgg tagtcctggc
      121 cgaaaacgaa accggtaaaa attatccgcc gatcgcctcg agctcgctct tttaagtcgt
      181 tgatgtactc atgcgcgtaa ggggcgtcgc caaccaccag caatcgataa gcagtgcgaa
      241 ctttcttgaa cgcctcgata accatgcggg cattgttttc cggttcgagg cgcgaaacat
      301 aaagcacgta gcgatttggt tcaacgcgcc agcgtcgcac tacaggtcga tctggttttc
      361 tttcaacctc ggccccataa gcgatcatcg tcgagcgcgc gttgtatttg gccgcgtagt
      421 aatcttgaat cacgcaggcg tcggtgaccg tctcattcgg caggattgtg gagagacgtt
      481 cagcgagcag gtaatacttt cgtcccagcc agttccattt cttacgtttg tgctcgagcc
      541 catcaacgtt tattgccacg ggagtgcccg tgccccgcaa aattgaagca aagggagcat
      601 tggccgcgtt gcagattaac gccgcatcaa aacgcgcagg gacggcgtgc agcgcggaga
      661 gaaaagcgtg aacgacggta tctaagtact tgtgcctgat tgtgggaagc actttcaagc
      721 gaaccccgtg ggactcaagc tgtcgcggag aaacgtaatg ggcgcggcag taaaccgtca
      781 catcgtgacc gcgcgctacc agccgcgtag acaggtgttc cgcaaaagtt tcaaagccac
      841 cgtagcttgc cggaattccg cgggtgccga ggatcgctat acgcatactt ttttcagtag
      901 ctaatggggg tgctaacgaa gtctttgttc ccgcgtctca aggatgtggg cacgtttatt
      961 atcgacggca cgttcgtaaa cttcaagagt tcgttttgcg gtctcttgcc atctgaacaa
     1021 gcgcgaacga ttcaagcctt tgacgctgag ctcctcacga agctcccgat cattcaacac
     1081 gcgtgacaac gccgacgcga tggcgtccga gtccagcgaa tccacaagta tcccggcatc
     1141 cgcaacaact tccaccaggc tggtgcgatt tccggcaatt accggcaccc cacatttcat
     1201 cgcctctaaa gggggcagac cgaacccttc aaaaaaagag ggatacacaa aacagcgggc
     1261 gccggaataa agcgcgggaa gatcagcgtc aggtacatag cctgtcagga ttattgaatc
     1321 ggcaaaaccg ctatcgcgaa tcgcgcgcag tgtgtctcca aataaccaag cctgtctacc
     1381 aacaattacc aggctcggat gcgtcgcttc tggagcctct cggcgcagcc gcgtgtacgc
     1441 tgaaatcaag cggcgcaaat tcttgcgtgg ctgaatagat ccgaccgtga ggatgtagtc
     1501 tccagctatt ccgtaagtat gtcgcacacg ttgcagttct ttttcgtcag tgattggtcg
     1561 aaagcgatcg gcggcggcga gcggaattgc agtgacccgc tcgggcgcaa tgccataggt
     1621 tgcgacaata tcagctcgcg catgttccga gagcgccaaa atgtgagtgg ccttgcgcgc
     1681 cgaacgccgc accgttaacc gcagttgggc gcggctccgg cgcttgaaag tttcaggaag
     1741 atgttcaaaa gagagatcgt gaatactgac tacgaccgga caaggcgcga aggggggagc
     1801 ggtaaactga acgtgcaaca catcaacagg attctttctc agctcagcac tgagcgttag
     1861 cggaattcgg attagggggg tgtgcggacg ggtctgacga acagtgaagt ttggccagcg
     1921 atctgaaaac gtcttaactg cttccgggcg ggttacgtag agggtgtact cgttgactga
     1981 atcgatcgct gccagcgctt cgatcaagtt agtagcgtaa ctctcgttcc cgcccaagcc
     2041 ggtgccgacg gaatgggcat caatagcgat ccgcaaaaat cagctcccgg tcacctctga
     2101 accacggcga aggatccgcg actatttaac cttggcagcg gaggcctgag cgtccttcgc
     2161 ttgctggccg cgcgtgtcgc gttcgcgaat gcgggccgct ttgccgcgca actcgcggag
     2221 gtaataaagt tttgcgcgcc tgactcgacc ccgcttaatc acttcaatgt gatcgacaat
     2281 agtggcatga agcggaaaga ttctttcgac gccgacacca aagctggtct tccgaacggt
     2341 aattgtctca cgagcgccgc catgcttacg cgcgatgact acaccttcaa aagcctgcag
     2401 ccgttctttg gtttcggatt ctttgatccg aacgtggacg cgtacggtgt cgcccggctg
     2461 aaattgcggc accttgtcgc gcaattgtgt tttctcaatc ccgtctagtc gattcatttc
     2521 aatcactcca ctaaaaactc gaattccgaa atcagccaac cctcaacccg gcagggcaag
     2581 ctcctaaaag tcgcctacta attcttcaac aaatccggac gattccgtcg cgtcttctct
     2641 aacgcctttt cccgtcgcca gcgcgcaatc tctgcatgat tgccggtcaa taaaacatct
     2701 ggcactttca aaccacgcag ttccggcggt cgcgtgtaat gcggatagtc cagtaaacct
     2761 tctgaaaacg attcggacgt tgctgacgtt tcgcttccca acgctcccgg caataaacga
     2821 acaatcgcgt caatcacaac cagggccgcc ggctcgcctc ccgaaagtac ataatcaccg
     2881 attgaaattt catcggtaac aagtccttcc accactcttt catccacacc ctcataccgg
     2941 ccgcaaatca aaacaacctg tgtcgcgccg ctcgcaaaat cttgcgccag ttgctgtgtg
     3001 aatactcgcc cctgaggcga taacaatacg actcgcgttg ctgccggata atcaacccgc
     3061 tcgttcttac ctgtcagctc tgcgacggca gcaaagaccg gctcaggctt gaaaaccatg
     3121 ccgtcgccac cgccaaaagg tcgatcatca acggagtggt gtttgtcggt ggtccaggcg
     3181 cgcaagtcgt gcgcttctat cgtcaccaac ttcgcattac gggctctgcg aataattcca
     3241 tagtcaaacg tctcgcgaaa gaagtcggga aaaatcgtga ctatgtcaaa gcgcagcacg
     3301 taaaacctac aattccaaca acccctgcgg cggatcgatc cggatcttcc gtcccgcaat
     3361 atcgacttcc agaacgatcc cttcgacgag gggaattaag cgactaactc cgtcagcgtc
     3421 ttcaaccgcc agcatgtcaa tctctccgcc gagtcgcctc acctctttga cagtgccaat
     3481 cttcacgcca tcattgcttt ccaccaggca gccttccaat tgccagtcgt agaagtatcc
     3541 gtcttcaagc tcaactcgct ccccttcggc aacagcgaag tcaaaaccaa cgagctcttt
     3601 ggctctctca atcgaatcgt agcccgcgaa ctttagaacc acacggccct gctgaagcca
     3661 gtggctttca agttcaacgg acttgcggat accctgagga ccaaccgcta tcaaggagct
     3721 gatgtctgcg aaacgatccg gaaagtccgt aagcaattcg gcgaccaatt cgcctttaag
     3781 acctctcgtc tttacggcgc gagcgacgat gaccaaatcg agctcgtcta agctctgctt
     3841 cattcgacaa tttctaaaac gaatcgatgt ttacgcttca aactcaccgc gtgcgccaac
     3901 gatctgagcg cgcgcactgt gcgaccctgt ttcccaatta ctttgcccat gtcagtaggc
     3961 gccactcgta cttcgaggag ggtcgtaccg ttgcggtcaa tctcgcgcac gtcaacagcg
     4021 tcaacatcgt ccacgagcgc cttcacaatc atttcgacgg tttctttcat gtagcgttcg
     4081 cgacctaacg cgtgtgccga actgcccgac gaaatcttac taaactgtgg cggcctgcgc
     4141 cgctgcctct gctttgctgc tggccttgat gagccgcccg acggtgtctg tcggttgggc
     4201 gcctttaccg atccagtaac gcacgcgatc cattttgagc ttgatctcag caggctcacg
     4261 cgtcgggtcg tacgtcccga cattctcgag atacgcgcca tcgcgctttg actgtttctc
     4321 cttcaccacc acgcggtagg aaggacgctt cttcgcgccc gtgcgcatca atttaatagc
     4381 taacaagtgc ttctcctccg taacatcaac gcttcgttat cttcgttttt ttcttttctt
     4441 cttttttagt ttctttcgtg aaggcccagc gggtaattgt tgatcgccgc cgtttccttc
     4501 catccccatt ccacttagat ccggcatgcc ggtcatgcgc cgcatcattt tacttttcaa
     4561 cccaccgcca ccgaacattc ccgagctgct gagctggcgc atcatctggc gcatctccgt
     4621 gtactgcttc accagttgat taacttcggc taccgacgtt cctgaccctc tcgcgatacg
     4681 ccgcctgcgg ttcgcattta gaatcttgtg gtcagtgcgc tcctgccagg tcatcgagtc
     4741 gataatcgac tccgttcgct tcagctcttt ttccatcagc ttggtctgtt cgtcgttcag
     4801 ctggggcatg cccattccgc ccaagagttg atcgggcaac agcttcatga tcttcgagaa
     4861 agagccaagc tttttgactt gccgcaactg cgagcgaaag tcatccagtg tgaattcatt
     4921 cttctggatc ttcttgagct gcgcctcggc ttcatcctga tctacttttg actgaacctc
     4981 ttcaatgagc gataaaacgt cgcccattcc cagaatccgc tgggcaattc ggtccggata
     5041 gaaaggatca agcgcgtcgt acttttcgcc aacgccgaca aacttcaccg gctggccgat
     5101 aacctgtttg atggagagtg ccgcaccgcc gcgggcatcg ccatccatct ttgtgagaac
     5161 gacgccggtg atgcctacgc gtttgtgaaa ctcttctgcg gaacgaaccg catcctgacc
     5221 ggtcatcgcg tccgccacaa acagaatctc aacgggatgc gtctcctgct tgatgcgctc
     5281 gagctccacc attaaatcgt cgtcgatgtg cagccggccg gcggtgtcaa tcagaagggt
     5341 gtcgaaaccg gtctgttgcg cgtgcttgta agcttcgcga acgagggtaa gcggatcgtt
     5401 cgtttcaggt tgttcaaaga tctgctggcc agtcgcttta gcgatgaccc ttaactgttc
     5461 gcgcgcagcg ggacgataaa cgtcaactga aaccaggagc ggctttcgat cctgattctg
     5521 agccagccag cgagcgattt tcccggttga agttgtcttg ccggaccctt gcagtccgac
     5581 aatcatcacc acattcggaa tctgcctggt aaaaaccagg cgtgacgact ggccgccaag
     5641 caagtcaaca agttcgtcgt aaacgatctt gacgacctgc tctgagggct taagatcctg
     5701 ccaaacctgg gcgccttcgg ccttctcttt gaccgacgcg ataaagtcct tagcgacttt
     5761 gtaattgacg tcggcttcca acagagcgag ccgaatttcg cgcagggccg cctcaacgtg
     5821 ttccttcgtg agcacgcctt cgccccgcag attcttgagc gttcgcttta gcttgtccga
     5881 cagcgactca aacatacatc cctaggcgaa gttccgccca tacgacggaa acggcaagaa
     5941 tagcgacgcg tcttaggagt gtcaagtttg cacctgtgct aagttttact cgtacttcga
     6001 gttaacggct agtgaacttc ttgagccaag acccaattga actcgctctt ttcggcactt
     6061 tgcaacagga actctgtaga cgcccttttg agcatgtgtt aaatttaact cgtttagaat
     6121 taacgagaag gcgtcgcggc caaacaggcg aaacttccaa ccttccgctc ttcacagcag
     6181 tagcgcagta tgaaacaagt cgttaattac gactttacag aaggttaaat ggctaaacgt
     6241 gtgcgctccg gacccggcgg tcctgaaaca gatgtgactg cagatgggcg aagtgtggtt
     6301 gacctcgccg atgcccgcgt tcgtctgcgc tcaaaggatc cctctaatga agcgctaatc
     6361 gaagaacttg acgaagttcg ttcccttctc gatcaaggac tttccactga ggcccgaacg
     6421 cggctcactt ccctgatctc ggcagctcga aataatccga cggtattagc cctcgcccgc
     6481 tgcgcgctat ctgccgccct cgaaatgcaa ggcaggtatc gagagtcgct aactgcggtc
     6541 gcgatgtatg aaaccccgga gtctcaagcc aagctcgatc cggaaaccgt ttgcagcctg
     6601 cgcgtgcaaa tcggactcgc gcacaactat aacggggatc atccgaaggc aatttcgatt
     6661 ttgaaagcgg cgctgcgcga gttttccgag ggcggttccg aagccaggct cggtcccatc
     6721 tacggcgccc tcgctcgagt ttatcgcaca atcagcgagt atccgatcgc gcgcgactat
     6781 gctcagcgcg ctcttgatca gtatcggcag actggggatt ggcgcggcct cgctgaagcc
     6841 tattttggca tcgccatcgc cgatacgcag gaagggaact acgaatcagg cctcgagaac
     6901 ttcgaacaag ctctcaagct aatcggtgat aatcccgcta gttattccct cggtcggatt
     6961 tacgccaaca tggccggcgc ctgctggttc ctcaaacgac ctcttgaggg cattcgtttt
     7021 ctcgagaaag ctatcaacta ctacgaacgc actgatcaca agacgagtgc ggccgatggg
     7081 tacaacaacc tcgggattaa cctgattctc attggac
//
LOCUS       DASUEX010000506         4415 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_770474, whole genome shotgun sequence.
ACCESSION   DASUEX010000506 DASUEX010000000
VERSION     DASUEX010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4415
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_770474"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1089
                     /gene="purF"
                     /locus_tag="VFV61_08485"
     CDS             <1..1089
                     /gene="purF"
                     /locus_tag="VFV61_08485"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEX4900554.1"
                     /translation="GNLPFAREERRKLEKEGAIFSATSDTEVILHGVARSRAQGVREA
                     IAEVLRNTEGAFSMLFLTPHELIAVRDARGFRPLALGRLGDAWVIASETCAFDLIDAR
                     YVRDVEPGEMIVIDEDGLRSSHPLKPQQHSMCLFEHVYFARPDSLIYGKSVNDSRHRM
                     GQRLAIEQPADADIVVPVPDSGTVAAIGYSAQSEISFRFGLVRNHYVGRTFIEPKQSI
                     RSFGVRIKLNPVRELIEGKRVVLIDDSIVRGTTSKKIVRMVREAGAKEIHLRISCPPT
                     ISPCYYGIDTPNKADLIAAQMSVAEICNFIEADSLGYLSLEGMIEATEIEANSACVAC
                     WNENYPTRITANAETMWEREHEASITEN"
     gene            1114..2256
                     /locus_tag="VFV61_08490"
     CDS             1114..2256
                     /locus_tag="VFV61_08490"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HEX4900555.1"
                     /translation="MGRKTKGCGCAAAVLVVILGAAGYAGWRYVLPWWRTKPPPPSGG
                     ELQVHILDVGPINGDSILIISPTGTTVLVDAGDTGKGKVVLEALQRYKVQQIDYFIAT
                     HPHPDHIGGADEVLKGTKVLNVLDNGLEPTLPASAAAQAKPTPAPGAKQQQGKTKPDK
                     RPASLKFFDEYQDAVLQSGARHEKAEVGKKLDLGGGARLTILGPGAPAFTKQQMKGGG
                     NEPNANSLVARLDYGSFSLLLPGDAEEQTEHRLLTKDLNLRADILKVSHHGSKYASAA
                     DYLERVKPKVAIISCGEWNRYGHPAQAVLDRFKAADIKLYRTDLQGEITITTRGREND
                     FAVKTAKEAKTDLWIGRMAQKDDSSRAGFIAYGDFGPPPKERKSNR"
     gene            2357..3391
                     /gene="purM"
                     /locus_tag="VFV61_08495"
     CDS             2357..3391
                     /gene="purM"
                     /locus_tag="VFV61_08495"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEX4900556.1"
                     /translation="MSKPVTYSDSGVDIDAANRATAKIKDLARRTFNERTLSEIGSFG
                     GMFDGAFPQLKQPVLVASADGVGTKLKIAFLTGVHDTIGRDLVNHCVNDILVQGARPL
                     FFLDYIATGKLSPDVVAGVVAGITEGCRENGCVLLGGETAEMPGFYADGEYDVAGFIV
                     GIVDRKKVIDGKQIAAGDVVLALPSVGLHTNGYSLARKLFLEVSGFTVDTKLESVGQT
                     VGEALLQPHLSYLTPLDGLLDSGSIKGLAHITGGGLTENIPRILPPDVAVEIKKGSWP
                     VLPLFTLMQEIGNVPEPEMFRTFNMGVGMVIICARQNAEAIKSHFKMRGQPCFSIGNV
                     TSGRQEVRFS"
     gene            3552..>4415
                     /locus_tag="VFV61_08500"
     CDS             3552..>4415
                     /locus_tag="VFV61_08500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900557.1"
                     /translation="MSASVDKDSITAVQASEEAKKKFLEELKRCEKETADKGRKFQKP
                     VSWLLGRQLIASLKSTLLYTAFGTKIDARDWMDAKVFPPARVVRNPGSADGANVAALL
                     TPEEEVAKAWEAIYKRDSPANFTGNSGEFWFDYISDTGDGMTATYSIAYLCLSDLWIR
                     DRYTEMPVKPTKEQIEQNQQPNELLVRMRTKKSDNPQGLENLVGLQKLPRGEFLLVGG
                     DTSYHMADYASLHLRFQQPFRWAYEDLREDRGLAPLKNPEEHLAEIKRFRPLFGIPGN
                     HDYYDMLDGFRR"
BASE COUNT         1063 a         1149 c         1239 g          964 t
ORIGIN      
        1 ggtaacctgc ccttcgcgcg cgaggagcgg cgcaagctgg aaaaagaagg cgcaattttc
       61 tctgcgacgt cagacaccga agtgattctg catggcgtgg cgcgttcgcg cgcccagggc
      121 gtgcgcgaag caattgcaga agtcttacgt aacacggagg gcgccttttc gatgttgttt
      181 cttacgccgc acgaattaat tgcagttcgt gatgcgcgag gtttccggcc cctcgctctc
      241 gggcggctcg gcgatgcctg ggtgatcgct tcggaaacgt gcgcgtttga tttgatcgac
      301 gcccgctatg tacgcgatgt ggagccgggc gaaatgattg tgatcgatga agacgggttg
      361 cgctcttcgc atcccctgaa gccgcaacaa cactcgatgt gtctctttga gcacgtttac
      421 tttgcgcggc ctgactccct gatttacggc aagtcagtca acgattctcg ccatcgcatg
      481 ggccagcgtc tggccataga gcaaccggcg gatgcggaca tcgtggtgcc cgtgccagac
      541 tcgggtactg tcgcagccat tggttattcc gcgcagagcg agattagttt tcgcttcggt
      601 ttggtgcgca accactatgt cggccggacc ttcatcgaac cgaagcagtc aatccgttcc
      661 ttcggagttc gcatcaaact aaatccggtg cgcgagctga ttgagggcaa gcgtgtggtg
      721 ctgattgatg attccattgt gcgcggcaca acttctaaaa agatcgtgag gatggttcgc
      781 gaagccggcg cgaaagaaat acatttgcgc attagttgcc cgcccacgat cagtccttgc
      841 tattacggca ttgatacacc caacaaagcg gatttgattg ccgcgcagat gtcggtggcg
      901 gaaatttgca atttcatcga agcggattcg ctgggctacc tttctttgga aggaatgatc
      961 gaagcgactg aaatcgaggc caattccgcg tgtgtggctt gctggaacga aaactatccg
     1021 acgcgaatca ccgccaacgc cgaaacgatg tgggaacggg aacacgaggc gtcaatcacc
     1081 gaaaactgag cgaccttaac gaggcgaatc ttagtgggcc ggaagaccaa aggttgtggc
     1141 tgcgctgccg ctgtgctggt agtaatttta ggcgcggccg gctatgcagg ttggcgttac
     1201 gtacttccct ggtggagaac caagccgccg ccgccatcag gcggcgagct tcaggtccac
     1261 attctcgatg tcggtccaat caacggtgat tcgatcctaa ttatttcgcc caccggtacg
     1321 actgtcctgg tagacgccgg cgacaccggc aaaggaaaag ttgtcctcga ggcgttgcag
     1381 cgttacaagg tgcaacaaat cgattacttc atcgccaccc acccgcaccc ggatcacatc
     1441 ggaggcgccg acgaagtgct caagggcacg aaagtcctca atgtcctcga caacggtctc
     1501 gaacctacgc tgccggcgag tgctgcggcc caagcgaaac cgactcccgc acctggggcg
     1561 aaacaacaac agggcaagac gaaacctgat aagcggccag cgagtcttaa gttttttgat
     1621 gaatatcaag acgccgtgtt acagagtggc gctcgtcatg agaaggcaga ggtggggaag
     1681 aaattggatc tgggtggcgg cgcgcgtttg acgatcctcg gtccaggggc gccagctttt
     1741 actaaacaac agatgaaagg tggcggcaac gaaccaaatg cgaattcgtt ggtggcccgc
     1801 cttgattatg gaagcttttc gcttttgctg cctggcgatg cggaagaaca gacagagcat
     1861 cgtctgttga cgaaggattt gaacctgcgc gccgacattt tgaaagtctc gcatcatggc
     1921 tcgaaatacg cctcagcagc tgactatctg gaaagggtga agccgaaggt ggctattatc
     1981 tcgtgtggcg agtggaatcg ttatggacac ccggcacagg cggttttgga tcgtttcaag
     2041 gcggccgaca taaaacttta tcgcacggac ttgcaaggtg agattacgat cacgacgagg
     2101 ggacgagaga acgacttcgc agttaagacc gctaaggaag caaagactga cttatggatt
     2161 gggcgcatgg cgcagaagga tgattcctcg cgcgccggtt tcatcgccta tggagatttc
     2221 ggcccgccac ccaaggaacg caaatcaaat cgctaaaagg tcccaagtct catgtctcaa
     2281 gtccgcgacc gtaacttgaa atcaaacttg cgacatgcga gatgagacct gaaacctgaa
     2341 acctgaaacc cattcaatga gcaaaccagt tacgtacagc gattccggag tcgacatcga
     2401 tgccgccaac cgggcgactg cgaagatcaa agatctcgca cgccggactt ttaacgaacg
     2461 cacgctttct gagatcggca gtttcggggg aatgtttgac ggcgccttcc cgcaactcaa
     2521 acagccggtc ctcgtagctt cggcagatgg cgtgggcaca aaactcaaga ttgcgtttct
     2581 gaccggagtg cacgatacca tcggccgcga tctcgtcaac cactgcgtta acgatatcct
     2641 cgttcagggt gcacgtcccc ttttctttct ggactacatc gctaccggaa agctttcgcc
     2701 cgacgtcgtt gccggcgtgg ttgcgggcat cacggaaggc tgccgtgaaa atggttgcgt
     2761 cttactcggc ggcgagactg ccgagatgcc gggcttctac gctgacggcg aatacgacgt
     2821 ggccggcttc atcgttggca tcgttgatcg caagaaggtc atcgacggga aacagattgc
     2881 agcgggagat gtggtgctgg ccctgccttc ggttggcctg cacacgaacg gttattcact
     2941 cgcccgcaag ctctttctcg aagtctcggg ttttacagta gacacaaagc tcgaatcagt
     3001 cggtcagaca gtaggcgagg ccctgttgca gcctcacctc agttacttga ctccccttga
     3061 tggcttactc gattcaggtt cgatcaaagg cctggcgcat attaccggcg gtgggttaac
     3121 ggagaatatt cctcgtattc tgccgccaga tgtcgcagta gagatcaaga aaggtagttg
     3181 gccggtgctc ccgctcttca cgctgatgca ggagatcggc aatgtacccg agccggagat
     3241 gtttcgaacc tttaacatgg gggtgggaat ggttataatc tgcgcccgtc agaacgccga
     3301 agcaataaag tctcacttca agatgcgcgg tcagccgtgc ttctcaattg gcaacgttac
     3361 ttctggtcgg caggaagtca ggttttcata accggatgac caggttctaa gggccgccca
     3421 acagttggtc ggtgaccaaa cagtcaggct caaacggagc taaattcaat cccgtctcgt
     3481 ttcgaccgtt cccgtgtcga acctcgcgct tttcaattcc cgacctcgtc gacttctaaa
     3541 ggaggaacct catgtcagct tcagtcgata aggattcaat cacagctgtt caagcgtcag
     3601 aggaagcaaa gaaaaagttc cttgaagagt taaaacgttg tgaaaaggaa actgcggaca
     3661 aaggcagaaa gttccaaaag ccggtgtcgt ggctcctggg acgtcagctt atagccagtc
     3721 tcaaatccac gctcctctac accgcgttcg gaacaaagat cgacgcccgc gattggatgg
     3781 acgcaaaggt gtttccgcct gcaagagtcg ttagaaatcc cgggagtgcg gacggtgcga
     3841 atgtagctgc acttttgact cccgaggaag aggtagcgaa ggcatgggaa gcaatttaca
     3901 agcgggatag tcccgcaaac tttaccggta atagtggtga gttttggttt gactacattt
     3961 ctgacaccgg agacggcatg actgccactt acagcattgc ctacctttgt ctcagcgatc
     4021 tctggattag agatcggtac acagaaatgc cggtcaaacc aacaaaggaa caaatagagc
     4081 agaaccaaca acccaacgaa ttgctggtaa ggatgcgaac gaaaaagtcc gacaatccac
     4141 aagggttgga gaatttagtt ggtttgcaaa aacttccacg cggagagttt cttttagtcg
     4201 gtggcgatac cagctaccac atggccgact acgccagttt gcatcttcga tttcaacaac
     4261 ctttcagatg ggcttacgaa gacttgaggg aagacagagg cctcgcaccg ctgaagaatc
     4321 cggaggaaca cttagcggaa atcaaacgct ttcgtccact cttcggaatt cccggtaacc
     4381 acgactacta cgacatgctg gatgggtttc ggcgg
//
LOCUS       DASUEX010000507         2199 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_899932, whole genome shotgun sequence.
ACCESSION   DASUEX010000507 DASUEX010000000
VERSION     DASUEX010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_899932"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2188)
                     /locus_tag="VFV61_08505"
     CDS             complement(<1..2188)
                     /locus_tag="VFV61_08505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX4900558.1"
                     /translation="MYVKFPLRSDPELIDPALAGRKVFVLIWTTTPWTLPANLGIAVH
                     PDFEYSAFAHGDEVFIVASELLDSVAEKCGLTATDAEPLQVLARFPGARLDRLECGHA
                     WLDRSSLLMVGDHVTLGGEADAEVELDVREARDAKKTGKSGTGCVHTAPGHGHDDFVL
                     GKSYGLEIYCPVDNSGHFTPEVEHFAGQSVFAANPKIVEFMRERGVLLFTETYEHRYP
                     HCWRCKNPVIFRATPQWFISMDRAGGPVVERDEDGRDRSNFTDNFSDASSSGANSLRA
                     AALAEIERVQWLPAWGLDRMRNMFKGRPDWCVSRQRVWGVSIPVFYCAKCNEAVADPQ
                     IINRVADVFEKESADAWYTRTARELLPEGYKCGNCGEQEWIKETDILDVWFDSGSSSI
                     AVLEHRPELRWPADVYIEGGDQFRGWFNSSLMIGLAVHNRAPYRTVLTHGWTVDAQGK
                     AMHKSAGNAVPPDEVIKESGAEILRLWCASSNYFDDMRCSAEILQRVTDGYRKLRNTA
                     RFALGNLHGFDPERDAVAETDLLEIDRWALAELEVVTRKVRQAYDAYEFHAVYHALYS
                     FCTVTLSARYFDIIKDRLYTFAPRSQARRSAQTALYRIADALARLLAPILAFTADEIW
                     ENLPAGGEVRPVSVHLATFPEAHQEANEDRFAKWERLFEIREDVLRALEEARIAKDIG
                     SSLEARVEVSATGATFDLLEHYRADLRYLFIVSQVDVLRAAAGTNEV"
BASE COUNT          488 a          666 c          608 g          437 t
ORIGIN      
        1 tgacctcatt ggttccggcc gccgcgcgta ggacatcgac ctgggaaacg ataaataggt
       61 aacgcaggtc ggcgcggtaa tgttctagca gatcaaacgt ggcgccggtc gccgagactt
      121 ccacgcgcgc ctccagggaa ctaccaatgt ccttggcgat gcgcgcttct tccaaggcac
      181 gcagtacatc ttcgcggatt tcaaacagcc gttcccattt cgcaaagcgg tcttcatttg
      241 cctcctggtg agcttcggga aaggtcgcga gatgaactga caccggccgc acttcgccac
      301 cagccggcaa gttttcccaa atttcgtcgg cggtgaaagc caggatcggt gcgagtaacc
      361 gtgccagagc atcggcaatt ctgtagagcg ccgtttgtgc tgagcgccgg gcctggcttc
      421 gtggtgcaaa cgtgtagaga cgatccttga tgatgtcaaa gtaacgcgcc gacaaagtaa
      481 ccgtgcaaaa actgtaaagc gcatgataga cggcgtgaaa ctcgtaagcg tcatacgcct
      541 ggcgcacttt ccgagtcact acctcaagct cggccaaagc ccagcgatcg atctcaagca
      601 ggtccgtttc cgccacggcg tcgcgttcgg gatcgaaccc atgcaggttg cccagcgcga
      661 aacgggcagt gttgcgcagc ttgcgatagc catcggtcac gcgttgcagg atttctgccg
      721 aacagcgcat gtcgtcgaag tagtttgaag acgcgcacca cagccggaga atctcagcgc
      781 ctgattcttt gatgacctca tcaggcggca ctgcgttgcc ggcagacttg tgcatcgctt
      841 tgccttgcgc atcgaccgtc caaccatgtg tcagaaccgt cctgtagggc gctcgattgt
      901 ggacagctag cccgatcatt aaggacgagt taaaccaacc gcgaaactgg tcgccgccct
      961 cgatgtaaac gtctgcgggc cagcgcagct cgggccggtg ctccaacaca gcgattgaag
     1021 acgagccgct gtcgaaccag acatcgagaa tgtcagtctc cttgatccac tcctgctccc
     1081 cacagttgcc gcacttatag ccttccggta gcagctctct tgcggtgcgg gtgtaccagg
     1141 catccgccga ctctttctca aacacatcgg caacccgatt aatgatctgc ggatcagcga
     1201 ccgcttcatt acatttcgca caatagaaaa caggaattga aacaccccaa acgcgctgcc
     1261 gggaaacgca ccagtccggc cggcctttga acatgttgcg catgcgatcc aggccccagg
     1321 cgggcagcca ttgcacgcgc tcgatttctg caagcgcagc cgcgcgcaac gaattagcgc
     1381 cggaagaact cgcgtcggaa aaattatcgg tgaaattgct gcggtcacga ccgtcttcgt
     1441 cgcgttccac caccggtcca cccgcccgat ccatggagat gaaccattgc ggcgtggcgc
     1501 gaaagataac tggattcttg cagcgccagc agtgcggata gcggtgttcg taggtttcgg
     1561 tgaagagcaa tacgccgcgc tcgcgcatga actcaacaat cttcgggttc gccgcaaaca
     1621 cgctctgacc cgcgaaatgt tcaacttcag gagtgaagtg tccagagtta tctacgggac
     1681 aatagatttc cagaccgtaa ctcttaccaa gcacaaagtc gtcgtgcccg tgaccgggcg
     1741 ccgtgtgtac gcaacccgtt cccgacttgc ccgtcttctt cgcatcgcgc gcctcgcgta
     1801 cgtcgagttc tacctccgca tcggcttcgc ctccgagcgt tacgtgatca cccaccatca
     1861 acagtgagga acggtccagc cacgcgtgcc cacactccag gcggtcaagc ctggcaccgg
     1921 gaaagcgagc cagcacttga agtggttcgg cgtcagtggc ggtcagtcca catttctcgg
     1981 caaccgaatc cagcagttca ctggcgacaa taaaaacctc atcgccatgt gcaaacgcgg
     2041 agtactcgaa atctgggtgg accgcgattc ccaggtttgc cgggagggtc cagggcgtgg
     2101 tcgtccagat caaaacaaag actttgcgtc cggcgagcgc tggatcgatc agctccggat
     2161 cgctgcgcaa ggggaatttg acatacaccg agggactgg
//
LOCUS       DASUEX010000508         3135 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1135061, whole genome shotgun sequence.
ACCESSION   DASUEX010000508 DASUEX010000000
VERSION     DASUEX010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3135
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1135061"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..643
                     /locus_tag="VFV61_08510"
     CDS             <1..643
                     /locus_tag="VFV61_08510"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX4900559.1"
                     /translation="AMVVLDAYQSAGTVPFNVKELGVDFATGGSVKWLCGGPGAGYLY
                     VRPDLHTTLQPKTTGWMAHEEPFAFATELRYAPNVARFLHGSPAIPALYAAQSGYRII
                     NEIGVENIRAKSVRQTQRLIALAEEAGFKVTSPKESSQRGGTITVMHEHAAAMTTELI
                     KREFIVDYRPGAGVRISPHFYTKDEELELIIEEMKSIRDSRAYAQHEVAGAAF"
     gene            747..1622
                     /locus_tag="VFV61_08515"
     CDS             747..1622
                     /locus_tag="VFV61_08515"
                     /inference="COORDINATES: protein motif:HMM:NF015272.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HEX4900560.1"
                     /translation="MMSQEYGADSLLSYNKYLRVPDLINLQDCLSGPEHHDELLFITV
                     HQAYELWFKQILHELDAALAMMRSDRVATAARTMARVVEIEKLLVTQIHILESMTPIN
                     FLGFRDKLNPASGFQSMQFREIEFTSGQKLESTLHEFRDDAFALERLQKRFSGPTLSD
                     EFFALLQRRGFNAPAEDKSLSETEQKKNYGQRTQAVLEILTHFEERYEEFQLAEALLE
                     HDEYFSLWRSHHIKMVERMVGAKRGTGGSEGIGYLRTTLNKKFFPELWEARTYLDVKH
                     GAQGCPFSSSSVDHQ"
     gene            1643..1951
                     /locus_tag="VFV61_08520"
     CDS             1643..1951
                     /locus_tag="VFV61_08520"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX4900561.1"
                     /translation="MTRERRKAPRLQVDFPVRWEGVLTQQVGTVSSISESGCFILTGG
                     QVQQKELIRLEIDLPSGSACFWSEVVDEAYEIGFAVRFTSADDEDMALLQRTLKDGLA
                     "
     gene            1965..>3135
                     /locus_tag="VFV61_08525"
     CDS             1965..>3135
                     /locus_tag="VFV61_08525"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX4900562.1"
                     /translation="MNIEFPEKFNLGHYFLYHNLDAGRENKTCLYFEDQSFTYGEACR
                     MSNRVGNALRELGVELEDRILIALPDCPEFVWTWFGAARIGAVITMVNPLLPAADYPY
                     YLEYTRARVAIIHESLVAALVESSRTTKHLRAIIVVGDAPAAEAAPRRRSGPAWVSFN
                     DVVMSAPEECTQADTHRDDIAIWLFTSGSTGHPKGAVHLQHDLPFNTEVFAKRTIGVA
                     ETDLTLAVPKLFFGYATGTNLLFPFAVGAATALFSERSTPEKIYSAIERYRPTILTTV
                     PTMINAMLNANTGADLSCLRFCYSAGEALPVELYERWQMTFGIEIYDGIGSAEMFHIY
                     ISNRPGDVKPGSLGRLVAGYDARIVGPDGAEVATGEMGTLRVRGDSAALCYWNAHE"
BASE COUNT          719 a          856 c          857 g          703 t
ORIGIN      
        1 ggcgatggtg gtgcttgacg cataccagtc cgcaggcacg gtccctttca acgtgaaaga
       61 gctgggagta gatttcgcaa ccggtggatc ggtgaagtgg ttgtgcggcg gtccgggagc
      121 cgggtacctg tacgtgcggc ctgatctgca cacgacgttg cagccaaaga ccaccggatg
      181 gatggctcat gaagagccgt tcgcgtttgc gaccgagctg cgctatgcgc ctaacgtcgc
      241 tcgattccta catggctcgc cggcaatccc agccctgtac gccgcgcagt ccggttatcg
      301 catcatcaat gagatcggag tggagaacat tcgggctaag agcgtcagac aaacacagcg
      361 gttgattgct ttagctgagg aagcgggttt taaagtgacg agtccgaaag agtcgtcgca
      421 acgtggcggt acgatcactg taatgcatga gcatgccgca gcaatgacga cggaattaat
      481 caagcgcgaa ttcatcgttg actatcgacc gggcgcggga gtgcgcatct caccccattt
      541 ctacaccaag gatgaagagt tggaactcat catcgaagag atgaagtcga tcagagattc
      601 gcgcgcctat gcgcaacatg aggtggcggg agcggcattc tagaaaaacg ttcttagagc
      661 tttgatctta gttcttcaaa gctcggtgtt tgaaagacac tctcaaagtt caaagttcaa
      721 agttcaaagc tcaaagctca aagatcatga tgtcccaaga atacggagct gattccctgc
      781 tctcttacaa caagtacctg cgcgtcccag atctaatcaa cttgcaggat tgcttgtctg
      841 gccctgagca tcacgatgaa ctgcttttca ttacggtcca ccaggcctat gaactttggt
      901 tcaaacaaat cctccatgag ctggatgcgg cgctggcaat gatgcgttcc gaccgcgttg
      961 caaccgccgc tcggaccatg gcgcgcgtgg tcgagatcga gaagttgctc gtcactcaga
     1021 tccacatcct ggagagcatg acgccgatca actttctcgg gttccgcgac aagctaaacc
     1081 ccgccagcgg atttcagtcg atgcagtttc gcgaaatcga gtttacttcc ggacaaaaac
     1141 ttgaaagcac cttgcatgag tttcgcgacg atgcgttcgc tcttgaacgg ctgcaaaaac
     1201 gtttttccgg ccccacgcta agcgacgaat tcttcgcctt gttgcaacgc cgcggtttta
     1261 atgctccggc ggaggataag tcactttctg agacggaaca gaaaaagaat tacggtcaac
     1321 gaactcaggc ggtgctggaa atactcaccc actttgaaga gcgttatgag gagtttcaac
     1381 tcgcggaagc cttacttgaa catgatgaat acttttcgct ctggcgctct catcacatca
     1441 aaatggtgga gcgaatggtc ggtgcgaaac gaggtaccgg cggctctgaa ggtatcggct
     1501 acctgcgcac tacgctgaac aaaaaattct ttccggaact gtgggaagca cgcacctacc
     1561 tggacgtcaa acacggggcc cagggctgtc ccttttccag ttcctcagtc gaccaccaat
     1621 gattcgccga gcctaaacct gcatgactag agagagacgt aaagcgccgc gcttgcaagt
     1681 agactttccc gtccgctggg aaggagtact gacgcaacag gtcggcacgg tttccagcat
     1741 tagcgagagt ggctgtttca tccttaccgg aggtcaggta cagcagaaag aactaatccg
     1801 cctggaaata gaccttcctt ccggctctgc gtgtttctgg tcggaggttg tcgatgaagc
     1861 gtacgagatt ggatttgcag tgcgtttcac atccgccgac gatgaagaca tggcgttgct
     1921 gcaacgcact ctgaaggacg gtttagcctg acgatttcaa caggatgaac atcgagttcc
     1981 ccgaaaagtt taatctcggc cactacttcc tctatcacaa tctggatgcg ggccgcgaaa
     2041 acaagacttg cctgtatttc gaggaccagt cttttactta cggtgaagcc tgccgcatgt
     2101 cgaatcgcgt cggcaacgcg ctgcgtgagt tgggcgtgga gcttgaagat cgcattctga
     2161 ttgcgttgcc tgactgtccg gagtttgtct ggacctggtt tggtgcggcg cgcatcggcg
     2221 cggtaatcac gatggtgaat ccgctcctgc ccgctgccga ctacccttac tacctggaat
     2281 acacgcgcgc ccgcgtggcc atcattcacg aatcattggt cgcagctctt gttgagagca
     2341 gccgcaccac aaaacacttg cgcgccatta ttgtggttgg cgacgcgcct gccgctgaag
     2401 cagcgccacg gcggcgcagt ggacccgcat gggtttcatt taacgacgtc gtaatgtcgg
     2461 cgcccgaaga gtgcacccaa gctgatactc atcgcgacga catcgccatc tggttgttca
     2521 cttccggctc caccggccat cctaaaggag ccgtgcattt gcaacacgac ctgcctttca
     2581 acacggaggt ctttgccaag cgcacgattg gagtcgccga aactgatctc accctcgcgg
     2641 tgcctaaact ttttttcggt tatgcgacag gaacaaatct cctctttccc ttcgcggtcg
     2701 gagccgcaac cgcactcttc tccgaacgct cgactccaga aaagatctac tccgccattg
     2761 aacgttaccg tcctactatc ctcaccaccg tgccgacgat gataaacgca atgctcaacg
     2821 cgaacaccgg agctgatctg tcatgcctcc gattctgcta ctccgccggg gaagcgctgc
     2881 ccgtggaact atatgagcgg tggcagatga cttttgggat cgaaatctat gatggcatcg
     2941 gttccgcgga gatgtttcac atctacatct cgaaccggcc aggagatgtt aagcccggaa
     3001 gtttgggtcg cctggtcgcg ggttatgacg caaggatcgt cgggccggac ggcgctgagg
     3061 tagcaacggg cgagatggga accttgcgcg tgcggggcga ttcagccgcg ctgtgctact
     3121 ggaatgcgca cgaag
//
LOCUS       DASUEX010000509         4870 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1468147, whole genome shotgun sequence.
ACCESSION   DASUEX010000509 DASUEX010000000
VERSION     DASUEX010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4870
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1468147"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..737
                     /locus_tag="VFV61_08530"
     CDS             <1..737
                     /locus_tag="VFV61_08530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900563.1"
                     /translation="SKCQTCGTLYMGPPLGHVRDPNKQSRFVEAELSALLQAAGGTLP
                     VIKKDDRGVYVLVEAAAGHAPAKPAAAPAKAAPTAAAKVATAAPKPDAAPKLVAKAGA
                     EPNRQLFKRSTYAGVKDTGPVAGESFAEKVARLKAVSAAVKQRADEGGGGHAVEAVEA
                     PAPSAPIEAAAEAPAPSAPAPETAAAPSSAAAHKPTGQADRSLMKRSTYCGVVDTGPI
                     PGETFAQKVERLAKLSAAVKQRADGG"
     gene            complement(748..1755)
                     /locus_tag="VFV61_08535"
     CDS             complement(748..1755)
                     /locus_tag="VFV61_08535"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900564.1"
                     /translation="MIIESSAPTRVDLAGGTIDIWPLYLFHPGAATVNFAISLRAHCR
                     IETRNDNRIILESSDRQLGFETSVSAVGELANEERLELISKLVHFYQPTGGFHLTTTS
                     QAPAGAGLGGSSALAIACIGALNRLQGNRYDERRFITLVANIETTVIRVPAGFQDYYP
                     AFYGSTSCIHFRPDGIEREALNINEAALEDRFVICYTGEPRLSGTNNWDILKRHIDGD
                     RELFALFEGIRDSAINMRAALLNDDWEGVQTIMSAAYPNRKRLAPGVTTPQMESLVAK
                     ALANGALAAKVCGAGGGGCIAFLCAPGSKQDVENALTAEEGAEVLSWKVAREGLTVRE
                     G"
     gene            complement(1919..2653)
                     /locus_tag="VFV61_08540"
     CDS             complement(1919..2653)
                     /locus_tag="VFV61_08540"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEX4900565.1"
                     /translation="MQLSFLYDLIGQYGLYGVMILVMIEGDITLLIAGVLAHSHFFGE
                     YSFARVLLWGTIGGVASDNIAYATGRGFRKGVSDLRFYRSASPRMERLTTKFGGLSIF
                     LSKYIYGLRWATCIFYGVSRMPFLRFLFLSVASCFLWVFVLSGAGYFFSGAVMGLIGD
                     FQRLGKVLLVIVIVGIVGFYLVERFWLSQRVERANPELLQEFEQVAQEKLHDLKQEFQ
                     EHIPFTHARRDEKKKRAETKRESDAD"
     gene            complement(2724..3191)
                     /locus_tag="VFV61_08545"
     CDS             complement(2724..3191)
                     /locus_tag="VFV61_08545"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX4900566.1"
                     /translation="MQIRSILFPTDFSECGNYALSYAASLARKFGAAIICVHVIEQIV
                     PTVGYTGMTEPLPLADITDQLEDSAERELPKIAECEECAGLEVDELIVHGDAAAEIVR
                     VAKERNVDLILISSHGRTGLGRILFGSTAEAVVRHASCPVLVVKPPQEEEAAN"
     gene            3396..4346
                     /locus_tag="VFV61_08550"
     CDS             3396..4346
                     /locus_tag="VFV61_08550"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEX4900567.1"
                     /translation="MNNPSGSAASFLGIDLSGPTMRAALVTATGEVIERRESPIEADK
                     LVPKVAEVVKELRSAHPEIAAVGIGIPGLVNRQTDRVIASRDLPSTVREELHSEFMDA
                     TGLRVEIENDANAAAYGEYIVGAGRGSRDMFYVTIGDGIGGAIILDGKLWTGASGFAG
                     EVGHITIDTDGIECECGNMGCLETVASGPNIVRRANERLHRDGTSSLSRLAINKNFTA
                     ADLAHEANNGDDFSLMMIERTGKYIGTGVASVLNLLNIEKIVLGGGVMDAGALILNPI
                     IQEAKRRAFQPCFEGTQIVAASLGADAVSIGAAMLARDAA"
     gene            4681..>4870
                     /locus_tag="VFV61_08555"
     CDS             4681..>4870
                     /locus_tag="VFV61_08555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900568.1"
                     /translation="MPQIFHHSTNALAKLSIFGAVFILLAALWVGAEINRSSWNTGQW
                     VERQQPIQFSHKHHVGDDG"
BASE COUNT         1162 a         1359 c         1307 g         1042 t
ORIGIN      
        1 tttcgaaatg tcagacctgc ggcacgcttt acatgggtcc gccgctggga cacgtgcgtg
       61 atcctaataa gcaaagccgg tttgtcgaag ccgagctgtc ggccttgctg caagccgcag
      121 gtggaacgct gcctgtaatc aagaaggatg accgcggcgt ttatgtgctg gtcgaagctg
      181 ctgcggggca cgcgccagca aagccggctg cggcgccagc taaagcagca cccactgcgg
      241 ccgcaaaagt tgcgaccgca gcgccgaagc ctgacgctgc gccaaagcta gtggcaaaag
      301 ctggggcaga accaaaccgg caattgttca aacgatccac gtacgccgga gttaaggaca
      361 ccggaccggt agcaggcgaa agttttgcgg agaaggttgc gcggttgaag gcagtgtcgg
      421 cggcggtaaa acaacgcgcg gacgaaggcg gcggaggtca cgctgttgaa gccgttgaag
      481 cgccggcccc atctgcaccg atcgaggcag cggccgaggc accggctcca agtgcaccag
      541 cgcccgagac agcagccgcg ccatcatctg cagcggcaca caaaccgacc ggtcaggcag
      601 atcgaagttt gatgaagcgt tcgacttatt gcggggttgt ggatacaggc ccgattcccg
      661 gtgagacttt cgcgcagaag gtggagcgcc tcgcgaaact ttccgccgcc gtcaaacaac
      721 gggctgatgg cgggtaagaa gaaagcctta accctcgcgt accgttagac cttcacgcgc
      781 aactttccag gacaaaactt cggcgccctc ctcggcagtg agcgcattct ccacgtcctg
      841 cttacttccc ggagcacata agaaagctat gcaaccgccg cctccggcgc cacagacctt
      901 cgcggcgagc gcgccattgg ccagggcctt cgcaaccaac gattccattt gcggcgtggt
      961 cacacccggt gcgagtctct tgcggttcgg ataggccgcg ctcatgatcg tttggacacc
     1021 ttcccaatcg tcattcaaca aggccgcgcg catattgatg gccgaatcgc gaatcccttc
     1081 aaacaacgca aagagctccc gatcgccatc gatgtgacgc ttcaggatgt cccagttgtt
     1141 tgtacccgag agccgtggtt cccccgtgta acagatcaca aagcgatctt ccagtgcagc
     1201 ttcgttaatg ttaagtgcct cacgctcgat cccatcgggc cgaaagtgaa tgcaggaagt
     1261 cgatccgtaa aacgccggat agtaatcctg gaatcctgca ggtacgcgaa tcacggttgt
     1321 ttcaatgttt gcaaccagcg tgatgaagcg acgctcgtcg taacgatttc cctgcaaccg
     1381 attcaaggca ccgatgcagg cgatggccag cgccgaagag ccgccgagtc cggcccccgc
     1441 cggcgcctgg ctggtagttg tcagatggaa gccgccggtg ggctgataaa aatggacgag
     1501 ctttgaaatc agttccagcc gttcttcgtt tgccaactca ccgacggcgg agacgctggt
     1561 ttcaaaaccg agctggcggt cgctcgattc cagaatgata cggttatcgt tgcgagtctc
     1621 aatacgacaa tgggcgcgta aggagatggc aaagttcacc gtggcggccc cgggatgaaa
     1681 gagatagagg ggccagatat cgatggtacc cccggccaag tcaacacggg tcggagcgga
     1741 agattcaata atcatctaag tgttttaaag aggtcccgtt tcttttaggt tgcacaactg
     1801 tactttgttc ttcgtgcttt gtactttgct ctcgcttatc tcggccgatt ccattgcgct
     1861 ggtcgtttca agttaagaag aacatcaaag cactaagtac aaagttcaaa gttccatttt
     1921 aatcggcatc cgattcgcgt tttgtctctg cgcgtttctt cttttcatca cgacgagcat
     1981 gcgtaaaggg aatatgttcc tgaaactcct gtttcagatc gtgtaacttt tcctgggcaa
     2041 cttgttcaaa ctcctgaagc aactccggat tggcgcgctc cactctttgc gacaaccaga
     2101 agcgctcgac cagataaaac ccgacgattc ccactatcac aatcaccagc agcacttttc
     2161 ccagacgctg gaaatcgccg atcaacccca tcaccgcgcc actgaaaaag tagccggcgc
     2221 cactcaagac aaacacccag agaaaacaac tggcaacgga aagaaaaagg aaacgtaaga
     2281 acggcatgcg gctaaccccg tagaaaatgc aggtcgccca acgcaagcca taaatgtatt
     2341 tggagagaaa gattgaaagc ccgccaaact ttgtcgtcaa ccgctccatg cgcgggctgg
     2401 cgctacggta gaaccggaga tcgcttacgc ccttacgaaa accgcggccg gtggcatagg
     2461 cgatgttgtc actggcaaca cccccgatcg tgccccacag caggactcgc gcaaaactat
     2521 actctccaaa aaaatggctg tgggccaaca caccggcgat taagagagta atatctccct
     2581 caatcattac caggatcata acgccgtaga gaccgtattg gccgatgagg tcgtagagaa
     2641 aactgagttg catgaaagcg tcagtagaca gttatcagtg gtcagccgcc agcgttctcg
     2701 ttctcgctga ccaccgccac tgatcagttt gctgcttctt cctcttgcgg gggcttcacc
     2761 accagcacgg gacacgaggc atgacgcacc accgcttcgg cggttgagcc gaaaagaatt
     2821 cggcccagtc cggttctccc gtgcgaggag atcagtatga gatccacgtt tcgctctttg
     2881 gcgacgcgta ctatctcagc cgctgcgtcg ccgtgaacga tcaactcatc aacctcgagc
     2941 ccggcacact cttcgcactc ggcaatcttc ggcagttcgc gctccgccga atcctcgagt
     3001 tgatccgtaa tatcagccag gggaagaggc tcggtcatcc ccgtatatcc cacagtcgga
     3061 acaatctgct ctatgacatg cacacaaata atagctgcgc cgaactttcg cgccagcgat
     3121 gcggcgtatg aaagtgcata gttaccgcat tcgctgaaat ccgtcggaaa cagaatcgaa
     3181 cgaatttgca tttgcagaga gaactttggg ccggggatag gatactttga accaccgcga
     3241 tctttggtgc aaagcgcaaa aaccggaaac gcaaaatcaa aaccctttct aaaacctagc
     3301 cattctacac gcgaagacac tggtttgaca gccctagtac cctcgactat tcttcgcctt
     3361 gtttaagttc tcgtgattac caaaccataa aaatcatgaa caatccgagc ggatctgcgg
     3421 caagctttct gggcatcgac ctctcaggtc ccaccatgcg ggcggcgttg gttaccgcca
     3481 ccggcgaagt aatcgaacga cgcgagagcc cgattgaggc cgacaaactg gttccgaaag
     3541 ttgcggaggt ggtgaaggag ctgcgcagcg cgcatcccga gattgccgcg gtcggcatcg
     3601 ggattcccgg ccttgttaac cggcaaaccg atcgggtcat tgcttcgcgc gatctgcctt
     3661 cgactgttcg tgaagaactc cattccgaat tcatggacgc tacgggattg cgcgtcgaga
     3721 ttgagaacga cgctaatgcc gcagcctatg gggagtacat tgtgggcgct ggtcgcggca
     3781 gtcgcgacat gttctacgtg acaatcggag acggtattgg cggtgcgatc attcttgatg
     3841 gcaagttatg gacaggtgcg tcggggtttg ctggcgaagt gggtcacatc acgatcgaca
     3901 cggacggaat tgagtgtgag tgcggaaata tgggatgcct ggaaacggta gcttccggcc
     3961 ccaacatcgt gcgccgcgcg aatgaacgct tgcatcgcga cggcacttcc tcgctttcgc
     4021 ggctggcgat taataaaaac ttcacagcgg cggatcttgc tcacgaggcg aataacggtg
     4081 acgatttttc gctgatgatg atcgagcgca ccggcaaata cattggcacc ggcgtcgcca
     4141 gtgtcctcaa cctgctgaac atcgagaaga tcgttctggg tggcggtgtt atggatgccg
     4201 gggctctgat tctcaatccg attattcagg aagcaaaacg acgcgccttc caaccctgct
     4261 ttgaaggcac acaaatcgtg gcagcatctc ttggtgccga cgcagtctca atcggcgccg
     4321 caatgttagc gcgagatgct gcttaactcc tcacgtctat ttgcatcttt aaagatcccg
     4381 ctttgagctt tgttcttcga attataccct gagtttctcg gactactggc agaagcgaac
     4441 aaagctcaaa gttctaagta caaagatcta acccgcaaaa tttgaaaaat tttggtcatc
     4501 tggtaaagtg ctctgacttt gcaagatgac aatttgtcct aatgttctgc tgacctgctg
     4561 cgtccttggg taggtccgca gcgcgattcg ataagtcgat ctgcgcgtag cttgctcttt
     4621 gggtcttccc gacgcgcgct ggtcgttgta gtcgcaaata ggattagcaa acctgcggcg
     4681 atgccacaga tctttcatca cagcaccaac gcgcttgcca agctaagcat ctttggcgcc
     4741 gtttttattt tgcttgctgc cctttgggta ggtgctgaaa tcaatcgttc atcctggaat
     4801 acgggccagt gggtcgaacg acaacaacca atccagttca gccataagca ccacgttggc
     4861 gacgacggca
//
LOCUS       DASUEX010000510         3197 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1226878, whole genome shotgun sequence.
ACCESSION   DASUEX010000510 DASUEX010000000
VERSION     DASUEX010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3197
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1226878"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="VFV61_08560"
     CDS             complement(<1..345)
                     /locus_tag="VFV61_08560"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEX4900569.1"
                     /translation="MIRNRRVACLFALSLIIGFAGAVRAQVTTGTISGSVADPNGAVV
                     QGANVMATNLETNASRSTTSDSDGHFAFTLLPPGRYRVDVTSQGFQDYRSEAVVNITQ
                     TTTLDVHLTLTGT"
     gene            complement(513..2045)
                     /locus_tag="VFV61_08565"
     CDS             complement(513..2045)
                     /locus_tag="VFV61_08565"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX4900570.1"
                     /translation="MNIGSLLPRHAQYRPHHTALIFEDRRLTFLELNRRVNRLANALL
                     SLGVKKGDKVATILPNSLELLECYWAIVKIGAVIVPLSQLLRGKGLLNLLRDSDTSTV
                     ITNSCIAGELESIKSELKDIAAERYISTDDNEGYQNYSALTAAASSDEPPKVEIKDDD
                     LFNIVYSSGTTGLPKGIVHTHYVRGMYCTMFASSFRMRPESVVIHAGSIVFNGAFVTL
                     MPALYLGATYVLQKHFEPEGFIAAVERERATHVMMVPSQIIAMLNSPAFSAEKLRSLE
                     AICSVGAPLHREHKDRLTATLPGCFYELYGLTEGFITILDKNDYAAKSDSVGVPIPFS
                     EMRIVDSLGREAAVGEVGEIVGYSPALMPGYYEQPELTAQAVVDGWLHSGDLGYVDED
                     GFLYLVDRKKDLIISGGVNVFPKDIEEIIVQHPAVREVAVFGVPSEKWGETPLAAVIL
                     QRPGEVTEDELCDWINERVDGKHQRVRAVVFMQDFPRSTAGKTLKREMREPYWAGRNV
                     RI"
     gene            complement(2042..3085)
                     /locus_tag="VFV61_08570"
     CDS             complement(2042..3085)
                     /locus_tag="VFV61_08570"
                     /inference="COORDINATES: protein motif:HMM:NF021982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HEX4900571.1"
                     /translation="MKVKQLIISVIALAFLHTAAYGGSWVSGSVKNSFGSRDYKLWVS
                     PGYRKENAIPLVLMLHGCMQKAEDLAAASGMNALADENNFLVVYPEQIPTANPLSCWN
                     WFDPKHQGRDAGEPALIAAVIEDLRSSYNVDAKRIYVVGISAGGAMAVVMAASYPELF
                     AGLGVIAGEEYKAATTVEGGLASMKTGGPDPNQQGLLAYQAMQKSLAGAKKRMPVIAF
                     HGKKDPYLKPLNTDQLIAQWAQTNDYLDDGKDNDSVSVQSPNETKGAVPDGYSYTRGQ
                     YKDSNGRLLMEKWIVEGLGHAWPGSPTANQFADPKGPNASAEIWRFFGETNLDSSVSQ
                     GTSPTVKPKGGDE"
BASE COUNT          703 a          910 c          817 g          767 t
ORIGIN      
        1 agtgccggtc agggtaaggt gaacatcaag cgtcgtggtc tgggtgatat tcacgaccgc
       61 ctcgctccga taatcctgaa agccctgaga ggtaacgtcg acgcgatagc gccccggcgg
      121 caataacgta aaagcaaaat ggccgtcgct atccgaagtt gtcgaccgag acgcgttcgt
      181 ttcaagattc gtcgccatta cgttcgcacc ctggacgaca gcgccgttgg gatctgccac
      241 cgagccgctg attgttccag ttgtcacttg cgctcttacg gcgccggcga atccgataat
      301 caaactgagc gcgaacagac acgctactct tctatttcta atcattgttt ggacctccag
      361 aaacattcaa gagcttcaca tcatgaatcc gccactgcat accaaagtct cgccactgat
      421 gtagttcgac tctggcaggc agagcaggtg aattcggcgc cggaccgttt aactagttca
      481 atcgtctcct gcactgggtc gcgtcattgc tcctagatcc gaacgttgcg gcctgcccag
      541 taaggttccc gcatctcgcg cttgagcgtt ttgcctgcgg tgctgcgagg aaagtcctgc
      601 ataaatacga ctgccctgac gcgctgatgt tttccgtcaa cgcgctcatt gatccagtcg
      661 cacaattcat cttcagtgac ttcgcctggt cgttgcagga tcaccgcagc gagcggtgtc
      721 tccccccatt tttcgctggg cacaccgaag accgccactt cgcgcaccgc gggatgctgc
      781 acgatgatct cttcgatgtc tttcggaaag acgtttactc cgccggagat aatcagatcc
      841 ttcttgcgat cgacgaggta aagaaaaccg tcctcatcca cgtagccgag gtctccactg
      901 tgcaaccaac catcgactac agcctgtgcg gtcagttccg gttgttcgta atagccgggc
      961 attagggcag ggctgtatcc cacgatttca ccaacttcgc cgacggcagc ttctcttccc
     1021 aggctatcga ctatacgcat ttcggaaaaa ggaatcggca cgcctaccga gtcagatttg
     1081 gctgcataat cgttcttgtc gaggatcgtg atgaatcctt cggtcaagcc gtagagttcg
     1141 taaaaacaac ccggcaatgt tgccgtcaat cgatctttgt gctctcgatg cagcggtgcg
     1201 ccaaccgagc aaatggcttc cagcgaacgc aacttctcgg ctgaaaaggc tggcgaattt
     1261 aacattgcga tgatttgcga tggcaccatc atcacgtgtg tcgcacgctc gcgctcaacc
     1321 gcagcgataa agccttccgg ttcgaaatgt ttttgcagga cgtaggttgc gccgagatac
     1381 aacgcgggca tcagcgttac aaacgcgcca ttgaagacga tcgagccggc gtgaatgaca
     1441 acgctttccg gacgcatacg aaaggaggag gcgaacatcg tgcagtacat tcccctgacg
     1501 taatgcgtat gcacaatgcc tttgggaagc ccggtcgtgc cgctgctgta gacgatgttg
     1561 aatagatcgt cgtctttgat ttctaccttc ggcggttcgt cgctgctggc tgccgccgtt
     1621 aacgcggagt agttttggta gccctcatta tcgtcggtcg agatatagcg ctcggccgcg
     1681 atgtctttaa gttcggactt gatggactcc aattctccag cgatgcagga gtttgtgatt
     1741 actgtcgaag tgtcggaatc tcgcagcagg tttagcagcc ctttgccacg cagcaattga
     1801 ctgagcggga caatcaccgc gccgatcttt acgatggccc agtagcattc aagcagctca
     1861 aggctattcg gtaagatcgt tgctactttg tcgcccttct tcacgcccag gctcagcagc
     1921 gcgttggcaa gccgattgac acgccggtta agttccagaa aggtcaaccg acgatcttca
     1981 aatatgagcg cggtatgatg gggacggtat tgcgcatgcc gggggagaag agaaccgata
     2041 ttcattcgtc gccccctttc ggcttgacgg tcggcgatgt accctgcgag accgaagaat
     2101 ccaaattagt ttcaccgaaa aatctccaga tctccgcact cgcattcggt cctttcgggt
     2161 cggcaaattg atttgcagtc ggactccccg gccaggcatg accgagtcct tccacgatcc
     2221 atttctccat taacaatcgt ccattactgt ctttgtactg ccccctcgtg tagctatagc
     2281 cgtcgggaac agcacctttt gtttcgttgg gtgattgaac gctaacgctg tcgttgtctt
     2341 taccatcgtc caggtaatcg tttgtctgcg cccattgagc aattagctgg tcagtgttaa
     2401 gcggtttcag atacggatcc ttctttccat gaaaagcgat tacaggcatt cgcttttttg
     2461 caccagccaa actcttctgc attgcctggt aagcaagcag gccctgttgg ttcggatcgg
     2521 gaccacccgt cttcatcgag gccaagcccc cctcaacggt tgtggcggct ttgtattcct
     2581 ctccggcgat cactccgagg ccggcgaaga gctccggata gctcgccgcc atcactacgg
     2641 ccatcgcgcc acctgcggat atgccgacta catagatacg cttggcatcg acgttatatg
     2701 aagaccgcag atcctcaatg acggcggcga tgagagcagg ctcacccgcg tcgcgccctt
     2761 ggtgctttgg gtcgaaccaa ttccaacaac tcaggggatt ggccgtcggt atctgctcgg
     2821 gataaacaac cagaaaatta ttctcatctg caagagcatt catccccgac gcagcggcaa
     2881 gatcttcagc cttctgcatg cagccgtgca gcatcaacac gaggggaatc gcgttctctt
     2941 tacggtatcc gggcgacacc cacaatttgt aatcacgcga gccaaaagaa ttcttaaccg
     3001 acccgctcac ccaactgcct ccgtaggctg cagtgtgcaa aaaggcgagc gcgataaccg
     3061 agatgattag ttgtttgact ttcatggctt ctcgcaagtt tcggcaaatt cgacgatcaa
     3121 atcagctacc ctcgaagcac cgcgttcacc aagcgtgatg gtgaagtgcg ttgtgcctga
     3181 gatcacctcg tccttca
//
LOCUS       DASUEX010000511         3446 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_651944, whole genome shotgun sequence.
ACCESSION   DASUEX010000511 DASUEX010000000
VERSION     DASUEX010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3446
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_651944"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1856
                     /locus_tag="VFV61_08575"
     CDS             <1..1856
                     /locus_tag="VFV61_08575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900572.1"
                     /translation="VSWHDSPKIEVDGKKVPDPARAEFDPVSNRHYLISAVTLGPVTM
                     SVQQVDGSEKTQLVFPLVVKPCPRWETEQVRSALDRIASATEALRGHYRLYAYTDARI
                     GQDPSKDGFVMLETMRPDENIRCAGQSRTTATLSTVGMVCSTFIWLATERANKIGAAN
                     NPPLPHIILDGRPRRIHRPEAELLGCQSFFRRYPLSDEIPPGTPDGLYFYTEDERRRA
                     AKWLFERLKSDIKKEVTTSADLQAKVDALMAESGTLWSLWSILKLLATFSVPQVAILL
                     NVAPSVIEKLVTFLTDMPEDVANQMGNAFANDDCYWTAKDKDTWRKQPGSGTTVSPDD
                     IINFWAPPTHEDQNVVHGLYGYNQRIRLLPPSFEKNAPPPSIWKLSQGVGDMGGRVTF
                     RGLGVPNARIRVGCGKYSSGADGYYGLGHVPEGLYWAEASYFHPQTKLHLKAKGRPVQ
                     VPLFGSAYVDFELEEPPDTRREVIVAAHMDLVNRYAIGEDWWGHPECEPEVVYLGLDM
                     FPPNNPAFDEMRNKSLRGKVGKSEQVDDWGQAQFECDLEIQPDRSIKFVCRARLKEDE
                     DDDWQHEDTFFVAPKILETDASYKHVFDLVRSEDAWPVRAHIVLTIDNNRL"
     gene            1902..2399
                     /locus_tag="VFV61_08580"
     CDS             1902..2399
                     /locus_tag="VFV61_08580"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX4900573.1"
                     /translation="MEIELRRTTNKDLGFVLAAESDEENRPFITAWPEEKHRAVLDDA
                     NIEHLIIESRPNNQRVGFVILAGLQNEHRNIEFMRLVVTEKGKGYGRAAVQAIKRHAF
                     ERLAAHRLWLDVKERNTRARVLYEKEGFRYEGTLRECLKGPDGFESLVVMSVLEQEMQ
                     KYRNA"
     gene            2392..2769
                     /locus_tag="VFV61_08585"
     CDS             2392..2769
                     /locus_tag="VFV61_08585"
                     /inference="COORDINATES: protein motif:HMM:NF021525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2255 family protein"
                     /protein_id="HEX4900574.1"
                     /translation="MPDQLKFTEDVVEVLRNAKILGVRAGTEHRYTGVWVVVVNGRVF
                     VRSWSDKPTGWFRAFRKHPSGMIQAAKLEIPVRARFTRSEPMRHAVTAAFGEKYNTKG
                     SRQWVEGFAEPEREATTLEFIPR"
     gene            2813..>3446
                     /locus_tag="VFV61_08590"
     CDS             2813..>3446
                     /locus_tag="VFV61_08590"
                     /inference="COORDINATES: protein motif:HMM:NF015402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtpA/SgcQ family protein"
                     /protein_id="HEX4900575.1"
                     /translation="MDRLGTIKSIFGVSRALIGVVHLQALPGTPANKLDVAAIASVAV
                     DEARAYETAGFHGVMIENTHDRPYLKSAVGPEITAAMAVVAAEIRKSIALPLGIQVLA
                     GANSAAVAVALAAGASFVRVEGFIFAHVADEGLIEASAGALLRYRRAMGADHIRVFAD
                     IKKKHSAHAITADVDIVETAKAAEFFSTDGVVVSGPATGRPADPEEVQAVS"
BASE COUNT          804 a          919 c         1004 g          719 t
ORIGIN      
        1 acgtttcctg gcacgacagt cccaagatcg aggtggacgg gaaaaaagtt ccggatccgg
       61 cgagggcaga gttcgaccca gtgagcaaca gacattacct aatcagtgct gtcacgcttg
      121 ggccagtgac aatgtcagtg caacaagtcg acggtagtga gaaaacgcag ctggtttttc
      181 ctctcgtcgt gaagccctgc ccacgttggg aaacggagca agtccgtagc gctctggacc
      241 gtattgcgag tgcgacagag gcgttgcgcg ggcactatcg cctttacgct tacaccgatg
      301 ctcgcatcgg acaggatcct tcgaaggatg gattcgtaat gctggagacc atgcgtcccg
      361 atgaaaacat ccgctgcgcc ggacagagta gaacgaccgc gactctttct acggtgggta
      421 tggtctgttc aacgttcatc tggctcgcca ccgagcgagc gaacaaaatt ggcgctgcaa
      481 acaatccgcc actcccgcac atcattcttg atggccgtcc gcggcgtatc catcgtcctg
      541 aggcggagtt gctcggctgc cagtcttttt ttcgcagata tcctctgagt gatgagatcc
      601 cgcccggcac gcccgacggt ttgtacttct acacggagga tgaacggcgc cgcgcggcca
      661 agtggttatt tgaaagattg aagagcgaca tcaagaaaga ggtgaccacc tcagcagacc
      721 tacaagccaa agtggacgcc ctcatggccg agtcaggaac actctggtcg ctttggtcga
      781 ttcttaaact gctagcgacg ttctctgtac cgcaggtagc catcctcctc aatgttgcac
      841 cttcagtgat agagaagctg gtaacttttc tgacagacat gccggaggac gtcgccaacc
      901 agatgggtaa cgcgttcgcc aatgacgact gctattggac cgccaaggat aaagacacct
      961 ggcgtaagca acccggcagc ggtactacgg tgagccccga cgatatcatc aacttctggg
     1021 caccgccgac ccatgaggat cagaacgtag ttcacggtct ttatggctac aaccagcgca
     1081 tccggttatt gccgccgagc ttcgagaaga atgccccgcc tccaagcatc tggaaactct
     1141 ctcaaggggt cggcgatatg ggaggacggg tgacgtttcg tgggctgggc gttcccaacg
     1201 cccgcattcg tgtcggctgc ggcaagtact cctccggcgc tgacggttac tacggactgg
     1261 gccatgttcc agagggtctc tactgggccg aagcctctta tttccatccc caaaccaaat
     1321 tacatctcaa agccaagggt cggccggtcc aggtgccgct gtttggttcc gcctatgtcg
     1381 acttcgaact ggaagaacct cctgacacgc gccgcgaggt gattgtcgcc gctcatatgg
     1441 acctcgtaaa ccgctatgca atcggcgaag attggtgggg ccacccagaa tgcgaacccg
     1501 aggttgttta ccttggcctc gacatgttcc ccccgaacaa tcccgctttc gacgagatgc
     1561 gcaataagag cctgcggggc aaggtgggga agagtgaaca ggtagatgat tgggggcagg
     1621 cgcagttcga atgcgacctg gagatccagc ccgaccgatc catcaaattc gtgtgccgcg
     1681 cccgtctcaa ggaagacgaa gacgatgatt ggcagcatga ggataccttc ttcgtcgcgc
     1741 cgaagattct cgaaacagat gccagttaca aacacgtgtt cgacctggtt cgttcggaag
     1801 acgcctggcc tgtgcgcgcg cacattgtct tgaccatcga caataatcgt ctctagattc
     1861 ccatctgata gtcaccggtt atttgttgaa gaaaggggct catggaaatc gagctacggc
     1921 gaacgactaa caaagatctc ggctttgtgc tggcagcgga gagtgatgag gagaatcggc
     1981 ctttcatcac cgcctggccg gaagaaaagc atcgcgcagt cctggacgac gcgaatattg
     2041 aacacttgat aattgaatcc agaccaaaca accaacgtgt tggttttgta attctggcag
     2101 gcttgcaaaa cgagcatcgc aacatcgaat tcatgcgcct cgtggtaact gagaaaggaa
     2161 aaggctacgg tagggcagcg gtgcaggcga tcaagcgaca tgcattcgag agattagcgg
     2221 ctcaccgcct ctggctcgac gtcaaagagc gcaacacgcg ggcaagagtg ttgtatgaaa
     2281 aggaaggttt tcgctatgaa ggcaccttga gggagtgtct taaagggccc gacggatttg
     2341 aatcgctggt tgtgatgtcc gtgctcgagc aagaaatgca aaagtaccgt aatgcctgac
     2401 cagcttaagt tcactgagga cgtcgtcgaa gtgctgcgga acgcgaagat tctcggcgta
     2461 cgagccggaa cggaacaccg ctacacgggt gtctgggttg tggtagttaa cggtagggta
     2521 tttgtgcgct cgtggagcga caaaccaaca ggatggtttc gcgcgttccg gaagcatccg
     2581 agcggaatga ttcaagcggc gaagctggag attcctgttc gcgccaggtt cactcgcagt
     2641 gaacctatgc gacatgcagt cacagctgct ttcggcgaaa agtacaacac caaaggatct
     2701 cggcagtggg tcgaaggatt cgctgaacca gaaagagaag cgactacgct tgagttcatt
     2761 ccccgctgac gatcgatagt cttagaacct attacatcgg agcgattatt ccgtggacag
     2821 actcggaaca atcaaatcta tctttggcgt cagtcgtgca ctgatcggcg ttgttcacct
     2881 acaggcgtta ccagggacgc cagcaaacaa actcgatgtt gctgccatag cgtcggtagc
     2941 cgtggacgaa gcgcgcgctt acgaaaccgc gggttttcat ggcgtgatga tcgaaaacac
     3001 gcacgatcgt ccttacttga agagtgctgt aggtccagaa ataaccgcgg cgatggctgt
     3061 cgttgccgcg gagatccgca agtcaatcgc gttgccgctc ggtatccagg tgcttgccgg
     3121 agccaactca gccgcggtgg cagttgcgct cgcagccgga gccagcttcg tgcgtgttga
     3181 aggctttatc tttgcgcatg ttgctgatga gggtttgatc gaagcgagcg cgggtgccct
     3241 gctgcgatat cgtcgcgcga tgggagctga tcacattcga gtcttcgccg acattaagaa
     3301 gaaacactcc gcgcacgcca tcaccgcgga cgtggacatc gtggagacgg cgaaagcggc
     3361 cgagttcttc tccacggacg gcgtcgtggt gagcggccct gcgaccgggc gtccggccga
     3421 tccggaggaa gtgcaagccg tgagca
//
LOCUS       DASUEX010000512         3143 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_771468, whole genome shotgun sequence.
ACCESSION   DASUEX010000512 DASUEX010000000
VERSION     DASUEX010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3143
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_771468"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            368..1174
                     /locus_tag="VFV61_08595"
     CDS             368..1174
                     /locus_tag="VFV61_08595"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX4900576.1"
                     /translation="MTEAKMQESVEYKPGTFCWVELGTTDGEAAKKFYTQLFDWEFDD
                     HDMGPGGVYTILKQNGKDVGALYTMPEEMTANGVPPHWMSYISVANADEATAKAKSLG
                     ANVMKEPFDVFTMGRMAVIQDPTGAMFALWQPGTHSGAGVVNTPNSLCWNELATKDAA
                     RAGEFYSGVFGWEKNVQQMGPMTYTSFMNGDRPAGGMYEPGPEMGEVPPHWMIYFAVA
                     DTDATLARATELGGKSCVPPMDVPGVGRMAIMTDPQGAAFAFIKLLNAPA"
     gene            1299..2213
                     /locus_tag="VFV61_08600"
     CDS             1299..2213
                     /locus_tag="VFV61_08600"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX4900577.1"
                     /translation="MKPRLVWLLLCCIWGSTWLFIKLGLEDLPPFTFAGIRFVIAVAI
                     LAAIVALRRLSLPKTPRDWLLLVISGILAFCVNYGLLFWGEQYISSGLAAVLQSTIPA
                     FGLVIAHFYLPGEKMTPLKILGVVLGVIGVAVIFSNQLAVAGPRALAGCAALVVSSIG
                     AAYSNVLVKTYGKHLDPAILAGGQMFFGLIPLLLIGIPWEGSPLNFHWTPTALVALFY
                     LAIVGSVVAFLLYYWLVQNMDVTKTMLIALVTPVVAVTLGMLVLNEELHRRTLLGGLM
                     IISGIAIIVTRQAPKVPSAKSQVQSPLA"
     gene            complement(2249..2587)
                     /locus_tag="VFV61_08605"
     CDS             complement(2249..2587)
                     /locus_tag="VFV61_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900578.1"
                     /translation="MSSEDLPNELETRNAKLEAEFAGFALTECLIQTDPQTEKYLRRV
                     IRTAFELAVYNRFGAEIGGFWLMTEQIEQLRRLPDYYSPNEAVFDAHAVEIYLQAQLH
                     NSYCALQDRS"
     gene            complement(2643..>3143)
                     /gene="lexA"
                     /locus_tag="VFV61_08610"
     CDS             complement(2643..>3143)
                     /gene="lexA"
                     /locus_tag="VFV61_08610"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEX4900579.1"
                     /translation="SVHSILMALEREGLIKRIPNVSRGIELIDQETATDTYEIPLLGT
                     VAAGQPIEAILTHDTVGVPRDMVGQGRTFALRVRGDSMIEENIQDGDTIIVSAQRTAQ
                     NGQMVVALIDGNYATVKKFYREPEFIRLEPANPQFKPIFIKTPERIEIQGIVRGLIRR
                     YATPAA"
BASE COUNT          698 a          802 c          871 g          772 t
ORIGIN      
        1 cgtaaccgaa ggtggaggaa aagccaccac aacaaaaacc taacggcacc acatccgcaa
       61 ggatgtgagg ttggtgaaag ccaatcgcgt gctgaccggt gaaacactta ccgcaagtct
      121 tcatcgggat acaaatcggc aacttcagcg tgtacatgcg gagttgccac cctatagaaa
      181 gcttcggcga tttgtaggaa cggattgcgc tactgtcagc ttgctgagag tagcgctttt
      241 cgtttttctg gcgtacattt tccccgaatt agtcatccta acaatccaac aggcgaaatc
      301 taatcagtgc tggaaccggc tcgatctgcg cgtacccggg cactgagaaa ttccgaagga
      361 gaaaaaaatg accgaagcta aaatgcaaga gagtgttgag tacaagccgg ggacgttttg
      421 ctgggtagag ttgggaacca ccgacggtga agctgcgaag aagttttaca cgcagttgtt
      481 tgactgggag tttgacgatc acgatatggg cccaggcggc gtctacacga ttctcaaaca
      541 gaacggcaaa gacgtgggcg cgctctatac aatgccggaa gagatgacgg cgaatggtgt
      601 tccaccgcac tggatgtctt acatctcagt cgctaacgct gacgaagcga ccgctaaagc
      661 gaaatcgtta ggcgcaaatg tgatgaaaga accctttgac gtatttacga tgggacggat
      721 ggcagtgatt caggatccga caggtgcgat gtttgcactt tggcaaccag gaacgcattc
      781 aggcgcagga gtcgtaaaca cgccgaattc tctctgttgg aacgagctag ccactaagga
      841 cgcggccagg gcgggcgagt tctacagcgg cgtgtttggc tgggagaaaa atgtccagca
      901 gatgggaccg atgacttaca cgtcattcat gaacggcgat cgcccggccg gaggaatgta
      961 cgagccgggt ccggaaatgg gtgaggtacc gccacactgg atgatctatt ttgcagttgc
     1021 cgatacggat gccacgctcg cccgcgccac cgaacttggt ggcaaatcgt gtgtgccacc
     1081 aatggacgta ccgggagtcg gtcgcatggc gatcatgacc gatccgcaag gtgcggcatt
     1141 cgcgtttatc aagctactta atgcgccggc gtaagtgaca tcgattctgt agtgcaggag
     1201 cggcaaagtt aggacgcagt cctaatgagc cgctcttgtt attaagttga tcctctttcg
     1261 cccttcctaa aacaaattcg aaccagttac tctgagtcat gaaaccacgc ctcgtctggc
     1321 ttctactctg ttgcatctgg ggctcgacct ggctcttcat caaactcgga ctcgaagacc
     1381 ttccaccctt cacattcgcc ggcatccgct ttgttattgc ggtcgccatc ctcgccgcca
     1441 ttgtcgcgct tcgccggttg tctttgccaa aaacgccgcg tgactggttg ctgctggtga
     1501 ttagcggcat cctggctttt tgtgtgaact atggattgtt gttctggggt gaacaataca
     1561 tctcgtcggg cttagctgct gttttgcaat ccacgatacc ggctttcggc ttggtaatcg
     1621 ctcacttcta tctgccgggc gaaaagatga cgcctctaaa gatacttggg gttgtcctgg
     1681 gagtgattgg ggtagcggtc atcttttcga atcaactggc tgttgccggt cccagggcgc
     1741 tcgccggatg cgcagcgctt gttgtcagct ccatcggtgc tgcctattca aatgtcctgg
     1801 tgaaaaccta tggcaagcat cttgatccgg cgattcttgc tggcgggcaa atgttcttcg
     1861 gcttaattcc tttgctgttg atcggaattc cgtgggaggg gagtccgttg aatttccatt
     1921 ggacgcccac ggcgctagtg gcattgttct accttgcgat cgttggctcg gtagtcgcgt
     1981 tcctgctgta ctactggctg gtgcaaaaca tggacgtgac caagacaatg ttaattgctc
     2041 tggttactcc agtagtcgcg gtgacgttgg gaatgctggt tctcaatgag gaactgcacc
     2101 ggcgcacgct cttaggtggg ctgatgatca tttccggaat tgcgatcatc gtgacaaggc
     2161 aggcgccgaa agtcccaagt gccaagtccc aggtccaaag tccgcttgcc taacgatcca
     2221 gtgaaacagg cattatttgg ttcgccgctc aagatctatc ttgtaaggcg caataagagt
     2281 tgtggagttg tgcctgcaag tagatctcga ccgcgtgggc gtcaaagaca gcctcatttg
     2341 gcgagtagta gtcaggcagt cttcgcagtt gttcaatctg ctcggtcatc agccagaatc
     2401 cgccgatctc tgctccaaag cgattgtaaa ctgccaactc gaatgcggtc cggattacgc
     2461 gccgcaaata cttttctgtt tgcgggtcgg tttggatcag acactccgtg agcgcaaagc
     2521 cggcaaactc tgcttccagt ttggcgtttc gcgtttcgag ttcgttgggt agatcttcag
     2581 aagacatttg tattttcccg aaaccccaaa cctgaaacct gaaacttgga acccgaaacc
     2641 tcttatgccg ccggtgtcgc gtatctccga atcaagccgc gcactatgcc ctggatctcg
     2701 atacgctcag gcgtcttgat gaagatcggt ttgaattgtg gattggcagg ctcgaggcgg
     2761 atgaactctg gctcgcggta aaacttcttc accgtcgcat aatttccatc aataagcgcc
     2821 accaccatct gtccgttttg cgctgtgcgc tgtgcggaga cgatgatggt gtcgccatcc
     2881 tgaatattct cctcaatcat tgaatcaccg cggacgcgta atgcgaaggt gcgtccctgt
     2941 cccaccatgt cgcgcggcac gccaacggtg tcgtgagtga gaatcgcttc gataggctga
     3001 ccagcggcca cggtgccgag caagggaatt tcgtaggtgt cggtggcggt ttcctgatcg
     3061 ataagttcga taccacgact aacgttgggg attcgtttga ttagtccttc gcgctccagg
     3121 gccatcagga tgctatgcac gct
//
LOCUS       DASUEX010000513         2776 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_652233, whole genome shotgun sequence.
ACCESSION   DASUEX010000513 DASUEX010000000
VERSION     DASUEX010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2776
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_652233"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /locus_tag="VFV61_08615"
     CDS             complement(<1..475)
                     /locus_tag="VFV61_08615"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEX4900580.1"
                     /translation="MPNLLDIRRRLKSVKNTQQITKAMKMVSAAKLKRAQDRVVTARP
                     FANKMIEVLSELAGRTDENFHHPLLDERGDQRYLVVVITADKGLCGAFNTNLIKAAQA
                     FVREHAGKTIEIVAVGRKGRDFFRRRGVLNGEYIGLTGKGRVDFAEAIGVARDVIN"
     gene            complement(505..2091)
                     /gene="atpA"
                     /locus_tag="VFV61_08620"
     CDS             complement(505..2091)
                     /gene="atpA"
                     /locus_tag="VFV61_08620"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEX4900581.1"
                     /translation="MDQLRANEINEIIRKQIQNFEVGVTVMEVGTVIKVGDGIAEIHG
                     LEKVMAGELLEFPHGVRGLALNLEEDKVGAVLFGEFQAIKEGDLVKRTGRIMQVPVGE
                     SLIGRVVDALGVPIDDQGPILTENFNPIERIAPGVVDRQPVKEPLQTGLKAIDAMVPI
                     GRGQRELIIGDRQTGKTAVAVDTIINQKGKDVICIYVAIGQKASTVAQVVKTLHDFGA
                     MEYTIVVKATASDPAAMQFLAPYSGAAMGEYFRDNGKHALTIYDDLSKQAAAYREISL
                     LLRRPPGREAYPGDVFYLHSRLLERAAKLSDAKGGGSLTSLPIIETQAGDISAYIPTN
                     VISITDGQIFLEADLFNSGIRPAINVGNSVSRVGGSAQIKAMKQVAGTLRIDLAQFRE
                     LQAFAQFGSDLDKATQAQLARGQRLVEILKQPQYRPMAIENQVLVIWAASNGFTDDIA
                     VEEVKGFETELLKFIENSHPGLLQNIREKKSLTDEIKSDLQVVLKDFKDLWKEKTATT
                     DASMSAAASTSAADPVTVSA"
     gene            complement(2167..2712)
                     /gene="atpH"
                     /locus_tag="VFV61_08625"
     CDS             complement(2167..2712)
                     /gene="atpH"
                     /locus_tag="VFV61_08625"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HEX4900582.1"
                     /translation="MSSQSVSRRYAVALADVLSGSGQERTIQDELNSWERMISSNPEL
                     RDAFANPTVPYEQKRGVLNELIAKTKVNAITANFLQILLKNQRLTALGEINSRLATVL
                     DDRAGAVAAHVTTANPVNEETKLILSEQLSRFTGKSVRLSFAVDDSLIGGIVTRVGST
                     VYDGSIRTQLDQMEKALAGKA"
BASE COUNT          596 a          809 c          666 g          705 t
ORIGIN      
        1 ggttgattac atctcgggct actccaatag cctccgcaaa atcgacgcgc cccttgccag
       61 taagcccgat gtattcccca ttcagtactc cgcggcggcg aaagaagtct cttcctttcc
      121 gtcccaccgc cactatctcg atggtctttc ccgcatgctc tcgaacaaag gcctgagcgg
      181 ctttgattag attcgtattg aacgctccgc aaagtccttt gtcagcagtg atcaccacga
      241 cgagatagcg ctgatcaccc cgttcatcca acaacgggtg atgaaaattc tcgtccgtcc
      301 tccctgccaa ctcactcagc acttctatca tcttgttggc gaaaggtcgc gcggtcacca
      361 cacgatcctg tgcgcgcttc agctttgccg ccgaaaccat cttcatggct ttggtaattt
      421 gctgggtgtt ctttaccgac ttaagccggc ggcggatgtc taacagattc ggcatcgtaa
      481 gttcgttaag cgaacaaatt aagattaggc acttacggtc acaggatcag cagcggatgt
      541 acttgcagcg gcgctcatcg aggcatccgt cgtcgcggtt ttttccttcc acagatcctt
      601 gaaatctttg agtaccactt gcagatccga cttgatctcg tcagtcagac tcttcttttc
      661 gcggatgttt tggagcaaac ccgggtgaga attctcgatg aacttgagca gttctgtttc
      721 gaacccctta acttcttcaa ctgcgatgtc atctgtaaac ccgttactcg cggcccagat
      781 tacaagcact tggttttcaa tcgccatcgg ccgatattgc ggctgtttca gaatttctac
      841 cagccgctgt ccgcgcgcaa gttgggcctg agtggccttg tcaagatcgg atccaaattg
      901 ggcaaacgcc tgtagttctc taaactgagc taggtcaatt cgcagcgtgc cggcaacttg
      961 tttcatcgcc ttgatctgcg ccgaaccgcc cacacgcgag acgctgttac ctacgttaat
     1021 cgctggacga atgccgctat tgaagagatc cgcttccaaa aagatctggc catccgtgat
     1081 cgagattacg ttagtcggaa tatacgcgga aatgtcaccg gcctgggtct caataatggg
     1141 caggcttgtc agcgaaccgc cgcccttagc atcagatagt tttgcagcac gctctaacaa
     1201 acgggagtgc agataaaaaa cgtcgccagg gtaggcctca cgtcccggtg gccgccggag
     1261 cagcaacgag atttctcgat aagctgccgc ttgttttgaa aggtcatcgt aaattgtcag
     1321 cgcatgtttg ccgttgtcgc ggaaatactc gcccatcgca gccccggaat acggagccag
     1381 gaattgcatc gccgccgggt cagaagccgt tgccttcaca acaatcgtgt actccatggc
     1441 tccgaagtcg tggagcgttt tgactacttg cgcgactgtc gaggcctttt gcccgatcgc
     1501 aacgtagatg caaattacat ccttaccctt ctggttaata atcgtatcga ccgcaaccgc
     1561 cgtcttgccg gtttgccggt cgccaatgat cagctcgcgc tgtccacggc cgatcggaac
     1621 catcgcgtcg atagccttca ggcctgtttg caacggttct ttgaccggtt gccgatcgac
     1681 cacaccaggg gcgatacgct caatcggatt aaagttttca gtcagaatcg gaccttgatc
     1741 gtctatcggc actcccagcg cgtcgaccac tctgccgata aggctctcgc cgacagggac
     1801 ttgcatgatt cgtccggtgc gcttaaccag gtcgccttcc ttgatcgcct ggaattcgcc
     1861 aaaaagaacg gcgccgacct tatcttcttc caagtttagc gccaggccgc gaacgccatg
     1921 ggggaactcc agtagttcgc ccgccatcac cttttccagt ccatgaatct cagcgatgcc
     1981 gtcgcccacc ttgatgaccg tccctacttc catcacggtg acgcctactt cgaaattttg
     2041 aatctgcttc cgaatgatct cattgatctc gttagcccta agttggtcca tttcaatcct
     2101 ctatgctttt cagtttctcc gtcgctttat tggcacggtc ctgattgcgc cgctagtgtc
     2161 aaagtttcaa gccttgcctg cgagtgcttt ttccatctgg tctaactgcg tacgaatcga
     2221 cccatcgtac acagtcgaac ccacccttgt gacgattccg ccaatcaagg aatcatcaac
     2281 ggcgaagctc agccggacag attttccggt gaaccgagaa agctgctccg ataagatcaa
     2341 tttggtttcc tcgttcacgg gattggcagt cgtgacatga gcggcgacag caccagctcg
     2401 atcatcaagc acggtggcga gcctggagtt tatctcgccc aaagcggtca gcctctgatt
     2461 ttttagaagg atctgcagga aatttgcagt gatcgcgtta accttggtct tagcgatgag
     2521 ctcgtttaga acccctctct tctgttcata ggggacagtg ggattcgcaa atgcgtctcg
     2581 cagttccggg ttcgaactga tcatccgctc ccaggagtta agctcgtctt gaatggttcg
     2641 ctcctgcccg ctgccactta acacatccgc aagagcaacc gcgtagcggc ggcttactga
     2701 ctgggaactc aatgtcgcac ccctccgaac tggtcggcag aaacctgtat tattcgcgca
     2761 tctttctcga catcaa
//
LOCUS       DASUEX010000514         2088 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1007309, whole genome shotgun sequence.
ACCESSION   DASUEX010000514 DASUEX010000000
VERSION     DASUEX010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1007309"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            50..>2088
                     /locus_tag="VFV61_08630"
     CDS             50..>2088
                     /locus_tag="VFV61_08630"
                     /inference="COORDINATES: protein motif:HMM:NF013776.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX4900583.1"
                     /translation="MQTSLSRSQLDLNRLYSALRQLAEGLHALHQTGKLHRDIKPSNV
                     LVTQEGRVVILDFGLVAEVEGRGIHDSVSLAGTPDYMSPEQGAQLPISSASDWYSIGV
                     MLYQALTGQLPFKGKFFEVMMSKQNLDPPAPAELVRDLPPELNDLSMRLLSRNPAQRP
                     TSREILRILGHGKTGQLRPALMPSAPSSVIETAPFVGRGKQLRELETAFNDVRIGDTV
                     TVFVHGSSGMGKTALVRHFLDELKEREHNLVVLEGRCYERESVPFKALDGVVDSLTKY
                     LVSLPEGRADVLMPRDVSTLARLFPVMLQIDSVFDAPQRAEEIPDPLTLRRRAFTALR
                     ELLARISDRQQLVIYIDDLQWSDADSTTLLEDLLRPPGSPALLLLASFRSEDVDTKPF
                     LRTLLESTNHKSRRELEVTALSKEEANKLVRAQLGSAANEIQPFVESMLREARGNPFL
                     LEQLARYAATTEQRGTTGITLAVMLDARLRQLPKGARHFVDALAIAGRPVNPEVAYQA
                     AGLSGDELPLVTSLRSAQFLRSGGSDHSVELYHDRIRETLAAQLDQAKVKQIHRRLAQ
                     AIEARGIDDPESLFEHYLGAGERVRAALHAGVAAKKAAASLAFDRAAAFYRRALELAP
                     VRGAELIDMKQSLADALVNAGRPAEAAEAYLEVSEYTSAARSLDFKRRAAEQLLMG"
BASE COUNT          450 a          588 c          605 g          445 t
ORIGIN      
        1 cgattcaatg gatatcgatt tttcgaatga ctcagtttcg gaaacatcat tgcaaacctc
       61 actttcgcgt tctcaacttg acctgaaccg tctctacagc gctttgcggc aactggcgga
      121 aggactacac gcactccatc aaacaggaaa actacaccgc gacatcaaac cttcaaatgt
      181 gctggtgacg caggaaggcc gcgtcgtgat tctcgacttt gggcttgtag ccgaagtcga
      241 gggacgtgga atacacgata gcgttagtct cgccgggacc cctgactaca tgtcgccaga
      301 acaaggtgct cagcttccta tttcaagtgc gagcgattgg tacagcatcg gggtgatgct
      361 ctatcaggcg cttactggtc aactcccttt caagggaaag ttttttgaag tcatgatgag
      421 caagcagaac ctggatccac ctgcacctgc ggaactggtt cgcgatctgc cgccagagct
      481 caacgacctc agcatgcggt tgcttagccg aaatccggcg cagcggccca ccagtcgaga
      541 aatcctgcgc atcttgggtc atggtaagac tggccaattg aggccggcgc tgatgccctc
      601 ggccccttct tcagttattg agacggcgcc gtttgtaggc aggggaaaac agctgcgcga
      661 gctggagaca gcgtttaatg atgttcgaat tggagatacg gtaacggttt tcgttcatgg
      721 cagctcaggc atgggcaaga ctgccctggt acgccacttc ctggatgagc tgaaggagcg
      781 cgaacacaat ctcgtggtac tggaaggccg ctgttatgag cgcgagtccg tcccctttaa
      841 ggcgctcgac ggcgtcgtcg atagtttgac gaagtatctc gtctcgctgc ctgagggtag
      901 agctgatgtg ttgatgccaa gagatgtgtc gacgctcgcc cggctctttc cggtgatgct
      961 gcagatcgat tccgtgtttg atgctccgca gcgtgcggaa gagataccgg atcctttgac
     1021 gctcaggcgc cgcgctttca ccgcgctgcg tgaacttctg gcccgaatct cggaccgcca
     1081 acagttggta atctacatcg acgacctgca atggtcagac gccgatagca caacactact
     1141 ggaagatctg ctgcgaccgc cgggctcgcc ggcgttgctc ttacttgcca gctttcgcag
     1201 cgaagacgtt gacacaaagc catttctccg cacgctcctg gaaagcacga atcacaagag
     1261 tcgcagagaa cttgaggtca cggcgctcag caaggaagaa gccaacaagc ttgtgcgcgc
     1321 ccagcttggg tcagccgcca atgagatcca accgtttgtc gagtcgatgt tgcgagaggc
     1381 gcgcgggaat ccatttctgc tggaacaact cgcacgctat gccgccacca ctgaacagag
     1441 agggacaacg ggaatcacgt tggccgtaat gctggatgcc aggctgcgtc agcttccgaa
     1501 aggtgcgcgc cactttgttg acgcccttgc catcgccggc cgtccagtca atccggaagt
     1561 tgcctatcag gcggcagggt tatccggcga tgaactcccg ctcgtcactt ctctcaggag
     1621 tgctcagttc ctgcgaagtg gcgggtcgga tcactcggtt gagctctatc acgatcgcat
     1681 tcgcgaaaca ttggccgcac aactcgatca ggcaaaagtg aaacagattc accgccgtct
     1741 ggcccaggca attgaagccc gtggaattga cgatcccgag tcgctattcg aacactacct
     1801 cggcgcgggt gaacgcgtcc gcgccgcatt gcatgccggc gtggcggcaa agaaggcggc
     1861 ggcttcactt gcctttgatc gtgcggctgc tttctaccgg cgggcgttgg aactggcgcc
     1921 ggtaagaggc gcggaactga tcgacatgaa gcagtcgctg gcggatgcgc tggtcaatgc
     1981 gggccgtccg gctgaagccg ccgaagcata tttagaggtt tcagagtaca cctctgccgc
     2041 ccgcagtctg gatttcaagc gacgcgccgc ggagcaacta ctgatggg
//
LOCUS       DASUEX010000515         2315 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_93421, whole genome shotgun sequence.
ACCESSION   DASUEX010000515 DASUEX010000000
VERSION     DASUEX010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93421"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="VFV61_08635"
     CDS             <1..430
                     /locus_tag="VFV61_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900584.1"
                     /translation="SALPFLKDALRLQLERFPSVRNGLNRIEGTGLALVSAGVTRFAD
                     LFPRFVAAEPSYGLGDAQLWNALRQLTLGRSPLLLDVSESGEMWEHVLAITDAGRAVL
                     SGSTDRVSLNGIDEWLGGVHLQGHANIWRWDEAQQRLVSV"
     gene            642..989
                     /locus_tag="VFV61_08640"
     CDS             642..989
                     /locus_tag="VFV61_08640"
                     /inference="COORDINATES: protein motif:HMM:NF016152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEX4900585.1"
                     /translation="MDIESVRKFCLSLPHVTEGIQWENDLLLRIGNKMFVVLSLEPES
                     DHVMSFKCTPEKFAELVERDGIKPAPYVARYHWVSLERFDALNKRELEPLLRTAYELV
                     RDKLPKKVRDGLK"
     gene            1216..>2315
                     /locus_tag="VFV61_08645"
     CDS             1216..>2315
                     /locus_tag="VFV61_08645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900586.1"
                     /translation="MRPKFSLPLLLFVALSFTAIVKAQNPLEPLARQAVSKDGKKAAA
                     AIAELRNAGPAGLEAMLAVHADAISQQVLEPLKPSTPEWERLSAALDAVSQQRNSYLS
                     GLYWYTDMGEAKAAAKRSGKPILSLRLLGNLSEEFSCANSRFFRTILYSNASISKKLR
                     EDFVLHWKSVRPVPHVTIDFGDGRKLERTLTGNSIHYILDVEGRPIEALPGLYGPNAF
                     LRALLVSENVFAKLREAKGPNPGGALVTYRRNRINRLSANWLADIQKSGGKMPVGLVQ
                     ARTPDGTPTAIVATGTVVTKAVTEVSLLRSLTFGAESLGRITDEETWRKIAQLHLADA
                     QLDSRSIGLIQRETQKVLGKNDLKSLLEKLQL"
BASE COUNT          543 a          621 c          650 g          501 t
ORIGIN      
        1 gagtgccttg ccctttctga aagacgcgtt gcgtctgcag cttgagcgtt ttccttcggt
       61 gcgcaacgga ttgaatagga tagagggcac gggactggcg ttggtgagcg cgggagttac
      121 acgctttgcg gatctcttcc cccggtttgt tgcggccgag ccgagttacg gtctgggcga
      181 cgcgcaattg tggaatgcgc tgcgacagtt gacgcttggg aggtctccat tgctgctcga
      241 tgtcagtgag agcggggaga tgtgggaaca cgttttggcg atcactgatg caggcagagc
      301 agtgttgagt ggtagtacgg atcgcgtgag tctgaatgga atcgacgagt ggttaggcgg
      361 ggtccactta cagggacatg caaacatctg gcgctgggat gaagctcagc agagacttgt
      421 gagcgtctga gtctcggatt cgatcttgct ttacagccaa gacgccgaga gcgccaagtg
      481 ggcaaagatg agaaaagaaa ccaggattgc gtcctaaata agctctgcat tgtgaggacg
      541 ttagctgaca accttctttg cgctccccac gggcggcctc taccgcaccg aaacctggaa
      601 cctggaaccc gaaacctgaa acctgtaacc cgaaaccctt tatggacatt gaatctgttc
      661 gcaaattttg tctttcatta cctcatgtca ccgaaggcat ccagtgggag aacgatctgt
      721 tgctgcgcat tggcaacaaa atgtttgttg tgctttcact cgaaccggag agtgaccacg
      781 tcatgtcgtt caagtgcacg cccgagaagt ttgccgaact cgtggagcgc gacggcatta
      841 agccggcgcc ttatgtcgct cgttatcatt gggtgtcgct cgagcgattt gacgcactaa
      901 acaaacgtga actggaaccg ctgctgcgaa cggcgtatga actcgttcgc gacaagttgc
      961 caaagaaggt tcgggacggg ctcaagtgaa agaaggagtt aagtaggtag tggtcagtgg
     1021 tcagtagtcc gtagtgaact cgatggttaa agcctaagcg ctaaaccacc agatagcctg
     1081 accacagaac aacagaccac ggacaactga ccctaaccac tggctacgga gcaaccctta
     1141 ctcgcctttc acgcctccaa aagccatccc tccccacccg ctcgactcaa tttgccaatc
     1201 cgcccggagg tccttatgag gcccaagttc tcgcttccgc ttcttctgtt tgtcgcactc
     1261 tccttcacag ccatcgtcaa agcccaaaac ccactcgaac cactcgcccg acaggctgtt
     1321 tcgaaagacg gcaagaaagc cgccgcggcc atagctgagt tgcgaaatgc cggcccggcc
     1381 ggactggagg cgatgcttgc cgtccatgcc gatgccatca gccaacaagt acttgagccc
     1441 ctaaaaccga gcacgcctga gtgggaacgt cttagcgccg cgctcgacgc ggtcagtcaa
     1501 cagaggaaca gttatctctc cgggctctac tggtacacgg atatgggcga ggccaaagcc
     1561 gccgccaaaa gatccggtaa gccgatcctc tcattgcgac tgctgggcaa cctcagcgaa
     1621 gagtttagct gcgcgaacag tcgcttcttc cgcaccatcc tttattccaa tgcttcaatc
     1681 tcgaaaaaat tgcgcgaaga ttttgtgttg cattggaagt cagtgcgacc cgttccacac
     1741 gtaacgatcg actttggcga tggacgaaag ttagagcgca ccttaaccgg caatagcatc
     1801 cattacattc tcgacgtgga gggtcgtccc atcgaggcct tgccgggtct ctacggaccg
     1861 aacgcgtttc tgcgtgcttt gctcgtgtcg gaaaatgtat ttgccaaact gcgcgaggcg
     1921 aagggaccca accccggcgg cgcgctagtc acttaccgcc ggaataggat taaccggctc
     1981 agtgcgaatt ggctcgctga cattcaaaag tctggcggca agatgccggt ggggttggtt
     2041 caagcacgta cgcctgacgg cactcccacc gcgattgtag ctaccggtac ggttgtcacg
     2101 aaggcagtca cggaggtgag tttgttacgc tcgctaacgt tcggtgctga gtcgttaggt
     2161 cgaatcaccg acgaagagac gtggcggaaa atcgcgcaat tgcatctggc ggatgcgcaa
     2221 ctggattcgc gcagcattgg tttgatccaa cgagaaacgc agaaggtact cgggaaaaat
     2281 gatctgaaga gtctcctgga aaaactccaa ttgaa
//
LOCUS       DASUEX010000516         2772 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_772543, whole genome shotgun sequence.
ACCESSION   DASUEX010000516 DASUEX010000000
VERSION     DASUEX010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_772543"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(292..912)
                     /locus_tag="VFV61_08650"
     CDS             complement(292..912)
                     /locus_tag="VFV61_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900587.1"
                     /translation="MSEAQVDDSAKVFEIELRKLEVELKRLEIEQKKLDPNYRKAEHR
                     AKNIDMIVKALSVLAVMIGVLVTYVQYSGTASLQRQQLLENEKNEIRAASRESLKPFN
                     EKRILLYTEASNVVAKLANLGEGEERQAARKRFFELYWGELALVEDKQVESAMVYFAR
                     ALQEYEQNPSSNAELQKQSLNVAHAFRESLKEGLDYPELGTLADKK"
     gene            complement(1077..1553)
                     /locus_tag="VFV61_08655"
     CDS             complement(1077..1553)
                     /locus_tag="VFV61_08655"
                     /inference="COORDINATES: protein motif:HMM:NF014373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEX4900588.1"
                     /translation="MEILWEELSRGLPNLRQVMVVIIRLFAATLLGALVGIQRERAGK
                     PAGLRTHILVALGTAVFVIACSAAGMSTDGLSRVVQGIITGIGFVGAGSILKLSEERD
                     IKGLTTAASVWMTAAIGVAVGFGSLGIALLSTLFTLITLALEGLYHSRRERTATTK"
     gene            complement(1642..>2772)
                     /locus_tag="VFV61_08660"
     CDS             complement(1642..>2772)
                     /locus_tag="VFV61_08660"
                     /inference="COORDINATES: protein motif:HMM:NF016103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HEX4900589.1"
                     /translation="IKLVIAALLVLRCFSAPHAQNDSPITAYLREHAETPVNYVMSKA
                     TSHRVTIIGEGHWLKQDVTLIAALIPLLQKADLDLAAEFFPAAEQARIDALITAAKWN
                     EQEANAIMRAAAWPYQEYRDLLRTAWSANQGAKRPIKILALSPPQDWRKVLLPRGETY
                     ESFMADLVSKHLKEARRNLVVYCGMHHAFTRYYQAELDKDARARAYMDRMGNILSRRL
                     GEQVFLITLHKPTWCGNLEPRSYCLPFGGRIDCEATEVGRPLGFDVVGSPWAEMRFDR
                     ADYYAYGHVALRFVDYTDGYIWSGPLESFRPVTIIPLSEYAPDSQTREQLTHENPFND
                     EVNVTAKRLEEIWAQQTAANQDLLSRRNWKNLVGWQARCPKP"
BASE COUNT          647 a          762 c          654 g          709 t
ORIGIN      
        1 acgatgaaat ccacaatgcc caacggaaac catagaatta atgccaaagg aagcaagtta
       61 ccgatcgccg acgtcattag atttagttca tgcacagcgc aatagttcca acttcagagc
      121 tactgttagg atgcccaact attaattatc tagcaagcgc tcaataacaa catctgccac
      181 ggtattatgc gacacgcgct tgataataac gctacacgga cattatcttg cgaccgcttt
      241 ctgatcctca caactggagc ctcacatcat gcctgcagca cacacactgc ttcatttttt
      301 gtcggccaga gtacctagtt ccggataatc caaaccttct ttcaatgatt ctctgaaggc
      361 gtgggcgacg ttgagcgact gcttttgcag ttcagcgttt gaggaaggat tctgctcgta
      421 ttcctggaga gctcgcgcaa aataaaccat tgccgactca acttgtttat cttccacgag
      481 ggcaagttca ccccagtaga gttcgaagaa gcgcttgcgt gctgcttgtc tttcctcgcc
      541 ttcacccaga tttgccagtt ttgcaaccac attcgaggct tccgtgtaga gcaggatccg
      601 tttttcatta aatggtttga gcgattcgcg tgaggcagca cggatttcat tcttttcgtt
      661 ttctaacagc tgctgtcgtt gcaggcttgc tgttcccgaa tattgaacat aagtcaccag
      721 gacacctatc atcaccgcta acacgcttaa ggctttgaca atcatatcga tgttcttggc
      781 acgatgctcg gccttccgat aattcggatc gagtttcttc tgctcgatct ctaatcgttt
      841 gagctcgact tccagtttcc tgagctctat ctcaaatact tttgcggagt cgtcaacttg
      901 tgcttcgctc acggcggttc cttttcagat tagtgaaaac atttagtcga gtggagagaa
      961 tcgattttag cggttcgtca agtttgatgt gcaacggtgc tcgagaatcg attccatttc
     1021 aaactacttg ttgccctgcc aatctgaaat tgtattttga tttcccctta gcagggctac
     1081 ttcgtcgtcg ctgttctttc ccgacgcgag tgatagagac cttctaaggc cagggtaatc
     1141 aacgtaaaca aagtgctcag caacgcaatg cccaaactac caaatcccac cgcaacgccg
     1201 atggccgccg tcatccaaac gctcgcggcg gtggtcagtc cttttatgtc gcgctcttca
     1261 cttagtttga gaatcgaacc agcgccgacg aagccgatgc cggtaatgat tccctgaact
     1321 acgcgggaca aaccatccgt agacattccg gctgcggagc aagctatcac gaagacggct
     1381 gttcccagtg cgaccaaaat atgtgttcgc aatcccgccg gcttgccggc ccgttcacgt
     1441 tgaattccca ccaacgctcc cagcagcgtg gcagcgaaca gccggatgat taccaccatc
     1501 acttgtctta agttcggcag gcccctgctc agttcttccc acaagatttc cattttgctt
     1561 ctctctcgga tcgccagcgt gattccaatc gggccagaag gatagtccta ttctctgtca
     1621 actcagggag gttccaggcc tttacggttt tggacagcgc gcttgccagc caacaagatt
     1681 cttccagttt ctgcgtgaca agagatcctg attggctgcc gtttgctgtg cccatatttc
     1741 ttccagcctt ttagccgtga cgtttacctc atcattgaag gggttctcat gagtcaattg
     1801 ttccctcgtt tgactgtccg gagcatattc acttagcgga atgattgtga caggacgaaa
     1861 cgattcaagg gggccagacc aaatatagcc gtcggtgtag tcaacaaagc gcagcgccac
     1921 atgaccgtag gcgtaataat cagccctgtc aaagcgcatc tcagcccaag gtgagcccac
     1981 aacatcgaat ccgagaggac gaccaacttc tgttgcctcg caatcaatcc gacccccgaa
     2041 tggtaaacaa taggaccgcg gctcgagatt gccacaccac gttggcttat gcagcgttat
     2101 gagaaacacc tgttctccga ggcggcgcga aagaatgtta cccattcgat ccatatacgc
     2161 ccgggccctc gcatctttat ccagttcagc ctggtagtag cgtgtaaatg cgtgatgcat
     2221 gccgcaataa acaaccaggt tccggcgagc ctcctttaga tgcttggaga ccaaatcggc
     2281 cataaatgat tcataagttt ccccgcgcgg cagcaaaacc ttgcgccagt cctgcggcgg
     2341 cgacaacgcg aggattttga tgggccgctt tgcaccctga tttgcagacc aggcagtccg
     2401 aagcagatcg cgatattcct gatagggcca ggctgccgcg cgcataattg cgttagcttc
     2461 ctgctcattc catttcgccg ccgtgatcaa cgcatcaatt cgcgcttgtt ccgctgccgg
     2521 aaagaactca gcggccagat ccaggtctgc tttctgaagg aggggtatca gtgcggctat
     2581 gagggtgacg tcctgcttca gccagtggcc ctcaccgata attgtcacgc ggtgggatgt
     2641 cgccttcgac ataacatagt tgacgggcgt ctctgcatgc tcccgtagat aagcagttat
     2701 tggggaatca ttttgcgcgt gcggggcgct gaaacatctc aggacaagga gcgccgctat
     2761 cacaagcttg at
//
LOCUS       DASUEX010000517         2214 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1678449, whole genome shotgun sequence.
ACCESSION   DASUEX010000517 DASUEX010000000
VERSION     DASUEX010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1678449"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..564
                     /gene="coaE"
                     /locus_tag="VFV61_08665"
     CDS             <1..564
                     /gene="coaE"
                     /locus_tag="VFV61_08665"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEX4900590.1"
                     /translation="REVVVPGSPGLAAVVETFGADVLASDGTLDRKRLGELVFRDEAK
                     RRLLNSILHPFIIERQDQQIRAWESEDPNGIAVVDAALMIESGGYSRFDKLIVVYCRP
                     EIQLQRIMKREGFSLEEAKRRIAAQMSQEEKLKFADFRIDTSGEFDQTRIQVEEVYRE
                     LQLLSGKQQLNPVASKRARFETPGKNR"
     gene            594..>2214
                     /locus_tag="VFV61_08670"
     CDS             594..>2214
                     /locus_tag="VFV61_08670"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX4900591.1"
                     /translation="MKRLSQCRSAVVAVLLLAMSLSHASCFSNEAGSPFYGRVLSPKL
                     QEFRWSDGGLPQIFDPAFAAAPPDTDLVRAIFEGLTDYDPTTLRPVPAVATRWESSKD
                     GKVWTFFLREDARWSNGELVTADDFIRSWQRTVRLGDQAPHTELLQNIEGASAGINPP
                     VAPARQQRSSNEKARSLPARRQQPFGAEAVGTHVLRVRLERPDLNFPALVAHPVFRPV
                     KVPDDPLKKIEPAKLISNGAFLLSKTEADRVLLERADNYWGRQQVALERVSFVATRNA
                     ETALAAYREGQVDAVTNAPFEPLAIKLLSPYQDFRRATYGALTYYSFNTDHAPFDDIR
                     VREALAIAIDRDRISQAEMGGATEPAKRFLPDEMTEHDRPVVGKSDLLEKDGARAREL
                     LAEAGYPEGRDFPKIRLLVNRNEQQRIVSGSVAAMWREVLNIETEIVMKSWDEYESAI
                     RAGEYDVVRRGLVMQTTDELTNIRMLFRPTPSSPLAIENENSENANHPPEPAVASEEP
                     IESEEQALRELKAVPIYFASSYSLVKPYVVGF"
BASE COUNT          525 a          569 c          639 g          481 t
ORIGIN      
        1 cgggaagttg tcgttcctgg ctctccagga ctggcagcgg tcgtagaaac cttcggagca
       61 gatgttttag catccgacgg cacccttgat cgtaagaggc tgggtgaact ggtgtttcga
      121 gatgaagcaa agcgccggct cctaaactcg attctccatc ctttcatcat tgagcgtcag
      181 gaccaacaga tacgcgcgtg ggagtctgaa gatcccaacg gcatcgcggt tgtagatgcg
      241 gccctgatga tcgagtctgg tggctattca cggtttgata agctgatcgt cgtttactgc
      301 cggcctgaga ttcagttgca gcgaataatg aagcgggaag gtttctctct cgaggaagca
      361 aaaagaagga ttgctgctca aatgtctcag gaagagaaat tgaaattcgc cgactttagg
      421 atcgatacct caggcgagtt tgatcaaaca cgcatccagg tggaagaggt atatcgcgaa
      481 ctgcaattgc tctccggaaa acaacagttg aaccctgtgg cctctaaacg ggcgcgtttt
      541 gagacaccag gcaagaacag ataacgcgca tgccccggct tcgaatcaga aacatgaaga
      601 gactctcgca atgccgtagt gcggttgttg ccgttctgct gcttgcaatg tctcttagtc
      661 atgcaagctg tttcagtaac gaggcaggta gtccgttcta cggccgcgtt ttgtccccca
      721 agctccagga gtttcgctgg agcgatgggg gactgccgca gatcttcgat cccgcctttg
      781 ctgccgcccc tcccgataca gaccttgtgc gagccatctt tgaggggctc acagactatg
      841 acccgacaac cttgaggccg gttccagccg tagccacgcg ttgggagtca tcaaaagatg
      901 gaaaggtctg gacgttcttt ctacgagagg acgcgcggtg gtccaatggt gagttggtga
      961 cggcggacga ttttattcgg tcgtggcaac gcaccgtgag attgggtgac caggcaccgc
     1021 acacggagct cttacagaac attgagggag cctcggcggg aatcaacccg cctgtggcgc
     1081 ctgcaaggca gcagcgttcg tccaatgaga aggcgaggag tctgcccgcg cggagacagc
     1141 aaccttttgg cgccgaagca gtcggtacac atgtccttcg agttcgactg gaacggccgg
     1201 atctgaactt tccggcgctg gtcgctcatc cggtatttcg cccggtaaaa gtacctgacg
     1261 atccactcaa gaaaatcgag ccggcaaaac tcatctcaaa tggcgcgttt ctcttgtcca
     1321 aaaccgaagc agatcgcgtt ttgcttgaac gtgcggacaa ttactggggc aggcagcaag
     1381 ttgcactcga acgcgtatcg ttcgttgcta ccaggaacgc cgagactgct ttagcagcct
     1441 accgcgaagg gcaggttgat gcggtgacca atgctccctt tgaaccgttg gcgatcaaac
     1501 ttttgtcccc ctaccaggat ttccggcgcg ccacttacgg cgcgctgacc tactacagct
     1561 tcaatactga tcacgcgcca ttcgacgaca tcagggtccg cgaggctttg gcaattgcga
     1621 tagataggga ccgcatcagc caggcagaga tgggcggcgc tacagagccc gcgaagagat
     1681 tcctgcccga cgaaatgact gaacatgatc ggcccgtggt aggcaagtct gatctccttg
     1741 agaaggatgg tgcacgcgcc agagaattgt tggccgaagc gggctatccc gaaggtaggg
     1801 attttccgaa gatcagattg cttgtgaatc gcaatgaaca gcaacggatt gtttcgggat
     1861 cggtagctgc aatgtggcgt gaggtgctga atatcgagac cgagatcgtc atgaagagct
     1921 gggacgaata tgaatcggcg atcagggcgg gcgagtatga cgtggtccgg aggggacttg
     1981 tgatgcaaac tacagatgag ttgactaaca tccgaatgct gtttcgccca actccctcaa
     2041 gcccactggc catcgaaaac gaaaattcgg agaacgctaa tcatccacct gagccagcag
     2101 tcgccagcga ggagccaatc gaatcagaag agcaggcgct cagagagttg aaggcagttc
     2161 cgatctactt tgcatcgtca tactctttgg taaaacccta cgtggtcgga tttg
//
LOCUS       DASUEX010000518         3482 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_426059, whole genome shotgun sequence.
ACCESSION   DASUEX010000518 DASUEX010000000
VERSION     DASUEX010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3482
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426059"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            449..2050
                     /gene="lnt"
                     /locus_tag="VFV61_08675"
     CDS             449..2050
                     /gene="lnt"
                     /locus_tag="VFV61_08675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEX4900592.1"
                     /translation="MTNVAASPLAVNAQKGSLSSRVLTAARPFSDWLLALSCSLLLIL
                     AFPDFEIWPLAAVALVPLLIVVANRPKPWRSFFLGWLTGTVFFYGSCYWLTYSMIHYG
                     GVPTVLAYLSLIPVTLVVGLFPGLYAFVLARAIRRWGMAAILTAAFVWPAFEWARLGI
                     TGQLWNALGYSLAYQSFPIQAANWGGVYAVGFILVSINAAIAFLLLRRTAKAASVAIA
                     VIAVTVLVMLVSKELSGNFMRSSDAEVVVVALQPNVPMDLVKSTAEMQALMDRHLKTT
                     EETLNSLPPSDHARLVVWPESPMYFTYGTDTQLRELLMNFATTHNTSILFNSQEPAPN
                     DGIYNSALFVNQQGRLIAQYDKIRLMPFGEYVPLPRWMPGANLITAIVGEFTPGTRFT
                     LMPLGDSRAGVFICVESAYPYIARNLTNEGADVLINISNDGYLGPTPVRRQHLANAVF
                     RAVENGRPVLRVTNTGISAYITDRGDIHDATESFQPAVLTWGINGKASSQTFYTRHGD
                     VFVAVCALLSLVLLVSTFRFRTKHN"
     gene            2060..3170
                     /gene="prfB"
                     /locus_tag="VFV61_08680"
     CDS             join(2060..2131,2133..3170)
                     /gene="prfB"
                     /locus_tag="VFV61_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_319006089.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEX4900593.1"
                     /translation="MIETLSIQDLRTTYEDLLKRVEQLGGFFDVPKAREELNIIETQA
                     SVPDFWNDQDAAQKLLQKRSGLEKKIQRQEQFDAKIADAGVLFEFAEEDAESLNELRV
                     LVERLEQELGQAETEMLLAGENDQLNAICTIHPGAGGTESQDWAEMLLRMYLKWAEQR
                     GFKSEVIDYQPGEEAGLKSVTFRVEGEYAYGLLSAEAGVHRLVRISPFDQAARRHTSF
                     ASLFVYPEIDENIEVEVNDKDLRVDTYRATGAGGQHINTTDSAIRITHIPTGIVVQCQ
                     NQRSQHQNRAVAMQVLRARLYELEIEKRRADTAELEANKADISFGSQIRSYVLAPYQL
                     VKDTRTKLERGNVEAVLAGDLDDFIKAYLLAKKAA"
     gene            3210..>3482
                     /locus_tag="VFV61_08685"
     CDS             3210..>3482
                     /locus_tag="VFV61_08685"
                     /inference="COORDINATES: protein motif:HMM:NF024882.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEX4900594.1"
                     /translation="MATSQLEEKARIITPRNSRRNEIIAILFLALGLLLSLCLISAAF
                     NPNDPSWNSAGQTETNNWAGAIGASVAAAMFQFIGLAAYLLPVLFLA"
BASE COUNT          771 a          955 c          926 g          830 t
ORIGIN      
        1 cctgccgctg cgccagcccc ccgccgagca acccttcagt taacccttcg aaatgcgaac
       61 ccagtcagaa atggctgggt tctcccgcgt ttagagcgca actttttcac tgccgacagg
      121 cctaattgat ctctttagga caaactgggt attagcgacc tcgcccttac cgcgccagac
      181 tgacttaccc catcagtcag tctgcgacgt aatttgtcac tttgacgtga cgttttctgt
      241 cgatttcagc agtccagcgc accttttgag cggtcgcgag ttggggcgcg gtgaagatag
      301 agaaattttt ttacgggatc ctgattaacc atcgggggca agcgtctctt tccgaccgcg
      361 ctttggctcg actcgccttt tggctcgact gctttgcgac aggaggggca cttcgccgga
      421 atcaaacttt gctaaactcg cgcgcagcat gaccaatgtt gccgcgtccc cgctcgcagt
      481 gaacgcccag aaaggaagtc tgtcctcccg agttctcacg gcggcacgcc cattcagcga
      541 ctggctcctc gccctcagct gctcgctttt actaatcctt gcgtttcctg atttcgagat
      601 ttggccactc gccgctgtcg cgttagtgcc tctcctgatt gtggtcgcaa ataggccaaa
      661 gccctggcgc tcgttcttcc tcggctggct gacgggaacg gttttctttt atggctcgtg
      721 ctactggctg acctattcaa tgattcacta cggcggcgtc ccgaccgtac tagcctatct
      781 ttccttaatc cccgtgacat tggtcgttgg cttgttcccg ggcctgtatg cgtttgtgct
      841 cgcgcgagca atcagacgct ggggtatggc agccatactc acggctgcgt ttgtgtggcc
      901 ggctttcgaa tgggccaggt tgggaatcac gggacagctc tggaatgcac tcggttattc
      961 attggcctat cagtctttcc cgattcaagc tgctaactgg ggtggagtct atgccgttgg
     1021 cttcatctta gtgtcgatta atgctgcgat agcttttctc ttgttgagac gaaccgccaa
     1081 agcggcaagc gtcgcaatcg cggtaatcgc cgtgactgtc ctggtcatgc tggtttccaa
     1141 ggaactgtcg ggaaatttca tgagatcttc ggatgccgaa gtagtagttg tcgcgttgca
     1201 gcccaatgtg ccgatggatt tagtgaagtc taccgctgag atgcaagcgc taatggaccg
     1261 tcacttgaaa acgactgagg agacgcttaa ctcgttacct ccatccgacc acgctcgact
     1321 tgtagtttgg cccgaatcac cgatgtactt cacatatggg actgacacgc aattacgtga
     1381 actgctgatg aacttcgcga caacgcacaa cacttcaata ctctttaact cgcaggaacc
     1441 ggctccgaac gatggcattt acaactctgc gctcttcgta aatcagcaag gtcggctgat
     1501 cgctcagtat gataagattc gcctgatgcc gtttggcgaa tacgtgccgc tcccacgctg
     1561 gatgccggga gcgaatttga taactgccat agtgggagag ttcacgccag gcactcgttt
     1621 cacgctgatg cccctaggtg actcgcgcgc cggagttttc atttgcgttg agtctgccta
     1681 cccgtacatc gcgcgaaatc tgacgaacga aggcgccgac gtgctgatca atatttccaa
     1741 cgatggttat cttgggccga cacctgtacg ccggcagcat ttggcgaacg cagttttccg
     1801 cgccgtagag aacggccgac cggtactacg tgttactaat accggaatca gcgcgtacat
     1861 caccgaccgg ggtgatattc acgatgcgac agagagcttt caaccagcag tgctaacttg
     1921 ggggatcaac ggtaaagctt caagtcaaac attctatacg cggcatggcg acgtcttcgt
     1981 cgccgtctgc gcgttgttaa gcctggtttt attggtgagt acctttagat ttcgcaccaa
     2041 acataattag aggaaacgaa tgatcgagac gttatcaata caggacttgc gcacaaccta
     2101 tgaagacctc ctcaagcgag tagaacaact cgggaggttt ctttgatgtc cctaaagcgc
     2161 gcgaggaact gaacatcata gaaacccagg cttcggtgcc tgatttctgg aacgatcagg
     2221 acgccgcgca gaagttatta cagaagcgca gcggcctgga aaaaaagatt cagcggcagg
     2281 aacagtttga cgcaaagata gccgacgcgg gcgtgctgtt tgaattcgcg gaggaggatg
     2341 cggaatcgct gaacgagttg cgcgtgctgg ttgagcgttt ggaacaagag ttaggccaag
     2401 ccgagaccga aatgctcctc gccggcgaaa acgatcagct aaatgccatc tgcactattc
     2461 atcccggtgc aggcggaacc gagtctcagg attgggccga gatgctcttg cgcatgtacc
     2521 tgaaatgggc cgagcagcgc ggctttaaga gtgaagttat tgattatcag ccaggtgaag
     2581 aagcaggttt gaagagcgtt actttccgcg ttgaaggcga atacgcctat gggctgctct
     2641 ctgccgaagc gggtgtccat cgtctcgtca gaatttctcc tttcgatcag gcggctcgca
     2701 ggcatacctc tttcgcttcc cttttcgtct atcccgaaat cgacgagaac attgaagttg
     2761 aagtcaacga caaagatctg cgtgtagaca cttaccgcgc gaccggcgcc ggcggccagc
     2821 atatcaacac caccgactct gcgattcgca tcacacacat tcccaccggc attgtcgtgc
     2881 agtgtcagaa ccagcgttcg cagcaccaga atcgggctgt agcaatgcag gtcttgcgcg
     2941 cacgactgta tgaactcgag attgagaagc gccgcgcgga taccgccgag ttggaggcta
     3001 acaaggccga catttctttt ggctcgcaaa tcagaagcta cgtacttgct ccataccagc
     3061 tcgtgaagga cacgcgcacc aaactggaac ggggaaatgt cgaggcggtg ctggcaggcg
     3121 acctggacga tttcataaaa gcctacctct tagcgaagaa agcagcgtaa actgcgggcg
     3181 gacttgaagt cagtttcgtt ggagtgttga tggccactag tcagcttgag gaaaaagccc
     3241 ggatcatcac gccgcgcaac agccggcgaa atgaaatcat cgccattcta tttctcgccc
     3301 tcgggttact gctttcgctt tgtctgatct ccgcagcctt caatccaaac gacccttcct
     3361 ggaattccgc cggacaaacc gaaaccaaca actgggcagg agcgatcggc gcttccgtcg
     3421 cggcagcgat gttccaattc attggactcg ccgcctacct attgccggtg ctgtttttgg
     3481 cc
//
LOCUS       DASUEX010000519         3888 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1591913, whole genome shotgun sequence.
ACCESSION   DASUEX010000519 DASUEX010000000
VERSION     DASUEX010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3888
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1591913"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            328..2112
                     /locus_tag="VFV61_08690"
     CDS             328..2112
                     /locus_tag="VFV61_08690"
                     /inference="COORDINATES: protein motif:HMM:NF015557.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEX4900595.1"
                     /translation="MTTQIAIVLSLLLVALVLFALERIPIEIVSIVLVMALIITQTLT
                     VAEAFAGFGNDIVITIAGLFILTGGLAKTGVVDVVGRRLHRFAGEGEFTITALIMVAA
                     ALSAAVMKNTTTTAMFLPVVLGIGARRNISPSKLLIPLAFGAILGGTCTLIGTSTNLA
                     VSGALPRYGMEPFTMFELTKVGVPIVGVGMLYMLLLGLRLLPRREAAKSLTEQYHVRQ
                     YMTEVIVLDNSPLIGKTLAAARIGDELDLTVLGILRGNQQYRIAPHPREQIKAGDLLL
                     VQGRVEDILRVKSEAGIEIKADFALSDELLEGADVELFEAMVPRGSDFIGRTLKRLQF
                     RRRYQLVVLAINRHGVNLLSKLSKVKIRFGDVLLIQGNREHVENLATDDQILLLEEIS
                     ERQARPKKMRWALLAFSVFLFFSITHLVPLPVAVLIGVLILLATRSLLTAEIYEMIDW
                     RLIILIACMISFGVAMEKSGADQYLAGFIIRGTAHYGATAVLAGFFIMTVALTQPMSN
                     QAAALVMLPIAAKSALALGLNPRTFAVAVTYAASCSFLTPLEPACVLVYTPGRYRFFD
                     FVKVGSILTIVVFAIVIWLVPVFWPLTP"
     gene            complement(2332..3015)
                     /locus_tag="VFV61_08695"
     CDS             complement(2332..3015)
                     /locus_tag="VFV61_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900596.1"
                     /translation="MMFLRNRLTSSFCLGMVLLSLTFTFSCQPTTGPTPNSNYNANSS
                     PTAPDPRAELVRSLKGANVDGFHFATREDDNQTITSLTNGALRSPVKLDDGGTFVLGY
                     ANVRDKKTNTTKTLQAEIVRSGSAYSLHLKDIVAGGLVIDERFAPRICPGVPVFNTFD
                     ECFDDFECKVRPELLCEANRTCRNIYTEVQCCLRDGTRIHALVIIRPTARRCVAVFPL
                     DINEVLLYP"
     gene            3578..>3888
                     /locus_tag="VFV61_08700"
     CDS             3578..>3888
                     /locus_tag="VFV61_08700"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX4900597.1"
                     /translation="MTNPSFNTAVTELSLRFSGELLQSTDAAYEDARRVHNGLIDKRP
                     LLIARCHGVADVVDAVKLARTLNLEVAVRGGGHNVAGRATIDDGLMIDLMPMKGIHVN
                     PR"
BASE COUNT          820 a         1054 c         1068 g          946 t
ORIGIN      
        1 tcaggaaatc agccagcagc tggaggcgat cgtaaacaac gctgacaagt gcctggagtt
       61 gctgaatgaa gacaagcccg acatttcgct tttccgcgca ccgcttaaga ccatcgccag
      121 ctccggtcgc caggcccagc agatcatcaa taagctccga ccaccgggga agtagcgaac
      181 actaatagtc ccaaagtcca atgtccaaag tccaaggtcc tcgtgcctga gcattgctct
      241 gacctggaca gattccaggc tttgaggaca gcggactttg ggttttggac tttgaaccaa
      301 tccccgcgcc cgattgtgcc taattagatg accactcaga tcgctattgt cctcagcttg
      361 ctgctcgtcg cactcgtcct ctttgctctt gagcgcattc ctatcgagat cgtttccatc
      421 gtcctcgtaa tggctttgat catcacgcaa accctcacag tggctgaggc gtttgccggt
      481 tttggcaatg acattgtcat caccatcgcc ggcctcttta ttctgaccgg cggtttggcc
      541 aagaccggag tcgtcgatgt ggtgggcagg cgtttgcacc gcttcgccgg cgagggtgag
      601 tttacgatca ccgccttgat catggtggcc gccgccctct ccgcagccgt catgaaaaat
      661 acaactacga ctgcgatgtt tctgccagtc gtgttgggca ttggcgcgcg tcgcaacatc
      721 agcccttcaa aacttctgat cccgctcgct ttcggcgcca tcctcggcgg cacctgcacg
      781 ttgattggaa catccaccaa ccttgcagtc agcggcgccc tgccccgcta tggcatggag
      841 ccgtttacga tgtttgagtt gacgaaagtt ggcgtgccaa ttgttggcgt cggcatgctc
      901 tacatgttgc tgctcggcct gcgtcttttg ccgcgccggg aagcggcaaa gtctctcacc
      961 gagcaatatc acgtgcgcca gtacatgacc gaagtgatcg tgttggacaa ttcgccgttg
     1021 attggtaaga cgctggccgc ggcgcgtatt ggcgacgaac ttgacctgac ggtcctcggc
     1081 attttgcgcg gcaatcagca ataccggatt gcgccgcatc cccgcgagca aatcaaggcc
     1141 ggcgatctgc tgctcgttca gggacgcgtg gaagacattc tcagggtaaa gagcgaagcg
     1201 gggatcgaga tcaaggcgga ctttgctttg agtgatgaac tgctcgaagg agccgacgta
     1261 gaactattcg aggcgatggt gccgcgcggg tcggatttta ttggccggac tttgaaacga
     1321 ctgcagtttc gccggcgcta ccagctagtg gtgctggcaa tcaatcgcca cggcgtaaac
     1381 cttctctcga aactaagcaa ggtaaaaata aggtttggtg atgtgctctt gatacaggga
     1441 aatcgcgagc acgttgagaa cctcgccacc gacgatcaga tcctcctgct tgaagaaatc
     1501 tctgagcgcc aggcgcgccc gaagaaaatg cgctgggccc tgctcgcgtt tagcgtcttt
     1561 ctatttttct caatcaccca cctggttccc ttacccgtcg cggtgctgat cggggtctta
     1621 atactcttgg ccacgcgctc gctgctcacg gctgagattt acgagatgat tgactggcgc
     1681 ctgataattc tgatcgcctg catgatcagt tttggcgttg ccatggaaaa gtcgggcgcg
     1741 gatcagtatc tggcgggttt cattatcagg ggcacggctc attacggtgc tacggcggtg
     1801 ctggcagggt tcttcatcat gaccgtcgcc ctgacgcaac cgatgtcgaa ccaggctgca
     1861 gcactcgtga tgctgccaat tgccgcgaag tccgcgctcg ctttgggatt gaatcccagg
     1921 acgttcgccg tggcagtaac ctacgcggct tcttgttcct tcctgactcc gctcgagcct
     1981 gcttgcgtgc ttgtctacac cccggggcgt tatagatttt ttgacttcgt aaaggtgggc
     2041 tcgatcctga cgatcgtagt gtttgcgatt gtgatttggc tggtgccagt gttttggccg
     2101 ctcacgccat aggcgtctca gggcccaagt ctcaggtctc aggtttcaag tttcagatct
     2161 caggtttcaa gtttcatgtt tcaggttttg atcgcgctga aggcgcgtgc agaaggtagc
     2221 caggggcaag tcccgacttg tcgggacgcc gcccctggat cacgaacctc acaatttcta
     2281 gaaagggtga tatcgaatcg gcaatcggca atcggcaatc gccaatcccc attacggata
     2341 cagcaacacc tcattaatat ccagcgggaa tactgcgacg catcttctcg ctgtcggcct
     2401 gatgatgact agcgcatgaa tgcgcgtgcc gtcccgcaga caacactgaa cctcagtgta
     2461 gatattccta caggtgcggt tggcttcgca tagcaactcc ggtcgcactt tgcattcgaa
     2521 atcgtcaaag cactcgtcaa aagtgttgaa gactggcact ccgggacaga ttcgcggcgc
     2581 aaaccgctca tcaatcacca ggcctccggc cacaatgtct ttcaggtgca gactgtaagc
     2641 gctaccactg cggacgattt cagcctgcag tgtcttcgtg gtgtttgtct tcttgtctct
     2701 tacgtttgca tatcccagga caaacgtgcc gccgtcgtcc agctttaccg gactgcgcaa
     2761 ggcgccattt gtaagcgaag tgattgtctg gttgtcatct tccctggtgg caaagtggaa
     2821 accgtcaacg ttcgcgcctt tgagcgagcg cacgagttct gctctcggat ccggcgcggt
     2881 gggactgctg ttagcgttat agttggagtt gggtgtcggc ccggtggtcg gttgacacga
     2941 aaaggtgaaa gtgagagaca gcaacaccat tcctaaacag aatgaggatg tcaggcgatt
     3001 gcgtaagaac atcatcttag cctcctagtg attggtgcga gggttgagtg agttatgtcg
     3061 ttaagtcaga accgggagcg gtagcgaccg ggtcatcacc gcagcctttt aggtcagaac
     3121 cgagagcgat agcgatcgga tagtcacaga agctttcaag tgcgaggatt tgctccggcg
     3181 gctgcgcgct tcttatatct cactcccacc ttgtttacaa agaatcggcg acagcgatgt
     3241 ctcgccgagg cgaaaaacgg aaccacttat tcacacgaag caacactaaa tgcattcttg
     3301 cgttagtgtc gttttagtgt gatttgtggt tctctgcttt gaatccattt gcgggaggaa
     3361 gaatcggcaa ttctcaaccc gtgccgatgc cggtgggatc gagaccgaat taataatcca
     3421 cgggcggcta ccaggatgtg tgcaagtcca ttggcttgcg cacaagtctg caatcaaatg
     3481 aagtagtcta aattcagttg cgctgagcac tacctcagga actatacttt ccgcctcgcg
     3541 cagtagtttt tagtgaccca cataaggagg actccccatg acgaatccct ctttcaacac
     3601 tgccgtaacc gagctcagtc ttcggttttc tggtgagctt ctgcagtcca ctgatgccgc
     3661 atatgaggat gctcgcagag ttcataacgg tctaattgac aaacgtcctt tgctgattgc
     3721 gcgctgccat ggcgtcgctg acgtagtcga tgcagtaaag ctggcgcgca cgctgaatct
     3781 tgaggtcgcc gtccgagggg gcgggcataa tgttgccggg cgcgcaacca tcgatgacgg
     3841 actgatgatt gacctgatgc cgatgaaagg catccatgtg aatccccg
//
LOCUS       DASUEX010000520         4664 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1470657, whole genome shotgun sequence.
ACCESSION   DASUEX010000520 DASUEX010000000
VERSION     DASUEX010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4664
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1470657"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="VFV61_08705"
     CDS             <1..256
                     /locus_tag="VFV61_08705"
                     /inference="COORDINATES: protein motif:HMM:NF024763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEX4900598.1"
                     /translation="SKIKRLPDPNDKVKENKPGAYYVDSSCIDCDVCRDTAPESFRRS
                     DENSYSFVYKQPETPEETAACEEALTCCPVEAIGNDGEEQ"
     gene            438..1421
                     /locus_tag="VFV61_08710"
     CDS             438..1421
                     /locus_tag="VFV61_08710"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEX4900599.1"
                     /translation="MKRLLTICGVLTLLTFFAAPIISSASATEINKTVRGRLQRTVEA
                     GGWLVVDNNQKFLLINAQRFQNEGWFREGVEVEAIGETRDVMTVYMEGTPFEAQSLKP
                     LNAQAGNQIVPGGDRRITRVIVSGDSLVQAQPDTAILTVSVVTQAKNALDAQQQNAAR
                     TDAVVRTLKSAAGTGAEVKTSGYSLQPQRIYRENQPPTITGYEARNTVTVTVGDLTKV
                     GPVIDAAAQSGANDIGGVAFTLRKDRPARDEALALATREAMSKAQVIAQALGGRVVRI
                     VEVQEEGTVRPRPVYDAEMVRGMAQKSVATPIEIGSLEITGRVQIVAEVES"
     gene            1673..2029
                     /locus_tag="VFV61_08715"
     CDS             1673..2029
                     /locus_tag="VFV61_08715"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX4900600.1"
                     /translation="MPEPKRAFSMMALRNQVRPSGRLWNPNADVYQSSEGWVVKVDLA
                     GVCPDELELVLQDSHLIVRGCRRDTLYREGFTYQQMEITYSRFEKRIDFPCSIESSSL
                     KQDYRDGFLIVSLSCT"
     gene            2125..4590
                     /gene="lon"
                     /locus_tag="VFV61_08720"
     CDS             2125..4590
                     /gene="lon"
                     /locus_tag="VFV61_08720"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEX4900601.1"
                     /translation="MPAPSATEPRPAPGEPFELAVLPLQNTTLFPETVVPLAIGRSRS
                     IAAVQAALATEEKLLACISAREQSGDHDATPADLYEIGTLVMIKRMERIEDAMHIIAQ
                     GTERIKVLEWKQEEPFLRARVQILPEPRIVDAEEVEAMRRNVQSMIQQALALLPGVPP
                     EVRVAMLGSVEPVRLAYFLGSILNLGTEQEQKMLEAETADELLRLSHGYLARELEIIQ
                     LRSKITTEAQSEMDKAQREYVLRQQMKAIQKELGEDEGGERAETELLRERLAGADLPA
                     EVRTEADRELKRLERLPAAAPDYHVIRTYLEYIIELPWKKSSEDKLDLIEARKILDED
                     HFGLDDVKERILEFLAVIKLRPDTKSPILCFVGPPGVGKTSLGRSIARALGRQFERLS
                     LGGMRDEAELRGHRRTYIGSMPGRIIQSLRRAGVNNPVMMLDEIDKLGNDYRGDPAAA
                     LLEVLDPQQNNTFRDHYLDLPFDLSRVFFIATANQMGPIPPPLRDRMEVIYLAGYSDP
                     EKLQIAKRYLVPRQTTENGLKAEQLQVSDDAIELIAGRYTREAGVRQLERTIGRVARK
                     VALKVAKGEAQSVAVTAATIPEYLGPPRFYPEQARKELPAGVATGMAWTEMGGEVLFI
                     EATLLPGGSGLTITGQLGEVMQESARAARSYLWSHAIEFGINPEMFKDYGVHLHVPAG
                     AIPKDGPSAGVTITAALASLYTGRRVRPDTAMTGEITLSGLVFPIGGLKEKAMAAHRA
                     GIRRLIIPARNEADVEDIPDDVRADLEIVFVSRINEVIDGALEVLVANPPPPPLVTPA
                     SLNQSSRAPDEPISAPQSLKTST"
BASE COUNT         1105 a         1246 c         1388 g          925 t
ORIGIN      
        1 atcgaagatc aaaagattgc cagatccaaa cgacaaagtt aaagaaaaca aacccggcgc
       61 ctactacgtc gactcgtcgt gtatcgattg cgatgtttgt cgcgacactg cgccggagag
      121 tttccggaga tcggacgaga acagttattc cttcgtttac aaacagccgg agaccccgga
      181 agaaacggca gcctgtgaag aagccctcac ctgctgtccc gtcgaagcca tcggcaatga
      241 tggcgaagaa cagtaggcag acggcagttc cgagctttaa gtcccaagtg ccaagtgcaa
      301 agctctccaa gtacaaagtt ccaagttcct ggttaagagt ccgcaagaat ccaatccgcg
      361 agcgttctct atctcggcgc tagtactttt tctcttcccc ggcgcagaat gaagcaaata
      421 cgggagggga ttagaacatg aagagactat taactatttg cggcgtgctt acgctgctta
      481 ctttttttgc agcaccaatt atctccagcg ccagtgccac tgaaattaac aagactgtgc
      541 gcgggcgttt gcagcgaacc gtcgaagcag gtggctggtt ggttgttgat aacaatcaaa
      601 aatttttgct gatcaacgca cagcggttcc aaaacgaagg ctggtttaga gaaggagttg
      661 aagtcgaggc gattggcgaa accagggatg tcatgacggt ttacatggaa ggcacaccct
      721 tcgaggccca gtcgctaaag ccgctaaacg cgcaagcggg caatcaaata gtgcccggtg
      781 gtgatcggcg gatcacacgg gtgatcgttt ccggagattc gctggtacag gcgcagccgg
      841 acacagcgat tctgacagtc tcagttgtga ctcaggccaa gaatgcgctt gacgcccaac
      901 aacaaaacgc agcccgcacg gatgccgtgg tgcggacctt gaagagtgcc gcggggaccg
      961 gggccgaagt caaaacctcc ggctacagtc tccagccaca aaggatttac cgggaaaacc
     1021 aaccaccaac gatcaccggt tatgaagccc gcaacacggt gactgtcacg gttggagacc
     1081 tgaccaaggt cggacctgta atcgatgcgg cggcgcagtc tggcgctaat gacattggcg
     1141 gtgtggcgtt cactttgcgg aaagaccggc cggcgcgaga tgaagcgctt gctttggcta
     1201 cgcgagaagc gatgtccaag gcgcaggtaa ttgcgcaggc gttgggagga cgcgtagtgc
     1261 ggatcgtgga agtgcaggaa gaaggaaccg tacggccgcg gcccgtttat gacgctgaga
     1321 tggtgcgtgg catggcccag aaatcagtcg ctactccgat cgagattggc agcctggaaa
     1381 taaccggccg ggtgcagata gtggcggaag tggaaagcta agagggacgg tgagacgcgg
     1441 agacgaggag acacggagac gcaagacgcg gagacacgga gacgcggaga cacggagaca
     1501 cggagacacg gagacgcgga gacacggaga cacggagaca cggagacggt gaaacgcggc
     1561 gacgcggaga aagggagacg cggcgacagg gagtccccca atctccgcgt cgccgtgtct
     1621 ctttccccct tccccttttc ccctttttcc ctttttccct tatccttaac gcatgcccga
     1681 accaaagcgt gctttttcga tgatggcgtt gcgcaaccag gtgcgaccct ctggccgtct
     1741 ctggaatccc aacgccgatg tttatcagag ctccgaaggt tgggttgtga aagttgatct
     1801 cgccggggtc tgtcctgatg agttggaatt agtactccag gattctcacc tgatagtgcg
     1861 tggttgccgc cgcgacaccc tttatcgcga aggctttaca taccagcaga tggagatcac
     1921 gtatagccgt tttgagaaac gtatcgactt tccctgttca attgagtcgt cctccctcaa
     1981 acaagactat cgcgatggat tcctgatagt cagcctgagt tgtacgtagt cgaggtaggg
     2041 agtccagacg agaccatgcc ggaaaaccca aagaaaaccg caagtaccag tacaccggag
     2101 ccgcctaaaa aacccagatc tgtggtgcca gccccgtcag caaccgaacc ccggcccgca
     2161 ccaggcgaac cgttcgagtt ggccgtgttg cctttgcaaa acaccaccct gtttccggaa
     2221 acagtagtgc cactcgcgat tggccgttcg cgttccatcg cggccgtgca agccgcgctc
     2281 gcgactgaag aaaaactatt agcctgtatc agcgcccgtg agcagagcgg cgatcacgac
     2341 gcgacccctg ccgacctcta tgagatcggc accctggtaa tgatcaagcg gatggagcgc
     2401 attgaggatg caatgcacat catcgcgcag ggaaccgagc gcataaaggt actcgagtgg
     2461 aaacaggagg aaccttttct gcgggcgcga gtgcagattc ttcccgagcc acgaatcgtg
     2521 gacgcagaag aagtcgaggc catgcgccgg aatgtccagt cgatgatcca acaggccctg
     2581 gcgctgctgc ccggtgttcc tccggaagtt cgcgtcgcaa tgttgggttc agtcgaacct
     2641 gtgcggctcg cttacttcct cggctcgatc ctgaacctgg gtactgagca agagcagaag
     2701 atgctcgaag cagaaaccgc agacgagttg ctgcgtctgt cgcatggtta tctggctcgg
     2761 gagctggaaa tcattcaact gcgttcgaag atcacgactg aagcgcaatc ggagatggac
     2821 aaggcgcagc gcgaatatgt tttgcgccag cagatgaagg caatccagaa ggaattggga
     2881 gaagacgagg gcggcgagcg tgcggaaacc gagctgctgc gcgagcgtct ggcgggcgcc
     2941 gacttacctg ctgaagtgcg caccgaagca gaccgcgagt tgaagcggct ggagagatta
     3001 ccggctgccg cgcctgatta ccacgtgatt cgtacctacc tcgagtacat catcgagttg
     3061 ccctggaaga aatcttcgga agacaagctg gatctaattg aagcaagaaa gattctcgac
     3121 gaagatcact tcggacttga tgacgtcaag gaacgcatcc tcgaattttt agcggtgatc
     3181 aagctgcgcc cggataccaa gagtccgatt ctttgtttcg tcgggccacc gggagtgggc
     3241 aagacttcac tcggtcgctc gattgcgcgc gcgttaggcc ggcagtttga gcgcctctca
     3301 ctgggcggta tgcgcgacga agccgaacta cgcgggcacc gtcgcaccta cattggttcg
     3361 atgccgggtc gcatcattca atctttgcgt cgcgctggag ttaacaatcc agtgatgatg
     3421 ctcgatgaga tcgacaagct gggcaacgat taccgcggag atccggccgc ggcgctgctc
     3481 gaagtcctgg atccgcaaca gaacaacact tttcgtgatc actatcttga tctgccgttt
     3541 gatctctcaa gagtgttctt tatcgccacc gccaaccaga tgggcccgat tccgccaccg
     3601 ctgcgtgatc gcatggaggt gatttatctt gccggctata gcgatcccga gaagttgcaa
     3661 attgccaagc gttacctggt gccgcgacag accactgaaa acggattaaa ggcagagcaa
     3721 ctacaagtct cagacgatgc tatcgagttg attgcggggc gatacacgcg cgaagccggc
     3781 gtgcgccagc ttgagcgcac catcggccgg gttgcgcgaa aggtagcttt gaaggtcgcc
     3841 aagggcgaag cgcagagcgt cgcggtgact gcggccacaa tcccggaata tctgggacca
     3901 ccccgcttct atcccgaaca ggcgcgcaag gagttgccgg cgggagtagc gacgggcatg
     3961 gcctggactg agatgggtgg cgaggtgttg tttattgaag caacgctgct gccgggcggc
     4021 agtggcctaa ctattactgg gcagctgggc gaagtcatgc aggaatcagc gcgcgcggcc
     4081 cgctcctatt tgtggtcgca cgcaattgag tttggtatta atccggaaat gtttaaggac
     4141 tatggagtgc acctgcatgt gccggcggga gcaatcccca aagacggtcc gagtgccggg
     4201 gtaacgatca cggccgcgtt ggcatcgctt tatacaggca ggcgtgtgcg acccgacacg
     4261 gcgatgactg gagagataac actttccggt ttggtgtttc cgattggcgg gctgaaagag
     4321 aaggccatgg ctgcgcatcg cgccgggatt cgccggttga ttattcccgc gcgcaatgag
     4381 gcggacgtgg aagacatacc agacgacgtg cgcgcggatc tggagattgt ttttgtttcg
     4441 cgcatcaatg aagtaatcga tggcgcgttg gaggtcttag ttgcgaaccc gccaccaccc
     4501 ccgcttgtta cacctgcttc tctgaatcag agctcccggg ctcccgatga gccaatttca
     4561 gcacctcagt cgctgaagac atcgacataa gacagaagtc agaagacaga agtcagcgct
     4621 gactactgcc tacctaccgg ctactgacta ctgacttatc ggtg
//
LOCUS       DASUEX010000521         3635 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_903038, whole genome shotgun sequence.
ACCESSION   DASUEX010000521 DASUEX010000000
VERSION     DASUEX010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3635
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_903038"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="VFV61_08725"
     CDS             complement(<1..284)
                     /locus_tag="VFV61_08725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900602.1"
                     /translation="MNKQKVTVEETAEKPAFVVGDRVEKLCVKCGEERGHVIASLTKR
                     GKISRVTCPKCGTVGSFKSGAATGTRRKLDPGAPYDQTRAYRTGQTMMHPT"
     gene            complement(302..523)
                     /gene="infA"
                     /locus_tag="VFV61_08730"
     CDS             complement(302..523)
                     /gene="infA"
                     /locus_tag="VFV61_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEX4900603.1"
                     /translation="MAKDDLIPAEGTIIDKQPNAFFKVQLENTDHVVLAVISGKMRKN
                     RIRILVGDRVSVEMSPYDLTRGRITYRYK"
     gene            complement(633..1904)
                     /locus_tag="VFV61_08735"
     CDS             complement(633..1904)
                     /locus_tag="VFV61_08735"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX4900604.1"
                     /translation="MNFSQLGLAPAQVRSCESLGYTEPTLIQQQAIPVVLSGQDLIGC
                     AETGTGKTAAFLLPIIQRITEAGPATAAPRRKPTSRASAKARVLVLAPTRELAAQIEK
                     NYSELNAAKSNRSVTLIGGASMVRQIDELRRNPAVIIATPGRLLDLVERGSVNLSAIE
                     VLVLDEADRMLDMGFLPAIRRVLALVPEKRQTLLFSATMSSEIESLARSTLNSPKLIE
                     VSQRGKPAAMVEQTAYPVAQESKTALLLDLLERERFERVLVFTRTRRGAERLSHILSA
                     RDHSVNRIHADRTQSQRESALRGFRDGRTRVLVATDIAARGLDVDLVSHVINYDVPSA
                     PEDYVHRIGRTGRAGNQGRAITIVTPVDELSMRAIERLTGESVKRVVLPGFGGTHHQT
                     SVPSRATNGRRAAGVSSGVFTRSFRPRRASR"
     gene            complement(2185..2568)
                     /locus_tag="VFV61_08740"
     CDS             complement(2185..2568)
                     /locus_tag="VFV61_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEX4900605.1"
                     /translation="MTMKLYVGNLSFSTSSQDLQELFAAAGTVESASVVEDRDTGRSR
                     GFGFVEMASKEEGEAAIAQFHGKEVNGRSLTVNEAKPRENRGGGGGRGGYGGNRGGGG
                     GYGGNRGGGGGYGGGGGGGNREPRW"
     gene            2919..>3635
                     /locus_tag="VFV61_08745"
     CDS             2919..>3635
                     /locus_tag="VFV61_08745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900606.1"
                     /translation="MPVILKFTITLLALAACLLASVNAQSKQPKQKKPVNELARLQEE
                     FVNATKDYKASLGKLLATYERDVTKAEAHLTQTRALFDQGLIAKAQLAQSETALAAAK
                     DKVTETSRLMVNADTQIAQTLLEAQAEEQLARTRLAKGRTLRTASYIRYAGGGGWLLS
                     DAWKVQRFFLDSFKKQLPIAVFGQGTIHDRWRLDHRNSMDISLHPDGPEGQALLNFLR
                     NNGIPFLAFRGAIPGTATGPH"
BASE COUNT          826 a         1058 c          944 g          807 t
ORIGIN      
        1 gtcgggtgca tcatggtttg tccggtgcga taagcacggg tttggtcgta gggcgcgccg
       61 ggatccagtt tacgtcgcgt tccagtggca gccccgcttt tgaagcttcc gacagttccg
      121 catttaggac acgtaacccg cgagatcttg ccacgcttgg taagggaagc gattacgtgg
      181 ccacgttctt cgccacactt cacacaaagc ttttcgacac gatctccaac cacaaatgct
      241 ggtttctccg cggtttcctc aacggtaact ttttgcttat tcacgatgta ctcctttatt
      301 gctatttgta gcgataagtt atgcgacccc tggtcaaatc atagggcgac atttcaacgc
      361 ttacgcgatc gcccaccaga attcgaatcc ggttcttccg catcttgccg gagatgacag
      421 ccaacactac gtgatcagtg ttctcgagct gcactttaaa gaatgcgttc ggctgcttgt
      481 cgatgatcgt gccttcagcc ggaatcaaat cgtcttttgc caatcaattt ctccttaaaa
      541 agggtagcgg gtttcgggtg tctggtgcca ggaagcgtta ggttcggttt caaactgaat
      601 ccgaaaccca ataccgggca cctgatacct cgttagcggc tagcgcgacg cggccggaat
      661 gaccgagtaa acactcccga agaaacacct gccgcacgcc tgccatttgt cgcgcgcgag
      721 ggaacgctag tctgatgatg tgtgccgcca aaaccgggca gcaccactcg cttaacgctt
      781 tcgccggtca agcgctcaat cgcacgcatc gacaactcgt cgactggtgt cacgattgtg
      841 atcgcacgac cctggttccc agcgcggccg gtgcggccaa tccggtgcac gtagtcttcc
      901 ggcgccgaag gcacatcgta attgatcaca tgcgaaacta aatcaacatc gagtccgcgc
      961 gcggcgatgt cagtcgcgac cagaactcgc gtacgtccat cccgaaaacc acgtagcgcc
     1021 gactcgcgct gcgattgggt ccgatcggcg tggatgcgat taacactatg atcccgcgcc
     1081 gataagatat gcgaaagcct ttcagctcca cgtcgtgttc gcgtaaacac caggacgcgc
     1141 tcaaaacgtt cccgctccag caagtccagc aacaacgccg tcttcgactc ttgcgccacg
     1201 ggataagcag tttgctcgac catcgcggct ggtttgccac gctggctaac ctcaatcagt
     1261 ttcggactgt tcagagtcga acgcgccaga ctttcaatct cagaagacat cgtcgccgag
     1321 aatagtaagg tctgtcgctt ttccggtacg agagccaaaa ctctgcgaat cgccggcaag
     1381 aatcccatgt ccagcattcg atccgcttcg tccaaaacca gaacttcgat ggcggaaaga
     1441 ttcactgaac cacgctcaac tagatccagc agtcgccccg gcgtcgcgat gatcaccgct
     1501 gggttgcgcc gaagttcgtc aatctgccgc accatgctgg cgccgccaat caaggtaacg
     1561 ctgcgattgg acttggccgc atttagctcg ctgtagttct tttcaatttg cgcagccaat
     1621 tcacgcgttg gtgccagcac aagcactcga gctttcgctg acgccctgga agttggctta
     1681 cgacgtgggg ccgcagttgc aggaccggcc tcagttatcc tttgaattat gggaagtaag
     1741 aaagcggcgg ttttgccggt gccagtctcg gcacaaccga tcaaatcctg tccgctaaga
     1801 acaacaggaa tcgcctgttg ttgaatcaat gttggttcgg tatacccgag agactcgcag
     1861 ctccgcacct gtgcgggcgc aagccccagt tgagaaaaat tcataaactc cttttgcgca
     1921 aacgctacag tcaaattctg aggtgcgcat ctttttacga tggtcggacg catttggcgt
     1981 ctaaccgaaa tttgcattcg cgatcaggca gacgagaacc gcaaagcgga ctgtcatgtg
     2041 ccgctcgacc tatgtcgggc cgcgagtgtt tgaggtttac ggtaagtccg taaggcgatt
     2101 cgccaggcaa gcttcttaag agctagtgag cttctcgcct tacggatcgg taccgttaga
     2161 aagaacgatt cgtcgcttca gcaactacca gcggggttcg cgattgccac cgccgccacc
     2221 gccgccgtaa ccgccaccgc cgccacgatt gccgccgtaa ccgccgccac cgccgcgatt
     2281 gccgccgtaa ccaccacgac cgccaccgcc accacgattc tcgcgtggct tggcttcgtt
     2341 aactgtcaga ctgcgaccgt tgacttcctt accatggaac tgagcaatag cagcttcgcc
     2401 ttcttccttg ctggccattt ccacgaagcc gaagccacgt gaacgaccag tgtcgcggtc
     2461 ttccacgacg ctcgcggatt cgactgtgcc ggctgccgca aaaagctcct gcagatcctg
     2521 gctggaggtc gaaaacgaga gatttcctac ataaagtttc attgtcatta gatgtacctg
     2581 tccctaaagg gaataaagag aagctgcgaa tcggatttgc ttcggtcgaa cggtaggagc
     2641 gaagggccgg ttttcgaatg ctctatggag cggcttctga agttgaaatc agcctgctct
     2701 cgaaccatcc aaaacgccag cgccaataga ctttacgcgc ggggcgcatc accgtcgatg
     2761 cgcgagagaa ggattaaccg tgcgtcacta tgcactatta cgtgaaatct agcaagcgtg
     2821 ggcggcctgg ggattgctga ttgccgattg gtgactgccg gtttgataat agggtatctg
     2881 gttcttagaa tggcttcggg agcaaattgg ctaataaaat gccggttatt cttaaattca
     2941 cgataacact tctggccttg gcggcttgcc tgttggccag cgtcaacgcc cagtcgaagc
     3001 aacccaaaca gaaaaagcct gttaatgaac tggcccggct tcaggaggag ttcgttaacg
     3061 ccacaaaaga ctataaggcc agcctgggaa aactattggc cacatatgag cgcgatgtca
     3121 ctaaggccga agcccacctg acccaaacgc gagcgctctt tgatcagggt ctaatcgcca
     3181 aggcccaact tgcccagagc gaaactgccc ttgcggctgc caaagacaag gttacggaaa
     3241 cctcacgatt aatggtcaac gccgacactc aaatcgccca gacgctgctc gaagctcagg
     3301 ctgaggagca actcgccaga actcgactag ctaaaggtcg caccttgcgg acagcgtcct
     3361 acatccgtta tgccggcggc ggcgggtggt tgttgtcaga cgcctggaag gtgcagcgtt
     3421 ttttcctcga ctccttcaaa aaacaattac cgatcgccgt tttcggccag ggaacgattc
     3481 acgatcgctg gcgactcgat catcgaaact ccatggacat ttccctgcat ccagatggac
     3541 ccgaggggca ggcgcttctg aacttcctgc gcaacaatgg aattcccttc cttgctttcc
     3601 gcggtgcgat ccccggcaca gctacggggc ctcac
//
LOCUS       DASUEX010000522         3289 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1344262, whole genome shotgun sequence.
ACCESSION   DASUEX010000522 DASUEX010000000
VERSION     DASUEX010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3289
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1344262"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="VFV61_08750"
     CDS             complement(<1..484)
                     /locus_tag="VFV61_08750"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX4900607.1"
                     /translation="MQKYDDDDALRPLGLRPATAEDTSFLMSLFASTRSEELALLAPD
                     HNLIEAFITMQFNAQRRQYDMSYPHANHSIVLWNEAPIGRLLVDKGDREFTLVDVALL
                     ADYRNIGIGTHLMQNLLKEAATAREPVRLHVLATGAAMRLYERLGFSRIGGDSVYLEM
                     I"
     gene            542..1222
                     /locus_tag="VFV61_08755"
     CDS             542..1222
                     /locus_tag="VFV61_08755"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEX4900608.1"
                     /translation="MSDNIKPFLNLSSAGKELATKLLANGAKGDIIVLGIVTAGVPVA
                     VEVARTLKAPLDLVLIRRLLAPGGPGTQACAVNVAGHLVVDEHIPPRPEKPETPFDSF
                     MEDALNAITLRAEVCRGNRPPFELEGRSILLVDCGIRTSLTMQAAIGAIRTLYPKSIT
                     AATPVTSSEGCTVVEALADQFIYLAAPEPFGNAGMWYRDFSRPGDEAIGQLLASFAGQ
                     IQGASGSA"
     gene            complement(1267..>3289)
                     /locus_tag="VFV61_08760"
     CDS             complement(1267..>3289)
                     /locus_tag="VFV61_08760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900609.1"
                     /translation="VQGSVPGADANTTLNLKLGGAGTEQNNFVAGDPFDFNDVFVFQG
                     ATPEGVFTLTRGNSLSSDPATVIMNNNVSPLSVFADGGQTVVMAAPPLPAAINETCTP
                     PAAPSLLANTETVDDKWAESAAANGGDVSQTLRAAHGERANKETALNLSATELELMRA
                     EAIRRWADFGLSATDLSRMQAISAQITDLPDSELTRINKGVIEIDGKAAGFGWYFDPT
                     PGDDSEFAVPVPNKESQATELSAAQGHVDLITVLMRQLRVALGRKRISLSGTQGWLMQ
                     NTLSVGTRRAPVLRAAAHSEVAPNVPQQAEVIQTDEQTQPAFDDSASQRRFRQVAKST
                     SSATSPGTLADVMLNIGILPAGETLTIRFRVTVDDPFTAIPPQVSNQGTVSGNNFGSV
                     LTDDPDVGGAADPTVTAIQVATVTPSVSVAVSPASTAEGGANLAYTFTRTGSTASALS
                     VDFSVGGTASFPGDYSQTGAAMFTPPTGTVTFGAGNTTATVDVTPLTDSEVEGAETVV
                     FTVTSGTGYIVGMPGTATGTITDSASDSTPPVITLHTTEIQLWPPNHRYQNFAVSDFV
                     LSASDSFDASVNLSSVYILNITSDELVKGEGDGNTFKDIVIGADCKSAQVRSERSSSG
                     DGRVYTITFKVRDASGNSTTATARVVVPIAHGNSAIDSGPKYTVSSSCP"
BASE COUNT          723 a          894 c          911 g          761 t
ORIGIN      
        1 agatcatttc caggtagact gaatcaccac cgattcggga aaacccgaga cgttcataca
       61 ggcgcatcgc ggcgccggta gccaacacat gaagtcggac cggttccctg gcggtggctg
      121 cttcctttaa tagattttgc atcaggtggg ttccaatgcc aatgttccga taatccgcca
      181 acagggcgac gtcaaccaaa gtgaattcgc ggtcgccttt gtcaacgagc aacctgccga
      241 taggcgcctc gttccataaa acaatgctat ggttcgcgtg cgggtagctc atatcatact
      301 gtcggcgctg cgcgttaaac tgcatcgtaa taaaagcctc tattagattg tggtcagggg
      361 cgagcagagc aagttcctct gagcgcgtgg aagcgaacag actcataaga aatgacgtat
      421 cttcggcggt tgccggccgc aatccgaggg gacggagagc atcgtcatca tcgtattttt
      481 gcatttccag atgtttatac tatgtaagat cttaatcaag tcaagaggat gaacaatcgg
      541 catgagcgac aacatcaaac catttttaaa tcttagcagc gccggaaagg aactcgcgac
      601 gaagttgctg gctaacggcg cgaaaggcga catcatcgta ctgggaattg taactgcggg
      661 cgtacccgtg gcagttgaag tggccaggac tcttaaagcg cctcttgatc ttgttcttat
      721 tcgccgctta cttgccccag gaggtcctgg gactcaggcg tgtgcggtga atgttgcagg
      781 tcaccttgta gtcgacgagc acataccacc gcgaccagaa aaacctgaaa cgccgttcga
      841 cagttttatg gaagatgcgc ttaacgcgat aacgctgcgg gccgaagttt gccgaggcaa
      901 tcgtcctccc tttgagcttg agggaagatc gattcttctc gtggattgtg gaattcgaac
      961 tagtctgacg atgcaagccg cgatcggagc cattcgcact ctttatccaa aatctattac
     1021 ggctgcgact ccggttacgt catcggaggg gtgcaccgtc gtcgaagcac ttgcagatca
     1081 gtttatctat ctggcagcgc cagaaccgtt tggtaacgca ggaatgtggt accgagactt
     1141 cagcagaccc ggtgatgaag ctattggcca actcttagcc agctttgcag ggcaaattca
     1201 gggcgccagc ggttccgctt gacagtcaga gctcaccgat aactctcgat cccttgttag
     1261 ctgagcctac ggacaactgc tgctgaccgt gtacttcggc ccgctgtcta tcgcggagtt
     1321 gccgtgcgct atcggtacaa ctaccctggc ggttgccgtc gttgaattgc cgctggcatc
     1381 tctgaccttg aaagtaattg tgtacacccg gccgtcacca ctgcttgatc gttcggagcg
     1441 tacttgagcg ctcttacaat cggccccgat cacaatatct ttgaaagtgt ttccgtctcc
     1501 ctctcccttc accagctcat cactagtgat attgaggatg tagacgctgc tgaggttcac
     1561 gcttgcatcg aacgaatcgc tggcgctaag aacaaaatcg gaaacggcga agttctggta
     1621 acgatggttc ggcggccaca gctggatttc ggtcgtgtgg agtgtaatga ccggtggcgt
     1681 tgaatcagag gcactgtctg taattgtacc ggtagccgta cctggcatgc cgacaatgta
     1741 accagttcca gacgtcaccg tgaagactac cgtttcggcg ccctcgacct cggagtcagt
     1801 aagcggcgta acgtctaccg tggctgttgt gttgcccgcg ccgaaggtca cggtgccagt
     1861 tggcggcgtg aacatagctg caccggtttg cgaatagtcg ccggggaagc tagcggtacc
     1921 acccaccgag aaatccacgc taagcgccga agctgtcgag cccgtgcgag tgaacgtgta
     1981 agcgaggttt gcgccgccct cggcagtaga tgccggcgag actgcaactg acacactcgg
     2041 agtgactgtg gcgacttgaa tcgccgtgac ggtcggatcc gctgcaccac caacatcagg
     2101 gtcgtcggta agcaccgatc caaaattgtt gccggacact gtgccctggt tcgagacctg
     2161 aggcggtatt gcagtgaacg gatcgtccac cgtaactcgg aacctgatcg tgagggtctc
     2221 gcccgccggc aatatgccga tattcaacat gacgtcagcc aacgtccccg gggaggtcgc
     2281 acttgatgta gatttcgcga cttgtctgaa ccttcgctga gaagcggagt cgtcaaacgc
     2341 aggttgcgtt tgttcgtccg tctggatgac ttcagcttgt tgaggtacgt ttggtgcaac
     2401 ttcactatga gctgccgccc tgagcaccgg agcgcgccgg gtgccaacgc tcagcgtgtt
     2461 ctgcatcaac caaccttggg ttccgctgag acttattctc ttgcgtccca aagcaacacg
     2521 cagctgacgc atcagcacag taataagatc cacatgccct tgtgctgcgc ttaactcggt
     2581 tgcctggctt tccttgttag gaactggaac cgcaaactct gagtcatcac caggcgtcgg
     2641 gtcgaaatac cagccaaagc cagcggcttt gccatcaatt tcaattacgc ccttgttaat
     2701 tcttgtcagc tcactgtcag gtagatcagt gatctgcgct gagatcgctt gcatgcgaga
     2761 aaggtcggtg gccgaaagtc cgaagtcggc ccaacgtctg atcgcttcgg ccctcatcag
     2821 ctccaactcc gttgctgaca ggttcaaagc ggtctcctta ttcgcgcgtt caccgtgggc
     2881 ggcgcgcagc gtttggctca cgtcaccacc gtttgcggcg gccgactccg cccatttgtc
     2941 gtcgacggtt tccgtattcg cgagcagcga cggtgccgcc ggcggagtgc atgtttcatt
     3001 aatcgcagct ggtaaaggtg gagcggccat tacgaccgtt tgaccgccgt cagcaaacac
     3061 actcaacgga gatacgttgt tattcatgat caccgtggcg ggatcagatg atagggaatt
     3121 gccccgagtc agagtgaaga ctccttctgg agttgctccc tggaagacaa acacatcgtt
     3181 gaagtcgaac gggtcgccag cgacaaagtt gttttgttca gtcccagcgc cgcccaactt
     3241 tagattgagg gtggtgttcg catcggcgcc gggaacggag ccctgaaca
//
LOCUS       DASUEX010000523         3059 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1592578, whole genome shotgun sequence.
ACCESSION   DASUEX010000523 DASUEX010000000
VERSION     DASUEX010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3059
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1592578"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2015)
                     /locus_tag="VFV61_08765"
     CDS             complement(<1..2015)
                     /locus_tag="VFV61_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008063311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEX4900610.1"
                     /translation="MKNPTSVALDPVCGMSVSPEKAAGAFAYKDQTYYFCSTHCLQRF
                     RENPEGFLKKSTEPPTSQLVGIQRETKPVAAPVAQAYTCPMHPEVRQDKPGSCPKCGM
                     ALEPVTVIAPQQKIEYTCPMHPEVVRDAPGNCPICGMALEPRTITLADEENHELKDMR
                     RRFWISAVLTIPVFVIGMSDLIPGAPLQRLVPSAVLAWTQLILASPVVLWGGWPFFVR
                     AWHSLVNRSLNMFTLIGLGVGVAYGYSVVGILFPDIFPHSFRAHGGGVPVYFEAAAVI
                     TTLVLLGQVLELKARSQTSSALKALLGLAPKTARRVAPDGSEQDVPLAHVQVGDLLRV
                     RPGEKIPVDGVVVEGSSAVDESMVTGESIPVEKTASDPVIGATVNGTGSLIMKAERVG
                     SETLLSQIVQMVAEAQRSRAPIQKLADLVAAYFVPAVILIAIITFIVWGTMGPEPRMT
                     YAIINAVAVLIIACPCALGLATPMSIMVATGKGAQAGVLFKNAEAIEVLRDVDTLVVD
                     KTGTLTEGKPKLVTVAPAANVNERELLRFAASLERGSEHPLAAAIVSGAQERGIQVVN
                     TQSFESITGKGVKGQVEGHAIALGNRGLLDDLGIDTDQLASQAEKFRADGQTVMFVAI
                     DGKAAGLVGVADPIKESTPEVIKQLHGEKLRIVMITGDSRTTANAVAA"
     gene            complement(2033..>3059)
                     /locus_tag="VFV61_08770"
     CDS             complement(2033..>3059)
                     /locus_tag="VFV61_08770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900611.1"
                     /translation="GTLELRGMFSFEPFTFPPGGSPLLFQTGETYKGRPIIDAQHPHD
                     LFMELSASYTVPIGDRATWFTYFGYPGEPALGPTAFMHRASASENPSAALSHHLQDSS
                     HISFGVLTTGFTYRWFKLEGSIFNGREPDENRYNFDAHRWNSRSARLSFAPNRNWSMQ
                     ISHGFLRDPEIQEPGDVRRTTASISYNKPFARGNWASSVIWGRNHENHSGEIFHLNGY
                     VAESTVKFLDKNYLYTRLELVDKNALLRDDDRLLLGITDHHPSFRIGAYTFGGARDVW
                     TREKLTMAIGSDLTFYSKPSVLNPIYGNNPVSWRMFLRFRPGKMDMSSAHGTHGSMNG
                     AEMTPEQ"
BASE COUNT          698 a          896 c          802 g          663 t
ORIGIN      
        1 gccgccacgg cgttagccgt cgtgcggcta tcgcccgtga tcatcacgat gcgaagcttc
       61 tcgccgtgaa gttgcttgat aacttcagga gtactctcct ttatggggtc agcaacgcct
      121 accagccccg cggctttgcc atcgattgca acgaacatca cagtttggcc gtcagcgcgg
      181 aatttctcgg cctggctagc caactggtcg gtatcgattc ccagatcatc cagcagcccg
      241 cggtttccca gagcaattgc gtggccctcc acctgccctt tgactccttt gccagtgatc
      301 gattcgaacg attgggtatt cactacttgt atccctcgct cttgagcacc ggaaacaatg
      361 gctgctgcca aaggatgttc actgccccgt tcaaggctgg cagcgaagcg taataactcg
      421 cgctcgttca cattcgcggc tggggcaact gtgacaagtt taggtttccc ttccgtaaga
      481 gttcctgttt tgtccaccac cagggtgtcc acgtcacgca gcacctcgat tgcttccgcg
      541 ttcttgaata gcaccccagc ctgagcgcct ttgccggtgg ccaccataat tgacatcggg
      601 gtcgccaagc ccaaagcaca gggacaagca attatcaaaa ccgcaactgc gttgataatt
      661 gcgtaagtca tccttggctc tggtcccatc gtgccccaca cgatgaaggt gatgatggca
      721 atcaggatta cggccggaac gaaataggcg gcaaccaggt cggccaattt ttggatcgga
      781 gcgcggctgc gctgggcctc ggctaccatc tgcacgatct gggacaaaag cgtttcggaa
      841 ccgactcgct ccgctttcat gatcaggctt ccggtgccgt taacagtcgc gccaataaca
      901 gggtcacttg cggtcttctc aaccgggatc gattcgcccg tgaccatcga ctcgtccaca
      961 gcgctcgacc cttccacgac aacgccatca accggaatct tttctccggg tcgtacacgc
     1021 aagagatcac cgacctgcac gtgcgccagc ggtacatcct gctcactgcc gtcgggcgca
     1081 acgcgccggg ctgtcttcgg cgccagaccc agcagcgctt tgagcgccga actggtctgg
     1141 ctgcgcgctt tcaattccag cacctggccc aatagcacaa gcgtggttat tactgcggcg
     1201 gcttcaaagt agaccggaac gccgccaccg tgcgcgcgaa acgaatgcgg aaagatatct
     1261 ggaaacagta tgccgacaac gctatagcca taagccactc ccacgccgag cccgatcagc
     1321 gtgaacatgt tcagactacg gttgaccagc gaatgccagg cccgcacaaa aaagggccag
     1381 ccaccccata gaacgactgg cgaagccaga atcaattgcg tccacgccag aaccgctgac
     1441 ggcacgaggc gctgcaaggg cgcgcccggg ataaggtcac tcattccaat tacgaagacc
     1501 gggattgtaa gcacggcgct aatccaaaac cgtcgccgca tgtccttgag ttcgtgattt
     1561 tcttcgtcag cgagagtgat ggtgcgcggc tcgagcgcca taccgcagat cggacagttg
     1621 ccaggggcat cacgaacgac ctccggatgc atgggacacg tgtactcaat tttttgctgc
     1681 ggcgcgatga cagtaactgg ctccagcgcc atgccgcact tgggacagga ccccggcttg
     1741 tcctgccgca cttcaggatg catcggacac gtgtaagcct gagctactgg tgccgcaaca
     1801 ggtttcgttt cacgctgaat tccaaccagc tgcgatgtcg gaggttcggt cgacttcttg
     1861 aggaaccctt ctggattctc tcggaatctc tgcagacagt gagtgctaca gaagtaataa
     1921 gtctggtcct tgtatgcaaa agcccctgcc gccttctcgg gacttacgga catcccgcac
     1981 acaggatcca aagcaacact tgtcggattt ttcatcgttg ctcctgccaa acctactgtt
     2041 caggcgtcat ctccgctcca ttcatgctcc cgtgcgttcc atgtgcgcta ctcatatcca
     2101 tcttgcctgg tcgaaagcgt agaaacatcc tccaggagac agggttattg ccgtaaatgg
     2161 gattgagaac agagggcttt gaatagaacg tcaagtcgct gccaattgcc atcgtcaact
     2221 tttcccgagt ccaaacgtcg cgagcgccgc cgaaggtata cgcgccgatg cggaaggaag
     2281 gatggtggtc ggtaattccc agtagcagcc ggtcgtcatc tcgaagcagt gcattcttat
     2341 ctaccaattc cagccttgta tagagatagt tcttgtcgag aaattttacc gtcgactccg
     2401 cgacgtaacc gtttaggtgg aagatctcac cgctatggtt ctcgtgattg cgaccccaaa
     2461 taacgctgct cgcccaattg ccgcgcgcga atggcttgtt ataactgatc gacgccgtgg
     2521 tgcgcctcac gtcgcccggc tcctgtatct ccggatccct taagaagcca tgactaatct
     2581 gcatcgacca gttgcggttg ggcgcaaaag acaagcgggc cgaacgagaa ttccagcgat
     2641 gggcgtcgaa gttgtagcgg ttttcatccg gctcacgtcc gttaaagatt gaaccttcta
     2701 acttaaacca gcggtatgta aatccagtcg tcaagactcc gaaactgatg tgcgatgaat
     2761 cctgcaaatg atgcgaaaga gctgctgagg gattttcaga agctgacgcg cgatgcataa
     2821 aagcggtagg acctaaggcc ggctcgcctg gataaccaaa ataagtaaac caggtcgcgc
     2881 gatctccaat aggcaccgtg taagaagcgg aaagctccat gaagagatcg tgcggatgtt
     2941 gcgcgtcaat gatcggacgg cctttgtacg tttcaccggt ttggaataaa agtggcgacc
     3001 ctcccggcgg aaaagtaaaa ggctcaaagc tgaacatacc gcgtaactcc aacgttccc
//
LOCUS       DASUEX010000524         2458 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1008288, whole genome shotgun sequence.
ACCESSION   DASUEX010000524 DASUEX010000000
VERSION     DASUEX010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1008288"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1300)
                     /locus_tag="VFV61_08775"
     CDS             complement(<1..1300)
                     /locus_tag="VFV61_08775"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEX4900612.1"
                     /translation="MATELKRFIIGRALRTEQAAHERLSKKTALAVFSSDALSSTAYA
                     TEEILLVLAVAAAFGQTGAFKYVIPISIGIAVLLVIVAISYRQTIHAYPSGGGAYIVA
                     KENLGTNAGLVAGASLLVDYVLTVAVSIAAGVAAITSMMQGTRYAWLDQHKVFLCLIF
                     ITFIAIANLRGVRESGALFAAPTYAFLISFLVMIAYGLYSYYTYGGAAPIPGPEEIKT
                     AEGYRLQPLTLFLILGAFSNGCAALTGIEAISNGVPAFKKPEARNAATTLVIMAGLLT
                     TMFLGTSVLAYLYGVHPHESETVISQFARIVFTGPVAWFYYVVQIATALILVLAANTS
                     FADFPRLSSLLARDRFLPRQFATRGDKLVFSNGIVILAIFASILVIAFGGDTSRLIPL
                     YAVGVFLSFTLSQTGMVRHWLKERAQSSKKNGKARKQREED"
     gene            complement(1355..2104)
                     /locus_tag="VFV61_08780"
     CDS             complement(1355..2104)
                     /locus_tag="VFV61_08780"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX4900613.1"
                     /translation="MSKRVLIIDDEENIRRVTRLTLQAAGYEIGEASDGARGLEAFGD
                     GSGWDAVLLDQRMPGIDGLETLRLINERDPTARVIMATAYASIELAVDAMKLGATDFV
                     RKPMTPEILRNAVAAALSKQPRLSTAWAPESPESSKEPLIQIITMNGFTILDSESARQ
                     EPYERRFRVKSPSGSEHEVLVQIDEEAVGYVERMTKRQLPPDNSFWTSQAQRLLGDYL
                     WQEGKVPPTRRLTIKDVDRDELPVAARWHTP"
     gene            complement(2108..>2458)
                     /locus_tag="VFV61_08785"
     CDS             complement(2108..>2458)
                     /locus_tag="VFV61_08785"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX4900614.1"
                     /translation="IERVIGNLVINALRHTKSGEIRISAQQRDNQVAISVGDTGSGIP
                     TEYLSHIFDKFVQVPDAPTGGVGLGLPISKSIVEAHGGQISVQSQVGCGTTFTFTLQL
                     SGVSASTAGKQREV"
BASE COUNT          561 a          661 c          667 g          569 t
ORIGIN      
        1 tgtcttcctc acgctgctta cgcgctttac cgttcttctt cgatgattgc gcacgctctt
       61 tgagccaatg ccggaccatc ccggtctgcg agagtgtgaa agagaggaaa acgccaactg
      121 catagagcgg aatcagcctg ctggtgtcgc caccgaaggc gattaccaga atgctggcga
      181 aaattgccag gattacaatg ccatttgaga agaccagctt gtctccgcgc gtagcaaatt
      241 ggcggggcag aaaacgatcg cgagccagca gactcgagag ccgcgggaag tcggcaaatg
      301 acgtgttagc tgcaagcacg aggattaatg ctgtcgcgat ctgcacgacg tagtaaaacc
      361 acgccactgg ccccgtaaac acgattcggg caaattgtga aatgacggtt tcgctctcat
      421 gcggatggac tccgtagagg taagcaagca cactggttcc cagaaacatc gttgtcaaaa
      481 gcccagccat gatcacgagt gtagtcgccg cgttccgcgc ctccggtttc ttgaacgctg
      541 gaacaccgtt agaaatggct tcgatacctg tcagtgccgc gcaaccgttt gagaaggctc
      601 ccagaatcag gaagagagtt aagggttgca acctgtaacc ttcggcagtc ttgatctctt
      661 ccggaccagg tattggcgca gcacctccat acgtgtagta gctataaagc ccataagcaa
      721 tcataacgag aaagctgatc aggaaggcgt aggttggcgc cgcaaacagc gcccccgatt
      781 cgcgcacacc acgcagattt gcaattgcaa taaaggttat gaagatcaag caaagaaaaa
      841 ccttgtgctg atccagccag gcatatcgag tgccctgcat cattgaagtg atggccgcga
      901 caccggcggc gattgaaacg gcaactgtga gcacatagtc aaccagcagc gaagcgccgg
      961 cgaccaagcc ggcatttgtg ccgaggttct ccttggcaac aatgtaggcg ccaccgcctg
     1021 atgggtaggc atggattgtt tgccggtagc tgattgctac gataacgagg agcacggcaa
     1081 tgccgataga aatcggtatg acgtacttaa acgcgcctgt ctggccaaaa gctgcggcaa
     1141 cagcgagtac gagtaggatt tcctcagtgg cgtaagcggt cgaagacaga gcgtcggaag
     1201 agaagacggc taaggccgtt ttcttggaga gtcgctcgtg agctgcttgt tccgttctca
     1261 gcgcgcgacc aatgataaaa cgcttgagtt ctgttgccac tactcttgtt cctcctggac
     1321 tagcttcaaa actccaagtc tcatcgcgct gtggctaagg agtgtgccaa cgagctgcga
     1381 cgggtagttc gtcacggtcg acgtctttga tcgtgagcct tctggttggg ggaactttgc
     1441 cttcctgcca caggtaatct cctaacaagc gctgggcctg gctggtccaa aacgagttgt
     1501 cgggtgggag ttgccgtttt gtcatgcgct ctacgtaccc gactgcttcc tcatcaattt
     1561 gcactagcac ctcatgctca ctgccactgg gactcttcac cctgaagcgc cgttcgtagg
     1621 gttcttgtcg cgcactttcc gaatccagga ttgtgaaacc gttcatggtg attatttgaa
     1681 tgaggggctc ctttgaactc tcgggtgact caggtgccca agccgtcgat agacgtggtt
     1741 gttttgaaag ggctgcggcc acggcattac gcaggatttc cggagtcata ggcttgcgca
     1801 cgaagtcagt agcgccgagc ttcatcgcat ccacagcgag ctcaattgag gcgtatgctg
     1861 ttgccatgat gacgcgggca gttgggtcgc gttcgttaat gaggcgcagc gtttccagac
     1921 cgtcgatgcc gggcattctc tgatccaata gcacggcatc ccagcctgat ccgtcgccga
     1981 aagcttccaa gccacgcgca ccgtcgctcg cctcaccaat ctcgtaaccc gccgcctgta
     2041 gcgttagtcg cgttacacgc cgaatattct cttcatcatc gatgatcaaa acgcgcttgc
     2101 tcattggcta cacttccctt tgtttccctg ctgtggacgc cgacactcct gatagctgca
     2161 aagtgaaagt gaaagttgtt ccgcacccaa cttgtgattg cacgctgatc tgcccgccat
     2221 gagcttcgac aattgattta gagatcggga gtcctaagcc cactcctccc gtgggtgcgt
     2281 caggaacttg cacaaactta tcgaagatgt gtgacagata ttcggttggg atgccgctgc
     2341 ccgtatcgcc gacagaaata gcgacctgat tgtcgcgctg ctgagcactg attctgattt
     2401 caccgctttt tgtatgtcgt agggcattga tgacaaggtt gccgattacc cgttctat
//
LOCUS       DASUEX010000525         4209 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1592856, whole genome shotgun sequence.
ACCESSION   DASUEX010000525 DASUEX010000000
VERSION     DASUEX010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4209
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1592856"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..861)
                     /locus_tag="VFV61_08790"
     CDS             complement(<1..861)
                     /locus_tag="VFV61_08790"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEX4900615.1"
                     /translation="MKNFRFILAVLTVCFSISSAFAQSNSGSLVGTVVDQNGSAIAGA
                     TVEITDDATKKTRTVLASDEGTFTIPQLDVGQYTVKVTAQGFKTYSATELKIDVGKQY
                     SLNVVLETGGLQESITVVAGADVINATNAELSNTVSPRQIQELPLNGRNPLALIALQA
                     GTSANGATNTSINGQRTSFTNITRDGINIQDNFIRANATDFAPQRPGVDDISEFTLTS
                     SNADASAGYGASQVQVATERGQSSFHGALFEYNRNSKFSSRPFFNNERAFLNRNQYGG
                     KIGGPVPLPRF"
     gene            complement(1265..1900)
                     /locus_tag="VFV61_08795"
     CDS             complement(1265..1900)
                     /locus_tag="VFV61_08795"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX4900616.1"
                     /translation="MNETTIHSPIGIAPMTATSRDLPSSNPTAKRTLDVGCGQNKYPG
                     AIGIDSNPRTQADVIHDLGAVPYPFTDNEFDEIICRHVIEHVPDVMGFVTELHRVTKP
                     GGRIKIVTPHYSNPDWATDPTHRNHFNSYSFTCFMPERTPFPFYTTVELKPLRTYVSL
                     ANLWRALGLELITNLDQHWPAFRFTRKFWEFYLSNICRGKELHFEFEVVKK"
     gene            complement(2000..3370)
                     /locus_tag="VFV61_08800"
     CDS             complement(2000..3370)
                     /locus_tag="VFV61_08800"
                     /inference="COORDINATES: protein motif:HMM:NF016354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HEX4900617.1"
                     /translation="MYEFAVTPAVTSIRRRGAEITEVAPSGIGAELELAPGDRITKVN
                     GRTIRDYLDFRFQTGGETELTIEVRKSSGEDWELQIERAEDEEFGFSFEQIVPRQCAN
                     ECIFCFCKGNPEDARPSLFVRDEDIRLSFLYGNYTTLTSITEDEMSRIIEQRLSPQYV
                     SVHATDLDVRAYLLGVDKKRADISTNLERLLAAGIEIHAQVVLCPGINDGEILRRTIN
                     DLAADYPRITSVAIVPLALTRYNTDERLTPVTPDFCRSVISEVTQLQKELRQRLGTTF
                     AFLGDEIYLRAGHNIPGRSHYGNYPQIEDGIGMVRAFESDFAKLLKRIERELSARSEM
                     RRSTILTGTMFAPILEELIEKLNKQSGTQIQVVGVENNYFGGDVSVAGLLTGGDLVAA
                     RERISGDFAIIPKTMLKSDEEIFLDGMSLAQLDERLGLPVYPVDLDSFAAFLFTPGFS
                     PVSSVI"
     gene            3744..>4209
                     /locus_tag="VFV61_08805"
     CDS             3744..>4209
                     /locus_tag="VFV61_08805"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX4900618.1"
                     /translation="MKLKSPVASASLVLLSLAVPLSATAQSMSAAPVREEVAQQAIAS
                     ADISKNRDVTASEGEKQYDSGVSLYDSAKFSEALVAFKEATKVRPNDAQAYFMLGMSE
                     AKLQLYKDAVESFRRATRLKPDWPEAQFRLGVVSHVWGRSALATEAYAMLVKL"
BASE COUNT          977 a         1090 c         1122 g         1020 t
ORIGIN      
        1 gaagcgcggc aacggaactg gcccgccgat cttgccgcca tactggttgc ggtttagaaa
       61 cgctctttcg ttattaaaga acggtcgcga agaaaacttg gagttgcggt tgtattcgaa
      121 aagggcgcca tggaacgaac tctgaccgcg ctcggtcgcc acctgcacct gcgaggctcc
      181 atagcccgcc gaagcatctg cgttagaaga cgtgagggtg aactcactaa tgtcgtctac
      241 gcccggacgt tgtggcgcga aatccgtggc gttggcacgg atgaagttgt cctgaatatt
      301 gatgccatcg cgcgtgatgt tggtgaaaga cgtgcgctgg ccattgatcg aggtattagt
      361 cgctccattg gctgaggtgc ccgcctgaag ggcgattaga gcaagaggat ttcgtccgtt
      421 aaggggcagt tcctggatct gcctggggct taccgtattg ctcaactcag cgttggtggc
      481 attgattaca tctgctccgg ccacaacggt aatgctttct tgtaagccac ccgtttccag
      541 aactacattc aaagaatatt gcttgccgac gtcaattttc aattccgtgg ctgaataggt
      601 cttgaaaccc tgcgccgtga ccttaactgt atattgacct acatcgagct ggggtatggt
      661 gaaggtgcct tcatcactgg ctagcactgt tctggttttc ttcgtggcgt cgtctgtgat
      721 ctcaacagtt gctccggcaa ttgcgctacc gttttggtca acaacggttc cgaccaaact
      781 gccactattg ctttgggcga atgctgacga aatggagaaa caaacggtca gcaccgccaa
      841 aatgaatctg aagttcttca tcggtaggct ccttggaaga ctaagttggc ttgtttgaac
      901 ttcggcaagt acctccgctt tatttggggc gcgttgccga gaatgcggaa atttcatttt
      961 gggttaggcg ccggcaggag gaaaacacta ccgaacaaga attttgcaat aatcatgcct
     1021 tccgaacgct gaactggaaa ctgttgttat ttgagattta tggcccacag aagtaacctg
     1081 gactgccaaa atcttgcaaa atttggattc gcatacgaat acttagatcg actgcggtgt
     1141 aaggccaaac ccaaagcatg tcagttttgc tttgcagcct gtttatcgga gaggtgtgtg
     1201 cgggagcgct ggtgagtggg aatctacgat aactgcctac tgactaccat ctaccatcta
     1261 ccatctactt cttcaccact tcaaactcaa aatggagttc tttgccgcgg cagatgttgc
     1321 tgaggtaaaa ctcccagaac ttgcgggtga agcgaaacgc cggccagtgc tgatccaggt
     1381 ttgtaattag ttccagaccc agggcgcgcc agagattcgc cagactgacg taggtacgca
     1441 gcggtttcaa ttccacagtt gtgtagaagg gaaagggcgt tcgttccggc atgaagcagg
     1501 taaacgagta gctattgaag tggttgcgat gggtggggtc cgtggcccaa tcaggattcg
     1561 agtagtgtgg cgttacgatc ttgatgcggc cgcctggttt ggtaacacga tgcagttccg
     1621 tgacgaagcc catcacatcg ggaacgtgtt cgatcacatg acgacaaatg atctcgtcaa
     1681 attcgttgtc cgtaaaagga taaggaacgg cgcccagatc gtgaattaca tccgcttgag
     1741 tccgcggatt cgagtcaata ccgatagccc cgggatattt gttctggccg cagccgacgt
     1801 cgagagttct tttggcggtt gggtttgagc ttggcaggtc tcgtgacgtg gcagtcatcg
     1861 gagcgatacc gatcggtgaa tgtatggtgg tctcattcac accgggcttt cgcccggtga
     1921 aaacaagcca ggcgctgtaa ccgtctcaac ggtttgctga ctcctcttga aagaaaccgt
     1981 taagtcggtt cctctttcct tatatgacgc tgctcaccgg gctaaagccc ggtgtgaata
     2041 agaaagctgc aaacgaatcc aggtccacag gatagactgg caatcctaat ctctcgtcca
     2101 actgcgccag cgacatcccg tcaagaaaaa tctcctcgtc gctcttgagc atagtcttcg
     2161 gaatgatggc aaaatcaccg ctaatccgtt cgcgggcggc aaccaagtcg ccccctgtga
     2221 gcaagcccgc tacagagacg tcgccaccaa agtaattgtt ctcaactccc acaacctgga
     2281 tctgagttcc gctttgctta ttgagctttt cgattagctc ttcaagaatt ggcgcgaaca
     2341 tcgttccggt gaggatagtg ctgcggcgca tttccgatcg agcggatagt tcacgttcga
     2401 tccgcttcaa cagtttcgca aagtcgcttt cgaaagcccg taccatcccg ataccgtctt
     2461 caatctgcgg atagtttcca tagtgactgc gcccggggat gttatgtccg gctcgtaaat
     2521 agatttcgtc gcccaaaaaa gcaaacgtag ttcccaaccg ttggcgcagt tccttttgca
     2581 actgcgttac ttcactgatc acgctgcggc aaaagtcggg agtgaccgga gtgagacgct
     2641 cgtctgtgtt gtagcgcgtc aatgcgagcg gcacgatcgc gacgctggtg atgcgcggat
     2701 aatctgcagc gagatcattg atggtgcgtc tgagaatctc gccatcgttg atccctgggc
     2761 aaagcacgac ctgggcgtgg atctcaatgc cggcagcaag caaacgctca aggttcgtgg
     2821 aaatgtctgc gcgcttttta tcaacgccaa gcaggtatgc gcggacgtca agatcagtgg
     2881 catgaacaga aacgtactgc ggcgacagac gctgctcaat gatgcgactc atttcgtctt
     2941 cagtgatcga agtcaacgtc gtgtagttgc cgtagagaaa ggaaaggcga atgtcttcgt
     3001 cgcggacgaa gagcgaaggc cgcgcatcct caggattgcc tttgcaaaaa cagaagatgc
     3061 attcgttggc acattgccgg ggaacgatct gttcaaaact aaaaccaaac tcttcgtctt
     3121 cagcgcgttc gatctggagt tcccagtctt cgccgctgct tttgcggact tcaatcgtta
     3181 gttcagtttc accgccagtt tggaagcgaa aatccaggta gtcccgaatg gtgcgtccat
     3241 tcaccttcgt gatgcgatcg ccaggcgcga gctccagctc agcgccaata cctgagggcg
     3301 caacttcggt gatctcggcg ccgcgacgcc ggatactcgt gaccgctggt gtaactgcaa
     3361 attcgtacat aagtaagagg tagacagtag gcagtaggca gtagagaaaa ctacatgctc
     3421 tgactcccta aaactgacga agaacctcgg ctatttacca ccaactactg attgttaccc
     3481 gcaggctacc gtctactgtc tattgtctac tgcgctctga ctataacaaa ggccctctta
     3541 ttttgctgaa gagcgccagc aaagcgctct ttaattgggg tttttctgcc attcggcctt
     3601 tataagtgag gagtcgcctt atgatttccg gcttcagact cctgagatcc ttgaggtata
     3661 ctgcggcgca acagctccgc caggcatttc cccaagtcgc ggttgattcc gcgaaacgaa
     3721 actaaccgct ggcatttcaa gctatgaaac ttaaatcccc cgtcgcttcc gcctccctgg
     3781 ttttgttgtc gctcgctgtt cctctatcag caacagctca gtcaatgtcg gctgcgccgg
     3841 ttagggagga ggtcgctcag caagcaatcg catccgccga catctctaaa aacagagacg
     3901 tcaccgcctc tgagggagag aaacaatacg actccggagt cagtctgtac gactccgcga
     3961 agttcagcga agctcttgtc gctttcaaag aagcaacgaa ggtccggccc aacgacgcgc
     4021 aggcttattt catgctggga atgtctgagg cgaagttgca gctttataag gacgccgtcg
     4081 aatcgttcag acgcgccacg cgcctcaagc ccgactggcc cgaagcccaa ttccggcttg
     4141 gtgtcgtctc gcacgtgtgg ggccgcagcg cgctcgcaac cgaagcctat gcgatgctgg
     4201 taaagctga
//
LOCUS       DASUEX010000526         2605 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1592929, whole genome shotgun sequence.
ACCESSION   DASUEX010000526 DASUEX010000000
VERSION     DASUEX010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1592929"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /gene="def"
                     /locus_tag="VFV61_08810"
     CDS             complement(<1..418)
                     /gene="def"
                     /locus_tag="VFV61_08810"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF013492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEX4900619.1"
                     /translation="MKGLGDKTMAVMEVLTWPNPVLDTPGDPVTEFDAKLKKLVGNMF
                     ETMYAAPGVGLAAVQVGVPKRLFVMDCSGGKDPDQRIVMINPEVLRVEGTQNGDEGCL
                     SFPGIFFPVERGLRAIVRAHDLDGNEFEVDGMELTAR"
     gene            complement(552..1721)
                     /gene="aroC"
                     /locus_tag="VFV61_08815"
     CDS             complement(552..1721)
                     /gene="aroC"
                     /locus_tag="VFV61_08815"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEX4900620.1"
                     /translation="MFRFTTAGESHGRALVAILEGLPAGMPVESEQINADLKRRQAGY
                     GRGGRMKIEQDQVQILSGVRHGLTLGSPIALLIENKDWANWTEVMSVAPTEVQPEKLR
                     QLKRPRPGHADLAGGQKFGARDLRNVLERASARETAARVACGALARQLLSGFGVEILS
                     HVTQLGGIPEKPLQRSWEEIASISSDAPLRCADAEAERRMVELIDAKREAGDTLGGVF
                     EVVARGVIPGLGSHTAWDQKLDGRFAQAIMSIPAVKAVSIGSGVEASSLPGSEVHDEI
                     AYNRETSEFIRESNRAGGLEGGITNGEELRISGHLKPLSTLRRSLRSVDIDSKEDRPA
                     GFERSDITAVPAAGVIGEAMVALVLAAAMREKFGGDSLGEMKRNFEGYREQLRGY"
     gene            complement(1740..>2605)
                     /locus_tag="VFV61_08820"
     CDS             complement(1740..>2605)
                     /locus_tag="VFV61_08820"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX4900621.1"
                     /translation="GVILEAEVRLLAKPSGFLSGVVFFDSEPQLLAFVAVARAASLET
                     RASHSNGSIDARALEYFDQESLGFLRQKYAAIPTQAIGAVFFEQETTVNTEEPLMTAW
                     LSLLESHQALVDESWFATSEQDQAKLREFRHQLPALMNEWFAHYQQRKVSTDMAVPDK
                     EFAGMLRFYADTLRSGSLRYTIFGHIGDNHVHVNILPRDDREAAQAREIYRRFVERAI
                     KVGGTISAEHGIGKLKRQYLKMLYGEQHLREMAALKRAFDPAGILGRGNIFPEEYLPE
                     VQGPKPNDPRF"
BASE COUNT          575 a          815 c          641 g          574 t
ORIGIN      
        1 aacgggctgt cagttccatt ccatcaactt caaactcgtt tccatcaaga tcgtgagcgc
       61 gaacaatcgc gcgcaggcct ctttcgaccg ggaaaaatat tccggggaac gagagacatc
      121 cctcatcccc attttgcgta ccttcaactc gtaacacttc cggattgatc atgacgatcc
      181 tctgatccgg atctttcccg ccagaacaat ccatcacgaa gagccgtttc ggcacaccaa
      241 cctgcaccgc cgccaggcct acgccgggag cggcgtacat ggtctcaaac atgttgccga
      301 ctaacttctt cagcttggcg tcaaattccg tgaccggatc gcccggcgta tcaagcaccg
      361 gattcggcca cgtaagtact tccattaccg ccatcgtctt atcgcctaaa cctttcacga
      421 actgcctacg atgtcgattt tacgacgaac gatgcttggc gcgccaccag gcaagctgag
      481 tcgattcttc tgcgccgcgt cggtgtgctt cgtggatcgc acaacagaag gggattcgag
      541 ttgagctggc ttcaataacc tcgtagttgt tctcgataac cttcgaagtt gcgtttcatc
      601 tctccaaggc tgtcgccgcc aaatttctcg cgcatagccg cagcaagcac cagggccacc
      661 attgcttctc caataactcc ggcagcaggt acggcagtaa tgtctgacct ctcaaaaccc
      721 gccggccgat cttccttact atcgatgtca acggaacgaa gggaccgccg caaggtagaa
      781 agcggtttga gatggccact aattcgcaat tcttcgccat tcgtgattcc gccttcgagt
      841 ccgccagcgc gattggattc gcggatgaat tcactcgtct cgcggttgta ggcaatctcg
      901 tcgtggactt cggaacccgg cagactcgat gcctcaactc ccgaaccgat cgataccgcc
      961 ttcacggccg gaatggacat gattgcctgt gcaaaccgtc catcgagttt ttgatcccag
     1021 gccgtgtgtg aaccgagacc gggaataacg cccctggcca ccacttcaaa tacgccaccc
     1081 agtgtgtcgc cggcttcgcg cttggcgtcg attagttcca ccatccgccg ttcggcttcc
     1141 gcgtcggcac agcgcaaagg cgcgtcactt gagatacttg cgatctcttc ccatgagcgt
     1201 tgcagaggtt tttcgggaat gccgccaagc tgtgtcacgt gactcaagat ctctactccg
     1261 aaaccgctca acaactgcct cgccaaagca ccacacgcca cacgcgccgc cgtctcccgg
     1321 gctgaggccc gttccaacac gttgcgcaga tcgcgggcgc cgaatttctg accgccagcc
     1381 aaatcagcat gtcctggtcg gggacgcttt aattgccgga gcttctcagg ctgaacctct
     1441 gttggggcga ccgacatcac ctcggtccaa ttggcccagt ccttattctc aatgagcagt
     1501 gcgataggtg agccgagggt gagcccgtgc cgaacaccac tcagaatctg aacctgatcc
     1561 tgctcaatct tcattcggcc cccgcggcca taacctgcct gcctccgctt aagatcggca
     1621 ttaatctgtt cgctctctac gggcatgccg gcaggcagcc cttcgagaat cgcgaccagg
     1681 gcgcgaccgt gggattctcc agcagtggtg aagcgaaaca tggttagtgg tcagttgttt
     1741 cagaaccttg ggtcattggg ctttgggcct tggacttcgg gtaggtactc ctccgggaaa
     1801 atgtttcccc gccccagaat ccccgccggg tcaaaggcac gcttcaaggc agccatctca
     1861 cgcaagtgct gctcaccata gagcatcttc aaatactgcc gcttcaattt cccgatccca
     1921 tgttcagcag agatcgtgcc tccaactttg atcgcacgct ccacaaagcg ccgatagatc
     1981 tcacgcgcct gtgcggcctc ccgatcatct cgaggcagga tgttcacgtg gacgtgattg
     2041 tcaccgatgt ggccgaagat cgtatacctg agactgcctg aacgaagcgt gtcggcataa
     2101 aacctaagca tgcccgcaaa ctctttatcg ggcacggcca tgtcggttga gaccttgcgc
     2161 tgttggtagt gcgcaaacca ttcattcatt agcgctggca actgatgccg aaactctcgt
     2221 agtttcgcct ggtcctgctc actggttgca aaccacgact catcaaccag cgcctgatgc
     2281 gattccagca aggacaacca ggcagtcatc aacggctctt cggtattgac agtggtctcc
     2341 tgctcaaaaa agactgcccc gatcgcctgt gtcgggatgg ccgcatactt ttgccgtaag
     2401 aacccgagcg actcctgatc gaaatactcc aaagcgcggg cgtcaataga tccgttggaa
     2461 tgtgacgccc tcgtttcgag tgaagcagcg cgcgcgacag ccacaaacgc gagcaactga
     2521 ggctcagaat caaaaaacac aactccactc aaaaatcctg atggtttcgc cagtaaacga
     2581 acctcggctt ccagtatcac cccca
//
LOCUS       DASUEX010000527         2757 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_94896, whole genome shotgun sequence.
ACCESSION   DASUEX010000527 DASUEX010000000
VERSION     DASUEX010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94896"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            99..884
                     /locus_tag="VFV61_08825"
     CDS             99..884
                     /locus_tag="VFV61_08825"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX4900622.1"
                     /translation="MRDRDNTAMYPKRARDLKAADLAVLIRQLDFGPTLDESAAAELA
                     RVSVVLDVRRRRFIYKAGDPADSLFAIVHGRVKLCRIDPPTEREAVIDILSEGSLFGE
                     SALYSTAGRRENCAITYANSRLIKIPAGEFQKGMVGNSQLHNYTFRLIGQRLENAERR
                     LADFALNAIPARLDRLLAEFSNRYGVPEREGVLIDIPLPHREIASIVGSTRESVTVRL
                     NAMRREGTIEFVNRRILVKRPESLVHEPVMTTEDSLVRELQPL"
     gene            1079..1687
                     /locus_tag="VFV61_08830"
     CDS             1079..1687
                     /locus_tag="VFV61_08830"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX4900623.1"
                     /translation="MFAPKACARRAAILVIPLIVLCLAAPGCSQRGANNSEPTPVLSE
                     PPGTTFPMPPVSNEGSNASAGWTLADGGRVSLAELRGRVVVLDMYATWCEPCRQSIPH
                     LISLHERYKDRGLSLVGLNVGGPDDRVKVANFAAEFRISYPLGFPDKSLTDLFLSDDS
                     SIPQTFVFSREGRLVKRYIGYDQSMPQQLEDLIKAELVSTSK"
     gene            1724..2494
                     /locus_tag="VFV61_08835"
     CDS             1724..2494
                     /locus_tag="VFV61_08835"
                     /inference="COORDINATES: protein motif:HMM:NF018446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HEX4900624.1"
                     /translation="MLREQLIKKETAGGTFRWRSHEISRIEGLSDAVFGFAITLLVVS
                     LEVPKTFNELLEAMHGFGAFAICFTLLFIVWYNQYKFFRRYGLKDTVTVVLNGALLFV
                     VLFYVYPLKFLFTAVVDRFTGGHGEVRLPNGNVEAMMEAGQVPMLMLIFGVGYLAVFS
                     VFVFLYLRAYKKRQELELSRLEIFDTISSVKEYSLHCSIAIISISLVLIGGPQMAGLA
                     GMIYMLTGVVMTLHGWRSRRRRKRLATRLADETVLESA"
     gene            2513..>2757
                     /locus_tag="VFV61_08840"
     CDS             2513..>2757
                     /locus_tag="VFV61_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900625.1"
                     /translation="MISVNLTLITFVVVMVCWFVFALTFLLRKKPAVVSDQKRDPGSI
                     PGVVLQGLSYLIVWAGRRPFSTSLVSFSKVAAVILEIL"
BASE COUNT          575 a          744 c          734 g          704 t
ORIGIN      
        1 gttttcctta ctcggtaccg ctacccaacg tcaggatcat cataatttat agttagaagc
       61 gtcttttggt cttcgagaag cctttaactc aatctcccat gagagacagg gacaacacag
      121 ccatgtaccc gaagcgtgcc cgcgatttga aagccgcgga tcttgcagtc ttaatccgcc
      181 aactcgattt tggtccgaca ctggacgagt cggctgcagc cgaactcgcg cgcgtttccg
      241 tcgttcttga tgtgcggcgc cggcgtttca tttacaaggc gggtgacccc gctgactccc
      301 tgtttgccat tgtccatggt cgcgtcaaac tctgtcgcat tgacccgcca acggaacgcg
      361 aagccgtaat tgacatcctt tcggagggct cgttgtttgg cgaatcagcc ctctactcca
      421 ctgccgggcg cagggagaat tgcgcgatta cttacgcaaa ttcgcgacta atcaagattc
      481 ctgcggggga gtttcaaaag gggatggtgg ggaacagtca acttcataat tacactttcc
      541 gcttgatcgg tcaacgactt gaaaatgccg agcgccggct tgccgacttt gccctgaatg
      601 cgattccagc ccgccttgat cggttgctcg ccgagttttc caaccgctac ggcgtgcctg
      661 agcgagaggg cgtattgatc gacattccct tgccgcatcg ggagattgcc tccatcgtgg
      721 gctccaccag ggagagtgtt acggtgcgct tgaatgcaat gcgccgggaa ggtacgatcg
      781 aattcgttaa cagacgcatc ctggtgaagc gacccgaatc actcgtgcac gagccggtaa
      841 tgactaccga agattcgttg gttcgcgaat tgcagcctct atgaagtgtc cgatttgcgg
      901 taagccggtt gagtggaaag acaatccgtt tcgccccttt tgttccgaac gttgcaagct
      961 gctggatctg ggccgttggg taagtgagga atacagcgtt cccggaccgc ctgttccctt
     1021 cgagtccgac gagactccgc ctcccccacc ttcccatgac gacgattgag cgccggctat
     1081 gtttgctcct aaagcctgcg cgcgacgcgc cgccatcttg gtcattccct taatagtgct
     1141 ttgcctggca gcacccgggt gctcgcaacg aggcgctaac aactccgaac ccacgccggt
     1201 actctccgaa cccccaggca cgacttttcc aatgccgccc gtaagcaacg aggggtctaa
     1261 cgcatctgct gggtggacgc ttgctgatgg cggacgtgtc agcttggcgg aactacgggg
     1321 cagggtcgtc gtcttggaca tgtatgccac gtggtgtgaa ccatgccggc agagcattcc
     1381 gcacttgatc agtttgcatg agcgttacaa ggacagaggg ctgtcactcg ttggtctgaa
     1441 cgtcggcggc ccagacgatc gcgtcaaggt tgcaaacttc gctgccgagt tccgcatttc
     1501 ctatccgctc ggctttccag acaaatcgtt aactgatcta ttcctgtcag acgacagcag
     1561 tattccccaa accttcgtat tcagtcgcga gggccggctc gttaaacgct atattggtta
     1621 tgatcagtca atgccccaac agttggaaga cttaatcaag gctgagttgg tttcaacctc
     1681 gaagtgactt gcagtttcct taacactatt tctggagcgg cgcatgttaa gagaacaact
     1741 cataaagaaa gaaaccgcag gcggtacttt tcgctggcgc agtcacgaga tctctcgtat
     1801 cgagggtttg agcgacgccg tgtttgggtt tgcgatcacc ctgctggttg tttccctcga
     1861 agtccccaag accttcaatg aacttcttga agccatgcat ggctttgggg ctttcgccat
     1921 ctgcttcacg cttcttttca tcgtctggta caaccagtac aaattctttc gtcgttacgg
     1981 attaaaagat accgttacgg tggtgctgaa tggcgccctg ctctttgtgg ttctctttta
     2041 cgtttacccg ctaaaatttc ttttcacggc agtcgtggat cggttcaccg gaggtcacgg
     2101 cgaggtccga ctgcccaacg gcaatgttga agcgatgatg gaagccggac aggtgcccat
     2161 gctgatgttg atttttggtg ttggttacct cgccgtgttt tccgtttttg tgtttcttta
     2221 tctgcgcgcg tacaaaaaac gtcaggaact cgaactaagc aggctcgaga tcttcgacac
     2281 gatcagcagc gtcaaggagt actcgctcca ctgtagtatt gccataatct caatttcgtt
     2341 ggtgctgatt ggaggcccgc aaatggccgg cctggcagga atgatctaca tgctcaccgg
     2401 cgtagtaatg actctgcatg gatggcgatc gcgcagacga aggaaacgtc tggcaacgag
     2461 gctcgccgat gaaacggtgc tggaatcagc ttaattcgac ggcgtctcaa gaatgatcag
     2521 cgtgaatctg acgttaatta cattcgtggt tgtgatggtg tgttggttcg tgttcgctct
     2581 gacattcttg ctgcgcaaaa aacccgcagt cgtgtccgac caaaaacgag atcccggctc
     2641 aattccggga gttgtgttac aaggactgtc ctatctgatt gtgtgggccg gccgccgtcc
     2701 ttttagtact tcgctcgttt ccttcagcaa ggtcgctgcc gtcatactag aaatcct
//
LOCUS       DASUEX010000528         2715 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_428671, whole genome shotgun sequence.
ACCESSION   DASUEX010000528 DASUEX010000000
VERSION     DASUEX010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428671"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..674
                     /locus_tag="VFV61_08845"
     CDS             <1..674
                     /locus_tag="VFV61_08845"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX4900626.1"
                     /translation="TLAETELLAIRGGLMGMVFQEDSLFTGLRVYENAAYRLEEHGWT
                     EEETKKAVADVLEFVGLQGEENKLPEELSGGMKRRLEIARALIGWPRIMLFDEPTMSL
                     DPIVAIQVLDLVIRARDINHVSSLYVTKKIYESPQLANFCASEAEGGKIVITRADPGS
                     LPKTRVIVLDNGRIAFSGTVNEFQRSDLPVIRELLALERHDHSKDIYFTDPWDKRRLP
                     KERLL"
     gene            complement(820..1383)
                     /locus_tag="VFV61_08850"
     CDS             complement(820..1383)
                     /locus_tag="VFV61_08850"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX4900627.1"
                     /translation="MLEKKDSETKRTELQKSEQTPAVRTPFTFMRRFAEDMDRMFEDF
                     EDFRLPRVFGREFFPFRMELKEVGWVPQIEVLQDKKHLTVRCDLPGMKRDDIKVEMKE
                     DVLTISGERNEEKKEEGEGFYRTERSYGSFYRQVPLPAGVKSDTAEATFHNGVLEIKM
                     VTTQTEPSTRKLEIKETAAKAVAKAAG"
     gene            complement(1472..2143)
                     /locus_tag="VFV61_08855"
     CDS             complement(1472..2143)
                     /locus_tag="VFV61_08855"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX4900628.1"
                     /translation="MERKKTDNEIKQQVLRELKWDSRIAWAQIGVEVREGVVTLSGAV
                     SSYAKKSAAQHAAHRVAGVLDVANDIEVKPTGAFARTDSDIAKAVRHTLEWDALVPDE
                     RIRSTVSDGWVTLEGDVDIWREREDAEKAVLKLAGVVGVLNMIRILPKAIDPKELREE
                     IEDALDRRAEREADRLRIEVKDGAVELWGRVHSWREKRAVLGSIGHMPGVLAVKEHLR
                     IDPYF"
     gene            complement(2186..2677)
                     /locus_tag="VFV61_08860"
     CDS             complement(2186..2677)
                     /locus_tag="VFV61_08860"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX4900629.1"
                     /translation="MKVKDIMTCEAQVCDLNASLAEAAKTMWEADCGVLPVLKDGKEI
                     VGLITDRDICMAVAMRDCNPAAISAEQVISGAVYSVTPEDDVHEALKIMGEHKVRRLP
                     VVSAEGELKGMLSMNDIVLKAQETKKGKMPELAYADVVNTYKTISAHPFAVSQAQAAG
                     TSP"
BASE COUNT          574 a          795 c          662 g          684 t
ORIGIN      
        1 ggacgcttgc ggaaaccgag ctgctggcaa tccgcggcgg gctgatgggt atggtctttc
       61 aggaagattc gctcttcacc ggactacgag tttacgagaa cgctgcctat agattggaag
      121 agcacggttg gacagaggaa gagacaaaga aggccgtcgc tgacgtgctg gagtttgttg
      181 gtcttcaagg tgaggaaaat aaactcccgg aagagctttc cggcggcatg aagcgccgcc
      241 tggagattgc gcgcgcccta atcggttggc ctcgcatcat gttattcgat gagccgacga
      301 tgagtctcga tccgattgtg gcgattcaag ttcttgacct cgtcatccgc gctcgcgaca
      361 ttaaccatgt ctcttccctc tacgtcacga agaagattta tgaaagtcct cagctcgcca
      421 acttctgcgc ctcggaggcc gagggtggaa aaattgtgat tacccgggca gatcccggct
      481 ctttaccgaa aacgagagtg attgttcttg ataacgggag gattgcattc agcggaacag
      541 ttaatgagtt tcaaagaagc gatctgccgg taatcaggga actacttgcc ctggagcgcc
      601 acgaccattc gaaggatatt tacttcaccg atccctggga caaacggcgc ctgcctaaag
      661 aacgacttct gtagaaaaag tggtgtcgac gcgtagcgtc ggcgtgaacg gttggaccaa
      721 agttcgatga ggtttagggc ccctggacca gtcgccggtg gatccgtgtt tgaacagatc
      781 cccccacgcc cgacgactgg cccacacggg ccggcgccac taacctgccg ccttagcaac
      841 agccttagcg gcggtctctt taatttcgag cttgcgagta gaaggttctg tctgcgtcgt
      901 aaccattttg atttcgagca cgccattgtg gaaggttgct tctgcagtgt cgctcttgac
      961 gccagcaggc agcggcacct gacgatagaa gctcccgtag ctacgctccg tgcgatagaa
     1021 cccttcgcct tcttcctttt tctcctcgtt gcgctctccg gatattgtca acacgtcttc
     1081 tttcatttcg actttgatgt cgtctctctt catcccgggc aagtcgcaac gcactgtgag
     1141 gtgtttctta tcctgaagaa cctcaatttg cggcacccac ccaacctctt tcaattccat
     1201 ccggaaagga aaaaactctc tgccgaagac tcgaggaaga cgaaagtctt cgaagtcttc
     1261 aaacattctg tccatgtctt ctgcgaaacg acgcatgaat gtaaacggag tccgcaccgc
     1321 aggtgtttgc tcactctttt gcagttcggt ccgcttcgtc tcgctgtctt ttttctctaa
     1381 catactgctc tccttctcag tgccgcgaat catttcgcag catcaataaa attcgttagt
     1441 ttcaattact acgttcgccc ctgcttcgga attagaagta gggatcaatg cgcagatgtt
     1501 ccttgacggc caacacacct ggcatgtggc cgattgaacc aagcaccgcc cgcttctccc
     1561 gccacgagtg aacacggccc caaagctcga cggcgccatc cttaacttcg attcgcagac
     1621 gatccgcttc tctctccgct cgacgatcga gggcgtcctc aatctcctcg cgcagttctt
     1681 tcggatcgat ggccttggga agaattcgga tcatattcaa aactccgacc accccagcca
     1741 acttcaagac agccttttcc gcatcctcac gctcgcgcca gatgtcgaca tctccttcga
     1801 gagtcaccca accatccgaa actgtagagc gaatgcgttc gtcagggacc agtgcatccc
     1861 actccagcgt atggcggacc gccttcgcta tatccgagtc agtgcgcgcg aaagcgccgg
     1921 tcggcttaac ctcaatgtcg tttgccacgt ccaacacacc ggcaacccga tgggcagcgt
     1981 gttgcgccgc tgattttttg gcgtagctgc tcacggcgcc tgacagtgtc accactcctt
     2041 cgcgcacttc gacgccaatc tgcgcccagg cgatccgaga atcccatttc aattcgcgga
     2101 gcacctgttg tttgatttcg ttatcagttt tcttacgttc cataattcct cccctcgctt
     2161 aaggcaatga tttctgcctt tgcgcttacg ggcttgtgcc ggcagcttgt gcttgtgaca
     2221 ccgcaaacgg atgagcgctg atagttttgt aagtgttaac tacgtccgcg taagcaagct
     2281 ccggcatctt cccctttttc gtttcctgcg cttttagcac gatgtcgttc atcgatagca
     2341 tgcctttcag ctctccttcg gcactcacga ccggcaagcg ccgcaccttg tgctctccca
     2401 taattttgag agcttcatgg acgtcatctt cgggagttac cgagtaaacc gcacccgaga
     2461 ttacttgctc tgcggagatg gcagcggggt tgcaatcgcg cattgcgact gccatgcaga
     2521 tatctcggtc cgtaatcaac cccacgatct ctttgccgtc tttgagcaca ggcaatacgc
     2581 cgcaatcggc ctcccacatt gttttggccg cctccgccaa actggcgttc aagtcgcaga
     2641 cttgggcctc acaagtcatg atgtctttga ctttcataaa agtccctcct cacagatttc
     2701 cctgttaagc gttaa
//
LOCUS       DASUEX010000529         3196 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_776286, whole genome shotgun sequence.
ACCESSION   DASUEX010000529 DASUEX010000000
VERSION     DASUEX010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3196
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_776286"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2092
                     /locus_tag="VFV61_08865"
     CDS             <1..2092
                     /locus_tag="VFV61_08865"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX4900630.1"
                     /translation="GDRIPIYTPRGIQEFTVRAFFRTTGASVVFGGNIALMDIHAAQA
                     AFDRVGKLDRIDIMTDPAANVGAVQQSLRERLPPGFEVAKPDRRGESLEKAVSAMHLS
                     LLIMSLLAVTVGVFIIFNSLSVSVNQRFKEIGVLRSIGVEGHRVQKMFLVEAGVLGLV
                     GSAVGVGLGFGLAGGATKIMSNVSAQVYGLASTPQPPAFRWDFAVTAFAIGVVCSLLA
                     AWLPARAASRLSPIQALHNIEVQRGEGVLGWSRFIFGLLFIVVGLTLSVFAPPGVGLM
                     VHFTFSLMVQFGLILLLPKFVVWGSRILRPLMGRLFGAEGLMAIDAAVRMPRRTSATV
                     GSLMLGLAFVFSVGAFILSHKSALDRMLDRTVNADLTIATSDQLRSRTYHFSEEQERR
                     VASLSGVKNTDGMRVTAVAYENDEIMVIARNMGLWFSNSPGLLDEGDARSAQQQTMKG
                     EGFVISQNLSFKNDLHVGDQLSVETPKGPLSRPVLGVVEFYYVEKGSVFIDRSLYKEY
                     WGDNSVDYILLNLQPGVDRTWFKGEINRAMAGEQRAFIYTSEELKAWHGRMTDQFFTL
                     TYLEMLIASFVATMGLVNMLVVSVSERQRELGILRALGGLRSQVRKIVILEAVVISVV
                     GFATGVIAGIFNGYFLVRTAARLIAGFNLRFQFPLTVVLLALPLVIMVAIAAAWWTAQ
                     RAMRLRVVEAIGYE"
     gene            2438..3160
                     /locus_tag="VFV61_08870"
     CDS             2438..3160
                     /locus_tag="VFV61_08870"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX4900631.1"
                     /translation="MTNILIFGASRGLGAAFSSGLPTRGDTVWLVSRSQPENLQRNDG
                     VERVWIKADLSSPKEASRKISEALSKQRLDVLIYNAGIWEEHAFTSDYSFEAAGIEES
                     EDIIAVNLTSAIICTQKLLSNLKQSDNAKVILIGSTSGLENSGSQEVAYAASKFGLRG
                     LGHALRENLRNFGITVTCINPGEVAAEIAYEEGVERAIEAYAGTRIPVQDLVMVVKCI
                     MGLSKVSCVKEINIPAITDLNA"
BASE COUNT          681 a          800 c          919 g          796 t
ORIGIN      
        1 cggcgaccgc attcctattt acacaccacg tggcatccag gagttcactg tgcgcgcttt
       61 ctttagaact accggagcaa gcgttgtatt cggcggcaac atcgccttga tggacattca
      121 tgcggcgcaa gcggcctttg atcgggttgg caagttagac cgcatagaca tcatgaccga
      181 cccagccgcg aatgtaggag cggtccagca gagcttgcgt gaacgccttc ccccaggctt
      241 tgaagttgcc aagccggatc gtcggggtga aagtctcgag aaggcggtct cggcgatgca
      301 tttgagtctg ctaatcatga gcctgctggc cgtgacggtc ggcgtcttta tcattttcaa
      361 ctcgctaagc gtaagcgtga atcaacggtt caaggaaatc ggagttctgc gatccattgg
      421 agtcgaagga catcgggtgc aaaagatgtt tctggtggag gcgggagtgc tgggcctggt
      481 tggttctgca gtgggtgtcg gtctcggctt cggccttgcc ggtggcgcta caaaaataat
      541 gagcaacgtc tcggctcaag tatacggtct ggcttccacg ccccaacctc ctgcgtttcg
      601 ttgggacttt gcggttacgg ctttcgcgat tggcgttgtc tgctctctgt tagctgcctg
      661 gcttccggca cgcgcggctt ctcgcctcag tcccattcag gcgctccata acatcgaggt
      721 tcaaaggggt gaaggagtgc tgggctggtc tcgcttcatc ttcggactat tgttcatcgt
      781 cgtcggcctg accctctcgg tgtttgcacc gccgggtgtc ggcttgatgg tccacttcac
      841 tttctcgctc atggttcagt ttggcttgat tctgttgttg ccaaagtttg tggtctgggg
      901 atcgcgcatc ctgcgaccac tgatgggccg gctctttggg gccgaggggc tgatggcaat
      961 tgacgcggcc gtaagaatgc cgcgacgtac gtcggcaacc gtgggctcgt tgatgctcgg
     1021 gcttgccttt gtcttctcgg tcggcgcttt cattcttagt cacaaaagcg ccctcgatcg
     1081 catgctggat cgaaccgtta atgcggatct gacgattgct acctcagacc aactgcgatc
     1141 gcgcacttat cactttagcg aagaacagga gcgccgcgtc gcctcgctgt caggcgtcaa
     1201 aaacaccgac ggaatgcgtg tcacggccgt cgcctacgaa aacgatgaga tcatggtgat
     1261 cgcccgcaac atgggcttat ggttctcgaa ctcgccgggt ctgctcgacg aaggtgacgc
     1321 tcgcagcgcc caacaacaga cgatgaaggg cgagggcttt gtaatctcgc agaatctatc
     1381 cttcaaaaat gatttgcacg ttggtgatca gcttagtgtg gaaactccca aagggcctct
     1441 ttcgcggccg gtgctcggcg tcgtagagtt ttactatgtg gaaaaaggtt ccgtatttat
     1501 agatcgaagt ctttataagg agtattgggg agataactcg gtagactaca tcctgctgaa
     1561 tcttcagccg ggcgtcgatc ggacctggtt caaaggagaa ataaaccggg cgatggcggg
     1621 tgagcagcgg gccttcatct acacgagtga agaattgaaa gcctggcacg ggcgtatgac
     1681 cgatcagttt tttacgctca cttatctaga gatgttgatc gcaagcttcg tggcgactat
     1741 ggggttggtc aacatgctgg tggtttcggt atcggagcga caacgcgaac tgggtatcct
     1801 gcgcgcactt ggcggcctgc gcagccaggt acgtaagatt gtcattctcg aggcagtagt
     1861 catttcggtc gtggggttcg cgactggtgt gatcgctgga atctttaacg gttacttcct
     1921 ggttcggact gccgcccggc tcatcgcagg cttcaacctt cgtttccaat ttccgttgac
     1981 ggttgtgctc ttggcgttgc cgctcgtaat catggtggcc atcgcggctg cctggtggac
     2041 tgcccagcgg gcaatgcgct tgcgcgtggt tgaggcgatt ggttacgagt agtctcaaac
     2101 ttcgcggttc cagtaaaaag tcagttaaat caatacatca agcctcgcta tgagtctttg
     2161 actcgtcacg gtcgactgcg tggttacctt cgacgacgtc agttgccgcg aaaaattccc
     2221 gttgctccgg cgactaagtg acgatctcac gctgccgaac aactcgtggg acggagcgga
     2281 aaaagcaacg ctgaacgcgg accgattaat ggtgaatcca gcgttccacg ttcagcgttc
     2341 atcattcata cttgttgttc agcgccacct ctggatcagg ctagattgat tttccgcgcc
     2401 cctgaacgac cgaggtttgt gagtattaag ctggcgaatg acgaacattc ttatctttgg
     2461 agcgagtaga ggactgggag cggccttcag ttcgggctta ccaactcgcg gcgacactgt
     2521 ttggctggtg tcgcgtagtc agcctgaaaa tctgcaaaga aatgatggtg tagaaagagt
     2581 ctggattaaa gccgatcttt cttcccccaa agaggcaagc cggaaaatat ccgaagcact
     2641 ctcgaagcaa aggctggatg tcttgatcta taacgccggg atctgggagg agcatgcctt
     2701 cacgagcgac tatagttttg aagcagcagg cattgaagaa tcagaggaca ttattgctgt
     2761 aaatctgacc tcagccatca tctgtactca gaaattgctg tcaaatttga agcaatcaga
     2821 taacgccaag gtcattctaa tcggctcaac ttccggtctc gagaatagcg gctcgcaaga
     2881 ggttgcgtat gctgcgtcta agttcggtct gcgtggttta ggacatgcct tgcgtgagaa
     2941 tcttcggaac ttcggcatta cggtaacctg tattaatccc ggcgaggttg ctgccgaaat
     3001 agcgtacgag gaaggtgttg agagagcgat tgaggcctac gcgggcactc gcatccccgt
     3061 ccaggactta gtaatggtgg tgaaatgtat aatggggcta tctaaagtat cctgcgtcaa
     3121 agaaatcaat attccggcga ttaccgacct aaatgcgtag gcacaactca acaaccgatt
     3181 gcagcgcgac gcaggt
//
LOCUS       DASUEX010000530         2726 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_776801, whole genome shotgun sequence.
ACCESSION   DASUEX010000530 DASUEX010000000
VERSION     DASUEX010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_776801"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="VFV61_08875"
     CDS             <1..239
                     /locus_tag="VFV61_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900632.1"
                     /translation="QKTFVLDDFRRASFYYKGKQEQFTLRSQDKGQADEVKAVCAMVL
                     EGQPSPIPLAELAATTRATFRMVDSLRTGERESV"
     gene            428..2362
                     /gene="asnB"
                     /locus_tag="VFV61_08880"
     CDS             428..2362
                     /gene="asnB"
                     /locus_tag="VFV61_08880"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEX4900633.1"
                     /translation="MCGIAGIVSHDPESKVAAMLETIEHRGRDDHGVWTSALANSGQR
                     ICLGHRRLSIIDTSAAGHQPMFSADGRYAITFNGEIYNYRELREQLKFKGHKFTTQTD
                     TEVLVAAFAEWGTDCLPRLNGMFAFGIWDNQEQRLTLARDHVGIKPLYYANLPAQEGK
                     TGSLIFASEIKAILATGLITKAIDIEALHQFLTFLWAPDPNTLFQGIKTVPPAHVLTF
                     HDGQISLNEWWDVSFDDIDNGRSEAWWQEQVLETLDRVVEMEMVADVPLGSFLSGGID
                     SSGIVAMMKRHEAGRRIGTYTVGIESEDLRYDIIPDDVKWARRVNEHLDTDYHEIMLK
                     PAVAELLPKLVYHMDEPPIDMAIPSYLVSRAARETLTVMLSGMGGDEVFAGYPRQLAM
                     KLASALDPVPGLLRRPLMRTLAATLPGGMPGKLTAPLRNAKKFARSGGLDFEDRYLGY
                     GTYFTDDAKQDLYADEIREATRGLDAYATHRRYFARVEQADPLNRLLYVDLKTFLPCL
                     NLITTDKTSMAANLEVRVPFLNREMIEFAARMPVDLKLRGFKRKYILKRALEKVLPHD
                     VVWRKKAGFGAPIRSWLRGPLKPLVNDLLSEETVRKRGLFRPEEVRRIINSNLSGRED
                     YNLQVFQLLNLELWQRQFMD"
     gene            2491..>2726
                     /locus_tag="VFV61_08885"
     CDS             2491..>2726
                     /locus_tag="VFV61_08885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900634.1"
                     /translation="MRFTASSLRGRAGRAGILILTLLVFAGASALSIPGISHAARARV
                     AALVSLGNPTPVAPTSNTKKLSLTTRDLIYDPVG"
BASE COUNT          606 a          744 c          741 g          635 t
ORIGIN      
        1 agcagaagac ttttgtgctc gatgatttcc gtcgcgccag cttttactac aaaggcaaac
       61 aagagcagtt cacgcttcgc agtcaggaca agggtcaggc ggatgaagtg aaggctgtgt
      121 gtgcgatggt gcttgaaggc cagccgtcac caattccttt agccgaactg gcggccacaa
      181 cccgggctac ttttagaatg gttgattcac tgcgaacagg tgaacgcgaa tcggtttaga
      241 gcttggggct tctgaggcat gctcagtcgg gaaggcgggc ttgcccccgc tcggttgagt
      301 ccctccagtc ctttgcggcg gcaagccggc cttcctgact gaacgttact cacgagctcg
      361 tttcaaccca tcttgagacc tgaaacctga gacctgagac ttaggacctg aaacctgaaa
      421 ccaacgcatg tgcggcatcg ccggaatagt tagtcacgac ccggaatcca aagtcgcggc
      481 gatgcttgag accattgagc atcgcggacg cgatgaccat ggtgtttgga cttcagcgct
      541 ggcaaactcc ggccaaagaa tttgcctcgg ccaccgtcgt ctctcgatca tcgatacttc
      601 agccgccggt catcaaccga tgttttccgc tgacggccgc tacgcaatca ccttcaacgg
      661 cgaaatctac aactaccgcg aactgcgtga acagctaaag ttcaagggcc acaagttcac
      721 aactcaaact gatactgaag tcctcgtcgc agcttttgcc gagtggggca cggattgttt
      781 gccgcgactc aacggcatgt ttgcttttgg catctgggac aatcaggaac agaggctgac
      841 gctcgcgcgc gatcatgttg gtatcaagcc gctttattac gcaaatctac ctgctcaaga
      901 gggtaaaacg ggttcgttaa tcttcgcctc cgagattaag gcgattttgg cgacgggtct
      961 gatcaccaaa gcaatcgata ttgaagcgct gcaccagttt cttaccttcc tctgggcccc
     1021 tgatcccaac acattgtttc agggaatcaa gactgtaccg cctgcacacg ttctgacatt
     1081 tcatgatggt cagatctcgc tcaacgagtg gtgggatgtt tccttcgacg acatcgacaa
     1141 cggtcgcagc gaagcatggt ggcaggaaca ggttctggaa actctcgacc gtgttgtgga
     1201 aatggagatg gttgcggatg tgccgctcgg ctcgttcctg tcgggcggca tcgattcgtc
     1261 cggcattgtc gcgatgatga aacgtcatga agcaggccgg agaattggaa cctacacagt
     1321 ggggatcgag agtgaggatt tgcgctacga catcatccct gacgacgtga agtgggcgcg
     1381 aagagttaac gaacacctcg acactgacta tcacgagatc atgctcaagc ctgcggtcgc
     1441 tgagcttttg cccaagctcg tctatcacat ggacgagccg ccgatcgaca tggcaattcc
     1501 cagttaccta gtgtcgcgtg cggcccgcga aacgctcact gtaatgcttt ccggaatggg
     1561 tggtgatgaa gtctttgccg gttacccacg acagctcgcc atgaaacttg cgagtgctct
     1621 tgatcccgta cctggtttgt tgcggcgccc gctcatgaga acactcgcgg cgacactgcc
     1681 cggcgggatg ccgggaaaac tcacagcgcc gctgcgcaac gcgaagaagt ttgcgcgcag
     1741 tggcggtctg gattttgaag acaggtacct gggctacgga acttacttca ctgacgacgc
     1801 taaacaggat ctctacgcag atgaaatccg tgaagcgact cgtggtctcg atgcttacgc
     1861 tacacatcgg cgttactttg cgcgcgtgga gcaagcggac ccactcaatc gtctgcttta
     1921 cgtcgatctg aaaacctttc tgccgtgtct gaatctgatt acgaccgaca agacttccat
     1981 ggcggcaaat cttgaagtgc gcgtgccgtt tctcaatcgc gaaatgatcg aatttgccgc
     2041 gcgcatgccg gttgacttaa agctacgtgg ctttaaacgg aagtacattc tcaagcgcgc
     2101 gctcgagaaa gtcttacctc acgatgttgt ctggcggaag aaggcgggtt tcggtgcgcc
     2161 gattcgttcc tggttgcgag gcccgttgaa accactcgtc aacgatctct tgtccgagga
     2221 gaccgtgcgg aagcgtgggc tgttccggcc agaagaagtt cgtcgcatca tcaactcgaa
     2281 tctctctggc cgcgaagatt acaacctgca ggtgtttcaa ctgctgaacc tcgaactctg
     2341 gcagcggcag tttatggatt gaaatgctag tatggtctcg cgttgcggat ctgaagttaa
     2401 ccctctgagt cacctgcagc taaccagttc ctttcaaacc cggtgaagaa cagggcactg
     2461 aactagtctg acaaggaagt ctttgcggta atgcgtttta cagcctcctc cttacgtggt
     2521 cgtgctggtc gtgccggaat tctgattctc accctgcttg tgtttgcagg cgccagcgcc
     2581 ctttcaattc cgggaatttc ccacgcagct cgggctcgag tcgcagcgtt ggtttcactc
     2641 ggcaatccca cacccgtcgc tccgacaagc aataccaaga agttgtcact gacgaccagg
     2701 gatctgatct atgaccccgt tggtca
//
LOCUS       DASUEX010000531         5013 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_429909, whole genome shotgun sequence.
ACCESSION   DASUEX010000531 DASUEX010000000
VERSION     DASUEX010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5013
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429909"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(131..2863)
                     /gene="polA"
                     /locus_tag="VFV61_08890"
     CDS             complement(131..2863)
                     /gene="polA"
                     /locus_tag="VFV61_08890"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEX4900635.1"
                     /translation="MPTKQAAAGEKPVERVFLIDSMSHIFRAFFAPMANRVAALSNSK
                     GQVTQAVYIFTNMLRKLLADEKPEYIAAIFESKERTFRHETFAGYKANRLDMPPDLAS
                     QLPYIIRLCEALNIPIINSPGYEADDVIGALATQAAARGLKAVIVSNDKDMCQLVRDP
                     LIVGMRQNSQVVKRKEPVPPIEWCDEEWVEKKFGVPADKVVDLLGLMGDSIDNIPGAP
                     GIGSKGAVQIIQQFGSIENALEHWQDVKHKTYRESLRDNAEMIRQSRELARIRIDLDI
                     KLDLDLIKSRPPDRAAAYELFRELEFANLTREFADAATQPAAAAPSTLQYSVVRTSAE
                     LERLLKSLWDAESVGIAVSDPTPAETAEQQCLSYDLAAGMAFATATGVSAYVDLENFK
                     EGQTVALDALREFFANGLSEKSIHDLKRAVGLLSRLNISIEGVKDDTFLAAYLLDPNR
                     SKYELIDLAREALEADSFDAAPAGWQPLQWETALAADLTARTAKVLHERILEKKLETI
                     YSEVELPLAPLLYKMEQAGLKVDPAVLAELSSYLGQELHKLTLQIYGLAGREFNIGSP
                     KQVGDVLGELNILSGRKTSTGRVSTSKAVLEELAQTHELPRLVIEYRELDKLKSVYTD
                     ALPHQIAADGRIHCLLNQTVAATGRLSSSDPNLQNIPIRTELGRRIRRAFVAEPGNKI
                     ISAEYSQLELRLLAHITQDEVMLEAFQKGEDIHKRTARLVFGAKTDAELKEARRFAKI
                     VNFAIAYAIEPWGLSQRVGISRAEAKKVIADYYNTYKGVRRYMEEVPLRAREHGYVRS
                     IYGRIRPLPGISDRNANIRKAAEREAINMPIQGTASDIVKIAMLHVAEEFKRANLSAR
                     LLMQVHDELLVESPAAESERVAQILKREMESAVQLDVPLVVDVGVADNWMDAKP"
     gene            3092..4009
                     /gene="lpxC"
                     /locus_tag="VFV61_08895"
     CDS             3092..4009
                     /gene="lpxC"
                     /locus_tag="VFV61_08895"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:TIGR00325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HEX4900636.1"
                     /translation="MIKQTTLGSSVETDGVGLHTGVEVKVRLLPAPPDTGYVFRRTDL
                     GGFEIPATVESVAHCGYATTLMRTGVMLSTVEHLLAALRGANVDNVFIEVDNLEIPIL
                     DGSAEVFTDMIGRAGVVTQPLARRALLVRERISFEQGNRRISVEPADNYEIDCLIDFP
                     HPLIRVQQFSLQLTNGSFSREIAAARTFGFSEEIEALRRSNLIRGGSLENAIVLTPDG
                     MLNQTGLRFRDEFVRHKILDIIGDLALLGMPILGRVVAERSGHILHAGLMSKLLRVRS
                     AWDIVDMPTPAATLLPAEAKAADAPTFTS"
     gene            complement(4109..4837)
                     /locus_tag="VFV61_08900"
     CDS             complement(4109..4837)
                     /locus_tag="VFV61_08900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900637.1"
                     /translation="MSVVVKSCRSRFVVPLIVVLLGTCWPVFAQGNKAASKPARTGEA
                     TVTLSEQFFNSFLEAIFDNLKAPSTPLVITASDRNRTDESAQTCPSVLTLQREHDKVK
                     TAVKFEPGRMVAPLAFTGSYNSTLLGCLEFRGWAQTEWSLEFDRSAQTLQARIKITDI
                     HLENIPTLARGSVLQLVQSAIDARINPLKVLRPEQLSSVVPIAPAGGSLRLRAREVKP
                     EIVPGSVHLRITYEFLPEQEMQNK"
BASE COUNT         1098 a         1389 c         1347 g         1179 t
ORIGIN      
        1 ggcattgtgc tcgtttgcag gggcaccctg gttgggcttg gctgattctg actgggccat
       61 gctctcccga tcgtgagtcc ttaatgtatt tggggcaaaa agaactatag gggatttgcg
      121 actggcaagc ctagggctta gcgtccatcc aattatccgc cacaccgaca tcgacgacca
      181 gcggcacatc aagttggact gcagactcca tttcgcgctt gaggatttgg gccactcgct
      241 cactctccgc tgccggagac tcgaccaaaa gttcgtcatg aacctgcatc agtaatcgcg
      301 cacttaagtt ggctcgtttg aattcttcgg cgacatgaag catggcaatc ttaacgatgt
      361 cgctggccgt tccctggatc ggcatgttga tcgcctcacg ctcggccgcc tttctgatat
      421 tcgcgttgcg atcgctgatg ccgggaagcg gacgaatgcg accatagatt gaacgcacgt
      481 aaccgtgctc gcgggcgcgc aagggcacct cttccatgta acgccgcacg cctttgtaag
      541 tgttgtagta gtcagcaatc actttcttcg cttcggcgcg cgagatcccg acgcgttgcg
      601 acaaccccca cggctcgatg gcataagcga tggcaaagtt gactatcttt gcaaagcgcc
      661 gcgcctcctt cagctcggca tcggtcttgg cgccgaagac gagtcgcgcg gtgcgcttat
      721 gaatgtcctc gcccttctga aacgcttcca gcatcacctc gtcctgggtg atgtgcgcaa
      781 gcagtcgcag ctccagttgc gagtactccg cggaaatgat cttgttgccc ggttcggcga
      841 cgaacgcacg gcgaatgcgg cggcccagct ctgtgcgaat gggaatgttt tgcaggttgg
      901 gatcgctgga tgaaagccgg ccggtagccg ccactgtttg attgaggagg caatgaattc
      961 taccgtcagc cgcaatctgg tgtgggagcg catcggtgta aacgctcttc agtttatcga
     1021 gctctcgata ctcgatcacc agacgcggta gctcatgagt ctgagccagt tcctcgagca
     1081 ctgccttact cgtggaaact ctgcctgtgg aagtttttct accggaaagg atgttgagtt
     1141 cccccagcac gtccccaacc tgtttcggcg aaccaatgtt gaattcgcgt ccggccagcc
     1201 cgtagatttg caacgtcaac ttgtgcagct cctgaccgag atagctcgat agttccgcca
     1261 acacggccgg gtctactttc aaacccgctt gctccatctt gtagagcagg ggtgccaggg
     1321 gcaattccac ttccgaatag attgtttcga gtttcttttc gagaatccgc tcgtgtagaa
     1381 ctttggctgt acgagcagtg agatcggcgg ccagggcagt ttcccattgc agcggttgcc
     1441 aacccgcagg agctgcatcg aagctatcag cctcaagcgc ctcgcgcgcg agatcaatca
     1501 attcgtactt gctacggttc ggatccaaaa ggtaagcagc aagaaaggta tcgtctttaa
     1561 ccccttcgat ggagatgtta aggcgcgaca gtaatccaac cgctcgcttc aaatcatgga
     1621 tcgatttctc gctcaagccg ttggcaaaga actcccgcaa ggcatcgagc gccaccgtct
     1681 ggccttcttt aaaattctcc aaatcgacat aagctgaaac gcctgtagct gtcgcaaagg
     1741 ccattcccgc ggcaagatcg taactgagac attgctgctc ggcggtctca gccggcgtgg
     1801 gatcgctcac ggcaattccg acactttccg catcccacaa cgatttaagc agacgctcta
     1861 actcggcact cgtgcgcacg acgctgtatt gcaaggtaga aggcgccgcg gcagcaggtt
     1921 gggtggcggc atcagcaaac tctcgtgtca ggttcgcaaa ctccagttcg cgaaacagct
     1981 cgtaggcggc ggcccgatcc ggaggccggc ttttgatcaa atcgaggtcg agcttgatat
     2041 caaggtcgat cctgatacgc gcaagttcgc gcgactgccg gatcatttca gcattatcgc
     2101 gcagcgactc gcgataggtt ttgtgcttta cgtcctgcca gtgctcgagg gcgttctcga
     2161 tcgaaccaaa ctgctgaatg atctgcactg ctccctttga accgataccg ggcgcgccgg
     2221 ggatgttgtc gatcgagtcg cccatcagtc ccagcaagtc aactaccttg tccgccggta
     2281 cgccgaattt cttttcaacc cactcttcgt cacaccattc gatcggaggc actggctcct
     2341 ttcgtttgac cacctgagaa ttctgtcgca ttcccacaat caatggatca cgaacgagct
     2401 gacacatgtc tttgtcgttg gaaacaatta cggcctttag tcccctggcc gccgcttgcg
     2461 tcgccagcgc tccaatgacg tcgtcagctt cgtaacccgg tgagttgatg atgggaatgt
     2521 tgagggcctc gcagagccgg atgatgtaag gaagttgtga agcgaggtct ggcggcatgt
     2581 cgagccggtt cgctttgtag ccggcaaagg tctcgtgcct aaaagttctt tcctttgact
     2641 cgaaaatcgc agcgatgtac tcgggctttt catcagcgag caacttgcgc agcatgttgg
     2701 taaaaatgta gacggcctgg gtcacctgac ccttggagtt tgagagggcg gccacacgat
     2761 tcgccatcgg cgcaaagaat gcgcggaaga tatgcgacat ggaatcgatc agaaaaaccc
     2821 gctcaactgg tttttcacca gcggcagctt gcttggttgg catgtgtttc gatcatagag
     2881 acagaggttt gaagtttcaa gttgaccctg gatctacggc ttggtcttgg gactttgatg
     2941 ggagtgtggc tgttttctcg ttggctgttg tgcaaatgca acactaatct gcaaagcgct
     3001 tcgaaatttg cgagtttcct gctggcatag tcattgcgct gcgtttattc gaagttcaaa
     3061 gttcgaagac caatctttga aggacatccc cttgattaag caaaccactc tcggttccag
     3121 cgttgaaact gacggcgtag gactgcacac aggagttgaa gtcaaggtgc gcttgctgcc
     3181 ggcgccgcct gacacgggat acgtctttcg ccgcaccgat ctgggcggct ttgaaattcc
     3241 agcgacagtg gaatccgtag cccattgcgg ttacgccacc acgctgatgc gtaccggggt
     3301 catgctttca accgtagagc acctgctggc agcattgcgt ggagccaacg tcgataatgt
     3361 tttcatcgaa gtcgacaatc tggaaatccc gatcctcgac ggttccgcgg aagtcttcac
     3421 agatatgatt ggccgggctg gggtggtaac acagcccctg gcgcgccgcg cactgctcgt
     3481 gcgcgagcgc atttcatttg aacaggggaa tcggcgaatc agtgtcgaac cagccgacaa
     3541 ctatgaaatc gattgcttga tcgattttcc gcatccgctg attcgcgtac agcagttttc
     3601 actgcaactt acgaatggca gcttcagccg cgaaattgcc gcggcccgta cttttggttt
     3661 tagtgaggaa attgaagcgc tgcgacgatc gaatttgatc cgtggcgggt cgcttgaaaa
     3721 tgcgatcgtg ctgacgcctg atggtatgtt gaatcaaaca gggttgcgtt ttcgcgatga
     3781 gtttgtgcgg cacaagatac tcgacatcat cggcgatctg gcgctactgg gaatgcctat
     3841 tctgggacga gtggtcgccg agcgcagcgg tcacatcctg catgcgggtc taatgtcgaa
     3901 actgctgcgc gtacgttccg cctgggacat cgtagacatg ccgactccgg ctgcgacttt
     3961 gctcccggcg gaagcgaaag cagctgacgc tccaaccttt acatcctaat cgagcggttg
     4021 aaagcgaaat aagccacaga tccaacggat acacacagag cagaaactgc gactattcag
     4081 ctccgtgcaa tccgcgagaa gctgtggctt atttgttttg catctcctgc tcggggagaa
     4141 attcgtacgt tatccgcaga tggaccgagc cgggcacgat ctcaggcttt acttcccttg
     4201 cccgcaatcg cagagaccca ccagcgggcg cgatgggcac gacactcgac aactgctccg
     4261 gccgtaacac ctttaacgga ttaatccgcg catcaatcgc ggactgcacc agttgcagca
     4321 ccgatccgcg cgccagcgtc ggaatattct ccaaatggat gtccgtaatc tttatccgcg
     4381 cctgtaatgt ctgcgcactg cgatcaaact ccaggctcca ctcggtctgc gcccagccgc
     4441 gaaactccag gcaaccaagc aacgtcgaat tgtaagaacc ggtaaaagcg agcggtgcca
     4501 ccattcgtcc cggttcaaac ttcaccgccg ttttcacctt gtcgtgttcg cgttgcaaag
     4561 tgagcacgct cggacaggtc tgcgctgatt catccgttcg attcctgtcg gatgcagtga
     4621 tgacaagcgg cgttgaaggc gccttgagat tgtcgaaaat cgcctccaga aaggaattga
     4681 aaaactgttc gctcagcgtc acagtggctt caccagtacg cgcgggtttg cttgccgctt
     4741 tatttccctg cgcgaaaacc ggccaacaag ttccaagcag gaccactatc aagggaacaa
     4801 caaatctgga acggcaagat ttaacaacaa cgctcatgtt catttctctc ttttgacacc
     4861 cataccgtct gaattgagtt gagactttgg acctgggact tgagacttgg cctcgttaga
     4921 gcccacttcg aacaatgtcg tgcaagcgaa ttagtccaac acaaactttc tcgccatcaa
     4981 caacgggaag cacggaaatt tgtgacggcc gat
//
LOCUS       DASUEX010000532         3353 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1682651, whole genome shotgun sequence.
ACCESSION   DASUEX010000532 DASUEX010000000
VERSION     DASUEX010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3353
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1682651"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /locus_tag="VFV61_08905"
     CDS             complement(<1..541)
                     /locus_tag="VFV61_08905"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX4900638.1"
                     /translation="MKKTNFVKLKLREASFISRALLSSRHPVLAHLIPMRRCNLACGY
                     CNEYDHNSSPVPPDVIKQRVDKLASLGTSIITISGGEPMMHPQLDDIIHHIRLKGMIA
                     GLISNGYYFTPERIKRLNRAGLEYLQISIDNVDPDEVSRKSLRVLDKKLRYLSEYADF
                     HVNINSVIGGGIKNPQDALT"
     gene            complement(703..1404)
                     /locus_tag="VFV61_08910"
     CDS             complement(703..1404)
                     /locus_tag="VFV61_08910"
                     /inference="COORDINATES: protein motif:HMM:NF015510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HEX4900639.1"
                     /translation="MKKLVPLSVLAIVLGGLLVVSPASANAQSAGLVSSVLNKMEKNR
                     QSLKSLRAGLSMAKYNAQLRDEEKYEGIVAYLPGSGREASVRIEWRSPQHEILAVSNG
                     KYTLFRPRLKMAYVGNSKDGKVRRNTGNIMNMLYMSRRQLEAEFQPVKDVREETLWGG
                     VSTIHLTLVPKGNASFKYAEIWVDSAGMPVQTKIVEKNDDATTMRLSDLRKNVPISSG
                     EFSLKLESDVKIIKS"
     gene            complement(1465..1794)
                     /locus_tag="VFV61_08915"
     CDS             complement(1465..1794)
                     /locus_tag="VFV61_08915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900640.1"
                     /translation="MASFLKKIFFWNYARNTWQWDMLCVLILVFIFLTPKGWFSTSER
                     VRAEEHPKQVSTLIVDAEVIDNKADRAEIQRRIRALTGRPEIEVLNVRKSFDSEGKPR
                     GYEVDIR"
     gene            complement(1889..2581)
                     /locus_tag="VFV61_08920"
     CDS             complement(1889..2581)
                     /locus_tag="VFV61_08920"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4900641.1"
                     /translation="MRTILNAENLVKTYRVGKVDVPALRGVSLQVQEGEFVAIMGPSG
                     CGKSTMLHLLGGLLTPTSGRILIDGEDLTAASDAKRTDIRRRKIGFVFQRFNLFPTLT
                     AEGNLRLAERIHGNGGEDPVRRREVLRLLGLEDKLHHKPLELSGGEQQRVALARAVVT
                     RPAIVLADEPTGNLDTENSALVLNMFQELNYRFNQTIIMITHNPEAAAMCRRIVQMRD
                     GHIVQPAATVQM"
     gene            complement(2688..3242)
                     /locus_tag="VFV61_08925"
     CDS             complement(2688..3242)
                     /locus_tag="VFV61_08925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900642.1"
                     /translation="MSEKETVSALSNLRPQHLTDALMINPIELSANYIYAQSEFFLEE
                     SDTRPQAKKGSRVVRAYYVLEELAQPKDGESQLRRRFWFDRVGGIRLAKLQTFNERGL
                     LTTEVAYWDEKPFGENAQLRLPSRIEITRPHDQYKLSITYQAPSSVEINRAYRPEAFV
                     LENRWQLPEVDLDTRQDEKMTSNQ"
BASE COUNT          785 a          917 c          808 g          843 t
ORIGIN      
        1 ctgtcagcgc gtcttgtgga ttcttgattc caccaccaat gacggaattg atgttcacgt
       61 ggaaatcggc gtattcagag agatagcgca gtttcttgtc gagtacgcgc agactcttgc
      121 gcgacacctc gtccgggtca acattgtcga tgctgatctg gagatattcc aatccggccc
      181 ggttaagccg cttgatgcgt tcaggagtga agtaatagcc atttgagatc agacccgcaa
      241 tcattccctt gagacgaata tgatgaatga tatcgtcgag ttgcgggtgc atcatgggct
      301 caccaccgga aatggtgatg attgaggtgc cgagcgaagc cagtttatcg accctctgtt
      361 tgatcacatc cggtgggact ggtgacgaat tgtgatcgta ttcgttgcaa tagccacagg
      421 ccagattgca ccgtcgcatc ggtatcaggt gcgccagcac agggtggcgg ctcgagagca
      481 gggctcggct gataaagctt gcctcgcgca acttcaattt cacaaagttg gtttttttca
      541 ttccgacgat ttaatggacc cgtaccggca aataggttcg cagaatatgc gactaaacac
      601 ttcgttggca agcagtgacg attcactcaa gcgagccaaa aagagaaagg ggctctggtc
      661 caagctgaac agagccccgg cttccgacct cgttagagat cgttagctct tgataatctt
      721 tacatctgac tccagcttga gactgaattc gccggatgag atcggcacgt tctttctcag
      781 gtcactgaga cgcatagttg ttgcatcgtc attcttttcg acgatctttg tttggaccgg
      841 catgccagcg gaatcgaccc agatctcagc gtatttgaag ctcgcgttac ccttaggcac
      901 caacgtcagg tgaatcgtac taacaccacc ccacaaggtt tcctcacgaa catccttcac
      961 cggctggaat tctgcttcca gctgccggcg cgacatgtaa agcatgttca tgatgttgcc
     1021 agtgtttcga cgcaccttgc cgtctttcga attgccgaca taggccatct tcaaccgggg
     1081 ccggaacaac gtgtatttac cgttggaaac cgccaaaatc tcatgctgtg ggctgcgcca
     1141 ttcgattctc actgacgcct cacgtccact tcccggaaga taagctacga tgccttcgta
     1201 cttctcttcg tcccgaagct gggcgttgta tttggccatg ctcaagcctg cccgaaggga
     1261 cttcaagctc tgacggttct tctccatctt attgagaacg gaactgacta aaccagcgct
     1321 ctgggcgttt gcgctcgccg gactgactac caacaaacca cctaaaacta tcgccagaac
     1381 actcagggga accaatttct tcatatttac tcctcaaacc caaaacccgg gggcaagcaa
     1441 aaacctcgca attataaggg atgatcagcg tatgtcaacc tcgtacccgc ggggcttacc
     1501 ctcactatca aaggactttc ttacgttcaa gacctcaatt tcaggacggc cggtcagcgc
     1561 tcttattcgc cgctgtattt cggctctatc cgctttatta tcaataactt cagcatcgac
     1621 gataagcgtc gacacttgtt ttggatgctc ctcagcacgc acccgttcgc tcgtggaaaa
     1681 ccaacctttt ggcgtcaaga aaataaacac caggatcagg acacagagca tgtcccactg
     1741 ccaggtattt ctggcgtaat tccaaaagaa aatctttttg agaaagctgg ccaatttcta
     1801 atatccccgg aaatcaacag caaatcccgc acctctatta taggcgcaaa cctacttcta
     1861 acgctcctaa aaactgctgg aaatacgcct acatctggac agtagccgct ggttggacaa
     1921 tgtgaccgtc gcgcatttgc actattcgtc ggcacattgc ggcagcttcc gggttatggg
     1981 taatcatgat gatggtctgg ttaaagcggt aattgagttc ctgaaacatg ttgaggacga
     2041 gagcggagtt ttccgtatcg aggttcccgg tcggctcatc ggcgaggaca atcgccgggc
     2101 gggtgaccac ggcccgagcc agggccaccc gctgctgctc tcctcctgac aactcaagcg
     2161 gcttgtgatg aagcttgtct tccaatccca gcaatcgcag aacttcccgg cgcctcactg
     2221 gatcttctcc gccgttgcca tgaattcttt ccgcgagacg aagattgcct tccgcggtta
     2281 gggtgggaaa tagattgaat cgctgaaaca caaagccgat cttacgccgc cgaatatcag
     2341 ttctcttggc gtccgaggcc gctgtcaggt cctcaccatc gatcaggatt cttccgcttg
     2401 tgggtgtaag caggcctccg agcagatgca gcatggtcga ctttccgcac ccggaaggtc
     2461 ccatgattgc cacgaattcg ccctcctgca cctgcagcga aaccccacgc aaggcaggga
     2521 cgtcaacctt gccaacacgg taagtcttta ccaaattctc cgcgttaaga attgtgcgca
     2581 tcgaactagt gattaatcag aaggtcccgg acatgtgaca gcaacgccag ctagcgaatg
     2641 tctgatgtta tacaatccga cacgcaatat ctagcagcaa ctaagtctca ctggttgctt
     2701 gtcatcttct catcttggcg ggtatcaaga tcaacctcgg gtagctgcca acgattctcc
     2761 aaaacaaagg cctcagggcg gtaggctcgg ttgatctcga ctgaagaagg cgcttggtac
     2821 gtgatactta atttatattg atcatgaggc cgagtaattt cgatccgtga tggcagacgg
     2881 agttgcgcgt tttcaccaaa gggtttttcg tcccaatagg caacttcggt cgtcaagagc
     2941 cctcgttcat tgaaggtctg cagcttcgcc agcctgatac cgccgacccg gtcaaaccag
     3001 aaacgcctgc gcagttgaga ttcaccatcc ttgggctgcg cgagttcttc cagcacataa
     3061 taagcgcgaa ccacacggct tccctttttc gcctgcggcc gtgtgtcaga ctcctccaaa
     3121 aagaactcgc tttgcgcata gatataatta gcgctcagct caatcggatt gatcatcaag
     3181 gcatcggtta ggtgctgtgg ccttagatta gaaagagcgc tgacggtctc tttctcgctc
     3241 attgtcttct tattagtttt gtctgctttc tgcgcggctc cgttcgtttt cagcttctcg
     3301 taaactgcac tgttggtgcc tttcaaaaag cgccgatact tgtcatcgcc ttg
//
LOCUS       DASUEX010000533         4042 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1682774, whole genome shotgun sequence.
ACCESSION   DASUEX010000533 DASUEX010000000
VERSION     DASUEX010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4042
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1682774"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(104..361)
                     /locus_tag="VFV61_08930"
     CDS             complement(104..361)
                     /locus_tag="VFV61_08930"
                     /inference="COORDINATES: protein motif:HMM:NF021873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HEX4900643.1"
                     /translation="MRSDAGGKAILEAEVTQIDRQGIWLLIRDRESFLPFENFPAFRD
                     ASVGAIHNVELLPNNQLYWRDLNIKISLESIPQGKQIPSLP"
     gene            704..1309
                     /gene="plsY"
                     /locus_tag="VFV61_08935"
     CDS             704..1309
                     /gene="plsY"
                     /locus_tag="VFV61_08935"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEX4900644.1"
                     /translation="MRIAIVLITAYLLGSIPFGYLIVRARAGADIRETGSGGTGATNV
                     SRRAGKGAGVLTLILDALKGVAAILIGRLILGETFGAITGAAILVIMGHMFPIWLGFR
                     GGKGVATGVGVFLVLAPLALVGAGVIFLAIVILTRYVSLGSITAAATIPLFVWLQHGL
                     IRPVTNIGATMCAAVITASLIIFAHRSNIQRLAQGTESKFS"
     gene            1313..2314
                     /locus_tag="VFV61_08940"
     CDS             1313..2314
                     /locus_tag="VFV61_08940"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEX4900645.1"
                     /translation="MKRIAVIGAGSWGTALGIVAARSGHEVKFWSRNPAVVNSINLDR
                     VNSLYLPGVAVPDGVIASQDIADALSESELIVIATPSHATRAVLESMSGALPGNAILV
                     SATKGIEIETGKRISQLVSEILGDLLTDRFVCLSGPSFAKEVVAQHPTAIVAASFNIK
                     AASTVQSDLSTEHLRIYTNDDVIGTELGGSVKNVMAIAAGMVAGLGFGANSVAALITR
                     GLAEMSRLALAEGGKLETMMGLAGLGDLVLTCTGSLSRNRFVGQELGKGRALTEITAG
                     MREVAEGVKTTRALRLLAQRVGVEMPIAAEVYAVLYESKTVRAAATALMSRPLREEF"
     gene            2333..2800
                     /gene="smpB"
                     /locus_tag="VFV61_08945"
     CDS             2333..2800
                     /gene="smpB"
                     /locus_tag="VFV61_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003564285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEX4900646.1"
                     /translation="MATSKEEKVLAANRAAFHEYHISDKYEAGIALTGTEVKSVMGGR
                     VQLKESYVAVRDGEAWLFNAHISPYSHGNRENHEPLRTRKLLLHKREIRRLDDAAAKD
                     GMTLVATRAYLKNGRIKIEVGVARGKKLYDKRETEMRRTVERETRAQLKERNR"
     gene            2830..>4042
                     /locus_tag="VFV61_08950"
     CDS             2830..>4042
                     /locus_tag="VFV61_08950"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF013079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEX4900647.1"
                     /translation="MQVEARSGRYQDVDVQAIAVAVFKDEKADAGFLKELDAAAGGMV
                     KAVIESEEFKGKEGETVYLHLPADAKLKTRRLLLIGAGEKQEHEAAQVSMVAGTAVRL
                     LRAKNVKSIGYVSRGDLDAETSAAAAVEGAIMALFDLDKYRTVDKEVKTVDLFVVISE
                     AEEKELTRGVRRGQVIGESVNFTRDLANEPGGNLTPTDMAERASAMAKEFGLSVDVLD
                     EARMQKEGMGSLLSVSRGSEEPAQLIVVKYTPAKSSSKDDHELLAFVGKGITFDSGGI
                     SLKPGENMELMKYDMTGGATVLGAMRAIAQLKPSVPVLGVVPCSENLPSGKATKPGDV
                     VTAMTGKTIEVINTDAEGRLILADAIAYAKKLGATKVIDMATLTGAVSIALGDVNTAV
                     LGTDQTLIDEII"
BASE COUNT          976 a          941 c         1208 g          917 t
ORIGIN      
        1 gggcttcgca agcggcgttg gatttgttac gaagaaggct gatctagatt ctcaatccag
       61 ctcaacagcg ggccgttggt aatgagatgg gtgggtaact cttttaggga agggatggaa
      121 tttgcttacc ctgtggaatt gattcaagcg agatcttaat attcagatcc cgccagtaca
      181 actggttatt tggcagaagc tcaacgttgt gaatcgctcc cacggaagca tcccggaatg
      241 caggaaagtt ttcgaaagga agaaaagatt cgcggtcccg aatcaataac cagattccct
      301 gtctatcgat ttgagtcacc tcagcttcca aaatggcttt gccacctgcg tctgatctca
      361 tcttcatttt cctcaattat gttttgggct tttcttatct cgcttaatct caggccatca
      421 ttctctgcca cttcaatcac cggttcaagc cagaacttag cctgtccatt cgcacaatag
      481 acatggatgt gcattcgtgg ttcctcattt gagaagaagg agaatcgaaa gccttgtttg
      541 cgaaagacag taggactcac gggatgaggc gtgctcctga tttgaacaaa ttgcagtatg
      601 acctgctgcc gtccaattgg gcagagattg tcgcttaaca cgacggttgc tatagtgcca
      661 gtatcctttc atgtcggaac tcattagctg agtgcagcaa cctttgcgga ttgcaatcgt
      721 tctcatcacc gcctacctgc tcggctccat accgttcggt tatctgatcg tgcgagcgcg
      781 agccggtgca gatattcgtg aaaccggatc ggggggcaca ggcgcaacga acgtttcgcg
      841 ccgggcggga aaaggcgctg gcgttttgac ccttattctc gacgccttga aaggggtggc
      901 ggcgatcttg attggtagac tgattctcgg tgaaacattc ggggcgatta ctggggctgc
      961 aattctcgta atcatgggac acatgttccc gatctggctg gggtttcgtg gcggcaaagg
     1021 agtggcgacg ggggttgggg tttttctcgt actcgcacct ttggcgttag tgggagccgg
     1081 agtgatcttc ctggcaatcg tgattctgac tcgctacgta tcactcggtt cgatcacggc
     1141 cgcagcgact attcccttat ttgtctggct gcaacatggg ttgatacgtc cggtgaccaa
     1201 cataggggcg actatgtgcg ccgccgtcat cacagcgtca ctgataatct ttgctcatcg
     1261 cagcaacatt cagcgtttgg cccaaggcac ggaaagcaag tttagttgaa ccatgaaacg
     1321 gattgcggtc atcggtgcgg gcagttgggg gacggcgctg ggcatcgttg ctgctcgatc
     1381 cggtcatgag gtgaagtttt ggtcacgtaa cccagcggtc gtaaattcaa ttaatctcga
     1441 tcgcgtcaat tcgctgtatt tgccgggcgt tgctgttcct gatggtgtga ttgcaagcca
     1501 ggatatcgct gatgctctca gcgaatcgga gcttatcgtg atagcgacgc catcccatgc
     1561 gactcgagct gttctggaat cgatgtcggg tgcattaccc ggaaatgcga tcctggtcag
     1621 tgccactaaa ggaatcgaga ttgaaacagg caaacgcatt tcgcagttgg tgagtgagat
     1681 cctcggtgat ttgctcacgg accgcttcgt ttgtttgtcc gggccatctt tcgccaaaga
     1741 agttgtggcg caacacccaa ccgccatagt tgcagccagc ttcaacataa aagcagcttc
     1801 aacggtacaa tcagacttga gtacggagca tttgcgcatc tacaccaatg acgacgtcat
     1861 aggtacggag ttaggcgggt cagtgaagaa tgtgatggca attgctgcgg gaatggtggc
     1921 cggcctggga ttcggtgcga acagtgttgc agccctaatc acgcgcggct tggcagaaat
     1981 gtcccgcctg gctttagccg aaggcggcaa gttggagaca atgatgggtc tggccggact
     2041 cggtgacctg gttctaactt gcactgggag tctttcgcgc aaccgattcg ttggccaaga
     2101 acttggtaag gggcgtgcgc tcacagagat taccgcggga atgcgggaag tcgccgaagg
     2161 agtgaaaacg acgcgggcat tgagactgtt ggcgcagcgc gtcggtgtgg aaatgccgat
     2221 cgccgccgag gtgtatgccg tcctctatga aagcaaaacg gtgcgcgccg cggccacggc
     2281 actgatgtca cgaccactgc gtgaggagtt ctaagaaagt caggtagaaa aattggcaac
     2341 ttccaaggaa gaaaaagttc tggcagcgaa tcgcgcggcc tttcacgagt atcacatttc
     2401 ggataaatac gaagctggga tagcgttaac gggaacggaa gttaagagcg tgatgggcgg
     2461 acgggtgcaa ctgaaagaat cttacgttgc agtccgagat ggtgaggcgt ggttgtttaa
     2521 tgcgcacatc tccccttatt ctcacggcaa ccgggaaaac cacgaaccgc tgcgaacccg
     2581 caaactgctg ctgcacaaac gtgaaatcag gcgtttagat gacgcggcag ccaaggatgg
     2641 catgaccctc gtagcgacgc gagcttattt gaagaatggc agaatcaaaa tcgaagtggg
     2701 agttgcgcgc ggcaagaagc tttacgacaa acgtgagact gaaatgcggc gcacggtaga
     2761 gagagaaaca cgggcccagt tgaaggaacg gaatcgttaa ggaaaattgg ttgaggagca
     2821 aacttactaa tgcaagttga ggcgcgaagt ggacgttatc aggatgttga tgtgcaggct
     2881 atcgcagtcg ctgtttttaa ggacgagaag gccgatgcag gttttctaaa ggaattggac
     2941 gctgctgctg gaggaatggt caaagcggta atcgaatcag aagagttcaa ggggaaagaa
     3001 ggcgagacgg tttatctgca cttgcctgcg gatgccaaac tcaaaacccg acggctgctg
     3061 ctgatcggcg cgggtgaaaa gcaagagcac gaggcggcgc aagtctcgat ggttgccggg
     3121 accgcagttc gtctgttacg agcaaaaaat gtgaaatcga tcggatatgt atcgcggggg
     3181 gacctggacg cggaaaccag cgccgcagcg gcggtcgaag gcgccatcat ggccttattt
     3241 gatttggata agtatcgaac agtcgataag gaagtaaaaa cggtcgatct gtttgtcgtg
     3301 atctccgagg cggaagaaaa agaacttact cgaggcgtta gacgcggtca ggtgatcggc
     3361 gagtcagtaa actttacgcg tgatctggcg aacgaacccg ggggcaattt gacgccgacc
     3421 gacatggccg agcgggcgag cgccatggca aaggaatttg gcctgagcgt tgacgtgctt
     3481 gacgaggcgc gcatgcaaaa ggaaggcatg ggttccctct tgagcgtgtc tcgtggttcg
     3541 gaagagccgg cacagttgat cgtggtgaaa tacaccccgg caaaaagcag cagcaaagat
     3601 gaccacgaat tactggcttt cgtcgggaag ggaattacct tcgattctgg cggcatttcg
     3661 ctgaagccgg gcgagaacat ggagttaatg aagtatgaca tgaccggggg cgccacggtg
     3721 ttgggcgcga tgcgagcgat cgcccagttg aagccctcgg tgccggtgct cggcgtagtt
     3781 ccttgttctg agaatttgcc gtcaggcaaa gcgacaaagc ccggcgatgt cgtcaccgcg
     3841 atgactggaa aaacgattga agtaatcaat actgacgccg aagggcgttt gatcctcgcc
     3901 gacgctattg cctacgcaaa aaaactcggc gccaccaaag taatcgatat ggcaaccctg
     3961 accggggcag tttcgattgc actgggcgac gtaaataccg ccgtgctggg gacggatcaa
     4021 actttgatcg acgagattat tg
//
LOCUS       DASUEX010000534         2950 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_527746, whole genome shotgun sequence.
ACCESSION   DASUEX010000534 DASUEX010000000
VERSION     DASUEX010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2950
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527746"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="VFV61_08955"
     CDS             complement(<1..401)
                     /locus_tag="VFV61_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HEX4900648.1"
                     /translation="MFCPRCAAQNSDDTKFCRACGTNLATVSLVLSDPQKLSAATLER
                     VASISSAKRRDGISRLIHGTGWIGASTVVGVALGLFSHTNDWIFVWLGLASWMACLGI
                     IQWSQGINKLIEARYLSRELESTMPNSSQAS"
     gene            complement(502..1020)
                     /locus_tag="VFV61_08960"
     CDS             complement(502..1020)
                     /locus_tag="VFV61_08960"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX4900649.1"
                     /translation="MNARQLFSDLFHHMEWADATVWNSVLAAPAASTDERIKNYLHHI
                     HMVQHAFINVWRAQPHSANAGSDLVLTELPEWGRQFHQLAGAYLQTLSEEDLDQPFVM
                     PWAKYLTKHLGRDPEVTTKGETILQVAAHSTYHRGQVNARLRELGSEPPLVDYIAWIW
                     FGRPAADWATAK"
     gene            complement(1055..1477)
                     /locus_tag="VFV61_08965"
     CDS             complement(1055..1477)
                     /locus_tag="VFV61_08965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900650.1"
                     /translation="MSHFLTFVFISPDEANIEQRVNELVDPYFDNEDREESPDDQIKC
                     DGFVIGGRYDQEIFGVEPLYNLTPPEFEKRYGLDVIRVEDNVRPARDVPKKLVPYAIV
                     TPQGEWFECNDFADWPAQVEQLLLEHSEYLVVAVDCHC"
     gene            complement(1509..1934)
                     /locus_tag="VFV61_08970"
     CDS             complement(1509..1934)
                     /locus_tag="VFV61_08970"
                     /EC_number="1.11.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX4900651.1"
                     /translation="MKRNAAAEWKGGLKDGKGTISTDSGVLAESQYSFSTRFEDGNGT
                     NPEELIAAAHAGCFSMALSGQLGQAGLTAESIRTTASVKLEKTDSSFAITSVHLEVKA
                     RVPGADQQAFATAANNAKAGCPVSKVLKAEITMNASLET"
     gene            complement(2100..2408)
                     /locus_tag="VFV61_08975"
     CDS             complement(2100..2408)
                     /locus_tag="VFV61_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900652.1"
                     /translation="MKTENLPIACSLTESELQQRRKEVLQQTKAAMIGVKETETGFVY
                     QFSSSSERITALADLIALEHQCCPFLSFRLTVVPGEEPVALELSGPPGTKEFLVALFE
                     "
     gene            complement(2476..>2950)
                     /locus_tag="VFV61_08980"
     CDS             complement(2476..>2950)
                     /locus_tag="VFV61_08980"
                     /inference="COORDINATES: protein motif:HMM:NF012995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HEX4900653.1"
                     /translation="HMTLLVTAPDAPQIRWLADVGFGDSFNEPLLLDSRESQQEGLRA
                     YRIDAEKDHLQFLQREGEGEWKPQYRFTLEPHEYSDYLPMCYYHQTSPQSHFTKARIC
                     TRATPDGRITLSELRFITTANGERHERSLDDEDAYRLVLREQFGIEINRWRPSPV"
BASE COUNT          663 a          823 c          753 g          711 t
ORIGIN      
        1 tcgctagcct gactcgaatt cggcattgtc gattccagtt cgcggctcag gtaacgcgct
       61 tcgatcaact tgttgattcc ctgagaccat tgaattattc ccaagcaagc catccagctc
      121 gccaatccca accagacaaa gatccagtcg ttggtgtggc tgaagagacc gagcgcgact
      181 cccaccaccg ttgaagctcc aatccagcct gtgccgtgga tcagcctcga aattccatcg
      241 cgacgcttgg ctgatgaaat acttgcgact ctctccagcg tcgccgcgga gagtttctgc
      301 ggatcagaga gcacaaggct aacggtggcc agattcgtgc cgcaggcgcg gcaaaatttt
      361 gtgtcatcac tattctgagc agcacaacgt ggacagaaca tcgcagtctc tccaagtgga
      421 tgtacgagcc cacccgaatt tcaggtttca gaaaacatta attgacgggc gtcatctgcc
      481 ccggcgaatt aacagtctac gttactttgc tgtggcccaa tccgcggcgg gtctcccgaa
      541 ccagatccag gcaatgtaat caaccaaggg cggttccgag cccagttcgc gtaaacgggc
      601 gttgacttgg ccgcggtgat aggtgctgtg cgcagcgacc tggaggatgg tttcaccctt
      661 agttgtcacc tcgggatcgc gaccaagatg tttggtgaga tattttgccc agggcattac
      721 aaaaggctga tccagatctt cctcgctgag ggtttggaga taggcgcccg cgagttgatg
      781 aaactggcgg ccccattccg gcagctcagt aagcacgagg tcgctccccg cattagcgga
      841 atgtggttgt gctcgccaaa cattaatgaa ggcgtgctgc accatgtgga tgtggtgcag
      901 gtaattcttg atgcgctcgt cggtggaggc tgccggcgcg gcaagcaccg agttccagac
      961 ggtggcgtcg gcccactcca tgtggtgaaa gagatcgctg aagagttgtc tggcattcat
     1021 tacgactcac taatcgcgat tcacgagtca cgaatcaaca atgacaatca acagccacca
     1081 ccaaatactc gctgtgttcc agcaatagct gttcaacttg ggccggccag tccgcaaaat
     1141 cattacactc gaaccactcg ccctgaggtg tcacgatcgc gtaaggaacc agcttcttag
     1201 gaacatcgcg cgccggacgc acattgtcct cgaccctaat cacatcgaga ccgtaacgtt
     1261 tttcaaactc cggtggcgtg aggttgtaga ggggctcgac gccaaaaatc tcctgatcgt
     1321 agcggccgcc gatcacgaac ccgtcgcact taatctgatc atccggtgat tcttcccgat
     1381 cctcgttgtc aaaataaggg tccaccagct cattgacgcg ttgctcaatg ttcgcttcat
     1441 cgggactaat gaagacgaac gtcaaaaagt gactcatgag tttgtacgcc tgtccgccag
     1501 atgatgagct aagtttccag gctggcattc atcgttattt cagccttgag aaccttggag
     1561 acgggacaac ccgctttggc gttgttagcg gccgtcgcaa acgcctgctg atccgcgcct
     1621 ggcactctcg ctttcacttc gagatgcact gacgtaatcg cgaaactcga atctgtcttt
     1681 tcgagtttga cgctggccgt cgtacgaatg ctttccgccg tcaagcctgc ctggccaagt
     1741 tgtcccgaca gggccataga gaagcatccg gcatgagccg ctgcaatcaa ctcttccgga
     1801 tttgttccat taccatcttc aaaccgggtg ctaaaagaat attgagactc ggcaagcacg
     1861 ccactgtcag ttgaaatcgt gcccttgcca tctttcagtc cacccttcca ttccgccgct
     1921 gcgttgcgtt tcatgaatcc tcccacccaa gttgttttcg aactttcgca ataaactacc
     1981 tcaaaaacat ccggcgtagc taaccataac gccacgatag tcgtttcctg atgttttgtg
     2041 tcgcgaaatg tcttttgcga tcgacggccc ctggcaccgc ctcggtcgcc gagctcccgt
     2101 cactcaaaca gcgcgaccaa aaactctttg gttcctggtg ggccgctgag ttcgagtgca
     2161 acaggctctt cgcccggcac gactgttaga cggaacgaaa gaaagggaca gcactgatgt
     2221 tcgagtgcaa tcaggtctgc cagagccgtg attcgctcgc tggacgacga aaactggtaa
     2281 acaaaaccgg tttcggtttc tttcacgccg atcatcgcag cttttgtctg ctgcaagact
     2341 tccttgcggc gttgctgtaa ttcggactcc gtcagactgc acgcgattgg tagattctca
     2401 gttttcatcg cagttttccc ggccttttct ttgggaatcg acgcgtgtcc tgagtgtcag
     2461 tgatttctga ttagatcaga cagggctcgg ccgccaacgg tttatctcga ttccaaactg
     2521 ttctcgcagc acaaggcgat aagcatcctc gtcgtccaga ctgcgttcat gccgttcgcc
     2581 gttagcagtg gtaataaatc gcagctcact caatgtaatg cgtccgtcgg gcgttgctcg
     2641 ggtacagatt cgagctttag taaagtgtga ctgcggcgaa gtttgatggt aatagcacat
     2701 cggaaggtag tcagaatatt cgtgaggttc aagcgtaaaa cgatactgcg gtttccattc
     2761 accttcgccc tcgcgttgca agaactgcaa atgatctttc tccgcatcga tccggtatgc
     2821 gcgtaggcct tcttgctgtg actcacgaga atcgagcagc aatggttcgt taaatgaatc
     2881 gccaaagcct acatctgcga gccaacgtat ttgcggggcg tccggcgccg tcaccaataa
     2941 cgtcatgtga
//
LOCUS       DASUEX010000535         3824 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1474661, whole genome shotgun sequence.
ACCESSION   DASUEX010000535 DASUEX010000000
VERSION     DASUEX010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3824
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1474661"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1920
                     /locus_tag="VFV61_08985"
     CDS             <1..1920
                     /locus_tag="VFV61_08985"
                     /inference="COORDINATES: protein motif:HMM:NF015655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900654.1"
                     /translation="DKHIERRANGFNVKLNRGGIREIEFIAQALQLAHGGDDDWLRVS
                     HTLISLGRLADRGLITEQERTELSDAYTFLRTLEHRLQMEHGLQTHTVPLSREQRALV
                     ARRMNFGGPDVVVELDKALELHTTNVRSAYDRVFQTTEGADEDSPYLVTRSTRSEEQL
                     LTADETACRAAAKLFAARLDPATDGEILASQLQDMALTTLNPHRALAGATRVASSLEK
                     SDSALKISPDNLAALLRLCGSSEFFGEMVASNPSLITTLTDKSTPLRRDYRSLLRSSI
                     NAEENFSAEMSALRRAWARLLVEIGEADAGGKISVSESNRLQTELGVASINVAYLVAR
                     RELVRRYGNLTGGPRISVLGLGRLASGGVDYGSDLDVVLVYDSLVQSPVASLSRDEAY
                     ARLGELMIAALSSITREGYLYRVDLRLRPDGQNGPLVISSEGFLDYLKQRTAVWEYLA
                     YVKLRAVAGDLELGKMVETHARHAIHESAARLDPEELSRETRHVRDRLEKEKTTRGRR
                     AGVDVKFSAGGMLDVYFAARYLQLRYDVPDEGDDRSTTTTLERLRATAALNTSEFEAL
                     HGGYVLLRSIDHYLRLILGRQARLPPVDHPALQDIAKLLNFKSANALHATLLEKMTGI
                     REAYDSVTGGEGSGL"
     gene            2302..2820
                     /locus_tag="VFV61_08990"
     CDS             2302..2820
                     /locus_tag="VFV61_08990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900655.1"
                     /translation="MFRIKASYTEQLELKTTIERAREFFGEFRNFVDLMPGIEGIRKE
                     AGGIMRWMVRADVPVIGPVVASFAVEKTEDEPNRLEWSPARSEVKNYLRYAAAFEERG
                     QKVLIRIAQHVELRRPHARDLHKLASLVGEGPISAEMQKRVREMIKTFLERAQAKLES
                     EPEFEEQPVPVP"
     gene            complement(3515..>3824)
                     /locus_tag="VFV61_08995"
     CDS             complement(3515..>3824)
                     /locus_tag="VFV61_08995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900656.1"
                     /translation="VAPIRFLQPRHPLLNYPNKIRSSDFNNWIQERGLYYPKEWDSHY
                     TALFASNDTGEKPLRGGLLVARYGRGRYIYTSMVWYRQLAAGVPGAYRMLANMISYGH
                     "
BASE COUNT          823 a         1047 c         1093 g          861 t
ORIGIN      
        1 gacaagcata tcgagcgacg cgccaacggg ttcaacgtca agctcaatcg cgggggcatt
       61 cgcgagattg aattcatcgc ccaagcgctg cagctggcgc atggcggcga cgacgattgg
      121 ctgcgcgtct ctcacactct aattagcctg ggccgcctgg ctgatcgcgg tttgattacg
      181 gagcaggaac gcaccgagtt gtccgacgct tacacctttt tgcggaccct cgaacatcgt
      241 ttgcagatgg agcatgggct acagactcac actgtgccgc tcagccggga gcaacgtgcc
      301 ctcgtcgccc gtcgcatgaa ttttggtggg cctgatgtgg tcgtagagtt ggacaaagca
      361 cttgagctgc acacaaccaa cgtgcggagt gcatacgacc gcgtgtttca gaccaccgaa
      421 ggggctgacg aagactcgcc gtacttggtg acaagaagca cgcgcagtga agaacaattg
      481 ctcaccgctg acgaaacggc gtgccgggcc gcggccaaat tgtttgcggc ccgactcgac
      541 cctgctactg acggcgaaat actcgcaagc caattgcagg acatggccct caccacgctt
      601 aatccccatc gcgctctcgc gggagccaca cgagtcgctt cctcgcttga gaagtcggac
      661 agtgcgctca agatttcgcc tgacaacctg gctgccttgc tccgcctctg cggttcatct
      721 gaattcttcg gcgagatggt cgccagcaac ccctcgttaa ttacgacgct cactgacaag
      781 agcacaccac tgcgccgcga ctatcgctcc ctgttgcgct ccagcatcaa tgctgaagaa
      841 aacttctctg ccgagatgtc cgctttgcgt cgtgcgtggg caagactgct ggtcgagatt
      901 ggcgaggctg atgccggcgg caagatttcg gtttcagagt ctaatcgctt acagacagaa
      961 ctcggcgtgg caagtatcaa cgttgcctat ttggttgcgc gccgcgagct ggtgcgccgc
     1021 tatggaaact taacgggtgg gccacggatt tccgtgctcg gactaggacg tctggcaagt
     1081 ggcggcgttg actacggatc cgatctggat gtggtgctgg tttacgattc actcgtgcaa
     1141 tcgcccgtgg cttctttgtc tcgggacgaa gcatacgcac gactaggcga actgatgatt
     1201 gcggcgcttt cgagcatcac gcgggaaggt tacctctacc gcgtggattt gcggctgcgg
     1261 cctgatgggc agaacggccc actcgtgatc agttccgaag gatttctgga ctatctgaag
     1321 caacgaacag cggtatggga gtacctggct tatgtgaagc tgcgggctgt tgccggggat
     1381 ctcgagctgg gtaagatggt cgaaacccac gctcgtcatg ccattcacga atccgcggcg
     1441 cgtctcgatc ctgaggagct gtctcgcgag acacgccatg ttcgcgatcg actggaaaag
     1501 gagaagacaa cccgcggccg ccgcgctgga gtcgacgtca agttttccgc cggcggaatg
     1561 ctcgatgttt acttcgccgc gcgttatttg cagttgcgtt acgatgttcc cgatgaaggc
     1621 gacgatcgct caaccacgac aacgcttgaa cggttgcgag cgaccgcagc gctgaacacg
     1681 tcagagtttg aggccttgca cggcggctac gtattgttgc gatcgatcga ccactatctg
     1741 cgcttaatcc tgggacgcca ggcccgctta ccgcccgtcg atcatcctgc cttgcaggac
     1801 atcgcgaagc tgctcaactt caaatcggcg aatgccttgc acgcgaccct gctcgagaag
     1861 atgacaggaa ttcgtgaggc ttacgattct gtgacggggg gcgaaggttc aggcttgtga
     1921 gatgggccgg gcgcattcgt gccaacaacg agtgcaccag acgtggtttc tttgtcgctc
     1981 agctaacgag tattcgcgcg cccccggcag ccgccgcaca acctcgcgct cgtcttcatt
     2041 cagctccgcc caccgctgca aacggccatg acaactcgga caaagtgatt cgtgctccat
     2101 tttgcttggt gtgacgcaaa ctgtcagttt tcgtgctgac gcagcgccgt tgcggcaaaa
     2161 caaaagtttc tggcgcggta cggcaacata atggtttacc tgtgtctcat cctcgtgtaa
     2221 tttagctttg gtgcttcgcc gagtgcaaca gagcgcctcc tgagggcatc aagataaggg
     2281 agagtggtaa cgggagaacg aatgttcagg atcaaagcct cgtacacaga acaactggaa
     2341 ctgaaaacaa cgattgagcg agcccgggag tttttcggtg agtttcgtaa ttttgttgat
     2401 ctaatgccgg gcatcgaagg tatccggaag gaagccggcg gcattatgcg ttggatggta
     2461 cgagctgacg tgccggtaat cggaccggtg gtcgcgagtt ttgcggttga aaagaccgag
     2521 gatgaaccca atcgcctgga atggtctccg gcgcgcagcg aagtaaagaa ctatttgcgc
     2581 tatgccgccg cgtttgaaga gcggggacag aaggtcctga ttcgcatcgc ccagcacgtg
     2641 gaattgcgcc ggccgcatgc gcgtgatctg cataagttgg caagtctggt gggggaagga
     2701 ccgatcagcg ctgagatgca gaaacgcgtg cgcgagatga tcaagacctt cctcgagcgg
     2761 gcgcaggcga agttggaaag cgagccggag tttgaagagc agcccgtgcc ggtgccttaa
     2821 gagttgtccc cttatgagtc cgcgaagcgg acgtctgaaa atagcccagc acttcagtgc
     2881 tgggtttttg tttaatgaag tccctagtcc gtgaaacgga cggctgattg tccttagcga
     2941 aatatgtacc tccgatcata atcgacacca tgcttgtcca acagctgttg aaactcctct
     3001 tggaatgtgc gcttgcgatg atgttccttt tgattattaa tttaagcgac tgtcttctta
     3061 acttgtgatt gacttacgct aaacgctccg tagcccctcc tgccattcaa acttcctagt
     3121 tcctagttct ccgcggaccc atttagaaga tccgcctttg attaactgta cagctttcgc
     3181 aaaactcatg gtgctgggaa gggataagag ggcgtgaagg tggtcaaaca ctcccccaat
     3241 tgccagcgcc ttcatgcggt tctcgcgtgc gatccctcca atgtagggcc acagccgtcc
     3301 ttccaactcg gcgtcgatcc agggataacg ttctttggtg ctaaaggtgc aatgcatgag
     3361 gttgcagaca tatgcgtgag gcattgtgag gctcctttca gttttcagtc gtccgcttcg
     3421 cggactatga gcttataacg gacgaaaccc agcactgaag tgctgggcta ttagcagtcg
     3481 tccgtttcac ggactcaata ttttgcatcc ctcactagtg cccgtaacta atcatattag
     3541 cgagcatgcg gtaagcgcct ggaacgcccg ccgcgagctg ccggtaccag accatactcg
     3601 tgtagatgta cctaccacga ccgtagcgcg ccaccagtag gccgccgcgc agaggcttct
     3661 cacccgtatc attgctggca aacaacgctg tgtaatgact atcccactcc ttcggataat
     3721 aaagtccacg ctcctgaatc cagttgttaa agtcgctgct gcgaatcttg ttgggataat
     3781 tcagcaatgg atgtcgcggt tgcaggaaac ggatgggcgc cact
//
LOCUS       DASUEX010000536         3095 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_430413, whole genome shotgun sequence.
ACCESSION   DASUEX010000536 DASUEX010000000
VERSION     DASUEX010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3095
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430413"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="VFV61_09000"
     CDS             complement(<1..188)
                     /locus_tag="VFV61_09000"
                     /inference="COORDINATES: protein motif:HMM:NF019241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1572 family protein"
                     /protein_id="HEX4900657.1"
                     /translation="MKESIANHYLADAISSFRAYKKLGEKAVAQLSDEEMFAIIDDES
                     NSVAIIMKHMAGNMISRWT"
     gene            complement(222..1625)
                     /gene="hisS"
                     /locus_tag="VFV61_09005"
     CDS             complement(222..1625)
                     /gene="hisS"
                     /locus_tag="VFV61_09005"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEX4900658.1"
                     /translation="MSRAKTQPARGMRDFLPADVRRREYVIGVIKEVYERYGFEPLET
                     PAVENIETLLGKYGEEGNQLIFKILKRGVHEATGEADLALRYDLTVPLARVVAEYRDR
                     LPKFFKRYQIQPVWRADRPARGRFREFYQCDVDVLGSRSPVVETELIAAASEALRTLG
                     FGDFVIRLNHRQVLIGMLLAAGVALDQHDPTLMALDKLDKIGREGVAKELSERGINDE
                     SGRRLLDFFVELQSLEHAAEITVGEDSTVKQEALNAAVLGRLVEFIGDNELGARGVDE
                     LRSIIELVEAAGVSELVRIDPSLARGLSYYTAAIMEINVKDLSGSLGGGGRYDNLVGM
                     FSGQDVPACGFSLGLERIIVVMTEREMFPEQLVASPADVMVTIADEDSVTESISLAQE
                     LRREGLRVDLYPESDKLGKQLKYASAKGIPVVIICGEDERKRHEVSIKYMHSGEQKSV
                     ARHQMIDSVRKALPSGV"
     gene            complement(1625..2368)
                     /locus_tag="VFV61_09010"
     CDS             complement(1625..2368)
                     /locus_tag="VFV61_09010"
                     /inference="COORDINATES: protein motif:HMM:NF020965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HEX4900659.1"
                     /translation="MDTYRRIFAATILVILNIAFTVCAQQPRPQLDEATTPSVSDTGV
                     EGTTEALPQTKGDEPYTLRKGHNEFGFWAGGAFQATTIFGGLHDDEARDRGLFMAAFR
                     YGRTLAANDSMALQYTLDAIPIELAIGNITSVTTVGPVTTFTRETTYGGGLTPLGLQL
                     DFMNGSRVHPFVHVNGGGLIFSKSVPLPDAGRFAFVGEAGSGVRIFTSETRAITLGVR
                     FHHISNGDRSGSNRGLNQFVVYGGFSIFK"
     gene            complement(2394..2906)
                     /locus_tag="VFV61_09015"
     CDS             complement(2394..2906)
                     /locus_tag="VFV61_09015"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEX4900660.1"
                     /translation="MIRPFRGVHPQIHPSAFVEESACVIGDVHLGEDSSVWFNSVIRG
                     DVHFIRIGNRTNIQDNSVIHVRHYSHPTIIEDQVTVGHSVTLHGCYVEKGSLIGIGSI
                     VLDDVRVGEYSIVAAGAIVSPGTIIPPHSLVMGIPARVKRTLTAEEVSGLDVFWQNYV
                     EYTRMYNEER"
BASE COUNT          726 a          907 c          754 g          708 t
ORIGIN      
        1 gtccaacgag aaatcatgtt gcccgccatg tgtttcataa tgatcgcaac gctattcgac
       61 tcgtcatcga tgattgcaaa catctcttcg tcagagagtt gcgcaactgc cttctctccg
      121 agcttcttgt aagcgcgaaa actggagatg gcatctgcga gataatgatt cgcaattgac
      181 tctttcatca ccataccccc gaaacgcaat cagcccgcgc ttcaaacgcc ggaaggcagc
      241 gccttgcgca cgctatctat catttggtgc cgcgcgacac tcttttgttc gccggagtgc
      301 atgtacttga tcgagacctc gtggcgttta cgctcgtcct cgccacaaat gatcacgact
      361 ggaatcccct tcgctgatgc gtactttagt tgtttcccca gtttgtcaga ttcagggtac
      421 agatcaacgc gtagtccctc acggcgcagt tcctgcgcaa gtgagatcga ttcagtaact
      481 gaatcttcat cagcgatggt gaccatcacg tccgccggcg aagccaccag ttgctctgga
      541 aacatctcgc gctcagtcat gactacaatg attcgctcaa gtccaagcga aaaaccacat
      601 gccggaacat cctgcccgga aaacattccc accagattgt catagcgtcc gccgccaccc
      661 agactgcctg acagatcttt gacattaatc tccatgatgg ccgccgtgta gtaagaaagg
      721 ccgcgcgcca gactgggatc gattcgcacc agctctgaga ccccggccgc ctcgaccagc
      781 tcgattatcg agcgaagttc atcaactccc cgcgcgccaa gctcgttgtc tccaatgaac
      841 tcaaccaggc gtccaagcac tgccgcgttc aatgcttctt gtttgaccgt tgaatcctcg
      901 cccacggtga tttcagcagc atgctcaagg gattgaagtt cgacaaaaaa atcgagcagt
      961 cgccttccac tctcatcatt tattccacgc tcgcttagtt ccttcgccac accttcgcgg
     1021 ccaatcttgt ctaacttgtc cagcgccatc agggttggat catgttggtc cagcgcgact
     1081 ccggcggcca gtagcatgcc aatcaagacc tgccggtggt tcagtcgaat cacgaaatcc
     1141 ccaaatccca aggttctcaa ggcctcgctc gccgctgcaa tcagttccgt ttcaaccacg
     1201 ggcgaacgtg aacccaggac atctacatcg cattgataga actcgcgaaa acgtccgcgc
     1261 gccggccgat ccgcacgcca gactggctga atctgataac gcttgaaaaa ctttggcagc
     1321 ctgtcacggt actcggcgac aacacgggcc aggggaaccg tcaggtcgta acgcaatgcc
     1381 aggtcggcct cgcctgtggc ctcatgcaca ccgcgtttca ggatcttgaa aattaactgg
     1441 ttaccctctt caccatactt acccaacagc gtttcgatgt tttcaaccgc cggtgtttca
     1501 agcggctcaa agccataacg ctcatacacc tctttgatca caccgatgac gtattcgcgg
     1561 cgtcgcacgt ccgcgggaag gaaatcacgc attccacgcg cgggttgggt cttagctctc
     1621 gacatcactt gaagattgaa aaacccccgt agaccacaaa ttgatttaac cctcgattgg
     1681 agccggatcg atcgccattc gagatgtggt gaaagcgaac accgagcgtg atcgcacggg
     1741 tttctgatgt gaagatgcgc acaccgctgc cggcttctcc aacaaaggca aaacgacctg
     1801 cgtcgggaag cggcactgat ttgctgaaaa tcagaccacc gccgttaaca tgaacaaacg
     1861 ggtgaacgcg agagccattc ataaaatcaa gctgcagccc caaaggcgtc agtccaccac
     1921 cgtaggtggt ttctcgcgtg aaagtcgtta ccggaccgac agttgttacg gaggtgatgt
     1981 ttcctatcgc aagctctatt ggaatggcat ccagcgtgta ctggagcgcc atgctgtcat
     2041 tcgccgccag ggtcctgcca tagcgaaatg ccgccatgaa aagtccgcgg tcccttgctt
     2101 cgtcatcgtg caatccgccg aaaatcgttg tcgcctggaa tgcaccgcct gcccaaaaac
     2161 cgaattcatt atgccctttt cgtaacgtat agggctcgtc tcccttggtc tgaggaagtg
     2221 cttcagtagt gccctcgact cccgtatcgc tcacggaggg tgtggtggcc tcatcgagct
     2281 gtggccgtgg ctgttgagcg cacactgtga acgcgatgtt gagaattaca agaatcgttg
     2341 ccgcgaaaat gcgacggtaa gtgtccatct gtttcgtggt cccccaaggg tatctaacgc
     2401 tcttcgttgt acatcctcgt gtattcaacg taattctgcc aaaagacatc gagaccgctg
     2461 acctcctcag ccgtcagcgt tcgtttcacc cttgccggta ttcccatcac cagcgagtgc
     2521 gggggaatga ttgtgccggg tgaaactatc gccccggccg caacaatcga atactcaccc
     2581 acccgcacgt cgtccagcac gatcgatccg attccaatca gcgatccctt ttccacatag
     2641 catccgtgca gagttacgct gtgcccgacc gtgacctggt cctcaatgat cgtggggtgt
     2701 gaataatgac ggacgtggat tacggaattg tcctgaatgt tggtgcgatt tccaatccta
     2761 atgaagtgta cgtcgcctct gatgactgaa ttgaaccaga cggaagagtc ttcacctaga
     2821 tgtacatcgc caattacgca ggcactttcc tcgacaaaag cgctggggtg aatctgcggg
     2881 tgaactccgc ggaacggtcg tatcatagtt tctgcaatac tagcaggatt gcggaatgaa
     2941 tgtcagatag gtttcgcaaa aacagacgaa aacctgtgca agacgagtgc agtatccaca
     3001 acctgggcct gcgctcaatt gcgttgacat aaaacggacc gccgtgagag attgcgcgag
     3061 tgactcgacg aatgacttcc cgattgcgat tcctc
//
LOCUS       DASUEX010000537         3010 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_430557, whole genome shotgun sequence.
ACCESSION   DASUEX010000537 DASUEX010000000
VERSION     DASUEX010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430557"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="VFV61_09020"
     CDS             complement(<1..268)
                     /locus_tag="VFV61_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900661.1"
                     /translation="MKLTRTRMHRAMGVFTIMAAASLVASLSALPVHAQPASSSGQFM
                     ELMKMRQELSSRAIEETRRRRFEEGKSDSNFPSDSFGRKGGVVPA"
     gene            complement(473..1861)
                     /locus_tag="VFV61_09025"
     CDS             complement(473..1861)
                     /locus_tag="VFV61_09025"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX4900662.1"
                     /translation="MKALTDQMIRVSSALTLSLLLTAAVLAQSQLAAASASPQSKTPI
                     RRTTATRPQTKATPQTSPDPTPAATPSTVPASEAPAATPAATEVLEVAKEETPQPVKL
                     DPVLSQETRLRQQLDVVEQMVAQGLKQEALAELRSVAAEDRFDPQGFYNIANAFARLD
                     ATDEAISAYRKALEQRKGHYSRAANNLGVVLLRQGFWDQAYEAFLLALRTENFHYAEA
                     SYNLGRLYAARGEMDRAVREWRRAVTVNPNHVAAAQALGSATPKFSSTSSGTDGGGVA
                     TQAANASSHSSRPIFTLDTETHELLQQARNAHENRRYADAVEGFKRVIARQRGYFAPA
                     TLELSYSLMELGRNEEALAALLLVAEKDAARFPITYYHLGRLYERRGDLQHAEENYLR
                     ATQAHQVENAQFLLNLSSVREKRGDYVGALMAMENYVKRKAQQGQKPEWSDARLKELR
                     DKAASGTSPTKP"
     gene            complement(1858..>3010)
                     /locus_tag="VFV61_09030"
     CDS             complement(1858..>3010)
                     /locus_tag="VFV61_09030"
                     /inference="COORDINATES: protein motif:HMM:NF026220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HEX4900663.1"
                     /translation="RDPSLEPGSCKHDVGNLQSAIGNHVMGRETKQQIGNRQSPIGNS
                     RCCRLFFAACLLLLTISPSALAQSGAWARQTSGSLSWLHGVFFLDQNRGWAVGSKGAL
                     LATEDGGKSWKARPKPSDDVLRDIYFADPLNGWIVCERNIYQLKSNTDPRTYLMNTTD
                     GGGNWQRVVLPGADVNARLVRAVFSPGGSGWAFGEFGAVYKSSGGDAKWVRLQAPTRH
                     LLLGGTFIDDNRGWLVGAGATILQTSDGGETWHVSQLADAPGIRFNATSFVGNRLGWA
                     VGSGGSIYHTVNGGRTWQPQRSGVSVDLYDVKFLDAVEGWAVGAEGTAMYTADGGLNW
                     VTEPSNTTHPLERLFFADRTHGWAVGFGGTILSYVRAEAPRLHRNTSQQ"
BASE COUNT          611 a          863 c          877 g          659 t
ORIGIN      
        1 gtgcaggtac aacgccaccc tttctgccaa aactgtccga ggggaagttg ctgtccgact
       61 ttccctcttc gaagcggcgc ctccttgtct cctcaatggc gcggctggat agctcctgcc
      121 gcatcttcat gagctccatg aactgccctg aagaggaagc tggttgcgcg tgcaccggga
      181 gtgctgagag actggccacc aacgaggccg cggccataat ggtgaagaca cccatggcgc
      241 ggtgcattct agttctcgtc aacttcatgg agagcctcct gcggcaatgc gttgcagctt
      301 ctgatcccgt ggctcggagg agtcctcaag tctgggcggt tcgattatac gccggactaa
      361 gcctacgttc gaactaccgt cgcggccgat gacaactttc agagtttatt tgactgatca
      421 cagatcacca atcgacgatc gacaatcgac aatcgacaat cggcaatcag gactacggct
      481 tggtggggct tgttcctgag gctgccttat ccctcaactc cttgagccgc gcgtctgacc
      541 attcgggttt ttggccctgc tgtgctttgc gtttgacgta attctccatc gccatcaggg
      601 ccccgacgta gtcgccacgc ttttcgcgca cgctgctgag gttcagtagg aattgcgcat
      661 tctcaacttg atgcgcctgg gtagcgcgca aatagttttc ttccgcatgc tgcaagtcgc
      721 cccgccgttc gtatagccgg cccaaatggt aataagtgat gggaaagcga gccgcgtctt
      781 tctcagcgac gagcaagagc gctgcaagcg cttcttcgtt gcgtcccagc tccatcaggg
      841 aataactaag ttccagggtg gcaggagcga aataacctcg ctggcgagca atgacgcgct
      901 taaaaccctc aactgcgtcc gcgtagcggc gattctcatg agcgttgcgc gcctgttgca
      961 gcaactcatg tgtttcggta tcgagtgtga aaatgggcct ggacgagtga gacgaggcgt
     1021 tggccgcttg tgtcgcaacc ccaccaccat ccgttccact cgaggtgcta ctgaattttg
     1081 gggtggcact acccagggcc tgggccgcag caacatgatt gggattaact gtgacggcgc
     1141 ggcgccattc gcgaacggca cgatccattt caccgcgtgc agcatataag cggccgaggt
     1201 tgtagctcgc ttcggcataa tgaaagttct cagtgcgcaa cgctaataga aaagcctcgt
     1261 aggcctgatc ccaaaaaccc tgacgcaaga ggactacgcc aagattattt gcagcacgtg
     1321 agtagtgacc cttccgctgt tcaagtgctt tccggtacgc gctgatcgcc tcgtcagtag
     1381 catcgaggcg ggcaaatgca tttgcgatgt tatagaagcc ctgcgggtcg aaacgatcct
     1441 ctgcagcgac tgagcgcagc tcagccagcg cctcttgttt taagccctgc gccaccattt
     1501 gctccacaac atccaactgc tggcgcaatc ttgtttcctg gctgagtacc ggatccagct
     1561 tcactggttg cggcgtttct tctttagcga cttcaagaac ttccgtagcc gctggagtag
     1621 ctgccggagc ttcagacgct gggacggttg acggagtggc cgccggcgta ggatcaggtg
     1681 aggtctgcgg agtcgccttg gtctggggcc gagtcgccgt agtgcgtctg atcggcgttt
     1741 tggattgtgg ggaagctgaa gcggccgcga gctggctttg ggccagaaca gctgcggtca
     1801 gcagcaggct aagtgtaagt gccgaggaaa ctctgatcat ctgatctgta agggctttca
     1861 ttgctgtgag gtattacggt gcaggcgtgg cgcctcggct ctgacgtagc tcaagattgt
     1921 gcctccaaag cctactgccc aaccatgggt acggtccgcg aaaaacagcc gctccaacgg
     1981 atgagtggtg ttgcttggct ccgtgaccca attcaagccg ccgtccgccg tgtacatagc
     2041 tgttccctcg gcgcccactg cccagccttc aaccgcgtca agaaatttga cgtcatagag
     2101 atctaccgaa acaccggagc gctgcggttg ccaggtcctg ccgccattga cggtgtgata
     2161 gatactaccg ccgctgccaa cggcccaacc caggcgattt cctacgaacg aggttgcatt
     2221 gaagcgaatg ccgggcgcat ccgcgagttg tgagacgtgc caggtttcac caccatcgga
     2281 agtttgcaga atcgtggccc cagccccgac taaccagccg cggttgtcgt cgatgaacgt
     2341 accacccagt aagagatggc gcgttggggc ttgcaggcga acccatttgg cgtcgccccc
     2401 cgacgactta tagaccgcgc cgaattcacc aaaggcccag ccgctgccgc cggggctgaa
     2461 gacggcgcgc acgagccggg cattgacgtc agcgccgggg agcaccacgc gttgccaatt
     2521 cccgccaccg tcggtggtgt tcattagata ggtgcgcggg tctgtattgg acttcaactg
     2581 gtagatgttg cgctcgcaaa cgatccagcc attgagcggg tctgcaaaat aaatatcgcg
     2641 taagacatcg tcgctcggtt taggacgagc cttccaggat ttgcctccat cctcagtggc
     2701 aagcaatgcg cccttactgc caacggccca accgcgattt tgatcgagga agaaaactcc
     2761 gtgcagccag gacaacgagc cggaggtctg gcgcgcccag gcgccgcttt gcgctaatgc
     2821 tgatggggag atagtgagca gcaggagaca ggcggcgaag aacagacggc agcagcgcga
     2881 attgccgatt ggtgattgcc gattgccgat ttgttgcttc gtctcgcgac ccattacgtg
     2941 attgccaatt gctgattgga gattgccgac gtcgtgcttg catgagccgg gttccaggct
     3001 tggatccctg
//
LOCUS       DASUEX010000538         3548 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1475450, whole genome shotgun sequence.
ACCESSION   DASUEX010000538 DASUEX010000000
VERSION     DASUEX010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1475450"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(135..971)
                     /locus_tag="VFV61_09035"
     CDS             complement(135..971)
                     /locus_tag="VFV61_09035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900664.1"
                     /translation="MKNLLSILRNAALSVAFTLVFVAAPAAQTANVSAPATQKSNDAK
                     AETIINSAVAALGGDAYLNVRTVVGRGFFTTYQDGASLVPTRFLDYIVFPDKERTEFT
                     TQGIRVINTNNGDTGWLYDGATKSLNDLKPAQIEDFKRAMRTSVENLLRGWWRKENAT
                     LAYVGRREAGLAKRNETVRITYPDGFWVEFEFGAKDALPAKVIYKRTRTKADSDETEE
                     VTEEDWLAKPITNVGITAPFVIDHYLNGKQTSRINYESIEYNRSLPDTLFAKPANAKS
                     VK"
     gene            complement(1057..3342)
                     /locus_tag="VFV61_09040"
     CDS             complement(1057..3342)
                     /locus_tag="VFV61_09040"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEX4900665.1"
                     /translation="MVDLGAYKDKFGESGQRILEHALNESRQRDQNYVAVEHILEALM
                     HEEPDMFNSAMRELSLDPITVKIAIDKRLETSRQQHVGKGFRIAPDTTDLFKRAMERA
                     RAQGRKTIEATDIFEALTNYDSLFVKVLENMGANPDLVAENVRAQVQKREQEEEQYRK
                     KFELPPYLKHFGVSLNRLARADKIAPTIGREKEIQQMVEILCHRERANSPMLVGEPGV
                     GKTAVVEGLARLIELEPEKVPARLRGSHVVQLQMGGLVAGTMLRGMFEERIKGIIDEA
                     KEHANLILFIDEAHTIIGAGAALGTSSDAANMFKSALARGDMRIIGATTLSEYKEYIA
                     EDEALARRFRLVKVVEPSISETREILLGVRTRLERNYSVKISDDAINTALEMAPKYIR
                     NLHLPDKAIGWLDTAAVKVEINEPQTMVVKPEHVIDVISQESRIPRDMIFRDTGDRFA
                     LTEAMLGARVIGQKSAIKAVAQRLRLNKGPLKDSHYKPDGVLLFLGPTGVGKTELAKA
                     VAEFMFGDEEKMVRIDMSEYSDGTVSIEKLIGMPRGIVGSERGGILTEQLRENPYTVL
                     LLDEVEKASPYLMNLFLQAFDEGWLTDGRGKKVYLSDAIVIMTSNLGSDSFKRFEKPL
                     GFGTKTANDFRAVNNEVMKAAEQRFSPEFRNRIDEIIVFAPLTMDEVRRIARLYLDKL
                     SRQMERQGKSIDVTEAALNLLTEKGYSTAYGARFLKRHIDEKVKLPITSMWKTASSFL
                     VDVENGETVVKPAAPNKPELN"
BASE COUNT          754 a          989 c          921 g          884 t
ORIGIN      
        1 cgcggatcac ttcatctgct tcgtccggat cctgggcaaa agcaactggc ggactgaaag
       61 cgataactac taacaacaac aagaatggcg acagacgcag catggtattt cgagcgaggt
      121 ttcgcggctc tgatttactt cacgctcttg gcgtttgccg gcttggcgaa gagagtgtct
      181 gggagggaac gattatattc gatggattcg tagttgatcc ggcttgtttg tttgccgttc
      241 aggtaatgat caataacgaa gggcgcggtg attcctacgt ttgtgatcgg cttggcgagc
      301 cagtcttctt cagtgacctc ttcggtctcg tcggaatcag ccttcgtgcg cgttcgtttg
      361 tagatcactt tggcaggaag cgcgtccttc gctccaaact caaactcaac ccagaaacca
      421 tcggggtaag tgatgcgcac ggtctcgttt cttttagcta gtccggcttc acgtcttccc
      481 acataagcca gcgtggcgtt ttcttttcgc caccaaccgc gcaatagatt ttccacgctt
      541 gtccgcatcg cgcgtttgaa gtcctcgatc tgggcgggct tcaaatcgtt caaactcttc
      601 gtggcgccat cgtagagcca tccagtgtcg ccgttgttgg tgttgataac gcggatgccc
      661 tgggttgtaa actccgtccg ttccttatcc gggaacacga tgtagtccaa aaatctcgtc
      721 ggcacgaggg aggcgccatc ctggtaggtt gtaaaaaaac cgcgacccac tacggtgcgc
      781 acattcaggt aagcatcacc gccaagggcg gcgacggcac tgttgatgat tgtttctgcc
      841 ttcgcgtcgt tggatttctg ggtggcaggc gcgctaacgt ttgccgtctg cgccgcaggc
      901 gccgcgacaa aaaccagcgt gaatgcaacg ctcaaagctg cgttgcgtaa gattgacaac
      961 aaattcttca tggcactgat gagactttaa cgcacttcgc aaaaaagggt ttcaatgaaa
     1021 aatcactgaa accctgtttg acctctgggg aataaattag tttagctcag gcttatttgg
     1081 cgccgccggt ttcacgacag tttcaccatt ttccacgtcg accaaaaagc tggatgcggt
     1141 cttccacatg ctcgtgatcg gaagcttgac cttctcgtcg atgtgtcgct tcaaaaagcg
     1201 cgcaccatat gcggtgctgt aacctttctc ggtcaggagg ttcagagcag cctcggtgac
     1261 gtcgatcgac ttaccttgcc gttccatctg ccggctcaat ttgtcgagat aaagcctggc
     1321 aatgcgccgc acttcatcca tggtaagcgg ggcgaaaaca ataatctcgt cgatgcgatt
     1381 acgaaactcc ggcgagaagc gctgttccgc ggccttcatg acctcgttat tgacggcgcg
     1441 aaagtcgttg gctgtctttg tcccaaaccc cagcggcttt tcaaatctct tgaagctgtc
     1501 ggaaccgaga ttggacgtca tgatcacaat ggcatcagac aagtaaactt tcttgccgcg
     1561 accatccgtg agccaaccct catcgaaggc ttgcaggaac aagttcatca agtaaggcga
     1621 cgccttctct acttcatcca gcagcaggac tgtgtaggga ttctcgcgca gctgttcagt
     1681 gagaatgcca ccgcgttcgg aaccgacaat gccgcgcggc ataccgatca gtttttcgat
     1741 gctgaccgtg ccgtcgctat attccgacat gtcgatgcgg accatctttt cttcgtcacc
     1801 aaacatgaac tcggcaacgg ccttggcaag ttccgttttg ccgactccgg tgggacccag
     1861 gaataagagc acgccgtcgg gtttatagtg cgaatcctta agcggtcctt tgttcaagcg
     1921 caaccgctgg gcgactgcct tgatggcgct cttttgtccg atcacgcggg cgcccagcat
     1981 cgcttccgtt aacgcgaaac gatcgccggt atcgcggaaa atcatgtcgc gcggaatccg
     2041 cgactcctgg gaaatcacat caataacgtg ttcaggcttg accaccattg tctgcggttc
     2101 attgatctca accttcaccg ctgcggtatc gagccagcca atcgctttgt ccggcaggtg
     2161 aagattgcgg atgtacttgg gcgccatttc gagagcggtg ttgatggcgt cgtcggagat
     2221 ctttacagag tagttacgct cgaggcgggt gcggacgccg agcagaatct ctcgagtttc
     2281 actgattgat ggctctacaa ctttgacgag ccggaagcgc cgggccaggg cttcgtcttc
     2341 ggcaatatat tccttgtact cggagagcgt ggtggcgccg ataattcgca tgtcaccgcg
     2401 cgccagcgcc gacttgaaca tgtttgcggc atcagacgat gtgcccaaag ctgccccggc
     2461 gccgatgatc gtatgcgctt catcgatgaa caggatcagg ttggcatgtt cctttgcctc
     2521 atcgataatt cctttgattc gctcttcgaa catgccgcgc agcatcgtgc cggcaacgag
     2581 gccacccatt tgcagttgta cgacgtggga cccacgcaag cgcgccggaa ccttctccgg
     2641 ttccaattca atcaggcgtg ccagaccttc caccaccgca gtcttgccga cccccggttc
     2701 gcccaccagc atcggtgaat tcgcacgttc gcgatgacag aggatctcaa ccatctgttg
     2761 aatttctttt tcgcgaccga ttgtgggagc gatcttgtcc gctcgggcta atcggttcag
     2821 ggaaacgcca aagtgcttta agtaaggagg cagctcgaat ttcttgcgat actgttcttc
     2881 ttcctgctcc cgtttctgca cctgcgcgcg cacattctcg gcgacgagat ccggattggc
     2941 gcccatgttc tcaagcactt tgacgaaaag actgtcgtag tttgtcagag cctcgaagat
     3001 atccgtcgcc tcaatcgttt tacggccctg ggcccgcgcg cgttccattg cgcgcttgaa
     3061 gaggtcggtc gtgtcaggag cgatccggaa gcctttaccc acatgctgct gccggctggt
     3121 ttccagccgc ttgtcgatgg caatctttac ggtgatcggg tcaagagaga gttcgcgcat
     3181 cgcggaatta aacatatccg gctcttcatg cataagagcc tccaaaatat gttcaaccgc
     3241 aacataattt tggtcccgtt ggcgcgactc gttcagggca tgttccaaaa tgcgctgacc
     3301 gctctctcca aacttatctt tgtatgctcc aagatcaacc attgtgtacc acctgctgcc
     3361 ctcagtaact ctcttaacaa acaacggata ggccgtgtgt tttgttcagc gagactgtca
     3421 tcgatcgttc aaaataacga catccaaccc gagggttaga tgcaactgag catatgcgcc
     3481 gttgtgtttc aatgcaatac ctatctcccg gctcgaccgc ttgcaaatct tcggccagcg
     3541 gcccagac
//
LOCUS       DASUEX010000539         2329 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_96580, whole genome shotgun sequence.
ACCESSION   DASUEX010000539 DASUEX010000000
VERSION     DASUEX010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2329
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96580"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1064)
                     /locus_tag="VFV61_09045"
     CDS             complement(<1..1064)
                     /locus_tag="VFV61_09045"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX4900666.1"
                     /translation="MAKILILGGGFGGVVAAERLAEQLGDDHQLTLVSRSRNFVFYPG
                     LVRLAFGKCQPEDVSFNLRESILSRRVNFIEAEVARIDPIERKVIIAHGEVEGKLNYD
                     YLVFALGRRLASERVTGFYEHAHHLLNVDKAIKFGKALERFHEGRAVIGQCPDARLPV
                     PVYETAFALSRLLQERGEREQVRITVVSPDPPDLQLDDPQASIALESAFASHGIELVA
                     DFPINRLTPGAAITSTGLVMNFDLLMLVPPFQGSPTVLHLGMTDQAYINVDSTMRVIG
                     ADRMYAVGDCVNFSGPKMGHMAVRQAEVAAANLAAEVEGHAAVSHYSHEMKLVVDAGG
                     CDSIYLHKDIWTNEPASVRQG"
     gene            1346..2089
                     /locus_tag="VFV61_09050"
     CDS             1346..2089
                     /locus_tag="VFV61_09050"
                     /inference="COORDINATES: protein motif:HMM:NF012350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HEX4900667.1"
                     /translation="MNETTLPLSEAQTSVSRRGGVANRTLQWHALDGASVARLLEVDP
                     SQGLTASEVATRRLRYGSNSSDTLRRRNGLVLLLFLSITVGFLLTAAVLNRAITDQVE
                     AFSILAVLAIMAIVGFAYLLQGAHELDAVQDATRSKVRVRRDRQETKVIAEELVPGDI
                     VVLEAGDCVSADARLIKAEQLQVQESALTGNIANVEKSVSQVASEAPLEMRHSMVYLG
                     TTISSGSGVGVVVATGLATEVGKIAARDN"
     gene            2091..>2329
                     /locus_tag="VFV61_09055"
     CDS             2091..>2329
                     /locus_tag="VFV61_09055"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX4900668.1"
                     /translation="MNSTTNSKAPAIEFRNVSLSFNSQPVLVDISFTLEQGEMIFLTG
                     VSGSGKSVLLRLAMGLLKPDAGHIFVEGREIETLA"
BASE COUNT          529 a          656 c          611 g          533 t
ORIGIN      
        1 ccttgcctaa cgctcgccgg ctcgttagtc caaatgtcct tgtgcaggta aatgctgtcg
       61 caaccaccag cgtcaactac cagtttcatc tcatgactat agtgtgaaac ggccgcatgt
      121 ccctcgactt cagcagcgag atttgccgca gcaacctctg cctggcggac tgccatatgt
      181 cccatcttcg ggccgctaaa gttcacgcaa tcaccaaccg cgtacattcg gtcggcacca
      241 atcactcgca tagtcgaatc gacgttgatg tatgcctgat cggtcattcc taaatgcaat
      301 actgtcgggg atccctgaaa tggcggcact agcattagca ggtcgaagtt catcaccagt
      361 ccggtgctgg tgatcgccgc acccggagtc aacctattaa tcgggaagtc tgccaccaac
      421 tctatcccat gggaggcgaa ggcactctcc agcgcaatcg aggcctgggg atcgtcgagt
      481 tgcaaatccg gagggtcggg actgacgact gtgattctta cctgctctcg ttcgccgcgc
      541 tcttgcaaca atcgcgacaa cgcgaacgcg gtttcgtaaa caggcaccgg cagtcgcgca
      601 tccggacact ggccgataac ggctcgtccc tcatggaacc gttccagagc tttgccaaac
      661 tttattgctt tatcgacgtt gaggagatgg tgtgcgtgct cataaaaacc cgtgacgcgt
      721 tccgaggcca gccgccggcc gagagcgaag accaaataat cgtaattaag ctttccttca
      781 acttcgccgt gcgcaatgat tactttgcgt tcaatgggat caatgcgtgc tacttcagct
      841 tcgatgaagt tcacgcgccg actaaggatg ctctcgcgta gattgaagga aacatcttca
      901 ggctgacact taccgaaggc caatctcacc agccccggat agaagacaaa attccgcgag
      961 cgcgaaacca atgtgagctg atggtcgtcg cccaactgtt cagctaaacg ttcagcagca
     1021 acaaccccgc cgaagccgcc tcccagaatt aagatcttcg ccatttgccc aacctcaaca
     1081 atcaacctga tctttgacgc cacttaacgt catgcttctc tcggtgcttt gcacgggccg
     1141 taccagttac gccccccgaa gttttcggcg aggttttggc gcacacgtct ggttttgcag
     1201 ccaatagctg cggggtattg cgcaacgaag ctgaattttc gcccaacaca gcaaaacctc
     1261 gcttcagatc ttttagaaaa gcggcaaatt ctcgggctcc caaggctcca gcggcggcac
     1321 acacgttgct ccttagggtt gcatgatgaa tgaaacaact cttccactaa gcgaagcgca
     1381 aacgagcgta agcagacgcg gcggggtcgc caaccgtact ctgcaatggc acgctctgga
     1441 tggagcatct gtggctcgcc tgctcgaggt tgatccttcc cagggattaa cagcttcgga
     1501 ggttgccact cgtcggctcc gttatggatc aaatagcagc gataccttgc ggcgccggaa
     1561 cggacttgtc ctgctgctgt tcttaagcat caccgttggc tttttattga cggctgcggt
     1621 gctgaatcgc gcaataacgg accaagttga ggctttttca attctcgcag tcctggcaat
     1681 catggcgata gttggtttcg cctatttgct ccagggagca cacgagctgg atgcggtcca
     1741 ggatgcgaca cgaagcaaag tccgggtaag gcgcgatcgt caggaaacca aagttattgc
     1801 tgaagagctt gtgcccggtg acattgtcgt cctcgaggcc ggagactgtg tctccgctga
     1861 tgctcgactt atcaaggctg aacaactgca agttcaggaa tcggccttga cgggaaacat
     1921 tgccaacgtc gaaaagtctg tttcacaagt tgccagcgag gcgcccctgg aaatgcgcca
     1981 ctcgatggtc tatctcggca ccacgattag ctccgggagc ggtgttgggg ttgtcgttgc
     2041 caccggcctg gcgacggaag tcggaaagat tgcggctcgt gacaactgag atgaactcga
     2101 caaccaacag caaggcgcca gcaattgagt ttcgcaacgt ttccctgagc ttcaatagtc
     2161 aaccggtcct ggttgatatt agtttcacac tcgaacaggg tgagatgatc ttcctgaccg
     2221 gcgtctctgg ctcgggaaaa tctgtcctct tacgtttggc catgggtctg ctcaagccgg
     2281 acgcgggcca tatttttgtt gagggtcgcg aaattgagac acttgccga
//
LOCUS       DASUEX010000540         3486 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1234601, whole genome shotgun sequence.
ACCESSION   DASUEX010000540 DASUEX010000000
VERSION     DASUEX010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3486
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1234601"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(26..601)
                     /locus_tag="VFV61_09060"
     CDS             complement(26..601)
                     /locus_tag="VFV61_09060"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX4900669.1"
                     /translation="MSDELLLERAKGGDQAAFDELYERHRNAVFRFAYRLLGSVETAE
                     DVTHDCFLGLIRRPGGFEPSRSGFRTYLYAAARNLCLKHFRRSGRPGSFEELTEEPTG
                     PASEQPLHRLLDGELSKKVREAVMGLPPLQREALVLFEYEGLALSEIADVVGSDIGAV
                     KARLHRAREGLRTTLRPYLNSALEIATLEKP"
     gene            952..2376
                     /locus_tag="VFV61_09065"
     CDS             952..2376
                     /locus_tag="VFV61_09065"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEX4900670.1"
                     /translation="MKLISRNTSATLPQKVAFLVLAALLVTPLLARAQFTQQPEREQM
                     LNGLRLLLVHKPGDGDVFLSLRIHSGAAFDVEGKAGEMAVLGDILFPDPTTREYFTDE
                     MAGRLDVDTDHDSITINMKGRADQFERIVEILRTALVTTQITPENVARIRDGRIKIAK
                     ETVIAPDVLADRAIAARLFGDFPYGRPASGSAESLSRVDRADLLLARERFLNANNATL
                     VIMGGVQRSRAVRALRQLLGPWRKSEQIIPTTFRQPTAPDVRPLVINAASDQSAEIRL
                     AARGLARSDRDFNTVNLLAIVARGRWQKLSPENARRPLFVRHEAHVLPGMFVMGAAVD
                     TLLVAKTLTQAREAIQSLILVPATVDELARAKAELNAQVAKDLTLPEGIARAWLDADT
                     FGLSTSAVEPLQIDAITPADLQRVANRLFKDAPVATIIIGDSKQLKAALEPSIKVEMM
                     GEIAPRPAKSETKPAMTIPIRKPE"
     gene            2433..3176
                     /gene="ubiE"
                     /locus_tag="VFV61_09070"
     CDS             2433..3176
                     /gene="ubiE"
                     /locus_tag="VFV61_09070"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: protein motif:HMM:NF001244.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HEX4900671.1"
                     /translation="MSDTRKAIEDDHASRVREMFATIAGRYDLLNHLLSGQMDKRWRR
                     LVASRLGERLGSNEALILDVACGTGDLSLQLFETIGVRVVATDFCRPMLDIAARKVAK
                     AMADNRLASIPLIEGDALRLPFRDGSFDGVTIAFGLRNLSNVERGLAELFRVLKPGGS
                     LAVLEFSKPVVPGFSFLFKAYFTKLLPFVGGLISGSRSAYQYLPDSVSRFPDQKELVA
                     RMQQVGFERVEYQNLTGGIAALHLATRPH"
BASE COUNT          773 a          959 c          924 g          830 t
ORIGIN      
        1 tgatctccgg gtccgatgct gctcattacg gcttctccaa agtagcaatt tcaagggcac
       61 tattcaaata gggtcgcaaa gtggtgcgta gcccttcgcg tgctcgatga agccgggcct
      121 tcaccgcccc aatgtcactg ccgacaacgt cggcaatctc acttaaggcc aacccttcat
      181 attcaaagag aaccagcgcc tcgcgctgta gcggcggcag gcccatgacc gcttctctaa
      241 cctttttgga gagctcgcca tccagcaacc ggtgtaaagg ctgttcgctg gccggccctg
      301 tcggctcctc agtcagctcc tcaaacgaac cgggtcgccc cgaccggcga aagtgtttca
      361 aacataagtt ccgcgccgcg gcgtagagat aggtgcgaaa cccactgcgt gacggttcaa
      421 agcctcctgg ccgtcgaatc agacccaaaa agcaatcgtg cgtcacatcc tcagccgttt
      481 caacggaccc cagcagccgg taagcaaacc gaaaaactgc gttccgatga cgttcgtaca
      541 gctcgtcgaa agctgcctga tcgccgcctt ttgccctttc aagtaagagc tcgtctgaca
      601 ttctctctgg caatcaagct ccaatctctt aagcaagcct gagggaagcg cttcctacct
      661 ttattaccgc ccaaccccca aaagatacgg ccttccacca acttttttgg atttctgcgc
      721 cgcgggactc aaacaacacc taatatgttt tcgaccattg ccttccgcat gacacccaaa
      781 tagcaggtaa cggcatctga tcgacaagcc tatgatgcat ttacgggcca aacggttgac
      841 gcactaatat cgggcatcta agatggcctg gctcccctaa attctgtgaa cgtcagagtt
      901 aagacgggca agtttgatag ttcaggggta ttcatcaaga tcgttgcatt tatgaagtta
      961 atctccagaa atacctccgc aacgctaccg caaaaggtag cttttcttgt tttggcagct
     1021 ctccttgtga cgccgctttt ggcgcgcgct cagttcactc agcaacccga acgcgagcaa
     1081 atgctgaacg gcctccgctt gctactcgtc cacaaacccg gggacggcga cgtattcctg
     1141 agtctccgca tccatagcgg cgctgccttt gatgttgaag gtaaggccgg cgaaatggct
     1201 gttctgggcg acattctctt tccagacccg actactcgtg aatacttcac ggacgagatg
     1261 gccggccgcc tcgacgtcga caccgatcac gactcgatta ccataaacat gaagggtcgc
     1321 gccgatcagt ttgagcgaat tgtggaaatt ctgcggactg ccctggtaac tactcagata
     1381 actcctgaga atgtcgcgcg gattcgtgat ggtcggataa agattgccaa ggaaacggtg
     1441 atcgctcctg atgtgcttgc tgatcgggcc attgcggctc gtctatttgg tgactttccc
     1501 tatggacggc ccgctagcgg atccgccgaa agtctcagcc gcgtagatcg cgcggatctt
     1561 ctactcgcgc gagaacgctt ccttaatgcc aataatgcga cgctggtaat catgggtggg
     1621 gttcaacgca gccgagcggt cagagctctg cgacagctgc tcggcccctg gcgtaagagc
     1681 gagcaaatca tccctacaac tttccgtcag cctaccgccc cggatgtgcg tcccctggtc
     1741 atcaatgctg cttctgatca gagtgccgaa atccgtctcg ctgctcgcgg gttagctcgt
     1801 tctgatcgag acttcaatac agtgaacctc ctggcgatag tggcacgtgg gcgctggcaa
     1861 aaattgtcac ctgaaaatgc gcggcgccct ctctttgtaa ggcatgaggc acacgtgctg
     1921 ccggggatgt tcgtgatggg cgcggcagtg gatactttgc tcgtggcaaa gacgctgact
     1981 caggcgcgtg aagcaattca gtcgctcatt ctcgttccgg ccaccgttga tgaacttgcg
     2041 cgggccaaag cagaactaaa tgcgcaggtc gctaaagatc tgacgcttcc ggaagggatc
     2101 gcacgagctt ggcttgatgc ggatactttt gggctatcga ccagcgcagt cgagccgctt
     2161 caaatcgatg cgattacacc cgcggatctg caacgagtag ctaaccggct cttcaaggat
     2221 gcgccggtgg cgacgatcat catcggcgat tccaaacaat taaaggctgc actggagccc
     2281 agtattaaag ttgaaatgat gggcgagata gcgccgcgtc ctgccaaatc tgaaaccaaa
     2341 cccgcgatga ccattccgat caggaaaccg gagtaatcgc aaatctttcg caatctgctt
     2401 gcaaccatac ctcaatcgaa tttcgcttaa ctgtgtctga cactcgcaag gcaattgaag
     2461 acgaccacgc gagtcgcgtc cgcgagatgt tcgcgacgat cgccgggcgc tacgatttgc
     2521 tcaatcatct gctctccgga cagatggaca agcgttggcg taggttggtt gcgagccggc
     2581 taggcgaaag acttggatcg aacgaagcgt taattctcga tgttgcctgt ggcacgggcg
     2641 atctttcgct gcagttgttt gaaacgatcg gggtgcgggt agtggccaca gatttttgta
     2701 ggccgatgtt ggacatcgcc gcgcggaaag ttgcgaaggc aatggcagac aatcggctgg
     2761 catcaatccc gctaatcgaa ggcgacgcct tacgcttgcc atttcgcgat ggctctttcg
     2821 acggtgtgac catcgcgttt ggcttacgca atctttcgaa tgttgagcgg ggactcgccg
     2881 aattgtttcg ggtgttaaag ccggggggca gtctcgcggt cttagagttt tcaaaacccg
     2941 ttgttcctgg gtttagtttt ctgttcaaag cctatttcac aaagctgctc cctttcgttg
     3001 gtggtttgat tagcgggtcg cggagcgcct atcagtactt gccggattcc gtttcgcggt
     3061 ttcctgatca gaaggagcta gtggcgcgga tgcagcaagt cggctttgag cgagttgaat
     3121 atcagaatct aacgggcgga atagcagcgc tacatttagc cacgcgcccg cattgattta
     3181 ttgagattgg ttgcaactaa cagttgtggc cagtagagag cgcagccggg atatagcctc
     3241 ttgagcgtgc ggttgtcggg gccatgcgac tcattctgag gtttaattcc tggctgcgct
     3301 cttgtttgcc attacttcct accaaagatc tcttttctgc gtcacaaggc taagggaacg
     3361 atcggatgat atcggtccga tgccaatttg ctgtgccgtg ttccctaacc tcgcaatagg
     3421 caatgcgcaa ttggcaatcg acaatcgcaa attccgtcga tcgtttgcta ttatcctcat
     3481 gaattc
//
LOCUS       DASUEX010000541         2537 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1142884, whole genome shotgun sequence.
ACCESSION   DASUEX010000541 DASUEX010000000
VERSION     DASUEX010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2537
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1142884"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1534
                     /locus_tag="VFV61_09075"
     CDS             <1..1534
                     /locus_tag="VFV61_09075"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF010588.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HEX4900672.1"
                     /translation="LDWFLDRDVALHALEPLQQIIPATAGITAVVGTVRPTEKTSGRR
                     LYNSAAIIRDRQLLGFADKTLLPEYDVFDDPRYFEPATQRQLFQVDDSELGVAVCEDF
                     WNDKTFWKNRLYSHDPADELIEMGAQVLVSINASPFNKGKMGQRCSMVSHRAKAANLP
                     VVFVNLIGGNDGIIFDGASIVADCEGKIILQAPPFEEFIGTVDLKCGVRDERCLPGDG
                     IETVRSALVLGIRDYARKNRFSKAVIGLSGGLDSAVVAALACEALGPENVLSVMMPSP
                     YSSRSSIDDSVELGRRLGMHVIERPITEAFQVLLKELNLKPPSAAEKNTHAAENLQSR
                     LRGNILMSISNDEGRLLLSTGNKSELALGYCTLYGDTNGGLAVIGDVLKTEVYALARH
                     FNSAREIIPLSIINKRPSAELAPDQFDDQSLPPYDQLDPILRMYFEDKATPDEIVAAG
                     NSSELVYDILNRVESPANEFKRRQLPPTLIISRNAIGIGRRRPVTHHYKRRPAEKMQS
                     AD"
     gene            1801..>2537
                     /locus_tag="VFV61_09080"
     CDS             1801..>2537
                     /locus_tag="VFV61_09080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900673.1"
                     /translation="MQRFSYSSYLVAAVMVTIVVSSVVSAIAQQPSAQPKAPEAEVKA
                     AAAISSAPDLAAKLTQAEAFAKKYPKSTLRPQMVQGLTAEISRVTDAGQRLALAERFL
                     KAFSNDVESGYMKALVVDDYVRANRVDEAFSLGATTLAKQPENLGVLSALALAGTEEA
                     RKQNTKYATQASQYGVKAIELIETNKKPADMDDSFWNYQKLLLPKLYLSMGALAMAAG
                     KPAEAKPRLEKAIQVNPTEPTAYVFLGG"
BASE COUNT          595 a          660 c          711 g          571 t
ORIGIN      
        1 gctggattgg tttcttgatc gcgacgtggc gctccacgcc ctggagccgc tccagcaaat
       61 tattccggcg acggccggga tcaccgctgt tgtcggcacc gttaggccga cggaaaagac
      121 tagcggtcgg cgtctctata actccgccgc gatcattcgc gatcggcagt tgctcgggtt
      181 cgctgacaaa accctgctac ccgagtacga cgtctttgat gacccgcgtt acttcgagcc
      241 cgcaacgcaa cgacaactct ttcaagttga tgacagcgaa ctcggtgttg cggtttgtga
      301 agacttttgg aacgacaaga cgttttggaa aaaccgtctc tattctcacg atccggccga
      361 tgaattaatt gagatgggcg cacaggtgct ggtctcgatc aatgcttcac cttttaacaa
      421 ggggaagatg ggtcagcgtt gctcgatggt ctcgcatcgc gcgaaggccg ccaacctgcc
      481 agtcgtcttc gtcaacctga ttggcggcaa cgacggcatc atcttcgacg gcgccagcat
      541 cgtcgccgat tgcgagggca aaatcatttt gcaggcaccg ccttttgaag agtttatcgg
      601 caccgtcgat cttaagtgcg gcgtgcgcga cgagcgttgc ttgccgggag atggtattga
      661 gactgtcagg agcgcattgg tcctgggaat tcgcgactat gcgcgcaaga atcgcttttc
      721 aaaggcggtg attggcctgt ctggcggact cgattctgcg gtggttgccg cccttgcttg
      781 tgaagctctg ggtccagaaa acgtgctgtc agtgatgatg ccgtcgcctt actcgtcacg
      841 ttccagcatt gacgattcgg tagaactggg ccgacggctt ggcatgcatg tcattgaacg
      901 accgattacg gaagcgtttc aggtgttgct gaaagagttg aatctcaaac cacccagcgc
      961 cgccgagaag aacacccacg ctgcggagaa tctgcagtcg cgtttgcgcg gcaacatcct
     1021 gatgtcaatt tcaaacgacg aagggcgctt gttactctca acaggtaata aatcggaact
     1081 ggcactcggc tattgcacgc tttatggcga taccaacgga ggcttggcgg tgattggtga
     1141 cgtgctgaag acggaagtct atgccctcgc ccgtcacttc aattctgccc gtgagatcat
     1201 tccgctatcc atcatcaaca agcggccatc ggcggagctt gctccggatc agtttgacga
     1261 tcaatcgctg ccaccgtatg accagctcga tcccattttg cggatgtact ttgaggacaa
     1321 ggcgactccc gatgagatcg ttgcggcggg gaatagttca gagcttgtct atgacattct
     1381 taatcgggtg gaatctccgg cgaatgaatt taagcgcagg cagttgccgc ccacgctgat
     1441 tatttctcgc aatgcgattg gaattggacg gcgtcgtccg gtgacgcatc attacaagcg
     1501 ccggcccgcg gagaaaatgc agtcggcgga ctgagtggtt tgcaaagaaa ccgccaagtc
     1561 gccaaggaga aaacgagctt tgaacttggg actttgaatt ttgttcgctg atgaatttga
     1621 gcggcagatg taaaggctga gcttctgaaa caaagtacta agcacaaagt actaagcaca
     1681 aagttcaaag ctcgttttca cttggcagtt ttctttactt gccaatgacg aacgactcaa
     1741 gcaatatcct gctgtttcca acatccctcg gatcgaaaaa aactacaggt gagaaaaacc
     1801 atgcagaggt tcagctattc gtcctactta gtcgcagcag ttatggtgac gatcgtcgta
     1861 tcgtcggtgg tttcagctat tgcccagcag ccgtcagctc aaccaaaagc tcccgaggct
     1921 gaggtgaaag cggctgcggc catcagcagc gcgccagatt tggctgccaa gctgacgcag
     1981 gcggaagcct tcgctaaaaa gtatcccaag agcacgctgc gcccgcagat ggtgcaaggt
     2041 ctcactgcgg aaatcagccg agtaactgat gccgggcagc gtctggccct cgctgagcgg
     2101 tttctgaaag cgttttctaa cgacgtcgaa tcgggctaca tgaaagccct tgtcgttgat
     2161 gattacgtgc gggccaaccg cgttgatgag gcgttcagcc tgggcgctac gactttggca
     2221 aagcagccgg agaacctggg agtgctttcc gccctggcgc tagcgggaac tgaagaggcc
     2281 agaaagcaga atacgaaata tgcaacgcag gcctcgcagt atggagtgaa ggcgatcgaa
     2341 ttgatcgaga ccaataaaaa gccggcagat atggacgact ctttctggaa ctaccagaag
     2401 ttattactgc caaaactcta tctcagcatg ggggcgctgg cgatggctgc cggcaaaccg
     2461 gcggaagcca aaccgcgatt agaaaaggca attcaagtga atcccaccga accaactgcc
     2521 tacgtttttc tcggcgg
//
LOCUS       DASUEX010000542         2295 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_655784, whole genome shotgun sequence.
ACCESSION   DASUEX010000542 DASUEX010000000
VERSION     DASUEX010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_655784"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..785
                     /locus_tag="VFV61_09085"
     CDS             <1..785
                     /locus_tag="VFV61_09085"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX4900674.1"
                     /translation="VVFARGVPVFIDTEETGFLLNAEQIKRALTPRTKLIILNSPCNP
                     SGRVIAPAEFRKIMELLSSRGIYVISDECYLRFVYPPAEIFSAASLPANLRSRLCIAG
                     SFSKTYAMTGWRLGYALAPREWTKAMLNVQGHSTSNPNSIAQAAAVEALEGPQESVAL
                     MLAEYTARRAWLLRALKEIPNLKCDEPEGAFYAFPNVTGCYGSNVRNSEEFARQLLEQ
                     EQTVVTDGAGFGCEGYVRISYATSMSQLEEGVKRIRRFVEGL"
     gene            complement(865..>2295)
                     /locus_tag="VFV61_09090"
     CDS             complement(865..>2295)
                     /locus_tag="VFV61_09090"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEX4900675.1"
                     /translation="AIAPGPLPLGQKEVVKMKLCSTCQEEFADKFSFCPVDGTPLAAV
                     AEKIEESVTRPAVQQAPALMAEEVEESGTLPAAYSMLGLFSRKGASDARGAVSAASGN
                     GAGVFVPKSEYHLTFIDDAGLTQRLIGELKAVAHESELTWPEFKRDPFGFTKRMVVGY
                     GQAGWRVLSQPNVATGVLSALVVVIAMVLGVFLIDRIQTRSRAEQIAQNNEQLVDQIF
                     DIPAEQPTPDPGTAGLAKGSGGGSKPKPEKAAGGGGGGREEQKPASFGKTPQASLSIP
                     QVVAPDPHPPTIKNPSLPVAATVVADPLLVPPDARVLPYGDFKSKSNDPSSGPGSGNG
                     IGTGTGGGIGPGEGGGIGPGRGGNIGGGDRNDGGGGPGGPGGGDYNRIFSGKEVNSKA
                     RVLSKPEPQYTEEARKNQITGTVVLRAVFTSGGQVTSIRAVSGLPYGLTERAIAAARQ
                     IRFTPATKDGRPVSMYIQLEYNFNLY"
BASE COUNT          469 a          690 c          632 g          504 t
ORIGIN      
        1 tcgttgtttt tgcccgtggc gttcccgtct tcatcgacac cgaggagact gggtttctgc
       61 taaatgccga gcagattaaa cgcgccctca caccccggac aaaactgatc attctgaact
      121 cgccctgcaa cccatcagga cgcgtgattg ctccggccga gttccggaag atcatggagc
      181 tgctgagtag tcgcgggatc tacgtgattt cggacgagtg ttacctgcgc ttcgtctatc
      241 cgccggcgga aattttcagc gccgcgagtc tgcctgcgaa tcttcgcagc cgcctttgca
      301 tagccggttc cttctcaaaa acctatgcga tgaccggctg gcgtttaggg tatgccctgg
      361 caccgcgcga gtggaccaag gcgatgctaa atgttcaggg acactcgaca agcaatccga
      421 actcaatcgc tcaggcagca gccgtcgagg cgctcgaagg cccacaggaa tctgtggcgc
      481 tcatgctggc cgaatatacg gcgcgccgcg cgtggctcct gcgggcgttg aaggagatcc
      541 ccaacctcaa gtgcgacgag ccagagggcg cgttttacgc ttttcccaac gtgacgggtt
      601 gttacggcag taatgtgcga aactccgaag aattcgcgcg gcaactgctt gagcaggaac
      661 agactgtggt gacggatgga gctgggtttg gctgcgaggg ttatgtccga atctcctacg
      721 cgacttcgat gagtcaactg gaggaaggag tgaagcgaat cagacgcttc gttgaagggc
      781 tgtagcgcaa actgagagtt tgctacaaga caaaggcgct gagtggaatt ccagtcagcg
      841 cctttgtcat ttgggttttg cttattagta aaggttgaag ttgtattcca actggatgta
      901 catcgacacc ggccgaccat ccttagttgc cggcgtaaac ctgatttgac gcgccgccgc
      961 aatcgcacgc tccgtcaacc cgtaaggcaa acccgatacg gcgcggatgc tcgttacctg
     1021 accacctgaa gtaaacaccg cacgcagcac tactgttcca gtaatctggt tcttgcgcgc
     1081 ctcttcggtg tactgcggct ctggtttcga aaggacacga gccttggagt taacttcttt
     1141 tccgctgaag atgcgattgt agtcgccgcc gccggggccg ccagggccgc cgccaccgtc
     1201 attgcgatcg ccaccgccga tgttgccacc gcgacctggc cctatgccac cgccttcacc
     1261 ggggccgatt ccgccacctg tgccggtgcc gattccgtta ccgctgccag ggcctgacga
     1321 cggatcgtta ctctttgatt tgaaatctcc gtaaggaaga actcgcgcat ctggtggaac
     1381 cagcagcgga tcagctacca cggtcgcagc caccggcaat gaggggttct tgatcgtcgg
     1441 cggatgtgga tcgggagcca ctacttgcgg tatcgataaa gatgcttgcg gagtcttacc
     1501 aaaagatgct ggcttctgtt cctcgcgtcc gccgccgccg ccgccagcgg ccttctctgg
     1561 tttcggcttg ctaccgccgc cgctgccttt ggccaaaccg gcggtgccgg gatccggagt
     1621 cggctgttca gcgggaatat cgaaaatctg atcaacgagc tgttcgttgt tttgggcaat
     1681 ctgttcggcc cgactccgcg tctgaatccg gtcaatcaaa aagacaccaa gcaccatcgc
     1741 gatcacgacc acaagcgcgc tgaggacacc agtcgcaaca ttcggctgcg acaagactct
     1801 ccagccagcc tgcccatagc ccaccaccat gcgcttggta aagccaaaag ggtctctttt
     1861 gaattccggc caggttagtt ccgattcgtg ggcaactgct ttcagctctc caatcaatcg
     1921 ctgagtcagc ccagcgtcat caatgaaagt cagatgatat tcgctcttgg gcacgaagac
     1981 tccagcgcca tttcctgaag ctgcgctgac cgctccgcgc gcgtcagagg cacctttcct
     2041 ggagaaaagg cctagcatcg aataggcagc cggaagcgtc ccactttcct ctacctcttc
     2101 tgccatcaaa gctggcgctt gttgcaccgc cgggcgggtc acactttcct caatcttttc
     2161 agcaaccgcc gcgagcggcg tcccgtccac cggacaaaag ctgaatttgt ccgcaaactc
     2221 ttcctggcac gtggaacaaa gcttcatctt gactacctct ttctgcccta gcggaagcgg
     2281 tcccggggct atggc
//
LOCUS       DASUEX010000543         2069 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1597468, whole genome shotgun sequence.
ACCESSION   DASUEX010000543 DASUEX010000000
VERSION     DASUEX010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1597468"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="VFV61_09095"
     CDS             <1..319
                     /locus_tag="VFV61_09095"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX4900676.1"
                     /translation="ITGSRSIAGPVHYAASKGALEGLTRSLAHEVGPYGVLVNAIAAG
                     IFEGGLKTTIPEHHQKRYLDACALSRFGQPDECGEVVAWLGSRRNTYVNGTVIFLDGG
                     TLA"
     gene            430..1980
                     /locus_tag="VFV61_09100"
     CDS             430..1980
                     /locus_tag="VFV61_09100"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX4900677.1"
                     /translation="MIQLDSLFAGPPPKRDRDAAFFSYTRSICPTCKKLIDAHIVLRD
                     DKVWMQKKCPRHGFFEVEVSSDAEYYMKSLSYTKPGTVPYRFATKVEHGCPEDCGLCE
                     DHLQHTCSPILEINDVCDLTCPVCIVRNQQRYAMTFDEFKSAVDLLVAAEGELPIMLL
                     SGGEPTLHPQFFEFCDYVLRGPHEGKIKRLLISTHGRKIAQNRAFAEKFKAGGMYASL
                     QFDTLKAGVYPKTRGVELIDMKMQCIEVIKELDIPTVIVPTVSKGDNSDELGALVEFG
                     LELEQCSSIVIQPMAHTGDGGDGYHANPSGHRLTMPEVHQLIEEQTGWFKREYFHPVP
                     CSHPSCYTATYLFRMDDGKYKPLPEFVNIRQYLDAMQNRAIIRSDNQLEKMILDSITN
                     LWSATKVGEDSELILASLKSFLKDTFNSRNPLSAADIERRTEARSKALFIHNFMDPWD
                     LDVARLKKCCTHYVMPDGRLMPGCSYNNLYRHKDERFFPDAEPVSPAPVKIENGPALV
                     QITGRVSQ"
BASE COUNT          473 a          574 c          573 g          449 t
ORIGIN      
        1 gatcactggc tcacgctcga tcgccggacc cgttcattac gcggccagca aaggcgcgct
       61 tgaaggactc acacgcagcc ttgcccacga ggtggggcct tacggtgtac tcgtgaacgc
      121 gatcgccgcg ggaattttcg aagggggatt gaaaacgaca attcccgagc atcatcagaa
      181 gcgttatctg gacgcctgcg cgttgtcgcg ctttgggcaa cccgatgaat gtggcgaagt
      241 ggtcgcgtgg ctgggatcgc gtcgcaatac ttacgttaat ggcacggtca tatttcttga
      301 tggtggtacg ctggcttaga taaatccttc taatccgtgg ttaaaaacct actcaaacct
      361 ttcaaaatga actagaattt cgcgcgtcct cgtcgctgat atcaagcctt tccaaaagga
      421 gtgtgaatcg tgatccaact cgactcgctc ttcgccggtc ctcctccaaa gagggatcgc
      481 gatgccgcgt tcttttctta cactcgatcg atctgcccca cctgcaagaa actgattgac
      541 gcacacatcg tcctgcgcga cgacaaagtg tggatgcaga agaaatgccc gcgacacggt
      601 ttcttcgaag tcgaggtctc gagcgacgcc gagtactaca tgaagtcact ctcctacacg
      661 aagcccggca cagtgcctta tcgcttcgcc accaaagtcg agcacggctg tccggaagat
      721 tgcggcttgt gtgaagatca cctgcaacac acctgttcac caatcctcga gatcaacgac
      781 gtctgcgatc tgacatgtcc tgtttgcatc gttcgcaacc agcagcgcta cgcgatgacg
      841 tttgatgagt tcaaaagcgc ggtcgacttg ctggtcgcag cggaaggtga gctgccgatc
      901 atgctgctct ccggcggtga gccgacattg catccgcagt tcttcgagtt ttgcgactac
      961 gtcctgcgtg gtccacacga aggcaagatc aaacgactgc tgatctctac gcacgggcga
     1021 aagattgcac agaaccgcgc cttcgctgag aagttcaaag caggaggcat gtatgcgtcg
     1081 ctccagtttg acacgttgaa ggcgggtgtt tatccgaaga cgcgcggggt cgagctgatc
     1141 gacatgaaga tgcagtgcat cgaggtgatc aaggagctcg atattccgac cgtcattgtt
     1201 ccgacagtgt cgaaaggcga caacagcgac gagctcggcg cgctggtaga gtttggatta
     1261 gagctggagc agtgttcgag catcgtgatt cagccgatgg cccatacggg cgacggcggc
     1321 gacggttatc acgcgaatcc ttcaggccat agactcacga tgcccgaagt tcatcaactc
     1381 atcgaagagc agaccggctg gttcaagcgc gagtactttc atccggtgcc ttgctcgcat
     1441 ccgagttgtt acacggccac gtacctgttt cgcatggacg atggcaagta caagccgctg
     1501 ccggagtttg taaacatcag gcaatacctc gacgcgatgc aaaaccgcgc gatcattcgt
     1561 tccgataatc aactcgagaa gatgatcctc gattcgatca cgaacctctg gtcagcgacg
     1621 aaggttgggg aagattctga gttgatcctg gcgtcgctga agtcgttcct caaagacact
     1681 ttcaattcgc gcaatccgct ttcggcggca gacatcgagc ggcgcacaga ggcgcgcagc
     1741 aaggcgctct ttattcacaa cttcatggat ccgtgggacc tcgatgtcgc gcgcttgaag
     1801 aagtgctgca cgcattacgt gatgcccgac gggcgactca tgcccggctg ttcatataac
     1861 aacctgtatc gtcataagga cgaacgtttc ttccccgacg ctgagccggt atcgcctgcg
     1921 ccagtcaaga ttgaaaacgg accggcactg gtccagatca ccgggagagt cagtcaatag
     1981 caacgcccga gacaatcgcc gagctgcgag aattgatcat tcgcgcggcg ccggacgtga
     2041 atgcagcgat gcctgtgcgt gcttgctcg
//
LOCUS       DASUEX010000544         3726 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1143059, whole genome shotgun sequence.
ACCESSION   DASUEX010000544 DASUEX010000000
VERSION     DASUEX010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3726
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1143059"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1316)
                     /locus_tag="VFV61_09105"
     CDS             complement(<1..1316)
                     /locus_tag="VFV61_09105"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HEX4900678.1"
                     /translation="MSRHVILLTDAHEAPAYELIEALRAAGVKTLIEGLREVEAELAL
                     ARRQEREGVVEQLGPPPLAVLYEVVPGADPVELQTAIDHAKAFWPGAPLIACRRQVQG
                     YHRMELRHLDGATLKRLGFRAIADKAAQLPGLLREIEGHGATGELKLPEIIQRPVDTQ
                     SLSLPANLKAKSLRAAFDLVSSLHFIGDQIGAAQIALAGLEPLVDADRWSIYLLTDGK
                     GFESFGLEVIAVRVRGTEHDPNGRTWRDALLHEDKEQQPTKSTQLAAAGMGVNRKKEK
                     GIYVVAIPLICGDRILGVLEGTREHRGFKRADIELLDALSLPLASALANAVRIAEAER
                     LSQTDDLTKLHNARYMRQFLLNEIRRARRYGSNVAALFLDLDDFKKVNDRHGHLVGSH
                     VLMEVAAVILLSVRDTDAVARYGGDEFVVVLPDTDTELAGVVAERIR"
     gene            complement(1333..2583)
                     /locus_tag="VFV61_09110"
     CDS             complement(1333..2583)
                     /locus_tag="VFV61_09110"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HEX4900679.1"
                     /translation="MATRTRRYMFDWWVVQKRTIYLGIAVLIVLLLSGGAALYVWKFG
                     NPLKNVGTQVNNSAGARFISFEGDVRVIRAATRQTISASSDTELYPGDTVQTQATGRA
                     KIGMADGSTLLVRPNSIIIVRDNASAQDGKKTNVHVVVDSGQMFVRTEQLPEGTNNVV
                     ETPKTKNKLGEQTGASFGVNPEGTEEIRVNLGAVETTNQAGEKTNLRGGEYVSVNPSG
                     TVSRPQKLLDVPLPSEPRDLDKVFVGGNGSANIALRWLRPQSGSPAFYRVEVATSPFF
                     VADGKVIERDQLAATEFNASDLRPGVYFWRVRATAQSGQTSDWSDPKKFVVVAKGAAS
                     QVPVSNLSSELLGGNIYLIRGRAEPGTTIRAGGRESLVSTDRRFQIQITASPGTSEVT
                     VEAQDVQGNSSSYSVPLSSRSSRD"
     gene            complement(2878..>3726)
                     /locus_tag="VFV61_09115"
     CDS             complement(2878..>3726)
                     /locus_tag="VFV61_09115"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HEX4900680.1"
                     /translation="FKTPRIGFHAPIKGGLHEALIVSHELNCDTVQIFSRNPRGWKAK
                     LLTDDEIKLFKKVRRRTKISPLVIHANYLINLAAADAIMLQKSRDSFREELERGLILG
                     ADYLVVHPGSARGACEADGIRTCSESLKIASRGVNRGKLRILLENTAGQGECIGHRFE
                     HLREIMDLCPELSLGVCFDTAHAFTAGYDLREHDGLEALIKKIQKTVKTRNVRAIHFN
                     DSRAEYNSRVDRHWHIGEGHIGTEALRRVAQHPALAHAVFILETPYVDPRVDVQNLNK
                     LRSFVA"
BASE COUNT          789 a         1045 c         1055 g          837 t
ORIGIN      
        1 cgaatgcgct cggcgaccac acccgccagt tcggtgtccg tatcgggcaa gacaacaacg
       61 aactcatcac cgccgtaacg ggccacggca tcagtgtcgc gcacggacaa aagaatcacc
      121 gccgcgacct ccatcagcac atgcgagccg acgaggtggc cgtgtcgatc attgaccttc
      181 ttgaagtcgt cgaggtccag aaagagagcg gcgacgttgg agccataacg gcgtgcgcgt
      241 ctgatttcgt tcaagagaaa ctggcgcatg taccgcgcgt tatggagctt tgttagatcg
      301 tctgtttgcg agagtcgctc tgcttcggca atccgcacgg cgttggcgag tgccgaggcg
      361 agcggtaacg aaagtgcatc gagcagttcg atatcagctc gcttgaaccc tcggtgctca
      421 cgcgttcctt caagcactcc cagaattctg tcgccgcaga tcagcggaat tgctacgacg
      481 tagattccct tttccttttt tctgttcacg cccattccgg cagcggccag ttgcgtcgac
      541 tttgtcggct gttgttcttt gtcttcatgc agcaatgcat cgcgccaggt gcgaccgttt
      601 ggatcgtgct ctgttccccg tactcgcacc gcgatcactt ccaggccgaa cgactcaaaa
      661 cctttgccgt cagtcaggag atagatgctc cagcgatcgg cgtccacgag tggctccaat
      721 ccggcaagcg caatttgcgc ggcgccaatc tggtcgccga tgaagtgcaa agaagaaacg
      781 aggtcgaatg cggcgcgcaa actctttgct ttcaggtttg cgggcaacga aagcgactgt
      841 gtgtcgactg gacgttgaat gatttccggt agttttagtt cgccggtggc gccgtggccc
      901 tcgatttcgc gcagcaaacc agggagttga gccgccttgt cggcaatagc acgaaacccc
      961 agtcgcttta gagttgcgcc atcaagatga cgcagttcca tgcggtgata accctggact
     1021 tgccggcggc aggcaattag cggagctccg ggccaaaagg ctttcgcgtg atctatcgcg
     1081 gtttgcagct ctaccggatc tgctcccggt accacctcgt aaaggacagc cagcgggggc
     1141 gggcccaact gctcaaccac accctcacgc tcctgccgtc gtgccaacgc gagttctgcc
     1201 tcaacttctc gcaggccttc gatcaaagtt ttcactcctg cagcgcggag cgcttcaatc
     1261 agctcatacg cgggcgcttc gtgtgcgtca gtgagaagaa ttacgtgcct ggacatggat
     1321 tgattgctgg aattaatcgc gactgctgcg gctcgaaagg ggaacgctgt agctgctgct
     1381 gttgccctgt acatcctggg cttcgactgt cacctcggac gtgcctggtg acgcggtgat
     1441 ctgaatctga aagcgccggt cggtcgaaac caaactctcg cgaccgccag cgcgaatagt
     1501 tgtgcccggc tcagctcgac cgcgaatcaa gtagatgttg cccccgagaa gttcggatga
     1561 caggttggag acgggaactt gcgacgccgc acctttagcg acgacgacaa atttcttggg
     1621 gtcactccaa tcgctggtct gaccagattg ggccgtcgcc cgcacccgcc aaaagtagac
     1681 tccggggcga aggtcgctgg cgttgaactc ggtcgcggcc aattgatcgc gttcgatcac
     1741 tttgccgtcg gcaacaaaga agggggacgt tgcgacttca actcgataaa aggcaggcga
     1801 ccctgactgc ggtcttaacc atcgcagcgc gatgttggca gaaccgttgc cgccaacaaa
     1861 gactttgtcg aggtcgcgtg gttcagaggg caggggcaca tccagaagtt tttgtggccg
     1921 ggaaacagtt ccggaaggat ttactgaaac atattcacca cctctcaaat tcgttttttc
     1981 cccagcctgg tttgtagttt ctaccgcacc aaggttcacc ctgatttctt ccgtgccctc
     2041 cggattgact ccgaaacttg ctcccgtctg ttccccgagt ttgttcttgg tcttcggggt
     2101 ctcaactacg ttgttggttc cttcgggaag ttgctcagtg cgaacaaaca tctgaccgct
     2161 atctacaaca acgtgcacgt tagtcttctt cccatcctga gcactggcgt tgtcgcgcac
     2221 gatgatgatg ctgttgggcc gaaccaggag tgtcgatccg tcggccatcc caatcttcgc
     2281 gcgtccagtg gcctgagttt gtaccgtgtc gccgggatag agttcagtgt cgctgctggc
     2341 gctgatagtt tgccgtgtag cagcccgaat cacgcgcaca tcgccttcga aggaaatgaa
     2401 tctggctccg gcggaattat tgacttgtgt gccgacgttc ttgagcgggt tgccaaactt
     2461 ccaaacgtac agcgcggcgc caccgctaag taacaacacg atgagcacag ctatgcccag
     2521 gtagatcgtg cgtttctgca cgacccacca atcaaacatg taacgccgcg ttctggtggc
     2581 catgattacc gagaataaaa ctgcggggca atttagctgt gagtgatccg agaaaggtcg
     2641 ggttggaacg atcaggcttc cgggatcaaa aggtgtggag cagcggaagc agcgggcccg
     2701 attcaaggtt taaccccaac ccactgctga acaaaaattt aacacacgcg cgagacgcgt
     2761 tacaagcacg ttgtttttag cagctgaatc gtggagcgtc ccttcgtggg cgcccgctcg
     2821 cgggtaggga gccccctgca aacaggaccc ggcggttttt aggttcattt gcgggcttta
     2881 agccacgaaa gagcgcagct tgttgagatt ctggacatcc actctcgggt cgacataagg
     2941 ggtttcgaga ataaaaaccg cgtgggcgag tgcgggatgc tgagcgaccc ggcgcagagc
     3001 ctcagttccg atgtggccct cgccgatgtg ccagtgccga tcaacgcgcg aattgtactc
     3061 agcgcgcgag tcgttgaaat gaatggcacg aacattcctc gtcttgacgg ttttctgaat
     3121 cttcttgatc aaggcttcga gcccgtcgtg ttcgcgcagg tcatacccag cggtgaatgc
     3181 gtgagcagta tcgaaacaaa caccaagtga cagttcgggg cagagatcca taatctcgcg
     3241 caagtgctca aagcgatgac cgatacactc gccctgaccg gcagtattct ccaggagtat
     3301 tcgtagcttc ccccgattca cgccgcgcga agcgattttc aaactctcgc tgcaagtacg
     3361 aatgccgtcg gcttcacacg cccctcgcgc gctgccggga tggactacaa ggtagtcggc
     3421 accgagtatt agcccacgtt ccagctcctc gcgaaacgag tcgcgcgact tttgcagcat
     3481 gattgcatcg gcagcagcaa gattgatcag gtagtttgcg tgaattacca gaggtgagat
     3541 cttggtgcgc cgacgaactt ttttgaagag tttgatttcg tcgtcagtga gaagcttggc
     3601 cttccagccg cgcggattgc gcgagaagat ctgtaccgtg tcgcagttga gttcgtgcga
     3661 aacaatgagt gcctcatgca atccaccctt gatcggtgcg tggaagccaa tgcgaggggt
     3721 tttaaa
//
LOCUS       DASUEX010000545         3108 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_655791, whole genome shotgun sequence.
ACCESSION   DASUEX010000545 DASUEX010000000
VERSION     DASUEX010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3108
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_655791"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="VFV61_09120"
     CDS             complement(<1..561)
                     /locus_tag="VFV61_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900681.1"
                     /translation="MQQARTLGELKSQGYKSVSVKDELRHNLVRKLQRGEKLFPGIVG
                     YDETVIPQLVNAILARHNVILLGLRGQAKSRIIRQLTELLDEHIPIIAGSEVNDDPLN
                     PISAYGRQTVDLHGEQTHIDWIGRDARFVEKLATPDVTIADIIGDVDPIKAAKGGHLL
                     SDELTIHYGLLPRANRGIFAINELPDL"
     gene            complement(608..2110)
                     /locus_tag="VFV61_09125"
     CDS             complement(608..2110)
                     /locus_tag="VFV61_09125"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX4900682.1"
                     /translation="MQSPKVTTPRDAAAVILLRHNTNPTEPEVYWVRRSEKLAFLGGF
                     HAFPGGQLDQPDTLIPVDNSPDAETAAAISCAARELFEELGVLLARGGDLLTRGQRAS
                     LLDDLQSGRMTWPDLLKHYDLHLDARDYSFAGRWVTPPFSARRFDTWFFLACCPPRQE
                     PQIIPGELDFGEWISARSAFQRWIQGKVVAVPPTLHALKTLSGGLTPDLTERFLSIPQ
                     AHRGLVRSIEFRPNYICVPVRTPTKPPATHTNAYLVYTTREVLVIDPASPYAEEQRSL
                     AECVDELTRDGRRVRGVLLTHLHPDHVGGVNALREHLGGAAVCAHRLTAEALAGSITI
                     DRIIEDEDLVQLDGEPSITLRALHTPGHARGHLCLYEESTGTLISGDNIVGLGSVLID
                     PPEGNMQQYLDSLRRMRSLQNLSIILGGHGPAIANPQAKLDEYINHRLEREAAILAAV
                     RNGAATPQEIVRVVYTDVSPKAYSMAERAVLAHLQKLSEEGMVGGVKIDD"
     gene            complement(2176..2859)
                     /locus_tag="VFV61_09130"
     CDS             complement(2176..2859)
                     /locus_tag="VFV61_09130"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase-like oxidoreductase"
                     /protein_id="HEX4900683.1"
                     /translation="MSFLGSLFNDPKYQEHDPRSPDEIADSEIIISPDTRRDDRMPPG
                     QSRTKKWPVLDAHGTPEIDLASWEFEVDGLVERPQKWSLDEFMQLPAVKVYADFHCVT
                     RWSRLDNVWGGVPTRELAKIAGLKPVAKFALALAYDYGWTTNIPIEYFLKEDSLLVWS
                     HDGQPIPPEHGGPVRLIIPQLYAWKSAKWIKGIRFLESDRAGYWEEGGYHMRGNPWNG
                     ADGERFRWS"
     gene            complement(2864..>3108)
                     /locus_tag="VFV61_09135"
     CDS             complement(2864..>3108)
                     /locus_tag="VFV61_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900684.1"
                     /translation="WSAGLSLVRRGIQFQDWKEIVNGILLSLEQTENYERQRGPEGDR
                     DNWHDRSIGIEESTSKAVGKWMPTELMDLAEKSLKK"
BASE COUNT          677 a          853 c          870 g          708 t
ORIGIN      
        1 cagatccggc agctcgttga tagcaaagat cccgcggttt gcgcggggca gcagaccata
       61 gtgaatcgtc agttcgtcgc taagcaggtg gccacccttc gccgctttga ttggatcgac
      121 gtcgccgatg atgtcggcaa tggttacgtc tggcgttgcc agcttttcca caaaccgagc
      181 atcgcgaccg atccaatcaa tgtgcgtctg ctcgccatgg agatcgaccg tctgtcggcc
      241 gtaggcagag atcggattca atgggtcgtc gttaacttcg gagccggcga tgatgggaat
      301 gtgttcgtcg aggagttctg tcaactggcg gatgatccgg cttttggctt ggccccgaag
      361 gccgagaaga atcacattgt gccgggccag gatggcgttt accagttgag ggatgaccgt
      421 ttcatcgtag ccgacgatgc caggaaacag tttctcgcct cgctgaagtt tgcggacgag
      481 gttgtggcgc agttcgtctt ttaccgaaac gctcttgtag ccctgcgact tcagctcgcc
      541 gagtgttctt gcctgttgca ttgtcgaatt gtcctgaaat ttgtcgcggt ccgtaaactg
      601 agaatcgtca atcgtcgatc ttcactccac caaccattcc ttcttcacta agtttttgga
      661 gatgcgccag cacagcccgc tccgccatgc tgtaagcttt gggggagaca tcagtgtaca
      721 cgactcgaac gatttcttgc ggtgtcgcgg ctccatttcg gaccgcggcg agaattgccg
      781 cctccctttc cagccggtga ttgatgtatt cgtcgagttt cgcctgggga ttggcgatag
      841 cgggcccatg accgccaaga atgatcgaga gattttggag agaacgcatg cgccggagcg
      901 agtcgagata ctgctgcata tttccttccg gcggatcgat cagaacggag ccgagcccca
      961 caatgttatc gccggaaata agagtgccag tactctcttc gtagagacaa agatgaccac
     1021 gtgcatgacc cggcgtgtgt agcgcgcgca aagtgatcga aggctcaccg tcgagttgaa
     1081 ccagatcctc atcttcgatg attcgatcga tggtaatcga accggcgaga gcttcagcgg
     1141 ttaaccggtg cgcacagacg gcggcacctc caaggtgttc ccgcagagca ttcactcctc
     1201 cgacgtgatc tgggtgcaag tgggtcagca atactccgcg gacacgtcga ccgtctcgag
     1261 tgagttcgtc aacgcactct gccaatgaac gctgttcttc agcgtagggt gaagccgggt
     1321 caataacgag gacttctctg gtggtgtaaa ccaggtacgc attcgtatga gtggccggcg
     1381 gtttggttgg agtgcgaacc ggaacacaaa tgtagttggg tcgaaactcg atgctccgaa
     1441 ccaagccacg atgtgcttgt ggaatggaaa ggaatcgttc cgttaaatct ggtgtgagtc
     1501 ccccggataa ggttttgaga gcatgaaggg tcggaggcac tgcaacaacc ttcccctgga
     1561 tccatctctg aaaagcactg cgcgcggaaa tccactcacc aaaatccaac tcaccaggaa
     1621 tgatttgcgg ctcttggcgt ggcggacaac aggctagaaa gaaccaggta tcaaaacgcc
     1681 tggcgctgaa aggcggggtc acccagcgac cggcaaagct gtagtcccgg gcatccagat
     1741 gaagatcgta atgtttcagc agatccggcc acgtcatccg cccagactgt agatcatcga
     1801 gtaaggacgc acgctggccc ctggtgagta ggtcaccacc gcgcgcgagc agtactccga
     1861 gctcttcaaa cagttcacgc gcagcacaac ttatcgcggc tgcagtttca gcgtccgggc
     1921 tgttatcaac gggaataaga gtgtctggct ggtcgagttg accaccgggg aaagcgtgaa
     1981 aacctccgag gaaggcgagt ttctcacttc gtcgaaccca gtagacctcc ggctcggttg
     2041 gattcgtgtt gtggcggaga agtatgacgg cggcggcgtc tcggggcgta gtgactttag
     2101 gagactgcat cgcttactag aaccaaaccg agcttggacg ttggacgttt gatccgactc
     2161 tcaacaaggc ccggatcatg accatcgaaa gcgctcgccg tctgcaccgt tccacggatt
     2221 gccgcgcatg tggtaaccac cttcttccca atacccagca cgatccgact cgagaaaacg
     2281 aatacccttg atccatttcg ccgacttcca ggcgtagagc tgtggaatga tcagacgaac
     2341 tggtccaccg tgctcaggcg ggatgggttg tccatcatgt gaccagacca aaagtgagtc
     2401 ttccttgagg aaatattcga tcggaatgtt cgtggtccat ccataatcgt aggccagcgc
     2461 cagggcgaat tttgccacag gcttaaggcc agcgatcttt gccagctcac gggtcggaac
     2521 gccaccccag acgttgtcca gacgtgacca gcgcgtgacg cagtgaaagt ctgcatagac
     2581 cttaaccgcc ggcagttgca taaactcatc gagcgaccac ttctgcggac gttctaccag
     2641 gccgtcgacc tcaaactccc aactggccag atcaatttcc ggagttccat gagcatcgag
     2701 caccggccat ttctttgttc gtgactggcc tggaggcatg cggtcgtcgc ggcgggtatc
     2761 gggactgatg atgatctcgg agtccgcgat ctcgtcaggg gaacgcggat cgtgctcctg
     2821 gtatttcggg tcgttgaata ggctgccaag gaaactcata tcgttacttt ttcagactct
     2881 tttctgcgag gtccatcaac tctgtcggca tccatttgcc cactgcctta ctggttgatt
     2941 cctcgattcc gatgctgcgg tcatgccaat tatcccggtc accttccggt ccgcgctgtc
     3001 gttcgtagtt ctcagtttgc tcaaggctaa gcaggatccc gtttacaatc tctttccaat
     3061 cctgaaactg aatcccgcgt ctgaccaaag agagtcccgc cgaccaat
//
LOCUS       DASUEX010000546         4799 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_432134, whole genome shotgun sequence.
ACCESSION   DASUEX010000546 DASUEX010000000
VERSION     DASUEX010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4799
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432134"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..658)
                     /locus_tag="VFV61_09140"
     CDS             complement(<1..658)
                     /locus_tag="VFV61_09140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900685.1"
                     /translation="MNSSAHGARWVLVATVSLLLLVFVWDANVTAQSGRRKDTKPKAR
                     PAPSPIAEANRPASSQTPVSISSPSPTTPGEDVEESDIVRVSSNLVPIPASVVDNKGT
                     ALTTLKVDDFELRVDGQVRPISDISRSDSPVRLAMLFDNSGSLDMGRAFEKHAAQQFF
                     RRVLRPSDEAAIYSVESESYLAQPMTRDIRRLEQTIELFGKPEGSTSLFDTIIAAAAY
                     L"
     gene            complement(655..2007)
                     /gene="trmFO"
                     /locus_tag="VFV61_09145"
     CDS             complement(655..2007)
                     /gene="trmFO"
                     /locus_tag="VFV61_09145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEX4900686.1"
                     /translation="MNEVTIIGAGLAGVEAAWQAARAGAAVRLYEMRPLKQTPAHRTD
                     KLAEIVCSNSLKSDEPGSAPFLLKEELRRAGSVVMEAAAATRVPAGAALAVDRQLFAE
                     YITDRIEAHPRIQLVREEMTEIPADVITIIATGPLSSDALTAEIMKLTGSDQLYFYDA
                     IAPIITADSIDRTIAFAAARYGKGGDDYLNCPFDEAEYARFYNALIEAKSVPLQRFEE
                     TRWFEACLPIEELARRGVDTLRFGPMKPVGLADPRTGREPYAAVQLRQENLMADAYSF
                     VGFQNHLRYGEQARVLRLIPGLERAEFLQFGQIHRNTYICSPRVLSETMQMRERPNVF
                     FAGQITGVEGYVESVAMGWLAGQNAARLAGNHGLITAPGRSAIGALARYVTNAESKHF
                     QPVNITFALLQPLEEQDRRRLRKKRDRHQFQVELALTEWDAWLKDLQLELATMPARAS
                     "
     gene            complement(2004..3638)
                     /locus_tag="VFV61_09150"
     CDS             complement(2004..3638)
                     /locus_tag="VFV61_09150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900687.1"
                     /translation="MSAPILTHPSGLKLAERQRAITIFVAALLTVLLISACREIRAQS
                     DSPAKKLPSAEKVVENYLKALGGKKKLSAIRDASFEWTIQLNNQPIGKARTQIKSPAS
                     RRSEMKFGNGEIVSGATPASAWVLGLDGRLVTLTGAEAAVAKLQALLEASHLINYKKL
                     NVMARVISLGDLASDPAYIIEFSHRSGARLRYWFSVKSNLLVKVEDELRNTTMRFADY
                     QSTSQAPGAREPQRLTLKTGDGELTYKLERASYNSNLPSATFDPPRREETLDVVGLLR
                     AVAQNQDDVEKRVSEYAFLQKETDRELNDKGELKKESIRVYEVFPLANREPIRKLISE
                     NGVALSTERAAKETKRVTEEFEKAERERAKTDQRAARRRTERAQRANKGDQEEEDPEI
                     SQFLKVCEFVSPRREVFGGREAIVFDFRPRSGFKPSNRTETLISKLVGVAWIDPIDKQ
                     VMRLEARLAESFKMGGGLLLSLRPGAGLIMEQTRMAEGVWLPRLAQLNLSARVFLFRG
                     IDLNKTIEWSDYRHFQGDVNDYQLDAPKAEPPAQKP"
     gene            complement(3646..4098)
                     /locus_tag="VFV61_09155"
     CDS             complement(3646..4098)
                     /locus_tag="VFV61_09155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900688.1"
                     /translation="MGSRDQMRNLEVFKVGADAVNEFEYQKNQGELTEQREHHLERPD
                     SPSGPSTEAERIEQVIADAHAKVEERKRKAARGAAKKAAAKNSPGKRSTKKSAAKKSS
                     VKKSARRSVAKSTAKKSAAKRTPARKTASKKKSTARKSPARKAGAKRS"
     gene            4192..4267
                     /locus_tag="VFV61_09160"
     tRNA            4192..4267
                     /locus_tag="VFV61_09160"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4225..4227,aa:Thr,seq:cgt)
BASE COUNT         1023 a         1330 c         1248 g         1198 t
ORIGIN      
        1 gcaagtaggc ggccgcggca atgatggtat cgaacagcga ggttgagccc tcgggttttc
       61 cgaatagctc aatcgtttgc tccagtcttc ttatatctct cgtcatcggc tgcgcgagat
      121 aactttccga ttcaaccgag taaatggcgg cttcatcgct gggacgcaag acacgacgga
      181 aaaactgttg tgccgcgtgc ttctcgaacg cccgccccat atcaagactg ccactattgt
      241 cgaatagcat tgccaatctc actggtgaat ctgagcggct gatatcgctg attggtctga
      301 cctggccatc gactcgtaac tcaaaatcgt caaccttcaa agtggtgagc gcagttccct
      361 tgttatcgac gactgaggca gggatgggaa caagattgga agacacacga acgatgtcag
      421 actcttcgac atcctcaccg ggggttgtgg gggaaggcga ggatatagag accggagttt
      481 gagaactggc aggtcggttc gcttcagcta tcggacttgg cgctggtcgg gcttttggtt
      541 tggtgtcctt gcgccggcct gactgcgccg tcacgttggc atcccacacg aagacgagca
      601 gcagtagtga gaccgttgct actaaaaccc acctggcgcc gtgcgcagaa gagttcatga
      661 ggctctcgcc ggcatcgttg ccagttctag ctgcaaatct tttagccaag cgtcccattc
      721 tgttaacgcc aactccacct ggaattgatg acggtcacgt ttcttacgaa ggcggcgccg
      781 atcttgttct tctaacggct gtagcaaagc aaaagtgata ttaacaggct gaaagtgttt
      841 gctctcagca ttggtcacgt aacgtgcaag agcaccgatc gcgctgcggc ccggagcggt
      901 aatcaacccg tggttgccag caagccgcgc tgcgttttgt cccgccagcc accccatagc
      961 aactgactcg acatatcctt cgactccggt gatctgaccg gcaaagaaga cgtttggcct
     1021 ttctcgcatc tgcatggtct cgcttagcac gcgcggcgaa cagatgtagg tgttgcgatg
     1081 aatttggcca aactgtaaga actcggcgcg ctcaagtccg ggaattaagc gcaacactct
     1141 cgcttgctcg ccgtagcgta gatgattctg aaagcctaca aagctgtacg catcggccat
     1201 taaattttcc tgacgcaact gcaccgcagc gtacggctca cgaccggtgc gcggatcggc
     1261 aagtcccact ggcttcatgg gaccgaaacg caaagtatcg actcctcgtc gtgccaactc
     1321 ttcgattggc aggcacgctt caaaccagcg cgtctcttcg aaacgctgca gaggaacact
     1381 cttcgcttcg attaaagcgt tgtagaagcg ggcatactcg gcttcatcga acgggcagtt
     1441 cagatagtca tcgccacctt tgccatagcg ggcggcggcg aaagcaattg ttcgatcaat
     1501 cgagtcagca gtaataatcg gtgcgatcgc atcataaaaa tagagctggt cgctgccggt
     1561 gagtttcatg atctcggctg ttaaggcatc cgatgacaaa ggaccggtgg cgatgattgt
     1621 gatcacgtca gcagggattt ccgtcatttc ttcacgcacc aattgaattc gaggatgagc
     1681 ttcgattcga tcggtgatgt actcagcaaa aagctgacgg tcgacggcca gggcagcacc
     1741 ggcaggcacg cgtgtggcag cagccgcttc cataacgacg gaaccggccc gccgcaattc
     1801 ttctttcaat aagaaaggcg ccgatcccgg ctcatctgat ttgagtgaat tggaacaaac
     1861 tatttcggcg agtttgtcag tacggtgcgc gggcgtttgt ttcaagggcc gcatctcgta
     1921 gagtcttacg gccgctccgg cacgtgccgc ttgccatgcc gcctcgaccc cggcgagtcc
     1981 ggccccaatt attgttactt cattcacggt ttttgagcgg gcggttcagc ttttggggca
     2041 tcgagttggt aatcgtttac gtcaccctga aagtgccggt agtcgctcca ttcgatcgtt
     2101 ttgttcaaat caatcccgcg aaagaggaac acccgggccg acagatttag ttgagccagg
     2161 cggggcaacc aaactccttc ggccattcgc gtttgctcca tgattaaacc ggcgccggga
     2221 cgtaacgaca agagaagccc acctcccatt ttgaaacttt cggcgagacg agcctcgaga
     2281 cgcatcacct gtttatctat tggatctatc caggccacac ccacaagttt agagatcaac
     2341 gtctcggtgc gatttgacgg cttaaatccg gaacgtgggc gaaaatcaaa aacgatcgct
     2401 tcgcgtccgc caaacacttc gcgacgcgga gaaacgaact cgcatacttt cagaaactgc
     2461 gagatctccg ggtcctcttc ctcctgatct cctttgttcg ctcgctgcgc gcgttcggtg
     2521 cggcgtcggg cagcgcgctg atccgttttt gcgcgttcgc gctcagcctt ttcaaattcc
     2581 tccgtcactc gtttcgtttc tttcgcggct cgttcagttg acagagcgac tccgttctcg
     2641 ctaatcaatt tcctgatcgg ttcgcggtta gccaggggaa acacttcata aactctaatg
     2701 gattccttct taagctcgcc tttgtcgttt aattcgcgat cagtttcttt ttgcaggaac
     2761 gcgtattcgc tgactcgctt ttcgacatcg tcctggtttt gggccacggc ccgcagcaat
     2821 cccacgacgt ccaacgtttc ctcccttcgc ggaggatcga aagtcgcact cggcaggttt
     2881 gagttatatg aggctcgctc gagtttgtag gtcagttccc cgtccccggt cttgagagtc
     2941 aggcgctgtg gttcccgcgc ccctggtgct tgcgaagtgg attggtagtc ggcgaaccgc
     3001 attgtcgtat tgcgcaattc gtcttccact ttcaccagca ggttgctctt gacgctgaac
     3061 caatagcgca aacgggcgcc cgaacgatgg gaaaactcga tgatgtaagc gggatcggaa
     3121 gcgagatctc cgagcgagat tacgcgcgcc ataacattca atttcttgta attgattaaa
     3181 tggctggctt cgagtaacgc ctggagtttt gcgacagcgg cttcggcccc ggttaacgtc
     3241 accaggcgac cgtccaaacc caatacccac gcggacgcgg gagtggcgcc cgaaacaatc
     3301 tcaccgttgc caaacttcat ttccgatcgg cgcgacgccg gtgacttaat ctgggtcctg
     3361 gctttgccga tcggttggtt gttgagttgg atcgtccatt caaagctcgc atcgcggatc
     3421 gcgctgagct ttttcttgcc tcctaaagcc ttgaggtaat tctcaacaac cttctcggca
     3481 ctcggaagct tctttgccgg cgaatcactt tgcgcgcgaa tttcgcggca ggcactgatc
     3541 agcagaaccg taagcagcgc cgccacgaag atggtgatgg cgcgctgtcg ctctgctaac
     3601 ttcaaacctg acgggtgagt aagaatgggg gcactcatga ggttcttatg atcgctttgc
     3661 acctgccttc ctggctggag atttcctggc tgtagacttc ttttttgacg ctgtctttct
     3721 ggccggggtc ctcttagcgg cggacttctt cgctgtagat ttagctaccg accgccttgc
     3781 tgacttcttc actgaagact tcttcgccgc cgatttcttc gttgatcttt tgcccggcga
     3841 attcttagcg gctgcctttt tggcagcgcc acgcgcagcc ttccgtttcc gctcctcaac
     3901 cttcgcgtgc gcgtcagcaa ttacttgctc aatgcgttcg gcctccgtcg acggacccga
     3961 aggcgagtcc ggtcgctcca aatgatgttc gcgctgctcc gttagctcac cctgattctt
     4021 ctggtattca aactcattaa ctgcgtcagc accgactttg aatacttcga gattgcgcat
     4081 ctggtctctg cttcccatgt tcgttttcct ccttaagaat ggctgcttga caccccaatc
     4141 agcgggttcg tatagtgcaa ttcttcaacg cgacaagcgt ttcgcattag agccggctta
     4201 gctcagttgg tagagcgtct gattcgtaat tagaaggtcg ccggttcaat tccggcagcc
     4261 ggctccatgt tttcctgaaa atggatcata caggatccat ctccagtagc cagagaacgg
     4321 gcgcatgcac aatggctgcc cgcctgtgga aaagtcgttg actcgttcca ttaacaggta
     4381 tatataagcc gatcagaagg aataagagta cgtcgagtcg gtaaacctta cggagtttta
     4441 ctcccttaat tgttgttcct gggaggccag ttcctgactc agacttcagg aaaattcaaa
     4501 ccgcagtttt cacataccgt cggcctcgcg tcctattgga tccacagtct gcagattaga
     4561 tctcgagatt tttcggccag cgacattgcc gcgcacagct tgacggctca ggtaatgctg
     4621 aaattagcaa aacttgacta aaacctgcct aagagcagac gagattcggc attaccaacc
     4681 aaggtacgac cgaaccatat aggagaattt acgatgaacg cccttaaagt cactatcgct
     4741 tcttgttgcc tcgtgatggc gctttttcag tccagtctcg ggcaacaagt gtcgggacc
//
LOCUS       DASUEX010000547         4621 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_779264, whole genome shotgun sequence.
ACCESSION   DASUEX010000547 DASUEX010000000
VERSION     DASUEX010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4621
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_779264"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1135
                     /locus_tag="VFV61_09165"
     CDS             <1..1135
                     /locus_tag="VFV61_09165"
                     /inference="COORDINATES: protein motif:HMM:NF021492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX4900689.1"
                     /translation="QGKLKEAEGFFLRAIGVDNRLIGAHMNLAYLYLLRGEPDKTAAQ
                     LEKVLSLDPANADAAYRLAWVLFSQGKFDECIALLDKVKESQTLSAPTLAVLGDAYLR
                     KGDAVKAEENYLLALDVQSNNADALLGLAQLSAAKGDTTMAVAYVDRASNVTYSPELL
                     YKLARVGLNLNLKEQAVAALKQAVLLRPAEPSYHFALGTAWLKQPPDLQSAEQSFRQF
                     LKLKPDDVQGQLHLGYVLLKQKKQADARTWLLKSVGNGVGTPEAFYYLGLIAQAENGD
                     AEAIQLFQKSVQLAPSFASAHVALGSSYLKQKDYVRAQEALEAGVKLSPDDSKAHYNL
                     AMLYARLKNPEKAQEEMRIVERLKSQGKEPQDDSGSLAPPAPR"
     gene            1294..2958
                     /locus_tag="VFV61_09170"
     CDS             1294..2958
                     /locus_tag="VFV61_09170"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HEX4900690.1"
                     /translation="MLVTILILNLPMESRGQRPNEGVKSGITFVEVPAKASGIGWVHT
                     NAHSPERFLPETVGAGGAFFDYDNDGWMDIYLVNSGAADFYTPKSPIKNALFRNNRDG
                     TFTDVTDKAGVAGGRFGMGAAASDYDGDGWQDLFVTNYGADILYRNNGNGTFTDVTEK
                     AGVGVTGWSTCAVWFDYDGDGKLDLFVSSFIEFDRSQHRLCVDKTVTQNYYCIPRIFK
                     PRPSHLFRNNGNGTFTDMSRDSGISGSPGKSFGAVATDINKDGLMDLFVANDTMPNFL
                     FINKGNGKFEESGLAAGVSYGEAGTPRSGMGVDAEDYDGDGWQDLFVANIDQEFFSLY
                     HNQMDLTFTDQPGEIAPSTQLLSGWGLKFFDYDNDGDPDLLLANGHPDDMVETKSARV
                     KYKEPLLLFENTGKTYRNVSALSGAVFAKSFPARGMAVGDFDNDGDVDVLVSNNGEAP
                     LLLRNEGGNRNNWLGLHLVSTKSNPAAVGAVITWQAGGVRRSRLKTSGGSYLASHDPR
                     EVLGLRSAAIVESVEIRWPSGVVDKLSRLPVNTYLKVVEGRGVVKN"
     gene            complement(2968..>4621)
                     /locus_tag="VFV61_09175"
     CDS             complement(2968..>4621)
                     /locus_tag="VFV61_09175"
                     /inference="COORDINATES: protein motif:HMM:NF012931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HEX4900691.1"
                     /translation="IQDRPRFPWRGLLIDVARHFQPMDVLKRNLDAMAAVKLNVFHWH
                     LTEDQGFRIESKKFPRLHQLGSDGHFYTQDQAREIISYARDRGIRVVPEFDLPGHATS
                     WLVGHPELGSAPGPYTIERKPGIFEPALDPTREEVYKFLDTFFGEMAGLFPDAYLHIG
                     GDENEGKQWDRNPQIQAFMKSKGIKDNHALQTYFNKRTLQILKKHGKIMMGWDEIFQP
                     DLPQEVVIHSWRGPGALAEAARKGYKGVLSAGYYIDLIFPTSQHYAVDPIPADSTLTA
                     TEAARVLGGEATMWSEWVTPDTIDSRIWPRTAAIAERLWSPRSVTDVDDMYRRLAVTS
                     LRLEEVGLTHEKNQAMLLRRLAQGREIGPLQTLVSVVEPVKEYRRYQQRPQTMLSPLT
                     GLIDAARPDSDAARRFAATVDSLLSDAPRFEAHSQQVRTTLTQWRNAGPELEAMIDRS
                     PALHEVKPLAKDLNELGTIGLEALSYISRGITPTPEWRNARLTALDQAANPKGGLEFP
                     IIPGVRQLVIAASELHRLQHMTPAAWKAQVKALAAPAKQAAN"
BASE COUNT         1014 a         1259 c         1336 g         1012 t
ORIGIN      
        1 gcaaggaaag ctaaaggagg cagaaggttt tttcttacgc gctatcggcg tcgacaatcg
       61 tctgatcggg gcgcacatga acctggccta tctttatctg ctcagaggcg agccggataa
      121 gaccgcggcg cagctcgaaa aagttctgag tttggatccg gcgaacgcgg atgcggcgta
      181 cagactcgcg tgggtgttgt tttcccaggg gaagttcgac gagtgcattg ccctgctcga
      241 taaggtgaag gaatcgcaaa cgctctcagc tcctaccctg gcagtgcttg gtgatgcgta
      301 cctcaggaaa ggtgacgcgg taaaggcgga ggagaactat cttctggcgc tcgatgtaca
      361 gagcaacaat gctgacgcgc ttctcggcct cgcccagcta tcggcggcca aaggcgacac
      421 aacaatggcg gttgcctatg tcgatcgtgc aagtaatgtc acctactcgc ctgaactact
      481 ttacaagctg gctcgagttg gtctgaacct gaatttgaag gagcaagccg tggctgcatt
      541 gaagcaggcc gtccttctca ggcccgctga accgtcgtac cactttgccc tgggcaccgc
      601 atggctcaaa caaccacccg atctccagag cgccgaacaa tccttccggc aattcttaaa
      661 gttgaaaccc gacgatgtgc agggccaact gcaccttggc tacgtgctgc tcaaacagaa
      721 aaagcaggct gacgcacgaa cttggctctt gaagagtgtt ggcaacggcg tcgggacgcc
      781 ggaagctttt tattacctcg gtctgatcgc tcaggccgag aacggagacg ccgaagcgat
      841 acaactcttt cagaagtcag ttcaactggc gccttcgttt gccagtgccc acgtggcgtt
      901 gggctcaagc tacctgaagc agaaagatta tgtgcgcgcc caagaggcgc tagaggctgg
      961 ggtgaagttg agtcccgacg attcaaaagc gcactacaat ctcgccatgc tttacgcccg
     1021 tcttaaaaat ccggagaagg cgcaggaaga gatgcgcata gttgagagac taaagagcca
     1081 gggcaaggaa ccacaggatg atagcggctc gctcgcacca ccagccccgc gctagcctcc
     1141 tatgcttaag cgtcggccgt tatcacttgt atgaacctcc aggaagccac cagagatcac
     1201 acgaccagac caagttgttt cgcgcgactt cgtctggttt cctgtatccc ttcctcctct
     1261 ttatcggcaa tgcggtttgc gattctcacc gcaatgttag tcaccatttt gattcttaac
     1321 ctgccgatgg aatcccgcgg tcaacgtccg aatgagggcg tcaaatcagg aatcactttc
     1381 gtcgaggtgc cggctaaggc aagcgggatc ggttgggtcc atactaacgc gcactcacca
     1441 gaacgcttcc tgcctgagac ggttggggcg ggcggcgctt tcttcgacta cgacaatgac
     1501 gggtggatgg acatctacct ggtcaatagc ggcgcagcgg atttctacac gcctaagagt
     1561 ccgatcaaga atgcgctctt tcggaataac cgcgacggca ctttcactga tgtcaccgat
     1621 aaagccggtg tggctggcgg taggttcgga atgggcgcag cggcaagtga ttacgacggc
     1681 gatggttggc aggacctttt cgtcactaac tatggagctg acatccttta tcgcaacaat
     1741 gggaatggga cttttacaga cgtcaccgaa aaggcgggcg tcggcgtcac gggttggtcc
     1801 acctgcgctg tttggttcga ctatgacggc gatggcaagc tggacctatt cgtgtcgagc
     1861 ttcattgagt ttgataggtc gcagcaccgg ctttgtgttg ataagacagt gacacaaaac
     1921 tactactgca ttccgcgcat cttcaagccg cgtccgagtc atctcttccg caacaacggc
     1981 aacgggacat tcactgacat gagccgagat tccggaatct ccggctcgcc cgggaagtcg
     2041 tttggcgcgg ttgcgaccga catcaacaag gatggcctaa tggacctctt tgtcgccaac
     2101 gacacgatgc ctaacttcct tttcatcaac aaaggtaacg gcaaattcga agagtcgggg
     2161 cttgcggcag gagtgtctta tggcgaggca gggacgccgc ggtcagggat gggcgtagac
     2221 gcggaagact acgatggcga cgggtggcag gaccttttcg tcgcgaacat cgatcaggaa
     2281 ttcttcagcc tctatcacaa ccaaatggac ctgaccttta ctgatcaacc cggcgagatc
     2341 gcgccgagca cacaattgct gagtgggtgg gggctgaagt tctttgatta cgacaacgac
     2401 ggcgaccccg atctcctgct cgccaacggc cacccggacg atatggtcga gactaagtct
     2461 gctcgagtga agtacaaaga accactcctg ttgtttgaaa acaccgggaa gacttatcga
     2521 aacgtaagtg cgctttccgg tgcggtgttc gctaagagtt ttccggcgcg tggtatggcg
     2581 gtgggagact tcgacaacga cggagacgtg gacgtgctcg tgtcaaacaa tggcgaggca
     2641 ccattgctgc ttcgcaacga gggtggaaat cgcaataact ggcttggcct ccacctcgtc
     2701 tcaacgaaaa gtaatcccgc ggctgttggc gctgttatta cctggcaggc tggcggggtt
     2761 agacgtagtc gcttgaagac aagtggcgga agctatctcg cctcgcacga tccgcgcgaa
     2821 gtgcttggct tgagatcagc agcgattgtt gagagtgtcg agattcgctg gccaagcggt
     2881 gtagtggata agctttctcg cctgccagtg aatacctacc tcaaggttgt cgagggccgc
     2941 ggtgtggtca agaattagcc gatagcgtta attggcggcc tgcttcgccg gcgctgccag
     3001 ggccttcacc tgcgccttcc atgcggctgg tgtcatatgt tgcagccgat gtaattccga
     3061 ggcggcgatc acgagttgtc ggacgccggg aatgattggg aattccagcc cgcccttggg
     3121 attggccgcc tgatcgagag ccgtgagccg ggcgttgcgc cactcaggag tcggagttat
     3181 gccccgagaa atgtaagaca aagcttccag tccaattgtc cccaactcat tcaagtcttt
     3241 agccaacggc tttacttcgt gcagagccgg cgacctgtcg atcatagctt ccaactcagg
     3301 gcccgcattg cgccactgcg tgagcgtagt tcgaacttgt tgtgagtggg cctcgaaacg
     3361 gggagcgtct gagagcaggc tatcgacagt cgccgcgaag cgtctcgctg cgtcgctgtc
     3421 cgggcgcgct gcatcgatga gaccagtaag cgggctgagc atagtctgcg gtctctgctg
     3481 gtagcgcctg tattccttaa ctggctcaac aacagacacc aacgtctgaa gcgggccgat
     3541 ttctctgcct tgggccaacc gtctgagcaa cattgcctgg ttcttctcgt gggtcaaacc
     3601 aacttcttcc agccgcaaac ttgttacggc gagacgtcgg tacatatcgt ccacgtcagt
     3661 tactgagcgc ggcgaccaga ggcgctcggc gatggccgcc gtgcgcggcc atattcgaga
     3721 gtctatcgtg tcaggtgtaa cccactcact ccacatcgtc gcctcgccgc cgagaacgcg
     3781 agccgcctcg gttgcggtta gtgtgctgtc agccggaatg gggtccacgg catagtgctg
     3841 cgaagtaggg aagatcaggt caatgtagta accagcggac agaacaccct tgtagccttt
     3901 cctcgctgcc tccgcgagtg cgcctgggcc gcgccatgag tgaatcacga cctcctgcgg
     3961 caaatcgggc tgaaagattt cgtcccaccc catcatgatc tttccgtgct tcttgaggat
     4021 ctgcagcgta cgtttgttga agtaagtttg gagagcatga ttatctttta tgccctttga
     4081 cttcataaaa gcctggatct gcgggttccg gtcccactgc ttgccctcgt tttcatcgcc
     4141 accgatatgc agataggcgt cggggaagag cccagccatc tccccaaaaa aagtgtcgag
     4201 aaatttatag acttcttcgc gggtggggtc gagcgccggc tcaaagattc cgggcttgcg
     4261 ttcaatcgta tatggacccg gagcactccc gagctcggga tgcccaacga gccaactggt
     4321 tgcatggccg ggaaggtcaa actcagggac gacgcgaatg ccacggtcac gtgcgtagga
     4381 gatgatctct ctcgcctgat cctgtgtgta gaagtgacca tcggagccaa gctggtggag
     4441 gcgcggaaat ttcttgctct ctatgcggaa gccctggtct tcagtcaggt gccagtgaaa
     4501 gacgttgagt ttcactgccg ccatcgcgtc gagattgcgc ttcaacacat ccatcggttg
     4561 aaaatggcga gcaacatcga tcagcaagcc acgccatggg aagcgtggcc gatcctgaat
     4621 c
//
LOCUS       DASUEX010000548         3747 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_528779, whole genome shotgun sequence.
ACCESSION   DASUEX010000548 DASUEX010000000
VERSION     DASUEX010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3747
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528779"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            225..560
                     /locus_tag="VFV61_09180"
     CDS             225..560
                     /locus_tag="VFV61_09180"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX4900692.1"
                     /translation="METSCQLLRVADALKKLPSAEGKRFATVFEHGTLAVEIYAPRGV
                     DPQQPHSRDEVYFVASGSGEYVCGESRQSFQPTDLLFAAAGVVHRFENFTDNLSVWVL
                     FYGPEGGEK"
     gene            557..961
                     /locus_tag="VFV61_09185"
     CDS             557..961
                     /locus_tag="VFV61_09185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900693.1"
                     /translation="MTEADNTPRLNSAAPCFAVANVGETIRWYEELLGFAGHRFPQHE
                     PHVFGILVRDRIEIMLQRVAGYQKPDLYSSRDGGVWDAYIRMRGVREFYDSIKDRVEI
                     IRPLQKQFYGDWEFEVIDPNGYVLVFSELIDE"
     gene            complement(1168..2943)
                     /gene="polX"
                     /locus_tag="VFV61_09190"
     CDS             complement(1168..2943)
                     /gene="polX"
                     /locus_tag="VFV61_09190"
                     /inference="COORDINATES: protein motif:HMM:NF006375.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HEX4900694.1"
                     /translation="MDKKLDKFAIAAALQEISALLELKGGVNRFKARAYQTGARAVAA
                     IAGDIGRALSENRLTSIPGIGNALASQIRQLHLTGTSSVLDKLKQEFPPGIIELSRLP
                     GLSTSKIAKLHEALGVTSITELQAAAAAGKVRNVPGFGAKTEQGLLEAIADHERDHKK
                     QKRLHLHHAERVADQVLSYLGTSKDLQEVSIAGSLRRGQETVGVIRLVASSKNPVAVL
                     KHLLRFPLIIKVAEQSDSSCSLELGEGATVSLRAVKPNQFGLALFWETGSEAHLTKIH
                     DIANRKGLALDRETLKLSIKTNGKAKSLQSRKRPLQLRTEAELYPLLGMQHIAPELRE
                     DEGEIEAALKKTLPAELVSLADIKGMIHCHTTYSDGKHSVAEMAHAAEAMGMQYLTIT
                     DHSPTASYAGGLKLDRLKRQWEEIDETQEQVKIKLLKGTESDILADGRLDYPDQILER
                     FDVVIASIHSRYKMDAEKMTKCLMTAMRQPIFKIWGHALGRLIQRRPPFACDVERILD
                     VIAESRAAIEVNGDPYRLDMEPRWIREARKRKIKFVISVDAHSMGAMNNLKYGVTMAR
                     RGWLQKSEVLNTLSVAKFRQAVRPA"
     gene            3164..>3747
                     /locus_tag="VFV61_09195"
     CDS             3164..>3747
                     /locus_tag="VFV61_09195"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX4900695.1"
                     /translation="MPIATINPATGETLQTFDELTTQELGQKLQLAADTFRSYRTTSF
                     ADRSERMLRAASLLEADKQKYARLMTLEMGKPIKGAVQEVEKCALVCRYYAENAERHL
                     ADQVVETNATRSYVRFQPLGPVLAIMPWNFPFWQVFRFAAPALMAGNVGLLKHASNVP
                     QCALAIEEIFARAGFANGAFQTLLIGSALVEQVL"
BASE COUNT          844 a          980 c         1000 g          923 t
ORIGIN      
        1 tcgaagctgc gaaggatcag tttgaacaaa agcttggtcg gcttaatgag gagtttgggg
       61 cttcggccac cttgccctgg gagtgatgta attattcttc tatcgtaggt gagatttcgt
      121 gttggcgtcg tgaatcgtga cttcctaccg gcaaacaaag acgatcaacg aaacagcgcc
      181 gaacactaat tttgaagaca gctcatttga caggttgatt agccatggaa acctcttgcc
      241 aattactgcg tgtcgctgac gcgcttaaaa aactaccaag tgccgaagga aagcgatttg
      301 ccactgtctt tgagcatgga acactcgcag ttgagatcta cgctccgcgg ggcgtcgatc
      361 cgcaacaacc acacagccgc gacgaggttt attttgtagc aagcggcagc ggtgaatacg
      421 tttgcggtga gagccgccag tctttccagc ccaccgactt gcttttcgca gccgccggcg
      481 ttgtgcaccg gtttgagaat ttcactgata atctaagtgt ctgggttttg ttctatggac
      541 cggagggtgg tgaaaaatga cggaggcaga taacactcct cgtctcaact ccgccgcacc
      601 ctgctttgcc gtggcaaacg tcggcgaaac tattcgctgg tatgaggagt tgttgggatt
      661 tgcggggcat cgtttcccgc agcatgaacc tcatgtcttc ggcattctcg tacgcgatcg
      721 aatcgagatc atgctgcagc gcgttgctgg ctaccagaag ccggatctct acagcagccg
      781 ggacggcggc gtttgggatg cttatattcg tatgcgagga gtgcgagagt tttacgattc
      841 aatcaaggat cgcgtggaga taataagacc gctacagaaa cagttctatg gagactggga
      901 atttgaagtg atagatccga atggatatgt tttggtattt agtgagctga tcgatgagtg
      961 acggtgggtt ttagacgcga tctggagagg gatcccaagc gtcttaagct gaccattctc
     1021 attctcgccg ggcttcagcc cggcgccaag actcacttaa ttagacgaga aaccgtttaa
     1081 acggtttccc caattcttac gcggcaaaca ccgcgctaaa gcgcggtgag aatgaaatgc
     1141 ccaaagtggg ctttgcgcat attaacctca ggcgggtcgc acagcttggc gaaacttcgc
     1201 cactgacagt gtattcaaca cctcactctt ctgcagccag ccgcgccgcg ccattgtcac
     1261 gccgtatttc aagttattca tcgctcccat tgaatgagca tcaactgaga ttacgaactt
     1321 gatcttgcgc ttgcgtgctt cgcgaatcca gcgcggttcc atgtcgagcc ggtagggatc
     1381 gccattcact tcgatggcag cacgcgactc ggcaatcaca tccagaattc gctcgacgtc
     1441 gcaagcaaac ggcggtcgtc tttgaatcaa ccgacccagg gcgtgtcccc agatcttaaa
     1501 aatcggttgg cgcatcgccg tcatcagaca cttggtcatc ttttcggcgt ccattttgta
     1561 ccgcgaatga atactggcga tcaccacatc gaaacgttcc aaaatctgat ccggatagtc
     1621 gagcctgccg tcggcgagaa tatccgactc agttcccttc agaagtttga tcttaacttg
     1681 ctcctgagtc tcatcaatct cttcccattg gcgcttgaga cgatcgagtt tgagtccgcc
     1741 ggcataagac gcggttggtg agtggtcagt gatcgtgagg tactgcatac ccatggcttc
     1801 cgcggcgtgc gccatttccg cgaccgaatg tttgccatcg gaataagtcg tgtggcagtg
     1861 aatcattcct ttgatgtccg ctaagctgac caattcagca ggcagcgttt tcttcaacgc
     1921 cgcttcaatt tcgccttcat cttcccggag ttcgggagcg atgtgctgca tacccagcaa
     1981 aggatagagc tcagcttcgg tccgtaattg cagcggcctt ttgcgagact gcaacgactt
     2041 cgccttgcca ttcgtcttga tacttagctt caaagtctca cggtctaggg cgagaccctt
     2101 gcggtttgca atgtcgtgga tctttgtcag gtgtgcttcg gaaccggtct cccagaacag
     2161 agcgagccca aactgattgg gcttaactgc tctcaacgac accgttgctc cttcgccaag
     2221 ctctaacgag caactcgagt ctgattgttc tgcaacttta atgatcaacg ggaaccgcag
     2281 caggtgcttt aataccgcga cggggttttt agagctcgct acgaggcgaa tcacgcccac
     2341 cgtttcctgt ccgcgacgca aggatccggc aatgctcacc tcttgcaaat cttttgaggt
     2401 tcccaggtaa ctcagaacct gatcggcaac gcgttcggcg tgatgcagat gaaggcgttt
     2461 ctgtttctta tgatcgcgct cgtggtcggc aatcgcttcc aaaagcccct gctccgtttt
     2521 cgcgccaaag ccgggaacgt tgcgtacctt acccgctgct gccgctgctt gcagttcagt
     2581 gattgaagtg acgccgagtg cttcgtgcag cttcgcgatt ttgctggtac tcaagccagg
     2641 caggcgtgaa agctctatga tgcccggagg aaattcttgt tttagtttgt cgaggaccga
     2701 agaagtaccg gtgagatgca gttggcgaat ttgggaagcg agggcattac caattccggg
     2761 aatggaggtg agccgatttt ctgaaagggc gcgaccaatg tcgccggcga ttgccgccac
     2821 cgcgcgggcg ccggtctgat aggcacgcgc tttgaaacga tttacgccac ctttcaattc
     2881 caacagcgcg ctgatttcct ggagcgccgc ggcaatcgca aacttgtcga gtttcttatc
     2941 catggaggtt gagaatacct tagacgtttt tcaatttgat ggcgactaac cgatattacc
     3001 gccaaggcgc agaggcgcag aggcgcagag gcgcagaagg aaaaaaggat agtgaagagt
     3061 tagtacttca actactctaa aaaacttctt ttctttttga ttttgttttc ttggcgtctt
     3121 ggcgcctctg cgccttggcg gtaaagtgca ttaaggaaaa gctatgccga tcgcaactat
     3181 caaccccgct accggggaaa cgctgcaaac ctttgacgag ctgacgactc aggagctcgg
     3241 acaaaaactc caactcgccg cggacacttt ccgtagttat cgaacaacgt cgttcgctga
     3301 tcgctccgaa cggatgttgc gagccgcttc attgctggag gccgacaaac agaaatatgc
     3361 gcggctgatg acgctcgaga tgggaaaacc aatcaagggc gcggtgcagg aagttgaaaa
     3421 gtgcgctctc gtttgtcgct actacgctga gaacgccgag cgacatcttg ccgaccaggt
     3481 tgtcgagacc aatgcaacgc ggagttacgt tcgttttcaa ccactcggac cagtactggc
     3541 gataatgccc tggaactttc ccttctggca ggtgtttcgc ttcgcagcac cggctctgat
     3601 ggccggcaac gtcggcttgc taaagcatgc ttcaaacgtt ccccaatgcg ccctcgcgat
     3661 tgaggaaatc ttcgcgcgcg ccggttttgc caatggcgcc ttccaaactc tgttgattgg
     3721 atcggcgctc gtagaacaag tcttgaa
//
LOCUS       DASUEX010000549         2707 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_656037, whole genome shotgun sequence.
ACCESSION   DASUEX010000549 DASUEX010000000
VERSION     DASUEX010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2707
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_656037"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1692
                     /locus_tag="VFV61_09200"
     CDS             <1..1692
                     /locus_tag="VFV61_09200"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX4900696.1"
                     /translation="GGSTIGVLNLGYRVEDAFSEADIELLVHIGGQIAIAVQNSVNFE
                     RARKFEQRAARERDRIRLLLEINNAVVSHLDLKEVVKTVSASLREIMPHDAAGIALYE
                     PEPEQLREYTNVNYKDLESFRVGDTIPLEGTPAGHVFLTGQPLLVRRPNLTAYPADRY
                     SQHPVEGSPKSACLAPLISHGRKLGIAGVSSTQEEKFTDEDLEFFSQISGQIALAVEN
                     SLQYREIEALKNMLTGEKHYLEEEIKTQYNFAEIVGQSAALREVLRQVETVAPTDSTV
                     LLIGETGTGKELIARAIHNLSARNERTLVKLNCAAIPTGLLESELFGHEKGAFTGAIA
                     PRVGRFELAHKGTLLLDEIGEIPLELQPKLLRVLQEHEFERLGSSRTIKTDARLVAAT
                     NCDLAEMVEEKKFRSDLFYRLNVFPIMIPPLRERTGDIPLLVSYFTQKHAGRMNKKIQ
                     SIPAQTMEALSSYHWPGNVRELENFVERSVILSRGSELQSPLKELQSSLDVSGDGQSS
                     PTGSKLTTMDEMERAYIEEVLRHTNGSIGGKGGAAEILGMPASTLRGRMKKLGVR"
     gene            complement(1768..2316)
                     /locus_tag="VFV61_09205"
     CDS             complement(1768..2316)
                     /locus_tag="VFV61_09205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900697.1"
                     /translation="MKLKAGVILVLLLLGWTANVVAQQATNAMENTETLRMQLLEVES
                     KQQQLRMRLQLLDENLKPENIERALVGIGSTRPEDLRASRRDLLTIERDGVVAQLKIL
                     EASHARLEAEIASAEVQAYLRLVQTPPPAANHLSLFGSVQPRYGQIARWYGRTLVSAS
                     ITLALLVVATVVITKARFFCSE"
     gene            complement(2509..>2707)
                     /locus_tag="VFV61_09210"
     CDS             complement(2509..>2707)
                     /locus_tag="VFV61_09210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900698.1"
                     /translation="PCARVRPKANDEVDHAAKWLWKKEDKELSMVNRPKRKRETILDF
                     PTRNTARYAKYRVEGFMPPPL"
BASE COUNT          657 a          741 c          706 g          603 t
ORIGIN      
        1 ggaggctcta cgatcggagt tctgaacctc ggctaccggg ttgaagacgc cttcagcgaa
       61 gctgatattg agttgctcgt acatatcggc ggccagattg cgattgccgt acaaaacagc
      121 gtcaactttg aacgggcccg caaattcgag caacgtgccg cgcgagagag agatcggatc
      181 cgcctgttgc tggaaattaa caatgcagta gtttcgcacc ttgatctgaa ggaagtagtc
      241 aaaaccgtct cagctagttt gcgcgagata atgccgcacg acgctgccgg aatcgcgctt
      301 tacgagccag agccggagca gttgcgggaa tacacaaatg ttaactacaa agacctcgag
      361 tcttttcggg tcggcgatac gatccccttg gagggaacgc cggccggaca cgttttcctc
      421 accggtcaac cgcttctggt aaggcggccc aacctaactg cctaccccgc tgatcgctat
      481 tcgcagcatc ccgtggaggg cagtccaaag tcagcttgtt tagcgccgct catctcgcac
      541 ggacgaaagc taggcattgc cggcgtcagc agcacgcagg aagaaaagtt taccgacgaa
      601 gacctggagt tttttagcca gatctccgga caaatcgccc tcgctgtaga aaacagcttg
      661 cagtaccgcg agatcgaagc actaaagaac atgcttaccg gtgagaagca ctatctcgag
      721 gaagagatca agacgcagta taacttcgct gaaattgtcg gccagagtgc ggcgctgcga
      781 gaagtcttgc gccaggtcga aacggtggcg ccgactgact caacggtcct gttaatcgga
      841 gagacgggca ccggaaaaga gttgattgcg cgcgcgattc acaatctcag cgcgcgaaac
      901 gaacgcaccc tggtgaaact caactgcgcg gcgattccga cgggactcct ggaaagcgaa
      961 ttgtttggcc atgaaaaagg cgcgttcacc ggagctatcg cgccacgcgt cggccgcttc
     1021 gaactagcgc acaaaggaac gctgctgctt gatgagattg gtgaaatccc tttggagtta
     1081 caaccaaagc ttttgcgcgt tttgcaggag catgagtttg agcggctcgg cagttcgcgc
     1141 acgatcaaga cggacgcgcg cctcgtggcg gcgactaact gcgatctggc agagatggtt
     1201 gaagagaaga agttccgtag tgatctgttt tatcgcctca atgtttttcc gattatgatt
     1261 ccgccgctgc gggagcgtac tggcgacatt cccttgctcg taagctactt cacgcaaaaa
     1321 cacgcgggcc gaatgaacaa aaagattcaa tccatacccg cgcaaactat ggaggccctc
     1381 agcagctatc actggcccgg caacgttcgc gaactcgaga atttcgttga acgctcagta
     1441 attctttcgc gcggctctga actgcagtcg ccattgaaag aactgcagtc atccttagac
     1501 gttagcggcg acggacaatc ctcacccacc ggatcaaagt taaccactat ggacgagatg
     1561 gagcgggcct atatcgaaga agttctccgc cacaccaacg gcagcatcgg tggcaaaggc
     1621 ggtgctgcgg aaattctcgg gatgccagct tccacgcttc gtggccgcat gaaaaagctc
     1681 ggcgttcgct agcgaagact cggatcccaa gggtaattta acctgcgctc ggcgtgtcac
     1741 agccactgcc tcacattcga cggcaaacta ttccgaacaa aagaatctcg ctttcgttat
     1801 taccacagtc gcaaccacta acaaagcaag cgtaatagat gcggatacta gtgtccgtcc
     1861 gtaccacctt gctatttgcc cgtagcgtgg ctggacggat ccaaataatg acaaatggtt
     1921 cgctgcgggc ggtggtgtct gaacaagccg caggtaggcc tgcacctcgg cggaggcgat
     1981 ctccgcctct aaacgtgcgt gactcgcctc aaggatctta agctgcgcca ctactccgtc
     2041 cctttcaatt gttagcagat cgcgacggga ggcccgcaga tcttcgggcc gggtggaacc
     2101 gatcccgaca agtgcccgct cgatattctc gggtttaaga ttctcgtcta atagctgcag
     2161 ccgcatacgc agctgttgtt gtttagactc aacttcaagt aactgcatcc gcaaggtctc
     2221 ggtattctcc attgcgtttg ttgcctgctg ggcaacgaca ttggcagtcc atcccaacag
     2281 caaaagcacc aaaatcactc cagcctttaa cttcataaag attctcctga acaaagtatc
     2341 cgcaagtcag attcttttaa ctcggattga ctaacgtctt acgctactgc aacagatggg
     2401 ccaagacggc caattcgtct tttgcctcct cgcaacctga atgagttcta aataatccgt
     2461 tgtaatccgc acgtatggtc aagaaataga tggtaagcgc caacttgttc agaggggggg
     2521 cggcatgaat ccttcgacgc ggtatttggc gtagcgcgcc gtattccgcg tcggaaagtc
     2581 caggatggtt tcgcgctttc gttttggccg attaaccata ctcaattcct tatcctcttt
     2641 cttccataac catttagctg cgtgatccac ttcgtcattt gcttttggcc tcacccttgc
     2701 acaagga
//
LOCUS       DASUEX010000550         2017 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1598099, whole genome shotgun sequence.
ACCESSION   DASUEX010000550 DASUEX010000000
VERSION     DASUEX010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1598099"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1549
                     /locus_tag="VFV61_09215"
     CDS             <1..1549
                     /locus_tag="VFV61_09215"
                     /inference="COORDINATES: protein motif:HMM:NF016845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HEX4900699.1"
                     /translation="GNIVVTIAIRLHENLLAGETGKLTAAGLTNYDTVWISKAALQDS
                     SGHGGFYRALFSEPEQTWSFGKAATGHKPDNIDWRLNTSASSGFRSLSRVADDPLAQV
                     RVATVTISVASPDGGTYVWDRIAPDSDYTNAQGPNSLSSCFGEKLEGIRARVLPLVVL
                     VGEELATGVDVVETILSGVGSGEPADTAQFERLDWLDDDTFSLRRTAELSGGNDGQLP
                     GVEEYLGRATEEDDWRTGLSALEFVDEVSIVAAPGATAGYLNNSDRANAIIAGLVAHA
                     ERLRHRIAVIDSGDKQTMPEVRAMRGLIDSKHAAFYYPWIRVLDPLTQKEISLPPSGI
                     VAGIYARVDRERGVFKAPANEVVHGATGLEVILDDARQEILNREGINCFRFFPDRGFR
                     LFGARLASSDPEFKYVNVRRYIAYLEHSIDRGTQWAVFEPNGEILWANVRAAIEDFLL
                     LEWRSGALMGVKPEEAYFVKCDRSTMTQADIESGRLICLIGIATVRPAEFVIFRIGQW
                     TASRPDP"
BASE COUNT          441 a          533 c          590 g          453 t
ORIGIN      
        1 cggcaacatt gtggttacta ttgctatccg tctccatgaa aaccttctgg ccggagagac
       61 tggaaaactg accgccgcag gccttaccaa ttacgacacc gtctggatca gtaaggcggc
      121 cctgcaggac tcttcgggcc acggcggctt ttaccgcgcg ctgtttagcg aaccggagca
      181 gacttggagc tttggcaaag cagccacggg acataagcca gacaacattg attggcgcct
      241 taacacttcg gcctcctctg gtttcagatc actttcgcgc gtggctgacg atcccctggc
      301 gcaggttcgc gtcgcgaccg taacgatctc ggtcgcatcg cctgatggcg ggacgtatgt
      361 ctgggacagg attgcgccgg actctgatta cacaaatgcg cagggcccga attccttatc
      421 aagttgcttc ggagagaagc ttgagggaat ccgggcgcgc gtactgccgc tggtggttct
      481 ggtcggagag gagctagcta ctggagttga tgtcgtggag acgattctga gcggcgtagg
      541 ctccggtgaa ccagcagata ctgcgcagtt cgaacgactc gattggcttg atgacgacac
      601 gttcagcctt cgtcgcactg cggagttgag tggtggcaac gacggacagc ttcccggggt
      661 cgaagaatac ctgggacgtg cgactgaaga ggacgactgg cgaacgggtt tgtctgcgct
      721 tgaatttgta gatgaggtgt cgattgtcgc tgctccgggc gctaccgctg gctacctgaa
      781 caacagcgat cgcgcgaacg ctatcattgc cggtttggtt gcgcacgcgg aacgcctccg
      841 ccatcgcatt gccgtgattg actccggcga taagcaaacg atgcccgagg tccgcgcgat
      901 gcgaggattg atcgattcga aacacgcggc attctattat ccctggattc gtgtactcga
      961 cccgctcacg cagaaggaaa tcagtctgcc gccgagcggc atcgtagctg ggatctatgc
     1021 acgcgtagat cgcgaacgtg gcgtcttcaa ggctcccgcc aatgaagtgg ttcatggagc
     1081 aactggtctt gaggtgattc tggacgacgc tcggcaggaa atcctaaaca gagaaggcat
     1141 caattgtttt cgattcttcc cggatcgtgg ttttaggctc ttcggggcgc gactcgcgag
     1201 ttccgatccg gaattcaagt acgtaaatgt gcgccggtac attgcttacc tcgaacactc
     1261 gattgacagg gggactcagt gggctgtctt cgaacccaat ggtgaaattc tgtgggctaa
     1321 cgtgcgcgca gccattgaag attttctcct gctggaatgg agatcgggcg cactgatggg
     1381 agttaagcca gaagaagcat acttcgttaa atgcgaccgc tcgacaatga ctcaggccga
     1441 catcgaaagt ggtcgcctga tctgcctcat cggcatcgca acggtgcgac ctgccgagtt
     1501 tgtgattttc cgtatcggtc aatggacggc cagcagaccg gatccatgaa catgccaagt
     1561 agtgggtttg gagccttcat cctcgcagtt taactttacg gtcaatcttg ggtaacggcg
     1621 gtgcggatgc ggtcgtcggc gcctgcctcg aggagtgctg tctgactaat cgtgacggga
     1681 aagcccggcc tgcagcagcg agaacgataa gtctaaaagg cgtgcggcca aagaccctca
     1741 ccctgaaata aagaagccca aattcgttga aaattgttga ctcccgggat gccaggagta
     1801 gaatgtcgtc gcaccaacta ctgacgatag taggctccac ctcatgtcca cgggcgatgt
     1861 tttcgccctt cgcaagcacc acccgacaaa ccttgaaaag gagacgccgt gtactacttg
     1921 ctaaggaatt ccctcccact gctcgtctta ttgagcctaa ttggtctggc catgactgtg
     1981 gttcctccgg tcagtcaaag tcaggataag gatattg
//
LOCUS       DASUEX010000551         2242 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_779693, whole genome shotgun sequence.
ACCESSION   DASUEX010000551 DASUEX010000000
VERSION     DASUEX010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_779693"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="VFV61_09220"
     CDS             complement(<1..362)
                     /locus_tag="VFV61_09220"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEX4900700.1"
                     /translation="MKQNSLICGKVLRAAAVASLCLLSVLASATFVAAQDAATGKVTG
                     TIKDQRGANVSGAEISLLHQQRAVVATTTSDGNGNFTLDNVAPGYYEVKVVSAGFTEF
                     HKAVAVTSNATVELAVSL"
     gene            complement(622..1629)
                     /locus_tag="VFV61_09225"
     CDS             complement(622..1629)
                     /locus_tag="VFV61_09225"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX4900701.1"
                     /translation="MFFLLGLSIVLAALLLCNSGTSLISSLTWRIFGPRAVGRLSAAS
                     CASIVFLLRTFPIVLGIVCVCLLLIPAYLEHEPRDAHEPVSLKLALIACASAIGLALA
                     LFRGLAAWRATARLNSDWLRHAEPINVAQVRIPSYRIQHQFPVIAIVGVLRPRLFIAE
                     QVLASLEQKELIAAIEHEAGHVVANDNLRRGLMRACRDVLVMIPSGRLLDRAWVEASE
                     AAADEYAAGRGRGVALDLASALIKIARLIPAGMKPAMPAGAFLVSTEEEASGVKARVR
                     RLMQLADHQRTQPGRALISRMPLWIPIVLTILVATLAANEPHVLASVHLLIEESVHLL
                     S"
     gene            complement(1631..2071)
                     /locus_tag="VFV61_09230"
     CDS             complement(1631..2071)
                     /locus_tag="VFV61_09230"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HEX4900702.1"
                     /translation="MKSPRFLLRGFRRPSELASLALGRLERQVLEETWRRGETSVRDI
                     VNTFNEQVAYTTVMTTLDRLYKKRLLKRRKDGRAFIYSPLVTRAEFEQGIAEDVIDGL
                     LGGGASGAEPVLACIVDAVSEHDRELLDELDRLIKEKRAELREN"
BASE COUNT          503 a          626 c          637 g          476 t
ORIGIN      
        1 aacagcgaca cggccagttc cactgtggcg ttactggtaa cagcaacagc tttgtgaaac
       61 tcagtgaagc cggcactaac aactttcacc tcgtagtaac ccggagcgac attgtcgagg
      121 gtgaagtttc cattgccatc ggaagtggtg gtggcaacga ctgcgcgctg ttgatgaagc
      181 agcgaaattt cagcgccgct tacatttgca cctcgttgat ccttgatcgt gccggttact
      241 ttaccggtgg ccgcgtcctg tgccgcgaca aacgttgcac ttgccaacac ggatagaaga
      301 cacagggacg cgaccgcggc ggcgcgcaag actttcccgc aaattaaact gttctgcttc
      361 attgttgtta tcagcgcccc tccgccttct gggccggggg cgatactcct ggataataca
      421 gctgagaatt tcgttttgtg agtctggttt ggagtggcga cacagccgca gaccgtgtcg
      481 ccaccggcac ccgcaaaact acaccttgct tccctgcggg attctttgag aacggagcta
      541 aaggcgacca gcgtacacgg ggcgtcgaac tactactaga cttcgtagtt cgcagagaca
      601 aagcgtttcc cttgaggttg tttaacttag gaggtgaact gactcttcaa tcaggagatg
      661 aacagaagca agcacatgcg gttcattggc ggccagggtg gcgaccagaa tcgtcagaac
      721 aatcgggatc cacaaaggca ttcgcgaaat caaagcgcga cctggttggg ttcgctgatg
      781 gtcggctaac tgcattaagc ggcgcacgcg agctttgact ccgctcgctt cttcttcagt
      841 gctgacgaga aatgctcccg caggcatcgc cggcttcatt cccgcaggaa ttaatcgcgc
      901 aatcttgatc agggccgagg cgagatccag ggccactccg cgtccgcgcc cggccgcgta
      961 ttcatcggcc gcggcctcgg aggcttcaac ccaggcacga tccagtaggc ggccactagg
     1021 gatcattacc agcacatcgc gacaggctcg catcagaccc cgtctcaagt tgtcgttcgc
     1081 cacaacatga ccggcttcat gctcaatggc ggcaattagt tctttctgct ccagcgaagc
     1141 gagaacctgc tccgcaataa agagccgggg tcgcagcacg cccacaattg cgatcacggg
     1201 gaactggtgt tggatgcggt atgaaggaat ccttacctga gcgacattga tgggctcggc
     1261 gtgtcgcagc caatcggagt tcagccgcgc tgttgcccgc cacgctgcca gtccccgaaa
     1321 caacgcgagc gcaaggccga tagcagaagc gcaagcgatg agcgccagct tgaggcttac
     1381 gggttcatga gcatcgcgcg gttcgtgttc gaggtaagcg ggaattagca gcaggcacac
     1441 gcaaacgatt cccagcacaa tcggaaatgt gcgcaataag aaaacgatgc tggcgcaact
     1501 cgccgcggaa aggcggccga ctgcacgcgg tccgaagatt ctccatgtaa gtgaggagat
     1561 caaggacgtg ccgctgttgc agagcagcag cgctgccagc acgattgaga gtccgagtaa
     1621 gaaaaacact ctagttctcc cgcaattcag cccgcttttc cttaatgagg cggtcgagtt
     1681 cgtcgagcag ctctcggtcg tgttcgctaa ctgcgtccac gatgcaggca agtactggtt
     1741 ccgcaccgga agcgccgccg ccgagtaggc cgtcgatgac gtcttcggcg attccctgct
     1801 caaactcagc gcgcgtcacg agcggcgaat aaataaaagc ccgcccgtct ttgcggcgtt
     1861 tcaagagcct cttcttgtaa agccggtcga gcgtcgtcat caccgtcgtg taagcgacct
     1921 gctcattaaa agtgttgacg atatcgcgca cgctggtttc gccacgccgc cacgtctctt
     1981 ccaaaacctg acgttcaagc cggccgagcg caagcgaagc aagttcactg ggtctgcgaa
     2041 atccacgaag caggaatctt ggacttttca tgagggcgga attgttgttc ggcgatggtt
     2101 gccctgtcaa tgatcaatgg aatcattggg cttgggtctt tggtctttgg ccttgggtct
     2161 tgggtcttgg gtctttgggg gttgcggttt gaatccagta gtgaatctca actgaagtca
     2221 aagctcagga aaccaaaaac cc
//
LOCUS       DASUEX010000552         2778 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_656239, whole genome shotgun sequence.
ACCESSION   DASUEX010000552 DASUEX010000000
VERSION     DASUEX010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_656239"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            325..1695
                     /locus_tag="VFV61_09235"
     CDS             325..1695
                     /locus_tag="VFV61_09235"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX4900703.1"
                     /translation="MFLKKSTSLIVATLCFCLVSTAPCPRALAQKKKAPVEATPPPVR
                     EEFKFGKTDLEVFEQSELLDAKFERDGLVLQDEAANAYVRRIGQALVPKGLELERVSW
                     RFRVLRDPQPNAFALPNGSIYVSTGLIFLVDNESQLAAIIAHELTHVMRRHTYVQNRS
                     NRKKFLTMNIMAAVGAYAPGGIVGAVITVVTAVAPFIVMATVFGYSRDLEREADLKGV
                     DMMISAEYAPEEMVNVMKLLDKDIEGENIRLFYNDHPSLNERIKYLSSYLGARADRVT
                     HQMELNRERTAYFKTMESAMRHNLQLSMMAGRFRSAVFHAQRLVDFHPDSENLFWLAE
                     AYRTLGPRSPQLTGKELTNSAKKDAAKKRQKRTLEEEERELLETPVGKDNWQRHQQKA
                     EELYLRALNAENPAPAVHRGLGMLYEKLGRATDAASEYEKYIELVPTAIDRERIKRRI
                     ETLRRT"
     gene            1758..2426
                     /locus_tag="VFV61_09240"
     CDS             1758..2426
                     /locus_tag="VFV61_09240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900704.1"
                     /translation="MAIATLLLMGFSFPIHAQFLHPKIKSKETTIRTVVVLPAKVSVV
                     RDSMKGPEGMAAESDELSARAEKTIAEVLSKQKNVKTLSPPTAASVQDNSQPNYNVAD
                     FQTKFDDLLPKIMKKRSDVKKGRFTMGDEVLNLNLDKGTDAIVFIRGQGKKLTGGKTA
                     FTLLVGGTPAYLKLMIGIVDARNGDVLLYTDPMFGGDPTTAVDRLRKAMEKGFKKLPA
                     AAAH"
     gene            2475..>2778
                     /locus_tag="VFV61_09245"
     CDS             2475..>2778
                     /locus_tag="VFV61_09245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900705.1"
                     /translation="MRLRLIALTMVLGLVFAAALSSGRQAASGQQQNIRKQPACTMYC
                     GDGPDTFSLGAKPCWGGPLPAGGAGDHYRSLSEEDQKAICKNLAGKSNASCPAANAI"
BASE COUNT          708 a          705 c          748 g          617 t
ORIGIN      
        1 atcacgggac ttaaaatccg ttgtccgtgc gggacgtgcg ggttcgactc ctgccctcgg
       61 caccatcact agtctcagag tttcccgatt tccatcaaat ttatacttgg atgatctcaa
      121 gcccaaaagt aatgatgggt cctgatagga gacattcgta gttaagaaag gtggcggcaa
      181 gctacttctt acctccttcc taattcattt aagttcaacc ggtcggacgc accaacaacc
      241 taagaacaga tcttgactct ctcgataaat tcgacgaaag tcaggttcgc cggcgcttca
      301 cgtccaaagg gtttcgcctg gtttatgttc ctcaaaaagt ctacaagttt aatagtcgcg
      361 actctctgct tctgcctggt atccaccgcg ccgtgtccac gcgcgctggc gcaaaagaaa
      421 aaagcgcctg tcgaagcgac cccaccgccc gtcagggaag agttcaaatt tggaaaaacc
      481 gatttagagg tttttgaaca gtctgagctg ctcgacgcta agtttgagcg ggatgggctc
      541 gtgctgcagg acgaagcggc aaatgcttat gtacgtcgga tcggacaagc gttggttccc
      601 aagggactcg aactagagag ggtatcgtgg agatttcgcg tgctccgcga cccgcagccg
      661 aacgctttcg cactcccgaa cggctcgata tacgtcagca ctgggttgat tttcctggtc
      721 gacaacgaat cacagctggc tgcaatcatc gcgcatgaat tgacccacgt tatgcggcgg
      781 cacacctatg tccagaatcg tagcaaccgc aaaaagtttc tcacgatgaa catcatggct
      841 gccgtcggcg cctatgcacc cggagggatt gtgggagccg taatcactgt ggtgacggca
      901 gtcgctccct ttattgtgat ggccacggtc ttcggttaca gccgagacct ggaacgtgag
      961 gctgatctca aaggtgtcga catgatgatc agcgccgaat acgcgccgga agaaatggtc
     1021 aacgtgatga agttgctgga caaagacatc gaaggcgaga acatcagatt gttctataac
     1081 gaccatccgt cactcaatga gcgaattaag tatctcagct cgtatctcgg cgcgcgggct
     1141 gacagagtga cgcaccagat ggagttaaat cgggagcgaa ccgcctactt caaaaccatg
     1201 gaatcggcga tgcgtcacaa tcttcaattg tcgatgatgg ctggacgttt tcgttccgcg
     1261 gtctttcacg cgcaacgtct ggtggatttt catcctgatt cggaaaacct gttttggttg
     1321 gcagaggcct atcgtacgct tgggcctcgt tccccacagt taacaggaaa agaactaacc
     1381 aacagcgcta agaaagatgc ggcgaagaaa cgccagaagc gcacgcttga agaagaagag
     1441 cgcgagctgc tcgagacgcc ggtgggtaag gacaattggc aaaggcacca acaaaaggcc
     1501 gaagaattgt acttgcgcgc actaaacgct gagaaccccg cacccgcggt gcatcgcggg
     1561 ctgggcatgc tttatgaaaa gctcggccgt gccaccgatg ccgcttccga atatgagaag
     1621 tacattgagc tggtcccgac cgcgatcgat cgcgaacgaa tcaaacgacg cattgaaaca
     1681 ttaaggagaa cctaaaattg aggctcacta gttcctcccg tgttttccgg ttgcgagcgt
     1741 taatccgagc attaatcttg gcaatcgcaa cgttgctgct catgggtttt agtttcccaa
     1801 tccacgcgca gtttcttcat cctaagatca agtcgaaaga aacgaccatc cgtacggttg
     1861 ttgtccttcc cgcgaaagtt agtgtggtcc gcgatagcat gaagggcccc gaaggaatgg
     1921 cagctgagtc tgatgaactg agcgcgcgcg cagagaagac gatcgctgaa gttctttcaa
     1981 aacagaaaaa cgtgaaaacg ttatcaccgc caactgcggc atccgtccag gacaactctc
     2041 agccgaacta caatgtcgca gattttcaaa ccaagtttga tgatctgcta cccaagatca
     2101 tgaaaaagcg tagcgacgtt aaaaaaggtc gcttcacaat gggcgatgag gtgctgaacc
     2161 tgaacctgga taagggtacg gacgcgatag tcttcattcg tggccaaggg aagaaactaa
     2221 cgggcggcaa aacagccttt acgttgctcg ttggcggaac accggcttat ctgaagttga
     2281 tgatcgggat agtggatgcc agaaatggcg acgttctgtt gtataccgat cccatgttcg
     2341 gcggtgatcc aaccacggcg gtcgatcgac tgcgaaaggc aatggaaaaa ggattcaaga
     2401 aactgccggc tgctgccgcg cattgatttg tgtttgattt ttctgcttcg taagcccgac
     2461 cgttcggaga aatcatgaga ttacgactaa tcgcactgac gatggttttg ggattagttt
     2521 tcgccgcggc actgagttct gggcgacaag ctgcttcggg ccagcaacag aacattcgca
     2581 aacaacctgc gtgcaccatg tactgtggtg atggccctga tacgttcagc ttgggggcaa
     2641 aaccctgctg gggcgggccc ctgccggcgg gcggggctgg tgatcattac aggagtctct
     2701 cagaagagga tcaaaaggcc atctgcaaaa acctggcggg caagtccaat gcctcttgtc
     2761 cggcagccaa tgcaattg
//
LOCUS       DASUEX010000553         4291 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_909267, whole genome shotgun sequence.
ACCESSION   DASUEX010000553 DASUEX010000000
VERSION     DASUEX010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4291
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_909267"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1698
                     /gene="ggt"
                     /locus_tag="VFV61_09250"
     CDS             <1..1698
                     /gene="ggt"
                     /locus_tag="VFV61_09250"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX4900706.1"
                     /translation="LVSAFPLVLQPPQPTALAASRQPVRARHGIVAATNQLGERVGVQ
                     ILQSGGNAIDAAIGVAFALAVTHPAAGNLGGGGFMMIRLSNGKTTAIDYREMAPAAAH
                     RNVYLDKDGNLIKGEGSSTVGYRASGVPGTVAGLELALKKYGSGKLTWAQLVEPARRL
                     ASEGFPMGYGLARSLNRNEYLAKYEETRRIFLRNGNYYQEDEIFRQPELAATLTRLQR
                     SGPKEFYQGRTAQLIAADMKRNNGLITMADMRGYVAKERQPLQGSYRGYPVISMPPPS
                     SGGAVLLEMLNILEGFELDKYDWASSERYHLTTEAMRRAFADRAEYMGDADFVKVPVA
                     GLIDKSYAVKLRQSINFERASSSTEVRAGQPMGYESEETTHFTVVDVQGNAVANTYTL
                     NDSFGSGVVAKGTGVFMNNEMDDFAAKPGTPNLYGLIQGERNAVAAGKRPLSAMTPTF
                     VLRKDGSLWFTTGSPGGPTIINTVLLVITNVIDYKMNIQQAIDAPRIHHQWLPDQLVF
                     EPYGLSGDTQKALLARGHKFAKPTYLGNAEGIMIEEKTGVRLGASDPRRSDGQAVGY"
     gene            1925..3232
                     /locus_tag="VFV61_09255"
     CDS             1925..3232
                     /locus_tag="VFV61_09255"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HEX4900707.1"
                     /translation="MSLPDVLETEEQKLLACVHCGLCLEACPTYTLTGDENDSPRGRI
                     YLMRAVAEERLRSDSVTFKTHIDRCLGCRACEPVCPAGVEYGQLLEASRAAIFTPEVP
                     KGFRYRLLRFALRHVWLQPRRLRFSFALARLLRDTGLAKLLLQSRVIGLFSKRGEFGL
                     ALLLSSAPQIDTSAGKGLSEPAADEQTQTALLFTGCVTAGLFGRVNEATKRVLLVNGV
                     QAEAAPHQVCCGALHAHAGDLEGARLLARQNISAFGAGDTPVITNAGGCGAMLVSYGH
                     LLADDSTFAERARTFSARVRDVSQQLETKTIVNGAHLDGAVTYDASCHLLNGQHAAEA
                     PLKVLRAIPDLNFVPLEGAERCCGGAGVYNLLEPEMSQGVLAEKLAHVRETGATVLAT
                     GNPGCQMQIGAGLQVKGMELRTCHPVELLDMSYRQAGYYSEDS"
     gene            3235..3606
                     /locus_tag="VFV61_09260"
     CDS             3235..3606
                     /locus_tag="VFV61_09260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900708.1"
                     /translation="MSQRSANHITLAIFAPLLIVVGVAGFLVPAHLSLTSGAPAYNVF
                     HICFGLLGLLVLWARKERWISLFNAGFGLIDLFQALASYTDLTPKQYFLWTRVDDILH
                     VVIGLTLLIIGCYGLVREKKL"
     gene            3770..4228
                     /locus_tag="VFV61_09265"
     CDS             3770..4228
                     /locus_tag="VFV61_09265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900709.1"
                     /translation="MSFEELDQLYRSSPRPAAIADLNGHAIGAMLAWRSPRTGPLASL
                     LRTLGNSTVFPWEGKSFHTKGTESGGGINRINLLGKRQWFPFRTRFDASFLDGKPTFV
                     LDYSGPGNPPLIRQIVDEVREVAPRLYMGPAALKVAGRPRPILFFAVSLQ"
BASE COUNT          953 a         1142 c         1222 g          974 t
ORIGIN      
        1 ttagtttcag ctttcccttt agttctccag ccgccgcagc caacagcact ggctgcatcg
       61 cgccagccgg tgcgtgcgcg ccacggcatc gtcgcagcta cgaatcagct cggcgagcgc
      121 gtcggcgtgc aaattctgca aagcggcgga aatgcgatcg acgctgccat cggcgtagcc
      181 tttgcgttgg cagtaaccca tccggccgca ggcaatcttg gtggcggcgg cttcatgatg
      241 attcgtttga gtaacggcaa aactaccgca atcgattatc gggaaatggc gccggctgcg
      301 gcccatcgaa atgtttatct cgataaggat gggaatctaa tcaagggtga aggctcttct
      361 acggttggat atcgcgcgtc gggagttccc ggcaccgttg ccggtttgga gctggcattg
      421 aagaagtatg gctcaggcaa gttaacctgg gcgcaactgg tggagccggc gcggcgcctg
      481 gcgagcgaag gatttccgat gggctacggt ctcgcgcgct cgctcaaccg caacgagtat
      541 ctggccaagt atgaggagac gcggcgcata ttcttacgca atggaaacta ctatcaggag
      601 gatgagatat tccgccagcc agaactcgca gcaacactca cgcgcttgca acgctcagga
      661 ccgaaagagt tttaccaggg gcgcacagcg caactcatcg ccgctgacat gaagcggaac
      721 aatggcctaa tcacaatggc agacatgcgc gggtatgttg ccaaggagcg gcagccactg
      781 cagggtagct atcgtggtta tccagtgatt tccatgccac ccccgtcgtc aggcggcgca
      841 gtactcttgg agatgctgaa catccttgaa ggcttcgaac tggacaaata cgactgggcc
      901 tcgtccgaac gctaccatct tacgactgaa gcgatgcggc gcgcgtttgc cgatcgcgcc
      961 gaatacatgg gcgacgccga ttttgtaaaa gttccggtgg cgggtttgat agataagtcg
     1021 tatgccgtca aactgcgcca gtcgatcaat tttgaacgtg cttcctcaag caccgaagta
     1081 cgagcgggtc agccaatggg ttatgaatct gaagaaacaa ctcacttcac cgttgttgat
     1141 gtgcaaggca acgcggtggc aaatacctat accttaaatg actcgtttgg ctccggcgtg
     1201 gtagcgaaag gtaccggagt tttcatgaac aatgagatgg acgattttgc cgccaagcca
     1261 ggcacaccca atttgtacgg attaatccaa ggcgagcgta acgcggtggc ggcaggaaag
     1321 cggccgctgt cagcgatgac gccgacgttt gtcctgagga aggacggcag tttgtggttc
     1381 acaaccggca gtcccggcgg accgacgatc atcaatactg tcctgctggt gattacgaac
     1441 gtcatcgact acaaaatgaa catccagcaa gcgattgatg cgccgcgcat tcatcatcag
     1501 tggttgcctg atcaactcgt cttcgaacca tacggattgt ctggagatac gcagaaggcc
     1561 ctactggctc gcggacataa gtttgcaaag ccaacttacc tcggcaatgc ggaaggaatc
     1621 atgatcgaag aaaagaccgg agtgcgtttg ggcgccagtg acccacgtcg cagtgacggg
     1681 caggctgtcg gttattgaac acgaatctga ttttcacgtc ttcagagtgt ggaaaaccaa
     1741 ttatggcttt cattctcacc gcgcttcagc gcggtgttaa gtgagaagag aaggaaaccg
     1801 cttaaacggt ttcgttgagc attgtgactc ctataacacc ggtgagaatg aaagctttta
     1861 atgggctgga gcccggcgag aacggaaccc tgtctgcaaa catggccgcg cccaattaac
     1921 caaattgtca ttaccagatg tcctagaaac cgaagaacaa aagcttcttg catgtgtgca
     1981 ctgcggcctt tgtctcgaag cctgccctac ctacacgctt actggcgacg aaaacgatag
     2041 tccgcgcggt cgcatttacc tgatgcgtgc ggttgcggaa gaaagacttc gctctgactc
     2101 cgttacgttt aaaactcaca tcgatcgctg cctgggatgt cgcgcgtgtg aacctgtttg
     2161 cccggctggt gttgagtatg ggcaattgct cgaagcgagc cgcgcggcaa tcttcactcc
     2221 ggaagtgccg aaggggttta gatatcgcct gctgcggttt gcgctgcggc atgtttggct
     2281 gcagcctcgg cgcttacgat tctcttttgc gttagcgcgc ctgctgcgcg atacgggttt
     2341 ggcaaagttg cttttgcagt ctcgagttat cggcttgttt tccaagcgtg gtgagtttgg
     2401 cctcgccttg ctcctgagtt ccgcacctca gatcgacacc agtgctggta agggactttc
     2461 ggaacctgct gcggacgagc aaacgcagac cgccctgtta ttcaccggct gcgttaccgc
     2521 tggacttttt gggcgagtaa atgaagcaac aaagcgagta ctgctggtta atggcgtgca
     2581 agcagaagca gcgccgcacc aggtatgttg cggcgcactg cacgcgcacg caggtgattt
     2641 ggagggagca cgcttgctcg cgaggcaaaa catttcagca tttggcgctg gtgacacacc
     2701 ggtaatcacg aatgccggcg gctgtggagc gatgctggtt tcctacggtc acttgcttgc
     2761 cgacgactca acttttgccg agcgcgcgag aacgttctcg gcgcgagtgc gcgatgtcag
     2821 tcaacaactc gaaacgaaaa cgattgtgaa cggtgcccat ttggatggcg ctgtcacgta
     2881 cgacgcgtct tgtcacttgc tgaatggaca gcatgcggcc gaggcgccct tgaaagtcct
     2941 gcgggcaatt cccgatctta atttcgtacc acttgaaggt gcggagcgct gttgcggcgg
     3001 cgcgggagtt tacaacctgt tggagccgga gatgtctcag ggcgtcttag cggaaaagct
     3061 cgctcatgtt cgcgagactg gcgcgaccgt tctggccacg ggcaaccccg gatgtcagat
     3121 gcagatcggc gcggggttgc aggtaaaggg aatggaattg cgaacctgcc atccggttga
     3181 actcctggac atgtcgtacc ggcaggctgg gtactactca gaggattcct aaacatgtcg
     3241 cagagaagcg caaaccacat caccctcgct atttttgcgc ccttactcat tgtggttggc
     3301 gtggctggat tcctggtacc ggcgcacctc agtctcacca gcggcgcgcc ggcttacaac
     3361 gtttttcata tctgcttcgg attgctcggc ctgttggtac tctgggcgcg aaaagaacgg
     3421 tggataagtt tgttcaatgc cggattcggc ctcatcgatc tgtttcaggc gttggcgagc
     3481 tacactgatt tgacgcctaa acagtatttt ctttggaccc gcgtcgatga tattttgcac
     3541 gtcgtgatcg gattaacgct tcttattatc ggctgctacg gactagtgag agaaaagaag
     3601 ctgtgaaagc tccggcttta ccgccggagg tgaggcggcg aagccgaacc taacctgacg
     3661 cgcaggaaat cgcagacgta ccctcaacaa aaggagacca aaccaaatgg tggcactctc
     3721 acgtgaagtc tctaacatgc aatccttaaa tcttgaggtc ctccggcaaa tgagcttcga
     3781 agaattagat cagctctatc gttcgtcacc gcgacccgct gcaatcgcgg atctcaatgg
     3841 ccacgcaata ggcgccatgc tggcctggcg ttcgcctcgc actggcccgc tggcgtccct
     3901 gctgcgtact cttggtaatt caacagtctt tccctgggag ggaaaatcgt ttcacaccaa
     3961 gggaacagaa tcaggtggcg gtatcaaccg gataaacttg ctgggcaaaa gacaatggtt
     4021 tccgtttcgc acacgcttcg acgcttcgtt cctcgacggc aagccaactt ttgttttgga
     4081 ctactccggt cccggcaacc cgccattgat tcggcaaatc gtggatgaag tacgagaagt
     4141 agcgccgcgg ctgtacatgg gcccggccgc gttgaaagtc gctggccgtc cccggcccat
     4201 cctatttttt gctgtgtcac tgcagtaacg gaaatgtctg agtttcgcat tttgactctc
     4261 ccgctttgat tgcccactcg agcatcaatg a
//
LOCUS       DASUEX010000554         2289 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1598385, whole genome shotgun sequence.
ACCESSION   DASUEX010000554 DASUEX010000000
VERSION     DASUEX010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1598385"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1152
                     /gene="gndA"
                     /locus_tag="VFV61_09270"
     CDS             <1..1152
                     /gene="gndA"
                     /locus_tag="VFV61_09270"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:NF006765.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HEX4900710.1"
                     /translation="DNGDIIIDGGNSHFNDTERREQHLKSEGYFMIGSGTSGGEKGAL
                     LGPSLMPGGDRDAYEKIRPSWEAIAAKVPDGSCVTYIGPGGSGHFVKMVHNGIEYGDM
                     QLIAEAYGVLRNGFGLGVDQVAEIFEEWNKGDLESYLIEITGKILRVKDPETGQPLVD
                     LVLDKAGQKGTGKWTSQLALDLGVAIPTIDAAIVARTLSSQKDARVLASGKLRGITNG
                     DPGKYGPEVKSQIHDALYASKICSYAQGMNLIKAGSDEFGWSINLSEVARIWQGGCII
                     RAKFLGRIKEAYLRNPQLGSLLLDEEFGHSVEQMQPGWRAIVAEGMQRGLPVLAMSAS
                     LGYFDMYRSAQLPLNLTQAQRDFFGSHTYERVDKPELGFVHTEWEGIAD"
     gene            1318..2046
                     /gene="pgl"
                     /locus_tag="VFV61_09275"
     CDS             1318..2046
                     /gene="pgl"
                     /locus_tag="VFV61_09275"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEX4900711.1"
                     /translation="MPNVQVCETPEEVARTAAEFIVKLAKPATHNHQFSVALSGGNTP
                     RRVYELLASSEFKNQINWQFVHLFFGDERPVRADHPASNYGMALGNLISRVPIPPGNV
                     HPISGEGTPQESAKSYQHELKSYFGNSPWPRFDLVLLGMGEDGHTASLFPRTKALSEE
                     SAWVAANWVEQRQEFRITLTAPAINSAAQVIFLVTGENKAARLAEVLNGPFQPDLLPA
                     QLIKPDQGELTWIVDAAAASQFRV"
BASE COUNT          527 a          567 c          673 g          522 t
ORIGIN      
        1 gataacggcg acatcatcat cgacggcggc aattcacact tcaacgatac ggagcgacgt
       61 gagcagcatt tgaagagcga aggttatttc atgatcggtt ccggaacgtc cggcggtgaa
      121 aaaggtgcgt tgctggggcc aagtctgatg ccgggcggcg atcgcgatgc ttatgaaaag
      181 attcgtccga gttgggaagc gattgcggca aaagtccccg atggttcttg cgtgacatac
      241 atcggaccgg gtggctcagg tcatttcgtg aagatggttc acaacggcat cgagtacggc
      301 gacatgcagt tgattgccga agcgtatggc gtgctgcgga acggattcgg actgggcgtg
      361 gatcaggtag cggagatttt cgaggagtgg aacaaaggcg acctcgaatc ctatcttatc
      421 gagatcactg gaaagatttt gcgcgtcaaa gatcccgaga ccggtcagcc cctggttgat
      481 ttggtgctgg acaaggctgg tcagaagggt acgggaaagt ggaccagcca gttggccctg
      541 gatctcggcg tggcgattcc caccattgat gccgcgatcg tggcacgcac tctgtcgtca
      601 caaaaggacg cccgagtgtt ggcgagtggc aagctacgcg gcatcacaaa tggtgatccg
      661 ggcaagtatg gtccggaagt taagagtcag attcacgacg cgctttacgc ttcgaagatt
      721 tgttcgtacg cgcaaggtat gaatttgatc aaggccggct ccgatgaatt tggctggagc
      781 atcaatctca gtgaagtggc gcgcatctgg cagggtggct gcatcattcg cgcgaagttt
      841 ctgggtcgca tcaaggaagc ctatttacgg aaccctcagc ttgggagtct gctcctggat
      901 gaggagttcg gtcatagcgt agaacaaatg cagccgggct ggcgtgcgat cgtggcggag
      961 ggaatgcagc ggggtttgcc ggtgctggca atgagcgcga gccttggtta tttcgatatg
     1021 tatcgcagtg cccagctacc gctgaatttg acccaggcgc agcgcgactt ttttggttcg
     1081 catacgtatg aacgcgtgga taagccagaa ctggggtttg ttcatacgga atgggaaggg
     1141 attgccgatt gatggttgcc gattgccgat tgccgattga ctattggagt ctttcgaaaa
     1201 tgactggttc tactaagact cggatttgtc gccaactcgc taattggaaa ttggcaatcg
     1261 caaatcggca atcaaacgtg ctggctcctg gctcctgact tctatctcct gtccctcatg
     1321 cctaacgttc aagtctgcga aactccggaa gaagtagccc gcacggcggc ggaattcatc
     1381 gtcaaacttg ccaagcccgc tacgcacaac caccagttta gtgtcgcgtt gtcgggcgga
     1441 aacacaccgc ggcgcgttta tgaacttctg gcaagtagcg aattcaaaaa ccagatcaac
     1501 tggcagttcg ttcacctttt ctttggtgat gaacgacccg ttcgtgcgga ccatcccgcg
     1561 agcaattacg gcatggcttt gggtaacttg atttctcgcg tcccgattcc cccaggcaac
     1621 gtgcatccga tttcaggaga gggaactccc caggaaagcg ccaagagtta tcagcacgaa
     1681 ctaaagtctt acttcggcaa ttcaccctgg ccgcgatttg atttggtgct actcgggatg
     1741 ggagaggatg ggcatacggc ttcacttttc ccgaggacca aagcgctttc ggaagagagt
     1801 gcctgggtcg ctgctaattg ggtggagcaa cggcaggagt ttcgcatcac gctgacggcg
     1861 ccggccatca actcggcggc gcaagtcatc tttttggtga caggcgagaa caaagcagcg
     1921 cgcctggcag aagtgttgaa tggacctttt cagccagacc tcttgccggc ccagctcatc
     1981 aaaccggatc aaggtgagtt gacctggata gttgatgcgg cggcggcttc gcaatttcga
     2041 gtctagagtt cagcgtttcg aacacatcca gctgcaccga ttaaacagag acgcaaagac
     2101 gcggagacgc agagacgaca aggaaagaga aaagcagttg aggcgtcgac ctcactgtct
     2161 aaatcttctt ttctttttct ttgtgtctct gcgtctccgc gtctctgcgt ctctgcgtct
     2221 tctgcgacgc gaaacccgaa actcgtctag cagctcacat agcccaggag ccagaacact
     2281 agaatgaaa
//
LOCUS       DASUEX010000555         4877 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_909392, whole genome shotgun sequence.
ACCESSION   DASUEX010000555 DASUEX010000000
VERSION     DASUEX010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4877
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_909392"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..780)
                     /locus_tag="VFV61_09280"
     CDS             complement(<1..780)
                     /locus_tag="VFV61_09280"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:NF015022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HEX4900712.1"
                     /translation="MRALIAAAGTGGHIYPGIAVAKEILRRDEKSQIRFVGTARGLEN
                     KLVRQAGFELSIIDSAGLKSVGATARLRGLAILPRSFAGARRIIREFRPDVVIGAGGY
                     VSGPVLLTAALMNLPTMVLESNALPGWTNRKLARFVDKAAVTFEASLPFFRGKGVVTG
                     NPVRREFFEIPVRQREAGQFSLLIFGGSQGAHAINEAMVAALPELEAQRGKLRLTHQT
                     GEADYERVRNAYLDAGWDEQAEVKRYIDDMVKSFAAADLVIC"
     gene            complement(783..1982)
                     /gene="ftsW"
                     /locus_tag="VFV61_09285"
     CDS             complement(783..1982)
                     /gene="ftsW"
                     /locus_tag="VFV61_09285"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HEX4900713.1"
                     /translation="MAKKLSPDKWLFAATVGLALFGVVMVYSASAVLAQNEGHSQFFY
                     VIKQGIWTLIGFVVMFAAMQLDYRHLKNRRVVYGLLVVTTLMLLAVFAFPKVNGAHRW
                     IKVSGFSIQPSELSKLTLAVFLAYFLERRAGEEARFWQTFVPCGVITAALAFLIVLEP
                     DLGTAMMLAVLFVVVTFTAGARLLHLGMAAAPALVGVIGLLILVPWRLQRMVTFLDPW
                     ADPQNSGFQVVQSLLAIGSGGPNGLGFAQGKQKMLFLPFAHSDFIFAVIAEELGLVGA
                     LAVIAVFCLILGRGLRASLLAPDRFGMLLSLGLVTVIVTQALFNISVVLSLLPTKGIP
                     LPFVSYGGSSLVPTLAAMGILLNISQQAVGRLNFGALRSSTARDEAAPSRVRMATARR
                     LASSRSG"
     gene            complement(2062..3444)
                     /gene="murD"
                     /locus_tag="VFV61_09290"
     CDS             complement(2062..3444)
                     /gene="murD"
                     /locus_tag="VFV61_09290"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEX4900714.1"
                     /translation="MKVAGKKVLIIGAARSGIGCARFLAARGATVALNDQKPFEKWSS
                     EAQALKAEGIGCISGDVPGWLLDNIELVVVSPGVPTKSIPIRYAERAGAEVIGEVELA
                     ARYLKGRIVGITGSNGKTTTTALIGELLKDAGIKVQVGGNIGTPLISLVESSIVDGWT
                     VVELSSFQLETIKEFHPRVAVVLNVTPNHMDRYESLTDYAGAKHRIFMNQTPSDVAVL
                     NADDEIVSSWAKGLRAHVVEFSVAGELAEGLFLRGDELISRTAAGEKVLLRRQEMKLR
                     GQHNVENALAALAAGLACGAGPDSMRGTVSRFQPVEHRLEFVRELGDVTFYNDSKATS
                     VDATVKALEAFASEAGKIVLIIGGKGKQAPYAPLAPLVKAQARELILIGEDAETIARE
                     LGAYAAHQRATDMQDAVKRAFATAQPGDIVLLAPACASFDMFESFEHRGRVFKEHVQS
                     LTSKVQGPSS"
     gene            complement(3587..4741)
                     /gene="mraY"
                     /locus_tag="VFV61_09295"
     CDS             complement(3587..4741)
                     /gene="mraY"
                     /locus_tag="VFV61_09295"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEX4900715.1"
                     /translation="MIYYFLYELLYGHFKEKDWFLIKGLNVFQYVTFRTAYASITALL
                     LSLLFGGALIRALKGWNIGQQIREEGPQGHMAKRGTPTMGGVLIVGSVLISTLLWARL
                     SNLYIWTAIVATVLFAAIGFMDDYAKMAKQRSLGLTGRQKLLFQFLIALGVWTVLFIS
                     TKWLASDYSWNVSIPFLKATAEPYGVSYIGPYLYLVLILVVLIGWSNAVNLTDGLDGL
                     AISVTFIAMTALTGFTYLSSDLRWARYLELTHRPEAAELTVFCGAMAGASLGFLWYNA
                     PPAEVFMGDVGSLAIGGAIGTVSILTKQEFMLVMVGGVFVMEAMSVMLQVGVFKMTKR
                     TTGTGRRLFKMTPLHHHFEMQDWKEPKIVFRFLILAILFALLSLSTLKLR"
BASE COUNT         1157 a         1531 c         1175 g         1014 t
ORIGIN      
        1 acaaattacg agatccgccg cggcgaacga tttgaccatg tcatcgatgt aacgtttcac
       61 ctcagcttgc tcatcccaac ccgcgtcaag atatgcattg cgcactcgct cataatcggc
      121 ttctccggtc tggtgagtga ggcgcaactt cccccgttgt gcttccagtt caggcaaggc
      181 ggccaccatg gcttcgttga ttgcgtgcgc gccctgcgag ccgccaaaaa tcaaaagcga
      241 aaactgcccc gcctcacgtt gcctgactgg tatttcaaag aactcgcgcc gcactggatt
      301 tcccgtcacc acgcccttgc cacgaaaaaa tggtagtgac gcctcgaacg ttactgcggc
      361 cttgtcgaca aagcgggcta actttcggtt cgtccagccc ggcaaagcat tagattccaa
      421 aaccatcgtc ggcaagttca tcagcgccgc cgtcaacaac accggacccg agacgtaacc
      481 gccggcccca ataactacgt ccggccgaaa ctctcgaatg atgcggcgcg cgccagcaaa
      541 gctccgtggc agtatcgcca aaccgcgcaa ccgtgccgtc gctcccacac tcttgagtcc
      601 ggcactgtca atgatcgaaa gttcaaaacc cgcttgtcgc accaatttgt tctcgaggcc
      661 gcgcgccgta cccacaaacc gaatctgcga cttctcatcc cggcgcagga tctcttttgc
      721 aaccgcgatt cccggataga tgtggccgcc ggtcccggca gcggcaatca atgcgcgcat
      781 gtttatccag atcgcgacga tgccaaacgc ctcgccgttg ccattcgtac ccgggacggg
      841 gcggcttcat ctctcgcagt cgaggatctt agcgcgccaa aattcagtct tccgaccgcc
      901 tgttgcgaga tattcaaaag aattcccatg gctgctagcg tgggcaccag cgacgaaccg
      961 ccatatgaga caaacggcaa cgggattccc ttcgttggca gcagtgagag aacgacgctg
     1021 atgttgaaaa gagcttgcgt cacgatcacg gttaccagtc ccagcgaaag cagcatccca
     1081 aagcgatccg gcgctaacag cgacgcgcgc aaaccccgcc cgaggatcaa acagaacacg
     1141 gcaatcaccg ccagcgcgcc tacgagtcct aactcctctg cgatcaccgc gaaaatgaaa
     1201 tccgaatgag caaagggcaa aaacaacatc ttctgtttac cctgcgcgaa ccccagacca
     1261 ttcggcccgc ccgaaccaat ggccaacaac gattgcacca cctggaaccc gctgttttgc
     1321 ggatcggccc agggatcaag aaatgtaacc atgcgttgca gtcgccaggg caccaggatt
     1381 agcagtccaa tcacgccgac taatgccggt gccgccgcca tccccaggtg cagtaagcga
     1441 gcacccgcgg taaatgtgac tactacaaag aggaccgcca gcatcatcgc agttcccaaa
     1501 tcaggttcca gaacaatcag gaaagcgagg gccgcggtga tgactccgca gggaacaaac
     1561 gtttgccaga accgagcttc ttcacccgca cggcgctcca gaaagtaagc gaggaatacc
     1621 gcgagggtta gtttcgaaag ctcggaaggc tgaatcgaaa atccgctaac cttgatccaa
     1681 cgatgagcgc cgtttacctt gggaaaggcg aacacggcca gcaacatcaa cgtcgtgacc
     1741 accagcaggc cataaaccac gcgccggttt ttaaggtgcc tgtagtcaag ctgcatggct
     1801 gcaaacatca caacgaaccc gatcaaggtc cagatgcctt gtttgattac gtagaaaaac
     1861 tggctgtgcc cctcgttctg cgccaggact gctgaggcgc tatacaccat aactacgccg
     1921 aacagcgcca gtccgactgt cgccgcgaac aaccacttat ctggacttag cttctttgcc
     1981 atttaatctc tatgtccaaa gtccaatggt gaagtccgaa atccgacgtc gatagacatc
     2041 tcacgtcttc aaagctcaac atcagctaga agggccttgg actttggacg tgagactctg
     2101 gacgtgttct ttgaacactc ttccccggtg ctcgaaactc tcaaacatgt cgaagctggc
     2161 gcacgctggt gctaacaaca cgatgtcgcc gggttgtgcc gttgcgaagg cgcgcttaac
     2221 ggcatcttgc atgtcggtcg cgcgctggtg cgctgcatac gcgcctaact ccctggcgat
     2281 cgtctccgcg tcctcaccga ttagtattag ttcgcgcgcc tgcgctttca cgagcggtgc
     2341 taacggcgcg taaggcgcct gctttccctt cccgccgata atcaagacta tttttcccgc
     2401 ttcagatgca aacgcctcca gcgctttcac tgtcgcatca acacttgttg ctttggagtc
     2461 gttgtaaaac gtcacgtcgc caagctcccg cacaaactcg aggcgatgct caaccggttg
     2521 aaagcgactg acagttccgc gcatcgaatc cggaccagcc ccgcaggcta atcccgcagc
     2581 cagcgccgcc agtgcgttct ccacattgtg ttgcccacgc aacttcatct cctgacgtcg
     2641 caaaagcacc ttctcgccgg cggccgttcg cgaaatcagt tcatcgccac gcaggaagag
     2701 tccttccgca agttcacccg caacgctgaa ttccaccacg tgagcccgca gtcccttcgc
     2761 ccacgaggaa acaatctcat cgtcggcatt caacactgcg acgtcggacg gcgtttgatt
     2821 catgaaaatc cgatgcttcg ctcccgcgta atcggtcagg gattcgtaac gatccatatg
     2881 attcggtgta acgttcaaga ccactgccac cctcggatga aactccttga ttgtctcaag
     2941 ttgaaaactc gagagttcta ctactgtcca tccgtcaact atcgagcttt ccacgagtga
     3001 gatcagcggc gtcccgatgt tgccgccaac ctgcaccttt atgcccgcat ccttcagtaa
     3061 ctccccaatc aaagcggtgg tcgttgtctt tccgtttgat cccgtgatgc caacgatccg
     3121 acctttcaaa taccgcgctg ccaattcgac ttcgccaatc acctctgccc cagctcgctc
     3181 ggcatatctg atcgggatgc tcttagtcgg aactcccgga ctgacaacta ccagttcaat
     3241 gttgtcgagc aaccaacccg gaacatctcc ggagatgcaa ccaatgccct ctgcttttag
     3301 cgcctgggct tccgaactcc atttttcaaa cggcttctga tcgtttaggg cgaccgtcgc
     3361 gcctcgcgcg gcgagaaacc ttgcgcagcc aatgccactg cgcgcggcgc caataatcag
     3421 tactttcttg ccggcaactt tcaaagggtt tcgggtgtcg actgccgggt gtcggtaatt
     3481 tcgtcagcgg ttccaatttt catctgctct tgccgctgtt ctttccgtaa ttcgtggacc
     3541 gaaagaaggg acacacggca cctgataccc cgcaccccgt caccctttac cggagtttca
     3601 aagtcgagag actcaacaac gcgaaaagta ttgccaggat caaaaagcgg aagacgatct
     3661 tcggctcctt ccaatcctgc atttcaaagt gatggtgcaa gggcgtcatc ttgaagagtc
     3721 ttctgccggt cccggtggtg cgcttcgtca tcttgaagac gccaacttgc aacatcaccg
     3781 acatggcttc cattacgaag actccgccca ccatcaccaa catgaattcc tgtttcgtca
     3841 aaatgctgac cgtcccgatc gccccgccaa tcgccaaact gccaacgtcg cccatgaaaa
     3901 cttccgctgg cggtgcgttg taccaaagga aaccaagact ggctcccgcc atcgcgccgc
     3961 agaagactgt caactccgcc gcttccggtc gatgcgtcaa ttccaaatag cgtgcccagc
     4021 gcaagtcgct gctcagataa gtaaagccgg tcagcgcggt catcgcgatg aaggtgacgc
     4081 taatcgccag accatcgagc ccatccgtca ggttcaccgc gttagaccag ccaatcaaca
     4141 cgaccaggat caagaccagg taaagatacg ggccgatgta gctgacgccg taaggctctg
     4201 ccgtcgcttt caagaacgga atactgacat tccaggagta gtcgctggcc agccacttcg
     4261 tcgaaataaa aagaaccgtc caaactccca gtgcgatcaa gaattgaaac agcaacttct
     4321 gcctgccggt tagtcccaga cttctctgct ttgccatctt cgcgtagtcg tccatgaacc
     4381 caatggcggc gaaaagcacg gttgcgacga tggccgtcca aatgtaaagg ttcgacaggc
     4441 gcgcccacag cagggtcgaa atcaaaaccg aaccgacaat cagcacccct cccatggtcg
     4501 gcgtcccgcg cttggccatg tgcccttgcg gaccttcttc gcgaatctgt tgtccaatgt
     4561 tccaaccctt gagcgcccgg atcagcgcgc caccaaacag tagcgaaagt agtagtgctg
     4621 tgatcgaggc gtaggcggtg cgaaaagtga cgtactgaaa aacgttaagt cccttgatca
     4681 gaaaccagtc cttctctttg aagtggccgt aaagcagctc gtataagaag tagtagatca
     4741 tctaagcagt agtcggtagt cagtagtcgg tgccttgcgg cctacctgcc acccccgact
     4801 tcttctggaa aagctttctt aatcgcgctg acaaccttat ccgttgcgac acctctcgaa
     4861 cctttaatca acacgag
//
LOCUS       DASUEX010000556         2018 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_529249, whole genome shotgun sequence.
ACCESSION   DASUEX010000556 DASUEX010000000
VERSION     DASUEX010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_529249"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="VFV61_09300"
     CDS             <1..268
                     /locus_tag="VFV61_09300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEX4900716.1"
                     /translation="VHSVLDMLGKPRSLIQFVKDRLGHDRRYAIDPSMIESEVGWRPR
                     ETWESGLRKTIEWYRDNTPWIEHVRSGAYREYYARQYGTEASAS"
     gene            265..1155
                     /gene="rfbD"
                     /locus_tag="VFV61_09305"
     CDS             265..1155
                     /gene="rfbD"
                     /locus_tag="VFV61_09305"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:TIGR01214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEX4900717.1"
                     /translation="MKVAVTGAGGLLGRAVKTHCAAHSDEVHAFDHRALDITNAAAVK
                     SQLTALKPEVVFNCAAWTDVDGCESDPSRAESANALGPQLLASTCREIGALLITISTD
                     YVFDGAKDGFYTQRDHPNPISVYGRFKFEGERRAQTAYARTIVVRSGYIFGAEGTNFL
                     STVLDRARRGARLKAISDMTGTPTYAPDLAARLRELAEFDLPGIYHVVNAGDGVSFET
                     FARTALKLGGVDPGLLQSTTLAELERPAPRPRNSRLRCLLSEAIGLPPLRNWQAALED
                     FVTSQSGTDKPPTAAASPTG"
     gene            1283..>2018
                     /locus_tag="VFV61_09310"
     CDS             1283..>2018
                     /locus_tag="VFV61_09310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900718.1"
                     /translation="MTQDERLLPVRPGRDAETSLAEIAQGRAYGKYSRYGNQAETNVR
                     EYLHIILKRKWLILSLVLVITSLVTIQAYREPSIYQGSTTIRIEPKQGSILQTGGALL
                     INQTDPNFWGTQLKLLQSPVLARQVVLTLQLDKNPEFLGGQANSGIFSSLKRILGRDG
                     TKLASAEGQNADPDPVGEREMQERQLTTEQLAALEPYEDAVVGGQIITPVEKTNLVVI
                     SFTHTNPQLAKQISDTLAEVFVQNNVE"
BASE COUNT          451 a          587 c          585 g          395 t
ORIGIN      
        1 agtgcacagc gtgttggaca tgctcggcaa accacgctcg ctcattcagt ttgtaaagga
       61 tcgtctgggt cacgatcggc gttacgccat cgatccatcc atgatcgaaa gtgaggttgg
      121 ctggcgtccg cgcgagacat gggagagcgg cctgcgcaaa acgatcgagt ggtatcgcga
      181 caacacgccg tggattgaac acgtgcgaag cggcgcgtac cgcgagtact acgcgcgcca
      241 gtatggaacg gaggcgtctg caagttgaaa gtcgccgtca cgggtgcagg aggccttctg
      301 ggccgcgcag tcaagacaca ttgcgccgca cacagcgatg aggtgcacgc gttcgatcac
      361 cgcgcgttgg acatcaccaa cgcagcggcg gtgaagagtc agctcaccgc cctcaagcct
      421 gaggttgtct ttaactgcgc ggcgtggacc gacgttgatg gatgcgaatc tgatcccagc
      481 cgcgcagaga gcgcgaatgc gctcggtcct caactgctcg cttccacctg tcgtgaaatt
      541 ggcgcgctgt tgatcacgat ctcaactgat tacgttttcg atggggccaa ggacggcttt
      601 tacactcagc gcgatcaccc taatccgatc agcgtgtatg gccgcttcaa attcgaaggc
      661 gaacgtcgcg ctcagactgc ttacgcacgc accatcgtcg ttcgcagcgg atatattttc
      721 ggagcggagg gaaccaactt tctcagtacg gtcttagatc gcgctcggcg cggcgcacgt
      781 ttgaaagcga tttcagacat gaccggcacg ccgacttacg cgccagacct cgccgcgcgg
      841 cttcgtgaac tcgctgagtt cgacttgccg gggatctatc acgtcgtaaa cgccggcgat
      901 ggcgtcagct ttgagacgtt tgcacggact gcgttgaagc tgggtggtgt cgatcctggt
      961 ctgctgcaat caaccacgct ggcggaactc gagcgcccgg cgccgcggcc ccgcaactca
     1021 cgattgcgat gtttgctgtc cgaagccatc gggctgccgc cattgcggaa ctggcaagca
     1081 gccctggaag attttgttac atcacaatcg gggaccgaca aaccgccgac ggccgccgcc
     1141 tcgccgacag ggtaacgcct ttaaaagcgg actagcgcag gtggcgcatg ccgccctttt
     1201 cggcttgcct cgcggaaatg gtctgttatt atgatcgtcc agttcccgag gccaaaagct
     1261 taaagaagta ggatttagca ctatgaccca agacgaacgc ctgctcccgg tccggccggg
     1321 gcgcgatgca gaaacctcac tcgcggagat tgcccagggc cgcgcttatg gaaagtacag
     1381 ccggtacggc aatcaggccg agacaaacgt ccgcgaatac cttcacatca tcctcaagcg
     1441 gaagtggctc attttgagtc tggtgctcgt gatcacttcg ctggtgacga tacaggcgta
     1501 tcgcgagcct tcgatctatc agggcagtac gacgattcgc atcgagccaa agcaagggag
     1561 catcctccaa accggcggcg cattgctcat taaccaaacc gatccgaact tctggggcac
     1621 acaactgaag ctgttgcaga gcccggtgct cgcacgccag gtggtactca cgcttcagct
     1681 ggacaaaaac ccggagttct tgggtggcca agcgaacagc ggaatatttt cctcgcttaa
     1741 gagaatcctc ggacgcgacg gaaccaaact cgcgtcggct gaaggtcaaa atgccgaccc
     1801 tgatcctgtt ggtgagcgcg aaatgcagga acggcaactc accaccgagc aactcgcagc
     1861 gcttgagcca tacgaagacg cagtggtggg cggccagatt atcacgccgg tcgagaagac
     1921 gaacctggtc gtgattagtt ttactcacac gaatccacag cttgccaaac aaatttccga
     1981 cacgctcgcc gaagtatttg tgcagaacaa tgttgagc
//
LOCUS       DASUEX010000557         3625 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_433365, whole genome shotgun sequence.
ACCESSION   DASUEX010000557 DASUEX010000000
VERSION     DASUEX010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3625
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_433365"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="VFV61_09315"
     CDS             <1..510
                     /locus_tag="VFV61_09315"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX4900719.1"
                     /translation="NSSLSEFTCRHMPVAADVKLGADVKIFQPELVNLYGCTIGRETK
                     IGAFVEIQKNATVGARCKISSHSFICEGVTIDEEVFVGHGVMFTNDRYPRAVNEDGSL
                     QTENDWVVVPTRVRRGAAIGSNVTVVAGVVIGERALVGAGAVVTRDVPDYAIVAGVPA
                     RVIGDVRRK"
     gene            664..1674
                     /locus_tag="VFV61_09320"
     CDS             664..1674
                     /locus_tag="VFV61_09320"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX4900720.1"
                     /translation="MIKIGVIGYGYWGPNLVRNFMEAPGSTVVAVCDLRPERLEQLTN
                     RHPSIKSCTNSEALLNDSQIEAIAIATPVSTHFDLALAALRAGKHVLVEKPLAANSEQ
                     ALQLIAEAAACKRVLMVDHTFVYTGAVRKIRELITENHLGEIYYYDAVRVNLGLFQHD
                     VNVIWDLAIHDLSIMDYVLPSRAVAVSATGISNIPGQPENVAYITLFFGNPQIAHVHV
                     NWLTPVKVRHTLIGGSDKMILYDDLEPSEKVKVYDKGVRVSQSPEAVYEMLVSYRSGD
                     MWAPRLDATEALHTEALHFIDCIQNNKPPVTDGHAGLRLVRIVEAAERSLRARGELVE
                     IQ"
     gene            1730..2863
                     /locus_tag="VFV61_09325"
     CDS             1730..2863
                     /locus_tag="VFV61_09325"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEX4900721.1"
                     /translation="MIPFVDLKAQYLTIKDEIDTAIRSVVESCHFVLGEQVEAFETDF
                     ARFCATEYALGVNSGTSALHLALLAAGVRAGDEVITVSNTFVATAAAICYTGARPVLV
                     EMEPRSCTMDPAKIEAAITPRTKVIMPVHLYGGCADMDPIREIASRRNLIVIEDAAQA
                     HGAEYKDRRAGSMSDIACFSFYPGKNLGAYGEGGAVVTDNSQYAATIKRLRDHGQGEK
                     YYHDEVGYNYRMEGIQGAVLRVKLAHLDDWTAARRQHAATYRQSLAGSGMRLLEPYGN
                     SAWHIFPVFTQRRDELIEYLKSRGVATGIHYPIPVHLQRGYADLGYKPGELPLTEQAC
                     REVLSLPMYAELPNEDLLEVVAAIHEFAANTSQPTAEATRAHS"
     gene            2876..>3625
                     /locus_tag="VFV61_09330"
     CDS             2876..>3625
                     /locus_tag="VFV61_09330"
                     /inference="COORDINATES: protein motif:HMM:NF015940.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF354 domain-containing protein"
                     /protein_id="HEX4900722.1"
                     /translation="MRIWIDLANSPHVPFFRALISEFVARGHQFEVTAREFAETVPLA
                     RSAGLEPTVIGVHGGRELTAKAGNLFQRAWALRAWASGRAFDLAVSHNSYSQILAARG
                     LGLRCVTLMDYEHQPANHLAFRLASTVIVPRAFPEEDLSRYGAVAIKVKRYNGIKEDV
                     YLADFESDPAFAGKLREIGAKLESTIVVVRPPARDALYHRFENDLFEGLLKRLSETPQ
                     VTTILLPRNAGQRTTYAARVGPRTIMPEEPLD"
BASE COUNT          815 a          962 c         1031 g          817 t
ORIGIN      
        1 aacagcagtc tcagtgaatt cacttgtcga catatgccag tcgccgctga cgtaaaactc
       61 ggtgcagacg tcaagatctt tcagcctgag ctagtcaatc tttatggctg cacgatcggt
      121 cgcgagacga agatcggcgc ctttgttgag atacaaaaga atgcgactgt cggtgcgcgt
      181 tgcaagattt cttcgcacag cttcatctgt gaaggcgtga ccattgacga ggaagtgttt
      241 gtggggcacg gggtgatgtt caccaacgat cgctatccgc gcgcagtcaa tgaagacggt
      301 tcgctgcaaa cggaaaatga ttgggtagtg gtacccacgc gagtcaggcg gggcgccgca
      361 attggcagca acgtcaccgt ggttgcgggc gtcgtaatcg gcgaaagggc tcttgtaggc
      421 gcgggtgcgg tagtgacgcg tgatgtgcct gattatgcaa ttgtggccgg tgtgccggcg
      481 cgggttattg gagacgtgcg gaggaagtag gcagtagaca gcagacaagt agatataaac
      541 cccgatcacc caaacactcg actacctgac taccaacgta caactactga caactgacaa
      601 ctgactaccg actaccgact tcgggcttct gactacggac tactgactac tgactgctcc
      661 attatgataa agatcggcgt aatcggttat ggctactggg gtccgaattt ggtgcgcaat
      721 ttcatggagg cgcccggctc gaccgtcgtc gctgtctgcg acctgcgacc cgaacgactt
      781 gagcagttaa caaaccgtca tccctcaatc aagagctgca ccaatagcga ggctctcctc
      841 aacgactcgc aaattgaagc gattgcaatt gccacccctg tctcgactca tttcgatctg
      901 gctctcgccg ccttgcgcgc tggcaaacat gtcctggtcg aaaaaccact ggccgcaaat
      961 tctgaacaag cgctgcagtt gattgcagaa gccgcggcat gcaagcgcgt gctgatggtt
     1021 gaccacacct ttgtctatac gggcgcggtc agaaagattc gcgaattgat cacggagaac
     1081 cacctaggtg agatctacta ctacgacgca gtgcgcgtta acctgggatt gtttcaacac
     1141 gacgtcaacg tgatctggga tctggcgatt catgatctgt cgatcatgga ttacgtcctg
     1201 ccctcacgtg cggttgcggt ttcagcaacg ggtatcagca acattcccgg gcaaccggaa
     1261 aatgtcgcct acatcacact cttttttgga aatccccaaa tcgcacacgt gcacgttaac
     1321 tggctcacgc ccgtaaaagt ccgtcatacg ctaatcggcg gcagcgacaa gatgatcctt
     1381 tacgacgatc tcgagcccag cgaaaaggtg aaagtctacg ataaaggggt ccgcgtttca
     1441 cagagccccg aagctgttta tgaaatgctg gtgagctatc gctcgggcga tatgtgggcg
     1501 ccgcgtttgg acgccaccga agctttgcac actgaagcgt tacacttcat tgattgcatc
     1561 caaaacaata agccgccggt aactgatggt cacgcgggac tgcgactggt gcgcatcgtg
     1621 gaagctgcgg agcgatcttt gcgcgcccgg ggtgagttag tggagattca ataagagtct
     1681 ctttcaaacc gggcggtcac agcgggacgc tcctactatt cagaacccca tgattccttt
     1741 tgtagatctc aaagcgcagt atctgacgat caaggacgag attgacactg ctattcgaag
     1801 tgtcgttgag agttgtcatt ttgtgctcgg cgaacaagtt gaggctttcg aaacagactt
     1861 cgccaggttt tgtgcgactg agtatgccct gggtgtgaat tccggcacga gtgcgctgca
     1921 tctcgctttg ctcgccgctg gagtaagagc gggtgatgaa gttattaccg ttagcaacac
     1981 gttcgtcgcc accgcagcag cgatttgtta tacgggcgcc agacctgtgc ttgttgaaat
     2041 ggaaccgcgt tcctgcacga tggatccggc aaagattgaa gccgcaatca cgccacgcac
     2101 caaagtgatc atgcccgttc atctctatgg cggttgcgca gatatggatc cgatacggga
     2161 aatcgccagc aggcgcaatc tgattgtgat cgaggatgcg gcgcaagccc atggcgccga
     2221 gtataaggac cggcgtgccg gcagcatgag cgacattgcg tgtttcagtt tctatcctgg
     2281 caaaaatttg ggtgcttacg gagagggcgg agcggtggtt acagataatt cccaatacgc
     2341 cgcaacaatc aaacgcttaa gggatcacgg ccagggtgag aagtactacc acgatgaagt
     2401 tggttacaac tatcggatgg aaggcattca gggtgcggtc ctgcgcgtga agctggcgca
     2461 ccttgatgat tggacagccg cgcgccggca gcatgccgcc acctatcgcc agtccctggc
     2521 tggctcagga atgcgtctgc ttgaaccgta tggtaattcg gcttggcata tcttccccgt
     2581 gttcacgcag cggcgtgacg agttgatcga gtatcttaaa tcccgtggtg tcgccaccgg
     2641 cattcattat ccgattcccg ttcacctgca gcgaggctac gctgatctcg ggtacaagcc
     2701 tggcgagttg ccactcaccg agcaggcttg tcgagaagta ctgtcgctgc cgatgtatgc
     2761 ggaactgcca aatgaggatt tgctggaagt agtcgccgcg attcacgagt ttgctgcaaa
     2821 tacttctcag cccaccgccg aagcgactcg tgcccactct tagtcttcgc aaccgatgcg
     2881 catctggatc gatcttgcaa attctccgca cgtccctttt ttccgcgcac tcataagtga
     2941 attcgtcgcc cgcgggcatc agtttgaggt aacggcgcga gagtttgccg agacagtgcc
     3001 gctggcacgg agcgcaggtc tggagccgac ggtgattggc gttcacggcg gacgcgagct
     3061 tacagccaaa gcgggaaacc tatttcagcg ggcctgggcg ttgcgagcct gggccagcgg
     3121 acgagctttc gatctggccg ttagtcacaa ctcctattcc cagatcctgg cagcgcgcgg
     3181 tctgggtttg cgctgcgtca cgttgatgga ctacgagcac cagcccgcga atcacctggc
     3241 gtttcgtctc gcatctacgg tgatcgtgcc gcgcgccttt cctgaggaag atctcagtcg
     3301 ttacggtgcc gtagctatta aagttaagcg gtacaatggc atcaaagagg acgtctacct
     3361 cgctgacttt gaaagtgacc ccgcgttcgc gggaaagttg cgcgaaatcg gcgcaaagct
     3421 cgagagcacg atcgtcgtag ttagaccgcc cgcgcgtgat gctctctatc atcggttcga
     3481 gaatgatttg tttgaggggt tgctaaagcg gttgagtgag acgcctcagg tgacaacaat
     3541 cctgcttccg cgtaatgccg gtcaacgaac cacctatgca gcacgggttg gcccgagaac
     3601 gatcatgcct gaggaacctt tggat
//
LOCUS       DASUEX010000558         2415 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_529268, whole genome shotgun sequence.
ACCESSION   DASUEX010000558 DASUEX010000000
VERSION     DASUEX010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2415
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_529268"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(299..1084)
                     /locus_tag="VFV61_09335"
     CDS             complement(299..1084)
                     /locus_tag="VFV61_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015351969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX4900723.1"
                     /translation="MTTNIFAEKILSGRVAFVTGGGTGITGGVARALAEAGARVALVS
                     RKLEHLQPAAEAINANGGEAIALAADVREPEAVAAAIAATIERFGKLDIVLNGAAGNF
                     LCAAEELSPNGFGTVVDIDLKGTFNVCRAAFAELKKNRGQILNISATLHYLGTPMQLH
                     VSAAKAGVDALTRNLAVEWGRHGIRVNAIAPGPIGDTEGMKRLVPEPIKEKLRQRIPL
                     GRFGEIADIEKAAVFLCSEAAGYINGTVLVVDGGHWLAANRLI"
     gene            complement(1092..>2415)
                     /locus_tag="VFV61_09340"
     CDS             complement(1092..>2415)
                     /locus_tag="VFV61_09340"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX4900724.1"
                     /translation="LFAEPLPENGIGLDEILTRFRNDVAPHAMTVPSPRYFGQFNPTP
                     LPIGVWADALAAALNQNAGAWRNGPTSAMIEARVMRWLCELVGYGDESFGTLASGGSE
                     ANLIALKCARDAVSAAIVHRGMRSAPADLVIYASEQCHYSLEKSADILGMGREQLRKI
                     PTDERFHIRTDLLRVEIENDRRAGLLPCCVVGVAGTTSTGVIDPLPELATIARENSCW
                     FHVDAAYGGVLAFSAPHKAKLHGIEHADSITFDPHKWMFVPFACGAVLVREGGRILRD
                     AFDISPEYLSEDRGGADVEYDFFRYGQMGTRRFNSLKLWMAWKFLGRNGYAELIEKQI
                     ALTEYFARKIDGLKDFERLGEVETAVCCFKFLPPPARQKDGGYQDRLQQRLQQLIERS
                     GEAWITTTVLHGRRALRVNINSFLTESRHVDDLIAVLERQSATLLNET"
BASE COUNT          522 a          713 c          625 g          555 t
ORIGIN      
        1 atggatccgc tgggaccgtg ggtgcatcag ccggccgggc gtttgctttt ttgagcgtgt
       61 cggtggcggt ggttggcttt tcaggcgggg tggaggcaga aactggctct tgtgccttgg
      121 ccgagacggt aaggaaacag attgttagga ataggaaggc tcgcatcaaa tccttggtcc
      181 gttgtcagtt gttcgttgtt agttgttcgt tgtcagtggt cggttgttcg ttgtcggtct
      241 gccaccactc atcagtaact actgactacc atttactgac tactaaccgc taactagttc
      301 atatcaatcg gttcgcggcc aaccaatgtc cgccatcaac cacgagtact gtgccgttga
      361 tataaccagc ggcttccgaa caaagaaaga ccgcagcctt ttcgatgtca gcgatttcgc
      421 caaaccgacc tagcgggatt cgttgacgca acttctcctt gatcggttcg ggcaccaggc
      481 gcttcattcc ttcagtgtcc ccaatcgggc ccggagcgat agcgtttacc cgaatgccat
      541 gccggcccca ttccaccgcc agattcctgg tgagcgcgtc gactcccgct tttgctgcag
      601 agacgtgcag ttgcattggc gttcccaggt aatgaagcgt ggcgctgata ttcaggatct
      661 gtccccggtt cttcttgagc tcagcgaacg ctgcgcgaca tacgttgaac gtgcctttga
      721 ggtcaatgtc aacaacggtg ccgaacccgt ttggtgagag ttcttcggcg gcacacaaga
      781 agtttcctgc cgcaccattt aatacaatat cgagcttgcc gaaccgctcg atcgtcgctg
      841 caatcgctgc ggcaacggct tcaggctcgc gcacgtcggc ggccagggca atagcttcgc
      901 ccccattggc attaatcgcc tcggcggcgg gttgtaggtg ttctaacttg cgactcacga
      961 gcgccacgcg cgcaccagct tcagcaagcg ctcgagcgac gcccccagtg atgcccgttc
     1021 cgccgccagt gacaaaagcg acacgcccac tcaatatttt ctcggcaaag atgtttgtgg
     1081 tcataatttt gttaagtctc gttcagtaaa gtcgcacttt gtctttccag caccgcgatc
     1141 agatcatcca cgtggcgaga ttcggtaagg aagctgttga tgttgacgcg caacgcccgg
     1201 cgcccatgca aaacagtcgt tgtgatccat gcttcgccgc ttctttcgat taactgttgc
     1261 aggcgctgct gcaagcgatc ctgataacct ccgtctttct gccgggcagg cggaggcaga
     1321 aatttgaaac aacataccgc agtttcaact tcgccaagtc gctcaaaatc tttcaggcca
     1381 tcaatcttac gagcgaaata ctcagtcaaa gcaatctgct tctctatcaa ttccgcgtaa
     1441 ccgtttctcc cgagaaactt ccaagccatc cagagtttca gtgagttaaa gcgccgcgtg
     1501 cccatctgtc cgtaacgaaa gaaatcgtac tcgacgtccg caccgcctcg atcttcgctc
     1561 aggtactccg ggctgatatc gaacgcgtcg cgcagtatcc gtccgccttc acgcactaac
     1621 actgcgccgc aagcgaacgg cacgaacatc cacttgtgcg gatcaaatgt gatcgaatcc
     1681 gcgtgttcaa tgccgtgaag tttggctttg tgtggcgccg agaacgccag cacaccaccg
     1741 taagcagcat ccacgtgaaa ccagcaactg ttctcgcgcg cgatggtcgc cagctccggc
     1801 aacggatcga ttactccggt cgaggtagtg cctgccacac caacaacgca gcagggcagc
     1861 agtccagcgc gtcgatcatt ttcgatctct acgcgcagca aatcagtgcg gatgtgaaaa
     1921 cgctcatcgg taggaatctt ccgcagctgt tcccggccca ttcctaaaat gtctgcactc
     1981 ttctcaagtg aatagtgaca ttgctcagaa gcgtagataa ccagatccgc gggcgcgctc
     2041 cgcatacctc gatggactat cgcggcgctg accgcgtcgc gggcacactt caaagcaatc
     2101 aggtttgcct cggaaccgcc gctcgccaaa gtcccaaaac tctcgtctcc ataaccaacc
     2161 aattcacaca gccaccgcat cacgcgcgct tcaatcatgg cggaggtagg tccattccgc
     2221 caggcgcccg cattttggtt tagagccgct gccagggcgt ccgcccagac tccaatgggt
     2281 aatggtgtgg gattgaactg cccaaagtaa cgcgggctcg gcactgtcat ggcatgaggt
     2341 gcaacgtcat tcctaaaacg cgtcaggatt tcgtcaagtc caatgccgtt ttcgggaagc
     2401 ggctcggcga acaat
//
LOCUS       DASUEX010000559         3023 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_780561, whole genome shotgun sequence.
ACCESSION   DASUEX010000559 DASUEX010000000
VERSION     DASUEX010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3023
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_780561"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..665)
                     /locus_tag="VFV61_09345"
     CDS             complement(<1..665)
                     /locus_tag="VFV61_09345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEX4900725.1"
                     /translation="MFIDSHAHIDGPEYDADRPEVILRAREAGVTTILNVGTGDPHSG
                     AFERAVQLANDHQEIFAAIGVHPHDAKLYDDAAEARILSLIAAHPRILAWGEIGLDYH
                     YDNSPRDIQRRVFQRQLEAARVCDLPVVIHTRSADADTIEILRHAYQRTTRGGIMHCF
                     GGSLELAQAAIDLGFYISFSGNITFKKAGDLRAVAAQIPRERLLIETDCPYLTPVPFR
                     GKR"
     gene            complement(675..1442)
                     /locus_tag="VFV61_09350"
     CDS             complement(675..1442)
                     /locus_tag="VFV61_09350"
                     /inference="COORDINATES: protein motif:HMM:NF039614.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HEX4900726.1"
                     /translation="MKRAIVCQPASTMRHGLTRSDLGLPDYAHALVQHEEYCAALIRC
                     GLEVFSLNPVEAFPDSVFVEDVAVLSGQSAILTRPGATSRRGEVSLIEPHLRSLIRSV
                     KRIEEPGTLDGGDVCEAGNHFFIGISERTNEAGARQLGQFLEALGFSYDCIDIRDIRE
                     LLHLKSALTYLGDNRLVVTDALAGLKEFAGYEIVHVAAAEDYAANCVRVNDHVLIAKG
                     YPAFSATLAALGYQTIPLDMSEFQKLDGGLSCLSLRF"
     gene            1605..2189
                     /locus_tag="VFV61_09355"
     CDS             1605..2189
                     /locus_tag="VFV61_09355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900727.1"
                     /translation="MIKRIGAAILGLTFAFGAFGATAVTAVAKNDKDKDKRNRRWDRN
                     QDWRRNDDDRRWRGRNRGTDRPHGRGYNNGRYGYPGNNNGRYGNGRYGNGRYDNGRYD
                     NGRYGNYGGNSYELNRGYQQGLSTGSSDARRGQSYNPQRSRYYRNPSSYEFREGFVRG
                     YDQGYRQYAGYNNNGYYRNNNGSVWSQILGIPFQ"
     gene            2383..2907
                     /locus_tag="VFV61_09360"
     CDS             2383..2907
                     /locus_tag="VFV61_09360"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX4900728.1"
                     /translation="MKKWLIRIVIVFVVLIAALFIAGALLPEEHHASKTLITKQSPQA
                     LWDAISDHATEAQWRADVANVTSLGEREGKPVWRENYKDGNSVTLITTESKPPTRMVR
                     ELTDLDGPFSGRWEIDIAPTPAGSNITVTEIGKVSNPLFRFVSKFIIGHTTFMERYLK
                     GLAGKFGEQPQFAN"
BASE COUNT          724 a          818 c          816 g          665 t
ORIGIN      
        1 tttcgctttc cgcgaaaagg aactggggtt agataaggac aatcggtttc tattagaaga
       61 cgttcgcggg ggatttgagc cgcgacggca cgcagatccc cggctttctt aaacgtaata
      121 tttccggaga aggaaatgta gaagcccaga tcgattgcgg cctgcgccaa ctccaacgag
      181 cctccaaaac aatgcatgat gccgccgcga gtcgttcgct gataggcgtg acgcaggatc
      241 tcgatggtgt ctgcgtcggc gctgcgcgtg tgtatcacta ctgggagatc acaaacccgg
      301 gccgcctcca gttgcctttg aaatacccgg cgctgaatat cacgtggcga attgtcgtag
      361 tgatagtcga gaccaatctc accccaagcc agtatcctgg gatgggctgc aattaaactg
      421 aggattctcg cctccgcggc atcgtcgtag agtttggcgt cgtgagggtg gacaccgatg
      481 gcggcgaaaa tttcctggtg gtcgttcgcg agttggaccg cccgctcgaa tgctccgctg
      541 tgcggatcgc ccgtgccgac attgagaatt gtcgttaccc ctgcttcacg cgcgcgcaag
      601 atcacttccg ggcgatcggc gtcatactcg ggcccgtcaa tgtgggcgtg ggaatctata
      661 aacatcaatc gatttcagaa tcttaaagag agacagctta ggccgccatc aagcttttga
      721 aactcgctca tatcgagcgg aattgtctga tagccgagag cggccagcgt ggctgaaaag
      781 gccggatagc ctttggcaat taacacatgg tcgtttacac gaacgcagtt ggcagcgtag
      841 tcctcagcag cggcaacgtg cacgatctca tatccggcaa attccttaag gccggcaagg
      901 gcgtctgtta ccaccagacg attatcgccg agatacgtca gcgcgctttt caagtgaagg
      961 agctcgcgga tgtcgcgaat gtctatgcag tcataactaa aaccgagtgc ttctagaaat
     1021 tgaccaagtt gtcgcgcgcc agcttcattg gttcgttccg agatcccaat aaagaagtgg
     1081 tttcctgcct cgcagacgtc gccgccatcg agggtgcccg gttcctcaat tctcttaacc
     1141 gagcgaatca gcgaccttag gtgcggctcg atcaggctca cctcgccccg acggctggtt
     1201 gcgcccggtc gcgtcaggat tgcagattgc ccgctaagaa cagcaacgtc ttcgacaaag
     1261 acagaatcgg gaaaggcttc gacggggttt aagctaaaca cttccagtcc gcaccgtatt
     1321 aatgcggcgc agtactcctc gtgttgcacc agagcgtgtg cgtagtccgg caaccccagg
     1381 tcagatctcg tcaatccgtg acgcatggtg gaggccggct ggcaaactat tgctcttttc
     1441 attggttata gcctactccg ttcagagcgt atcggactgt attccatatc cgtacttaaa
     1501 tcgcactttt tacaccatat gagcactgtc ccggctagga aaaccacgca acgattgctg
     1561 gcataaatgt tgccgaatct atcggcgaga gggagggtac ctaaatgatc aagagaattg
     1621 gggcagctat tctaggtctc acattcgcct tcggcgcttt tggggcgact gcggtgacgg
     1681 ctgttgcaaa gaacgataaa gacaaagata agcggaaccg ccgttgggat cgcaatcagg
     1741 actggcgtcg taacgacgac gaccggcgct ggcgcggccg aaaccgtgga accgaccgtc
     1801 cacacggacg tggctacaat aacggccgct acggttaccc cggcaacaac aatggccgct
     1861 atggtaatgg ccgctatggt aatggccgct acgacaatgg ccgatacgac aatgggcgct
     1921 acggaaacta cggtggcaat agctatgaac tgaatcgcgg ttaccagcag gggctgagca
     1981 ccggatcaag cgatgcgcgc cgtggtcaga gctacaaccc gcaacggtct cgctactatc
     2041 gcaatccatc atcgtacgag tttcgtgaag gattcgtgcg cggttacgac cagggttatc
     2101 gtcagtatgc cggctacaat aacaatggct actatcgcaa caacaatgga agtgtttgga
     2161 gtcagattct tgggattccc ttccaataaa ccatacgctt gaaggcggat ggtcaagcac
     2221 caccccgacc cgtattcgag cacttgagga atgccgcgcc tgtcgcggca tttttcattt
     2281 cttgaatctg cttttggctc cgatactgtt tccacgtcat cgcttcaccc tattactcgc
     2341 atcacgattc aggctattat ccggcccaca aaggagaggc ctatgaaaaa gtggttaatc
     2401 cggatcgtga tagtgttcgt ggtgctgatc gcggctctct ttatcgcggg cgccttactt
     2461 cccgaggaac atcacgcctc gaaaactctc attactaagc aatcgcctca agcactttgg
     2521 gacgcaatca gtgatcacgc aaccgaggcg caatggcggg cagacgttgc gaatgtgacc
     2581 tcactgggtg aacgcgaagg caaacctgtc tggcgggaaa actacaaaga cggcaacagc
     2641 gtcactctca taacgactga gtcgaagcca cctacgcgaa tggtgcgaga gctgacagat
     2701 cttgacggtc ctttcagcgg ccgctgggaa atcgatatcg cccctacgcc agccgggagc
     2761 aatatcaccg taactgaaat cgggaaagtc tctaatcccc tcttccgctt cgtttcgaag
     2821 ttcatcattg gccacacaac tttcatggag agatacctga agggtctggc cggaaaattc
     2881 ggcgaacagc cacagtttgc caactgactt cctaatttcc atgaaagtta atttcccagg
     2941 tgagaggaag ccatgaggga ttattcctca agaactagca gccagcaggc acgccaactg
     3001 acgggttgcg ttaccggcca cgg
//
LOCUS       DASUEX010000560         2663 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_97500, whole genome shotgun sequence.
ACCESSION   DASUEX010000560 DASUEX010000000
VERSION     DASUEX010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97500"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1133
                     /locus_tag="VFV61_09365"
     CDS             <1..1133
                     /locus_tag="VFV61_09365"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX4900729.1"
                     /translation="MKGLALQRAKRLLGEGLLTSEGDFHRRQKRLAQPAFHRARIASY
                     ASVMTNYAAEMSGRWRDGEILDVSQEMMRLTLAIVGKTLFDADVERDATDVGEAMSVV
                     MDLFNTISIPFYDLLIKLRLPQTRRFDLAKERLDSIIYRLIEERRLSGEDRGDLLSML
                     LLAQDPEEQGSRMTDEQLRDEAMTIFLAGHETTANALTWTWYLLSQHPEVEAICHQEI
                     DRVVGSGLPDLDSISNLPYTEKVLLESMRMFPPAWALGRMALNDCEIGGYLVPKGSLV
                     LMSQYVMHHDSRYFPEPDRFDPERWAPELREARPQFSYFPFGGGPRRCIGEGFAWSEG
                     ILLLATLARHWQMRLVANHPVELRPVITLRPKYGMKMVVKRR"
     gene            1295..1480
                     /locus_tag="VFV61_09370"
     CDS             1295..1480
                     /locus_tag="VFV61_09370"
                     /inference="COORDINATES: protein motif:HMM:NF015899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HEX4900730.1"
                     /translation="MAISQELLEILACPACKAKVELKPDGSALKCVECKRVYPVRDDI
                     PVMLIDEATIEDDQSQS"
     gene            1487..2632
                     /locus_tag="VFV61_09375"
     CDS             1487..2632
                     /locus_tag="VFV61_09375"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEX4900731.1"
                     /translation="MNNLREIVESSPQFRRLAMQGLRNQEQPEPLGPALWQWAEVQKV
                     LVVRLRSIGDTVLSTPSIYALKRFLPQARIDVLVEDWVAPVLDGFAYVDNVITMARKS
                     VASRARVARQLRATRYDVVYNLHGGSTATFLTRATGAPHRVGYADYQYARLHQHLAPT
                     SALLWGKPKTHSVEQQLALLGWTGVPVTDRPPTRLTVTDKAAASIAARLSSNGIDESD
                     EFVLLHPAAAFATKQWAADRFARVAEALADYGLRIVAITTEKEAELAQSLQAKSAAPI
                     LSFTDLSLPEVTALAARARLFVGNDSGVAHIAVAVGAPAVVIFGSSNVDHWRPWSSAP
                     AEIVREDLECQPCHGYFCEEFAEPECIKRVPVEKVLAAASRILERRQ"
BASE COUNT          599 a          684 c          782 g          598 t
ORIGIN      
        1 tcatgaaggg ccttgcgctg caacgcgcaa aacgactcct gggcgaaggc ttgcttacca
       61 gtgaaggaga ctttcatcgc cggcaaaaac gtttggccca gccagccttc catcgggccc
      121 gcatcgcatc ctatgccagc gtgatgacca actatgccgc ggagatgagt ggcagatggc
      181 gtgatggtga aatcctcgat gtatcgcagg agatgatgcg acttacgctc gcgatcgtgg
      241 gaaagactct ttttgatgca gacgttgaga gagatgcaac cgatgttggt gaagcgatga
      301 gtgtggtgat ggatctcttc aatacaatct cgattccgtt ttacgatctg ctgatcaaac
      361 tgagactgcc gcaaacgcgc cgctttgacc tggctaaaga gcgattggat tcgatcatct
      421 accgcctgat agaagagcgc cgtttgagtg gcgaagatcg aggcgacctc ttgtcaatgt
      481 tgttactggc ccaggatcca gaggagcagg gctcgcggat gactgatgag caactgcgcg
      541 atgaggcgat gaccattttt cttgcgggac acgaaaccac ggctaatgct ctgacctgga
      601 cctggtacct tttgtcacaa catcctgagg ttgaagcaat ttgtcaccag gagatagatc
      661 gggtagttgg ctcgggtctg cccgatctcg acagcatttc caacttgcct tacacggaga
      721 aggttttgct ggaatcgatg cgaatgttcc cgcctgcatg ggcactgggt cgcatggcac
      781 tgaatgattg cgagattggt gggtacctgg ttccgaaggg atcgctcgta ttgatgagcc
      841 aatacgtgat gcatcacgac tcgcgctatt ttcctgaacc tgatcggttt gacccggagc
      901 gttgggcgcc agagctgcga gaggcacgac cacaatttag ttacttccca tttggcggtg
      961 gacctcggcg ctgcatcggc gaaggctttg cgtggagcga aggaattctg ttgcttgcaa
     1021 cgctggcgcg gcattggcaa atgcgattgg tcgcgaatca tcccgtcgag ttgaggcccg
     1081 taatcacact tcgaccaaag tatgggatga agatggttgt gaagaggagg tgacgagtga
     1141 caagtgactc aaaacccgca acccaaaact cgaaactgaa accgaagccc aactgtcacc
     1201 tgtccccgtt ctgctgtgat aagcttcgaa cttccccagg cctttcaggt gttccagttc
     1261 gaaccaggaa tcaagcaatg cgaaaggtaa tatcatggca ataagtcagg agttactgga
     1321 gatactcgcc tgtcctgcgt gcaaagcgaa ggttgagtta aagcctgacg ggagtgctct
     1381 caaatgcgtt gaatgcaaac gagtctatcc cgtgcgcgat gacattccgg tgatgttgat
     1441 tgatgaagcc actatcgaag acgatcaatc acagtcttaa ttcccgatga ataatctgcg
     1501 cgaaatcgtt gagtcctcac cgcagtttcg caggctggca atgcagggtc tcagaaatca
     1561 ggagcaaccc gaaccgctcg gcccggcact ttggcagtgg gcggaggtac agaaggtgct
     1621 ggttgtgcgg ttgcgttcca tcggcgacac agtgctctca acaccctcaa tctacgcgct
     1681 gaagcggttc cttccgcaag ctcgtatcga cgtgttggta gaggattggg tggcgcccgt
     1741 gctggatggc ttcgcgtacg tggacaacgt gatcacgatg gcccgaaaaa gcgtcgcctc
     1801 tcgtgcgcga gtcgcgcgcc agcttcgcgc tacccgctac gatgttgttt acaatcttca
     1861 cgggggcagc acggccacat tcttaacaag agccacaggc gccccgcacc gtgttggcta
     1921 tgcagactat caatatgcac gtttgcatca acatctggcg cccacttcag ctttgctctg
     1981 gggcaaacca aagacccatt ccgtggaaca gcaactcgcg ttgctcgggt ggactggagt
     2041 gccggttacc gatcgcccgc caacgcgcct gacggtaact gataaagctg cggccagcat
     2101 tgcagccagg cttagttcga acgggatcga tgagagcgat gaattcgttc tgctgcatcc
     2161 ggcggcggca tttgccacca agcagtgggc tgcggatcga tttgcgcgcg tcgctgaggc
     2221 gttggcggat tatggtctgc gaattgtcgc gatcactact gaaaaagagg ccgaactcgc
     2281 gcaaagcctg caagcgaagt cggccgctcc tatcttgagt ttcactgatc tttcgctacc
     2341 cgaagttacc gccctggccg cgcgggcgcg cctctttgta ggcaatgaca gtggagtcgc
     2401 tcacatagcg gtggccgttg gcgcgcctgc agtggtgatt ttcggatcct caaatgtcga
     2461 tcactggcgg ccgtggtcca gtgctccagc cgaaatagtg cgcgaggatc tggagtgcca
     2521 accctgtcat ggatattttt gcgaggaatt tgctgagccg gaatgtatta agcgggtgcc
     2581 ggtggaaaag gtattagcgg cagcgtcgcg gattttggag cgccggcagt agactaagca
     2641 ggccaacagt gactgaggaa aaa
//
LOCUS       DASUEX010000561         3934 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_780714, whole genome shotgun sequence.
ACCESSION   DASUEX010000561 DASUEX010000000
VERSION     DASUEX010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3934
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_780714"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(90..1751)
                     /locus_tag="VFV61_09380"
     CDS             complement(90..1751)
                     /locus_tag="VFV61_09380"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX4900732.1"
                     /translation="MEHQASHDQRILILAPTGRDGDLTSRVFGHYRLQSEVCAGPWDL
                     CERLQGGAGLVFVTGEALTSSLVRSLVEVLKQQPTWSDIPLVILTSGGGDLPANAEEL
                     ATLVETGNVTLIERPVRVMTLVSAVESALRARRRQFDVRDYLAAEQRAKDELTRAIQR
                     AEEANSLKDEFLATVSHELRTPLMAVLGWAHLLRSNNLDETGQRRAIETIERNARAQQ
                     QLIEDLLDVSRIITGKLRLDVRPVVPEAFIAAAVESVRPAADAKEIKLEIISDPNIGT
                     LSGDAGRLQQVVWNLLSNAIKFTPRAGRVRLEVLRTSSHVEISVHDSGQGISADFLPY
                     VFERFRQADMKTTRAHGGLGLGLAIVRQLVELHGGTVKVMSDGEGEGAVFVVKLPVLP
                     VYANSPARGQANLSDIEADAVVDCASNLDGLKILVVDDEADTCELLRSLLTKCGADVT
                     AARSASEAFRLFQLVIPEVLVSDIGMPHEDGYELMRKVRALPEESGGKVPAVALTAYA
                     RAEDRVRALRAGFQMHVSKPIELTELVAIVASLGDRLKKASDGAD"
     gene            complement(1754..3277)
                     /locus_tag="VFV61_09385"
     CDS             complement(1754..3277)
                     /locus_tag="VFV61_09385"
                     /inference="COORDINATES: protein motif:HMM:NF018455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HEX4900733.1"
                     /translation="MKKNNPSSSSGETNFDPPRTTMGVGGLDDILNGGLPANRIYLIE
                     GEPGTGKTTVALQFLMEGARLGESGLYITLSETKEELESVAHSHGWSLDGFSVYEMAP
                     AEENLKPESQYTIFHPSEIELGETTSAVLQEVERVKPHRVVFDSLSEMRLLAREPLRF
                     RRQILALKQYFAGRHSTVLLLDDKVYGGQDLQVHSIAHGVISLDHLAVEYGAERRRLR
                     VTKLRGSRFRGGYHDFTILTGGVAVFPRLVAAEHHEEFKRESITSGINELDLLLGGGL
                     DRGTATLILGPAGSGKSTLGAQFAAATATRGELTAAFVFDELKETFIGRAAATGTDVR
                     NFMNADLITVQQVDPAELSPGQFAHRVRNAVDVNGARVIIIDSLNGYLNAMPDERFLT
                     IQMHELLTYLNQKGVATILVMAQHGFLGTSMATPVDISYLADTVMMLRYFETDGAVHR
                     AISVIKKRSGPHEHTIREFNITANGIRIGPPLSQFRGVLTGVPIYEGSRDVLSEKGR"
     gene            complement(3482..>3934)
                     /locus_tag="VFV61_09390"
     CDS             complement(3482..>3934)
                     /locus_tag="VFV61_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900734.1"
                     /translation="TWVAEMFMSGTAHERRKLELERARGLTPEVKRYNEIKAGVITGS
                     AGLALMLFLAVMMEGIIIGGKIPDDTAEILSRVWIAGVIPLFIGIALIINGVFVSKKI
                     VEAQNRHTLPERDLKPDLLRSPNTAEFVPASFSVTEETTRQLRDSERT"
BASE COUNT          858 a         1158 c         1030 g          888 t
ORIGIN      
        1 atcgaacgct acttcagaaa gtcgcctgtg aatgttacgg tgcgctggcc ggtcacgtca
       61 cagaatgagt tcccaataac agtgtattct caatcagccc cgtcggatgc tttcttcaag
      121 cgatcgccaa ggctggccac aatcgccact aattcagtta actcaatcgg cttagaaacg
      181 tgcatttgga aaccggcacg cagtgcacgc acgcggtctt ccgctcgtgc gtacgctgtt
      241 aacgccaccg ccggaacctt gccaccgctc tcctccggaa gcgcgcgcac tttccgcatc
      301 aactcgtaac cgtcttcgtg gggcattcca atatctgaaa ccagcacctc cggaatcacg
      361 agttggaaaa gtctgaaggc ctcactcgcg gatctagcgg ccgtcacgtc agcgccacac
      421 tttgtcagta atgagcgcag cagttcacac gtgtctgctt catcgtcaac caccagaatt
      481 ttcagaccat cgaggttact tgcacaatct acgaccgcgt cggcttcgat gtccgacaaa
      541 ttcgcctgcc ctcgcgctgg cgaattcgca taaacgggca agacaggcag cttgacgaca
      601 aaaacagcgc cctccccttc tccgtcgctc attactttga cggtgcctcc gtgaagctcc
      661 actaactgcc ggacgattgc cagccctagt ccgagaccgc catgtgcgcg agtggtcttc
      721 atgtccgcct ggcgaaagcg ctcaaagacg tacggtagga aatcggcact gataccctgg
      781 ccgctatcat gaaccgagat ctcaacgtgg gagctcgtgc gcaatacctc cagtcttact
      841 cgtccggcgc gcggtgtgaa cttgatggcg ttcgaaagta agttccagac aacttgctgc
      901 aaacgtcccg cgtcgccgga gagcgtaccg atgttcgggt cgctgataat ttcgagctta
      961 atctctttcg cgtcggcagc gggacgcacg gactcgaccg cggctgcgat aaaggcctcc
     1021 ggcaccacgg gccgcacgtc cagacgcaat tttccggtga tgattcgcga tacgtcgagc
     1081 aggtcctcga tcagttgttg ctgcgcccgc gcattacgtt caattgtctc tatggcccgc
     1141 cgttgcccgg tttcgtccag gttgttagag cgtagcagat gcgcccaacc aagcacggcc
     1201 atcagcggtg tccgcagctc atgggaaacc gttgccagaa attcatcctt caggctattc
     1261 gcctcttccg cgcgctggat ggctcgcgtc aactcgtcct ttgcacgctg ctcagcggcg
     1321 aggtaatcac ggacgtcgaa ttgacgccgg cgggcgcgca gcgcggactc cacggcgcta
     1381 actaatgtca tcacgcgcac cgggcgttcg attaacgtca cgtttccggt ctcaacgaga
     1441 gtcgcaagct cctcagcatt ggccggtaag tcaccgccgc cactggtgag gatcacaagc
     1501 ggaatgtctg accaggtggg ctgctgtttg agcacttcga cgaggctccg tactagagac
     1561 gacgttaaag cttcgccggt aacaaaaacc agacccgcgc caccttgcaa ccgttcgcac
     1621 aaatcccagg gtccggcaca aacctctgat tgcaagcggt agtgaccaaa gacccgcgag
     1681 gttaagtcgc cgtctcttcc ggtcggcgcg aggatcaata ttctttgatc gtgactagct
     1741 tgatgctcca ccgtcatcgt cctttttcac ttaatacgtc cctcgaccct tcgtaaatgg
     1801 gtactccagt cagcacgccg cgaaactgac tcaagggtgg gccgatgcga ataccgttcg
     1861 cagtaatatt aaactcacga attgtgtgtt cgtgcgggcc gctgcgtttc ttgatcaccg
     1921 agatcgcccg atgcactgcg ccatcagttt cgaagtaacg caacatcatt acggtgtcgg
     1981 ccaaatagct gatatccacc ggagtcgcca tcgaggtgcc gaggaacccg tgttgcgcca
     2041 tcacaagaat cgtcgccacg cccttttggt ttagataggt cagcaactcg tgcatttgga
     2101 tcgtcaggaa acgctcgtcg ggcatagcgt tcaaataacc attcaaactg tcgataataa
     2161 tgacccgcgc accgttgacg tcgactgcgt tgcgtactct atgagcgaac tggccgggag
     2221 aaagttcggc aggatccacc tgctgaaccg taatcagatc agcgttcatg aagttgcgaa
     2281 catccgtacc agtagccgcg gcccgcccga tgaaagtctc tttcagttca tcgaaaacga
     2341 aagcggctgt gagttcgccc cgcgtagcag ttgcggctgc aaattgggct ccaagcgtcg
     2401 atttgccgga tcctgccggg cctaaaatca gcgtggccgt tccgcgatct aaccctccgc
     2461 ccagtaatag atccaattca ttaatgccgc tcgtaatcga ctcgcgtttg aactcctcgt
     2521 ggtgttcggc agccaccaga cgcggaaaca cagccacgcc acctgtaaga attgtgaagt
     2581 cgtgatagcc cccacggaag cgggagccac gcaattttgt tactcgcaaa cgtcgccgct
     2641 cggcgccata ttcaactgcc aggtggtcca gcgaaatcac gccgtgggca atcgaatgca
     2701 cttgcaaatc ttgtccgcca tagaccttgt catccaggag caggaccgtg ctatgccgac
     2761 ccgcaaagta ttgcttgagc gccaaaattt gacgacggaa acgcagcggc tcgcgtgcga
     2821 ggagtcgcat ttccgaaagt gagtcgaaca cgacacggtg aggcttgact cgttccacct
     2881 cttgcagcac cgcgcttgtc gtctccccta gctcaatctc ggacgggtga aagattgtgt
     2941 attgggattc cggtttaagg ttttcttccg ctggggccat ttcatacacg ctaaaaccgt
     3001 cgagcgacca accgtgagaa tgagcgactg actccaactc ttctttggtt tccgaaagcg
     3061 tgatgtagag ccccgactct cccagccgcg caccttccat caggaattgc aaagcgacgg
     3121 tagtcttacc ggtcccgggc tcaccttcga tgaggtagat ccggtttgcc gggaggccac
     3181 cattaagaat gtcgtcaaga ccgccgacgc ccatcgtcgt ccggggcggg tcgaaattgg
     3241 tctctccaga gctggaggac ggattgtttt ttttcatgac tgatccaacg ggcgtcttgg
     3301 aattcgacac tcctccggaa gttctgggac ggcgacagtg gcttatcagg gggtgcgagg
     3361 catcttatac cctgaacggg aatttttgtc gaagcgatac cgtcatcgtc gattgacgat
     3421 tgccgagtac caattagcct gaagcgaaag gagtcaattg gcaatcttaa ttgtcaattg
     3481 tctaagtccg ttcggagtcg cgcaactgtc tggttgtttc ttccgttacg ctgaaacttg
     3541 cgggtacgaa ctctgcagtg ttaggcgagc gcaggagatc aggtttgaga tccctttcag
     3601 gcagcgtgtg gcggttctga gcttcgacga ttttcttgct tacaaagact ccgttaatga
     3661 ttagggctat tccgatgaac agcggtatca cgcccgcaat ccagacgcgg ctcagaatct
     3721 cagctgtgtc atcggggatt ttgccgccaa taattattcc ttccatcatg actgcgagga
     3781 aaagcatcag cgcgagaccg gcacttcctg tgatcacccc agccttgatt tcgttatagc
     3841 gtttgacttc tggcgtcaga ccacgagccc gctccagttc cagcttgcgc cgctcgtgcg
     3901 cggttcccga cataaacatc tcagccaccc aggt
//
LOCUS       DASUEX010000562         2419 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1686494, whole genome shotgun sequence.
ACCESSION   DASUEX010000562 DASUEX010000000
VERSION     DASUEX010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1686494"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            177..914
                     /locus_tag="VFV61_09395"
     CDS             177..914
                     /locus_tag="VFV61_09395"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HEX4900735.1"
                     /translation="MAYGKLGWMPVTDSQTEHVSEVEAALLDAELFMKYQAPERAIKR
                     LRTALEISGRSIKLRERLREICASYKQNEEAARQCLALASLYIEREDFDTAHDRLLEA
                     KQFDGRINIARGLEAIRRARRPDLMPVPAPEANFERRHFTLAGDLSTISVFDAIQVIE
                     NARLNGVLTLSGEKHQGDVKFNEGRIVDAACGTNVGDPAFQQVVEITSGTFEFQKTAE
                     QFPVRIQAVSNTNLILDTLRMLDERNQ"
     gene            complement(917..1714)
                     /locus_tag="VFV61_09400"
     CDS             complement(917..1714)
                     /locus_tag="VFV61_09400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900736.1"
                     /translation="MTDRTYLTYTSSMSLEFALQYTCPLRANYSERHLREWGRLSSLH
                     AKLTTGDASAPSIDKLQWVENASAEIERMQEATIICTHCPACLPFDTAGDGESVGCLG
                     RINYPVEAQFEKFLADRVQLALDTLDKEDQPRLLRILVDPESPFDGEATKELRRVVTE
                     DGLRFFELRVPIVLTREAARLTSDNIFDLLAGFRSEDSETTTYTRELPFEAAADYYDF
                     LDLVLRSELSQSEVARLTSRSRNYSQFLRLLTAIEKAEALNTRVLID"
     gene            1780..1950
                     /locus_tag="VFV61_09405"
     CDS             1780..1950
                     /locus_tag="VFV61_09405"
                     /inference="COORDINATES: protein motif:HMM:NF015899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HEX4900737.1"
                     /translation="MAVSQELLEILACPACKAKVELKPDGNALKCVECKRVYPIRDDI
                     PVMLIDEATIEE"
     gene            1955..>2419
                     /locus_tag="VFV61_09410"
     CDS             1955..>2419
                     /locus_tag="VFV61_09410"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEX4900738.1"
                     /translation="MNSLRDIVELSPQLRVIETQANYGSEAQALAPPRWDWSAVRKVL
                     LVRLRSIGDTVLATPSLYSLKRFLPNVKVDILVEDWVAPLLNNHPHVDNVIVLERGGF
                     MTRARVARELRAANYDVVYNLHGGTTATFLTRATGARYRVGFKTYQYAQLHNH"
BASE COUNT          526 a          699 c          683 g          511 t
ORIGIN      
        1 cgctcgattc ttcgtttcat ccaccggttc attgtagttc gccaatcctt tctaatcggg
       61 gcttacgatt catggacctc gcaggttgca tgccaatcga atctccccac tcgtaacgcg
      121 gatttggatg cagaaaagga ccatctcgcg caccatttgg tatcgccaga gcccaaatgg
      181 cttatggtaa actcggctgg atgccggtca ctgactcaca aactgagcac gtcagcgaag
      241 tcgaagcagc tttgctcgac gccgagcttt tcatgaagta ccaggccccg gaacgcgcca
      301 taaaacgcct ccggaccgcc ctcgaaatca gcggccgatc catcaaactg cgcgagcgct
      361 tgcgcgaaat ctgcgccagt tacaaacaaa acgaggaggc cgcccgccag tgtctcgccc
      421 tcgccagcct ctacatcgag cgcgaagact ttgacactgc tcacgatcgc ctgctcgaag
      481 ccaaacagtt cgatggtcgg ataaacattg cacgcggtct tgaggcgatt cgccgcgcgc
      541 gccggcccga cctgatgccc gtaccagcac ctgaggccaa tttcgagcgc cggcacttca
      601 cgctcgccgg cgatctcagc acgatcagtg tcttcgacgc gattcaagtt attgaaaatg
      661 cgagactaaa tggcgtactc actctcagcg gtgaaaagca ccagggcgac gtgaagttca
      721 acgagggacg aatcgtcgat gcagcgtgcg gcacgaacgt cggcgatccg gcatttcagc
      781 aagtagttga aatcacgagc ggcacgtttg aatttcaaaa gaccgcggaa cagtttcccg
      841 ttcgcatcca ggcagtcagc aacacaaacc tcatcctcga cacgctccga atgctggacg
      901 agcgtaacca gtaagactaa tcaatcagaa cacgcgtgtt gagcgcttcg gctttttcga
      961 tggccgtgag gagtcgcaga aactggctgt agtttcgact ccgggacgtg agccgcgcga
     1021 cttccgactg gctcagctcg ctgcgcaaaa cgagatcgag aaaatcgtag tagtcggccg
     1081 cggcttcgaa aggaagctcg cgcgtgtagg tggtcgtctc gctgtcttcc gaccggaatc
     1141 cggcgagtag atcgaagatg ttgtcgctgg tcaggcgtgc ggcttcacgc gtgagcacaa
     1201 tcggcacgcg cagctcgaag aaccgcaaac catcttcagt aacaacgcgt cgcagttcct
     1261 tcgtcgcctc accatcaaac ggcgactccg gatcgaccaa aattcgaagc agtcgtggtt
     1321 gatcttcctt atcgagcgtg tcgagtgcga gttgtacgcg atccgcgaga aacttttcaa
     1381 actgcgcttc gaccgggtag ttgatccggc cgagacagcc cacggattcg ccgtctccag
     1441 cggtgtcgaa cggcaggcag gccgggcagt gtgtgcagat gatggtcgcc tcctgcatgc
     1501 gctcgatttc ggcgctcgcg ttttcaaccc attgcagctt gtcgatggaa ggcgcgctgg
     1561 cgtcaccggt ggtgagcttt gcgtgcaggc tgctcaggcg tccccactcg cgcaagtggc
     1621 gctcggaata gttggcgcgc agggggcaag tgtattgcag tgcaaattcc agcgacatgc
     1681 tggaagtata ggtcagatat gtcctatcgg tcatatttcg cctttgtgat aagctttctg
     1741 cattcccgcg cagaagtgcc gcttagaaag gtctagccca tggcagtcag tcaggaactc
     1801 ttggaaatac ttgcgtgtcc ggcttgcaag gcgaaggtcg agctcaagcc cgatgggaac
     1861 gcgctgaagt gtgttgagtg caaacgcgtc tatcccatcc gcgacgacat tcccgtaatg
     1921 cttatcgacg aggccaccat cgaagagtga tgcgatgaac agcctgcgcg acatcgttga
     1981 gctctctccc cagctacgcg tcatcgaaac acaggcaaac tacggatcgg aggcgcaagc
     2041 cctcgcgccg cctcgctggg actggtctgc agtgcgcaag gtgttgctgg tccgtttgcg
     2101 atctatcggc gacacggttt tggccacgcc ttcgctttac tcattgaagc gttttcttcc
     2161 gaacgtcaag gttgacattc tggtcgagga ttgggtggcc ccgctgctaa acaatcatcc
     2221 gcacgtcgac aacgtgatcg tgttggaacg cggcgggttc atgacgcggg cgcgtgtggc
     2281 acgtgagcta cgcgcggcga attacgatgt tgtttacaac ctgcatggcg gaactacagc
     2341 gacgtttctg acgcgcgcga ccggtgcgcg ttatcgcgtc ggctttaaga cgtatcaata
     2401 cgcgcaactt cacaaccac
//
LOCUS       DASUEX010000563         3044 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_780979, whole genome shotgun sequence.
ACCESSION   DASUEX010000563 DASUEX010000000
VERSION     DASUEX010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3044
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_780979"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="VFV61_09415"
     CDS             complement(<1..407)
                     /locus_tag="VFV61_09415"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HEX4900739.1"
                     /translation="MERIWTVGHSTRSGEEFNHILSVHKIAVLVDVRSFPGSRRYPQF
                     NKEHLRQFLAAAGVQYHHSPALGGRRRPNPNSKNMAWKNASFRAYADHLDSAEFKQGI
                     KELLEVARKDRTAIMCAEALWWRCHRSLIADYL"
     gene            complement(566..1582)
                     /gene="ligD"
                     /locus_tag="VFV61_09420"
     CDS             complement(566..1582)
                     /gene="ligD"
                     /locus_tag="VFV61_09420"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEX4900740.1"
                     /translation="MPKATKIPTRDFHPAKVQRASSPLPRTKAKEEPIKISKREVGVE
                     LNVGGRVVKLTNLRKPFWPELGITKRDLIQYYADVAPVLLPHLRDRAMVMKRYPNGAA
                     GDFFFMKRAPTPRPEWIELCSIEHSSGNIIDFPMIQDLASLLWVINLGCIDLNQWYAC
                     CDDVDRPDYVHFDLDPVPGATFEKVVEAALLIRTALESLKIPCFAKTTGSKGIHVYVP
                     IVRGPTQKQVWTFAKEFANAVAAQAPKLLTTIYVVAKRPRGRVLVDYNQNAWGRTLAS
                     IYSVRPRPRASVSTPVKWSELERGVKIEDFRIDNVPARIKRLGDLWQPLLEKRGRFKL
                     EAFL"
     gene            complement(1687..1947)
                     /locus_tag="VFV61_09425"
     CDS             complement(1687..1947)
                     /locus_tag="VFV61_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900741.1"
                     /translation="MAIGWGKSFEDQVEEGNQRLSQGERAPRLTQEERVRAQRLESLQ
                     LSRSRVLEQLDRSRHAAHRESLLKGLQVIEQEIENLTSGADD"
     gene            complement(1981..>3044)
                     /locus_tag="VFV61_09430"
     CDS             complement(1981..>3044)
                     /locus_tag="VFV61_09430"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEX4900742.1"
                     /translation="HFSNGFVEILAAEQTPESPQWFQGTADAVRQVLPHIDDWGIDTL
                     LILSGDHLYRMDYRQFLARHYESNADVTVSVIPSDPIDASEFGLLKTDASGRIVEFKE
                     KPKGAELESMRVDTTTLGLSPAEAQARPFLASMGIYVFKYDLLERLLAEETSWVDFGK
                     HVIPGAIQRANVQAFVFDGYWGDIGTISAFYKANLDLTSKIPKFNLFDAEAPVYTRAR
                     YLPPSKIEESEIRDSILSEGCIIGGARIANSVIGLRSRISKGVQMDASFMMGADFYQT
                     LEEMHLDVASGTPRVGVGENTIIKRAIIDKNARIGSNVRLVNEAGIANYDGEGGAYFI
                     RDSIIIVPKNAVVKDGIIV"
BASE COUNT          692 a          890 c          757 g          705 t
ORIGIN      
        1 tttaagtaat cggcaatcag actccgatga cagcgccacc acaaggcttc cgcacacata
       61 atcgccgtcc gatctttccg cgcgacctct agaagttcct taattccctg cttgaattca
      121 gcgctatcca gatgatctgc atacgctcga aaagaggcat tcttccaggc catgtttttt
      181 gaattcgggt tcgggcgacg cctgccacct aaggcaggtg aatggtggta ctgcactccg
      241 gcggccgcga gaaactgtcg caagtgttct ttgttgaatt gaggatagcg ccgtgagccg
      301 ggaaaactgc gaacgtcaac cagcaccgcg atcttatgca cgctcagaat gtggttgaac
      361 tcttcccccg accgtgttga atggccaacc gtccaaatgc gttccacaag agactctctc
      421 gatcctttct gctggctccc cgccatctca aatctctcgc actcatcaag gcgagctaac
      481 tgagaacttc aagtgtctgg ttgccgacct ctgaggtact acgtcaaaag ctgaaaggcc
      541 aaagaccaaa gacccaaggc ccgggttaaa gaaacgcttc aagtttgaag cggcctcgtt
      601 tctccaggag cggctgccag aggtccccca gacgcttgat tcgcgcgggc acattgtcga
      661 tgcgaaaatc ttcaatcttc acgccgcgct ccagctcact ccatttcact ggcgtcgaaa
      721 cagatgcccg tggtcgcggc cgcaccgaat agatcgacgc cagggttcta ccccaggcgt
      781 tctgattgta atcaacgagc acccttcccc gcggccgctt cgcgacaacg taaatggtgg
      841 tcagcaattt cggtgcttgc gctgccaccg cgttggcaaa ctccttcgca aaagtccaca
      901 cctgcttctg cgtcggtccg cgcacaatag gcacgtacac atgaatcccc tttgatcctg
      961 tcgtcttcgc aaaacaagga atcttcagcg actcgagcgc cgtcctgatc aacaacgccg
     1021 cttcaaccac cttctcaaaa gtggcaccgg gcaccggatc cagatcgaag tgcacgtagt
     1081 ctggccggtc cacatcgtca cagcacgcgt accattggtt caggtcgatg cagccgagat
     1141 tgataaccca cagcagcgag gccaaatcct gaatcatcgg aaaatcaatg atgtttcccg
     1201 atgagtgttc aatgctacag agttcaatcc actcaggacg cggggttggt gcgcgcttca
     1261 tgaagaagaa gtcgccggcc gcgccattgg gatagcgctt catcaccatt gcccgatcgc
     1321 gtaggtgcgg caggagtacc ggggcaacat ccgcgtaata ctggattaga tcgcgttttg
     1381 tgattcccag ctccggccaa aacggtttcc gcagatttgt gagcttgacg actcgaccgc
     1441 ccacattgag ctctacaccc acctcccttt tggagatctt gatcggttct tccttggctt
     1501 tcgttctggg taagggcgac gaggcgcgtt gtactttggc cggatgaaaa tcacgagtag
     1561 ggatcttagt ggccttgggc atgcttaaag tgattcctta actccagcct tgtttctgtt
     1621 ctaatagcta aggccaaata tcgaatcgga tagttttcta aagtaagacc tgagatggag
     1681 acttactcag tcgtccgccc cggaagtcag gttctcgatc tcctgctcaa ttacttgtaa
     1741 ccctttcagc aagctttccc ggtgagcggc gtggcgcgaa cgatccagtt gttcaagaac
     1801 tcgcgaacgc gaaagctgca gcgactccaa tcgttgcgca cgcacgcgct cttcctgggt
     1861 gagtcgcggc gcccgctcgc cctgcgatag gcgctgattc ccttcctcaa cctgatcttc
     1921 gaaagacttt ccccaaccaa ttgccactgc ctacctccgc cgtaaactgc gcgccggcca
     1981 ttagacaatt atgccgtcct tcaccaccgc attcttaggc acaatgataa tgctatcgcg
     2041 aataaaataa gcgccgcctt caccgtcgta gttggcaatg cctgcttcat tgacgagcct
     2101 cacattgctc ccaatgcgcg cgttcttatc gataatcgca cgcttgatga tggtgttctc
     2161 gccaacgccg acgcgtggag tgccgctcgc gacatcgagg tgcatctcct cgagtgtttg
     2221 atagaagtct gcgcccatca tgaacgaagc gtccatctgc acgcccttgg aaatgcgact
     2281 gcgcaaacca atcacggagt tggcgatgcg cgcccctccg atgatgcaac cctcgctcag
     2341 gatcgagtcg cgaatttccg attcctctat tttcgacggt ggtaggtaac gagcacgggt
     2401 gtataccgga gcctccgcat cgaacaaatt gaacttaggg atttttgaag tcagatccag
     2461 gttcgccttg tagaaagcac tgatagttcc gatgtcgccc cagtaaccat cgaaaacgaa
     2521 tgcctgcacg ttggctcgtt gaatcgcacc cgggatcaca tgttttccaa aatcgaccca
     2581 actcgtttct tctgcgagta gccgttccaa caggtcgtat ttgaagacgt agattcccat
     2641 cgaggccagg aatggacgag cttgggcctc ggcagggctt agtcctaatg tcgtagtatc
     2701 cacgcgcatg ctttccagct cagcaccctt cggcttttcc ttgaactcaa cgatgcgacc
     2761 actcgcatca gttttgagca ggccaaactc gctagcatca atcggatcgg aagggatgac
     2821 ggagacagtt acatccgcat tactttcata atgtcgtgcg agaaactgcc ggtagtccat
     2881 gcgatagaga tgatcgcccg acaatatcag gagcgtgtcg atgccccagt cgtcgatgtg
     2941 cggcaagacc tggcgcactg catcggcggt gccctgaaac cattgcggac tttcaggcgt
     3001 ctgttccgca gctaaaatct caacaaagcc gttggaaaag tgcg
//
LOCUS       DASUEX010000564         2542 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_910428, whole genome shotgun sequence.
ACCESSION   DASUEX010000564 DASUEX010000000
VERSION     DASUEX010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_910428"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1115
                     /locus_tag="VFV61_09435"
     CDS             <1..1115
                     /locus_tag="VFV61_09435"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX4900743.1"
                     /translation="LTAGRSLSMAPRTVAAAFAVSVRDSENEDLRGINMLQLARSTDL
                     KKVEKELAATPGIEYVHRVPRRWITARPRPNDPLLPRQWGLDSIGWFDPEPLPDASAI
                     KVAVLDTGVDITHRDLENVVQSYVHEGTSGEDVVGHGTHVAGIIAAEINNNVGITGIC
                     ECDLNVWKIFTDEPDPLDGEYYVDEVMYQRALNAVRNSGMRVLNLSIGGGSPSQTERM
                     LFRRLIDAGVTVVAAMGNEFNEGNPIEYPSAYVNVIAVGAVGRTKRRASFSNTGAHIS
                     IAAPGKAIVSTLPLRASAARTSQETRYASWDGTSMATPHVTAAAALVLAQKPDLTPAQ
                     VRRRLTSTATKLTAMGTKNKTNEFGFGLLNIERAVS"
     gene            1131..1403
                     /locus_tag="VFV61_09440"
     CDS             1131..1403
                     /locus_tag="VFV61_09440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900744.1"
                     /translation="MSGKEEIKQPLRLNVKLAPRVKPGNFKKVLSGTTGLRSVIQTFP
                     DERDEELSHLFIVEVEPDRATQALKELKDNPAVEYVERTAKRKLIR"
     gene            complement(1475..1954)
                     /locus_tag="VFV61_09445"
     CDS             complement(1475..1954)
                     /locus_tag="VFV61_09445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900745.1"
                     /translation="MTTKVLSLLLIAAIFSTYSPKSFAQGATTSWAGVQALAADERLI
                     VKQKDGKTVEGRMIEASETNLTMSRNGKVLNVARSDIAQLYHSTGKAAKGKWSAIGAG
                     VGAGAGFGIGAAKYSPHVDDSEIYMGFGLVLGAGIGALTGMAIGASKRHRTLIYQAP"
     gene            2270..>2542
                     /locus_tag="VFV61_09450"
     CDS             2270..>2542
                     /locus_tag="VFV61_09450"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX4900746.1"
                     /translation="MDRHREMRKKTESREPDVYERLCRARQFIDLCYDLPLNLDQISS
                     QACFSRYHFLRLFREAFDKTPHQYLVERRIEKAKELLSANELRVTDI"
BASE COUNT          596 a          683 c          698 g          565 t
ORIGIN      
        1 gcctgaccgc aggtcgttcc ctatcgatgg cgccgagaac ggtcgcggca gcctttgctg
       61 tcagcgtgcg cgactccgaa aacgaagacc tgcgcggcat taacatgctc caactcgcgc
      121 ggagcaccga cctgaaaaag gtcgaaaaag agctggcggc aacgccggga attgagtacg
      181 tccaccgcgt accacggcgc tggatcacag cgcgtccccg tcccaatgat cctttgctgc
      241 caaggcagtg gggactcgat tcgattggct ggttcgatcc tgaaccgctg cctgatgctt
      301 cagcgatcaa ggtcgctgtg ctcgacaccg gtgtagatat cacacaccgt gacctggaga
      361 atgtggtcca gtcttatgtt cacgaaggca ccagcggtga agatgtagtg ggccatggga
      421 cgcacgtagc gggaataatt gctgctgaga tcaacaataa cgtcggcata accggcatct
      481 gcgaatgtga tctcaatgtc tggaagatct tcactgatga gcctgatccc ctggatggtg
      541 agtattacgt tgacgaggtc atgtatcagc gcgcgctcaa cgccgtccgc aattcgggaa
      601 tgcgtgtgct gaatctcagt atcggtggag ggagtcccag ccagaccgag cggatgcttt
      661 ttcggcggct gattgatgct ggtgtgacgg tcgtcgccgc tatgggtaat gaattcaacg
      721 aaggtaaccc gattgagtat ccgagcgctt acgtgaacgt gattgcggta ggtgcggtcg
      781 gaagaaccaa gcggcgagct tcattttcaa atactggtgc ccacatcagc attgcagcgc
      841 cggggaaggc catcgtttcc accttgccgt tgcgagcttc tgcggcgcgg acctctcagg
      901 aaaccaggta tgcatcctgg gatggcactt ccatggccac tccgcatgtc acagccgcag
      961 cagcactagt cctcgcacag aagccagacc tcacgccggc acaggttcgt agacggttga
     1021 cttcgactgc aaccaagctc accgcgatgg gaacaaagaa taagaccaac gaatttgggt
     1081 ttggtttact taacatcgaa cgggcagtat cctgactttt gaggatcagg atgagcggta
     1141 aagaggaaat taaacaaccc ttgcgcttga acgtgaagct tgctccgcgc gtcaagccgg
     1201 gaaacttcaa gaaggtatta tcgggtacta cagggctacg ttcggtgata cagaccttcc
     1261 cggatgaaag ggacgaggaa ctgtctcatc tgttcatcgt tgaagtggag cccgaccgtg
     1321 ctacgcaagc gttgaaagag ttgaaggaca atcccgcggt tgaatatgtt gagaggactg
     1381 cgaagagaaa gttgattagg tagttaggtg ggcagtggat agcgggcagg ccaattggca
     1441 attgcccagg tccactaccc acttcctttc tccctcacgg cgcctgatat attaacgtcc
     1501 ggtgccgctt gctcgctcca attgccattc ccgtcaaagc gccgattccg gcgccaagca
     1561 caaggccaaa ccccatatag atctcgctgt catccacatg cggactgtac tttgctgcgc
     1621 caatgccaaa gccggcgcct gctccgacgc ccgctccgat tgctgaccac ttgcctttag
     1681 cggctttgcc cgttgaatga tagagctgtg cgatgtcgct ccgagcaacg tttaggactt
     1741 tgccattccg agacatcgtc aaattcgttt ctgaagcttc aatcatcctg ccttcgacag
     1801 tctttccgtc cttctgcttc acaattaatc gctcatcagc tgccagcgcc tgcactcccg
     1861 cccacgacgt agtcgcgccc tgggcaaaac tcttgggcga gtaggtggag aagatcgctg
     1921 cgataagcag cagtgagaga actttggttg tcatgttcgc tcctgatcca aataaaaatt
     1981 taggtagaac ccagagttca ggtgggcaac cagtgttccg ctcgttacgc ggttcagccc
     2041 ggtaggagtt gacgtgtatt caactggcag gtgaaaactt cacctgcatg gcggactgtc
     2101 gtgccgcagg aaattgctga caggtgaagg tttcagactt cggatgcagt agaagagttg
     2161 gctgacctca tagcagtcat acagcccacc gggtaacacg gcacctggca aaacccttga
     2221 cccagtcggc cgctttacgc taagagtgat tgccaattcg gtatctgcta tggacaggca
     2281 tagagagatg agaaagaaga ctgaaagcag agagccagac gtctatgaac gactctgccg
     2341 agcgcgccag tttatcgatc tctgctacga cctgccgtta aatctggatc agatctccag
     2401 tcaggcttgc ttttcgcgtt accactttct gcggctgttc cgcgaagctt tcgacaaaac
     2461 acctcaccag tacctggttg agcgacgtat tgaaaaagcc aaagagttgc tttctgcaaa
     2521 cgaactgcga gtcaccgaca tc
//
LOCUS       DASUEX010000565         4073 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_187926, whole genome shotgun sequence.
ACCESSION   DASUEX010000565 DASUEX010000000
VERSION     DASUEX010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4073
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187926"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..738
                     /locus_tag="VFV61_09455"
     CDS             <1..738
                     /locus_tag="VFV61_09455"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX4900747.1"
                     /translation="FPEVSQSERERLLRGCFDNLGRMLGEFSQLSRITPDELRQIIDC
                     EGLDHLRSAQSRGRGVILFTGHLGAWEFTSFALSAFGHPFSFMVRRLDNDLIERMIDK
                     VRQRFGNRTLDKNSVSRQALRELNDGGTLGLLPDVNMLAREGVFVDFFGTPASTTSML
                     AKLALRTGAAVVPIYAPWDEQQQRFLLQLEPPLLVERTGDEAEDVRRLTAAFMASIEN
                     VVRRYPEQWLWIHKRWKTRPPGEPDLY"
     gene            787..1542
                     /locus_tag="VFV61_09460"
     CDS             787..1542
                     /locus_tag="VFV61_09460"
                     /inference="COORDINATES: protein motif:HMM:NF015961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX4900748.1"
                     /translation="MQVSAESTATKVPARAGDVERAYKFADLSEYPFKKRLIIRLADL
                     AFYSLINLIGRTVRFEVTGWENHEQVVSEGHLPIYVFWHERIFLTTYWWRNRRIVVLT
                     SKSFDGEYIARFIQRFGYGAVRGSSTRGGVGAVVEMVRLMRSGCTTAFTIDGPKGPPR
                     VAKLGAVLLAKKSGHPILPVTMALERRWIAPTWDSFQVPKPFSRACVFVAPPIYVPGE
                     ADEAELAAKNELLQSTLDELTRRGEEWRARLKK"
     gene            1667..2569
                     /gene="era"
                     /locus_tag="VFV61_09465"
     CDS             1667..2569
                     /gene="era"
                     /locus_tag="VFV61_09465"
                     /inference="COORDINATES: protein motif:HMM:NF000908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEX4900749.1"
                     /translation="MADSDKTFHSGYVALIGRPNAGKSTLLNQLVGQKIAAVSDKPQT
                     TRFRIQGVINQPAGQIVLVDTPGIHKPGYELNRRMMSVVQDALLGVDLVCLIRDASVS
                     TGNGDRFVLELVQRSGKPALLLLNKTDKLKDKAALLPLIEWYRSEYEWREVVPISALR
                     GRMIDVLLEQMIKHLPPGEPLFAADELTDQSLRSLAAEIVREKILQTTADEIPYVTAV
                     VTEKWDEERPDFTRIYCAIFVERASQKKIIIGKGAARLKEIGIAARQEIESLIGHRCH
                     LELFVKVEAEWRNKQHLLDELGIG"
     gene            2653..3510
                     /locus_tag="VFV61_09470"
     CDS             2653..3510
                     /locus_tag="VFV61_09470"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEX4900750.1"
                     /translation="MNKHSQWYALDLAIEPTGSEAVQYALMEAGALGTEINDADLSVQ
                     IATGYFESLPEREHVRAELAAALRIYDLPSSAVRNMSLREVADEDWLGEWKKSWQPAA
                     VGDRFLVAPPWSEIDDPLGRIVIRIEPGRAFGTGTHETTRLCLATIEKHFAGESFLDV
                     GTGTGILAIAAAKLFPEARIEACDTDAEAVAIAIENARLNGVAESISFRVGSVSESTA
                     SAALVCANLTADVIVALLPTLVGATCGRLILSGILEEQLPQVIDALRAQGIVSPDITQ
                     DGEWVAVVV"
     gene            complement(3624..>4073)
                     /locus_tag="VFV61_09475"
     CDS             complement(3624..>4073)
                     /locus_tag="VFV61_09475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HEX4900751.1"
                     /translation="TELGVVRLVPVTTRLADIKLKDESDSLKRLSRWRRIALEAAKQS
                     GRALVPEVAMPILFSDLIGSQQQSDYRVLFSERNGESIDSLVPGAESNGSLTALIGSE
                     GGWTDAELEAARAAGWKVVTLGGRTLRAETAAIAVTALLQHRFGDLN"
BASE COUNT          894 a         1088 c         1156 g          935 t
ORIGIN      
        1 tttcccgaag ttagccaaag cgaaagagag agactgctgc gtggatgttt cgataacctt
       61 ggccggatgc tcggcgagtt cagtcagctt tcccgtataa ctcctgacga gttaagacaa
      121 atcatagact gcgaaggtct cgatcatttg cgttcagcgc agtctcgtgg tcgcggtgtg
      181 atcctcttta cgggccatct cggcgcttgg gagtttacga gctttgctct ttctgcattt
      241 gggcatcctt tcagttttat ggtgcggcgg ctcgacaacg acttgattga aagaatgatc
      301 gataaggtgc gacaacgttt tggaaatcgc acgctcgaca aaaacagtgt gtcgcggcag
      361 gcgttgcgcg agcttaatga cggcggcacg ctgggactgc tcccagacgt caacatgctc
      421 gcgcgtgaag gcgtgtttgt tgattttttt ggtacgccgg cctcaacgac atcgatgtta
      481 gctaaactcg cattgcggac gggggctgca gtggtgccga tctacgctcc ctgggacgag
      541 caacagcaac gttttctttt gcaacttgag ccgcctttac tggttgagcg aactggtgat
      601 gaagcagagg atgtgcgaag acttacggct gcattcatgg cgagtattga aaatgtcgtg
      661 cgacgatatc cggagcaatg gctctggatt cataagcgtt ggaagacccg tccacccggc
      721 gagccggatc tgtattagag ggggaaggcg gaaggcggta acctgacaac cgaccactga
      781 caagttatgc aagtctcggc agaatcaact gctaccaaag ttcccgcgcg cgccggcgat
      841 gtggaacgcg cctacaaatt tgccgatctg tctgagtacc cgtttaagaa gcggctgatc
      901 atccggcttg ccgatctggc gttctactct ctcattaact tgattggacg cacggtcagg
      961 tttgaagtta ctggctggga gaatcatgag caggttgtta gcgaaggaca tttgcccatt
     1021 tatgtctttt ggcacgagcg catctttctg acgacttatt ggtggcgcaa tcgccggatc
     1081 gtggtactca cttccaagag tttcgacggc gaatacattg cccggttcat tcagcgcttc
     1141 ggttacggag ccgtacgtgg atcttcgacc cgcggcggag tgggtgcggt cgtggagatg
     1201 gtgcgcctaa tgcgcagtgg ctgcaccact gcctttacca tcgatggtcc aaagggaccg
     1261 ccacgggtcg ccaagctggg tgcggtgttg ctggcgaaga aatccggaca tccgatcctg
     1321 ccggtaacta tggcgctcga acgccgctgg attgcaccga cttgggacag ttttcaggtg
     1381 cccaagcctt tcagccgggc ctgtgtattt gtggcgcctc cgatttacgt cccgggcgaa
     1441 gctgacgaag cagagctggc cgcaaagaat gagctcctgc agtcgaccct ggacgaatta
     1501 accaggcgtg gagaagaatg gcgcgcgagg ttgaagaagt aacaggtaat gctcagtcag
     1561 gaaggtgggc tttcccccgc tgtgggttga ttagcactcg ttcctgactg gttactccgc
     1621 tttactccgg gcctgagcat tactcggagc tcacataaca tctgtcatgg ccgattccga
     1681 caaaacattt cacagcggtt acgtggctct aatcgggcga ccaaacgccg gcaagtcaac
     1741 cctgctcaat cagcttgtgg ggcagaagat tgctgccgtt tccgataagc cccaaaccac
     1801 ccgtttccgt atccagggtg tgatcaatca gccggccggc caaatcgttc tcgtcgatac
     1861 ccctgggatt cacaaacccg gttatgaact taaccggcgc atgatgtcgg ttgttcaaga
     1921 cgcgttatta ggtgtggatt tggtttgtct cattcgcgac gcctcggtgt cgaccggcaa
     1981 tggcgatcgt ttcgttttgg agttggtgca acgatcaggg aagcccgcgc tcttgttgct
     2041 gaacaagacc gacaagctaa aagacaaggc ggccctgctc cctttgatcg agtggtaccg
     2101 cagcgagtat gagtggcgcg aggttgttcc tatctccgcc ttgcgaggaa gaatgattga
     2161 tgtcctcctc gagcagatga tcaagcacct tccccccggt gaaccgttgt ttgctgctga
     2221 cgaacttact gaccagtcgc tgcgcagcct cgcggccgag attgtgcgcg agaagattct
     2281 gcaaactact gctgacgaaa tcccgtacgt caccgcggtc gttaccgaga aatgggatga
     2341 agaacggccc gacttcacgc gcatctactg cgccattttt gtcgagcgtg catcgcagaa
     2401 gaagatcatt attggaaaag gcgcggcgcg cttgaaagaa atcggaatcg cggcaaggca
     2461 agagatcgaa agtttgattg gacatcgttg ccacctcgag ctcttcgtga aagtcgaagc
     2521 agagtggcgc aacaaacaac acctgctcga cgaattggga attggttagt gagtgatagt
     2581 gcgccttcat aactcatcgg agcaggtgga tccgagcatc ctaactagcg tttccaaccc
     2641 aaccttgcgt taatgaataa gcactcccaa tggtatgcac ttgatcttgc cattgaacca
     2701 acaggcagcg aagccgttca gtacgccttg atggaagctg gcgctctcgg tacggagatc
     2761 aacgacgcgg acctctcggt gcaaatagca accggctact tcgaatccct tcccgagcgt
     2821 gagcatgtgc gcgcggagct tgccgcagca ttgcggattt acgatttgcc ctcttcagca
     2881 gtgcgaaaca tgagcctgcg cgaagtcgca gatgaagact ggctcggcga atggaaaaag
     2941 agctggcagc cggctgcggt aggtgatcgt ttcctggtcg caccaccctg gtctgagatt
     3001 gacgaccccc tgggaagaat cgtgattcgc atcgagccgg gtagggcttt tggcaccggc
     3061 actcatgaaa caacccgcct ttgcctggca actatcgaaa aacactttgc cggagagagt
     3121 ttcctcgacg taggcaccgg aaccggaatc ctggccatcg ccgcggcaaa gctctttccg
     3181 gaagcacgca ttgaggcgtg cgataccgac gcagaggctg tggcgatagc gattgagaat
     3241 gcgcgcttaa acggagttgc ggagagtatt tcgtttcgcg tgggcagtgt ttctgagtcg
     3301 acagcttcag cagcacttgt ctgcgccaat ctgacggcgg atgtaatcgt ggcgttgctg
     3361 cccacattgg ttggcgccac atgcggaaga ctaattctct ccggaatctt agaagagcaa
     3421 ctacctcaag ttattgacgc cttgcgcgct caggggatcg tttccccgga tatcactcaa
     3481 gatggcgagt gggtggctgt ggtcgtgtga aggttttatt acgctcagtc cggaagggcg
     3541 gtttaccccc gctggttgag tggacttcaa tcttagcggg ggcaagcccg ccttccagac
     3601 tgcgcctaaa cgtaccgacg ccattaattc aaatccccaa agcgatgctg cagcagtgcc
     3661 gtaacagcga tcgctgccgt ctctgcgcgc aaagttcgtc cccctagcgt caccactttc
     3721 catcccgctg ctcgagcggc ttcaagctct gcatccgtcc acccgccttc ggagccgatc
     3781 agcgccgtca gggaaccgtt cgactctgct cctgggacca aagaatcgat cgactcgcca
     3841 ttacgttccg aaaacaaaac tctgtaatca ctctgctgtt gcgagccgat caagtcagaa
     3901 aacaaaatcg gcatggcgac ctcaggaaca agagcccggc ctgattgctt ggcagcttcg
     3961 agggcgatgc gacgccaacg actcagtcgc tttaggctat ctgattcgtc tttaagtttg
     4021 atgtccgcca acctggtcgt cactggaaca aggcgcacta ctcccagctc cgt
//
LOCUS       DASUEX010000566         2164 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1599672, whole genome shotgun sequence.
ACCESSION   DASUEX010000566 DASUEX010000000
VERSION     DASUEX010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1599672"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2164
                     /locus_tag="VFV61_09480"
     CDS             <1..>2164
                     /locus_tag="VFV61_09480"
                     /inference="COORDINATES: protein motif:HMM:NF020083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900752.1"
                     /translation="KRFCRKFNFELQAAATEDAIILSLGPTHSFPLEDVFHYLSSKTV
                     RDLLCQALLDAPMWNIRWRWNVTRALAVLRRRGGKKIPPQLQRMDAEDLLTALFPDQV
                     ACFENLTGQREIPAHPLVSQTVRDCLEEAMDVDSLIALLAAIERNEKNLIARDLLEPS
                     PLAAEILNARPYAYLDDAPLEERRTRAVYQRRWLDPEKAADIGRLDQSAIDRVREEVW
                     PEPRNADELHDALVELGFITEAEGLLGHFAVAEDIASDEATDATPDWKSLLELLASER
                     RAAVLEVHGIAGVSPATARMTATEETVEVAHGGHLNPSDDEGAHRGTPLHLWVAAERL
                     PQLLAVFPEVTLTPPISAPASLTQATWTREDALVELVRGRLEGLGPVTAKSLAVSTGL
                     TINDIEATLLKLEAEGFVLRGRFTPGSEETEWCARRLLARIHSYTLNRLRQEIEPVSS
                     ADFVRFLLSWQKVAPDQQMEGPQSLLALIEQLEGFEAPAAAWEGEIFPSRLLEYNPAW
                     LDALCLSGEVVWARLTPAISAKNGAEKPRGSGPVRSTPIALLRRRDFALWNSVFPRPT
                     VAELTFSTTTQVVYDYLKTRGASFFNDVVEGSKLLRSQVEESLAELVALGLVVSDSFT
                     GLRALLTPSHRKTNAAARRKHRQPVYDMATAGRWSILERGSGDGVDPEAAEKIARIFL
                     KRYGVVFKRLLEREGVTIPWRVLLRTYHRLEARGEIRGG"
BASE COUNT          433 a          585 c          671 g          475 t
ORIGIN      
        1 aaacgtttct gccgcaagtt taattttgaa ttgcaggcgg ccgccaccga agatgccatc
       61 attctatcgc tgggtcctac gcatagtttt ccactcgagg acgttttcca ttatctgagt
      121 tcgaagaccg tccgcgatct attgtgtcag gcgttgctgg atgcgccgat gtggaacatc
      181 cgttggcgct ggaatgtcac gcgggcgctg gctgtcttgc gtcgccgcgg cggcaagaaa
      241 attccgccgc agttgcaaag aatggatgcc gaagacttgt tgacggccct ctttccggat
      301 caagtggcct gtttcgaaaa ccttaccggt cagcgcgaaa ttcccgcaca ccccttagtg
      361 agccaaacag tgcgcgactg tctcgaagaa gcaatggacg tggacagcct gatcgctctg
      421 ctggcagcaa tcgaaagaaa cgaaaagaac ctgattgcgc gagatttgct tgaaccatca
      481 ccgctcgcgg cggagattct gaacgcgcga ccctacgctt acctcgacga cgcgcctctg
      541 gaagagcggc gcacccgcgc ggtgtatcaa cggcgctggc tcgatccgga aaaagctgct
      601 gacattggga gactggatca gtcagcaatc gatcgcgtgc gtgaagaggt gtggccggaa
      661 ccgagaaacg ctgatgagtt acacgacgct ctggttgaac tgggatttat taccgaggct
      721 gaggggttgt taggtcactt cgcagtcgcc gaagacattg cttctgatga ggcgaccgat
      781 gccacgccgg actggaaatc attgttggag ctgttggcga gtgagcggcg cgccgctgtg
      841 cttgaggttc atgggattgc cggcgtctcg cccgccacgg cgaggatgac cgcgactgaa
      901 gagactgtag aggtggccca cggtggccac ctcaatccga gcgacgatga gggtgcccac
      961 agagggacac ccctacatct ctgggtcgct gcggaacgac taccgcaatt gttggcggta
     1021 tttcccgaag tcacattaac tccgccgatc tctgctcccg cgtcgttgac tcaagccacc
     1081 tggacacgcg aagacgctct ggttgaactc gtgcgcggta gattggaagg acttggtccg
     1141 gtcacagcga aaagcttagc cgtgtccaca gggctgacaa tcaacgacat cgaggcgacg
     1201 cttctaaaac tcgaagccga aggttttgtg ctgcgggggc ggttcactcc gggcagcgaa
     1261 gagactgagt ggtgtgcgcg acgtttgttg gcgcggattc acagttacac gctgaatcgt
     1321 ttgcgccagg agattgagcc ggtctccagt gccgattttg tgcgctttct tttgtcgtgg
     1381 cagaaggtcg cgccggatca gcagatggaa ggaccgcaga gtttgctggc ccttattgaa
     1441 cagctcgagg gcttcgaagc tccggcggct gcctgggaag gcgagatctt tcccagtcgc
     1501 ttgttggaat acaaccccgc ctggcttgac gccctatgtc tgtctggtga agttgtttgg
     1561 gcgcgcttga cgccagctat ttcggcgaag aacggcgcgg aaaagccgcg cggttccgga
     1621 cccgtgcgct caacgccgat tgcgctgctg cgtcgtagag acttcgcgct ttggaattcc
     1681 gtgtttccgc ggccgacagt tgcagaactc actttctcaa ccaccaccca ggtggtttac
     1741 gactatttga aaacgcgcgg agcatctttc tttaatgacg tcgttgaagg ctccaagctc
     1801 ttgcgcagtc aggttgaaga gtcgcttgcg gagttagtcg ccctgggact cgtggtctcc
     1861 gacagtttta ctgggttgcg cgctttgctg actccatctc atcgcaagac caacgccgcg
     1921 gcgcgtcgca agcaccggca acctgtttat gacatggcca cggcgggacg ctggtcgatt
     1981 ctagagcgcg gcagtgggga tggggttgat ccggaagcgg cagaaaagat cgcccgcatt
     2041 ttcttgaaac ggtatggcgt ggtcttcaag cgtttgctcg agcgggaagg cgtgacgatc
     2101 ccctggcgcg tgctgttacg gacttaccat cggctggaag cgcgcgggga gattcgcggc
     2161 ggca
//
LOCUS       DASUEX010000567         3267 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1599734, whole genome shotgun sequence.
ACCESSION   DASUEX010000567 DASUEX010000000
VERSION     DASUEX010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3267
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1599734"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..906
                     /locus_tag="VFV61_09485"
     CDS             <1..906
                     /locus_tag="VFV61_09485"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEX4900753.1"
                     /translation="KHPLSACPANTSKFDCDSVAELPGAPPQPWLVAKPGVAEGRVEK
                     QTIKSAIQKIDRPFSVYTPANYKEDGPPNALLILFDGEDLADDQYQVTTLNNLIAASR
                     IPPTVAVFVDNIPRRRLVDLVASDEFADFMAKELVPWIRSHYNVTKDSKQTVITGYSA
                     GGLASAYVALRHPEVFGNVLSQSGAFWWSFEHNGGVCGSRCPASGGRGGDGSRDVTTE
                     GNIMVKQFLASPKLPLRFYLAAGTLEFDRNGGGGEILEGTRHLRDVLLAKGYQVHYQQ
                     FVGGHDGLSWRGALADGLINLLGVH"
     gene            949..1899
                     /locus_tag="VFV61_09490"
     CDS             949..1899
                     /locus_tag="VFV61_09490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900754.1"
                     /translation="MKELFTRIALAALIALAFASLPALAQVTDDPVKVEMYKRFTDNR
                     VPKPEVAYQAAKDYLQKYSKDKDQYTDYLEKWTAAYDSDERKRNLPILINQKNFTEAY
                     RVGAEILANEPGYLRAEIDLGYAGYLAASNNNETHNADALAYARKAIRAIDSGKTPAD
                     WAPFKGKDDTLAYLNYAAGFLTLKVNPDETISALIRAAQYESEIRRTPSTFYFLAAAY
                     ESGPYKTMSAAYQAAYANKPETPQSKAALEKLNVVMDRIIDAYARAIATAGSNPNTEQ
                     SRKEWLAQMTNYYKFRHDGSDVGLIEFIATSIQKPLPPKP"
     gene            complement(1983..>3267)
                     /locus_tag="VFV61_09495"
     CDS             complement(1983..>3267)
                     /locus_tag="VFV61_09495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900755.1"
                     /translation="TRNNGDAVVRYDQLARRWLIVMPIFARGPARPDQPRVGVSGEPA
                     QLSPPGRPNQPGPAVQLVPPAQANPADPARAAPATKGTGPYSMCYAVSVSPDPLGKYY
                     RYEFLRPLFPDYPRPAVWPDGYYNPTSTSDDFIQKHACVAERNKMLKGLPATEQCFVI
                     DGVNFLNNADIDGQGVPPRGAPNIMMAAGGAQLKNVLEDNRVFFWKFHVDWKNPAKTN
                     IDGPHPIAVAHYSYLCGGQLTDCVPQPGTDRRLDAQGDKLMARLVYRRLKGRESIVAV
                     HSVGTSAGGGGVRWYEFRLDRNRNPQVYQQGTYAPDKFYRWMASPAMDRNGNIGIGYS
                     FGGTPNFPGQRFAARLANDPLGQLTLQETVLMEGEGVQNVMRWEDYTQTAVDPSDDCT
                     IWYVGDYIKKGETNYSSRIGAFRLPGCGGKETRKR"
BASE COUNT          761 a          891 c          887 g          728 t
ORIGIN      
        1 aaacatccct tgtcagcatg tccggcaaat accagcaagt tcgactgtga cagcgtagca
       61 gagttgcccg gagccccgcc gcaaccatgg cttgttgcca aacctggagt agcggaagga
      121 cgcgtcgaga aacaaaccat caagagcgcc attcagaaaa ttgaccgtcc cttttccgtt
      181 tacacgccgg ccaactacaa agaagatggt ccacctaatg cgttgttgat tttgtttgac
      241 ggagaagact tggcagacga tcaataccag gtaacaacgc taaacaatct cattgcagct
      301 tcccggattc cgccgaccgt cgccgtattt gttgacaaca ttcctcgtcg tcgtctggtg
      361 gatcttgtcg ccagtgatga gttcgcggat tttatggcaa aggaacttgt gccctggatt
      421 cgatctcatt acaacgtcac aaaggattcg aagcaaaccg ttatcactgg ctacagcgct
      481 ggggggcttg cctcagctta tgtcgctctg cgtcaccctg aagtctttgg caatgtgctc
      541 agccaatccg gcgccttctg gtggtcgttt gaacacaacg gtggcgtctg cggttcgcgc
      601 tgtcctgctt cgggcggacg cggtggtgac ggttctcgag acgttacgac cgaaggcaac
      661 atcatggtta agcaattcct cgcgagtccg aagctgccat tgcgtttcta tcttgcggcg
      721 ggaacacttg aattcgacag gaacggtggt ggaggcgaga ttctggaagg cacgcgtcac
      781 ttgcgtgatg tgttactggc aaaagggtat caggttcact accagcaatt cgttggcggc
      841 catgacggtt taagttggcg cggcgccctc gctgacggcc taatcaactt gctcggagtt
      901 cattgagttt tgatacgaaa actacgttgc catgaggaaa catttaatat gaaagagttg
      961 tttaccagaa tcgcactggc ggcactcatt gccttagctt ttgcgtcctt gccagcgctg
     1021 gcccaggtga ctgacgatcc ggttaaggtc gaaatgtaca agcggttcac ggacaaccga
     1081 gttccgaaac cggaagttgc gtatcaagca gccaaagact atctacagaa atatagtaag
     1141 gacaaagacc agtacaccga ttacctggaa aaatggaccg cggcttacga tagtgacgag
     1201 cggaagcgga atctgccaat tcttattaat cagaaaaact tcactgaagc atatcgtgtg
     1261 ggagccgaga tactcgccaa cgaaccaggc taccttcgcg cggagataga tcttggctac
     1321 gcaggttacc ttgctgcgtc aaataataac gagacgcaca acgctgacgc gctcgcctat
     1381 gctcgcaaag ccattcgagc aatagacagc ggcaagacgc cggctgactg ggcgcccttc
     1441 aaaggcaagg atgacacgct ggcctacctg aattacgcgg ctggtttctt aacgttgaaa
     1501 gtgaatcccg atgagacgat tagtgctttg ataagagcgg cgcaatacga aagcgagatt
     1561 cggaggacac cttcaacctt ctactttctg gctgcggcct acgaatctgg tccttacaag
     1621 acaatgtccg ctgcgtatca agccgcttac gcaaacaaac cagagacgcc gcaaagtaaa
     1681 gctgcactag agaaattgaa cgttgtcatg gatcggatca tcgacgcata tgcgcgtgcg
     1741 atcgcaaccg ctggaagcaa tcccaataca gagcagagtc ggaaggagtg gctggcgcag
     1801 atgacgaact actacaaatt tcgacatgat ggaagtgatg tcggccttat tgaattcatc
     1861 gccacctcca tccagaagcc gttgccacca aaaccatgat ctgctgagct ggggggtcag
     1921 gcttgcgatt ttgcgattct cacatcacac gtgatcgggt cgcaactata cgccgtcgct
     1981 acctatcgtt tccgtgtttc tttgccgcca cagccgggca ggcgaaaggc gccgatccgg
     2041 ctcgagtaat ttgtctcgcc ttttttgatg taatcaccga cgtaccagat ggtgcagtcg
     2101 tcgctcgggt ccacggccgt ctgcgtgtag tcttcccagc gcatcacgtt ttgcacgccc
     2161 tccccctcca tcaaaacagt ttcttgtaga gttaactggc ccagtggatc gttcgcgagt
     2221 cgcgccgcga aacgctggcc ggggaagttt ggagttccac caaacgaata gccgatgccg
     2281 atgttgccat tccggtccat cgctggactg gccatccaac gataaaactt atcgggagcg
     2341 taagtgcctt gctgataaac ctgcggatta cgattcctgt cgaggcgaaa ctcgtaccaa
     2401 cgcactcctc ctcctccagc cgacgtgccg accgagtgaa ctgccacgat cgattctctg
     2461 cctttaagcc ttcgatagac cagccgcgcc atgagcttgt cgccctgagc gtcaaggcgc
     2521 cggtctgttc ccggttgcgg cacacagtca gtcagctgtc ccccgcacag gtatgagtag
     2581 tgggccacag cgatcggatg tggcccatcg atgtttgtct tggcgggatt tttccagtcc
     2641 acatgaaact tccagaagaa cacacgatta tcttcgagga cgttttttag ttgcgccccg
     2701 cctgccgcca tcatgatgtt tggcgcgccg cggggcggca ctccctgacc atcgatgtca
     2761 gcattgttca gaaaattcac gccgtcgata acaaaacatt gttcggtggc gggcaaaccc
     2821 ttgagcatct tgtttcgttc agcaacgcag gcatgcttct gaatgaagtc atcgctggtg
     2881 ctggttgggt tgtagtaccc gtcaggccaa actgcgggac gcggataatc cgggaatagc
     2941 ggccgcagaa actcatagcg gtaatacttc ccgaggggat ccgggctcac actcaccgcg
     3001 tagcacatcg agtaaggtcc ggtccctttc gtcgccggcg cggcgcgagc gggatcagca
     3061 ggatttgctt gcgcaggtgg aacaagttgg acggctggtc ccggctgatt ggggcgaccc
     3121 ggcgggctaa gttgggcggg ctcgcccgag acacccacac gcggctggtc aggtcgcgct
     3181 ggaccacgag caaagatcgg cataacgatg agccaacgcc tggccaactg atcgtagcga
     3241 acaacagcgt cgccattgtt ccgagtc
//
LOCUS       DASUEX010000568         2848 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1687188, whole genome shotgun sequence.
ACCESSION   DASUEX010000568 DASUEX010000000
VERSION     DASUEX010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2848
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1687188"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..981)
                     /locus_tag="VFV61_09500"
     CDS             complement(<1..981)
                     /locus_tag="VFV61_09500"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX4900756.1"
                     /translation="MQLPTLAKRVWLLLFFAIIVVSYLYGLGYLPLVGPDEPRYAQVA
                     REMYQRADLITPTLGGHTWFEKPALLYWFMMAAFRLCGSTETAVRLGPAVFGLLTVLA
                     VFWVGRKVARTSGVAELCGAGTWSALVTASMLGMIVFSRAASFDMIVTMTITWALSFF
                     FVHEVESDEARRRWLLMGFYFFVGLSLLAKGLIGIVMPFGVIVIYQLMRRAYLSRWFL
                     RSLLWGIPLAAAVAALWYGPVIARHGWPFINEFVVQHHFARYFSNKYRHPGPVYFYLL
                     VLIPLTLPWTVLVVDALSQIRSWKWRGTDPLDKLRVFSLAWLLLPLALFSF"
     gene            1105..2103
                     /locus_tag="VFV61_09505"
     CDS             1105..2103
                     /locus_tag="VFV61_09505"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX4900757.1"
                     /translation="MSLRTAVIGVGHLGRQHARLHGSLAAEGLSEFVAVCDQNAETAR
                     TIAAERSVSWNTDWRELLGQVDAVSLAVPTVSHCEIACGLLEKGIHVLVEKPIARTLA
                     EADQMIAAADKGNALLQVGHLERFNPALVALRPHVRNPVYFEIHRVGEFTARSLDIDV
                     VLDLMIHDLDIVQWLVGEEVEVTELHAVGIPILTNKVDAANARLEFSSGAVANITASR
                     VGTEKIRKMRFFQPHDYVAVDYTTKRASISGLAPPTAEGLWPGVQVRNLEVVDVEPLR
                     MEIAGFFTAARDGLPSPVSGRDGRNALSLALRVLESIHEHGRRSNVAALSAQTPRR"
     gene            2206..>2848
                     /locus_tag="VFV61_09510"
     CDS             2206..>2848
                     /locus_tag="VFV61_09510"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX4900758.1"
                     /translation="MSDAAPTREIHISATLSERIAAGDFPSAVYLIAEKGQPVFADAL
                     GHAVVDPYRITASLNTIYDLASLTKPLITGMLCAQLVEKGELTLDSAVANYLPEFDRT
                     DRQMITVRELLTHTSGLPAWRPLYILAEGERERALGVIVNEALEYKPGTRVVYSDLGF
                     IVLGFLLERLIGRGLDELARTEIIQPLKLQHTFFNPEMALQTGIAACETGNAYE"
BASE COUNT          709 a          796 c          797 g          546 t
ORIGIN      
        1 gaacgaaaag agcgcgagcg gtagcagcaa ccaagcaagg ctgaagactc tcaacttgtc
       61 caaaggatcc gttccccgcc acttccatga tcgaatctgc gaaagtgcat caactaccaa
      121 cacggtccac ggcagcgtaa ggggaattag aacgagaaga tagaaataga ccggcccagg
      181 atgccgatac ttgtttgaga agtaacgcgc gaaatgatgt tgcacaacga actcgttaat
      241 gaagggccag ccatggcgcg cgattacagg cccgtaccaa agggccgcga cggcagcggc
      301 aagcggtatc ccccacagca gcgaccgcaa gaaccaccgc gagagatacg cccggcgcat
      361 cagttggtag atgacaatta cgccaaatgg catcacgatc ccgattaggc ctttagcgag
      421 cagcgacagg ccaacaaaga agtagaaccc catcaatagc cagcgccgcc tcgcttcgtc
      481 gctttctact tcgtgaacga aaaaaaagct taacgcccag gtgattgtca tggtcacgat
      541 catgtcgaaa ctggcggcgc gggaaaaaac gatcatgcct agcatcgaag cggtaaccag
      601 ggcgctccag gtgccagcgc cgcacaactc ggcaacacca cttgtacgag caacctttcg
      661 accgacccaa aaaaccgcca gcaccgttaa taagccaaaa actgccggac cgagtcgcac
      721 agcagtttcc gtgctgccgc acagtctgaa agccgccatc atgaaccagt agaggagtgc
      781 gggtttctca aaccaggtgt gaccaccgag tgtgggagtg atcaagtcag cccgctggta
      841 catctcgcgc gcaacctggg catagcgcgg ctcatccggt ccgacgaggg gaagataacc
      901 aaggccgtaa agatacgaaa cgacgatgat tgcgaaaaag agtagcagcc agactcgctt
      961 ggcaagggtt ggaagttgca agtatagtcg ctccgcgcag gcagtaggca gtcagcctaa
     1021 gcagaaggca ggaaagataa gcgtgctttc gttctgccta caaaacgcac ctaacctaaa
     1081 gcaaaaagag aaaacagtaa aatcgtgtcc ttgcgaaccg cagtcatcgg cgtcggccac
     1141 ctgggacgac agcatgcgcg ccttcacggg tcactggccg ccgagggact atcagagttt
     1201 gtagcagtct gcgatcaaaa cgctgaaact gcgcgtacga tcgccgcaga acgaagtgtc
     1261 tcgtggaata cagactggcg cgagttgctt ggccaggtag acgcggtcag tctggcggtc
     1321 ccaactgtct cgcattgtga aatcgcttgc ggcttgctgg aaaaaggcat tcacgtttta
     1381 gttgaaaagc cgattgcgcg tacgctagcc gaagccgacc agatgattgc cgccgctgac
     1441 aaaggaaacg ccctgttaca agtcgggcat ctcgaacgct tcaacccggc tttagtcgcg
     1501 ctccggcctc acgtgcgtaa tcctgtctat ttcgagatcc atcgcgtcgg cgagtttacc
     1561 gcgcggtcgt tggatatcga tgtggtgttg gatctgatga ttcacgatct ggatattgtg
     1621 cagtggctgg tgggcgaaga agtggaagta acggaactgc acgcggttgg gattccgatt
     1681 ctgacaaaca aagtggacgc ggcaaacgcg cggctggagt tttcatcagg ggcggtggcg
     1741 aacattaccg cttcccgagt cggcaccgag aagattcgca agatgcgttt ctttcaaccg
     1801 cacgactacg ttgccgttga ctacaccacc aagcgcgcat caatcagcgg cctcgcgccg
     1861 ccgactgcgg agggtttatg gcccggcgtt caggtgcgaa atctcgaagt ggttgacgtg
     1921 gaaccgctgc ggatggagat agccggattc ttcactgcgg ccagggacgg cttgccgtca
     1981 cctgtttctg gccgagacgg tcgcaatgca ctttcactcg cgctgcgcgt attggagagc
     2041 attcacgaac acggtcgccg atcaaacgtc gccgcgctca gtgcgcaaac tccacgtcgc
     2101 taatcagcga gctaatctcc ccgtcgaaaa tcggcaatcg gcaatcggca atcggcaatc
     2161 ggcaatcgcc aatctgaggg ctggcatctg ttagactctc agctcatgtc agacgctgca
     2221 cctacgcgcg aaatccacat ctcagcaacg ctctcagaac gaattgcggc cggcgacttt
     2281 ccgtccgccg tttatctcat tgccgagaag ggtcaaccgg tgtttgccga cgctctcggt
     2341 catgcagtag tggatccata ccgcatcacg gcgtcactca acaccattta tgacctggct
     2401 tcgctgacca agcctttaat cacgggaatg ctctgcgccc agcttgtcga aaaaggcgaa
     2461 ctcaccctcg atagcgcagt agctaactac ctgccggagt tcgatcgcac cgatcggcag
     2521 atgatcacgg tgcgagaact cttaactcac acatcgggac ttcccgcgtg gcgtccgctt
     2581 tacatccttg ccgaaggtga acgtgaacgg gcgctgggcg tgatcgtcaa cgaggctctg
     2641 gagtacaagc cgggaacgcg agtggtctac agcgatcttg gcttcatcgt cttggggttc
     2701 ctgctggaga ggctaatagg aagaggtctc gacgagctgg cgcgaactga aatcatccag
     2761 ccattaaagc tccagcacac tttttttaat ccggagatgg ctttgcagac gggaatcgct
     2821 gcgtgtgaaa caggcaacgc ttacgagc
//
LOCUS       DASUEX010000569         2448 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_188065, whole genome shotgun sequence.
ACCESSION   DASUEX010000569 DASUEX010000000
VERSION     DASUEX010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188065"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(317..1120)
                     /locus_tag="VFV61_09515"
     CDS             complement(317..1120)
                     /locus_tag="VFV61_09515"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX4900759.1"
                     /translation="MSTVLRKGTSHEVNSAHSFEAERLQLKHYDEIAADYDAHYSDAQ
                     SCEYRRRFFYEPMFEGIALAGTEVLDAMCGSGQTTEYLLKQSASVTGLDISNQTMESY
                     QAKWPAAKALRRSLLDSELPEASFDCICVVGGLHHVHPHVPAAVAEIHRLLKPRGYFC
                     FMEPHSGSLPDVIRKFWYKHDHYFSDNEAAIDLNDLERQFSSRFSFHKVKYQGNLAFL
                     LVLNSLIFRIPPRLKPFYSGPLISLEAVISRFQTKLTSCFVVAQWQKKL"
     gene            complement(1117..>2448)
                     /locus_tag="VFV61_09520"
     CDS             complement(1117..>2448)
                     /locus_tag="VFV61_09520"
                     /inference="COORDINATES: protein motif:HMM:NF021635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HEX4900760.1"
                     /translation="ALSMVGALGVYFWARTFLSQTAAAWAGIFYALAPYHVNQLYQAT
                     LFAEYAAAAVLPFAFAFVDRVCIHGRRRDIAGLAFVYAILLFTHLPLAIIGSLALLVY
                     SAVYLAKSRKLITIAKLSLAVALGLCASAVYWVTMIAEMSWIAVNRVHPDASVDYRAN
                     FVLQTISSDNLNVWWMNILSLMTLLLFAPLVVFLRRSAAAQSKTLKCLLPVIVMTLCA
                     CFMALPLSRPLWDVWPQLQQVQFPWRWLAMVSMGGSLIAGAAVSTWKADPRALPRQWR
                     TLVLGAMIISIAFTLSHVVREAQYLSPAKFEETLSEIRGSASVNYWFPIWANSNAPQM
                     HHQIEVDSRQLSIYSWQAEHRQFAVAAGPATEARVRTFYYPHWSATTNGISLPTRPAA
                     DGALLISLPTEAVRVSLDFREPARNRFSAAASLTGLCLIAALAFPLRKQRI"
BASE COUNT          612 a          617 c          691 g          528 t
ORIGIN      
        1 gaagatcgct gcggcggtga gaagcaaaac gaagagaacc aggttataga gcacgcgatc
       61 gcgcacagct tcgcgaaacg tattgcgggc aattgccgcg attcgtttgg ctgttgacca
      121 ttgactacca gatgagcttg ctgtgagttg tccggcggtt gatttggcgc tgttcattct
      181 cagtatgcga gagcgagttt cagcttattt aatctatggt aattcgtaaa gagttgattc
      241 ccggagagta gagcgaggct cggcaaaaag tctcgaccta actgactcgt cgtacgcgga
      301 tgtggtccat ttggcgtcac aattttttct gccattgtgc taccacgaaa caggaggtca
      361 actttgtctg gaatctactg atgacggctt ccaacgatat caatggcccc gagtaaaaag
      421 gcttaagccg gggaggaatg cgaaagatca atgagttcag gaccaagaga aaagcgagat
      481 ttccctggta cttcactttg tgaaaagaga accgggatga aaactgccgc tccaggtcgt
      541 tcagatcgat cgccgcttca ttgtctgaga agtagtggtc gtgtttgtac caaaactttc
      601 gaattacgtc tgggagtgag ccgctgtgcg gttccataaa acagaagtaa ccacgcggtt
      661 tgagcaggcg gtgaatttcc gcgactgctg ctggtacgtg gggatgaacg tgatgcagcc
      721 cgcccacgac gcaaatgcaa tcaaatgatg cttcgggcaa ctcggaatcg agcagggaac
      781 gccggagtgc cttcgcggcc ggccattttg cctggtacga ttccattgtc tgatttgaga
      841 tgtcgagccc ggtcaccgat gctgactgct tcaacaagta ctcggtagtc tgtccactgc
      901 cacacatcgc gtccaagact tcggttccag ccagcgcaat cccttcaaac atcggctcgt
      961 aaaaaaaccg ccggcggtat tcgcagctct gggcatcgct gtaatgcgcg tcgtaatcag
     1021 cggcaatctc gtcgtagtgt ttcagttgca gccgttcggc ttcgaatgaa tgcgctgaat
     1081 tcacttcgtg actggttcct ttccgcaata ccgtgctcat attctttgtt tgcgcaatgg
     1141 gaaggcaagc gcggcaatca agcacaaacc cgtcagactc gctgcagccg agaaacggtt
     1201 tcgcgctggt tctcgaaagt cgagacttac cctcacagct tcagtgggaa gggaaatcaa
     1261 taaggcgccg tctgctgctg gcctggtggg cagcgaaatg ccattagtcg tagcagacca
     1321 gtgcggatag tagaaagtgc gaactcgtgc ctccgtcgca ggtcctgcgg caaccgcgaa
     1381 ctgccggtgt tcagcttgcc aggaatagat agaaagctgc cggctgtcta cttctatttg
     1441 atggtgcatt tgtggcgcgt tggagttggc ccagatagga aaccagtaat tgacactggc
     1501 gctgcctcta atctccgaca gagtttcttc aaatttcgcc ggagaaaggt attgtgcctc
     1561 tcttaccaca tgggaaagag tgaaagcaat tgaaatgatc atcgcgccga gcactagcgt
     1621 gcgccattgt ctcggcaacg ctcgtggatc tgctttccag gttgaaacag cggctcccgc
     1681 gatcagtgaa cctcccattg agaccattgc cagccaacgc cagggaaact gtacctgctg
     1741 caattgaggc caaacgtccc acagtggccg actcaacgga agggccatga agcaggcgca
     1801 cagggtcatg acgatcactg gcagtaggca cttgagtgtc tttgactgcg ctgcggcaga
     1861 tcgacggaga aacactacca gcggagcaaa taagagcaat gtcatcaggg acaggatgtt
     1921 catccaccag acattcaggt tgtctgaaga gatcgtttgc aggacaaagt tggcgcgata
     1981 gtcgaccgag gcatccgggt gcacccggtt aacggcgatc cagctcatct cggcaatcat
     2041 cgttacccag tacacggcag atgcacacaa gccgagggcg actgccagtg acaactttgc
     2101 gatcgtaatg agctttctgg atttggcgag gtagaccgcc gagtaaacca gcaaggcaag
     2161 cgagccgatg atggcgagcg gaagatgagt aaacaatagt atcgcgtaaa cgaatgccaa
     2221 cccggcgatg tcacgtcgcc gtccgtgaat gcaaacccgg tcaacaaaag cgaacgcgaa
     2281 aggcagaacc gcagcagccg catattcagc gaagagcgtt gcctgataaa gctgattcac
     2341 gtggtagggc gcaagtgcat agaagatgcc ggcccaggcg gccgcagttt gagagagaaa
     2401 cgttctggcc cagaagtaaa ccccgagggc gccaaccatt gagagtgc
//
LOCUS       DASUEX010000570         3780 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_911199, whole genome shotgun sequence.
ACCESSION   DASUEX010000570 DASUEX010000000
VERSION     DASUEX010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3780
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_911199"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(173..1522)
                     /locus_tag="VFV61_09525"
     CDS             complement(173..1522)
                     /locus_tag="VFV61_09525"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX4900761.1"
                     /translation="MKGLNTYAVCALVFCGLWASLSNVNSAKLFAQQAPSRPDITAEL
                     VKPHVVTLADDKYEGRGAGYPGERKAADYIAAEFKRIGLMPGGDNVRGRRSYFQEFKF
                     QPWHPVVPWEVLSSRNVLGLIEGTDPILKNEIVVIGAHYDGQGRTGQADPLRLAPSDD
                     NLTKDEIWNSANDNAASIASILEIARHIKNGKINTRRSLLFIAFGAEEHGMAGSLHYV
                     SHPVFPLHNHVAMINLEKVGRSPEKALTVTGGASSSVWQQVFKTAGERTGTKVAPGSP
                     YSFPDSDHYPFAASRIPAIMLLVPTSVDSHQPSDSWDKIDFSRAAEVARYSLATLLEL
                     ANQPQTPDFIASPIPDLGLIAHLITAAESDAAGLGAEESGLKVTGVVAGLPSAAAGLQ
                     PGDLILEFAKRRFRRDDTVAALMAVHRQILEGKLGYRLPVTILRNKKQSELVINLQR"
     gene            2162..>3780
                     /locus_tag="VFV61_09530"
     CDS             2162..>3780
                     /locus_tag="VFV61_09530"
                     /inference="COORDINATES: protein motif:HMM:NF024810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HEX4900762.1"
                     /translation="MSMQFFSPFTSKGLTPHRLLFAEPKLKRNRTRLLQLLGLVALAT
                     IALAATVSAANSVRELILDLGLSGVVGSVSQPSQPKAALNNSLLTPAPQGNAMTVERR
                     GHTATRLADGRVFIVGGENTSGVLNQAEIFDPTTGTFSPAATLSNARTDQSATQLTDG
                     RVLIAGGRGAAGPLTTCEIFDPATGTFTSGSAMSVARAGHSATLFSDGRIFIAGGDQN
                     GSAEIFDPATGAFTLVGANLSSARSMHSATLLQSGRVLIVGGRDAGGNAVNSVEIFDT
                     TTSTFSAVASVLKVARVQPHLRVLFDGKVQIIGGSDDGSMEIYDPLSDTIGAYAHVIP
                     ESDPCAGLPAQVLASQTRAALFHNGQLDPLFDRAGQTITEMNGQALVAGGGTSSGAVL
                     NSFAVVASSSASITTDKLDYAPGETVLISGRGFQPGETVRLKIHEDPHTPQERGFDVV
                     ADADGNFTGTYLVMDYDLNMKFVVGARGLSSGMTAQTTFTDNRVITAATVNGGATTTV
                     AAGATITAAVTVDTTTSGGGGENWFSTSWRFA"
BASE COUNT          827 a         1069 c         1030 g          854 t
ORIGIN      
        1 ccttagtgaa aaaccctata atcgccgacc ttcgcaacct gatgattcgg ttgctgaatg
       61 cggtcgctaa cgctcccgct tccgtattct tcactattcc ctgttgcact tctaagttga
      121 atcccaccgg gtgaaggaac ttctcgactt gaggattttg aaaaaccgag cgctaccgct
      181 gcaagttaat aactagttcc gactgcttct tattgcggag aattgtgacc ggcaggcgat
      241 aacctaactt cccttcaagg atctgacgat gcactgccat gagcgcagcg acggtatcat
      301 cacgccgaaa tcgtcgtttg gcgaattcga ggatcaagtc gcctggttgc aaccctgccg
      361 cagctgacgg aaggcccgcg actactcctg tcactttaag cccgctttct tcagcgccca
      421 agcctgcggc gtcagactcc gcagctgtga tgaggtgtgc gataagtccc aaatcaggaa
      481 tcggagaggc aatgaaatca ggagtctgcg gttggttagc gagttccagg agggttgcca
      541 gcgaatatcg cgcaacttca gccgcgcggg aaaaatcaat cttatcccag ctgtcggatg
      601 gctgatgtga atcaacactc gtcgggacca acaacatgat ggcgggaatg cgagaggcgg
      661 cgaacggata atgatcgcta tccgggaaag agtaagggct gccgggcgcg accttcgtcc
      721 cggttcgctc cccagccgtt ttgaaaacct gctgccacac tgagctgctg gctccaccgg
      781 taacagtcag tgctttttcg ggagagcgcc ccaccttttc gagatttatc atcgccacgt
      841 gattgtgtaa gggaaagact ggatgactca cataatgcaa tgagccggcc attccatgtt
      901 cttccgcgcc aaaagcgatg aaaaggaggg aacgcctggt gttgatcttg ccgtttttga
      961 tgtgacgagc aatttccaga atgcttgcga tgctggccgc attgtcgttt gcactgttcc
     1021 atatttcatc tttagtgaga ttgtcatcgc tgggcgccag ccgcaatggg tctgcctgac
     1081 cggtgcgacc ctgtccgtcg tagtgcgcgc cgatgacgac gatttcattt ttgagtatcg
     1141 ggtcagtccc ttcgataaga cccagcacgt tgcgcgagct caatacctcc caaggcacca
     1201 cgggatgcca tggttgaaat ttgaattcct gaaaatagct gcggcggcca cgaacgttat
     1261 caccgcctgg catcagtccg atccgcttga actcggctgc gatgtaatct gctgccttgc
     1321 gttcccccgg atagccggcc cctcgtccct cgtatttatc gtctgccaac gtaacaacgt
     1381 gaggcttcac taattctgcg gtgatgtcag gtcgggaagg cgcttgctgc gcgaagagct
     1441 tcgctgaatt cacattcgac agactcgccc aaagaccaca gaacaccagc gcgcaaacgg
     1501 cgtacgtgtt tagtcctttc ataagaatcc cccttgccgt aaaaatacga ggacaaggcc
     1561 acatggttcc gttgactttg ttgcccggcc agcaacgatt gggaaccgga gtttctgcca
     1621 caactgctcg catacgagca actttggatt acgccgtccg caccttccgc cacacttcgt
     1681 gctggctcca aaaaatctct aaaccactcc ccagtcggga ctttgatcca ctcctaaccc
     1741 cactgcccca actgagcatg cctcatcacc tgtgcagtgg cgcagttatt gccaagactt
     1801 gaccacgaat gcgcagagcg tgaaccgttc cttgctcagg agggatgtcg caacgctctt
     1861 cgtatggttt ggaatagtga caaccatcga gcccagggac gagccccgca ggattcagaa
     1921 cctgcggggc tactgatgat cttttagttt ccttccgcgt gcgggaatcg cgctcacaaa
     1981 gtcggtactg cgattctgcg tcaaggcagt tctaaatagt caccccctca ggttcgggca
     2041 tccatgcatc gattgcgcgg atgtcgtatc tcgaggtgtc ggtgtcgctg gcacactcag
     2101 gtaaaccctg cggcagcgct tcttgaaccc gcgcaccgca ctatctttta ggagctccta
     2161 aatgagcatg cagttcttct cacccttcac ctccaaaggt cttactcccc atcgattgct
     2221 gttcgctgag cccaagctaa aaagaaatag aacacgacta ctgcagctac ttggcttggt
     2281 tgctttggca accattgcgt tggcagcgac cgtgtccgct gcgaactccg tgagagagct
     2341 gatacttgat ctgggtttga gcggtgttgt aggcagtgtg tcacaaccat cgcagccaaa
     2401 agctgcgctg aacaactcgt tactcacgcc cgcgccccag ggaaacgcca tgacagtgga
     2461 gcgacgcgga cacaccgcga cgcggctggc cgacggtcgc gtgttcatcg tgggcggcga
     2521 aaacaccagt ggcgtgctca accaagctga gatttttgat ccaaccaccg gaacattttc
     2581 acctgccgca accctcagca atgcgcgcac tgatcagagc gcaacgcagc tcaccgatgg
     2641 acgggtgctg attgccggcg gtcgtggcgc cgcggggcca ttaactacat gtgagatttt
     2701 cgatccggca accggaacct tcacatcagg ttcggcaatg agcgtcgcgc gtgcaggtca
     2761 cagcgcgacg cttttttcgg atgggcgaat cttcatcgca ggcggtgatc agaatggcag
     2821 cgcggagatt tttgatcccg cgaccggcgc gtttactctt gtgggcgcga atttgagcag
     2881 cgccaggtcg atgcattcag ccacgctgct tcaaagtggc cgggtgttga ttgttggcgg
     2941 acgcgatgct ggcggtaatg cggtcaactc agtcgagatc tttgacacga ctacctccac
     3001 tttctccgcg gttgcgagcg tactcaaggt tgcacgtgtg cagccgcacc tccgcgtctt
     3061 gtttgatggc aaggttcaga tcattggtgg tagcgacgat ggctcaatgg agatctacga
     3121 tccccttagc gacacgatcg gcgcctacgc gcatgtgatt cccgaaagtg acccatgtgc
     3181 cggcctgcct gcacaagtgc tcgcctcgca aacgcgcgcc gcgctctttc ataacggcca
     3241 attagatcca ctgttcgacc gcgcaggaca aacgatcact gaaatgaacg gtcaggcact
     3301 ggtcgccggg ggtggcacca gcagcggagc tgtgcttaat tcgtttgccg ttgttgcgag
     3361 ctcatcggct tcaatcacaa ctgacaagtt ggattacgcg ccaggtgaaa cagttcttat
     3421 ctccggccgg ggtttccagc ctggagaaac tgtgagactc aagatccacg aagatccgca
     3481 cacgcctcag gaaaggggct tcgacgtggt ggcggacgct gatggaaact tcaccggaac
     3541 ctatctcgtg atggactacg acctgaatat gaagttcgtt gtcggggcga ggggcttgag
     3601 ttcaggtatg actgcgcaga cgacttttac ggacaataga gtaatcacgg ccgccacggt
     3661 aaatggtggt gcgacgacca ctgttgctgc tggagcgacc atcacagccg cggtcacagt
     3721 ggataccaca acgtcagggg gcggcggaga aaactggttc tcaacctcct ggagatttgc
//
LOCUS       DASUEX010000571         4791 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1479462, whole genome shotgun sequence.
ACCESSION   DASUEX010000571 DASUEX010000000
VERSION     DASUEX010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4791
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1479462"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(181..2037)
                     /gene="nosZ"
                     /locus_tag="VFV61_09535"
     CDS             complement(181..2037)
                     /gene="nosZ"
                     /locus_tag="VFV61_09535"
                     /EC_number="1.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR04246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050304 - nitrous-oxide reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019333 - denitrification pathway [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-dependent nitrous-oxide reductase"
                     /protein_id="HEX4900763.1"
                     /translation="MKGNHSKWTWFGFGTLCVVLLFFTYVGCSKKPKVERGDVSEAAI
                     ASYVAPGDTDEYYLFYSGGHSGNVYVAGVPSMRHISTIPVFAPYPATGYGFDKESKEM
                     LGSFTWGDVHHPALSETKGDYDGRWLFVNDNANNRMARIDLRDFKTKQILGPVPNISG
                     YHGGSFVTPNTEYVLAASRFSVPLPKGTYEPMEDYATKFKGIVAGLAIDPTTGNMSNG
                     WQIVMPPFDFDLGDAGKGASEGWAFWTSYNTERATGKLEVTSTQKDRDYVVAVNWKEA
                     EKAANEGRGVKLIGGVKVIDPKDAPGIVYLIPCSKSPHGVDVSPDGNWIIASGKLQSI
                     TTAFSMAKLLTAIQNKDFTGDEDGIPVLNYEKVREAEVNVGLGPLHTQFDDQGFAYTS
                     LFVESAVAKWKLGTWEMVDKIPISYNIGHLATAEGDTVNPDGKYLIALNKLSHGRHMN
                     VGPSQPESSQLINISGEKMKLIYDAFTEPEPHYAQLIKADKLKPIEVYPREENKNPNA
                     IWDMKDASVSSSGSEVTVKLLAVRSSFEPNKIEVNQGDKVTIYITNIEQTTDELHGFG
                     LLEYNINVVVDPGETKTIEFVADKAGVFPYYCTNFCSALHQEMQGYLLVKPR"
     gene            complement(2131..3177)
                     /locus_tag="VFV61_09540"
     CDS             complement(2131..3177)
                     /locus_tag="VFV61_09540"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX4900764.1"
                     /translation="MKDLDRVDRCVTCHQGMEWKGLDNAPQPFKSHPQEILKKHPLDQ
                     YGCTSCHGGQGFATDMESAHGFVEHWEEPLLGKQVSDLYLVKDRRALMQMNCNTCHRF
                     DRETKGADFINQAKQLVAEKNCRACHTINGRGGTIGPDLTYVGNKSPEQYDYSRMLGV
                     KTAFGWHVAHIQNPKSLVPETVMPNFGFNSREAQGLALLMMSWRRTAIPANYMSGVKF
                     TEQQTAEEIEKEKRMLEGEGAFFVKKGCFVCHSVSTLGVESASKIGPDLSEAVTDVQS
                     RFGKQLEDFLNNPTGTMAVVLSTQIILSPEEKREALEKLKIAYQRKIEQQAQSAAAQK
                     NQPQAKPTPTPTKK"
     gene            complement(3381..4727)
                     /locus_tag="VFV61_09545"
     CDS             complement(3381..4727)
                     /locus_tag="VFV61_09545"
                     /inference="COORDINATES: protein motif:HMM:NF012263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HEX4900765.1"
                     /translation="MVTKRSALSLLKNRSATWEKIKWSWLPKSNREAGDSVVKNFLLH
                     WFPAKVTRQSLSWNYSFWLGTISAVLFLILVLTGIPLMFLYVPSVERAYLSVKDIEFA
                     VSYGWFLRGLHRISAHLMVAVVFLHMVRVFLTGAYKNGTAANQNRPLNWIIGIVLLLL
                     TLLLSFTGYLLPWDQLAYWAITVGTNIASAAPLVGESMRFFLLGGTTIEQNTLIRFYV
                     LHCFFLPLLVTLLFSYHMWRVRKDGGLACADRLSLEQKKEEVAPAKSKTYSLLGITSG
                     SSVHVETTIVDDDQHTVMSSPNLTRRLLVVSLGTLVVASILTIIFRAPLEAAANPAVT
                     PNPAKAPWYFLWLQELVTTTTFTIGGFTVNGALIGGIVLPGLLVLAALAWPYLDKSSI
                     RSVGVWWAADRRRQNLVFLVIVAAILILTVIGTFLRGPYWNWYWPWQSWPEMPRKF"
BASE COUNT         1001 a         1353 c         1299 g         1138 t
ORIGIN      
        1 gtggcaactc aaagaaagtt gtttccttat caagaaaagt gctcatgttt tggaccactc
       61 cttttcgctg ccgcttttga atttgctcgc ctaacctggg ttacgctgcg cgtcacccag
      121 ccgttacaaa ttgacaagcc tcgggggctg gccacatcga cgaacttcgg tctcgtacaa
      181 ctaccttggc ttcactaaca aatacccttg catctcctgg tggagtgcgg agcagaagtt
      241 ggtgcagtag tagggaaaga cgccggcctt atccgctaca aactcgattg tttttgtctc
      301 ccccggatca accaccacat taatgttgta ttcgaggaga ccgaatccgt gtagttcatc
      361 cgtcgtctgc tcgatgttag tgatatagat ggtcaccttg tcgccctgat tgacctcgat
      421 cttgttcggc tcaaagctgc tgcgcactgc gagcagtttt acggtcacct cggaaccgct
      481 actgctgact gacgcgtcct tcatatccca gatggcgttc ggattcttat tctcctcacg
      541 gggatagacc tcaatcggct tgagcttgtc ggctttaatg agttgtgcgt agtgcggctc
      601 tggctcggtg aacgcgtcgt agatcagctt catcttctcg ccactaatat ttattagttg
      661 cgagctttcc ggctgcgacg ggccaacgtt catgtgccgc ccgtgggaaa gcttgttaag
      721 ggcgatgaga tatttcccat caggattaac cgtgtcgcct tcggcggtgg cgaggtggcc
      781 gatgttgtaa ctgatcggga tcttgtccac catctcccag gtgcccagct tccatttggc
      841 gacggcgctc tcaacaaaga gagaggtgta ggcgaagcct tgatcgtcaa actgcgtgtg
      901 cagcggtcct aaaccgacat tcacttcagc ctctctgacc ttttcatagt tcagcaccgg
      961 gatgccgtcc tcatcgcccg tgaagtcctt gttctggata gctgtcagca gcttcgccat
     1021 gctgaaggcc gtggtgattg actggagttt tccgctggcg atgatccagt tgccgtcggg
     1081 actcacgtcc acgccgtgcg gactcttgct gcacggaatg agatagacga tgccgggcgc
     1141 gtctttcggg tcaattactt tgacgccacc gatcagcttc acaccccggc cctcattggc
     1201 agctttctca gcctctttcc aatttacggc aacaacgtaa tcgcggtctt tctgagtcga
     1261 agtgacctcg agtttgccag tggcgcgctc ggtgttgtag ctagtccaga aagcccagcc
     1321 ttcgctggcg cccttgcctg cgtcaccgag atcgaaatca aacggcggca tgacgatctg
     1381 ccaaccattg ctcatgttgc ccgtggtggg gtcgatggcc agacccgcga cgatgccctt
     1441 gaacttagtc gcatagtctt ccattggctc ataagttccc tttggcaagg gcactgaaaa
     1501 gcgggacgca gccagcacgt attcagtatt cggggtcacg aacgaaccac cgtgatatcc
     1561 cgagatgttg gggacggggc cgagaatctg cttggtctta aaatccctga gatcaatccg
     1621 cgccatacgg ttgttcgcgt tatcgttgac gaacagccaa cgcccgtcgt agtcaccctt
     1681 ggtttcgcta agggcgggat gatgcacgtc gccccaggtg aagctgccca acatctcttt
     1741 tgactctttg tcgaatccgt agccggtcgc cgggtacggc gcaaagacgg gtatcgtcga
     1801 gatgtgacgc attgacggca ccccggcgac gtagacgttg ccggaatggc cacccgaata
     1861 aaagaggtag tactcgtccg tatcgcccgg agccacatag ctggcgatgg cggcctcaga
     1921 cacgtcgccg cgttcgacct tcggcttttt cgagcagccc acataggtga aaaacaggag
     1981 cacgacacac agcgtgccaa aaccaaacca tgtccatttc gaatgattgc ctttcatctt
     2041 tgaatctcct tctgaggaat ttcgttcacc ctctgctctg tgggccgctc gctcgtcctg
     2101 ttgcgcgctc tgattcccat cgccgacggc ttacttttta gtcggcgtgg gcgtcggctt
     2161 ggcttgcggt tggttcttct gcgcagccgc ggactgggcc tgctgttcaa tcttccgttg
     2221 ataagcgatc ttcagtttct ccaacgcctc tcgcttctct tccggcgaca aaatgatctg
     2281 ggttgacaag acgacagcca tcgtgcccgt cgggttgttc aaaaagtctt caagctgctt
     2341 gccgaaacgg ctttgcacgt ccgtcaccgc ttcggaaaga tcggggccga tcttggaagc
     2401 cgattcgacc cctagcgtgc tcaccgagtg acagacgaaa catcccttct tgacgaagaa
     2461 tgcgccctcg ccttcgagca tgcgtttctc tttctcaatc tcttccgcag tctgttgctc
     2521 ggtgaatttc acaccggaca tgtagttggc tggaattgct gttcttctcc aactcatcat
     2581 gagcaaggca agcccctgcg cctcgcggct gttgaaaccg aagtttggca tgaccgtttc
     2641 cggcaccagg gatttcggat tttgaatgtg cgcgacgtgc cagccgaagg ccgtcttgac
     2701 gccgagcatc cgtgagtagt cgtattgctc cggcgattta tttcctacgt aggtcaggtc
     2761 cgggccgatg gtccctccgc gaccgttaat cgtgtggcaa gcacggcaat ttttctcggc
     2821 caccaactgc ttggcctgat tgatgaagtc ggcccctttg gtttcgcggt cgaagcgatg
     2881 acaggtattg cagttcatct gcatcagcgc gcgtcggtcc ttcaccaggt agagatcgct
     2941 cacctgttta ccgaggagag gttcttccca gtgctcgaca aaaccgtggg cggattccat
     3001 atcggtcgca aaaccctggc cgccgtgaca gctcgtacag ccgtactggt cgagcgggtg
     3061 ttttttcaga atctcctgag ggtgcgactt gaatggctgc ggagcgttgt ccaatccttt
     3121 ccactccatt ccctgatgac aagtcacaca gcggtcaacg cgatccaggt ctttcaccca
     3181 aatctgctgt agtccgcgag gaactgcggc cgctctttgc gctccatact tctgctctac
     3241 catctcctga aactcgtcct ggtagccctt ccattcagga gtgaagtagt tccagaagat
     3301 gaagccggtg ataatgaaag ccagcccgcc aaacgagtag agcaagacgg aatctttctt
     3361 aaacaccgcg aagtcctcct ttagaatttg cgtggcatct caggccagct ctgccagggc
     3421 cagtaccagt tccaatacgg cccacgcagg aaagtgccga taaccgtcaa gatcaaaatc
     3481 gccgccacaa tcactagaaa tacgaggttc tgtctgcggc gatctgccgc ccaccagacg
     3541 ccaactgatc gaatcgacga tttgtcgagg tacggccagg ctaaggctgc gagtacaagc
     3601 aagcccggca ggacgatccc gcctatgagc gctccgttaa cggtgaagcc gccgatggta
     3661 aaagtcgtgg tggtgacaag ttcctgtagc cagaggaagt accagggcgc tttggctggg
     3721 ttgggcgtca ccgccgggtt tgctgccgct tccagtgggg cgcggaaaat gatggtcaag
     3781 atggaggcga ccaccagtgt ccccagtgat accaccagca gtctccgcgt gaggttcggt
     3841 gaagacatga ccgtgtgctg gtcgtcgtca actatcgtag tctcgacatg cacggacgag
     3901 ccgctggtta ttcccagaag cgaataagtc ttactcttgg ctggggccac ttcctccttt
     3961 ttctgctcga gcgagagccg gtcagcacac gccagcccgc catccttcct tacacgccac
     4021 atgtgatatg agaacaacag cgtgaccagg agtggcagga agaagcagtg caggacgtag
     4081 aagcgaatta gcgtgttctg ttcgatggtt gtgccgccga gaaggaagaa ccgcatgctt
     4141 tcgccaacga gcggcgccgc cgatgcgatg ttcgtcccga cggtaatggc ccaataagcc
     4201 agttgatccc agggcagcag gtatcccgta aacgagagca gcagtgtgag gagcagcagc
     4261 acgatgccaa taatccagtt cagcggccgg ttttgattgg cggcggtgcc attcttgtac
     4321 gcgcctgtca ggaacactct aaccatgtgc aggaaaacca ccgctaccat gaggtgagca
     4381 gagatgcggt gcaggccccg caggaaccag ccgtaagaga ccgcgaattc aatatccttt
     4441 acggagagat aggcgcgctc gaccgaagga acatagagga acatgagtgg tatgccggtg
     4501 agcaccagga tgaggaagag cacagccgag attgtgccaa gccagaagga gtagttccac
     4561 gaaagactct gcctcgtaac ttttgctgga aaccagtgca gcagaaagtt ctttacgacg
     4621 gagtcacccg cctcgcgatt actcttgggc agccagctcc atttgatttt ttcccaggtt
     4681 gcgctcctgt tcttcagcaa gctcagggcc gaacgttttg tcaccatgag ttgattcctt
     4741 cgctaaggcg tcgatccaaa atcttgacgc gcgcgtggtc cttcaagtct t
//
LOCUS       DASUEX010000572         3801 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1238349, whole genome shotgun sequence.
ACCESSION   DASUEX010000572 DASUEX010000000
VERSION     DASUEX010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3801
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1238349"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3071
                     /locus_tag="VFV61_09550"
     CDS             <1..3071
                     /locus_tag="VFV61_09550"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX4900766.1"
                     /translation="ARTSNTGASRFTLVLTGPLSGEMRVLRAPIALFLDFIKEKGFAS
                     DVRVITGAGFVAFDGASDQNEQQLLGCVQQFISERMQAAEFHPDSWPPIIIRDPQEAQ
                     PKLSAVAGDKYSYRQMDDFTDLLEKTLKAVPVVSKVSRSGVLSEKVYLLYSQERLAAY
                     GMQPKSLENALSSRNISTPGGQVNVEGKNVSLDPSGEFKNEREIGDVLIPTNAGTSVY
                     ARDLVDIVRGYDNPARYLNYFNWRDGQGNWRRSRAVTLSVQMRSGEQIDNFGKQVDTA
                     LAGLKQQLPEDLIFARTSDQPLQVEENIDLFMKSLYEAIFLVVIIALIGFWEWRSALL
                     MALSIPITLAMTFGMMYALGIDLQQVSIATLIIALGLLVDDPVVAGDAIKRDLALGHK
                     PVIAAWLGPTKLATAILYATITNIVAYLPFLLIKGATREFIYSLPIVLTCSLIASRLV
                     SMTFIPLLSYYLLKPKAEKPIAERRQQGFAAKYYRVGTWAINHRWKVMAGGLVFLLIG
                     GGLMTQLKTQFFPKDLSYLSYVDVWLPEDAPLSATNEAATKAEEIIREVVGEYSHEHA
                     KEGEVLQSLTTFVGGGGPRFWFSVSPELQQLNYAQIIIQVKDKHDTSHLVGLLQSALS
                     ARVPGARIDVRQLESGKAVGLPVSIRLSGEDQQTLRVYADRAKAILAETSFAERVRDD
                     WGDESFAVRLKTDPDRANIAGLTNQDVAMASLTATAGHQVTSLREGDKQIPVVARLKM
                     EERAQLGDLRNLYVYSASGKQKIHLSQVASTEYGMQTEKMRARNQFRTITVAAMPAEG
                     RLASEVMKAARPKLLALEKELPTGYRMEIGGEEEDQVKGFKDLTIVLGISVSMIFLAL
                     VFQFKNAIKPFIVFAAVPFGMVGALAALWIMGAPFGFMAFLGIVSLVGVIVSHIIVLF
                     DFIEEKHAEGEPLIEALLDAGIMRLRPVLITVGATVIALFPLASHGGPLWQPMCYAQI
                     GGLSVATFITLLLVPVIYSIFVLDLKLVKWDEAVEANETATDGQRVGELS"
     gene            3068..>3801
                     /locus_tag="VFV61_09555"
     CDS             3068..>3801
                     /locus_tag="VFV61_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900767.1"
                     /translation="MKFRRSRGRLVGAFESEFALEVLASDQLRVNILIGVIVSAVLVV
                     SVLLVSSFDQFRRAFHGNIKGFLISFSVVAGSALVCLLFERATIGRMIRKQKRVSKWV
                     QYLSALVETSIPTAGMIVGSMFLGPIYSLFTPAAFVYPIFIVLAALRLNFKLCVFTGA
                     VAGIEYTALALYFISQGSNDAIEPILSGVPHHLVKGFLLFFMGVVTGLVTQQTRKRIL
                     KSFETVEERNRISRTFGEYVSPVVMD"
BASE COUNT          842 a          981 c         1068 g          910 t
ORIGIN      
        1 gcgccaggac ctcgaacact ggagcctcga gattcacctt agtactcacc ggaccattgt
       61 ctggagagat gcgtgtgttg cgcgcgccta tcgcgctgtt tcttgacttc atcaaagaga
      121 aaggtttcgc cagcgacgtc cgtgtgatca caggcgcagg gtttgttgca tttgacggtg
      181 cttcagatca gaacgaacag cagttgctcg gctgcgtgca gcagttcatc agtgaaagaa
      241 tgcaggccgc tgaattccat cctgactcct ggccgccaat tattattcgc gatccccaag
      301 aggcacagcc gaagttgagc gcggtagccg gcgataaata tagctatcgc cagatggatg
      361 atttcactga cctgctcgaa aaaactctga aggcagttcc cgtcgtttca aaggtcagtc
      421 gctcgggcgt cctgagcgaa aaggtttatc tgctgtattc gcaggagcgt ttggctgctt
      481 acggcatgca gccgaaaagt ctcgagaacg cgctgagtag tcgcaacatc tcgacgccgg
      541 gaggccaggt aaacgtcgaa ggcaagaacg tcagcctcga tccttccggt gagtttaaga
      601 acgaacgcga gatcggcgat gtgttgattc ctacgaacgc cggaacctcg gtttatgctc
      661 gcgatctcgt ggacatcgtg cgtgggtacg acaatccggc gcgctatcta aactacttta
      721 actggcgcga cgggcaggga aactggcgcc gcagtcgcgc agtcactctc tcggtgcaga
      781 tgcgctccgg cgagcagatc gacaattttg gcaagcaggt ggatacggca cttgccggat
      841 taaagcagca actgccagag gatctgattt ttgccagaac atccgaccag ccgctgcaag
      901 tggaagagaa catagacctc ttcatgaaga gcctttacga agcgatcttc ctggtggtga
      961 tcatagcgtt gatcggtttc tgggaatggc gctcggcctt gttgatggcg ctatcaatcc
     1021 caatcacgct ggcgatgacc ttcggcatga tgtacgcgct gggaattgat ctacaacagg
     1081 tttcgatcgc aacgctgatt atcgcgttgg gattgttggt tgacgatccg gtcgtggccg
     1141 gcgatgcgat caagcgcgat ctggccctcg gccacaaacc cgtgatcgcg gcatggttgg
     1201 ggccgacgaa actcgcaact gcgatccttt acgcgacgat caccaatata gtcgcatatc
     1261 tcccgttcct tttgatcaaa ggagcgacac gggaatttat ctacagcctg ccaatcgtat
     1321 tgacgtgctc tttgattgcg tcgcggctgg tctcaatgac cttcattccc ctgttaagtt
     1381 attacctgct aaaaccgaaa gccgagaagc cgatcgcaga acgtcgtcag cagggtttcg
     1441 ccgcaaagta ttaccgcgtc ggcacctggg ctatcaatca ccgctggaaa gttatggcgg
     1501 gtggactggt gttcctgcta atcggcggtg gcctcatgac tcagcttaaa acgcagttct
     1561 tccccaagga cctttcctat ctctcttatg ttgatgtttg gctcccggag gacgcaccac
     1621 tatctgccac caacgaagcc gcaactaaag cggaagaaat cattcgtgaa gttgtcggcg
     1681 aatacagtca tgaacatgca aaagagggcg aggtcttaca atcgctgaca acttttgtgg
     1741 gcggtggcgg ccctagattc tggttctccg tgtcaccaga gttgcagcag ttgaactatg
     1801 cccaaatcat cattcaggtc aaagacaagc acgacacctc tcacttggtt ggtctgctgc
     1861 agagcgcatt gtcagcgcgg gtgcccggcg cccgcatcga tgtgcgacaa cttgaatcgg
     1921 gaaaagctgt gggactgccg gtctccattc gcctgtcggg tgaggaccag cagacgcttc
     1981 gcgtgtatgc ggaccgggct aaggcaatcc tggcggaaac ttcatttgcg gagcgcgtgc
     2041 gagacgactg gggtgacgaa agctttgccg tccgtctcaa gaccgatccc gatcgcgcga
     2101 atattgccgg attgacgaat caggatgtcg cgatggcctc gttgactgca accgccggac
     2161 accaggtaac gagtttgcgc gaaggcgata agcaaattcc ggtagtcgct cgcctcaaaa
     2221 tggaagagcg cgcgcaactc ggtgatctga gaaacctcta cgtctattcg gcatccggga
     2281 agcagaagat tcatttgagt caagtggctt ccaccgagta tggaatgcag actgagaaga
     2341 tgcgagcacg caatcagttt cgaacgatca cagttgcggc aatgccggcg gaaggccggc
     2401 tggcttcgga agtaatgaaa gcagcgcgtc caaaattgct ggcgctcgaa aaggaattgc
     2461 ctactggtta tcgcatggag atcggcggcg aggaagaaga ccaggttaag ggtttcaagg
     2521 acttgacgat cgtacttggt atctcagtct cgatgatctt cctggccctg gtatttcagt
     2581 tcaagaacgc gatcaagccg ttcatcgtgt ttgcggcggt gccgtttggc atggtgggcg
     2641 cgctcgctgc gctctggatc atgggagcac cattcggctt tatggcgttc ctcggaatcg
     2701 tcagcctggt aggcgtgatt gtcagtcaca ttatcgtgct gttcgacttc atcgaagaga
     2761 agcacgccga aggcgagcct ctgattgaag cattgctcga cgctggcatc atgcgtctgc
     2821 gtccggtact aatcactgtg ggcgccaccg tcatcgccct gtttccgttg gcatcacacg
     2881 gcggcccgct ctggcaaccg atgtgttacg cccagattgg tggtttaagc gttgcgacat
     2941 ttatcacact gctgctggtg ccggtgatct attcgatctt cgtgctcgac ctgaagctag
     3001 tgaaatggga tgaggccgtt gaagcgaatg aaaccgccac ggacgggcaa cgtgttggag
     3061 aattgtcatg aaatttcgac gatcaagagg tcgcctcgtc ggcgcattcg aaagcgaatt
     3121 tgctctggag gtcttagcca gcgatcagtt gcgggtcaac attttgattg gtgtgatcgt
     3181 cagtgcagtg ctggttgtct cggttctatt agtctcttcc ttcgatcaat ttcggcgcgc
     3241 attccatggc aacatcaagg gtttcttgat cagctttagc gtcgtcgccg gctcagccct
     3301 tgtttgtctg ttgtttgaac gagcaacaat cggtcgcatg attcgcaagc aaaagcgcgt
     3361 ctcgaaatgg gtccaatacc ttagcgccct cgttgagaca agcatcccaa cggccggaat
     3421 gatcgtgggc tcgatgtttc tggggccaat ttacagcctc ttcactccag ccgcctttgt
     3481 ctatcctatt tttatagtgc tcgctgcgct gcgactgaat ttcaaattat gtgtgtttac
     3541 cggcgcagta gccggcattg aatatacggc gctggcgctc tacttcatta gtcagggatc
     3601 gaacgatgct atcgaaccca tcctttccgg agttccccat cacctggtaa agggcttcct
     3661 gcttttcttt atgggtgtgg tgactgggct ggtcacacaa caaaccagga aacggatcct
     3721 gaaatctttc gagacggttg aagagagaaa tcgaatcagc cgaacgttcg gcgagtatgt
     3781 ttcaccggtc gtcatggaca a
//
LOCUS       DASUEX010000573         4062 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_912226, whole genome shotgun sequence.
ACCESSION   DASUEX010000573 DASUEX010000000
VERSION     DASUEX010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4062
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_912226"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..957)
                     /locus_tag="VFV61_09560"
     CDS             complement(<1..957)
                     /locus_tag="VFV61_09560"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX4900768.1"
                     /translation="MPRGITRILSLTLSVALYASLVVFNVEVFAQRKGRSESSTAAKP
                     AVDKPVPDRSQQVEPVKPITKVAVGVPLVVTDAAGQYVSDLKQSDLEVSEDGVKQTVA
                     LFQAPSAPSWVVLLLDTSVSTQGKLGDVRRAANVFVDQLQNTDRVKVISFDDQVRELS
                     EFSADRAVVKKAILSAQTGYSTKFYDGMNVALEALREVDGRKAIVIFSDGVDYRSDYA
                     TAESTVRFLEQDGIVVYPIRFSTRVAAEKLAREQAGSQLPTREVVRSTSGSPDPVPGE
                     VPSSEPKTGPLGLPSPEEILRRRQDSRRNRDRLPPGDRPPAGE"
     gene            1079..1879
                     /locus_tag="VFV61_09565"
     CDS             1079..1879
                     /locus_tag="VFV61_09565"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEX4900769.1"
                     /translation="MTTPANQSSPLAGRTVVIVEPEKLSEPVLKELDGCGARVLTCPP
                     IEIAALGSYEQLDEALDHLYGYDWLLFTSVSAVEAFLRRLQHKGFESSALDELRVCAL
                     GDETERLLREEQVHVDVASAIPDARALFDALESFVGGRPALTGLNVLSPRAAVVGDSL
                     TRALTDAGARVDLVPAYRVKPAPGMDPGRMAAMFSAAADYIVLGGSASVSHLSRLFDT
                     LDLGAVLADVSIGCFDEATAKAAQDHGLDSRILPTRSAVKAIAKEVVG"
     gene            1950..3332
                     /locus_tag="VFV61_09570"
     CDS             1950..3332
                     /locus_tag="VFV61_09570"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX4900770.1"
                     /translation="MSLRRVLIIDSDAASAEALHRLIASWNYDVVSESAEARALATVS
                     ETEPAVIVASSTVHSADDFGLLREMRAQQPDTPIIWVTGAGSVEMALHVVQDEGAYHY
                     FERPVDADKLRIVLDRAVELSEAKRENALLRRQLQERGAFGELVGQSEQMRQIYSLIE
                     QVAPSSASVLITGDSGSGKELVARTLHQKSPRKDKPFVAINCSAIPETLMESELFGHE
                     KGAFTGAASRRQGCFELANTGTLLLDEIAEMPPLLQAKLLRVIEERAIRRLGSRSEIS
                     IDVRLLAATNRDPADAVRSGSLREDLLYRLNVFRVHLPPLKDRKDDLPLLAQHLVTQL
                     AEKHERPARFLSPSAISALQFHAWPGNVRELRNVIERAVIICSGEQIERHHFAPYPID
                     QRERLRDEDTISFPVGTPIEEVERQMIMRTLQKTKNNKTRAAVLLGFSLKTLHNKLNL
                     YRERGLLHDE"
     gene            complement(3370..>4062)
                     /gene="dapB"
                     /locus_tag="VFV61_09575"
     CDS             complement(3370..>4062)
                     /gene="dapB"
                     /locus_tag="VFV61_09575"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEX4900771.1"
                     /translation="RTHRGENLKDILGASNIDLIVSGSVGEALETPTDVLVDYTKADV
                     VKAHVMTAIRKGVHVVIGSSGLTDEDFVEINESALAARVGVMAAGNFAITAVLLQRFA
                     CEAAKYLSHWEIIDYASDAKKDAPSGTTRELALRLSEVRKPEVTHPPGDVIGEKESRG
                     TTLNGIQIHSVRLPSYVISVEAIFGASDERLSIRHDAGSGAEPYIQGTLLAIREVRNH
                     VGLIRGLDKILK"
BASE COUNT          877 a         1149 c         1116 g          920 t
ORIGIN      
        1 ttcgcctgcc ggcggacgat ccccaggcgg cagccgatct cggttgcgac gtgaatcttg
       61 tcgtcttctc agaatctcct ccggcgatgg taaaccgagc ggtccagtct tcggttctga
      121 tgaggggacc tctccaggaa ccggatccgg cgagccggaa gtcgacctca ctacttccct
      181 ggtcggcaat tgcgaacccg cttgttcacg cgccagcttc tcagcggcca ctcgcgtgct
      241 gaagcgaatc ggataaacaa cgatgccatc ctgttccaga aaacgcaccg tgctctctgc
      301 agtggcatag tcgctccgat aatccacgcc atcgctgaag atcactatcg ctttgcgacc
      361 gtcgacctcg cgcagagcct cgagcgccac gttcatacca tcgtaaaact tcgtgctata
      421 cccggtctgc gcgctgagaa tggccttctt cacaacggcg cggtcagcgc taaactcgct
      481 cagctcccta acctgatcgt cgaaactaat gactttgact ctatccgtat tctgtaactg
      541 atcgacaaac acgttcgccg cccttctaac gtcgccgagt ttgccctgag tgctgacact
      601 cgtatccagt aacaagacta cccatgatgg cgcactcggt gcttgaaaaa gagcgacggt
      661 ctgcttcacg ccgtcttcgg aaacttcgag atcactctgc ttcaggtctg agacgtactg
      721 tccggcggcg tcagtaacga ccagcggaac gccgacagcc actttggtga tcggtttgac
      781 aggttctact tgctgagatc tgtcgggtac cggcttgtcc accgcaggtt tggcagcagt
      841 ggacgactca gatcgccctt ttctttgcgc gaacacttca acattgaaga caaccaggct
      901 cgcgtacaat gccacgctaa gcgtcagtga taggatccgg gttatccctc tcggcatcga
      961 gtgtctccag cggtacggca tgtttgcgat ttggaatatt ctgtagcgaa cttgggatga
     1021 ccgctacgat gataaggttt gcccggtcga tcacaacgag agcctaacta agagcacgat
     1081 gactacccct gccaatcaaa gtagtcccct cgccggtcgc accgtcgtga ttgtggagcc
     1141 agagaagcta agtgagccag ttctcaaaga gctcgatggt tgcggcgctc gtgtgcttac
     1201 ctgcccgcca atagagattg ccgcgcttgg gagttatgag cagctcgacg aggcgcttga
     1261 ccacctctac ggctacgact ggttactttt taccagcgtg tctgcagtcg aggctttttt
     1321 gcgtcgtctg caacacaagg gttttgagtc cagcgccctc gacgagctgc gggtttgtgc
     1381 gctcggtgac gaaaccgaga gactgcttcg tgaggaacaa gttcatgtag acgttgcttc
     1441 ggcaatacca gatgccagag cactctttga cgcgcttgag agtttcgtag ggggccggcc
     1501 ggcattaact ggactcaatg ttctttcacc gcgcgctgcc gtcgttggcg actcgttaac
     1561 gcgggcgtta accgacgccg gggcgcgtgt cgatctggtt cctgcatatc gcgtcaagcc
     1621 ggcgccagga atggatccgg gccgcatggc ggcgatgttc tccgccgcag cggactacat
     1681 tgtgctgggc ggctcggctt cggtaagcca tctgtcgcga ctattcgata cccttgattt
     1741 aggggcagtg ctggcagacg tatcgatcgg atgctttgat gaagcaactg ccaaagcggc
     1801 ccaggaccat ggtctggact cgagaatcct gcccacgaga agtgctgtta aggctattgc
     1861 gaaagaagtc gttggctaaa cgaccttcac gcgctcgcaa aaattgtcac accaaaagcc
     1921 agtaccactt cgtgttagac tgaactcaaa tgtctcttcg tcgagtgtta ataattgact
     1981 ccgacgctgc cagtgccgag gcgctgcacc ggctgatcgc atcatggaat tacgacgtgg
     2041 tgagcgaatc ggccgaggcg cgggccctgg cgaccgtcag tgaaactgaa ccagcagtaa
     2101 tcgtggcgag tagcactgtg cactcggcag acgattttgg tttgttgcga gagatgcggg
     2161 cccaacaacc ggacaccccc attatctggg tcacaggcgc cggctcagta gagatggccc
     2221 tccatgtcgt gcaagacgaa ggcgcttacc attattttga gcgaccggta gatgcggaca
     2281 agttgcgcat cgtgctcgac cgcgcggttg agttatcaga agcaaagcgg gagaacgcgc
     2341 tgctgcgccg gcagcttcag gaacgcggtg cctttggtga attggttggg cagtcggaac
     2401 agatgcggca aatctattcg cttatcgagc aagttgctcc ttcgtccgct tcagtactta
     2461 tcaccggaga ttccggcagt ggcaaggaac ttgttgcccg cacgcttcac cagaagagtc
     2521 cgcgcaaaga caagcccttc gtagcgatta actgttcggc gattcccgaa actctgatgg
     2581 agtcggaact tttcggacac gagaaaggtg cgttcaccgg ggccgcctca agaaggcaag
     2641 ggtgttttga actcgcaaat acaggaaccc tcttgcttga tgagatcgcc gagatgccgc
     2701 ctctactgca agcgaagtta ctgcgcgtga ttgaagagcg ggccatccgc cggttgggaa
     2761 gccgtagcga aatcagcatt gatgtgcggc tcttggcagc gacgaaccga gatccggcgg
     2821 acgcagttcg cagcggtagc ttgcgtgagg atctgttgta tcgcctgaat gtgtttcggg
     2881 ttcacttgcc gcccttaaag gaccgcaaag acgacttgcc gctattggcc caacaccttg
     2941 tgacgcagtt ggcggagaag cacgaacgcc cggcccgctt tcttagtcca agcgctattt
     3001 ccgcgcttca gtttcacgcc tggccgggca acgtgcgcga actgcgtaac gtgatcgagc
     3061 gtgcggttat catctgctct ggcgagcaaa tagaacggca tcacttcgcg ccctacccaa
     3121 ttgatcagcg cgagcgactg cgggatgaag acaccatctc cttccctgtg ggaacaccga
     3181 tagaagaagt cgagcgccag atgatcatgc gcacgttgca aaagactaag aacaacaaaa
     3241 cccgcgctgc ggtgctgctt ggtttcagtc ttaagacgct tcacaacaaa ctcaatctct
     3301 atcgcgagcg tggcttgctc catgatgagt aagtagcggg cggtcttgcc agtgcaaacc
     3361 cggctctgcc tatttcagaa tcttatccaa tcctctgatt aggccaacat gatttctgac
     3421 ttcgcgaata gcgaggagcg ttccctgtat gtaaggctcg gctccgcttc cagcatcatg
     3481 gcggatgctc aaacgctcat cgctggcgcc aaaaatggct tcgacgctga taacgtaact
     3541 gggtagacgc acggagtgaa tctgaattcc gtttagtgtt gtcccgcgac tctctttctc
     3601 tccgattacg tcaccaggtg gatgagtaac ttccggcttc ctgacctctg agaggcgaag
     3661 cgccagctcc cgagttgtgc cactcggggc atccttttta gcgtcggagg cataatcaat
     3721 gatctcccaa tgagacaaat actttgcggc ttcgcaggca aagcgctgca gcaacactgc
     3781 tgtaatcgca aaatttcccg cagccatcac gccaactctg gcagctaacg cagattcgtt
     3841 gatttcaaca aagtcctcat ctgtcaaacc ggaagaaccg atgaccacgt gaacaccttt
     3901 gcgtattgcg gtcatcacgt gcgctttcac gacgtctgct ttcgtgtaat ccactagcac
     3961 gtcggtgggc gtctcgaggg cttcaccgac cgatccgcta acgatcaagt caatgttcga
     4021 ggcacccagg atgtctttga ggttctcccc tctgtgggtg cg
//
LOCUS       DASUEX010000574         5082 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_530672, whole genome shotgun sequence.
ACCESSION   DASUEX010000574 DASUEX010000000
VERSION     DASUEX010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5082
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_530672"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..928)
                     /locus_tag="VFV61_09580"
     CDS             complement(<1..928)
                     /locus_tag="VFV61_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900772.1"
                     /translation="MATRILCLLTLCASALCHAHAQTNQHNSEYLGRYTDGAEYVVYF
                     EQTPYGLTLRPALWTATQLLKQTGEDQFTVTDRASRGATFHRDQRGAVVGVSIRGMEG
                     EGLTLTRASRQLPIEMLLAGDGQNAARAYLTQGGNDAASRILKAAERVLARFPTKASN
                     VVAFLSTATQSFAKDANLHTLLAYAQVASGDRKSALASFRRAYTLDPTNRNAVSGLAL
                     LQALPAPDAAKDTPWKLPFPLAAVFAPPTAGEIRAVEADWQSRDLSVRGVEEVTTGRI
                     KFEADEFTVRIVSHQVHGQRHYGAIISPVNAKP"
     gene            complement(1031..4738)
                     /locus_tag="VFV61_09585"
     CDS             complement(1031..4738)
                     /locus_tag="VFV61_09585"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX4900773.1"
                     /translation="MTPERWQQVEEIFQAALDRTPEERARFVSEACANDISLKRDVEV
                     LLSQHESAGDLLAESNLNSPLDHEEDADPMIGRRLGNYRIERELGRGGMGAVYEAARI
                     DNEFRKRAAIKLVKRGMDTDFVLRRFRKERQILAALDHPNIALLLDGGTTDDGLPYFV
                     MEYIEGQPLYRYCDAHQLTVAERLRLFLSICDALHYAHQKQVIHRDIKPSNVLVTLDG
                     VPKLLDFGIAKLLNPELVGDITHDPTVTAMRLMTPEYASPEQVQGAPTSPTTDVYSLG
                     VLLYELLTGHRPYRLRNRAPHEMARVICEETPAPPSFVISRPEDLLPKSVTGNEMTTV
                     EQVYSARNATAESLRRDLAGDLDTIVMRALRKEPERRYQTAEQLRDDISRYLEGQPIS
                     AFPESSPSYFTSGSQPITENSLAVLPLKLLDFRQGSDSGPDYLGTGLADALITRLSTI
                     RRFAVRPTSSVLRYGADADPLVAGRELGVAFVLDGRVRRAGERIRVTIQLLNVRDGTS
                     MWAAQFDEKFTDVLNLEDTISSSVAEAIIPHLTGDERLRLAKRGTDNPQAHEAYLRGR
                     YYWNTFSEEGFARAIVCYNQAIALDPNYAVAYAGVAAYHSWLGSFTVLPFAECAAAAY
                     EAASTAVTIDPMLAEGYAALGQAILCKDFDWERAERQLQTAIELNPNYSVARIWYALQ
                     LTMEGRCQEGLHQAKLARDLDPLAIISRFSLAWCSYHARRFNEGYLLAQETLATEPNN
                     LMMQHVASFLLSRLGRHDEAIAMAEKCVALMGKASHTMSRLGSALAEAGKVEEAEAVL
                     REMHEITSHRHISPYHLALVNCSLGHVEQGLELLELAYETRDAKILWMAVDPELDQLH
                     GKPRFDRLLRKLKHPLRSLSALQLPSQTGEESSFASSIPPASTNVTSPVASPAAQTLD
                     SGNEEARQLYTAGRYYATRRTAEGLWQAIDRLKRAVELDPAFALAHSELADCYSLLNW
                     YVEPPPADAWDLAKQSALNAVTADSQLAEARASLGFVRLHYDRDWEGAERELRTAIQL
                     KPGAQVAHRWYAFSLSAMGRHEEAIVEMERAREISPQSAVLATAVANVLFLAGKYDDA
                     IEQCRRSMDLDSGAVSVHTVLRWCYEKKGMHREALAVFEQERVFAGDTATTHLKRASV
                     LAAVGRNEEARAILIESIERRPKQWVSAYEIAIVYCWLGDYDNAFRWLAQAEREHAVG
                     FTFVRVDPRLEGLRSDPRFQGLLRGVDRTMP"
BASE COUNT         1132 a         1438 c         1421 g         1091 t
ORIGIN      
        1 aaggcttagc attcactggc gagattatcg cgccgtagtg gcgttggcca tgtacctggt
       61 gagagacaat gcggacggtg aattcgtctg cttcaaactt gatgcgtccg gtggtgacct
      121 cttccacacc ccggacgctg agatcgcggg attgccagtc tgcttcgacc gcgcggattt
      181 ctccggctgt gggtggtgca aaaactgccg cgaggggaaa gggcagtttc catggagtat
      241 ctttcgcggc gtccggtgcg ggcaacgcct gcaagagtgc aagtcccgaa acagcgttcc
      301 tgttcgttgg atctaaggta taagcgcgac ggaaactcgc caatgcagac tttcgatcac
      361 ccgaggcgac ctgagcataa gcgagcagcg tatggagatt tgcgtccttg gcaaaacttt
      421 gcgttgcggt tgagaggaaa gccactacgt ttgaggcctt ggtcgggaag cgtgcgagaa
      481 cgcgctcagc ggccttgaga attctgctag ccgcatcgtt gccgccctga gttagatagg
      541 ctcgagccgc gttttgaccg tcaccagcca aaagcatttc gatgggaagt tgccgggaag
      601 cgcgcgtgag agttaaccct tcgccttcca tgccgcgaat ggagactccc acaacggcac
      661 cgcgctgatc gcgatgaaaa gttgcgccac gcgaggcacg gtcggtgacg gtgaattggt
      721 cttcgcctgt ctgtttaagt aactgcgttg cagtccagag agcaggccta agggtaagtc
      781 cgtacggggt ctgctcaaag taaactacat actcggcgcc atcggtatag cgtcccagat
      841 attcggaatt gtgttggttg gtctgagcgt gagcgtgaca aagagcagaa gcgcagagag
      901 taagtagaca aaggattctt gtcgccatgc attcttagca ccgcggaagt cttatttatg
      961 acagattttt gaaggactat ccgcgaagtc agaaccagcc gcggtagcgg ataccgcaga
     1021 cggtactgac tcagggcatc gttctgtcaa cgccgcgcag caatccctgg aaacgcggat
     1081 cagagcgcaa accttccaaa cgcggatcga cgcgaacgaa ggtgaatccc acggcatgct
     1141 cgcgttctgc ttgcgccagc cagcggaaag cattgtcgta atcaccaagc caacaataga
     1201 caatggcaat ttcgtatgct gagacccact gcttcgggcg ccgctcaatc gattcgatta
     1261 agatcgccct tgcttcttca tttcgcccga ccgcggccaa cacactggcc cgtttaagat
     1321 gggtcgtggc tgtgtcaccg gcaaacacac gctcttgttc gaagacagcc agcgcttcac
     1381 gatgcatgcc cttcttctcg tagcaccaac gcagcactgt atggaccgat acggcccccg
     1441 aatccaggtc cattgatctg cgacactgtt cgatcgcgtc gtcatatttg ccggccagga
     1501 ataaaacgtt ggccactgcc gttgccagca ccgctgactg tggcgaaatc tctcgagcgc
     1561 gttccatctc tacaatcgct tcctcgtgcc gccccatagc cgaaaggcta aaggcatacc
     1621 agcgatgcgc tacttgcgcg ccgggtttca actgaatagc ggtgcgtaac tcgcgctccg
     1681 caccttccca gtcgcgatcg taatggagtc tcacgaaacc aagtgatgcc cgcgcctccg
     1741 ccaattggga atcagcggtg acggcattaa gcgcagactg ctttgcgagg tcccacgcat
     1801 ctgcgggcgg cggctcgaca taccagttga gcagcgaata gcaatccgca agctctgagt
     1861 gcgccagcgc aaatgcaggg tcgagctcca ccgcccgctt caaccggtcg atggcctgcc
     1921 agagaccctc agccgtacgg cgcgtagcgt aataacgacc agccgtatag agctgacgcg
     1981 cctcctcatt tccactatcc agggtctgtg cggcaggact tgccaccggt gacgtcacat
     2041 tggtgcttgc cggtggaatc gatgatgcga agctcgactc ctctcctgtt tgtgatggta
     2101 actgcaacgc tgagagtgac cggagcggat gttttaactt acgcagcagt ctgtcgaaac
     2161 gcggttttcc gtgtaactgg tcgagctctg gatcgaccgc catccagagt atctttgcat
     2221 cgcgagtttc gtacgcgagt tccaggagtt ccagtccctg ctccacgtgg cccagcgagc
     2281 aattaaccag cgccagatgg taaggagaaa tatggcgatg cgaggtaatc tcatgcatct
     2341 cacgcagcac tgcctcagcc tcttccacct ttcccgcttc cgccagggcc gagcccaggc
     2401 ggctcatcgt gtgagaggcc ttgcccatca aggcaacaca tttctcggcc atcgcaatcg
     2461 cctcatcgtg ccggcctagc cggctgagaa ggaaactggc gacgtgttgc atcatcaaat
     2521 tgttcggttc tgtagccagc gtctcctggg ccaacaggta gccctcgtta aagcgccgtg
     2581 cgtggtagga gcaccaggcg agactaaagc gcgagatgat cgccagtgga tccagatctc
     2641 gcgcaagttt cgcttgatgc aacccctcct ggcagcggcc ttccatagtc aattgcaggg
     2701 cataccaaat ccgggccacg gaataattgg gattcaattc gatcgccgtc tgcagttgac
     2761 gctcggcccg ttcccagtcg aaatctttac acagtattgc ctggcccagc gcggcgtaac
     2821 cttcggccag catgggatcg attgtgactg cagtcgaggc ggcctcatag gcagccgctg
     2881 cacattcggc aaatggcaaa accgtaaacg agccaagcca actgtgatag gccgccactc
     2941 cggcataagc gaccgcgtag tttggatcga gtgcaatggc ctggttgtaa caaactatcg
     3001 ccctggcaaa gccctcttca gaaaacgtgt tccagtaata acgcccgcgg agataggctt
     3061 cgtgagcttg aggattatca gttcctcgtt tggcgagcct gagccgttcg tcgccggtga
     3121 ggtggggaat aatcgcttcc gccacactcg aggagatcgt gtcctccaaa ttcagaacat
     3181 cggtgaactt ctcatcaaat tgggcggccc acatcgaggt gccatcgcgc acgttgagca
     3241 actgaatcgt cacgcggatc cgttcaccgg cccttctgac gcggccgtca agtacaaaag
     3301 caacaccaag ttcacggccc gcaacaagtg gatctgcatc ggctccataa cgcagaacgc
     3361 tactcgttgg gcgtacggca aaacgccgga ttgtgctgag ccgcgtaatc agggcatcgg
     3421 cgagtcctgt gcccaggtag tcgggtccag agtctgatcc ctgacgaaaa tccaggagtt
     3481 tgagtggcag caccgccagt gagttttccg tgatcggttg tgaaccactg gtgaaatagc
     3541 tcggggaaga ctccgggaaa gcagaaattg gttgtccttc gaggtagcgc gagatgtcat
     3601 cacgcaactg ctcggcagtt tgatagcgcc tttccggttc cttccgcagc gcacgcataa
     3661 cgatcgtgtc aagatcgccg gcaagatctc gccgcaaaga ctcagccgtt gcgtttctcg
     3721 ccgaataaac ctgttcgaca gttgtcattt cgttccccgt caccgatttt ggcaagaggt
     3781 cttcggggcg actaatgaca aaactgggtg gcgccggcgt ctcttcacaa atcacccgcg
     3841 ccatctcatg gggcgcccga ttgcgcagtc gatagggacg atgtccggta agcaattcat
     3901 aaagcaacac gccgagtgaa tagacgtcgg tggtgggtga agttggagca ccctgtacct
     3961 gctcaggcga ggcatattcc ggtgtcatca aacgcatcgc ggtaacggtg gggtcgtgtg
     4021 taatgtcacc aaccaattca ggattgagca gcttggcaat gccaaagtcc aacagctttg
     4081 gtacgccgtc aagcgtaacc agcacgttcg atggctttat atcgcggtgg ataacctgtt
     4141 tttggtgggc gtagtgcagg gcatcgcaaa tcgacaggaa aagtcgaagc cgctcagcga
     4201 cggttagctg atgcgcatcg caatagcggt agagcggctg cccttcaatg tactccatca
     4261 caaagtacgg caggccgtcg tcggtggtgc ctccatccag cagcagcgcg atgtttgggt
     4321 ggtcaagggc ggcgagaatc tgccgctcct tgcgaaagcg gcggaggaca aaatcagtgt
     4381 ccataccgcg cttgaccaac ttgatggccg cacgctttct aaactcgttg tcgatgcggg
     4441 ctgcctcata gaccgccccc atgccaccgc gtccgagttc gcgctcaatc cggtagtttc
     4501 ccagccgccg gccaatcatc ggatcggcgt cttcttcatg atccagtggc gagttgaggt
     4561 ttgattcggc gagaagatcg cccgcactct catgctgcga cagcaatact tccacatcac
     4621 gcttcagcga gatgtcattc gcacatgctt ctgacacgaa ccgggcccgc tcttcgggcg
     4681 tacggtccag agccgcctga aatatttcct ccacttgttg ccagcgttcc ggggtcatgg
     4741 gggcgaattc acacgagcgg tgacacctat actcggtggc agctagaagt tcgttgcggc
     4801 gatatcagtc cgttgatatc agtccgttga tcatatcgat cgagagccgg ggcaccattc
     4861 tttagtatcc ggttagcact aaatagtttg cagaaagttt cgaaactcaa tcgtaagtga
     4921 cgggtagtaa aagatcaaac gccaacgttt tgattcctcc actccctgca gaagtagcaa
     4981 tgaactcaca caaaaatttc acgaacggtc ccggcacccg gaacgcagaa acgcctgcaa
     5041 gtcgataatt caacttacag gcgtctcgcg aggagagggt tt
//
LOCUS       DASUEX010000575         5730 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1353835, whole genome shotgun sequence.
ACCESSION   DASUEX010000575 DASUEX010000000
VERSION     DASUEX010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5730
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1353835"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(170..1735)
                     /gene="nrfD"
                     /locus_tag="VFV61_09590"
     CDS             complement(170..1735)
                     /gene="nrfD"
                     /locus_tag="VFV61_09590"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEX4900774.1"
                     /translation="MLEPQQIKWAKVIDNTRCIGCHACTTACKSENIVPLGVTRTYVK
                     HVDIGTFPQARRAHQVTRCNQCAHAPCVTACPTTAMFKRADGIVDFDKSICIGCKACM
                     AACPYDAIFINPEDHSAEKCNFCAHRIDIGLEPACVVVCPTQAILVGDMNDPTSYVAQ
                     IVNRESVNVRRPEKETLPKLFYKGGHQATLDPLAARRPDNGLFMWSEQLEDSTHVVSG
                     NPNYNNSSAAALLSYDVAHSIPWDWKVSLYTWTKGIAAGAYLVPLLLVLFGYLRQESV
                     IWVWVAPVLAGVFLAVTGGLLLLDLEHPERFFLIFTRPQWRSWLVKGAFIIAAYTLVL
                     ALHFSASWIGAFYLHRWLMIPGLPLAILTAVYTAYLFAQAKARDMWQNPLLPPHLLVQ
                     AMLVGSAALLPFVVRFEPETRLHAGITTPLLWILALTSLLHALMIWGEISLTHATAHA
                     RLAIWEMVQGRYKSDFWIGVILSLLGGLLPLLALLGYVSTALGVGGAPMALIGLMLYE
                     NAYVQAGQSVPLA"
     gene            2010..2357
                     /locus_tag="VFV61_09595"
     CDS             2010..2357
                     /locus_tag="VFV61_09595"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX4900775.1"
                     /translation="MRIFSSLAILVLAIAAAVLFGACNSTERTASKVAPTANPAAPNK
                     INPAPADTARRVNVKEAQDLIASGRAVVVDVRNQAAFDQGHIRGAKLIPFAEVADRSG
                     ELPRDKTIITYCS"
     gene            complement(2703..3677)
                     /locus_tag="VFV61_09600"
     CDS             complement(2703..3677)
                     /locus_tag="VFV61_09600"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX4900776.1"
                     /translation="MLDLPAPMFPFRNILFPTDFKPHTRAALKYAAAFARRGDGRVVV
                     LSVQGAKVPANLLAQSEPIAENEDRWISQLRDDFRDVLADPLFKELDVKPVIVAGEPA
                     SEIARAAQDYGSNLVTIVTHGRKGLTRALWGSTAEEIIAEAPCPVLTIRPPQHDFVEH
                     DGNSCQLRLNRILLATNFRPSSSAATQVATQLANQTGAELHAIHVIGDYFEQLAELFP
                     EGGLSALKRLRGYAQERMAQLARGDGARAITHITEGRPYEEVVRLASASEVDLIVIGT
                     NVHASIFGGTPVLGSEIERVVRNAPCPVLCVPASKAMTPLPAFVAQPV"
     gene            3925..5661
                     /locus_tag="VFV61_09605"
     CDS             3925..5661
                     /locus_tag="VFV61_09605"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX4900777.1"
                     /translation="MLKGLFRTKSLDDILAGIGGPQFALRRTLGAFNVTLLGIGAIIG
                     AGIFATIGTAAAGDAARPGAGPSLMLSFVITAIVCAFTALCYAEFASMVPISGSAYTY
                     SYATLGELVAWIIGWDLIIEYAVGNVAVAISWGNYFRTLLSDLGIDFPLWLATDYRTA
                     AKIPGLYESAPHIFGIPIVFNVLALGIVALITIVLVWGIRESAGFNAVMVCIKILVLL
                     FFIVVALYFVSPGGMAKNWAPFQPNGWKGTFTGAALVFFAYIGFDAVSTVAEETKNPS
                     RDLPIGIIASLVICTFFYVLVAAVFTGIMPYNELVQRLATEQAEPLTMALNHVAPDAN
                     WASGIVAFGSVVAHTAVLLVFQLGQPRIFFSMARDGLLPPVFASVHPKYKTPHVTTIL
                     TGVVVGGFAAVMSIDEMVDLTNIGTLFAFVLVCVGIIILRHKDPGRVRPFRVPFGTWL
                     IPSLGAISCIFLMYYLPPASWWRFIGWLMLGMSIYLSYGYVRSTVGQNIGRPFPTPLG
                     LKVAAVGFLLLAVGLFIIPHSSTTVELYEKMMSGVAEGKRTIVAFSSIGIGALLAVAG
                     LLFGSGHTATNK"
BASE COUNT         1276 a         1604 c         1573 g         1277 t
ORIGIN      
        1 cggtagaggt cgtcgattgt cgcttcagtt ggaatactca tgaggtaact tcagctccta
       61 acagagttga ttctaatgct ctcaaaagaa gatgacgtca aggacgcccg ccaatcttcg
      121 ccgcagaaat cgatacaaga atcccagctc acaatcactc gtccgattgc tacgccagcg
      181 gcactgactg ccccgcctgc acatacgcat tctcgtaaag catcaatccg attaaggcca
      241 ttggggcgcc accaactccg agggcggtgc tcacgtaacc cagcagggcg agcaacggca
      301 gcaaaccacc aagcagcgag agaattactc cgatccagaa gtcgcttttg taacgaccct
      361 gcaccatttc ccagattgcg agtcgcgcgt gcgccgtcgc atgggttaac gatatttctc
      421 cccaaatcat tagtgcatgc agcaagctgg tgagggcgag aatccacaac agcggtgtgg
      481 tgatacccgc atgaagtctc gtctccggtt cgaagcgcac gacaaacggc agcagcgctg
      541 ccgaaccaac gagcatggct tgaacgagaa gatgcggcgg cagcagcggg ttctgccaca
      601 tgtcgcgcgc ctttgcctgc gcgaaaagat atgcagtgta gaccgccgtt agaatggcca
      661 gtggcagtcc cggaatcatc agccagcgat gcagatagaa tgcgccgatc cagctcgcgc
      721 tgaagtgcaa tgcgagcaca agcgtgtacg cagcgataat gaaggcgccc ttcaccagcc
      781 agctgcgcca ttggggccgc gtgaagatga gaaaaaaccg ctccggatgc tccagatcga
      841 gaagcagcaa cccaccggta accgccagga aaacgcccgc gagcaccggc gcgacccaaa
      901 cccaaatcac actctcctga cgcaagtaac caaacaacac gagcagcaac ggaacgagat
      961 aagctccggc ggcaattccc ttagtccagg tataaagact gactttccag tcccagggaa
     1021 tcgaatgcgc aacatcgtat gagagcaatg ccgctgccga ggagttgttg tagttggggt
     1081 ttcctgaaac tacgtgggtg ctgtcttcca gttgttcact ccacatgaac aaaccgttgt
     1141 cgggacggcg cgcggccagg ggatcaagcg tcgcctggtg ccctcctttg tagaaaagct
     1201 tcgggagggt ctctttctcc ggacggcgca cgttaaccga ctcgcggttg acgatctgcg
     1261 ccacatacga ggttgggtcg ttcatgtcgc caaccagaat cgcctgggtg ggacaaacga
     1321 cgacgcaggc aggttcgagt ccgatatcga tgcggtgcgc gcagaagtta catttctccg
     1381 ccgagtgatc ttccggattg atgaagatag cgtcgtaagg acaagcagcc atgcaggcct
     1441 tgcagccaat gcaaatcgat ttgtcgaaat cgacgatgcc gtcggcgcgc ttgaacattg
     1501 cggtcgtcgg acaggcggtg acacacggcg catgcgcgca ttgattgcag cgcgtcacct
     1561 gatgcgcgcg gcgcgcctgg ggaaacgtgc cgatgtcaac gtgcttgacg taagtgcgcg
     1621 tgacgccgag gggaacaatg ttttcagact tgcaggcagt cgtgcaggca tgacaaccaa
     1681 tgcaacgcgt gttgtcgatg accttggccc actttatctg ctgcggctca agcatgagtt
     1741 tggttaagta atgaaggggc agactataac acgattggcg atggccaatt gccgattggc
     1801 gattggcgtc ggagccgaag tgaggtcatt accaccaggc ggtagccggt ggccgagcga
     1861 ttaccaattg gcacgtatcg aaagtggcca atcggcaatt ggaaattggc aatcggcaat
     1921 gtgattgatt gacaaaaact ctctgccata agaaattaga tacgacgttt ggttcgaccc
     1981 ttcagttgtg aatcgttctg gagtaattca tgcgaatttt tagttcactt gcaattctag
     2041 tcctggcaat cgcggccgca gtcctattcg gggcttgtaa ttccacggag cgcacggcgt
     2101 caaaagtcgc cccgactgcg aatccagcgg ccccgaacaa aatcaatccg gcaccagccg
     2161 acaccgcccg ccgcgttaat gtgaaggaag cgcaggacct gattgccagc ggcagggccg
     2221 tggtcgtcga tgttcgcaat caggcggcct tcgatcaggg tcacatccgc ggtgctaagt
     2281 tgattccgtt cgctgaggtt gccgatcgca gtggggagct gccacgcgat aagacaatca
     2341 tcacctactg ttcttgacca aacgaacaca cgtccgccgg tgcggtggac ataatgaaaa
     2401 caaaaggaat tcaaaacgca gcagcgttgc taggtggttt tgacggttgg aaacaggcgg
     2461 gtttgccggt cgactcgaaa cagtagccgc cgcaagtctc aggtctcagg tctcaggtct
     2521 caagtctcaa gtctcaagtt tcaggtctca ggtctcaggt ctcaagtctc aggtctcaag
     2581 tttgacgttc ggggaagcat cacgaaacag actcgaaatt tgagacgaga acttgagact
     2641 cgtaacttga ggtctgagac ctgagacttt ggactttggg ctttggactt tggactttgt
     2701 ttttaaaccg gctgagctac gaatgccggt aacggtgtca tggctttgct cgcgggtacg
     2761 cagagcaccg gacagggtgc gttacgtact actcgctcga tctcggaacc aagcaccggc
     2821 gttcccccga aaatcgaggc atgaacgttc gtgccaatca cgatcaaatc aacctcactc
     2881 gctgacgcca gccgcactac ttcttcatat ggacgtcctt ccgtgatgtg tgtaatcgcg
     2941 cgtgcgccat ctccgcgcgc cagttgcgcc atccgctcct gcgcgtaacc acgcaggcgc
     3001 ttcagcgccg aaagcccgcc ctcaggaaac aattcagcga gctgttcgaa gtaatcgccg
     3061 atgacgtgaa tcgcatgcag ctcggcgcca gtctgattgg caagctgagt agcaacctgg
     3121 gttgccgcac tcgacgaagg acgaaaatta gttgccagca aaatgcgatt cagacgcaac
     3181 tgacagctat tgccgtcgtg ttcgacaaag tcatgctgcg gcgggcgaat agtcagaacc
     3241 gggcaaggtg cttccgcgat gatctcttca gcagtcgaac cccaaagcgc acgcgtcagt
     3301 cccttgcgcc cgtgcgtcac aatcgtaacc agattgctac cgtagtcttg cgcggcgcgg
     3361 gcaatttcgc tggcaggttc gccggcgaca atcaccggtt tgacgtccag ttccttgaag
     3421 agaggatccg caagcacatc gcgaaagtca tcacgcagtt gggagatcca gcggtcttcg
     3481 ttttcagcaa ttggctctga ttgcgccagg aggtttgcgg gcacctttgc gccttgcacg
     3541 ctcagaacca cgacccgacc atcgcccctg cgcgcaaatg cagcggcata cttcaacgcc
     3601 gcgcgcgtgt gtggtttgaa atcggtggga aacaagatgt tgcggaaggg aaacatgggc
     3661 gcaggcaagt ctaacaagaa ggctggactg gtaactagtc ttttgcgatt gccaagtgcc
     3721 aattgccgat tgccaactcg aacaactgag gtcaaatgcc agttgtaatc taatgccccg
     3781 atttcccaga gccctaatgg aaaatctcaa tcggtaattg gcaatggcct tttgacgtgg
     3841 gacacaaata tgcgaaactc cgccagcatt ccttagctat agcaagcgca agacctatct
     3901 cgttcgattt aggaggcgac tctcttgctc aaaggactct tccgcacaaa aagtcttgac
     3961 gatattctgg cgggcatcgg cgggccgcaa tttgcactga ggcgcaccct cggtgccttc
     4021 aacgtaacgc tgctcgggat tggcgccatc atcggcgccg gaatcttcgc caccattggc
     4081 accgccgccg ccggcgatgc agcacggcct ggcgccggcc cttcgctgat gctgtcgttt
     4141 gtgatcacgg caatcgtttg cgccttcact gcgctctgct atgccgagtt tgcttcgatg
     4201 gtgccgatct ctggatctgc ttatacctat tcctatgcca cgctcggtga actcgtcgcc
     4261 tggatcatcg gctgggactt aatcatcgaa tatgcagtag ggaatgtcgc cgtagcgatt
     4321 agttggggca actacttccg caccctgctg agcgatctcg ggatagattt cccgctctgg
     4381 ttggctaccg actaccgcac cgctgccaag attcccggcc tttatgaaag cgccccgcac
     4441 atctttggta ttccgatcgt gttcaatgtg ctggcgctgg gtatcgtggc gttaattacg
     4501 attgtcctcg tctggggtat cagagaatct gccggattca atgccgtaat ggtctgcatc
     4561 aagatcctcg tcctgctctt cttcatcgtc gttgctcttt atttcgtctc gcctgggggc
     4621 atggcaaaaa actgggcgcc gtttcaaccc aatggctgga agggaacttt caccggcgcg
     4681 gccttagtct ttttcgccta catcggcttt gacgccgttt cgacggttgc ggaggaaacc
     4741 aaaaatcctt cacgggatct acccattggc atcattgcct cgctcgtaat ctgcaccttc
     4801 ttttacgtac tggtggccgc agtcttcact ggcatcatgc cttacaacga actagtgcaa
     4861 cgactggcca cggagcaagc agagcctttg actatggcac tcaaccacgt tgcgcctgat
     4921 gcgaactggg ccagcggcat cgtcgccttt ggttccgtcg tcgcacatac agccgtcttg
     4981 ctcgtgttcc aactgggtca gccccggatc ttcttttcaa tggcacgcga tggactgctg
     5041 ccaccagtgt tcgcgagcgt gcaccctaaa tacaagaccc ctcacgtaac tactattttg
     5101 accggcgtcg ttgtcggcgg ctttgcggcc gtcatgagca tcgatgagat ggtagacctc
     5161 acaaacatag gcaccctctt cgcgtttgtc ctcgtgtgcg tgggaattat catcctgcgt
     5221 cataaggatc ccggacgagt gcgacccttt cgtgtgccat ttggcacgtg gctaatcccg
     5281 tcgctcggcg ccatctcctg cattttcctg atgtactact tgccgccggc ttcctggtgg
     5341 cgcttcatcg gctggctaat gctgggaatg tcgatctatc tctcttacgg ttacgtgcgc
     5401 agtaccgtgg gccaaaacat cggcaggccg tttccaacgc cgctgggttt gaaggttgcg
     5461 gctgtcggat ttctactttt ggccgtcggc ctcttcatca ttcctcacag ctcaactaca
     5521 gtcgaactct acgaaaaaat gatgagcggt gttgccgagg gcaagcgcac gattgtggca
     5581 ttcagcagca ttgggatcgg tgcactgctg gcagtggccg gtttactttt cggatccggg
     5641 cacacggcga cgaacaagta actttgcgca gactgatttg agatcaagtt aacggagagc
     5701 actggagcgt gacgcttact ctactggcca
//
LOCUS       DASUEX010000576         5660 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1689253, whole genome shotgun sequence.
ACCESSION   DASUEX010000576 DASUEX010000000
VERSION     DASUEX010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5660
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1689253"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..731
                     /locus_tag="VFV61_09610"
     CDS             <1..731
                     /locus_tag="VFV61_09610"
                     /inference="COORDINATES: protein motif:HMM:NF020260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900778.1"
                     /translation="EVVAGVSPTTGEGIAGVSPALSAQREPIPSQLPPLNSAEKFARE
                     VPVESLSNNIRPLGQLDESFIIATDDEGLLLIDQHVAHERILFDKYRALESQRTAESQ
                     QLLLPETFDLTPAQAAAFEVVAEELEKYGFDLMRLSGRTVAIKATPADLPANEARNML
                     AEVLDTVDAEKQGAARETLRDEIAASLACHAAIKVNMPLAPEKMRWLIDRLLLTSSPT
                     TCPHGRPVILRLATKDILKGFHRI"
     gene            801..1541
                     /locus_tag="VFV61_09615"
     CDS             801..1541
                     /locus_tag="VFV61_09615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900779.1"
                     /translation="METKDILTFGLALLAFIVSAISTTITIVRGRREKQRAIRNEITN
                     VLSQIVATAIENAKLYQENNEKPTPYYQAVSSILNQRNAFLLNQAVYLSEQVPQLITA
                     VEYNTLAAANANAGDLFTSEKFYRKAIERSNNPFYRSMALRSYAGFLFPQRRFTEARE
                     IFRQAINLMQGNGDQVHYTNGFTYQMWAWNELVNAEAIELARQMFESAENEFRKIDND
                     KVGRDAIMGLYAAVPLPPTTSHSVTVQG"
     gene            complement(1669..3855)
                     /locus_tag="VFV61_09620"
     CDS             complement(1669..3855)
                     /locus_tag="VFV61_09620"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX4900780.1"
                     /translation="MKRLSPLLTLALTLIFTLCAQPILVAQVANPTNGHFQGAGRYEK
                     QIRLFDDFARKQMALDKTVGMTIGFMKDDFVWVKGYGYADLENKSPAKAESAYRLASV
                     TKPMTAVAILQLVEKGKIDLDAEVQTYVPYFPRKPWPVTVRQVLGHLGGISHYKNPAN
                     ELHIKEHRSTREAIAIFENFDLVAEPGTRYSYSSYGYNLLGAIVEAASGMSYGDYMRQ
                     NIWQPLGMTETRMDDPLEVIPNRVRGYQLINGQVKNSEFIDISSRFAAGGTRSTVPDL
                     LKFAKGIIEDKALKHDTLNMMATSMSTRAGRLTNYGMGWETGPANGRYMLVHSGGQQE
                     TRTLLYILPTRKMALAVAVNFEGSNPGVYLDRFFQLLTGSPLILNLYSSDKVKAAASE
                     AIKATFDYGLSDFERTQKSLPASDKELVEAFAYFNSLVNPETLKANSPDTLAKIRQGA
                     HPVAKQAFTKIGAFMAQKLGGKKGASGLDRYAAQGGLAFFQDYITLTKSDASTPKDVQ
                     FNEPFAALVTEMARDWNKSNTDYVRQLWLMPDTDFAALSKTLSQSFAGATVYPNLVDD
                     LFNVTRQFILNRDGARALQASKLAVELYPDSPHANFIEGISWIANKDTARGLASLKKS
                     ATLNPAPNGPASAGGLNNVAYQLAGVGRVDEAMVILQNAIEMYPQEANLYDSLGEFQL
                     KKGEKAKALASYQKALTTNPEFGNAAAAKEIVKKLTEELAANPARP"
     gene            complement(4201..>5660)
                     /locus_tag="VFV61_09625"
     CDS             complement(4201..>5660)
                     /locus_tag="VFV61_09625"
                     /inference="COORDINATES: protein motif:HMM:NF014722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein adenylyltransferase SelO family protein"
                     /protein_id="HEX4900781.1"
                     /translation="SVWNGYLRTGALIFDISSRGTGATILSPGAQEADGAVETGNEIY
                     GYSSGLAELDEMVGSAVMSEIFYRQGIPTERCLAVIGFTDGSAIGVRSAPNLIRPAHI
                     FRYLKQGRHAELKASVDYFIRREVENHFWKIPVSSPTKYERALDYLAGSYGKMAAVLE
                     EEYIFNWLAWDGDNMLASGAILDYGSIRQFAAKHDKYRFKDVDRYSASLAEQRYWARL
                     IVQTFAQAMDFISSGKKRNLREFKTAKALQTFDSAFADESDRRILWRIGFTAEQIDQL
                     LKGARAQVTQFHKSLTYFEDLKVARGIEKLHDGFTHKPVFLIRNLLRQLPAYYLAQSL
                     NREDDESGYMPDEIFLRVMAASYVGKRDLQLTPSRRAHVRNFQQCYLRLIGALGTPFD
                     ETLKTLQERSAVINHRHRLTGDALIYIIEEVIAIKGKIEVNGLQEALDAFIDSQVLVP
                     GSWQPVDPEQLKPKTLKSRLLLTIQENLEAYKESI"
BASE COUNT         1298 a         1533 c         1476 g         1353 t
ORIGIN      
        1 cggaagtcgt cgcgggcgtc tcgcccacaa ccggcgaagg gatcgcgggc gtctcgcccg
       61 cactgagcgc gcagcgcgaa ccaattccct cacaactccc cccgctcaat tcagctgaaa
      121 agtttgcccg tgaagtcccg gttgagtcgt tatcaaataa cattcggccg ctcggccagc
      181 ttgatgagag ttttatcatt gccaccgatg acgaaggcct gttgctgatc gatcagcatg
      241 tcgcccacga aagaatcctc ttcgataaat accgggcgct ggaatctcaa cgcacggccg
      301 agtcgcagca actactactt ccggaaactt ttgatctcac gcctgctcag gctgccgctt
      361 tcgaagttgt ggctgaggaa ttggaaaagt atggttttga tctgatgcga ctctcaggca
      421 ggaccgtggc gatcaaagca acgcctgctg atttgcccgc gaatgaagcg cgcaacatgc
      481 tcgccgaagt tctcgatacc gtcgatgcgg agaagcaggg agctgcgcgt gagacgctgc
      541 gcgacgagat tgccgcctcg ctggcgtgtc acgcggcaat caaagtgaac atgccactcg
      601 ccccagagaa gatgcgctgg ctaattgatc ggttgttgtt gacttcatca ccaacgacat
      661 gccctcacgg acgcccggtg attctgcgcc tcgctacgaa ggatattctc aaaggcttcc
      721 atcggatcta acgaggaaca cgccgagcag cttgttcgcg ggctggtttt ttaccctcaa
      781 ccctcgcagg agaattcgtc gtggaaacta aagacatcct aacctttgga cttgcgctgc
      841 tggcattcat cgtatcggcg atctcgacca ccataacgat cgttagagga cgccgcgaga
      901 agcaacgcgc cattagaaat gaaattacga atgtactcag tcagatcgtc gcgaccgcca
      961 tcgaaaacgc gaagctttat caggagaaca acgagaaacc gactccctac tatcaggcgg
     1021 tctccagtat tctgaaccag cgaaacgcct tcctgctaaa ccaggcggtc tatctgagcg
     1081 aacaggttcc acaacttatc actgctgtcg aatacaacac actggctgcc gctaacgcca
     1141 atgcggggga tctttttacg tcagagaagt tctatcggaa ggcgattgag agatcgaaca
     1201 atccgttcta cagatcaatg gctctccgca gctatgcagg ttttctgttc ccgcaacgtc
     1261 gttttactga ggctcgcgag atctttagac aagcgatcaa cctaatgcag ggcaacggcg
     1321 atcaggttca ttacacgaac ggattcacat accaaatgtg ggcctggaac gaattggtta
     1381 atgcagaggc gatcgagctg gcgagacaga tgttcgagag cgctgaaaac gaattccgca
     1441 agatcgacaa cgataaggtt ggcagggatg ccatcatggg actctacgcc gctgtgccgc
     1501 tccctcctac aacctcacac tcagtcacgg ttcaggggta atgactctga ctactgaatc
     1561 gcaaaattag gaaggcaata tcttcgttgt ccagggccca acgttcaacg tctcggtggc
     1621 aacctggact ttgaaaattg gacattagac gtgggacttg tcttctcgct acggcctcgc
     1681 cggatttgct gccaattcct ccgtcagttt cttgacaatt tcctttgccg cagccgcatt
     1741 accgaactca gggttagtcg tcagcgcttt ttgataagac gccagtgcct tggccttctc
     1801 gcccttcttc agttgaaact caccaagact gtcatagagg ttagcttcct gaggatacat
     1861 ctcgatcgcg ttctgtaaaa tcaccatcgc ctcgtcaacc ctacccacgc ctgccaattg
     1921 ataagcgacg ttgttcaaac cacccgcact tgccggtcca ttcggtgctg gattgagtgt
     1981 tgctgacttc ttcagggaag cgagtccgcg tgccgtatct ttgttcgcaa tccaggaaat
     2041 tccttcgata aaattcgcgt gtggggagtc aggataaagc tccaccgcta acttactcgc
     2101 ctgcagcgcc cgcgcaccat ctcggttcag aataaactgt cgggtgacat tgaagaggtc
     2161 atcgacaaga tttggataaa ccgtcgcacc ggcgaaactt tggctcaatg ttttgctgag
     2221 agcagcaaaa tcagtgtccg gcatcagcca caactgccgc acgtaatccg tattcgactt
     2281 gttccagtcg cgtgccatct ctgtcacgag cgccgcaaat ggctcgttga attggacgtc
     2341 ttttggagtt gaagcgtcgc tcttggtaag cgtgatgtag tcctggaaaa aggcgagccc
     2401 gccttgggcg gcgtagcggt cgaggccgga cgcacctttt ttccctccca gcttctgggc
     2461 catgaaagcg ccgatcttgg tgaacgcctg tttcgccacc ggatgcgcac cctggcgtat
     2521 cttcgcgagg gtatcgggcg agttcgcttt gagagtctcc gggttgacca agctgttgaa
     2581 gtaggcgaac gcctcgacaa gttctttgtc acttgccggc aatgatttct gcgtacgctc
     2641 gaaatcagaa agcccgtaat caaaagtcgc tttgatggcc tcggaagccg cagccttaac
     2701 cttgtcggaa gaatagaggt tcaaaatgag aggcgaccct gtcagtagtt gaaagaatcg
     2761 atcgagatac acgccgggat tggagccttc aaagttcact gcgaccgcga gcgccatttt
     2821 ccgtgtcggt aaaatgtaga gcaacgtccg tgtctcttgt tgcccgccgg agtgaaccag
     2881 catgtagcga ccgttagcgg gtccagtttc ccaacccatt ccatagtttg tcaggcgtcc
     2941 agcacgtgtg ctcatggaag ttgccatcat gttcagagtg tcgtgcttca atgccttgtc
     3001 ctcgataatt cctttcgcaa acttgagcag atcgggaacg gtggaccggg tgccaccggc
     3061 ggcgaagcga ctgctgatgt cgatgaattc ggaattcttc acttgcccgt tgatgagctg
     3121 atatccgcgc acgcgattcg gaataacttc gaggggatca tccatgcgcg tctccgtcat
     3181 gccgagtggc tgccagatgt tctggcgcat gtagtcgcca taggacatgc cgctcgcggc
     3241 ttcgacaatc gcgccgagca ggttgtaacc ataactggaa tagctgtagc gcgtgccagg
     3301 ttccgccacc aaatcaaagt tctcaaagat cgcgattgcc tcacgagtcg agcggtgttc
     3361 tttgatgtgt aactcgtttg ccggattctt gtaatgactg atgccgccaa gatgtcccaa
     3421 cacctgacgc acagtgacgg gccagggctt cctgggaaag tagggcacgt aagtttgcac
     3481 ctcggcatcg agatcgatct ttcccttttc caccaactgc aggatagcga ctgccgtcat
     3541 cggctttgtt actgacgcga ggcggtaggc cgattcggct ttggccggcg acttgttttc
     3601 caggtcggcg tatccgtagc ctttcaccca cacaaaatca tccttcataa agccgatcgt
     3661 catccccacc gtcttgtcga gggccatctg tttccgggca aaatcatcga aaagccggat
     3721 ttgcttttca taccggccag ctccttgaaa gtgtccatta gtggggttgg caacctgcgc
     3781 aacgagaatt ggttgggcgc acagtgtgaa gatcaaagtg agcgcgagtg tcagcagggg
     3841 cgaaagtctt ttcatcgaaa cctccggcag atattgctga gaagtgtttt ggagtgcgcc
     3901 ggcagagtcc cggcgcgtcg agaacgacgg cgctttgggt ttacatgcaa ctgaaaagtt
     3961 ggatctcttc aacgaataga cacagtaacc tggcagtttg tggcaaaaac ctggtgggcg
     4021 acgcggcaga gatatatcac agagtcacca agacgcagcg acggaaagtg tttatgagga
     4081 taaaccctgc cttattcgga cgctgtcttt tattccactc tgttggtgac ttcgtaaacg
     4141 gtggcttgtt ttacttcgtg tctcgccgtc ttggcgtctc ggcggtcgtt tcgcagcgct
     4201 tcagatcgac tctttgtatg cttccaggtt ctcctggatc gtgagcagca gccgggattt
     4261 taaggtcttg ggcttgagct gctcagggtc cactggttgc caactacctg gaaccaacac
     4321 ctgggagtcg atgaatgcat cgagggcttc ctgtaaaccg ttgacttcga tctttccttt
     4381 gatagcaatc acctcttcga tgatgtaaat aagcgcgtcg cccgttaaac gatggcgatg
     4441 gttgattacg gctgaccgct cctgaagtgt tttcaaggtt tcgtcaaacg gtgtacccag
     4501 tgctccaatc aaccgcaggt agcactgctg aaaattcctg acatgagcgc ggcgcgatgg
     4561 cgtcaactgc aaatctctct tcccgacgta agacgcagcc attactcgca ggaagatttc
     4621 gtccggcatg tagccgctct cgtcatcctc acggttaagg ctttgtgcga gatagtaagc
     4681 gggtagttgc cgcagcaagt tacgaatgag aaatacaggc ttgtgcgtga aaccgtcgtg
     4741 cagcttttcg atgccgcgag ctaccttaag atcctcaaag taagtcagag atttatgaaa
     4801 ctgcgtgacc tgggcgcgcg cgccttttaa tagctgatcg atctgctcgg cagtgaatcc
     4861 tatccgccag aggatgcgac ggtcgctttc atcagcaaaa gcggaatcga aggtctgcaa
     4921 agctttcgca gtcttaaact cacgcaaatt ccgtttcttc ccagaagaaa tgaaatccat
     4981 agcctgtgca aacgtctgca caattagccg ggcccagtag cgttgctcgg caagcgatgc
     5041 tgagtagcga tctacatcct tgaaacgata cttatcgtgc ttggcagcaa actgccgaat
     5101 agatccataa tcaaggatcg cgccgcttgc gagcatgttg tcgccatccc aggccaacca
     5161 gttaaagatg tattcctcct ccagcacggc tgccatcttt ccgtatgagc ctgccaggta
     5221 atccagggct cgctcgtatt ttgttggact cgatactggt atcttccaga aatggttctc
     5281 aacttctcgc cggataaagt aatcgactga tgctttgagc tctgcgtgcc ggccctgttt
     5341 gaggtaacga aagatgtggg ccggtcgaat cagattgggt gcgcttctga caccaattgc
     5401 actgccatcc gtaaaaccaa tcacggccag acagcgttca gtgggaatgc cttgccggta
     5461 gaaaatttct gacatcacgg cgctacccac catttcatca agctccgcca ggccggagga
     5521 atagccgtaa atctcattgc cggtttcgac agctccgtca gcttcctgtg caccgggact
     5581 tagaatcgtg gccccggttc cgcgactgga aatatcgaaa atcaacgcac cggtccgcaa
     5641 atatccattc cagacacttc
//
LOCUS       DASUEX010000577         2355 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_99793, whole genome shotgun sequence.
ACCESSION   DASUEX010000577 DASUEX010000000
VERSION     DASUEX010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99793"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1664
                     /locus_tag="VFV61_09630"
     CDS             <1..1664
                     /locus_tag="VFV61_09630"
                     /inference="COORDINATES: protein motif:HMM:NF013570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HEX4900782.1"
                     /translation="FPELETSRDFIQRMVKLEEERFSSTLTVGLQKLDRLLDSVVGAT
                     PPAQELARIYDTFGVPRDLIRVSLIERGQKFAEDESAFNQLFDDALRQLQQGGTRAAG
                     EVQDKAKAVYSTIAQRVPRTVFGGYEGTRSENSHVLALIKDKNEVPELRAGDMGEVVL
                     DQTPFYAESGGQVGDVGRLFGHGEDESTAAVENTYSPAQGLIVHQVRVEKGALKIGHT
                     VTAAVDALKRDATRRNHTATHLMHAALREVLGTHVKQAGSVVAPTYLRFDFTHYQPLT
                     QTEIDEIENLVNYHILRNEPVQTNEMAVEEAMRSGAMALFGEKYGERVRVLSINGAEG
                     IFSKELCGGTHVRATGDIGLFKIVSDESIASGVRRIRAVTGVDAFERFRETEAIIDKL
                     TGDLRTSRSELPGVVVRLQEELKKVRREADDLRLKIATGAAGGSSNGDVAREIAGVRV
                     LAQEASDLDAAGMRQLSDTLLARLKSGVVVLGRSGDGKVALIVRTSADLTKRVPAGQV
                     IKELAPLVGGKGGGKPDMAEGGGTQPENLPKALEASYGIVERLLG"
     gene            complement(1815..2153)
                     /locus_tag="VFV61_09635"
     CDS             complement(1815..2153)
                     /locus_tag="VFV61_09635"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HEX4900783.1"
                     /translation="MKKLLAVALSLLFVSSLGVAGSAATGSTTGQSASSRRRGPVFRA
                     TKDQINQAQGILKNRGFYAGEQSGKLDDATRDGLKKFQAAENIKVTGTLNKLTLEKMG
                     IELTDKQKMM"
BASE COUNT          533 a          598 c          702 g          522 t
ORIGIN      
        1 cttttccgga actggagacc tcccgcgact tcattcagcg gatggtgaaa ctggaagagg
       61 aacgttttag ttcaaccctg acggtcggct tgcagaaact ggatcgtttg ctggattcgg
      121 ttgtgggtgc cacgccgcca gcacaggagc tcgcgcgtat ctacgataca tttggggtgc
      181 ctcgtgatct catccgcgtg agcttgatag agcggggaca aaagtttgca gaagatgagt
      241 cggcttttaa tcagctcttt gatgatgcgc tgcgccaatt gcagcaaggc ggaactcgcg
      301 ccgcaggaga ggttcaggac aaagcaaagg cagtttattc aacaatagcg cagcgtgtgc
      361 cacgaacagt tttcggtgga tatgagggca cgcgttctga gaattcacac gtgctggctc
      421 tgattaaaga caaaaacgag gttccggaac tacgagcagg tgacatgggc gaggtggtgc
      481 tcgaccaaac ccctttttat gctgaaagcg gcggacaagt cggagatgta ggccgccttt
      541 tcggacacgg cgaggatgaa tcaaccgctg ccgtcgagaa tacatattcc ccggcccagg
      601 gcctgatcgt gcatcaggtc agggttgaga aaggagcctt gaaaatcggc catactgtta
      661 cagccgcggt tgacgcactg aagcgggatg ccacccgaag gaatcatact gctacacacc
      721 tgatgcatgc cgccctgcgt gaggtgcttg ggacacatgt gaaacaggct ggttcggttg
      781 ttgcgcctac ttatttacgg tttgacttta cccattacca gccgctgacg cagacagaga
      841 tcgatgagat cgagaatttg gtgaattatc acattctgcg aaacgagccg gtccaaacga
      901 acgagatggc ggtggaagag gcaatgcgct caggcgcaat ggcgcttttt ggtgaaaaat
      961 acggagagcg agtccgcgtg ctttcgatta acggcgccga agggatcttc tcaaaagagt
     1021 tgtgcggcgg tacacatgtg cgcgcgacag gcgatattgg gttgttcaag atcgtgagcg
     1081 atgaatcgat tgcttcggga gttcgtcgca tacgcgccgt caccggcgtc gatgcgttcg
     1141 aaaggttccg cgagactgaa gccattatcg ataaactcac cggcgatctt cgcacctctc
     1201 gctccgaact gcccggcgtt gtcgttcgat tacaagaaga gctcaagaaa gtgcgtcgtg
     1261 aggcggacga tctgaggctg aagattgcca ccggcgcggc aggtggttct tcgaatggcg
     1321 atgtagcccg cgagattgcg ggagtgcgag tgttggccca agaagcgagc gacctcgacg
     1381 ccgccgggat gcgacagttg tcagacacac tgctcgctcg actcaaatcg ggcgtggttg
     1441 tgctgggccg gagtggcgac ggcaaggtgg cactaattgt tcgcacctca gccgatttga
     1501 cgaagcgagt cccggccgga caggtcatta aagagctggc gccgttggtt ggcggaaagg
     1561 gcggcggcaa gccggacatg gctgagggcg gcggcaccca accagagaac ctccccaaag
     1621 ctttagaagc tagctacggg attgtggagc gcctgctcgg ttaagggcac gtcggtgatc
     1681 gctcgcattt tctgagccct gctccatcgc aacacacagc attggttcgg cataaggcgc
     1741 gaaagtgaat caaaccacaa tcgcgccttt gccctttccg gcaaaccgaa gtgatatagg
     1801 cctcacgatt actactacat catcttctgc ttgtcggtaa gttcaatgcc catcttctcg
     1861 agcgttagct tattcagcgt acctgtgacc ttgatatttt cagcggcttg aaatttcttc
     1921 aagccatccc gcgtggcatc atcaagcttc ccgctttgtt cacctgcgta gaagccgcgg
     1981 ttcttcaaga tgccttgagc ctggttgatc tgatctttgg tggcacgaaa aacaggacca
     2041 cgtctccttg agcttgcgct ttggcctgtg gttgagccgg ttgcagcgct tccggcaact
     2101 ccgagtgaag acacgaaaag aagagacaga gctactgcca gtagtttttt cattttagtt
     2161 tcctcgtttg tgaggcgcgc aggcgcgcgc ggtttttgga aaatgaggtt ccgtaaggcg
     2221 cgcagatgat accgcggcaa cggatcgctt aagcaaggcc aacgcgcaag agcgaaaact
     2281 caatccattg ccaaacgtct tgcatcgcag ctttcgcggc acggggcgag ctagtcttcc
     2341 gtcttgcggg acgag
//
LOCUS       DASUEX010000578         3882 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1602107, whole genome shotgun sequence.
ACCESSION   DASUEX010000578 DASUEX010000000
VERSION     DASUEX010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3882
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1602107"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            156..2747
                     /locus_tag="VFV61_09640"
     CDS             156..2747
                     /locus_tag="VFV61_09640"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX4900784.1"
                     /translation="MNNELSAGTTLSHYRIVSKVGAGGMGEVYLAHDTHLDRTVALKV
                     LLENVAADRARLQRFVQEAKSASALNHPNILTVYEIGHADPVHFIATEFIEGETLRQR
                     IKSGHMKLNDTLNVAMQAADALAAANEVGIIHRDIKPENIMLRKRDGYVKVLDFGLAK
                     LIEQPAVAVNREAPTKSLFKTAEGVVMGTVTYMSPEQAGGLRVDARTDIWSLGVVLYE
                     MVAGCLPFEGSTMSELLASILNQKEPPPLARFTREVPAELERIVEKALRKDREERYQT
                     AKDMLLDLRRLKQKLEVGAELERTVPPESRSAPVEARPSGAQEAVSTPQASSAQTAML
                     EGAPATSSAEYIISEIKQHKRGVVAILAAIILLSVGGIAYGGAAILAAFILLVVAGIA
                     YYFFFARGSSRGAIDSIAVLPLVNASNDPNTEYLSDGISEALINSLTELRQLRVIARS
                     TAFRYKGREVDPRAVGRELNVRSVLMGRVRQIGETLNVQVDLVDASTGAQLWGEEYER
                     KVSDVLAVKQAIAREVTEKLRLRLSGEEQRRLVKRDTTNAEAYQFYLRGRYFWNKRTA
                     EGLKKAIEQFQQAIDRDQNYALGYVGLADCYLVLEEYAGVPANESLVKTRAAADRALQ
                     IDDSLAEAHTSSAYIYQQMWRWAEAEEEFKRAINLNPNYSTAHHWFSLYFRVKRQFDY
                     AMREIKLAQQLDPLSPVISGNVALVYLLKNDPSSAIEQCQRIIELDPSFPEAHSTLGF
                     AYVKRCRYEEATAEFQNAVELSGRVSFYLSYLGYCYAVTGRRAEALRILKELEEKYAR
                     CEAVGVYLAGVHAGLGERDQAFAWLERDFEQRSGQLQYITTRFSLEELRSDPRYADLV
                     LRMGLQP"
     gene            2819..3298
                     /locus_tag="VFV61_09645"
     CDS             2819..3298
                     /locus_tag="VFV61_09645"
                     /inference="COORDINATES: protein motif:HMM:NF024375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,4'-dihydroxyacetophenone dioxygenase family
                     protein"
                     /protein_id="HEX4900785.1"
                     /translation="MTTAFAPMPGIPAALHRAENDLPFVEYQEGVVFQLLHVDVEAGL
                     WVIRVRLQPGVTIQRHKHTGEVFAFTLKGSWHYLEYPEVNTPGSYLFEPAGSVHTLHV
                     PASNSEVTDVWFAIRGANLNLDAEDKVESVIDAGAVLEIYLAQCEASGQGRPNVIGM"
BASE COUNT          904 a         1021 c         1124 g          833 t
ORIGIN      
        1 caacaactcc tggtgatgcc tgaaggtccg atgtctccca tcccgcgcga acacggtatg
       61 tcctgaaaat tttgctgtcc cctaagtaag cagaaagacc ttccgggttt gttacgacga
      121 cacactgatc tagggtccat ggttctctca gaaacatgaa taatgaatta tcagccggca
      181 ctactctctc gcattaccgc atcgtctcca aggtcggcgc cggcggcatg ggtgaagtat
      241 atctggccca tgatacccac ctggaccgca ccgtcgcctt gaaggttttg cttgagaatg
      301 tcgccgctga tcgggcgcga ctacaacgtt ttgttcagga agccaaatct gcatccgcct
      361 tgaaccaccc gaacatcctg accgtctacg agatcggaca cgcggacccc gtgcatttta
      421 tcgccaccga attcatcgag ggcgagactc ttcggcagcg cataaaatcg gggcacatga
      481 agttaaacga cacgcttaac gtcgccatgc aggcagctga cgctctcgca gccgcaaacg
      541 aagtgggcat catccaccgc gacatcaagc cggagaacat catgctccgc aagcgtgatg
      601 gatatgtgaa agtcctggac ttcggcctcg ccaagctcat cgagcagccg gctgtcgcag
      661 ttaataggga ggctccgacc aaatctctgt tcaagacggc agagggtgtc gtgatgggca
      721 ccgtcaccta tatgtcgccg gagcaggccg gagggttaag agttgatgcg cgcaccgaca
      781 tctggtcgtt gggcgtggtg ctgtatgaga tggtcgccgg atgcctgccg ttcgaaggtt
      841 cgacaatgag cgagctactg gcctcgatac taaaccagaa agagcctccg cctttggcgc
      901 ggtttacgcg cgaagtgccg gccgagttgg aacgtattgt cgagaaggca ctgcggaagg
      961 atcgtgaaga acgctatcag accgcgaagg atatgctgct tgacctgcgt aggctgaagc
     1021 aaaaactgga agttggtgcc gagcttgaac gcactgtgcc gcctgagtcg agaagcgccc
     1081 cggttgaagc gagaccttcc ggcgcccagg aagccgtctc aaccccgcag gcttcatctg
     1141 cgcagaccgc aatgctcgaa ggcgcacccg cgacatcgag cgccgaatac atcatcagtg
     1201 agatcaagca gcacaagcgc ggggtggtcg ctattttggc tgctatcatc ctcctctcag
     1261 ttggcggtat cgcttacggc ggcgctgcca ttctggcggc cttcatcctg ctcgtagttg
     1321 caggtatcgc ttactatttt ttcttcgcgc ggggcagcag ccgtggggcc attgattcca
     1381 tcgccgtcct gcctctcgtc aacgcgagca atgatcccaa cacggagtat ctgtcggacg
     1441 gcatctctga ggcgctgatt aacagcctga ccgagttgcg gcagctaagg gtgatcgctc
     1501 gtagtaccgc ctttcgttac aaggggcgcg aagttgaccc gcgggcggta ggtcgtgagt
     1561 tgaatgtgag gtcggtgttg atggggcgcg tgcgtcagat cggagagacg ttgaacgtcc
     1621 aggtggattt ggttgatgcc tcgacgggcg cgcagctttg gggcgaggag tatgaacgca
     1681 aagtctcgga cgtactggcg gttaagcaag cgatcgcgcg ggaggttacg gagaagttga
     1741 ggttgaggtt atccggcgaa gagcaacggc gacttgttaa gcgcgacacg acaaacgcgg
     1801 aagcctacca gttctatttg cgagggcgtt atttctggaa caagcggacg gcggagggac
     1861 taaagaaagc gatagagcag ttccagcagg cgatcgaccg cgaccagaac tacgctcttg
     1921 gatatgtcgg actggctgat tgctatttgg tgctcgagga gtatgccggc gttcctgcaa
     1981 acgagagcct agtgaaaacc agagccgccg ctgatcgcgc cctacagatt gacgactcgc
     2041 tggcagaggc gcacacgtcc tcagcataca tctatcagca gatgtggcgc tgggcagaag
     2101 ctgaagaaga attcaagcgg gccatcaacc tcaatccgaa ttactcgaca gcgcatcact
     2161 ggttttctct ttatttcaga gtaaaacggc aatttgatta tgcgatgaga gagatcaagc
     2221 tggcgcagca gcttgaccct ctttcgccag tcatcagtgg taacgtggcg ctggtatacc
     2281 tcctaaaaaa cgatcccagt tcagcaattg aacagtgcca gaggattatc gaactcgatc
     2341 ccagcttccc ggaagcacac agcactctag gcttcgcgta cgtcaaacgg tgtcggtatg
     2401 aagaggcaac ggcggagttt cagaatgcgg ttgaattatc ggggagagta agtttctact
     2461 tgagttacct gggatactgc tacgccgtta ccggtaggcg agctgaggcg cttcgaatac
     2521 tgaaagaact cgaagagaaa tatgccaggt gcgaagctgt cggagtatat ctagccggag
     2581 tgcatgccgg acttggtgaa agggatcagg cgtttgcctg gctcgaaagg gatttcgagc
     2641 agcgcagcgg ccagttacag tacattacaa ccaggtttag tcttgaagag ctccgcagcg
     2701 acccgcgcta cgccgacctc gtgctccgca tgggactcca gccgtaaaag caaattctgt
     2761 gataaaggtc taacccagga caaccgatta gtgatcacaa ttttcaggag gaatcaaaat
     2821 gactactgcc tttgcgccaa tgcccggcat tcccgcagcg ctgcaccgtg ccgagaacga
     2881 tcttcccttt gtcgagtacc aggagggcgt agttttccaa ttgctgcacg ttgatgtgga
     2941 agccggactc tgggtgatca gagtgcgcct tcaacccggc gtcactatcc aacgccacaa
     3001 acacacgggc gaggtcttcg ccttcacgtt aaagggttcg tggcactacc tggaataccc
     3061 ggaagtgaac acaccaggtt catatctgtt cgagcctgcc ggctcggtcc atacactgca
     3121 tgtgccggcg tcgaactcag aagtgaccga tgtctggttc gccatccgtg gagcgaacct
     3181 caaccttgat gcggaggaca aggtcgagag cgtaatcgac gccggtgccg tgcttgaaat
     3241 ctacctggct cagtgtgaag cgagcggaca aggacgtcca aacgtaatcg gaatgtaagc
     3301 gctgcacact catttgagat gtcgtcgcta ccgagattcc tgagactaat gacaatttgt
     3361 cagcgcacgg gaaaaggcgt ttgaatggtt ggaaaaagac ttccaggcgc gaaacgggag
     3421 actagcggaa atccgctggc aacttcagtt tgattctctc cgtgacgacc cgcgttacga
     3481 ggatttgctt gggcgaatgg gtttgccaca gtagcgtgaa ctggatagtg cttagctgtg
     3541 cacgttaatg aatgggccga aacactgttc gactcgtagt aacgtggaac atgtgtaaat
     3601 aaccggcacc atagtgggac gcagtcagta ccgtaatgcg gtagcgtcgg gttccctttt
     3661 caattgtgaa cttaatggtc aacggaacta cccgacgcta ccgcgttacg gtactgacgg
     3721 cgtcccactc tgaagagcct aatctgtgaa acgctgccct acatgtaatc gcgttgaagc
     3781 cgacgacacg ctcgcttttt gtcgcacaga tggcgtggcg ctcattaacg attctggctc
     3841 ggtgggcagt ggcacggcca gcgcgaagtt tagctctgct cc
//
LOCUS       DASUEX010000579         3665 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1481064, whole genome shotgun sequence.
ACCESSION   DASUEX010000579 DASUEX010000000
VERSION     DASUEX010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3665
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1481064"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..557)
                     /gene="bshB1"
                     /locus_tag="VFV61_09650"
     CDS             complement(<1..557)
                     /gene="bshB1"
                     /locus_tag="VFV61_09650"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB1"
                     /protein_id="HEX4900786.1"
                     /translation="MSPPRPQLDAIAIFSHPDDAELSVAGTLLKLKQQGYRTAVVDMT
                     RGEMGTRGTPEIRAAEADAAARAMKLDLRLNLEQPDGHVWLNDESRTAVVRIIRQYRP
                     KILLTTHWDDPHPDHANTARIVREGARLATMQRYDAESGYPAVKMPALMHSLYSRLVV
                     PSFIVDVSDFVEEKMNAIKTHASQF"
     gene            complement(628..2346)
                     /locus_tag="VFV61_09655"
     CDS             complement(628..2346)
                     /locus_tag="VFV61_09655"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEX4900787.1"
                     /translation="MAELIVRFPDRPPDHFPLGRLRITLGRSARNDLCVPDPFASRVH
                     AEVRREGDEYFLQDLGSANGTLYNGLTVQGSVALTAGGRIQIGETEIVFDDGTYNSSM
                     GATMITDNSSSIPEATIALASGDRTTSGLLEAIEGARTKPEEIVATRAGKQGDLLALI
                     SKVGITLLASASLNETLDQIVSLVFEAVPADRCLLMMREDSGQELRVAVARLRDRVGE
                     VGEIRVSRNVLDEVVMRGKSVLTSDAQHDPRFASGTVVLQGIRSVLAVPLGVADKVFG
                     IIYADSPIAEGRFTEDHLKLLTTLASVAAIRVENARLLEAQLQQERLERELQLAMEIQ
                     QRFQPTAPPIVLGYELQGISFPCYEIGGDYYDFIERDDGRLVIALGDVSGKGTAAALL
                     MSSLHAAVHAQAGSHETLVETISAVNRYLADNIPPNRFVTLFYAELDPASGALSFLNA
                     GHNPPLIVHAAGTVEQLASGGLPLGIKRDAEYREGRTQLQLGDVLVIYSDGVTEAASP
                     SGEEFGPTRLYEVVSRNIDASAAGLRDRIESALTKFSQGTQAADDITLVIVKRQSETK
                     QMAAVR"
     gene            complement(2390..2887)
                     /locus_tag="VFV61_09660"
     CDS             complement(2390..2887)
                     /locus_tag="VFV61_09660"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEX4900788.1"
                     /translation="MTARVERAAIKRPEVIKRPRRDLVERRPAPFSLRCGAALIDYIL
                     LVGIVAFSTIIARWFGGGARTAGNSAETVGVLISFVVALVNYVLLAGLTGRTIGKWAT
                     GLRIEQKDGDVPGIGIALFRHFVGYPLSCLTLGLGFLMAIFSTRGRALHDLIAGTVVV
                     RDSAF"
     gene            complement(2929..>3665)
                     /locus_tag="VFV61_09665"
     CDS             complement(2929..>3665)
                     /locus_tag="VFV61_09665"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX4900789.1"
                     /translation="GATLFHLLTGSDPQDNPLLIFDFTKNPRPRQIVPSISSEFEQIL
                     MRAVEYKPEDRFPSAGDLRDALATHLENLMAGRVSYGMPATQLGHETVQVQTVYCGFC
                     GGRIAADDVFCAHCGAKQPLAGVGASSTLRAPRPTAKLVVAGTTELDASFILKKDNNL
                     VGRADPHSNIFPEIDLSRFDVETKVSRRHARIWLEGETFLVEDLGSVNGTVINDSVRL
                     APRQPRVLDSGDKLRLGETTLHFLLG"
BASE COUNT          803 a         1058 c         1048 g          756 t
ORIGIN      
        1 tagaactgcg acgcatgcgt cttgatggcg ttcatctttt cttcgacgaa gtcggaaaca
       61 tcaacaatga acgacggcac taccaatctc gaatacaggg agtgcatgag cgccggcatc
      121 ttcactgccg gatagccgct ctcagcgtcg tagcgctgca ttgtcgccag ccgtgccccc
      181 tcgcgcacga tgcgggcggt gttcgcatga tcgggatgcg gatcgtccca atgcgttgtc
      241 agcaagatct tcgggcggta ctgacgaatg atgcgcacga ccgcggtgcg cgactcgtca
      301 ttcagccata catgaccatc gggttgctcc aaattcaaac gcaaatctaa cttcatcgcc
      361 ctggctgcgg catccgcttc cgcggcgcga atctccggcg tgccgcgcgt ccccatttca
      421 ccgcgagtca tgtccactac cgctgtgcga tatccctgct gtttaagttt gaggagagtg
      481 ccggcgacag acaattctgc atcatccgga tgagagaaga tcgcaatggc atcgagctga
      541 ggtcttggag ggctcatcgt gttcgtaggt ttggctgaac cggctattaa ttgccgttct
      601 gcaaacagtc agagctcccg catcggttta gcggactgcg gccatttgct ttgtttcgga
      661 ttgtcgtttg acgatcacga gcgtgatgtc atctgcggcc tgggttccct gcgagaattt
      721 tgtcagcgcc gattcaatgc ggtcgcgcag acccgcggcc gaggcatcaa tgttccgcga
      781 aacaacctcg tagagacgcg ttgggccaaa ctcttcgcct gaaggactcg cagcttcggt
      841 tacgccgtcg gagtaaatta ccagcacatc gcccagttgc aattgcgtgc gcccctcccg
      901 atattcggca tcccgcttaa ttcccaacgg tagtccgccg gaagccaact gttctacggt
      961 cccggcagca tgaacaataa gtgggggatt atgacctgcg ttaagaaacg aaagcgcgcc
     1021 cgacgccgga tccagttcgg cgtagaagag tgtgacaaag cgatttggcg gtatgttgtc
     1081 cgccagatag cgattcaccg ctgagatggt ttccacaaga gtctcatgcg aacccgcctg
     1141 ggcatggacc gcggcatgaa gcgaggacat caagagcgcg gcggcagttc ctttgcccga
     1201 aacatcgccc agcgcaatta ccagacgccc atcgtcccgt tcgatgaagt cgtagtagtc
     1261 gccgccgatt tcataacagg gaaagctgat gccctgtagt tcgtaaccca ggacgatagg
     1321 tggagcagtt ggttgaaagc gctgctgaat ctccatcgct aactgcagct cgcgctccag
     1381 gcgttcttgt tgcagttgcg cttcgagcag gcgcgcgttt tcgacgcgaa tcgcggcaac
     1441 tgacgcgaga gtcgtcagca gcttcaagtg atcctcggta aaacgtcctt cggcgatcgg
     1501 cgaatcggca tagatgatac cgaatacctt gtccgccacc cccaacggca cggccagcac
     1561 cgaacggatc ccctgcaaga ctacggtgcc gctggcgaag cgcggatcgt gttgcgcgtc
     1621 ggaagtgagc acggacttac cgcgcatgac cacttcatca agcacattgc ggctgacgcg
     1681 gatttcgcct acctcgccca cgcgatcgcg cagtcgcgcc acggccactc gtaattcctg
     1741 tccgctatct tcgcgcatca tcagcaagca acggtcagcg ggcactgctt caaacacaag
     1801 cgacacgatt tgatccaagg tctcattgag tgaggccgag gccagcagcg tgatgcctac
     1861 cttgctgatg agggccagca gatcgccttg tttaccggcg cgcgtggcca caatctcttc
     1921 cggcttcgtg cgcgctcctt cgatagcttc gagcagtcct gacgtagtgc gatcgcctga
     1981 ggcgagcgcg atggtggcct cgggaatcga gctgctgtta tcggtaatca tcgtggcacc
     2041 catactcgag ttgtaggtgc catcgtcaaa aacgatttct gtttcgccga tctggatgcg
     2101 cccgcctgcg gttagcgcca ccgagccctg cacggtaagg ccgttgtaaa gggtgccgtt
     2161 agccgagcct aaatcctgaa ggaaatactc gtcgccctcg cgacgcactt ccgcgtgaac
     2221 tcgcgacgca aatggatccg ggacgcacag atcattacgc gcggagcgtc cgagggtgat
     2281 gcgcaaacgg ccgagcggaa aatggtcggg aggccggtcg gggaatctaa cgatcagttc
     2341 agccattttt tgaaacgatc cctaagggct atgggaaaac tacggtttac taaaaagcac
     2401 tgtcacgtac tactacagtg ccggcaatca aatcatgaag cgcacggcca cgggtactaa
     2461 agatggccat cagaaatccg agccccagtg ttaagcatga caaagggtac ccgacgaagt
     2521 gtcggaaaag cgcgatgccg atgccgggaa cgtcgccgtc cttttgttcg atgcgtaggc
     2581 cagttgccca cttaccgatc gttcgcccgg tcaagccggc gagcaagaca tagttgacca
     2641 gggctactac gaacgaaatt agcacaccga cagtttcggc agaatttcct gcggtgcgag
     2701 cgccgccgcc aaaccaacgg gctatgatcg tgctaaaggc gacgattcca acgagcaaaa
     2761 tatagtcgat caaggcggcg ccacagcgca gagaaaacgg tgcgggtcgt cgctccacca
     2821 agtccctgcg agggcgtttt atcacctcag gccgcttaat cgcggctcgt tcaactcgtg
     2881 cagtcattaa gatgttcagt ggtctttatt ggcggttggc aacggcggtc atcctaaaag
     2941 aaaatgaagc gtcgtttcgc cgaggcgaag tttgtctccg ctgtccaaaa cccgaggctg
     3001 gcgaggcgcc agcctcaccg aatcattgat taccgttcca ttcactgagc cgagatcctc
     3061 gaccaggaaa gtttcgcctt ccaaccaaat ccgcgcatga cgccgcgaga ccttggtctc
     3121 gacatcaaag cgagagagat cgatttccgg aaaaatgttt gagtgcggat cagcgcgtcc
     3181 aaccaggttg ttgtctttct tcagaatgaa gctggcgtcg agttcggtgg tgccggccac
     3241 caccaatttc gccgtcggcc gcggtgctcg cagggtcgag ctagcgccga cgccggctaa
     3301 tggttgcttc gcgccacaat gggcgcaaaa gacatcgtcg gcagcgatgc ggccgccaca
     3361 aaagccgcaa tagaccgttt gcacctgcac ggtctcgtgg ccaagttgcg tcgccggcat
     3421 tccgtagctc acgcggccag ccatcaaatt ctcaagatga gttgcgaggg cgtcgcgcag
     3481 atcgcccgcc gaggggaagc gatcctcagg tttgtattcc acagcgcgca tcaggatctg
     3541 ttcaaactcg ctcgagattg agggaacgat ctggcgcggc cgcgggttct tagtgaagtc
     3601 aaagatcagc aggggattat cctgaggatc agaacccgtt agcagatgga acagggtggc
     3661 gccga
//
LOCUS       DASUEX010000580         6115 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1354526, whole genome shotgun sequence.
ACCESSION   DASUEX010000580 DASUEX010000000
VERSION     DASUEX010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6115
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1354526"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="VFV61_09670"
     CDS             <1..226
                     /locus_tag="VFV61_09670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900790.1"
                     /translation="TEIDETVASLSVLANRPESLPQVPGASGGIGIDLEGGYGGVMLG
                     RPNPDGTWETKCVFTFAEGAEFLGLEESTE"
     gene            242..1924
                     /locus_tag="VFV61_09675"
     CDS             242..1924
                     /locus_tag="VFV61_09675"
                     /inference="COORDINATES: protein motif:HMM:NF014301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA domain-containing protein"
                     /protein_id="HEX4900791.1"
                     /translation="MKNKTSLMKLFVTFVALVFIGLSAGNTFAGEAQFVIVNINAAGV
                     GFNDPTPAAPVGGNTGTTLGEQRLIAFQHAAAIWSARLDSNVPIRIRAQFTPLGAGVL
                     GSAGPRDVFRDFPNAPLAGTWYHVALANKLAGVDLNPTNDDINANFSTNFNFYLGLDN
                     NHGALNDLVAVLLHEFAHGLGFSQNASLTTGALLSGFPDAYNTRLFDLTQGLTWPQMT
                     NAQRVTSATNFGRVVWTGAFVTAGVPNVLSFGSPEVRVLNPVAIAGIYQFGTAGFGPA
                     IGNPNVQADVVAAVDAVEAGGTSTDGCSAFSNAAAVAGKIALIERGLCGFAVKARNAT
                     DAGAAAVIIYNQAANVNAAPPGMADDGINGQFVTIPTVSVRRADGLAIAGGGTVTARI
                     GVDPSIRAGADALGRARVYAPFPVVGGSSISHYDTVARRNLLMEPAINADLTHKVKAP
                     DDLTFELLRDVGWTFPDADNDGVPNDEDCNPNSNLSPTIIIGGIDTGVPNTLFANGCT
                     MADILAQLAADHTNNHGDYVSAVAALTNQWVADGLITGRQKGAIQSAAAQLP"
     gene            complement(2044..5325)
                     /locus_tag="VFV61_09680"
     CDS             complement(2044..5325)
                     /locus_tag="VFV61_09680"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX4900792.1"
                     /translation="MNPEQWRKISELFQAALDRPVDERAGFLANACAGDDEIQQRVEA
                     MLAADARNDLMMDGPAYEILSAPSPSLLGPDASQSFSGEMIGAYQLTRELGRGGMGTV
                     YLAYDTRLQRQAAVKLLPSRLSNNPERVYRFQREARSASALNHPNIITIYDFGKENGR
                     DYIASEFVEGLTLRHFVGSEDLTLNKILDVVIQVASALEAAHAAGIVHRDIKPENIML
                     RPDGYAKVLDFGLAKLTEPESDDNKPKRKADTAGIRSDFATRAGILIGTANYMSPEQV
                     RGQKVDGRSDLFSLGVVLYELITGHQPFGGETWHHTMVAITDYDPPPLARSVQGIPPA
                     LQEILSRVLAKSCEQRYQTARAFLTDLETLRSELAATAHIERIGAERAKAIESGEQTA
                     TVTSDGESIDEPAKDKLESAIGLPAYFKRRNLIAAIAIIILGTVAAYFYLGSGFTNKR
                     ALTDKDTILLADFDNTTGDVDFDGPLKQGLRVQLAQSPYLNIFPEERARETLGLMERT
                     HDQKITRDVGREICQRRGIKVLLVGSIASLGRNYVITLEAINSQSGEAIAQQQTEAEG
                     KEQVLKALGRAATSMREKLGESLASIRKFDAPIEQATTASLEAFKDYTVGVELWRKGQ
                     YAQSVPPLKRAIERDSEFALANAQLGTSYRDLRNLALGNKYLERAYQLRDRVSERERL
                     EITATYFRHITGELDKRIETTSLLTRTYPQDPGSFHLHGNSLMIAGEFEQAAEAYREA
                     LRLDVDFALSRANLALALIGLNRFDEAQAVIEQGIARHLDSSGLHNRLYLIGFFKGDA
                     EAMKRQVEWFAGRPEEYQIRELQARSLAFAGRRREASETLAQAAQLAGGRGLVAEKAR
                     ILANEANMNAIFGFTQPARNQALRVLTLIEKEHIAPEELLPSLIGQLDSQPLVCTLAL
                     CGEARRAQSLADELGRRFSEDTMHNSVWLPMVNATLDLQRGSAAGTDKAIELLQPARQ
                     YEAAFSFRPTWLRGQAYLQAKNGVLAAAEFQRIIEHRGWDVLSPLWPLAHLGLARAAI
                     MQGDIPKARKAYEDFFLLWKQADVELPVLIEARKEYEATANLSSQKWQHDRMAFPSTF
                     RNLVN"
     gene            complement(5343..5900)
                     /locus_tag="VFV61_09685"
     CDS             complement(5343..5900)
                     /locus_tag="VFV61_09685"
                     /inference="COORDINATES: protein motif:HMM:TIGR02999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX4900793.1"
                     /translation="MTSPASPNVTEMLIAWGRGDEAARDALIPLVYDQLRRIARHHLR
                     GERAGHTLQTSELINEAYLKLVEQSVSWQSRAHFFGISARLMRQVLVDYARAKQRLKR
                     GGDQQQISLTDFEDAPKQVADLLALNTALETLAEVDQQKSRIVELRFFGGLTIEETAE
                     VMGISTPTVERGWRAARAWLQTELG"
BASE COUNT         1271 a         1758 c         1674 g         1412 t
ORIGIN      
        1 gacagagatt gacgaaactg tcgcgagcct gagcgtgctc gccaaccggc ccgagagctt
       61 accgcaagtt ccaggcgcca gcggcggcat cggcatcgat ctggagggag gctatggcgg
      121 agtgatgctc ggccgtccca accccgatgg tacttgggag acaaagtgcg tcttcacgtt
      181 cgccgaaggc gctgagttcc tgggtttgga agaatccact gaataggagg tcgatgcaca
      241 gatgaaaaac aaaacatcgc ttatgaagct ctttgtaaca ttcgtggcat tagtgttcat
      301 cggcctttcg gccggaaaca cctttgcagg tgaggctcaa ttcgtaatcg tcaacattaa
      361 cgcggcgggt gtgggattca atgatccaac cccggcagca cccgtaggtg gtaataccgg
      421 aacaacgctc ggcgagcaac gtctgatcgc cttccagcat gcggcagcga tttggagtgc
      481 gcggctggat agtaatgtcc caatccggat tagggcacag ttcacaccac tgggcgcggg
      541 tgtactcggc agtgcaggtc cgagagacgt atttcgtgac tttcctaacg cgcctctagc
      601 cggcacctgg tatcacgttg cgctcgcaaa taaacttgcg ggagtggacc tgaaccccac
      661 taatgatgac atcaacgcta atttcagcac caacttcaat ttctatttgg gtctcgacaa
      721 taatcacggt gccctgaacg atctggtggc ggtgttgcta cacgagttcg cgcacggact
      781 cgggtttagc caaaacgcca gcctaaccac aggcgcactc ctgagtggtt tccctgatgc
      841 gtacaacacg aggctctttg acctgactca gggactaacc tggccacaaa tgacaaatgc
      901 ccaacgtgtt acttcggcaa ccaattttgg cagggtcgtg tggactggtg cctttgtgac
      961 cgccggtgtt ccgaacgtgc tttccttcgg aagtcctgaa gtgcgcgtgt tgaatccagt
     1021 ggctatcgcc ggaatctacc agtttggtac cgctggcttt ggaccggcga ttgggaatcc
     1081 gaacgttcag gctgacgttg tagcagcagt agatgcggtc gaggcaggag gaacttcaac
     1141 tgacggttgc tctgcttttt caaacgcggc agccgtggcg ggaaagatcg ccctaattga
     1201 acgcgggctc tgtggatttg ccgtcaaggc tagaaatgca accgatgctg gggcggcggc
     1261 agtgataatt tacaatcaag ctgccaacgt gaatgcggca cctccgggta tggcagacga
     1321 tggaatcaat ggtcagtttg tgactatccc caccgttagc gttcggcggg ctgatggtct
     1381 ggcaatcgct ggtggtggca ccgtcaccgc gagaatcggt gttgacccat ccatccgcgc
     1441 cggggctgac gcactcggca gggctcgcgt ctatgcgccg tttccagttg tgggcggctc
     1501 ttcaatctca cactacgaca ctgttgcgcg ccgcaacttg ctcatggagc cggcgattaa
     1561 tgcggatttg actcacaagg tcaaggctcc ggatgatctg acatttgaac tattaagaga
     1621 tgtcggttgg actttccccg atgccgataa cgacggcgtt cccaatgatg aggactgcaa
     1681 tcccaattcg aatttgagtc cgacgatcat tattggcggc atcgacactg gtgtacccaa
     1741 cacgctcttc gctaacggat gcaccatggc cgacattttg gctcagctcg ccgctgacca
     1801 cactaacaac cacggcgact acgtttcggc ggttgccgcg ttgactaacc agtgggttgc
     1861 agatggtttg attactggaa ggcagaaggg cgcgattcaa agcgctgccg ctcagcttcc
     1921 gtagttaggc cggctaatca gcgctgttca gccagcgcgg gatagctagc acagcaagtg
     1981 ccagccaaaa caaaagggtt gacggcaacg tcaacccttt ttccgtttag cctgatcaaa
     2041 aactcaatta accaaattac gaaaggtcga tggaaatgcc attcgatcgt gttgccactt
     2101 ttgagaactc aagtttgccg tggcctcgta ttctttcctt gcctcgatca aaacgggcag
     2161 ctcaacatcc gcctgcttcc acagcaggaa aaagtcttca taggctttgc gggccttcgg
     2221 aatatcgccc tgcattattg cggcccgcgc cagtccgagg tgcgccagcg gccagagcgg
     2281 tgaaagcacg tcccagccgc gatgttcaat gattctttga aactccgccg ccgcaagcac
     2341 tccgttcttc gcctgcaaat acgcctgtcc gcgcaaccag gtcggcctga acgagaaggc
     2401 ggcttcgtac tgtcgcgccg gttgcagcag ttcaatagct ttgtccgtac ctgccgccga
     2461 accgcgctgt agatccaaag tcgcattgac cattggcagc caaactgaat tgtgcatcgt
     2521 gtcttctgaa aacctgcgcc cgagttcgtc agcaagcgac tgggctcgtc tcgcctcacc
     2581 acataatgcc agcgtgcaga cgagtggctg tgaatccaat tgcccgatga gtgaaggcaa
     2641 caactcctcg ggtgcgatgt gctccttttc aatcaaagtc agaacgcgca acgcttgatt
     2701 ccgggccggt tgcgtgaacc caaaaatcgc attcatattg gcttcattag ccaggattct
     2761 ggccttctcc gccaccaagc ctcgccctcc cgccagttgg gccgcttgcg ctaacgtttc
     2821 gctggcctct cgccgccggc ctgcaaaggc caacgaccta gcctgaagtt cacggatttg
     2881 atactcctcc ggccttcctg cgaaccactc tacctgacgc ttcatcgcct cggcgtcgcc
     2941 cttaaagaat ccaatcaagt agagccgatt gtgcaagcca ctggaatcca ggtgtcgcgc
     3001 aatcccttgt tcgatgaccg cttgagcctc gtcgaaccgg ttcaatccga ttaaagccag
     3061 cgcgaggttc gcgcgagaga gggcgaagtc aacatccagg cgcagcgctt cccggtaagc
     3121 ctcggccgct tgttcaaact ctcccgcgat cattagcgaa ttcccatgga gatggaatga
     3181 gcctgggtct tgcggatagg ttcgtgtcaa cagcgaagta gtttcaatgc gcttatcgag
     3241 ttcgccggta atatgcctga aataggtcgc agtgatttcc agccgctctc gctcgctcac
     3301 ccggtcgcgc agttgatagg cccgctccag atacttgttg cccagtgcca gattgcggag
     3361 atcgcggtac gaagtcccga gctgagcgtt ggccaaagca aattcgctgt cgcgctcaat
     3421 ggcacgtttg agtggtggga ctgactgcgc atattgcccc ttccgccaga gctcaactcc
     3481 gacggtgtaa tccttaaagg cctcgagcga ggcggtcgtt gcctgttcga ttggcgcatc
     3541 gaacttacgg atcgaggcga gcgattcgcc cagtttctcg cgcatagatg tcgcagcccg
     3601 tccgagcgcc ttgagcacct gctctttgcc ttcggcttcg gtttgctgtt gtgcaatcgc
     3661 ttccccgctc tggctgttga tcgcttccag cgtgatcacg tagttgcgcc cgagactggc
     3721 aatggacccg accaggagca ccttgattcc ccgccgctga cagatctcgc gtcccacgtc
     3781 gcgggtgatc ttctggtcgt gtgtccgctc catcagcccc agtgtctctc tggcgcgctc
     3841 ttcggggaag atgttgagat acggcgactg tgcaagctga acccgcagcc cttgtttcag
     3901 cggaccgtca aaatccacat cgcccgtggt gttgtcaaag tcggcaagca gaatagtgtc
     3961 tttgtcggtc agtgcccgtt tgttagtgaa tccactacct aagtaaaaat atgcggcgac
     4021 tgtccccaga atgatgatcg ctatggcggc gatgagattg cgccgtttga aatacgcggg
     4081 caacccgatc gcgctttcca acttgtcctt tgcgggttca tcaattgact ccccatcgga
     4141 tgtcactgtt gccgtctgct caccactctc aattgccttt gcgcgttctg cgccgatccg
     4201 ctcaatgtgt gccgtcgccg cgagttcgga tcgcagtgtt tcgagatcgg tcaggaaagc
     4261 ccgcgcagtt tgatagcgct gctcacagct cttagccaac acgcggctaa ggatctcctg
     4321 caaagcggga ggtatgcctt gcacagaacg cgcgagtggc ggcggatcgt aatcagtaat
     4381 cgccaccatt gtgtggtgcc acgtctcacc accgaatggt tgatgcccgg tgatcagctc
     4441 gtaaagaacc actcccaggc taaacagatc gctacgtccg tcaactttct gaccgcgcac
     4501 ctgttctggc gacatgtaat tggcggtacc gatcaggatt ccggcccgcg tcgcaaaatc
     4561 agaccgaata cccgccgtgt cagcttttct cttaggtttg ttgtcgtcag attctggctc
     4621 ggtcaatttt gccaacccga agtcgagcac tttggcgtaa ccgtcgggac gcagcatgat
     4681 attttctggc ttgatgtcgc ggtggacgat gccggcggcg tgcgcggctt cgagcgcact
     4741 ggctacctga atcaccacgt cgagaatttt attgagtgtt agatcttcgc tgccgacgaa
     4801 atggcgcagt gtaagaccct ccacaaattc cgaggctatg tagtcgcgtc cattctcctt
     4861 accaaagtca tagatggtga tgatgttggg atggtttagg gcactagctg agcgggcctc
     4921 tctttggaaa cggtagacac gctccggatt gttgcttaac cgcgaaggca ggagttttac
     4981 cgccgcctgg cgttggagcc gcgtgtcgta ggccagataa accgtcccca ttccaccgcg
     5041 ccccagttct cttgttagct ggtaggcgcc gatcatttcc ccgctgaaac tctgggaagc
     5101 atcaggccca agaagtgaag gcgaaggtgc gctaaggatt tcgtaggctg ggccatccat
     5161 catcaggtcg ttccgcgcat cggcagctaa catggcttcc acgcgctgct gtatctcgtc
     5221 atcgccggcg caggcgtttg ccaggaagcc ggcacgctcg tcaaccggac gatccagagc
     5281 agcctgaaaa agctcgctaa tctttcgcca ctgttcggga ttcatctttt ggacttttag
     5341 cgtcagccca actcggtttg cagccaggcg cgggcagctc gccatccgcg ttcgacggtg
     5401 ggagttgaaa tgcccatgac ctcggctgtc tcttcaatcg tcaatccgcc gaagaaacgt
     5461 aactcaacga tcctgctctt ttgttgatca acctctgcca gagtttcgag cgctgtattg
     5521 agtgcaagta gatcggcgac ttgtttcggc gcatcttcga aatcggtaag cgaaatctgc
     5581 tgctggtcgc cgccgcgctt cagcctttgc tttgcgcgcg cgtaatccac cagcacctgg
     5641 cgcatcagcc gagccgagat accaaagaag tgtgcccggc tctgccacga aaccgactgc
     5701 tccaccaact tcaaataagc ttcattaatc aactcagagg tttgcagtgt gtgaccagca
     5761 cgttcaccac gtagatgatg gcgcgcaata cgacgcagct gatcatagac gagcgggatc
     5821 aacgcgtctc gtgccgcttc atcgccccgc ccccacgcaa tcagcatttc cgtcacgttt
     5881 ggcgatgcag gactggtcat agtgctccct caagtgggcc ctaacgcgac cattagagca
     5941 gaggatgcgc gtgatcaaaa gcgcctttgc cctgaaagtc gttagctata ccgaaacggc
     6001 tttgccgcct gatgtgcggc ggtggcttcg ccccgccgtt aggtgcagga ttcgattggg
     6061 ctatcccctt ggttcggctg cgcccttact cgtgtcgaga ttgatagcat tcatt
//
LOCUS       DASUEX010000581         3063 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_189562, whole genome shotgun sequence.
ACCESSION   DASUEX010000581 DASUEX010000000
VERSION     DASUEX010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3063
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189562"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            140..1216
                     /gene="pstS"
                     /locus_tag="VFV61_09690"
     CDS             140..1216
                     /gene="pstS"
                     /locus_tag="VFV61_09690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEX4900794.1"
                     /translation="MPLSTRITSSLIGILSLSLLTLALACSGRAPEAGGNVQLQGAGA
                     TFPYPLYQKWMSEYGKLHPNIQIDYQSIGSGGGIKQIQAQTVDFGASDSPMKDADLKA
                     APGELLHVPTVLGAVVLTYNLSSISQPLRFSSDVIADIFLGKLKRWNDPRIQADNPGV
                     NLPAADVVVVHRSDGSGTSAVFTDYLSKVSSEWKSKVGTGTSPSWPIGIGGKGNEGVT
                     GQVKNTPNTIGYVELAYAGQNNLPVALIKNRAGEFVAPSLDAVTAAAAESISSTPDDL
                     RVSITDPAGKATYPISSYTYILVYKDQKNEAKGKALVDFLWWGIHDGEQHAKPLQYAP
                     LPAEIVKRAEAKISSITSGGKPLR"
     gene            1244..2308
                     /gene="pstC"
                     /locus_tag="VFV61_09695"
     CDS             1244..2308
                     /gene="pstC"
                     /locus_tag="VFV61_09695"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEX4900795.1"
                     /translation="MRGANSQHGEPLNAQVALRIEQVGRTEAADSPTAGPKRATPNFW
                     RRTFSPTGNRGDAVFRIAMFATALLMVLIISAMIVALARNSLLSIRQFGFHFLLNQTW
                     DPIKGEFGALSFIYGTVVSSFLALLICVPLSLGIAIFLVEQAPQVVSRPIAFLVELLA
                     AIPSVVFGLWGIFVLAPFLRVHVEPVLAQWFGWLPLFRGPITGIGLLTGGLILAIMVT
                     PIISAVVRDVLAAVPSSQREAALALGATKWETTRVVLTNAAPGIAGAIILGLGRAVGE
                     TMAVTMVIGNRPRISASLFDPSYTIASAIANEFTEATQDLYLSALVELGLILFLVTFV
                     VNAIARVLVWNVTRKAGTVS"
     gene            2311..>3063
                     /gene="pstA"
                     /locus_tag="VFV61_09700"
     CDS             2311..>3063
                     /gene="pstA"
                     /locus_tag="VFV61_09700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEX4900796.1"
                     /translation="MFTSSGKAGRARRLKNAVMTSLSALCTIVAVSILLVILIYIARQ
                     GLGSLNLQFLLDRPRPVGEGGGIGNAILGSVVLLLLSSIIGIPVGIATGIYLSEFGND
                     HFGSLVRFLVDTLTGIPSIVTGVFVYTILVLPQKHFSALAGGVALALIMVPIVARTTE
                     EMVRLVPQNLREGALALGAPQWRVTLGIVLPAAASGIATGAMLAIARISGETAPLLFT
                     AFGSRFFSYYLDQPIASLTVQIYSYAISPYDEW"
BASE COUNT          626 a          839 c          846 g          752 t
ORIGIN      
        1 ctgggcctga atatccaggg tgtccaaccc gcaccaatgt tcacagagag ttcaggccca
       61 gttcagatgt ctgtaacatc acccacctag agttgtaatc gttcaaattt agacttttct
      121 accttatcag gaggatcgaa tgcccctctc aacccgaatc accagctccc taatcgggat
      181 tctttcattg agtttgttga cgctggccct ggcttgttcc ggccgggccc ccgaagccgg
      241 aggaaatgtc cagttgcagg gtgcgggtgc aacgtttccg tatccgctct atcagaagtg
      301 gatgagtgag tatggcaagc tccatcccaa tatccagatt gattaccagt cgatcggatc
      361 cggaggtggg atcaaacaga tccaggcgca gaccgtcgat ttcggcgcct ctgattcgcc
      421 catgaaagac gctgacctga aggccgcgcc gggtgaacta ctgcatgtac cgaccgtctt
      481 gggagctgta gtgctcactt acaatctgag tagtatttcg caaccactcc gtttctcttc
      541 agacgtaatc gccgacatct ttctgggcaa gttaaagaga tggaatgacc cgcgaattca
      601 agccgacaat cctggtgtca acctgccggc cgcggatgtt gtcgtggtcc accgctcaga
      661 tggcagcgga acgtctgcag tgttcactga ttatctatcg aaggtgagtt cggagtggaa
      721 gtcgaaagtc ggcaccggta cctcacccag ttggcccatt ggaatcggcg gtaaaggaaa
      781 tgaaggtgtg acgggccagg tgaagaacac cccaaacacg attggctatg tggaactcgc
      841 ctatgccggg cagaacaacc tgccagtcgc tctgataaaa aacagagccg gcgagttcgt
      901 tgcgccctcg ctggatgcag tcacagccgc agccgcagaa tccatcagca gtactcccga
      961 tgacctgcgc gtttcgatca ccgatccggc aggcaaggct acctatccga tctccagtta
     1021 cacctacatc ctcgtctaca aggatcagaa gaacgaggcc aagggcaaag cgctggtgga
     1081 tttcctctgg tgggggattc acgacggaga acagcacgcc aaaccccttc agtacgcgcc
     1141 acttccagcc gagatcgtca agcgtgcgga agccaaaatc agctcgatta catccggagg
     1201 caagcccctt cgctaatcgc gagcgcacgg agatggagct cagatgagag gcgcgaactc
     1261 acagcacggc gaaccattga acgctcaggt tgcgttacgg atcgagcaag ttgggaggac
     1321 tgaagcggct gattctccaa ctgctgggcc aaaacgtgcc accccaaact tttggcggcg
     1381 cacgttttca cccaccggca acaggggtga tgcggtcttt cgcattgcga tgtttgcgac
     1441 ggcattactg atggtgctca tcatcagcgc catgatcgtt gctctcgcgc gtaactcgtt
     1501 gctctcaatt cgccaattcg gctttcattt tctacttaat cagacctggg atccgatcaa
     1561 gggcgaattc ggcgcgctct cattcattta cggaacagta gtctcttcgt ttctggcgct
     1621 gctaatctgc gtaccgcttt cgctgggtat tgcgatcttt ttggtcgagc aagcgccgca
     1681 ggtggtgtct cggcccatcg cctttctggt tgaactattg gcagcaattc cctcggtagt
     1741 ctttggcctc tggggaatct tcgtcctcgc tccgtttctt cgtgttcacg ttgagccagt
     1801 tctggcccag tggtttggct ggctaccctt gtttcgtggc ccgatcaccg gcattggtct
     1861 gctaactggc ggactgatac tggccataat ggtgacccca atcatctcgg cagtggtgcg
     1921 ggatgttctt gccgctgtgc ccagctctca acgtgaagct gcgctcgcat taggcgccac
     1981 gaagtgggaa accacccgcg tagttctgac gaatgctgcc ccgggaattg cgggagcgat
     2041 cattctcggg ttgggtcgcg cggtgggcga aacgatggcc gttacgatgg tcatcgggaa
     2101 tcggccgagg atcagcgcct cgcttttcga cccctcgtac accattgcct ctgccattgc
     2161 caatgagttt accgaagcca ctcaggatct ttatctaagc gcattggttg agctgggatt
     2221 gattctcttc ctggtaactt ttgttgtgaa cgccatcgcc cgcgtgctgg tttggaatgt
     2281 cacgcgcaag gctggaacgg ttagctgaaa atgttcactt cgagcggcaa agcaggtcgc
     2341 gcacggcgac taaaaaacgc ggttatgacg tcgttatcgg cgctgtgcac gattgtggcc
     2401 gtcagtatct tgttggtgat actgatttac attgcgagac agggtctcgg ctctttgaat
     2461 cttcagtttt tgctggaccg gccgcggccg gtgggagaag gcggcggcat tggaaacgcg
     2521 atcctcggtt cggtggtgtt gttactgctg tcgtcaatca tcggaatacc ggttggcata
     2581 gccacaggta tctatctttc cgagttcggc aacgatcact ttggcagtct ggtgcgtttt
     2641 ctcgtggaca cgctaacggg cataccttca atcgtcactg gtgtctttgt ctatacgatt
     2701 ctggtattgc cgcagaagca cttctcagcc ttggcagggg gcgttgcact cgctctcatc
     2761 atggttccca ttgtcgctcg cacaaccgaa gagatggtca ggctggtacc tcaaaatttg
     2821 cgcgaaggcg cacttgcatt aggcgcgcca cagtggcgcg tgactttggg gattgttctt
     2881 cccgctgcag cttcggggat cgcgactgga gcgatgctcg ccatcgcgcg tatttcaggt
     2941 gaaacagcac cgcttctttt cacggcgttc gggagccggt tttttagtta ctaccttgat
     3001 caaccgatcg cgtctttgac cgttcaaatc tacagctacg ccatctcgcc ttatgacgag
     3061 tgg
//
LOCUS       DASUEX010000582         2248 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1602815, whole genome shotgun sequence.
ACCESSION   DASUEX010000582 DASUEX010000000
VERSION     DASUEX010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1602815"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..815)
                     /gene="preA"
                     /locus_tag="VFV61_09705"
     CDS             complement(<1..815)
                     /gene="preA"
                     /locus_tag="VFV61_09705"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF006183.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HEX4900797.1"
                     /translation="MDLSIVVNGMKFPNPFLLGSGPPGTNARVIAKSFDAGWGGAVAK
                     TVSLESAKVVNVVPRYGKLRSKVSGEVIGFENIELISDRPIDVWLEEFRQLKKDYPHH
                     ILIASIMEEYEKGRWQELTKLVQDTGVDALELNFSCPHGMTERKMGSEMGEHPDLTEE
                     VTGWVTEVAKIPVWAKMTPNITNIKEPSHAAVRGGAQGISAINTILSVIGVDLETLRP
                     MPTVEGHTVPGGYSSQAVRPIALRMVSQLALSLPKEVSISGIGGIESSHDAIE"
     gene            complement(857..1264)
                     /locus_tag="VFV61_09710"
     CDS             complement(857..1264)
                     /locus_tag="VFV61_09710"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEX4900798.1"
                     /translation="MSTIERFEDLEAWKRARSFAKLIHAVSGTGDFGRDFGLRDQIRR
                     AANSIVSNIAEGFERDGDKEFLQYLFVAKGSCGEVRAQLYLAIDYEYITEQEFSQLKM
                     KALELNRIISGLIKYLKQSHFTGKKYKARSYKT"
     gene            complement(1316..2185)
                     /locus_tag="VFV61_09715"
     CDS             complement(1316..2185)
                     /locus_tag="VFV61_09715"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEX4900799.1"
                     /translation="MPRTVKCGLIQCANAAPTDLPVDDIKRLNIEKHLGFIEKAAAQG
                     VQMLCMQEVFTTPYFCAEQPTRWYDAVEKIPDGETVKLMQEVAKTYGMVLIVPIYEEE
                     MTGIYYNTAAVIDADGKYLGKYRKNHIPHVNPGFWEKFYFRPGNLGYPAFDTAFARIG
                     VYICYDRHFPEGARALGLNGAEIIFNPSATVAGLSEYLWKLEQPAHAVANGYFVGAIN
                     RVGHEQPWDIGEFYGQSYFCDPRGRILVEAPRDQDALIVADLDLEMIREVRNTWQFFR
                     DRRPDTYGALVAD"
BASE COUNT          501 a          603 c          580 g          564 t
ORIGIN      
        1 aactcgattg catcgtgcga agattcaatg ccgccgatgc cgctgattga gacttctttg
       61 ggcaggctga gcgcgagttg actaaccatc cgcagggcga tgggccggac tgcttgagaa
      121 gaataaccac cgggcaccgt atggccttcg actgttggca tcggccgcag cgtttccaaa
      181 tcgacgccaa tcaccgagag gatggtgttg atagcggaga ttccctgggc gccgccgcgc
      241 acggcggcat gtgatggttc ctttatgttg gtgatgttgg gcgtcatctt tgcccaaacc
      301 ggaatcttgg caacctctgt tacccagccc gtgacctctt cagttagatc aggatgttca
      361 cccatctctg aacccatctt tcgttcggtc atgccgtgtg gacaggagaa gttaagttcc
      421 agagcatcca cacccgtgtc ttgcacaagt ttggtcaatt cctgccagcg acccttttcg
      481 tactcttcca tgatcgaggc gattaggatg tggtgaggat agtctttctt tagctgacga
      541 aactcctcga gccaaacatc gatggggcga tcggagatga gttcgatgtt ttcaaagccg
      601 attacctcgc cggagacttt tgagcgcagc tttccatagc gcggcaccac attgaccact
      661 ttggctgact cgaggctgac ggtcttagcc accgcaccgc cccaaccggc gtcgaaagat
      721 ttggcaatca cgcgagcgtt cgtgcccgga ggtccggagc cgagcagaaa cgggttggga
      781 aatttcattc cgttaacgac tatcgataga tccatttttt cactccgttc aagtctcagg
      841 tctcaggtct caagtctcaa gtcttgtagc tcctggcctt gtacttcttg cccgtaaagt
      901 gcgactgttt taggtactta atcaaacccg aaataatgcg gtttagctcc agagccttca
      961 tcttcagttg tgaaaactcc tgctcggtta tgtattcata atctatcgca agatacaact
     1021 gcgctcgcac ctcgccacac gaaccctttg ctacaaaaag atactgaaga aactccttgt
     1081 cgccatctcg ttcaaaacct tcagctatgt tagagactat ggagttcgcc gcccgacgaa
     1141 tttgatccct gagtccgaag tcgcgaccga aatccccagt accactgacg gcgtgtatca
     1201 gctttgcaaa gcttcgtgca cgcttccaag cttctagatc ttcgaatctt tcaatcgtac
     1261 tcatagagtc ctcggtgagg tttggacttg agacctgaga cctgagaccc gataactagt
     1321 ccgcgaccag cgctccgtaa gtatccggcc ggcgatcgcg gaagaactgc cacgtgttgc
     1381 gcacctcgcg gatcatttcg agatcaaggt cagcaacaat caaggcatcc tgatcacgcg
     1441 gtgcttccac aaggatgcga ccgcgcggat cgcaaaaata actctgcccg taaaactccc
     1501 caatgtccca tggttgttca tgcccaacac gattgatggc gccgacgaag tatccgttgg
     1561 cgacggcgtg agcaggttgt tccagcttcc acaagtattc agaaagtccg gcgactgtcg
     1621 ctgaagggtt gaaaatgatt tcggctccgt tcaaaccgag cgctcgtgcg ccctcgggga
     1681 aatggcggtc ataacagatg tagacgccga tgcgagcgaa tgccgtgtcg aaagctggat
     1741 agccaaggtt accgggccgg aaataaaact tttcccagaa gccggggttc acatggggga
     1801 tgtgattctt gcggtatttg ccgaggtact taccgtccgc atcaattacc gccgccgtgt
     1861 tgtaataaat gcccgtcatc tcttcttcgt agatgggaac aatgagtacc attccgtaag
     1921 tcttcgctac ttcctgcatc agttttacgg tttcaccgtc gggaattttc tcgacggcgt
     1981 cgtaccaacg cgtcggctgt tcggcgcaga agtaaggtgt cgtgaagact tcctgcatgc
     2041 aaagcatttg cacaccctgc gcggccgcct tttcaatgaa gcccaggtgc ttttcaatat
     2101 tgagacgctt gatgtcgtcg acgggcaagt cagtaggtgc ggcgttggcg cattggatta
     2161 gtccgcattt aactgtgcgt ggcatgatgt ctccagttca actccagatt caggttcagt
     2221 gtcgttcagt ttcggtaaga gagtgttt
//
LOCUS       DASUEX010000583         2813 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1240125, whole genome shotgun sequence.
ACCESSION   DASUEX010000583 DASUEX010000000
VERSION     DASUEX010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2813
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1240125"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(110..2674)
                     /locus_tag="VFV61_09720"
     CDS             complement(110..2674)
                     /locus_tag="VFV61_09720"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HEX4900800.1"
                     /translation="MKLAKLLSMIIALVAFGSASQSQTNLESRRIAKIKIPVHTGWQF
                     REAGKDTWYAATVPGSVHTDLLNNKLIEDPFYRDNEQRLQWIGKTDWEYQTTFYVTPE
                     ILARNNIELVFEGLDTYANVFLNDSSLLNADNMFRTWRTDCKRLTRPGANTLRIRFRS
                     PINEVLPLMAKMDYQLPAGNDQGEKTSPHTRKAPYQYGWDWGPRFVTSGVWRPVFLEA
                     WDRARVENLHIVPKQVTPEEASLAAAVEVVADNDTTATIVVDNLRDKTLAARRQVNLK
                     RGSNRVSLDFVIKDPALWWPNGLGAHPLYTFKARLLLDGKRIDETSARTGLRSLELRQ
                     QPDKSGKSFTFVINGVPVFAKGGNWIPADSFPTRISKAKYRELLESVRDTNMNMLRVW
                     GGGIYERNDFYELCDEMGILVWQDFMFGCSLYPGDDAFLENVRQEAIDNVKRLRNHPS
                     IVIWNGNNEIESGWFHWGWKDQLPAKLWDDYRKLFYGVLPEVCNALDPSRPYWPSSPS
                     SNLEDDNESQKMGDLHYWQVWHASLPFSEYEKQFPRFMSEYGFQSFPQIETVNTYTVP
                     ADHDIKSPVMMAHQRHPRGNQLIREYMLREYPEPKDFESFLYVSQVLQAEGIKIGAEH
                     LRRIMPHNMGSLYWQINDCWPVASWSSIDYTGRWKALQYYARRFYSEILVSPQEENGN
                     INVFVVSDQVQPAAAQLNLSLLDFDGNKLWGQQQDIEVAPLKSKSYLTIPIDKLMAAQ
                     DPKRVLLFTEVVVGGKRVSSNEHFFKPYKELAVPRPQIKTDIMPVRGGFKVTVSADKF
                     ARAVYLSAPGSVGSFTDNYFDLVPGEKMEVEFRRGPGITLNDFRKQLKVRTMADAF"
BASE COUNT          624 a          768 c          741 g          680 t
ORIGIN      
        1 aacactagat cacccgacga gccagcaaag ctggcggaga gagtgatagc ccccggacaa
       61 gtagccgctt cttcacttca tctctcggtt tgcgattctg tacaagcact taaaaagcat
      121 cggccatggt gcgaaccttg agctgtttgc gaaagtcgtt cagggtgatc ccagggccgc
      181 ggcgaaattc gacttccatt ttctcgcccg gaactaaatc gaagtagtta tccgtaaagg
      241 aaccgacgga acctggcgct gataagtaca ccgcccgcgc aaacttgtcg gcagacacgg
      301 tgaccttgaa tccgccgcgc acgggcatta tgtctgtctt gatttgcggc cggggcacgg
      361 caagctcctt gtacggttta aaaaagtgtt cattgctcga cactcgctta ccgcccacta
      421 caacctcggt aaacagaagc actcttttgg gatcttgcgc tgccatcagc ttgtcgatcg
      481 ggatcgtcaa ataagatttg cttttgagcg gagcaacctc aatgtcctgc tgttggcccc
      541 acaacttgtt gccatcgaag tcgagcaggc tcaggttcaa ttgcgcagcc gcgggctgta
      601 cctgatcaga gaccacgaag acgttgatat tgccgttctc ctcctgcggg ctaacgagaa
      661 tctcgctgta gaagcggcgc gcgtaatact gcagcgcctt ccatcgaccg gtgtagtcga
      721 tactggacca cgaggcgacc ggccaacagt cgttgatctg ccagtagagt gagcccatgt
      781 tgtgcggcat gatgcgtcgc agatgctcgg ccccgatttt tatcccttcg gcttgcaaca
      841 cctggctgac ataaagaaac gactcgaaat ccttcggctc cggatactcg cgcagcatgt
      901 attcacgtat tagctggttg ccacgaggat gccgctggtg ggccatcatc actggggatt
      961 tgatgtcatg gtccgcaggc acagtgtagg tgttgactgt ctcgatctgc ggaaacgact
     1021 gaaagccgta ttcgctcatg aaccgcggaa actgtttctc atactcgctg aacggcagcg
     1081 aggcgtgcca aacctgccaa tagtgaagat cacccatctt ctgggattca ttgtcatctt
     1141 caagattgga actgggcgaa cttggccaat aaggccgcga tggatccagc gcgttgcaaa
     1201 cttccgggag cactccatag aagagcttgc ggtaatcatc ccagagctta gccggcagct
     1261 ggtccttcca gccccagtga aaccagccag actcgatttc attgttgccg ttccaaatca
     1321 ctatgctcgg atgattgcgc aagcgtttta cgttatcgat tgcttcctgc cgcacgttct
     1381 caagaaatgc gtcgtcgccg ggatacaagc tgcagccgaa catgaaatcc tgccagacga
     1441 gaattcccat ttcgtcgcag agttcataga aatcgttgcg ttcgtaaatg cctccgcccc
     1501 agacccgcag catgttcatg tttgtgtcgc gcaccgattc caacaactcc cggtatttgg
     1561 ctttgctgat gcgcgtggga aaactgtcgg cgggaatcca gttgccgcct ttagcgaaaa
     1621 cgggaacccc attgatcaca aaagtgaagc tcttgccgga cttgtcaggc tgctgtcgga
     1681 gttccaatga ccgcagaccc gtacgtgctg atgtttcatc gatccgcttt ccatccagca
     1741 gcagccttgc cttgaacgta tagagtggat gagcgccgag cccgttgggc caccagagcg
     1801 cagggtcctt aataacgaag tcgagtgaca ctcgattgga gccacgcttt aggttcacct
     1861 gccgtctcgc cgccaaggtt ttgtccctga ggttatcaac aacgatggtg gcagttgtgt
     1921 cgttgtcggc aacaacttca acagcggctg ccaggcttgc ttcttcagga gttacttgct
     1981 ttggcacgat gtgcagattt tccacgcgcg ccctatccca agcctccaaa aagaccgggc
     2041 gccaaacacc gctggtgacg aaacgcgggc cccagtccca cccgtattga tatggagcct
     2101 tgcgcgtgtg cggacttgtt ttttcgccct gatcgttgcc ggcgggcagc tggtaatcca
     2161 tcttcgccat aagcggcaac acttcgttga tgggcgaacg aaagcgaatc ctcaacgtat
     2221 tggcgccggg tcttgtcagc cgtttacagt cggtccgcca ggtgcgaaac atgttgtcgg
     2281 catttagcag ggatgagtca tttagaaaga cgttggcgta ggtatcgagg ccctcgaaga
     2341 ccagctcaat attgttgcgg gcgagaattt cgggagtgac gtagaaggtg gtttgatact
     2401 cccagtcagt tttgccgatc cattgcagcc gctgttcgtt gtcacgatag aagggatcct
     2461 caatgagctt gttgttcagc agatcggtgt gcacactgcc cggcacggtt gccgcgtacc
     2521 aagtgtcttt gccggcctcg cgaaactgcc agccagtgtg gacaggaatc ttgatctttg
     2581 cgattctccg cgattcaagg ttggtttgcg attgagaggc actgccgaag gctactaaag
     2641 caataatcat ggacaagagt ttagcgagct tcattcttct tttatcttaa ttatgtaaac
     2701 tcagcgatgc cataaagacc tgtcctgccc cggctaattc gtgaagaaat tccgtgttaa
     2761 gatgcgctcc attcctttgc ctaacgccac tccaacgagt ttatcccgct tca
//
LOCUS       DASUEX010000584         2895 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_101169, whole genome shotgun sequence.
ACCESSION   DASUEX010000584 DASUEX010000000
VERSION     DASUEX010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2895
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101169"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            250..1869
                     /locus_tag="VFV61_09725"
     CDS             250..1869
                     /locus_tag="VFV61_09725"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX4900801.1"
                     /translation="MMIFQDGPIVTPEQLFEQYFSESVYKTWGNISPLYRLDSFDWTI
                     LILYFTILTILAIYGGYRVKQVIDFWRYRKFIPQPKARFAEADLPFITVQLPLFNEMY
                     VVERLVKAITEIDYPREKFEIQVLDDSTDETVKLGEATVQKYADLGFDISYIHRSDRT
                     GFKAGALEAGLKVAKGELLAIFDADFVPKPDCLRKLVDYFTDPMVGCAQMRWSHINGT
                     YNLLTRLQTIMLDGHFVVEQTTRNRTGGFFNFNGTAGIWRRRAIAMSGGWQHDTLTED
                     TDLSFRAQLMGWKFVYLLDEDAPAEIPVEINAFKAQQRRWAKGVMQVGIKLYPRIWRA
                     RLPLRVKLEMFFRLTGNISYPLMIVASFLQFPLLLVRYNQPFYHLMILDLPLLFFSSI
                     SVFLFYGTAVWYLDEKRAPRLAHLPLVMALGIGLAFSNAKAVLEALLGVKSEFVRTPK
                     YSVEKTTDETWKAKKYKRKRGFLPLLELGFALYFALAILYAGRLHMWGTIPFLSLFFL
                     GFGYMGVMSIMQTTSGRRLLSLWRPVAERRT"
     gene            2039..>2895
                     /locus_tag="VFV61_09730"
     CDS             2039..>2895
                     /locus_tag="VFV61_09730"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEX4900802.1"
                     /translation="MISTPLTRIGLIVICLLSFSTAFYAQSQATTGNIEGRVVDAGGA
                     VLPGVTITARNPSTGFVKTTTSDEEGNYRLIVLPPGRYTVTASGAQGFADASFENVQV
                     TVGGQTALSIQLGVAGIATMVDVEAEGQIIETTRSSVATTVNQRAIENLPVNGRNFLD
                     FATLTPGVVRDPTRAGDLAVGGQKGTLNSLQVDGADNNNTFFGQSFGRTGTRPPYQFS
                     EESVQEFQVNQNGFSAEFGRAGGAVINVVTKSGTNQWHGSAFEYFRDESLNSNTPIFT
                     ARGAKRPKS"
BASE COUNT          645 a          792 c          757 g          701 t
ORIGIN      
        1 gcagagcgaa tcgcctccgc tccgagcgat tacagcgccc cgctccgccg gatctgagtt
       61 aataacttcc aagcaacccc gatcatcggg attgccgcca ctctccaaaa tctcccgctt
      121 tcgttcgcat ccattcctcc gaaagcagca tcaaaacgcg tccagaaatg cgggtttctg
      181 ttaacgagcg ccttgggtta gaatccggtg cctgaatttt tgtagagcgg tagatcgccc
      241 agactcttaa tgatgatttt tcaagacggc ccgatagtca cgcctgaaca gctcttcgag
      301 cagtatttca gcgagtcggt ctataagacc tggggaaata tcagccccct ctatcgtctc
      361 gactctttcg actggaccat cctgatcctc tatttcacga tccttactat cctggcgatc
      421 tatggcggct atcgcgtcaa acaggtgatc gacttttggc gttatcgcaa attcatcccg
      481 caaccaaaag cgagatttgc ggaagccgat ctgccgttta tcacagtgca actgccgctc
      541 ttcaacgaga tgtacgtggt cgaacgactg gtgaaagcaa tcaccgagat cgactatcct
      601 cgggaaaaat ttgagattca ggtacttgat gattcgacgg acgaaacagt aaagctggga
      661 gaggcgacgg ttcagaagta cgccgatctg ggattcgaca tcagttacat ccaccgcagc
      721 gatcgcacgg gtttcaaggc tggagcgctt gaagccggat tgaaagtggc gaaaggcgaa
      781 ctgctcgcca tcttcgacgc cgactttgtt ccgaaaccgg attgcttacg gaagctggtt
      841 gactacttta ctgacccgat ggttggttgc gcccagatgc gctggtctca cattaacggc
      901 acttacaacc tgctcacccg attgcaaacg ataatgctcg acggccactt tgtggtcgag
      961 cagacgacgc gcaaccgcac tggtgggttt ttcaacttca acggcacggc cggaatctgg
     1021 cgccgtcgtg ccatcgccat gagcggcggc tggcaacacg atacgttgac cgaagatacg
     1081 gatcttagtt ttcgtgcgca gttgatgggt tggaagtttg tttacctgct cgacgaagat
     1141 gcccccgcag agattccggt tgagattaac gccttcaagg cacaacagcg acgctgggcc
     1201 aagggcgtga tgcaggtggg tatcaagctt tacccacgga tttggcgggc gcgtttgccc
     1261 ctgcgtgtga aactggaaat gttctttcgc ctcacgggaa acatcagtta cccgctcatg
     1321 atcgtcgcca gcttcctcca gtttccgttg ttgctcgttc gttacaacca gccgttttac
     1381 cacttgatga ttctcgacct gccgctgctg ttcttttcat cgatctcagt cttcttgttc
     1441 tatggcacag cagtctggta cctggatgag aaacgtgcgc cgcggctcgc gcacctgccg
     1501 ctcgtgatgg cgctgggcat tggtttggcg ttttcgaatg cgaaggcggt gctggaagca
     1561 ctgctgggag tgaagtcaga atttgttcgc acgccgaagt acagcgtcga gaaaactact
     1621 gatgagacct ggaaggcgaa gaagtacaag cgcaagcgcg gttttctgcc gctgctcgaa
     1681 ttaggctttg cactttattt tgcgctcgcc attctttacg ccggacgctt gcacatgtgg
     1741 ggcacgattc cgttcctctc gctattcttc cttggctttg gctacatggg cgtcatgagc
     1801 attatgcaaa cgaccagtgg ccgacgactg ctctcacttt ggcgcccagt agccgagcgc
     1861 cgcacctaag agcgcgaacc gcctaagttt tatctttaat agttctcttg ctttgatttc
     1921 ggacgtgaaa tagtatcctc gcgttcgaat actatttgga accactcttt ttgaatcgaa
     1981 ggcctgaatc tcaccggcac ttcgtcaagt tgacccagaa aactcaagga gaatcacaat
     2041 gatctcgacg cccctaacga gaatcggtct gattgttatt tgcctcctgt cattctcgac
     2101 cgctttctac gcccaatccc aggccacgac tggcaacatc gagggtcgcg tagttgatgc
     2161 cggtggtgcc gtccttcccg gagtgacaat caccgctcgg aatccgagta ctggttttgt
     2221 caaaaccacc acctccgacg aggaaggaaa ttaccgcctt atcgttctac ccccgggcag
     2281 atacaccgtt acagcgagtg gagcccaggg cttcgccgat gctagctttg aaaatgtcca
     2341 ggtcacagtc ggcggacaga ctgcgctttc tattcagtta ggcgttgccg gtatcgcaac
     2401 catggtggat gtggaggccg agggtcagat catcgaaacc actcgtagct cggttgctac
     2461 gaccgtaaat cagcgcgcca tagaaaacct tccggttaat ggccgcaact ttttggactt
     2521 tgccactctg actcccggtg tggtgcgcga tccaactcgt gcaggagact tggcggtcgg
     2581 cggtcaaaag ggaacgctga acagtctgca ggttgacggg gccgataaca acaatacgtt
     2641 cttcggccaa tcctttggcc gcaccggaac acgcccgcct taccagttct cggaagaatc
     2701 ggtgcaggag ttccaggtga atcaaaatgg attctcggcc gagttcggac gagcaggcgg
     2761 cgcagttatt aacgtagtta ccaaatcagg cacgaaccag tggcatggaa gtgcgtttga
     2821 atacttccgt gatgagtctt taaactccaa cacccctatt tttacggcgc gtggagcaaa
     2881 gcgacctaag tcgca
//
LOCUS       DASUEX010000585         4575 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_101940, whole genome shotgun sequence.
ACCESSION   DASUEX010000585 DASUEX010000000
VERSION     DASUEX010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4575
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101940"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="VFV61_09735"
     CDS             <1..190
                     /locus_tag="VFV61_09735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900803.1"
                     /translation="SLLDDLRSTKRARRFATAWSGATVRLAGAFRKRGEKPAAAEVPA
                     EAQSEPDAERIRASVSGD"
     gene            complement(337..1848)
                     /locus_tag="VFV61_09740"
     CDS             complement(337..1848)
                     /locus_tag="VFV61_09740"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX4900804.1"
                     /translation="MRRTIISLLLVLSVFTPAAAFNRSQKAMTTKTASVLLPSRAPLI
                     SFRILFMTGAASDPKGKEGVAALTAAMLSQGGSRAMTYEQIVEAMYPIATSFGSQIDK
                     EMTVFQGATHIDNLDKYYGLISQMLLDPGFRQDDFNRLKADSINYLKVSLRESNDEEL
                     GKEYLYNLIYAGHPYEHNNMGTVSSLEKLTLADVQAFYKAHYTQANMVLGLVGGYPKN
                     FPDKVKADFAKLPAGTAANANYDDPKLTPGMHIAIVQRETRATAVSLGFPIAVTRADK
                     DWPALALVASYFGQHRSSNSYLYQRLRESRGLNYGDYAYIEYFPRGMFQFMPDPNQGR
                     SSQIFQIWIRPVVPENGHFVLRAALYEYDKLVREGMSKETFEATREFLIKNNNILTQT
                     QDAQLGYALDSRYYNIPDYVSYMREQLTKLTLEDVNNAIRKYLKSDAMRIVMITKDAE
                     GLREAIVANKPSPITYNSPKPKEITDEDKVIETYKINVKPEDVVIVPVSKVFQ"
     gene            complement(1848..3233)
                     /locus_tag="VFV61_09745"
     CDS             complement(1848..3233)
                     /locus_tag="VFV61_09745"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX4900805.1"
                     /translation="MRLIIILSLLICVPALTLARSGAPQKPRKLFPYNYTIDDLPNGL
                     RLVTVPTDYPNLVALYIVVATGSRNEVEPGKSGFAHFFEHMMFRGSENYTAEQREAIF
                     KRAGAETNAYTSDDRTVYHAVFAKDDLDEIMKMEADRFQRLKYALPEFQTESLAVKGE
                     YDKNSANPFSKLYEVLRETAFKTHTYSHTTMGYLKDIQDMPNQFEYSQEFFKRFYRPE
                     YTTVVLVGDVTQQRALDLTKKVFGDWKRGDYEAKIPAEPAQTEPRTAHIDWPSPTLPI
                     VAVAFRGPAYSDDKKDKLALDLLAQVAFGENSELYQKLVLKEQKVDVLGPDFSNQIDP
                     ELFVVLARVKDPKDVDYVRDQIQTTFKRFTTELIPAAKLDATRSRTRYSFAMAMNSSA
                     AIASTLAPFISLRRTPETIDKLAALMDTITSQDIRDLAARYFRDESRTIVTLATKAKE
                     TANNSGQEDEE"
     gene            complement(3521..3922)
                     /locus_tag="VFV61_09750"
     CDS             complement(3521..3922)
                     /locus_tag="VFV61_09750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900806.1"
                     /translation="MLTIEFSRRLAYIGGVITPLAETIRRWSTWQQTPANFFDDYLLG
                     ALLIYGAWRTGRNVHDGQRFLIAAWAVACGLGYYSFFGQLQSWSRNEADPAPISSLWI
                     AVIKGIAWAIAILALIVSLQRLPNTDAEVDN"
     gene            complement(3928..4314)
                     /locus_tag="VFV61_09755"
     CDS             complement(3928..4314)
                     /locus_tag="VFV61_09755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900807.1"
                     /translation="MTSVLGYARTFFVGGHYRPAPLPSLAEEVQRFHEMLVDLGALLE
                     GDSPLLEEMTAERLLQGPFADAMTHAGQLALLRRLSGNPVAPENFIVANIDPRRLGGQ
                     QPDPVSPDEVWPEAPTGWHRTTKTST"
BASE COUNT         1016 a         1199 c         1304 g         1056 t
ORIGIN      
        1 ttctctgctc gatgatctgc gttctacaaa acgcgcgcgc cgttttgcga cggcctggtc
       61 gggcgcaaca gtgcgtttgg cgggagcatt caggaagcgc ggcgaaaaac cggcggctgc
      121 cgaagtaccg gcggaagctc aatcagagcc ggacgctgaa aggattcggg caagcgtcag
      181 cggcgactaa accaaatgat gaatgcggaa tgatgaatgc ggaattagtg gaatgagggt
      241 acgaaggcag aatgatgaac gcgtaatata caacttccca atgaaccacc ggagtcaccc
      301 ttttattccg cattcatcat tccgcattca tcattcctac tggaacacct tactgaccgg
      361 cacaatcacc acatcctcag gcttcacatt gatcttgtaa gtttcgatca ctttatcttc
      421 atccgtgatt tctttcggct ttggcgaatt gtaggtgatc ggtgatggct tgtttgccac
      481 aatggcctcg cgcaatccct cagcgtcttt ggtgatcatc acaatccgca tcgcatcaga
      541 cttcagatac ttgcgtatgg cattgttcac gtcttcgagc gtcagcttgg tgagctgttc
      601 gcgcatgtaa ctgacgtagt cggggatgtt gtaataacgg ctgtcgagcg cgtagccgag
      661 ttgcgcgtcc tgagtttgcg tcagaatgtt gttgttctta atcagaaact cccgcgtcgc
      721 ctcaaaagtc tctttcgaca ttccttcgcg caccagtttg tcatactcgt aaagcgcagc
      781 ccgcaaaaca aaatggccgt tttcgggaac gactgggcgg atccaaatct ggaaaatctg
      841 cgatgaccga ccctgattgg ggtcgggcat gaactggaac atgccgcgtg ggaaatactc
      901 gatataggca tagtcgccgt agttgagccc gcgggattcg cgtagccgct gatagagata
      961 gctgttgctg gatcggtgtt ggccgaagta tgaagcgact agcgcaagcg ccggccaatc
     1021 tttatcagcg cgcgtcaccg caatcggaaa gccaagcgag actgcagtcg cgcgtgtctc
     1081 acgttgaacg atcgcgatgt gcattccggg agtaagtttc ggatcgtcgt aattcgcatt
     1141 ggcagcggtt cccgccggta gcttcgcaaa gtccgccttc accttgtcag gaaaattctt
     1201 gggataacca cccaccagac ctaacaccat gttggcctgc gtgtagtgtg ccttatagaa
     1261 agcctgcaca tcggccaggg tcagcttctc cagcgagctt actgttccca tgttgttgtg
     1321 ttcgtaagga tgcccagcgt aaatgaggtt atagaggtat tccttgccga gctcctcgtc
     1381 gttgctctcc cgcagcgaca ctttcaaata gttaatggag tcggctttca gccggttgaa
     1441 atcgtcctgc cgaaatccgg gatccaacag catctgacta atcaaaccgt aatacttgtc
     1501 gagattgtcg atgtgggtgg cgccctggaa gacggtcatt tccttgtcga tctgcgaccc
     1561 gaatgaagta gcaatgggat acatcgcttc cacgatctgt tcataagtca tcgcccgcga
     1621 gccgccctgc gacaacatcg cggcagtcag cgccgccacc ccctccttgc ccttgggatc
     1681 ggaagcagcg ccagtcatga agagaatgcg gaacgagatg agcggcgcgc gactcggcaa
     1741 gagcacactg gcagttttcg ttgtcatagc cttttgtgag cggttaaaag cggccgccgg
     1801 agtgaagacg gaaagaacga gcaataggct gatgattgtg cgacgcatta ttcttcgtcc
     1861 tcctgcccgg agttatttgc ggtctctttc gctttggtgg cgagcgtgac gatggtgcgg
     1921 ctctcatcgc ggaaatacct cgcggcgagg tcgcggatgt cctgggacgt aatggtgtcc
     1981 atcaaagccg ccagtttgtc gatcgtttcg ggcgtgcgac gtagcgagat gaatggcgca
     2041 agcgtcgagg caatcgccgc cgagctattc atcgccatgg caaagctgta gcgcgtacgc
     2101 gaacgagtgg cgtcgagttt tgccgcggga ataagttcag tggtaaagcg cttgaacgtc
     2161 gtctgaatct gatctcgcac gtaatcgacg tccttcgggt ctttcactcg tgccagcacc
     2221 acgaacagct ccggatcgat ctgattactg aagtcaggtc cgaggacatc aaccttttgt
     2281 tctttgagca ccagcttttg gtaaagctcg gaattttccc caaaggcaac ctgtgccaaa
     2341 aggtcgaggg ctagtttgtc tttcttgtca tcggagtagg cgggaccacg aaatgccacc
     2401 gcaacgatcg gcaaagtcgg ggagggccaa tcaatgtgag ctgtgcgcgg ttccgtctgg
     2461 gctggttctg cagggatctt ggcttcgtag tcaccgcgct tccaatctcc aaaaaccttt
     2521 ttcgttaaat cgagcgcgcg ctgctgggta acatccccaa ccagtactac ggtggtgtac
     2581 tccggacggt aaaagcgctt gaaaaactcc tgactgtatt cgaactggtt cggcatgtcc
     2641 tgaatgtctt tcaggtagcc catcgtggta tgcgagtaag tgtgcgtttt gaaagcagtc
     2701 tcgcgcaaca cttcatagag tttagaaaat ggattggcgc tgttcttatc gtattcaccc
     2761 tttaccgcca acgactcggt ctgaaactcg ggcagggcat acttcaaacg ctgaaaccga
     2821 tcggcttcca ttttcatgat ttcgtccaaa tcatctttgg caaaaaccgc gtggtagacg
     2881 gtgcgatcgt cggaggtgta ggcattggtt tcggcgcccg ctcgcttgaa gatcgcttcg
     2941 cgctgctccg cggtgtagtt ctcgctgccg cgaaacatca tgtgctcgaa aaagtgagcg
     3001 aagccactct tgccaggctc aacttcgttg cgcgaaccgg tggccacgac gatatagagc
     3061 gcgaccaggt tcggataatc cgtcggcacg gtcaccagtc gcaacccgtt cggcaaatcg
     3121 tcgatagtgt agttgtaggg aaacagcttg cgcggcttct gcggtgctcc gctcctggcc
     3181 agggtcagag ccggcacaca gatcagcaaa gacaagatga ttattaatct cattctaaag
     3241 tactccgtga atcgcgaaat gaagtgggga acgggggcag tatagcgtag tttggcggaa
     3301 ggagggacgc gagcagcgct cggcatgaaa cggcttgccg ccagatgtga ggcgcggctt
     3361 tgccgcgccg tatcaagcac tccgattgtg gggctaggcc ccgagtgtca ctcgttttct
     3421 tgttggggag cagcggaatc ctgggcgcag cccaataaag ttaagtgtcc gctacggcgc
     3481 ggcagagcca ctcctcacat cgggcggcaa agccggttac ctagttatcg acttcagcgt
     3541 ctgtattagg taagcgctgt aagctcacga tcaaagcaag aattgcaatg gcccacgcaa
     3601 tgcccttgat taccgctatc cagagagaag aaatgggcgc gggatcggct tcgtttcgac
     3661 tccagctctg caactggcca aagaaggagt agtagccaag tccgcaggcg actgcccagg
     3721 cggcaatgag aaaacgctga ccatcatgaa cgttgcgacc agtccgccac gctccgtaaa
     3781 tcagcagcgc tcccaacagg tagtcgtcga agaaatttgc cggtgtctgc tgccaggtgc
     3841 tccacctgcg gatcgtttcg gcgaggggag taataacgcc gccgatgtac gcgagacggc
     3901 gactgaattc aatcgtgagc atgatctcta agtagaggtc ttggtggtgc ggtgccagcc
     3961 cgtgggtgct tcgggccaaa cctcatcggg gctgacagga tcaggttgct gccctcccag
     4021 gcgccgcgga tcgatgttgg ccacgatgaa gttctccgga gccacagggt ttcctgacag
     4081 tcgtcgcaac aaagccagtt ggcctgcgtg tgtcatagcg tcagcgaacg gaccttgaag
     4141 caagcgttcg gctgtcattt cttcaagcaa tggcgagtcg ccttccagta gtgcgcccag
     4201 gtccacgagc atttcatgga aacgctgtac ttcttcggcc agggaaggga gtggcgccgg
     4261 ccggtaatgt ccgccgacga aaaaggtgcg cgcatatccg agcacgctcg tcatgtgaca
     4321 aacgagttca gacggagtcc gggtctctga tcctgcgcgg aacgatccaa aatcagaggg
     4381 tgcatctcgg agcgcttttt gagtgcgata cgcgagcgct gcgaggaagt gtcgtaacag
     4441 atttcgttgt tcggctgagg ctgccatcgc gcgaaactta actgctgaga cgcgaagagg
     4501 ctgagtcgca aagaaaacgt ccaataaatt ccgtcatttg tgatgaaacc ctggctgttt
     4561 tcttagcggc tctgt
//
LOCUS       DASUEX010000586         3519 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1691578, whole genome shotgun sequence.
ACCESSION   DASUEX010000586 DASUEX010000000
VERSION     DASUEX010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3519
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1691578"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            334..408
                     /locus_tag="VFV61_09760"
     tRNA            334..408
                     /locus_tag="VFV61_09760"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:366..368,aa:Val,seq:cac)
     gene            681..3107
                     /locus_tag="VFV61_09765"
     CDS             681..3107
                     /locus_tag="VFV61_09765"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX4900808.1"
                     /translation="MIPESIAHYRIIKKLGAGGMGEVYLALDTKLDRKVAIKILHPDQ
                     LAEEHLKKRLVREAQAAAKLDHPNICAIYDVNEANSFTFIVMQYIEGELLAEKMERQP
                     LTLSTTLAMVEQAAEGLAEAHAQGIVHRDIKPQNMIVTPRGQLKILDFGLAKQLHQSG
                     TVDNEAATATLLSKPGLVVGTMPYMSPEQVKGEPLDASSDIFSLGITLYEMLAGKHPF
                     KDKSAAVTMSRILLGEPIPTEKFQTQVSPELQALLGKMLRKDKAERYQNAQDFLTDLR
                     QLPVPPPGHETQANAGRTQEASAIRQPEKAGDSFLSKARRNKWPLLASVLALVLIAVG
                     ISTWLSKEHSDSLAILPFTYTSSDPQLMANPDREYLSDGMTESIINNLSQLTNLKVIA
                     RSSVFRYKGKDMDVQAIGRELNVRTVLVGKIIQEGEGLRIVVDLMDVQRNSQIWGERY
                     ERKTSDIQSVQREIATRVSEKLGLKLTGADQSQLAKSYTNSGEAYESYLKGRYHWNKR
                     TDEGFKQAIQFFREAIDKDPTYALAYTGLADCYTLQSDYGFIPAQEGYALAKGAVTLA
                     LKYDDSLAEAHTSLASIKAATDWDWQGAENEYRRAIELNPKYATAHHWYGAQLFLQGR
                     FDEALPQLRTAQQLDPLSLGINKDLAVALLYARDYEKALEQCRKTLEIEPSFHVMSTY
                     MAQIYELQQKYPEATAELEKAHAAAPEDGEITYGLGQAYALVGRKDEALKIANELNQP
                     GKQNVYLPKEAAYLYSLLGEKEQAVAILQKAAENHYLAVAELKMDPRLTELRKDARVV
                     ELLRRIGLGQ"
BASE COUNT          909 a          912 c          962 g          736 t
ORIGIN      
        1 tggaagaggc gtccagggaa ttggagaagt tgtttcgaga agagtaaaaa accattctcc
       61 agatgtcatt tctcattcgc gaattcaatt tggaagtgca atgagctgaa gttttggaat
      121 cgtctgcttc tggagcgcca aaggcgcgaa tttaatagcc aggggcaagt cccgccttgt
      181 cgggacgccg cccctggaaa ggaagaaaaa gtgtccgagc tctgaaagag gggcattacg
      241 tctcagccac agaagtattg cgccgatcgg ttttggccaa actttacgat cagaactttt
      301 gttgtgtaca ctgcgctcac atcatttacg gaagcccgct tggctcagtg gtagagcact
      361 cgcttcacac gcgaggggtc agtagttcga atctactagc gggcaccaaa aaaacagggg
      421 gcagtagaaa gtagacagta gacagtaggc agagattaac ttgcctaaga tctccgatgg
      481 gaatgcgagg catagcctcg catttttgat tgaggtgccc ttgccggtaa ggcaagcctt
      541 accgcacgga agacggcaga gccgttagat tgtatgaggc cctctcctca tggggttgac
      601 actgcgacga ttgcacgtta ccaagtctca gcgatttacc agcccagccg cagacagtac
      661 gcagtgccgt catcacgccg atgattcctg aatccatagc gcactatcga ataatcaaaa
      721 agttaggcgc aggcgggatg ggtgaagtct atctggcgct ggataccaag ctcgatcgca
      781 aggtcgcaat caaaatcctg cacccggacc agctcgccga agaacacctc aagaaacgac
      841 tagtcagaga agctcaagcc gccgccaagc tcgatcatcc caacatctgt gccatctacg
      901 acgtcaacga ggcaaactcg ttcaccttca tcgtgatgca atacatcgaa ggtgaattgc
      961 tcgccgaaaa gatggaacgc caaccattaa cactgagcac tacgttggct atggtcgagc
     1021 aggctgcgga aggcttggca gaagcgcacg ctcagggaat tgttcaccgc gatatcaagc
     1081 cgcaaaacat gatcgtcacg ccgcgcggcc agttgaagat cctggatttc ggtctggcca
     1141 agcaactgca ccaaagcggt accgtggaca atgaagcggc gacagccacc ctcttgagca
     1201 agccgggact cgtggttggg accatgcctt atatgtcgcc ggagcaggtg aagggtgaac
     1261 cacttgatgc gtcaagcgat atctttagcc tcggcattac tctttatgaa atgctggcgg
     1321 ggaaacaccc gttcaaggac aagagcgcgg ccgtgacaat gtcccggatc ttgctcggcg
     1381 aacccattcc caccgagaaa tttcaaactc aagtttcgcc ggagttacaa gctctcttag
     1441 gcaagatgct gcgcaaggac aaagcggagc gctatcaaaa cgcccaggat ttccttaccg
     1501 acttaagaca actgcctgtc ccgcctccag ggcatgagac tcaggcaaac gccgggcgta
     1561 ctcaagaggc ttcagcgatc aggcagccgg aaaaagcagg cgacagtttt ctcagcaagg
     1621 cccggcgcaa taagtggccg ctcctggcgt cggtgcttgc gctggttctc atagcagtgg
     1681 gaattagcac gtggctctcg aaagagcact cggattcttt agcgatcctg ccgttcacct
     1741 acacgtccag cgacccgcaa ctgatggcta atcccgatcg cgagtactta agcgatggca
     1801 tgactgagag catcatcaac aacctgtcac agttgaccaa cttgaaagtg atcgcgcgta
     1861 gttctgtctt tcgctacaag ggcaaggaca tggatgtgca ggcgatcggg cgagagctga
     1921 atgtacgaac cgtgctggtt ggcaagatca ttcaagaagg tgaagggtta agaattgtag
     1981 ttgatttgat ggacgtgcaa cggaacagcc agatctgggg cgagaggtat gagcgaaaaa
     2041 cctccgacat tcagtctgtc caaagagaaa tcgccactag agtttccgaa aaactgggtt
     2101 tgaaattaac gggcgcggat cagagtcaac tcgcaaagtc ttacactaat agcggcgaag
     2161 catacgagtc ctatttgaag ggacgctatc actggaataa gcgcacggat gaaggcttca
     2221 agcaggcgat ccagttcttt cgcgaagcta ttgataaaga tccaacctac gcgttggcgt
     2281 atacaggact tgcggattgc tacacgctgc aaagtgatta tggatttatc ccggcgcagg
     2341 aaggctacgc tttggcgaaa ggtgccgtca ccctggcact caaatacgac gatagtctcg
     2401 ccgaagcgca cacttcgttg gcctcaatca aggctgctac tgattgggac tggcaaggag
     2461 ccgagaatga atatcgccgg gcaatagagt tgaatcccaa atatgcgact gcccaccatt
     2521 ggtatggcgc acagttgttt ctccaaggca gattcgacga ggcactgccg caactcagaa
     2581 cagcgcaaca gctcgacccc ctctcgcttg gcattaataa ggacttggcg gttgccctgc
     2641 tttatgcgcg cgattatgag aaagcattgg aacagtgccg caagactctg gagatagagc
     2701 cgagctttca cgtaatgtcg acttacatgg cgcagatcta tgaactacag cagaagtatc
     2761 ctgaggcgac cgccgaactg gaaaaggcac acgccgcagc tcccgaagat ggcgagatca
     2821 cctatggact gggtcaggcc tatgcgcttg tgggaaggaa ggatgaggcg ctgaaaatag
     2881 caaatgagct gaatcaaccc ggcaaacaga acgtttacct gcccaaagaa gccgcctacc
     2941 tctattcact tctgggtgaa aaagaacagg ctgttgcgat cttacaaaag gctgcggaga
     3001 accactacct ggccgtcgcg gaactgaaga tggacccccg cctcactgag ttgcgcaagg
     3061 acgctcgcgt agttgaactg ttgcggagga ttggattggg gcaatgactt ccgcggacag
     3121 aagcaagctt tcgcgaggcc cgcagggccg ccagtcaata gccacgcccg tgagggcgtg
     3181 gtcaccttgc gaacatcttt gtggaggccc aaagggccga cactcaatgg tgccggcgct
     3241 tcgcgcctcg aagaagagaa tcaaacgtga tccacgccct cacgggcgtg gctactgact
     3301 aacggccctt cgggcctcac acaaattgaa gaccgcgaag ctgaacagcg aactgatcaa
     3361 cttcgcggtc ttgaataggg gaagtgcctc gacttcaggc tcggctatct ctttttcgca
     3421 gctttcttct ttgcggcttt cttgggagca gccttctttt tggtcgactt tttcttcggc
     3481 tcaccgcctt taatggacat tgaagtctcc tctctgttg
//
LOCUS       DASUEX010000587         2943 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1356473, whole genome shotgun sequence.
ACCESSION   DASUEX010000587 DASUEX010000000
VERSION     DASUEX010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2943
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1356473"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="VFV61_09770"
     CDS             complement(<1..500)
                     /locus_tag="VFV61_09770"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4900809.1"
                     /translation="MQSAETIGSLDDLALPQRWRALTMLAAAQLLAMSLWFSGAAVLP
                     ALRSEWTLSESTAGWLTIAVQLGFVAGTLLSAFLNLPDVFSPRHLVASCAVLGAAANA
                     AFGCFAEGPRLAIPLRFLTGMFLAGVYPPGMKILATWFRRSRGMALGVLVGALTLGKA
                     SSYLING"
     gene            complement(751..2454)
                     /locus_tag="VFV61_09775"
     CDS             complement(751..2454)
                     /locus_tag="VFV61_09775"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX4900810.1"
                     /translation="MTRAKKRELLRSAYMWLVTTAGAVTVAISAYTLPTAQIDRRFIV
                     LGLAMLIGSRITIEIPRAKGHISVSDTFVFLTLMLFGGEAAVLLAATEALTSSNRFSK
                     KPIVALFNAGVMACSTGLTATVVHFGFGPLTQLSNSYSPNLIVALCVMALLQYVLNSG
                     LVAISAALKLDQPVWFTWRTNFLWTSITYFAGASAAAIVTQLMSQIGFYAFLALIPII
                     AIIYLTYQTYLRNVETSAAQAEQAKLHVEELNRVIAEQERISKALQESEEHFRTAFDY
                     AAIGMALVSTAGSWLRVNHSLCDIVGYSETELLESDFQAITHEEDLGNDLAQIYRMLS
                     GELHTCQVEKRYLHKDGHDVWALTSISLVRDAQGSPLHFIFQIEDVTERKRAEGAIKT
                     LSLVDELTGLYNRRGFLAFSEQHLISLQRANKSVMVVYADLDGLKQINDSYGHKEGDR
                     ALMKTAEILHETFRSSDVLGRLGGDEFTIFAAVEPEGGVETLIARLNDKFQKYNSQST
                     SPYRLSVSVGLAFMHPDECYTVEDLMAQADESMYQNKRKKKSGVAWREMEVQRFTEAV
                     A"
BASE COUNT          707 a          817 c          768 g          651 t
ORIGIN      
        1 ccattaatca ggtaggaaga cgctttaccc agcgttaatg cgccgactaa aactcctagc
       61 gccatcccgc gcgaacgtcg gaaccaggtc gccaggatct tcattcccgg aggatagact
      121 cccgccagga acattccggt cagaaagcgc aggggaattg ccaatcgcgg accttccgca
      181 aagcatccga aagcggcatt ggcggcggct cccaatactg cacaactcgc aaccagatgt
      241 ctgggactaa aaacgtcagg taggttgaga aaggcactca ggagcgtgcc cgccacgaag
      301 ccaagttgca ctgcaatcgt taaccaacca gcggttgatt cgctcagcgt ccattcagat
      361 cgcaacgcgg gcaacactgc agcgccactg aaccaaagcg acatggccag cagttgggcg
      421 gcggccaaca tggtgagggc ccgccagcgc tgcggcaatg ccaggtcatc cagagaaccg
      481 attgtttccg cgctctgcat cggcggcgcg caggatacta caaaacaaaa taggcaggtc
      541 ggggaacctg ccacttacaa aaacaatcgg aggcgctgtt gagcagcctc cgattaatac
      601 gaaaccggga ccggtagcga ccgggttaaa tactcaactt ggactttggc ttgataaacc
      661 ttgcataccc cctggaatgc ttcgatccaa gacccggacg ctaccgctct cggttcgcat
      721 ttgtcgccca tgaccaggca gtgcaagccg ttaggcaacc gcttccgtaa agcgttggac
      781 ttccatttcc cgccaggcga cgcccgactt cttcttccgt ttgttttgat acatcgactc
      841 gtcagcctgc gccattaagt cttccaccgt gtaacattca tcgggatgca tgaatgccaa
      901 gcccacgcta accgaaagcc gataaggcga ggtactctgc gaattatatt tttgaaattt
      961 gtcgttcaga cgggcgatga gcgtttctac gccaccttca ggctcgactg ccgcgaagat
     1021 ggtgaactca tcgccgccca agcggccaag cacgtcggat gagcgaaatg tttcgtgcag
     1081 gatctcggcc gtcttcatca gcgcccgatc accttcttta tgaccatacg aatcgttgat
     1141 ctgtttcagt ccgtcgaggt cggcatagac taccatcaca ctcttattgg cgcgctgcag
     1201 tgagatcaaa tgctgctcgg aaaaagcgag gaacccgcgg cggttataga gtccggttag
     1261 ttcatcaacc agcgaaagtg tcttgattgc accctcggcc cgcttacgct cagtgacatc
     1321 ttcaatctga aaaatgaagt gcaacggcga accttgcgcg tcccgcacca gcgagatgct
     1381 cgtcagcgcc cacacgtcgt gtccgtcctt atgcaggtaa cgcttctcta cctgacaggt
     1441 atgaagttcg cctgacagca tccggtagat ctgagccaga tcattgccga gatcctcttc
     1501 gtgagtgatc gcctggaagt ctgactccag cagttcagtt tcactgtagc caacgatgtc
     1561 gcacaatgaa tgattgacgc gcagccaact gccggccgtc gaaaccaacg ccataccaat
     1621 ggccgcgtaa tcaaaggcag tgcggaaatg ttcctcgctc tcctgaaggg ctttgctgat
     1681 gcgttcctgt tcagctatga cccggttcaa ctcttcgacg tgaagcttcg cttgctcagc
     1741 ctgggcggct gacgtctcga cgtttctcag gtaagtctgg taagtgagat agatgatggc
     1801 gatgatcggg atcagagcca ggaacgcgta gaagccaatc tggctcatca attgagtaac
     1861 aattgcggcg gcggatgcgc cggcaaagta agtaatggag gtccagagaa aattggttcg
     1921 ccaggtgaac cagacgggct ggtcaagttt caacgcggca ctgatggcca ctaatccgga
     1981 gttgagtacg tactgcagca gagccatcac gcaaagcgca actatcagat taggagaata
     2041 gctatttgat aactgggtta agggaccaaa tccaaagtgc acgaccgtgg ccgtcaatcc
     2101 cgtcgaacac gccattactc cggcattgaa gagagccaca atcggctttt tgctgaaacg
     2161 gttcgaagaa gtgagggctt ccgttgccgc caggagaacc gccgcctcgc caccgaagag
     2221 catcagcgta agaaaaacga aggtatcgga aactgagatg tgtcctttgg ctcgcggaat
     2281 ctcaatcgta attcgtgagc caatcagcat cgcgagtccc agcacgatga agcggcgatc
     2341 gatttgtgcc gtcggcaggg tgtaagcaga gatagccacg gtgactgcgc cagcggtcgt
     2401 caccaaccac atgtaagcgc tccgtagcaa ctctcttttc tttgctcgcg tcatcgtcgg
     2461 gtgttcagta aaaagctcct atcagcactg tgcggtacca tgcaccgcat cgcagcttcc
     2521 tagtgcaacg actattccgc acggtacctc gtcaaatcgg gcttgaattg tggccttttt
     2581 actagccgat tcccttgctc ggtcaacaag cagtcattct gaagactgac gctattcaat
     2641 ctgacagaag cagagaacga gccatgtttt gctggggggt gtcacgcctt ttcggacgcg
     2701 aaactctcat tccggtttaa gccctgacgg ttactacgct gttaattcgg aactggtact
     2761 gcgataaata gctgctactt tgattcgtga tattctgacg tcccccaata aatcgcgacg
     2821 gtcgctatct catttcttct cgacaagggt tggaatttct atgtctgtgg ccgaggtttt
     2881 ggcatcactc acgggaagct cctcaagttt cgcaatcggc cagccgattc gcgaagagtt
     2941 gtt
//
LOCUS       DASUEX010000588         4145 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_315219, whole genome shotgun sequence.
ACCESSION   DASUEX010000588 DASUEX010000000
VERSION     DASUEX010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4145
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315219"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..660)
                     /locus_tag="VFV61_09780"
     CDS             complement(<1..660)
                     /locus_tag="VFV61_09780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900811.1"
                     /translation="MTILRLLLAGFLISQFIPSPAQTSSAGKSPAAELSGLWVAKQRF
                     GPEIRGTLLIRQVANRWSAEISGQTVEPRVEGQSINFTLADGQSRFVGKFDSRRLNIL
                     GHWIQPGTPEVGSVASPVTLTKQGSGWQGTVTPLDSAMTFYLSISERQDGSVGAFLKN
                     PERNIGVNQYPVDHIEREADAIKLFSVAKAGAPPRLLAEGSYDKERELLSVYFANRGG
                     SY"
     gene            complement(810..1484)
                     /locus_tag="VFV61_09785"
     CDS             complement(810..1484)
                     /locus_tag="VFV61_09785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900812.1"
                     /translation="MTKRIGLLLILILFGSGVMAQQRRQSGSQPPADVPGAPSAPSQP
                     TAAPEAANLPDQPPSPPTPPAQPSQQAQPTVPPQPAAPSRRPVPNVPGIPSIPNVPPA
                     DPLGDVMFPPDLILGHSREIMLTAEQKSFMRGEVQKTTSRFTELQWALHDAMEDLHQA
                     LESSSVNDQQALTLLDKVLDIEREIKRLHFGMGIRLKNKLTPEQQAKLQSLRMGRPWA
                     VGQNPG"
     gene            complement(1481..2017)
                     /locus_tag="VFV61_09790"
     CDS             complement(1481..2017)
                     /locus_tag="VFV61_09790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900813.1"
                     /translation="MKLTGSELRIQAQFLELKADDERLTPNFVAIWTQAMTKAERRRP
                     SLRFSYAGVGFVILALFSLVLWSRSWQKQELVVLESPLPPSYLLPTVTNVIQGDEPAR
                     PFRPLPRKHRPAHRRSQLLRQQAVINNASAFAVWQSPTSKLLESPAENVLSALPQLTQ
                     ASLELQSFLPAAELKENK"
     gene            complement(1971..2564)
                     /locus_tag="VFV61_09795"
     CDS             complement(1971..2564)
                     /locus_tag="VFV61_09795"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX4900814.1"
                     /translation="MTTNDWDNERMELAELKPELERLHVASFGWALSCCQRDRAEAED
                     VLQSVYLKILQGKARYRGEANFKTWLFAVIRKTALGERRAQVRRAWRAVVGADRAVAR
                     GRFAAGPLAGLVQAETQTRFLRALDKLPQRQREVLHLVFYEDLSLREAAGVMGVTIGS
                     ARQHYDRAKKHLREHLDREEIEHEAHWQRAENSSAIS"
     gene            complement(2825..>4145)
                     /locus_tag="VFV61_09800"
     CDS             complement(2825..>4145)
                     /locus_tag="VFV61_09800"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX4900815.1"
                     /translation="VLAQLNDISQHAFASPYQRALIHVRLNERDTALSLLREAYVVND
                     GWLVWLGVEPELDPLRGEPEFEELLIKTRNPAVGRDVISAQAAAAAVAKRRSELKVTT
                     APVTSPVPVPAEQTHAGINEEARRLYTAGRYYSTRRSADGLKQAIERLERAVELDPDY
                     ALAHSELADCYALLNWFIEPPPAEAWQRAKESATRAVAADPELAEAHASLGFVRLHYD
                     RDWADAERELRKAILLKPTNQVAHRWYAYSLSAMGRHDEAVVEIERARDISPQSPVIA
                     TAVANVLFLAGRYDEAITQCLKALDLDRGAVAAHTILRWAYEKKGMHNEALATFEQER
                     SFAGDTPTTRAKQAHVLAAVGRHDEAREMLEGILARRQEEWVTAYEIAIIYAWLGDNI
                     NALRWLSQAEREHAVGFTFVRVDPHLETLRSDPHFRDLLRGTDKTIP"
BASE COUNT          887 a         1179 c         1149 g          930 t
ORIGIN      
        1 gtaactgccg ccacggtttg caaaataaac tgaaagtaat tcgcgctcct tatcgtagct
       61 tccctcagca agtaaccgag gcggcgcgcc agctttcgcc acggaaaaaa gttttattgc
      121 atcagcttca cgctcgatgt gatcgacagg atattgattc actccgatgt tgcgttcggg
      181 gttcttgaga aatgctccga ccgaaccgtc ttgccgctcg ctgatagaca ggtagaacgt
      241 catcgcgctg tccagaggcg ttacagttcc ctgccaacca ctgccttgct ttgtgagagt
      301 cacgggtgag gcaacggagc ccacttcggg ggtgccaggc tgaatccagt ggcccagaat
      361 attgagacgt cgagaatcaa acttaccgac gaaccggctt tgcccgtcgg ccaaagtgaa
      421 gtttatcgac tgtccttcca ctcttggttc aacagtttgc ccggaaatct cagccgacca
      481 acggttggca acctgcctga ttagtaatgt cccccggatt tcagggccga atctctgctt
      541 cgctacccat aagccgctga gctcggcagc gggagatttt ccagcgctag atgtttgcgc
      601 aggcgatggt atgaattggc tgatgagaaa gccggccagc aataaccgaa ggatagtcat
      661 agaacattgc tccgatggtc tcatgagccc gacaagatca tcgaggatcg gacttcagct
      721 cgagattcct cacagagaaa acaagcgagc cggggaaaag ttactaacgc ggggagaatc
      781 cctgattatc tgatttgcct caaaggagct cagccaggat tctggcccac tgcccagggg
      841 cgtcccattc gcaggctttg tagttttgct tgctgttcgg gagtcagttt gttttttagc
      901 cgaatgccca tgccaaaatg caaacgtttg atctccctct caatgtccaa aaccttatct
      961 aacagagtga gggcctgctg gtcattcacc gaactggact ccaacgcttg atgaagatct
     1021 tccatcgcgt cgtggagcgc ccactgcaat tcagtaaacc gactagtggt cttctgcacc
     1081 tcgccgcgca tgaaactctt ttgctcggcg gtgagcatga tttcccgcga gtgccccagg
     1141 attagatcgg gtggaaacat cacatcgccc agcgggtcag ccggagggac attgggaatg
     1201 ctgggaatac cgggcacgtt cggtactggc ctcctgctcg gcgccgctgg ttgcggtggc
     1261 accgttggct gcgcttgttg cgagggttgc gctggagggg ttggcggaga cggtggttgg
     1321 tccggaaggt ttgcagcctc tggcgcggca gttggctgag acggcgcact cggcgcacca
     1381 gggacatcgg ctggcggttg gcttcctgat tgacgtcgtt gctgcgccat cactcccgaa
     1441 ccgaacagga tgagtatcaa caacaatcca attcttttag tcatttgttc tccttaagtt
     1501 ctgcggccgg caggaaagac tgcaattcga gggaagcttg agttagttgt ggcaaggcgg
     1561 ataacacatt ctcggccggc gactcgagca gcttactagt aggagactgc cagacagcga
     1621 atgcgctggc attgttgatg acagcttgct gtcttaggag ctgcgagcgc cggtgcgcgg
     1681 gacgatgttt tcgcggcaga gggcgaaacg gtcgggccgg ttcatcccct tgaatcacat
     1741 tggtaacagt ggggagcaga taagaaggtg gtaacggcga ttcgagcact accagctctt
     1801 gcttttgcca gcttcgcgac caaagcacca gcgaaaacaa cgccagtatt acaaaaccca
     1861 caccagcata cgaaaatcgt agcgatggac gtcgccgctc ggcttttgtc atcgcttggg
     1921 tccaaatagc gacaaagttc ggcgtcagcc gttcgtcatc agccttcaac tcaagaaatt
     1981 gcgcttgaat tctcagctcg ctgccagtga gcttcatgtt caatttcctc gcgatccaaa
     2041 tgctctcgca aatgcttttt cgctctgtcg taatgttgtc gagcactgcc gatcgttacg
     2101 cccatgactc cagcagcttc gcgcaagctc aagtcttcat aaaaaaccaa atgcaatact
     2161 tcacgttgac gctgcggcag tttgtccaac gctcttagaa atcgagtttg cgtttccgcc
     2221 tggactaatc ctgccagagg tcccgctgca aaacgtcctc gcgcaacggc acgatcagct
     2281 ccgacaactg ctcgccaggc gcgccggacc tgagcgcggc gctcacctaa agctgtcttg
     2341 cgaatcactg caaagagcca ggtcttgaag ttggcttccc cacggtagcg tgctttgcct
     2401 tgaagaattt tcaaatatac cgactgtagg acatcctccg cctcggcgcg atcgcgctgg
     2461 caacagctaa gggcccagcc aaaactggcg acatgaagtc tttcgagctc cggcttcagc
     2521 tccgcgagtt ccatgcgttc gttgtcccaa tcattagtcg tcacggcggg cgaattatat
     2581 gaccggtcgg agctcaaacc tcagctaatt accgccaagg cagccaagag acaccaagcg
     2641 cgcctggaat tcttaggcta tgcagttgaa tccaatctct cgattctgag cgtgactttt
     2701 ctcgcttggg cgagtcttgt tggcttggcg gctagtgttt aggcggtact gagccggcct
     2761 agcctggagc agttggggaa ggttatcaat cgacaatcga caatcggcat ttggcaatcg
     2821 gcaattatgg gatcgtcttg tcggtgccgc gcaataagtc tctgaagtga ggatcggatc
     2881 ggagtgtttc caaatgggga tcaacgcgca caaaggtgaa tccgacggcg tgttcccttt
     2941 ccgcctgaga taaccaacgt agtgcattga tgttgtcgcc cagccaggcg tagatgatcg
     3001 caatttcgta agccgtgacc cactcctcct gccgccgcgc cagaattcct tccagcattt
     3061 cgcgcgcttc atcgtggcgc ccgaccgccg ccaggacatg ggcttgcttt gcgcgtgtgg
     3121 tcggcgtatc gccggcaaag ctacgttcct gttcaaaagt cgccagggct tcgttgtgca
     3181 tgcccttctt ttcataagcc caacgcaaga tggtgtgcgc agcgaccgca ccgcgatcca
     3241 gatcgagcgc cttaagacac tgggtgatcg cttcgtcgta acgtcctgcc agaaacagca
     3301 cattagccac agccgtggcg atcaccggag attgcggcga gatgtcgcgc gcgcgctcaa
     3361 tttcaacaac agcttcgtcg tggcgcccca tcgccgagag gctgtacgca taccagcgat
     3421 gcgcgacttg gttagtgggc ttgagcagaa tcgccttacg cagctcgcgc tcggcgtcag
     3481 cccagtcgcg atcgtaatgc agtctgacaa acccgagtga tgcatgcgcc tccgccaact
     3541 ccggatccgc cgccactgcc cgtgtggccg attcttttgc tcgctgccag gcctcagccg
     3601 gaggcggttc gatgaaccaa ttcagcagcg cgtagcaatc ggctagttct gagtgagcga
     3661 gcgcatagtc tggatcaagc tccacggcac gctccagtcg ctcaatcgcc tgcttcaagc
     3721 catcagcgct gcggcgcgtc gagtagtaac gaccggcagt gtagagccgg cgcgcttctt
     3781 cattaatacc cgcatgcgtc tgctcggccg ggacgggaac cggtgaagtc acgggcgcag
     3841 tcgttacctt tagctcgctg cgacgcttgg cgacggcggc ggccgccgct tgcgctgaga
     3901 taacatcacg acccacggcc gggttacgcg tcttgattag cagttcttca aactcgggct
     3961 cgccgcgcag cggatcaagt tctggttcca ctcccagcca aaccagccag ccgtcattca
     4021 ccacgtaagc ctcacgcagt agcgatagcg cagtgtcccg ctcgttcaag cgcacatgaa
     4081 tcaatgctcg ttgatagggt gaggcgaaag cgtgctgcga gatatcgttc aactgcgcaa
     4141 ggact
//
LOCUS       DASUEX010000589         2830 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_103388, whole genome shotgun sequence.
ACCESSION   DASUEX010000589 DASUEX010000000
VERSION     DASUEX010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103388"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="VFV61_09805"
     CDS             <1..292
                     /locus_tag="VFV61_09805"
                     /inference="COORDINATES: protein motif:HMM:NF012911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEX4900816.1"
                     /translation="QSMINRTIARKFVIRGDVQGVGYRFFAQRAAARHQVVGYVRNLS
                     DGSVESLAEGTPASVEAFKHDLVTGPQWAAVSQVEELVLEPSNLYSSFRIER"
     gene            431..952
                     /locus_tag="VFV61_09810"
     CDS             431..952
                     /locus_tag="VFV61_09810"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF002634.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEX4900817.1"
                     /translation="MNHLRELIREVPDFPKPGINFYDITTLLKHPEGLKNTVDALAAE
                     YEGTRVDAVLGVEARGFIFAPALAYHLGTGFVPVRKPRKLPAECASISYDLEYGQDTL
                     EIHRDAIGDGHRVLIADDLLATGGTARAVVDLVEQLGGEVVGLVFVVELEFLSGRKKL
                     DGYEVKSLIKYQS"
     gene            990..2675
                     /gene="sulP"
                     /locus_tag="VFV61_09815"
     CDS             990..2675
                     /gene="sulP"
                     /locus_tag="VFV61_09815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate permease"
                     /protein_id="HEX4900818.1"
                     /translation="MHKRLEPKIFAVFREGYSRKQFYSDLAAGTTVGVVALPLAIAFA
                     IASGVKPEQGLYTAIIAGFLISLLGGSRAQISGPTGAFVVIIYGIVQQYGYDGLAVAT
                     LIAGLLLMLMGLFRMGALLKFIPYPVTVGFTTGIALIIFSSQIGDFLGFHLVSPPAGF
                     IERLVAYREHISELNPYAFIVGLISLLVIGIWPKVTHRVPGMLIAIIVATLAVKLFHL
                     PVETIGSRFGAVPNRLPAPHLPLVSLTLVKQMFAPAVTIAFLAALESLLAAVVADGML
                     GTRHRSNMELIAQGAGNIGSVIFGGIPATGAIARTATNIKSGGRTPVAGMIHAIVLLL
                     ILLFLGKYAALIPMPALAAILIVVAYNMSEWKGFRDLLAGTRSDAVVLVVTFLLTVVI
                     DLTVAIQVGVVLAAFLFMQRMSNTTEVTKVTETLMDEDESETRDMSKLEVPANVEVFE
                     IYGSLFFGAIERFKDAMRSVEKRPRVLILRMRHVQTIDASGLHSLKELLESTKRRGIV
                     LIISAARPNVRALMERTNFAEALGEQNFCEDIFCALDRARLILAERDMHATSG"
BASE COUNT          601 a          715 c          769 g          745 t
ORIGIN      
        1 gcagagcatg attaatcgca caatagcgag aaaatttgtg attcgcggtg atgtgcaagg
       61 cgtcggttat cgtttttttg cgcagcgggc agcggcccgc caccaggttg tcggttatgt
      121 acgcaatttg tcggatggga gtgtggagtc gcttgctgag gggacgccag ccagcgttga
      181 ggctttcaaa catgatctgg tcactgggcc acagtgggcg gctgtatcgc aggttgaaga
      241 actagttctc gaaccttcga atttgtattc ttcatttcgg attgaaagat gaaacttcga
      301 actttgtact tagaagtttc cgctttgttt gtgttatgaa tcttgagtcg tacaaccaat
      361 aagtttcagg ttccaggttc caggttcaaa gttcaaagtt caaagttcaa agaccaaagt
      421 tcaaattcca atgaatcact taagagaatt gatcagagaa gtccctgact tcccgaaacc
      481 cggcattaac ttctatgaca tcaccactct cctaaagcat cctgagggtc taaagaacac
      541 tgtggatgct ttggctgctg aatacgaggg cacgcgcgtg gacgctgtcc tcggcgtcga
      601 agcgcgcgga tttatctttg ctcccgcgct cgcttatcac ctcggcaccg gctttgtccc
      661 ggtgcgcaag ccgcgcaaac tccctgccga atgcgcctcc atttcttatg atctcgaata
      721 cggccaggac acgctggaga ttcatcgcga cgcgattggc gatggtcacc gcgtgttgat
      781 tgccgacgac ttgctcgcga ccggcggcac cgccagggcg gtggttgatt tggtcgagca
      841 actcggcggt gaagttgtgg gactggtgtt tgtggtggag ttggaattct tgtctgggcg
      901 caagaaactc gacggttatg aagtgaagtc gctgatcaaa taccagtctt agttagttac
      961 cacttcgtac gtgtcggcag tcacccttaa tgcataagcg gctcgagcca aaaatatttg
     1021 cggttttccg cgaaggctat tcccgcaaac aattctattc agacctcgca gctggtacca
     1081 cggtcggcgt tgtcgcactt cctctcgcca tcgctttcgc gattgcttcc ggagtaaagc
     1141 cggagcaagg cctctacaca gccatcatcg ctggttttct aatttcgttg ctgggcggat
     1201 cgcgcgcgca aatcagtggc ccgactggcg cgttcgtagt gatcatttac ggtatcgttc
     1261 agcaatacgg ttacgatgga ctcgccgtcg caacgctaat cgcggggttg ctcctaatgt
     1321 tgatggggct tttccgtatg ggggccctgc tcaaatttat tccttacccg gttacggtcg
     1381 gattcaccac cggtatcgct ctaataatct tctcgtccca aattggcgac tttcttggct
     1441 ttcacctggt ctcaccgccg gctggcttca tcgagagatt ggttgcctac cgcgagcaca
     1501 tctcggagtt gaatccgtat gctttcatcg tgggcttgat atctcttctc gtgatcggga
     1561 tttggcccaa agtgactcat cgcgttcccg gaatgttgat tgccattatt gtggccacct
     1621 tagcagtcaa gcttttccat ctcccggttg aaacgattgg aagtcggttt ggcgccgtac
     1681 ccaatcgtct tcccgcacca cacttgcccc tcgtttcgtt aacgctggtg aagcagatgt
     1741 ttgctcccgc agtgacgatc gcatttcttg cggcactgga atcactgctt gcagcagtcg
     1801 tagctgatgg catgctggga acacgccatc gctcaaatat ggaattgata gcgcagggcg
     1861 cgggcaatat tggttctgta atttttggag ggattccggc aaccggcgcg attgctcgca
     1921 cggcaacgaa catcaaaagc ggcggtcgta cgcccgtggc ggggatgatc cacgcaatcg
     1981 tcctgctgct aatcctcttg tttttgggga aatatgccgc gctcattcct atgcctgccc
     2041 tcgctgccat cttgatcgta gtcgcttaca acatgagcga gtggaaagga tttcgcgatc
     2101 tattagcggg aacccggagc gatgcagttg tcctggtagt aacgttcttg cttacggtgg
     2161 ttatcgatct gactgtggcg attcaagtgg gagttgtatt ggccgcgttc ctgtttatgc
     2221 agcgcatgtc gaacacgacg gaagttacga aggtaaccga aacgctcatg gatgaggacg
     2281 aatcagaaac gcgcgatatg tcgaagctcg aagtgcccgc caacgttgaa gtattcgaga
     2341 tctatggatc gcttttcttc ggagcaatcg agcgattcaa ggacgcgatg cgcagtgttg
     2401 agaagcgccc acgcgttttg atactgcgca tgcgccacgt gcagacgatc gacgcgagcg
     2461 gactacacag tcttaaagag ttgctcgaaa gcacgaaacg gcggggcata gtcctgatta
     2521 tttcggccgc tcgcccgaat gttcgggcgc tgatggagcg taccaacttt gctgaagcac
     2581 ttggtgagca aaacttctgt gaggatattt tttgcgcgct ggaccgggct cgtctgatcc
     2641 ttgctgaacg agacatgcac gccacgagtg gttaggtttt aggtggtgac gcaccgaacc
     2701 ctggtgctgg cgatagtcaa aatcggcaat catcaatcgg taattggcaa tgccatagct
     2761 tgacacaccg gactgttggt tgttatacgc agcttctcaa gttttctcga cccataagtc
     2821 ctctttctca
//
LOCUS       DASUEX010000590         2388 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_440137, whole genome shotgun sequence.
ACCESSION   DASUEX010000590 DASUEX010000000
VERSION     DASUEX010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2388
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440137"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1576
                     /locus_tag="VFV61_09820"
     CDS             <1..1576
                     /locus_tag="VFV61_09820"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX4900819.1"
                     /translation="EFYKTKKMKYPPEYGPLAAVTPGTPGGILVMLAEYGKLSLQEVL
                     EPAIQMADGYPIEAQLANTIEREKGRLKEWRYSAEVLLPHRGEKREAPQAGEIFRQPD
                     LAATLRKLVQAEQQALAAGKNRKEAIYAAYDRFYRGDIAREIARGTVEQGGLITVEDL
                     ANWKVHIEEPVKTSYRGIDVYKLTHWTQGPALLQALNILENFDLKAMGYNSSRYIHAL
                     YQTMSLAFADRDFYYGDQYFPPEEPIRGLLSKDYAKKRASQINWERNDALIGPGDPYP
                     FQGQTNPYRGLLEKWTTAPTSAPTSALRPAQNAVRLVTLKAKSRRAEERFKRAFYAGT
                     TSVEAADEEGWVVSVTPSGGWVPAVIAGRSGIGLSQRAQSFVTDPADGPFNVIEPGKR
                     PRVTLTPTLAMKDGKPFLAFAVQGGDSQDQNLLQFFLNVVEFGMTVQQAAEAANINSF
                     QMRSSFADHESRPGRILLAESMPPWVRAELRRLGYDLTFEERTSGPINAIQFDRTHGT
                     MWGGSSNHGEDYGVAW"
     gene            complement(1616..>2388)
                     /locus_tag="VFV61_09825"
     CDS             complement(1616..>2388)
                     /locus_tag="VFV61_09825"
                     /EC_number="1.14.16.1"
                     /inference="COORDINATES: protein motif:HMM:NF012571.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phenylalanine 4-monooxygenase"
                     /protein_id="HEX4900820.1"
                     /translation="PVFAIRNTRTRRNAIAEMALDYQTGAPIPDAPYTREEHFLWKSI
                     RDALEPAQQQHACAEYLDCVRKLELPRDRIPQLREVSEKIQAISGFRLEPVAGLVEPR
                     VFLESLADGVFLCTQYIRHHSTPQYTPEPDVVHEITGHAVTLASDRLAELNRLVGDAV
                     KRTQSAEALERLSRVYWFTIEFGVLLEGGELKAYGTGLLSSAGELEEMHRAELRPLDL
                     DMASGEKYDPTRYQPILFCAESFQEMYRALRAFLVSWP"
BASE COUNT          562 a          650 c          673 g          503 t
ORIGIN      
        1 cgagttctac aaaacgaaga aaatgaaata cccgcctgag tatggcccac tggctgccgt
       61 aacaccggga acgccgggcg gcattctggt aatgctcgca gagtatggaa agcttagttt
      121 gcaggaggta ctcgagcctg cgatccagat ggccgatggt tatccgatcg aggctcagct
      181 cgcgaacacc attgaacgtg aaaaaggaag acttaaagag tggcgttact ctgctgaagt
      241 tctgcttccg caccggggag aaaagcgcga agcccctcag gcaggcgaga tcttccgtca
      301 acctgatctc gctgcgacat tgagaaagct ggtgcaggcc gagcaacagg cgctcgcagc
      361 cggcaaaaac cggaaggaag ctatctacgc ggcgtacgat cgtttttacc gaggcgacat
      421 agctcgcgag atcgctcgag gcacggtgga gcaaggcgga ctgattaccg tggaagacct
      481 ggccaactgg aaagttcata tcgaagagcc agtcaagact agttatcgcg gtatcgacgt
      541 ctacaaactt actcactgga cccaaggtcc ggcgctgctt caggcactta acatcctcga
      601 gaacttcgac ctcaaagcga tgggctataa cagctcccgc tacatccacg cgctctatca
      661 aacaatgagt cttgcttttg ccgatcgcga cttctactac ggggatcaat acttcccacc
      721 agaggaaccg atacgcgggt tgctatctaa ggattatgcg aagaagcgcg ccagtcagat
      781 taattgggag cgtaacgacg ctctcatagg gcccggagat ccttatccgt ttcaaggtca
      841 aacgaacccc tatcgcggac tactggaaaa gtggaccaca gcgccaactt cggctccaac
      901 atccgcgctg cgcccggctc agaatgccgt tcgacttgtc accctcaaag cgaaatcccg
      961 ccgggcggag gaaagattca agcgagcgtt ttatgccggg acaacttctg tggaagcagc
     1021 ggatgaagaa ggatgggttg tttcagtaac tccaagtggt gggtgggtac cggcggttat
     1081 tgccggtcgc agcggcatcg gcctgagcca acgggcgcaa agctttgtca ctgacccggc
     1141 agacggcccc tttaatgtta tagagcccgg caagcgtcca cgcgtaacgc tcacgcctac
     1201 gctcgcgatg aaggacggca agccgtttct tgcgtttgca gtccagggcg gcgacagcca
     1261 ggatcaaaac ctcttacagt ttttcttgaa cgtggtcgag tttggtatga ctgtccagca
     1321 ggctgccgag gcggcgaaca tcaacagttt tcaaatgcgc agctcatttg ctgatcacga
     1381 atccaggccg gggcgaattc tgcttgcgga atccatgccg ccctgggtgc gtgctgaatt
     1441 gcggcgcctg gggtatgacc tcacgttcga ggagcgcacc tccggaccga tcaatgcgat
     1501 tcaattcgac cgcactcacg gcacaatgtg gggcggatca agtaatcatg gagaagacta
     1561 cggcgttgcc tggtagaccg gtcttcgcca ggaatgcact cggtgtttgt tctcatcagg
     1621 gccagctaac tagaaaggca cgaagggctc ggtacatttc ctgaaaagac tcggcgcaga
     1681 agagtatcgg ttgatatctg gttggatcgt atttttcacc tgacgccata tccaggtcga
     1741 gaggacgcaa ctccgcgcga tgcatctctt ccaactctcc ggcagaggat agtagccccg
     1801 ttccataggc tttaagttcg ccaccttcaa gcaagactcc gaactcaatc gtaaaccagt
     1861 agacacggga aagccgttcc agcgcttcag ctgactgcgt ccgcttgacc gcgtctccca
     1921 caaggcgatt gagttcagcg agccggtcac tagctagagt aacggcatgg ccggttatct
     1981 cgtgcacgac atcgggctca ggcgtgtatt gtggcgtcga atgatgccga atgtactgcg
     2041 tgcataggaa gactccatca gccaacgatt cgaggaagac gcgtggttct acgagcccag
     2101 ctacaggctc cagacggaat ccactgattg cctgaatctt ctcagaaact tcgcggagtt
     2161 gtggaatccg atcgcgcggc aactctaatt tccttacgca gtcgagatat tccgcgcagg
     2221 cgtgttgttg ctgggccggt tcaagtgcat ccctaatgct cttccagaga aaatgctctt
     2281 cgcgcgtata tggcgcatcg gggatcgggg ctcctgtctg gtagtctaaa gccatttccg
     2341 cgatcgcgtt acggcgggtg cgggtattcc tgatcgcgaa aaccggga
//
LOCUS       DASUEX010000591         3972 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1484351, whole genome shotgun sequence.
ACCESSION   DASUEX010000591 DASUEX010000000
VERSION     DASUEX010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3972
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1484351"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            413..1405
                     /locus_tag="VFV61_09830"
     CDS             413..1405
                     /locus_tag="VFV61_09830"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEX4900821.1"
                     /translation="MANFSCEHEVTDNLCSASEFRKNEPAVNRWAVILAGGDGTRLRP
                     LTMSISGDERPKQFCPIIGGRTLLEQTRRRVALSVAAKQTVTVVTRTHEVFYKSLLKD
                     VPRGRLLVQPENKGTAPAILLSLLRVAQLSPQATVAFFPSDHFFADDIQFMSHIDSAF
                     TASQKRPELITLLGIKPDKPEVEYGWIEPHSSLFGHLPRGITRVERFWEKPTALVARR
                     LLERGCLWNSFVMVGRVDAFLEMTRRALPELYESFSAAADSLGTASESFVLDELYART
                     VASNFSHEVLALRPEDLSVMQVSDVGWTDLGEPQRVFSTLHTLGVQAQWAMPSA"
     gene            1671..2774
                     /locus_tag="VFV61_09835"
     CDS             1671..2774
                     /locus_tag="VFV61_09835"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEX4900822.1"
                     /translation="MKAIAVFPGKPNTVHLTEMPKPAVDEIAGGRGVLVKVLRVGVDG
                     TDKEINAAEYGKAPAGYDFLVIGHESFGQVESVGPNVSEVKPGDYVVATVRRPGSSIY
                     DLIGTNDMTTDDTYFERGINLRHGYMSEYYVDDAEFIVKIPQGLKHVAVLLEPMTVVE
                     KGIVQAYEIQRRLKVWRPRRAAVLGAGTIGLLATLVLRLRGLEVTTFGLNPKPYLNSD
                     LIEGIGCRYESTRDLPLLDGAKKYGPFDIIFEATGFSPMVFDGMQALGKNGVLVLSSV
                     TGGDRKIEVPADKINLEFVLGNKVMVGTVNANRDYFEAGVKDMAQAEAEYPGWLGRLL
                     THPVKGLENYQELFAKLSNGGGAIKVFCEVAPL"
     gene            2728..>3972
                     /locus_tag="VFV61_09840"
     CDS             2728..>3972
                     /locus_tag="VFV61_09840"
                     /inference="COORDINATES: protein motif:HMM:NF023855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching enzyme N-terminal
                     domain-containing protein"
                     /protein_id="HEX4900823.1"
                     /translation="MAAVLSKCFAKSHRYSRTDAGLEGRMIKVNEQDCRNLDKALERE
                     WLETNGLGGFASATIAGANTRRYHGLLTAATKPPVGRVVMLSKLEETFVSGEVRYELA
                     TNQYPGATYPQGFQLLTGFRLDPFPISTFLVAGIEIEKSVFMVSGENTTVVQYDFRLA
                     PFEGDQRATLPKSLAFEIRPLIAFRDYHSTTHENGALNAHVAVEPGCARVAPYADLPA
                     LYFAYDGGDINTNGDWYRNFEYLVEKERGLDFQEDLFNPFTLRFSIDPATLRKKSGVR
                     LSIIASTEIRDSKRAEAYRVAEIQRRTDIVSAVTRGTDLLRTLTLAADQFIVARGEQK
                     TIVAGYHWFADWGRDTMIALPGLTLATGRYEIAKSVLLEFARHVDQGMLPNRFPDAGE
                     EPEYNTVDATLWFFEAVRALVQA"
BASE COUNT          878 a         1035 c         1183 g          876 t
ORIGIN      
        1 acgagtattt ccatggcgat acgggcgctg gagtcggggc gagccatcag accggatgga
       61 ctggcctggt cgcgaagctg atgcagcaga acggcgaaca tcaggagccg gagttggcgg
      121 tggcggtcgc gcaaaacaaa tagactactt ctttgtggac ggccgtaaag gtcgactctc
      181 atgacggctt cgccgcctca catccggcgg caaagccgaa aacgaatctg gcgccagcat
      241 ctttgaggaa atcagtttga aatcaggggc gcccacggag gggcgcccct acaaaatgtc
      301 cgtaaggaat tcacgcatac cgcgactcat catcggaagg aaagtcgagc ggcgcgggca
      361 ggttcggcgg gtccaaaaag cagaccgccc gcgccggtta aggagagcga tgatggcgaa
      421 tttctcttgc gaacatgaag ttaccgacaa cttatgtagt gccagcgagt ttcgcaaaaa
      481 cgaacccgca gtgaatcgct gggccgtaat tctcgcaggc ggcgatggca cgcggctgcg
      541 tccgttaaca atgtcgattt ccggtgacga gcggccaaag cagttctgtc ccatcattgg
      601 cggccgcacg cttctcgagc aaacacgccg tcgtgttgcg ctctcagttg cggcaaagca
      661 aaccgtaaca gtggtgacgc gcacgcacga agtcttctac aagtcgctgc tcaaggacgt
      721 cccgcgcgga cgattgctgg tgcaaccaga gaacaaaggc acggcgccgg cgattctctt
      781 gagcctgttg cgcgttgcgc agttgtcgcc gcaagccacg gtagcgttct ttccctccga
      841 ccactttttc gccgatgaca ttcagttcat gtcgcacatc gattccgctt tcactgcttc
      901 gcaaaagcgt ccggagctga ttacgttgtt gggcatcaag ccggacaagc cggaagtcga
      961 gtatggttgg attgaaccgc actcttcgtt attcgggcac ttgccccgcg ggatcacacg
     1021 tgttgagcgt ttctgggaga agccgacagc cctcgttgcg cgcagactac tggagcgggg
     1081 ttgcctgtgg aatagctttg tgatggttgg gcgcgtcgat gcgtttctgg aaatgacccg
     1141 tcgcgctctg ccggaactct acgaatcgtt ttctgcggct gccgattcgc ttggcacagc
     1201 gtcagaatcc ttcgtgcttg atgagctcta tgcgcggacg gtggcgtcaa acttctcaca
     1261 cgaagttttg gcactgcggc cagaggatct ttcggtgatg caggtcagcg acgtaggttg
     1321 gacggactta ggtgaacccc agcgcgtgtt ctcgaccctg catactctcg gcgtgcaagc
     1381 gcagtgggcg atgccgagtg cttaagctat ggctttagat gcgaggccca gcccatttag
     1441 gaagagcgag gatgacggtg aggcaagcct caccgcacag agggcggcga gccgcaaaga
     1501 gttagacgag tggagcgcag ccagacaatt tatcgcggtt tagaaggaaa gcggcgctaa
     1561 gacgtcccca atgaaaagtg tgtaaggatg gaaaatgatg tggaaaatcc cttgtgcttt
     1621 cgatcgagct aaccggtgca caggggaatt agcctaaagg aaggtcatct atgaaggcga
     1681 tcgcagtatt tcccggcaaa ccaaacacag tgcatctcac cgagatgccc aaacctgctg
     1741 ttgacgagat tgcgggcggc cgtggtgtgc tcgtcaaagt tctgcgcgtg ggtgtcgatg
     1801 gcacagacaa agagatcaat gccgccgagt atggcaaggc gcccgctggt tacgactttc
     1861 tggtgattgg tcacgagagt tttggccagg ttgaaagcgt tggcccgaat gtaagcgaag
     1921 taaaacccgg cgactatgta gtggcaactg tgcgccggcc cggttcgagt atttacgact
     1981 tgatcggtac caacgacatg accaccgacg atacctattt cgagcggggc atcaatctac
     2041 gccatggcta catgagcgag tactacgtcg atgacgccga gttcatcgtc aagattccgc
     2101 agggattgaa gcacgttgca gtgctactcg aaccaatgac tgtggtggag aagggcattg
     2161 tccaggctta cgagattcag cgtcgcttga aggtatggcg tccgcgccgc gctgcggtgt
     2221 taggcgcagg taccatcgga ctgctcgcga ccctcgtgtt gcgtctgcgc ggtttggaag
     2281 ttacgacttt cggtctgaac cccaagcctt atttgaattc tgacctgatc gaagggatcg
     2341 gatgtcgtta cgagtccacg agggatttgc cactcctgga cggtgctaaa aaatacggac
     2401 cgtttgacat catctttgag gcaaccgggt tctcgccgat ggtcttcgac ggtatgcaag
     2461 cgcttggcaa aaacggcgtg ctggtgttgt cgagcgttac cggaggcgat cgcaagattg
     2521 aggtaccggc tgacaagatc aatttggagt tcgtgctggg caataaagtg atggtgggaa
     2581 cagtcaacgc gaatcgcgac tacttcgagg ccggcgtgaa agatatggcg caagcggaag
     2641 ccgaataccc gggctggctc gggcgtttgc tgactcatcc cgtcaagggt ttggaaaact
     2701 atcaggagct gtttgccaaa ttgagtaatg gcggcggtgc tatcaaagtg ttttgcgaag
     2761 tcgcaccgct atagtcgcac ggacgccgga ttagaaggtc gcatgatcaa agtcaacgag
     2821 caagattgcc gcaatttaga caaggcgctc gagcgtgagt ggttggagac caacggactc
     2881 ggcggctttg cctcagccac gattgccggc gccaacaccc gtcgctatca cgggctcttg
     2941 acggcagcga ccaagccgcc ggtcggtcgg gtcgtcatgc tctccaaatt agaggagacg
     3001 tttgtttccg gagaagtccg ttacgagctt gctaccaacc aataccccgg cgcgacttac
     3061 ccgcagggct ttcagttgtt aacaggcttt cgtctcgatc ctttccccat atcaactttt
     3121 cttgtcgccg ggatcgagat tgagaaaagt gtgtttatgg tcagcggcga gaatacgacc
     3181 gttgtccagt atgatttccg gctcgcgccg tttgaaggag accagcgcgc aacccttccc
     3241 aagtccttgg cttttgagat tcgcccgctg atcgcgtttc gcgattatca cagcaccacg
     3301 catgaaaatg gggcgctgaa cgcgcatgtc gcagtggaac cagggtgcgc ccgggttgcg
     3361 ccttacgctg atcttccggc tctgtatttt gcttatgacg gtggggacat aaatacgaat
     3421 ggcgactggt atcgtaactt cgaatatcta gttgagaagg agcgaggact cgactttcag
     3481 gaagatcttt tcaatccgtt tacacttaga ttctcaattg acccggcaac cttgcgcaaa
     3541 aaatctggcg tgcggctgag cattattgcc tcaaccgaaa ttcgcgactc gaagcgtgcc
     3601 gaagcttatc gcgttgccga gatccaacgc cgcactgaca tcgtgtccgc ggttactcgc
     3661 gggaccgatc ttttgcggac cttaacgctt gctgccgatc aattcatcgt tgcccgtggt
     3721 gaacaaaaga cgattgtcgc cggctaccac tggtttgctg actggggccg cgacacgatg
     3781 attgctcttc caggactaac cttagcgacc ggacgttatg agatcgctaa gagcgtcctc
     3841 ctcgaatttg cccggcacgt ggatcaggga atgttgccta atcgctttcc tgacgcgggc
     3901 gaagagccgg aatacaacac cgtagacgcg acgctctggt tttttgaagc ggtgagggcg
     3961 ctggttcaag cc
//
LOCUS       DASUEX010000592         2290 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_103630, whole genome shotgun sequence.
ACCESSION   DASUEX010000592 DASUEX010000000
VERSION     DASUEX010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103630"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..629
                     /locus_tag="VFV61_09845"
     CDS             <1..629
                     /locus_tag="VFV61_09845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BtpA/SgcQ family protein"
                     /protein_id="HEX4900824.1"
                     /translation="DRPYLKSAVGPEITAAMAVVAAEIRKSIALPLGIQVLAGANSSA
                     VAVALAAGASFVRVEGFVFAHVADEGLIEASAGALLRYRRAIGADHIRVFADIKKKHS
                     AHAITTDVDIVETAKAGEFFAVDGVIISGVATGQPTDPDEVNAVGSAVSVPTIVGSGI
                     TAENIHDYQNADALIVGSSIKQDGLWMNPIDRERARALVRAFETKIEI"
     gene            749..2032
                     /locus_tag="VFV61_09850"
     CDS             749..2032
                     /locus_tag="VFV61_09850"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HEX4900825.1"
                     /translation="MLPLTATSDGMASSKHTLIIGAGPLGLVLFKSLVLGLLLFQTLS
                     FGNAQLSSQPVSSADLQFAPGQSASRIPFKRVGNFIYLHARVNDSEPLWFLLDTGATA
                     SYFDVELAKALGLDKSINNASLGFPGVKLLKQNFQLQPWRLGANNGQVAAGLLGYDFI
                     SRFVVEIDYLRNIVTLHEPNSYKHSGSGEVIPLVLLEDDSGGKVPLVKVRITQPTRAA
                     IEGNFIADTAVRSAISFNTPFVDANKLLDSPQQTIQVPIGAGTMVGESEQPIGRLQKI
                     QLGRFKVNRPIGLFFQDKKGIVASPEFDGVIGNEIWRRFKVIFDYSRQRMILEPNRHF
                     SDFEEYDMTGMLLIAEGKDLKAFRIRRLIENSPATAAGLHEGDIISAVDGKPTSNLTL
                     EGVRRLFKQEGRSYRLTLSRDGQQIQTKIKLRRLI"
     gene            2110..>2290
                     /locus_tag="VFV61_09855"
     CDS             2110..>2290
                     /locus_tag="VFV61_09855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900826.1"
                     /translation="MNSDSTPSSRRPKASKLIPLALFFLLLAAMAIPSAAQTRPRGRD
                     LGIPFPGKTGPMNAIT"
BASE COUNT          537 a          596 c          603 g          554 t
ORIGIN      
        1 acgatcgtcc ttacttgaag agtgctgtag gtccagaaat aaccgcggcg atggctgtcg
       61 ttgccgctga gatccgcaag tcaatcgcgt tgccgctcgg tatccaggtg cttgccggag
      121 ccaattcttc cgcggtggca gttgcgctcg cagccggcgc cagcttcgtg cgggtagaag
      181 gcttcgtctt tgcacacgtt gctgatgagg gtttgatcga agccagcgcc ggcgctttgc
      241 tgcgatatcg acgcgccatc ggagcagatc acattcgcgt cttcgctgac attaagaaga
      301 aacattccgc gcacgccatc acgacagatg tcgacattgt tgaaacggcg aaggctggcg
      361 agttctttgc cgtcgatggc gtaataatta gcggtgtggc aacaggccaa cctacggatc
      421 cggacgaagt gaacgcggtt ggttccgcag tctcagtgcc gacgatcgtg ggctcgggga
      481 ttacggctga gaacattcac gattatcaga atgccgatgc gttaatagtg ggttcttcta
      541 taaagcagga tggcctttgg atgaacccta tcgacaggga acgcgcgcgg gcattggtac
      601 gggcgtttga aacgaagatt gaaatttgaa gaagtctatt gcaggttgcg cgacctgatt
      661 gaggctccgt acagtcgctt acgctgaagg cgtttggtct cgcaacagcc gactaaccta
      721 atgctccata ccctggaaag gattcatgat gcttcctttg actgccactt ctgacgggat
      781 ggccagtagc aaacacacac ttattatcgg tgcgggtccc ctcggcctgg ttctattcaa
      841 gagtctcgtc ctcggcttgt tgctgtttca aactctttcg tttggaaatg ctcaactatc
      901 aagccagccg gtttcttcgg cagatttgca gtttgctcct ggtcaaagtg ccagccgcat
      961 cccgtttaag cgagtcggaa actttattta cttgcacgcg cgcgtcaatg attccgaacc
     1021 gctttggttt ttgctggaca caggagcgac cgcttcctac ttcgatgtgg aactcgctaa
     1081 ggctctcggc cttgataaat ctatcaacaa cgcttcgctt ggctttcccg gcgtgaagct
     1141 actgaagcag aacttccagc tccaaccatg gagactcggc gcaaataacg gtcaagttgc
     1201 tgctggcttg ctcggctatg attttattag ccggtttgtg gttgagatag attaccttcg
     1261 aaacatcgtc actctgcatg agccaaatag ctacaagcat tccggctcag gagaagtcat
     1321 cccgcttgtt ttattggaag atgattccgg cgggaaggta cctttggtga aggtcaggat
     1381 cacgcagccc acccgcgccg cgatcgaagg gaactttatt gctgatacag ctgttcgcag
     1441 cgccatcagc tttaatacgc ctttcgtgga cgcaaacaag ctattggact cccctcagca
     1501 aaccattcag gtacctatcg gcgccggcac catggtgggc gagtcggaac aaccaatcgg
     1561 ccgactgcag aaaattcaac tcggacgatt caaagtcaac cgaccgatcg gcctcttctt
     1621 tcaggataag aaaggaatcg tcgccagtcc ggagtttgat ggggtcattg gcaacgaaat
     1681 ctggcgccga ttcaaagtga tctttgacta ctcgcgccag cgaatgatcc tggaacccaa
     1741 ccgtcacttc tccgacttcg aagaatacga tatgactggg atgctgctga ttgctgaagg
     1801 aaaagatcta aaggctttcc gaatcagacg actgatcgaa aactcaccag caacggcggc
     1861 aggactgcat gaaggcgata ttatttccgc agttgatggc aaacccactt ccaatttgac
     1921 actggaagga gtgcgccgtt tattcaagca ggaaggacgc agttatcgtc taactctttc
     1981 gcgtgatggg cagcaaatac aaactaagat taagttgaga agattgatat agacccttta
     2041 tgacaaagtt tgcgcccggg gtcagctgtg actgttcggg agcggttcgt caacttttgg
     2101 aggattgcaa tgaacagtga cagtacaccc tcgtctcgcc gtccgaaagc gtcaaaactc
     2161 ataccgcttg cgctcttctt cctattactc gcagccatgg caatcccgag tgcagcacaa
     2221 actcggccgc gcggccgcga tttaggaatc cccttcccgg gcaaaactgg tcccatgaat
     2281 gccatcactg
//
LOCUS       DASUEX010000593         2706 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_103641, whole genome shotgun sequence.
ACCESSION   DASUEX010000593 DASUEX010000000
VERSION     DASUEX010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2706
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103641"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..843)
                     /gene="meaB"
                     /locus_tag="VFV61_09860"
     CDS             complement(<1..843)
                     /gene="meaB"
                     /locus_tag="VFV61_09860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HEX4900827.1"
                     /translation="MKPDTDHESTLASLIGRITSGDPRAVARAISKVEDSSDDASALM
                     KEVFPHSGKGLVIGITGAPGAGKSSLVDKLALLYRTQNERVGIVAVDPSSPFSGGAIL
                     GDRIRMQALGLDKGVFIRSMATRGNLGGLARATVDAVSILAAAGYEKVVVETVGVGQD
                     EVEIVKAADVCVVVLVPGMGDDIQAIKAGIMEIADIFVINKADREGVYSTEKELEALL
                     SLAHREDGWQPPIVKTVATENKGIADLAAAIERYKDFHFSSDLGKQHRRAVARWRILE
                     LLREK"
     gene            complement(860..2008)
                     /locus_tag="VFV61_09865"
     CDS             complement(860..2008)
                     /locus_tag="VFV61_09865"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX4900828.1"
                     /translation="MEFELSEEQQQIKMSVREFAEAEIAPHVMEWDEAQHFPIELKPK
                     LAELGLMGVIFPEKYGGAGMGYVEYATIIDELSRVDGSVGISVAAHNSLCSNHIYEFG
                     NEEQRQKYLVPLARGEHLGAWGLTEPSAGSDASGTRSTAVRNGDGWIVNGSKNFITHA
                     INADTCVAVAVTDRAQKSRGITAFIFEKGMPGFSPSKKENKLGLRASETASVVFEDCH
                     VPDANRLGEEGLGFVNAMQILDGGRISIAALAVGIAQGAYESALRYSKERQQFGKPIS
                     EFQAIQFKLANMATQIDAARLLMYRAAWLKDQKRKVTKESSMAKLFASEMSVNVCEEA
                     IQIHGGYGYTKDYPAEKYWRDSKLCTIGEGTSEIQRLIIAREILKQAG"
     gene            complement(2156..>2706)
                     /locus_tag="VFV61_09870"
     CDS             complement(2156..>2706)
                     /locus_tag="VFV61_09870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEX4900829.1"
                     /translation="KGYLSSVSDPEGRPLVTELLPASLGRLYPVGRLDFNTEGLLLLT
                     NDGDFTNFITSARNRVEKVYQAKVKGLPPEAALQRIRRGVILDDGTRTAPARITKVDE
                     TENNAWFEIVLHQGKNQQIRRMFDLIGHSVVKLKRARIGFLYDERLRPGEWRRLSPQE
                     VQRFMKGPRVKKTSPTVARRAR"
BASE COUNT          600 a          822 c          668 g          616 t
ORIGIN      
        1 cttttcccgc aacagttcca agatcctcca gcgcgcgacg gcacggcggt gttgcttgcc
       61 caaatcggaa ctgaagtgga aatccttata acgttcaatc gcagccgcca gatcagcaat
      121 gcccttgttc tccgtcgcaa ctgtcttgac gattggtggc tgccagccgt cttcgcgatg
      181 ggccagcgaa agcagagcct ccaactcttt ttccgtggag taaacacctt cgcgatccgc
      241 tttgttaatc acaaatatat cggcaatctc catgatgccg gccttaatgg cttgaatgtc
      301 atcgcccatc cctggaacga gcacgacgac gcagacatcc gcggccttta cgatctcgac
      361 ctcgtcctga ccaaccccga ccgtttccac gacgaccttc tcgtagccgg ccgcggcgag
      421 gattgaaacg gcatccaccg tggcacgtgc caaaccgccg agatttcctc gtgtcgccat
      481 tgaacggata aagacaccct tgtccaggcc gagtgcctgc atcctgatgc gatcacctaa
      541 gatggcaccg cccgagaaag gactggaagg atcgaccgcg actatcccaa cgcgttcgtt
      601 ctgcgtgcga tagagcaaag ccagcttatc aaccagggaa gacttaccgg cgccaggagc
      661 gcccgtgatt ccgattacca aacctttgcc actgtgtgga aagacttcct tcatcagagc
      721 cgaagcatca tcggaactat cttcaacttt tgagattgcc ctagccaccg ctcgcggatc
      781 gcccgaagtg atgcggccga tcaaggaagc cagggtcgat tcgtgatcgg tatcaggttt
      841 cattcgctgg ctcgccggat caacctgctt gcttcaaaat ctcgcgggca ataatcaaac
      901 gctgaatttc agaagtgccc tcgccaatcg tgcagagctt ggaatcgcgc cagtacttct
      961 cggccggata gtccttcgta taaccgtaac caccgtgaat ctgaattgcc tcttcgcaaa
     1021 cattgacgct catttcggag gcaaacaact ttgccattga agactccttg gtaaccttgc
     1081 gtttctggtc cttcaaccag gcggctcgat acatcaagag gcgcgccgca tcgatctgcg
     1141 tcgccatatt tgcgagcttg aactgaatgg cctggaattc cgaaatcggc ttaccaaact
     1201 gctggcgctc tttcgaatat cgcagcgctg actcgtaagc accctgggcg ataccaaccg
     1261 ccagcgccgc aatcgaaatg cgtcccccat ccagaatctg catcgcgttg acaaagccca
     1321 ggccctcttc tcccaagcgg tttgcgtccg gaacatggca gtcttcaaag accacgctcg
     1381 cggtttctga ggctcgcagg cccagcttgt tttctttctt ggacggcgag aaccccggca
     1441 ttcctttttc gaaaataaaa gcagttatcc cacgactttt ctgcgcccga tctgtcaccg
     1501 cgacagcgac gcaggtgtct gcgttaattg cgtgcgtgat aaagtttttc gagccattaa
     1561 cgatccagcc atctccatta cggaccgcag tcgagcgtgt gccggaggca tcggagcccg
     1621 cactgggttc ggtcaatccc caggcgccga ggtgttctcc gcgcgcgaga ggcaccaggt
     1681 atttttgcct ctgctcctcg ttgccaaatt cataaatgtg gtttgagcaa agggagttgt
     1741 gcgcggccac agaaatgccg acggaaccat caacccggga aagctcatca atgatcgtcg
     1801 cgtattcgac ataacccatc ccggcgccgc catacttctc cggaaagatc acacccatca
     1861 agccgagttc agcgagtttt ggtttcaact cgatgggaaa gtgctgcgct tcgtcccatt
     1921 ccatcacgtg gggtgcgatc tccgcctcag cgaactcgcg cacgctcatc ttgatctgtt
     1981 gttgttcttc gcttagctca aattccatcg cttccccatt gactcaagtc ccgacacaac
     2041 aaaggcatcc ttgccgcaag ataacaggca agaatgcctg ccggaaaact ctaacgcttt
     2101 gccggttagt aattctaccc cgccgtcgat aagaactgta gctgagagcc gccgcttaac
     2161 gggcccgccg ggcgacggtt ggcgaggtct tcttaacacg tgggcccttc atgaagcgct
     2221 gcacttcctg tggcgagagc ctgcgccact cacccggccg taagcgttcg tcgtaaagaa
     2281 aaccaatccg cgcccgtttc aatttcacaa cggaatgacc gatcagatcg aacattcggc
     2341 gaatctgctg gttcttcccc tgatgcagaa cgatctcaaa ccaggcgttg ttttcggttt
     2401 cgtctacctt tgtaatcctg gcgggcgcag tgcgcgtgcc gtcgtcgaga attacgccac
     2461 gccggattct ttgcagcgct gcctcgggtg gcaatccttt cactttcgcc tgatagactt
     2521 tttccacgcg attgcgcgcc gacgtaataa agtttgtgaa atctccgtca ttcgttagca
     2581 acagcaatcc ttccgtgttg aagtccaggc gaccgaccgg atagaggcgg cccagggagg
     2641 ccggcaagag ttcagtaact aaaggcctcc cctccggatc agaaacactc gataaataac
     2701 ctttgg
//
LOCUS       DASUEX010000594         2052 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_788053, whole genome shotgun sequence.
ACCESSION   DASUEX010000594 DASUEX010000000
VERSION     DASUEX010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_788053"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1145)
                     /gene="yidC"
                     /locus_tag="VFV61_09875"
     CDS             complement(<1..1145)
                     /gene="yidC"
                     /locus_tag="VFV61_09875"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEX4900830.1"
                     /translation="MQQKRLLIALILSSAILFGWSYLYPPGPPANQKQGATPSPTASP
                     SATQTVTSNNQTAPAAPAPAPNVSAAPQRMITVRTPLYDAKFDTLGAEPVSWILKLNK
                     NNNTPIYSVGANKSQKQPLELISAEGLKRQPRMVPFQLQTGDATFDSVLSSSTYKVEG
                     VDQASGDVDVNVKSGEKKELTFVYDDGNGNQVRKKIVFDADQYETDLSLLVKRGDQTV
                     PSVKLAIGPSIGDQGVPHHTFYSVAPEAVAFVNSKVERHPPAGINGNSSSPDRLTLNG
                     PVDWAAVADTYFAMVAVPVNKPEGLEYRTVAYEHKPNGGSAEKRFLVTAFLPVPADGS
                     RTVIYAGPKDHDLLTNGSASVTGLVGRNIDLEGLIDYGFLGWLSRPLA"
     gene            complement(1146..1355)
                     /gene="yidD"
                     /locus_tag="VFV61_09880"
     CDS             complement(1146..1355)
                     /gene="yidD"
                     /locus_tag="VFV61_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002474682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEX4900831.1"
                     /translation="MKSVLISLLRFYKLAISPLLPPSCRFIPTCSEYAVDAISRYGAL
                     RGSGMAVRRLLRCHPFHPGGYDPVK"
     gene            complement(1365..1724)
                     /gene="rnpA"
                     /locus_tag="VFV61_09885"
     CDS             complement(1365..1724)
                     /gene="rnpA"
                     /locus_tag="VFV61_09885"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEX4900832.1"
                     /translation="MRKYLRDTSEFQHVYRNGKRYEGVFITAFVIENQELNHRLGVTA
                     SKKALGKAVQRNRAKRLLREAFRSNETFLSELARNYDWVLNAKAAVLHEKLDAPFQDL
                     KGIIEKVGRLEARNTVR"
     gene            complement(1741..1887)
                     /gene="rpmH"
                     /locus_tag="VFV61_09890"
     CDS             complement(1741..1887)
                     /gene="rpmH"
                     /locus_tag="VFV61_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006404565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEX4900833.1"
                     /translation="MSKRTFQPNNRRRAKTHGFRARMASKNGRLVLQRRRAKGRKRLT
                     PAHY"
BASE COUNT          462 a          560 c          530 g          500 t
ORIGIN      
        1 gcaagcggtc gggaaagcca gccgagaaaa ccgtagtcga tcaatccctc gagatcgatg
       61 tttcgcccaa caagcccggt gacactcgca ctgccattcg tcagcagatc gtgatctttc
      121 ggacccgcgt agataacggt gcgggagcca tccgcgggaa cgggaagaaa cgctgtgact
      181 aaaaaccgct tttccgcact accaccgttt ggcttgtgct cgtaagccac agtccggtat
      241 tcgagccctt ctggtttatt gacgggcacc gcaaccattg cgaaatacgt gtctgcgact
      301 gccgcccaat caaccgggcc gtttagcgtg agtcgatccg gactgctgct gttgccattg
      361 attcccgccg gcggatgacg ttccacctta ctattaacga aagcgacggc ctccggcgct
      421 accgagtaga acgtgtgatg cggcacgccc tgatcgccga tactcggacc gatcgcgagc
      481 ttcactgaag gcaccgtctg atcgccacgc ttaaccaata gggaaagatc ggtttcatac
      541 tggtcggcat cgaaaacgat cttctttcgt acctgattcc cgttgccgtc gtcgtaaacg
      601 aacgtgagtt cctttttctc acccgacttg acgtttacgt cgacgtcgcc actcgcctga
      661 tcgacacctt caactttgta tgtagacgag ctgagaacag aatcaaatgt cgcatcgcca
      721 gtctgcaact ggaacggcac catgcgcggt tgtcgcttca atccctcagc agagatcagc
      781 tcaagcggct gcttttgact cttattagcg cccaccgagt agatcggtgt gttgttgttt
      841 ttattgagct ttaagatcca actgactggc tcagcgccga gcgtgtcgaa cttcgcatcg
      901 tacagcggtg tccggaccgt gatcattcgt tgcggcgccg cgctcacatt cggggctgga
      961 gcaggagccg ctggagctgt ctgattgttg gaagtaacag tttgtgtcgc tgatggactg
     1021 gctgttggag acggcgtcgc cccctgcttc tgatttgcag gcgggcctgg cggatagaga
     1081 taactccacc caaacagaat tgctgatgat aaaattagag ctattaaaag tcgtttctgt
     1141 tgcatctatt taactggatc gtatcctccc ggatggaacg ggtgacaacg gagcagtcgc
     1201 cgcacagcca tgccacttcc gcgcaaagca ccgtaacgag agatggcatc aacggcatat
     1261 tcggaacatg tgggaatgaa tctgcacgaa gggggtagaa gcggggaaat cgccaactta
     1321 taaaatctga gtaatgagat cagaacgctc ttcacaaccg gcgcttatct aacagtattg
     1381 cgggcttcca accgtccaac cttttcgatt atccccttca gatcctggaa aggcgcgtca
     1441 agcttttcat gcaataccgc tgccttcgca ttcaagaccc aatcgtaatt tcttgcgagc
     1501 tcgctcaaga acgtttcgtt tgaacggaac gcctctcgaa gcaaacgctt cgctcgattc
     1561 cgctgcaccg ccttcccgag tgctttcttt gaagccgtca ctccgaggcg atgattcagt
     1621 tcctggtttt caatgacaaa cgcggtgatg aatacgcctt cataccgctt gccgttccgg
     1681 tagacgtgct ggaattctga agtatcccgc aagtacttcc gcatgcatca agcggcaatc
     1741 tcagtaatga gccggggtca gccgttttct tcctttggcg cgacgtctct ggaggacgag
     1801 gcgtccgttc ttgctcgcca tgcgagcgcg gaagccatgc gtctttgcgc gtcgtctgtt
     1861 gttcggctga aaggtgcgct tcgacataga aatgaattct ccttaaaaac gaaaacccta
     1921 agactagccg cgccttgtgt ttatgtcaag gaaagtggcc gcggattaca ctgattaaat
     1981 atcgatcagc gccggtcaga aaattatccg tgaatccgtg gttaatgttt gctgttcaac
     2041 aatgtcgtgt ac
//
LOCUS       DASUEX010000595         2873 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1016304, whole genome shotgun sequence.
ACCESSION   DASUEX010000595 DASUEX010000000
VERSION     DASUEX010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2873
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1016304"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="VFV61_09895"
     CDS             <1..499
                     /locus_tag="VFV61_09895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900834.1"
                     /translation="TFTNNDGFVQRVRQSIPPVHSSRPDWIITAQLAKELGVDFEFQM
                     SATSVFREIAATIPAYAELRYPLLKDETHPVQVKHPEAPPRDLSAEVEQLKQLVSELP
                     EETNKQTVTPDVGHELFKPGTLTGRVPQFPLLASGNPRPETLRISPLYQIKGIADLRV
                     PIADS"
     gene            699..1730
                     /gene="nuoH"
                     /locus_tag="VFV61_09900"
     CDS             699..1730
                     /gene="nuoH"
                     /locus_tag="VFV61_09900"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEX4900835.1"
                     /translation="MDWTNPLIFSVAKIVAITVAFPAVMLLVALTVLAERRVLAFIQG
                     RVGPNRVGYFGVLQPFADFIKTMVKEDLVPDKSTKFVFLLAPMIAVVTAIMAILVYPF
                     GPTITLPFLGPIQLVIARFDVALLYVLGVTSVGVYGIALAGWSSNNKYSLMGGLRSSA
                     QMISYELSLGMSLIGVILLTGTLDIYSIVEQQSGWYGMNWNILYQPLGFVLYLISAIA
                     ETNRVPFDLPEAETELVAGFHTEYSSIKFLLFFNAEYINMITVSMLATTLFLGGWNGP
                     FVEQLPILGVAYFAIKILFFLFLYIWIRGTLPRFRFDQLMDFGWKVLIPLAILNILIT
                     ATVLFVRGS"
     gene            1743..2408
                     /locus_tag="VFV61_09905"
     CDS             1743..2408
                     /locus_tag="VFV61_09905"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEX4900836.1"
                     /translation="MITTLLFILFAGTAIGCAIAMVAQRNPLYSAISLIGVLLSLACL
                     YVMLAAPFIAAVQVIVYAGAIMVLVIFVIMLLNVEDEEPRRNRLAFLAPTAIGLAAVL
                     IAEAAFILYSVQASPYTPRENVSNVGLTSSVGAGLFTEYLLPFEITSVLLLMAIVGAM
                     SLARRSNILSATSRVVPGSQVLAMNVLDSLATDETAVETDSTVEARFLMGVDRPSDPD
                     KQG"
     gene            2413..2718
                     /gene="nuoK"
                     /locus_tag="VFV61_09910"
     CDS             2413..2718
                     /gene="nuoK"
                     /locus_tag="VFV61_09910"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002638444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEX4900837.1"
                     /translation="MGPQLSWYLALSAILFTIGVVGVIIRRNVIAMFMCIELMLNAVN
                     LTFVAFSSFFQDVTGQLFVFIVMTVAAAEAAVGLGIIIAIFRNRETLDVDEASILKL"
BASE COUNT          564 a          755 c          767 g          787 t
ORIGIN      
        1 gaccttcact aacaacgacg gttttgtgca gcgggtacgg cagtcgattc ctccggtgca
       61 cagctcccgg ccagactgga tcatcaccgc tcagcttgcg aaggagctgg gcgttgactt
      121 cgaatttcag atgtctgcga cttcggtgtt ccgcgagatc gcggcgacca ttccggcata
      181 tgccgaactt cgttacccct tgctgaagga cgaaacacat ccggtgcagg tcaaacatcc
      241 ggaggcgccg ccgcgagatc tctcggccga ggtggagcaa ctcaaacaat tggttagcga
      301 gctacctgaa gaaaccaaca agcaaacagt cactccggac gtgggtcatg aattattcaa
      361 gccggggacg ttgaccggcc gagtgccgca gtttcctttg ctcgcgagtg gcaatccgcg
      421 gcctgagacg ttgagaattt cccccttgta tcagatcaaa ggcattgccg atttacgagt
      481 gcccattgcc gattcatgac attgccgatt gccaattggc aattgccgat tttgtgctgg
      541 gagcgttgta tctgatctgc gcggtctgtg atgcggctaa gccttatttg cttcttgaga
      601 cttatttttc aaggcggagc tcaaatgtga tcggcaatcg ccaattggca atcggcaatg
      661 tggataatcg ccaatgtgga taatcggcaa ttaactacgt ggactggact aacccgctaa
      721 tcttttccgt cgccaaaatc gtggcgatta ctgtcgcgtt tccggcggtc atgttgctcg
      781 tggcgcttac ggtgcttgcc gagcgacgcg tcttggcctt cattcaggga cgggttggtc
      841 cgaatcgcgt cggttatttt ggggtgctgc aacccttcgc ggatttcatc aaaacgatgg
      901 tcaaggaaga cctcgttcct gataagtcga cgaagtttgt ctttctcctg gcgccgatga
      961 tcgctgtggt cacggccatc atggcgattc tggtttaccc cttcggcccc acgatcacgc
     1021 ttccctttct gggacccatt caactcgtca tcgcccgctt cgatgtagca ttgctctatg
     1081 tgttgggggt cacttcggtg ggggtctacg gcatcgcgct cgcgggctgg tcttcaaata
     1141 acaaatacag cttgatgggc ggcctgcgct cgtcggccca gatgatctct tatgaacttt
     1201 ccctcgggat gtccctcatc ggagtcattc tgctcacggg tacgctggat atttacagca
     1261 tcgtcgaaca gcaatctggt tggtacggca tgaattggaa cattctttat caacctctgg
     1321 gattcgtgct ctacctgatc tcggccatcg ctgaaaccaa ccgcgtgcct tttgatttgc
     1381 cggaagctga gacggaactt gtggccgggt ttcacaccga gtacagctcg atcaagtttc
     1441 tcctcttctt taatgccgaa tacatcaaca tgatcacggt ttcgatgctc gccactacct
     1501 tgtttctggg tggctggaac ggaccgttcg tcgagcaatt accgatcctt ggcgttgctt
     1561 attttgcaat caagatcctg ttcttcctgt ttctctacat ttggatacgc ggcacgctcc
     1621 cgcgctttcg ctttgaccag ttgatggact ttgggtggaa ggttttgatt cccctggcga
     1681 tcctcaacat attaataacg gcgacggttt tgtttgtgcg cggcagttaa cgcacgctga
     1741 aaatgataac gactctgctc ttcatattat ttgcgggaac cgctatcgga tgcgcgattg
     1801 ccatggtggc gcagcgtaat ccgctttaca gcgcgatctc gctgattggc gtgctgctgt
     1861 cactggcctg tctttacgtg atgctggcgg cgccgtttat tgcggcagtt caggtaatcg
     1921 tctacgccgg cgcgatcatg gtgcttgtca tattcgtgat catgttgctc aacgtggaag
     1981 atgaagaacc gcgccgcaat cgcctcgcct tcctggcgcc gacagcaatt ggtctagctg
     2041 ctgtcttaat cgccgaagcg gctttcatcc tttactcagt ccaggcgtct ccctacacac
     2101 cgcgagaaaa tgtttcgaat gttggtttga cgtcatccgt gggcgccggc ctgttcacgg
     2161 aatatctgct gccgtttgaa atcacttcgg tcttattgct gatggcaata gtgggcgcaa
     2221 tgtccctcgc caggcgcagc aacattctct ctgccacctc tcgcgtagtt cctggttcgc
     2281 aggtgttggc aatgaacgtc ctggattcgc tggcgacaga tgagacggcg gtggaaaccg
     2341 attcaactgt agaagcgcgc ttcttgatgg gtgtcgatcg cccgagcgat ccagacaaac
     2401 aaggttagat atatgggtcc gcaactttcc tggtatctcg cgctctctgc cattctgttt
     2461 acgattggcg ttgtcggcgt gatcatccgc agaaatgtaa tcgcgatgtt catgtgcatc
     2521 gagctgatgc tgaacgccgt gaatctgacc ttcgtcgcgt tctcaagttt cttccaggac
     2581 gttacgggtc agctttttgt tttcatcgtg atgacggtcg cggccgcgga agcagccgtg
     2641 ggattgggaa tcattatcgc tatcttccgc aaccgcgaaa cattggatgt ggatgaggct
     2701 agtattttga aattgtaaaa atggcttttg gtctttggtc tttggttttg gtttttgttg
     2761 ctcccacgtg ggtgctagtc aaagacccaa aaccaaagac ccaagacccc ggtttacatg
     2821 gataacaacc tcttaagtct gatcatcttc gctccgctgg cgggcgcggc gat
//
LOCUS       DASUEX010000596         3762 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1016474, whole genome shotgun sequence.
ACCESSION   DASUEX010000596 DASUEX010000000
VERSION     DASUEX010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3762
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1016474"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1462)
                     /locus_tag="VFV61_09915"
     CDS             complement(<1..1462)
                     /locus_tag="VFV61_09915"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX4900838.1"
                     /translation="MKYCPSCKTKYEDTVGFCPVHGEVLEDDLSSIVGTVLDGQYQIE
                     ALLGKGGMGAVYRARHILLGDRVAIKILPPEVRTNAEWLRRFQREGQAARRFRHPNAV
                     TVYDLRTNADGTVYMVMEYVEGHTLGDEIKQRGRLTPPEVVELLEPIMSVLNTAHAMG
                     VVHRDLKPDNIMIGKSNDGERVIKLLDLGIAKMREIAGGDGGGNTALTMAGQVLGTPH
                     YMSPEQWGEIPRDGDSEIDGRADIYSLGIVCYEMIVGKRPYSGTTLFELRREHVSVIP
                     PPVYETVPGVPRAFGDAITQATAKDRADRFATAGDFAAHLRAAVNSSDAAFGLDRTIP
                     DIPRAIESEAHTSGISAGSTNADVDAPTILTLDSPLTTPHDRASHTAPQVPGAPAKAA
                     GIAASFPAEPPSIVTIPQQRPKVAVPFPQAQPQAKSAGGSRGLILAFVGLVVVLALGV
                     GGFFVVKKLTTSSESGGSITSGNNGAGTSGTATTKEV"
     gene            1651..1938
                     /locus_tag="VFV61_09920"
     CDS             1651..1938
                     /locus_tag="VFV61_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900839.1"
                     /translation="MIWIVRALLAILFIVGVMRLILFFRSGAPPFIEPGQPVGKQRLL
                     MLGFLNILISGFSFFLLQTNSAEPWEILFGGPLIVLVFEVTFRKDSILPFQ"
     gene            complement(1977..>3762)
                     /gene="glgP"
                     /locus_tag="VFV61_09925"
     CDS             complement(1977..>3762)
                     /gene="glgP"
                     /locus_tag="VFV61_09925"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HEX4900840.1"
                     /translation="HNSLPLYSGGLGMLAGDHLKSASDIGIPLVAVGLLYRYGYFTQR
                     FRRDGWQEEHYGDTHPQELPLQQVKDDDGTPLTIEVLIRKRMVRAQVWRAAVGHVQLY
                     LLDTNIPENSEIDRLVTGHLYGGDRETRIVQEMLLGIGGIRLLRRLKLEPSVYHLNEG
                     HSAFLTLELAREFINSKNISFQEATKLVRELCVFTTHTPVAAGNDEFEPALIRKCFGS
                     DYWKELRLSDDEFFDLGRVDPANDLENYGLTPLAIRMCRSTNGVSKKHGEVSRELWQK
                     LWPSKQLADIPITHITNGVHAPTWVAPLIRTLYEEYAGEDWTTVAHDRTRWEESIARL
                     SDQELWTAHSLLKQRLIAFIRERSFQARQERGESSEYTESARTMFDPNALTIGFARRI
                     AAYKRWSLLLTDPDRLQGLMTDPDRPVQFVFAGKAHPQDQGAKLILQQLALWKYNSHV
                     QNRAVFIQDYDQEIARQLVQSVDVWLNVPRRPLEASGTSGEKVAMNGGLNLSVLDGWW
                     MEGYDGTNGFAVGGTSVDGAPELVDLADAESLYRVLEEEVIPTYYERDQSELPRKWIA
                     MMKRSIVTLVPEFNSDRMVAEYARKIYK"
BASE COUNT          808 a         1089 c         1007 g          858 t
ORIGIN      
        1 ctacttcctt cgtagtcgcc gttcctgagg tccccgctcc gttgtttccg gacgtgatgc
       61 tcccgccact ttcactgctg gtggttagtt tcttaaccac gaagaacccg cctacgccca
      121 gagccagcac caccaccaga ccgacaaatg ccagaatcaa acctcgacta cctccggcgg
      181 atttcgcttg cggctgcgcc tgaggaaacg gaactgcaac cttgggacgc tgctgcggaa
      241 tggtgacgat cgagggcggc tcggcgggaa agctggcagc tataccggcc gctttcgccg
      301 gcgcgccagg cacttgtgga gccgtatgcg aggcgcgatc gtgaggcgtg gtcaaaggcg
      361 aatctaaagt cagaatcgtc ggcgcatcca cgtccgcgtt ggtgctgcct gccgaaatgc
      421 cggatgtgtg cgcctcagat tcaatcgctc gcggtatgtc cgggatggtc cgatcaagac
      481 cgaaagcagc atcgctggag ttcacagccg cacgtaaatg agcggcaaaa tcaccggcag
      541 tagcaaagcg atcggccctg tccttagccg tggcttgggt gattgcatcg ccaaaagcgc
      601 gcggcacacc cggcaccgtc tcgtagaccg gaggtggaat aactgagacg tgttcacgtc
      661 gtaattcgaa tagcgttgtt ccgctgtaag gtcgcttgcc cacaatcatc tcgtagcaaa
      721 cgatgccgag actgtagata tcggcgcgac catcgatctc ggaatctccg tcgcgcggta
      781 tttcgcccca ctgctcgggc gacatgtagt gcggcgttcc caacacctga cccgccatcg
      841 tcagcgccgt attcccgccg ccatcacctc cggcaatttc ccgcatctta gcgatgccca
      901 aatcaagcag cttgattacc cgctcgccgt cgttcgactt cccgatcatg atgttgtcgg
      961 gtttcagatc acgatgaacg actcccatcg cgtgcgcagt gttgagcacg ctcataatgg
     1021 gctccagcag ctcgacaacc tccgggggag ttagacgccc tcgttgcttg atctcatctc
     1081 ccagggtgtg gccttcgacg tattccatca ccatgtagac agtgccgtcg gcgttcgtgc
     1141 gcaggtcata gacagtgact gcattcgggt ggcgaaaacg gcgcgcagct tgtccttcgc
     1201 gctggaagcg tcgcaaccac tctgcgttgg tgcgcacctc aggtggcaaa atcttgatgg
     1261 cgacgcgatc gcccaggagg atgtggcggg cgcgataaac cgcgcccatt ccgcctttgc
     1321 ccaacaacgc ttcaatctga tactggccat caagcacagt accgacgatc gatgacaggt
     1381 cgtcttccaa cacctcgccg tgaacgggac aaaagccgac agtgtcttcg tatttagttt
     1441 tgcagcttgg gcagtacttc atgaaaggat gtactcagaa caacgggcgg ggttcgcggt
     1501 aaggtctgca atcctatgcg aaatccgaac cgggctgaac gaacggtagt ctaacacagg
     1561 gaacgggcag ataattactg ttagggaatt gtggtaccgc cgtcagtctc gaattccgtt
     1621 gcgggtagat gcattggagg ggaacttgcc gtgatttgga tcgttcgcgc cctgctggca
     1681 attctcttca tcgttggagt catgaggctg atcctgtttt tccggagcgg agctccacct
     1741 tttattgagc caggtcagcc tgtgggaaag caacgactgc tgatgctcgg gtttcttaat
     1801 atccttatct ctggattcag tttcttccta ctgcaaacca acagcgctga accttgggaa
     1861 attctctttg gtggtccact gatcgttctg gtttttgagg tcacctttcg aaaggactcg
     1921 atccttccct ttcaataaaa accgaaacag ctcttactga atttcccgca tttacgttac
     1981 ttgtaaatct tccgagcgta ctccgcgacc atgcgatcgc tgttgaattc cggaacgagg
     2041 gtgacgatcg atcgcttcat catcgcaatc catttgcgcg gcagctcgct ttggtctcgt
     2101 tcatagtagg tgggaatcac ttcctcttcg aggacccgat aaagcgactc ggcatccgct
     2161 aaatcaacaa gttcgggcgc cccgtcgact gacgtgccgc ccacggcgaa accatttgtg
     2221 ccgtcgtatc cctccatcca ccagccatca aggaccgaga gattcaggcc accattcatc
     2281 gcgacctttt cgccgctcgt acccgaagct tccaaaggcc ggcgcggaac gttcaaccaa
     2341 acatcgactg actgcaccag ttgccgcgcg atctcctggt cgtagtcctg aataaacacg
     2401 gcccggtttt gaacgtggga attgtatttc cagagggcga gttgttgcag gatgagtttt
     2461 gcaccttgat cctgaggatg agccttgccg gcgaacacga actgaaccgg acggtctggg
     2521 tcggtcatta atccctgcag ccggtccgga tccgtaagca gtaagctcca gcgtttataa
     2581 gccgctatgc gtcgcgcaaa tccaatcgtc aacgcattcg gatcaaacat ggtgcgtgcg
     2641 gattcagtgt attcactgct ctcgccgcgc tcctggcgcg cctgaaagga gcgctcgcga
     2701 atgaaagcga tcagtcgctg tttcagcaac gaatgcgccg tccacagctc ctgatcagaa
     2761 agccgggcga tactctcttc ccaacgtgtc cgatcatgcg cgaccgtagt ccagtcttcc
     2821 ccagcgtatt cctcgtaaag ggtccgtatt agcggcgcta cccaggtggg agcgtgcaca
     2881 ccgttggtga tgtgggtgat gggaatgtcc gcaagctgct ttgacggcca taacttctgc
     2941 cagagttcac gcgaaacttc tccgtgcttc ttactgaccc catttgtcga acgacacatg
     3001 cgaattgcca gtggcgtgag gccgtagttc tccaggtcat tcgcaggatc aactcgccct
     3061 aaatcgaaga actcgtcgtc gcttagtctc agttccttcc agtagtcaga gccaaagcat
     3121 ttccgtatca aggccggttc gaactcgtcg tttccagcag ctactggagt gtgcgtggta
     3181 aacacgcaga gctcgcgaac gagtttcgtg gcctcctgga aactaatgtt tttggaattg
     3241 atgaactcgc gggccagttc cagagttaaa aaagcggagt gaccttcgtt aaggtgatag
     3301 acgctcggtt ccagcttcag ccggcgcagt aatcgaatgc caccgatgcc gagcagcatt
     3361 tcctgcacaa ttcgggtttc gcgatccccg ccgtaaaggt gaccggtaac caggcgatca
     3421 atctccgaat tctccggaat gttcgtatcc agcaaataga gttgcacgtg gccgactgcc
     3481 gcacgccaga cttgcgcgcg caccattcgc ttacggatca gtacttcaat cgtgagtggc
     3541 gttccgtcgt catctttgac ctgctgcagc ggtaactcct gcgggtgcgt atcaccatag
     3601 tgctcttcct gccaaccgtc acgccgaaaa cgctgcgtaa aatagccgta gcgataaagc
     3661 aaaccgacag caacgagcgg aatgccaatg tcgcttgctg atttcaaatg atcaccggcc
     3721 agcatgccca ggccacctga ataaagaggc agggagttat gc
//
LOCUS       DASUEX010000597         3931 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_788689, whole genome shotgun sequence.
ACCESSION   DASUEX010000597 DASUEX010000000
VERSION     DASUEX010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3931
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_788689"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1591)
                     /locus_tag="VFV61_09930"
     CDS             complement(<1..1591)
                     /locus_tag="VFV61_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900841.1"
                     /translation="MRLATRALFTALVMLVLCAAVSAQTLRKENDPRNQAPTVGTGGP
                     PGGPTGLFTIYDGDTIRRGEYTFSIAYSNYDRDPGDVDIVETPLSFNIGLNDYIELFF
                     KTNGYRGVKVNNPRNLSGFYLPNSQLLFSASLRCSPQAIILAPERVSGTQNITGTVFR
                     PAGPPCNVGGQRFVQFPFVGGVGPNFGLTGNAIAPPFVSRLGVPTGGGGNYGDAAFFP
                     GYGSPVGSILPGVVLTTRTIPANLTFAAQVVPDLYTIAPTYIADAPFINRLYGESMFG
                     DFTVGGKIRFTGPNNPLGVGLVAFYRFYPDKADDFSGFNQLQRGASPGGDIGDFGAVG
                     FVSGRLAKHVNLSANVGFILNSNPRSEAFGGRNVSLLNRPDELVAGVGFDFPVNKHFQ
                     IITEVKSIQYFRGRTENAFGNNPVDILGGVRIFPRRWLGFSAAYRQHVNQQGDRLFGL
                     FDHTYPAGFRESEDPHGFLAQFFIGHRNPRGLPIPPNQPPTVSVSSSSSSVTLPCPEG
                     TSSAVCTPSASREVTLTADSRD"
     gene            complement(1868..3151)
                     /locus_tag="VFV61_09935"
     CDS             complement(1868..3151)
                     /locus_tag="VFV61_09935"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEX4900842.1"
                     /translation="MPNAVYVDDIREIKEANPFESMMSRFDRAAKILNLDPDLYAVMR
                     VPNRELKVYIPTRMDSGRIQVFEGYRVQHNFARGPAKGGIRYAPDVNIDEVRALAAWM
                     TWKCAVVNVPFGGAKGGVVCDPQQMSMGELERMTRRYASELLDFIGPEKDVPAPDMNT
                     NEQTMAWIMDTYSMHARHTVTAVVTGKPIDLGGSSGRREATGRGILFVINEAIKRFQM
                     TPTTTRVVVQGSGNVGGIGARLLHEAGYQVVAISDVHGGIYNPKGIDIPAALDYLQTT
                     RSFEGYEGVETVSNSELLELECDVLVPAATENQITSQNAHRIKCKVLAEGANGPTTAA
                     ADEVLHQNGVFVVPDILANAGGVTVSYFEWVQDRMGYFWREDVVNQRLQDTMVASFND
                     LCRYATAHHVDTRTAAYMLAIDRVAYDTRMRGIYA"
     gene            complement(3179..>3931)
                     /gene="larE"
                     /locus_tag="VFV61_09940"
     CDS             complement(3179..>3931)
                     /gene="larE"
                     /locus_tag="VFV61_09940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HEX4900843.1"
                     /translation="VAFSGGVDSTYVAYVANSELGERALCVTGESASLPSYQRDETAR
                     VAGQFGFRVETIQTQELENAEYVTNGPNRCFFCKDELYQKLEPFARERQIQFVLDGST
                     TDDLLDYRPGRVAAGQHGVRSPLVEVGLSKLEVRELSRRAGLPTWDKPASPCLSSRIA
                     YGTHVTIERLTTVDQGEDILRSLGFREFRVRHHDQIVRLEIAPAEMDRALRREMVDEL
                     ARRFRELGFKYVTLDLHGYRSGAMNEILAQGI"
BASE COUNT          936 a         1112 c         1038 g          845 t
ORIGIN      
        1 gatcgcgcga gtcggccgtc aaggtaactt cgcggctcgc gctaggagta cagactgccg
       61 agcttgtgcc ttccgggcac ggcagcgtga ccgacgagct cgaggaagat actgacactg
      121 tcggcggttg gttcggcgga attggcaatc cacggggatt cctatgtccg ataaagaact
      181 gcgccaggaa gccgtgcgga tcctctgact cgcggaagcc cgccggatag gtgtggtcaa
      241 acaaaccaaa gagtctgtcg ccctgttgat ttacatgttg tctgtaagca gcactgaagc
      301 ccagccagcg acgcgggaaa atgcgcacac cgccgaggat atctacaggg ttgttaccga
      361 aagcattctc cgtgcgacca cggaagtatt gaatcgactt cacctcagtg atgatttgga
      421 aatgcttgtt gactgggaag tcgaagccga ctccagcaac caactcatca ggccgattta
      481 gaagtgagac gttcctcccg ccaaaagctt ccgaacgcgg attgctgtta agaatgaatc
      541 caacgttagc ggaaaggtta acgtgcttgg ccagacgtcc actgacgaaa ccgacagcgc
      601 caaagtcacc aatatcgcca ccggggctcg caccgcgctg caactggtta aagccagaaa
      661 agtcgtcagc cttgtccgga tagaagcgat agaaagctac taaaccaaca ccgagtggat
      721 tgttgggtcc agtgaaacga atcttgccac caaccgtgaa atctccaaac attgactcac
      781 catagagtcg attgatgaag ggagcatcgg cgatatacgt aggcgcgatc gtgtaaaggt
      841 ctggcacaac ctgcgcagcg aaagttaggt tcgcaggaat ggttcgggtc gtcagcacta
      901 caccgggaag gatgctgcct accggcgaac cgtaaccggg gaagaaggcc gcatcgccgt
      961 agtttccgcc gccgccagtc ggaacaccga gacggcttac gaacggtggc gctatggcgt
     1021 taccagtcaa accgaagttc ggtccaactc cgcccacgaa cggaaactgc acaaagcgct
     1081 gtccaccgac attacacggc gggccagcag gccggaaaac agtgccggta atattctgcg
     1141 tgccactcac gcgctccggt gccaaaatga tcgcttgcgg cgagcatctg agcgaagcgc
     1201 taaagagcag ctgcgagttt ggaaggtaaa agcccgacag gttccgcggg ttattcactt
     1261 tcactccgcg atagccgttc gtcttgaaaa acagctcgat gtaatcgttc aacccgatgt
     1321 tgaagctaag cggagtttcc acgatgtcga cgtcgccggg atcacgatcg tagttgctgt
     1381 aagcgatgct aaacgtgtac tcaccacgac gaatcgtgtc gccgtcgtag atcgtaaaca
     1441 ggccggttgg gccacccgga gggccgccgg ttcccacggt cggcgcctgg tttcgtgggt
     1501 cattttcttt ccttaaagtt tgtgctgata ccgcggcaca cagcaccaac atgacgagcg
     1561 cggtaaatag cgctctagtt gcgagtctca tcgcattcct cctgccaaaa tatgaaattt
     1621 gcttaatagc gttgaccagc gcaagcaacc acttgcagaa gcgggggctt cactttcaag
     1681 aaccaaagag ggcctcatgg cccatcgcgc acctcaaaag caccgcacga aatggtgaaa
     1741 tcttatcgct gggccgggtg ctaaaatcat gccgaccata ctctacctgt tagcggaaat
     1801 caagactttt tcactagtta gcgtcaactt cttttatgtt ttttccgtca catgtggtca
     1861 cgtgtcgtca cgcgtagatg cccctcatgc gggtgtcata cgccactcga tcgatggcca
     1921 acatgtaggc cgcggtccgc gtgtcgacat ggtgtgcagt cgcataacga cacagatcat
     1981 tgaaacttgc gaccatagta tcctgcagcc gctggttgac gacatcttcg cgccagaagt
     2041 agcccatcct gtcctgaacc cattcaaaat acgaaactgt gacaccgccg gcgttcgcca
     2101 ggatgtcggg tacaacgaaa acaccatttt gatgcaacac ttcgtctgcc gcggcggtgg
     2161 ttggcccatt ggccccttca gcgagcactt tgcacttgat tcgatgagca ttctgcgagg
     2221 tgatctgatt ctcagtggct gcggggacaa gcacatcaca ttcaagctct aatagttcgc
     2281 tgttcgaaac cgtctccaca ccttcatacc cttcgaatga cctggttgtc tgcaaatagt
     2341 cgagcgccgc cgggatgtct atgccctttg ggttgtagat tccgccgtgg acgtctgaaa
     2401 tggctacgac ctggtaacct gcttcgtgaa gtaagcgggc accaatcccg ccaacattgc
     2461 ctgacccctg aacaactact cgagtagttg tgggggtcat ctgaaaccgc ttaatggcct
     2521 cattgatcac aaacaaaatg ccgcgtcctg tggcttcacg ccgaccagaa gagccaccga
     2581 gatcaattgg cttgccggtc acaacggcgg tgactgtgtg acgcgcatgc attgaatagg
     2641 tgtccatgat ccaagccatg gtttgttcgt tcgtattcat gtcaggcgcc ggcacatctt
     2701 tttccggacc aatgaagtcg agcagctccg aagcatagcg tcgcgtcatt ctctccaact
     2761 cacccatgga catctgttgc ggatcgcaaa cgacgccgcc cttcgcaccg ccaaaaggaa
     2821 cattgacgac ggcgcacttc caagtcatcc aggcagcgag ggcgcgcacc tcgtcgatgt
     2881 tcacatcggg cgcatagcga attcctccct tggccggccc cctagcaaag ttgtgctgca
     2941 cgcgatagcc ttcgaagacc tgaatccggc cggagtccat gcgagtgggg atgtagactt
     3001 tcagctcccg attgggtacg cgcatcacgg catacaaatc cggatcgagg ttgaggatct
     3061 ttgccgcacg gtcgaaacgc gacatcatcg actcaaaggg atttgcctct tttatctccc
     3121 gaatgtcgtc aacgtacact gcattaggca tgctgcacat ttcttctcca attttatttt
     3181 agatcccttg ggcaagaatc tcgttcatcg cgccactgcg ataaccgtgt aagtctaaag
     3241 ttacatactt aaacccaagc tcacgaaagc ggcgcgctaa ttcgtcaacc atttcccgcc
     3301 gcaacgcgcg atccatctct gccggagcga tctcaagccg cacaatttga tcatgatgtc
     3361 gcacacgaaa ctcgcggaaa cccaacgaac gcaagatgtc ctctccctgg tcgacggtgg
     3421 tgagccgttc gatagtgacg tgagtgccgt aagcgatccg cgatgagaga caggggctgg
     3481 caggcttgtc ccaggtcgga aggccggcgc ggcgacttag ctcccgaacc tcgagcttgc
     3541 ttagacccac ctcgacgagc ggactgcgaa ctccatgctg gccagcggct actcttccgg
     3601 gacgatagtc aagcaggtcg tcggtggttg agccatcaag gacaaactga atctgacgtt
     3661 ctcgcgcaaa gggctcaagc ttctgataca actcgtcctt acagaagaaa caacgattcg
     3721 ggccgttggt aacgtattct gcgttttcta attcctgcgt ttgaattgtc tcaacgcgga
     3781 aaccaaattg cccggcaacg cgcgcggttt cgtctctttg gtaacttggc aggctggcgg
     3841 attccccggt cacacaaagt gcacgctcac caagctcaga gttagcaaca taggctacgt
     3901 aagtgctatc gacgccgccc gagaaagcaa c
//
LOCUS       DASUEX010000598         2878 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1694595, whole genome shotgun sequence.
ACCESSION   DASUEX010000598 DASUEX010000000
VERSION     DASUEX010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1694595"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="VFV61_09945"
     CDS             complement(<1..402)
                     /locus_tag="VFV61_09945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HEX4900844.1"
                     /translation="MAEHEEITIKATVREGRGKNDARRTRREGLVPITIYGGGGETVA
                     AAAPLSALAAVLRSEAGRNTIFTLDVEGVGLSEVMFHERQIDPVRGRLVHADFQRLVK
                     GQKIEVTVPLRLVGEPVGVREELGVLEQIMRE"
     gene            complement(592..1539)
                     /locus_tag="VFV61_09950"
     CDS             complement(592..1539)
                     /locus_tag="VFV61_09950"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016445698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEX4900845.1"
                     /translation="MSTTGGIKVFCGNANPKLAEEIAKHLGCDMGRAFAERFSDGEFN
                     FQIDENVRGGDVFIVQPTCPPTDAHLMELLVMLDAFRRSSAERITAVIPYYGYARSDK
                     KDRPRVPISARLVANLITVAGADRLLTMDLHAAQIQGFFDIPVDHLYSAPVMIGYYQD
                     NPLPNLTVVAPDTGGAERARAYAKRLDAELALCDKRREKANVAEVMNVVGDVRGRSCL
                     IVDDMCDTGGSLTKVAKALKEAGAGRIHACFTHPVLSGSAIEKLAESEIEKIIVTNTI
                     PLRDHACELKKIKVLSIAPLLAKAIKSIHEETSVSSLFV"
     gene            complement(1621..1695)
                     /locus_tag="VFV61_09955"
     tRNA            complement(1621..1695)
                     /locus_tag="VFV61_09955"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1661..1663),aa:Gln,seq:ttg)
     gene            complement(1713..2639)
                     /gene="ispE"
                     /locus_tag="VFV61_09960"
     CDS             complement(1713..2639)
                     /gene="ispE"
                     /locus_tag="VFV61_09960"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEX4900846.1"
                     /translation="MSNRPLAILSFAKVNLSLRILGKRPDNYHEIHTVLQAVSLHDTL
                     NFVTTSAGPIAFTCDDASIPVDDSNLVVKAADSLRERFRVMSSCKIHLTKRIPVQAGL
                     GGGSSNAAIILLALTRLWELPATSQDLLEMAANLGTDVPFFIEGGTALATGIGTELTP
                     LPDLPQQRLLIVAPAVTVSTRTAYAALKAPALTTSDSVSILAISRTQAIFSDSKQWAL
                     CDHLENDFERVIFDMEPEIRRVKEALIQAGARCALLAGSGSSVFGIFDSDEVLSSAAQ
                     ALKQESGWRIFSCVTLSRDEYLRALNSAIPGR"
     gene            complement(2693..>2878)
                     /locus_tag="VFV61_09965"
     CDS             complement(2693..>2878)
                     /locus_tag="VFV61_09965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900847.1"
                     /translation="IAENIAYNQGFEDPGAFAVERWTISEGHRANLLSTEFRASAVGS
                     FVASDGTVFLTQLFILR"
BASE COUNT          679 a          821 c          751 g          627 t
ORIGIN      
        1 ttcgcgcatg atctgttcga gcacgcctag ctcttctcga actcccacgg gttcgccgac
       61 gaggcgcagc gggacagtaa cttcaatctt ctgacccttc accaggcgct gaaagtcggc
      121 atgcaccaga cggccgcgca ctggatcaat ctgccgttcg tgaaacatca cttcactcag
      181 gcccacgcct tcaacgtcca aagtgaagat ggtgttacga ccagcttcgg agcgcagcac
      241 cgccgccagt gcgctcaagg gcgcggcagc agcaactgtt tctccaccgc cgccgtaaat
      301 ggtaatggga actaacccct cgcggcgcgt tctacgagcg tcgttcttgc cccgaccctc
      361 gcgcaccgta gccttgatcg ttatctcttc gtgctcagcc ataatcactc ctcattacag
      421 actttcagcg acgaccgatt ggcgatttgc gattggcgat tggcggttgg caagttttca
      481 agcttgagct aactgcaaag cttgaacagc tgctaatcgg cattcggcaa tcagcaatcg
      541 gaaatccgtc cgtcgctgat ggtcttcttt taagccatca gtgtttgccc ttcatacgaa
      601 gagcgaggaa acgctggttt cctcgtggat cgacttgatc gctttcgcca gaagcggcgc
      661 gatactaagt acctttatct ttttcaactc gcacgcgtga tcgcgtagcg gaatcgtatt
      721 cgtaacgatt atcttttcaa tctctgactc agccagcttt tcaatggcac tgccggagag
      781 taccggatgc gtgaagcagg cgtgaattct tccggcgccg gcttccttta atgctttcgc
      841 cactttggta agcgaaccgc cggtatcgca catgtcatcg acaatcaaac aactccggcc
      901 gcgcacgtcg cccaccacat tcatcacctc agcaacgttg gctttttcgc gtcgcttgtc
      961 acaaagcgcc agttctgcat ccagccgctt ggcgtaggcg cgcgctcttt ctgcgccgcc
     1021 cgtgtcagga gcaaccactg tcaggttcgg cagcggattg tcctgatagt acccaatcat
     1081 gaccggcgcg gaataaaggt ggtcgacggg aatatcaaag aacccttgaa tctgcgctgc
     1141 gtgcaaatcc atagtcaaca aacgatcagc gccggctacg gtgatcaggt tggcgacaag
     1201 tcgtgccgaa atcggcacgc gcggacggtc tttcttatca gaacgcgcat aaccgtaata
     1261 aggaatcact gccgtgatgc gttcggcgga ggagcggcga aatgcatcga gcatcaccag
     1321 cagctccatc aaatgcgcat cagtcggcgg gcaagtcggt tgcactatga aaacatcgcc
     1381 gccgcgcacg ttttcatcta tctgaaagtt aaactcgcca tcggaaaagc gttcggcgaa
     1441 agcccgcccc atatcgcagc caaggtgttt cgctatctct tccgctaact tggggtttgc
     1501 gttgccgcaa aaaaccttga tgccgccagt tgtgctcata gggaagtttc gagtctcaag
     1561 tttcgggttt cgagttagcg aggcccgcga aactcgtaac tcgtaactcg aaacaattat
     1621 tggctggggc gggaggattc gaacctacga atgccggttc caaagaccgg tgccttacca
     1681 cttggctacg ccccagtatc agaatccttc ggtcaacgcc caggaatagc agaattcaaa
     1741 gcgcgtaaat attcgtcgcg cgacagagta acacaagaaa agatccgcca accgctttcc
     1801 tgtttcaagg cttgagcggc gctgctgagc acctcatcgc tatcaaagat accaaatacg
     1861 ctcgaaccgc tcccagcgag cagggcgcaa cgagcgccag cctgaatcag tgcttccttc
     1921 acgcgccgaa tctcaggctc catatcaaag atcactcgct cgaaatcatt ctctaaatga
     1981 tcacacagtg cccattgttt cgaatcggaa aaaatagcct gtgtacgcga aatagcaagg
     2041 atagagaccg agtcggatgt tgtcaaggca ggtgctttca gcgctgcata cgcagtcctt
     2101 gtcgacaccg tcacggcggg agcgacgatt agtaagcgtt gctgtggtag atcaggaagc
     2161 ggcgtgagtt cggtgccaat acccgtcgcc aacgcagtcc caccttcgat gaaaaacggc
     2221 acatcagtac cgagattcgc agccatctcg agcaagtcct gggaagtcgc aggaagctcc
     2281 cacagcctcg tcagggccag caatatgatt gcggcgttgg atgatccgcc accaaggccc
     2341 gcctgtaccg gaattctctt cgtcaggtga atcttgcatg aactcatcac ccgaaatctc
     2401 tcccgcaggg aatctgccgc cttaactacg agattgctgt cgtctacggg aattgaggca
     2461 tcgtcgcacg tgaaagcaat cgggccagct gatgtcgtga caaagttaag cgtatcgtgg
     2521 agcgagacgg cctgtaggac cgtatgaatc tcgtggtaat tatcgggacg cttgcccagg
     2581 atgcgcaggc tgaggttgac cttggcgaaa gacagaatgg cgagagggcg gttgctcaca
     2641 agaaaaggcg gccgacgtcg cgccagccgc ctcttatcgt cttgtttggc gtttagcgca
     2701 aaatgaaaag ctgggtcaga aaaacggtgc cgtcagaagc aacaaaactt ccaaccgccg
     2761 acgcgcgaaa ttcagtgctg agaaggttcg cacgatggcc ctcagagatc gtccaacgct
     2821 cgacagcaaa ggcacctgga tcttcgaagc cttgattata cgcgatgttc tccgcgat
//
LOCUS       DASUEX010000599         2992 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1016758, whole genome shotgun sequence.
ACCESSION   DASUEX010000599 DASUEX010000000
VERSION     DASUEX010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1016758"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2128
                     /gene="alaS"
                     /locus_tag="VFV61_09970"
     CDS             <1..2128
                     /gene="alaS"
                     /locus_tag="VFV61_09970"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEX4900848.1"
                     /translation="ENCVANVNGPGDTIMEIWNLVFMQFQRSETEPGKFKLDPLPAPS
                     VDTGAGLERLAVVLQGVKSNYDTDLILPIIKFTATLADRHYESETKEGFAMRVIADHA
                     RATAFAIADGILPGNEGRNYVLRKIMRRAIYQGRHSLGFHDSFFNEVTNFVVDQMSAA
                     YPELESQRDFIKKMVGLEEQRFGTTLTVGLNKLEELFAARGQTMPDFKQLAKLYDTFG
                     IPRDLIRVALEERGFAVPEEEFNQDFDEAVRQIQLAGGTTSASAATKVVKEGYGRIAG
                     QGRSQFRGYDATQVEDARVVALLQEGSEVTALESGDEGEVILDQTPFYAESGGQVGDV
                     GRLLSTDFLASVSDTYSPVQGLIVHKIKVEKGTLKVGDQVSAEVDVEKRDATRRNHTA
                     THLMHAALREVLGSHVKQAGSVVAPTYLRFDFTHYQPLTPAEIEEIERLVNYHILRNE
                     PVQTNEMAIEEAMRSGAMALFGEKYGEKVRVLSINGVADVFSKELCGGTHVRATGDIG
                     LFKITSDESIAAGVRRIRAITGVDAFDRFREAEAIVDKVAADLRTTRTEVPVVISKLQ
                     DELKKARREADELRMKIATGAAGPAANGNEERDVAGVRVVAREASGLSANEMRELSDT
                     LLARLKSGVVVLGRSGDGKVSLIVRTSPDVSKRVPAGQVIKELAPIVGGKGGGKPDMA
                     EGGGNLPEKLSEALEASYRVIKNLLQ"
     gene            2398..>2992
                     /locus_tag="VFV61_09975"
     CDS             2398..>2992
                     /locus_tag="VFV61_09975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900849.1"
                     /translation="MIASRVLASLLLLFASTLTMAQESTSPRCGVILLDGNSTGTTDS
                     APAWRAAFEDLARCGGGTLDVGHGQYRLETTVTKDFLSLDSKITIRCQGSNSQVIFAT
                     GASADGLVLQNLEDLTIEGITFVGSPNLLPDGSLNPSAGSDVFRAINLQGIMRAKIQN
                     NMFYGIAAPAPGAAIIKAAASYLTVEHNQFLGSAACQS"
BASE COUNT          721 a          798 c          845 g          628 t
ORIGIN      
        1 ggaaaactgc gtcgcaaacg tcaatgggcc gggcgacact atcatggaga tttggaatct
       61 cgtcttcatg cagttccagc gtagtgaaac agagccgggg aaattcaaac tcgatccgtt
      121 gccggcgccc tccgtcgata ctggagctgg tttggaacgg ctcgcagtcg tcctgcaagg
      181 cgttaagtcg aactacgata ccgatctgat tcttccgatc attaagttca cggcaacgct
      241 tgccgatcgt cattacgagt cggaaacaaa ggaaggattt gcgatgcggg tgatcgccga
      301 tcacgcgcgc gcgacagctt tcgcgatcgc cgatggcatc ctgcccggta atgaaggacg
      361 gaattatgtg ttgcgcaaga taatgcgccg ggcgatttat caaggcagac actcactcgg
      421 gttccacgac agcttcttta acgaggtcac caactttgtc gtcgatcaaa tgagcgcggc
      481 ctatccggaa ctggagagcc aaagagactt catcaagaag atggtgggac tggaggagca
      541 acgcttcggc acaaccttga cggttggttt gaataagctc gaagaactgt ttgcggccag
      601 aggtcaaacg atgccggact ttaagcaact ggcaaagcta tacgacacct ttggtattcc
      661 ccgtgatcta attcgcgtgg cgctggagga acgcgggttc gcggtgccag aagaagaatt
      721 taatcaagac ttcgacgagg cagtgcgaca gattcagtta gctggcggta cgacttcagc
      781 atccgcagcc accaaagtag taaaagaggg ttacggcagg attgccggac agggccgaag
      841 tcagtttcgc ggatacgatg ctacacaggt cgaggacgcg cgtgttgttg ctttgctaca
      901 ggaaggcagc gaagtcaccg cgctggaatc cggcgacgaa ggcgaagtca tacttgatca
      961 aacacccttc tacgccgaaa gcgggggaca ggtaggggac gtcgggcgtc tgctgagtac
     1021 tgacttcctt gcttcggtca gcgacaccta ttcgcctgtg cagggcttaa tcgtgcacaa
     1081 gatcaaagtt gaaaagggaa cccttaaagt cggcgaccaa gtttcagcag aggttgatgt
     1141 ggaaaagcgc gatgccacgc ggcgcaatca cacggcaact cacttgatgc atgccgcgct
     1201 gcgtgaagtt ctcggcagtc acgtgaaaca agccggctca gtggtcgcac caacctatct
     1261 gcgtttcgac ttcacgcatt atcaaccgct gacgccagcg gagatcgaag agattgagcg
     1321 cctcgtgaac tatcacattc tgcgcaatga accggtgcaa acgaatgaga tggcgatcga
     1381 agaggcgatg cgttccggcg cgatggccct cttcggtgaa aagtatggcg agaaggtgcg
     1441 cgtgctttcg atcaatggtg tggccgacgt cttctctaag gaactctgcg gtggcactca
     1501 cgtgcgcgcc acgggagaca tcggactctt caagattacc agcgacgaat caatcgctgc
     1561 gggcgtcaga cgcattcgcg ccatcaccgg agtggacgcg tttgatcgtt ttcgcgaagc
     1621 ggaagcgatc gtcgataaag tcgccgccga tctgcgcacc acgcgaacgg aggttcctgt
     1681 agtaatcagc aagcttcagg acgaacttaa gaaagcgcgg cgcgaggccg acgagttacg
     1741 catgaagatc gcgacgggcg cggccggtcc tgctgcaaac ggtaatgagg aacgggacgt
     1801 agccggcgtt cgtgtggtag ctcgtgaagc cagcggactc tcggccaacg aaatgcgcga
     1861 gctttctgac accttgctcg cgcgcctcaa atcaggagtc gttgtcctgg gacgcagcgg
     1921 cgacggcaag gtatcactga ttgtgcgcac ctcgcccgat gtctcgaagc gagttcctgc
     1981 aggccaggta atcaaagagc tggcaccgat cgtcggcggc aagggcggcg gcaaacctga
     2041 catggccgag ggcggcggaa atctgcccga gaagctatcc gaagctctgg aagcaagtta
     2101 ccgggtaata aaaaacctct tgcaataagc gctcgaacgc gcttagagtg aaagtggcct
     2161 cgcgttcgaa ctcctcgaca cttcgaacac caacaaaagc aatttgactc ttatctgggc
     2221 aggcctttta aagtcctgct caagatccga tcaccgagtc gcttcacaaa ctgacacgaa
     2281 taccgtgtct tttgagtctc cccaattctc acctcggaag gttctaacca cagactttcc
     2341 cgaattttac ccgctgtgcc tcccacttta gctgtgcgca gcatccagga gctcacaatg
     2401 atagccagcc gagttttagc atctctgctt ctactatttg cctcaaccct cacgatggcc
     2461 caggaaagca catcgccaag atgtggtgtc atcttgctcg atggtaattc cactggcacc
     2521 actgattccg cgccagcctg gagggcagcg ttcgaggatc tcgccagatg tggcggcgga
     2581 acactcgatg tcggtcacgg acaatatcga ctcgagacca ctgtgactaa ggattttctg
     2641 agtcttgaca gcaagattac aattcgctgt cagggctcaa acagtcaggt gatttttgct
     2701 accggcgcct ctgctgacgg cctggttctg caaaacctcg aagaccttac gattgaagga
     2761 atcactttcg tcggttcgcc aaacttgtta ccggatggct cgctaaatcc gtcagctgga
     2821 agcgacgtat ttcgcgcgat aaatcttcag gggattatgc gagccaaaat tcagaacaac
     2881 atgttctatg gtattgccgc accggcacca ggggccgcaa tcattaaagc cgcggcctcc
     2941 tacttgacgg tcgagcataa tcagtttctg ggaagtgccg cctgccagtc ag
//
LOCUS       DASUEX010000600         2709 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_318566, whole genome shotgun sequence.
ACCESSION   DASUEX010000600 DASUEX010000000
VERSION     DASUEX010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2709
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318566"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..604
                     /locus_tag="VFV61_09980"
     CDS             <1..604
                     /locus_tag="VFV61_09980"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX4900850.1"
                     /translation="VFLTSRLLEPLYNVGCVLRAFQVIQRRYPKARLTVAADGWMRGE
                     LEDLARSLELRDTKFIGRVLFEEMPAMYDSADIYLTATNLDNMPSSITESMASGLPVV
                     TTDAGGIPYIVTHEQTCLMVSCNDHEAMAAAALRLLEDPDLAIRIGKQARDAARKFTW
                     PAVRDEWVKLYHELFAEQSAGEMPAEDRHQNKAQIELGPM"
     gene            651..>2709
                     /locus_tag="VFV61_09985"
     CDS             651..>2709
                     /locus_tag="VFV61_09985"
                     /inference="COORDINATES: protein motif:HMM:NF028199.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HEX4900851.1"
                     /translation="MSTVGHKFKKLRRLDFDEVRVRGAQKLSALAERHGWSSLGHLPT
                     DNAFLKLLDGTRQQADEYLEHFRSRTRPAFFAGFDNREATSRVLNSRWPLFAADLVKR
                     ADRITEGRFDLLGLEQLSFGNPIDWHLEPIAGKRAPLVHWSRVDELDAERSGDKKILW
                     ELNRHQHFVILGQAYWLTNDERYAKAFVSQLSSWMDQNPPKLGIHWTSSLEIAFRSIS
                     WLWSFYFFRESPSFSPQLLWRVLKFLYLNARHLETYLSTYFSPNTHLTGEALGLFYLG
                     TLLPEFREAERWKDTGRRILLDQLPIHVRPDGVYFEQSSYYHRYTTDFYAHFLLLSRT
                     NGETLPGVEAKLQSLLDHLMYITRPDGTTPFFGDDDGGRLMVLDDRPANDFRAALSTG
                     AAVFGRADYKFVAREAAQETLWLLGPNGLPQFDEILAAEPPQRSIAFADSGYYVMRDG
                     WDREANYLLFDAGTHGVLNCGHAHADALSLELVARGRTELVDPGTYSYTGSREMRDWF
                     RSSAAHNTLTIDGESSSVPDNTFSCKTTADAQCSQWISRDRFDFVTGTHEGYKRLADP
                     VEHSRSILFLHQDYWVIRDQLRSEREHRADLWFHFEAGTAPLIEAEEGAPVTLGEHSG
                     ETGLDIVSFGQNGNWRREEGWVSHWYGSREPARLYVFSGKTTKDDQFVTFLLPAGHAS
                     ERKA"
BASE COUNT          604 a          730 c          739 g          636 t
ORIGIN      
        1 cgtttttcta accagccgct tacttgagcc actctacaac gtgggctgtg ttctgcgggc
       61 ttttcaagtg atacagagac gctatccgaa agcgcgtttg accgtggcgg ccgatggatg
      121 gatgcgcgga gaacttgaag atctcgcacg ctcgcttgaa ttgcgcgaca cgaagtttat
      181 cggtcgcgtt cttttcgaag aaatgcctgc gatgtacgac agcgcggaca tctatctgac
      241 ggctacaaat ctcgacaaca tgcccagctc aattacagaa agcatggcgt caggcctgcc
      301 agtagtaacc acagatgccg gcggtattcc gtacattgtg acgcatgaac agacctgttt
      361 gatggtctct tgcaacgatc atgaagccat ggcggcggcg gccctgcgac tgctggaaga
      421 tcctgacctg gcaattcgca ttggcaagca ggcaagagat gcggcgcgca agtttacctg
      481 gcccgcggtt cgtgacgagt gggtcaagct gtatcacgag ttgtttgccg agcagtcagc
      541 gggcgagatg ccggcggaag atcgacacca gaacaaggcg cagattgagc tcggaccaat
      601 gtgatttttc ttcgcagttc tcaaacagtt tcctgccctg agtaatattg atgtccactg
      661 tcggccacaa gtttaagaaa ctccgcagac tagacttcga cgaggtccgc gtccgcggag
      721 cacagaagct ctcggcgctg gcagaaagac acggatggtc ttcgttgggc cacctgccca
      781 cagacaacgc tttcctgaaa ttgctcgacg gcactcggca acaggctgac gaatatcttg
      841 agcacttccg ttcacgcact cgccctgcct tctttgcagg atttgataac cgggaagcaa
      901 cctccagagt attaaactcc aggtggcctc tatttgcggc cgacctggtg aagcgcgccg
      961 accgcattac ggagggaagg ttcgacctac ttggtctcga gcaattgagc tttggcaacc
     1021 caatcgactg gcacctggaa ccgatcgcgg gaaaacgcgc tccgttggtt cactggagcc
     1081 gtgttgacga gctggatgcg gaaaggtcgg gcgacaagaa gattctatgg gagctaaacc
     1141 gtcaccagca cttcgttatc ctcggtcaag cttactggct cactaacgac gagcgttatg
     1201 ccaaggcctt tgtttcacaa cttagttcat ggatggacca gaatcctcct aagcttggga
     1261 tccattggac cagcagcctg gaaattgcct ttagatcgat ctcgtggctg tggtcgttct
     1321 atttcttcag agagtcgccg tcattctcgc ctcagttgtt atggcgagtt ctaaagtttc
     1381 tctatctcaa cgcccggcat ctggaaacct atctttcaac ttacttcagt cccaacacac
     1441 atctgacggg ggaagcgctc ggcctgtttt atctgggcac cctccttccc gaattcagag
     1501 aggccgagcg ttggaaagac actggcaggc ggatcctgct tgatcaactt ccaattcatg
     1561 tgagaccaga cggagtctat ttcgaacagt cgagctacta tcatcgatac acgaccgact
     1621 tttatgccca ctttcttctg ctctcacgga cgaacggcga gacattgccc ggggttgaag
     1681 cgaaacttca gtccctgctt gatcacttga tgtacatcac ccgtcccgat ggcaccacgc
     1741 ctttctttgg tgatgacgac ggtggacgat tgatggtgct ggacgatcgt ccggcaaatg
     1801 attttcgcgc tgcactttcg acgggcgcag cagttttcgg aagggccgat tacaagtttg
     1861 ttgctcgcga agcagcacag gaaaccctgt ggttgctagg tccgaacggc ctgccacagt
     1921 ttgacgaaat tcttgccgca gagccgccgc aaagatccat cgcctttgca gacagcgggt
     1981 attacgtcat gcgcgacggt tgggatcgag aggcaaacta ccttttgttc gacgctggga
     2041 cccatggtgt tctgaactgt ggtcacgcac atgcggacgc attgtcactt gaactggtgg
     2101 cgaggggccg cactgaactg gtcgatccgg gaacgtacag ttataccggt tcaagggaga
     2161 tgcgggattg gtttcgcagc tctgctgctc acaatacgct aactatcgat ggtgaatcgt
     2221 cttcagttcc cgacaacacg ttttcctgta agaccactgc cgatgctcag tgttcacagt
     2281 ggattagccg tgaccgcttc gactttgtca cgggtacgca cgaaggctac aaacgattag
     2341 ccgatccagt cgagcactcc cgcagcattc tctttctcca ccaggactac tgggtcattc
     2401 gcgatcaact gcgctcggaa cgtgagcacc gggcggacct ttggtttcat ttcgaagccg
     2461 gaactgcgcc gctaatcgaa gcggaagagg gagcaccagt aactctcggt gaacacagtg
     2521 gtgaaactgg actcgatatt gtttcctttg gccagaacgg caattggcgc agggaggaag
     2581 gttgggtctc gcattggtat gggagtaggg aaccagcgcg tctttacgtc ttctcgggaa
     2641 aaaccaccaa agacgatcag ttcgtcacct tcctgttgcc cgctggccat gcttctgagc
     2701 ggaaagcgc
//
LOCUS       DASUEX010000601         2259 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1695709, whole genome shotgun sequence.
ACCESSION   DASUEX010000601 DASUEX010000000
VERSION     DASUEX010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1695709"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="VFV61_09990"
     CDS             <1..219
                     /locus_tag="VFV61_09990"
                     /inference="COORDINATES: protein motif:HMM:NF019339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX4900852.1"
                     /translation="ELLGDKKVQESISFFRLNVFAFPRSANAYDSLGESYLEAGNQAL
                     AIENYRKSLELNPDNRNAEQVLKRLANK"
     gene            423..>2259
                     /locus_tag="VFV61_09995"
     CDS             423..>2259
                     /locus_tag="VFV61_09995"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX4900853.1"
                     /translation="MTPERWRQVEDIFQAALDLNTEERARYMTVACRGDAALRRDVES
                     LLQQHESGGGQTYSADPDVIESLEELEDPLLGVRLGAYLIEREVGRGGMGAVYEARRV
                     DQEFNMRVAIKLVKRGMDTDFILRRFRRERQILAALTHPNIALLLDGGTTADGLPYFV
                     MEYIEGQPLYLYCDEHQLSISERLKLFQSICDAIHYAHQKHIVHRDIKPSNVLVTADG
                     IPKLLDFGIAKLLNPDIAGDMTHDPTATAMRLMTPEYASPEQVQGAPTSPTTDVYSLG
                     VLLYELLTGHRPYRLRNRAPHEIARVICEEIPPPLSVVITRQEDLLPSGPVTEDATTL
                     TALYTARRSTPEGLHREFSGDLDEIVMRALRKEPEWRYQSVEQLRNDISAYLEGRAVS
                     SLPDAPFSASGPKFDPSNQENSLAVLPLKLFDLDQGTGSGPDYLGTGLADALIARLGG
                     VRRFAVRPTSSVLRFGTDSDPLVAGRELGVAFVLDGRIRRAGDQIRVTVQLLNVQNGT
                     AVWAGQFDEKFTDVLSLEDAISANVAESLVPQLSGDERRRLAKRGTNDPQAHEAYLRG
                     RFYWNTFTEDGFARAIVCYHQAIALDPHYAVAYAGIASYYNWLGSF"
BASE COUNT          485 a          619 c          635 g          520 t
ORIGIN      
        1 gaattacttg gcgataagaa agtgcaggaa tcgatctctt tcttcaggct aaatgttttc
       61 gcctttccgc gctccgcgaa cgcgtacgac agtttgggcg agtcttacct ggaagctgga
      121 aatcaggcct tggcgataga aaactatcga aaaagtttag aactcaatcc ggacaatcgt
      181 aacgctgagc aggtactgaa acggctggca aacaagtagc ctcactcgat cccggattcc
      241 ctcctaacgc ctattttgga gcgagcaaaa aatttggtta caattgacgc gcgagttttt
      301 gaataagtgg gttagcgcca gccagtattg agaaccatgc gacacccgaa cgtatagttc
      361 cgaatactca ggcgcttcgt tttctcccgg gtttatcaag caatcaatca atacaactgt
      421 taatgacccc cgaacgctgg cgacaagttg aagatatttt tcaggcagcg ctcgatctca
      481 atactgagga acgagcccgg tatatgaccg tcgcatgccg gggcgacgcg gcgctcaggc
      541 gtgatgttga atcgctattg cagcagcatg aaagtggcgg cggtcaaacc tactcagcag
      601 atccggatgt gattgagtcg ctcgaggagt tggaggatcc cctcctcggt gtacgcctcg
      661 gcgcctacct cattgaacgg gaagtcgggc gcggcggcat gggtgcggta tacgaggccc
      721 gccgcgtcga tcaggaattc aacatgcgcg tggccattaa attggtcaag cgtggcatgg
      781 ataccgactt tatcctccgt cgcttccgca gggaacgaca gatcctcgcg gcgctaaccc
      841 atccgaacat tgcgctcctg ctcgatggcg gcactactgc ggacggtttg ccgtacttcg
      901 tgatggagta catcgaggga cagccgctgt atctctactg cgatgaacac cagctcagca
      961 tttcggagcg tttgaagttg tttcagtcga tctgcgacgc catccattac gctcatcaga
     1021 aacatatcgt ccaccgcgac attaaaccgt cgaatgtact ggttacggct gacggtattc
     1081 ccaagctgct cgattttggc atcgctaagt tgctcaaccc cgatattgcc ggcgacatga
     1141 cgcacgatcc cacggcgaca gccatgcgcc tgatgacacc tgagtatgct tcaccagagc
     1201 aagtgcaagg tgctcccacg tcacccacta cagatgttta ctcgctgggc gtgttgctct
     1261 acgaactctt aaccggccac cggccctatc gcctgcgtaa tcgggcgccg catgagatcg
     1321 cgcgcgtgat ctgtgaagaa attccgccgc cgctgagtgt ggtgataaca cgtcaggagg
     1381 acttgctgcc gagcggcccg gtgactgagg acgcgacgac cttgactgcg ctttatacgg
     1441 cgcgccgctc tactcccgaa gggctgcatc gcgagttctc aggagatctg gatgagattg
     1501 tgatgcgagc gctacgcaaa gagcccgaat ggcgttacca gtctgtcgaa cagttgcgca
     1561 acgacatcag tgcttatctg gaagggaggg cggtttcttc ccttcccgat gcgccgtttt
     1621 ctgcatcggg gccgaaattc gatcccagca accaggaaaa ttcactggcg gtattgccac
     1681 tgaagttatt cgatttggat cagggaaccg gctctggtcc tgattatctt ggtaccggtc
     1741 tggcggatgc tttgattgcg cgcctgggtg gcgtccgacg ttttgccgta cgcccgacca
     1801 gcagcgttct gcgtttcgga actgactctg atcccctggt ggccgggcgg gaactgggcg
     1861 ttgctttcgt gctcgacggt cgcatccggc gtgcggggga tcaaattcgc gttacggtac
     1921 aactgctgaa tgttcagaac ggcactgcgg tttgggcggg acagtttgac gagaagttta
     1981 ccgacgttct cagtcttgaa gacgcgattt ccgcgaacgt tgcggaatca ctggtgccac
     2041 aactgagcgg cgatgagcgc cggcgactgg ccaagcgcgg cacgaatgat ccgcaggctc
     2101 atgaagctta cctacgtggt cgcttctact ggaacacgtt caccgaagac ggatttgcgc
     2161 gcgctatcgt ttgttaccac caggccatcg ctcttgatcc ccactatgcg gtcgcctatg
     2221 ccggcatcgc ttcttactac aactggctgg ggagtttta
//
LOCUS       DASUEX010000602         4376 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1695788, whole genome shotgun sequence.
ACCESSION   DASUEX010000602 DASUEX010000000
VERSION     DASUEX010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4376
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1695788"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..2266
                     /locus_tag="VFV61_10000"
     CDS             137..2266
                     /locus_tag="VFV61_10000"
                     /inference="COORDINATES: protein motif:HMM:NF019552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HEX4900854.1"
                     /translation="MSIFRKLRRALRGQVSPRTALLESARRVYVASSLRGERLKLQQQ
                     AKQGARLTSKFAELPADTLLNHFRQRTEPHFFPGFDLPLQTIAAYQRVVFPDDSGTVL
                     VNAERILNAHAWPLLGLSDFTFVSWHRDPLSGYEWPLDLYRDIKLHRNDSSDVRLVWE
                     VNRLGHFITLARAYALSGDERFSNEFFRQLQNWETQNPIGFGVNWNCAMEVAFRALNL
                     LGAFAAFRRAHGLSSEQLARLLRVLDQHGRFICEHLEFSYVVTSNHYLTNVVGLLWLG
                     IMLPELDDAAGWRDFGLRELLREMEKQVLPDGADCESSTGYHRLVLELFSYSFLLCRT
                     NGIEIEARYWEKLHRMFGYLRAYLRPDGYAPLLGDTDGSQVLPICRRSGNDQAYLLAI
                     GAILFDDPQLKKQECPPPPELFGLFGEQGVSKYQTLSSENAGTVSQQFPDAGVYVLRD
                     GDQYLLFNASDAGLNGRGSHGHNDALSIEVSACGIAFIVDPGTYVYTADLRQRHLFRS
                     TAYHSTVQIDETEQNSTDEKLPFVIGNEAAPRLLEWNIGPEREVVAAEHYGYRRLPQP
                     VTHRRKVVFNKKRRAWSMLDKFEGEGVHDLSFRFHFAPGLKIATKGPNSLGNEFPQTE
                     FLELLDESSGARLLIAGYDTDSPVARPPKLEDRFSSKDYGAKEASQSAHWSLRTRLPF
                     AFSFVIVPVCSGENEIERFALAISWDD"
     gene            2268..4196
                     /gene="asnB"
                     /locus_tag="VFV61_10005"
     CDS             2268..4196
                     /gene="asnB"
                     /locus_tag="VFV61_10005"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEX4900855.1"
                     /translation="MCGICGVWEPGATERRVETALLGRMRDQMQHRGPDDAGELVFDE
                     GRGGFGFRRLSIIDLSAAGHQPMHGCTNKLWLVFNGEIYNHLPLREGLEARGHRYTSR
                     TDSETILHLYEERGLDFVDELEGDYGIALWDAEREQLVLVRDRIGVKPLYYYYENGRF
                     IFASEIKAILEHPAVTAEVDEPSLYHYLTFLTTPAPQTLFKNIRKLPAGHTLVAKRNG
                     TIEIRQYWDALPPLTTKQRTDDQHKVEILRLLRASITKRMMSDVPFGVFLSGGVDSSA
                     NVALMSEQMSRPVETFTVGFSDAEYLNELESARRISRQFQTNHHEVIISEKEMQSFLP
                     ELVFHQDEPIADPVCVPLYYVSKLARDSGTIVVQVGEGADEIFSGYDNYVRHLRIYEN
                     FWQHTEAVPAGIRRAISGVTRPALEATGRKRQAIELLRRWGADEPLFWGGAVVYDETF
                     KRRVLSTELRERFAELSSLEVVNRYLDHIERERPQSDFLARMTYLELKLRLPELLLMR
                     VDKITMATSVEARVPFLDHHLVEYAMGLPRALKVSGTSGKHILKRALEEVLPRDLLYN
                     RKRGFGAPVREWFRDGLGSWFDSHLMNSPMRKREFLDYRFVEHLLAEHRRGQRDWGFH
                     LWALLNLSLWYERWIDRG"
BASE COUNT          940 a         1129 c         1267 g         1040 t
ORIGIN      
        1 aaattaccgc agcgtcgttt gcggctttgg atcgggatcc cgcgcgcagc catcgcatcc
       61 ctccattgtg ggacggtcac accgctgatc gcatccttga cgccttgctg taagctgacc
      121 gccggaatcc tgccggatgt ctatttttcg caaactacgg cgtgccttgc gaggacaagt
      181 ctcgccgcgc acagcactgc tcgaaagcgc gagacgtgtg tacgttgctt caagtctgcg
      241 gggcgaacgg ctcaagctgc aacagcaggc gaaacaggga gccagactga caagtaagtt
      301 tgcggagcta cccgcagaca cattacttaa tcactttcgc caacgcactg aacctcattt
      361 cttccccggt ttcgacttac cacttcaaac gatcgccgct tatcagcgcg tcgtttttcc
      421 ggatgattct gggacggtgt tagtgaatgc ggagcgcatc ctgaatgcgc atgcctggcc
      481 gctgcttggc cttagcgact tcaccttcgt tagctggcat cgtgatccgc tttcagggta
      541 tgaatggccg ctagacctct accgcgacat caaacttcac cgaaacgaca gctcggatgt
      601 gcgtttggtg tgggaggtca accgtctcgg ccacttcatc acgctcgcgc gagcttacgc
      661 actcagtggt gatgagcgtt tcagcaatga gttctttcgg cagcttcaaa actgggagac
      721 gcaaaatcca atcggcttcg gagtcaattg gaattgtgcg atggaagtgg cgtttcgtgc
      781 gctgaatctc ctgggcgcct tcgccgcctt tcggcgggca cacggtttgt ctagcgagca
      841 gctggcgcgg ttgctcagag tccttgatca gcacggacgc ttcatttgcg agcatctgga
      901 attctcctac gtcgtcacca gcaatcatta ccttaccaat gttgtcggtc tattgtggtt
      961 ggggatcatg ctccccgaac ttgatgatgc cgcgggttgg cgcgactttg gattacgtga
     1021 actcctccgg gaaatggaga aacaggtctt gccggacgga gcggactgcg agagctcgac
     1081 tggatatcat cgtctagtac tggagctgtt cagttattca tttttgctgt gccgtacaaa
     1141 tgggatcgag atcgaggcgc gttattggga gaagctccac cgaatgtttg gttatctaag
     1201 ggcttatttg cggcccgacg gttatgctcc gctattggga gatacagacg gcagccaggt
     1261 tctcccaatc tgtcgacgta gcggaaacga tcaagcgtat ctactcgcca tcggagctat
     1321 tcttttcgat gatccgcaac tcaagaaaca ggaatgcccc cctcctcccg aactgtttgg
     1381 tcttttcggt gaacaaggag tcagcaaata tcagacgttg agcagtgaga atgccggaac
     1441 ggtctcgcag caatttcccg atgccggagt ttacgtgctc cgggacggtg atcaatatct
     1501 attgttcaat gctagcgatg cgggcctcaa cggtcgtgga tcgcatggac acaacgatgc
     1561 gttaagcatc gaagtctccg cgtgcgggat cgcctttatc gtcgatccgg gcacgtacgt
     1621 ctatacggcc gatctgcggc agcgacactt gttccgttcg accgcctatc attccacggt
     1681 gcagatcgat gagacggagc aaaactcaac tgatgaaaag ctgcccttcg taattggcaa
     1741 tgaggcagcg ccgcgtttac tcgaatggaa tattggtccg gaacgtgagg tcgttgcggc
     1801 tgagcattac ggttatcgac ggctgcccca gccggttacg catcggcgta aagtagtatt
     1861 caacaaaaag cgtcgggcgt ggtcgatgtt agacaagttc gagggcgaag gggttcacga
     1921 tctctctttt cgttttcatt tcgcaccggg attgaagata gccaccaagg gtcccaactc
     1981 tttaggcaac gaattccccc agacggagtt tctcgaactg ttggacgagt ctagtggcgc
     2041 ccgcttgcta atcgcaggct atgacacaga tagtccggtg gcgcgtccgc ctaaattgga
     2101 ggaccgcttt tcatcgaaag attatggagc gaaggaagcc tctcaatcgg cgcactggtc
     2161 tttgcgaacc cggttgccct tcgcatttag tttcgtcatt gttccagttt gctcaggaga
     2221 gaatgagatc gagcggtttg ctttggccat aagttgggac gactgagatg tgtgggatct
     2281 gcggagtctg ggaacctggc gcaactgaga gaagggtcga gacggccctg ctcggtcgta
     2341 tgcgcgatca gatgcagcat cgcgggcctg acgatgctgg cgaactagtc tttgacgaag
     2401 gccgcggtgg ttttggtttc cggcggcttt caatcatcga tctctcagcg gccggacacc
     2461 agccgatgca cggctgcaca aacaaactgt ggctggtatt caacggcgaa atctataacc
     2521 acctcccttt gcgagaaggg ctggaagcgc gtggtcatcg ttacacttcg cggactgact
     2581 cagaaaccat cctgcatctt tacgaagagc gcgggctcga ctttgttgat gagctcgaag
     2641 gtgactacgg aattgcctta tgggatgcgg agcgtgagca acttgtgctc gttcgcgatc
     2701 gcatcggcgt caagccgctc tactattatt atgagaatgg tcgcttcatt ttcgcttcgg
     2761 aaatcaaagc gattctcgaa catcctgcag ttaccgccga agttgacgag ccttcgcttt
     2821 atcattacct gacctttctg accacccctg cgccgcagac actcttcaag aacatcagga
     2881 agttgcctgc cggtcacacc ctcgtagcga agcgcaatgg cactatcgag attcgacagt
     2941 actgggacgc cttaccacca ctcacgacga agcaacgtac cgacgatcaa cacaaagtcg
     3001 aaattctgcg gctcttacga gcgtcgatta caaaacggat gatgtctgat gtgccgttcg
     3061 gagttttctt gtcgggtgga gttgattctt cagccaacgt cgctttgatg tccgaacaga
     3121 tgtcacgacc agttgagact tttacagttg gctttagcga tgctgagtat ctcaacgaac
     3181 tcgagtcagc acgacggatc tcacggcagt tccagacaaa ccatcatgag gtgatcattt
     3241 cggaaaagga gatgcagagt tttcttcctg agctcgtgtt tcaccaggac gagcccattg
     3301 ccgatccggt ttgcgtgccg ctttattacg tttctaaact cgcgcgcgat tccggaacaa
     3361 tcgtggtgca ggttggcgaa ggtgccgacg agatcttcag cggctatgac aactacgtgc
     3421 ggcacctgcg catttacgaa aacttctggc agcacaccga agccgtgccg gcgggaattc
     3481 ggagggcaat ttccggcgtt acgcgtcctg cgctcgaagc gaccggcagg aaacggcagg
     3541 cgatagaact cttgcggcgc tggggagctg acgagccact cttctggggc ggagccgttg
     3601 tctacgacga gactttcaaa cgtcgcgtgt tatcgacgga gttacgtgaa cgctttgccg
     3661 aattatcgtc gttggaagtg gtgaaccgct atctggatca cattgaacgt gaaagaccgc
     3721 agtccgattt tctggcacgc atgacctatc tggagttgaa acttcgctta cctgagctgt
     3781 tgctgatgcg agtagataag atcacgatgg cgaccagtgt cgaagcgcgc gtgccatttc
     3841 tggatcatca cctggtggaa tatgcgatgg ggttaccgcg ggcgcttaaa gtgtcgggca
     3901 cgagtggcaa acacatcttg aaacgcgcgc tagaagaggt tttaccgcgt gaccttctct
     3961 acaatcgcaa acgcggtttt ggtgctccgg tgcgcgaatg gtttcgcgat ggcttggggt
     4021 cgtggtttga ttcgcacctg atgaattcgc cgatgcgcaa gcgtgagttt ctggactaca
     4081 gatttgtgga gcacttgcta gcggaacacc ggcgcgggca gagggattgg ggctttcacc
     4141 tttgggcttt gcttaacctg agtctttggt atgaacgatg gattgatcgg ggttgaaggg
     4201 gggagaaggg gacgcgggga cgcggagaca cggggacgcg gggacgcgga gacacgggga
     4261 cgcggggata gggagaaggg gagacgtggg cagtccccaa cgtaccatga aagatctcag
     4321 actcggcttc gccaccaggt gtgaggcggt ggctctgccc cgccgtagga gctttc
//
LOCUS       DASUEX010000603         3204 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_443010, whole genome shotgun sequence.
ACCESSION   DASUEX010000603 DASUEX010000000
VERSION     DASUEX010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3204
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443010"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /gene="pip"
                     /locus_tag="VFV61_10010"
     CDS             complement(<1..611)
                     /gene="pip"
                     /locus_tag="VFV61_10010"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HEX4900856.1"
                     /translation="MRTLYPALEPFDTGRLQVSSVHNLYYEQCGNPKGEPVVFLHGGP
                     GGGIVPEYRQFFDPANYRIVLFDQRGAGQSTPHANLEANTTWDLVADIERLRLHLGVD
                     RWLVFGGSWGSTLSLSYAETHPDRVTALILRGIFLCRPHEIDWFYQEGHGASAVFPDI
                     WEEYVRIIPEAERGDLLRAYHKRLMSDDEAVRMEAAKAWSIWEG"
     gene            complement(1517..1885)
                     /locus_tag="VFV61_10015"
     CDS             complement(1517..1885)
                     /locus_tag="VFV61_10015"
                     /inference="COORDINATES: protein motif:HMM:NF044994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HEX4900857.1"
                     /translation="MDKRKVKGFRDKLLARREGLVGQVQQAEMYSRERDAEATQDPAD
                     MAANAYTKELLVSMSDNDRQLLNLIDEALERIEDGEYGHCVRCEEALTEKRLEAVPWA
                     RHCVRCQDLQERGLLTDEED"
     gene            complement(1950..2411)
                     /locus_tag="VFV61_10020"
     CDS             complement(1950..2411)
                     /locus_tag="VFV61_10020"
                     /inference="COORDINATES: protein motif:HMM:NF014629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEX4900858.1"
                     /translation="MRVHLVWPGKTRNAHLRALQDDYFQRLSHFARCEATELRANTRA
                     ATSPGIDRESRSISDALLDDEVTVLLDPAGREWSSEELARQIQKWETGGTKAVAFIVG
                     GPDGVSEELKVRVDKRWSLSRLTLTHEMARVLLLEQLYRAYAIIHRLPYAK"
     gene            complement(2411..>3204)
                     /locus_tag="VFV61_10025"
     CDS             complement(2411..>3204)
                     /locus_tag="VFV61_10025"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX4900859.1"
                     /translation="TLPSDLLEAELFGYERGAFTSAMEAKPGRLEAAHKGTLVLDEIA
                     YLSTDSQAKLLRVIETREFERLGGRRTIGVDARLIALTNVNLVQAVQRKNFREDLFYR
                     LNVVHIHVPPLRERPEDLPKLIEHFVLSYAGKHGREVMALAPEAMSLLQEYEFPGNIR
                     ELSNTIERAVIVCTGSRIQVGHLPEAIRAAVQLQRRRAKPQSLAELEAEYVNETLAAT
                     KGNKTEAARILGISRKNLYERLARQGKDGNREETQSGDKDEEVDL"
BASE COUNT          752 a          953 c          773 g          726 t
ORIGIN      
        1 ccttcccaga tggaccaggc ctttgcagct tccatgcgaa ccgcttcgtc atcactcatt
       61 aggcgcttgt gatatgcgcg caacaaatcg ccgcgttcag cctctggaat aatgcgcaca
      121 tactcttccc agatatccgg aaagaccgca ctagccccgt ggccctcctg gtaaaaccaa
      181 tcaatctcgt gcggacgaca aagaaaaatc ccccggagaa ttaatgcggt cactcgatcg
      241 ggatgagtct cggcataaga gagtgacaga gtgctccccc aggagccccc aaaaaccaac
      301 catcgatcga caccgagatg aaggcgaagg cgctcaatgt cagccacgag atcccacgtt
      361 gtattggcct ccagatttgc atgcggagtg ctctgccctg cgccacgctg atcgaaaaga
      421 acaatgcgat agttggccgg gtcgaagaac tgtcgatact cgggcacaat cccaccgcca
      481 gggccaccat gaaggaacac aacaggctct ccctttggat tgccacactg ctcgtagtag
      541 aggttatgaa cggatgacac ctggaggcgc ccagtgtcaa aaggctcaag ggccggatag
      601 agtgttctca tgacggcatc atacaaataa tcgtgacaga ttacgaaacg ctatgaattg
      661 caccaactag cgatgagctg ccggtccgtg ccagggtgag cacaaagact tcccttgcac
      721 cagcatcgag cagtgcactc ccgcaggccg aagcagttgc acccgtagta aaaacatcgt
      781 ccacaagtag gatgctctca tcagcaacga caagtggatg caaaactcta aacgcatcgg
      841 ccacgctggc tcgccttgcg acagcatcca ttcccgctcg gtgtcgttct gagtgttgag
      901 ttctgcaaag actcgcttga ctcatgggca ggccagtaag ttttgccact tcgtttccaa
      961 tcacaacggc ttgattgaat cctcgtgcct tttcgcgccg cggatgaaga gggacaggca
     1021 cgactctggt agcacgattc aaaggcgatc cttgctgtgt tagcaccaaa gcctcgcaaa
     1081 gcctgcgaca caagtgtggc tcttgtttca aggccagcac tgaagcccga agagcgccat
     1141 catagacacc gcaggcgcgt gctgctgaga acaggtcgtc ccggcagtcc tgacaatgga
     1201 cgcgatcact ctgtaggttg gccggtcgcc ccggcaagag cttcccacat ttcaggcaga
     1261 ccgcgtctgc ttcgcagatg aatttcactt tgctccaaca agagcgacag gcgatgccgt
     1321 cagcgcgagc ggatacctgt tctccgcaga cctgacacga ctgtgggtac aacaaggcca
     1381 gcgcggaatc gtagatagag ctaatggttg gcgtgaggcg catggcaaat ccgtgaggcg
     1441 ggttttagca aaaaagaacg gcctcctgac ccggttgtca gaaggccgca attatcccag
     1501 tcgaacctta aaacttctag tcctcctcgt cggtcagcag gccccgctcc tggaggtcct
     1561 gacaacgaac acaatggcgt gcccagggta ctgcttccag tcgtttttcc gtcaaggcct
     1621 cctcacagcg gacacagtgg ccatattcgc catcctcgat tcgctccagc gcttcatcga
     1681 tcaaatttaa tagctgccgg tcgttatccg acattgatac cagcagttcc ttggtgtagg
     1741 cgttggcggc catatctgcc ggatcctgtg tcgcttctgc atcgcgttca cgactataca
     1801 tctctgcctg ttggacctga ccaaccaatc cttcacgacg agccaaaagc ttgtcgcgaa
     1861 atcccttaac ttttcttttg tccattattt gttgctccta taaccctact tgctccaacg
     1921 ctacgaaaaa tttcgattgc ccttgagaat cacttagcgt atggcaaccg gtgaataatg
     1981 gcgtaggctc gatacaattg ttcgagcaag agtactcggg ccatctcatg ggttagcgtc
     2041 aaacgcgaca aagaccaacg tttatcaacc cggactttaa gctcctcaga aactccgtcg
     2101 ggtcccccta caatgaaagc cacagctttg gtgccaccgg tttcccactt ctgaatttgc
     2161 cgcgccagct cctctgaact ccattctctt cccgccgggt caagcaaaac ggtgacctca
     2221 tcatctaaaa gagcgtctga gatgctgcgc gactccctat cgatgcccgg cgatgttgcg
     2281 gctctcgtat ttgccctcag ttcagtagct tcgcagcgcg cgaaatgcga aagccgctga
     2341 aagtaatcgt cctgcaatgc ccttaagtgg gcgttccttg ttttgccggg ccaaaccaaa
     2401 tgaacgcgca ttacagatcc acttcctcat ccttgtcgcc agattgagtc tcttccctat
     2461 tcccatcctt tccttgccgg gctaaacgct catacaaatt cttgcggctt atgccgagaa
     2521 tgcgcgcggc ttcagttttg ttgcctttcg tcgctgccag tgtttcatta acgtattcgg
     2581 cctcaagctc agccaacgat tgcggctttg cacgtctccg ctgaagttgg accgccgcac
     2641 gaatggcctc agggagatga cccacctgaa tccgggagcc ggtacaaaca atcaccgccc
     2701 gctcgatagt gttgctgagt tcgcgaatgt tgccgggaaa ctcatactct tgcagcagtg
     2761 acattgcttc tggcgccaaa gccataacct cacgaccatg tttacctgcg tacgacaaaa
     2821 caaaatgttc gatcaacttc ggcaagtcct ctggcctttc acgaagtggt ggcacatgaa
     2881 tgtgaaccac attcagccga tagaacaggt cctcgcgaaa gttttttctt tgcaccgcct
     2941 gtactaaatt cacattcgtt aatgcgatca gtcgcgcatc taccccaatg gttcgcctgc
     3001 cgcccagtcg ctcaaattct cgagtttcga tcactcgcaa aagcttggct tgtgaatccg
     3061 tcgacaggta agcaatctcg tccaacacca gcgtcccttt atgagccgct tccaacctac
     3121 ccggcttggc ctccatggcg ctggtaaaag caccgcgttc atagccaaac agttcagcct
     3181 ccagcaggtc gctgggcagc gtcg
//
LOCUS       DASUEX010000604         2691 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1017898, whole genome shotgun sequence.
ACCESSION   DASUEX010000604 DASUEX010000000
VERSION     DASUEX010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2691
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1017898"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="VFV61_10030"
     CDS             <1..219
                     /locus_tag="VFV61_10030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900860.1"
                     /translation="VGCDADLIIWSPKKTFTIEPRAIYHRHKLTPYANRKFAGVVETT
                     FLRGRKIYDAGEFVGTPQGHLLLSEKTV"
     gene            231..1226
                     /gene="alc"
                     /locus_tag="VFV61_10035"
     CDS             231..1226
                     /gene="alc"
                     /locus_tag="VFV61_10035"
                     /EC_number="3.5.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR02961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004037 - allantoicase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoicase"
                     /protein_id="HEX4900861.1"
                     /translation="MEFTELIDLAAEKLGGAVLFANDEFFAPKENLLKASAPIFIEGK
                     YTDLGKWMDGWESRRRRAPGFDWCLVRLGLPGKVRGVTVDTSHFKGNYPEQCSLEAVD
                     LPGQPTISDLTNESLAWAEILPVTALQGDSQNHFAILSDDRTTHLRFKIYPDGGVARL
                     RVYGEVMPDWDRLRRLGGPVDLAAAEHGGLVLSCSDMFFGHRHNLIMPGRAANMSDGW
                     ETKRRRGPGHDWSIIKLGAAGQISHLEVDTAYFKGNFPESCSLEAINSASLPDEDVSG
                     AAVSWKTILPRTKLLAHTRHFFETELQNAGTVSHVRFNIFPDGGVSRLRVYGKPA"
     gene            1210..1743
                     /gene="uraD"
                     /locus_tag="VFV61_10040"
     CDS             1210..1743
                     /gene="uraD"
                     /locus_tag="VFV61_10040"
                     /EC_number="4.1.1.97"
                     /inference="COORDINATES: protein motif:HMM:NF010372.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline
                     decarboxylase"
                     /protein_id="HEX4900862.1"
                     /translation="MENLPNGLSQLNLLQPTAAETEFLKCCGSRKWAQQMSRRLPFRD
                     ERDLFETAEQVWWTLSPEDWLEAFRSHPKIGEQKPGTATSASALSWSRKEQQGVADAS
                     ANTRELLAKLNQRYEEKFGFIYIVCATGKSSEELLTILQERLANESETELRNAAKEQA
                     QITRLRLNKLLASWEQS"
     gene            1751..2095
                     /gene="uraH"
                     /locus_tag="VFV61_10045"
     CDS             1751..2095
                     /gene="uraH"
                     /locus_tag="VFV61_10045"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR02962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HEX4900863.1"
                     /translation="MSTISTHILDTSRGKPANGVAVYLESQNHDESWTQLTHAWTDDE
                     GRVKPFFLLEEPLPAGTYRLVFDTEAYFSALEAECFYPQVCVVFQIGEDPQHYHIPLL
                     ISPFGYSTYRGT"
     gene            2197..>2691
                     /locus_tag="VFV61_10050"
     CDS             2197..>2691
                     /locus_tag="VFV61_10050"
                     /inference="COORDINATES: protein motif:HMM:NF017945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HEX4900864.1"
                     /translation="MIDPTLSEWLNLITRWIHVFAGIMWVGTTYYFTWLDARLTEEEK
                     AVANTGSAAQIWMVHSGGFYVVEKRKIPDELSRKLHWFRWEAGTTWLSGMALLIIVFY
                     LGGGALVDPDVSSISVNAAIALGVGVLVVGWIVYDLLMLSPLRKNEKLFAVLAYLIIV
                     AVAYG"
BASE COUNT          630 a          684 c          755 g          622 t
ORIGIN      
        1 gtcggttgcg atgcggactt gatcatctgg agtcctaaaa agacattcac cattgagccc
       61 cgggcaattt atcatcgcca caaattgacg ccctacgcga accggaaatt tgccggcgta
      121 gtggaaacaa ctttccttcg cgggcggaag atatatgatg ccggcgagtt tgttgggacg
      181 ccgcaaggtc acttgttgct gagcgagaag acagtttgag aagcctaact atggaattca
      241 cagaactcat cgacctcgct gcggaaaaac tgggtggcgc cgtcttgttc gccaacgacg
      301 aattttttgc gccaaaggaa aatctgctaa aggcgagtgc gccgattttc atcgaaggaa
      361 aatacaccga cctgggcaag tggatggacg gttgggagtc gcgccgccgg cgcgccccgg
      421 gctttgattg gtgccttgtg cggctgggac ttcccggcaa ggttcgcggc gtgacagtag
      481 atacgagtca cttcaaaggt aactatccgg agcagtgctc gcttgaagca gttgaccttc
      541 cgggccagcc aacaatctct gatctaacta acgagtcgct cgcctgggcg gagattcttc
      601 ccgtaacggc tctgcagggc gactcgcaga accattttgc gatcctcagc gacgaccgaa
      661 ctactcatct tcgtttcaag atctaccccg atggcggcgt tgcccgtttg cgagtctatg
      721 gggaggtgat gcctgattgg gatcgcctga ggcgcctcgg tgggccggtc gatctcgcgg
      781 ccgcggaaca cggcggactc gtgctctctt gcagcgacat gttttttgga catcgacaca
      841 acctgatcat gcctggtcgc gccgcgaata tgagtgatgg ctgggaaacc aagcgacgcc
      901 gcggtcccgg tcatgattgg tcgatcatta aactgggtgc cgccggtcaa atcagccatc
      961 tggaagtcga taccgcctac ttcaaaggta actttccgga aagctgttcc cttgaagcta
     1021 ttaactcagc gagtttgccg gatgaggatg tgagcggagc agcagtctcc tggaaaacga
     1081 tcttaccgcg cactaagcta ttggctcaca cgcgccactt cttcgaaact gaacttcaga
     1141 atgcgggcac cgtctcacat gttcgtttca acatcttccc cgatggtggt gtcagccggt
     1201 tgcgcgtgta tggaaaacct gcctaacggc ctgagccaat tgaatctgtt acagccgaca
     1261 gcggcagaga ctgaattcct caagtgctgt ggctcaagaa agtgggcgca acagatgagc
     1321 cggcgtcttc cttttcgcga tgagagagat ttgtttgaaa ctgcggagca agtctggtgg
     1381 acactgtcgc ctgaagattg gctcgaagct tttcgcagtc atcctaagat tggcgagcaa
     1441 aagccaggaa ctgcaacctc agcctcggca ctttcatggt cgcgtaagga acaacagggc
     1501 gttgccgatg ccagcgccaa caccagggag ttgttggcta aactcaatca gcgctatgaa
     1561 gaaaagtttg gtttcatcta tattgtctgc gccaccggta agagttcaga agaattgttg
     1621 acgatcctcc aggaacgtct ggcgaacgaa tcagaaactg aattacggaa cgctgcgaag
     1681 gagcaagccc agattaccag gctgaggctt aacaagctgc tagcaagttg ggaacaatcg
     1741 tgaacgaaca atgagcacaa tttcaacgca catacttgat acgtcccgcg gtaaaccggc
     1801 aaatggagtt gctgtttatc tggagagcca gaaccacgat gagagctgga cgcagttgac
     1861 tcatgcctgg accgacgacg aaggacgagt gaaacccttc tttctcctcg aggaaccctt
     1921 gccggcggga acttaccgcc tggtgttcga caccgaagct tatttttcag ccctggaagc
     1981 cgagtgtttc tatccccagg tttgtgtggt gttccaaatt ggagaggacc cgcagcacta
     2041 tcacattccg cttttgatca gtcctttcgg ttactcgact taccggggaa cctagccaac
     2101 gaagtagtct caagactcag gtctcaagac tcaagaagac ctttcagacc tgaaacttga
     2161 aacctgagac ctgagacttg agacctgaga cttcaaatga tcgatcccac gctcagtgaa
     2221 tggctcaacc tgatcacgcg ctggatccat gtgtttgccg ggatcatgtg ggtgggcacc
     2281 acctactact tcacctggct ggatgcgcgt cttactgaag aagaaaaagc ggtcgctaat
     2341 acaggttcag ccgcgcagat ctggatggtg cacagtggcg gcttctatgt cgtcgagaaa
     2401 cgaaagatac cggatgagct ctcgcgtaaa ctgcattggt ttcgttggga ggcaggaacc
     2461 acctggttaa gcgggatggc cttgctaatc attgtcttct atctcggtgg cggggcgctg
     2521 gtcgacccgg acgtcagtag cattagtgtg aacgcggcga ttgccttggg agttggagtg
     2581 ttggtagttg gctggattgt gtatgacctg ctgatgcttt cgccgttaag gaagaatgaa
     2641 aaactcttcg cagtccttgc ttacctgatt atcgtggcgg tcgcgtatgg c
//
LOCUS       DASUEX010000605         3438 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_791020, whole genome shotgun sequence.
ACCESSION   DASUEX010000605 DASUEX010000000
VERSION     DASUEX010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3438
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_791020"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1621
                     /locus_tag="VFV61_10055"
     CDS             <1..1621
                     /locus_tag="VFV61_10055"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HEX4900865.1"
                     /translation="TWGPTTAEVNRVKAMGFLAYLNEQFNAPVTNPAKGSNYPDLVFP
                     LDDQATACPTSSPDPNYNQTVCNRDNFSLYPVQRTFFSNALYGQDQLRQRVAFALHQI
                     LVVSGASPLNRSSWMTMYLQALDRGAFGNYRTLLQEVTLNPSMGEYLDMRLSTRTNPN
                     ENFAREILQLFSIGVNQLNLDGTPKVDSQGVPIPTYSQTTVNEFTRVFTGWNLAAPIG
                     PGINNWRDPMVPRGGTTHDVAAKTLLNGVVVPACGGTNAVCAQSDLNAALDNIFNHPN
                     VGPFVGKQLIQHLVTSNPSPAYVSRVARVFNNDCDSLYPIAGCSNARGNMKAVVQAIL
                     LDPEARGDVKTDPNYGKLREPAQYIINVLRALNVKSFDKTSTSDGVLGGRSSTDFTGT
                     LDQVIFSPPTVFSYYQPGYEVPGTKILGPAFGILSTSTTLRRANNINSLVYAGVTANT
                     TPTALSPDRPRGTSLDLSNLEALSANPGDIADLLNALMLHGTMSTQMRTSLINAMNAI
                     TTADGFGSVAQKRARVAAYLVATSSQYDIQR"
     gene            1627..3027
                     /locus_tag="VFV61_10060"
     CDS             1627..3027
                     /locus_tag="VFV61_10060"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX4900866.1"
                     /translation="MATSRRDFLRTSACALGSMALASSVESFGIVHALTPQAASDYKA
                     LVCIFLNGGNDGNNLLVSIDQYAGYSQARTASTLAIPQASLLPISPLGGGSYGLNPSM
                     PEMQSLFNQGRLAIVCNSGPLVEPLSRTTYQNGTGKRPLQLFSHSDQVGLFQTAIANE
                     VSQTGWSGRVADKTSVLNGLATFPQNISIAGINLLLTGVDTRQLAVADSNTTLANVLQ
                     LNMTGPAGDVSARTASFNQLRTLDTNFKLIKAASDTRTSAMQTDSALGAVNPQLVTPF
                     PNPSTSISRQLLQVARLIKASTDPATGINMKRQIFFCQIGGFDTHSNQRVNGQDLLLL
                     QLSQAMNAFYQATVELGVQNNVTTFTLSDFSRTLQPAGTGAAVGSDHAWGNHHLVMGG
                     AVLGNRLYGTFPTIQLNGPDDTDSGSGARGRWIPTTSVEQYAATLATWYGLSTSDLAA
                     VFPLIGRFSSPNLGFV"
     gene            3135..>3438
                     /locus_tag="VFV61_10065"
     CDS             3135..>3438
                     /locus_tag="VFV61_10065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor V domain-containing protein"
                     /protein_id="HEX4900867.1"
                     /translation="MNLSRPALEKLRLLLLPALLTVVLGFLLTDNPGTHSVHAFSAGP
                     PAGYTGAPNEEPEACAECHVPPGAGTGQISIAAPQTYIPGQTYQVTVTHTNSDPTRL"
BASE COUNT          789 a          988 c          880 g          781 t
ORIGIN      
        1 aacctgggga ccgacaacag ctgaagtgaa tcgagtgaaa gcgatgggct ttttggccta
       61 tctgaacgaa cagttcaatg cgccggtaac gaatccggcg aagggttcca actaccctga
      121 tctggttttt ccactcgacg atcaggcgac tgcatgtcct accagtagtc cggatcccaa
      181 ctacaaccag accgtctgta accgcgacaa cttctcactg tatccggtgc aacggacgtt
      241 tttctccaac gcgctttacg gccaggatca actgagacag cgtgtcgcat ttgccctgca
      301 ccagatcctt gttgtttcag gtgcttcgcc tctcaatcga tccagctgga tgacgatgta
      361 cctgcaggcg ctggatcgcg gcgcctttgg caactaccgc acgcttctgc aggaggtcac
      421 gcttaacccg tcgatgggcg aatacctgga catgcggctg agtacaagga cgaatccgaa
      481 cgaaaatttc gcacgtgaaa tcctgcagct tttctcaatc ggtgtgaacc agctcaatct
      541 ggacgggact cccaaagttg attcgcaggg cgtaccaatc ccgacttact cacagactac
      601 agtcaacgaa ttcacgcgtg tctttaccgg gtggaacctt gccgcaccga tcggtcccgg
      661 aataaacaat tggcgcgatc cgatggtccc tcgtggtggt actacgcatg acgtagctgc
      721 caagaccctg ctgaacggcg tggtcgttcc cgcctgtggc ggcaccaacg cagtttgtgc
      781 ccagtcagat ttgaatgctg cgctggataa tattttcaac catcccaatg ttggtccgtt
      841 cgtcggaaag caactgattc agcatctggt cacgagtaac ccgagcccgg catatgtgtc
      901 acgcgttgct cgcgttttca acaacgattg tgactcgctt tatccaattg ccggctgctc
      961 aaatgcgcgc ggaaacatga aggctgtcgt gcaagccatc ctgcttgatc cagaggcacg
     1021 gggcgacgtt aagaccgatc cgaattatgg caagctgaga gagcccgccc agtacatcat
     1081 taacgtgctg cgtgcactca acgttaaatc cttcgacaaa acgtcaacca gtgatggtgt
     1141 tttgggtggc aggtcgtcta ctgatttcac aggcacattg gatcaggtaa tcttttctcc
     1201 tccgacagtg ttcagctatt accaacccgg atacgaagtt cccggcacca aaatccttgg
     1261 tccagccttc gggatactct cgacgtcaac cacactgcgt cgtgctaaca acatcaactc
     1321 attggtctac gccggcgtaa cggccaacac cacacccacg gcgttgagcc cggaccgccc
     1381 ccgtggaact tcactcgacc tctcgaacct tgaggcgcta tctgccaatc ccggtgatat
     1441 agccgacctg ctgaacgccc tgatgcttca cggaacgatg tccacacaaa tgcggacttc
     1501 ccttatcaac gcgatgaatg ccatcacgac tgccgacggg tttggctcgg tcgcgcagaa
     1561 gcgagcgcgc gtggccgcct acctggtggc aacttcatcg cagtacgaca tacagagata
     1621 aacacaatgg ctacatcacg cagagatttt ctccgaacca gcgcctgtgc gctcggcagc
     1681 atggcgttgg cttcgagtgt cgaaagcttt ggcatcgttc atgcactaac gccacaggcc
     1741 gccagtgact acaaagcgct ggtctgcatc ttcctgaatg gcgggaatga tggaaacaac
     1801 ctgctggttt cgattgacca gtatgccggc tactcacaag ctcgaaccgc ctccacgctt
     1861 gcgattccgc aagccagcct gctgcccatc tctccactcg gtggaggctc ttacggcttg
     1921 aacccgagca tgccagagat gcagtcgctc tttaatcaag ggcggcttgc tatcgtctgc
     1981 aacagcggac cactcgttga acccctctct cgtacgacct atcaaaatgg gactggcaag
     2041 cgtccattac aacttttctc ccattcggat caggtcggac tatttcagac cgcgatcgca
     2101 aacgaagtca gccaaacagg atggagtggc agagttgccg ataagacttc ggtcttgaat
     2161 ggcctggcaa cttttccgca aaacatctcg atcgccggaa taaatctgct gctcaccggc
     2221 gtcgacacac gccagctcgc agtcgcagat tcgaatacga cactagcaaa tgtgctgcaa
     2281 ctgaatatga ctggaccagc gggcgatgtg agtgcccgca ccgcttcatt caatcaattg
     2341 cgcactttgg acaccaattt caaactcatc aaagctgcca gcgatacgcg gacgagtgcg
     2401 atgcaaactg acagcgcatt gggtgccgtc aatccgcagc tagtgacacc tttccccaat
     2461 ccgagtactt cgatcagtcg ccaactgttg caggtcgcaa gattgatcaa ggccagcaca
     2521 gatccggcca ccgggatcaa catgaaacgg cagattttct tttgccagat cggtgggttt
     2581 gatactcatt caaatcaacg tgtaaacgga caggatttgc tgctcctaca actcagtcag
     2641 gcaatgaatg cgttctacca ggccacggtc gagctgggag tgcagaacaa tgtcacgacg
     2701 ttcacgctct ctgacttttc gcgcacgctg caaccggccg gaacgggcgc agccgtgggt
     2761 agcgatcacg cctggggcaa tcatcacctg gtaatgggtg gtgcggtgct gggaaaccgc
     2821 ttgtacggca cgtttcccac gattcagctt aatggaccgg atgataccga cagcggaagt
     2881 ggtgcccggg gacgatggat tccgactact tcagtcgagc agtatgcggc tacgcttgcc
     2941 acctggtatg gcttgtcaac cagcgatttg gcggctgtgt tcccgttgat tgggcggttc
     3001 tcgtcgccaa acctcggttt tgtatagaaa ccaggtcgaa agctgtctgg tggtttcgga
     3061 aatttagata gcctagcgaa attcgctagg ctatttttca gcaggtatct tttcgtaagg
     3121 gattgtctcc agtgatgaat ctctccaggc cagcactcga aaaactaaga cttcttcttc
     3181 tgcccgcact gctgacagtt gtgcttggtt ttttgttgac ggataaccca gggacgcatt
     3241 cagtccacgc cttctctgca ggaccgccgg caggttacac gggtgctccg aatgaagagc
     3301 ctgaggcgtg tgctgaatgt catgtaccgc caggcgccgg caccggtcag atttcaattg
     3361 ctgcgccgca gacatatatt cctggtcaga cttaccaggt gacagtaacc cataccaatt
     3421 ccgatccgac gcgcttgc
//
LOCUS       DASUEX010000606         4651 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_444150, whole genome shotgun sequence.
ACCESSION   DASUEX010000606 DASUEX010000000
VERSION     DASUEX010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4651
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444150"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(510..3614)
                     /locus_tag="VFV61_10070"
     CDS             complement(510..3614)
                     /locus_tag="VFV61_10070"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX4900868.1"
                     /translation="MSLKRVANILALCLCLTGIAFGQETTGTIEGTVVDAQGGRIAGA
                     TVLVEGNAFNRTVTTDSDGFYRILQVPPGTYSITTSATNFASDKIQDVQVTLGRVTTT
                     EIKLNAGDVSAAVVVTDADVAAIDTSSNKIQTNITTEVINSTPKGTRVDSVLQLSGAT
                     RNEPLSRGYQIDGSSGAENTFVIDGSEVTNFRTGQLRDTNNMPFQFVQEVQVKTSGFD
                     AEFGGATGGVINVVTKSGGNQFHGEFGAQFETSNLSARQTLTGKVANSDPQYQANPLI
                     QGTSASVLRYINPPGDSFFNTYPSITLSGPIVKNRLWFLVSAAPQFYSAKRDFLFPTG
                     QTVHYERNLRNDYEFARLDSSVTDNLRLSGTFTYSPQREHGQIPSYTTSAPPSNDFSQ
                     LGGRVAANNVTGSGVWTPTSKLVISGRFGRNYLNEKDSSYGVPAGPRIRCLNGGSPAG
                     VPATNPCASGFNSTSDITKTVKDISIRKTFEADASYLADNLAGRHAFKFGYQLNKLSN
                     DVDQGYFGVGEVRLFWGQSDRGIGPGVCPAGPCLSYGYGYLQDFGTVGLASSTNSAIY
                     VQDSWQPTARLTFNLGLRLEKEDVPSFSENGQAIKFGWGSKLAPRLGVAYDLLGNGKL
                     KVFASFGRFYDRFKYELPRGSFGGDKFLRYFFPLADPNYTTYTRAYALSHTLVGPLDF
                     RVPSNDPADNRVDPDLKAARQTEFTVGAEYGLSNNLVLSGRYTRKNLDRTIEDVGFID
                     ALGNENFFIANPGMGIVSQPFAPGLPASPLAKRLYNAMELRLDKRFSQNYFFNVSYTF
                     SRLTGNYSGLSSSDEPDGLGVGRSSPNVNRFFDLPFLGFNTNGEPDNGLLATDRPHAL
                     KVFGSYTFNWKDFLGGLDSKGANTTEIGASFIGLSGTPLSSRVQLYGAYTFLNTRGDL
                     GRTPMFTQTDFQLTHTIKLAGDGRYALKFDVNLLNAFNENNVLSVFSQISPDSLTAGN
                     FGISDADGLGELNTFRAVFAGGLTSQILAGLANGSIARDVRFNQPLTRQIPRQVRFGF
                     RFVF"
     gene            complement(3981..>4651)
                     /gene="mtnC"
                     /locus_tag="VFV61_10075"
     CDS             complement(3981..>4651)
                     /gene="mtnC"
                     /locus_tag="VFV61_10075"
                     /EC_number="3.1.3.77"
                     /inference="COORDINATES: protein motif:HMM:TIGR01691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acireductone synthase"
                     /protein_id="HEX4900869.1"
                     /translation="DILFPFARTHVRDYLIQHATTAEVEKDTQALFREYSIDQQKGKQ
                     PPQIETGVWSLDSIIAYVNWLIERDRKSSALKSLQGKIWEQGYRDGSLQAPLFVDVVP
                     NLQRLRRDGIGIAIFSSGSVLAQKLLFSHTETGNHSDLIDHYFDTAVGRKVEAASYGK
                     IAQRLALSPKETVFVSDVTEELKAAREAGMATLLCVRPGNPPQTTTEEFEVIHSFSQI
                     LPSE"
BASE COUNT         1111 a         1191 c         1297 g         1052 t
ORIGIN      
        1 ctccttttta atcagctcaa gagattcaaa gaggctgccg acgagttgga actatttttg
       61 aaagttcaac cagatgcgag agacgcagac aagatcaaga aactcatcca gcgtctaaga
      121 caaaagggct cttaaccagc ccgattgagg atcgaaccct tcctcagctt tgctcccata
      181 ctcaaccgca acgaggtcac ggctatgccg ccggatgtga ggcgtggctc tgccgcgccg
      241 tggcgtcgcc tttgataaat tggggctatg ccgtaggtac ggcttacccc taaaaagtct
      301 gcgggcagag cccgatccac gggcgcagcc cgagttctaa tctaggtcct acggcgcggc
      361 aaagccgcgc ctcacatccg gcggcaaagc cgaaacgcat actgcgcatg gacttacgag
      421 gcgaacaaga gattgatcct caaccaaaaa agaagcccat ccaaaaagct cggatgggct
      481 tcgagggtca gttgttgaaa gtcgccggct tagaaaacga accggaaacc gaaacgaacc
      541 tgacgcggaa tctgtcgggt gagcggctga ttaaaccgca catctctggc gatgctgcca
      601 ttcgccaatc cggccaggat ctggctggtg agtccgcccg cgaaaactgc acggaaggtg
      661 ttcagttcac cgaggccatc cgcgtcgctg atcccaaaat tccctgccgt caaggaatcc
      721 ggggagatct gactaaacac gctgaggacg ttgttctcgt tgaaagcatt gagtagattg
      781 acgtcaaact tcaacgcgta gcgtccgtca ccagccaact tgatagtgtg agtcagctgg
      841 aaatcggttt gcgtgaacat cggggtgcgg cctagatctc cacgcgtgtt caggaaggta
      901 taagcaccat agagctgcac gcgactcgac agcggcgtac cactcaagcc gatgaacgag
      961 gcgcctatct cggtggtgtt ggcgcccttg ctgtccaaac caccaaggaa gtccttccag
     1021 ttaaacgtgt aggaaccgaa caccttcaaa gcgtgtgggc gatcggtcgc cagtaggccg
     1081 ttatccggct cgccgttggt gttgaagccg aggaatggca aatcgaagaa gcgatttaca
     1141 ttcgggcttg aacgaccgac gccaaggcca tcaggctcat ccgagctgga aagacctgag
     1201 tagttaccgg tcaaacggct gaaggtgtaa ctgacgttga agaaatagtt ctgcgagaag
     1261 cgcttgtcga gtcgcagttc cattgcgttg tataggcgct tggccaacgg cgacgccgga
     1321 agacccggag caaaaggctg cgagacgatg cccatgcccg gattggcgat gaaaaagttc
     1381 tcattgccga gagcgtcgat gaacccaacg tcctcgatcg tgcggtcaag gttcttacga
     1441 gtgtagcgac cgctcaatac gaggttgtta ctcaatccgt attcggcgcc aacagtaaat
     1501 tcagtctgtc gtgccgcttt tagatccgga tcgacgcgat tgtcagccgg gtcgttggaa
     1561 ggaacgcgga aatcaagcgg accaaccaga gtgtgtgaca gagcgtaggc tctcgtgtaa
     1621 gtcgtatagt ttggatcagc cagcgggaag aagtagcgca ggaacttatc gcctccgaat
     1681 gagccacgtg gaagctcgta cttaaagcgg tcatagaacc tgccgaaact ggcgaagacc
     1741 ttcagtttgc cattgccgag caggtcgtaa gccacaccta accgtggcgc caatttagat
     1801 ccccaaccaa attttatcgc ttggccattc tcggagaagg agggaacgtc ttccttttcc
     1861 agacgcagac cgaggttgaa ggtcaaacgg gcggtaggct gccagctgtc ctgaacatag
     1921 attgcgctgt tcgtgctgct cgccaaaccg acggttccga agtcctgaag gtagccgtaa
     1981 ccataggaaa ggcacgggcc agcaggacaa actcccggtc caataccgcg atcggactga
     2041 ccccagaaca agcgaacttc accaacaccg aagtagccct ggtctacatc gttggagagc
     2101 ttgttgagct ggtagccaaa cttgaaggcg tgacggcccg caaggttatc cgccaggtaa
     2161 ctggcatccg cttcaaacgt cttgcggatc gaaatatcct taacggtctt ggtaatgtcc
     2221 gacgtgctgt tgaacccgga agcgcacggg ttggttgcgg gcacgccagc tgggctgccg
     2281 ccgttgagac accggatgcg tggtccagca ggaacgccat acgagctatc tttttcgttc
     2341 aaatagttgc gaccgaagcg cccactaatg acaagttttg atgtgggcgt ccagacgcca
     2401 ctaccagtga cgttatttgc tgcaacccgg ccacctaact ggctaaagtc attcgacgga
     2461 ggtgccgagg ttgtgtagga aggaatctgt ccatgttcac gttgcggact gtaagtgaaa
     2521 gtgccggata ggcgcaggtt gtccgtgaca gacgagtcga gacgtgcgaa ctcatagtcg
     2581 ttcctgaggt ttcgctcgta gtggactgtt tgcccagtgg gaaagagaaa gtcgcgcttg
     2641 gcgctgtaaa actgtggggc cgcgctgacc aggaaccata gcctgttctt tacgattggc
     2701 ccactgagcg tgatgctggg atacgtatta aagaaagagt cgcccggtgg gttgatatac
     2761 ctcagaaccg acgcgctggt tccctgaatg agcggattgg cctggtactg tggatcactg
     2821 ttggcaactt ttcctgtcag ggtttggcgc gcgctgagat tgctcgtttc aaactgcgct
     2881 ccgaattccc cgtgaaactg attgccgcca ctcttggtga ctacattgat cacaccaccc
     2941 gttgcgccgc caaactcggc atcgaaacca ctcgtcttta cttgcacttc ctgtacgaat
     3001 tggaaaggca tgttgttggt gtcgcgaagt tggccggtgc ggaagtttgt aacttcagaa
     3061 ccgtcgatca cgaaggtatt ttcggcgccg cttgagccgt cgatttgata gccacgactc
     3121 aatggttcat tcctggttgc accagagagc tgcaacacgg aatcaaccct ggtccccttc
     3181 ggagttgaat tgatgacctc ggttgtgatg tttgtttgga tcttgtttga tgaagtgtcg
     3241 atggcggcca cgtctgcgtc tgtgacgacc acagcagccg aaacgtcacc agcgtttaat
     3301 ttgatctctg tggtggtaac cctgccaagt gtgacttgaa cgtcctgaat tttgtcgctg
     3361 gcaaagttgg tggcgctggt tgtgatcgaa taggttcccg ggggaacttg caatattcga
     3421 taaaaaccat cgctgtcggt agttactgtg cggttgaagg cattgccttc aacaagcact
     3481 gtggctccgg caattcggcc tccttgagcg tcaaccaccg taccttcaat ggtgccggtt
     3541 gtttcctgac caaaagcaat gccggtgagg caaagacaaa gggccagaat gttcgcaact
     3601 cgtttaagac tcatttttac tttctccttc cgatctaaaa cggacatttg ctaagtagct
     3661 cgcttaccgg ggaagtgtcg tcgctaaacc ttcgctccga aaaactggag taccgcaagg
     3721 cagcggtaac actgaaggtg caatgaccgt gcccggcgcg ggaggatcgt ctcaggcccg
     3781 ttcgtccctc gttcaagccg actcgtactg caaaccgaag aaatggagtt gcggaaaatg
     3841 gattggacta caggaaggtt gcagttaatg gattagatag ttagagagcg gttcgcttgg
     3901 cagcgcggat tacagatttt cggagaactg atcacggcga tcaaaacaga gacattaaca
     3961 agggtggagc aagagagttt tcactcgctc gggaggattt gcgaaaagct atgtatgacc
     4021 tcaaactcct cggtcgtagt ttgtggcggg ttgcccggtc tgacacaaag tagagttgcc
     4081 atgccagctt cgcgcgcagc cttgagttcc tctgttacat cagaaacaaa aacagtttct
     4141 tttggtgaga gcgcgagacg ttgcgcgatc ttgccataac tcgccgcctc aactttgcgt
     4201 cccacggctg tatcgaagta atggtcgatt agatcggagt ggttacccgt ttcggtatgc
     4261 gaaaacaaga gcttttgtgc aagcacgctg cccgaggaga agattgctat tccgatgccg
     4321 tccctgcgca agcgctgcaa gttcggaacg acatcaacaa acaagggtgc ctgcaaactg
     4381 ccatcacgat agccctgttc ccaaatcttc ccttgcagcg actttaaggc tgatgatttg
     4441 cggtcccgct caatcagcca gttaacgtaa gcgattatcg aatcaagtga ccagacccca
     4501 gtctcgattt gcggcggctg tttacctttc tgctggtcaa tagagtattc gcgaaaaagt
     4561 gcctgggtat ctttctccac ctcggcggtg gttgcgtgtt gaatcaaata gtctcttacg
     4621 tgagttcgag caaaagggaa gaggatgtcg t
//
LOCUS       DASUEX010000607         4416 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_107766, whole genome shotgun sequence.
ACCESSION   DASUEX010000607 DASUEX010000000
VERSION     DASUEX010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4416
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107766"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            58..1440
                     /locus_tag="VFV61_10080"
     CDS             58..1440
                     /locus_tag="VFV61_10080"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX4900870.1"
                     /translation="MSTRHLQFAVLTLGSVLLLATSVASQTTAATSLSREVMTKTDER
                     PAQELYEDANGYLGRKYAEFNKQKLGYDAQLEAKTKQEQKALAVANAAILTRRKDLSA
                     DDLYYLGMLQHLVGDSDATLKVMRRYLAKNSQGDKSQIARAVVVLHATRTNLLPEAET
                     TVATYRKHEPIDFNELYGMETLLTEAFGKANDLARVEAHARAMLEVARNEKAIKKVSS
                     FKRDERLFKAASQLSEVLLKTDRHDEALAVWRDLVKLSIALPSGNLYKMARMRLASLD
                     PAADPLKLLSDKSNEKGVPPEIVGVQWIDQQPVKLAELRGQVVLLDFWAHWCGPCQYV
                     FPKLQRWHESYKDKGLVILGLTNYFGQTKGRRATPTEELAYLRDFKKRNRLPYGFVVS
                     DSQINDANYGVTSIPMSFLIDRNGTVRFIAVGANDQETTALGKKLKELIDEPAPSSAS
                     GAPRSAVQKQ"
     gene            1516..2742
                     /locus_tag="VFV61_10085"
     CDS             1516..2742
                     /locus_tag="VFV61_10085"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein/redoxin"
                     /protein_id="HEX4900871.1"
                     /translation="MEGSSITIGIAFLAGILSFLSPCVLPLVPGYVSLMSGVSIDRLK
                     EGGSASKARSAVIFNSLAFNAGLSVIFLALGATAGLVGATILNNFWVRVIGGVIIIAF
                     GLQLIGLLKISALYKDTRFFSDDKPRGMAGSFTLGIAFAAGWTPCIGPILGGIIGLAA
                     TSGGWRSGLLLSAFYSAGLAIPFLLTGLGINQFLGFYGKFRRHLHKVEVVSGVILIVV
                     GLLVASGFSTLLASSTLASILPNAESWLKVNPGAPVTTVAPENRGAFPQAPDVQLKTL
                     EGKAFQLAELRGRVVLLNFWATWCIPCRSEIPELNIMHRDLQARGLSVVGVSTYDGAD
                     GVRNFWKDIKQDYPVLLGDQNVESTFAVSALPTTFILDREGRIRAKIIGERRRETFEA
                     AVKPLLEETTTAKATK"
     gene            2774..3424
                     /locus_tag="VFV61_10090"
     CDS             2774..3424
                     /locus_tag="VFV61_10090"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX4900872.1"
                     /translation="MFKLLTLVFLLIGLAPVAAKAQHEYSPLEEKTVNYKNWTLTGLA
                     DEKPVDLRSLLAGKKLVMVVYFAPWCGNWRFEAPVAAKLYEKYKAQGFEVIGVSEYAS
                     RDDVKAFFGPSGPPYPVVSESETRDDKQKTPHYGYRQLTGDRRNWGSPWNIFLEPAKL
                     AKTGEVLAEKAWVVNGELIEEDVDKFISERLSATTATAIEPCKPEVTSQKSGVKKQ"
     gene            3501..>4416
                     /locus_tag="VFV61_10095"
     CDS             3501..>4416
                     /locus_tag="VFV61_10095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900873.1"
                     /translation="MNLRLLYHGHCFDGVASAAIFSRFYQEKVHPEAEIHYRGLLHRP
                     GTLFDLEMFDGDENAIVDFKYAASERLTWWFDHHESAFLTPEDEAHFRADTSGTKFVD
                     PSRKSCTEFIADIASSRFGFNPEPLHSLVHWAHIIDGALYESAAQCVELQEPALQLMQ
                     VIEADPDDNFIEELIRQLVNQPLEAVASSAEVKRRFRPIQQQHLVTVEAVRRKAVFAD
                     RVVRFDLVDEGYEGFNKFIPYYLYPETTYSVALTRGSVRTKISVGSNPWAPVPRRHNI
                     AKICERYGGGGHAVVGAISFRPDEVERGR"
BASE COUNT         1006 a         1117 c         1267 g         1026 t
ORIGIN      
        1 tcagactttt gctcctgacc gcttcgctgc ggtaggtatt aactaactcc agttcaaatg
       61 agcactcgcc atcttcaatt cgccgtactt accctgggct ccgtgttatt gcttgcgact
      121 tcagtggcca gtcaaaccac cgcggcaact tcgctctcaa gggaagtaat gactaagact
      181 gacgagcgcc cagctcagga actgtatgaa gatgcgaatg gttatctggg tcggaagtat
      241 gcagagttta ataaacaaaa gcttggctac gatgcgcagc tggaggcgaa aacgaagcag
      301 gagcaaaagg cgctcgctgt tgcgaacgcc gcgatcctca cccgtcgcaa agatctttct
      361 gcagacgatc tttattacct gggaatgctg cagcacctgg tgggcgattc agacgcaaca
      421 ctcaaagtga tgcggcgtta tctcgcaaag aattcccagg gagataagtc gcagatcgct
      481 cgggcagtcg ttgtcctgca tgcaacgcgc acgaacctgt tgccggaagc ggaaacgacg
      541 gttgctacct atcggaagca tgagccgata gacttcaatg aattgtacgg aatggaaacg
      601 ctgttaacgg aggctttcgg caaggcaaat gatttagcgc gcgtggaagc tcacgcgcgt
      661 gcgatgctgg aagtcgctcg caatgagaag gcgattaaga aagttagcag cttcaagcgt
      721 gatgagcgct tgttcaaagc cgcttcacag ctttccgaag tgttgctaaa aaccgatcgc
      781 cacgacgaag cgctcgctgt ctggcgcgac ttggtgaagc tctcgatcgc cttgccctca
      841 ggtaatctct acaagatggc gcggatgcgg ctggctagtt tggatcccgc ggctgatccg
      901 ctcaagttgc tatcggataa aagtaatgaa aagggtgtgc ccccggaaat tgttggcgtg
      961 cagtggatcg atcagcagcc ggtaaagctg gcggagttgc gtggccaggt ggtgctcttg
     1021 gatttttggg cgcactggtg cggaccctgc cagtatgttt tccccaaact gcaacgttgg
     1081 catgaaagct ataaggacaa aggcctggtg attttaggtt tgacgaatta cttcggtcag
     1141 acgaagggcc gccgcgctac gccaacggaa gagctagctt atctccgtga ttttaagaag
     1201 cgcaaccggc tgccctatgg attcgtggtg tctgattcac agataaacga tgctaattac
     1261 ggcgttacat cgattccgat gtcgtttctg attgatcgga atggcactgt gcgcttcatc
     1321 gcggtgggcg ctaacgatca ggaaacaacc gctttaggca agaagctgaa agagttaatt
     1381 gacgagcccg caccgtcatc agcttcagga gctccgcgga gcgccgtgca gaagcaatag
     1441 cccggtttcg ttgctgagag agtttgctct tataatcagg tcaaaatcgc ctggcgcaga
     1501 attcaggact aaataatgga aggttcaagc atcaccatcg gcattgcctt tttagcgggt
     1561 atcctctcgt ttctttcgcc ctgcgtgttg ccgctggtgc ccgggtatgt ttcgttgatg
     1621 tcgggcgtca gcattgaccg gctcaaggaa ggtgggtcgg cgtcaaaggc gcggagcgcg
     1681 gtgatcttta actcacttgc tttcaatgcc ggcctctcgg tgatctttct ggccctcggt
     1741 gctacagcag gactcgtcgg ggccacgatt ctgaataact tctgggtcag agtaataggc
     1801 ggcgtaatca tcatcgcctt cggactgcag ttgattggct tgcttaaaat ctccgcactc
     1861 tacaaagaca cgcgtttctt ttccgacgat aagcctcgcg gcatggccgg ttcgtttacg
     1921 ttgggaattg ctttcgcggc gggttggacg ccctgcatcg gaccaatatt gggaggcatc
     1981 atcggactcg cggcgacgag tggcggttgg cgtagtggac tcttactatc ggccttctac
     2041 tcagcggggc tggccatacc ttttctatta accggcctgg gcattaatca gttcctcggt
     2101 ttttacggaa aatttcgccg tcacctgcac aaggttgagg tggtttccgg agtcatcctg
     2161 attgtagtgg gtctacttgt agcctcgggt ttctcaacgc tgctggccag ttctacactc
     2221 gcgagtatct tgccgaatgc ggagagttgg ttgaaggtga acccgggcgc gccggtgact
     2281 acagtggccc ccgaaaatcg tggcgccttt cctcaagcac ccgatgtcca actgaagacg
     2341 ttggagggaa aggcctttca actggcagag ttgcggggac gcgtagtgtt gctgaatttc
     2401 tgggccacct ggtgtattcc ctgccgctca gaaattcccg aactgaacat catgcatcgt
     2461 gacttgcagg cccgcgggct gtcggtggtg ggcgtctcta cctatgacgg cgccgatgga
     2521 gtgagaaact tttggaagga catcaagcag gactaccccg ttctactcgg agatcagaat
     2581 gtggaatcca cgtttgctgt gagtgcgctg ccgactacct tcatcctgga tcgcgaaggt
     2641 cgcattcgcg cgaagataat cggcgagcgc cggcgcgaaa cgttcgaagc ggccgtcaag
     2701 cctttactgg aagagaccac gacggcaaaa gctactaagt gaagattccc aggtggagtt
     2761 aaggagtatc gcaatgttca aattgttgac gctggtgttt ttactaatcg gcttggcgcc
     2821 ggttgccgcc aaagcgcagc atgaatactc tcccctggaa gaaaagacgg tcaactataa
     2881 aaactggacg ctcacgggac ttgcggacga aaaaccggtg gatctgcgct cgctcttggc
     2941 gggcaagaaa ctcgtaatgg ttgtttattt tgccccctgg tgcggcaact ggcgttttga
     3001 agccccagtc gcggcgaagc tgtatgagaa gtacaaggcg cagggttttg aagtaatcgg
     3061 agtaagcgag tatgcatcgc gcgacgatgt gaaggctttc ttcggcccca gtggaccgcc
     3121 gtatccggta gtcagcgaat ctgagacgcg cgacgacaag cagaaaacgc cgcattatgg
     3181 ttatcggcag ttgactggcg acagacgaaa ctggggctcg ccgtggaaca tctttcttga
     3241 acccgcgaag ttggccaaaa ccggcgaggt gctagcggaa aaggcctggg tggttaatgg
     3301 tgagttgatt gaggaagatg tcgataaatt tatcagtgaa cgcttgagcg ccaccactgc
     3361 gacggcaatt gagccatgca agccagaagt cacgagtcag aagtcaggag tcaaaaagca
     3421 gtaggcagag gcaaagatcc aagttcaaag ttccaagttc aaagctccaa gtccaaagtc
     3481 caaagccccc agaccactta atgaaccttc gtctgctcta ccatggccat tgttttgatg
     3541 gagttgcatc ggccgccatc ttcagccgct tctaccagga aaaagttcac ccggaagcag
     3601 agatccatta ccgcggattg ctgcatcggc cgggaacgct tttcgatctg gaaatgtttg
     3661 atggtgacga gaatgcaatc gtcgatttca aatacgctgc ctctgaacgc ctgacctggt
     3721 ggttcgatca tcacgagtct gctttcctca cacccgaaga cgaggcccac ttccgtgccg
     3781 atacctcagg aacgaagttt gtcgatccca gtcgcaagtc gtgcacggag tttatcgcag
     3841 acatcgccag ttcgcgtttc ggcttcaatc ctgagccgct ccactcgtta gtgcactggg
     3901 cccacattat tgacggggcg ctttacgaat cagcggcgca gtgcgtcgaa ctgcaggaac
     3961 cagcgctgca attgatgcag gtgatcgaag ccgacccgga tgacaacttt attgaagagt
     4021 tgattcgcca actggttaac cagccactgg aggcagtcgc gtcgagcgct gaggtaaagc
     4081 gccgtttccg gcccatccag caacaacatt tagtgactgt ggaggcagtg cgacgcaaag
     4141 cggtctttgc tgatcgagtt gtgcgttttg atctcgtaga cgagggttac gaaggcttta
     4201 ataaattcat tccttactac ctctatccgg agacgactta ttcagtggcg ctcacgcgtg
     4261 gctcagtacg caccaagatt tcggttgggt ccaatccctg ggctccggtt ccgcgccggc
     4321 acaacatcgc gaagatctgc gaacgttacg gcggtggcgg acatgcggtg gtgggggcaa
     4381 tctcgtttcg cccggatgag gtagagcggg gccgaa
//
LOCUS       DASUEX010000608         5185 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1361664, whole genome shotgun sequence.
ACCESSION   DASUEX010000608 DASUEX010000000
VERSION     DASUEX010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5185
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1361664"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            229..1488
                     /locus_tag="VFV61_10100"
     CDS             229..1488
                     /locus_tag="VFV61_10100"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX4900874.1"
                     /translation="MKKSFAILVLLSLLTLSAFSASAQSRPRRVGQTTPTTAPNSPTT
                     NRAPVLGGTNTAGNQSGTAQPAPETGPEEVDAGDVIKVDTTLVTLPVSVTDRDGRYIP
                     NLTKSDFRLWEDGVEQQVAFFTSVDKPFSVVLVLDTSGSTRFKIEEIQDAAIAFVNQL
                     RTDDRVMIISFDDDIRVLTEFTGDRNRMRDAIMRTRTGNGTRLYDAVDLVINQRLNQV
                     DGRKAVVLFTDGVDTASRHASYASNVRDAEELDALIYPVQYDTFRDVGGGGGGGIGWP
                     GGRGSTNPIGILIDIWGRRGGRSGPIGGGGRGGVGGNRSDYERADRYLRELAQKTGAR
                     QYQADNTNNLSYAFRNIAEELRRQYSLGYYPIRPPQPGERRQVRVRVNQPNLAVRSRD
                     SYVFGQDGTVNSQKTAQTPVLRRKMTN"
     gene            complement(1485..3545)
                     /locus_tag="VFV61_10105"
     CDS             complement(1485..3545)
                     /locus_tag="VFV61_10105"
                     /inference="COORDINATES: protein motif:HMM:NF017081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HEX4900875.1"
                     /translation="MKFGSWRYFGLVGAVTLTYFLAAELGLSLASVHTNVSPVWPPTG
                     IAIAALLLFGRRLWPAVLIGAFLANLWTEVSTPVALGIAIGNTLEAVTASYLLSYRSP
                     FDYSFSSIRSVLKFVLFAVVFSSMISSTIGNISLCIGGEAQWANFGSLWLTWLLGDGF
                     GALIVAPFLLAWGTNLKWRTSPQAVIEAAILLATLFLVSLISFAGWIPGPDKRYPIAH
                     LTMPFLVWAALRLDQRIVTSATVLMSAVAVWGTAHGYGSFVRGGPNESLLLLQIFVGS
                     AALLTYVLYAVMCERKTAQKETLGLVAEVARQQKRVEDIVAHVPGVVWEAWGEPTADS
                     QRIDFVSNYVEKMLGYSVEEWLSTPNFWRTIVHEDDREAAGREAARTFASGTGGVNRF
                     RWLTKDGREIWVESQSVVVCDESGKPVAMRGVTMDITQTVRAQEERTRLLESEREARS
                     QAEQASRLKDEFLATISHELRTPLNAIVGWARLLRSGQLDPKNLEHAFEVIERNAWSQ
                     KQIIEDMLDVSRIITGKLRIGLVPLELTSVIQAAMDVVRPAAEAKEIKLKTRFNSPEL
                     LVSGDAERLQQVIWNLLSNAVKFTANGGLVEIVVQRKNGSAEIAIWDDGPGIPPDFLP
                     HVFERFSQADSSSTRRHGGLGLGLAITRHLVELHGGAVKAENREEGQGAILTVTLPAY
                     STSG"
     gene            complement(3789..4781)
                     /gene="galE"
                     /locus_tag="VFV61_10110"
     CDS             complement(3789..4781)
                     /gene="galE"
                     /locus_tag="VFV61_10110"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010991304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEX4900876.1"
                     /translation="MNVLVTGGAGYIGSVVVEELLCDGHEVTVYDNLSRGHREAVAPE
                     AEFAEGDLRDSERLRAVLRQHDVAAVVHMAAASLVGESVEKPGSYYENNVVAGLSLLD
                     AMRAEGVSRLVFSSTAAVYGEPPKQPIEEGDPTQPTNPYGETKLAFEQALKWYSHAYD
                     LRFTTLRYFNAAGATLRCGEWHSPESHLIPIVLQVAAGTRSNVELFGDDYPTRDGTCV
                     RDYIHVVDLARAHILALQSPRNALYNLGCGAGYSVKEVIDCAAQITGRAIPVKVTSRR
                     AGDPPTLIASSVAIKQELDWSPKFQELETIIESAWAWLKAHPFGYSQPSASSGA"
BASE COUNT         1223 a         1492 c         1343 g         1127 t
ORIGIN      
        1 cgttcccctt tatctggaat gcgttatctg cgggtcagga ctcccgctaa actactacct
       61 tcagtgcctc acgtttaccg cttgctatgc taccgctacg ggtttatctt tctttcgtcg
      121 gcctctccct tttccgattc aggtaaacga ctgagcatct atttctgatg ccgggcatcc
      181 aacgttgttg ccgcagcctc tagctgtgga aagaaggagc ttcgaccaat gaaaaagagt
      241 tttgcgattt tggtgctcct ctccctgtta acgctgtctg ctttttcggc gagcgcgcaa
      301 tcgcgcccgc gacgagtcgg acaaacaact ccaaccacag caccgaatag tccaaccaca
      361 aatcgcgcgc cagtgctcgg cggcaccaac actgccggaa atcaaagtgg cactgcgcag
      421 ccagctcctg aaaccggccc ggaagaagtg gatgccggcg atgtcattaa ggtcgacacc
      481 actctggtaa ctctgcctgt cagtgtcacg gatcgcgatg gtcgctacat tcctaactta
      541 acaaagtcag actttcgtct ctgggaagat ggcgtcgaac aacaggtagc gtttttcact
      601 tcagtcgata aacccttttc cgtggttttg gtgctggaca ctagtggctc tacacgattc
      661 aaaattgaag aaattcaaga cgcagcaatc gcgtttgtta accagttgcg gaccgatgac
      721 cgggtcatga ttatctcgtt cgacgacgat attagagtcc tcacagaatt cactggcgac
      781 cgtaaccgaa tgcgcgatgc catcatgcgg acgcgcaccg gaaatggcac acggctatac
      841 gacgcggtag acctcgtgat taatcaacga ctcaatcagg tcgatggccg taaggcggtg
      901 gtgctcttta ccgacggcgt cgacactgcc agccgccacg catcctatgc cagtaatgtc
      961 cgcgatgcag aggaacttga tgcgctaatt tatcccgtgc agtacgacac gtttcgtgat
     1021 gttggcggcg ggggcggcgg tgggatcggt tggccaggcg gccggggctc aaccaatcca
     1081 atcggcatcc taattgacat ttggggtcgc cgtggcggcc ggtcgggacc aattggtggg
     1141 ggtgggcgcg gtggtgttgg tggaaatcgc agcgactatg agcgcgccga tcgctatctg
     1201 cgagagttgg cacagaagac gggagcccgc cagtaccaag ctgacaacac gaataatctc
     1261 tcttatgcct tccgcaacat cgctgaagaa ctgcgacgtc aatacagcct tggctattac
     1321 ccgattcgcc cgcctcaacc cggtgaacgc cggcaggtgc gcgttcgggt caaccaacct
     1381 aatctcgcgg tgcgttcacg cgatagttat gtatttgggc aagacggcac ggtcaattct
     1441 caaaagaccg cgcagacacc agtgctgcgc cggaagatga ccaattaacc tgaagttgag
     1501 taagccggaa gagtaactgt gaggatggcg ccttggcctt cctctcggtt ttcagctttg
     1561 actgccccgc catggagctc aaccagatgc cgcgtaatgg ccaggcctaa tcccaacccc
     1621 ccgtgacgtc ttgtgctcga gctgtctgcc tgactgaatc tttcgaaaac gtgtggcaga
     1681 aagtcgggag gtatccccgg accgtcgtcc cagatcgcaa tctccgctga gccattcttc
     1741 cgctgcacga caatctccac gagtccacca ttcgcagtaa acttcacggc attagaaaga
     1801 agattccaaa tcacttgctg caaacgctcg gcatcaccgc tgactaacag ctccgggctg
     1861 ttgaaccgag tcttgagctt tatctctttt gcctcggcgg cgggacggac aacatccatc
     1921 gccgcctgga tcactgacgt caactcgagc ggcacaagtc caatgcgcag cttgcccgtg
     1981 atgatgcgag aaacgtcgag catgtcttcg atgatttgct tctgtgacca ggcgtttcgt
     2041 tcaatcactt caaaagcgtg ctcgaggttc ttgggatcaa gttgtcccga acgcagcaat
     2101 cgcgcccaac cgacaatcgc atttagtgga gtgcgcaact cgtgagatat cgtcgcgagg
     2161 aattcgtctt tcagacggct cgcctgctca gcctgagacc gagcttcccg ctcagactcc
     2221 aatagcctgg tccgctcttc ttgcgctcga acagtctgcg tgatgtccat ggtgacgccg
     2281 cgcatggcta ccggttttcc gctctcatcg caaaccacca ccgactgcga ctcaacccaa
     2341 atttcacgtc cgtcttttgt cagccaacga aaacgattca cgcctccagt gcccgaggcg
     2401 aaggtgcggg ctgcttcgcg cccggccgct tcgcgatcat cttcatgaac gatcgtgcgc
     2461 caaaaatttg gggtcgaaag ccattcttcc acactgtaac caagcatctt ctccacgtag
     2521 ttgctgacga aatcgatgcg ctggctatca gccgtcggct cgccccaggc ttcccatacc
     2581 acgcccggca catgcgcgac gatgtcttcg acacgcttct gctgtctggc aacttccgcc
     2641 accaggccta gtgtttcctt ttgtgcggtc ttgcgctcac acatcactgc gtaaagcacg
     2701 taagtaagca gagctgctga tccaacgaag atttgcagca gcaacaacga ttcgttgggc
     2761 ccgcccctga cgaaggaacc atagccatgg gccgtccccc aaactgcaac tgccgacatg
     2821 agcacggtcg cagacgtgac gattcgctga tccaaacgca gcgccgccca tacaagaaag
     2881 ggcattgtta agtgcgcaat cggataacgc ttgtccggac cggggatcca gccggcgaaa
     2941 gagataagcg aaacgagaaa cagagtcgcc agcagaatcg ctgcttctat cacagcttga
     3001 ggagatgtgc gccatttcag attcgtaccc caggccaaga ggaatggcgc cacaatcaga
     3061 gcgccaaagc cgtcacctag taaccaggta agccaaaggc tcccaaagtt cgcccactgc
     3121 gcttcgccgc cgatacacaa actgatattg ccaatcgttg agctaatcat cgaactgaaa
     3181 acaacagcga acagaacaaa cttaagcaca ctcctgatgg agctgaagga gtaatcaaaa
     3241 ggagatcgat agctgagcag gtatgaagcg gtgactgcct cgagggtgtt gccgatagca
     3301 atgcccagcg ccaccggcgt cgacacctca gtccaaagat tcgccaggaa ggccccaatc
     3361 agtaccgccg gccaaaggcg gcggccgaaa agcaagagcg cagcgatggc gataccagtc
     3421 ggcggccaaa ccggagaaac gttcgtatga acagaggcga gtgaaagtcc gagctcggcg
     3481 gctagaaagt atgtgagcgt gactgcaccc accaatccaa agtagcgcca ggatccgaat
     3541 ttcatcttag ataaaccagt tggactgttg atctgaaccg gagtcagatt ggagagatcc
     3601 gattgaagac tgaattgaaa gcctgctgga gcgaggcgag ccgtcaggcg acggcccgcc
     3661 gaactgtatg accacccctt gttcgttgta gataacatac tcacgtaatg ttcggatttt
     3721 gtgtcatgaa ggacacaatt tttgggatag acggtgaaca atacaaggtc gccaaaaacc
     3781 tcgatacttc aagcaccaga cgaggcgctt ggctggctgt aaccaaaagg gtgagcctta
     3841 agccaagccc acgctgattc aatgatcgtt tccaactcct gaaattttgg cgaccaatcg
     3901 agttcctgtt tgatggcaac ggagctcgca atcagggttg gagggtcacc agcccgacgg
     3961 ctcgtgacct taaccggaat cgcgcgacca gtgatctgtg ccgcgcaatc gatcacctct
     4021 ttgacagaat acccggcgcc gcagccaaga ttgtaaaggg cattccgcgg actttggagc
     4081 gctagaatat gtgcgcgcgc aagatccaca acatggatgt agtcccggac gcaggtacca
     4141 tccctggttg ggtaatcgtc accgaatagt tccacgtttg accgtgttcc ggcggccacc
     4201 tgtaagacaa tcggaatcaa atgagactcg ggtgaatgcc attcgccaca gcgcaacgtt
     4261 gcccccgcgg cattgaaata acgcagcgtt gtgaaccgca ggtcatatgc gtggctatac
     4321 catttaagcg cttgctcaaa ggctagtttc gtttcgccgt aggggtttgt aggttgggtg
     4381 ggatcacctt cctctatagg ctgctttggc ggctcgccat agaccgcggc cgtcgaagag
     4441 aacaccagtc ttgacactcc ttccgcccgc atcgcgtcaa gcagcgaaag tccggctaca
     4501 acattgttct cataatagct gcccggcttt tccactgatt caccgaccaa cgacgccgct
     4561 gccatgtgaa ccacagccgc gacatcgtgc tgtcgcagca cggctctcaa gcgttcgcta
     4621 tcgcgtaaat ctccctcggc gaattccgcc tccggcgcga ctgcttcacg atgccccctg
     4681 gacagattgt cataaacagt gacctcatgc ccatcacaca gaagttcttc aacgacaaca
     4741 ctgccgatgt atcctgcgcc cccagttaca agtacgttca tcagttttcc ttaacgactc
     4801 ggattagatc cctggtacgc actcctcgct gctcttcgga aagtagtgac cagagttgac
     4861 agctctaagg ttggcattat gtgatccgag acgctgctga tcaaagcgtg acgccgcgtc
     4921 cgtgtcgctc gggcactcaa tctcggacgg gctggcactc atcttgcgaa aaacaatctc
     4981 gcactggtta ttcgcctcgg aagtgttttg aaccgagtcg atctatatcc aagcgtattg
     5041 gttgagttca catccggcag ggggtgcagc gaatcgatta ccctggcttc actgttgaag
     5101 atacacttga agatcacctg agacgaattc tttgcagctt tttaagacaa caacctggct
     5161 tttcctttac caggacgaaa ccagt
//
LOCUS       DASUEX010000609         5763 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_791743, whole genome shotgun sequence.
ACCESSION   DASUEX010000609 DASUEX010000000
VERSION     DASUEX010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5763
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_791743"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            83..922
                     /locus_tag="VFV61_10115"
     CDS             83..922
                     /locus_tag="VFV61_10115"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX4900877.1"
                     /translation="MSETRNSYSSIDEAARDVERSSDPEQRVQDLVEQLKLRSAELEA
                     ANRELRRVSHYRSLFLARMSHELRTPLTSILGFSEILLDQEMLTETQRRFCKKIQDSG
                     MQLQTSLNQLVDLSRVEGGQTEVFLQEFSLQETLSESCQALARFAHVHQVAIDYEVGS
                     NVTTVVSDRGKLRQIVYNFLAWALSRSPADQRVSLNAKVTDGTLLHLQIDDAGPPIAD
                     LARVFDPEEKPGGREADINELGILIGHRLLEVMSGSVTLENRAEGGLRILMQLPARPA
                     KAY"
     gene            926..1705
                     /locus_tag="VFV61_10120"
     CDS             926..1705
                     /locus_tag="VFV61_10120"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX4900878.1"
                     /translation="MEEIKIRECLTIDELDHCVKLQRDVFGLPDLEISPRRHLVVSRQ
                     AGGWTLGAFTAEGRLAGFVHHLIAVRGVNEIVGYSHMMAIAREYQNQGIGARLKWAQR
                     ERALGEGRRFIKWTWDPLQSRNAHFNLNRLGVVVRGYAENFYGTDYGSDPDQAAAKGL
                     DSDRLFANWELDSRRVNQLASGGVLETEAEVKATVEIPADWSALVKTDVPRARAEQLR
                     VRAEFQAAFGAGLICAGFSRSAGRPRYLLYETKTIAASLEP"
     gene            1702..3132
                     /locus_tag="VFV61_10125"
     CDS             1702..3132
                     /locus_tag="VFV61_10125"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX4900879.1"
                     /translation="MSFTRFDRYVDRHARSFAERLQALCRMPSVAARGTGMRAMVEAV
                     EQAMQRVGIGTQLFKMGTGYPIIYGECGSGPAYLVYGHYDVQPVGHLTDWSVGPFSGS
                     ILDGKLYARGAANSKGDLLARLVAVEAYQKTFGKLPISMRFIVEGEDGLGSPNLFKFT
                     SEHTDLLAAKGCLWDEGYRDTKERPVISLGFKGITFLELRAHGARADLHSKWGAIVPN
                     PAWRLVQALATITSPKGVITIDGFSSHLAPINADDAEALKAIELDEVGLKKEFRIGGW
                     VRSMKGPALVKEHIFGPTCTICGIQTGHTDAGAKTVLPSSAMARLDFRLVPDLTPSLV
                     LELLRAHLDVRGFKDIEIIELGSAPLAKASATSRVARSVIEPAIEVYGTAALVYPMDP
                     SSGPVGAVCGVSSPATPVASFGVSYAGSNPHGPDENIRLDDFLLSVKFLGRVISRLGE
                     TRSEVKAEKLRTSGTRTTVIKEEVAQ"
     gene            3152..5182
                     /gene="ligA"
                     /locus_tag="VFV61_10130"
     CDS             3152..5182
                     /gene="ligA"
                     /locus_tag="VFV61_10130"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEX4900880.1"
                     /translation="MTTARSTRKAPTINKEILELRDEIRRHEELYYVNDNPEISDSEY
                     DALLARLQELETKHPDLITPDSPTQRVGGRPAEGFPEVVHRRPMLSLENTYNIEELVA
                     FDQRCQRLAEGRAVEYVAELKIDGVSISLQYEDQVLARGVTRGDGRIGEEVTQNARTI
                     RSVPLKLRAKSTPGAEIEVRGEVFIPRRVFERTNAEREEQGEPRFANPRNAAAGTLRQ
                     LDSKAVAKRGLDMFAYDLLAGERKPFPTHWEALDWMEAAGFRVNPHRERCQSIAEVIE
                     FCNRMESRRDELDYEIDGLVVKVNSTTLQDEFGSTAKAPRWAVAYKYPARQATTQVLS
                     IVVQVGRTGALTPVANLEPVLLAGTTVSRATLHNADEVKRLGVRIGDWVLIEKGGEVI
                     PKVLKVIESKRTGAEKPFRMPRKCPVCGGEISKVEGEVVSRCIAADCPAQLKGKLLHF
                     ASRRAMRIEGLGESLVDQLVQKGMLRDFGDLYSLTLDDVAGLERMAKKSAANLLAQIE
                     ASKTRDLPNLVYALGIRHVGDRTAGLLARHFGSLADLGKASVEELDDIPEIGLTVAES
                     VRDWFDDSGNLELCNRLDAAGVKTTLARSANQKADERFANKQFVLTGTLASFTREEAK
                     GLIESRGGRVMSSVSKKTDFVIAGEEAGSKLERATDLGVTVLDEPAFKKMLG"
     gene            5241..5678
                     /locus_tag="VFV61_10135"
     CDS             5241..5678
                     /locus_tag="VFV61_10135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900881.1"
                     /translation="MATVQTENENLTLLFTIFCDDVRLEAGNKLSFMGVFQNIMVQQL
                     PVTLIKFAVVNHWRGEGAHLSEVRILTPDRQHPVVVSQPTSFEIAPGGFADNISFFVN
                     VTFPAPGQYWVQTLVDSNLFDEQPLTVSDGREAQADSASEAVN"
BASE COUNT         1249 a         1489 c         1769 g         1256 t
ORIGIN      
        1 ttcggattaa gagcagagga agagtcggcc agagatcgtt gctatcggtg tctgccaggc
       61 cgcctgacac ccattgacca tcatgagtga gactcgcaac agttacagct cgatcgacga
      121 ggcagctcgt gatgttgaac gcagctccga tcccgagcag cgcgtgcagg atctcgtgga
      181 acagctaaag ctgcgttcgg cagagcttga agctgcgaat cgggagctgc gacgggtttc
      241 acactatcgt tccttattcc tggcgcgcat gtcgcacgag ttgcgcacgc cacttacttc
      301 aatcctgggc tttagcgaga tccttctcga tcaggaaatg ctgaccgaga cacaacggcg
      361 tttttgcaaa aagattcaag actccggcat gcaattgcag acgagtctga atcaactcgt
      421 agacctctcg cgcgttgagg gaggacaaac cgaagttttc ttgcaggagt tttccttaca
      481 agagacgctg agcgagtcgt gccaggcgtt ggcccgcttt gctcatgtgc accaggtggc
      541 gatcgattac gaagttgggt cgaatgtgac aacggtagtc tctgatcgcg gtaagttacg
      601 ccagattgtc tacaattttc tggcgtgggc tttgagtcgc agccctgccg atcagcgcgt
      661 ctcgcttaac gctaaggtca cagatgggac gctgctgcac ctgcaaattg atgacgctgg
      721 tccgcccatc gctgatctgg cgcgcgtgtt tgacccggaa gagaaaccgg gcggacgcga
      781 agcggatatc aatgaattag gaatactgat tgggcatcgt ttgttggaag tcatgagcgg
      841 cagcgtgact ctggagaatc gagccgaagg tggcctgcgc attctgatgc aattaccggc
      901 gcggccggca aaggcctact agcgcatgga agagattaag attcgcgaat gcttgacgat
      961 tgatgagctc gatcattgcg tcaagctgca acgagacgtt tttgggttac ccgatttgga
     1021 gatttctccg cgccggcatc tggtcgtttc gcgccaggct ggtggttgga cactcggagc
     1081 cttcaccgca gaaggccggc ttgcgggatt tgtgcaccat ctgatcgccg tccgcggagt
     1141 taacgagatt gtaggctact cgcacatgat ggcgattgct cgggagtatc aaaaccaggg
     1201 aatcggtgcg cgactgaagt gggcgcaacg ggaaagggcg ctcggcgagg ggcggcgctt
     1261 cattaagtgg acctgggacc ccttgcaatc gcgcaacgcc catttcaatt tgaatcgtct
     1321 cggcgtcgtg gtgcgcggtt atgccgagaa cttttatgga actgattacg ggagtgatcc
     1381 ggatcaggca gccgctaagg gtctcgacag cgatcggctt tttgcaaact gggaacttga
     1441 ttccagacgc gttaaccagc tggcgagtgg cggtgttctc gaaacggaag ctgaagtaaa
     1501 ggcaacggta gaaatcccgg ccgactggag cgctttagtg aagacggatg tgccgcgcgc
     1561 gcgtgcggaa caactgcgtg tgcgcgctga atttcaggca gcgtttggcg ccggtttaat
     1621 ttgcgcgggc ttttcgcgaa gtgccgggcg cccgcgttat ctcctttatg aaacgaaaac
     1681 gattgcggcc tcgctggaac catgagtttt actcgtttcg atcgctacgt tgatcgccac
     1741 gcgcggagtt tcgccgagcg tttgcaggct ctttgtcgca tgccttcggt cgccgcgcgc
     1801 ggtaccggca tgcgggcgat ggtggaagcg gtcgagcagg cgatgcaacg cgtcggaatc
     1861 ggaacgcagc ttttcaagat gggtactggt tatcccatca tctacggcga gtgcggcagc
     1921 ggcccggctt acctcgtcta tggacactac gacgtgcagc ctgtgggtca tctcacagat
     1981 tggagtgtgg gcccgttttc aggatcaatt ttggacggga agctttacgc gcgcggcgcc
     2041 gcgaatagca aaggagatct gctggcacga ttagtggccg tggaagctta tcaaaagaca
     2101 ttcggcaaac tgccgatctc tatgcggttc atcgtcgaag gggaagacgg gctcggcagt
     2161 cccaatcttt tcaagttcac cagtgaacac actgacttat tggcggccaa aggctgcctt
     2221 tgggacgaag gttatcgcga cactaaagaa cgcccggtaa tcagtctggg tttcaaaggc
     2281 attacgtttc tcgaattgcg cgcgcacggc gcccgcgccg atctgcactc aaagtggggc
     2341 gcaatcgtgc cgaatccggc gtggcgtttg gtgcaggccc tggcaacgat aacctcgcct
     2401 aaaggcgtga tcacaattga tgggttctct tcgcatcttg cgccgatcaa tgcggacgat
     2461 gctgaagcct taaaggcgat cgagctggat gaagtggggc tcaagaaaga gtttcgcatc
     2521 ggtggttggg tgcgttcgat gaagggaccc gctttagtta aggaacatat ctttgggccg
     2581 acgtgtacca tctgcggcat tcaaacagga cacacagatg cgggtgccaa gaccgttttg
     2641 cccagcagcg ccatggcgcg gctcgacttc cggctggttc ccgatctaac accgtcgctg
     2701 gtattggaac tgctgcgtgc acatctggac gtgcggggct ttaaggatat cgagatcatc
     2761 gagctgggca gcgcgccgtt ggcaaaagct tcggctactt cgcgcgtggc tcgttcggtg
     2821 atcgagccgg caattgaagt ttacggaacc gcggcgcttg tctatccgat ggatccttca
     2881 agtggtcctg taggcgctgt ttgtggtgtc tcgtctccgg caactccggt cgcctcgttt
     2941 ggcgtcagct acgcgggatc caatccgcat ggccctgacg aaaacatccg gctcgacgat
     3001 tttcttttga gcgtcaaatt tctggggcgc gtgatcagtc gcctcggaga gaccaggagc
     3061 gaggtcaagg ctgaaaaatt acgaaccagt ggaacgagaa cgaccgtgat caaagaagag
     3121 gtcgcgcagt gaattagagc aggagcgtgg attgactacc gccagatcaa ctcgcaaggc
     3181 gcccaccatc aataaagaga ttctcgagtt acgggacgag attcgtcgtc acgaggagct
     3241 gtactacgtc aacgacaatc cggaaatctc ggacagcgaa tacgatgccc tgcttgcccg
     3301 gctgcaggaa ctcgagacaa agcatcccga tctgattacc cctgatagcc ctacccagcg
     3361 cgtgggtgga cgtccagccg agggttttcc cgaggtcgtt catcgccgcc cgatgctctc
     3421 gctggagaat acttacaaca tcgaggagtt agtggccttc gatcagcgct gtcaacggct
     3481 cgctgaaggc cgagccgtgg aatacgtcgc ggaactcaag atcgacggcg tctcgatctc
     3541 gctgcagtat gaagatcagg tgctggcgcg tggcgttacc cgtggtgatg gccggattgg
     3601 cgaagaggtg actcagaacg cccggacgat tcgcagcgtg ccgcttaagt tacgcgcgaa
     3661 gtccactccg ggagcggaaa tagaagtgcg cggcgaagtc tttattcccc gacgcgtgtt
     3721 tgaacgcacg aacgccgagc gcgaagagca aggcgaaccg agattcgcga atccgcgaaa
     3781 cgccgcagcc ggaaccttgc ggcagctcga ttcgaaggcc gtcgctaagc gcgggctgga
     3841 tatgtttgct tacgacttgc tggccggtga gcgcaagccc tttccgactc attgggaagc
     3901 gcttgattgg atggaagccg cgggctttcg cgttaatcca catcgagagc ggtgccagtc
     3961 gatcgcggaa gtgattgagt tttgtaaccg catggaaagc cggcgcgatg agcttgatta
     4021 tgaaattgac gggctggttg tgaaggtaaa ctcgacaacg ctccaggatg aattcggatc
     4081 gacagcgaaa gctccgcgtt gggccgtcgc ctataaatat ccggcccggc aggcgaccac
     4141 ccaggttctc tcaatcgtag tccaggttgg tcgcacgggt gctctgacgc cggtagccaa
     4201 tctcgaaccc gtgttactcg ccggcaccac agtctcacgc gcgacgctgc acaatgccga
     4261 cgaagtgaaa cgtctgggcg tacgcatcgg cgattgggtg ttaatcgaaa agggcggcga
     4321 agttattccg aaagtgttga aggtaattga atcgaagcgg accggagcag agaagccgtt
     4381 tcgcatgccg cgcaagtgtc ccgtttgcgg cggcgaaatt tcgaaagtgg aaggggaagt
     4441 cgtttcgcgt tgcatcgctg ccgattgtcc ggcgcaactc aagggcaagc tgctgcactt
     4501 tgcttcgcgc cgcgccatgc gcatcgaagg tttaggcgag tcgctggtgg atcaattggt
     4561 tcagaaaggc atgctgcgcg attttggtga tctctatagc ctgaccctgg atgacgttgc
     4621 cggcctggaa cggatggcga aaaagtctgc ggcaaatctg ctggctcaga ttgaagcgag
     4681 caagactcgc gacctcccca acctggttta cgcccttggc attcgtcatg tcggcgatcg
     4741 caccgccggt ttgctggcgc gacatttcgg gtcgcttgca gacctgggca aagccagcgt
     4801 tgaagagtta gacgacattc ctgagattgg cctgactgtc gccgagagtg ttcgtgattg
     4861 gtttgacgat agtggcaacc tggagctctg caatcgcctg gacgccgcgg gcgtcaaaac
     4921 cacacttgcc cgcagcgcta atcaaaaggc ggatgaacgg ttcgcgaaca agcaattcgt
     4981 cttaacagga actttggctt cgttcactcg cgaggaagca aaggggctga tcgagtctcg
     5041 gggcggacga gtgatgtctt cggtgagcaa gaagacggat tttgtaatcg ctggcgaaga
     5101 ggcaggttcg aagctggaaa gagccacgga ccttggcgtg acggtactag acgagccggc
     5161 gttcaagaag atgttaggat aggggcacct attggcgatt gccaattgtc gaatgttgat
     5221 tagcgatacg agaggcttaa atggcaacag tacaaactga aaacgagaat ctgacattgc
     5281 tcttcacaat tttttgtgat gacgtccggt tggaagctgg caataagctt tccttcatgg
     5341 gtgtatttca aaacatcatg gtgcaacagc tacccgtcac tctgatcaag tttgccgtgg
     5401 tgaatcattg gcggggtgag ggcgcgcatc tttccgaagt gcggattctg acgccggatc
     5461 ggcagcatcc ggtagtcgtg tcgcagccga cgagttttga aatcgccccc ggcggctttg
     5521 ccgacaacat tagtttcttt gtaaacgtga ctttccccgc gcccgggcag tactgggtgc
     5581 agacgctggt agactccaac ctgtttgatg aacaaccgct gaccgtttcc gacgggcgcg
     5641 aagcgcaagc ggacagtgct tccgaagcgg tgaactgacc ccacgcgggc gcgcttccct
     5701 agttgcgcgc ccgcccacag acgaaacccg agttgttcaa cctaatactg gatgccgagt
     5761 gcc
//
LOCUS       DASUEX010000610         5381 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_791746, whole genome shotgun sequence.
ACCESSION   DASUEX010000610 DASUEX010000000
VERSION     DASUEX010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5381
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_791746"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..721
                     /locus_tag="VFV61_10140"
     CDS             <1..721
                     /locus_tag="VFV61_10140"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX4900882.1"
                     /translation="DEATDTVVFLGDLIDRGPNAPGCVDHVIKLCQANPERVLCLRGN
                     HEQMLLDFIEGTTNLWITPVTGGERTFEQYAGESLTLRSEQDLEDARHLIEEKIPGAH
                     LDFFRERPYYHEDEFAIYVHAGLDHGKHPRESSPQLLIWSRDMDFYKNYRGKPCVFGH
                     TPTPFLPLRGRVGRHGIYIFNSAIGIDTGYNHQSPLSCLSLPDFTLYQSFADGHEETH
                     QITSFIPETLKEMRKKAGLGR"
     gene            complement(1060..1728)
                     /locus_tag="VFV61_10145"
     CDS             complement(1060..1728)
                     /locus_tag="VFV61_10145"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEX4900883.1"
                     /translation="MLIDLSHTIEHGMITYKGLPAPIISDHLSRAASKSIYEAGTEFH
                     IGRIEMVANTGTYLDSPFHRYPEGKDLSQLELSSLANLESVLVRCLAAGSEPGQRGIS
                     PEALADRDLNGKAVLFHTGWDRHWRTDKYSDGTHPFLTAETAEILVKARPALVGIDSF
                     NIDDTSSGRRPAHSLLLAAEIPIVEHLCNLEALPETDFRFFAVPVKVKAFGTFPVRAF
                     ALRN"
     gene            complement(1818..2738)
                     /locus_tag="VFV61_10150"
     CDS             complement(1818..2738)
                     /locus_tag="VFV61_10150"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF045485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HEX4900884.1"
                     /translation="MTPFSLKDESELVPLADFFSRGRLLIDAKLDQLLPAESHEPVAV
                     HSAIRWSVFAGGKRFRPLLLLATGETLGAGPAELIATACALEMIHAYSLIHDDLPSMD
                     DDDLRRGRPTCHIRFGEATAILAGDALQSLAFQTIAEDETLDATRRLKLITELARAAG
                     TPQGMVAGQALDLEAESRQVSGAELEQSHGLKTGALIRAAARGGAFIANAGDKELNLV
                     TRYATQLGLLFQITDDLLDVTATAEALGKTPGKDARSSKATFPGIYGIEATRERARHV
                     HQEALTALAEMDCETVLLRAIADFILERKS"
     gene            complement(2735..3016)
                     /locus_tag="VFV61_10155"
     CDS             complement(2735..3016)
                     /locus_tag="VFV61_10155"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEX4900885.1"
                     /translation="MTNNETKTKSFESSLEALEQIVRQLEEGDLPLEKSLELFEQGIR
                     LSRECQDRLGQAERRIEILLRDNQGRPTVSEFAADQDRDGFPDLGEKLG"
     gene            complement(3006..4415)
                     /gene="xseA"
                     /locus_tag="VFV61_10160"
     CDS             complement(3006..4415)
                     /gene="xseA"
                     /locus_tag="VFV61_10160"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEX4900886.1"
                     /translation="MSQPLFSSLFDETERRPLTVSELTGSVRKALESRFSSVWVEGEI
                     SNFKAHSSGHWYFTLKDESAQLRAKCFRSTNTRIRFRPTDGLYVRARGRLSVYEPRGE
                     YELVVEALDPVGEGALRIAFEQLRDRLQTEGLFARELKRPLPVFPGRVGIVTSPTGAA
                     IRDILNVISRRTRTVHVLFSPARVQGEGAGRDIARAIRLINDHHQAMQRAGRSADLVD
                     VLIVGRGGGSSEDLWAFNEEEVARAIRASVIPVISAVGHETDFTIADFAADLRAPTPS
                     AAAELVAAKEDELCSTLAELGRNLSRLMRFRISAARNRVQHQALSQVFEEVRGRLRAA
                     HKETGDATHQLHLLLTHALRAAQGRRERIAFQLSPERLQARLEQARMRFDSAHIGCNA
                     AVLTRLETARTRLGLVATALDALSPLAVLQRGYAIAEQADGRLLRDASSVSPGDELRV
                     RLAKGRLTARVEDIQTNDE"
     gene            complement(4692..>5381)
                     /locus_tag="VFV61_10165"
     CDS             complement(4692..>5381)
                     /locus_tag="VFV61_10165"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX4900887.1"
                     /translation="SGSVEERIDFIRKAARDFLNTASPQDRISIISFRDDIQVISDFT
                     TNRRLLSKKLDEIDAGGATALYDALAYVVSEPLKKLRGERTAIVILSDGDDNKSFVPF
                     PALLDAFSESGALIYPLYVPSGLIPEASVPKSETTRDPLRTRYLTLTTRAEEEGRKLA
                     AVSGGVYYPVRRIEDLQRAYDDVVLQLRTAYTITYASNSTPSGNRRVRVRANREGASV
                     RLSPVVNVSNP"
BASE COUNT         1208 a         1493 c         1544 g         1136 t
ORIGIN      
        1 cgacgaagct accgatacgg tagtgtttct cggcgacttg atcgatcgcg gacctaatgc
       61 gcccggttgc gtcgaccacg taatcaagtt gtgccaggca aaccctgaac gcgtgctttg
      121 tctgcgcggt aatcatgaac agatgttgct ggacttcatt gagggcacca cgaatctgtg
      181 gatcacaccg gttaccggag gcgagcgcac gtttgaacaa tacgcgggtg aatcgctgac
      241 gctaagaagc gaacaggacc tggaagatgc gcgccactta atcgaggaaa agattccggg
      301 cgcacatctt gatttctttc gcgagcgccc ttactaccat gaggacgagt ttgcgatata
      361 cgtccatgca ggtctggatc acggcaagca tccgcgtgag agctcacccc agttgttgat
      421 ttggtcccgc gacatggact tctacaaaaa ctaccgcggc aagccgtgcg tttttggaca
      481 tacgcccacc ccttttcttc ctttgcgcgg gcgagttggg cgtcatggga tttatatctt
      541 taacagcgcg attggcatcg acaccggcta taaccatcag tccccgctca gctgtctttc
      601 ccttcctgac tttacgcttt accagtcctt cgccgacggc cacgaggaaa cacaccagat
      661 cacttctttc attcccgaga cgttgaaaga gatgcggaag aaggcgggtt tgggacggta
      721 gcaagtaggt cggggaattt atcttctaaa aaacatttcc catttctcat ttttgattgc
      781 gcctacggca agcgcagagt cgcaatcgtg cagaggcgca gagaaaacat tcaaccgttt
      841 caacgagatt aagttttagt tgggtggatc ttttctgctg agcggcagaa gtacccacga
      901 aatcacacta acaacgagat acattcttgg ctttctctgc gctctcggcg gcttagcgcc
      961 ttggcggttt cgttttccaa tgagaaatgg aaaatcatgg aaaatgaaaa atgagaaagg
     1021 gaaagtattt ggcgaggaca ccttgaaatc cagaattgac taattacgaa gtgcgaaagc
     1081 ccgcacgggg aaggtgccaa aggccttcac cttcaccggt acggcgaaga agcggaagtc
     1141 cgtttctggc agcgcctcga ggttgcaaag gtgctcaacg atgggaatct cggcggcgag
     1201 gagcagggaa tgggcgggac gccggccgct actggtatcg tcgatattga aagaatcgat
     1261 gcccaccagg gcaggtcgcg cttttacgag gatctctgct gtttcggcag taagaaaggg
     1321 atgggtgccg tctgagtatt tgtcagtgcg ccagtgtcga tcccatccgg tatgaaacaa
     1381 gacggcctta ccgttcaagt cgcggtcagc cagagcttcc ggagagatgc cgcgctggcc
     1441 aggctcactg ccggcagcta aacaacgcac cagaacgctc tcaaggttgg caagcgagct
     1501 aagctcaagc tgcgagagat ccttgccttc cgggtaacga tgaaaagggc tatcgagata
     1561 ggtgccggtg ttggccacca tttcgatcct gccgatgtga aactcagtgc ctgcctcata
     1621 aatcgatttc gaagcggcgc gactcaagtg atcggagatg atcggagcgg gcagaccctt
     1681 ataggtgatc atgccgtgct cgatggtgtg acttaagtca atcagcataa gacttggcag
     1741 tagcaagtag tcagttgtcg gttgtcagta agtccactac cactgactac agtctactgg
     1801 ctactgacca ctgacactta agactttctt tccaaaataa aatccgcgat ggccctgagc
     1861 agcactgttt cgcaatccat ctccgcaagc gccgtgagag cctcctgatg cacatgccga
     1921 gctctttcgc gggtcgcctc aattccatag atgccaggaa aagttgcctt acttgaacga
     1981 gcatccttgc cgggagtctt accaagagcc tcagccgtcg cagttacatc aagcagatca
     2041 tcagtaatct gaaacagcaa gccaagctgc gtggcgtacc gcgtcacgag attgagttcc
     2101 ttatcgcctg cattagcaat gaaggcgcca ccgcgcgccg cagccctaat caaggcgcca
     2161 gtctttaagc catgactctg ctccaactct gctccgctga cttgccgcga ctcagcttcg
     2221 agatcgagag cttgtccggc caccatgccc tgcggcgttc ccgccgcgcg agctagttca
     2281 gtgatcaact ttagccgtcg cgttgcgtcg agagtctcgt cctcggcgat ggtttggaag
     2341 gcgagacttt gcaaggcatc gccggccaga attgctgtcg cttctccaaa gcggatgtga
     2401 caggtggggc ggccgcgacg caagtcgtca tcgtccatcg aaggcagatc gtcatgaatc
     2461 agggagtagg catgaatcat ctcgagcgcg caggcggttg ctatgagctc ggcgggtcct
     2521 gcgccgagcg tctcgcccgt agcgagcagc aacaacggtc gaaagcgctt accgcccgca
     2581 aaaacgctcc agcggatcgc gctgtgaaca gccaccggct cgtgcgactc cgcgggaagc
     2641 agttgatcca acttcgcgtc gatcagcaag cggccacgac tgaagaagtc agccagcgga
     2701 acgagctcgg attcatcctt gagtgagaac ggcgtcatcc caatttttcc ccgagatcag
     2761 gaaagccatc gcgatcctga tcggcagcaa actcactgac cgtgggtcgg ccctggttgt
     2821 cgcgcagtag gatctcaatg cggcgttcgg cctgacccaa ccgatcctga cattcacgcg
     2881 agagccggat gccctgctcg aagagctcca gactcttttc gagcggcaga tcgccctcct
     2941 caagttggcg gacgatttgc tcgagtgcct caagtgatga ttcgaagctc ttggttttag
     3001 tttcgttatt cgtcatttgt ttggatgtct tccactcgcg ctgttagccg tccctttgcc
     3061 aggcgaacgc gcaattcatc gccgggcgaa actgaactcg cgtctcgtaa caagcgcccg
     3121 tcggcttgtt cggcaatcgc ataaccgcgt tgcagtacgg cgagcggcga aagcgcgtcg
     3181 agcgcagtcg ccacgagccc caaacgcgtg cgggccgttt ccaggcgcgt cagaacagca
     3241 gcattgcagc caatatgagc cgagtcgaat cgcattctgg cttgttctaa acgcgcctgc
     3301 aaccgctcgg gagatagttg gaaagcgatt cgctcacgac ggccctgcgc ggctcgcagg
     3361 gcatgcgtca gcaacaggtg cagctgatgg gtagcgtcgc cggtttcctt atgagctgct
     3421 cgcagtcgtc cgcgtacctc ctcaaaaacc tgtgacagtg cctgatgctg gacgcgattg
     3481 cgtgcggcgc tgatgcggaa gcgcataagc cgggaaagat tcctgcccaa ctccgcaaga
     3541 gtggagcaga gctcatcttc cttcgccgcc acgagctccg ctgcggctga gggagttggg
     3601 gcgcgtaagt ccgcggcaaa gtctgcaatt gtaaaatctg tttcgtgacc gacggctgag
     3661 atgacgggga tcaccgaagc gcgaatggcc cgcgcaactt cctcttcgtt gaaggcccaa
     3721 aggtcttcgc tggaaccgcc accgcgtccg acgatgagca catcgacgag gtcggcagat
     3781 ctaccagcgc gctgcattgc ctggtgatga tcgtttatga gccgaattgc ccgtgcgatg
     3841 tcgcgtccgg cgccttcgcc ctgaacgcgg gctggggaaa agaggacgtg caccgtgcga
     3901 gtgcgccgtg agatcacgtt gaggatgtca cgaatcgctg ctcccgttgg cgacgtcacg
     3961 atcccgactc gaccggggaa aactggaagt ggtcgcttca gttcacgcgc gaataagcct
     4021 tccgtctgca gtcgatcgcg caattgctcg aaggcaatgc gcaaggcgcc ctcgcctacc
     4081 ggatcgagtg cctccaccac cagttcatat tcaccgcgcg gctcgtagac actgaggcgt
     4141 ccccgggcgc gcacatacaa accgtcagtg ggacgaaaac gaatgcgcgt attagttgag
     4201 cgaaagcact tggcgcgcag ttgcgcgctc tcatctttca gcgtgaagta ccagtgaccg
     4261 gatgagtgcg ccttgaaatt tgaaatttca ccttcgaccc agacagacga gaaacgcgac
     4321 tccagtgctt tgcgcaccga cccggtcaac tctgagacag tcagagggcg acgttcggtt
     4381 tcatcgaaca gggaagaaaa gagcggctgg ctcataggga tgaaaaggat gcaggaaggg
     4441 ggatgaaaag taaactggga gcggattgag cttcgatcct ggatcttagc aaggtccaat
     4501 taaccgccaa tgccaagaaa aagtctctga ctgccggtgc gcggcaagtc tttgacttgc
     4561 cgccattcgt ctcattaatc aggtcttcga cctctgcatt aggtcacaga cccaattccg
     4621 ttccggcgtg acggcaagtc aaagacttgc cgcgcaccag ctagtcagag acttgccgcg
     4681 caccgaagcg gttaaggatt ggagacgttc accaccggac tgagtcgcac cgatgctcct
     4741 tcgcgatttg cccggacgcg gactcgccgg ttgcctgatg gtgtcgagtt cgaagcgtag
     4801 gtgattgtgt aggcggttcg cagttgcagc accacgtcgt cataggcgcg ttgtaagtct
     4861 tcgatgcgcc gcaccgggta atagacgcca cccgacactg ccgcgagttt gcggccttct
     4921 tcttcagcgc gagtggtcag cgtcaaatag cgagtgcgca aagggtcgcg ggtagtttca
     4981 gatttcggca cgctcgcttc cggaatcagt ccagaaggaa cgtacagcgg ataaatcaac
     5041 gcgccagatt cgctgaaggc gtccagcaag gccggaaacg gcacgaacga cttattgtcg
     5101 tcgccgtcgg agaggatgac gatcgcggta cgctcgccgc gcaatttctt caacggctca
     5161 gaaaccacgt aggcgagcgc gtcgtagagc gcggtggcgc cgccagcgtc aatttcgtcg
     5221 agttttttcg acaacaaacg ccggttggtg gtgaagtcag aaatcacctg aatgtcgtcg
     5281 cgaaagctga tgatcgaaat gcgatcctgc gggctagccg tgttgagaaa atcgcgggca
     5341 gctttgcgaa tgaaatcgat ccgctcttcg acgctgccgg a
//
LOCUS       DASUEX010000611         2225 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_320995, whole genome shotgun sequence.
ACCESSION   DASUEX010000611 DASUEX010000000
VERSION     DASUEX010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2225
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320995"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="VFV61_10170"
     CDS             complement(<1..332)
                     /locus_tag="VFV61_10170"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX4900888.1"
                     /translation="MTLSSGTKLGHYEIRSLVGAGGMGEVYLAVDTELDRTVAIKILP
                     ESLALDPQRLQRFIQEAKAASALNHPHILTIHEIGTTGASRFIATEFIDGDTLRHRIG
                     SGMKLEEIL"
     gene            complement(506..1249)
                     /locus_tag="VFV61_10175"
     CDS             complement(506..1249)
                     /locus_tag="VFV61_10175"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX4900889.1"
                     /translation="MGTAATNTAVAIVIVGMLLIGCSSKPQTKADNNITPPSASMSPA
                     LIEGPVNYLALGDSTGIGVGARNGGYVARIFNRILSQRPGSKLTNLCVSGATSSDVLE
                     LQLDRGLTTDPNLVTLGIGINDIGHGFSAEEFARNYEQIVSTLRNKTRARIIVTNIPD
                     ISSAPRIPQFARAQYQQEIVNFNQRLEAIAARYEVTVFDVYTTTREQLPAHPEFFSGD
                     GFHPSDAGYELWANEMWPVVSGSIGLDSQ"
     gene            complement(1287..1859)
                     /gene="efp"
                     /locus_tag="VFV61_10180"
     CDS             complement(1287..1859)
                     /gene="efp"
                     /locus_tag="VFV61_10180"
                     /inference="COORDINATES: protein motif:HMM:NF001810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEX4900890.1"
                     /translation="MALIGANELKRKLLITVEGQPYVVLEVFFASPTARGAATMVRTR
                     LRQLLTGAVLEKSFRSSEKFEEPDVELAPASFLYSDGDGCHFMEEKSYEQFAFSSDEI
                     GNDRNYLKEGISLQVLKYNGAPVSLQLPQYVELAVVSTELGLRGDTAAGGATKMATLE
                     TGLEARVPLFIKEGEVVRVNTQTGEVTGRA"
     gene            complement(2018..>2225)
                     /locus_tag="VFV61_10185"
     CDS             complement(2018..>2225)
                     /locus_tag="VFV61_10185"
                     /inference="COORDINATES: protein motif:HMM:NF019758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900891.1"
                     /translation="AGAAARWAAGMEWSNLVQKTRAEEGDLFRMLSRTGEALLQIAGL
                     RKAHLQAAHVARIAAESVLREPVR"
BASE COUNT          499 a          616 c          581 g          529 t
ORIGIN      
        1 aggatctcct ccagcttcat tccggatccg atgcggtgtc gcaaggtgtc tccgtcaata
       61 aactcagtag cgatgaaacg ggaagccccc gttgtaccga tctcatgaat ggtgaggatg
      121 tgcggatggt tcaaagctga cgcggccttt gcttcctgga tgaaacgctg caggcgctga
      181 gggtccaggg ccaacgattc aggaaggatc ttgatcgcca ccgtgcgatc cagttctgtg
      241 tcaacggcca gataaacttc acccatgcca ccggcgccca ccagggaacg aatttcgtag
      301 tggcctagtt tggttccgga ggaaagtgtc atttatctta cgagacgatt catccttgtc
      361 cgctcagtga tccgggcatc ttgagttaaa tgcgcgcccg gatgttacga gtcttgctgg
      421 tggcgagtaa agagcattcg gtaatgcggc taattcgaga ctggccccgt ttcggttttt
      481 ccagccccaa tacgcccagg aagagttact gcgaatcgag accaatggag ccagaaacta
      541 ccggccacat ctcgttcgcc cagagctcat aacccgcgtc ggaaggatga aatccatcac
      601 cggagaagaa ctccggatga gcaggaagct gttcgcgcgt agtggtgtac acatcaaaca
      661 ctgtcacctc gtaacgcgca gcaatggctt ccaaacgctg gttgaagttg actatctcct
      721 gttgatattg agcgcgggca aactgaggga ttcgcggcgc gctggagatg tcggggatat
      781 tggtgacgat gattcgcgcc cgagttttgt tccgcaaagt gctgacgatc tgttcatagt
      841 tgcgtgcaaa ctcttcagcg ctaaagccgt gaccgatatc gttgatgcca atgccgagcg
      901 tcacaagatt tggatcagtt gtaagaccgc gatccagctg gagctccaac acatcggacg
      961 aggtggctcc actgacacac agattagtta gctttgagcc gggacgttgg gaaaggatgc
     1021 gattgaaaat ccgagcaacg taacccccat tgcgagcccc gacgccgatg ccggtcgaat
     1081 ctcctaaagc gagataattt accggacctt cgattagcgc tggggacatt gatgcggatg
     1141 gcggggtgat gttgttgtct gccttagttt gcggtttcga gctacaacca atcagcagca
     1201 ttcccacaat cacgatagcg acggccgtgt tggtcgctgc tgttcccact tcgattcctc
     1261 cggctgcgct ggagctatcg cgtgacctac gctctgccgg tgacttctcc agtttgcgtg
     1321 ttaacgcgca ctacctctcc ttctttgatg aaaagcggca cgcgggcctc caagccagtt
     1381 tccaacgttg ccattttggt ggcgccaccg gctgcagtat ctccgcgcag acccaactct
     1441 gtcgaaacga ctgcaagctc gacgtattgt ggcagttgca acgaaaccgg ggcgccgttg
     1501 tatttcagaa cctgaaggga aataccttct ttcaggtaat ttcgatcatt gccaatctca
     1561 tcgctggaaa aagcgaactg ttcgtaactc ttttcctcca tgaaatgaca gccgtcgcca
     1621 tcgctgtaaa gaaatgaagc cggagcgagt tccacatctg gctcctcaaa cttctcgctc
     1681 gaacgaaagc tcttctccag cacggcgccg gttagtaatt gccgcaatcg cgtgcgcacc
     1741 atggtcgccg cgccgcgcgc agtgggtgat gcaaaaaaga cttcgaggac aacatacggt
     1801 tggccctcaa cagtaatcag caacttgcgt ttaagttcat tggcgccaat cagggccatg
     1861 cttgaatcct ttctccagct aaccgtaagc ggtttttgtt gagttaaggt tttcccgctc
     1921 taaactttct tagtgaggtt tcgtgtcttt ttgtaggttg taacgctaat ctttggcaat
     1981 acgatccatc aacacgaaac gcacgaaaca acaaacacta cctcaccggc tctctcaata
     2041 ccgattcggc agctatccgc gccacgtgcg ctgcctgtaa gtgcgccttt cttaggccgg
     2101 caatctgcaa cagagcttcg ccggttcgcg agagcatgcg aaacaaatcg ccttcctcgg
     2161 cgcgtgtctt ctgcaccagg tttgaccatt ccattcctgc tgcccagcgc gccgccgctc
     2221 ccgct
//
LOCUS       DASUEX010000612         2627 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_535072, whole genome shotgun sequence.
ACCESSION   DASUEX010000612 DASUEX010000000
VERSION     DASUEX010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_535072"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            248..1351
                     /locus_tag="VFV61_10190"
     CDS             248..1351
                     /locus_tag="VFV61_10190"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF006707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HEX4900892.1"
                     /translation="MATQLSRTGTSLGRIEELLGANARTLLDHKSQTIPREQINLPGP
                     DFIDRVWAQSDRSPAVLRSLQSLFNNGRLAGTGYLSILPVDQGIEHSAGASFAPNPQY
                     FDPDNIVKLAIEGGCNAVASTFGVLGTVARRYAHKIPFIVKINHNELLTLPNKFDQVL
                     FGTVKEAHNMGAVAVGATIYFGSDESTRQITEIGEAFAQAHELGMACILWCYLRNSKF
                     KTADGDMHASADLTSQANHLGVTIQADIIKQKLPERSGGYGSVEKALGSSYGKTSKIV
                     SEKLLSDNPIDMVRYQVANCYMGRCGLINSGGESKGASDLAEAVRTAVINKRGGGTGL
                     ISGRKAFQRPMSEGVELLNAIQDVYLSEEITVA"
     gene            1552..1770
                     /locus_tag="VFV61_10195"
     CDS             1552..1770
                     /locus_tag="VFV61_10195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900893.1"
                     /translation="MAQDKKKEPPPVDNVISDQTGQFDLRFVLWRHFCSTNNIPVESL
                     PSQLNDEQKEKWEALKASRLRSPGQKEQ"
     gene            complement(2005..>2627)
                     /locus_tag="VFV61_10200"
     CDS             complement(2005..>2627)
                     /locus_tag="VFV61_10200"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX4900894.1"
                     /translation="KRAICIDPGDDSDEIVRAIDKHGFALEVIACTHAHLDHVGGVAA
                     LKQLKPAARIMLHQADEFIYQALPGQAGWIGIPRSQWAALGFDFAQPPAVDEYFNDGD
                     ILRVGEMEFQVLHCPGHTPGHVVLFAAAEQKVFVGDVLFAGSVGRTDLPGGSSSQLIA
                     SINEKLLPLGDDVEVYSGHGPVTTIGIERTTNPFLTGVYALGSGRM"
BASE COUNT          653 a          726 c          674 g          574 t
ORIGIN      
        1 ttcagttttg gagtttcagg gaacatcgtg aagtcgtgag ctgcgccccg ggaaagttga
       61 attgccgctt cggcagcaat cgaggagcat agtttacccc agtatctacg tgccgaaaat
      121 caaataatag actggccccc cggcgccctg gcgcctccga ttagagcggc catgcgtgtg
      181 ttataatttc gcctcgcgtt tccacaaaat attcccgacc cgccatcaat tcaggaggac
      241 ttaaacaatg gctacccaac tcagtcgaac cggtacgtca ctcggcagga ttgaagaatt
      301 attaggagca aacgcgcgca ccttgcttga ccataagtcg cagacgatcc ccagggaaca
      361 gatcaatttg ccgggcccgg atttcatcga tcgcgtttgg gcacagtccg atcgctcacc
      421 ggcggtctta cgttcgctgc agtcgctctt caataatggc cggctagcag gcacaggcta
      481 tctttcgatc ttgccggtcg atcagggtat cgaacactca gccggagctt cctttgcgcc
      541 gaacccgcag tactttgacc ctgacaacat cgtcaaactt gccatcgagg gcggctgtaa
      601 tgccgtggcg tcaactttcg gcgtgctcgg taccgttgcg cgacgttacg ctcacaaaat
      661 ccctttcatc gtaaaaatca atcacaacga gttgctgacc ctgcctaata agtttgacca
      721 ggtgcttttt ggcaccgtga aagaggccca caacatgggt gcggtcgcgg tcggcgccac
      781 gatctatttc ggatccgacg aatcgacgcg ccagatcacg gaaattggcg aagcctttgc
      841 ccaggcacat gagctgggca tggcatgcat tctttggtgt tacctgcgaa attcaaaatt
      901 caaaactgct gacggcgaca tgcatgcttc agcggacctg accagccagg caaatcacct
      961 cggagttacg attcaggcag acatcatcaa gcagaagctg cccgaacgca gtgggggtta
     1021 tggatccgtc gagaaggccc tggggagttc ttacggaaag accagcaaga tcgtgagcga
     1081 gaaactgctc agcgataacc caatcgatat ggttcgctat caggtggcca actgctacat
     1141 gggacgttgc ggtctaatca actctggtgg tgagtcgaaa ggcgcgagtg acctggccga
     1201 agcagtgcgc actgcggtca tcaacaagcg tggcggcggc accgggctca tttctggacg
     1261 taaggcgttt cagcgtccga tgagcgaagg cgtggagttg ctcaacgcaa ttcaggacgt
     1321 ttatttgagc gaagaaatca ccgtggctta agggagacct tcggcgacga cgtgtcgcgt
     1381 ttcaataaat cagccacaga tttccgcgga tgacacagat cagaactttt cccgcgtcat
     1441 cggcgtgaat tctgtggttg attatttcta cagcgggctc ttcgtgcgcg actaagggga
     1501 aaacctgcta cactttcggc aaatcaataa cagaccaaac gagtgggaac catggcgcag
     1561 gataaaaaga aagaaccgcc cccggtagat aacgtgatct cagaccagac gggtcagttt
     1621 gatcttcgct ttgtgctgtg gcgtcacttc tgctcgacaa acaatatccc cgttgaatct
     1681 ctgccgagcc aactcaatga cgagcaaaaa gaaaagtggg aggcacttaa ggccagtcgg
     1741 ctccgctcac ctgggcagaa ggaacagtga cagtcggtcg agaacaaaaa cattttccat
     1801 atatcattcc ccgtttttca ttttttattg gaactgaaac cgccaagccg ccaagaagcg
     1861 cagagcacgc caagaataaa acaaactgag tactttgttt ctttgcgctt tcttaattgc
     1921 tcagccttga ttgcacctcc caagttcaat cagcgaacaa agctcaaaga accaagttca
     1981 aagctcgttt ttattccttg gcgttcacat ccgcccgctt cccagcgcat agactcccgt
     2041 cagaaacgga ttggtcgtgc gctcgatgcc aatcgtcgtg accggcccat ggccggaata
     2101 aacctcgaca tcatcaccca gcggcaacag tttttcattg atcgaagcga tcagctgaga
     2161 tgaagatccg cccggcaaat ccgtgcgtcc caccgaccca gcgaagagta cgtcgccgac
     2221 gaagaccttc tgctcagccg ccgcgaacaa tacgacatga cccggcgtgt ggccgggaca
     2281 atgcaacact tgaaactcca tttctccgac acgcaaaatg tcgccgtcgt taaagtactc
     2341 atcgacggca ggtggctgcg caaaatcaaa tcccaatgca gcccactgtg agcggggtat
     2401 gccaatccat ccggcctgcc ccggaagcgc ctgataaata aactcgtccg cctggtgcag
     2461 catgattcgc gctgctggtt ttagttgctt gagcgcagcg actcctccca catgatcgag
     2521 atgtgcatga gtgcaagcga tcacctcaag tgcaaatccg tgcttgtcga tggcacgaac
     2581 gatttcgtcc gaatcatctc cgggatcaat gcagatcgcg cgtttgg
//
LOCUS       DASUEX010000613         2547 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_321262, whole genome shotgun sequence.
ACCESSION   DASUEX010000613 DASUEX010000000
VERSION     DASUEX010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_321262"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1200
                     /locus_tag="VFV61_10205"
     CDS             <1..1200
                     /locus_tag="VFV61_10205"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4900895.1"
                     /translation="QVVSQLGDWFNTIALYTIILNLTGSGRDIGLLLVARFVPSFIFG
                     PSSGVLADRFSRRSIMIYSDLLRALVVLGFLFVRRADQLWLIYVLTVLQLGLSTFFEP
                     AKTAAIPSLVADRELVAANAISSVTWSVMLTMGAAIGGLLTGWLGTDVAFVLDSGTYL
                     LSAALIASVKVPKRPKSKRGKFAIGRALGISETVEGIGYVKRRPRVLALLLVKPAWGL
                     GGGILTLLAVFGERVFPLGGSPAVGIGALFAARGIGTAIGPIVARRIAGEGKSQQQAS
                     IGIAFVIGGLFYVAFGYTASFVVALLVLGIAHTGGSILWVFSTVLLQREVEDRFRGRV
                     FAAELALLTLTLAASNYLTGELLDRFGYSARGLAMAIGVFFMIPGIVWFLTKRWWDRD
                     SPVGKPS"
     gene            1197..1739
                     /locus_tag="VFV61_10210"
     CDS             1197..1739
                     /locus_tag="VFV61_10210"
                     /inference="COORDINATES: protein motif:HMM:NF037785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900896.1"
                     /translation="MRIKVGVMGSAAEIEPGQRAEFERKLTQLCTALAAHDVVLFTGA
                     TSGLLREVGKAARTAGVFHVGISPASSLEEHISRFGLPSDACDVLVYTGFGLKGRNVV
                     LVRSCEIVMFVAGAMGSLNEFTIAHDEGKVIGCLRGTGGVADQADYLLQTFSKPSTAR
                     VFQDDDPEALLNACLSALGR"
     gene            1897..2541
                     /locus_tag="VFV61_10215"
     CDS             1897..2541
                     /locus_tag="VFV61_10215"
                     /inference="COORDINATES: protein motif:HMM:NF018190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HEX4900897.1"
                     /translation="MQSNDLVRAQHIPGEIAVDDFDHSAWTLSQPAFIAQYWSGVPAP
                     VHRHAEARLLWSNSALYARFVCPQEEPLVISSTPQTDAKTLGLWDRDVCEVFLAPNPS
                     APNHYFEFEAAPTGEWVDLEIQKNGDQRETNTVFHSGMAASASIRSQLVLVALRIPWS
                     ESLPAPKSRERWRINLFRCVGKDPDRGYVTWRPTFTTEPSFHAPEAFGWLVFGE"
BASE COUNT          466 a          698 c          768 g          615 t
ORIGIN      
        1 caggtcgtca gccagttggg cgactggttc aatacgatcg cgctgtacac gatcattctg
       61 aatctgaccg gctcagggcg agacatcggg ctgctgctgg tggcacgctt tgtcccaagt
      121 ttcatttttg gaccgtcgtc cggggtgctc gctgatcgct tcagccggcg ctcgatcatg
      181 atctattcgg atctgttgcg agccctcgtc gtcctcggct tcctgtttgt gcgtcgcgcc
      241 gaccagctct ggctaattta tgttctgacc gtcttgcaac taggtctttc cacgtttttt
      301 gagcccgcga agacggcggc tattccttcg ctggttgccg atcgcgaact ggtggcggcc
      361 aatgccatct cttctgtgac gtggtcggtg atgttgacga tgggtgccgc cattggtggg
      421 ctactgacgg gctggctggg aaccgatgtg gcgttcgttc tcgactctgg aacctatcta
      481 ctctcggcgg ccctgattgc cagcgttaag gttcccaagc gccccaaaag taagcgcggc
      541 aaatttgcaa ttggcagagc tctgggaatt tcagagactg ttgaggggat cggttacgtg
      601 aaacgacgcc cacgcgtgct ggcactgctg ctcgtcaaac cagcttgggg tctgggcggc
      661 ggcatactca ccctcctggc agtctttggc gaacgggtct tccctctcgg cggtagtccc
      721 gcggtaggga tcggagcgct ctttgcggcg cggggtattg gcaccgccat cggaccgatt
      781 gtggcgcggc gtatcgctgg cgagggcaag tctcagcagc aggcctcaat cggcattgcg
      841 ttcgtcatag gcggactgtt ctacgtcgcg ttcgggtaca ccgcgagctt tgtggtcgcc
      901 ttgcttgtat tgggaatagc gcatacgggc ggcagtattc tgtgggtatt ctccacagtg
      961 ctgctgcaac gcgaagtgga ggatcgtttt cggggtcgcg ttttcgctgc cgagttggcg
     1021 ctgctaacgc taacactcgc agcctccaac tatctgacgg gggagttgtt ggatcgattt
     1081 ggctattcgg ctcgcggcct tgccatggca atcggagtct tctttatgat tcccgggatc
     1141 gtctggtttc tgaccaaacg ctggtgggac cgcgatagtc ccgtggggaa accatcatga
     1201 gaatcaaagt cggcgtgatg ggttcggcgg ccgagattga gcccggtcaa cgcgctgagt
     1261 tcgaacgcaa gttgacgcaa ctctgcactg cactcgcggc tcatgacgtg gtgctcttca
     1321 ctggtgctac gagcgggttg ctgcgcgaag tcggcaaggc agcgcggaca gctggtgtct
     1381 tccacgttgg catctcgcca gcaagttcgc tggaagaaca catctcacgt tttgggcttc
     1441 cgagtgatgc ctgcgatgtc ttggtttata ccgggttcgg tctgaagggt aggaatgtag
     1501 tgctcgtgcg ttcgtgtgag attgtcatgt ttgtcgccgg agcgatgggc tcgctcaacg
     1561 aattcacgat cgcgcacgat gagggcaagg tgattggctg tttgcgcgga accggcggcg
     1621 ttgccgacca ggcagattat ctgttgcaaa cgttctcgaa gccgtccaca gcgagagtct
     1681 tccaggacga cgatcccgag gcgttactta atgcctgctt aagcgctttg gggcgctgac
     1741 cctgtttccg tttgcctaac ctgactatca aattgaaagt ctaccgcctt cgggccagcc
     1801 gcgtgaacgc ggcagttaag ctgcgtcgtc atcaggaggt cgagagacgc aaagccaatc
     1861 gagccgacct gcccggtgga actgacgtca acacaaatgc agtcaaacga tctagtaaga
     1921 gcccaacata tacccggtga aattgcggtc gatgacttcg atcattcggc ctggacgttg
     1981 agccagccgg cattcatcgc tcaatactgg tccggcgtgc ccgctccggt tcaccgccac
     2041 gccgaagctc gactgctttg gtctaactcc gccctttatg ctcgcttcgt ctgccctcaa
     2101 gaggaaccgc tcgtcattag ttcaactcca caaacggacg cgaagaccct ggggctctgg
     2161 gatcgagatg tctgcgaagt cttcctggca ccgaatcctt ctgctcccaa tcactacttc
     2221 gaatttgaag ccgcaccgac tggcgagtgg gtagacctgg agattcagaa gaacggcgat
     2281 cagagggaaa cgaacactgt gttccattca ggaatggcag cgtccgccag cattagatct
     2341 caacttgttt tagtagcgct gcggatcccc tggagtgaaa gtcttcctgc gccaaaatca
     2401 cgggagcggt ggcgcattaa tctctttcgc tgtgtcggta aggatcctga tcggggctac
     2461 gtcacctggc gcccgacgtt tacgacggag ccaagctttc acgcacctga agcttttggc
     2521 tggctggtat tcggcgaatg agcggct
//
LOCUS       DASUEX010000614         2707 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1362889, whole genome shotgun sequence.
ACCESSION   DASUEX010000614 DASUEX010000000
VERSION     DASUEX010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2707
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1362889"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..607)
                     /locus_tag="VFV61_10220"
     CDS             complement(<1..607)
                     /locus_tag="VFV61_10220"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX4900898.1"
                     /translation="MSQAMPQGYETLLLERRGRVALITINRPEKRNALNIKTREDGAA
                     LLAELDRDDSVGVVVITGAGDKAFIAGADIAEFAGRTAMMQRDVMTGQSLFTAMDRFS
                     KPIIAMVNGYCLGGGCELALACDLRIASETASFGQPEINLGIIPGGGGTQRLTRLVGE
                     GKAMELILTGDIIDARTAFAIGLVNHIVPAAELEAKTMEVAS"
     gene            complement(803..1189)
                     /locus_tag="VFV61_10225"
     CDS             complement(803..1189)
                     /locus_tag="VFV61_10225"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX4900899.1"
                     /translation="MSTPYNNEERRFALRMSLRLPIVVSGRAEDGSAWSEPTETDDIS
                     NTGTLFHLNQKVNRGERLYIRAHRPDGAPIEVTAVVVRVAPAIYGTARVGVQIAEPNE
                     NWVRLFVSWVADEQPVISDPDAPPTE"
     gene            complement(1217..2173)
                     /locus_tag="VFV61_10230"
     CDS             complement(1217..2173)
                     /locus_tag="VFV61_10230"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HEX4900900.1"
                     /translation="MACESTTQLATRRFWQNLKEGPPQKIASIAVIGAGTMGHGIAQV
                     AAAAGYRVTLRDLDRDSLARGIRAIERNLDKGMQLGKLSEEDREQTLQNIHGTINLDQ
                     VAEADLIIEAAPEQLKLKQDLLREMESFGERDFIFASNTSSLSISDIATASARPARVV
                     GMHFFNPVHIMRLLEIVVGKATSTETVETVCEVGRRLRKDPIIVNDVPGFASSRLGVA
                     LGLEAMRLLEQGVASAKDIDKAMELGYNHPMGPLKLTDVVGLDVRLNIAEYLHRALGS
                     ETFRPPDVLRRLVSEGKLGKKSGEGFYSWREENGDALQAHSS"
     gene            2300..>2707
                     /locus_tag="VFV61_10235"
     CDS             2300..>2707
                     /locus_tag="VFV61_10235"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX4900901.1"
                     /translation="MPKQTLHRDVVIIGSGPAGLTAAIYTARANLAPLLIDAPADAEK
                     HTVPGGQLMITTDVENYPGFAAGIQGPDLMAEFRKQAERFGTEFLEAWITKVDLQERP
                     FTLHTADFTIKAATLIIASGASAKWLGIPGEAKA"
BASE COUNT          623 a          764 c          708 g          612 t
ORIGIN      
        1 gactagcaac ctccatcgtc tttgcttcaa gttcagcggc gggcacgatg tgattaacca
       61 aaccaatggc gaatgcagtt ctcgcgtcga taatatcgcc cgtcaaaatc aactccatcg
      121 ctttcccttc gccgaccagc ctggtcaaac gttgcgtgcc gccgccgccc ggtatgattc
      181 ccagattaat ctccggctgt ccgaaggaag ccgtttcgct cgcaatgcgc aggtcgcaag
      241 cgagggcgag ctcacaacca ccgccgaggc aatagccatt aaccatcgct ataatcggct
      301 tgctaaacct gtccatggca gtaaacaaag actgccctgt catcacatcg cgctgcatca
      361 ttgccgtccg tccggcaaat tcagcaatgt cggcgccggc aataaacgcc ttatccccag
      421 cgccggtgat tacgacgacc cccacagaat cgtcacgatc aagttcggca agcaaggcgg
      481 caccgtcctc acgcgtcttg atattcagag catttctttt ttccggtcgg ttgatggtaa
      541 tgagagcgac gcgcccgcga cgttcaagca aaagagtttc gtaaccttgt ggcatggctt
      601 gcgacatgaa ctggctcctg tttatttaat tgaatctagt gcgagaatcg taaaggctgg
      661 gatgcgaatc gtcaaagtcg taaccacaga ttacgcggaa accacggatc aagagtaata
      721 aatgacagta ttgctccgct tcatccgcga agatctgtgg ctgaaactgc gtccgtgacg
      781 atcttagaat tagagggtgg acctattcgg taggaggggc gtcgggatcc gaaatcacgg
      841 gctgttcatc agcaacccag gagacaaaga gtcgtaccca attctcattg ggttcggcga
      901 tttggacacc aacgcgagcg gttccgtaaa tcgcgggggc cacgcgcacg acaacagccg
      961 tcacttcgat gggcgcgccg tcgggccggt gcgcccggat gtagagacgc tcgccgcgat
     1021 tcaccttttg gttgaggtgg aagagcgtgc cggtattgga aatgtcgtcg gtttctgttg
     1081 gctcactcca tgctgaaccg tcttccgcgc gtccggagac gacgatcggc aaacgcagcg
     1141 acattcgtaa agcaaagcgc cgctcttcgt tgttataagg tgtgctcata gtgggtaaca
     1201 cagtggcgtt atgctctcag gaagagtgcg cctggagcgc atctccattc tcctctcgcc
     1261 aactataaaa accttcaccg cttttcttgc ctaacttgcc ttcgctgacc agccgccgta
     1321 acacgtccgg tggacgaaac gtctccgaac caagcgcacg atgtaaatat tcagcgatat
     1381 ttaagcggac atcaagcccg acaacatcgg ttagcttcag cggccccatc ggatggttgt
     1441 agccgagctc cattgccttg tcgatgtctt tcgcactggc aacaccttgt tccaacagcc
     1501 tcatcgcttc cagcccgagg gctaccccga ggcggctcga ggcgaaacca ggcacatcgt
     1561 taacaatgat cggatccttt cgcaggcggc ggccaacctc gcaaacggtt tccacagtct
     1621 cggttgacgt ggccttgccc accacaattt ccagcaaacg cattatgtga actgggttaa
     1681 agaagtgcat cccgactact cgtgccggac gcgccgaagc ggtggcaatg tcggatatgg
     1741 aaagcgatga agtgttggac gcaaaaatga agtcacgctc cccgaacgat tccatttcgc
     1801 gcagcaagtc ctgttttagt tttaactgct ctggggctgc ctcgataatt aagtcagctt
     1861 ccgcaacttg atcgaggttg atggtgccgt gaatgttttg aagcgtttgc tcgcgatcct
     1921 cttcagacag tttgcccagc tgcatccctt tgtcgaggtt gcgttcgatt gccctgatcc
     1981 cacgggcgag gctatctcga tcgaggtcgc ggagggtgac tcgataaccg gcggctgccg
     2041 caacctgcgc gataccatgc cccatggtgc ccgctccgat tacggctatg gaagcaatct
     2101 tctggggagg accttccttc aaattttgcc agaatctcct ggtggcgagt tgcgtggttg
     2161 attcgcaagc caatagttcg actacttaag taccagtagt tatactatac gcctaaccga
     2221 aaaggctata ctgatgtgca tttagtcacc gtctgcctta cgattttcac cgctaaacga
     2281 agggaaaaca ccacacacaa tgccgaaaca gacactccac cgggacgtgg taattattgg
     2341 ctccgggccc gccggattga cggccgcaat ctataccgca cgtgccaatc tcgcgccgct
     2401 tttgattgat gctcccgctg atgctgagaa gcataccgtg cccggtggcc agttaatgat
     2461 cacaacggac gtggaaaact accccggatt cgcagccgga atacaggggc ccgatctgat
     2521 ggctgagttt cgcaagcagg cagaacggtt tggcactgag tttctcgaag catggattac
     2581 caaagtcgat ctgcaggagc gacctttcac gcttcacacg gctgatttca caatcaaagc
     2641 cgcaactttg atcattgctt ccggagcatc agcaaagtgg ctgggcattc ccggcgaggc
     2701 gaaggct
//
LOCUS       DASUEX010000615         2950 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_445621, whole genome shotgun sequence.
ACCESSION   DASUEX010000615 DASUEX010000000
VERSION     DASUEX010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2950
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445621"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            297..2027
                     /locus_tag="VFV61_10240"
     CDS             297..2027
                     /locus_tag="VFV61_10240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900902.1"
                     /translation="MPAPTLDAALDALLDRLAGMRRFEPGDSNRVQKLLAQLGRRRFT
                     GSTALIRFHELLLFFRAYPHTPAILQKSEQLLASFHQRVNSLRDSGADVSAFADPPVS
                     GIAGTAFSAIWGYDIVRDLAARYPSRVEIDWEGYEGEELLVAVLKNFLPLFEDGAYVV
                     YPVPYLAWIRAAKKQNETDLAWLLRQFEQLSITERAKANLFDSLKLWTHWKLGNSLVT
                     RTKMRVRVNKIFYHDGPLIRRSEVSLAREIENPLPLPLKKLSRADGEKLVSLGRDMMT
                     VRYRELHGFTYGDPRHVVRATAGRGVEFVIWGLPPGRRLPLLGYHALLILKNGVPSGY
                     AESLALFERTEVGVNLFYTFRDGESAWVYARLLRFLRQYLKVSVFSVEPYQLGSHNTE
                     GVAAGAFWFYRKLGFRPIEPSVASKVAREERKLAARPGYRTSPRTLRQLAEGHLLYEA
                     PSAPRPGEWDNFRVPNIGLAVQRRLVQQAGGDERKARQVLVTSVARALGVNPTEMSAS
                     EHQVFSDLAMVLAMIPDLSRWSNEEKSAIRQIVRAKAGTDELRYVRLLQRHQKLRAGL
                     RKIGSRDETV"
     gene            complement(2067..2768)
                     /locus_tag="VFV61_10245"
     CDS             complement(2067..2768)
                     /locus_tag="VFV61_10245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900903.1"
                     /translation="MTTAGSWIRIGSGALMLVLCMTLQLSAQEAGKASVSFFYPAQLA
                     RGQTTRVTVDGNFDSIQAVELTPAEGVTIQKIEPDATYEGRKPQKRWHVEFLVAADAK
                     PGERSVVFVTPQGPTPPRKVLLPSHVPVLSNFKALTVERTPIRIVFSINVFDAEDDLE
                     SLMFTNSLKCGGSVAIGYSVVGEMKQLAHGKYALQATFNHKQSEALPATCQFSLALDD
                     KNHYEGRLTVPVEFK"
BASE COUNT          614 a          834 c          839 g          663 t
ORIGIN      
        1 cctccaccca ctaatatcgc taagagtcct tttgctgaca tacgcgttca cactgattga
       61 ctgaggcaat cttacgatca cccaaccaga attccacagc ttgggtggtt ttatcgtcag
      121 ccaaaacgcg agctgtggtt agtgtcctgt ttctgaagtt gactgaaaaa ctacgattgg
      181 tgatcagcct gcggagactg aagagccgag tttaggccaa tatcaatcgt tgactctgcg
      241 ggcgtcgtaa catagaaact tagcgtcgga tcagaaacgg agtgcctgga gagaccatgc
      301 ccgcccccac ccttgacgct gcacttgacg ctctgctgga tcggctcgcg ggaatgcgcc
      361 gctttgagcc aggcgacagc aatcgcgtgc agaagctctt ggcccaactg ggccggcgcc
      421 gtttcaccgg ttccaccgcg ctaattcgct ttcacgaact cctgctgttc ttccgtgctt
      481 acccgcacac tcccgcaata ctgcaaaaga gcgaacaact tctcgcctct ttccaccagc
      541 gcgtcaacag tctgcgcgac tcgggtgcag acgtctcggc ctttgccgac ccgccagtct
      601 caggcatcgc gggcacagcc ttctcagcaa tctggggcta cgacatcgtg cgagacctgg
      661 cggcgcgtta cccctcgcga gtcgaaattg actgggaggg ttacgagggc gaagagcttt
      721 tggtcgcagt gctgaagaat ttcttgccgc tgttcgaaga cggtgcgtac gttgtttatc
      781 cagtccctta tctggcttgg attcgcgccg ctaaaaaaca gaacgagacg gacctcgcgt
      841 ggctcctacg ccagttcgaa cagctctcga taaccgagag agcgaaagcc aatcttttcg
      901 attcgctcaa gctctggacc cactggaaac ttggtaactc cctggtgaca cgaacgaaga
      961 tgcgggtccg agtgaacaag atcttctatc acgatgggcc gctcatcagg cgtagcgagg
     1021 tttcactcgc gagggagatt gagaatccgt tgccgctacc gctcaagaaa ctttcgcgcg
     1081 ccgacggcga gaaactggtg agcctgggcc gagacatgat gaccgtgcgc tatcgcgagc
     1141 ttcatggctt cacctatggc gacccgcgtc acgttgtccg cgcgactgca ggccgcggtg
     1201 tcgaattcgt tatctggggt ttgccgccgg gacgccgtct gcctttgctt ggctatcatg
     1261 ccctgttgat cctcaagaac ggcgttccgt caggttatgc tgaatccctt gcgctgtttg
     1321 aacgcacgga ggtaggcgtg aatctcttct acaccttccg cgacggtgaa tcagcgtggg
     1381 tctacgctcg tctgcttcgg ttcctgcggc aatatctcaa ggtctcagta ttctcagtcg
     1441 agccttatca actcggtagc cataacacgg aaggtgtcgc cgccggagcg ttctggttct
     1501 atcgaaaact cggcttccgc ccgatcgaac cgagcgtggc cagtaaggtt gcgcgggaag
     1561 aacgcaaact cgctgcccgg cccgggtatc ggacttcacc gcgcacgttg cgtcaactcg
     1621 ccgagggtca tcttctctac gaagcaccct cggctccacg gcccggtgag tgggacaatt
     1681 tccgcgttcc gaacatcggg ctcgcggttc agcgccggct ggtccaacag gccggcggtg
     1741 acgaacgaaa ggctcgccag gtgctcgtga cgtcggtcgc ccgcgcactt ggggttaacc
     1801 caaccgagat gagcgcgtca gagcatcagg tcttctctga tctcgcgatg gttctggcaa
     1861 tgattccgga tctgtcgcgc tggtcaaacg aggaaaagag cgcaatcagg cagattgtcc
     1921 gagctaaggc aggaactgat gaactgcggt atgtgagatt gctacagcgc caccagaaac
     1981 tccgcgctgg tctgcgaaag atcggctctc gtgatgagac cgtgtagttg tcgagctagc
     2041 aggcggtgaa gacaagagct tagtggctat ttgaattcga cgggcacagt taatctgccc
     2101 tcgtaatgat tcttgtcgtc caatgctaga gaaaactggc aggttgctgg aagggcttcc
     2161 gactgcttgt gattgaacgt tgcttgaaga gcgtacttgc catgcgcgag ctgcttcatc
     2221 tctccgacga cgctatagcc aatggcaacg ctgccgccac acttcaacga attagtaaac
     2281 atcagtgact ccaggtcgtc ttcggcatca aaaacgttaa tgctaaagac gatcctaatc
     2341 ggcgtccgct cgacggtcag agccttgaag ttagagagaa ccgggacatg ggaaggaagc
     2401 aatacttttc tgggtggtgt cgggccctgc ggtgtcacga agaccacgga ccgttcaccc
     2461 ggtttcgcgt ccgccgcaac caggaactcc acgtgccacc gcttttgtgg ttttcggccc
     2521 tcgtacgtgg cgtcaggctc gattttctgg atggtaactc cttcagcggg cgtgagctct
     2581 acggcttgga tgctatcgaa gttaccgtcg acggtaaccc gtgtggtctg gccgcgggcc
     2641 aattgagctg ggtagaaaaa actgacggag gctttgccag cttcttgcgc cgaaagttgt
     2701 agcgtcatgc acaggacaag catgagtgcg ccagacccaa tccgaatcca gctcccggct
     2761 gtcgtcataa aatctcctgg cggaaagccc catactgcat cggcatttcc ttttcgggtt
     2821 ggaagatcgc gcgcattcta tcggcggtgg ctggttgcgg caaagtgaat ttcgtcgtgc
     2881 gagggagaag atccgaggtt gtccactggc gcgaagctat ggctcttcac gtacctgctg
     2941 agctgcatgc
//
LOCUS       DASUEX010000616         3185 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1020730, whole genome shotgun sequence.
ACCESSION   DASUEX010000616 DASUEX010000000
VERSION     DASUEX010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3185
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1020730"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="VFV61_10250"
     CDS             complement(<1..281)
                     /locus_tag="VFV61_10250"
                     /inference="COORDINATES: protein motif:HMM:NF017394.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HEX4900904.1"
                     /translation="MNFPPKARLATLCLLLLIGCETNKVEPVALAPEDMCDYCRMIIS
                     ERRYAAELIDSEGKAFKFDDIGCMANFIKSKRSTTKVVAHFVMDFDSRQ"
     gene            complement(282..1196)
                     /locus_tag="VFV61_10255"
     CDS             complement(282..1196)
                     /locus_tag="VFV61_10255"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4900905.1"
                     /translation="MTIQVRKLTKKFKDVVAVNDVSFDVERGETFALLGPNGSGKTTT
                     LKCLVGLTLPTAGNITVESFDVLRHGREAKRLMSFLPQRLSFSDQLTGREVLEFFCRL
                     RRIPSQRIDETLNTPNFHFNGFFDRSVSQFSGGMTQRLGLAVACLPDTPVLVLDEPTV
                     SLDPNGAIEFREFLTSLKGKGKTIVFSSHVLADVEQLADRVGIMVGGRLVALRSITDL
                     RAELLLSARMRITIEKFDSRLLEAAKRAGASEVKLEGDSLVLKSRGEDRLQILRAIEA
                     VGCCIASFGTEELSLEDIYMNYVNEDVH"
     gene            complement(1196..2575)
                     /locus_tag="VFV61_10260"
     CDS             complement(1196..2575)
                     /locus_tag="VFV61_10260"
                     /inference="COORDINATES: protein motif:HMM:TIGR04247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
                     /protein_id="HEX4900906.1"
                     /translation="MRSRPLSRVLLIPLALVLVTVSARGETFTVGGSDAQFQSIQEAL
                     NAAHDGDLIKVRAGTYVGNLTLNKQIKLEGIDRPTLRGEGVASVITVLGNGCVISGFI
                     IEHSGRDLTREDSGILLRSSNNQIEDNELRDILYGIYLYNSHGNTVRRNRVRGRPELE
                     EGERGAGLHLWSSADNTIEENIISEERDGMYIQSSHGNQIRRNRVFNLRYGLHYMSSD
                     RNVFEDNLFSNNVAGAAIMYSNHIAFRRNAFIHNRGFSSFGILFQDCSDLLAEENFII
                     DNATGIFMEALRNTTFRHNTIANNDVALQLFSSSDANVFTENNFIDNLSPLRLIGKST
                     TTRWSENGRGNYWSDYEGYDLNEDGRGDVPLKIQNVFEYMEGNHPRLRLYLDSPAARA
                     IAVAEKSFPILRGSTEMDPAPLMKPVQFLYPAALERPAARLHAGLMIASLLILTTAAM
                     AIWRGQKLW"
     gene            complement(2589..>3185)
                     /locus_tag="VFV61_10265"
     CDS             complement(2589..>3185)
                     /locus_tag="VFV61_10265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900907.1"
                     /translation="VLILVAVVALLPSFFFPLWNMSFYSNQYTDGLVLHIYAYQLEGG
                     KTPNRDDLREINSLNHYIGMRPLLESDFSEFTWVPLVIGLLMILALRAMVLGKMSNLV
                     DVFVLFTYFGLFSMWSFYHRLYMYGHNLDPTASIKVEPFTPPFFGTKQIANFAVNSFP
                     GWGSYAFLAFAILMLLAILLSAKRGRLKDQQTVSAGVA"
BASE COUNT          777 a          859 c          787 g          762 t
ORIGIN      
        1 cattgacgtg aatcgaagtc cattacaaag tgcgcgacta ctttcgtcgt gctcctttta
       61 cttttaataa agttggccat gcaaccgatg tcatcgaact tgaatgcctt cccttcgctg
      121 tctatcaatt cagccgcata acgcctttct gaaattatca tcctgcagta atcgcacatg
      181 tcctcaggag ctagggcgac cggttctact ttgttcgttt cacaaccgat cagcagaagc
      241 agacacaacg tcgccagtcg cgctttcggc ggaaagttca tctaatgaac gtcctcattc
      301 acatagttca tataaatatc ttccaacgac aactcttcag tgccgaaact cgcaatgcaa
      361 catccaacgg cttctattgc tcgcaaaatc tgcaggcgat cttccccgcg agacttcagc
      421 accagtgaat ctccttcgag cttcacctcg ctcgctccgg cacgcttagc tgcctcgagc
      481 aaacgcgaat caaacttctc gattgtgatg cgcattcgag cactcagcaa taactcggct
      541 cgtaggtccg taatggaccg cagggcaacg agtcgtcctc cgaccatgat cccgactcgg
      601 tcggccaact gctcaacatc tgccagcacg tgcgaagaga acacaatcgt cttaccttta
      661 cctttaagcg aagtaagaaa ctcgcgaaac tcaattgcgc cattggggtc gaggctgaca
      721 gtcggctcat ccaaaaccag caccggtgta tcgggcaggc aagctaccgc cagtcccagc
      781 cgctgggtca tgccccctga gaattggctg actgatctgt caaagaaacc gttgaaatgg
      841 aagttcggcg tgtttaatgt ctcatcgatt cgttgagacg gtattcgccg caagcgacag
      901 aagaactcca ggacctctct tccggttaac tgatcgctga agctaaggcg ctgcggaagg
      961 aagctcatca atcgcttagc ttcacgacca tgacgcaaga cgtcgaagct ttcgaccgta
     1021 atgtttccgg cggttggcaa agttaatccg accaggcatt tcaaggtagt ggttttgccg
     1081 cttccgtttg gtccgagcag ggcgaacgtc tcgccgcgtt cgacgtcgaa cgagacatcg
     1141 ttgacggcca cgacgtcttt gaacttctta gtaagttttc tgacttgaat agtcattacc
     1201 agagcttctg tcctctccag atcgccatcg ccgccgtagt tagaatcaat agcgaggcga
     1261 tcatcaagcc ggcatggaga cgagccgcgg gccgctccaa cgccgccggg taaaggaatt
     1321 gcacaggttt catcagtggt gccggatcca tttccgttga tcccctcaag attgggaacg
     1381 atttttcagc cactgcgatc gcccgagcag caggactgtc aagataaagg cgtaagcgcg
     1441 gatggttgcc ttccatgtac tcgaagacgt tctgaatctt gagcggcaca tcgccacgtc
     1501 cgtcttcgtt caaatcgtaa ccctcatagt cgctccaata attcccgcgt ccgttctcag
     1561 accatctggt ggtggtgctt ttgccgataa gacgcagcgg actcaagttg tcaatgaaat
     1621 tattctcggt gaagacgtta gcgtccgagc tgctgaacaa ctgcaaggcg acatcgttgt
     1681 tcgcaattgt gttatggcgg aaggtcgtgt tccgcagcgc ttccatgaaa atgcccgttg
     1741 cgttgtcgat gatgaagttc tcttcggcca gcagatcact gcagtcctga aaaaggatgc
     1801 cgaaagagct gaatccgcga ttgtgaataa aagcattgcg gcgaaacgcg atgtgattgg
     1861 agtacatgat cgccgcaccg gccacgttat ttgagaacag attgtcttca aaaacattcc
     1921 tgtccgagga catgtaatgc aaaccgtagc gcaagttgaa aacacggttg cgcctgatct
     1981 gattaccatg ggaactttgg atgtacatcc catcgcgctc ttcggaaata atgttctcct
     2041 cgatcgtgtt gtccgcggag ctccagaggt gcagtccggc gccgcgctct ccttcttcga
     2101 gttcaggtct gccgcgcacg cgattacgcc gcacggtatt gccatgtgaa ttgtagagat
     2161 agattccgta gagtatgtca cgcagctcgt tgtcttcgat ttggttgttg ctcgatctga
     2221 gtaagatgcc ggaatcttct cgtgttagat cacgaccgct gtgctcgata atgaagccgc
     2281 taatgacgca cccattaccg agcacagtta tcacgctggc gacaccttca ccccgcaagg
     2341 tgggacggtc tattccttca agcttgattt gtttattcag cgtgaggtta cctacgtagg
     2401 ttccagcgcg gactttgatc agatctccat cgtgcgcagc attcagggct tcttgaatcg
     2461 attggaattg tgcatcgctc ccgccaaccg taaacgtttc gccacgcgcg gatactgtca
     2521 ccaggaccag cgcgagggga atcaacagta ctcttgataa aggtcggctt ctcattgaac
     2581 cccttaactt aggcaactcc tgccgataca gtctgctggt ctttaagccg acctcgttta
     2641 gcggacaaca aaatcgctaa cagcatcaag atcgcgaacg caagaaatgc gtaggaaccc
     2701 catccgggaa aactattaac tgcaaagttc gcgatctgtt tcgtcccaaa gaacggcggt
     2761 gtgaagggct caaccttgat cgatgctgtg ggatccaggt tgtgcccata catatagagc
     2821 cggtgataga agctccacat cgagaacaag ccgaaataag tgaagagcac aaacacgtcg
     2881 accaggttag acatcttccc gaggaccatc gctctcagcg ccaggatcat tagaagcccg
     2941 atcaccagtg gcacccatgt gaattcgctg aagtcactct caagcagtgg ccgcatgccg
     3001 atgtagtgat taagcgagtt gatttccctc aggtcgtcgc ggttaggtgt tttcccgcct
     3061 tccaactgat acgcatagat gtgcaggacc agcccgtcgg tatactgatt tgagtagaag
     3121 ctcatgttcc agagtggaaa gaagaaagac ggaagaagag ccacgactgc gaccagaatc
     3181 agaac
//
LOCUS       DASUEX010000617         2824 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1021313, whole genome shotgun sequence.
ACCESSION   DASUEX010000617 DASUEX010000000
VERSION     DASUEX010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2824
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1021313"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="VFV61_10270"
     CDS             <1..294
                     /locus_tag="VFV61_10270"
                     /inference="COORDINATES: protein motif:HMM:NF014856.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide synthetase C
                     domain-containing protein"
                     /protein_id="HEX4900908.1"
                     /translation="VLASHGYPGKYEKGKVIQGLQAPAAQATVFHAGTDRLASGELVT
                     DGGRVLGITAQGETLPEALRRCYNAIQNISWDGMQFRRDIGQFREVRSKTSIP"
     gene            544..1305
                     /locus_tag="VFV61_10275"
     CDS             544..1305
                     /locus_tag="VFV61_10275"
                     /inference="COORDINATES: protein motif:HMM:NF015945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HEX4900909.1"
                     /translation="MGLWTRVKRSMRALFGGIIEATENPELVLQQTIRDMRDRVPELN
                     NSVAQVMATEKLLGKSKERLESQVVDLDSKIKASVKMGRDDIATAYIGQLQQAQMDLQ
                     KTGQQLEHATLASKQALKARDNYVLQMQRRTAEAMQLINQSKQARLQEQLAQTMESFQ
                     IGDDASTFNEMRDKIDRRAAAAEAKMQLGSASVDNQMQDIEREAMDMQLQDKLLAYKR
                     EMGLLPAGTPASPQALPAEGQSSANDNAGQGNRGN"
     gene            1351..2190
                     /locus_tag="VFV61_10280"
     CDS             1351..2190
                     /locus_tag="VFV61_10280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900910.1"
                     /translation="MAEFSKFDLRYIKEAFKEPLNFWGIITFGAAAAFFQDVTPLAAA
                     LATETLYLATVPASSFYRRLVDRREKQRLHKLREQQRESQIKGFDPREREAVEYLRWM
                     KNQIYSNYKKFTGSKTIPHNLESLDQRWEDFVDLLDVYRRRKHHLRSINRQAVQNQLV
                     QAERSVQHSKDEREKRIQQSNVEILRRRVAAFQDIERSVKLVEGQLQSIENFFGLVND
                     QVVTLPTPERVSALDFEQLSDSIAMTKQMLEETSDTFAALDSHNRDIGNYDLLLSSSS
                     PSK"
BASE COUNT          752 a          736 c          754 g          582 t
ORIGIN      
        1 gtactggcca gtcacggcta tcccgggaaa tacgaaaagg gaaaagtaat tcaaggtctt
       61 caagctccgg ctgcccaagc cactgtgttc cacgcgggaa cggacagatt agcgagcggc
      121 gaattagtta ccgatggagg acgtgttctg ggaatcacag cgcagggcga gactctcccc
      181 gaagccctga gacgttgcta caacgccatc cagaatatct cctgggatgg catgcagttt
      241 cgtcgcgata ttgggcaatt cagggaagtc agaagcaaaa cgagtattcc ctaaggttgg
      301 ttgtaaccca cactgttagt ttgcttcctg agggttaact gctggctcag gattacgacc
      361 ctaagccgca ctttctggag caaggctgga actactgcta tactccggac tcgcttccta
      421 cggtattttg aaccctattg cggccgccaa cgtactgaca tatgcggacg ggcagaagtt
      481 aatctttaac gccaaatcga agctatcgac ccctacttct gattctggag gctgagttaa
      541 aaaatgggac tgtggaccag agttaaacgc tcaatgcgcg ctttgtttgg cgggatcatc
      601 gaggcgactg agaatcccga actcgtactc cagcaaacca tcagagatat gcgcgaccgt
      661 gtacctgagc tgaacaactc ggtggcacag gtgatggcta ccgaaaagct gctcggcaag
      721 agtaaggagc gcctcgaaag tcaggtcgtg gacctcgatt caaagattaa agcgtcggta
      781 aagatggggc gtgacgacat cgcgacggcc tacatcggcc agttacagca agcgcagatg
      841 gacttgcaga aaaccggtca gcaactggag cacgccacgc tcgcatccaa gcaggcgctc
      901 aaggcgcgag acaattatgt cttgcaaatg cagcgtcgca ccgccgaggc gatgcagcta
      961 atcaaccagt cgaagcaggc gcgcttacaa gagcaactgg cgcaaacgat ggagtcgttc
     1021 caaattggtg acgacgcttc cacgttcaac gaaatgcgcg acaagattga ccggcgtgcc
     1081 gcagccgcag aggcgaaaat gcagctgggg tctgcttcgg tcgacaacca gatgcaggac
     1141 atcgagcgcg aggccatgga catgcagtta caagataaac tgctcgccta taagcgggag
     1201 atgggattat tgcccgctgg gacgccggca tctccgcagg cgttgcccgc cgaaggccaa
     1261 agcagcgcca acgacaacgc cgggcagggc aatcgaggta actgatcgcg ggtgggtcac
     1321 gatggcttac ctctttaagg cgtcaagttt atggctgagt tttcgaagtt tgacctgcgg
     1381 tacataaaag aggcttttaa agagcctctc aacttttggg gcatcattac attcggtgcg
     1441 gcagcagcat tttttcagga tgtcacgcct ctcgctgcgg ccttagctac cgagactctc
     1501 tatctagcca ccgtgccggc aagttcattt tatcgacgtc tggttgaccg tcgcgagaaa
     1561 cagcgactgc ataagctgcg cgaacagcag cgcgagtctc aaatcaaggg attcgatccg
     1621 cgtgaacgtg aggcggtcga gtacctgcga tggatgaaga accagattta ctcgaactac
     1681 aagaagttca ccggctcgaa aacaatccct cataatctgg aaagtctgga tcagcgttgg
     1741 gaagattttg tggacttgtt ggatgtttat cgccgtcgca agcaccacct cagatcgatc
     1801 aaccgccagg ccgttcagaa tcaactggtg caggctgagc gttcagtgca acattccaag
     1861 gacgaacgtg agaaacggat tcagcaatca aacgtggaaa tcctgcgccg gcgcgtcgcc
     1921 gctttccagg acattgaacg ttccgtgaag ctcgtcgaag ggcagttgca gtcaattgaa
     1981 aacttcttcg gactggttaa tgaccaggtc gtgactctac ccacaccgga gcgcgtttcg
     2041 gcgctggatt tcgaacagct tagtgattca atcgcaatga cgaaacaaat gctggaggag
     2101 acttcggaca ccttcgccgc gcttgatagt cacaaccgtg atatcgggaa ttacgacctc
     2161 ctactctcca gcagctcgcc ctcaaaatag caaggccggc tcctctctcc tcccaaaaaa
     2221 cccgacgcga ttgatgccgg aattgaagca aacttctgga gtccaggtgt cttttgagaa
     2281 tgatccccgc caagggtctt gaggggaggg agctagcgga tttgatcacc agagaattac
     2341 aaaaataatc cagcaagacc gggaaaagag aggcgggatt gaacctgatt ttgggagcgc
     2401 tctgacagtc aaaccgaccg ccaatcagta ctttagtagt taacgtagag tcttaattta
     2461 cacactataa tgcattgaaa aaaaagatga ccacaacata ttgacatgcg gtaatcaaag
     2521 gagtatattc cgctccgctt gagacgaggc gggtaacttg gagcagcggc aggcccccct
     2581 gaacggctct ccagctaaag tttttcgctt cgaaaaaagc caaccgaagt atccgctaaa
     2641 catgattgac gcagccacgt ctactataat gtagccggag gttcgtcttt gaaacttccc
     2701 gaaaatcacc tcgtgtctcc cgaacacgag aaaggcagaa cgatgagcgc agccattggc
     2761 aaaaacaccc cttccgccga cgtgtcgtct aaaacacccg caacgccggt caaacgtcgc
     2821 aaga
//
LOCUS       DASUEX010000618         3159 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_793546, whole genome shotgun sequence.
ACCESSION   DASUEX010000618 DASUEX010000000
VERSION     DASUEX010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3159
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_793546"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            375..581
                     /locus_tag="VFV61_10285"
     CDS             375..581
                     /locus_tag="VFV61_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEX4900911.1"
                     /translation="MSRITGKVKWFNNSKGYGFIEQPGSSDIFVHYSAIQGDGFKTLE
                     EGQEVEFEVTQGPKGPQAEQVTKI"
     gene            complement(699..1337)
                     /locus_tag="VFV61_10290"
     CDS             complement(699..1337)
                     /locus_tag="VFV61_10290"
                     /inference="COORDINATES: protein motif:HMM:NF041621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MXAN_5187 C-terminal domain-containing protein"
                     /protein_id="HEX4900912.1"
                     /translation="MRIENRLQRAQLRARSKNGFGGDSQITIDDQLTRLEEDIRRLKI
                     EFDIYFNGASKRPPYDTKGRVETILKRLGDDRSLTFAERYRFNTLMARYNVFRDLWRR
                     TMQGREEGKDPVSAARATAKREAVESFTKTTLVCLDASREVETVKNLYDALVDAKTKC
                     GESTEDFSFPRFHRLIASKAESLKARLKCERVSFSVDVEGGHVSFKARAERD"
     gene            1478..2899
                     /gene="selA"
                     /locus_tag="VFV61_10295"
     CDS             1478..2899
                     /gene="selA"
                     /locus_tag="VFV61_10295"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HEX4900913.1"
                     /translation="MSEPAKLSALRELPSVDQLLKTEAAGRLREVSGAERLTTIAREV
                     TDELRSEILANPKNADVRTSRETLLMQAIQRMESRVMIEENAGLRHVINATGVILHTN
                     LGRAPLSLEARSALTQDASGYCTLEYDSATGTRGRRGARVEQLLVELTGAEAALVVNN
                     CAAAALLILSVLAHDGETIVSRGELVEIGGDFRVPDVMANSGTRMVDIGTTNRTHLED
                     YRAAINARTRLLMRVHPSNYRVVGFTTAPTLAELASLADEKGLILYEDAGSGVLQDLS
                     AYGLKGEPIIRDSLAAGADVVSFSGDKLLGGPQAGLIVGSLEIIERLRKHPLYRALRV
                     DKLTLAALQATLNSHRRGTLFDEIPALQMLVLTKSDVAQRAENLVTNLGHNKADSDLS
                     ITLIDGESAVGGGSGPNTKPPTALIALQHARYSPDEIERRLRCFQPPVIARVAEAKVL
                     LDLRTVAPSEEPELIRAIQSLAS"
BASE COUNT          690 a          880 c          837 g          752 t
ORIGIN      
        1 gggtttaggt ttatgctttt atttccttga caccctgaag aattttcgct tagtattcgc
       61 cgtcgtccca atgcagggtt ttcctttcga gattttccca gcattccgat ctgccagctt
      121 tacagcaatg agttttagta gtttcgcccc cgcaagtgta tgcatgctga aatcgcgctc
      181 tacgcgtttt caccatgcat tttttgttaa ttgtgtgttt cgcgtagcgc ccgcaaggcg
      241 tatcccggct cacaggaatt gacgtcctcg gcgcggagta gcacagagcc agtctgtagt
      301 ttcgcaagaa caaacagcgc aagacttgta ctaatctcgc ccgttttacc gaatcgttag
      361 gaggattcgt tgttatgtct agaataaccg gtaaggtcaa gtggtttaac aattcgaagg
      421 gctatggctt catcgaacag ccaggctctt cagacatctt cgttcactac agcgctattc
      481 agggcgacgg cttcaagact ctcgaagagg gtcaggaagt tgaattcgaa gtgacgcagg
      541 gtccgaaagg accgcaggct gaacaagtga ccaagatata gtctcccccg gcttgcaaca
      601 gcaaagccac tgactgcttc cgatccaatg atccggtaag cgaaaaagaa agaggcgacc
      661 ccaagggtcg cctctttgat ttggccagga tgccctcctc aatctcgttc agcgcgtgcc
      721 ttaaaactga cgtgtccgcc ttcaacatcg accgaaaagc tgacgcgttc acatttcaag
      781 cgtgccttta gactctccgc cttagaagct atcaggcgat gaaatctggg aaacgagaaa
      841 tcttcagtag attcaccaca ctttgtcttg gcgtcgacca gtgcatcgta aaggttcttt
      901 actgtttcta cctcgcggct cgcgtcgaga cagacgagcg tcgtcttagt gaaactctca
      961 acagcttcgc gtttggcggt tgctcgagca gccgagactg gatccttgcc ctcttccctg
     1021 ccttgcatgg tccggcgcca aagatcgcgg aaaacgttgt atctcgccat caacgtattg
     1081 aaccggtagc gctcggcaaa ggttaacgag cggtcatcgc ccagacgctt caggatcgtc
     1141 tcgacacgac ccttcgtgtc atacggcggc cgcttggacg cgccgttgaa gtagatgtca
     1201 aactcgattt tgaggcggcg aatgtcttct tcgagccggg ttaactggtc atcaatggtg
     1261 atctggctgt cgccgccaaa gccgttcttg cttctggccc ttaattgggc cctttgcagc
     1321 ctgttctcta tacgcaaggg gttgctcctg aaggagtgat ttgaggcggg gtgcgtcgat
     1381 gaagacgcta ctgaccgagt ctaggtgttg gaaaaggcag gtgtcaatag aagagttgaa
     1441 ttgctcccga aactcacagc cgttatgcta accgcccatg tctgaaccag ccaagctcag
     1501 tgccctgcgc gaacttccct cggtcgatca gctcctcaag acggaggccg ccggtcgtct
     1561 ccgggaagtc tcaggagcgg aacgtttgac aaccatagcc cgcgaagtca ccgatgaact
     1621 caggtccgag attctcgcga atccaaaaaa tgcggacgtt cggaccagcc gtgagaccct
     1681 tctcatgcaa gcaattcaac ggatggaatc ccgggtaatg attgaggaga acgccggatt
     1741 gcgtcacgta atcaatgcca ccggggtgat cctgcacacc aacctaggtc gcgccccgct
     1801 ttcgctcgaa gcacgctctg cccttactca agacgcgtca ggctactgca ctcttgaata
     1861 cgactcggcg actggaacgc gcggccgtcg tggcgcacgg gtggaacaac tcctggtcga
     1921 actaactggc gcggaagctg cgctcgtggt gaacaactgc gccgccgcgg ctttgctgat
     1981 cctctcggtt ctcgcccacg acggcgaaac gatagtttcg cgcggagagc tggttgaaat
     2041 tggtggcgac ttccgcgttc cggatgtgat ggccaattca ggaacgcgca tggtcgacat
     2101 aggaaccacg aatcgaactc atctcgagga ctatcgcgca gccattaacg cgcgcactcg
     2161 cctgctgatg cgcgtgcacc cctcaaacta tcgcgtggtg ggttttacca cggcaccgac
     2221 attggctgag ttggcaagtc tcgctgacga gaagggattg atcctttatg aagatgccgg
     2281 ctcgggagtc cttcaggatt tgagtgcata cggtctaaag ggcgagccaa tcattcgcga
     2341 cagtcttgca gccggcgctg acgtagtttc cttcagcggt gataaattgc ttggtggacc
     2401 acaggcaggt ctgatcgttg gcagtctgga gattattgag cgcctacgaa agcatcctct
     2461 ctatcgcgca ctgcgtgtcg acaagctcac ccttgcggcc ctgcaagcaa ctctcaattc
     2521 ccatcgacgc ggaacgctct ttgatgaaat tcccgcactg caaatgttgg tgctgacgaa
     2581 gtcggacgtg gcccagcgtg ctgagaacct tgtgacgaac ctggggcaca acaaggccga
     2641 ctctgatctc tcgataactc tgatcgatgg tgaatccgct gttggcggcg gttcggggcc
     2701 taacaccaag ccgccgacag cattaattgc gctgcaacac gccaggtact cgccggacga
     2761 aatcgagcgc cgcctgcgat gtttccagcc cccggtcatc gcccgtgtcg cggaggcaaa
     2821 agtgcttttg gatttacgca cagttgcgcc atccgaagaa ccggaactca tccgggcgat
     2881 acaatccctt gcaagttaaa cccaattcct cggctgaatt aggatttttg tagcctcgcg
     2941 aatctcagcc gggtatactc ttcgctggct ttgttcccca cagagcgcct catcaagccc
     3001 gttggtttgc aaatcccgcc ctaaaccgtg tctgagaaaa actcttctta taaacgttcg
     3061 ctgctgttct tgagcatagc tctgcttgca gcgatggttc tgctggtttc gcgctggctg
     3121 agcgtaggac ccctatcaag ccttactgtt ttcatgtct
//
LOCUS       DASUEX010000619         2926 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1699538, whole genome shotgun sequence.
ACCESSION   DASUEX010000619 DASUEX010000000
VERSION     DASUEX010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2926
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1699538"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1208)
                     /gene="topA"
                     /locus_tag="VFV61_10300"
     CDS             complement(<1..1208)
                     /gene="topA"
                     /locus_tag="VFV61_10300"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEX4900914.1"
                     /translation="MAKNLVIVESPAKAKTINKYLGKDYLVKASIGHIKDLPSKGLGV
                     DVEDDFKPTYEVIPDSRKRNNKRIVAELKKAAKEADAVYLAADPDREGEAICQHLAEE
                     IVPKRPAKPTYRVMFNEITKRAVNEAFEHPKQIDQNLVDSQQARRVLDRLVGYKVSPL
                     LCRTVGGKLSAGRVQSVALRMVVEREREIENFIKTEYWTIFANLGAKLPPAFDARLFK
                     VDEQTVKTSGFDQDIKKNEILISDEAQATEIVAEAEKQSFVVSEVTKKERKRNPVPPF
                     ITSKLQQEAARKLGFAVKRTMMVAQKLYEGIEIGAEGSVGLITYMRTDSTRISDAALG
                     ELRDFVGKQYGAPYLPEKPIHYRSKKDAQDAHEAIRPTDVARTPDSLASFLNNDELRL
                     YRLIWQRTVAS"
     gene            complement(1394..2527)
                     /gene="dprA"
                     /locus_tag="VFV61_10305"
     CDS             complement(1394..2527)
                     /gene="dprA"
                     /locus_tag="VFV61_10305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEX4900915.1"
                     /translation="MKEWVALNMTPGIGPRAAAKLLERFGSAEAVYSATRAELEKLRL
                     LPEAVESIIARDLFVRAEAEIENVRRLGADLLILDDGVYPALLREIYDPPIVIYVKGA
                     WEVCLDRPCVAVVGSRRCSTYGQNAALMLARDLAQRGVTVVSGFARGIDAAAHRGALE
                     GGGRTVAVLGTGMGQYYPRDHKRLADEIIDRGGAIVTQFPLGTPPVAENFPYRNRIIS
                     GLSLGVVVVEAAENSGSLITARLAIEQNREVFAVPGNITSRNSFGTNYLIKGAGAKLV
                     QQWQDIATELPPQIAARLLPPPFGDKKREKSLADQLTLVPAGLSGSETLVLKLLTADT
                     PAQIDTLVERSKLSISDLTAALLALEMRELIRALPGRCFVRKM"
     gene            complement(2593..2926)
                     /locus_tag="VFV61_10310"
                     /pseudo
     CDS             complement(2593..2926)
                     /locus_tag="VFV61_10310"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="four helix bundle protein"
BASE COUNT          636 a          828 c          741 g          721 t
ORIGIN      
        1 gaagctacag tccgttgcca gatcaggcgg tacagccgca gctcatcgtt gtttaagaag
       61 ctggcgagtg agtcgggagt gcgcgcaaca tctgtcggac gaatcgcctc gtgagcgtcc
      121 tgcgcatcct tcttcgagcg ataatgaatc ggcttttctg gtaagtaggg agcgccatac
      181 tgtttgccaa cgaagtctct taactcaccc aaggccgcat cagaaatgcg ggttgaatca
      241 gtacgcatat acgtgatcaa cccgactgag ccttccgcac caatttcaat accctcgtaa
      301 agcttctgag caaccatcat cgttcgcttt acagcgaatc caagtttgcg ggcggcctcc
      361 tgctgtaact tggaagtgat gaaaggcgga actggattac gtttacgctc cttctttgtt
      421 acttcactaa caacgaacga ctgcttttca gcttcggcaa caatttccgt agcctgggcc
      481 tcgtcgctga tcaggatttc gtttttcttg atgtcctgat cgaatccgct cgtctttacg
      541 gtttgctcat caactttgaa aagacgggcg tcaaatgcgg gtggcagttt ggcgccgaga
      601 ttagcgaaga tcgtccagta ctcagtcttg atgaagtttt cgatttcgcg ctctcgctcg
      661 accaccatac gcagcgctac agactgcacg cggccggcgc tgagcttccc gcccacggtg
      721 cgacaaagca gcggtgagac cttgtaacca accaggcgat ccagaacgcg acgtgcctgc
      781 tgagaatcga cgagattttg atcaatctgt ttcgggtgct caaacgcctc attcaccgcg
      841 cgcttggtga tctcattgaa catcacgcga taagttggtt ttgccggacg cttgggaacg
      901 atctcttcag cgagatgttg gcagatcgct tcgccttcgc gatcggggtc ggccgccaga
      961 taaacagcgt cggcttcctt tgcagctttc ttcagctcgg caacgattcg tttattgttt
     1021 cgcttgcgag aatccggaat cacttcatag gtaggtttga agtcgtcctc gacgtccacg
     1081 ccgaggcctt tagaaggcag atctttgata tggcctatcg aggccttaac cagatagtcc
     1141 ttgccgagat atttgttgat cgtcttggcc ttggcgggcg attcaacgat gaccagattc
     1201 tttgccataa ggtactgtta atttctaatt ggcgattgcc gactgataaa tatttccgat
     1261 tgccaattgg cgattgccca ttgatgaata tttccgattg ccaattggcg attgccgatt
     1321 tgttcagctt taagtactaa ccgcttactt tcgattgcgt actcaacatt gactgtctgc
     1381 caatcggcaa tgtctacatt ttcctcacaa agcaccggcc tgggagggcg cgaatcagtt
     1441 cgcgcatctc gagtgccagc agcgccgccg tcagatctga aattgatagc ttgcttctct
     1501 caaccaacgt atcgatctgt gccggcgtgt ccgccgtcaa tagctttaac accaaagttt
     1561 ccgatcctga aagtcccgcc ggaaccagcg tcaactggtc ggccagcgat ttctcccttt
     1621 tcttatcgcc aaaaggaggc ggtaacaatc tcgccgcgat ctgcggcggc aactccgtgg
     1681 caatgtcctg ccactgttgc accagcttgg cgccggctcc tttgatcagg tagttcgtgc
     1741 cgaaagaatt tcgcgaagtg atgttaccgg gcaccgcaaa tacttcccga ttttgctcaa
     1801 tcgcaagccg tgccgttatc aaagatcccg aattttccgc cgcttctacc actaccacgc
     1861 ccaaactcaa gccactaatg atgcggttgc ggtaaggaaa gttttccgcg accggcggcg
     1921 taccgagtgg aaactgcgtg actatcgcac caccgcgatc gatgatctcg tcagcgagtc
     1981 gtttgtgatc acgcgggtag tactggccca ttcccgtgcc cagcacggcc accgtgcgac
     2041 ctccgccctc aagcgcgccg cgatgagcag ccgcatcaat gccccgcgca aaacctgaga
     2101 ccaccgtcac gccccgttgc gcaagatcgc gagccaacat tagcgccgcg ttctgtccgt
     2161 aggttgaaca gcgacgtgaa ccaaccactg ccacgcaggg ccgatcgaga cagacttccc
     2221 aggcgccttt aacgtaaatc acgatcggtg ggtcgtaaat ctcgcgcagc agcgcgggat
     2281 agacgccatc atcaagtatc aaaagatcag cgccgaggcg gcgaacgttt tctatctcgg
     2341 cttccgcccg gacaaacaaa tcgcgagcga tgatgctttc gactgcttcc ggcagcagtc
     2401 gcaatttctc aagctcagct ctggtggcac tgtaaacggc ttcggcggat ccaaagcgct
     2461 ccaaaagctt ggcagcggcg cgaggtccga tgccgggggt catgttgagg gcaacccatt
     2521 ctttcatacg attgcagact ttggagtgag gattgcggat tggactcgtt ccaacccgcg
     2581 gtctgcgtac cgctagtgct gacgctttgc cgttttgatc gaagcaacaa ctatagcgag
     2641 aatctcgtct gcttcgctca tcaagtcggc gattcgtttt ggtttcacaa ttctcgtatc
     2701 aattagtaat tccatccaac agatcgtttc gtctgcttct tcttctaccg ttcccatctt
     2761 ggagacaaaa tcagcccttg attaggctcg gcatgcagca cggtaattgg caccgaccga
     2821 cgaaccgcag cgaatcattt ggtttctgat tactcgcgca gtcggcgtgt ccggcaagca
     2881 ctcggccgag cggaagactc ttaaggcaaa ccggcgtgtc cgattc
//
LOCUS       DASUEX010000620         3180 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1699561, whole genome shotgun sequence.
ACCESSION   DASUEX010000620 DASUEX010000000
VERSION     DASUEX010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3180
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1699561"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..774)
                     /locus_tag="VFV61_10315"
     CDS             complement(<1..774)
                     /locus_tag="VFV61_10315"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HEX4900916.1"
                     /translation="MEIKKVGVLGCGLMGSGIAQVAASAGFPTVVREVSDELIKKGFV
                     GIEKSLAKFAEKGTITADQQKEIRGRLTGTTTFDDLADCDIIIEAIIENIEEKRSTYK
                     QLDQICKPETIFASNTSSISITEMMTATSAGRQRRFVGLHFFNPVPLMKLVEVVRTIL
                     TDEAIYEQAIEFGKRLGKTPVRANDTTGFIVNRLLVPYMLDAIRALEEGVGSIVDIDT
                     SMKLGLGYPMGPFTLGDFVGLDTTYYIAEIMFNEFREKRF"
     gene            complement(826..2124)
                     /locus_tag="VFV61_10320"
     CDS             complement(826..2124)
                     /locus_tag="VFV61_10320"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX4900917.1"
                     /translation="MCRSCGAIVGAGQTTCAVCGASTSAPASPRPTRTVPDRETVKFA
                     RALMERPYKFTIILLVTNFFIYLLMWQSSGMQFSMLGVFPFHVLIAYGAKLNSLITTG
                     HQWWRFVSPMFVHVNLLHLLVNMYSLWIIGPYVEKLYGSSKFVVLWVATGIAGVVASY
                     LTVVSPDAPLGALGRFLFKIHDEPSAGASGALFGLVGVLFVFGIKFRHELPEGFKRAF
                     GIGLLPMIVLNLFIGYVGRGIIDNAAHLGGLTAGAALALVVDYRRPGERSGVAVVWQI
                     LRVLALALVAISFVQVARHFRDPATASIVLPPIQYKTTADSFVVYAKAMNEAQEAFYL
                     AVQEGDSQPIASAVQNLNNAPYLDQKAHELRETMRNLLLEAATVNATEGTTTNADAKK
                     QEKSQLIQRFGVWAKDYNEWLRTVGKKYSDLTDVKPETSQ"
     gene            complement(2279..2836)
                     /locus_tag="VFV61_10325"
     CDS             complement(2279..2836)
                     /locus_tag="VFV61_10325"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX4900918.1"
                     /translation="MADKPFLTDIKTLRERARQHIEKGAITPGYTADLPTVIKLLNEA
                     LATEIVCVLRYRRHYFTASGINAEGVAAEFLQHANEEQGHGDLIAQRIVQLGGEPNFD
                     PEGLLTRSHAEYDDSPGLVDMIKEDLVAERIAIDSYREMINYVGNDDTTTRRMLEGIL
                     AMEEEHADDLVSLLQEIGASKASTA"
     gene            complement(2947..>3180)
                     /locus_tag="VFV61_10330"
     CDS             complement(2947..>3180)
                     /locus_tag="VFV61_10330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900919.1"
                     /translation="KETKQTSNSLKLPILVPFASSAGGDAELRIQVTLFYCREDNTGT
                     CRIKTLVWRAPISVVKDPTASTEIRIQGRVTAE"
BASE COUNT          735 a          875 c          816 g          754 t
ORIGIN      
        1 gaatcgcttc tcacgaaact cattgaacat gatttccgcg atgtagtagg tcgtatcaag
       61 tcccacaaaa tcgccgagtg taaacggacc catcgggtat cccaggccga gtttcatcga
      121 cgtgtcgata tccacaatcg acccgacgcc ttcttcgagc gcgcggatcg catccagcat
      181 gtaaggcacc agcaaacggt tcacgataaa accagtggtg tcgttagcac gcaccggcgt
      241 ctttcccagt cgtttaccaa actcgatggc ctgttcgtag atcgcctcat cggtcaggat
      301 cgtgcgcact acttctacga gcttcatgag cggcactgga ttaaagaagt gtagaccgac
      361 aaaacgccgc tgccgcccgg ccgatgtcgc agtcatcatt tctgtgatcg agatcgacga
      421 agtgttggag gcgaagatcg tttccggttt gcaaatctga tcgagttgct tatacgtgga
      481 acgtttctct tcgatgtttt caataatcgc ttcgatgatg atgtcacagt ccgccaggtc
      541 gtcgaatgtt gtagtaccag tcagccgtcc gcgaatctcc ttctgctgat cggcggtaat
      601 cgtgcccttt tcagcaaact ttgcgagcga cttctcaata ccaacaaagc ctttcttgat
      661 gagctcatcg gaaacttcgc gaaccaccgt cggaaatccg gcgctggcgg ccacctgcgc
      721 gatgcccgac cccatcaggc cacagccgag cacaccaacc ttctttattt ccatgcctct
      781 ttctcctctt tcactaagca atcttaagaa ccagaacttg aaatgctatt gagacgtttc
      841 gggcttaaca tcggtcaagt cgctgtattt cttgcccaca gtccgcaacc actcattgta
      901 gtctttggcc cagacgccaa acctttgaat tagctgcgac ttttcctgct tcttcgcatc
      961 agcgttagtc gttgttccct ccgtcgcatt aacggttgcc gcttccaata acagatttct
     1021 catcgtttcg cgcagctcgt gtgctttctg atccaagtag ggcgcattgt tcagattttg
     1081 cactgcgctc gcgatgggct gtgagtcgcc ttcctgaacc gccaaataga atgcctcctg
     1141 agcttcgttc atcgcctttg cataaacaac aaagctatcg gcggtggttt tgtattggat
     1201 gggagggaga actattgaag ctgttgccgg atcgcgaaag tgtctggcga cctggacgaa
     1261 gctaattgcc accagcgcca gcgccagaac tcttaatatc tgccagacga ccgccacgcc
     1321 cgatctttcg cccgggcgcc ggtagtcgac caccaaagcc aacgccgcgc cggcagtgag
     1381 cccgcccagg tgagccgcat tatcgataat gccgcgtccg acatagccga tgaaaagatt
     1441 gagcacgatc atcggcaaca agccgatgcc gaaggcccgc ttaaaacctt ccggtaactc
     1501 gtgccggaac ttgatgccaa agacaaagag cacacccacc agtccaaaca aggcgcccga
     1561 cgcgcccgct gatggttcgt catgaatctt aaataggaat cgtcccagcg caccgagcgg
     1621 agcatcggga cttacaaccg tgaggtaact ggcgaccacg ccggcaatcc ccgtagccac
     1681 ccagagaacg acaaacttcg aagagccgta aagcttctca acgtatggcc cgatgatcca
     1741 gagactgtac atgttaacca gcaagtgcag cagattgaca tgcacgaaca ttggcgagac
     1801 gaaccgccac cactggtgac cggttgtgat taatgagttg agtttagccc cgtatgcgat
     1861 caggacgtga aaaggaaaca cgccgagcat gctgaactgc atgccgctgg actgccacat
     1921 caatagatag ataaagaaat tcgtcaccag cagaatgatg gtgaacttgt aggggcgctc
     1981 catcagcgcg cgggcaaact tcacggtttc gcgatcggga acggtgcgag taggacgagg
     2041 gctggctgga gcagaagtcg aagcgccgca aaccgcgcag gtagtttgtc cggcgcccac
     2101 gatagcacca caactgcgac acatcgtggg ccgtggtgga gtcgggttga attggctagc
     2161 taacaagttt ctaagataac aagcgtgatg cttggactac aaagcatcac gctcaaggtc
     2221 ccgcttgatt ggaaaagcat tagtttgcgg ggcgtgtcca gcaatcacga gctggttatt
     2281 atgccgtgct ggctttcgac gctcctattt cctgaagcag gcttacgaga tcgtcggcgt
     2341 gttcttcttc catcgcaagt atcccttcca gcattctgcg cgtggtcgtg tcgtcgttgc
     2401 caacgtaatt gatcatctcg cgataactgt cgatagcaat gcgttctgcg actagatcct
     2461 ctttaatcat gtcaacgagg cccggactgt cgtcgtattc agcatggctg cgcgtcagca
     2521 gaccttcagg gtcgaaattc ggctcgcccc ctaattgcac tattcgttgc gcaatcaagt
     2581 cgccatggcc ttgttcttca ttcgcatgct gaagaaactc ggctgcgact ccctcggcat
     2641 taattcccga agcggtaaag taatgccgtc ggtaacgcag cacgcaaaca atctcggttg
     2701 cgagagcttc attcaacagt ttgattacgg tcggtaaatc cgcggtatat cccggcgtaa
     2761 ttgcaccttt ctcgatgtgt tggcgggcgc gttcgcgcag agtcttgata tcagtcagaa
     2821 atggtttatc agccacgatg ttgctcctga agattaagtt gttaggggag ttcgatatta
     2881 ctcgctggtg gaaacctgag caagctagga ggaaaagtgg gccccttcaa cggtagggcg
     2941 gtcgaactat tccgctgtga ctcggccttg aattcttatc tctgttgagg cggtgggatc
     3001 tttcaccacc gagataggcg cacgccagac gagcgtcttg atccggcacg tgccggtatt
     3061 gtcttcgcgg cagtagaaga gtgtcacctg tatccttagt tcagcatcac cacccgctga
     3121 cgacgcgaag ggaacgagta tcggtagctt tagactgttc gacgtctgct ttgtctcttt
//
LOCUS       DASUEX010000621         6181 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1700295, whole genome shotgun sequence.
ACCESSION   DASUEX010000621 DASUEX010000000
VERSION     DASUEX010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6181
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1700295"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(98..256)
                     /locus_tag="VFV61_10335"
     CDS             complement(98..256)
                     /locus_tag="VFV61_10335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900920.1"
                     /translation="MANDGGGGGSAGVVAIVAIFVIIILVGLFMFRGRLFGGGTQKID
                     VNVQTPSR"
     gene            complement(464..1501)
                     /locus_tag="VFV61_10340"
     CDS             complement(464..1501)
                     /locus_tag="VFV61_10340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900921.1"
                     /translation="MNFSLAPNKWLTSGKFAFAALLALTFCIAYPGSAAYAKKRKPAK
                     YGIIKIQTSPAGLPIEIDGKPDGTTTIDWRSWERTPGVHTVVISLPDGQRWTREISLD
                     PGRIKCVALNYRPGVPPTVSPCPYPVNLSAPVSVNEGDVITYTADVTYSGSSALNYTW
                     TVSPATAKILSGLGTPTISVDSTGLGNQTVSATLVVNDGSGEAACRQVAHAATNIVRI
                     PPPPREGREFDVCCSCSFDDQKARLDNLTVELQNDPSATAYIFGYGGRTSRPGEADRL
                     VTRARDYLVNERAIDPARIIVVNGGYREEDCVEMWIVPSGAAAPQPRPTVQPSDVRPA
                     AEAPARKRRRG"
     gene            complement(1507..2082)
                     /locus_tag="VFV61_10345"
     CDS             complement(1507..2082)
                     /locus_tag="VFV61_10345"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEX4900922.1"
                     /translation="MANTPAYAGPVAIGDVLQIFSSYQNPPALRLPNVSRTYGGAVVG
                     STASSPGVDTVEYSSTSRTPESLITGVTLNQDPQKTVTIVAQGDVDATVCDCGEVTVA
                     AGFPKWPLLFLAAIPLFFIHDKDCDDCDEIIPNPTPTPTPTPTPTPQTPVPEPASLLL
                     FGSGLAAVGASLRRRRMKAKLKTQGEITEEG"
     gene            complement(2362..4500)
                     /gene="pnp"
                     /locus_tag="VFV61_10350"
     CDS             complement(2362..4500)
                     /gene="pnp"
                     /locus_tag="VFV61_10350"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009567342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEX4900923.1"
                     /translation="MPVKKYLKESIKLGDKELTVETGRIAKQADGSVVIRYGDTMLLV
                     AAVSATTPREGVDFFPLTVEYRESQFAAGRIPGNYFRREGRPNEKEVLTCRLIDRPCR
                     PLFAEGYRNETQVIATVISADTENNPDVIAITGASCALFLSDIPFMNPIAGVRVGLID
                     GRYIINPTYDEVRESRLNLVVAGTEEAIVMVEAGASEVSEEIMVEALMLAHKEINRLC
                     RWQNELYKALSIQKREVTPPILNEEMLEEIKRNYSVRLRDALDTTNKEKRDSYAAVDA
                     LKKEVVDAYPADLLEQRQMAKAIFDHLKESIFREDILDKRRRPDGRRFSEIRPIHCEV
                     GWLPRVHGSALFTRGETQAMVTATLGTKEDEQYMDDLEKGELKRRFLLHYNFPHYSVG
                     EVGRFGGSSRREIGHGALARRAIEAVLPDDADFPYTIRIVSDITESNGSSSMASVCGG
                     VLSLMDAGVPLKAPVAGVAMGLVMEGNKYAILSDIAGAEDHYGDMDFKVTGTRDGITA
                     LQMDIKIGGINAQIMAEALEQAKKGRMHILNVMEQAIAAPRADISAYAPRIIQIKINP
                     DKIRDVIGPGGKIIRSLVEETGAKIDVSDDGTISIATASGAAAEAAVARIRGLTAEAE
                     IGQSYLGTVSRIVDFGAFVEIFPGTDGLLHISEIADRRVKDVRDELKEGQQILVKCIG
                     KEGNKIKLSRKAVLRDEERKAEAASAGESD"
     gene            complement(4817..5086)
                     /gene="rpsO"
                     /locus_tag="VFV61_10355"
     CDS             complement(4817..5086)
                     /gene="rpsO"
                     /locus_tag="VFV61_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEX4900924.1"
                     /translation="MSLAKETKAQILTDFRTHEADTGSPQVQVALLSKRINELTEHFK
                     THKKDNHSRRGLLKMVSQRRSMLDYLKRTDIERYHEVVSRLGLRR"
     gene            5358..>6181
                     /locus_tag="VFV61_10360"
     CDS             5358..>6181
                     /locus_tag="VFV61_10360"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX4900925.1"
                     /translation="MTVPQTRVVIIGGGIGGLFTALELAGEAQITLISDEDHFLFTPM
                     LYEYLSGEVEDWHIAPKYKEVLDERIEFIQGEVTEIDLQKQQITVDKHSELLSYDILV
                     LGVGGVTNYAGVEGAQQFAIPFRKIAHADLLRQRMVDALDHVPPNLPPQDTRRALTFA
                     VVGAGASGVELSTKMADLLRDAFERRALTGEPRVLVIEMGDKVVPGMGDQIREFVTEA
                     LAESRVEVHTLTRVVRLKERSLTFSHGGIETEIETAAVVWAGGVQVNPLVERLEIP"
BASE COUNT         1378 a         1692 c         1689 g         1422 t
ORIGIN      
        1 gaactcaaat tagtaaacgg gagcttcgga tcgcgaacaa gaaacgagga gcgcagatca
       61 cgctcctcgt cttttgaaaa accgaatagg ttactggtta tctactgggc gtttgcacgt
      121 taacgtcaat tttctgagtg ccgccgccga acaatcgtcc gcggaacatg aacagtccca
      181 ctagaattat tatcacgaag atggcgacaa tcgccacgac gccggcgctc ccgccacctc
      241 ctccatcatt agccatgaag gcatcctcct gttaaatgag cctgaagaaa gggggcgttt
      301 aaatcggggg aggtgcggct ctgtagttac cacaaggggg gagaagtctc aagtgccaag
      361 tctcgggtct caaatctcag gtcgcgggac gtcacgcaaa agtctcaagt cccgaaactc
      421 gagacttgag acctgtgtgc ttgagaccta agacttaggg gggttaaccg cgtcgccttt
      481 tgcgagccgg ggcttctgct gccggacgaa catcgctcgg ctgcacggtt ggccggggtt
      541 gcggcgctgc ggcgccactg ggcacgatcc acatctcgac gcaatcttct tctcggtaac
      601 cgccattgac tacgatgatc cgggccgggt cgatggcgcg ctcgttaacc agataatctc
      661 gcgcgcgcgt aacgaggcga tcggcttctc ccgggcgact tgtgcgccca ccataaccga
      721 aaatgtatgc ggtcgccgac gggtcgttct gcagctcaac ggttagattg tctaaacgtg
      781 ccttttgatc gtcaaacgaa caactacagc agacgtcaaa ctctcggcct tctcgcggcg
      841 gcgggggaat gcgcacaatg ttggtcgccg catgggcaac ttgccgacac gcagcctcac
      901 cggaaccatc gttcacaacc aaagttgccg atacggtttg atttcctaat ccagttgagt
      961 cgacggaaat tgtgggcgtg cccagcccgc tgaggatttt tgcggtagcg ggacttactg
     1021 tccaggtgta attcagcgct gaacttccac tgtaagtaac atcggccgtg taggtgatca
     1081 cgtcaccctc gttaactgat acaggggcag agagattaac cgggtaaggg caaggagaaa
     1141 cagttggcgg tactcctggt cggtaattga gcgcgacaca tttgatacgg cccggatcga
     1201 gactaatctc gcgggtccaa cgttgaccgt ctggaaggct aatgaccaca gtatgcacac
     1261 ccggagtgcg ctcccaggag cgccaatcga ttgttgtcgt tccatccggt ttgccgtcaa
     1321 tctcgatcgg caatccggcg ggagaagttt gaatcttgat gattccatac ttcgccggct
     1381 ttcgcttttt tgcgtaagcg gcggaaccgg gataggcgat acaaaaggtg agggcgagca
     1441 aagcagcaaa cgcaaacttg ccgcttgtga gccatttgtt cggggctaaa ctaaaattca
     1501 tcttggttag ccctcctccg tgatttcgcc ttgcgtcttt agcttcgctt tcatccttcg
     1561 ccggcgcaga ctcgcgccaa cggcggccag tcctgatccg aacaatagta gcgaggcagg
     1621 ctctggaacc ggggtctgag gagtgggcgt tggcgtcggc gtcggcgttg gcgtcggatt
     1681 cgggattatc tcgtcgcagt cgtcgcagtc tttatcatga atgaagaaca atggtatggc
     1741 cgctagaaag agcagcggcc acttggggaa cccagcagct acggtgactt caccgcaatc
     1801 acaaacagtc gcatccacgt caccctgtgc aactatcgtt acagtttttt gaggatcctg
     1861 attcaaggtg acccccgtaa tcaacgattc gggggtacgt gaggtcgacg aatattcaac
     1921 agtgtcaaca ccggggctgg atgcagttga acctacaacg gcgccgccgt aagtccggga
     1981 aacgttaggc agacggagcg ccggtggatt ttgatagctg ctgaatatct gcaacacatc
     2041 gccaatggcc actgggccgg cataggccgg ggtgtttgcc aaaagcaata ttgctagtag
     2101 tacggctacg gggcgaagcg ctcgactcct cattgattag aatatccttg tgggtctttc
     2161 ccactgttgt tgggaaaaga tttcatcctt ataccctgac tgaagcccga gtgggtgaaa
     2221 gctcggctta gctggtgtta agaggagatg ttcaatgaga tggtgattcc attataggac
     2281 caaaattggc cggcggtaaa caagcaaaag ggaggtctgc tggtggcaaa cctcccaatg
     2341 gatgtaaaac gacgttggca cttaatctga ttcgccagca ctcgcggctt cagccttgcg
     2401 ctcctcgtcg cggagcacgg ctttgcgaga gagtttgatc ttgtttccct ctttcccgat
     2461 gcacttcacg agaatttgct gaccttcttt cagctcgtcg cgtacgtctt ttacacgacg
     2521 gtctgcaatc tccgagatgt gcaacaagcc gtcagttcca gggaaaatct ctacaaacgc
     2581 cccaaaatca acaatcctcg aaactgtgcc caggtagctc tggccgattt ccgcctcggc
     2641 tgtaagaccc cgaatccggg ccacagccgc ctcagcagcg gccccactgg cggtggcaat
     2701 cgaaatagtc ccatcgtcgg aaacatcaat ctttgcccca gtctcttcga ctaaagagcg
     2761 gatgatcttg ccgcctggtc cgatgacgtc gcgaattttg tctggattga tcttgatttg
     2821 gattatccgc ggcgcgtagg ccgagatatc agcacggggc gcggcaatcg cctgttccat
     2881 tacatttaga atgtgcatgc gacccttttt cgcctgctcc agcgcctcag ccatgatctg
     2941 cgcattgatg ccaccgatct tgatgtccat ctgcaacgcc gtgatgccgt cacgcgtacc
     3001 agtgactttg aaatccatgt cgccgtagtg gtcttcagct ccggcaatat ccgacaggat
     3061 ggcgtattta tttccttcca tcactagtcc catcgccaca ccggccactg gtgccttgag
     3121 tgggacgccg gcgtccatca gcgacagcac gcctccacag acgctggcca ttgaagaaga
     3181 accgtttgac tcagtgatat ccgaaacaat gcgaatggtg taaggaaagt ctgcatcatc
     3241 cggaagcacg gcttcgatcg cgcgccgcgc taaggcacca tgtccgatct cgcgccggct
     3301 ggagccccca aagcgaccaa cttcgccgac tgagtaatgc gggaaattgt agtggagcag
     3361 aaagcgacgt ttgagttccc ctttctcgag gtcgtccatg tactgctcat cttctttcgt
     3421 gcccagcgtg gcggtgacca ttgcctgagt ttcaccgcgc gtaaaaagcg cggaaccgtg
     3481 tacccgcggc aaccaaccca cctcgcaatg gatggggcga atttctgaga agcggcggcc
     3541 atccggacgg cggcgtttgt caagaatgtc ttcgcggaaa atcgattcct tcaaatgatc
     3601 gaaaatcgct ttcgccatct gacgctgctc cagtaaatcg gccggatacg cgtcgaccac
     3661 ttccttcttt agggcgtcaa ccgccgcgta gctgtcgcgc ttttccttgt tcgtagtatc
     3721 aagagcatcg cggagtctta cgctgtagtt gcgcttgatc tcttccaaca tctcttcatt
     3781 aagaatcggg ggtgtaactt cacgcttctg gatcgagagc gctttgtaga gctcgttctg
     3841 ccagcgacaa agacgattga tttctttgtg cgccaacatc aacgcctcaa ccatgatctc
     3901 ttcagagacc tcggaagcgc ccgcttccac catgactatg gcttcttcgg tgcctgccac
     3961 caccaggtta agacgcgact cgcgcacttc gtcgtaggtg gggttgataa tgtaacgccc
     4021 gtcaatgagc ccaacgcgga caccggcgat cgggttcatg aagggaatgt ccgaaaggaa
     4081 caaagcacac gatgcgccgg tgatggcgat tacatccgga ttgttttccg tgtcggccga
     4141 gatgacggtg gcgattacct gagtttcatt tcgataacct tcggcgaaca acgggcggca
     4201 gggtcgatcg atcaaacggc aggtgagtac ttctttctcg ttgggacgac cctcgcggcg
     4261 gaagtaattg ccgggaatcc ggccggcggc aaactgtgat tcacggtact cgaccgtgag
     4321 cggaaagaaa tcgacaccct ccctgggtgt ggtcgcactg actgcggcaa ccaacagcat
     4381 cgtgtcgccg taacggatga cgacggagcc atcagcctgt ttggcaatgc ggccggtctc
     4441 gacagtcaac tccttatcac caagtttgat cgattcttta aggtacttct taactggcat
     4501 gtctttgtct ccttcgggac gataagcgcc tgaatatcag gcccgcgggc gtctcatccg
     4561 cctcgcgttt gtaaggttaa aacttcgcct ggcttcccgg ttaacgtgat ccggcaactc
     4621 aagctgcaat aacagtaaag gcggcacgag cctaaccgtg aagctcatgc cgcctttgat
     4681 ggaaaactcg taggctaacc acgtctactt gctgggagtc tcgccgtcac gttcggcacc
     4741 ctcgcaagtt cttcgatcga cgtagagtct aaatccgaga ttcgaagatc tctcgtgctg
     4801 taaaactaaa cttgtcttaa cgacgcaggc ccagacgact cactacctcg tggtaacgtt
     4861 caatatctgt gcgtttcaaa taatcaagca tgctgcggcg ctgtgagacc atcttaagca
     4921 ggccgcgccg cgaatgatta tcttttttgt gggtcttgaa atgctccgtg agttcgttga
     4981 ttcgcttgct cagcaaagct acttgaacct ggggtgagcc ggtgtcggcc tcgtgggtcc
     5041 ggaaatcggt caaaatctgt gctttcgtct cttttgctaa cgacactgcg taaaatgatc
     5101 ctcctcggca gcagtaagtc cgcctaaaga cggcaaccgc taaacttaaa aagcggttca
     5161 atctaacacg cggcttcgcg aagtgtccag ccgagggtgt tttgcacgtc cagggcatgg
     5221 gatcgggaag gtgctctctt cgggccgcgg gcgtattgcc agcaatcagg gggaagcccg
     5281 aaccctgggt ggtttcgcct gtccccattc catttgctac catcgagccc ccaaaactca
     5341 aaacttaagg attactgatg acggtgccac aaacacgagt tgtgattatt ggcggcggta
     5401 ttggcggttt atttaccgca ctcgaacttg ccggagaagc tcagattacc cttatcagcg
     5461 atgaagacca cttcctcttc acaccgatgc tttacgaata cctcagtggc gaagttgagg
     5521 attggcacat tgctcccaag tacaaagaag tgctcgacga gcggattgaa ttcatccagg
     5581 gtgaagtcac cgagatcgat ttacagaaac agcaaattac agtcgacaag cacagcgagc
     5641 tgctgtcgta tgacattctc gtcctcggag ttggcggcgt caccaattac gcaggagtgg
     5701 aaggcgcgca gcagttcgcg atcccctttc gcaagatcgc gcacgccgac ctgctgcggc
     5761 aacgcatggt ggatgctctc gatcacgtgc ccccaaacct gccgcctcag gacacgcggc
     5821 gcgctctaac atttgcagtc gtcggggccg gcgccagcgg cgtggagctc tcgactaaga
     5881 tggcggatct cctccgcgat gcgtttgagc gccgcgcttt aacgggtgaa ccgcgcgttc
     5941 tagtaatcga gatgggtgac aaagtggtcc cgggaatggg cgaccagatt cgtgagtttg
     6001 taactgaagc tttggccgaa tccagggttg aggtgcatac cctaactcga gtggttcgcc
     6061 ttaaagaaag gagtctgacc ttctcacatg gcggtatcga aactgagatc gaaactgctg
     6121 ctgtggtctg ggccgggggc gtgcaagtca atcctctcgt cgaacggctt gagattccca
     6181 a
//
LOCUS       DASUEX010000622         2876 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_110876, whole genome shotgun sequence.
ACCESSION   DASUEX010000622 DASUEX010000000
VERSION     DASUEX010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2876
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110876"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            130..2643
                     /locus_tag="VFV61_10365"
     CDS             130..2643
                     /locus_tag="VFV61_10365"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX4900926.1"
                     /translation="MDELTTNTTLSHYRIVSKLGAGGMGEVYLAEDSKLDRKVALKIL
                     PADVAGDETRMRRFVQEAKAASALNHPNIITIHEIQQIDSIHFIATEFIDGETLRERI
                     RVGLKLSEVLDAAVQIASALSAAHAAGIVHRDIKPENIMIRHDGIAKVLDFGLAKPTE
                     RLRPESVDTEMATSIKTDPGTVVGTAIYMSPEQAKGTKVDERTDLWSLGAVLYEMMTG
                     RPPFFGETPTETISLILQKEPEPLTRYAREVPAELERIVNKALTKNCDERYQSAKDLL
                     IDLRNLKRKLEVDAEIDRTVSPEFRDAQSARSTGIGQSAPATASVAAATATAGATQTA
                     SSVEYIVSGIKRHKLAALITIVVLALAALALTAYLRSRNAEGSIDSIAVLPFENRSID
                     PETEYLSDGLAESLIYRLSQLPNLRVTPTSVALAYKGKQTDAIKAGNELGVSAVLSGR
                     IIQRGDNLTISAELVDVRYNKLLWGEQYDRKMSDLLATQREIAREIVEKLRLKVSGQE
                     IGIAKHYTESNEAYQLYLRGRFYWNKRTEEGMRKSLEYYQQAIERDPNFALAFSGLAD
                     SYDLLGAADASGGMPPHEAMPKAKAAALRALEIDDTLAEPHVSLAHVKYYYDRDWATA
                     EREYKRAIELNPNYPTAHQWYAVYLMSAGRFDEAVAKNRRAQELDPLSLPINMTLGWV
                     LFNARQYDQSAEQLRKTLEMDPNFALAHHRLGLVYEQQQKYDEAIAEFKQVVNLSPGK
                     PLGIAALAHGYALSGNRAEAQNSLAELRELSKRRYVSPASIALIYAALGDKDRAFVWL
                     EKADSERDANLARLTVDPRFDSLRSDPRFTDLVRRLGLH"
BASE COUNT          713 a          779 c          769 g          615 t
ORIGIN      
        1 cagtgaattg ggcttcccga actggaatcc tgaagtcgcc cgtgagtcac attaaggaac
       61 tgaagtgcag gctggtgccc ggacaagagc tataacattc gttcgacgat gtcaccgaga
      121 gtaatcgcaa tggatgagtt aaccactaac accacccttt cgcactaccg catcgtctcc
      181 aagctcggtg ccggaggtat gggcgaggtg tacctggcgg aagactcaaa actcgatcgc
      241 aaagttgcac tgaagatcct gcctgctgat gtcgcaggtg acgagacgcg gatgcgccgc
      301 tttgttcagg aagcaaaggc tgcttccgct ctcaatcatc caaacatcat cactattcat
      361 gagatccagc agatcgactc gatccacttt atcgccaccg agttcatcga tggcgagacc
      421 ctgcgagagc gcatccgcgt ggggctgaag ctcagcgaag ttctcgatgc cgccgttcag
      481 atcgccagcg ctctttccgc agcgcacgcc gctggcatcg tacatcgcga cattaagccc
      541 gagaacatca tgatccgtca tgatggcata gcgaaggtcc tggactttgg tttagcgaaa
      601 ccaactgaac gactacgacc ggagtcggtt gacaccgaga tggcgactag cattaagaca
      661 gacccgggca ccgtagtggg gacagctatc tacatgtcgc cggaacaagc gaagggcact
      721 aaggtagatg agcggaccga cttgtggagc ctcggtgcag ttctctacga aatgatgacc
      781 ggacgccctc cgttcttcgg cgagactccc actgaaacaa tttctctgat tctccagaaa
      841 gagcctgagc cactgacgcg ctatgcccgt gaagttcccg ccgagctgga gcggatagta
      901 aacaaggctc tcacaaaaaa ctgcgatgaa cggtatcagt cagccaaaga tctgcttatc
      961 gatttgcgca acttgaaacg caagctcgag gttgatgctg aaattgatcg caccgtttcg
     1021 ccggagttcc gcgacgcgca atcagccaga tccactggca ttggtcaaag tgcgccggcc
     1081 accgcctccg tggctgcagc gactgcgaca gccggcgcta ctcaaacagc ttccagcgtt
     1141 gaatacatcg tttccggaat taagcggcac aagttggcgg ctctgattac tatcgtggta
     1201 ttagctctcg ccgccttagc tctcactgca tatctgcgga gccggaatgc agaaggttcg
     1261 attgactcga ttgccgtgtt gccattcgaa aatcgcagca tcgatccgga aaccgagtat
     1321 ctgtccgacg gcctggctga atctctaatt taccggttgt cgcagttgcc caatctgaga
     1381 gtaaccccga ccagtgtagc cttagcctat aagggcaagc agactgacgc cataaaagca
     1441 ggcaatgagc tcggcgtaag tgcagtcctc tcaggaagga tcatacagcg cggcgacaat
     1501 cttacgatca gcgccgaact agtggacgtc cgttacaaca aactgctttg gggtgaacag
     1561 tacgaccgca agatgtcaga cttgctggcg acccagcgag aaattgcccg cgaaattgta
     1621 gagaagttga ggctcaaggt ctcgggccag gaaataggga ttgcgaagca ctacaccgag
     1681 agcaatgaag cttatcagct ttatctcaga gggcggtttt attggaacaa gcgaaccgaa
     1741 gaagggatgc ggaagtcgct cgagtattat cagcaagcga tagaaagaga tcctaatttc
     1801 gcgctggctt tcagcggtct ggctgacagt tacgatcttc tgggggccgc agatgctagc
     1861 ggcggcatgc cccctcacga ggcgatgcca aaagcgaagg ccgctgcgct cagagccttg
     1921 gaaatcgatg acactctggc tgaacctcat gtttcgctcg cccacgtcaa atactattac
     1981 gaccgtgact gggcaactgc tgaacgcgaa tataaacggg ccattgaact gaatcccaat
     2041 tatccgaccg ctcaccaatg gtatgccgtc tatctaatgt ccgcgggacg gttcgacgaa
     2101 gctgtggcaa agaatagacg ggcgcaagaa ctggacccgc tctcgcttcc tatcaacatg
     2161 actttggggt gggtcctctt taatgcacgc caatacgatc agagcgccga gcaattacgc
     2221 aagaccctgg aaatggatcc gaactttgct ctcgcgcatc accggcttgg gctggtctac
     2281 gaacaacaac agaaatatga cgaagccatc gccgagttca agcaagtcgt caatctgtcg
     2341 ccaggcaaac cgctgggaat cgctgctctg gcacatgggt acgcattgtc gggcaaccga
     2401 gcggaagcgc aaaacagttt ggcggagtta cgggaattat cgaagcgccg ttacgtttcg
     2461 cctgcttcga tcgcgctgat ctacgctgcg cttggcgata aggatcgggc tttcgtctgg
     2521 cttgagaagg ctgacagcga gcgcgacgct aatttggcac gactcaccgt ggatcccaga
     2581 ttcgacagtc tgcgctccga tccgcgtttt acagatcttg tgcgccggtt gggtctgcat
     2641 tgatttgatt cgaaggtgac ctgttgctta ctcacagcaa gccgattgca agacttggca
     2701 cctcaacgat ttttacgaac ccgcaccgtc aatagcccaa gtccttccgc ctcggacaat
     2761 acatccgcag atttggatca gtcgtggagt ttaaagctcc gacctccaca aaaatcatta
     2821 atcctcagca aaatgcgcaa ttcctcaatg tttggtgagg ggatcagttt tggaaa
//
LOCUS       DASUEX010000623         2535 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_795257, whole genome shotgun sequence.
ACCESSION   DASUEX010000623 DASUEX010000000
VERSION     DASUEX010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_795257"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="VFV61_10370"
     CDS             complement(<1..209)
                     /locus_tag="VFV61_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006217226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX4900927.1"
                     /translation="MISPKSFLYSVEGGVATITLNRPERLNALTFEVYRELSDTFAAL
                     RSETAVRVVVVTGAGRAFCSGGDVH"
     gene            complement(215..613)
                     /locus_tag="VFV61_10375"
     CDS             complement(215..613)
                     /locus_tag="VFV61_10375"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX4900928.1"
                     /translation="MTCKFINPETLGSPSGFSNGVLAEPGAQLLFIAGQVAWNPEQKI
                     VSDDFVEQFDRALANVITVVQAAGGSAEQIVRLIIYVTNKAEYRSRTREVGERYRARM
                     GKHFPAMVLVEVKGLLEDQAKVEIEGIAAL"
     gene            complement(780..1310)
                     /locus_tag="VFV61_10380"
     CDS             complement(780..1310)
                     /locus_tag="VFV61_10380"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX4900929.1"
                     /translation="MTLNKPRVFTIALLLVLVGSSIGFAQRTDGPVDFSMRSVDGQTV
                     TAESLRGEVVVLAFGASWLPLSRAQLQGIRKLADEYSNRGVVVYFVSTESEDEKSKNF
                     ASDAQLRAFSEKYGLKVTVLRDPEGAISKGMGVDQLPSIIILNKQGNIHGEPIRGLDP
                     NGKLAEQLATRLDKIL"
     gene            complement(1405..2085)
                     /locus_tag="VFV61_10385"
     CDS             complement(1405..2085)
                     /locus_tag="VFV61_10385"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX4900930.1"
                     /translation="MTKTKEQKPKTGDVVAILLAAGRSRRMGAFKPLLPFGKTTVIGS
                     CISNLRSGGVPDVIVVAGHRAGELKASLGGLVSDHFVVNPDLQSEMSASIACGVRAVP
                     ATARAVLVALTDQPAIPPPVIESILKAWRAGAKLIIPEFNERGGHPVLVDLEFRNQLL
                     NLDPQRGLKALFDAHKSQVHRLKVDSSYIARDLDTWDDYVALHEEVFGAPPISLDPDE
                     IRPVEPTN"
     gene            complement(2190..>2535)
                     /locus_tag="VFV61_10390"
     CDS             complement(2190..>2535)
                     /locus_tag="VFV61_10390"
                     /inference="COORDINATES: protein motif:HMM:NF015415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900931.1"
                     /translation="TNGWRASYQKLRRRGAFDFPVLGVAAYVKSEAGSDRIADARIVL
                     GGIAPAPLLIMEAGQALVGNPLDPDRIQEAAEAAYVRARPLDNTDFVMNWRKQMTREY
                     TLRALRQLADTR"
BASE COUNT          546 a          711 c          705 g          573 t
ORIGIN      
        1 tcgtggacat caccgccaga gcaaaacgcc cgccctgctc cggtcacaac cactacccga
       61 acggcagttt cacttcggag tgccgcgaag gtatcgctca actcccgata gacttcgaac
      121 gtcagggcat tgagacgttc cgggcgattc aaagttatcg tcgccacgcc gccttcgact
      181 gagtagagga aactcttggg actgatcatt gtcgtcaaag cgccgcgatg ccttcaatct
      241 caacctttgc ctgatcttcg agcaggcctt ttacttccac cagcaccatg gccggaaagt
      301 gtttgcccat gcgggcgcga tagcgctcac caacctcgcg tgtgcggctg cgatactcgg
      361 ctttgtttgt gacgtagatt attagacgca caatctgttc tgccgaacca cccgctgctt
      421 ggacgactgt aatcacattg gccagtgcgc gatcaaattg ttcgacaaaa tcgtcactga
      481 cgatcttttg ttcggggttc caggcgactt gcccggcgat gaacagcaac tgcgcgccag
      541 gctcagccag cacgccatta gaaaagccgc tgggtgagcc gagcgtttcg gggttgatga
      601 atttgcatgt catgagttcc agcaaggtat tagattccgc cagacggggc aagcgctttg
      661 attgtgaaac ttggccctgt cgccggcagt cagaaccggg agcgaccgcg accgggtaac
      721 aaaaagagac cgcctcgaga ggcggtccct gacctatctg ggtttgttga agaggtggat
      781 tacaggatct tgtcgagtcg cgttgccaac tgctccgcaa gcttgccatt cggatccaga
      841 ccgcggatcg gttcgccgtg aatatttccc tgcttgttaa gaataatgat ggagggtagt
      901 tggtcgacgc ccatgccttt ggaaattgcg ccctcgggat cgcgcagcac ggtgactttc
      961 aagccatact tttcggaaaa cgcgcgcagt tgcgcgtcgg aagcaaaatt tttcgatttc
     1021 tcgtcttctg attcggtgct cacgaaataa accaccacgc cacggttgct gtattcatcc
     1081 gcgagcttcc tgatgccctg caattgcgcg cgtgaaagcg gcaaccacga ggcgccaaaa
     1141 gcgagtacta caacttcgcc acgcaggctt tcggcggtca cagtctgacc gtctacagaa
     1201 cgcatcgaaa agtccactgg cccatcggtt cgttgggcga aaccaatact cgagccaacg
     1261 agtaccagca gcaatgcgat cgtgaaaacc ctgggtttgt taagagtcat tttgtctcct
     1321 tcgtcggaat ccctcgctgc gagtaacttc gcttacctcg cccgggggta gggccgactc
     1381 accttagatg gccaaccgct ggctttagtt ggttggctca acgggccgga tctcatcagg
     1441 gtccaagctg attggcggcg cgccaaacac ttcctcgtgc agagcgacgt aatcatccca
     1501 tgtatccaga tcgcgtgcaa tataagaaga atctactttt agacggtgca cttgactctt
     1561 atgagcgtcg aatagcgctt tcaatccgcg ttgcggatcg aggttcagaa gttggttccg
     1621 aaattccagg tcgaccagta ccgggtgccc gccgcgttcg ttaaactcgg gaatgatcaa
     1681 cttggctcct gcacgccaag ccttgaggat gctctcaatt accgggggtg gaattgcggg
     1741 ttggtcggtc agcgcgacga gcactgctct cgcggttgcg ggaacagcgc ggactccaca
     1801 ggcaatcgaa gcgctcatct cgctttggag atccggattg acgacgaagt gatctgaaac
     1861 gaggccccca aggctggctt tcaattcacc tgcgcggtga cccgccacga cgatgacatc
     1921 tggtacaccg ccgctgcgga ggttgctaat gcaactacca atcaccgtcg ttttgccgaa
     1981 aggaagcagt ggtttgaagg cgcccatgcg ccgcgatcgg ccggctgcaa gcaggatggc
     2041 gactacgtcg ccagtctttg gtttttgttc tttggtcttt gtcatttgga gtgcggtgac
     2101 agtcttgatt cctcggtcct gagtcgcgct cgaacgaccg agtcttaagt tcaaagatca
     2161 aagaccaaaa tacaaagacc aaaacgcgtc tatcttgtat cagctaactg tctcagcgcg
     2221 cgaagcgtgt attcccgcgt catttgcttg cgccagttca ttacaaagtc ggtgttgtcg
     2281 agtgggcggg cgcggacata agcggcttcg gctgcctctt gaatccgatc cgggtcaagt
     2341 gggttaccaa ctaaggcctg ccctgcttcc atgatcagca ggggcgcggg agcgatgccg
     2401 ccgaggacga tacgcgcgtc ggcaatgcga tcgctgccag cttcactttt gacgtaggcc
     2461 gcgacaccaa ggacgggaaa atcaaacgct ccacgccgcc gcagtttctg gtaagaggcg
     2521 cgccagccgt ttgtg
//
LOCUS       DASUEX010000624         2540 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1701379, whole genome shotgun sequence.
ACCESSION   DASUEX010000624 DASUEX010000000
VERSION     DASUEX010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1701379"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..755
                     /gene="rplD"
                     /locus_tag="VFV61_10395"
     CDS             <1..755
                     /gene="rplD"
                     /locus_tag="VFV61_10395"
                     /inference="COORDINATES: protein motif:HMM:NF012782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEX4900932.1"
                     /translation="NNLSLESEMPTVKVRNLKNEEIGEARLSEAVFGAPFNEPLIHAA
                     VRNFMANARQGTSATKTRGDVSGAGRKLWKQKGTGRARIASLRSPLWKGGGNVHGPQP
                     RDWSYNMPRKMRQGALRSALSERLREGNIVLVDEWKLDQPKTKDFVTSLKKLGLDGKT
                     LIVDSFDNENLFLSTRNVQRAKVVNSFGLNIYDLLYHEKLVLSRAAVSELEQLLGNGQ
                     EATSFPEQAAVAGEEAPAKPKRSRKASKEAAE"
     gene            752..1057
                     /locus_tag="VFV61_10400"
     CDS             752..1057
                     /locus_tag="VFV61_10400"
                     /inference="COORDINATES: protein motif:HMM:NF004363.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEX4900933.1"
                     /translation="MKTVWDIIKSPVITEKALVAKEESQDTRQLLTFRVDRRATKPEI
                     KSAVESIFQVKVDDVRTINFMGKMARRGRFEGRKPSWKKAYVTLKHGEKPVDYGEAI"
     gene            1166..1996
                     /gene="rplB"
                     /locus_tag="VFV61_10405"
     CDS             1166..1996
                     /gene="rplB"
                     /locus_tag="VFV61_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEX4900934.1"
                     /translation="MGIKKLTPTSPARRYQTYLTRDELTVGAEPEKSLLEPKKRISGR
                     NNDGRITVRRRGGGHKRFYRIIDFKRDKEGIPGRITQIEYDPNRSAHIALVTYVDGEK
                     RYIIAPSGLEVGKTIMAGPEADILPGNTLPLINIPLGTQIHNIELRPGKGGQMARSAG
                     AFAQLVAKEGNYAQLRMPSGEIRRVPLVCKATIGQVGNIEHENVSYGKAGRSRWKGIR
                     PHVRGVAMNPVDHPHGGGEGKTSGGRNPVTPWGQPTRGYKTRSNKRTQKMILRDRRTK
                     "
     gene            2134..2427
                     /gene="rpsS"
                     /locus_tag="VFV61_10410"
     CDS             2134..2427
                     /gene="rpsS"
                     /locus_tag="VFV61_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009001861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEX4900935.1"
                     /translation="MARSVKKGPFIDKSIVKVVERVRAGGQRPPVIKTWSRRSTISPD
                     MVGLTFGVHNGKRHLPIYVTENMVGHKLGEFAPTRTFKGHSGTKVEKATKVAK"
BASE COUNT          652 a          612 c          747 g          529 t
ORIGIN      
        1 gaaataactt atcactggag tcagagatgc ctacggtaaa ggtgcgcaac ttaaagaatg
       61 aggagatcgg cgaagcccga ctgtctgagg ctgtttttgg cgcgcccttc aacgagccgt
      121 tgattcatgc agcggttaga aatttcatgg ccaacgcacg ccagggaaca tctgcgacca
      181 agactcgcgg cgatgtatct ggtgctgggc gaaaactgtg gaagcagaag ggcaccggtc
      241 gagcacgaat cgcctcgtta cgctcgccgt tgtggaaagg cggcggcaat gtgcatggcc
      301 cacaacctcg cgactggtcc tacaacatgc cccggaaaat gcggcagggt gctttacgct
      361 ctgcgctttc agagcgcctc cgcgagggga acatcgtgct ggttgatgag tggaaattgg
      421 atcagcctaa aacgaaggac tttgtaactt cgctgaagaa gctcggcctc gatggcaaga
      481 cgttgattgt ggactcattc gacaacgaga acctgtttct ttcaacccga aacgttcagc
      541 gagccaaggt ggtgaacagt tttggtttga acatttatga cctgctttat cacgaaaaac
      601 tggtgctgtc tcgagcagcc gtgtccgagt tggagcagtt gcttggaaac gggcaggagg
      661 ccacttcctt tcctgagcag gcagcggtag ccggcgaaga agcgccggca aagccaaaac
      721 ggtcgcgtaa ggccagcaag gaggcggcag aatgaaaaca gtctgggaca tcatcaagtc
      781 gccagtgatt acggagaagg cacttgttgc gaaggaagaa tcgcaggaca cgcgacagtt
      841 actgaccttc agggttgatc gacgagcgac taagcccgaa atcaaatcgg cagtcgaaag
      901 tatctttcaa gtgaaggtgg acgatgttcg gaccatcaac ttcatgggaa agatggcccg
      961 ccgtggccgt tttgaaggcc gcaagccttc ctggaaaaag gcctacgtaa ccttgaagca
     1021 tggcgagaaa ccggttgatt acggcgaagc gatctgaagc attggcgatg gccaattgcc
     1081 gattggtgat tggcgctgca gagattgagt cgacacggcg gcctgcttaa ttagcaatcg
     1141 ccaatcgaca attggaaata acgagatggg catcaagaaa cttacaccaa catccccggc
     1201 gcgcaggtat caaacctact tgacgcgaga tgagttgacc gtcggcgccg aacccgaaaa
     1261 gtcgctgctc gagccaaaga agcgaatctc cggccgtaac aacgatggcc gtatcactgt
     1321 tcgtcggcgc ggcggtggtc acaaacggtt ctaccggatc atcgacttca aaagggataa
     1381 ggaaggcatt cccggccgca ttacgcagat tgagtatgac ccgaaccgtt cagctcatat
     1441 tgcgctagtt acgtatgttg acggcgagaa gcgttacatt atcgctccca gtggtctcga
     1501 agttggaaag accatcatgg caggaccaga agccgacatt cttccaggta acactctgcc
     1561 gctgattaat attccgctcg gtacgcagat tcacaacatt gagttgcgtc ccggaaaagg
     1621 tggtcagatg gcacgttcgg caggtgcctt tgcacaattg gtggcgaagg aaggcaatta
     1681 tgcccaactc cgcatgccgt ctggtgagat tcgtcgcgtt ccactggttt gcaaggccac
     1741 catcggccag gttggcaata tcgagcacga aaatgtttcg tacggaaaag ctggacgttc
     1801 gcgctggaag ggtattcgcc cgcacgttcg cggcgtggct atgaacccgg tggaccaccc
     1861 gcatggcggt ggcgaaggta agacttcagg cggtcggaat ccggtcacac cctgggggca
     1921 gccgacgcgc ggttacaaga ccaggagtaa taagcgaacg cagaagatga tcctgcgcga
     1981 cagaagaacc aaatgatgct gatcgccaat tggcaattgc cgattggtgc gtgattttcg
     2041 aatgttttgt ttaggaccgg aatgcgagtc acttacgaat agttcaaatt gccaattggc
     2101 aatcgtcaat cgtcaataag cagcggagta tctatggcac gttcagttaa aaaaggtccc
     2161 ttcatcgata agagcattgt aaaggtggta gagcgcgtcc gcgctggtgg acagcgacca
     2221 ccggtgatca agacctggtc gcgtcgctcc acgatttcgc cggacatggt ggggctgact
     2281 tttggcgttc acaatggcaa acgccatttg ccgatctatg tgacggaaaa tatggtgggc
     2341 cacaaacttg gtgagtttgc gccaaccaga acgttcaaag gccattcagg aaccaaggtc
     2401 gaaaaggcta cgaaggtggc gaagtaatta aaggcctttc agcgattaac gatttacgag
     2461 gttgagtatg gaagcgagag caagtgcacg gtatctccga gggtcggcgc agaaggcgcg
     2521 gctggttgtg gatatgattc
//
LOCUS       DASUEX010000625         2597 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_664396, whole genome shotgun sequence.
ACCESSION   DASUEX010000625 DASUEX010000000
VERSION     DASUEX010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_664396"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /locus_tag="VFV61_10415"
     CDS             complement(<1..536)
                     /locus_tag="VFV61_10415"
                     /inference="COORDINATES: protein motif:HMM:NF012320.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEX4900936.1"
                     /translation="MAPDPRHGTGINIFIDDDPPITGARIKVIGIGGGGGNAVNRMIE
                     AGIEGIEFLVANTDLQALKRSRAPIKIQLGSRLTKGLGAGADPGVGREAALEDTDKII
                     EVLEGADMVFVTTGLGGGTGTGGAPIIASLATELNALTVAVVTKPFHFEGRRRMHQAE
                     QGLRELRECVDTVITIPN"
     gene            complement(670..1932)
                     /gene="ftsA"
                     /locus_tag="VFV61_10420"
     CDS             complement(670..1932)
                     /gene="ftsA"
                     /locus_tag="VFV61_10420"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEX4900937.1"
                     /translation="MAKRTQHTIGLDIGTSRVSCIAGETGESGLLNIVGIGEAEARGL
                     RKGVIVNPDAAVDAIKKAVEEAERMSGLQAEEVTINLAGSHILGFNGQAIVAVSGRDR
                     EITTEDVRRAIDSACAIQLPAGREIVDRLPQEFIVDDQDGINDPVGMIGARLAVKVHI
                     VTSPVTARQNAINAVNRAGLIVADMVLEQLAAAEASLTDDDKEFGAALVDIGAETTGL
                     VIYQRGAVQHTAVFPLGGSHFTNDIAFGLRTPIPEAERIKRAVGCACSTSLSEMERGE
                     LVDVPSVGGRAPRQLSRQILCDILQPRAEEVLMHVADEIKDAGWERQLSSGVVLTGGG
                     ALLNGMTEIAEQVFDAPVRIGFPERDRFGGLVEDVQNPAWAAAAGLALVAQRAQASEL
                     RSGTARRGSTGKLTHFVSRFRNRFGSIF"
     gene            complement(1980..>2597)
                     /locus_tag="VFV61_10425"
     CDS             complement(1980..>2597)
                     /locus_tag="VFV61_10425"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEX4900938.1"
                     /translation="DSIRVRIVEREPRVVVRTNAGRLRWVDEDAVFLGEMSTADQMPA
                     FFLRGLSEDDSETAQVENVERVRRFIQFERECVATGIAERVSEVNLSDVRDVRVQLSG
                     EDSQIEVRLGSEEAGKRLKSGLEVLDGQRRTPRGQYISYVDLSQGKRAVIGFVSGAHV
                     ATAAGSATDNVPLNRSAASQRSNQASGSDEQKKKEKKEKNKREAG"
BASE COUNT          536 a          828 c          635 g          598 t
ORIGIN      
        1 tcgttgggaa tggtgatcac ggtgtcaaca cattcgcgta gttcgcgcaa gccttgttcc
       61 gcctgatgca tgcggcgtcg accttcaaaa tgaaacggtt tcgtcacgac ggccacggtc
      121 agcgcattca actccgtggc taaggacgca atgatcggcg cgccaccagt gccggtaccg
      181 cccccgagac cggtagttac aaacaccatg tcagcgccct cgagcacctc aatgatcttg
      241 tcggtgtctt ccagggcggc ttcacggccg acgccggggt cagcgcctgc accaagtccc
      301 ttggttaagc gactgccgag ttgaatctta attggggcgc gagaacgctt gagcgcctgc
      361 aggtcagtat tggcgacgag gaattcgatt ccttcaatac cggcctcaat catgcgatta
      421 acggcattgc cgccaccacc gccgatgccg attactttta tgcgcgcgcc cgtgatgggc
      481 ggatcatcat caatgaaaat attaatgcct gtgccgtgac gagggtccgg tgccatagtt
      541 atgtcctctc taccgaagcg tgaaggcgtc caaggcctct ttctcctatg ttgggtggct
      601 cgtgtcatcg gcctccatat gttgcggaca atatattgtg caaccgggcc gagagcatac
      661 aacaacttgt caaaaaatac taccgaaacg atttctaaat cgtgaaacaa aatgcgtcag
      721 cttgcctgtc gatccacgac gagctgtgcc ggagcgaagt tcgctggcct gcgcgcgttg
      781 tgccactaat gccaagcctg cagcagcggc ccatgccggg ttttgtacat cttcaaccag
      841 tccaccaaat cgatctcgct caggaaatcc gatgcgcacg ggagcatcaa aaacctgctc
      901 cgcaatttca gtcatgccat tcagcaacgc gccaccgccg gtaagaacga ccccactcga
      961 aagttgcctt tcccaaccag cgtccttgat ctcatcagcc acgtgcatca atacctcctc
     1021 cgcgcgcggc tgaagaatgt cgcaaagaat ctgtcgagat agttgccgcg gtgcacgtcc
     1081 ccccaccgaa ggaacatcaa ccaactcacc tcgctccatc tcgctcaggg acgtactgca
     1141 agcgcaaccc accgcccgtt tgatcctctc ggcttccgga atcggcgtgc gcagcccgaa
     1201 ggcaatgtca ttggtaaagt gtgagcctcc gagtggaaag accgctgtat gctgaaccgc
     1261 tccccgttgg taaatgacca ggccagttgt ctcggcgcca atgtcgacca gggccgcgcc
     1321 aaattcttta tcgtcatcgg tgagcgaggc ctcagcggca gccagttgct ccaaaaccat
     1381 gtctgccacg attaaaccag cccgatttac tgcatttatt gcgttttgcc gcgctgtaac
     1441 cgggctggtg acaatgtgca ctttcaccgc caaacgcgcg ccaatcatcc ccaccggatc
     1501 gttaatgcca tcctggtcgt caacaatgaa ttcctgaggc aatcgatcta cgatctctcg
     1561 tccagccgga agctgaatgg cgcacgctga atcgatcgcg cggcggacat cctccgtcgt
     1621 aatctcgcga tcacgacctg aaacggcgac tatcgcctgg ccgttgaatc ccaggatgtg
     1681 ggatccagcc aggttgatag ttacctcctc cgcctgcaat ccactcattc gttccgcctc
     1741 ttccactgct ttcttaattg cgtccactgc tgcgtcgggg ttaacgatta ctcctttacg
     1801 aagtccccgg gcttctgctt cgccgatgcc gacaatattc agcagcccgc tttcgcccgt
     1861 ctcgccagcg atgcacgaca cccggctcgt tcctatatcg agcccgattg tgtgctgcgt
     1921 gcgcttggcc atttagctgt gctccttaac taagatcact gcgccccccc tctccgtcct
     1981 caccctgctt cgcgcttgtt cttttccttc ttctccttct tcttctgctc atcggatcct
     2041 gatgcttgat ttgagcgttg cgacgcggcg cttcggttaa gcggaacgtt gtcggtcgca
     2101 ctgccggcag cggttgcgac atgagcgccg gagacaaatc cgatcactgc ccgcttgccc
     2161 tgactgaggt ccacataact gatgtactgc ccgcgcggcg tgcgtcgttg cccatccaga
     2221 acttccagtc cgctctttaa tctcttaccg gcctcctcgg aaccgagacg cacttcgatc
     2281 tgcgaatcct ctcctgacaa ttgcaccctg acatcgcgta cgtcgctaag attgacctcg
     2341 ctaacgcgtt ccgcaatgcc ggtagccaca cactcgcgct cgaattgtat gaatcttcgc
     2401 acccgttcaa cgttctcaac ttgggccgtt tcggaatcgt cttcactcaa tcctctaagg
     2461 aagaaagcgg gcatctgatc agctgtactc atttcgccaa ggaacaccgc atcttcgtcg
     2521 acccaacgaa gacgcccggc attcgtgcga acaaccacgc gtggttctcg ctcgacaatt
     2581 cgtacgcgaa ttgagtc
//
LOCUS       DASUEX010000626         3758 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1366344, whole genome shotgun sequence.
ACCESSION   DASUEX010000626 DASUEX010000000
VERSION     DASUEX010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3758
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1366344"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2746
                     /locus_tag="VFV61_10430"
     CDS             <1..2746
                     /locus_tag="VFV61_10430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900939.1"
                     /translation="QDNNDFGVAGATVGLNNPDLIQEVRLVTNQFTAEFGRNGSAVFN
                     AITKSGSNQFHGSAFWFHNDNALNACSNLDKNAGFCRQPGTPGFRSEKRPFRIENQAG
                     FTFGGPLPLPRFGEGGPSYSSGRDRTFFFFSLQRWWDRQLGNGFTINGVPDAAGQAAL
                     QAAVGNRPQVAALLRHLPASNAACTTACAPLTFTANGQTVSVSRGALTGSTNAALNNW
                     QQSIRIDHNLNKNHTLFGRYIFNDNDQVGSDQATPPGLSTSSLVRSQAVNVSLNSVFS
                     SRVVNELRGAYQRQGQLFTSADPRSEEIPSIEISALGLSGFNAASNRTAIGLAVNLPQ
                     FAFRQTYQLQDNLSLPQGNHGWKFGVDIRRNQLKQFFFPTIRGRLVYSTLQRFVDDVA
                     DTATVNKPLAGGREVFYYDWHDFFAYGQDEWKIRPNFTLTLGLRYESPGQPIGDLVEV
                     NNAIVKTAGGDERFRFTPVPKRDKNNFQPRVGFNWNPRTDSGGILGFLTGGDKLVIRG
                     GYAVTHDYAFTNIASNIASAFPFVAAADLPTTPQPVGPIGVNNAFVTLPTIQGATGLN
                     PNRLTRTIVGDDFRSPYYQQFSLETQRELSANMILRIGYVGTKGTALFQTLDGNPRRI
                     GTATCTETDADTCRVRSTQGIIRHRANTASSIYHSLQVSLEKRLSRGFSAGAHYTWSA
                     FIDTASEIFNPSGAEVAVSQDSFNRSTERARSSFDRPHRLTGNFVYELPLMREQRGMA
                     GRLLGGWQLNSFFTFQSGSPFTVLLGADPAGALNGISGLVGNAIRPNLNTDLNLSGMS
                     IEQIRIAAGGRTCTIAGTAVTGAGCFFSTVSAAQRVGDAGRNILRTDGINRLDFGILK
                     NTRLGENQRIQIRADFFNLTNTRDFGVPEGRANNSNFLNQWGTNGGNRRVIVGIRYVF
                     "
     gene            2901..3548
                     /locus_tag="VFV61_10435"
     CDS             2901..3548
                     /locus_tag="VFV61_10435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900940.1"
                     /translation="MYKLKNSFIVSVGWLVVVSVPSVLSAQDRRPSEASDVQVVKYSW
                     SKERVGWERDPFGGPIENFDEMRVRTRNEKRIMDAKRGGSSVESSKAEREALTDQALI
                     SAIHRAPPARYGFSYRVSFKNNGSKEITAIDWDYVFYDSISETELGRREFGSDQKIAP
                     GKTKELKFFISTPPVRRISVQALEKNERAGLGERVELVRIQYADGTSWQRPQLAP"
BASE COUNT          856 a         1051 c          991 g          860 t
ORIGIN      
        1 acaggacaac aacgacttcg gcgtagcggg tgcgactgtc ggactcaaca acccagacct
       61 gattcaggaa gtgcggttgg tgacgaatca gtttaccgcg gaatttggcc ggaatggatc
      121 cgcagtattc aacgccatta ccaaaagcgg cagcaaccaa tttcatggtt cagcattctg
      181 gttccacaat gataacgccc taaacgcatg cagcaatctc gacaagaacg ctggcttctg
      241 ccggcagccg ggcacaccgg gcttcaggag tgagaagcgg ccattccgca ttgagaacca
      301 ggccggcttc acttttggtg gcccccttcc tttacctcga ttcggtgaag gcgggccctc
      361 ttacagcagt gggcgggatc gcaccttctt cttcttctca ttgcagcgct ggtgggatcg
      421 gcagctgggt aatggcttca ccatcaacgg tgtgccggat gctgccggac aagctgccct
      481 ccaggcagcc gtcggcaacc gcccacaagt agccgccctg ttgaggcatc tgcccgcttc
      541 gaacgccgcg tgcactacag cgtgcgcgcc attgacgttt accgccaatg gccaaacggt
      601 gagtgttagt cgcggcgcat taaccggttc aacgaacgct gccttaaaca actggcagca
      661 gtctattcgc attgaccaca atttaaacaa aaaccacacc ctctttggtc gctacatttt
      721 caacgacaac gaccaggttg gaagtgatca ggccaccccg ccgggtctaa gcacgagctc
      781 cctggtgcgc agtcaggcag tgaacgtttc tctcaacagc gtgttttctt cccgcgttgt
      841 aaatgaactg cggggggctt atcagcgcca agggcaattg ttcaccagcg ccgatccccg
      901 gtctgaggag attccctcaa ttgagattag cgccctcgga ctgtcgggtt tcaacgctgc
      961 ttccaaccgc accgcaatag gtctcgccgt caatctgcct cagtttgctt tccggcagac
     1021 gtatcagctt caggacaatc tctctctacc tcagggcaac catggctgga agtttggcgt
     1081 ggatattcgt cgcaaccaac taaagcagtt cttctttccg actatccgcg gcagattggt
     1141 ttacagcacc ctgcagcgct tcgttgacga tgttgctgat accgctactg tcaacaagcc
     1201 actagctggc ggacgcgagg tcttctacta cgactggcat gacttcttcg cctatggcca
     1261 ggacgagtgg aaaataaggc cgaatttcac actgacgctc ggtcttcgtt acgagtcgcc
     1321 aggccaaccg attggtgatc tggttgaagt gaataatgcg attgtcaaaa ccgccggagg
     1381 tgacgagcgc ttccggttta ctccggttcc caaacgcgac aagaacaact ttcagccgcg
     1441 cgttggcttc aactggaatc cgcgcaccga cagcggcggt atactcgggt tcctcactgg
     1501 tggagacaaa ctcgtaattc gcggcggcta tgcagtcaca cacgactacg cttttaccaa
     1561 catagcttca aacattgcga gcgcgttccc gtttgtcgca gccgccgatt tgccgaccac
     1621 gccacaaccg gtcggcccaa tcggagttaa caatgccttc gttaccttgc cgacgattca
     1681 gggcgctaca ggtctcaacc ccaatcggct gactcgcacc attgtgggcg acgacttccg
     1741 ctcgccttac taccagcaat tcagcctgga gacgcaacgg gaactttctg ccaatatgat
     1801 tcttcggatc ggctatgtcg gcaccaaggg cacggctctc tttcaaactc ttgatggaaa
     1861 tccgcgtcgt atcggcacgg ccacctgcac cgaaactgat gctgacactt gccgtgtcag
     1921 atctacccag ggcatcatcc gccatcgcgc gaatactgcc tcatcgatct accactctct
     1981 gcaggtcagt ctggagaagc gtttgagccg aggattcagc gcgggcgccc actatacctg
     2041 gagcgcgttt attgataccg cgtcagagat cttcaatcct tctggggctg aagtggcggt
     2101 ctcacaagac tctttcaatc gtagcaccga gcgggcgcgt tcgagttttg atcgaccaca
     2161 ccgtcttacc ggtaattttg tttacgaatt gccattgatg cgagaacaaa gaggcatggc
     2221 ggggcgcctg ctcggagggt ggcaactgaa ttcgtttttc acgttccaga gcgggtcgcc
     2281 attcacagtg ctgctgggag ccgacccggc aggtgctttg aatggaatct ccggcctggt
     2341 aggaaacgca atacgaccta acctcaatac ggatctcaac ctcagtggca tgtccatcga
     2401 gcaaatacgc atcgctgcgg gcggacgcac ctgcactatc gccggaactg cggtcacagg
     2461 cgcgggctgc ttcttttcaa cggtgagtgc ggctcagcgc gtcggtgacg ccggacgaaa
     2521 catcctgcgc accgacggaa tcaaccgact tgatttcggc atcttgaaga acacgcgact
     2581 gggcgagaat cagaggatcc agattcgcgc tgatttcttc aacctaacca acactcgcga
     2641 ctttggcgtg cctgaaggtc gcgctaataa ctctaacttt ctcaatcagt ggggcaccaa
     2701 cggaggcaat cgccgcgtga ttgtcgggat ccgttatgtc ttctaacttt ttcctgcgct
     2761 gctgacttta tttgcttagc agcagcgatc gaatttgaac atgggctgga ggttttcccc
     2821 ttcagcccaa tttcttaaag taggtcccgt ttcttttgct tgggtatttt ttctgacgaa
     2881 gaaaaacttc ggggttcgct atgtacaaac tcaagaattc tttcatcgtt tccgtaggct
     2941 ggctggttgt tgtttcagtg ccttcggtcc tcagcgctca agatcgcaga ccctcggagg
     3001 cttcagatgt tcaagtagtg aaatacagtt ggagtaagga gcgggtaggt tgggaacgag
     3061 atccgttcgg agggcctatc gaaaacttcg acgaaatgcg ggttcgcacg cggaatgaaa
     3121 agcgaatcat ggatgccaaa aggggcggca gctccgtgga aagtagtaag gcggaacgcg
     3181 aagcgcttac tgatcaggca ctaatttccg caatacatag ggcaccaccc gctcgctatg
     3241 gcttttccta tagagtttcc ttcaaaaaca acggttccaa agagattact gctatcgact
     3301 gggactatgt gttctatgac tcaatcagcg aaaccgagct gggaaggcga gagtttggga
     3361 gtgatcagaa gatcgctccc ggaaaaacga aagaactgaa gttctttatt tcgactccgc
     3421 cggtccggag gattagtgtg caagcgctgg aaaagaatga gcgcgccgga ttgggagaac
     3481 gggtcgagct ggtacggatt cagtatgccg atggcacctc ctggcagcgc ccacaattgg
     3541 ctccttaacg tagtcactaa tgtagtcatt ggcctaagat cgagctcctt tcaacgactc
     3601 aaccggattg attgcccagt ggggtaggga tatcatgcgc gggcgcgaga ttaactgaag
     3661 cccgaaggtc tgatcccacc cgctcaacgg gaattcaacg gccactacgt ctccagtcag
     3721 acctccagag aatctcatcc tactaaaacc ttacatgc
//
LOCUS       DASUEX010000627         2349 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1366459, whole genome shotgun sequence.
ACCESSION   DASUEX010000627 DASUEX010000000
VERSION     DASUEX010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1366459"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="VFV61_10440"
     CDS             complement(<1..444)
                     /locus_tag="VFV61_10440"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEX4900941.1"
                     /translation="MFIDSHAHIDGPEYDADREDVIQRALEAGVRAILNVGTGDPNKG
                     NLERAVALADAHRGIYAAIGIHPHDARLYTDEVENRIRKLLAEGPRVVAWGEIGLDYH
                     YDNSPREVQREVFRRQLSAAMDCNLPVIIHTREADEDTIEILQQVL"
     gene            complement(625..>2349)
                     /gene="metG"
                     /locus_tag="VFV61_10445"
     CDS             complement(625..>2349)
                     /gene="metG"
                     /locus_tag="VFV61_10445"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF020892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase subunit beta"
                     /protein_id="HEX4900942.1"
                     /translation="EGWFCAACAAYKTEDEYAPAASPGEAPLCLVHETRLDRVSEESY
                     FFRLGDYDEALLSLYESRPDFVLPEARRNEVKSFVRGGLQDLSISRLKTSVSWGIPVP
                     DDPAHTMYVWFDALTNYITAIGFGNEQQDQAVGFQKFWPGLHLVGKDILRFHAVYWPA
                     FLMAAGVEVPRAVVAHGMWLDPAGRKMSKTIGNTIELDVLQRYFTNDAVRYFCLREMV
                     FGQDGRFGYEALIDRTNSDLASGLGNLSSRTLTMISRYADGSVPSGLIADDKLLLAKR
                     TGVDTDETTIAGFVEHARDQFLQQMENFAFSRALEVAWSVVARIDKMITEAKPWDLAK
                     DENQRQTLNAVLYRAAETLRWLSVLLYPVLPEASQSIYDQLGLRDSLGKQDPAKLVWG
                     GLTEGTHIGDIKPIFPRLDKAKVLAEIGKGSAGSGAHATAADAVAASQSKSETRTQPP
                     MKGATEAEAVPGVATLIEIGDFAKVDLRVGEVLTAERLPKSDKLLLMTVDIGEEKPRQ
                     ILAGIAEHYAPEKMLGRKVVIVANLKPRKLRGYESQGMVVAASIGEEGRPVIATFTED
                     VPNGARLK"
BASE COUNT          504 a          690 c          614 g          541 t
ORIGIN      
        1 cagcacctgc tgaaggatct cgattgtgtc ctcgtcggcc tccctcgtat gaatgatcac
       61 cggcaggttg caatccatcg ctgcgctgag ttgtcgccga aatacttcgc gctgcacttc
      121 acgcggcgaa ttgtcatagt ggtagtcgag cccgatttcg ccccaggcga ccacacgggg
      181 gccctccgca agcaactttc gaatcctgtt ctcaacttca tcggtgtaga ggcgagcgtc
      241 gtggggatgt attccaatgg cggcataaat gcctctgtgt gcgtcggcga gagcaacagc
      301 gcgctccagg ttgcccttgt tgggatctcc ggttccgacg ttaagaatcg cccgcactcc
      361 cgcttcaagc gcccgctgaa tcacgtcctc gcgatcggcg tcgtattcgg gcccatcaat
      421 atgcgcatgg gagtcgatga acatgggagg ctagggggaa ggatgaagga tgaaggcgga
      481 cggcggaagt aagcaagaga tcatctcgcc cgcattcgtt aaatgctatt gcaagcgcag
      541 ggtttgccga ctgattgcac gcaacacgaa tgctgttcag ggcgccaacg gcatattcat
      601 tcatccttcc gccttcatcc ttctctattt gagtcgcgcg ccgtttggaa catcttcagt
      661 gaaagtggca atcactggcc gcccttcctc gccgattgac gccgcgacca ccatcccctg
      721 tgactcatag ccacgcaact ttcgcggttt gaggttggct acgatcacga ctttgcgccc
      781 aagcatcttt tcgggcgcat agtgctctgc aataccggcg agaatctgtc gcggcttctc
      841 ttctccaata tccaccgtca tcaggagcaa cttgtctgac tttggcaacc gctcagcagt
      901 caatacctct cccacccgca aatcgacctt ggcaaaatcc ccgatctcaa tcaacgtggc
      961 aacgcccggc accgcctctg cctcagtcgc ccctttcatc ggtggttggg tccttgtttc
     1021 agatttactc tgcgaagcag caacagcgtc ggctgcagta gcatgcgcgc ccgagcctgc
     1081 acttcccttg ccgatctcag ccagcacttt tgccttgtct agtcgtggga agatcggttt
     1141 gatatcgccg atgtgtgttc cttcggtgag tccgccccag acaagtttcg cggggtcttg
     1201 tttcccgagc gagtctcgca aaccaagttg gtcatagatg ctctgactgg cttcaggcag
     1261 cacgggataa agcaacaccg ataaccagcg caaggtctct gcggcgcgat agagcacagc
     1321 attcaatgtc tgccgttgat tttcgtcttt ggccaggtcc cagggtttcg cttcggtgat
     1381 catcttgtca attcgggcta ccaccgacca tgccacttcc agggctcggc taaaggcaaa
     1441 attctccatc tgttgcagga attgatcgcg cgcgtgttcg acaaatcccg caatggtggt
     1501 ttcatctgtg tcaactccgg tgcgtttcgc aagcaacagc ttgtcgtcag cgatcaagcc
     1561 agaagggact gagccatccg catacctgct gatcattgtc agagtccggc tcgaaaggtt
     1621 gcccagtccg ctggcgagat cgctgtttgt gcgatcgatc agcgcctcat aaccgaagcg
     1681 gccatcctga ccaaagacca tttcacgaag acagaagtag cgaaccgcgt cattggtaaa
     1741 gtagcgctgc aacacatcca gctcgatggt gttgccgata gtctttgaca tcttccggcc
     1801 ggctgggtcg agccacattc cgtgagcgac cactgccctc ggtacctcga caccagcagc
     1861 catgagaaac gccggccaat agacagcatg gaaacgcagg atgtcttttc ccaccaggtg
     1921 taacccgggc caaaactttt gaaatccaac cgcttggtcc tgctgttcgt ttccaaaacc
     1981 gattgctgtg atgtagttcg tcagggcgtc aaaccagacg tacatcgtgt gagcaggatc
     2041 atccggaacg ggaattcccc agctaaccga agtcttcagc cggctgatcg agagatcctg
     2101 taagccgccg cgaacaaaac ttttgacctc gttgcggcgt gcttctggca agacaaaatc
     2161 cggtcgagac tcgtagagtg aaagcagcgc ttcgtcataa tcaccaagac gaaagaagta
     2221 gctctcctca gaaacgcgat cgagtcttgt ttcatggacc aggcaaagcg gtgcttcacc
     2281 aggactggct gctggcgcat attcatcttc ggttttgtag gcagcgcagg ctgcgcagaa
     2341 ccaaccttc
//
LOCUS       DASUEX010000628         3018 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_796092, whole genome shotgun sequence.
ACCESSION   DASUEX010000628 DASUEX010000000
VERSION     DASUEX010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3018
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_796092"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..921
                     /locus_tag="VFV61_10450"
     CDS             <1..921
                     /locus_tag="VFV61_10450"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX4900943.1"
                     /translation="AAGIQGPDLMVEFRKQAERFGTEFLEAWITQVDLQERPFTLHTD
                     DYTIKAATLIIASGASAKWLGIPGEAKAPEGLGGNGVSACATCDGPLPSFRDQTLVVV
                     GGGDTALEEATFLTRYASRVFVVHRRDKLRASKIMQDKAFANHKLDFIWDTTVEEILG
                     NQDEGVTAVRLRNLKTDEQHIFPCAGVFVAIGHKPNTDLFKGQIETDEVGYIKTAGHS
                     TATNIPGVFACGDVQDSVYRQAVTAAGTGCMSALDAERFLDHLPVAMPTGEELTIEGE
                     RVSADHSSLITPDGKVTSNKTEVASQETNR"
     gene            complement(918..1373)
                     /locus_tag="VFV61_10455"
     CDS             complement(918..1373)
                     /locus_tag="VFV61_10455"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX4900944.1"
                     /translation="MRRRDLDGCPIAGALQVMGDKWTMLIIRDLAHGPRRTMELHTNL
                     QPISSRTLVGRLRDLVADDILERTDFGGNPPHIEYALTARGRLLIPMLDALRELGLLL
                     NCNECEDRKRRRGAYCETCPKSGWVPVAAEPISRPVRQRRQRDDSIVLL"
     gene            complement(1402..2817)
                     /locus_tag="VFV61_10460"
     CDS             complement(1402..2817)
                     /locus_tag="VFV61_10460"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX4900945.1"
                     /translation="MAKKGAVLIVDDEEIMRDVLETLLSAEGYRVDLAKTGEEGIEAY
                     GKRAYDVVLLDVSMPGIGGLRALEELLKNDAEAVIVMITAYATFDTAIAAWERGAFGC
                     IRKPFQNEQITATVAAGIKRRRKEEERRTLRRAMSRAVDRGEIVGRSDVMQEVFRIVE
                     QVAPARSTVLVTGESGTGKELIAKAIHEASPRASKPFVTVNSSNIPSELLESELFGHT
                     RGAFTGAVAVKKGLFEVADGGSIFLDEIGDIPPETQVRLLRVIQEREFTPLGDTTPRR
                     VDVRIIAATNIDLKEAVKQGSFREDLYYRLAVVPIELPPLRDRREDILALAQHFIVKY
                     NEENGRQVSVHLAPEVLALLEAYSWPGNVRELENTIERAVVIAPGNDITRECLRNEIS
                     DPQSVAARAHDGASMTAVQDIGRGVDFYDEVRRFEIDLIRRALDQTGGHQSRAARLLG
                     MNATTLNSKIKTYNINLRPPG"
BASE COUNT          665 a          870 c          812 g          671 t
ORIGIN      
        1 gcagccggaa ttcagggacc cgatctgatg gtcgagtttc gcaagcaggc ggaacggttt
       61 ggcactgagt ttctcgaagc ctggattacc caagtcgatt tgcaggagcg acctttcacg
      121 cttcacacgg atgattacac aattaaagcc gcaactttga tcatcgcttc cggagcgtca
      181 gcaaagtggc tgggcattcc cggggaggcg aaggctccgg agggattggg gggtaatggt
      241 gtatccgcct gcgccacctg cgacgggcca ctgccttcat ttcgagacca gactctcgtg
      301 gttgtcggtg gcggtgatac ggctttggag gaagcgactt ttctcacgcg ctatgcttcg
      361 cgtgttttcg tcgtgcaccg gcgagataaa ttgcgcgctt ctaaaatcat gcaagacaaa
      421 gctttcgcta accacaaact ggatttcatc tgggatacca ccgtagagga aattctcggc
      481 aaccaggatg agggggttac cgctgtaagg cttcgcaatc taaagactga cgagcagcac
      541 atctttcctt gcgctggcgt atttgttgcg atcgggcaca aacccaacac ggacctcttc
      601 aaaggacaga ttgagacaga cgaggtcggc tatatcaaga cggctggtca ttcaacggca
      661 actaatatcc cgggagtgtt tgcctgtggc gacgtacagg attcggtcta ccggcaggct
      721 gtcacggcgg ctggcaccgg ctgcatgtca gcactcgatg cggaacgatt cctggatcat
      781 cttccggtcg cgatgcctac tggagaagaa ttgactatcg agggtgaacg agtttctgcg
      841 gatcactcgt cgttaatcac gcccgacggc aaggtcactt ccaacaaaac tgaagttgcg
      901 agtcaggaga ctaatcgtta gagcaggact attgaatcat cgcgctggcg gcgctgccga
      961 actggacgtg agattggttc cgcggcgact ggaacccagc cacttttcgg acaggtttcg
     1021 caataagccc cgcgacgtct tttccgatcc tcacattcat tgcaattcag tagcaggcct
     1081 aattctcgta gcgcgtcgag catcgggatc aacagccggc cgcgcgcagt aagggcgtat
     1141 tcgatgtgcg gcggatttcc cccaaagtcc gtacgttcca gaatgtcgtc ggctaccagg
     1201 tctcgtaagc ggccgacgag cgtgcggctg ctaatcggtt gcagattcgt gtggagctcc
     1261 atcgtgcgcc gcggcccgtg cgccaagtcc cggatgatca gcatcgtcca tttatcgccc
     1321 atcacctgta gcgcgccggc aatcggacaa ccatcaagat cacgacgtct cacttgctac
     1381 tccttctcaa aggctccgaa attatcccgg tggccggaga ttaatattgt aagtcttaat
     1441 cttggaattc aaggtagtcg cgttcatccc cagcagccgc gcggcgcgcg attggtggcc
     1501 gccggtttga tctaacgccc ggcgaatcaa atcgatttcg aaacgccgca cctcgtcgta
     1561 gaagtctacg ccacgtccga tgtcctgaac tgcagtcatg ctcgcgccgt cgtgcgctct
     1621 agccgcgaca ctctgcggat cactaatctc atttcgcaga cactcgcgcg taatatcgtt
     1681 tcctggagcg attactaccg ctcgctcgat ggtgttttcc agctcacgaa cattcccggg
     1741 ccaggagtaa gcctcaagca gcgctaatac ctccggagcc aggtgtacag atacctgccg
     1801 tccattctct tcgttgtact tcacgatgaa gtgctgcgcc agggcgagta tatcttcgcg
     1861 ccgatcacgc agcgggggaa gctcaatcgg cacaactgcc agccggtaat aaagatcttc
     1921 gcgaaacgat ccctgcttca ccgcctcctt caaatcaata ttcgtcgccg caatgatacg
     1981 cacatcaacg cggcgcggcg tcgtatcgcc cagcggtgta aactcgcgct cctgaattac
     2041 tcgtaacagg cgcacctgag tttcgggcgg aatatcgccg atctcatcga gaaaaataga
     2101 gccaccatcc gcgacttcaa acaacccttt ctttacggca acggcgccgg tgaaagcgcc
     2161 gcgcgtgtgt ccaaagagct cggactccag gagttcggag ggaatgtttg agctgttgac
     2221 tgtaacaaat ggcttagagg cgcgcggact ggcttcatga atcgccttgg caattaactc
     2281 tttgccggtc ccactttcgc cggtaaccag aactgtggag cgcgcgggag caacctgttc
     2341 cacgatccga aaaacttcct gcattacatc cgagcggccg actatctcac ctcgatctac
     2401 agcacggctc atggcgcgcc gcaacgtccg ccgctcctct tccttccggc gacgcttgat
     2461 gccggccgca acggtggccg taatctgttc gttttgaaac ggcttgcgaa tgcagccgaa
     2521 ggcgccccgt tcccaggcgg caattgcggt gtcaaatgtc gcatatgcag tgatcatgac
     2581 gatcactgct tcagcatcgt tcttgagaag ctcctcgagc gcgcgcagac cgccgatgcc
     2641 gggcatcgag acgtcaagca ggaccacatc gtaggcccgc ttgccatacg cttcgatccc
     2701 ttcctcgccg gtctttgcga gatcaactcg atatccttcg gcggagagca gcgtctccag
     2761 gacatcgcgc atgatctcct catcgtcgac tattaatacc gcgcccttct tagccatgcg
     2821 ttctttgatc tttgagttgc taaaaaaatt gagtttcagt ttaggcgaga acaagacaga
     2881 taacaaaagc cggaggcaaa cagctcaagg ctatctgcgt ccggctcaag aagagtcttc
     2941 accagtgtgc taatcgccta ccgcctgcaa acgggcgcgc aagtcggaag taggcaagct
     3001 gattctgaat gtagtgcc
//
LOCUS       DASUEX010000629         4782 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_325093, whole genome shotgun sequence.
ACCESSION   DASUEX010000629 DASUEX010000000
VERSION     DASUEX010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4782
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325093"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1044
                     /locus_tag="VFV61_10465"
     CDS             <1..1044
                     /locus_tag="VFV61_10465"
                     /inference="COORDINATES: protein motif:HMM:NF020671.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SelB C-terminal domain-containing protein"
                     /protein_id="HEX4900946.1"
                     /translation="FHIHAAEVLARVQVLEAGGQIAPGAEGLAQLRLETPIAAVAGEP
                     FIVRSYSPSLTIAGGKVLDPLASKHRGRDLADVRDRLHVLAGNDPAARLSVFVATSGN
                     LGQRREDLAARTGWENERLAPQLTEAIRERLIVDCGGVLLDHKKYASLREAALTEVRA
                     HHKREPLSRGLGRETLRERHFAHIPSEVFRTILSELEAASLLVSERDLVREKEHVVEL
                     SEPDVALRDRMEALYERAGLEPPGLDEALQATGLTPIQKTHGRKILQLLLDRQSLVRV
                     QGDLYFHSRPLADLTTKLKKFALEHPSDPSLDVAAFKQLAGVSRKYAIPLLEFLDRER
                     ITRRVGDRRQILK"
     gene            1149..1367
                     /locus_tag="VFV61_10470"
     CDS             1149..1367
                     /locus_tag="VFV61_10470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900947.1"
                     /translation="MKSIKDVLAVIVGLAAGAGSIYYFYKFVTFVEAEGGHSYLWKAL
                     GLAVIAFVCGLVYFLGHVNKEEEIHITQ"
     gene            1549..1896
                     /locus_tag="VFV61_10475"
     CDS             1549..1896
                     /locus_tag="VFV61_10475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900948.1"
                     /translation="MIATIALLAQTDTVFREPAYFAPVMLGMLVLGAVAWLIAAVLGF
                     ARAKAFGASARWFSFGAVSLILYHLQFVALGFSLLTKDNSLVFNLLTFFNIFVIIAAL
                     CAIMGFIRLTNPR"
     gene            1916..3709
                     /locus_tag="VFV61_10480"
     CDS             1916..3709
                     /locus_tag="VFV61_10480"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HEX4900949.1"
                     /translation="MTKRLLLIAALAVVVSAAFFFLRGPSREASLRPGKGFVSVKQSR
                     FLIDGEPFRFVGANVAVMYRDEDRAAMPETLRQASQAGMRVVRVWAFGEGGPGDIGPL
                     ADFADWPRTHPFRWAPGQWNEESFVHLDHVLAEAQRNNLRVQLCLTNWWRDTGGVTQY
                     LRWAGINDAADTRFPFGINPERAMLFYTNETTRRLYREHLEKVVTRRNTVTGVFYRDD
                     PTILGWELMNEAQAVTGRWHERRAWFAEMSSYLKSLDANHLITPGAWGYRTAAERREW
                     LADHRLATIDYCDVHNYPREDTNSFVDSPEALREFIDNRAAAALSLSKPLVLGEFGIG
                     PGGYNGISQRDWFRGFFAANLRAGVAGAMFWIFTPDPNRGFGITYTTARDQLVLAEIK
                     NAAVGFAALRAAGPPERLQDPGRHLIPRQFTWSRAAAESPRMILREDRSILYGFKPEQ
                     ASRVRFEKTGSGPGYLWGYGMGSVEYLIPERADRRRVSQIIVRAHVQPVPPVDARPAD
                     IKTRVTLFVNDADCGSRLVSLGPEAEPLIQEWLVDSFSLRLRAMRGLPLTIRFAVTPD
                     ADWPYGINISNWPEGYDNVRDARPVEVEIRR"
     gene            complement(3806..>4782)
                     /locus_tag="VFV61_10485"
     CDS             complement(3806..>4782)
                     /locus_tag="VFV61_10485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900950.1"
                     /translation="KKRMLLFLLVMTCVFLLLNPTILLPATWHEMGIFAGQKRIGHDG
                     YEFMGSLYSHRMTDWLNGTPWYFYLVFIGVKLPLLTVAGFITGLPLLFRRKIGDGRYF
                     IMFWFFVWVMTFMWAGGKFTRYFTVALPAVLITSAIGIQFVGRRLGEKLKGIFSTHGV
                     DVYARTGLVTVVIVLSVVASINATPHFRLYTNMLGGGMDWAGYYFPHDEFYDASMRDA
                     IAEIAQRAGPGTRVASESPILAAYYAERINREDLKCVSLSDAEALSQLQPGDFIIDAR
                     GRRYFSNDDLLSELHRTVAPTFTAALKGVHSADVYQVDDKLLGVLKHK"
BASE COUNT         1028 a         1265 c         1390 g         1099 t
ORIGIN      
        1 ttccatattc atgcggcgga agttctggcg cgggttcagg tgctggaagc tggcggtcaa
       61 attgcacccg gggcggaggg cctagcgcag cttcgcttgg aaacaccaat tgccgcagtc
      121 gcaggtgagc ccttcatcgt gcgctcttac tcgccttcgt taacgatcgc cgggggcaaa
      181 gtgcttgatc cccttgcaag caaacatcgc gggcgcgacc tggcagacgt gcgtgatcgt
      241 ttgcacgttc tggccgggaa cgatcccgcc gctcggttat cggtcttcgt tgcaacttcc
      301 ggtaatctcg gtcaacggag ggaagatttg gcagcgcgca ctggttggga gaacgaacgt
      361 cttgctcccc agctgacaga agcgatcaga gagagactga ttgtcgactg tggcggtgtt
      421 ctgctggacc ataagaagta cgcaagtctt agagaagccg cgctgactga ggtgcgcgcg
      481 catcacaagc gtgaaccgct gtcacgaggc ctcgggcggg agaccttgcg ggaacgtcat
      541 ttcgcccata ttccctccga agtttttcgc actatccttt ccgagcttga agccgcaagt
      601 cttttggtgt cggaaaggga cctggtacgc gagaaggagc acgtagtaga gctttcggaa
      661 cccgacgttg cgctgcgaga tcgaatggag gcactttacg agcgtgccgg cctggagccc
      721 ccaggtctgg atgaagcgct gcaagctacc ggcctgactc cgattcaaaa gactcatggg
      781 cgcaagatct tgcaactgct gctggatcgg cagagtttag tgcgtgtcca gggcgatttg
      841 tacttccata gtcgtccgtt ggctgactta accacgaagc tgaagaagtt tgcgctcgaa
      901 catccgtccg acccctcact tgacgtggcc gcctttaaac aactggcagg cgtctcgcga
      961 aaatatgcga ttccccttct tgagtttttg gatcgcgaac gaattaccag acgagtggga
     1021 gatcggcgtc aaatcctaaa gtaatgggct tgctttaatt tgagggcagg cgttaacatg
     1081 cagcgcatta atagcaacaa cggctttttc caaaaccttc ataacaactc attggccgga
     1141 ggttcacaat gaaaagcatt aaagacgtat tggccgtgat cgttggtttg gcggccgggg
     1201 cgggctcgat ctactacttc tacaaatttg tgacatttgt cgaagccgag ggcggccaca
     1261 gttacctctg gaaggcgctg ggccttgccg ttatcgcttt cgtttgcgga ctggtttact
     1321 tcctcggaca cgtcaacaag gaagaggaaa ttcacatcac tcaataacgg catgcggagg
     1381 atgtctttcc gattgccggt cgcggacccg tccaggccat gagtgtgtga gtgacttctg
     1441 ttacgttgaa ttgcaaacgc cgtttgtgca ctggccgtgg ctagcaggtg cgcaaacggc
     1501 gcttggtatg ttatcgaatc agaaaattta gacggagatg atgccttgat gatcgcaacg
     1561 attgcattgc tcgcgcaaac agatacagtt tttcgcgaac ccgcttattt tgctccggtt
     1621 atgctgggca tgctggtgtt gggagcggtg gcctggctga ttgcggcggt gttgggcttc
     1681 gcgcgagcga aagcttttgg ggcatcggca cgctggtttt ccttcggcgc tgtcagcctg
     1741 attctctacc acttgcagtt cgtggcactg ggttttagct tgttgacgaa agacaacagt
     1801 ctcgttttca atctgctcac gttcttcaat atctttgtga tcatcgcggc actctgcgcc
     1861 atcatgggtt ttattcgact aacgaatccg cgctaaagat ccccacttag ccttgatgac
     1921 aaagagactt ttgctaattg ccgcgctcgc ggtagtcgtg agcgcggcct tcttcttttt
     1981 gcgcggccct tcgcgcgaag cttcgctgcg tcccggcaag ggctttgtca gcgtgaaaca
     2041 gagccgcttc ctgattgacg gtgagccttt ccgttttgtg ggcgcgaatg tggcggtgat
     2101 gtaccgtgat gaagatagag ctgcaatgcc tgagacttta cgacaggcgt cccaggccgg
     2161 aatgcgtgtg gtgcgggttt gggcatttgg cgagggcggc ccgggagaca ttggaccgct
     2221 cgcagacttt gcagactggc cgcggacgca cccgtttcgc tgggcgcctg ggcaatggaa
     2281 cgaggagtcg tttgtccatc tggatcacgt cctggccgag gcgcagcgga ataatttgcg
     2341 cgttcagtta tgtcttacga actggtggcg cgataccgga ggcgtaactc agtatctgcg
     2401 ctgggccgga atcaatgacg ccgccgatac gcgctttcct tttggaatca atcctgagcg
     2461 cgcaatgctt ttctacacaa acgaaactac gcgccggctc taccgcgaac acctggaaaa
     2521 agttgtcaca cgccgcaaca ctgtaaccgg tgttttctat cgtgacgatc ccacgattct
     2581 cggctgggag ttaatgaacg aagcgcaagc ggttacaggc cgctggcatg agcgccgggc
     2641 ctggtttgcc gagatgagta gctacctgaa atctctcgat gcaaatcact tgattacacc
     2701 gggcgcctgg ggctaccgca cggcagccga gcggcgtgaa tggctggccg atcaccggct
     2761 ggcgacaatc gactactgcg atgtgcacaa ctacccacgg gaagatacca acagttttgt
     2821 cgatagtccg gaggcacttc gcgaattcat tgataaccgc gccgccgccg ctttgtcgct
     2881 ttcaaaacca ctggtgttag gagaattcgg gatcgggccc ggtggttata acggcatcag
     2941 tcaaagagat tggtttcggg gtttctttgc tgcgaatctt cgtgccggcg tcgccggcgc
     3001 catgttctgg atcttcacgc cggatccaaa caggggtttt ggtattacct acacgactgc
     3061 gcgtgaccaa ctcgtgctcg ccgaaatcaa gaatgcggca gtgggtttcg cggcgttgcg
     3121 tgcagctggt cccccggaac gactccagga tcccggccgc catctgatac cgcggcagtt
     3181 tacctggtcg cgagcagccg ccgaatcacc acggatgatc ttgcgcgaag accggtcgat
     3241 tctctatggc tttaaacccg aacaggcgag tcgtgtgcgg tttgaaaaga ctggcagcgg
     3301 cccgggttat ctctggggct atggcatggg atcggttgaa tatctgattc cggaacgcgc
     3361 ggaccgccgc cgcgtcagcc aaatcatcgt gcgggctcac gtccagccgg tgccgcccgt
     3421 tgatgctcga ccggccgaca ttaaaacccg cgtgactttg tttgtgaatg acgctgactg
     3481 tggttcacgg ctcgtttctc tggggccgga ggcagagcct ctgattcagg aatggctggt
     3541 tgatagtttc tccttaagac taagagccat gcgcgggctg ccgctgacaa ttcgtttcgc
     3601 agtaaccccg gacgccgatt ggccgtacgg aataaatatt tccaactggc ctgaaggcta
     3661 tgacaacgta agagacgcga ggccggtgga ggttgaaatc aggcgctgat ctcacgcggc
     3721 aaaaatgttc gccacagatg aacgctgatt actgagatcc gaattgtttt gatttctgat
     3781 ctgtgtgttc ggcgtcaatc cgtggctact tgtgtttcag gactcccagt agtttgtcat
     3841 cgacctggta aacatctgcc gagtggactc ctttgagtgc ggcggtgaag gtgggagcca
     3901 cggtccggtg caactcggaa aggaggtcgt cattactgaa ataacgccgc ccgcgcgcat
     3961 caataataaa gtcgccaggc tgcaactgac tgagtgcttc cgcatcggaa agcgagacgc
     4021 actttagatc ttcgcgattg atccgctccg cgtagtaggc ggcaaggatc gggctctcgc
     4081 tggcgacgcg cgttcccggg ccggcacgtt gggcaatttc cgcaatcgca tcgcgcatgc
     4141 tggcgtcata aaactcgtcg tgcgggaagt aatagccggc ccagtccatc ccacctccga
     4201 gcatgtttgt atacaaacga aaatgcgggg tggcattaat tgatgcgacg actgaaagga
     4261 cgatcacgac tgtcactaag cccgtgcgcg cgtaaacgtc gactccatgt gtagagaaga
     4321 tgcccttcaa cttctcgccg agccggcgtc ctacgaattg aatgccaatc gcggaagtga
     4381 tcaagaccgc aggcaatgct accgtgaaat agcgtgtaaa cttgccgcca gcccacatga
     4441 acgtcatcac ccagacaaaa aaccaaaaca taatgaagta gcggccgtcg ccgatcttgc
     4501 gtcgaaacag caatggcaat ccggtgatga agccagcaac cgtcaacaac ggcagtttga
     4561 cgcctatgaa gacaagataa aaataccagg gggttccatt cagccaatcc gtcatgcgat
     4621 ggctgtagag cgaacccata aattcatagc cgtcgtgccc gatccgtttc tgtccggcga
     4681 agattcccat ttcgtgccag gtcgcgggca gcagaatggt ggggttgagc agcaaaaaca
     4741 cgcaggtcat gactaggaga aaaagcagca ttcgcttctt gc
//
LOCUS       DASUEX010000630         4198 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1024149, whole genome shotgun sequence.
ACCESSION   DASUEX010000630 DASUEX010000000
VERSION     DASUEX010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4198
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1024149"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1070
                     /locus_tag="VFV61_10490"
     CDS             <1..1070
                     /locus_tag="VFV61_10490"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX4900951.1"
                     /translation="QTREGGGGHILRINTDRGMITLSQPVAVAAQPVARRDPPRLSGP
                     EASKGAGTPATSAETEEISEGDIIRVDSQLTTLNVSVIDRTTSRGVSGLTRSDFELLE
                     DGTRQEIVQFESSEAPFDLVLLIDVSGSTRDKVKLIRAAALRFVAAARQSDRIAIISF
                     AGQPTLVSPFTLNRELLRQRVEAIDTATGDTKVYDAIEFALRQIPAGTIGSRRTALVL
                     MSDGLDGSIEGVQGDGSQVSFSEVLNHVQEFDGVLYSLWLNTEYEALSPLDTQPEAFD
                     QSHERMKGMAVAGGGAFYEVDRLEDLAGSYERVVADLGTVYSLAYRPANKARDGKWRT
                     IRVNVARPSAVARGKHGYFAN"
     gene            1051..2043
                     /locus_tag="VFV61_10495"
     CDS             1051..2043
                     /locus_tag="VFV61_10495"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX4900952.1"
                     /translation="MATSRTNLKPSGSFLALGVILILALLPALTRAQSTEPNEVIRVD
                     SDLVDLRVSVVRRDPLNPVAELKQSDFLILEDGKPQEISFFAGENDPFDLILLLDLSG
                     STAKKLKLVRSSTERFVEATRPIDRVAIVTFTDVPQVVSPLTLDREALKKSIKKITEP
                     SGGTSFWDSLRFVLDSPATNGMRRRAVVVMTDGVDNALPGIFGDGSRTSFAELLELVR
                     ASDVIVFPVYLDTEKEEIKRHRSPASAYATARENLSQLAEVGGTIVYTARELKDLDRV
                     YDQVIKDLGTVYSLGYRSNNKSRDGKWRSVTVQVIGQPQVSARTKRGYYGRAQN"
     gene            2159..3208
                     /locus_tag="VFV61_10500"
     CDS             2159..3208
                     /locus_tag="VFV61_10500"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HEX4900953.1"
                     /translation="MRKTSTSFPILFCALIVMMAGVATALAQKEGARGQTRDRFVISA
                     RAGGVNAVTGKTGVHAKGSSIWEQLTIKDNLEGGDVVKTGLDGRVEILLNPGSYMRVG
                     ENSEFELTSNSLDNLEVRVIRGTAIVEATGAEDTELLINITTPHAKMAIVRRGLYRVN
                     VVPGDVTEVIVRKGRLLLSDPDAKIKAGKKLIFSGNSYSVAALTDAEKKDVDSFDLWS
                     KDRAHVVAKANQQLTSRDRRILRSGFTDDWAFRFSPQIGGIWFFDSLFGCYSFMPFYA
                     GWGSPYGRSYSRSFLGIPYWQVRNYYWATQRGYFPDASSPVSVPTSIPGKSIGTSPPS
                     GSSFPKGSRGGKVLP"
     gene            complement(3212..3595)
                     /locus_tag="VFV61_10505"
     CDS             complement(3212..3595)
                     /locus_tag="VFV61_10505"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX4900954.1"
                     /translation="MYITIDERDGRPMYRQVVDGIKELIARGELAAGSSLPPVRQVAA
                     DLGVNLNTIAFAYRQLQKEGLIRIRHGSGAVVVSHVTGQTVEDRQHAKVRAALTDLLL
                     AGLSRTEVLALVNKVLNSLVSERGR"
     gene            complement(3979..>4198)
                     /locus_tag="VFV61_10510"
     CDS             complement(3979..>4198)
                     /locus_tag="VFV61_10510"
                     /inference="COORDINATES: protein motif:HMM:NF020317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HEX4900955.1"
                     /translation="KIESAWFAFGDYDVVLIIQMPSNVDAGAIAMAFAAGGACKSVKT
                     TPLLSTAEAVEAMKKASSAGYRAVTAGV"
BASE COUNT          949 a         1165 c         1147 g          937 t
ORIGIN      
        1 tgcaaacacg cgagggtggc ggtgggcata ttctccggat caacaccgat cgcggcatga
       61 tcactctgag tcagccagtg gctgtcgccg cacagccagt cgcacggcgc gatccaccac
      121 gcttatcggg gccggaagcg agcaagggcg ccggcacgcc ggcgactagt gctgagactg
      181 aggaaatcag tgaaggcgac atcattcgtg ttgattcgca actgacaacc ctcaacgtta
      241 gcgtcatcga tcgcaccacg agccgaggcg tgtcgggtct tacgcgcagt gactttgaac
      301 tccttgaaga cggtacgaga caggaaatcg tccagtttga atcgtcggaa gctccctttg
      361 atctcgtact cctaatcgac gtctctggct ccacccgcga caaagtcaaa ctgattcgcg
      421 ccgctgcgct tcgttttgtt gctgcggctc gacagtccga tcgcatcgca attatctctt
      481 ttgccggtca accgacgctc gtttcaccgt tcacactaaa tcgtgagctg ttacggcaaa
      541 gagttgaggc catcgacact gcaactggtg acacaaaagt ctacgacgct atcgagtttg
      601 cactccgaca aatacccgct ggaactatag gttcaaggcg caccgctctg gtgctgatga
      661 gtgatggact cgatggctca atcgaaggcg ttcaaggtga tggctcgcag gtttccttta
      721 gcgaggtgct caaccacgta caggaatttg acggcgtgct ttattctctg tggctcaata
      781 cggaatacga agcgctgagt ccgctcgaca cgcaaccaga agctttcgac cagagtcatg
      841 agcgcatgaa aggaatggcg gtcgcgggtg gcggcgcctt ctatgaggta gaccgtctag
      901 aagatctcgc cggctcgtat gagcgcgtgg tggctgatct gggcaccgtc tacagtcttg
      961 cctatcgacc tgcaaacaaa gcgcgtgacg gcaagtggcg aacgatccgc gtcaacgtag
     1021 cgcggccctc cgcagttgcg cgaggcaaac atggctactt cgcgaactaa cctgaaaccg
     1081 tccggcagct ttttagcgct gggcgtgatt ttgattctgg cactgttgcc ggctctgact
     1141 cgtgcgcaaa gcactgagcc taatgaagtc attcgcgttg attccgacct ggttgatctc
     1201 agggtcagtg tggtgcgccg ggaccctctt aacccagtgg ctgaattaaa acagtctgac
     1261 ttcctcatac ttgaagacgg caaaccacag gagatttctt tcttcgcggg cgagaacgac
     1321 ccttttgacc tgatactgtt actcgatctt tcggggtcta ctgccaaaaa actgaagctg
     1381 gtaagaagct ctactgagcg atttgtcgaa gcgacacgtc ctatcgaccg cgttgctatt
     1441 gtgactttta ctgatgtccc acaggtcgtc tcaccgctca ccctggatcg cgaggcgttg
     1501 aagaaatcga ttaagaagat tactgaaccg agcggcggca caagcttttg ggattcgctg
     1561 cgcttcgtgc tcgattctcc agctacaaac ggcatgcgtc gccgagcggt ggtggtgatg
     1621 actgacggag tcgataacgc gcttcccggc atctttggag acgggtcgcg aacatcattc
     1681 gcagaactgt tggaactagt gcgagcttcc gatgtgattg tctttccggt ttatctggat
     1741 acggagaagg aggaaatcaa acgccatcgc agtcccgcca gtgcctatgc gacggcgcga
     1801 gaaaaccttt cgcaactagc tgaggttggt ggaaccatcg tatatacggc cagggagctc
     1861 aaagatctgg accgcgtcta cgatcaggta attaaggatc tcggcaccgt ttacagcctg
     1921 ggctaccgct caaacaacaa gtcccgggat gggaaatggc gttcagtgac agtgcaggtt
     1981 atcggccaac cacaagtcag cgcccgcact aagcgcggtt actacggccg cgctcagaac
     2041 tgaaattccg gtcccggctc cggatattgg ttgaaaatcg agccgatgag tctataatgc
     2101 ggctgcggtc acctccccag aaatgcagcc tccggtcggt tgaaaggaga ttccttccat
     2161 gcgaaaaacc tccacctcgt ttcctatcct gttttgtgcc ctcatagtca tgatggcagg
     2221 agttgcgacc gcccttgctc agaaggaagg ggcaagaggg cagacacgag atcgctttgt
     2281 tatctcggcg cgtgcggggg gcgtaaacgc cgtcaccgga aaaactggtg tgcatgcgaa
     2341 gggaagtagt atttgggaac agctgaccat caaagacaat ctcgagggcg gcgatgtagt
     2401 gaaaacagga ctggatgggc gagtggagat attgctcaat ccggggtcct acatgcgggt
     2461 cggcgagaat tcggagtttg agctaacgag caactccctg gacaatctag aagttcgcgt
     2521 tatccgcggt actgcgattg tcgaagccac cggcgccgaa gatactgaac tcttaatcaa
     2581 catcacgacg cctcacgcaa aaatggcaat cgtacgccgc ggcctctacc gggtgaatgt
     2641 cgtgcccggt gatgtgactg aggttattgt caggaagggc aggctgctct tatccgatcc
     2701 cgacgccaag atcaaagccg gcaagaaact catattcagc gggaattcct attccgtcgc
     2761 cgcgctcacc gacgctgaaa agaaagacgt ggactccttt gatctttgga gcaaagaccg
     2821 ggcgcacgtt gtagcaaaag ctaatcaaca acttacctca agggatcggc gaatcttacg
     2881 ctccggtttc accgacgatt gggcgtttcg cttctcgccc caaatcggcg gaatttggtt
     2941 cttcgactcg ctgtttggtt gctattcctt catgccattt tatgccggct ggggctcacc
     3001 gtatggcagg tcttactccc gttccttcct cggcataccg tattggcagg ttcggaatta
     3061 ttactgggcg acacaacgcg gctacttccc tgacgcatcc tcacccgtat cagtgcccac
     3121 cagcataccc ggaaagtcta ttgggactag cccgccgagc ggcagctcgt ttcctaaagg
     3181 atcacgcggc gggaaagtgc tcccctagcc gctaccggcc tcgttcgctc accaggctgt
     3241 tcagtacctt attaacaagc gccaggacct cggtgcggct gaggcctgca aggagcaggt
     3301 cggtcagtgc tgcccgcacc tttgcgtgct ggcggtcctc gactgtctga cccgtaacgt
     3361 gcgacacgac cacggcacct gacccatgcc tgatccggat caggccttcc ttctgcagtt
     3421 gccggtaggc aaaagcgatg gtgttgagat tgacgcccaa atcggcggca acctgacgca
     3481 ctgggggtaa actcgaaccc gccgccagtt ccccccgggc gattaattcc ttaataccgt
     3541 ccaccacctg acgatacatc ggccgaccgt cccgctcgtc aatggttata tacatacagg
     3601 cctcagattg tctgctgcct ctatacacca tacaatcagc cgaagtccag ggaaatcttg
     3661 ggatatggcc atctccaata aatgatcacc tctgtgtggt tgcgactcaa ctccccggca
     3721 aatccgccgc caattgagcc gtgactgggt aaatttctga cgctggcgtt acgtgaggtg
     3781 ggttttgatt agattggccg aactcgcctc ctggaaaaaa catcagcaga atacagaacc
     3841 acagaataat gaccccgggt ctgtacgcct aggtcagcgc atcgacaaac gcgccagtaa
     3901 gctggcgtgt tttaacgggc gggttatgta tatgaagttc tgggtactga gttcctcaat
     3961 gcattccctg gagacgtatc agacacctgc ggtgacggca cgatatccag cgcttgacgc
     4021 cttcttcatg gcttcaaccg cctcggcagt cgacagtaga ggagtggttt tgactgactt
     4081 gcatgcgcca cctgcggcaa aggccatggc aatcgcaccg gcgtcaacgt tactgggcat
     4141 ttgaatgatg agcaccacat catagtcgcc aaatgcgaac catgcactct cgatcttt
//
LOCUS       DASUEX010000631         3319 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1251717, whole genome shotgun sequence.
ACCESSION   DASUEX010000631 DASUEX010000000
VERSION     DASUEX010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3319
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1251717"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="VFV61_10515"
     CDS             complement(<1..518)
                     /locus_tag="VFV61_10515"
                     /inference="COORDINATES: protein motif:HMM:NF017633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HEX4900956.1"
                     /translation="MRQETLQAVVAELETTLVDHTVGRVFQTSPLSLAIDFGVRQTGY
                     LFVSAEPAQPRIHMIKRSAKELEKQSLPPALFAQALRSILAGAQLTAIAKDETERVVR
                     LRFLVEDDLGTAGFRTLVCQLTGRSANLFLLDDDDHIRHALRSPKGDGQQIGQIYLPP
                     APQPFHTSETKS"
     gene            complement(602..1615)
                     /locus_tag="VFV61_10520"
     CDS             complement(602..1615)
                     /locus_tag="VFV61_10520"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEX4900957.1"
                     /translation="MESSDVSAAHGDEPAYSGAQGSKLIEFPGASRSTVPQWRKELSE
                     RVREVQEKRAREEALDLSLEKRADLSESLPQLELLPQAEAAPVNPLVAAALRRIERAH
                     DGRVYAGNSSGSSAVAYAAPMLQDDTAVDEVAPLAATICAAEELQPHQTEKAHNLVVV
                     PTPPIVETTIVPKPKRMIGDINDPALNYLDSVCTATRLETSRYKRASVGARLVSAFVD
                     LAVVTLLCAPFAAALELTDTNWQVPRTWMLAAGVFSLVGFLYFTVSTALTGRTLGLKL
                     LSLRIVDVRTGLIPTGTQSAGRALIYQASLILLGITSVLALLNPERLTAHDRITRTAV
                     VQS"
     gene            complement(1886..2263)
                     /locus_tag="VFV61_10525"
     CDS             complement(1886..2263)
                     /locus_tag="VFV61_10525"
                     /inference="COORDINATES: protein motif:HMM:NF021486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HEX4900958.1"
                     /translation="MKLFTVAEANALLPAVREILKKIQDARRKLASYRNEAKAAAEQA
                     EQGGGGFSDGLRYADLLIDLTSQTNELEVLGVQLKDFDRGLVDFPSLRDGRVVLLCWQ
                     IGEGDEVEWWHNVEDGFAGRTPI"
     gene            complement(2289..3026)
                     /locus_tag="VFV61_10530"
     CDS             complement(2289..3026)
                     /locus_tag="VFV61_10530"
                     /inference="COORDINATES: protein motif:HMM:NF014045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEX4900959.1"
                     /translation="MHNFDPTNNISWQLGHRGLVAVFIDGNNLFHAARFHNIDIDYNK
                     LLRVLLGDGRLLRAFFYTGVDVGAERQQGFLLWMRRNGFRVIQKELKTFYDGTRKANL
                     DVEIAVDMLSLAGRYDTAVLVSGDEDFVYAVNAVAYKGCRVEIAGFRSNTAPKLIDVA
                     DYFIDLGEIADKVRKEASHAHRYDERELHEHVPAHFIHEHMQIEQESATVEFQTAMHV
                     VLETELADESENFGSEASEFIRVTDEQ"
     gene            complement(3118..>3319)
                     /locus_tag="VFV61_10535"
     CDS             complement(3118..>3319)
                     /locus_tag="VFV61_10535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900960.1"
                     /translation="NGQQVTARVGASEEVTPVELVTLVFPTSGRTDALELHLAWEVSL
                     GAGRLVYVDAVEGKVLRANLAT"
BASE COUNT          753 a          878 c          929 g          759 t
ORIGIN      
        1 tgcgacttgg tttctgaggt gtggaaaggc tgaggtgcgg gcggtaggta gatttgacct
       61 atctgctgcc cgtcgccttt gggagatctg agtgcgtggc gtatgtgatc gtcatcgtcc
      121 aggaggaaga gatttgctga acgtccagtt agctggcaca caagggttct gaaacctgcg
      181 gtacccaaat cgtcctccac aagaaatcga agacgaacga cacgttccgt ctcatcctta
      241 gctatggcgg tcaattgtgc tcccgccagg atggagcgca gtgcttgcgc gaaaagggct
      301 ggtggcagcg actgcttttc cagctctttg gcgcttctct taatcatgtg gattcgcggt
      361 tgagctggtt ctgcgcttac aaaaaggtaa ccagtctggc gaaccccgaa atcgatcgct
      421 agcgacaaag gcgacgtttg aaacacgcgg cccactgtgt ggtcaaccag cgtcgtttcc
      481 agctcggcaa cgactgcctg aagggtttcc tgacgcatcg tttgtcgagg ctaacagaat
      541 aggccaggca aaagaaaagg accctcgaaa acgagagccc tggaatcgaa tgtccgttgc
      601 cttaactctg aacgactgcc gttctggtga tgcgatcgtg ggccgtcaac ctctccggat
      661 taagcaatgc taagacggag gtaatgccca ggaggattag tgatgcctgg taaatgagcg
      721 ctcgtccagc agactgtgtg ccggtcggaa tcaggccagt tcgaacatca acgatgcgga
      781 gggaaagcag tttgagtccg agtgtccgtc cggtaagcgc ggtggaaact gtgaagtaga
      841 gaaagccgac gagtgaaaaa actccagccg caagcatcca ggtgcgcggc acttgccaat
      901 tagtgtcggt gagttccagc gcagccgcaa atggggcgca aagcaatgtg acgaccgcta
      961 agtcgacgaa cgcgcttacc aatcgagccc caaccgatgc acgcttatag cgagaagtct
     1021 caagtcgagt ggcagtgcat actgagtcta gataattaag agccggatcg tttatgtcac
     1081 caatcatccg tttcggtttg gggacaattg tggtttcaac aattggtggt gtgggaacga
     1141 ctacgagatt gtgggccttt tcagtttggt gcggctgcaa ttcctctgca gcgcagattg
     1201 tcgctgccag aggagcaacc tcgtccaccg cggtgtcgtc ttgcagcatt ggtgcggcgt
     1261 aggctaccgc agacgagccc gatgaattgc ccgcgtagac tcgtccgtca tgagcgcgct
     1321 cgatccggcg cagtgctgct gcgaccaacg gattaacggg agcggcctca gcttggggaa
     1381 gcagctctag ctgtggcagc gactcgctaa ggtccgcacg tttttcgaga gagagatcca
     1441 gggcttcctc gcgggcccgt ttctcctgta cttcgcgtac acgttcgctc aattccttac
     1501 gccactgagg aactgtgctg cgtgaagctc cgggaaactc gattaatttt gaaccttgtg
     1561 cgccgctgta ggctggctca tcaccatggg cggccgagac atcagaactt tccatgcggg
     1621 ctcctgcggt gcattggcca cattgatcca atccggattc aatgggcgcg ccgcatgaga
     1681 gacattccat accaaagtgc ctccgaaggg gaatctggag agccggattt cgcgacgcca
     1741 ctctgtctag catccagctc ggtgcacaat cggggcgcat gctatcagga actactttaa
     1801 ctggtcaatg agaattattc aggtagttga agttgccgtc cagcaggatc ggcaggattg
     1861 aatgaggctg acctgtggaa aactttcaaa ttggagtgcg tccggcgaaa ccatcttcga
     1921 cgttgtgcca ccactccact tcatcgcctt caccaatctg ccagcagagc agaaccacac
     1981 gcccgtcgcg caaagaggga aaatccacca ggccgcgatc gaagtccttc aactgcacgc
     2041 ccagaacttc gagttcattg gtttgtgagg tgagatcaat tagcagatcg gcatagcgca
     2101 agccatcgct gaaaccaccg ccgccctgtt ccgcttgctc ggcggcggct ttggcctcgt
     2161 ttcggtaaga ggcaagtttt ctgcgcgcgt cttgaatttt tttcaaaatc tcacgcacag
     2221 caggcagcaa cgcatttgct tcagcaacgg taaataactt catgagtctc tctgttttgc
     2281 tgaagccgtc actgttcgtc ggtcacgcga atgaattcag aagcttcgct gccaaaattc
     2341 tcagattcat cggcgagctc agtttccagc acgacatgca ttgctgtttg gaactcaacc
     2401 gtcgccgatt cctgctcaat ctgcatatgc tcgtggatga agtgagccgg aacgtgctca
     2461 tgcagctcgc gttcgtcgta acgatgagcg tgactggctt ccttccgcac cttgtcggcg
     2521 atttcaccga ggtcgatgaa atagtcggca acgtcgatca gtttcggcgc agtgttggag
     2581 cgaaagcctg cgatttccac gcgacagcct ttataggcaa ctgcattcac agcatagaca
     2641 aaatcttcgt cgccagagac aagcacggca gtgtcataac gtccggcaag ggacaacatg
     2701 tcgactgcta tctccacgtc gaggtttgct ttgcgcgttc cgtcgtagaa ggtcttcaac
     2761 tctttttgaa taacgcgaaa accgttgcgg cgcatccaga gcaaaaaccc ctgctgtcgt
     2821 tctgcgccga catcgacccc ggtgtagaag aaggcccgca gcagacggcc gtcaccaagc
     2881 agcactcgca gcagcttgtt gtagtcgata tcgatgttgt gaaagcgtgc ggcgtgaaaa
     2941 aggttgttcc cgtctatgaa caccgcgacc aggccgcggt gccccagttg ccaggagatg
     3001 ttgttcgtcg gatcgaagtt atgcatgaaa atagaaactc caataagttt tttattaacg
     3061 agactagcat gcgtttttgc agctttttgg tcaagctggt aaataatcag gaggcgacta
     3121 ggttgctaga tttgctcgca agactttccc ttcgactgca tccacgtaaa cgaggcgacc
     3181 ggcgccaagg ctgacttccc aggcgaggtg cagttcgagt gcatcggtac ggccgctggt
     3241 tgggaatact aacgtgacca actcaaccgg cgtgacttcc tcggacgcgc ccacgcgggc
     3301 ggttacctgt tgtccattt
//
LOCUS       DASUEX010000632         3162 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1024524, whole genome shotgun sequence.
ACCESSION   DASUEX010000632 DASUEX010000000
VERSION     DASUEX010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3162
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1024524"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1334
                     /locus_tag="VFV61_10540"
     CDS             <1..1334
                     /locus_tag="VFV61_10540"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEX4900961.1"
                     /translation="ELVAQNVQKPQAPPQIGFREQKFEPSPLDELSKLVLTPNEQNVS
                     DADVLALEQAKNNVNFGFTINPLIQQYINYYQGRGRSTMESGLRRSGQYMKMARQIFH
                     EEGVPVDITWLGQVESAWKPKAMSWAAASGLWQFIPGTGRQYGLRQTAWVDERNSFQQ
                     ATRASARYLKDLATRYNGNWELAMAAYNTGAGNVDRAIARVGTANFWQIYPYIAQETR
                     NYVPNILATILIAKNPEKYGFKGIKPDSPLAYDVVQVPNATSLQLLADATDTSIDHIR
                     ALNPHLRRDITPRGEAYNVNVPAGRSKQLVALLKRIPGDRRETARIISVVPGEDWQGI
                     SSRVGIGVAQLQAFNGGVDLKSTTKLVVPNNNVRLTKWTRASSTASPTGPTLTKIRAR
                     KGDTIARIAAARNLDANEVAKLNGVMADTELRAGQEVRLPTSAAAAPSRRR"
     gene            1475..3037
                     /locus_tag="VFV61_10545"
     CDS             1475..3037
                     /locus_tag="VFV61_10545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEX4900962.1"
                     /translation="MLFRTLSAAVYGIDADLVDIEVDLTPVRGESESPNVTIVGLPDA
                     AVRESRERIRAAITNCGFFFPIQKVTINLAPADVRKEGASFDLPIALGILGANGDLAE
                     ENNLISVLCVGELSLDGRVRPIKGALPIAILARDASIKHIILPDENAKEAAVVSGVNV
                     YPVASLRNAVELIAALKSSSPPIPLKIDPTVILVHTDHYNVDFREVRGQVSAKRALEI
                     ASAGSHNILLIGPPGSGKTMLAKRLPTILPQLEFEAALELTKIHSVAGLTGRTGLVTA
                     RPFRSPHHTLSDAGLIGGGALPRPGEVSLAHQGVLFLDELPEFDRSVLEVLRQPLEDQ
                     KVTISRAAMSLTFPASFMLAAAMNPCPCGFWNDPTRECRCSPLQIQRYVGRISGPLLD
                     RIDIHIDVPAVKFRELAGDTAPETEDSATIRARVIAARERQQQRLAGEKIFSNAAMSP
                     RMIRRHCRIDSASEHMLERAMTRLGLSARAYDRILKVSRTIADLEGTAEIRSTHVSEA
                     VGYRSLDRTYWT"
BASE COUNT          707 a          888 c          871 g          696 t
ORIGIN      
        1 cggagctggt agcgcaaaat gtgcaaaagc ctcaggcacc gccgcagatt ggatttcgcg
       61 agcagaaatt cgagccgtcg ccactcgacg aactcagcaa acttgttcta acgccaaacg
      121 aacagaatgt ttcggatgct gacgtgctgg cgctggagca ggcaaagaac aacgtcaact
      181 ttggcttcac gatcaacccg ctgattcagc aatacattaa ttattatcaa ggccgtggcc
      241 ggtccacgat ggagtctgga cttcgtcgct caggtcaata catgaagatg gcgcgccaga
      301 tcttccacga agaaggcgtg ccggttgaca tcacctggtt aggccaggtc gaaagcgcct
      361 ggaagccaaa ggcgatgtcg tgggcggctg cttcgggtct ctggcaattt attccgggta
      421 caggacgcca gtatggattg cgccaaactg catgggtgga tgaacgtaac agtttccaac
      481 aggcgactcg cgcctcagct cgttatttga aggatctggc gactcgctat aacggaaatt
      541 gggaacttgc gatggcggcc tacaacaccg gcgccggcaa cgttgatcgt gcaattgccc
      601 gcgtaggtac cgcaaatttc tggcagatct acccatacat cgcccaggaa actcggaatt
      661 acgtgcccaa tattctggcg acaatcctga tcgcgaagaa tccggaaaaa tatgggttca
      721 aagggatcaa gccggattcg cctctggcct atgacgtggt ccaggtacct aatgcaacca
      781 gtctgcagtt gcttgcagat gcaaccgaca cgagcatcga tcacattcgg gcattgaatc
      841 cccatctcag acgggacatc acgccacgag gtgaggctta caacgttaac gttccggcag
      901 gccgatcgaa acagttggtc gcattgctga aacggatacc cggtgatcgc cgcgaaaccg
      961 cgcgcatcat ttctgtggtt ccgggtgaag actggcaggg tatctccagt cgcgtgggca
     1021 tcggcgtagc gcagttgcag gcatttaatg gcggagttga tctaaaatca accaccaagc
     1081 tagtcgtccc gaataacaat gtcagactga ccaaatggac ccgtgcctca agcaccgcga
     1141 gtcccactgg tccgacgctg accaagattc gcgctcgcaa gggcgacaca attgcgcgaa
     1201 tcgctgccgc tcgtaacctc gatgcaaatg aagttgcgaa actcaatggt gtgatggcgg
     1261 atacggaact ccgtgcgggg caggaagtca gactaccaac ttcggctgca gcggcaccgt
     1321 cacgtagacg ttagctattt ggatcaactt ggcaaacgcg tctgcctcac agcgtgttta
     1381 agatgcgagc gcgctgacga gaaattcgta agcgcgctca aactgttctt gcgattacta
     1441 agctctttcc actctcacat tggagcatcc tcacatgctt tttcgtaccc tttcagcggc
     1501 cgtttacggt atcgatgcag acctcgtaga cattgaagtc gatctcactc cggttcgtgg
     1561 cgaatcggaa tctcccaacg tcaccatcgt tgggctgcct gatgcggccg tcagagaatc
     1621 tcgcgagcgg atccgcgccg ccatcacaaa ctgcggtttc ttttttccga ttcaaaaggt
     1681 cacgatcaac ctcgcccccg ccgatgtgcg taaggaaggc gcgagctttg acttgccgat
     1741 tgcattgggg atcctcggtg ctaacgggga tctcgctgaa gagaacaacc tgatcagcgt
     1801 gctgtgcgtc ggagagcttt ctctcgatgg acgcgtacgg ccgatcaaag gtgcactacc
     1861 aatcgcgata ctggcgcggg atgcgtcaat caagcacatc atattgccgg atgagaatgc
     1921 gaaagaggcg gccgttgttt cgggtgtcaa cgtttacccc gtcgcgagtc tccgcaatgc
     1981 cgttgaatta atcgccgcat tgaagtcttc gagcccgccg ataccgctga agattgatcc
     2041 caccgtcatt ctcgttcata cggatcacta caacgttgac tttcgcgaag ttcggggtca
     2101 ggtctcggca aagcgagcac ttgaaatcgc cagcgctggt tcacataaca tcttgctcat
     2161 tgggccacca ggctctggca agaccatgtt ggccaaaaga ttgccgacaa ttctgccgca
     2221 gcttgaattt gaagccgcac ttgagctaac gaagatacat tcggttgcgg gactcactgg
     2281 tcgtacgggc ctggtgactg ctcggccctt tcgttctcct caccacacac tcagcgacgc
     2341 tggcttgatc ggaggaggtg ctcttccccg gcctggcgaa gtctcgctcg ctcaccaggg
     2401 cgtactgttt ctggatgagc tcccggaatt tgatcgcagc gttctcgaag tgctgcgcca
     2461 gccattggaa gatcaaaagg tgactatcag ccgggccgcc atgtcgctga ccttcccagc
     2521 atcgttcatg cttgcggctg caatgaatcc ctgtccctgt ggattttgga atgacccaac
     2581 gcgcgaatgc cgttgttcgc ccttgcagat tcaaagatat gttggtcgca tttccgggcc
     2641 actcctggat cggatcgata tccatatcga tgtgccggcc gtgaagtttc gtgagctggc
     2701 tggcgatact gctcctgaga cggaagattc tgcgaccatc cgggcccgcg taatcgccgc
     2761 ccgcgaacga cagcagcagc gtctcgcggg cgagaagatt ttctcaaatg cggcgatgtc
     2821 cccgcgcatg atccggcgtc actgccgcat cgactctgcc agcgaacata tgctggaaag
     2881 ggctatgacg cgcctgggtt tatcggctcg cgcctatgac cgcattctga aggtgagtcg
     2941 cacgatcgcc gatttggaag gtactgctga gatccgctca acccacgttt cggaagcggt
     3001 gggctatcgt tcccttgacc gcacgtattg gacgtaggca gggttccctt ccaccgggcg
     3061 ttgataaggg tccaggggtt gttcacccgg ccgccggtta gccatctgcg agcgagccag
     3121 aaagccatcc agacaccaac ggcagttagc catcgttcaa tt
//
LOCUS       DASUEX010000633         3091 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_195550, whole genome shotgun sequence.
ACCESSION   DASUEX010000633 DASUEX010000000
VERSION     DASUEX010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3091
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195550"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(205..606)
                     /locus_tag="VFV61_10550"
     CDS             complement(205..606)
                     /locus_tag="VFV61_10550"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX4900963.1"
                     /translation="MSHDNVDIVRSLYDSFGQGDVPSVLGKMDQGIEWNEAESFIYAD
                     RNPYMGPQAVLEGVFMRLGTEWEGFSVTPEEWMDAGNRVVVLGTYAGTHRGTSKRVRA
                     QFAHVWSLKEGRVVRFQQYTDTKQFADVVTH"
     gene            complement(630..2213)
                     /locus_tag="VFV61_10555"
     CDS             complement(630..2213)
                     /locus_tag="VFV61_10555"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEX4900964.1"
                     /translation="MRSETVKLQAIAPSTVRVPGKAPRWVMPAVKIALLVADIFIAVS
                     AFAGAFYLRESRQLWQFAAGSFSWSPGFAPYALLLPFVVVTRLLMIRYYDLYRLRGEF
                     SFVEDAVRLFKSTAIGSLLIVAAAFLYRGGFEYRAFSYARSVFLLDFLLALAGIVLVR
                     LLVRSAQTVARGRGINLIPTLVVGRGAEAALCIKEMRERPALGYRVIGVVEATQTNRL
                     HKGSETDLSKGAIFFEGVPVVADLKQLPEAIRDAGANEVIIADTSVDGDALFEVMMRC
                     GRRRGVEFRIAPNLFNCLPRKTEIDQIGALPMITLFREPLSNSARLLKRSSDLAIAAL
                     TLLLAAPIWLLIALLIKLDSRGPVFYLQERVGMDGRMFLCYKFRTMHPGADSKVHRDY
                     QKQFIAGSAEANLGNGAQPVYKFQSDARITRVGRWLRRASLDEVPQLFNVLRGDMSVV
                     GPRPPIPYEVEDYELWHRKRLDMKPGLTGLWQVSGRNRIPFEEMVKLDLYYIENWSLL
                     LDLKIIMRTALVIIRGDGY"
     gene            complement(2243..>3091)
                     /locus_tag="VFV61_10560"
     CDS             complement(2243..>3091)
                     /locus_tag="VFV61_10560"
                     /inference="COORDINATES: protein motif:HMM:NF015940.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF354 domain-containing protein"
                     /protein_id="HEX4900965.1"
                     /translation="HGGRELTAKAGNLFQRAWALRAWASGRSFDLAVSHNSYSQILAA
                     RGLGVRCVTLMDYEHQPANHLAFRLASTVLVPRAFPDEDLSRYGAGAIKVKRYNGIKE
                     DVYLADFENDPAFAGKLREIGAKLESTIVVVRPPARDALYHRFENDLFAGLLKRLSET
                     PEVTTILLPRNAGQRTTYAAWVGPSTIMPEEPLDGANLIAASDLVVSAGGTMNREAAA
                     LGVPAATIYAGNWAAIDEELVREGRLIRLSTPADIESLNLQKKPKTESRNAVSVKKEV
                     ADLILG"
BASE COUNT          770 a          909 c          773 g          639 t
ORIGIN      
        1 aagtatttaa aatgactccg aattgatagc aagtcgttac attacgggcg tgagactcac
       61 gatttcgggc aaaaacgacc aggaaaaaag gcaaccagga tccaagcccc ggctcagatt
      121 ggtaaccaat ggtctccttc agaactcaaa acaaagaccc aaaatcaaag gccaaagacc
      181 aataaaggca aacttacgcc agaactagtg agtaacgaca tccgcaaact gcttcgtatc
      241 ggtgtattgc tgaaagcgca ccactctgcc ttctttcaaa ctccatacgt gtgcaaactg
      301 ggcccgcacg cgcttgcttg tcccgcgatg ggttcctgca taagtgccta aaactacgac
      361 gcggtttcct gcgtccatcc attcctccgg cgtcacggaa aagccttccc actcagttcc
      421 gagtcgcata aagacgccct caagcaccgc ctggggtccc atatagggat tgcgatctgc
      481 gtaaatgaag ctttccgctt cgttccactc aattccctga tccattttgc ctaacactga
      541 aggaacatcg ccctggccga atgaatcgta caaactgcgg acaatatcaa cgttatcgtg
      601 agacatctgt ttctcctttt atggcgcggc taatagccat cgccgcgaat gattactaag
      661 gccgtgcgca tgatgatctt cagatccaga agcagcgacc agttttcgat gtagtaaaga
      721 tccagtttca ccatctcttc aaagggaatg cgattgcggc ccgatacctg ccagagacca
      781 gtaagtcccg gtttcatatc caacctcttc cggtgccaga gttcatagtc ttctacctca
      841 tagggaatcg gcggccgcgg accaacgaca ctcatatcac cgcggaggac attgaacagt
      901 tgcgggactt cgtccagact cgcgcgtctt aaccagcgtc ccacgcgtgt gatgcgcgca
      961 tcactttgaa acttgtaaac cggctgcgca ccatttccca gattcgcctc cgcgctgccg
     1021 gcgatgaatt gcttttgata gtcgcgatgt accttgctgt cagcaccggg atgcatcgtg
     1081 cgaaacttgt aacaaagaaa catccggcca tccatgccaa cccgttcctg caaatagaaa
     1141 acggggccgc gcgagtctaa tttgattagc aacgcaatca ataaccaaat cggggcggcc
     1201 agcaggaggg tgagggcggc gattgccagg tctgagcttc gtttcagcag ccgggcagaa
     1261 tttgaaagcg gttcgcgaaa tagcgtgatc atcgggagcg cgcctatctg atcgatttca
     1321 gttttgcgcg gcaggcagtt gaagagattt ggagcgatgc gaaactccac gccgcggcgg
     1381 cggccgcaac gcatcatcac ttcaaacaga gcgtcgccat cgacactggt gtccgctatg
     1441 atcacctcat tagctccggc atcgcgaatt gcttccggaa gttgcttaag gtcggcgacc
     1501 accggtacgc cctcgaaaaa aatggcgcct ttgctcaaat ccgtttcgct tcctttgtgc
     1561 aaacggttag tttgcgtggc ttccaccacg ccaatgacac gataaccaag cgctggtcgt
     1621 tcgcgcattt ccttgataca aagcgcggct tctgcgccgc ggccaaccac gagagttggt
     1681 atcaaattga ttccgcgacc gcgcgcgaca gtctgcgcac tgcgcaccag caaccgcacc
     1741 aaaacgatcc cggccaaagc caacagaaaa tcaagcagaa aaacggagcg cgcatacgag
     1801 aaagcgcgat actcaaaacc accgcgatag aggaaggcag ccgcgactat caacaacgac
     1861 cctatcgccg tggacttgaa cagccgcacc gcgtcttcaa caaaggaaaa ctcgccgcgc
     1921 agacggtaaa ggtcgtagta ccgaatcatg agcagacgcg tgactaccac aaatggcagc
     1981 aacaacgcat atggcgcgaa accaggactc caactgaaac ttccagcggc aaattgccag
     2041 agttgccgcg actcgcgcag atagaaggcg ccagcgaaag cgctcacggc aataaatatg
     2101 tcagcgacaa gcaacgcaat cttcaccgcg ggcatgaccc agcgcggggc cttgccggga
     2161 acacgcacgg tgctcggagc aattgcctgc agtttgacag tttcggatct catttaaagt
     2221 ggcaaagcgg cttggctgcc agctaaccca gaatcaaatc ggctacttct ttctttaccg
     2281 aaacggcatt ccgagattca gttttcggct tcttctgcaa gtttaagctt tcgatatctg
     2341 ccggggtgct gagccgaatc aatcgtccct cacgcacaag ttcttcatca atcgcggccc
     2401 aattgccagc atagatcgtg gccgcaggga cgcccaaggc agcagcctcg cgattcatcg
     2461 tgccacccgc gctcaccacc aaatcgctgg cggcaatcaa gttcgctcca tccaaaggtt
     2521 cctcaggcat gatcgtgctc gggccaaccc atgctgcata ggttgttcgt tggccggcat
     2581 tacgcggaag caggattgtt gtcacctcag gcgtctcact caatcgtttt agcaaccctg
     2641 cgaacaaatc attctcgaac cggtgataga gagcatcacg cgccggcggt ctgactacga
     2701 cgatcgtgct ctcgagcttt gcgccgatct cgcgcagctt tcccgcgaac gcaggatcat
     2761 tttcaaagtc agccaggtag acgtcctctt tgatgccatt gtaccgctta actttaatag
     2821 ctccggcccc gtaacggctg agatcttcat ctggaaaggc gcgcggcacg agcaccgtag
     2881 atgcgagacg aaacgccagg tgattcgccg gctggtgctc gtagtccatc agcgtgacgc
     2941 agcgcacacc cagtccgcgc gccgccagga tctgggaata ggagttgtga ctaacggcca
     3001 gatcgaaaga tcgtccgctg gcccaggctc gcaatgccca agcccgctga aataggtttc
     3061 ccgctttggc tgtaagctcg cgtccaccat g
//
LOCUS       DASUEX010000634         6376 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1252192, whole genome shotgun sequence.
ACCESSION   DASUEX010000634 DASUEX010000000
VERSION     DASUEX010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6376
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1252192"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            365..2050
                     /locus_tag="VFV61_10565"
     CDS             365..2050
                     /locus_tag="VFV61_10565"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEX4900966.1"
                     /translation="MKNHPCLLTLTFVLVSCCCTSVDAQRQLVRSSSSGGPLMPEQAA
                     YDVKSYDLNLKIDPEAQTIKGALTAYARIVQPLDWFVLDLDPPLVVDSVNMVNAAGKP
                     LEQLRFERREGKLWIAFPKTKKAGEDVRVRIAYGGKPRVAPRPPWVGGFAWSKTKSGD
                     HWFTVTCQNDGADIWFPVKDHPSDKPETTDLHYTVPQPLIAVANGRLQNVVKNEDGTQ
                     TFNWHISQPINNYSITLNVAPYKTIEDKYTSSSGGEVPVIFWVLPEDYDKGFEVVKQT
                     KLYLRFLEEYLGPYPFRAEKVGIVHTNHLGMEHQTIIAYGSTFKLNEFGFDWLMLHEL
                     GHEWWANLVTASDWRDFWIHEGFQSFMDTFYLEKVAGKDAYRKAMAGRMSHLRNKQSV
                     APRESRTTTQMYMAAPDYVASDGDIYNKGAVILHTLRYLIGEQAFLKSLHRMAYPDPR
                     MEKVTNGKQVHFATTDDFLHIAERESGADLDWFFEVYLRQPKLPRLITEKTDTQVTLR
                     WESPDGLPFPMPVEVQIGDKTKRYEMPKGSVTIPLAAGQAMVVDPQGWILKAQ"
     gene            2069..3283
                     /locus_tag="VFV61_10570"
     CDS             2069..3283
                     /locus_tag="VFV61_10570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900967.1"
                     /translation="MFRVVVLSIIIFIPIGSKAQTAVSHPVNLTPHRITLTKGRSAGS
                     FALRLPKDFAISVAVEGLKRVRFMAKSPDGRIFVTDMFNLTDNKQGVVYILDQFDPST
                     RSFKKVTTYLRNLRNPNSVAFYTDPQGVHWLYLALTDRLVRYRFTKGETAPTSAPEVL
                     ATFPDYGLSYKYGGWHLTRTIAIGGNGKIYISVGSSCNACEEKEEVRASVIEMDPDGK
                     NQKHFARGLRNAVGLRWIEGQLVATNMGSDHLGDDRPADTMYALQGGTNYGWPYCYQS
                     GPNRFPDPKFNARSSKLNCRNVPVAYAAFAAHSSPLGFEYFDANSSGELRDSFLVGLH
                     GSTKENLNRGYRVVRLRGRHARSTTPENFIDGFLEGKKIHGRPCDVLKVGNDSFLLTD
                     DYAGVVYYVYKK"
     gene            3654..4073
                     /locus_tag="VFV61_10575"
     CDS             3654..4073
                     /locus_tag="VFV61_10575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900968.1"
                     /translation="MRADQLQSWVLRLTGATEVLAFISVVMPRAWMESAHSWLGLGAM
                     PEGPIIMFMIRQASYAYGMHGVSLWIMATDVRRYQRLIVFNAISFLVAAVVFSLIDYT
                     AGMPFWWTIVDAVGCASFGAAVLILSSRMRESLNHER"
     gene            4063..5241
                     /locus_tag="VFV61_10580"
     CDS             4063..5241
                     /locus_tag="VFV61_10580"
                     /inference="COORDINATES: protein motif:HMM:NF044863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900969.1"
                     /translation="MSVETENHLVNDTRSAYEAAHVVAASVETHFSRHLAAARERGED
                     ELASQPDAQTIETMIDAAFWASLRHEEGNSPKISLAFLPPEQTVQPIVFGKSLPLIPR
                     TLTKLAPAVERPGIHYGVWFDGGELRIWGATRKVPSFCFVLEVIEPGLLVVKHRRLDG
                     FGKFANVAVLKGKRVKIVDEKGTSLPDCPALLTALLAFTSPEEWSRDHNSVNILVQLA
                     ASMRAHGHGGSLLVVPAGDDGWRESIVHPIQYAISPPYTGLSHLLGQQATEENHLWDA
                     SLRKAVDTLGGLTAVDGATVINEHYDALAFGVKIRRAGGNAPVEQMVITEPIVGDVAE
                     VVHPAFNGGTRHLSAAQFVYDQRDALAMVASQDGRFTVFAWSPCEQMVHAHRVDSLLL
                     "
     gene            5610..>6376
                     /locus_tag="VFV61_10585"
     CDS             5610..>6376
                     /locus_tag="VFV61_10585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900970.1"
                     /translation="MSNKLKKNRPVYRVFANEEGQAFLRLEPANVTVPIERPVTLKKL
                     EQAKAEAINRRNNLEDVEQEQRRLTTDPRRAETEKEIEVALKPLRKGPWLGRAEYATS
                     YFLRRISVFWGALVMIAILYKVVQYSSEHFPEAAHRFVLGLFLLGLAIALIVIGTEEN
                     RKKYRKIIVGLFGPYGMLVLPCLLLVTAGAVLASITFRLYTRGLIALEMCSGRPVTEA
                     GLLDFFMWHFVNIVPTLQVTKLWRWGEPYCYTQSRVG"
BASE COUNT         1475 a         1669 c         1746 g         1486 t
ORIGIN      
        1 cgctcagagg cgaaaacaaa cgacaccttt cacctaactc gatagccaaa ggttacggat
       61 gctggttgag gaattcttga ggttggttcc gtggagtggg gaagaaaggg ttaaggcagt
      121 tgttttcagt gatttaatct atttttccaa aaatgattta gcttgactgc ctccatggtg
      181 tgatacgcat aaggcaatgc tggcaccggg gccgtctttg attggctatt gccgattcgc
      241 gacgttctat tcattaaggc tgataggcaa tcggcaattg gcaatcgcca atcggcagtc
      301 tctcaaagtt atctggacag atatcgctgc cgaaatctat gatcggttcc ggaaccttac
      361 aactatgaaa aaccatcctt gccttttgac gcttaccttt gtcctggtca gttgctgctg
      421 tacgtccgtc gatgcccaac gccagctagt acgcagcagc agctcgggcg gtcccctcat
      481 gccggaacaa gcggcttacg atgtgaagtc ttatgacctc aatctaaaaa tcgatcccga
      541 agcacaaaca atcaaaggtg cattgaccgc atacgcacgg attgtgcagc cgctcgattg
      601 gttcgtcttg gatctcgatc cgcccctcgt agtcgattcc gtaaatatgg ttaacgctgc
      661 cggtaagcct ctcgagcaac tgcgatttga acgacgtgaa ggaaaactgt ggattgcttt
      721 cccaaaaaca aagaaagcgg gcgaagacgt gcgggtgcga atcgcttatg gagggaagcc
      781 gcgagtggcg ccgcgcccgc cctgggtcgg gggcttcgcg tggtccaaaa ccaagagtgg
      841 cgatcattgg ttcacggtca cttgccagaa tgatggcgcc gacatctggt ttcccgtcaa
      901 agaccatccc tcagacaagc ctgagaccac cgatcttcat tacacggtac cgcaaccatt
      961 gattgctgtg gcgaatggcc gtttgcaaaa cgttgtcaag aatgaggacg gcacacagac
     1021 gtttaactgg cacatctcgc aacccatcaa caactacagc attactctta atgtcgcgcc
     1081 ctataagacg atcgaagaca agtacacgag cagcagcggc ggcgaagtac cggtgatctt
     1141 ctgggtctta ccggaggatt acgacaaagg ctttgaggtg gtaaagcaga cgaagttgta
     1201 tctgcgtttc ctcgaagaat atctcgggcc ctatcccttt cgcgctgaga aggtgggaat
     1261 tgtccatacc aatcacctgg gcatggaaca ccaaaccatc attgcctacg gcagcacttt
     1321 caaactcaat gaatttggtt ttgactggct catgctccac gagctcggcc acgagtggtg
     1381 ggcaaacctg gtgactgcga gcgactggcg cgacttctgg attcatgaag gcttccagag
     1441 ttttatggat acgttttatc tcgagaaggt ggctggcaag gatgcatatc ggaaggcaat
     1501 ggctggacgg atgagtcacc tgcgcaacaa gcagtccgtg gccccccgcg agtcgcgcac
     1561 gacgacgcag atgtatatgg cagcgccgga ctatgtggcc agtgacggcg acatctacaa
     1621 caaaggcgcg gtcatcctgc atacgctgcg atatctcatt ggcgagcagg cttttcttaa
     1681 gtcgcttcac cgaatggcct atcccgatcc gcgcatggaa aaggtaacca acggcaagca
     1741 ggtgcacttc gcgaccactg acgacttttt gcacattgcc gaaagagagt caggagcgga
     1801 tcttgattgg ttctttgaag tttacctgcg gcagccaaag ttaccgcggt tgataactga
     1861 gaagacggat acccaagtta cgctgcgttg ggaatcgcct gatgggctac cgtttcccat
     1921 gccggttgaa gtgcagatcg gagacaagac gaaacgatac gagatgccga agggaagcgt
     1981 gacgataccg ctagctgccg ggcaagctat ggttgtggat ccgcaaggtt ggattctgaa
     2041 agcacagtaa agcaggatct atcaaatcat gtttcgcgtc gttgtcttgt caattattat
     2101 cttcatccca attgggtcta aggctcagac tgcggttagt caccccgtca atctcacgcc
     2161 ccatcgcatt acgctgacca aaggcagaag cgctgggtcg ttcgctttgc gtctgccaaa
     2221 ggacttcgcc atcagcgtgg cagttgaagg attgaaacgc gtccggttca tggcgaagag
     2281 tcccgatggc cgcatcttcg tcaccgacat gttcaacctc accgataaca aacaaggtgt
     2341 ggtttacatt cttgaccagt tcgacccgtc taccagaagt ttcaagaaag ttacgactta
     2401 tctgaggaac ctgcgtaacc ccaacagtgt tgctttctac accgatccgc aaggcgttca
     2461 ttggctgtac ctggccctaa cagaccggct ggttcgttac cgatttacga aaggtgaaac
     2521 tgcgccaact tcggcgccgg aagtgctcgc cacttttcca gactatggct tgagttacaa
     2581 atatggtggc tggcacctga cacgcaccat cgccattggt ggaaacggaa aaatctacat
     2641 ttcagtgggc agcagttgta acgcctgcga agagaaggaa gaagtgcgcg cgagcgttat
     2701 tgaaatggat ccagacggta agaaccaaaa acactttgcg cgcggcttac gcaacgcggt
     2761 ggggttgcgt tggatcgagg gacaattggt cgccactaac atgggttcgg accatctcgg
     2821 cgatgacagg cctgcggaca cgatgtacgc gctgcaaggc ggcacgaact atggctggcc
     2881 ctactgttat caatcgggac caaaccgctt tcccgatcca aagttcaatg cgcgcagcag
     2941 caagttgaac tgtcgcaacg tgcccgtcgc ttatgctgct tttgcggcgc acagctcgcc
     3001 tctggggttc gagtactttg atgctaatag cagcggcgaa ctccgcgatt catttctcgt
     3061 tgggcttcat ggctcgacca aagaaaacct taaccgtggt tatcgcgtgg tacggcttcg
     3121 cggccggcat gcccgcagca ccacgcctga aaacttcatc gacgggtttc tcgaaggaaa
     3181 gaagattcac ggccgccctt gcgacgtatt gaaagtcggg aacgactcgt ttcttctaac
     3241 tgacgactac gccggtgttg tctattatgt ttataagaag taggtgacga gggtgggtat
     3301 cgggtgccag gtgaggggtg tcggctttcc ggattcgggg tcaggtcgcg cctgtcgtgg
     3361 gtgtgagagc cggtacagta cgggagcagt agcgaccgca ttcaacggca tcgtccttaa
     3421 ccatcgagga tcatgcgaca agttctaaaa ccttctgtca tgcttgctgc ccaaggctaa
     3481 tgcgatcgct accgctccgt actgtaccga ctcgaatcat tcgacacctg acacccgact
     3541 tggcgtgaag cgggcccaag cccacctgcc tgactgagca ttcaatttaa gactgagctg
     3601 ggtgcgactg gagacctgag acttgagacc tgagacctga gacctgagac tttatgagag
     3661 ccgatcagct tcaatcatgg gtgctgcgct tgaccggggc gaccgaggta ctcgcattta
     3721 tctccgtggt tatgccccgc gcctggatgg agtctgctca cagttggttg ggactaggtg
     3781 cgatgcctga gggtcccatc atcatgttca tgatccgtca ggcgtcttat gcttacggca
     3841 tgcatggagt atctttgtgg ataatggcca cggacgtcag acgctatcaa cggttaattg
     3901 tctttaacgc catctctttc ctggttgccg ccgtggtatt ttcgttgatc gactacacgg
     3961 caggtatgcc attctggtgg acaatagttg atgcggttgg gtgcgcttct ttcggggcag
     4021 cggtgttaat cttaagcagc cgtatgcgcg aatcactgaa tcatgagcgt tgagactgaa
     4081 aatcatctgg ttaacgatac ccggtcggcg tacgaagctg cgcacgtcgt ggccgcttca
     4141 gtggaaacgc atttctcgcg tcatctcgca gcggcgcgtg aacgcggcga agacgagtta
     4201 gcttctcaac ctgacgccca aacgatcgag accatgatcg acgctgcctt ctgggccagc
     4261 ctgcgtcatg aagaaggcaa ctctcccaag atctccctcg cctttctgcc gcccgaacaa
     4321 acggtgcaac cgatagtttt tggcaagagt ctgccgctga ttccgcgaac gttaaccaaa
     4381 ctcgctcctg cggtcgagcg accgggaatt cactatggcg tttggttcga tgggggtgaa
     4441 ctacgtattt ggggggcaac gcgtaaagtt cccagttttt gtttcgtgtt ggaagtcatc
     4501 gagcctggtc tgctcgtcgt caaacatcga cgtctcgatg gttttggaaa gtttgccaac
     4561 gtcgccgtgc tcaagggtaa gcgcgtcaag attgtcgatg agaaaggcac gagtttgccc
     4621 gattgtccgg ccctgctaac ggcattgctg gccttcactt cacccgagga gtggagtagg
     4681 gatcacaact cagtcaacat tctggtccag ctcgccgcct cgatgcgcgc ccacgggcac
     4741 ggtggctcgc tgttggtcgt gccggcgggc gatgatggtt ggcgtgaatc aatcgtgcac
     4801 ccgatccaat acgcgatctc accgccatac acaggtctct ctcacctgct tggtcagcag
     4861 gcgaccgaag aaaaccatct ttgggacgct tcgctgcgga aagcagtgga caccttgggt
     4921 ggattaaccg cggtcgacgg ggccacggtg atcaatgagc attacgacgc gctcgccttc
     4981 ggagtcaaga ttcgtcgcgc tggcggcaat gcaccggttg agcagatggt gatcacggaa
     5041 ccaattgtgg gtgacgttgc cgaagtagtc catccagcct ttaacggagg cacgcgtcat
     5101 ctgtcggcgg cccagtttgt ttacgatcaa cgcgatgcct tagcgatggt cgcctcgcag
     5161 gatggacgct tcacggtttt cgcctggtcg ccttgcgagc aaatggtgca cgctcaccgc
     5221 gtagattcac tgctgctgta gcgcaagctt gagcgccgcg gaacttaaga agatcatttt
     5281 ccatatctca ttgctcattc tcgaattcaa ttttcaagtg caacacgcag gcccgaaggg
     5341 ccgatagtta atagccacgc ccggtagggc gtggaaaaag cgattaaatt gattcaggtc
     5401 cgaaggaccg gccgacgatt gaggggctca aataactgtc gcaccttcgg cgcttttgtt
     5461 ttattgccac cgatgtccac gccctaacgg gcgtggctat taactatcgg cccttcgggc
     5521 ctaaatgttg cacttccaac ttgagtccac cattcgtcat cggcttcaaa gttcaaagaa
     5581 ccaagctcaa agatcgtctt taaggagaca tgtccaacaa acttaagaaa aatcgaccgg
     5641 tctaccgtgt cttcgcaaac gaggaaggac aggcgtttct tcggcttgaa ccagccaatg
     5701 tcaccgttcc cattgaacga cccgtaaccc ttaagaagct ggagcaggca aaagctgaag
     5761 caatcaaccg cagaaacaac ctcgaagatg tcgagcaaga gcaaaggcgg ctaaccacgg
     5821 atccacgccg tgcggaaaca gagaaagaaa tcgaggtggc gttgaagcca ttgcgcaagg
     5881 ggccatggtt gggaagggcc gagtacgcca ccagctattt tctgagacgc atctcggtct
     5941 tttggggtgc cctagtcatg atcgcgatcc tttacaaggt ggtgcagtac tccagcgaac
     6001 attttccaga agcagcgcat aggtttgtgc tgggcctttt cttattagga ctcgccatcg
     6061 cactaatcgt gatcgggacc gaggaaaacc gtaaaaagta tcgcaagatc attgtcggcc
     6121 tgtttggacc ttatggaatg ttggtgctgc cttgcctcct cttagttacc gccggtgctg
     6181 tgctcgcatc catcaccttc aggctctata cacgaggact gatcgcctta gagatgtgct
     6241 ctggtcgccc cgtaaccgaa gcaggcctgc tcgatttctt tatgtggcac ttcgtaaata
     6301 ttgttcccac gctgcaggta accaaactct ggcgttgggg tgagccgtac tgttataccc
     6361 agagccgagt cggctt
//
LOCUS       DASUEX010000635         2186 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_450311, whole genome shotgun sequence.
ACCESSION   DASUEX010000635 DASUEX010000000
VERSION     DASUEX010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450311"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2186)
                     /locus_tag="VFV61_10590"
     CDS             complement(<1..>2186)
                     /locus_tag="VFV61_10590"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX4900971.1"
                     /translation="SMLGRTISHYRIVEKLGEGGMGVVYIAEDTLLGRRVAIKTLTSR
                     GSDNKHFRTRFLREARAVSALSHPHIAHIYDYGETEDHEPYIVMELIKGETLSDLIVK
                     GKLTIPRAIEIIKQVGEALGEAHRHGIIHRDIKPQNVAINERGSVKVLDFGLAKQLDP
                     DPSNVDDSNQGTLLTMQTREGMIVGTPLYLSPEQAMGVEVDTRSDLFALGSLLYECIA
                     GKPPFDARSPAEICAKIIRDDPTPPSTLNPDVPKELDRIVLKGLAKKPQARYQTAEEM
                     IDDLESTHSRIVGLDRTVTRMIVPQHGTHPTGALATLSDIFRRPRLPIGYVAAGLVGL
                     VVLAFGVWYLLGARPHQPTPEALQLYNTGTNAIRAGSFFQASKALELATRADDQFALA
                     HARLAEAYMELDYFDKAKDEILRASELTPDRSVLPQVDNLYLEGLTATVRRNFSGAVA
                     AYTQLAKVDPAKPEAHVDLGRAFEKNNQADKAIESYQEAIKRDSQVASAFLRLGILYG
                     RKRDLDASNRAFDKAEEIYQSLGNVEGRTEVAYQRGVVLNDSIGKPDDARVRLEQARE
                     MARVVNAPYQQIRILFQLSSVALKQNKGGEAKQFATQALELAQANQMETMIARGYIEL
                     GYVDFLTADYASAEHDFQQGLDYAQKFGARLNEARAQLSLASLYEQRGEPDKALTYQE
                     PAQKFYQSAGYRTETNQAMNIRARLYRRKGDFKSALAAFEDQLKFA"
BASE COUNT          424 a          610 c          625 g          527 t
ORIGIN      
        1 agcgaacttg agttgatctt cgaaagcagc caacgctgac ttgaagtcgc ccttccgccg
       61 gtacagtcgt gcgcggatgt tcatcgcctg gttcgtttca gtccgatagc cggcgctttg
      121 ataaaacttt tgggctggtt cctgataagt cagcgctttg tcgggttcgc cgcgttgttc
      181 gtacagactc gcaagcgaca actgcgcgcg tgcctcattc aggcgcgccc cgaacttttg
      241 cgcatagtcc agcccctgtt ggaaatcgtg ctctgcgctc gcataatcgg cggtgagaaa
      301 atcgacatac ccgagttcga tgtagccgcg ggcaatcatc gtctccattt ggtttgcttg
      361 cgcaagttcg agcgcctgcg tcgcaaactg cttggcctcg ccccctttgt tttgtttcag
      421 cgcgacgctg ctgagctgga agagaatgcg aatctgttga tagggcgcat tcacgactct
      481 tgccatttcg cgcgcctgtt ccagccgcac gcgtgcgtcg tcgggtttac cgatgctgtc
      541 attcagcacc acgccgcgct gataggcaac ctctgtacgc ccttcgacat tgccaagtga
      601 ttggtagatc tcttcggctt tgtcgaacgc gcggttagac gcgtcgaggt cgcgtttgcg
      661 accgtaaagt atgccgagcc gtaaaaatgc cgaagccact tgcgaatcgc gcttgatagc
      721 ctcctggtaa ctttcgatag ctttgtcggc ttggttgttc ttttcgaatg cgcggccgag
      781 atcgacatgc gcctcgggct ttgccggatc gacttttgca agttgagtat aagctgcgac
      841 tgcgccgctg aaatttctcc gcacggtcgc ggtaagccct tcgagataga ggttatcgac
      901 ctgaggaagc actgagcggt ccggcgtcaa ctctgaagca cgcaatattt cgtccttggc
      961 tttgtcgaag taatccaatt ccatgtaagc ctcggcaaga cgagcatgtg ccagggcaaa
     1021 ctggtcatca gcgcgtgtcg cgagttcaag tgctttgctg gcctggaaaa aagatccggc
     1081 tcggatggca ttagtgccgg tgttatatag ttgcagcgct tcgggcgtgg gctgatgcgg
     1141 tcgtgctccc aacaagtacc acacgccgaa cgccaacacc acgagcccga ccaggccggc
     1201 agcaacataa ccaattggca acctcggccg gcggaaaata tctgaaagcg ttgccaaagc
     1261 tccagtcgga tgtgtgccgt gttgggggac gatcatgcgc gtcaccgtcc gatcgagacc
     1321 tacgatgcgt gagtgcgtgc tttccaggtc gtcgatcatt tcctcggccg tctggtagcg
     1381 agcttgtggt ttcttcgcca aacccttaag cacgatgcga tcgagttctt ttggcacgtc
     1441 tggattcaac gtcgaagggg gcgtcgggtc gtcgcgaatg atctttgcgc agatttcggc
     1501 ggggctgcgc gcgtcaaaag gcggcttgcc ggcgatgcat tcgtacagca gactgcccag
     1561 cgcaaataga tcgcttcgcg tgtccacttc gacacccatc gcttgttcag gggacaggta
     1621 cagcggggtg ccgacgatca tcccttcccg tgtttgcata gtcagcaggg ttccctggtt
     1681 ggaatcgtcc acgttactcg gatccggatc gagctgtttc gctaacccga agtcgagcac
     1741 tttcacgctg ccccgttcgt tgattgccac attctgcggc ttaatgtcac ggtgaatgat
     1801 gccgtgacga tgcgcctcac caagggcctc gccaacctgt ttgatgattt caatggcgcg
     1861 cggaatcgtc agcttgcctt tgacgattag gtcgctgaga gtctcgcctt tgataagttc
     1921 catcacgata tacggctcgt ggtcttcagt ttcaccgtag tcgtagatgt gagcgatgtg
     1981 tggatgggag agagctgaaa cagcgcgcgc ctcgcgcaaa aaccgcgtcc ggaaatgctt
     2041 attgtcactg ccgcgcgaag tgagtgtttt aatcgcaacg cggcggccca gcagggtgtc
     2101 ctccgcaata tacacgacac ccatcccgcc ctcgccgagc ttctcaacga tgcgataatg
     2161 agagatggtg cggccgagca tggact
//
LOCUS       DASUEX010000636         2247 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1703292, whole genome shotgun sequence.
ACCESSION   DASUEX010000636 DASUEX010000000
VERSION     DASUEX010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1703292"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="VFV61_10595"
     CDS             <1..588
                     /locus_tag="VFV61_10595"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEX4900972.1"
                     /translation="MALAAAAIGNMEGKLMKPKIELDRPPEVFKQVVSQQNAQRMRQI
                     MGLVTGGPSGTARGVFGPVKAAGINTGGKTGTAQKVVPVYDPKTGQPKTRHRVEKDNR
                     GNIIREYEETIMDEEHPRIDGWFLCIAPLERPQLAMAVIVEGGGYGSRSAAPIAAALV
                     LKAKDLGYFKTATAPKPATTNQRQVGVSPGRRRTN"
     gene            616..>2247
                     /locus_tag="VFV61_10600"
     CDS             616..>2247
                     /locus_tag="VFV61_10600"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEX4900973.1"
                     /translation="MKNRASSHLLVLLLIVGFEIAGYAAIHRAALLRGYETSIIGAVR
                     DLMMFFPLFVLLIWLSRVMKFKGNWILFTSAILLFAFGMMIQYRLYSDPEYNARNKAA
                     AREEKMEALRTRYILENYAPDKKQMMGLPPTPARPIEIDQLPQRPSTYTIFNALTSSY
                     TWIPILSFIAFAIAYSFCVRDGFLLWIQRNSFIIVLLTLLPLAAAIITSSAGKSLGNM
                     TPWEPAKIPFLLGFAGILTARYKDLAETYWGIPRARDIVPLVVMALIPFIPFFALKDF
                     GQMLIFSGAYATLYLVAVRRWPQLLIFVGSVFLVIGILVVGALPRDIQEKVPLLPTLA
                     RPIQATLPSRIQQRFHLWLDGFDPPSPEVSWWKKDYDEALNRTPELKEIAQQSPELQK
                     TVNVDIWFDKLAFQPAQAVFGITSGKTTGRGLGLGFPEVIPIADSDYVYAAIAEETGL
                     FGGAIIILAVIIFVAAGVRTSLDARDMFTKLCAAGLTAFVGFQALVNIGGILRALPMT
                     GITLPFVSHGGFSLITSYAMLGMLMAFSHRNAGDRL"
BASE COUNT          473 a          630 c          616 g          528 t
ORIGIN      
        1 atggcgctgg ctgccgcagc aattgggaac atggaaggca agttgatgaa gccgaagata
       61 gaacttgacc gacccccgga agtcttcaaa caagtcgtgt cacaacaaaa cgcgcaacgc
      121 atgcgacaaa tcatgggact cgtcactggc ggccccagcg gcacggctcg cggcgtgttc
      181 ggtccggtta aggcagccgg catcaacacc ggtggcaaga cgggaacagc gcaaaaggta
      241 gtacctgttt acgatcccaa gacgggacaa cctaagaccc ggcaccgcgt agaaaaggac
      301 aatcgcggca acatcattcg cgagtacgaa gaaacgataa tggatgagga acaccctcga
      361 attgacggct ggtttctctg catagcgccg ctagagcgac cgcagttggc gatggcggtg
      421 attgtcgagg gcggaggtta tggttcgcgg tctgccgcgc caattgccgc ggctctcgtg
      481 cttaaagcaa aggatctcgg atacttcaaa acagcaaccg cgcccaagcc ggcaaccaca
      541 aatcagcggc aggtgggagt gtcgccggga agaagacgaa ccaattgagg actgaatcct
      601 gactcctaac ttctgatgaa aaaccgcgct tcatcacatt tgctggtcct gctgctgatt
      661 gtcggctttg agattgccgg ctacgccgca attcatcgcg cggcattatt gcgaggttac
      721 gagacttcga tcatcggcgc cgtgcgcgac ctgatgatgt tcttcccctt gtttgtcctg
      781 ctcatctggt tatcacgcgt catgaaattc aagggcaact ggattctctt tacttccgcg
      841 atcctgctgt ttgcttttgg catgatgatc cagtaccgct tgtacagtga tccggaatac
      901 aacgcgcgca acaaggccgc cgcgcgcgag gaaaagatgg aggcgctgcg cacccgttac
      961 attctcgaga actatgctcc tgacaagaag cagatgatgg gcctgccgcc gactccggct
     1021 cgtcccattg agattgatca attgccgcaa cgcccctcga cttacactat cttcaacgcc
     1081 ttaacctcca gctatacctg gattccgatt ctttcgttca tcgcgtttgc gattgcctat
     1141 tcgttctgcg tgcgtgacgg cttcctactc tggatccagc gaaacagctt catcatcgta
     1201 ttgctgacgc tgcttccttt agctgcagcg atcatcactt ccagcgccgg caagagcctg
     1261 ggaaatatga ctccctggga gcccgcgaag attccttttc tgctcgggtt tgcgggaatt
     1321 ctgaccgcgc gctacaaaga cctggccgaa acttactggg gcattccgcg cgcgcgcgac
     1381 atagtgcccc tcgtggtgat ggctctaatt cccttcatcc ctttcttcgc gctcaaagac
     1441 ttcggacaga tgctgatctt cagcggtgcc tacgccacgt tgtacctggt ggcggtccgc
     1501 cgctggccgc agctactgat attcgtcggt tcggtgtttc tggtgatcgg gattctggtt
     1561 gtcggggccc tgccgcgaga catacaagag aaggtcccgt tgttacctac gctcgcccga
     1621 ccaattcagg cgaccctccc atcgcgaatt cagcaacgct ttcatttgtg gcttgatggt
     1681 ttcgatcccc catctccgga agtttcctgg tggaagaaag actatgacga ggcgctgaat
     1741 cgtactccgg agctgaagga gattgcccag caaagtcccg agcttcaaaa gactgtcaat
     1801 gtcgacatct ggttcgataa gctggcgttc caacccgcgc aggccgtctt tggtatcact
     1861 tcggggaaaa caaccggacg tggtttgggt ctcggctttc ccgaagtgat tcccatcgcc
     1921 gactccgact atgtgtatgc ggccatcgcg gaggagaccg gtctgttcgg gggcgcgatc
     1981 attattctgg cagtaattat cttcgtcgct gcgggcgttc gcacttcgct cgatgcgcgt
     2041 gatatgttca cgaaactctg tgcggcaggt ttaacggcat tcgtgggttt tcaagccctg
     2101 gtaaatattg gcggcattct gcgagcgctg ccaatgaccg gtattaccct tccgttcgtc
     2161 agccatggcg ggtttagttt gattaccagc tatgcgatgc taggcatgct tatggctttc
     2221 tctcaccgca acgcagggga caggttg
//
LOCUS       DASUEX010000637         4312 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1367862, whole genome shotgun sequence.
ACCESSION   DASUEX010000637 DASUEX010000000
VERSION     DASUEX010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4312
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1367862"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..582)
                     /locus_tag="VFV61_10605"
     CDS             complement(64..582)
                     /locus_tag="VFV61_10605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900974.1"
                     /translation="MSELDEAWAFALAEAETRARMAGHVDLAAYLSLRNANDLLRSTG
                     IQWLMSTCESLAGEANRRGAGIQIARDDKHRFRDENATMVGRLLTLTSGVRQLLIEAG
                     WPRVPRDGVVRGGGLARANIRHLGIKSANEALQLVTTKGVPRWVSLDASGPAKGIYES
                     ALRRHIDILLGS"
     gene            883..4188
                     /locus_tag="VFV61_10610"
     CDS             883..4188
                     /locus_tag="VFV61_10610"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEX4900975.1"
                     /translation="MKISGKRNTLPLLFIALALTFVGLSQFSVSSRAQDQVRLPARSG
                     YVNDFAGVVDESTEQRLEVVLGNLKQRSGIEFGVATIQTTGARDIFDVSRDLANEWNL
                     GARNSKKKSLLLVIAVNEKTAFTQFSRSVQLDLPEGILGEVSQIMRGSLASGRFGSGL
                     NDGVTHFIDSLARKAGFPAQDIDQVQVATAVAAEPLPAPTPAAEPVETVVKPAIDEKL
                     ALSTRPLRVNKPKESAPPAKAKSSPYINTAEDDADESEEVELVLTLPLAERVDKLKEF
                     LEAFPNSKSRPRAIELLISTHAALGDQNLKDGDRATGVKELSLAISEAPTDISDKLFA
                     GVVAQIPSNLYLRDEREAAFAAARAIEAKFGTDARRLLVLSSFYLGVEDGEEATRLAR
                     QAVKLAPEMAEAHRALGLGLHISLRLDEAADEYKRALELDPKTKQATRRSLADLQRAS
                     GKTEAALALYREQLALEPNDRAARAGVVLSLLELGRTEEGQKELDTALANEPQDLILL
                     AGAAYWFAAHDNSERALELAERAVRLEPRYTWSQIAMARALSGQKRPLEAERAIRFAG
                     QYGKFPTLDYELASVLASIGLYDEAADVLAHSFTYSRGKVATNLAGRLPMTADSFTEL
                     LAAERRASIFQFTGAERPGDAAMLKALLAFVAATEPGERARLDEDKAAAAAKEFASGT
                     DKMLAFRQMYAANRLLRRGVALPTALELTEAARASVDAALEVPGVTVAVQAEELRDMR
                     ARALAQGGTPDVPEAPRNVLANIMRGRVEDLTGLTLFKQDKTALSIDHFKRAVAILPE
                     GTPAWRNALWHLGTALDQEQQQQEALSYYIRSFNAGDPDPVRRSVIEELYRKMNGSLA
                     GLEERIGADLARRVTPATGVDTPASSAATPQSETVATPEPTPAPTTISELPASRPVEP
                     VAEATPTPEPVAVVPTPEPTPEETPAATPTASPEASPVTTPVVEPTPEATPEATPSPE
                     PTPSEERPTPTPEPARQPSDPLANPLPPAGEMRPPIASTVKLTGRIKNAAGEGIANVV
                     VVLISPRGTVLSSTTDVEGNYSFIVTPSPLGYRIIPSKEGFTFAPLDKVLSGISEDQN
                     AVDFVGTPEPAKP"
BASE COUNT          982 a         1203 c         1260 g          867 t
ORIGIN      
        1 cgtgtggttc gaaaacgtgc agagggaacg gaaatggtga ggggggtatg ccaatcggga
       61 ctatcacgat ccgagaagaa tatcgatgtg gcgtcgcaat gcggactcat agattccttt
      121 agccgggccg gaagcatcca gcgaaaccca gcgcggcact cccttggtcg tcaccagctg
      181 caacgcttcg ttggcagact tgatcccgag atgacggatg ttggcgcgcg cgagtccacc
      241 accgcgcact acaccatcgc gcggtacccg cggccaaccg gcttcgatga ggagttgtct
      301 gacgcccgaa gtaagcgtta agaggcggcc aaccatggtc gcgttttcgt cgcgaaagcg
      361 gtgcttgtcg tctcttgcga tctgaatgcc agcgccgcgc cgatttgctt ccccagcgag
      421 actttcgcaa gtgctcatta accactgaat gccggttgag cgtagcaggt cattggcatt
      481 gcgcagggaa agataggcgg cgagatcgac atgacctgcc atgcgggcgc gcgtttcagc
      541 ttccgccagg gcgaaagccc atgcttcgtc gagttcgctc acttgaatcg gttaacggtc
      601 atcggttatg gcgctactat agcgagtaat gacgggataa cgataagtcg gaaccgagtc
      661 tcgccagaga agcgagcccg atttcgtgga tcgcctttcc gcgcagtctt gaagctggca
      721 atccactacc ggaaatgaaa caacaccaaa taagattcgt ttgattcgcg gccttccagg
      781 tcgaatgtta tcatgccacc tgttggcttc ccccatttgt ctaatgaaat tccccgcttg
      841 tggtctgact taactaaaat agatcgtcct gagaactaat gcatgaagat atccggtaaa
      901 cgaaacacct tgccactcct gtttatcgcc ctggcgctta ccttcgtcgg tctaagtcag
      961 ttcagcgtca gctcgcgcgc gcaggatcaa gtgcgcttgc cggctcgttc cggttacgtc
     1021 aacgacttcg ctggcgtggt agatgagagt acggagcaac gactcgaagt ggtcctggga
     1081 aacctcaaac agaggagtgg aatcgagttt ggtgtggcca cgattcaaac gaccggcgcc
     1141 cgggatatct tcgatgtgtc tcgagacctt gccaacgaat ggaacttagg cgcccgcaac
     1201 agcaaaaaga agagtttgtt actggtcata gccgtcaacg agaaaacggc atttacccaa
     1261 ttcagccgct cagtgcagct ggaccttccg gaaggaattc ttggcgaggt aagccagatc
     1321 atgcgtggct cgcttgcttc cggtcgtttc ggttcggggc tgaatgatgg cgtgactcac
     1381 ttcatcgatt cgttagccag gaaggctgga tttcccgcgc aggatatcga tcaagttcaa
     1441 gtggcgactg cggtggctgc cgagccgttg cctgctccga ctccagcggc ggagccggta
     1501 gagactgtgg ttaagccggc cattgacgaa aaactcgccc tgagcacacg gccactcaga
     1561 gttaacaaac ccaaagagag cgcaccaccg gcaaaggcga agtcatcgcc ctacatcaac
     1621 actgctgagg acgacgcgga tgaatccgaa gaggtggaac tggtgttgac gttgcctctc
     1681 gccgaacgcg tggacaagct caaggagttt ttggaagcct ttcccaactc caaatcgcga
     1741 ccgcgcgcga tcgagttgct tatcagcacg cacgccgccc tcggtgatca aaacctaaag
     1801 gacggtgatc gagccacggg ggtgaaagag ttgtccctgg caatcagcga agcgcccacc
     1861 gacatttctg ataagttgtt tgcgggtgtg gtggcgcaga ttccttcgaa cctctaccta
     1921 agagatgagc gagaggccgc gtttgctgcc gcccgggcga tcgaagcgaa gtttggaaca
     1981 gacgcgaggc gtttgctggt gctctcgtcg ttctatctgg gagtggagga cggagaagaa
     2041 gcgacacggt tggcgcggca ggcggtcaaa ctagcacccg aaatggcaga agctcatcgc
     2101 gcgttgggat tagggttaca catctcattg cgcctcgatg aagctgctga cgaatacaaa
     2161 cgtgcgctcg agctggatcc taaaaccaaa caagcaacgc gccgcagcct ggccgacttg
     2221 cagcgcgctt ccgggaaaac ggaagcggcc ctcgcactgt atcgtgaaca acttgcgctc
     2281 gaacccaatg accgggccgc tcgcgctggc gtggtgcttt cgctgctgga acttggccgc
     2341 accgaggaag gacaaaaaga gctcgacacc gcgctggcga atgagccgca ggacctgatt
     2401 ctgcttgccg gagccgctta ctggtttgcg gcccatgaca actccgagcg cgccctggag
     2461 ctggcggagc gcgccgtgcg attagagccg cgctatacgt ggtcgcagat cgcaatggcc
     2521 cgcgcgctgt ccggacagaa acgacctttg gaggctgaac gagcgatccg ttttgccgga
     2581 cagtacggaa agtttcctac tctcgactac gagctcgcga gtgttcttgc ttctatcgga
     2641 ctctacgacg aagcggcaga cgtcctggcc cattctttta cttatagccg gggcaaggtc
     2701 gcaactaatc tcgccggtcg tttgcccatg actgccgaca gtttcactga actattggct
     2761 gcggaacgtc gcgccagtat ttttcagttt actggggccg aacgaccagg tgatgctgcg
     2821 atgttgaaag cactgctcgc ttttgttgcc gcgactgagc ctggcgagcg agccaggttg
     2881 gacgaggata aggctgcggc cgcggccaag gaatttgctt ccggcactga caaaatgttg
     2941 gccttcagac agatgtatgc ggcgaatcgg ctactgcgtc gtggcgtcgc gctaccgaca
     3001 gcgcttgaac ttaccgaagc ggcgcgcgct tcggttgatg cggcgctgga agttccgggc
     3061 gtcaccgtgg cagtccaggc agaagagttg cgcgacatga gggcgcgcgc tttagcgcag
     3121 ggtggcacgc ccgatgttcc agaagcgccg cgcaatgtgc tcgcgaacat catgcgcggc
     3181 cgggtagagg atttaacagg gctgactttg ttcaagcaag acaagacagc cttgtccata
     3241 gatcatttca aacgcgcggt agcaattctt cctgagggta cgccagcgtg gcgcaacgct
     3301 ctctggcatc ttggaaccgc actcgatcag gaacagcaac aacaggaagc gctcagctat
     3361 tacatccgta gctttaacgc tggcgatcct gaccccgtga gacgaagcgt gattgaagag
     3421 ttgtaccgga agatgaacgg ttcgttggca ggcctcgaag agcggatcgg cgcagacctg
     3481 gcgagaaggg tgacgcccgc aacgggcgtt gacactccgg cgtcctcggc cgcaacccca
     3541 caatccgaaa ccgttgccac accagaaccc acacccgcgc caacaacaat atcagagctg
     3601 ccagcgtcga gacctgttga gccggtggcg gaagcgactc ctacgcccga gccagtagca
     3661 gtagtgccga ccccggaacc gacgcctgaa gaaactcccg cagcgacgcc tacggccagc
     3721 cctgaagcat cgccggttac tactccagtg gttgaaccca cacccgaggc cacaccggag
     3781 gccacgccca gtcccgaacc gactcccagt gaagaacgtc caacaccaac gccggagccg
     3841 gcgcggcaac cttcagaccc actcgctaat cccttaccac ccgcaggtga aatgcgaccg
     3901 ccaattgctt ctacagttaa actgactgga agaattaaga atgcggccgg ggaaggaatt
     3961 gcgaacgtag ttgtggtctt gattagtccg cgcgggacag tactgagttc aacaactgac
     4021 gttgaaggga actactcgtt tatcgtgact ccctcaccac tcggttatcg gatcattcct
     4081 tcaaaagagg gtttcacttt tgcgcctttg gataaggtgc tgagcgggat ttccgaggat
     4141 cagaatgcag tggattttgt aggcacgccg gagccggcga aaccgtgaat cgtgaatcgt
     4201 gaatgtgaat cgtaaatcgt gaatggtaaa tcgtgaatcg cgtgctagct aatattcata
     4261 accgccaagc aactgtgtcg taagtcaagg ggatcaggca gtcctccaag at
//
LOCUS       DASUEX010000638         2395 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1253318, whole genome shotgun sequence.
ACCESSION   DASUEX010000638 DASUEX010000000
VERSION     DASUEX010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1253318"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..822
                     /locus_tag="VFV61_10615"
     CDS             <1..822
                     /locus_tag="VFV61_10615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CofH family radical SAM protein"
                     /protein_id="HEX4900976.1"
                     /translation="AGEAVAEGATELHIVGGLNTKLPFSYYTDLLSSLKRQYPQLHLK
                     AFTMVELDHFAHFYKMGDDEVIQKLIEAGMDSCPGGGAEIFREPTRSMICAHKCDADR
                     WLELSGKVHASGLKTNATMLYGHIESIEDRVDHFVRLREQQDKSQGFQCFIPLAFYPP
                     GTQLAHLPGPSGVDSLKTMAVARLLLDNFDHIKAYWVMLGRRLAQVALHYGANDIDGT
                     ITKGGELSESYSVEINNEVKMSKADLVALIEDAGFTAVERDTVYNRVTPSNVSLV"
     gene            867..1715
                     /locus_tag="VFV61_10620"
     CDS             867..1715
                     /locus_tag="VFV61_10620"
                     /inference="COORDINATES: protein motif:HMM:NF014658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MqnA/MqnD/SBP family protein"
                     /protein_id="HEX4900977.1"
                     /translation="MQPKKAVRKISVAHSPDSDDAFMFYGLATSKLDTGTINFTHVLK
                     DIQTLNEEAMRGTYDVTAVSFHVYAYIADKYALLPHGASIGDKYGPIVVSHEPVKADE
                     LANLKIAVPGTLTSAFLALKIFNPDFKYEVVPFDKIIETVQSRRCDAGLLIHEGQLFY
                     ESIGLHKVLDLGEWWHEKTGLPLPMGGNVIRRDLGSATITEVSRLLRESIRYSLDNRD
                     DALQYAMQFARDMDLELADRFVAMWVNDLTLDYTERGRTAVQRLLDEGFDHGIIPNRV
                     AVEFAE"
BASE COUNT          557 a          606 c          648 g          584 t
ORIGIN      
        1 gccggtgaag cggttgccga aggtgcaacg gaactacaca tcgttggtgg cttgaatacg
       61 aaattgccgt ttagttacta caccgatttg ttgtcgtccc tgaagcgcca gtacccccaa
      121 cttcatttga aagccttcac aatggtggag ctcgatcatt ttgctcattt ctacaaaatg
      181 ggcgatgacg aagtgattca aaagctcatc gaagccggca tggactcgtg tcctggcggt
      241 ggcgccgaga tttttcgcga accgactcgt tcaatgattt gcgcccataa atgcgatgca
      301 gatcgctggc ttgaactctc aggaaaggtg catgcgtccg gtttgaagac aaacgccacc
      361 atgctttacg ggcacatcga gtcgatcgag gatcgcgtcg accattttgt gcgtctacgc
      421 gagcagcagg acaagtcaca ggggtttcag tgctttattc ccctggcttt ctatcccccg
      481 ggaacgcaac tggcgcattt gcccggtcct tcgggagtcg attcgctcaa gaccatggcg
      541 gtcgcgcgct tgctgctcga caacttcgat cacatcaagg cttattgggt gatgttgggc
      601 cggcgccttg cccaggtcgc cctgcactac ggtgctaatg acatcgacgg aacgatcacg
      661 aaaggcgggg agctttcgga aagctactca gtggaaatca acaatgaagt gaaaatgtcg
      721 aaagcggatc ttgtggcgct gattgaagac gcaggtttta ccgccgttga gcgcgatacg
      781 gtttacaatc gggtgacccc gagtaacgtc agcctggtct gaatcttccc gacctctaat
      841 catctaacta aggaattaca agatttatgc aaccgaaaaa ggctgttcga aaaattagtg
      901 tggcgcattc tcccgactcg gatgacgctt ttatgtttta tgggctggcc acaagcaagc
      961 tcgataccgg caccatcaat ttcacgcacg tgcttaaaga cattcaaact ctaaatgaag
     1021 aagcaatgcg gggaacttac gatgtaaccg cggtgagctt ccatgtctac gcgtacatcg
     1081 cggacaaata cgctcttttg cctcatggag cctcgatcgg agataagtac ggtccgatcg
     1141 tcgtctcaca tgaacctgtc aaagctgacg aattggcaaa tctgaagatt gcggttccag
     1201 gcacgctcac cagcgcgttt ctggctctta agatcttcaa tcctgacttt aaatatgaag
     1261 tggtgccgtt cgataagatc atcgagaccg ttcaaagcag acggtgcgat gccgggttgt
     1321 tgattcacga aggacaactc ttctacgaat caatcggact ccacaaggtg ctggacctgg
     1381 gcgaatggtg gcatgagaaa accggattgc cattgccaat gggtggcaat gtgattcggc
     1441 gtgatttggg ttcggcaaca atcacggaag tttctcgcct tctgcgcgaa agtatccgtt
     1501 actcgctcga caatcgcgat gatgctctgc aatacgccat gcagtttgcc agagacatgg
     1561 atctcgaact cgcggatcgg ttcgtcgcga tgtgggtcaa cgatctgacc cttgattaca
     1621 ctgagcgcgg ccgcactgcc gtgcagcgtt tgctggacga gggttttgac cacggaatca
     1681 ttcccaaccg ggtcgcggta gaatttgcgg agtagtggtt agaagtgcga atgctggggt
     1741 gtgcgtgacg acctctcttc ccgctccgcc cagaaggagc gggaaagtcg ccacgcacat
     1801 cgtcgacctc tggcggtatc aagctcctcc cgaaacttga attacgcccc tcccgaatgc
     1861 gaacccgggt gccggtttgc cactacgctt gcgcgccgta tcggttcacc ggaattagtg
     1921 acgtagcctt ctgccgacgt gaggacttaa gatttcgtct ttatcctcaa ggctatccct
     1981 ttcgctgagg atgtccgcca atgattctat tgacgcccac tggacacggc tgcggataac
     2041 ctattcaatg gctggcactt tacactagca gcaaggtttt ttgcaaacaa aaataattac
     2101 agtcaagtcc tgcgctagtg gtagctcgga tattggcaac agtggcggcg gtgcgggcat
     2161 ctgaggttgg tgcccggcag gtcacggatc agtgttttaa gttggcattc atggtgtttt
     2221 tggattggtc atactccagg ttctggaatt agaaatgcga acgacgaatt ttcgaagagt
     2281 agcggttctg gtaatggcgc tgtccatctc actcttggcg gcgccgcttg ctcttgcgca
     2341 gcaaaccaat ggaagcgagc caagtcaggc ggaaattaac aggattattg cagcc
//
LOCUS       DASUEX010000639         4381 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_798074, whole genome shotgun sequence.
ACCESSION   DASUEX010000639 DASUEX010000000
VERSION     DASUEX010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4381
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_798074"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(301..1221)
                     /locus_tag="VFV61_10625"
     CDS             complement(301..1221)
                     /locus_tag="VFV61_10625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HEX4900978.1"
                     /translation="MKLNSRIVIAIVVNLLLAALASAQQRPDPNFNISVEQPAYTKNY
                     PRVLFDEAHNNFHTTTGRYKPFADLINNDGYHVVVNRKPFTRPALDTFKVLVIANALG
                     AEEMDDEGADKPAFTEEECDAVRDWVKAGGALLLIADHAPFGGAAENLGKRFQIDMSK
                     GYTLDAANSFVPDAPSSLLFSRENKLLLDHPITRGRNDSELIKRVYTFTGQSLKGPKD
                     SFAFMILSDTAQDKADRESATLISAAGRAQGIALKFGKGRVVVMGEAAMLSAQLAGSQ
                     ERPMGMNVPGSDNRQLALNIMHWLSGILGK"
     gene            complement(1234..2433)
                     /locus_tag="VFV61_10630"
     CDS             complement(1234..2433)
                     /locus_tag="VFV61_10630"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX4900979.1"
                     /translation="MTTPAPSQHRHVSHKASLFTESVIREMTREALKHGAVNLSQGFP
                     DFAAPADIKQVAMQAIADDINQYAITWGARDFREAIAKKTRWYLGLEVDPETELTVTC
                     GSTEGMIAAMMATVDPGDEVVIFEPFYENYAPDAILSDARPRYVPLRAPDWTFDPDEL
                     RAAFNNKTKAIIICNPNNPTGKVFTRAEMEFIAGLCQEFDALCFTDEIYEHIIYPRSG
                     AEIQHISMAQLDGMRERTVIVNSMSKTYSVTGWRVGYCIAAPEITSGIRKVHDFLTVG
                     AAAPLQAAGAYALSLPPQYYERLQAEYRARRDLLLPVLDEAGFKTFRPDGAYYIMTDI
                     AAFGFADDVEFTRHLIREVGVACVPGSSFYSVPERGSQQVRFCYCKKDETLALAAERL
                     EKLRARI"
     gene            complement(2857..3798)
                     /locus_tag="VFV61_10635"
     CDS             complement(2857..3798)
                     /locus_tag="VFV61_10635"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX4900980.1"
                     /translation="MKAHRGLSAVRKAGLPSLFSACVSTLSLVLLSTTSAQRTPAPAL
                     TITTEPNAIVWLDEIRRGTTDATGKLVLTKISPGRHNLRVRANGFREVTVPLPAGRRT
                     AAVKLVRTTDAAEIAFQRAETAREQAKTEAARGQAVELYREALRLRPAYPAAHVGLAR
                     VLLELNEYPDALAEIEAARRNRPVYAEASAVEGRIHRESAFDEKAIASFRRAIREGKG
                     VQPEAHVGLARVLEEKGQLDEAVAEYQRALAQLSDSEPVIYQLLGAAYEKQQKYKEAL
                     AAYEKYLALAPNGSLAPALRSIIEQLRRDTAGQEIIP"
     gene            complement(3907..>4381)
                     /locus_tag="VFV61_10640"
     CDS             complement(3907..>4381)
                     /locus_tag="VFV61_10640"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEX4900981.1"
                     /translation="RAKSAARAYGLMQVLVPTATLTAKKYGVDRSITVDSLFEPRLNI
                     QLGTAYLRDQIDKFGRIEYVAAAYNAGPGRAVSWRTSLPLEIDEWAEAVPFKETRGYV
                     QGVVRNRLQYERLYDINGNFRPEVGTKAVTAQPSGSPPADSTIRKRRVIGDEEEE"
BASE COUNT          921 a         1223 c         1223 g         1014 t
ORIGIN      
        1 ggttttgagc ccgctcttcc cgttgcggcg agccgcgctg ccttgccatc gccgcaggac
       61 gttcccttaa ccgactatcc gcaacgaatc aacacccggg aaatggttcc tcgcaacagc
      121 gtaaccgaaa acacaacgcg gttgttggat gaagaatgac tctggtaaca aggttcaact
      181 caagtacaga acgggagcgg cagcgaccga taagattctc aattttcaat tgccaatttt
      241 tgattggacc tttggctaag gactaatcgg caatcgccat ttgttctggc ggtgtttcag
      301 ttacttacct aatatacctg acagccaatg catgatgttc aaggcgagct ggcgattatc
      361 gctgcccgga acgttcatcc ccatcggccg ttcctgcgat cctgccaatt gtgctgacag
      421 cattgcggct tcacccatca ccaccactcg acccttgcca aacttcaaag caataccctg
      481 cgcgcgtcct gccgccgaga tgagagtagc cgattctctg tcagccttgt cctgcgctgt
      541 gtcgctgaga atcatgaaag caaagctgtc tttgggtcct ttgagtgatt gacctgtgaa
      601 ggtgtatacg cgtttgatca actcgctgtc gtttcgaccg cgcgtaatcg gatggtcgag
      661 taagagcttg ttttcccggg agaagagcaa cgaggaagga gcgtccggca caaagctatt
      721 tgcggcgtca agtgtatagc ccttgctcat atcgatctga aagcgcttac ccaggttttc
      781 ggcggcgccg ccaaacggag cgtgatccgc aatcaagagc agcgcgcctc cggcctttac
      841 ccaatcgcga accgcatcgc attcttcctc agtaaaagcc ggtttgtcag cgccctcatc
      901 atccatttcc tccgccccaa gagcgttggc gatcaccagc accttgaacg tgtccagcgc
      961 aggtcgagtg aatggcttgc gattgaccac cacgtgatag ccgtcgttgt tgatcagatc
     1021 ggcaaatggc ttgtagcgcc cggtcgtcgt gtgaaaattg ttgtgcgctt cgtcgaaaag
     1081 cacgcgggga tagtttttgg tgtaagccgg ttgctcaaca gagatattga aattgggatc
     1141 gggacgttgt tgtgcgctgg ccaaagccgc aaggaggagg ttaacgacga tagcaatgac
     1201 aattcgcgag ttcagtttca tggaaggtcc ttatcaaatg cgagcgcgca gcttttccaa
     1261 acgctccgct gccagcgcaa gcgtttcgtc ctttttgcaa tagcagaatc taacctgttg
     1321 cgagccgcgc tccggaacgc tgtagaagct tgagcccggc acgcaggcga ctccaacttc
     1381 ccggatcagg tggcgggtaa actccacgtc atcggcaaat ccgaaagccg caatgtcggt
     1441 catgatgtaa taagcaccgt ccggtcgaaa cgttttgaag ccagcctcgt ccaacaccgg
     1501 taaaagcaaa tcgcgacgcg cgcgatattc agcctgcaag cgttcgtagt attggggcgg
     1561 aagtgatagt gcgtaggcgc cagctgcttg taagggagcc gcggcgccga cggttagaaa
     1621 gtcgtgtacc ttgcggatac cgctcgtgat ctccggagcg gcgatgcaat agccgacgcg
     1681 ccagccggtg accgaatagg tcttggacat ggaattgacg atcacagtgc gctctcgcat
     1741 gccgtcaagt tgcgccatgg aaatgtgttg aatctccgcc ccgctgcgcg ggtagatgat
     1801 gtgttcgtag atttcatcgg tgaagcagag agcatcaaac tcctggcaga gacctgcgat
     1861 gaactccatt tctgcacgtg tgaaaacttt gccagtcgga ttgttgggat tgcagatgat
     1921 gatcgctttt gtcttgttgt tgaaggctgc gcgcaactcg tctggatcga aggtccagtc
     1981 cggagcgcgc aagggcacat agcgcgggcg cgcatcggag agaatcgcat cgggtgcgta
     2041 gttttcgtag aacggttcaa agatcacgac ctcatcaccc ggatcgacgg tggccatcat
     2101 cgcggcaatc attccttcag tcgaaccgca ggtgaccgtc aactctgttt ccggatctac
     2161 ctctagtccc agataccacc ttgtcttctt tgcgatcgct tcgcgaaaat cgcgcgcgcc
     2221 ccaggtgatt gcatattgat tgatgtcgtc ggcgattgcc tgcatcgcga cttgtttaat
     2281 atccgccggg gcggcgaaat cgggaaagcc ttgcgaaaga tttaccgcgc catgcttcag
     2341 agcttcgcgc gtcatttcgc gaatcaccga ttcggtaaac aggctggctt tatgggaaac
     2401 gtgccggtgt tgggaagggg caggtgtagt catggagatg aagtacgcgg ggtagtcagc
     2461 gctcttaatg atttccagtt gaacgaagcg atgctcgcat agaagtcctg agtgttcaag
     2521 aaaacagcag cgcggagtcg atgtatggtt tcccgcgaga cactagcgcc gaatagatgc
     2581 cggtgcgcga taagcaacga tgaattcatc cggtcccgtc tcccccgcgc cgcgtagcgg
     2641 cccctgactt tagcccggct tttcaaagcc gggttggcca caaaagcatc gttcggcgtc
     2701 gcgtgagcga cgctgactaa aaagttttat ttcaatcgtc gctacgcgac gaactcaagt
     2761 atgggacctc ttaccgggcg ttgaaacgcc cggctaaagt ctttcgacgc taccgcgtcg
     2821 aagctgttga gcgaatttcc ttgacgctac gcggccctaa ggaatgattt cctggcccgc
     2881 ggtgtcgcgg cgcaattgtt cgatgatgga tcgaagtgcc ggagcaagac tgccattagg
     2941 agctaacgcg agatacttct cataagccgc gagtgcttcc ttgtacttct gttgtttctc
     3001 gtaagcggct cccaggagtt gatagatcac cggctctgaa tcggagagtt gggccaacgc
     3061 cctttgatat tcagcaaccg cctcgtccag ttggcccttt tcttcgagca cgcgtgccaa
     3121 gcccacatga gcttccggct gtacaccctt gccttcgcgg atcgcgcggc gaaaagaagc
     3181 aatcgctttt tcgtcaaaag cgctttcgcg atggatgcgt ccctcaactg ccgacgcctc
     3241 cgcgtaaact ggacgattgc gacgagcagc ttcgatctca gcgagagcgt ccggatattc
     3301 gtttaactcc agcagcaccc gagccagtcc cacgtgcgcg gctggatatg ccggccgcag
     3361 tcgcagggct tcacgataga gttccaccgc ttgccctcgc gctgcctcag tcttcgcttg
     3421 ttctcgggcg gtttcggcgc gttggaaggc aatctcagct gcatcagtag tgcgcaccag
     3481 cttaacggcg gctgtgcgtc ttcccgccgg taatggcacg gttacttccc taaaaccgtt
     3541 ggcgcgcacg cgcaggttgt gccggccggg cgaaattttt gtcagtacca gtttgcccgt
     3601 cgcgtccgtg gtaccgcgcc tgatttcatc aagccagaca attgcgttgg gttccgtggt
     3661 gattgttagg gcgggggcag gagttcgttg tgccgaagtc gtgcttagca aaactagact
     3721 gagagtgctg acgcaagcag aaaagagcga gggcaagccc gccttccgga ctgcgcttaa
     3781 tccacgatgc gctttcataa tctcaccgaa tcgcttcccg tttcgtcaaa acaccgccaa
     3841 gcgccaaggt gcgaactcgg cggatcttgg cgtcctggcg gttttcttgc tggttaactt
     3901 ccgagtctat tcctcttcct cgtctccaat cacgcgtcgt ttgcggatcg tagaatcggc
     3961 tggcggcgat ccggagggct gcgcggtgac tgctttcgtt cccacctccg gccgaaagtt
     4021 gccattaatg tcgtagagcc gttcatactg aagacggttg cgtaccacgc cttgcacgta
     4081 accgcgcgtc tccttgaaag gcacggcttc ggcccactca tctatctcga gcggtagtga
     4141 agtgcgccaa ctgacagcgc gtcctggtcc ggcgttgtaa gccgcggcga catattcgat
     4201 gcgtccaaac ttatcgatct gatcacgcag gtaagccgtg cccagttgaa tgttcagccg
     4261 cggttcgaag agcgagtcaa ccgtgatgct gcgatcgacg ccatacttct tcgccgtcaa
     4321 tgtagcggtc ggcactaaca cctgcatcaa gccataggct cgagccgccg acttcgcgcg
     4381 c
//
LOCUS       DASUEX010000640         2318 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1025977, whole genome shotgun sequence.
ACCESSION   DASUEX010000640 DASUEX010000000
VERSION     DASUEX010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1025977"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(418..1854)
                     /locus_tag="VFV61_10645"
     CDS             complement(418..1854)
                     /locus_tag="VFV61_10645"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX4900982.1"
                     /translation="MLWQRHGTHMTKALLLLVLLGTFSPIVPTQEPARNVAKLQARTT
                     RDWVRDGVVYEIYPRAFSEAGNFNGITARLDHLKDLGVNILWLMPIHPIGQEKKKGTI
                     GSPYAVRDYYGINPDYGTKEDLKKLITEAHRRQMKVIIDIVANHTSWDNVMMKTPDFY
                     ERNAAGQITYPHDWSDVAELNYKNPQLRQYMIDMLKYWIREFDLDGFRCDVAEEVPTD
                     FWETARTELDKIKPDIVMLAEGHKAELLVKAFDFDYSWPIHSSLTRVLQGQSRASDLR
                     TEWEKEFKDWPRGSLHLRFSDNHDERRAIARFGEPAALAASALVFTLDGVPLLYNGME
                     VGDTTESGAPALFEKLPVFWSIAERRPEFPRFYKQMITLRKSNKALRQGALEWLPNSD
                     ESRVLTFVRRTPDEEVLVAINLSNRPFFGTTEAAHGAGYEDISPDVGPPLPPDAPAPE
                     RAARKRPVGLPALSLDAWGYRIFRRALK"
     gene            complement(2115..>2318)
                     /locus_tag="VFV61_10650"
     CDS             complement(2115..>2318)
                     /locus_tag="VFV61_10650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900983.1"
                     /translation="DNPNAPMYVVMAGGVFLALAAISVLLVQDVADKNVPEAAVIEAD
                     EHALLSVPESVQPVPSTGLIDEK"
BASE COUNT          486 a          662 c          632 g          538 t
ORIGIN      
        1 cattctttgc cgcgctgggc caatgcgcat cgagcccggg tgcgccggga gcgttcgtct
       61 gcccgaacac aacggtgcaa ccgaggagat ataggatcgt aattcggatg ggtcgcagca
      121 tttagcgtca gcctgaatta gtcgtggttc aagtccgcgg agcggacgga agcatagagc
      181 ctggggcgta agccccaggt aaagttccgg aatatgatcg agcccgtgaa acgggcgaaa
      241 gccccttgag tggttccatt cgctgtcgcc cgcttcgcgg gcttgtaaat cttcagcgct
      301 ccttgacctg gggcttacgc cccaggctct atgctttcac ccgcttcgca ggttggttga
      361 acttcgggtt ttgggcagag tcgatcccac gggcaaattt catggacagc cactctctca
      421 cttcaacgct cgtcgaaata ttcgatagcc ccaggcatcg agcgagagcg ccggcaaccc
      481 gactggtcgt tttcgagcag ctctctcagg cgcgggtgcg tccggtggca aaggtggacc
      541 gacatccggc gagatatctt cataccccgc gccgtgcgcg gcttcagtag tgccaaagaa
      601 gggacgattc gacaggttga tcgcgactag cacttcctca tcaggcgtgc ggcgcacgaa
      661 ggtcaacacc cgcgattcgt cagaatttgg gagccattcg agcgcaccct gccgcagcgc
      721 tttgttcgat ttgcgcaaag tgatcatctg tttgtaaaac cgcggaaact cgggtcgtct
      781 ttcagctatc gaccagaata ccggcagctt ctcaaagaga gcaggcgccc ctgactcggt
      841 cgtgtcgccc acttccattc cgttatacaa aagcggcaca ccatcaagtg tgaacaccaa
      901 tgcggaagcg gcgagtgcgg cgggttctcc gaatctggca attgccctcc gctcgtcgtg
      961 gttgtcagag aagcggagat gcagcgagcc ccgcggccag tctttaaatt ccttttccca
     1021 ctcagtgcgc aaatcggaag cgcgactttg tccctgcaac acgcgagtca gcgagctgtg
     1081 aattggccac gagtagtcaa agtcaaaggc cttaacgagc agttccgctt tatgaccttc
     1141 ggcgagcatg acaatgtcag gtttgatctt gtctaactcg gtccgagcgg tctcccaaaa
     1201 gtcagtgggc acttcctcag ccacgtcgca gcggaagccg tcgagatcaa actcgcgtat
     1261 ccagtacttc aacatgtcaa tcatgtactg gcggagctgg gggttcttgt aattaagttc
     1321 ggcgacgtcg ctccagtcgt gtgggtaggt gatctgtcct gcggcattgc gttcataaaa
     1381 gtccggggtc ttcatcatga cgttgtccca ggacgtgtgg tttgccacga tgtcaatgat
     1441 cactttcatc tggcgccggt gcgcttcggt aattaacttt ttcagatctt ccttggtgcc
     1501 gtagtcgggg ttgattccgt agtaatcgcg caccgcgtag ggactgccga tcgttccttt
     1561 tttcttttcc tgtccgatgg gatgaatcgg catcagccag agaatgttca ccccgagatc
     1621 tttgaggtga tccagtcgcg ccgtaattcc attgaagttg cccgcttcag aaaacgcacg
     1681 tggataaatc tcataaacga cgccgtcgcg tacccagtcg cgcgttgtgc gggcttgcag
     1741 cttcgcgaca ttgcgtgccg gttcttgagt ggggactatc ggagagaacg tcccaagcaa
     1801 aaccagaagg agaagagctt ttgtcatatg agtcccatgt cgctgccaga gcatagtctt
     1861 tcctctcact gctgttctac caagatagaa acggctttgc cgccggatgt gaggcgcggc
     1921 ttgccgcgcc gtgctcgacc tactgatttt gggctgcgcc cttcgttcgg acctactaca
     1981 atctgacgcc gcggcccaca attagggcgt agcccaaaat ttccagtccg aacaacggcg
     2041 cggctaagcc gcgcctcaca tccggcggca tagccgtaac tcttaggttg tgttcatgtc
     2101 ctctgtaaaa gctgtcactt ctcatcgatc aatcctgtac ttggcaccgg ttgcaccgac
     2161 tccggaacag aaagcagcgc atgctcatcc gcctcaataa ccgcagcttc cggcacattc
     2221 ttatcagcga cgtcttgcac caataagacg ctgatcgcgg caagcgccaa aaaaactcct
     2281 cccgccataa ctacgtacat cggtgcgtta ggattgtc
//
LOCUS       DASUEX010000641         3402 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_537947, whole genome shotgun sequence.
ACCESSION   DASUEX010000641 DASUEX010000000
VERSION     DASUEX010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3402
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_537947"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="VFV61_10655"
     CDS             complement(<1..204)
                     /locus_tag="VFV61_10655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900984.1"
                     /translation="MKRVFSGTLSAVILCALIATFATPAGASKGTLATIAGTVSDSKG
                     NPVSGALVSLLKEGAKEISKQMRT"
     gene            537..1805
                     /locus_tag="VFV61_10660"
     CDS             537..1805
                     /locus_tag="VFV61_10660"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX4900985.1"
                     /translation="MIDEVQSTKLTNGLVVLSEHMPGLRSVTAGIWVRRGSRHESPEL
                     NGICHFIEHSVFKGTKRRTAHDIAVESDRLGGHLDAYTTHEMTGFAIKVADQSLPAAL
                     DLLADVVANPKFSQEDLEREQSVILEEMKMVEDTPDELLGELFNAAYFPDQPLGRPIE
                     GTRETVSSFDCETTSLFHSREFAAANLVIAAAGKVDHAQLVDLVSASFEHRNGNSQLT
                     EVRPPAPAAPILIEQKKELEQAHLIIATPWSTARSDGRYAASLLASVIGGGTSSRLWQ
                     KIREERGLAYSVGAGGSTFSDVGVFTIYAGTSPEHMDEVVDLSLNELRRIVRESVSLE
                     ELQLAKDQAVSSILLGLESSSARAGTLARQEIIHGRRISPDEIISSIEAVTPDEIQAV
                     ARQFFTTDTLALGALGNLNGFRVDRSRLEI"
     gene            2041..2862
                     /locus_tag="VFV61_10665"
     CDS             2041..2862
                     /locus_tag="VFV61_10665"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX4900986.1"
                     /translation="MKLTVLGSGSTGNVILIVAGETRVLVDAGLSAKEISRRLALVGE
                     DVQRLDGILVTHEHGDHAGGLRVLLNQVDCPVYISAQTCDAYVSERSVGNEEPRKRAK
                     ALKGRVEHIESSRDFCIGEIDFHPFTIPHDAADNFGFTATHQGVKIATLMDFGHITTL
                     ITERLRGCAAIVIEANHSRDMLKACDAYPWDLKQRILSRLGHLSNEDVADWIKRDFDG
                     AAGFIVLAHLSQRANNPYLAKISAEVALQERSPLFHTQTQVSLSFPKEPTPWIEV"
     gene            2996..3349
                     /locus_tag="VFV61_10670"
     CDS             2996..3349
                     /locus_tag="VFV61_10670"
                     /inference="COORDINATES: protein motif:HMM:NF024609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4019 domain-containing protein"
                     /protein_id="HEX4900987.1"
                     /translation="MPPEIDALVSSISDDIAAERYQKVYDEAAELWRQDSTAEQSAAA
                     FKTLKEKLGNVKYRTVHSATEQQNSGGDLKGRVFIIGYQTTFEKGEGMETFTFVERDH
                     QWKLARYRINSMALR"
BASE COUNT          757 a          891 c          918 g          836 t
ORIGIN      
        1 agttctcatc tgttttgaaa tctcttttgc gccctctttg aggagtgaaa cgagcgcacc
       61 agacacagga ttgcctttgc tgtcacttac ggtaccggca atcgttgcga gagtcccctt
      121 gcttgccccg gcgggagtcg cgaaggtcgc gatcaaggcg cataagatca cagcactgag
      181 agtgccgctg aatactcttt tcattgcagc gaaacctcca agagcagggc tcctgacgga
      241 gcagttatat cggggaaggc atctgtctgg ttcgctaaag ccggaatgac tttcaggttg
      301 attcatttcc gggaaacaac ggttaatctg gtttctagtc cgaaagcgcg aactggttca
      361 tggcaggaga cttaccggaa ccctctgata gggtgtcatt ttccgtcaag gttttacagc
      421 ctgtcaagca attctgccct tgccgcaccg cctttgccgc cagtttggcc ccgtgctacg
      481 atgcttttcc ccacaaaacc aaggctcact gctgcaattt cgggccagga cgttccatga
      541 tagacgaagt tcaatcaacc aaacttacaa atggccttgt cgttttgtcc gagcacatgc
      601 cgggcttgag gtccgtgact gcgggaattt gggtgcgccg tggttcccgt cacgaatcgc
      661 ccgaacttaa tgggatttgc cactttattg agcattctgt tttcaaggga acaaaacgcc
      721 ggacggctca tgatattgct gtcgaatctg atcggctggg cggccatctg gatgcctaca
      781 ctacccatga gatgactgga ttcgccatta aggttgcgga ccaaagtctt cccgcggcgc
      841 ttgatttgtt agcggacgtt gtcgcgaatc caaaattctc ccaggaagac ctcgagcgag
      901 agcagagcgt gatcctcgaa gagatgaaaa tggttgagga tacgccagac gaactgctgg
      961 gggagctttt caacgctgct tacttcccgg atcagcccct tggtcgtccc attgagggta
     1021 cacgcgaaac cgtctcgtct tttgattgcg aaacgacttc gctctttcat tcgcgcgaat
     1081 ttgcagcagc taacctcgta atcgcagcgg ctggaaaagt cgatcacgcg caattggtgg
     1141 atctggtaag tgcttccttc gaacaccgaa acggcaattc ccagttgacg gaggttcgtc
     1201 caccggcacc cgctgcccca attctcatcg agcagaagaa agagcttgag caggcccact
     1261 tgattatcgc tacgccctgg tcaactgctc gcagcgatgg tcgatacgcc gccagtttgc
     1321 tggcatcggt tatcggcgga ggtacgtcaa gccggctttg gcagaagatc cgtgaggaac
     1381 gcggtcttgc ctactcggtc ggcgctggcg gaagcacctt ctcagacgtt ggagtcttta
     1441 ccatctatgc gggcacctct ccggaacaca tggatgaggt agtcgatttg tcgctgaatg
     1501 agttgcgccg catcgtccgg gagagtgttt ctttggaaga gttgcagctc gcaaaagacc
     1561 aggctgtttc gtctatcctg ctcgggcttg aatcgtcgag tgctcgtgcc ggcacgcttg
     1621 ctcgccagga gataattcac ggacgacgga tctcacctga tgagatcatt agcagcatcg
     1681 aagcagtcac gcccgatgag atacaggcgg tcgcgcgtca gttcttcact actgacactt
     1741 tggctttagg tgcgttaggc aaccttaacg gctttcgcgt cgatcgatcg cgcctggaga
     1801 tttaagtctt tccacttcgc gtccagttcc cgttttcatt cccacccgtt cttccttgta
     1861 gtctcaaacg tcccggttcc tacgccttcg ctcgtcgtga ggtctacgca gtagaagctc
     1921 aagctcaact acagtctacc ggggcaccac taaaaatatt tagatgtatg gccgtttctt
     1981 ttcttaagtg ttaaagtcct gttttcgttt cctgtctgat tagagccata ctgaaatcat
     2041 atgaaactta ccgttctggg cagcggttca accggcaatg tgatcctgat tgttgcaggc
     2101 gagacacgcg tcctcgttga cgctggtttg agtgcaaaag agatttcccg ccggctcgcc
     2161 ctggttggcg aagatgtgca gcgtctggac ggcatactcg tcactcatga gcatggtgat
     2221 cacgccggtg gcttgcgagt cctgcttaac caagttgact gtcctgtcta tatttcagcc
     2281 caaacttgcg atgcctacgt cagtgaacgc tccgtgggaa atgaagagcc gcggaaacga
     2341 gccaaggcgc tgaagggacg agtggagcac attgaatcaa gcagagactt ttgcatcggc
     2401 gagatcgatt ttcacccgtt tacaattccg cacgacgcag ccgacaactt tggctttacg
     2461 gctactcacc agggcgtgaa gattgccacg ctgatggatt ttggacacat tacaaccctc
     2521 atcacagagc ggttgcgcgg ttgcgcggcc atcgtcattg aggccaatca cagcagagat
     2581 atgctcaagg cttgcgacgc ttacccctgg gatttgaagc agagaattct ttcgcggctg
     2641 gggcaccttt caaacgaaga tgtggcggac tggataaagc gggactttga tggcgcggcc
     2701 gggtttatcg tgctggccca tctttcgcag cgggctaaca atccctactt ggctaagatc
     2761 tctgccgaag ttgcgttgca ggaacgctcg ccgctgtttc acacacaaac tcaggtcagc
     2821 ctttcatttc ccaaagaacc gacgccctgg atagaggttt gagtggatga tggaacatgg
     2881 ccgcacgatt cttctcccac tagcaccaac ccgtccttcc gttccaacca taatcctggt
     2941 gcttctcagc ttgtttttgg tgtctggctg cggtctaaaa aatactcgcc gtgaaatgcc
     3001 gccggaaata gacgcgctcg tctcttcgat cagcgatgac attgcggcgg agcgttacca
     3061 gaaggtctac gatgaggcgg cggaactctg gagacaggat tcaactgccg aacagtctgc
     3121 cgccgcattc aaaacgctga aagaaaaact gggtaacgtg aagtatcgta cggtccattc
     3181 tgcgaccgaa cagcagaatt cgggtgggga cctgaagggc cgagttttta tcattggcta
     3241 ccagacaaca ttcgagaaag gcgagggaat ggagactttt acctttgttg agcgggacca
     3301 tcaatggaag ctggcgaggt atcgaataaa ctcaatggcg ctcagataga ttcgaggttg
     3361 cggatcttcg ggtcatgagg ttttaggtgg aggctaactg aa
//
LOCUS       DASUEX010000642         2395 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_538147, whole genome shotgun sequence.
ACCESSION   DASUEX010000642 DASUEX010000000
VERSION     DASUEX010000642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538147"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1064)
                     /locus_tag="VFV61_10675"
     CDS             complement(<1..1064)
                     /locus_tag="VFV61_10675"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEX4900988.1"
                     /translation="MKISAGPARRADDRGPVSADTNDSDEFFKSAVKSGPERDNSQKI
                     EALLKRMTLEEKVGQMTQLTLGMIVTGRDQNIQIDPAKLEKAIVKYGVGSILNVSDQA
                     LTPDKWHDTIKQIQEAATKKTRLGIPVIYGIDSIHGATYVQGATLFPQEIGMAATWNP
                     ELMKRGSEIAAMETRAAGIPWSFSPVLDLGRNPLWPRFWETFGEDPYLAKVMGVAFVR
                     GLEGSDVAAQDRVASSLKHYMGYSFPLTGRDRTPAWIPENYLREYFLPPFAAAVQAGA
                     RTIMVNSGEINGVPGHVNHHLLTGILRDELGFKGLVVTDWEDIKKLVTIWRVAANEKE
                     ATRMAVMAGNDMSMVPLDYS"
     gene            complement(1661..>2395)
                     /locus_tag="VFV61_10680"
     CDS             complement(1661..>2395)
                     /locus_tag="VFV61_10680"
                     /inference="COORDINATES: protein motif:HMM:NF016668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX4900989.1"
                     /translation="SIMIPCPWLPEIADYARKNPEADLGLHLTLTSEWVLYRWGPILA
                     KERVPSLLDGNGYFYLTETEAAAHIDPKEAEAEIRAQIARAKLLGIQPTHLDSHMGTL
                     YQTQALFETLFRVARDNKLLIRVSQEWFAEAPFLPSLLGPDDVVMNTLISIGPGVTAD
                     GWSKFYADAIKNLQPGISEMIVHLAYDDEEMRGVAFNHPDWGAEWRQRDFQFVTSEAF
                     RKLLQENNVKLVTWREISQRLQKRAR"
BASE COUNT          523 a          637 c          642 g          593 t
ORIGIN      
        1 aagctatagt cgagcggtac catgctcatg tcgttgccgg ccatcacagc cattcgggtg
       61 gcttctttct cgttggccgc aacgcgccag atcgttacca gtttcttgat gtcttcccaa
      121 tcagtaacga caagtccttt gaaacccagt tcatcgcgca ggattccggt caagagatga
      181 tgattgacgt gtccaggaac gccgttaatc tctccggagt tgaccatgat cgttcgcgct
      241 ccggcctgaa cggcggccgc gaagggcggc aggaagtatt cgcgcaaata gttttccgga
      301 atccacgccg gagttctgtc tcttccggtc agcggaaaac tgtagcccat gtagtgtttc
      361 aaactggaag ctacacggtc ctgagcagcg acgtccgagc cttccaagcc tcgcacgaaa
      421 gcgacgccca taactttggc aaggtacggg tcttcgccaa acgtttccca gaaacgtggc
      481 cagaggggat tccgacctaa atcgagcact ggcgaaaagc tccaggggat ccctgccgct
      541 cgcgtttcca tggcagcgat ttccgagcct cgtttcatca actcaggatt ccaggttgcg
      601 gccataccga tctcctgggg gaacagcgtt gcgccctgaa catacgttgc accgtgaatg
      661 gaatcgatgc cgtagattac aggaatcccg agacgtgttt tcttagtcgc tgcttcctga
      721 atctgtttaa tcgtgtcgtg ccatttatct ggtgtcaggg cttgatctga gacgttaaga
      781 atcgagccaa cgccgtattt gacgattgcc ttttccagtt tcgccggatc aatttgaata
      841 ttctgatctc gcccagtgac tatcataccg agggtcagct gagtcatctg gccaaccttc
      901 tcttcgagtg tcatccgctt gaggagggcc tcgatcttct gcgaattgtc gcgctccggt
      961 ccgctcttca cggcactctt aaaaaactcg tcactatcat tggtgtctgc ggaaactggc
     1021 ccgcgatcgt ctgcccttcg agccggtccg gctgagattt tcatagtggt ttgctccaca
     1081 ccgccggagc tggaagcttc ttgtagtggc acaccaaagc atattgaaag cgtaacgacc
     1141 gctacgacaa gcgcgttttt tttcatattc ttttcgttcc ttcaggaatc gttctcatcg
     1201 ccacgcatcg gcaggtaggc gtcgggggaa agtctggcat ctcgtcaaaa aaagcgctga
     1261 gaaacttgac cgcctgccaa gcttcactgg tcgtgcgttc cgcgacaagc gggtgaatgc
     1321 ggttcgaata gcgttggagg gtggagtttc gcctatcatc ttgctgacac ggcgtaagtt
     1381 accgtgtcat acgtgagtta tcggcgagca gaaaaatcct cattgggcaa ttatttaagg
     1441 gagccgcaaa agtaacgatg ggaagcagga aaattctaat tatgaagtcg gtcaccacct
     1501 ggcggtagcg ggcgggttgg ggcatttccg attgccgatt gctcattgcc gattgacgat
     1561 taacagccgg aacaacattg caactggcaa tcggcaattg gcactgccgc ggccacccgc
     1621 taccgcgtgg tggtactgac ttcattgaca caaggtggcg ctatcgtgct cgcttctgta
     1681 gtcgctgact gatttcgcgc cacgttacaa gcttaacgtt gttctcctga agcaattttc
     1741 ggaaagcctc actcgtaaca aactggaaat cgcgctggcg ccattctgcg ccccaatcgg
     1801 gatgattaaa cgccactcct ctcatctctt cgtcgtcgta tgccagatgt acgatcatct
     1861 cactgatacc gggctgaaga tttttgatgg cgtcggcgta gaacttagac cagccatccg
     1921 ctgtgacacc gggcccaatg ctaattaagg tattcatcac aacatcgtcc ggacccagga
     1981 gcgaaggcag aaagggcgcc tccgcaaacc actcctgcga aactctgatt agtagcttgt
     2041 tgtcgcgtgc aacgcggaag agagtctcaa acagagcctg agtttggtag agcgtaccca
     2101 tgtgcgagtc gaggtgggtg ggttgaatgc cgagtaattt ggcgcgcgca atctgtgctc
     2161 gaatttctgc ctctgcttct ttggggtcta tgtgcgcagc ggcttctgtt tcggtcaggt
     2221 aaaagtagcc attgccgtcc agaagggaag gaacacgctc ttttgccagg atcggtcccc
     2281 agcgataaag gacccactcg ctcgtaagag ttaaatgcag gcccagatct gcttcggggt
     2341 tttttcgtgc gtagtcagct atctcgggta gccagggaca agggatcatg atgct
//
LOCUS       DASUEX010000643         4575 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1026703, whole genome shotgun sequence.
ACCESSION   DASUEX010000643 DASUEX010000000
VERSION     DASUEX010000643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4575
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1026703"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /locus_tag="VFV61_10685"
     CDS             complement(<1..501)
                     /locus_tag="VFV61_10685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900990.1"
                     /translation="MGIFQKRPAITRFSNRRIGLRSFVFMLALLASATVGLSQTPADT
                     RTPSVPAARATTAAVVDDSARLITEFEVNGLKVLLKRREGNQTVVAGLYIRGGSRNIT
                     AENAGIEALMLDVATEASANFTRERFRRELSRTGTVITYGINYDYAALMLGSTRQHFE
                     RGWEIFA"
     gene            complement(505..1995)
                     /locus_tag="VFV61_10690"
     CDS             complement(505..1995)
                     /locus_tag="VFV61_10690"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX4900991.1"
                     /translation="MELFVKRYLLVLLLISSFVVPTTFASVQSPARPQAGRQAPRSAA
                     VAPAKAAEESVVTKTLANGLEIIVLEDHSIPLVTVEMAFRNGSYTEPPELNGLSHLYE
                     HMFFKANRASLQREDYLQTIDQLGIIYNGQTQEEVVNYYFTTTTPNFGVAMRFMRDAA
                     RYPAFDPVQFAQEREVVIGELDRHESNPYGLIGQVMNDRLFYKYPSRKRPSGFKDTVR
                     AATTDQMRLIQSRYYVPNNAAIVVTGDVNAPAVYALVEQLFGDWERRAKDPFVEFPLV
                     EHPPLPKSSGELLVQPVQNVVIQLGWQGPSIGKDDAATYAADVFSFILRQPNSRFQRA
                     LVDSGLVAGAGIGYYTQRNVGPIFLTLQTTPEKARAAVRAAYAEVAHFNDPNYFTDEE
                     LESAKALLEADDLFSREKLSEYSHVLSFWWASTGFEYYRGYLKRLRATSRADISRYVN
                     TYIQDKPHIGLALLSEDSVRASGLTADDLVGNQNTNTKAAQKAAGK"
     gene            complement(2092..3660)
                     /locus_tag="VFV61_10695"
     CDS             complement(2092..3660)
                     /locus_tag="VFV61_10695"
                     /inference="COORDINATES: protein motif:HMM:NF016190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HEX4900992.1"
                     /translation="MKKLQLQLAGVGRRRQVFAASGVCLLIVLGAVDVFARVGGGQSY
                     GGGGDGGDGDGAAGAILWLVFQLFRVLLYLTIEYPVIGIPLDILVIAGVIYFLWRRGQ
                     SRQPAIFSTAVGTAAATDPRSFSHSDGFVREFAQLQKFDPNFSEIVFTDFSYALFSRA
                     HEARGREAKALDLLSPYLSEPARSSLLQLNPVGLREVKGIIIGSLAVLEVRGLDTPLV
                     QISLLFEANYTEVVQTGGSTTETAYYVRERWDLERKRDVLSPTPAQATALHCPRCGAA
                     LQQDNIGACAFCGTKIDSGEFQWYVQRITPLSREDRGPLLTSDVPEVGTDFPSVVQPG
                     FDHLRFEFEKNHPEFSWTDFQARAVVIFNELQTAWSTLDWERARPHETDNIFQMHQYW
                     IDAYRRQGLRNVLDRFAIHALQPVKIKEDAFYNAITLRIGAQGFDYTVDSNGRVVSGS
                     QNVPRSWSEYWTFIRNRQAKPQAARADLNCPNCGAPLKVNVSGVCAFCGGKITSGEFD
                     WVLSRIEQDESYVG"
     gene            complement(3660..4286)
                     /locus_tag="VFV61_10700"
     CDS             complement(3660..4286)
                     /locus_tag="VFV61_10700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900993.1"
                     /translation="MTSSRIDQAACESLADCVAEIEKHTDAELVIVVRAHSGSYAHAD
                     YLAGALLAFCGLLFLLFSPINFHQYWVVIDVVLLFALGVLLSAKSRSIRRLLTRKKFR
                     EHAVRTGAAAMFYEAGIANTKAELGVLVYLSLLERRLELIADRGVLQVVPSLEWNECL
                     FELHQAGREDDLNSLMKGLRLLGALLAAYLPPTGENPNELSDAPRIEL"
BASE COUNT         1067 a         1301 c         1231 g          976 t
ORIGIN      
        1 ggcaaaaatt tcccagcctc tttcaaagtg ttgacgcgtg ctacccaaca tcagcgctgc
       61 gtaatcgtag ttgatcccgt aagtgatcac cgtccccgtg cgggacaact cgcgacgaaa
      121 acgttcacgc gtgaaattcg cgctggcttc ggtagcaaca tccagcatca aggcttcaat
      181 gccggcgttt tcagcggtga tgttgcgcga gcctccgcgg atatagagcc cggcaacaac
      241 tgtttgattg ccttcgcggc gcttcagcaa caccttgagt ccgttaactt caaactcggt
      301 aatcagacgc gcactgtcat cgacaaccgc cgctgtcgtg gcccgcgcgg ccggaacgct
      361 gggtgtccgc gtgtcggccg gcgtttgcga aaggcccact gttgctgacg ccagcaacgc
      421 cagcatgaag acaaaacttc gcagtccaat ccgcctattg ctgaaccgag tgatcgctgg
      481 tctcttctga aatattccca tcgattattt ccctgctgct ttctgtgccg cttttgtgtt
      541 tgtgttttga ttacccacca gatcgtcagc agtgagtccg gaggcgcgca ccgaatcttc
      601 cgacaatagc gccagaccga tgtgcggttt gtcctgaata taggtgttca cgtagcgact
      661 gatgtcggct cgcgaggtgg cgcgcaagcg cttcagatag ccacggtaat actcgaaacc
      721 cgtcgaggcc caccaaaagc tcaatacatg tgagtactcg gagagcttct cgcgtgaaaa
      781 gagatcgtca gcttcaagca gagccttggc actctcgagc tcttcatcag tgaaataatt
      841 ggggtcgtta aagtgggcca cctcggcgta ggccgcacgc actgcggccc gagccttttc
      901 cggagtcgtt tgcagcgtca gaaaaatcgg gccgacattt cgctgcgtgt aataaccaat
      961 gcccgcgccg gccaccaagc cgctgtcaac cagcgcgcgc tgaaatcgtg agtttggctg
     1021 gcgcagaatg aacgagaaga catcagcagc ataagtggct gcgtcatcct tcccaattga
     1081 aggtccctgc caaccaagtt ggatcacaac gttctgcacc ggctgcacca gcagctctcc
     1141 cgagcttttc ggcagtggcg gatgctcgac gagcggaaac tcgacaaacg gatccttggc
     1201 tcgccgctcc cagtcaccga acagttgttc cacgagcgcg taaacggccg gagcgttaac
     1261 gtcgccggtg acgacgatcg ccgcattatt aggaacgtag tagcgactct ggattagacg
     1321 catttgatcc gtcgtcgcgg cgcggacggt atccttaaag ccactggggc gtttgcggct
     1381 gggatactta tagaacaaac gatcattcat cacctggcca atgaggccgt aaggattcga
     1441 ttcgtgacgg tcgagctcgc cgataactac ttcgcgttcc tgcgcaaatt ggactgggtc
     1501 aaaagcggga taacgagcgg cgtcgcgcat aaagcgcatc gccacgccaa agttgggagt
     1561 cgtagtcgtg aagtagtagt taaccacctc ttcctgcgtc tgaccgttat agataatgcc
     1621 aagctgatcg atcgtctgta ggtagtcttc tctttgcagt gaagcgcgat tggctttgaa
     1681 gaacatatgc tcgtaaagat gggaaagccc gttgagctca ggcggctcag tatatgagcc
     1741 attacgaaat gccatctcca cggtgacgag cggtatcgaa tgatcctcca acacgattat
     1801 ctctaaaccg ttggccagtg ttttggtgac gacactttcc tctgctgctt tggccggggc
     1861 cactgccgcc gagcgtggcg cctgccgtcc ggcctgcggt cgcgccgggc tttgcaccga
     1921 cgcaaaagtg gtgggcacaa caaaggaact aataagtaac agaacgagca gataacgctt
     1981 cacaaaaagc tccatttcgt tttccaaatg tgtctgaaat ccgaggccac gccgcacaaa
     2041 gcattgaccc aggggcgatg cctttcgatg tccgcttacg gtgattggat gtcagccaac
     2101 gtaagattca tcctgctcaa tgcgagacaa gacccagtca aactctcccg aggttatctt
     2161 gccgccgcag aatgcacaaa cgccgctgac gttcactttg aggggggcac cacagttagg
     2221 gcagttgaga tcggctcggg ccgcttgcgg ttttgcctgt cggttgcgga taaatgtcca
     2281 gtactcgctc caggagcgcg gcacattctg cgaaccgctt accacgcggc cgttgctgtc
     2341 cactgtatag tcaaaaccct gcgcgccgat ccgcagcgtg atcgcattgt agaacgcatc
     2401 ttccttgatc tttacgggct gcagcgcgtg gatcgcaaag cgatccagca cattacgcag
     2461 accctgccgg cgataagcgt caatccagta ttgatgcatc tggaaaatgt tgtcggtctc
     2521 gtggggacgc gctcgctccc agtcgagcgt tgaccaggcg gtttgcaact cgttaaagat
     2581 cacaacggcg cgcgcctgaa aatcagtcca ggaaaattcg ggatggttct tttcaaattc
     2641 gaaacgcaga tgatcgaagc ccggctgtac aacgctggga aagtcggttc ccacttcggg
     2701 cacgtcggac gtgagtagcg gaccgcgatc ttcgcgactc agcggagtga tccgctgcac
     2761 ataccattga aactcgccgc tgtcgatctt cgtcccacaa aaagcgcaag cgccgatgtt
     2821 gtcttgttgt aatgctgcgc cgcagcgcgg acagtgaagc gcagtcgcct gcgccggcgt
     2881 gggagacagc acgtcgcgct tgcgctccag gtcccagcgc tcgcgcacat agtaagcagt
     2941 ttccgtagtc gatccgccag tttgcaccac ctcggtgtag ttagcttcaa acagcaaact
     3001 gatttgcacc agcggcgtgt cgaggccgcg aacctccagc accgccagag aacctatgat
     3061 gatgccctta acttccctga gaccgaccgg gtttagttga agcaaggaac tgcgagcagg
     3121 ctcgctcaaa tagggcgaaa gcagatcaag ggcttttgct tcacgaccgc gggcttcgtg
     3181 tgcgcggctg aaaagtgcat aactaaaatc ggtaaagaca atctccgaga agttcgggtc
     3241 aaacttttgc agttgtgcaa actctcgaac gaaaccatcg ctatgagaga agctgcgcgg
     3301 atcagtcgcc gccgcagttc cgactgcggt tgaaaagatt gctggttgcc ggctttggcc
     3361 tcgacgccag agaaaataga tgacacccgc gatcaccagg atgtcgagag gtatgccgat
     3421 tactggatac tcaatcgtga ggtaaagcaa gactcggaaa agttgaaaga ccagccacag
     3481 gatcgctccc gcggcgccat cgccgtcccc gccatcgcct ccaccaccat acgattgtcc
     3541 accgccaacc cgggcgaaga cgtcgacggc gccgaggaca attaacaagc acactccact
     3601 cgccgcgaat acctgtcgcc tacgaccaac acccgcaagt tgtagttgaa gcttcttcat
     3661 cacaactcaa ttcgcggggc gtccgacaat tcgtttgggt tttctccggt cggcggtaaa
     3721 taggcagcga gcaatgcccc gagaagccgc aaccccttca tcagcgagtt gagatcgtct
     3781 tccctaccgg cttgatgcaa ctcaaagagg cattcgttcc attcaagtga gggtacgact
     3841 tgcaacacac cgcgatcggc aatcaactcg aggcgccgct ccagcaacga caggtaaacc
     3901 agcacgccga gctcagcttt tgtgttggca attcccgcct cataaaacat tgcggccgcg
     3961 ccagtgcgga cagcatgctc gcgaaacttc ttcctcgtta gcagccggcg aatgctgcgg
     4021 ctctttgccg aaagtaggac accgagcgca aacagcagca ccacgtcgat aacgacccag
     4081 tattgatgaa aattgatcgg ggaaaacagc aggaacagca aaccgcagaa ggcgagtaag
     4141 gcgcctgcta aataatcagc atgcgcgtag gaaccggagt gtgctcgcac cacgatcacc
     4201 agttcagcat ccgtgtgctt ttcaatttca gctacgcagt cggccagcga ctcacatgcg
     4261 gcttggtcaa tcctgcttga tgtcattggt ggattttggg ggtcggcgct tgggtcccta
     4321 agccagttaa gcgaattcgt agaccactga tccagctgaa ctactcatcc aacgcaagtc
     4381 taacgcaagg tcgaatctga tcatacccgt ggcagggcga cgggcgagac cttccgcttt
     4441 cggagtgcag gcaacttgcc gcttttagac gctgaaaaag tcagaactta tccgaaagcc
     4501 gttaccattt cgttacctga gcgtaaaacg gatttcacat acgactgata ctgtcatctt
     4561 agaggttcta gtttg
//
LOCUS       DASUEX010000644         2811 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1369261, whole genome shotgun sequence.
ACCESSION   DASUEX010000644 DASUEX010000000
VERSION     DASUEX010000644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1369261"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /gene="tsf"
                     /locus_tag="VFV61_10705"
     CDS             complement(<1..380)
                     /gene="tsf"
                     /locus_tag="VFV61_10705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEX4900994.1"
                     /translation="MAEVTAAAVKQLRDKTGAGMMECKNALVEAGGNDEVAVDILRKR
                     GLASAKKREGRIAAEGIVGQYIHMGGKVGVLVEVNCETDFVARGDEFQQLVKDVAMHV
                     AAAEPKYVSRQDVPAVELNKEREIA"
     gene            complement(521..1453)
                     /gene="rpsB"
                     /locus_tag="VFV61_10710"
     CDS             complement(521..1453)
                     /gene="rpsB"
                     /locus_tag="VFV61_10710"
                     /inference="COORDINATES: protein motif:HMM:TIGR01011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEX4900995.1"
                     /translation="MKELLEAGVHFGHQVRRWNPKMKEYIFGERNGIYIIDLQKTQRM
                     FREAITFVTNAIAEDRGKTVLFVGTKRQAQDAIREEAERCGQYYVNQRWLGGLLTNFQ
                     TVQKSIKRLRDLEAMQTDGRYEKLTKKERIKLDREREGLNKNLSGIKAMGRLPDVVFI
                     IDVRKEEIAVAEANRLGIPIVAVVDTNCSPEGIDYVIPGNDDALRAVRLFASRIADSI
                     LDGQQIATEGAVAISTDDEAEFAETAAGDTGATEPAIEPESVTPAVNEATSPAADADS
                     GAIGNPVMPAPEASANNDAEAVNDPSAESVVAAS"
     gene            complement(1620..2012)
                     /gene="rpsI"
                     /locus_tag="VFV61_10715"
     CDS             complement(1620..2012)
                     /gene="rpsI"
                     /locus_tag="VFV61_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEX4900996.1"
                     /translation="MADIQYYGTGRRKTSTARVYLRPGAGDVVVNRKAFDRYFPNETL
                     RMIIRQPLQLTETTAKFDVLINVKGGGPAGQAGAIRHGITRALLEFNADLRPTLKQAG
                     LVTRDPRIKERKKYGQKGARKRFQFSKR"
     gene            complement(2045..2500)
                     /gene="rplM"
                     /locus_tag="VFV61_10720"
     CDS             complement(2045..2500)
                     /gene="rplM"
                     /locus_tag="VFV61_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019394039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEX4900997.1"
                     /translation="MSTYFPSGKDLAAKRKWHVVDAAGQTVGHLASEVASILTGKRNP
                     QWTPFMDMGDHVIVINARKAVLKGSKVEQKVYRHHTLYPGGLREVSAKEMQAKNPERI
                     IELAIRGMLPKTKLGKAMGKKLKVYPDAEHPHSAQRPEPRQIKSRSNQK"
BASE COUNT          550 a          833 c          711 g          717 t
ORIGIN      
        1 gcgatttccc gttctttgtt taactcgact gccggaacgt cttgccgcga aacatacttc
       61 ggttcggccg ctgccacgtg catagccacg tctttcacta gttgttgaaa ctcatcaccg
      121 cgggcaacga agtcggtctc acaatttacc tcgacgagca cacccacctt gccgcccata
      181 tgaatgtact gcccgacgat cccttcggcc gcgatacgtc cctcgcgctt cttggcggac
      241 gccaacccgc gcttgcggag gatgtctacg gcaacttcat cattgcctcc agcctcgacc
      301 agagcgttct tacactccat cattcccgcg cccgtcttgt cgcgaagctg ttttactgct
      361 gctgctgtta cttctgccat gatcacaaaa ttctcccgat ttaagcttcc attcttgggt
      421 cctgcaaact ggggtttctc ggaccgtaac gaaaaacgca gcccggcgga agtccgctaa
      481 aggctgcgct cgccgatgag tacaaatcta aaactattgt tcagcttgct gccacaactg
      541 actcagcgct cggatcgttg actgcttctg cgtcgttgtt tgccgaagct tctggagcag
      601 gcatcaccgg attgccaatc gcgccgctgt cggcgtcggc cgccgggctg gtcgcttcgt
      661 taaccgcagg tgtcacggat tccggttcaa tcgctggttc cgttgcgccg gtatcaccgg
      721 ccgctgtttc agcaaactca gcttcgtcgt ccgtgctgat cgcaacagcg ccctcggtgg
      781 cgatttgttg accatccaga attgagtccg caatgcgcga cgcgaagagt cgcaccgctc
      841 gcagcgcgtc atcattaccg gggatgacgt aatcgattcc ctcgggcgag caatttgtat
      901 caaccacggc aacgatgggg atgcccaggc gatttgcctc ggcaaccgca atctcttcct
      961 tgcgcacgtc gatgatgaag acgacgtcgg gcaggcgacc cattgcctta attcccgata
     1021 ggttcttgtt cagcccttca cgttcgcggt cgagcttgat gcgctccttc ttcgtcagtt
     1081 tctcgtaacg gccgtccgtc tgcatcgcct cgagatcgcg caggcgcttg atcgactttt
     1141 gcaccgtctg gaaattggtc agcagtccgc ccagccagcg ctggttaacg tagtattgtc
     1201 cgcagcgttc agcttcttcg cggatcgcgt cctgcgcctg tcgttttgtg ccgacgaaga
     1261 gcacggtttt gccgcgatct tcagcgatcg cattggtgac aaaagtgatt gcctcccgaa
     1321 acatgcgttg cgttttctgg aggtcgataa tgtaaatgcc gttgcgctca ccgaagatgt
     1381 attccttcat cttcgggttc cagcgcctta cctggtggcc gaaatgtacg ccggcctcca
     1441 gaagttcttt catcgtaacc gaaaccaagc taagtctcct ttgtgtgaac tggtttagtg
     1501 tagtcgccgc ggctcatagc actctcaatg gagagcaacc aatccgagcc ccgacaatgg
     1561 attccagcaa ttgccgattg gcgattgccg ctttccgatg aagatcggaa agcccttctt
     1621 tatcgtttcg agaattggaa acgtttccgc gcgcccttct ggccgtattt cttccgttcc
     1681 ttgatgcggg gatcgcgcgt aaccagtccc gcctgtttca gggtcgggcg caggtccgca
     1741 ttaaattcca gcagtgcgcg agtgatgccg tgtcgaatcg ctccggcctg tccggcgggt
     1801 ccgccacctt tgacgttgat cagcacgtca aacttcgcag ttgtttccgt caattgcagt
     1861 ggctgacgaa taatcatgcg caacgtctcg ttgggaaaat accgatcaaa ggccttccgg
     1921 ttgacgacta cgtcgccagc accgggccgc agatagactc gcgcagttga agtttttcgt
     1981 cttcctgttc cgtagtattg aatgtcagcc acagctaaat tgtcctctta atcttactca
     2041 gcttttattt ttgatttgaa cgggacttga tctggcgggg ttccggacgc tgcgccgagt
     2101 gtgggtgctc ggcgtcgggg tagactttaa gttttttccc catcgccttg cccaacttgg
     2161 tcttgggcag catcccccga attgccagtt cgattatacg ttcaggattc tttgcctgca
     2221 tctctttcgc tgaaacctcg cgcagcccgc caggatagag cgtgtgatgg cggtagacct
     2281 tctgctcaac cttcgaaccc ttcaagacag ccttgcgcgc attgatcaca atcacgtggt
     2341 cgcccatgtc cataaacggt gtccactggg gattacgctt gccggtcagg atcgatgcca
     2401 cttcagacgc cagatgacca accgtttgcc cggctgcatc caccacgtgc cattttcgtt
     2461 tggcggcaag atccttaccg ctgggaaagt atgttgacat agttaactat ctctattttc
     2521 aggtactaaa acgcgaacgg ctagaataca aacggctcca aaagttgtca agttgagcag
     2581 tttcaaggta gtgcgcttcg ccctcctgct caacaccgcg gcgctgagac gctaagacgc
     2641 aaagaaccag ctacgattga aggacgagtc acccccgcag aactcgttca tttgaaagag
     2701 caaccttgcc gttaatctca atatcgcttt ggctctcgtt gcgactctgc gactctgcga
     2761 ctctgcgact ccgcggctct gcggcagttt ctatacaaac caaaaggctc c
//
LOCUS       DASUEX010000645         5764 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1026974, whole genome shotgun sequence.
ACCESSION   DASUEX010000645 DASUEX010000000
VERSION     DASUEX010000645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5764
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1026974"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..673
                     /locus_tag="VFV61_10725"
     CDS             <1..673
                     /locus_tag="VFV61_10725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4900998.1"
                     /translation="LPPGLHNESNELFGFFVYELRVGHSNRIWSTAQGRFGHPIRVNG
                     VQHPAPPLKCLVNRTPKGLSVTAQYATSLFNGRNVTSKPPKTEIWCMLYAQLRQADGN
                     DNRNILLSELRLDYVDPDKVPRIVDKKWTKLMVANSLLVNLDAPATGTQGWTEAQIQN
                     MLSQFNLAHDTRLSVLAVEMMPRYDQFILFGQTPNQSVRPLSHDLGQYRILRTSRLVS
                     APVVC"
     gene            987..3245
                     /locus_tag="VFV61_10730"
     CDS             987..3245
                     /locus_tag="VFV61_10730"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 family protein"
                     /protein_id="HEX4900999.1"
                     /translation="MQTSLLRRVRPISHRVFASFQVALLIVIAGAVTSLQAASKPPIL
                     ITEAAPSTRAVAFESVTMKPEPFSPTSTVQFATDNRTRIVIFARDLELYAGEGANAFS
                     ADAEDGTGKKYLLRVEYVGRVPGFEGITMMIIRIADDLGNVGDVLLRVNLHGMASNRV
                     RVAMGSIGGGPAVDPLTEYSSPAPATPPPPDPIATPDPFTGPATDADTVRFLEQATWG
                     VPSQAEINRVKAMGFRAYLDEQFALTPTNPAKGSNYPDLPFNVVDDQGTACPTSTGDP
                     NYSQAVCNRDNFSLYPIQRTFFSNALYGQDQLRQRVAFALHQILVVSGANPLNRASWM
                     TIYLQSLDRGAFGNYRTLLGEITLNPEMGEYLDMRISTRTSPNENWAREVLQLFSIGV
                     NELNLDGSPKVDAQGVNIPSYSQTTVNEFTKLFTGWNLAPAIAAGINNWRDPMVPRAG
                     TNHDVTAKVLLNGVTIPACTAGSQACAQSDLAAGLDNIFAHPNVGPFIGRQLIQHLVT
                     SNPSRAYVERVAKVFNNDCDALYPQGCSGVRGNMRSVVQAILLDPEARGDVKTDPNYG
                     KLREPAQYIIGVLRALNVKSFDKSSTSDGVLGSRSSVDLTGSLDQIIFQPATVFSYYQ
                     PEYEVPGAKILGPAFGILSTSTTLKRANNINTLVYSGIQANTTPAQSPDRPRGTSVDL
                     ANIEAMASNPGQVADYLNALMLHGTMSTQFRNSIITAMNAIPGSDANFARKRAQVAAY
                     LVATSSQYDIQR"
     gene            3250..4668
                     /locus_tag="VFV61_10735"
     CDS             3250..4668
                     /locus_tag="VFV61_10735"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX4901000.1"
                     /translation="MATSRRNFLKTSACALGGMAMASTMDSLGLVQALTPQAATGYKA
                     LVCIFLNGGNDGNNMLVSLDQYASYSAARSAAGLALSTGGATPLLPISPLTGGSYAFH
                     PSMPEIQALFNQGHVAALVNNGPLVEPLTRTTYQNGTGKKPLQLFSHSDQVGLFATAI
                     ANNVSQTGWGGRIGDKTTGLNGSATFPSNISIAGVNLFLTGVDTRQLAVSDSNTTLAN
                     VLQLNNAPGATSGEISSRSTSFNDLRMLDNSFKLIKAASDTRSSAIQTDIALSSVNPV
                     LTTPFPSPSTSLSRQLLQVARLIKASTDPAAGINMKRQIFFCQIGAFDTHSNQIAGQA
                     ALLQQVSQAMNAFYNATVELGLQDSITTFTLSDFGRTLQPAGSGAGIVGSDHAWGNHH
                     LIMGGAVSGNRLFGTYPTLQLGGPDDTDGGSSPRGRWIPTTSVEQYAATLATWYGLSS
                     SDLPAVFPLIGRFSTPNLGFMM"
     gene            4772..>5764
                     /locus_tag="VFV61_10740"
     CDS             4772..>5764
                     /locus_tag="VFV61_10740"
                     /inference="COORDINATES: protein motif:HMM:NF041895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor V domain-containing protein"
                     /protein_id="HEX4901001.1"
                     /translation="MHFACKPKYENARLIVFPSLLAAVLGLLLSNNTTNIPVHAFSAG
                     PPAGYTGAPGEEPEACAECHVPADAGTGNISINAPQNYVPGQTYTITVAHNNAADATR
                     LRWGFELTALDTGDEKAGNLQSTDVFTQVLDNQGPGSSRQYIEHTSVGTFVGQQHSAS
                     WTFNWTAPPNDVGTIYFYAAGNQANNDNNTSGDYIYKTFVISSPVSATPDFAVTVAPS
                     IRTITPGGSTTYAVTVTPLAAFTGSVNLSTGTLPAGVSANFVPAVVNLSDASSQGSTL
                     TLNSTAGTPLGSHLVNISGQSGMLTHTAPVTLRVVSPASVDLAVSKTASPNPGQV"
BASE COUNT         1287 a         1655 c         1524 g         1298 t
ORIGIN      
        1 acttccgccc ggacttcaca acgagtcaaa cgagttgttc ggtttctttg tttatgaact
       61 gcgagtcgga cactcaaaca gaatctggtc caccgctcag ggtcgctttg gtcatccaat
      121 tcgcgtcaac ggcgtacagc acccggcgcc accacttaag tgtttggtca atcgcacgcc
      181 gaaagggctt tccgtgacgg cgcaatacgc aacctcgcta ttcaacggac gcaatgttac
      241 gagcaagcca ccgaagacgg agatctggtg catgctctat gcacaactgc gacaggcaga
      301 tgggaatgac aatcgcaata ttctgctatc tgaactgaga ttggattacg tcgatccgga
      361 taaggtaccg aggatagtcg ataagaagtg gacgaagtta atggtggcca attcgctgct
      421 ggtgaatctg gatgccccgg cgaccggaac ccagggctgg accgaagcgc agattcaaaa
      481 catgctctct cagttcaatc tcgctcatga cacgcgcctg agcgtgctcg cggtggagat
      541 gatgccgcgc tacgatcagt tcattctctt tggccaaact ccgaaccaat cggttcggcc
      601 tctctctcac gacctgggcc aataccgtat tttgcggacc tcccggttgg ttagcgcccc
      661 agtagtctgc tgatggattt ctacctcgcg gttgaggccg ggccaaaatc aggagcgccc
      721 tgcgaacgat tccatagttc gagactttgg gcagactgac gacgctcgtt tcgagcgcca
      781 aaactgggaa ctctggttcc actggaccaa ctcgccaaga attcttcggc cttattcccg
      841 ccgcattcgt ctaattgttg acccggggcc tgtcagccat ggtatagtcc cgttcaaccg
      901 ggtttggccg ggtgccccgc cacgtaagtt aagtaatacc tcacagcttt ttgcttcagt
      961 ttgatcttgt gacggagagc ccacttatgc agaccagtct ccttaggcga gtcaggccca
     1021 tctctcatcg tgtttttgct agcttccaag tcgccctgct aatcgtcata gcgggcgccg
     1081 tcacgtcgct ccaggctgcc agcaaaccgc caatcctgat caccgaggca gcgccgtcga
     1141 ccagggccgt cgcctttgaa tcggtgacaa tgaagcccga gcctttttcc ccgacgtcca
     1201 cagttcaatt cgccacggac aatcgcaccc gaatcgtaat cttcgctcgc gatctggagt
     1261 tgtatgcggg tgagggcgcc aacgcatttt cggccgatgc ggaagatgga accggcaaaa
     1321 agtatctact gcgagtcgaa tatgtaggtc gcgtgccggg ttttgaaggc atcacgatga
     1381 tgatcatccg catagcggat gacctcggca acgtaggtga tgtgctgctt cgcgtcaacc
     1441 tgcacgggat ggcaagtaac cgcgtgcgcg tcgcgatggg gagcatcggt ggtgggcctg
     1501 cggttgatcc tttgactgag tattcatctc cagccccggc cacgccgcca ccgccggatc
     1561 cgatcgcgac tcccgatcct tttactgggc cagctacgga cgcggacaca gtacgttttc
     1621 tcgagcaggc gacttgggga gttcccagtc aagctgagat caatcgtgtc aaagcgatgg
     1681 gcttccgtgc ttatcttgat gaacagttcg cccttacgcc gacgaacccg gcaaaaggtt
     1741 ctaactatcc ggatcttccc ttcaacgtag ttgatgacca ggggacggcg tgtcccacca
     1801 gcactgggga tccgaactac agccaggccg tctgtaaccg cgacaacttt tccctttatc
     1861 cgattcaacg aaccttcttc tcgaatgcgc tgtatggcca ggatcagttg cgacagcgtg
     1921 tggcttttgc gctgcaccag attctggtcg tctccggcgc caatcctcta aatcgcgcca
     1981 gttggatgac gatctatttg cagtcgctcg atcgcggcgc ttttggtaac taccgcacgt
     2041 tgttgggaga gatcaccctc aacccagaga tgggcgagta cctcgatatg cgcatcagta
     2101 cgcggaccag ccccaatgag aactgggctc gtgaggtgct gcagctattc tctatcggag
     2161 tcaatgagct gaaccttgac ggctcgccga aggtcgatgc ccaaggcgtc aatattcctt
     2221 cctactcgca gactacggtt aatgagttta cgaagttgtt tacgggttgg aacctggcgc
     2281 ccgcgatcgc tgccggaatc aataactggc gcgatccaat ggtgccaagg gcgggaacta
     2341 accatgacgt caccgcgaag gtgttgctga acggagtgac gattcccgcc tgcactgccg
     2401 gcagccaggc ctgtgcgcaa tcagatcttg cagcgggact cgacaatatc tttgcgcatc
     2461 ccaacgtcgg tccttttatc ggccggcaac taattcaaca tctggtcacc agcaacccta
     2521 gtcgcgctta cgttgaacgg gtagcgaaag ttttcaataa cgactgcgat gcgctgtacc
     2581 cgcaaggttg cagcggtgtt cgcggcaaca tgcgttcggt tgtgcaggcg atcctgctcg
     2641 atccggaagc tcgcggcgac gtgaagaccg atccaaacta cggcaaactt cgcgagccag
     2701 ctcagtacat cattggtgtg ttgcgagcac ttaacgtgaa gtcgtttgat aagagctcca
     2761 ccagcgatgg cgtactggga agcagatcct cggtggacct taccggctcg ctcgaccaga
     2821 tcattttcca accggccaca gtattcagtt attaccagcc tgaatatgag gttcccggcg
     2881 cgaagattct tggcccggcg tttggcatcc tctcaacttc aacgacgctg aagcgcgcaa
     2941 ataacataaa cacactcgtc tatagcggga ttcaggcaaa cacaacgcct gctcaaagtc
     3001 cggaccggcc gcgcggaact tcagtcgacc tggcgaacat cgaagcgatg gcgagcaatc
     3061 ctgggcaagt tgccgactac ctcaacgccc tgatgcttca cggtacgatg tccacgcagt
     3121 ttcgcaattc gatcattacg gcgatgaatg cgatccctgg ttccgacgca aacttcgcac
     3181 gtaaacgagc gcaggttgcg gcttacctcg tggcaacttc atcgcagtac gacatccaga
     3241 gataaaaaca tggctacatc acgcagaaat tttctgaaga caagcgcttg cgcattgggt
     3301 gggatggcga tggcttccac aatggacagc ctcggacttg ttcaggccct aacgccccag
     3361 gcggcgactg gttacaaggc gctggtgtgt atttttctga acggcggcaa tgatggaaac
     3421 aacatgctgg tctcgttgga tcagtacgca tcttattcag ccgctcgcag cgcagccggt
     3481 ctggctctct caactggtgg agctacgcca ttgctcccca tttcgccctt gactggcggc
     3541 tcatatgctt ttcatccgag catgccggag attcaggcgt tatttaatca ggggcatgtt
     3601 gcggcgctcg tcaataacgg gccgctggtt gagccgctga cacgtacgac ttatcaaaac
     3661 ggcactggca agaaaccgct gcagttgttc tcacattccg atcaggtggg actatttgca
     3721 acggcaattg ccaataacgt cagccaaaca ggctggggcg gccggatcgg agacaagacg
     3781 accggtctaa acggctcggc gacgttccct tcaaacattt ccatcgccgg cgtgaatttg
     3841 tttttgacag gagtcgacac cagacaatta gctgtcagcg attcaaatac gaccctcgcg
     3901 aatgtgctgc aactcaacaa cgcgcccgga gccacctccg gagagatttc ctcgcgcagt
     3961 acgtcgttca atgatctgcg catgctcgac aacagcttca aactgatcaa ggctgccagc
     4021 gatacccgca gcagcgcgat tcaaacggac atcgcgctca gctccgtgaa tcctgtattg
     4081 actactccat ttcctagtcc cagcacttcg ctcagccgtc agcttctgca agttgcgcgt
     4141 ttgatcaagg ccagcactga tcccgcagcc gggatcaaca tgaagcgaca gatcttcttc
     4201 tgtcagatcg gtgcctttga tacgcactca aatcagatag ccggccaagc cgcattgctg
     4261 cagcaagtga gtcaggcgat gaatgctttt tacaacgcca ccgttgaact gggactgcag
     4321 gacagcatca cgactttcac actctctgat tttggccgca cgctgcaacc tgcaggtagc
     4381 ggcgcgggaa tagtaggaag cgatcacgcg tggggcaatc accatttgat tatgggaggt
     4441 gccgttagtg gtaatcgact cttcggaacc tatcccacgt tgcagctagg tggacctgat
     4501 gatactgatg ggggctcatc accgcgtggt cgctggatcc ccacaacgtc agtggagcag
     4561 tacgcggcga cgcttgcgac ctggtatggc ttgtcgtcaa gtgatttgcc ggcggtgttc
     4621 ccgttgattg gtcgcttctc gacccctaac ctcgggttca tgatgtaacg gccgccgcga
     4681 gctttttgta atctgctaaa gtgaaatagc ctggcgatat tcgctaggct atttttcaag
     4741 agaactagtt tgcgccccga gcggaatctc aatgcacttt gcctgcaagc ccaaatacga
     4801 aaacgcgcga ttaattgtct ttccctcttt gttagcggca gtgctgggat tgctgctgtc
     4861 gaataacaca accaacattc ccgtccacgc attctcggct ggtccgcccg ctggttacac
     4921 cggagcgcca ggcgaggaac ccgaggcctg tgccgaatgt catgtgcccg ccgatgcagg
     4981 cacgggaaat atttccatta acgcgccgca aaactacgtc cccggtcaga cctatacaat
     5041 cacagtcgct cataacaatg cagccgacgc gacgcgtctg cgttgggggt tcgaactcac
     5101 ggcgttagat acaggcgatg agaaggccgg gaacctgcaa agcaccgacg tgtttactca
     5161 agttctggac aatcaaggtc cgggtagcag tcgtcaatac atcgaacaca cttccgtcgg
     5221 cacctttgtt ggtcaacaac actcggccag ttggactttc aattggaccg ccccaccgaa
     5281 tgacgtggga acgatctatt tctacgcagc agggaaccag gcaaataacg acaacaacac
     5341 ttcgggcgat tacatctaca agacattcgt gatttcttct ccggtctcgg ctacacctga
     5401 ctttgctgtc acggtcgcac cttcgatccg gacaatcaca ccgggaggat cgacaaccta
     5461 tgccgtcacc gttaccccgc ttgctgcatt tacgggaagt gtgaatctca gcaccgggac
     5521 tctgcctgcg ggagtttccg ccaactttgt tccagcagtg gttaacctca gcgatgcaag
     5581 ttcgcagggg agtacattga ctcttaactc gaccgctgga acacctctcg gcagtcacct
     5641 ggtcaacatt tcgggtcagt caggaatgct gacgcataca gcgccagtga ctctgagagt
     5701 cgtaagtcca gccagtgttg atctcgcagt cagcaagacc gcttcaccga acccaggaca
     5761 ggtt
//
LOCUS       DASUEX010000646         3017 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_329242, whole genome shotgun sequence.
ACCESSION   DASUEX010000646 DASUEX010000000
VERSION     DASUEX010000646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3017
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329242"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..613
                     /locus_tag="VFV61_10745"
     CDS             <1..613
                     /locus_tag="VFV61_10745"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX4901002.1"
                     /translation="IYESKGGAELITSGTKYYRGYDPLTGKELWRMADGVDVKIPTPI
                     AANDLYFLGGGSSHTRKAFYAVRAGVKGEIKPADASVGTIAWQSPQIRPHIVTPIVYQ
                     DYLYVCTDNGILSQYEAKTGEPTFRARLGSGGSFSASPVAADGKLYFANEDGEIFVVK
                     AGTKFELLAKNPTGEVLFATPAIAGKLIIIRGQNHVFGIAARD"
     gene            complement(654..1223)
                     /locus_tag="VFV61_10750"
     CDS             complement(654..1223)
                     /locus_tag="VFV61_10750"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEX4901003.1"
                     /translation="MDRRRNMLIGIVVGILLVGASIYATGLSKKLLVPLFGSSAEYKS
                     SESANAPELAPGDWINSEPLKLNELRGRVVLIEFWTFGCINCRNTLPFIKNLDDRYRQ
                     QGLTVIGVHSPEFDEERKVEHLRREVSSLGIRYAVVTDNDYQTWNAYKVEAWPTVFLL
                     DKRGRIRWMHVGEGDYDEAERQIQKLLAE"
     gene            complement(1302..2219)
                     /locus_tag="VFV61_10755"
     CDS             complement(1302..2219)
                     /locus_tag="VFV61_10755"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEX4901004.1"
                     /translation="MANPILVTGAAGRIGGVGRTVTELLLQQGKAVRAMVRSEDQRAQ
                     TLRDMGAEVVVGDLLDLDSMHRVISGCETMYLGMSVSNDYLAATVNTAAVAKHHGVKA
                     LINMSQMTVAQMSINETTTSPQHKLHWLAEQALNWSGLPVVHVRPTVLLEGFFLIFTA
                     DSVRESNEIRLPFGDGKTSPVAVEDVARVIAALLANPQPHLGKIYHLTGPQSENMHFF
                     AEEYSQALGRTITFHDIPVEAWRNGLLERGLPAHLVNHLATMADLHRAGRYDRRSDDV
                     LTLTGRAPLSLQDIVRKNAAIFTASAKGA"
     gene            complement(2291..>3017)
                     /locus_tag="VFV61_10760"
     CDS             complement(2291..>3017)
                     /locus_tag="VFV61_10760"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX4901005.1"
                     /translation="FRWKLIGGFMEATKLTKAKTVNMKLEVVLLGVSDVDRAKAFYEN
                     LGWRLDIDIAAGDFRGVQMTPPNSETSIIFGKGITSNKPGLAPSLVLAVDDVAAARDD
                     LIARGVNVSEVFHYAAGPFNNTVDDPRVSGRDPEGRSYFSFASFEDPEGNGWLFQEIT
                     TRLPGREWNSKRAQAIDAATLAQLLRETGEHHDHFEKTHAEHHWWDWYGSYLSARQNG
                     STPEQASAAADRYMEDVLHVLPR"
BASE COUNT          659 a          859 c          818 g          681 t
ORIGIN      
        1 catttacgaa agcaagggtg gcgctgaatt aattaccagc gggacgaaat actatcgcgg
       61 ttatgatccc ttgacgggca aagagttatg gagaatggcg gatggcgtgg acgtcaaaat
      121 cccgactcct attgcagcaa acgacttgta ctttctaggc ggtggttcct cccatacgcg
      181 aaaagcattt tatgccgtgc gtgctggagt caaaggagag attaagcccg cggacgcgag
      241 cgttggtacc atcgcctggc aaagcccgca gattaggccg cacattgtca cgccgattgt
      301 ctaccaggat tatttgtacg tctgcacaga caacggcatc ctgagtcagt acgaagccaa
      361 aaccggcgaa ccgacgtttc gcgcacggct gggcagtggc gggtcgttta gcgcatcacc
      421 ggtggccgcg gacgggaaac tctattttgc gaacgaagat ggtgaaatat ttgttgtcaa
      481 agcgggtact aagtttgaac tgctagccaa aaatcccacc ggagaagtcc tatttgccac
      541 tcccgcgatc gcaggcaagc tgatcatcat tcgcggccag aatcatgtct tcgggattgc
      601 ggcaagagat tagagaagca atgtaaaggg cttttgtact tggcctactg ttcctattcg
      661 gcaagtaact tctgaatctg tcgctcggcc tcgtcgtagt ccccttcacc aacgtgcatc
      721 cagcgaattc gtccccgctt atctaacaga aagaccgttg gccaggcttc gaccttgtaa
      781 gcgttccatg tttggtagtc gttatctgta actaccgcgt accgaattcc caacgaagag
      841 acttcacggc gaaggtgttc gacttttcgt tcctcatcga attcaggtga gtgtacgccg
      901 attacggtaa gaccctgttg tcgatagcga tcgtccaggt ttttgatgaa cggcaacgtg
      961 ttacgacagt tgatgcagcc gaaagtccag aactcgatta gtacgacgcg accacgcagt
     1021 tcattaagct tgagcggttc ggagtttatc cagtcaccag gagccagctc tggagcattg
     1081 gcagattccg aacttttgta ttcagcggag cttccaaaaa ggggaacaag taacttcttt
     1141 gacaaacccg tagcgtagat cgaagcccct acaagaagga tccccacaac aatcccgatt
     1201 aacatatttc gtcgtctgtc catagatcac tttctgagct tcccttcatt ccttgctgcg
     1261 gtgttaccca gatcaagatc cgcaagggca tacgcagaac ctcaagctcc ttttgccgac
     1321 gcagtgaata tcgccgcatt cttcctgact atatcctgca ggctcagtgg cgctcgccct
     1381 gttagcgtga gcacgtcgtc cgacctgcgg tcgtagcgac ccgcgcggtg cagatcggcc
     1441 atcgtcgcga ggtggttcac caggtgagcc ggcaagccac gctcgagcag tccgttccgc
     1501 cacgcttcga ccggaatatc gtgaaaggtg atcgttcggc ccagtgcctg cgaatactcc
     1561 tcggcaaaga aatgcatgtt ctcagactgc ggaccggtca ggtgataaat cttgccgagg
     1621 tgcggctgcg gattcgcaag cagcgcggcg atcacacgtg ccacgtcctc cactgcgact
     1681 ggcgaagttt tcccgtcacc gaacggtaac ctgatctcat ttgactccct gaccgaatcc
     1741 gcagtgaaaa tcaggaagaa gccttcgagt aacacagtcg gccgcacgtg cacgaccgga
     1801 agaccggacc agttcaacgc ctgctcagcg agccagtgta gcttgtgctg cgggctcgtg
     1861 gtcgtttcgt taatgctcat ctgtgcgacc gtcatctgcg acatattgat cagtgctttc
     1921 acaccgtgat gcttggccac cgctgccgtg ttaaccgtcg cggccagata gtcatttgaa
     1981 accgacatgc ccaagtacat cgtctcgcaa ccggaaatca cccggtgcat cgaatcgaga
     2041 tcgagcaggt cgccaaccac aacctcggcc cccatgtcgc gcaaggtctg cgcgcgttgg
     2101 tcttcacttc gtaccatcgc acgcaccgcc ttgccttgct gcaatagcag ttcggtgacc
     2161 gtgcgcccta ctccgccgat gcggcccgcc gcgccggtga ccagaatggg attcgccatg
     2221 tttactccta aacggtcacc ttagtcgctg gtccgatctg ttgcaggaag ctgcgactgc
     2281 cccaacagcg tcatcttgga agaacatgaa gaacgtcctc catgtagcgg tcggcggccg
     2341 cagaggcctg ctccggcgtg ctgccatttt gacgggcgct tagataggaa ccgtaccagt
     2401 cccaccagtg atgctccgca tgcgtcttct cgaagtggtc gtggtgctct ccggtctcgc
     2461 ggaggagctg tgcgagtgtg gcggcgtcta tcgcttgggc tcgctttgaa ttccattcgc
     2521 ggccgggaag ccgcgtcgtg atctcctgga acaaccagcc gttaccctct ggatcctcga
     2581 acgaggcaaa cgagaagtag gaacgaccct cggggtcgcg tccgctgacg cgcggatcat
     2641 ccacagtgtt gttgaagggg ccggcggcgt agtgaaagac ctcgctcaca tttacgccac
     2701 gggcaatcaa gtcgtcgcgg gcggcagcaa cgtcgtccac ggcgagaacc aggctgggtg
     2761 ccaatcccgg cttgttcgac gtgattccct tgccgaagat gatcgaggtt tccgagtttg
     2821 gtggcgtcat ctgcacgccg cggaagtcgc ccgcggcgat gtcgatgtcg agccgccaac
     2881 cgagattctc gtaaaaggcc ttcgcgcgat cgacatcgga aacgccgagc agcacgactt
     2941 cgagcttcat atttaccgtt ttggcttttg taagttttgt agcttccata aatcctccta
     3001 tcaatttcca gcgaaaa
//
LOCUS       DASUEX010000647         2445 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_197933, whole genome shotgun sequence.
ACCESSION   DASUEX010000647 DASUEX010000000
VERSION     DASUEX010000647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197933"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1403
                     /locus_tag="VFV61_10765"
     CDS             <1..1403
                     /locus_tag="VFV61_10765"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEX4901006.1"
                     /translation="VRHALEKEVEARIDSIPNFLRELHAALNSSPVVATALRETVAMD
                     PNRTIASVTQPEIKKTTNLSDTAVQPHIAQETNFGTSFDSLAGNVSTPQLDKQREEEE
                     RLSKEKQERERREKEHQEKFDRMSLQAKELEDRLERLSGSMPPALDPETTRVQGTVTN
                     PDWVGNPATARPVGPGKQMIVDFSPQRKGPPPLLYIGAAVFVFLLAGVGITAYLMLQP
                     GVRTGDPTPTPTPVTPPKAKAELLPISGGTFMMGRKSGPPQETPAHAVTVQGFSMDRT
                     EVSNTEYADFVRETNHAAPAHWIGNKPPFGQEMWPVVNVSYDDATTFAAWRSKRDGVS
                     YRLPTEEEWEYAARNGEQSDLYPWGPDWKDKAAVLKDATPAAVGSRAEGKNKWGVFDL
                     IGNVWEWTSSKASVYPGNPATVPSSIQEWVTIRGGCYVSDPAKSDAPVSSCLREFVPS
                     STKTTLLGFRLVRSGS"
     gene            1501..>2445
                     /locus_tag="VFV61_10770"
     CDS             1501..>2445
                     /locus_tag="VFV61_10770"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX4901007.1"
                     /translation="MSINAILRAAFLSSAALCLSLCTVASVHGQDVGIDPGGSGLFRA
                     KNPETKKSASKPGTGAKPANRNSAAARANTERIEDLLEKGNEARDARKFADAETAYKE
                     VLKLKPRDARAAYGLGNIYSDQQRWDDAEAAYRNSVAWAPTNADAYVALSVVLVQPSS
                     GGDNARRLVDAESFARRSVQIDPKNAVGWDRLGVALQARGLANSETEHSYRRAIELDP
                     QFAVAYAHLARMLNRSGRQAESTPLYEKAIELAKDPATLNLIADSLQGEQLWPQSEPV
                     LKRALELDSSNPTSLVLMGRYLIVYKRYQEAEPYLKSAT"
BASE COUNT          567 a          667 c          729 g          482 t
ORIGIN      
        1 tcgtgcgcca cgcactcgaa aaggaagtcg aagcgcgcat cgattcgatt ccaaactttt
       61 tgcgagagct ccatgcggcg ttgaactcct cgcctgtggt tgctacggca ctgcgagaaa
      121 cagtggcgat ggatccgaac cggaccatcg cttcagtcac tcagcctgag atcaagaaga
      181 ctaccaatct ctcagacaca gcagtacagc cccacatcgc gcaggaaacc aacttcggca
      241 cttccttcga ctcgctcgcg ggcaatgtca gcaccccgca gttggacaaa caacgcgaag
      301 aagaagagcg cttaagtaag gaaaagcagg aacgcgagcg acgcgaaaaa gagcaccagg
      361 aaaaattcga tcgcatgtcg cttcaggcga aagagcttga agacaggctc gagcgtctgt
      421 ctggcagcat gccgccggca ctcgatccgg aaacgacgcg agtgcaggga actgtcacga
      481 atcccgattg ggttggtaac ccagctacgg cgcgtccagt tgggccaggc aagcagatga
      541 tcgtggactt ttcgccgcag cggaaaggtc cgccaccact gctgtacatc ggcgccgcgg
      601 tatttgtttt cttactcgcc ggtgtcggca tcactgctta tctgatgttg caaccaggtg
      661 ttcgcaccgg cgatccaaca cccacgccaa cgcccgtcac accgcccaaa gcaaaggccg
      721 agctactgcc aatcagcggt ggaactttca tgatgggacg caagagtggt ccaccgcagg
      781 aaactccggc gcatgcagta actgtccagg gcttttcgat ggaccgcacc gaagtgagca
      841 acacggaata tgccgacttc gtcagagaga cgaaccacgc cgcgccggct cactggatcg
      901 ggaacaagcc gccgtttggt caggaaatgt ggcccgtcgt gaacgtctcg tatgacgatg
      961 cgactacttt tgcggcgtgg cgctcgaagc gcgacggcgt tagctatcgt ttgccgaccg
     1021 aagaagaatg ggagtacgcc gcgcgcaacg gtgagcaaag cgatttgtac ccgtggggac
     1081 ctgactggaa agacaaggct gccgtgctga aggatgcgac accggccgcc gtgggttctc
     1141 gcgcggaagg gaagaacaaa tggggcgttt tcgatttgat cggaaatgtt tgggaatgga
     1201 cctcctcaaa agcctcggtg tacccgggca atccggccac agttcccagt agtattcaag
     1261 aatgggtaac gattcgaggc gggtgttatg tcagcgatcc cgcgaaatcc gacgcgccgg
     1321 tgtcgtcttg tttgcgtgag tttgttccgt cttcgacgaa gacgacgctg ctgggcttcc
     1381 ggctcgtgcg gagtggatcc tagccgcgga ttttcgcgaa tgaacgcgga tgatctacaa
     1441 tccactgcag tatttctcca gtgatccata agttctcttt ttgcaccagg gagagtaatg
     1501 atgagtatta acgcgattct gcgtgccgca tttttaagct cagccgcgct ttgcttgagc
     1561 ctgtgcacgg ttgcgagtgt tcatgggcag gacgtcggca tcgacccagg tggcagcgga
     1621 cttttccgtg ccaagaaccc tgagacgaag aagagtgcaa gtaaaccggg caccggggcg
     1681 aaacccgcaa accgaaattc tgccgcagcg cgcgccaaca ccgagcgtat agaagacctg
     1741 ctggaaaaag gcaacgaggc tcgtgacgcg cgaaaatttg cggacgctga aaccgcgtac
     1801 aaggaagttt tgaaactcaa accgcgcgac gcccgcgctg cctacggatt gggtaacatt
     1861 tattctgatc agcaacgctg ggacgatgcc gaagccgcgt accggaattc agtagcgtgg
     1921 gcgccgacta acgccgacgc ttatgtggcg ttgagcgtgg tgctggtgca gccgagctcc
     1981 ggtggtgaca acgccaggcg attggttgat gctgagtcgt tcgcaagacg atcggtgcag
     2041 atcgatccga agaatgcagt cggctgggat cgtcttggcg tagctttaca ggcgcgcggg
     2101 cttgcgaata gtgaaactga acattcgtat cgccgcgcga tcgagttgga tccgcagttc
     2161 gccgtggcct acgcacatct ggcgcgcatg ctcaatcgat ctggtcgcca ggctgagtcg
     2221 acgccgctgt atgagaaagc gatcgagctg gcaaaagatc cggccacgct caacctgatc
     2281 gccgactcgc tccagggtga gcagctttgg ccgcaatcgg agcctgtgtt aaaacgagcc
     2341 ctcgaactcg attcaagtaa tccgacgtcg ttggtgctga tgggacgtta tttgattgtc
     2401 tacaagagat accaggaggc cgaaccttac ttgaagtctg cgact
//
LOCUS       DASUEX010000648         2284 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1030677, whole genome shotgun sequence.
ACCESSION   DASUEX010000648 DASUEX010000000
VERSION     DASUEX010000648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1030677"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1021
                     /locus_tag="VFV61_10775"
     CDS             <1..1021
                     /locus_tag="VFV61_10775"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEX4901008.1"
                     /translation="ILSPRPFVPSLETSVKEKNLNLVRATRIPLIGRWWKSGLPRYLR
                     KNHFDLFHGTNFELPLTSDCATVLTIHDLSTLLLPHTHEGRNVARAKERLPGFAKAAT
                     MIVSPTDAIRQEIHEHLRIPLDKIVAIHEAARDCFQPLHFDETASIRKRFGVEGQFIL
                     YVGTVEPRKNLLRLVQAFEQCRREYEEPLQLVIAGRTGWMVNELYDYVRYSDAREAIV
                     FSGYLSDRELQELYSSCVLFLYPSHYEGFGLPPLEAMACGAPVIASNIDSIMEVTGSA
                     ARLVKPEVPDLKTAMLDLLMNWEARETLSRRGKQRAAQFSWSQTARRMREVYVEAIER
                     FRAGK"
     gene            complement(1085..2005)
                     /locus_tag="VFV61_10780"
     CDS             complement(1085..2005)
                     /locus_tag="VFV61_10780"
                     /inference="COORDINATES: protein motif:HMM:NF025314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4214 domain-containing protein"
                     /protein_id="HEX4901009.1"
                     /translation="MVKLTVLVLTIALAAGAAMGQAPTLRIVQTDGPDLPADLYYGNI
                     KVKPLRLRPGTNQVITIDDSDFFVSEQYVDFLGRFPDQGGFDYWTNQITSCGSNAACV
                     YSRRVGVSAAFFVEAEFQRTGSFVYRSFKGGLGRQPQYTEFGTDRRQVVEGPTLEQTK
                     QAYSLAFVQRAEFVAKYNGQNTAEAFVDALINTISPVNLNRAALINAYNTGADQNSSR
                     AKALRVAIDDPAFASAEYNKAFVLMQYFGYLRRDPDQGGYDFWLDVLNNREVNNYRGM
                     VCAFITSVEYQQRFGNTVTQNDHGCGNINL"
BASE COUNT          540 a          587 c          614 g          543 t
ORIGIN      
        1 cattctctct cctcgcccat ttgttccctc cctggagaca tccgttaagg aaaagaatct
       61 caacctggta agggccacga gaattcctct catcggtcgg tggtggaaat cgggccttcc
      121 tcgttacctg cgaaagaacc atttcgatct ctttcatggc acaaatttcg agcttcctct
      181 cacatctgat tgcgcaactg tgttgacgat ccacgatctt tcaacgctgc tccttccgca
      241 cactcatgaa ggtcgcaatg tggcccgtgc gaaagagcgg ttacccgggt ttgccaaagc
      301 tgcaaccatg attgtcagtc ccacggatgc aatccgacag gagatccacg agcatctccg
      361 gattccactg gataagatcg tggcgataca tgaggcagcg cgtgactgct tccaaccact
      421 tcattttgat gagacagcca gtatcagaaa gcgcttcggt gtggagggac aattcatctt
      481 gtatgtcggc acagttgagc cacgcaagaa tctgcttagg cttgtgcagg catttgagca
      541 atgccggcgc gagtatgagg aacccctgca gcttgtcatt gcggggcgga caggctggat
      601 ggtgaacgag ctctacgatt atgtacgcta ttctgatgct cgtgaggcga tagtttttag
      661 cggttacctg agcgaccggg aactgcagga gctatattcc tcatgcgtgc tcttcttgta
      721 tccatctcac tatgaggggt ttggtttgcc gccgcttgag gcgatggcgt gcggtgcacc
      781 ggttattgcg agcaacatcg acagcatcat ggaggtcaca ggctcagccg cgcgactagt
      841 caaacccgag gttcctgact taaagacggc gatgcttgat ctgttgatga actgggaggc
      901 aagagagacg ctctcccggc gaggtaagca acgcgctgcc cagttttcct ggagccaaac
      961 agcacgcagg atgcgagagg tctatgtcga agcgattgag agattccggg cagggaagta
     1021 aaaagagaga agggctagtt tctcagagaa accagccctt taggagcaaa caagttttgg
     1081 tgctttagag attgatatta ccgcagccgt gatcgttctg tgtaacggtg ttgccaaagc
     1141 gctgctgata ttccaccgac gtgatgaaag cgcataccat accgcgatag ttattcacct
     1201 cacggttatt caacacatca agccagaaat cgtaaccacc ctgatccggg tcgcgacgta
     1261 ggtaaccgaa atactgcatc aacacaaatg ccttattgta ttccgccgat gcaaaggccg
     1321 ggtcgtcgat ggctacccga agtgcctttg cgcgactgct attctggtct gcacccgtgt
     1381 tgtaagcatt gatgagcgcc gctcggttta ggttcacggg cgagatagta ttaatcagtg
     1441 catcgacgaa agcttcagcc gtgttctgcc cgttgtattt cgcgacgaat tcggctcgct
     1501 gaacaaacgc gagtgagtag gcctgctttg tctgttccag tgttgggcct tcaaccactt
     1561 gcctgcgatc ggtgccgaat tccgtgtact gcggctgccg gccaagacca cccttgaagc
     1621 tgcgatagac aaacgatcca gtacgctgaa attccgcttc cacgaagaaa gcggcactta
     1681 cgccgactcg tctgctatag acgcaggcag cgttgctgcc gcaactggtg atctgattgg
     1741 tccagtagtc aaagccgcct tgatcaggaa atcgtcccag gaaatcaacg tactgctcgc
     1801 tgacaaagaa atcggaatca tcgattgtga tcacctgatt tgtgccgggt cgcaagcgaa
     1861 ggggtttgac tttgatattg ccgtaataca agtctgctgg gagatcgggc ccgtcagtct
     1921 gcactattcg caaggtcggc gcctggccca tggccgcacc agccgctagg gcgatcgtca
     1981 gaacaagaac tgtcaactta accatcacta gactcctact attttcaggt tgcgaagact
     2041 gctgtaattg gatcaacgga aaagggaggt tctccgctgc aatcaaagaa gggccggggc
     2101 caattgccca tcaaaacaga gcgagtgtag cttgcgggaa acgtctttgt caacgaagtc
     2161 gtcttttcaa cttttctgtg gatcactggc aaaaattggc actcgccgcg atgctaaaca
     2221 acagcaggca ttaacgttgt tcgtttgcag ggttactcgc gcggcgacca tggattagga
     2281 cgtg
//
LOCUS       DASUEX010000649         3384 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1373340, whole genome shotgun sequence.
ACCESSION   DASUEX010000649 DASUEX010000000
VERSION     DASUEX010000649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3384
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1373340"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1365
                     /gene="acnA"
                     /locus_tag="VFV61_10785"
     CDS             <1..1365
                     /gene="acnA"
                     /locus_tag="VFV61_10785"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: protein motif:HMM:NF006757.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HEX4901010.1"
                     /translation="LAAGLLAKKAVELGLQVRPAVKNSLAPGSRVVTDYLARTGLQPY
                     LDKLGFNIVGYGCTTCIGNSGPLDPAVEQLVVDHDLIAASVLSGNRNFEARVHQSIKA
                     NFLMSPPLVVAFALAGRVDVDLTKDPLGKGKNGEPVYLRDVWPSMDEINNLLRSAFEP
                     ATYRQLYGNFADQNPLWNAIPTTTGNVYEWDAESTYIREPPYFENFSNEVGTWQDVHS
                     ARPLAIFGDSVTTDHISPAGAIKASSPAGLYLQERGVSIEDFNSYGARRGNHEVMVRG
                     TFANVRIRNLMVPGVEGGVTIHHPSGDRISIYEAAMRYQSEGVPLVVIAGQEYGTGSS
                     RDWAAKGTRLLGVKAVVAQSFERIHRSNLVGMGVLPCQFKEGANAASLRLDGTEQFEL
                     IGLEAGVKPLQDLKLVITRADGVRETVPVTLRIDTPIEIDYYQHGGILPFVLRQLLAG
                     TTDF"
     gene            1464..2972
                     /gene="betB"
                     /locus_tag="VFV61_10790"
     CDS             1464..2972
                     /gene="betB"
                     /locus_tag="VFV61_10790"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF009725.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008802 - betaine-aldehyde dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HEX4901011.1"
                     /translation="MSSVQPAETKTPRKYQLLIDGKWVDAESGKTFLTPNPATGETLA
                     EVAEADKADIDKAVVAARRAFEGKWSKISARDRGRMLYKLSQLIEENSAELAALETAD
                     NGKPIKESSFVDLPQVAENFEYFAGWATKIEGETIPVPGQMFNYTLREPVGVCGQIIP
                     WNFPLLMAAWKLAPALAAGNTVVLKPAEQTPVNAMELGKLIEQAGFPEGVVNIVPGYG
                     ETAGASLAAHPGIDKVAFTGSTEVGKLVAKAAADNLTKVSLELGGKAPNIVFADADLE
                     QAVNGAMMGIFFNQGQVCCAGSRLFVQEDVKEEFVDRLKEKSGKINVGDPMDKATHMG
                     PQVSEEQLNRIKGYVDIAQKEGATVVSGGVSPKLEGAFQKGYFFQPTIFSDVKNQMRV
                     AQEEIFGPVVSVIGFRDEDDLIKQANDTIYGLSAGLWTKDITRAHRFAKEIRAGVIWI
                     NTYNMFNAASPFGGYKQSGYGREMGKHALEMYTNVKSVWVDLSGKPIGWFGK"
     gene            3110..>3384
                     /locus_tag="VFV61_10795"
     CDS             3110..>3384
                     /locus_tag="VFV61_10795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4901012.1"
                     /translation="MHLAKRAGLGSLILIGALLLTACPKQETISRINADPGRYRNKEV
                     GIVGTVTDSYGVLGNGAYEIDDGTGRLWVVTTRGVPSRGSRVGTKGK"
BASE COUNT          813 a          869 c          982 g          720 t
ORIGIN      
        1 ctcgcggcgg gactgcttgc gaaaaaagcc gtcgagcttg gtttacaagt caggccagcc
       61 gtaaaaaatt ctctggcgcc ggggtcgcga gttgtgacgg actacctggc gcgtaccggc
      121 ctgcaaccat atctcgataa acttggcttc aacatcgtcg gctatggatg cacaacctgc
      181 atcggtaact cgggcccact cgatccggcg gtggaacagc tcgtggtgga ccatgatcta
      241 atcgccgcaa gcgtgctgtc tggcaatcgg aattttgagg cacgagtgca tcagagcatc
      301 aaggcaaact tcctgatgag cccgcccctt gtcgttgctt ttgcgctcgc tggccgtgtc
      361 gatgtcgatc taactaaaga cccgctcggc aagggtaaga atggtgaacc ggtttatctg
      421 cgcgacgttt ggcccagcat ggacgaaatc aacaatctgc tgcgctctgc ctttgaaccg
      481 gccacttatc gccagctcta cggaaacttt gccgaccaga atccgctttg gaatgctatt
      541 ccgacaacga ccggcaacgt ctacgagtgg gatgcggaat cgacttacat tcgtgagccg
      601 ccttactttg aaaacttcag caatgaggtt gggacctggc aagacgtgca cagtgctcga
      661 ccactcgcaa ttttcggaga ctctgtcacc acagatcaca ttagtccggc gggcgcaatc
      721 aaagcttcgt ctccggcggg tctgtacctg caagaaagag gcgtttccat tgaagacttc
      781 aacagctatg gcgcgcggcg cggcaatcat gaagtgatgg tgcgcggaac ttttgcgaat
      841 gtgcggatca gaaacttgat ggtgccggga gttgaaggcg gcgttacgat tcatcatccg
      901 agtggcgatc gcatcagcat ctatgaagcg gcgatgagat atcaaagcga aggcgtgccg
      961 ctcgtcgtga ttgccggaca ggagtatggc accggaagtt cgcgtgattg ggccgcgaag
     1021 ggaactcgac tgctcggcgt caaagctgtc gtggcccaga gtttcgagcg aattcaccgc
     1081 agcaacctgg tggggatggg ggttctgcca tgtcagttca aagaaggcgc taatgctgct
     1141 tcgctgcgac tggatggcac cgagcagttt gaattgattg gactggaagc aggtgtgaag
     1201 ccgttacagg atctgaagct cgtcattacg cgggcggatg gagtgaggga aacggttcca
     1261 gtcactctgc gcatcgatac cccaattgaa atcgactatt accagcatgg tgggattctt
     1321 ccgttcgtgc tgcgccagtt actcgcaggt acgaccgact tttaaggtgg cttgaggaca
     1381 aactaccgtt tgtcgttccc cgtttaccaa cgaagccaca tgcaatcagc tctacggtag
     1441 tagaaagaca gaggagcaaa acaatgtcat ccgttcaacc agccgaaacg aaaacaccgc
     1501 gcaagtatca acttctgatc gacggaaagt gggttgatgc tgaatcgggc aaaacattct
     1561 taacgccgaa tcccgccacc ggcgaaacgc tggccgaagt cgctgaagct gacaaagcag
     1621 acatcgataa agcagtagtt gctgctcgcc gggcttttga aggaaagtgg tcgaaaatca
     1681 gcgcccgcga tcgtggccgg atgctgtaca aactttcgca gttgattgaa gaaaacagcg
     1741 ccgagctcgc tgcgctggaa actgcggaca acggcaaacc gattaaggag tcgtcctttg
     1801 ttgacttgcc gcaggtagca gaaaacttcg agtactttgc tggctgggcc acaaaaatcg
     1861 aaggtgaaac gatcccggtc cccggacaga tgttcaatta cacgctgcgc gaaccagttg
     1921 gcgtctgcgg acaaatcatt ccctggaact ttccgctctt gatggccgcc tggaaactgg
     1981 ccccggccct ggcagccggc aacacagtcg tcctgaagcc cgccgaacaa actccggtca
     2041 acgctatgga attgggaaaa ctaatcgagc aagccggttt tcctgagggc gtggtgaata
     2101 tcgtaccggg ttacggtgag actgccggcg cgtctctcgc agctcatccg ggtatcgaca
     2161 aagttgcctt cactggttcc acggaagtcg gcaagctggt cgctaaagca gctgcggaca
     2221 atttgacgaa agtctctctc gagctgggcg gcaaggctcc caacatcgtc tttgcagatg
     2281 cagatcttga gcaggcagtt aatggcgcga tgatgggtat cttcttcaat caaggccaag
     2341 tctgctgcgc cggctcgcgg ctcttcgtgc aagaggatgt gaaagaagag ttcgtcgacc
     2401 ggctcaagga gaagtccgga aagatcaacg tgggcgatcc catggacaag gcgacccaca
     2461 tgggtccgca ggtttctgaa gagcagctta atcggatcaa aggctacgtg gatatcgcgc
     2521 aaaaagaagg cgccacggtg gtgagtggag gagtgtcgcc gaaacttgaa ggtgctttcc
     2581 agaagggata cttcttccag ccgacaattt tctctgacgt gaaaaaccaa atgcgtgtgg
     2641 cccaggaaga aatcttcggg ccggtggttt ctgtgattgg cttccgcgat gaagacgact
     2701 taatcaaaca ggccaacgac accatctatg gtctttcagc tgggctctgg acgaaggaca
     2761 tcacccgggc tcatcgcttc gccaaagaaa tcagggccgg tgtgatctgg atcaatacct
     2821 acaacatgtt taatgccgct tcgccgttcg gtggctataa acagtcgggc tacggcaggg
     2881 aaatgggcaa gcacgcttta gagatgtaca ccaacgtgaa gagtgtctgg gtggatctgt
     2941 cgggcaaacc cattggttgg tttgggaagt aaagtatttt gccggcgaga ctcgcggaaa
     3001 actgtttccg cgggtctcgc atctctgtaa cgccgctcct tcgttcgagc aaatgaggtc
     3061 cgaatgcggc gctctcccgc caggaaactc aacagaaaag gagacgcgta tgcatttagc
     3121 aaagcgggcg ggcttaggct cgctgatttt gatcggggca ctgcttctaa ctgcctgccc
     3181 aaaacaagaa accatttccc gaatcaacgc tgatcccggc cgctatcgca acaaagaagt
     3241 tggaattgtc ggcacggtca ccgatagtta tggcgtgttg gggaacggcg cctacgagat
     3301 cgatgatggc actggcaggc tctgggtcgt gactacccgg ggggttccgt cacgcggctc
     3361 aagagtgggc acgaaaggca agat
//
LOCUS       DASUEX010000650         3666 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_456316, whole genome shotgun sequence.
ACCESSION   DASUEX010000650 DASUEX010000000
VERSION     DASUEX010000650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3666
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456316"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1359
                     /locus_tag="VFV61_10800"
     CDS             <1..1359
                     /locus_tag="VFV61_10800"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEX4901013.1"
                     /translation="SKLDEHDLLGDTLIHRVAPRDVVEIGPFIIEFIRVSHSLVDCFS
                     LAIKTPVGTIIHTGDYKVDETPVIGEPIDLRSFRRYGQEGVLALLSDSTNATVPGRTP
                     SERAVIPAFEEIFAEAAGRIIVAAFASSIHRLQIVLDIAQQFDRKVCVLGRSMQKNVE
                     IAERLGYLDIPDGLLVPLNEAKQMRDNRIVFLVTGSQGESRAALSQMAVQSYKGMSIE
                     EGDTVVLSARIIPGNERVISRMIGFIYKRGANIIEEKRRLIHVSGHASQEDIRIMTEA
                     VRPKFVVPIHGEYRMLFRHKEFIKNHVGYKEEDIILIENGDVLELDGERAAIVNKRDI
                     GRTFIDDTGFEQIERETVRERKQMAAEGIVTLILTIDGETGELQATPEIVVRGVQGFD
                     GQNGSLKDAQRVIEAAVTGASRDTLADQSLLKEHVRVELKRFIQKLTGARPVIMPVVV
                     EV"
     gene            1511..2278
                     /locus_tag="VFV61_10805"
     CDS             1511..2278
                     /locus_tag="VFV61_10805"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX4901014.1"
                     /translation="MFWRNKTVMITGASSGIGRGLALELSKRGAKLGLVARRADKLAE
                     LTREIETANGKAIALPADVMDAASLATAATQLRTTFGPIDVLIANAGVGATVDGAELK
                     GSTVAGVINVNVLGAANSVEAVVPEMVKRGSGQLVVISSLAAYRGLPKSAAYCASKAA
                     VSAFFESLRLDLQPRGIDVTIIHPGFIKTPLTAGRHAQMPFLMELEDAVAEIISAIEK
                     RKKSYAFPWQLATIVRAGMIMPNFMYDWISRRNSFRE"
     gene            2418..3308
                     /gene="uppP"
                     /locus_tag="VFV61_10810"
                     /pseudo
     CDS             2418..3308
                     /gene="uppP"
                     /locus_tag="VFV61_10810"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphatase UppP"
     gene            3443..>3666
                     /locus_tag="VFV61_10815"
     CDS             3443..>3666
                     /locus_tag="VFV61_10815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4901015.1"
                     /translation="MSNNKGGELMNQNERRGASPSQRPRSPRVPVEFALEVEGNTPAG
                     ESFHVKAQAIKISRGGATIVLDREIAVGSVV"
BASE COUNT          858 a          925 c         1032 g          851 t
ORIGIN      
        1 agcaaactcg acgaacatga tctgcttggc gacacactga ttcaccgggt agcacctcgt
       61 gatgttgtcg agattggtcc gttcattatt gagtttattc gcgtctctca ttccctggtc
      121 gactgtttct cacttgctat caagaccccg gtgggcacga ttattcatac cggcgactac
      181 aaggtcgacg aaacccccgt aatcggtgaa ccgatcgatc tgcgttcctt ccgtcgctac
      241 ggacaggaag gagtgctggc gctgctatct gattcgacca atgcaaccgt gcccggtcgc
      301 acgccttccg agcgcgcagt cattcccgca tttgaagaaa tatttgccga ggctgcggga
      361 aggattattg tggccgcgtt tgcgtcctcg attcaccggc ttcagatcgt gctcgacata
      421 gcccaacagt ttgatcgcaa ggtgtgcgtg ctgggccgct cgatgcagaa gaacgttgag
      481 attgctgagc gccttggcta tctggacatc ccggacggtt tgttggttcc gctgaatgaa
      541 gcaaaacaga tgcgcgacaa ccggatcgtg tttctggtca cgggatcgca gggagagtcg
      601 cgggcggccc tctcgcaaat ggcggtgcaa agttacaaag gaatgtcgat cgaagaaggc
      661 gacacggtag tgttgtcggc gcgcatcatt cccggcaacg agcgggtaat ttcgcggatg
      721 attggcttca tttacaagcg aggcgccaac attatcgaag agaagcgccg cttgattcac
      781 gtgagcgggc atgcttccca ggaagacatc cgcatcatga cagaagcggt gcgaccaaaa
      841 tttgtggtgc cgattcacgg cgagtatcgc atgctcttcc gccacaagga gttcattaag
      901 aaccacgttg gttacaagga agaggacatt attttgatcg agaacggcga cgtgcttgag
      961 ctcgacggcg agcgcgcggc aatcgttaac aagcgggaca ttggccgcac gtttattgac
     1021 gataccggtt ttgagcagat cgagcgagag acggttcggg agcgcaagca aatggcagcc
     1081 gagggaatcg ttacgctgat cctgaccatt gatggagaaa ctggagagct gcaagcaaca
     1141 cccgagatcg ttgtccgcgg tgtgcagggc ttcgacggtc aaaatggatc tttaaaggat
     1201 gcgcagcgcg tgattgaagc tgcggttacc ggtgcttccc gagatacgct cgccgatcaa
     1261 agtttgctga aagagcacgt tcgcgtggag ttgaaacgtt tcattcaaaa attgaccggt
     1321 gcacggccgg tgataatgcc ggtggtcgtg gaagtctaat caaacaagag tctcaagtct
     1381 caggtctcaa gtcccaagtc tcaggtttca agcctcaaag tgtgagcgac atacttgagt
     1441 cttgagatcc ggagtctgaa acctgagact tgagtcctga gacttgagac ctgagactgc
     1501 gacttaaata atgttttggc gaaataagac agtgatgatc acgggagcgt cgtccgggat
     1561 cgggaggggt ctcgccctgg aactgtcgaa acgcggtgcg aagctgggtt tggttgcgcg
     1621 tcgcgccgac aaattggccg aactgacccg cgagattgaa accgcgaacg ggaaagcgat
     1681 cgcactgccc gccgatgtca tggacgccgc ttcactcgct acggccgcga ctcagttgcg
     1741 cacgactttt ggtccgatcg acgtgctgat tgctaacgcc ggagttggag ctaccgtcga
     1801 cggcgccgag ttgaagggaa gcaccgtggc gggcgtcatc aacgttaatg tcttgggcgc
     1861 ggccaacagc gtcgaggcag tcgtgccgga gatggtgaaa cgaggcagcg gtcaactggt
     1921 cgtgatttcc agtctcgccg cgtatcgcgg tttgccaaaa tcagcagcct actgcgccag
     1981 caaggcggct gtgtccgcat tttttgaaag cctgcgcctg gatctgcaac cgcgcgggat
     2041 tgatgtaacg atcatccatc cagggttcat taaaacccca ctgaccgcgg gcagacacgc
     2101 gcagatgcct ttcctgatgg agctggaaga tgcggtggca gaaatcatta gcgcaattga
     2161 gaaacgcaag aagtcgtatg cctttccctg gcagctcgct actattgtcc gggccggaat
     2221 gatcatgcca aacttcatgt acgattggat ttcacgccgg aattcgtttc gcgaataaga
     2281 tcgaactttg tactttgctc tttgtacttt gttcgttgtt caatcttgat gccagaattc
     2341 aaatggcata actaaaacaa agcagcaagt accaaataca aagttcaaaa gtgtcaagac
     2401 taaagctgac aatttagatg agcatatttc aagcgatcat cctcggcatc gttcaaggtc
     2461 ttaccgagtt catcccaatt agttcaacgg cacatcttgt ttttgcgtct cgtgcgattg
     2521 gtctttacag cggcgcgaac gcaaccctca aagctgaaca aaccacggcg acaatagccg
     2581 tgatccagtt aggaacattg cttgcagtcc tgatctattt ttcccgagac atcgtcagaa
     2641 tcagtcgtgc tttcatcgcc gatcacctca cgctgctgtc ggcgcgaaag tctcaaaatg
     2701 gggttggtgg gaaactctca caggacgcct ggctcggctg gctggtgata attggctcgc
     2761 tgccggtagg catcgttggc ctgctcttta aaaagcagat cgaaggcacg ttcacaaaaa
     2821 acctttgggt aattgcgacg atgatgatcg tggtggcaat ccttttaggg atcgccgaac
     2881 ttgtcgggaa gcgcacgcga tcgatgtaga gtttgggcat tcgagacgcc ttggcagttg
     2941 gcggcgctca ggtgttggcc ttgattccgg gatccagtcg atcgggctcg accattatgg
     3001 gcgggctgtt cgctggagag acacgcgaga ctgcggctcg attttctttt cttctttcca
     3061 tcccggccat cgctgcgagt ggcctgctgg aacttaaaga ggcgattgaa aaggtgccgt
     3121 cggagaatta cgttgctctc gcggtggcaa caatcgtctc cggagtcgtc ggctatgctt
     3181 caatttggtt tctgctacgg tttttgcgaa gccactcaac gggaattttt attggttatc
     3241 gcctaatcct gggggcaata atcatttttg cgctgcttgg cggttacctg tcggctgatc
     3301 tgagctaaga agcccaattc tggaactgag caaccgccct tccaaaaaca aaacgctaac
     3361 aataaatctc aagattagct aaactacact cgccccgact cctccccctc acgccggtgc
     3421 caaccggaac agtccaggaa taatgagtaa taacaagggc ggtgagttaa tgaatcagaa
     3481 tgagcgccga ggcgcgagtc cttcccagag gccgcgaagc cccagagtac ctgttgaatt
     3541 tgcgcttgaa gttgaaggca acacaccggc tggtgaatca ttccacgtca aggctcaggc
     3601 aataaaaatt agtcgtggcg gcgccacgat agtcctcgac cgcgagatag ccgtcggatc
     3661 tgtcgt
//
LOCUS       DASUEX010000651         2959 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_456460, whole genome shotgun sequence.
ACCESSION   DASUEX010000651 DASUEX010000000
VERSION     DASUEX010000651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2959
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456460"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="VFV61_10820"
     CDS             complement(<1..626)
                     /locus_tag="VFV61_10820"
                     /inference="COORDINATES: protein motif:HMM:TIGR01926.1"
                     /note="This protein belongs to a clade of uncharacterized
                     proteins related to peroxidases such as the
                     alkylhydroperoxidase AhpD; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase-related enzyme"
                     /protein_id="HEX4901016.1"
                     /translation="MSFLREVEVPDSFAPFVAFRESMGFVPKLFRAQTLLPRIIEAEA
                     QIAGSILFNERKLTRTQKECILLTLAASRRNTYCVTAHYHSLRTLGLKENQIERLIGG
                     HRGAGLPGADEALLDFALKLGRFPASLGQEDIAELRPFGFTDEGILEAVLMTALTNFL
                     CCLSTGLGVTSDFPPLQLGVDPGFKGPASHKGSGVVDLQGRGPYLSSV"
     gene            964..1674
                     /locus_tag="VFV61_10825"
     CDS             964..1674
                     /locus_tag="VFV61_10825"
                     /inference="COORDINATES: protein motif:HMM:TIGR02943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX4901017.1"
                     /translation="MKSERTSSPEERLLEPAMWLEAHGDYLFRHAMFRLRDINAAEDA
                     VQETLLAALQSHRAYEGRSSERTWLVGILKHKIIDHYRTISRTRDLAHENYQPQDEFD
                     PFEKNGEWAGHWRADWAPTAWHFNASTELDKKEFWATFDRCLSELPPKIAMAFTLREI
                     DGLSSEEICEVLNLSPNNLWVMLHRARLRLRHSLEAEWFGNKSRTSRNRPTAPGRPSS
                     ATEFPRLHRALKFVGLAA"
     gene            1805..2461
                     /locus_tag="VFV61_10830"
     CDS             1805..2461
                     /locus_tag="VFV61_10830"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEX4901018.1"
                     /translation="MKIVSLNIGRPRLVMRNGEPVSTAIFKEPVAGRVPLRTLNLDGD
                     RQADLSVHGGPTKAVYVYPSEHYDYWRQELPEMELPWGMFGENFTTEGLSEAQTNIGD
                     KFAVGSATVMVTEPRMPCYKLGIRFGRTDIIKRFLASGRSGFYLAVLQEGEVGVGDPL
                     ELIERHPSAIKVSDITRLYLDHGVDATLLRRAAELEPLPESWRTYFQDRLEKLSAASQ
                     "
     gene            2617..>2959
                     /locus_tag="VFV61_10835"
     CDS             2617..>2959
                     /locus_tag="VFV61_10835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4901019.1"
                     /translation="MIPNKLKSTVCVLLCTTILFTLPLQSSAQRTQKPVLHGKHWMAI
                     TGKPLAATAGAMTFQKGGNAVDAACAMIAATSTMWDTLSWGGETQALIYNPHTGKVIG
                     INALGVAPSGAT"
BASE COUNT          708 a          798 c          798 g          655 t
ORIGIN      
        1 tcgacagagc tcagataggg gccacgacct tgcaggtcta cgacgccgga gcctttatgg
       61 gacgccggcc ccttgaaacc tggatctact cctaactgca agggcgggaa atcggacgtc
      121 acgcccagac cagttgaaag gcagcagaga aagtttgtca gcgcagtcat taagacagct
      181 tccaatatgc cttcatctgt aaacccgaat gggcgtagct cggcaatatc ttcttgtccc
      241 agggaggccg gaaaacgacc gagtttaaga gcgaaatcca acaacgcctc gtctgctcct
      301 ggcagtccag ccccacgatg gccacctatg agccgttcta tttggttttc cttgaggccg
      361 agtgttcgca ggctgtgata gtgcgcagta acgcaatagg tgttgcgtct ggaggcagcg
      421 agcgtaagca ggatgcactc cttctgagtc cgggtaagtt tcctctcgtt gaaaagaatc
      481 gatccggcaa tctgcgcctc tgcttcgatg atgcgcggaa gtaaggtctg agcgcggaag
      541 agcttcggaa caaagcccat gctttcgcgg aaggccacga acggggcaaa cgaatccgga
      601 acttctactt cgcgcaagaa actcatagtt tggttccaac tacccggtga ttggaccaag
      661 aatatctcaa ttgtgcgatt agcggccaga gtagggtcga tagccttccc ctgcacggac
      721 tgagcacggc ctcccggcca tggctatttg atgacggtcc tgcggacctc acttggcatt
      781 ctcagacgtg tgtttgattt cgctgaaatt gagtcaaata tgccattgcg ccttgaagcc
      841 gggctgaaaa ggtgggcagc caaattttct gcatctcttt ctttccgcca ccgtctaaca
      901 aggtgaaggc gagtcagtct tactaaaaac cagcttcagc atccattgat atccagaaca
      961 gaaatgaagt ccgaacgaac aagttctcca gaggagcgac tgcttgagcc agccatgtgg
     1021 ctggaagcgc acggcgatta tctcttccgg catgcgatgt tccggctgcg cgacatcaat
     1081 gccgccgagg atgccgttca ggaaacgctg ctggctgcat tgcaatctca tcgcgcctat
     1141 gagggtcgta gctccgagcg aacgtggctg gtcggaattc taaaacacaa gatcatcgac
     1201 cactaccgca ctatcagcag aactcgcgac cttgctcatg agaactatca gccacaggac
     1261 gagttcgatc cttttgaaaa gaatggcgag tgggccggtc attggcgcgc tgactgggcg
     1321 ccaaccgcat ggcatttcaa cgcctccacc gagttggata agaaagaatt ctgggcaacc
     1381 tttgaccggt gtttgtcgga gttgccgccc aagatcgcga tggcctttac gctaagagag
     1441 atcgatggtt tgagctccga agaaatttgc gaggttctta atctctcacc caacaacctc
     1501 tgggtgatgc ttcaccgtgc gcgactgcgg cttaggcatt cgctggaagc cgaatggttt
     1561 ggcaacaaat cgcggacttc gcgaaacaga ccaaccgcgc ctgggcgacc aagtagcgcg
     1621 acagagtttc cccgacttca tcgcgccctt aagtttgttg gcctcgctgc ttgaaagttg
     1681 ccctccactg ctttatcact tgccattaga gaatccccta acactgccta cgcttttggg
     1741 gtatggcaac ttgcgccgta ttttaagcgc ggacaggcgg cgcattccaa agaggttttc
     1801 agtcatgaaa atagtatctc ttaatatcgg tagaccccgg ctggtaatgc gcaacggcga
     1861 gccggtttct acggcaatct tcaaagaacc tgtagccggt cgcgtgccgt tgcgaacgtt
     1921 gaacctcgac ggcgaccggc aggctgatct atcagttcat ggcggaccaa ccaaggccgt
     1981 ctacgtctac ccatccgaac attacgacta ctggagacag gaacttccgg agatggagct
     2041 gccttggggc atgtttgggg aaaactttac gacggaggga ctctcggaag cccaaacaaa
     2101 catcggcgac aagtttgcgg tcggctccgc caccgtgatg gtgactgaac cgcgcatgcc
     2161 ttgctacaaa ctcggtattc gctttggccg gacagacatc atcaagcgat tcctggccag
     2221 tgggcgcagc ggattctatc tggcggtatt gcaggagggc gaggttgggg tcggtgatcc
     2281 gctagagcta atcgaaaggc acccgagcgc tatcaaggtc agcgatatca cgcgcctata
     2341 tcttgaccat ggtgtcgacg ccacactgct gcgtcgggct gcagaattag aacctcttcc
     2401 ggaaagctgg cgaacttatt ttcaagaccg gctggagaag ctttctgcag cgtcccaata
     2461 gcggatcaga tagcgaaaca ctagcgagta cgattaacag ggaaggtttt tttggtgtta
     2521 gattacccac ctgcaaacgg ctgaggacat cagcaataac tacctccttc gctcggcggt
     2581 tcacaatgtg gttggaatct ctaaggaaag agtcagatga taccgaacaa gcttaaatca
     2641 acagtttgtg tccttctttg cacaaccatc ctttttaccc ttcccctgca atcgagtgcg
     2701 cagcgaaccc aaaaacctgt gctgcatggc aagcattgga tggcgattac cggtaaaccc
     2761 ctggcggcaa ctgccggggc gatgaccttc cagaagggcg gcaacgctgt cgatgcagcc
     2821 tgcgcaatga tcgccgctac ctcaacgatg tgggacacac tctcctgggg tggcgaaacg
     2881 caagcactaa tttataaccc acacacagga aaggtcatcg gtattaatgc tttgggcgtg
     2941 gctccatcag gcgcaaccg
//
LOCUS       DASUEX010000652         2838 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1258479, whole genome shotgun sequence.
ACCESSION   DASUEX010000652 DASUEX010000000
VERSION     DASUEX010000652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2838
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1258479"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            27..1157
                     /locus_tag="VFV61_10840"
     CDS             27..1157
                     /locus_tag="VFV61_10840"
                     /inference="COORDINATES: protein motif:HMM:TIGR02177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HEX4901020.1"
                     /translation="MMTTSGSNGQNGSGNGNKPIVETAVSAESDDGAVSFKVAPPPGE
                     LKKADFVSDQEVRWCPGCGDYAILNNVQKVMPELGVPREKIVFVSGIGCSSRFPYYMN
                     TYGFHSIHGRAPAIATGIKCANPELSVWVITGDGDALSIGGNHFMHAIRRNLDLNYIL
                     FNNRIYGLTKGQYSPTSEFGKRTKSSPIGAIDYPVNPLSLAIASEATFVGRSIDIDVK
                     HLGSVVEAAARHKGISFVEVYQNCNIFNDHAFEQFAERSVRQDRVLYLEHGKPMIFGK
                     EHNRGIRMKGALPEVVTIGENGITEADLLVHDIYLEDPSVAFMLARMEQPEFPQPVGI
                     FRAVERATYEDLMTNQISEAIAKSGPGNLDKLIHSGDTWVVE"
     gene            complement(1312..2511)
                     /locus_tag="VFV61_10845"
     CDS             complement(1312..2511)
                     /locus_tag="VFV61_10845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4901021.1"
                     /translation="MSTTTKNPVVEAIISGKAPQPARLAAASGLLPLPQADLLEVLVA
                     LQQQDDPEIATAAGETLREQDNSDLLNAAKNTDTSAAVLSYLGSVTGAREIHEAVILN
                     KNTPDQAIVQLASTIEDGSLLELITQNQQRLVRLPKIIDAVLQNPARTTEAERRARET
                     QTEFFEKERGAKQIAGELRARGKNAAAEFFESAELTTIHGELSLEDAWLIAEHIEVSD
                     AELDDSWLPSERYDEVEDEASLAAAMQKVIEYETLEAGAPIAPDRVSLIRRLMLMNAR
                     DRMKLARKGDREARSILIRDSNKMVAVAVINNPRITEQEAENIATMRTVADEVLRLMA
                     MNRNWARSYTIIHNLVRNPRTPIPTVINILPRIRTKDLLGLKQNRNISDAIRRQANRL
                     AQARSGE"
     gene            complement(2619..>2838)
                     /locus_tag="VFV61_10850"
     CDS             complement(2619..>2838)
                     /locus_tag="VFV61_10850"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEX4901022.1"
                     /translation="EARQAGVRGTVVLRGILAADGTVQNLLVIKGLPGGLSWQAVEAA
                     RQIRFTPAMIDGKPVSMFVQMEYCFDLY"
BASE COUNT          629 a          736 c          789 g          684 t
ORIGIN      
        1 aacacgcaag ataaaactga ggagcaatga tgactacaag cggttctaac ggacaaaacg
       61 gaagtggcaa cggtaacaag ccaatcgttg agacagcagt atctgccgag agtgatgatg
      121 gagctgtttc cttcaaggta gccccaccac ccggcgagtt aaagaaagcc gattttgttt
      181 ctgatcagga agtgcgctgg tgccctggct gtggtgacta cgcaatcctc aataacgtgc
      241 agaaggtgat gcccgagctt ggggtgccgc gcgaaaaaat tgtcttcgtg tctggtatcg
      301 gctgctcgtc gcgctttcct tactacatga acacctacgg ctttcacagt attcatggac
      361 gcgcgccggc aatcgccacg ggcatcaagt gcgcgaaccc tgagttgagt gtctgggtga
      421 ttaccggtga cggcgatgcg ctctcgattg gcggcaatca cttcatgcat gcgatccggc
      481 gcaatctcga tctcaattac atccttttca acaatcgcat ctacgggctg acaaaggggc
      541 aatacagtcc tacgtcagag tttggcaagc ggaccaaatc atcgccaatt ggggcgattg
      601 attatccggt gaaccctttg tcgctcgcga ttgccagcga ggcaaccttt gtgggacgct
      661 caattgatat cgatgtgaag catctcggga gtgtggtcga agctgcggcc agacacaaag
      721 gaatctcgtt cgtggaggtt tatcagaact gcaacatctt caatgaccac gccttcgagc
      781 aatttgccga acgcagcgtc cgccaggacc gtgtgcttta cctggaacac ggcaagccaa
      841 tgatctttgg taaagagcac aatcgcggca ttcgcatgaa gggcgcgcta ccggaagtgg
      901 taaccattgg cgaaaacgga atcactgaag ccgacttgct ggtgcacgat atttatcttg
      961 aagacccgtc ggtcgctttc atgctcgcac gcatggagca gccggagttc ccgcaaccgg
     1021 tgggcatctt ccgcgccgtg gagcgtgcta cttatgagga tttgatgact aatcaaatca
     1081 gcgaggcgat tgcgaagagc gggccgggaa atctcgacaa gctgattcac agcggcgaca
     1141 cctgggttgt cgaataaaaa cgtccaaggt ctaaagtcca acgtccaacg tccaaggtcc
     1201 gcttccgaag cggacctttt tctttttgat aacaggcttc aaacgtaagc gatccagatc
     1261 ttaaatttgc ggttgaacct tggacttttg gacgttcgac tttggacgtt cttattctcc
     1321 cgaacgcgct tgggcgaggc ggttggcttg acggcggatg gcgtcggaaa tattccggtt
     1381 ctgtttgagg cctagcagat ccttggtgcg gatccgcggc aggatattga tgacagtggg
     1441 aatcggagtt cgaggattac gcaccaggtt atgaatgatg gtgtaagaac gcgcccagtt
     1501 tcggttcatt gccatcagac gcaacacttc atcggcaaca gtgcgcatcg ttgcgatgtt
     1561 ttccgcctcc tgctctgtga tgcgcggatt gttgatgact gcgacggcaa ccattttgtt
     1621 tgaatcgcga atcaagatgc tgcgtgcctc gcgatcgccc ttgcgtgcca gcttcatccg
     1681 gtcgcgtgcg ttcatcaaca tcaaacgtcg aatcaaggag acgcgatcgg gcgcaatagg
     1741 cgcccccgct tctaacgttt cgtattcgat taccttctgc atggcagccg cgaggctggc
     1801 ttcgtcttct acttcatcat aacgttcgga aggtagccag gaatcatcga gttcagcatc
     1861 agagacttcg atgtgctcgg caatcagcca tgcgtcttcc aaactgagtt cgccatggat
     1921 ggtcgtcagc tcggcagact caaaaaactc tgctgcggcg ttcttgccgc gggcgcgcaa
     1981 ctcgccggca atttgtttgg cgccgcgttc cttctcgaag aattcagtct gcgtttcccg
     2041 cgcgcgtcgt tcagcttcag tcgtgcgcgc tggattctgc agcaccgcgt cgatgatctt
     2101 gggcaggcgc accagccgtt gctggttttg cgtgatcaat tccagcagcg aaccatcctc
     2161 aatcgtcgag gcaagctgca cgatcgcctg gtccggcgtg ttcttgttca gaattacagc
     2221 ttcatgaatt tcacgcgcgc ccgttacgct gccgagataa ctaagcactg cggcagaggt
     2281 gtcggtgttt tttgcggcgt ttaagagatc agagttgtcc tgctcccgca gtgtttctcc
     2341 ggccgccgtt gctatttcgg ggtcgtcttg ttgttgcagc gccaccagca cttcaagaag
     2401 atctgcttgt ggaagaggta agagtccact ggccgccgcc agccgcgccg gttgcggagc
     2461 tttgccgctg atgatggctt caacaactgg gtttttagtg gtagtactca tgaaactgag
     2521 tgacgcagat cgagtttacc ttaaacggga cgagagtggt ggtgagtttg aaaggaaaga
     2581 aaagcgggag gataaggcga ttgtacgtta agacctcatc agtagaggtc aaagcagtat
     2641 tccatctgga caaacattga gaccggttta ccgtcgatca tcgcaggtgt gaaccttatt
     2701 tgccgtgctg cttctacggc ttgccaacta agacctccag gcaggccttt aattaccaaa
     2761 agattttgga ccgtcccgtc cgcggccagg attccgcgta atacaactgt acccctgacg
     2821 ccagcctggc gggcttcg
//
LOCUS       DASUEX010000653         3445 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_668344, whole genome shotgun sequence.
ACCESSION   DASUEX010000653 DASUEX010000000
VERSION     DASUEX010000653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3445
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_668344"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(113..265)
                     /locus_tag="VFV61_10855"
     CDS             complement(113..265)
                     /locus_tag="VFV61_10855"
                     /inference="COORDINATES: protein motif:HMM:NF033488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HEX4901023.1"
                     /translation="MLWTIFVVLLVLWLIGMVSSYTMGGFIHLLLLIALVMLIINLIS
                     GRRPVV"
     gene            771..2573
                     /gene="ftsH"
                     /locus_tag="VFV61_10860"
     CDS             771..2573
                     /gene="ftsH"
                     /locus_tag="VFV61_10860"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEX4901024.1"
                     /translation="MPSPIRKTVFSSQKVPVVLAAAFALALLATTVVLIIQSRAPQVT
                     SINYSQLYQVAETGAALSLTIEGDQLTVQSNQGTALQASVTSEIARQGLVEQFRKNNV
                     PIEFRQPQLTWGATILTWIAPFATVLLLGLIGWRVHASMSGGGTGNFSVTNQNGKQDV
                     SFADVAGVDEATSELAETIDFLRNPERFGRLGGRAPRGILLYGPPGTGKTLLARAAAG
                     EAGVPFLLASGSSFQEKFAGVGAARVRRLFAEGKKLSPCIIFIDEIDALGRQRGRGND
                     SATADQDQTLNQLLIEMDGFDQSSSIVVMGSTNRPDVLDRALTRPGRFDREIAVNLPD
                     ARGREAVLNVHARRLELDSELDLGWVARGTPGFSGADLANLLNEAAIAATRDDSTAVG
                     RTHIEYARDRILMGAERHGFMVDEDERYATAVHEAGHVAVGLAVKNGDPIHKVSILPR
                     GRALGVTQSLPERDRLMKKREYLEDQIAVLLGGRAAEQILLNTMTAGASNDIQRAVEI
                     ARSMVSEFGMSPLGPIYVSDPRGAAAAPLSQSLLDRVEEAVNDIINTQLAKACEVVTA
                     SQESIGRLVELLLEQETVEADGIEECFMAQRHAA"
     gene            complement(2995..>3445)
                     /locus_tag="VFV61_10865"
     CDS             complement(2995..>3445)
                     /locus_tag="VFV61_10865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4901025.1"
                     /translation="PEKQAAYQVSFSGLAAGGHVLELRNMRGAVYVDGFCMESSSSSA
                     QPSAGPGTTSSNTTSLNLGKQLLQSLTLPAGTQAISVVAEASPELPIQLLLIDPSGAV
                     LNTANNSTGIATINRTVSGSGIYTIKVVNVSVGSVNVWTAATPHVQR"
BASE COUNT          727 a          967 c          983 g          768 t
ORIGIN      
        1 ttgttgtatt agttagtgga tcgctcaatt cccgcagccg agagagcggg ggcaagcccg
       61 ccttccgaac tgcaattcta attggcaatc ggcaattggc aatttcccaa gcttaaacca
      121 ctggcctacg accgctgatc aggttgatga ttagcatcac gagtgcaatg agcaggagca
      181 ggtgaataaa accacccatc gtgtagctgc tgaccattcc gattaaccac aacaccagta
      241 gaactacgaa gatcgtccaa agcatttttc ggttcctcct ttaatactag ctacgatcaa
      301 gttcccaatg cgttggtaat cgagttggca aagcgtatgc cgcctctctg atccgaagcg
      361 gcaaactgtt gattgaacgt gacttttctg tagtaactac agccttggat gtcatcgcgc
      421 ccggccctcg tgcgtactaa aacccgccag taaaggtacg gagcgggtga cgcggggacg
      481 cggagacgcg gagacgcggg gacacggaga cgcggagacg cgtctttttt taattctgga
      541 cttatcagtt tgacggctgg taaagtaaag ccgtgctcgc gcctccccat ctagcgagcg
      601 cgatcacgtt aaaagagctc gggttttgca ctgcgccgtt tgtcccccga cactccgtcg
      661 ccccaatcct gatccaattg aaatttcgcg acagcacgca cgcgcttgtc gcttagtgag
      721 cgctcgagaa ccccgacacc tggaggcagc gagcgtcttt ggagtctgtt atgccttccc
      781 ccattaggaa aaccgttttc agctctcaaa aagtgcccgt tgttttggcc gctgcctttg
      841 ctcttgctct ccttgcaaca actgtcgtgc tcatcatcca gagccgtgca ccgcaggtca
      901 ccagcatcaa ctattctcag ctctatcagg ttgcggaaac cggagctgcc ctgtcgctga
      961 ctattgaagg cgatcagcta acggtgcaga gcaaccaggg aactgctctg caggccagtg
     1021 tcacgagtga gatagctcgt cagggtctgg tcgaacagtt tcggaaaaat aatgttccga
     1081 ttgagtttcg ccaacctcag ttgacctggg gcgcgaccat ccttacctgg atcgctccgt
     1141 tcgcgacggt gctgttgctc ggactgatcg gctggcgagt tcatgccagc atgagtggcg
     1201 gtggcaccgg caacttcagc gtcacaaatc aaaatgggaa acaagacgtt tcgtttgctg
     1261 atgtcgccgg cgttgacgag gccacttcag agttagctga gacgatcgac ttcctgcgta
     1321 acccggagcg ctttggccgc ctgggtggac gggccccgcg gggaattttg ctttatgggc
     1381 cgccgggtac cggcaagact ctgttagcgc gggccgcggc cggcgaagcg ggcgtgccct
     1441 ttttgctcgc atcaggttcc agcttccagg aaaagtttgc cggtgtgggc gctgcgcgtg
     1501 tgcgccggct cttcgccgag ggcaagaaac tttcaccctg catcatcttc atcgatgaga
     1561 ttgacgcgtt gggaagacaa cgtggtcgcg gtaacgattc agccactgcc gatcaggatc
     1621 aaacgctgaa ccaactgctg attgaaatgg atggctttga tcagagttcg agcatcgtgg
     1681 tgatgggatc gaccaaccgg cctgatgttt tggatcgcgc cctcacacgt cccggtcgct
     1741 tcgatcgcga aatcgcagtc aacctgcccg acgcgcgtgg tcgtgaggcg gttttgaacg
     1801 tgcacgcgcg ccgtttggaa ctcgatagcg agttggacct cggctgggtg gctcgcggca
     1861 ctccgggttt ctccggcgcc gatctggcga accttctgaa tgaagcagcg attgcggcaa
     1921 cgcgcgatga ctccaccgcc gtggggcgca cgcacatcga atacgctcgc gatcggattc
     1981 tgatgggagc ggaacgtcat ggattcatgg tcgacgaaga cgagcgctat gcaacggcgg
     2041 ttcacgaagc cggccacgtc gcggtcggcc tggcggttaa gaatggcgac ccgattcaca
     2101 aggtttctat cctgcctcgc ggacgtgctc tcggcgtcac gcagtctttg ccggaacgcg
     2161 atcgcttgat gaagaagcgc gaatatctcg aggatcaaat cgccgtactc ttaggcggac
     2221 gtgctgccga acagattctg ctgaatacga tgacagccgg cgcttcgaac gatattcagc
     2281 gcgcggtaga gatcgcacgc agcatggtga gcgagtttgg tatgagtccg ctcgggccga
     2341 tttacgttag cgatccccgc ggtgctgccg ccgcgccgtt gagtcagagc ctgcttgatc
     2401 gagttgagga agcggttaac gacatcatca acacgcaatt ggcgaaggcc tgcgaggttg
     2461 taacggcaag ccaggagtcg attggccgcc tggttgaatt actgctcgag caggaaacgg
     2521 tggaagccga tggcatcgag gaatgcttta tggctcagcg tcacgcggct taggtgatac
     2581 cgccaaagcg ccaagaaacg ccaaaggctc caaggaatta ggaaattgag cccgctttag
     2641 tgggcgaccg cgtaaagcct ggggcgtgag ccccaggttc aagcccgcaa aaaatccact
     2701 aagcccgcgg agcgggcgac agagctttat tgaaggactg tcgcccgctt cgcgggctct
     2761 ttcctttctc tcaatcttac ctggggttcc gctgcgctcc accccaggct ttatgctttt
     2821 acccgcttcg cgggcaggtt gaaatcctac ttgctgcgtt gcaaaaagtt ccgccaaggg
     2881 cgccaagaat tctagagctg caaagttgaa ttagatttct gactcaactc gaccgcttaa
     2941 gaactcttgg cgcccttggc gtttcttggc gccttggtga atgcttaggt gcctctacct
     3001 ctgcacgtgt ggtgtcgccg cagtccagac attcacggaa ccaacactca cgttcactac
     3061 cttgattgta tagattccgg atccgctaac ggtcctgtta atcgtcgcaa tccccgtcga
     3121 gttgttggct gtattcaaca ccgcgccgga aggatcgatc agcaagagct gaatcggtaa
     3181 ctccggactg gcctcagcca ccactgagat cgcctgcgtg ccggcaggta aggtcaggct
     3241 ttgtagcaac tgcttgccaa gattcaacga agtagtgttg ctactggttg ttcccggacc
     3301 tgcactcggt tgcgcgcttg aactcgagct ctccatgcag aagccgtcca catagactgc
     3361 accacgcatg ttgcgcagct cgagaacatg gccccctgcc gccaggccgc taaacgaaac
     3421 ctgataagcg gcttgcttct cgggc
//
LOCUS       DASUEX010000654         4844 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_332641, whole genome shotgun sequence.
ACCESSION   DASUEX010000654 DASUEX010000000
VERSION     DASUEX010000654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4844
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332641"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1088
                     /locus_tag="VFV61_10870"
     CDS             <1..1088
                     /locus_tag="VFV61_10870"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX4901026.1"
                     /translation="PALGQAGRRPEPSPTPPNQDQEPVRVFTEEVRLPVIALDQFGHY
                     DPTLEPDDVLVLEDGVAQQVRSVRHIPANVLLLLDTGGELSGLGGSSKLTSLTRDVAI
                     QVLQSVPDGTSLAVMQFNNSVELLQPWTADKTLLLKVLKTKLSSGKRARFSDALVAAA
                     SLMKDLPEGSRHLVVITDGVDTAGTKTSRAEAIKQLAAARATVHIISYTEFVLQKKEG
                     RTPGVSSGQVATARDPITATDPTLPPGTTRSPSFGVGIRFDPAMKRQRKAYEGEIKKS
                     QQVLTSVANEMGGRIFLPLNSEEMLAQAREAASEIGSEYVITYRPKRPLANSRPGEYR
                     RLEVAPRKQGLTLRARRGYVVPTGRND"
     gene            complement(1095..2225)
                     /locus_tag="VFV61_10875"
     CDS             complement(1095..2225)
                     /locus_tag="VFV61_10875"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX4901027.1"
                     /translation="MPGDAKSRILIVDDEPDITEILADLLGDNHECTTANSAEAALEL
                     LAANEFHLVVSDITMPGMSGLEMLPRAKSISPNTVVVMISGMQTVESAIGALRLGGFD
                     YLMKPFDLRQVEAVVKRALEHHELIVAKQRYENHLEELVEQRTAELDQALNSLEDAYR
                     STLKALTAALETRDSETHGHSERVVTYSLRLGREYGLSAEQMKSLEFGSLLHDIGKIG
                     VPDLILRKPAKLTEEEWVLMREHPLHGQQILRGIEFLAGPARVVAQHHEKWDGSGYPL
                     GLQGHDIDLCARIFSVADAFDAITSNRVYRRGKSYQEAADELDLWAGRQFDPKVVEAF
                     HRVPPQDWDELHKRSLQPKPEDHDVRRVVQSLESQLELMATP"
     gene            complement(2559..2891)
                     /locus_tag="VFV61_10880"
     CDS             complement(2559..2891)
                     /locus_tag="VFV61_10880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4901028.1"
                     /translation="MRKLLATLVVTTLLLLMAVPASAQCGRYRHRHSNVTHTHYSCIG
                     DHHKHGRSFWQKHRDKLTTAGGVGAGALIGGIAGGKKGALIGGAVGGGGAAVYTYGIR
                     KRRRSARY"
     gene            3107..3193
                     /locus_tag="VFV61_10885"
     tRNA            3107..3193
                     /locus_tag="VFV61_10885"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3141..3143,aa:Leu,seq:caa)
     gene            3212..4609
                     /locus_tag="VFV61_10890"
     CDS             3212..4609
                     /locus_tag="VFV61_10890"
                     /EC_number="3.5.3.13"
                     /inference="COORDINATES: protein motif:HMM:NF006681.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamate deiminase"
                     /protein_id="HEX4901029.1"
                     /translation="MNHSPQAWLPDLVYTGKEFKNNLTLATDSTGRVLRLASAREDEA
                     VVRLKHCAVLPGLVNAHSHAFQRVIRGRTEHRTTGTTDSFWTWREMMYSAAARLTPED
                     LYDASRMAFMEMALSGITTVGEFHYLHNQADGSSYDDPNLLAKEVVRAARDVGLRIAL
                     LRVAYARAGYQVDPNPRQSRFIESTSDVYLKNLEQLRSELASMAGEAWVGVAPHSVRA
                     VPLDQLKTIVAHANQAQLPVHMHVAEQPAEVSACIAEHGRSPVALLESEGLLSERFTG
                     VHVIHITAKAARMIAAARAMVCACPSTERNLGDGVVPMDLLLTANVDVSLGTDSQAQI
                     DLLEDARELEYHLRLQKLERAVLSLPSDEDQAALAVRLFDCATINGARSLGVAGQPLA
                     NGRVADFFTVDLDDPSIAGATRDTLLTTIVFSLSKSAVKDVVVGGKRIVEAGRHFAQE
                     EITERFRELQKRLWS"
BASE COUNT         1060 a         1353 c         1359 g         1072 t
ORIGIN      
        1 tccctgcctt gggtcaggca gggcggcggc ctgaaccgtc acccactccg cctaatcaag
       61 accaagaacc agttcgagta tttacggagg aagttcgttt gcccgtaatc gccctcgatc
      121 aatttggaca ctatgatccg acgctcgagc ccgatgacgt tttggtgctc gaagatggtg
      181 tggcccagca agtgcggagc gttcgtcata tccccgccaa tgttttgttg ttactcgata
      241 ccggcggtga gttgagcgga cttggtgggt cttcaaaact tacatcgcta acacgtgacg
      301 ttgcgattca agtactgcaa agtgtgcctg acggtacctc gctcgcggtg atgcagttca
      361 acaactcggt tgagttgctg caaccctgga ctgccgacaa gacgctgttg ttgaaagtct
      421 tgaaaaccaa attatcctcc ggcaagcgcg cgcgcttttc agatgcgctg gttgcggcgg
      481 cgagtctgat gaaagacctt cccgagggaa gccggcatct tgtcgtaatt acagatgggg
      541 ttgataccgc cggaacgaaa accagccggg cggaggcgat caaacaactt gctgccgcgc
      601 gcgctacggt gcacatcatt agctacacag aattcgtttt gcagaagaaa gaaggcagaa
      661 ctcccggcgt ctcatccgga caagttgcaa ctgcgagaga tcccataact gcaactgacc
      721 ctaccctacc tccgggcact acgcggagtc cctcctttgg agttggcatc cgtttcgacc
      781 cggcaatgaa gcgacagcgc aaggcgtatg aaggcgagat caaaaaaagc cagcaggtgc
      841 tgacttcagt ggccaatgag atgggtggtc gaatttttct gccgttgaat agcgaagaga
      901 tgttggctca ggccagggaa gctgccagcg aaataggttc tgagtatgtg attacctatc
      961 ggcccaaacg ccctttggcc aactcccgac cgggtgagta tcgtcgcctg gaagtggctc
     1021 cgcgcaagca gggactaact ttgcgagccc ggcgtggcta cgtggtgccg accggccgca
     1081 acgattgatt taagctaagg cgtagccatt agttctagtt gcgattcgag cgactgcacc
     1141 acgcgacgta catcgtgatc ttcgggtttt ggttgcaaag aacgcttgtg tagttcgtcc
     1201 cagtcctgcg gcggcacacg atgaaaggct tcaaccactt tcggatcaaa ttgccggccc
     1261 gcccacaggt ccaactcatc agcagcttcc tgatacgact taccgcgacg atagactcga
     1321 ttgctggtga tcgcgtcgaa agcatcggct accgaaaaga tgcgtgcgca caagtcgatg
     1381 tcgtggcctt gtagtcccag gggatatccc gaaccgtccc acttttcatg atgctgggct
     1441 acgactcgcg ccggtcctgc cagaaactca atgccacgca agatctgctg tccgtgaagc
     1501 gggtgttccc gcatcagcac ccattcctcc tcggtcagtt ttgccggttt acgcagaatc
     1561 aagtcaggca ctccgatctt tccgatgtcg tgcaggagcg aaccaaactc cagcgacttc
     1621 atttgctccg cgctaagtcc atattcgcgg cccaggcgca ggctgtaggt aacaacacgt
     1681 tcggaatggc catgcgtctc cgagtctctc gtctccagcg cggccgtcaa tgccttcaaa
     1741 gttgaacgat aagcgtcctc caacgaattc aacgcctgat cgagttcagc cgtccgttgt
     1801 tcgacgagct cttccaggtg attctcataa cgctgcttgg caacaatgag ttcatgatgc
     1861 tccagagcgc gtttgaccac tgcctctacc tgacgcaagt caaaaggctt cattaaataa
     1921 tcgaagccgc ccaggcgcag cgcgccgatt gcgctctcta ctgtctgcat gccgctgatc
     1981 atcacaacga ccgtgttcgg cgagatgctc ttcgcgcgtg gcaacatttc caggccactc
     2041 attccgggca tggtgatatc gctcaccacg agatgaaact cgttcgcggc cagcagttca
     2101 agagcggctt cggcagagtt ggcggtggtg cattcgtgat tgtcgccgag caagtcagcg
     2161 agaatctctg ttatgtcggg ttcgtcatca acgatgagaa tccgtgactt ggcgtcacct
     2221 ggcatcaatg tggctcctgg atgttagtag gtttcgcatt gcggcgaccg gacaaactgg
     2281 cagctatcgc gtcgccatta atcgaggttt ttttggggtc gcaggggcgg tgtatcgcgg
     2341 ataaaactac ctgcattttg gcaggttttt ggattctaca gtgttcggcc ctgtgtgcca
     2401 acaagttttt gccggaactt gagacgttgg cttcggacga gacggcgggc taacggggag
     2461 ccgaatatca caagaaaacc tagcattaaa cgcagaaacg cccgtcccga ggtaacggga
     2521 agggcagttt ctcgcggatt cgaactccga gcaggacctt aatatctggc tgaacggcgt
     2581 cgtttgcgga ttccgtaggt atacacggct gcaccgccac cacccaccgc gccgcctatc
     2641 agcgcgccct ttttgccccc ggcaataccg ccaatgaggg cgccggcccc aaccccgccc
     2701 gcggtcgtca gtttgtcacg gtgtttttgc cagaagctgc ggccgtgctt gtgatgatcg
     2761 ccaatacaac tgtaatgcgt gtgcgtaacg ttgctgtgtc gatgccggta acggccgcac
     2821 tgggccgagg ccggaactgc catcagcagg agcaaagtcg tcactaccag agtcgcaagt
     2881 agtttcctca tagtttcttc cctctcagta agtccttcta tgcgggagag ctcttagggg
     2941 ggcggccttc attctattgc tgctctcggc gcctgtccag cgatgctggt cctggatcgc
     3001 gatgacgctg gctaagtgct gccaggagat gagctttcgt tttttgggag gcgaaagcgc
     3061 gacagttgcg ccgcgcctga agagaccgta ttatccttcc tcgcaagccg gagtggcgaa
     3121 attggtatac gcaccagact caaaatctgg cgaggtcaca ctcatgtcgg ttcgagtccg
     3181 acctccggca ccattcagat tcttcctcta aatgaatcat tcgccacaag cctggctgcc
     3241 agacctggtc tataccggta aagaattcaa gaacaattta actcttgcta ccgactcgac
     3301 aggacgcgtc ttacgactcg cgtcagcgcg cgaagacgaa gctgttgtgc ggctgaagca
     3361 ttgcgcggtc ttaccgggat tggttaacgc tcactctcat gcttttcaac gcgtgattcg
     3421 cggacgtact gaacaccgga cgactggcac gaccgacagc ttctggacct ggcgcgaaat
     3481 gatgtacagc gccgcagcaa ggttgacgcc tgaagacctc tacgatgctt cgcgcatggc
     3541 gtttatggaa atggcgctca gcggcattac caccgtcggc gagttccact atctccacaa
     3601 tcaggcggat ggttcttcgt atgacgatcc taacctactg gcaaaggagg tggtgcgtgc
     3661 tgccagagac gtcggtctgc gcatagcgct tttgcgcgtt gcctatgcgc gcgcgggcta
     3721 ccaggttgat ccaaacccac gccaatcacg tttcattgag tccacatctg acgtctatct
     3781 gaagaacctt gagcagctgc ggagtgagct cgcaagcatg gcaggggaag cgtgggttgg
     3841 cgtggcgccg catagcgtca gagcggtgcc cctggatcaa ctcaagacga tcgtagcgca
     3901 cgctaatcaa gcacagttac cagttcacat gcatgtagcc gaacagccgg cggaagtttc
     3961 agcttgcatc gcggaacatg gacgctcgcc agttgctttg ttggaaagtg aagggctatt
     4021 aagcgagcgg ttcacggggg ttcacgtgat ccatatcacg gcgaaagcgg cacgaatgat
     4081 cgccgccgcg cgcgcgatgg tttgcgcttg tccctcaacg gaacgcaacc tcggagatgg
     4141 cgttgtgcca atggatctcc tgctgacagc gaacgtcgac gtgtcgttgg gcacagacag
     4201 tcaggcacaa attgatttgt tggaagacgc tcgcgaactt gaatatcact tgcgcttgca
     4261 aaagctggaa cgcgccgtac tctcgctgcc cagcgacgaa gatcaagcgg cgctggcggt
     4321 gcgtctcttt gactgtgcca cgattaacgg agctcgaagc ctgggtgtag ccggtcaacc
     4381 cttagcgaac ggccgcgtcg cggatttttt cactgtcgat ctcgatgacc catcaattgc
     4441 cggcgctacc cgcgacacgc ttctgacgac gatagtcttt tcacttagca agtcggcggt
     4501 gaaggatgtt gtcgtgggcg gcaaacggat cgtcgaagct ggccgacact tcgcgcagga
     4561 agagattacc gagagattca gagagctgca gaaacgactt tggagttagc gcaaacggat
     4621 cacgtaaccg ccaaggcggc aagacacgcc aaggcgcaga gctcataagc ttcagctttc
     4681 aacttgcatg acaactgtcg aaacacttgc ggagctggtc gcgattgact ccgtgtcagc
     4741 gcgccccaat gcggagattg ccgactatct cagcagccgc tgcgagtcaa tgggactcac
     4801 cgtaaagcgt ttcccctacc gcgacgacaa taaccttgag aagt
//
LOCUS       DASUEX010000655         2347 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_333425, whole genome shotgun sequence.
ACCESSION   DASUEX010000655 DASUEX010000000
VERSION     DASUEX010000655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2347
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333425"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(189..1820)
                     /gene="groL"
                     /locus_tag="VFV61_10895"
     CDS             complement(189..1820)
                     /gene="groL"
                     /locus_tag="VFV61_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004211302.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEX4901030.1"
                     /translation="MAAKDMKFHEDARSAMLQGVNTLANAVRVTLGPKGRNVVLAKKF
                     GSPVITKDGVTVAKEIDLPDRYENLGAQMLREVAIKTNDSAGDGTTTATVLAQAIFRE
                     GIKNVTAGANPMSLQRGIQIATDKVVAELERTSQQVKTKEDLTNVATVSANNDREIGQ
                     LISEAMEQVGKDGVVTVEESKTLHTELDIVEGMQFDRGYLSPYFVTNPDRMEANLEEP
                     LILLHEKKISAMRDLLPVLEQSARTGRALLIIAEDIDGDALATLVVNKLRGTIKVCAV
                     KAPAFGDRRKAILEDLAILTGAKVITEDLGIKLENVQAEDLGSAKRVVIDKESTTIVE
                     GAGDAKTIQARVATIKRQIEETTSDYDREKLQERLAKLAGGVAVVRVGAPTETAMKEI
                     KMRVEDALNATRAAAQEGIVAGGGVALLRAAKALDSLSEDDPDVQTGIQIVRRALEEP
                     LRRIAQNAGVDGAVVVGKVTSLKGAKGFNAGSGEFEDLIAAGIIDPTKVVRTALQNAA
                     SIAGLLMTTDAAVTDAPEPKKAAPSMPGGGDDYDY"
     gene            complement(1863..>2347)
                     /locus_tag="VFV61_10900"
     CDS             complement(1863..>2347)
                     /locus_tag="VFV61_10900"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEX4901031.1"
                     /translation="TRAAEQLALDVQARFEQFKSRLQQETDETRQRLNRAADERTERS
                     KAEFIANLLPTLDNLERAIEAAEAGGSMEQLVAGVEQTAESFKSALSAAGVHPLVTEG
                     ELFDPELHEAVETIPAEADLDGKVMNEYTKGYRIGERLLRPARVAVGRAPAKAQSAND
                     "
BASE COUNT          436 a          707 c          605 g          599 t
ORIGIN      
        1 agcgagactg tcatcgatcg ttcaaaataa cgacatccaa cccgagggtt agatgcaact
       61 gagcatatgc gccgttgtgt ttcaatgcaa tacctatctc ccggctcgac cgcttgcaaa
      121 tcttcggcca gcggcccaga cgtaaccggc tgaattcccg caaagtccgc ggcgcagccc
      181 gcagctaatt agtaatcgta gtcgtcgcca ccgccgggca tggatggcgc ggcttttttg
      241 ggttccggtg catcggtgac cgccgcgtcg gtcgtcatca agagtccggc gatcgaagca
      301 gcgttttgca aagcagtacg cacgaccttt gtcggatcga taatgcccgc cgcgattaag
      361 tcttcaaact cgccggatcc cgcattgaat cctttggccc cttttagaga tgtcactttg
      421 cccacgacga ccgcaccgtc caccccggca ttctgggcga tacgccgcag cggctcttca
      481 agcgcgcgcc ggacaatctg tattcctgtt tggacgtcgg gatcatcttc ggaaagtgaa
      541 tcgagagctt ttgccgctcg caagagtgcc actccgccac ccgccactat gccctcctgt
      601 gcggctgcgc gcgtggcgtt gagcgcatcc tcgacgcgca ttttgatttc cttcatcgcc
      661 gtttccgtcg gcgctcccac tcttacaaca gcaacaccgc cggctaattt cgccaggcgt
      721 tcctgcaact tctcgcggtc gtagtcagac gtagtctcct cgatttggcg tttgatagtg
      781 gccacgcgtg cctgaatggt tttggcgtcc cctgcacctt caacgatcgt ggttgattct
      841 ttatcgatca ccactcgttt cgccgaaccc aaatcctcgg cttgtacatt ttcaagtttg
      901 attcccagat cttcagtgat gacctttgcc cccgtcagga tcgccaggtc ttcgaggatt
      961 gccttgcgcc gatcaccaaa cgccggagct ttgacagcgc aaactttgat cgtgccgcgc
     1021 agtttattta cgacgagtgt ggcgagagcg tcaccgtcga tatcttccgc aatgataagc
     1081 agcgcgcggc ctgtacgtgc cgactgttca agcaccggca gcaagtcacg catcgcagaa
     1141 atcttcttct catgcaataa aattagtggt tcttcgagat ttgcttccat ccgatcaggg
     1201 ttggtaacga aatatggtga caggtaaccc cggtcgaact gcattccttc tacgatatcc
     1261 aactcagtgt gcagcgtctt tgattcctcg acggtaacga cgccatcctt gccgacctgc
     1321 tccatcgctt ccgaaatgag ctggccaatc tcgcggtcat tattggcgga caccgtggca
     1381 acgtttgtta gatcctcttt ggtttttacc tgctgtgagg tgcgttcaag ttcagcgacc
     1441 accttatccg tcgcgatttg aatgcctcgt tgcagcgaca tcggattagc gcctgcggtc
     1501 acgttcttga tgccttcacg gaagatggcc tgggcaagta cggttgctgt ggtggtgccg
     1561 tcgccggcgg agtcgttggt cttgatggcg acctcacgca gcatttgcgc gccgaggttt
     1621 tcatagcggt ctggcaaatc aatttctttc gccacggtga caccatcttt ggtgattacc
     1681 ggagatccaa atttctttgc caacaccacg ttgcgtcctt ttgggcccag ggtgacacgc
     1741 acggcattcg caagcgtgtt aacaccctga agcatcgcgc tccgtgcgtc ttcgtgaaac
     1801 ttcatgtctt tggcagccac agtcttctcc ttgagtcact aataaccggt tggtttgttg
     1861 gcttaatcgt tcgcgctttg tgccttcgcc ggcgcgcgtc caactgctac tcgagccggg
     1921 cgcaacagcc gctcgccgat gcgatagcct tttgtatact cattcatcac ttttccgtcc
     1981 aaatctgctt ccgcgggaat tgtctcgaca gcttcatgta actccggatc gaacaactca
     2041 ccctcagtca ccagagggtg gacgcccgca gcgcttagcg ctgacttaaa actctcggca
     2101 gtttgttcca cgcccgcgac aagttgttcc atggaaccgc cggcttcagc ggcctcaatt
     2161 gcccgctcca gattgtcgag cgtgggcagc aggttcgcaa tgaattccgc tttgctgcgc
     2221 tcagtccgtt catcggcggc ccgattgaga cgctggcggg tctcgtccgt ctcttgttgc
     2281 agtctgctct tgaattgttc gaagcgggct tgaacgtcga gcgctaactg ctccgccgcg
     2341 cgcgtgc
//
LOCUS       DASUEX010000656         3986 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_669372, whole genome shotgun sequence.
ACCESSION   DASUEX010000656 DASUEX010000000
VERSION     DASUEX010000656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3986
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_669372"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            93..3287
                     /locus_tag="VFV61_10905"
     CDS             93..3287
                     /locus_tag="VFV61_10905"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4901032.1"
                     /translation="MLRRSLAIVCLCLSTFASITALAQELTQPSPSPSPQSSPSASPD
                     TRPPFEAAFDRLEWRSIGPANMGGRTADVEGVPGDPNTVFVASASGGLWKTVNGGISW
                     KPIFERQGTISLGDIALAPSNPDVVWAGTGESNTRNSVSFGDGVYKSTDGGKTWQHMG
                     LRDTERISAIVIHPQNPDIVYVGALGHAFGANEDRGVFMTTDGGRTWTKTLYIDKEHG
                     VSDLDMDPTNPNILYAGMWSFERKAWTHRSGSEKGGVFKSIDGGRTWNKLSNGLPKLI
                     GRIGVRVAPSNANVVYAIVEAKEGTLYRSDDRGETFRQVSKETRIVSRGFYYTTVRVD
                     PTNENKVYAVASTLFVSIDGGKTFRSITGRTHIDYHALWIDPKNPKRMWQGQDGGIAV
                     SYDAGESWEYVNNIPLGQFYQIHADNRLPFYNVMGGLQDNGAWTGPSRSREPAGIMND
                     DWRMISFGDGFYVLNSPDDPDVYISESQGGNILRTDFRTREQQEVNPWGRGSGDGPAL
                     GEKFRFNWNAPIVQSPHNGNTIYLAGNVVFKSTDFGRTWEQISPDLTTNDPEKQKDAG
                     GPIAFENSTAEYHTTITAIAESPVQKGLIWVGTDDGNLQVTTDAGKNWANVIKNLPGL
                     AANSGVSHVEPSRTNAQLVYAAFDRHMLDDFRPYVFKTMDGGKSWSNITGNLPAKAYV
                     QVVREDPKNPNLLYVGTELGLFASYSGGNDWIRLGLKNLPFVAVHDIKIHPRENDLIL
                     ATHGRSVWILDDAAPIQQMNSELLSREAHLFPVRPALRFTSRFTRYGIGDKVFAGPNP
                     SYGALITYYLKEKPDDKTTFKVEIFDQTGKLVQALNRPAREKGLNRVAWNLRFGGAEV
                     RQPPPEEETATGGPPRGPQALPGSYIVRLTLGEKKIEERVEVRLDPTVNTPIADLQAQ
                     LDLTIKLRDMQSSANTALRFLDSIKDQLKHTQTTMKNLNKEPDKEMTKALEEYVKKID
                     ELQDKLARRSEGLGLGGKSRVSDRVGDLFFSIDATNAAPTPYQRKYFEEVEPEFRERM
                     AEVNKFIAETVPQWNEKLRGWNAPTLTTRKPVEF"
     gene            complement(3359..>3986)
                     /locus_tag="VFV61_10910"
     CDS             complement(3359..>3986)
                     /locus_tag="VFV61_10910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4901033.1"
                     /translation="HTAEIYIERTAKSPEAMQRRKIVIEATANSLKIRGEKGDVGFIS
                     RIFGSSPHEKVTLKLPRQISLVTQGVNGAVTVGEIDGPVEVHGLNGKVEIAQATGSAE
                     FHGINGNISVALTRLEKEAVSLNGVNGNIELRLAEGVNVELEAHGMNGRVSSDLPDFV
                     LDEQRHGNYHARVGSGGNSISANGINGNIRLSRVASAVVKTESAKVKV"
BASE COUNT          979 a         1102 c         1069 g          836 t
ORIGIN      
        1 actggcagcg caaacttgcg gcgcaagact taacccgaat ttgacagctc aaaagtacaa
       61 accgaaacgc tacagatcat tcaggagaac cgatgcttcg acgcagcctc gcaattgtct
      121 gtctctgcct gtccacattt gcttcaatca cagccttagc tcaagagctc acgcagccct
      181 caccgtcgcc gagtccacag tcatcaccga gcgcctctcc ggacacgcgg cctccgtttg
      241 aggctgcatt tgatcgtctt gaatggcgca gcattggtcc cgcgaacatg gggggacgca
      301 cagcagacgt cgaaggcgtg cctggcgatc caaacacagt cttcgtggcc agtgcgtccg
      361 gaggtttgtg gaaaacagtg aatggcggca tcagctggaa accaatcttc gaacgacagg
      421 gaacgatttc gctgggagac attgcgctgg cgcccagtaa ccctgacgtt gtctgggccg
      481 gcaccggcga atcaaacacg cgtaacagcg tctcttttgg cgatggcgtc tacaagtcta
      541 ctgacggcgg aaagacctgg cagcacatgg gactccggga caccgaacgc atttcggcaa
      601 tcgtcattca tccgcagaat ccagacatcg tttacgtggg cgcactaggt cacgcgtttg
      661 gcgctaacga agaccgcggt gtgtttatga caacagacgg cggccgaacc tggaccaaaa
      721 ctctttacat cgacaaggaa cacggagttt ccgatctgga tatggatccc accaatccca
      781 acattctcta cgccggcatg tggagcttcg aacgaaaagc atggacacat cgcagcggca
      841 gcgaaaaagg cggcgttttc aaatcgattg atggcggccg aacctggaac aagttgagca
      901 atggcctgcc gaaattgatt ggacgcattg gcgtgcgagt cgcgccgagc aacgccaacg
      961 tcgtctacgc catagttgaa gctaaagagg gaacgctcta tcgctcggat gatcgcggtg
     1021 agacttttag acaggtttcc aaggaaacgc ggattgtctc ccggggtttc tactacacaa
     1081 cggtgcgcgt tgaccctacg aatgagaaca aagtttacgc agtggcatca acccttttcg
     1141 tttccatcga cggtggcaag acttttcgct ccatcaccgg caggactcat atcgactatc
     1201 acgccctctg gatcgatccc aaaaacccca aacggatgtg gcagggccag gatggcggga
     1261 ttgctgtgtc ctatgacgcg ggcgaatcgt gggagtatgt gaacaacatt cctctgggcc
     1321 agttctatca gattcacgcc gacaaccgct tgccgtttta caacgtcatg ggcgggctcc
     1381 aggacaacgg agcgtggacc ggtcccagtc gctcacggga gccggccgga atcatgaacg
     1441 acgactggcg gatgattagt tttggggatg gcttttacgt cctcaatagt cctgacgatc
     1501 cggacgttta catttccgaa tctcagggtg gaaatattct gcgcaccgat tttcgtactc
     1561 gcgaacagca ggaagttaat ccctggggac gtggcagtgg cgacggaccg gcgctcggtg
     1621 agaagttccg ctttaactgg aatgcgccga ttgtgcaatc gcctcacaac gggaacacga
     1681 tctacctcgc cggcaatgtt gtcttcaaat caactgactt tggcagaacg tgggaacaaa
     1741 tcagtcccga tctgacaacg aacgatcccg aaaaacagaa agacgccggc ggaccaatcg
     1801 cttttgagaa ttccaccgcc gaataccata caactatcac cgcgattgcg gagtcgccgg
     1861 ttcagaaagg ccttatctgg gttggcaccg acgacggcaa tctccaggtc acaacggatg
     1921 ccggtaagaa ctgggctaat gtcataaaga accttcccgg cttagcggcc aattccgggg
     1981 tttcacacgt cgagccgtcg cgcacgaatg cccaactcgt ctacgctgca ttcgatcggc
     2041 acatgctcga tgattttcgt ccctatgtgt tcaaaaccat ggacggcggc aaatcatgga
     2101 gcaacattac cggcaactta ccggcgaagg cttacgtgca ggttgtgcgc gaggatccga
     2161 aaaaccccaa cttgctttac gtcggcaccg aactaggttt gttcgcgtcc tacagtggcg
     2221 gaaacgattg gatcagactt ggcctgaaga acctcccctt tgttgccgtg cacgacatta
     2281 agattcaccc gcgcgagaat gatctgattc ttgccactca cggtcgcagt gtttggatcc
     2341 ttgacgatgc cgctcccatc caacaaatga attcggagtt gttgagtcgc gaggcacatt
     2401 tgtttcccgt ccgtcctgca ttgcgcttca catcgcgatt cacgcgctat ggcatcggcg
     2461 ataaagtttt cgccggacca aatccttcct atggagcgct gattacttat tatttgaaag
     2521 agaagcctga cgataaaacg accttcaagg tagagatttt tgatcagacg ggaaaactcg
     2581 tgcaggcatt gaaccgaccg gcaagagaaa aaggtctgaa tcgcgtggcc tggaacctgc
     2641 gcttcggcgg cgccgaagtg cgacagccgc cacctgaaga agagacggcc acgggcggtc
     2701 ccccacgcgg gccgcaggca ttgcccggca gctacatcgt caggctgacc ttaggtgaga
     2761 aaaaaatcga ggaacgggtt gaggttcgtc ttgatcccac cgtgaatacg cccatcgcag
     2821 atctgcaagc gcagctcgat ctgactatca aactgaggga tatgcaatcc tcggccaata
     2881 cggcgctgcg gtttctggac agcatcaagg accaactgaa gcacacgcag acgacgatga
     2941 agaatctgaa caaagaaccg gacaaggaga tgaccaaggc gctcgaagaa tacgtgaaga
     3001 agattgatga gcttcaggac aagctcgcgc ggcgttccga gggcctcggc ctcggcggca
     3061 agtcgcgagt atcagatcga gtgggtgatc tgttcttttc gatcgacgcg accaatgccg
     3121 ccccgacacc ctaccagcga aagtatttcg aagaagtgga gccggagttt cgcgagcgca
     3181 tggcggaagt gaacaagttc attgccgaaa cggtgccgca gtggaacgag aagttgcgtg
     3241 gctggaacgc acccacacta accacgcgaa agccagtcga attctaaagc aagttaacta
     3301 ctaacaagaa aagggcacgc tgatcacgtg ccccttttct ctttttgcag ccaggaggtt
     3361 agaccttaac ttttgctgat tctgtcttca caaccgccga agcgacacga ctcaaacgaa
     3421 tgttgccgtt gataccatta gccgagatcg agtttccgcc actgcccacg cgagcatggt
     3481 agttaccgtg gcgttgctca tccaacacga agtcaggaag atcagacgag acgcgaccgt
     3541 tcatgccgtg cgcttcgagc tcaacgttaa caccctctgc gaggcgcaac tcgatgttgc
     3601 cgttcacgcc attaagactg actgcctctt tctccaaacg ggtaagcgcc acggaaatat
     3661 tgccgttgat gccgtgaaac tcagcggagc cggtcgcctg cgcgatttcc actttcccat
     3721 taagtccgtg tacctcaacg ggaccatcga tttcaccaac ggtgactgcg ccgttaacgc
     3781 cctgcgtcac aagtgaaatc tgacggggaa gtttcaaagt aactttctcg tgtgggctcg
     3841 aaccgaagat acgtgaaata aagccaacgt cgcccttttc gccgcgaatt ttcagactgt
     3901 tcgccgtggc ttcgataaca atcttacgtc gctgcatggc ttcgggactc ttggcggtac
     3961 gctcaatata gatctcggcg gtgtga
//
LOCUS       DASUEX010000657         2637 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_670069, whole genome shotgun sequence.
ACCESSION   DASUEX010000657 DASUEX010000000
VERSION     DASUEX010000657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2637
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_670069"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..903)
                     /gene="obgE"
                     /locus_tag="VFV61_10915"
     CDS             complement(<1..903)
                     /gene="obgE"
                     /locus_tag="VFV61_10915"
                     /inference="COORDINATES: protein motif:HMM:NF008955.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEX4901034.1"
                     /translation="MFIDRVKIHVQGGHGGNGVTAFRREKFVPRGGPSGGEGGRGGDV
                     WIIADSSLNTLLHLRFNPEHIAGRGMHGEGSNRSGRDGDNVLVRVPVGTQIFEATSGE
                     LLQDLTVDGTRWLAARGGRGGFGNSHFATSTNRAPRYHQDGSAGEELELQLELKLLAD
                     VGLVGFPNAGKSTLISTISAAKPKIADYPFTTLEPNLGVVDLGDFRTFVVADIPGLIE
                     GAHEGAGLGDRFLRHIERTTLLLHLVDVSSFSGRETVDDYRTVNHELAAYNPTLAARP
                     QIVVATKIDALEDPERLESLRQAAT"
     gene            complement(979..1251)
                     /gene="rpmA"
                     /locus_tag="VFV61_10920"
     CDS             complement(979..1251)
                     /gene="rpmA"
                     /locus_tag="VFV61_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010545913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEX4901035.1"
                     /translation="MAHKKGVGSSRNGRDSQSQRLGLKKFGGERVLGGNILARQRGTK
                     WKPGKNVGRGKDDTLFALIEGVVKFEDKGRGGKFISVYPLEQASPA"
     gene            complement(1331..1627)
                     /gene="rplU"
                     /locus_tag="VFV61_10925"
     CDS             complement(1331..1627)
                     /gene="rplU"
                     /locus_tag="VFV61_10925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEX4901036.1"
                     /translation="MPFAIIKTGGKQFVVEKGSTVRIPSLAAEAGKTVEIDALLTGDQ
                     GGAPVVGAAKISATVVEHGRGTKFLVFKKKRRKHYKRTQGHRQGYTAIRIDSIG"
     gene            complement(1704..>2637)
                     /locus_tag="VFV61_10930"
     CDS             complement(1704..>2637)
                     /locus_tag="VFV61_10930"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEX4901037.1"
                     /translation="EIGFPVIVKASAGGGGRGMRIVRSADDLGRALEAASTEAAAAFK
                     DGSVYIERYLENPRHIEIQVLADEYGDCIHLGERECSIQRRHQKLLEEAPSPILSAQL
                     RNEMGNAAVAACKKLGYSSAGTVEFLLDQDNRFYFMEMNTRIQVEHAVTEMVTLADIV
                     RNQIRIAAGERLGYTQDDLLIVGHAIECRINAENPETFAPSPGTITAFNLPGGPGVRV
                     DTYVYSGYKVPPFYDSMIAKVIVHARTRELAIARMKRALEAMVVEGIKTTIPLHLKIM
                     DDPAFQAGDISTSFMEHFLKQNGAKKNGALTTAR"
BASE COUNT          590 a          790 c          657 g          600 t
ORIGIN      
        1 ggtagccgcc tgcctcagag actcgagtcg ctccgggtct tccagagcat ctatcttcgt
       61 ggcaacgaca atttgcgggc gcgcagccag ggtcgggtta tacgccgcca actcgtgatt
      121 cacggtgcga tagtcgtcta ccgtttctct tcctgaaaag gacgagacat caaccagatg
      181 aagtaacaat gtcgtccgct caatgtggcg cagaaagcgg tcgccgaggc ccgcgccttc
      241 gtgggcgcct tcgatgagac caggaatatc cgccaccacg aacgtgcgaa agtcgcctaa
      301 atcaacaacc cccaaattgg gttcgagcgt tgtaaaaggg tagtctgcga tttttggttt
      361 cgccgccgag atagtcgaaa tcaaagtcga cttgccggca ttaggaaacc cgaccagccc
      421 cacatccgcg agcaacttca attcgagctg caactccaac tcttcgccgg cactgccatc
      481 ctggtgataa cgaggcgcgc gattagtcga agtcgcaaag tgagaattac caaatcctcc
      541 gcggccgcca cgggcagcga gccacctcgt accgtcgact gttaagtcct gcaataactc
      601 gccgctggtt gcttcaaaga tctgagtgcc aaccgggacg cggaccagaa cattatcgcc
      661 atcgcgtccc gatcgattag agccctcgcc gtgcatgccc cggccggcaa tatgttcagg
      721 attgaaacga aggtgaagca gagtgttcag ggaagagtca gcaataatcc agacatcacc
      781 tccgcgtccg ccttcgccgc ctgaagggcc acctcgcgga acaaacttct cgcgccgaaa
      841 agctgttacg ccattgccac catgccctcc ctggacgtga atcttaacgc ggtcgataaa
      901 catttaaatc gaatgcgacg ccaggctcag ttaattacct cgcctgggcg tcgcatgaga
      961 atgatctcgg tgcgtcacct aagcgggaga cgcctgctcg aggggataga cgctgataaa
     1021 cttgccaccg cgacctttgt cttcaaactt cacaacacct tcaatcagag cgaagagggt
     1081 gtcgtcttta ccgcgaccca cattctttcc tggtttccac ttggtgccgc gctgccgcgc
     1141 caggatattg cctcccagca cgcgctcgcc accgaatttt ttcaaaccca atctctgcga
     1201 ctgcgagtcg cgaccatttc gagatgagcc aactcccttt ttatgtgcca taacttattt
     1261 ccgattgttg attgccgatt tccgattgtt accgcggtaa ctcgttaatt ggcatctctc
     1321 aatgggcctc ctagccaatt gaatcaattc gtatcgccgt atagccctga cggtgtccct
     1381 gagtgcgctt gtaatgcttg cggcgcttct tcttaaacac caaaaacttc gtgccccgtc
     1441 catgctcgac gacagtcgcg gagatcttgg ccgcgccaac aaccggagcg cctccctgat
     1501 caccggtcaa taaagcgtcg atttcgacgg tctttcccgc ttccgctgcg agggagggaa
     1561 tccgaacggt gcttcccttt tctactacaa actgtttccc gccggttttg attattgcaa
     1621 atggcattga ttcacctcac agacgaaact gaccctcgga aaagtcgatt attataggca
     1681 tccgggccgt aaacagcaaa cgctcagcgt gcggttgtta gcgcaccgtt cttctttgcc
     1741 ccattttgtt ttagaaagtg ttccatgaaa ctagtggaga tgtctcctgc ctggaacgcg
     1801 ggatcatcca tgatcttcaa gtgcaatgga atcgtcgtct tgatcccctc taccaccatg
     1861 gcctccaaag ctcgcttcat gcgtgctatc gccagctcgc gggtccgcgc atgaacgatc
     1921 accttggcaa tcattgaatc gtaaaacggg ggtaccttgt aaccagagta gacgtaggtg
     1981 tccaccctca ccccgggacc gccgggcaag ttgaaagcgg tgatagttcc tggcgaaggc
     2041 gcgaaggttt cggggttctc ggcattaatc cggcactcga tagcatgacc aacgatcaac
     2101 agatcatctt gtgtgtaacc gagtcgctcg ccggcggcga tgcggatctg attacgcaca
     2161 atatctgcca gcgtcaccat ttcagtgacg gcgtgttcca cctgaatgcg cgtattcatc
     2221 tccatgaagt agaaacggtt gtcctgatcg agaagaaact cgacggttcc ggcgcttgaa
     2281 tatccaagct tcttgcaggc cgccacagcc gcattcccca tctcattcct cagttgcgct
     2341 gacaagatcg gcgaaggcgc ctcctccaaa agtttctgat gccgtcgttg gatcgaacac
     2401 tcgcgctctc cgaggtgaat acagtcgccg tattcgtcgg ccagcacttg aatttcaatg
     2461 tgacgcggat tctcgagata acgttcgatg taaacgctgc cgtctttaaa cgccgccgcc
     2521 gcctccgttg acgcggcctc cagagcgcga cccagatcat cggcagagcg tacgattctc
     2581 atgccgcgac cgcccccacc tgcggaagct ttaacgatca cgggaaagcc tatctcg
//
LOCUS       DASUEX010000658         5307 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_546361, whole genome shotgun sequence.
ACCESSION   DASUEX010000658 DASUEX010000000
VERSION     DASUEX010000658.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5307
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_546361"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="VFV61_10935"
     CDS             <1..226
                     /locus_tag="VFV61_10935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4901038.1"
                     /translation="TICYELLEEARRLGKHMNGGRDNKQTTLRVNPEVAKALRGSEKD
                     VLNETEEYLGPVDINSDQMIHQEQFDFAFI"
     gene            409..1392
                     /locus_tag="VFV61_10940"
     CDS             409..1392
                     /locus_tag="VFV61_10940"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX4901039.1"
                     /translation="MSKVGNLWKTMLAGGAGVAALAAMNASIRRNASEPDDSALGGEA
                     RFFDWKYGRIFYKTAGIGHAGPPLLFIHGVGAGSSSFMWRKNFDALARDFRVYAFDLL
                     GFGLSDKPSGAPYSADLYVELITDFIREVCGYPANVVASSLGAAYAIRVADEHPELIK
                     AMVLNAPTGADKLNTRPGMAGAAFYGLLQSPVLGTSFYNVMASERGIRDYARKHLFYD
                     HHRVTDRLVANLYATSHQSGAQHAIAAFLSGYLNTDTRAAFSRLTQPVILVWGKQDMT
                     TPVENGLALLSTNPAARLEKFDYCRMLPEQEHPEKFNTLVRDAFLARSAAA"
     gene            1657..4737
                     /locus_tag="VFV61_10945"
     CDS             1657..4737
                     /locus_tag="VFV61_10945"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX4901040.1"
                     /translation="MRHALLRSFASQAIGLGICLLLSCVFTLPVLAQTQSTTGTIEGT
                     VLDANGAALPGASVEVKNIATNFTKTFETDEDGRFVALSLPPGRYTVTVTKSGFATTV
                     VESFELTVGRALSLPISMKVSGVEERVTVTATPTVDTTKVESSTTIDERAVSTTPILG
                     RKFEDLLTLTPGVSIVQGPDGDEINFAGQRGIFNNVSLDGGDYNNGFFGEQAGGQRAA
                     IDITLEAVKEFQVVATGASAEFGRTAGGIINVITKSGTNEFHGSLFHYQRLEALTANA
                     SNGEPLTDFRREQFGGTIGGPIKRDKAFFFFASENIRERLTRANLSRQIGATACPVSA
                     PTILVNETLINSNFDCQRLALISFFRASRSQEEGLPVNRPIRNNALLGKIDVDLTSNH
                     RLTLSHNFDYSKNENQTFDVPTYGNSANGTEGPSKINAFNANLLSTLSPTRVNEFHFT
                     YSREERPRAAVASNVPADTAMGGFFSSAEPTFRFGHPFFLGPNIDETFWRTQIKDNFS
                     IVSGRHQVKFGGEWMHSVNSQVFRGFFQGRYIFDSVAGFLHYATPGAVGSGFGPNAGR
                     CTNGSWTDLTLGAPPCTGATTPLLLFLQGAGPNGPATDAAGASDIKNEDYGLFVQDKW
                     QVRPNFTLNLGLRWEAQIFPEPEVAPSATAYGIFLSDPRFPSDGTLHSPKKQFQPRVG
                     FAWDVTGNGKSVLRGSYGIYYGRQNMLTQVGSITTNGVQQQTIAGGLFANPSVRPTWP
                     GLVTPTASSCSTHAGFTVAGPFNNPFPCFSGVRLFSKDYANPRIYTTNVGFEQELASN
                     LSIYFDFTHAKGVHLTRFLDYARLLGSDGAPVFGDYLGEIMVASAVGKSQYRAFTVGM
                     RKRFSRRFGFEANYTFAKDEDDDSNERDPFVDRAADFFNLQKDYSLADRDIKHRFNMF
                     ANVDIPAGFELSTRIQARTAQPKSLAGCATRCFPRNTDRKNNEYFSFDWRLQRPFRFG
                     EKYALIPMIEMFNTFNNANNIDPTISSALFNFDGFLRLGVGDPRQLQLALKFTF"
     gene            complement(4876..>5307)
                     /locus_tag="VFV61_10950"
     CDS             complement(4876..>5307)
                     /locus_tag="VFV61_10950"
                     /inference="COORDINATES: protein motif:HMM:NF012900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEX4901041.1"
                     /translation="RLRVNTIQICDRLELQSYDALRAALPGISLVQVVHVNDVDSVAE
                     AISVAAFVDAVLLDSGNQSLPVKELGGTGRTHDWNLSVQIREAIDVPLFLAGGLKAEN
                     VAEAIRQVRPFGVDVCSGVRTEGHLDEEKLKRFFGAVRGAG"
BASE COUNT         1176 a         1551 c         1347 g         1233 t
ORIGIN      
        1 gacgatttgt tatgaactgc tcgaggaagc gcgccgttta ggaaagcaca tgaacggcgg
       61 ccgcgataat aagcaaacga cgttgcgcgt gaatccggaa gtcgcaaaag cattgcgcgg
      121 atcagagaag gacgtcttaa atgagaccga agagtatctc ggaccagtcg acatcaatag
      181 cgatcagatg attcaccagg agcagtttga ttttgctttt atttgagtcg ggcttaattc
      241 ctgggtgttg aactgttttt gaactttgta cttagagctt tgtactttgt tctagtctcg
      301 ttgcttgaat ttttctttga acacttcgag tctcctaagc aagcaacgtt ctaagtacaa
      361 agttcaaagc tcaaaaagca agcctatcgg caaagtctag tttccatcat gagcaaggta
      421 ggaaatctgt ggaagaccat gttggcgggc ggcgccggag tggcggcgct ggcggcgatg
      481 aacgcctcca tacgccgaaa cgccagtgaa cccgacgata gtgcgctggg tggcgaagca
      541 cgcttcttcg attggaagta cggacggatt ttctacaaga ccgccggcat tggccacgcc
      601 ggaccgccat tgcttttcat tcacggcgtc ggggccggtt cctccagttt catgtggcgc
      661 aagaacttcg acgctctcgc gcgcgatttt cgcgtctatg cttttgatct gctgggattt
      721 ggcctttctg acaaaccgag cggcgctcct tactcggccg atctgtacgt tgagctaatc
      781 actgatttca ttcgtgaggt ctgcggctac ccagctaacg tcgtggcgag ttccctcggc
      841 gcggcttacg ctattcgagt tgcagacgaa catcccgaac tgattaaggc aatggtactc
      901 aacgctccca ctggtgctga caaactaaac actcgaccgg gcatggcggg cgcggctttc
      961 tatggcttgc tgcaatcgcc ggtgctcggc acttcgttct acaacgtcat ggccagcgag
     1021 cgtggcattc gcgactatgc gcgcaaacat ctcttttacg atcatcatcg cgttaccgat
     1081 cgactcgtgg ccaacctcta cgccaccagc caccaatccg gtgcgcaaca tgcgatcgca
     1141 gcttttctat cgggatacct caacactgac acgcgtgctg ccttttcccg gctgacacaa
     1201 ccggtgattc tcgtttgggg caaacaggac atgactacgc ctgtggaaaa cgggctggcg
     1261 ttgctgagca caaatccggc agcgcggttg gaaaaatttg attactgccg catgttgccc
     1321 gaacaggaac acccggaaaa atttaacacc ctcgtccgcg acgctttcct cgcccgatca
     1381 gccgcggcgt aacagcaatc attaatcgag aatcgaatcg gggctggacg tggcctttgg
     1441 gtagttgaac ctacttcaag ctttcgcagg acgcctcacg atttccgatt cacaattcac
     1501 gaattcacaa ttcccgagtc ctgattttac taacttattg acacctacaa ctatctttca
     1561 ataagatgac ccccttccca agcggtcaac gggcggaatg cacttctggt tctgaactcg
     1621 gtaattcgaa ctcaaaaaat cgacaggaga acccccatgc gtcacgcatt gcttcgctcc
     1681 tttgcctccc aggcaatcgg actcggtatt tgtctgcttc ttagttgtgt atttacctta
     1741 ccagtcttag cccagaccca gagtacgacc ggaacaatag aaggcacagt gttggacgcc
     1801 aatggcgctg ctttgcccgg cgccagcgtt gaggtgaaga acatcgccac caacttcacg
     1861 aagaccttcg agaccgacga ggacggacgc tttgtggcgt tgagcctgcc tcccggccgg
     1921 tacacggtaa ccgtaaccaa gtcaggtttt gcgacgaccg tggttgagag ttttgagttg
     1981 actgtcggcc gggccctgtc gctgccaatt tccatgaaag tttccggagt tgaagagcga
     2041 gtgacggtca ccgccactcc caccgtcgac accaccaagg tcgaatcaag caccacaatc
     2101 gacgaaagag ccgtcagcac cacgccaatt ttgggacgca agtttgaaga tctgctgact
     2161 ctgactccgg gcgtcagcat cgtgcagggc cccgacggcg atgaaataaa ctttgccggc
     2221 cagcgcggaa tcttcaacaa cgtcagcctt gacggcggcg attacaataa tgggttcttt
     2281 ggtgagcaag ctgggggtca acgcgctgcc atcgatatca ccctggaagc ggtgaaggag
     2341 tttcaggtgg ttgcaactgg cgccagcgcg gagtttggcc gtaccgcagg tggcatcatc
     2401 aatgtcatca ccaaatcagg gaccaatgaa tttcacggta gtctcttcca ctatcaaaga
     2461 ctcgaagcgc tgacggctaa tgcctccaat ggcgagccgc ttaccgactt ccgccgcgag
     2521 caattcggcg gcacgattgg cggtccgatc aaacgtgaca aggccttctt cttcttcgcc
     2581 agcgaaaaca tccgtgaacg tctgacgcgc gcaaacctga gccgccagat aggagcaact
     2641 gcctgccctg tctccgcgcc gaccattctc gtgaatgaaa ccctgattaa ttctaatttc
     2701 gattgccaac gactcgcctt gatttcattt ttccgggcca gccgcagcca ggaagaaggt
     2761 ctgccagtca atcgtcccat ccgcaacaat gccttactgg gcaagatcga cgttgacctg
     2821 acctccaatc accggctcac gctttctcat aacttcgatt attcgaagaa tgagaaccag
     2881 accttcgatg ttccgactta cggcaattca gccaacggca cagaaggacc ttcgaagatc
     2941 aacgccttca acgcgaatct cttgtcgacg ctctcgccaa cgcgagtaaa cgagtttcac
     3001 ttcacctatt cacgagaaga gcgaccgcgt gccgctgtag cttcaaatgt tcctgcggac
     3061 acggcaatgg gtggcttttt cagctcggct gaaccgactt tccgctttgg tcatccgttc
     3121 tttctcggtc cgaacatcga cgagacattt tggcggactc aaatcaaaga caatttctcg
     3181 atcgtcagcg gccggcacca ggtgaaattc ggcggcgagt ggatgcatag cgtgaactcg
     3241 caagtatttc gtggtttctt tcagggccgc tacatcttcg acagcgtcgc cggttttctg
     3301 cactacgcca cgccgggggc agtcggctcc gggtttggac cgaacgcggg acgttgtacc
     3361 aacggcagct ggaccgactt aacgctcggc gctccgcctt gcaccggcgc gaccacgccg
     3421 ttgttactct tcctgcaagg tgctggtcca aatgggcccg ctactgatgc ggccggggcg
     3481 tctgatatta agaatgaaga ctacggactg ttcgtccagg acaaatggca ggtacgaccc
     3541 aacttcactt tgaatttagg tctccgttgg gaagcgcaaa tcttcccgga accagaagtc
     3601 gcacccagcg cgaccgcata cggaatcttt ctctccgatc cgcgctttcc ttccgatggc
     3661 acgctccaca gcccgaaaaa gcagtttcag ccgcgcgtag gattcgcctg ggacgtcact
     3721 ggcaatggta agtcggtgct gcgcggcagc tacggcatct actacggacg tcagaacatg
     3781 ctgacgcagg ttggctcaat tacaaccaac ggcgtgcaac aacagacgat cgccggtggt
     3841 ttgtttgcca acccctccgt ccgcccgacc tggccgggtc tcgtgacgcc gacggccagc
     3901 tcctgctcga ctcatgcggg cttcacggtt gccggacctt tcaataatcc cttcccgtgc
     3961 tttagcggtg tccggttatt cagcaaggat tatgcaaacc cgcgcatcta caccaccaac
     4021 gttggcttcg agcaggagtt ggcaagtaac ttgtcgatct attttgattt tacccatgcc
     4081 aagggcgtcc acctgactcg ctttcttgac tatgcccgcc tgctgggctc cgatggggca
     4141 ccggtctttg gcgattacct gggcgagatt atggtggcta gcgcggtcgg aaaatctcag
     4201 taccgcgcct ttacggtagg aatgcgcaag cgtttcagtc gccgcttcgg ctttgaggca
     4261 aactacactt ttgcgaaaga cgaggacgac gattccaacg agcgtgatcc tttcgtggat
     4321 cgtgctgccg acttcttcaa ccttcaaaaa gactattcgc tcgccgatcg cgacatcaaa
     4381 catcgcttca acatgttcgc gaacgttgat attccggcgg gcttcgaact gagtacgcgc
     4441 attcaggcgc gcaccgcgca accaaaatct ctagccgggt gcgctactcg ctgtttccca
     4501 cgcaacacgg accggaagaa taacgagtat ttcagcttcg attggcgctt acagcgtcca
     4561 ttccggtttg gtgagaaata cgcgctgatt ccgatgattg agatgttcaa taccttcaac
     4621 aacgcgaaca acatcgatcc cacgatcagc tcggctctat tcaactttga cgggttcctc
     4681 cgtctgggtg taggcgatcc gagacaattg caactggcgt tgaagttcac gttctaggcc
     4741 gaaggccaag ttaaagaggg gaacagggga acgggggatg gcggttgaag gctttccctg
     4801 ttcccttttt ccattctccc cgttcccctt ttcccttgtt cctgttcccc tcttccccgt
     4861 tcccctcttc cccttttacc ccgccccccg caccgccccg aagaagcgtt ttaacttttc
     4921 ttcatccagg tggccttcgg ttcgcactcc gctgcaaaca tctactccaa agggacgtac
     4981 ctgcctgatc gcctccgcca cgttttctgc tttcaaccct ccggcgagaa agagcggcac
     5041 atcgatcgct tcgcgaatct gcacactcag gttccaatcg tgcgtccggc cagtacctcc
     5101 cagctctttt accggcaagg actgattgcc cgaatcaagc aatactgcat caacaaacgc
     5161 tgctactgag atcgcttcgg caactgaatc gacgtcatta acgtgaacca cctgcaccag
     5221 cgagattccc ggaagcgctg cgcgcagcgc gtcataactc tgtagctcca gccgatcaca
     5281 aatctgaatc gtattcacgc gcagccg
//
LOCUS       DASUEX010000659         4214 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_546635, whole genome shotgun sequence.
ACCESSION   DASUEX010000659 DASUEX010000000
VERSION     DASUEX010000659.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4214
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_546635"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1218
                     /locus_tag="VFV61_10955"
     CDS             <1..1218
                     /locus_tag="VFV61_10955"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HEX4901042.1"
                     /translation="KNINDSLGHIAGDQLLIATARCLEDCMRPMDTVARLGGDEFAIL
                     LDGLESVSDAILVAERIQQALTRPFTLNGHEVFVTTSIGITLSNTGYEDPENVLRDAD
                     TAMYRAKESGKARFEVFDSSMHSHAVALLQLENDLRRAIERREFKVYYQPIVNLDTDE
                     VSGFEALVRWYHPERGLVPPDEFIGIAEETGLIVDIGILVLRESCRQLREWQARPGLK
                     SLTVAVNLSAKQFAQPDLVSQVKEILLETGLDAQYLKLEITETVVMENAEMARNMLSQ
                     LCALGVQLSIDDFGTGYSSLSYLHRFPVTTLKIDRSFIGRMGANGENCEVVKTINTLA
                     NNLGMAVVAEGIETEDQLAQLKAMGCGYGQGYIFSRPLTTEAATLFVREHRALMLMDL
                     EDSLVQSELQLVN"
     gene            1542..2201
                     /locus_tag="VFV61_10960"
     CDS             1542..2201
                     /locus_tag="VFV61_10960"
                     /inference="COORDINATES: protein motif:HMM:TIGR02046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4901043.1"
                     /translation="MSRTFVLRKLHQLSGIVPLGLFLLEHFYTNSKALGGAAPFNEAV
                     KDLQAIPYILLVEIGGIFIPLIYHAVYGLVITMEARPNNLNYPYPRNWFYLIQRITGI
                     VLFFFIAFHVLNFRFGMIPRLNTISVAHRPDLAFDIVSGEFQIVPIFIIYLIGITATV
                     WHFANGIWLFTVDWGIAIGERAQRLTGYACIAFGAVLLLVGINAAIAFIRPGGLLGGL
                     L"
     gene            2205..4004
                     /gene="sdhA"
                     /locus_tag="VFV61_10965"
     CDS             2205..4004
                     /gene="sdhA"
                     /locus_tag="VFV61_10965"
                     /inference="COORDINATES: protein motif:HMM:NF006392.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HEX4901044.1"
                     /translation="MASNGIKIAIVGGGLAGLAAAMKIAEAGHNVDLFSVVPVKRSHS
                     VCAQGGINGAVNTKGEGDSTWEHFDDTVYGGDFLANQPPAKAMCEMAPSIIYLFDRMG
                     VPFSRTQEGLLDFRRFGGTKHHRTAFAGASTGQQLLYALDEQVRRFEVEGKVRKFEHW
                     EMLSLVLDDHQVCRGLVAMDLQTLELKAFPADAVIMATGGPGLIFGKSTNSMVCTGSG
                     VGACYQQGAKYANGEFIQVHPTSIPGEDKLRLMSESARGEGGRVWVPRQPGDARSPSA
                     IPEKERLYFLEEKYPAYGNLVPRDIATREIFQICIEGKGVGGDNQVYLDLTHIPAETL
                     DRKLGAILEIYEMFVGDDPRHVPMRIFPGVHYSMGGLWVDFNQRTNISGLLAAGECDY
                     SIHGANRLGANSLVSCVFGGFVAAPAAIEWAQNVERNGTDGSAIYDGELKRQQEINDS
                     LIKQSGSENQYILHQEMGKWMTDNVTVVRYNEKLKATDQKLLELMDRYKRISINDSNL
                     WATMALPHARHLDNMLELARVITLGALNRNESRGAHFKPEYPDRDDEQFLKTTIAEFS
                     GEGPVISYEPVDVSLITPRKRDYSKAKKETAKA"
BASE COUNT          997 a         1133 c         1118 g          966 t
ORIGIN      
        1 aaaaacatca atgacagtct gggacacatc gccggtgatc aactgttaat agccacggcg
       61 cgctgtctcg aagattgcat gcggccgatg gataccgtgg cgcgtttggg cggagatgaa
      121 tttgccatcc ttttggacgg cttggaaagt gtcagcgacg cgattctcgt cgcggaacgg
      181 attcagcaag cattgactcg ccctttcacg ctcaacgggc acgaagtttt tgtcactacg
      241 agcatcggaa tcaccttaag caatacgggt tatgaagatc ccgagaacgt cttgcgcgat
      301 gccgataccg ccatgtatcg ggcgaaagag agcggtaagg cccgcttcga agtcttcgac
      361 tcgtcgatgc actctcatgc ggtagcgctt ctgcaactgg aaaacgacct gcgccgcgcg
      421 atcgaacgac gcgaattcaa ggtttactac caaccaatcg taaatcttga cactgacgag
      481 gtctcgggct ttgaagcgtt ggtccgctgg taccacccag agcgcggcct ggttccaccg
      541 gacgaattca ttggcatcgc ggaagagacc ggcctgatcg tcgatattgg aattctggta
      601 ttgcgcgaat cgtgccggca gttgcgggaa tggcaggcaa ggccgggact caagtcgcta
      661 accgtcgccg tcaacctttc cgcaaaacag tttgcccaac cggatctcgt tagtcaggta
      721 aaagaaatct tgctcgaaac tggactcgac gctcagtatc tgaagctgga gatcacggaa
      781 acggtcgtca tggagaacgc tgaaatggcg cgcaatatgc tgagtcagct ctgcgcgctg
      841 ggtgtgcaac tcagcattga tgatttcggc actggctatt cttcgctaag ctacctgcat
      901 cgatttcccg tcacgacttt gaaaatcgat cgctccttca tcggcaggat gggcgctaac
      961 ggcgagaact gcgaagttgt taagacgatc aacactctgg cgaataacct gggaatggca
     1021 gtggtggctg aaggaattga gaccgaggat cagctggcgc agctcaaagc aatgggctgc
     1081 ggttatggac aaggctacat cttctcccga ccgctaacca ctgaagccgc cactctattt
     1141 gttcgggaac accgcgccct catgcttatg gacctcgaag acagcctggt gcagtcggag
     1201 cttcagctag tcaactagct cactgccgat tgccaagtgc cgattaccga tcgccaaaaa
     1261 tgatgaatga tgaacgatga acgatgaagt tgaaggaggt cagaaatcca ccttccgaac
     1321 tgcagtgttt atatagttaa gacaccttta ttgaaagaca gaattacgac aagaaatatt
     1381 catcattcat cattcatcat tcatccttgg ttttgcattt cctcaccgct tccttataat
     1441 tcaatcgttc tgggcaatct cttacacaac ccgcctgatt caagaccgcc ggcttcgctg
     1501 ctgaagcttt gcggcaaagg agtgcgtcct tggcgattac gttgagcagg acattcgtcc
     1561 tgcgcaagct gcatcagtta tctggaatag ttcctctcgg cctctttcta ctggaacatt
     1621 tttatacgaa ttcaaaggcg cttggcgggg cggccccgtt caacgaggct gtcaaggacc
     1681 tgcaagcaat tccctacatc ctgcttgtgg agatcggtgg catctttatt ccgctgattt
     1741 accacgcagt ttatggtctt gtaattacca tggaggcgcg tccgaacaat ctgaactatc
     1801 cctatccgcg aaactggttt tacctgattc aaagaattac cgggatcgtg ctctttttct
     1861 ttatcgcttt ccacgtactc aacttccgct ttggcatgat cccgagactc aatactattt
     1921 ccgtggctca ccgtcctgat ctcgcctttg acatcgtgtc tggcgaattt cagattgtgc
     1981 caattttcat catctacctg atcggaatca ctgccactgt ttggcatttt gcgaatggca
     2041 tctggctctt cacggtcgat tggggaatcg ccatcggtga acgagcacaa cgcttaacag
     2101 gatacgcgtg catcgctttt ggagcggtat tgttgctggt gggaatcaac gccgcgatcg
     2161 cattcatacg tccgggcggc ttgttgggag gacttctcta aacgatggca agtaacggaa
     2221 tcaaaatcgc gatcgtgggc ggtggtcttg ccggactggc cgccgcgatg aagattgccg
     2281 aagctggtca caacgttgac ctcttctctg tcgttcccgt aaaacgctcg cactcagttt
     2341 gtgcacaagg tggtatcaac ggcgccgtca acaccaaagg cgaaggcgat tccacctggg
     2401 aacatttcga cgatacagtt tacggcggcg attttctcgc caatcaacca ccggcaaaag
     2461 ccatgtgcga gatggcgcca tcgatcattt acctttttga tcgcatgggt gttcccttct
     2521 cacgcaccca ggaagggctg ctcgactttc gccgctttgg cggcaccaaa catcaccgca
     2581 cagcgtttgc cggagcttct accggccaac aattactcta cgcgctcgat gaacaggtac
     2641 ggcgctttga agtcgaggga aaggtgcgaa agttcgagca ctgggaaatg ctctcgcttg
     2701 tgctcgacga tcatcaagtt tgtcggggat tggtagcaat ggatctccag acgctcgagc
     2761 tcaaagcttt tcccgctgat gccgtcatca tggcgacggg agggccgggc ttaatcttcg
     2821 gcaagtcgac caactcgatg gtctgcaccg gcagcggcgt gggcgcctgt taccagcagg
     2881 gtgcgaagta tgccaacggt gaattcattc aagtgcaccc cacttcgatt ccaggcgaag
     2941 acaaactgcg cttgatgtcg gagtcggcgc gcggcgaagg tggccgcgtt tgggtgccac
     3001 gccaacccgg cgacgccaga tcgccttcag cgattcccga gaaagaacgc ctctattttc
     3061 tcgaagaaaa gtacccagcc tacggcaacc tcgtaccgcg cgatattgcg actcgcgaga
     3121 tcttccagat ctgtatcgaa ggcaaaggtg tgggcggcga taatcaggtt tatttggacc
     3181 tgacgcacat cccggcagaa acgctcgata ggaagctcgg cgcgatcctg gaaatctacg
     3241 agatgtttgt cggcgacgat ccgcgccatg tgcctatgcg gatcttcccc ggagtacatt
     3301 attcaatggg tggactctgg gtcgacttca atcagcgcac aaacatttcc ggtctgctgg
     3361 cagccggtga atgcgactat tcaatccacg gcgccaatcg tctcggcgcg aactcgctcg
     3421 tgagttgtgt ctttggcgga tttgtcgcgg cacccgcggc catcgagtgg gcgcagaatg
     3481 tggaacggaa cggcaccgat ggttcggcaa tctacgatgg cgaactcaaa cgtcagcagg
     3541 agatcaatga ttcgttgatc aagcagagtg gtagcgagaa ccagtacatt ctccaccagg
     3601 agatgggcaa gtggatgacc gacaacgtga cagtcgtgcg ctacaacgag aaactgaaag
     3661 ccaccgatca aaagttgctt gagctaatgg atcgctacaa acggatctcg attaatgact
     3721 cgaatctctg ggcgacgatg gcgctgccac acgcccggca tctcgataac atgctggagc
     3781 tagcgcgtgt gatcactttg ggcgcgctca atcggaatga atcgcgcggg gcccacttca
     3841 aaccagaata ccctgatcgc gatgacgagc agtttctaaa gactactatc gcggagtttt
     3901 ccggcgaggg accggtgatt tcctatgagc cggttgacgt gtcgctgatc actccgcgca
     3961 aacgcgacta cagcaaagcc aagaaagaaa cagccaaggc ctgagacttc aattatgtcg
     4021 gcacatccac aaccagcaat gcccaggcgc gttccgccca agaccgtcga gttacacatc
     4081 agacgccgcc ccaacccgga tgcgcctgct tattgggagc agtttgaaat tccctaccgg
     4141 ccaaacctga acatcatttc ctgcttaatg gagattcaaa agcatccggt gacgaaggaa
     4201 ggaaatgcca caac
//
LOCUS       DASUEX010000660         6758 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1714200, whole genome shotgun sequence.
ACCESSION   DASUEX010000660 DASUEX010000000
VERSION     DASUEX010000660.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6758
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1714200"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            160..235
                     /locus_tag="VFV61_10970"
     tRNA            160..235
                     /locus_tag="VFV61_10970"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:193..195,aa:Ile,seq:cat)
     gene            complement(271..1914)
                     /locus_tag="VFV61_10975"
     CDS             complement(271..1914)
                     /locus_tag="VFV61_10975"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX4901045.1"
                     /translation="MSLRVERSRTRAYRQGVLCLALFTIFSPSLEVRSQNVGRPDDLL
                     PAPRSNLVPVHWPDLTKLEAEVREQLMSLQSSLAKTVKTNSSEATLSNAYGAMAESYH
                     AYSLNSPARECYLNAAHLTPKEFRWVYLLGKLNEQEGRFNDAIRGYQIARGLRPDYAA
                     VPVNLGNIYLQLDRLEDAKENFNAALLIDENCAAALYGLGQVALSQRSYGQAINNFEK
                     ALSLIPAANRIHYSLAMAYRGLGNTEKATAHLAQQGSVGVRVNDPLVDGLQELIKGER
                     IHLIRGKLAVEARRYAEAGDEFRKAIAANRNSIPALVNLGAVLVLTGDLKGAADQYEE
                     ALRIDSTNANAHYNLAILLGKENKHEQAIVHLQAVLRLDTNDLSARFLLAGELRKSER
                     LEEALAEFSRVALADPDNEAALLEQVKLLQRMGQYQQALESLEKGHARNPQKGQTIEM
                     LAYLLAASPRYDLRDGARALHFAQLVFKQTGSLPHGALVAMALAELARCDEAAVWQRK
                     LIAVAEQEHKVDLLSKLRMDLRQYEKGQPCRPQGETASQ"
     gene            complement(1911..3671)
                     /locus_tag="VFV61_10980"
     CDS             complement(1911..3671)
                     /locus_tag="VFV61_10980"
                     /inference="COORDINATES: protein motif:HMM:NF019229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HEX4901046.1"
                     /translation="MEKKFIVLLVLIIGHCYVPFRLAAQRQPARATASSLPIFKEVAE
                     QVGLKFQHYNGMTGKLYLPEIMGSGAAVFDFDNDGDLDVFLVQGNVLEPGTQPASTQF
                     PWRGPGLPRSRLFRNDLVVGKDGSRTLKFTDVTEKSGIRADGYGMGVAVGDINNDGWP
                     DLYITNLGRNQMYLNKGDGTFSEVTGKTGTDDPRWSTSAAFFDYDRDGWLDLMVVNYA
                     NFSATNSPTCYAATSARDYCTPRVFRAPGNRLFHNKGDGTFEDVTVGTGVDKEFGHGL
                     GVVTADFDGDGWTEIYIANDGDPNQLWINQKNGTFKNEALLAGAAVNRDGKAEAGMGV
                     DAGDFDGNGTEDIFVTHLMDETNTLYVNLGKALFEDRTREASLGMPGRRFTGFGTAFF
                     DYDNNGWLDLLVVNGAVQLLPELMRKGDPHPLGQPNQLFQNDGRGKFVEVTDNVAVGF
                     QLLEVSRGAAFGDLDNDGDTDVLVTNNNGPARLFLNQVGSRNHWLGLRLISKNAGRYA
                     LGAQVEIVISKNNFLWRRVRTDGSYLSANDPRVLVGLGGAAAVETVRVRWPDGTVEEW
                     KGIPVDQYITLQQGTGSQKQ"
     gene            3831..4934
                     /locus_tag="VFV61_10985"
     CDS             3831..4934
                     /locus_tag="VFV61_10985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4901047.1"
                     /translation="MFPRLSPAVCLLIGCLAAVPTLSQSGPRDRKVAGAFAPVFYQAL
                     GDKPRSDYITNFDFDGDWRGDNNWDHADDKKFPLKAYVYYSVSETATHFFIHYAIFHP
                     RDYKGGESKGRILSDLIREGVKGGTEHDPTGLLEEAGVAHENDLEGCLIVAAKDGNDL
                     KRAKVVFVETLHHNDFSLYVPGVTAPKGFGLFRSEDGHPLLYVEPKGHGVKTYSGDEK
                     QTAKKEFLIYKFKGKAEDPEQEHDGSVGYDLLPIKTTLWARALNGVGNGANLVYGSTH
                     NYGQIAINVVGANGIVVSRKITVGKVGSAFFGNTGGRNMARPPWAWFDNNAREQPLGL
                     WFFDPATIVKRDFRAGESFSTAYIRLPFWASSR"
     gene            complement(5142..6104)
                     /locus_tag="VFV61_10990"
     CDS             complement(5142..6104)
                     /locus_tag="VFV61_10990"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX4901048.1"
                     /translation="MNRRRMLTQLCAAFIGAQFALGQNRPDKIATEDLSKQVSAPGVQ
                     TLLTGLGFGESPRWHKDRLWFSNWGKQEIVAVDYKGKSEVMVRLPFATFPFSIAWLPN
                     GSLLIVSTSERPLLRQQKDGSLVSHADLSRLPAQGWNEIVVDGRGNTYINGGDFGAKN
                     SASGIIALVAPDGSVRQVADGLAFPNGMAVTANNSTLIVAESFGKRLTGFDIAANGSL
                     SNRRVWADLKEGVPDGISADAEGAIWYADVPNKRCVRVREGGEVLQTVNLDRGCFACM
                     LGGADRKTLYILAAEWRGFEKMMEGLGTGQLLTVKAPAQGVGWP"
     gene            6304..>6758
                     /locus_tag="VFV61_10995"
     CDS             6304..>6758
                     /locus_tag="VFV61_10995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4901049.1"
                     /translation="MRTRLGVFLITLSGLVLEVGLTRIYSASVWYHFAFVAISVALLG
                     WGLGGFTVHLLKRKMRLTMNAAALVAAVYSLTIPLCLWLLNRFPFEVDRLPLYFLTPL
                     IPFFLAGMALAIVFDLHRTEAGLLYFADLLGAAIGAVLVTLLLHWLGGEP"
BASE COUNT         1416 a         1927 c         1768 g         1647 t
ORIGIN      
        1 taactatgaa ttaccgttga gtttcaagag actggtccga ctacgattga ctcttggccg
       61 ccactaacaa agccttgcaa attggcaatt ggaaattggc aatagcggtc gtgatacact
      121 gattaaacaa ttcgactaag acggttaata cgctcgtgtg cccctgtagc tcaacggtta
      181 gagcagcaga ctcataatct gttggttcgg ggttcgaatc cctgcggggg caccaatcaa
      241 atgcggttgc gtagaagttt gtttgggatt tcactgactg gcagtttcac cctgcggccg
      301 gcacggttgt cctttctcgt attgcctcaa atccatcctc agttttgaca gcagatccac
      361 tttgtgttcc tgctcggcca cggcaatcag ttttcgctgc cacacggctg cttcatcaca
      421 gcgcgcgagt tcagccaaag ccatcgcgac cagcgcaccg tgcggcaatg atccggtttg
      481 cttgaacacc agttgagcaa aatggagcgc ccgggcgcca tcacgtaggt cataccgtgg
      541 cgacgcggcg aggagatatg cgagcatctc aatagtttgc cctttctggg ggtttcgcgc
      601 gtgacccttt tccagactct ccaacgcttg ctggtactgc cccattcgtt gcagcagttt
      661 cacctgttcc agcagcgctg cttcattgtc gggatcggcc agagccaccc gcgagaattc
      721 tgcgagcgcc tcttccaggc gttcggattt ccttaactct ccagccagta agaaccgcgc
      781 gctgagatcg ttcgtgtcaa gcctgaggac ggcttgcagg tggacgatag cctgctcatg
      841 cttattttcc ttgcccaaca gaatggccag attgtaatgc gcattggcat ttgtggaatc
      901 gatacgaaga gcttcttcat attgatcggc cgcgcccttt agatccccgg tcaagacgag
      961 tacggcaccc aggttcacga gcgccgggat actgttacgg tttgccgcaa ttgccttgcg
     1021 aaactcgtca cccgcttctg catatctccg cgcctcgacg gccagcttgc cgcgaatcag
     1081 gtggatacgc tcccccttga tcaactcctg cagtccgtcc accaacggat cgttaactcg
     1141 cacgcctacg gaaccttgtt gcgccaagtg ggccgtagct ttctccgtgt tgcccagccc
     1201 gcgataggcc atggccagag aataatgaat tcgatttgcg gcaggtatca gcgacaatgc
     1261 cttctcaaaa ttgttgattg cctgaccgta acttcgctgc gacagagcga cctgtcccag
     1321 cccataaagc gccgccgcac aattctcgtc aatcagaagc gctgcgttga agttctcttt
     1381 cgcatcttcc agccgatcca gttgcagata gatgttaccg agattcaccg gcacggcagc
     1441 gtagtccgga cgcaaaccac gcgcgatttg gtagccgcgt atcgcgtcgt tgaagcgacc
     1501 ttcctgctcg ttcagctttc ccagtagata gacccagcga aactctttcg gcgtcagatg
     1561 cgcagcgttc aggtagcact cacgggccgg agagttgagc gaataagcgt ggtaactctc
     1621 tgccatggca ccgtaagcat tgctcagcgt cgcttcactg ctgttagtct tcaccgtttt
     1681 ggcgagcgag gattgtaacg acatcagttg ttcgcgcact tcggcttcca acttcgtcaa
     1741 gtccggccaa tgcactggga cgaggttgga cctgggagct ggcagaagat cgtcaggccg
     1801 accgacgttt tgcgagcgaa cttcgagtga gggcgaaaag atagtgaaca gcgccaggca
     1861 aagtactccc tgtcgatagg cgcgagtcct gcttctctcg acgcggaggc tcattgcttc
     1921 tgcgaccccg tgccttgctg caacgtaatg tattgatcaa ctggaatacc tttccattcc
     1981 tcgaccgtgc cgtcgggcca gcgcactcgc acagtttcca ccgcagcagc accgcccaat
     2041 cctacaagca cgcgcggatc atttgccgat agatagctgc cgtcggtcct gacgcgccgc
     2101 cacaggaaat tgttcttcga tatcacgatc tctacttgcg cacccaacgc gtagcggccg
     2161 gcgtttttgc tgatcagccg cagtcccaac cagtggtttc ggctgccgac ttgattcagg
     2221 aacagcctcg ccggcccatt gttgttggtc accagtacgt ccgtgtcgcc atcattgtcg
     2281 aggtcgccaa aggccgcccc gcgacttacc tcgagcaact gaaagccgac ggcgacatta
     2341 tctgtgacct cgacaaattt cccccttcca tcgttctgga agagctggtt cggctgaccc
     2401 agcgggtggg gatcgccctt acgcatcagc tccggcaaca attggacagc cccgttgaca
     2461 actaagagat cgagccagcc attgttgtca tagtcaaaga acgccgtgcc aaaacctgtg
     2521 aaacgacgcc ccggcattcc cagacttgcc tcgcgcgtac gatcctcaaa aagggcttta
     2581 cccagattca cgtaaagcgt attcgtctcg tccatgaggt gggtgacaaa gatgtcttcg
     2641 gtgccattgc cgtcgaaatc tcccgcatcc acacccatgc cggcctctgc ctttccatct
     2701 ctgttgacgg cggcgccggc caggagtgct tcgttcttaa aggttccgtt cttctggtta
     2761 atccagagct gattgggatc accatcgttg gcaatgtaaa tttctgtcca gccgtcacca
     2821 tcaaaatccg cggtgaccac acccaatccg tggccgaact ctttgtccac gcctgtgccg
     2881 acagtgacgt cttcaaatgt accgtcgcct ttgttgtgaa acaggcgatt gccgggtgca
     2941 cgaaaaacgc gtggtgtgca atagtctctc gcgctggtgg cggcgtaaca agtgggactg
     3001 ttcgtcgcgg aaaaattggc gtagttcact accatcaaat caagccagcc atcccggtca
     3061 tagtcgaaga aggcggcgct cgtactccag cgtggatcat ccgtcccggt ttttccggtg
     3121 acttcggaaa acgtcccgtc acctttgttc aggtacatct ggtttcgacc gagatttgta
     3181 atatacagat cgggccagcc atcgttattg atgtcgccga ctgcgacgcc catcccgtat
     3241 ccgtcggctc ggatgccact cttttcagtt acatcggtga acttgagtgt ccggctgccg
     3301 tccttgccga ccacgaggtc gttgcggaag agccggctgc gcggtaaccc cggaccgcgc
     3361 cacggaaact gcgtactcgc tggttgcgtt cccggttcca gcacgttgcc ctgtacgaga
     3421 aaaacgtcca ggtcgccatc gttatcaaaa tcgaaaacgg ctgctccact gcccatgatc
     3481 tcaggcaaat acagttttcc agtcatgccg ttgtagtgtt ggaacttgag tcccacctgc
     3541 tccgctacct ccttgaagat tggtaacgag gacgctgtcg cacgggctgg ttggcgttgg
     3601 gctgccagcc gaaacggcac gtaacaatga ccgataatga gaactaggag gactatgaac
     3661 tttttttcca tgagtatgag agctaattat tattccattc gaatgtgtgc gcaaacttga
     3721 cccttcaaca ggtctcataa tctgttggat tggattgagg tcaatgccgt ctgcgctagc
     3781 gtcagttctg cgcaaatcct gcctacgcac atctttgata tgatgcgtca atgttccccc
     3841 gactttcgcc agcagtttgt ctcctaatcg gctgcttagc tgcggtgccg accttgtcgc
     3901 agtctggtcc ccgtgatcgc aaggtagcgg gcgcgttcgc gccggtattt taccaggcac
     3961 tgggcgacaa accgcgcagc gactacatca ccaattttga tttcgacggc gactggcgcg
     4021 gcgataataa ttgggaccac gcagacgaca agaagtttcc tctcaaagct tacgtttact
     4081 attcagtttc cgagaccgcc actcattttt tcattcacta cgccatcttc catccgcgcg
     4141 actacaaagg cggcgaaagc aagggtcgta ttctgagcga cttgattcgc gaaggcgtga
     4201 agggtggaac tgagcatgac ccaactggct tgctggaaga agcgggcgtg gcgcacgaga
     4261 acgacctgga gggttgtttg atcgtcgcgg cgaaggatgg caacgatctc aagagggcga
     4321 aagttgtttt tgtcgagaca ctccatcaca acgacttttc cctttacgtc ccgggcgtaa
     4381 cggcgccaaa agggttcgga ctctttcgct cagaggacgg ccaccccctt ctgtatgtcg
     4441 agcctaaggg ccatggtgtc aagacatatt caggcgacga aaagcagaca gcgaaaaaag
     4501 aattcttgat ctacaaattt aagggcaagg cggaagatcc ggagcaggag cacgacggtt
     4561 ctgttggtta cgatctgctt ccaatcaaga cgacgttatg ggcccgggct ctaaatggag
     4621 tagggaacgg tgccaacctc gtgtacggca gcactcacaa ctacggacag attgctatca
     4681 atgtggtggg tgcgaatgga atcgttgtca gccgtaagat aacggtgggg aaagttggct
     4741 ccgctttctt cggcaacacc ggcggccgca atatggcgcg accaccgtgg gcctggtttg
     4801 acaacaatgc ccgcgagcag cctttgggct tgtggttttt tgatccggcg acgattgtca
     4861 aacgcgattt ccgggcaggg gaatctttta gcacggccta catacggctg cctttctggg
     4921 caagctctcg ttagccactc tcttatccaa aatactgtgc gtagatctgt tgaaacgtcg
     4981 gcgagtgcgt gtgagctaac cgcgcgacgt ctgagttcga gtctctgcgg gtcagcaaat
     5041 aaattgtcgc ttgccaatct tgaggttccg gaatcgctat tgaaaatcgc cgtgctggat
     5101 tgcagtcaac gacactatag tggttacctt ccggctgctt ttcatggcca cccgacgccc
     5161 tgggccggcg ccttaactgt gagtaactga cccgttccca gtccttccat catcttctca
     5221 aatccgcgcc actcagcagc cagtatgtaa agtgtcttcc gatctgctcc gccgagcatg
     5281 caagcgaagc aaccgcggtc gaggttgact gtttgcagca cctcgccccc ttcgcgcacg
     5341 cgcacgcaac gcttgttggg aacgtcagcg taccaaatgg caccttcagc atccgcgcta
     5401 atgccatcag gaacaccttc tttaaggtcg gcccacactc gccgattcga tagacttccg
     5461 tttgcagcta tgtcgaaacc agtgagtctc ttgccgaacg actcagcgac gatgagggtt
     5521 gagttgttgg ccgtcaccgc catgccattc ggaaaggcaa gaccatccgc tacctgacga
     5581 acagagccat cgggagcgac gagcgcgatg attccggagg cagaattctt agcaccaaag
     5641 tcgccgccat tgatgtaggt gttgccgcga ccatccacaa cgatctcgtt ccaaccctgc
     5701 gccggtagcc ggcttaaatc ggcatgagat accagcgagc catctttttg ctgacgcagc
     5761 agcggccgtt cgcttgtcga cacgataagc aaacttccgt taggcagcca ggctatggaa
     5821 aacggaaacg tcgcgaacgg cagtcggacc atcacctcgc tcttgccttt gtaatcaacg
     5881 gcgacgatct cttgcttgcc ccagtttgag aaccacagcc ggtctttgtg ccagcgcggc
     5941 gattcgccga atccgaggcc ggtgagaagc gtttgcactc ccggtgccga cacctgtttt
     6001 gacaaatcct cggtagctat tttgtctggt cggttttgtc caagtgcaaa ctgtgcaccg
     6061 ataaaggcag cacacagttg ggtaagcatc cttcttcggt tcattggttc ctccttgcga
     6121 acctggctta ttaatcccgc gtcggtccaa gtccactcca gcgtgttcct atcagtatgt
     6181 cgtttgagag tcgtcggttt cgacacccga ccagatattt cttcatactg attccagccg
     6241 atgagtgcac tgcgagtaga attagcttga cgttcgtgcc cgcttccctt ttactaggcc
     6301 gccatgcgaa ctcgcctcgg cgtttttctt atcaccctat caggacttgt acttgaagtg
     6361 ggactcactc gcatttactc cgcgagtgtc tggtaccatt ttgctttcgt tgccatatct
     6421 gtcgctctgt tgggatgggg actgggaggt tttacagtcc acctgctgaa gcgaaagatg
     6481 cggctcacga tgaatgcggc ggcgctggtc gccgctgttt actcgcttac gattccgctt
     6541 tgtttgtggc tacttaatcg ctttcccttc gaagttgacc ggttgcctct atactttctg
     6601 acacccttaa ttcctttctt tctggccggc atggcattgg cgatcgtctt tgatcttcat
     6661 cgtactgaag ccggcctttt gtactttgcc gatttattag gagccgcaat tggagcagtg
     6721 ttagttacat tgttgctgca ctggctcggg ggtgagcc
//
LOCUS       DASUEX010000661         2013 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_673625, whole genome shotgun sequence.
ACCESSION   DASUEX010000661 DASUEX010000000
VERSION     DASUEX010000661.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_673625"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2013
                     /locus_tag="VFV61_11000"
     CDS             <1..>2013
                     /locus_tag="VFV61_11000"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEX4901050.1"
                     /translation="KDELFHFTHPRKLLYALRRHREIAYLGDDDRAQLDRADKLLRKL
                     LERRNQYPIAELLRLAVAEAEYLSVIAANFDGAQKLANVRKLFTLAERFEHAGAHLVR
                     DFVRYVEDFEAIGSREGEGQIDDSANAVRLMTIHQAKGLEFPVVIIPNLHHRTLKPQE
                     HWYALDRHRGLTVKIPDGRGKQVAGCTLERFRARNQLREHFESVRLLYVAATRAQNRL
                     ILAGVTESLDKLKNSPDNWLKLIWQKLELQVSRSGVVDLDPTTQLQVTLNSVADSLID
                     LAPRLSPLAASLDVSSEKETVASLTSAFPLLQPIAPVLEPQFAGQRLSVTQLINYERC
                     PRQYYFDRVLRIPSADELAIWNNAEAPEPPANLNATLKGAVIHRFCETYTPGDDVQKR
                     LAESFSDLIRTRQSQLADRLEDINVSEALKELSPLAENYLASEVFQRAERARRIVGDE
                     LTRLPAGEAGLWSELTFRLRRPWGILTGTIDKLLITPSANGAGFEVEIIDFKTNRITA
                     PAPQASGARVQAAAVAPPALLAPMQRGKRIRSSDSGAQFAFEFSSAEPAPQIATDPAV
                     ASTMSELVSLAASDYRLQMQAYALAVHELLPALSAATIRVTLHFLHPNVEWMLPAESL
                     TPEACVAAIDGAMKKIICSVSPEDYPVRPARHCGMCAFLRICAAGRE"
BASE COUNT          438 a          560 c          551 g          464 t
ORIGIN      
        1 aaggacgaac tgttccattt cactcatccg cgaaaacttc tttacgcctt gcgccgccac
       61 cgagagattg cgtacctcgg cgacgatgac cgggcgcaac tcgatcgggc cgacaagctg
      121 ctgcgcaagt tgttggaacg acgcaatcag tatccgatcg ccgagttgct ccgtctcgcc
      181 gtcgccgaag ctgagtattt atcggtgatc gccgccaact ttgacggcgc gcagaagctg
      241 gcgaatgtta gaaaactgtt cacactcgcg gaacgcttcg agcatgccgg tgctcatctt
      301 gttcgcgatt tcgtgcgcta cgtggaagac tttgaagcta ttggcagtcg tgaaggcgaa
      361 gggcaaatag atgattccgc gaatgcggtg cgcttgatga cgattcatca ggctaaggga
      421 cttgagttcc ccgtggtcat cattcccaac ttgcaccatc gcaccctcaa gccacaggag
      481 cattggtacg ctctggaccg gcaccgcggt ctgactgtga agattcccga tggccggggg
      541 aagcaggtgg cgggttgcac gcttgaacgt tttcgcgccc gcaatcaact tcgggaacac
      601 tttgagagtg ttcgcttgct ttatgttgcc gccacccgcg cccagaatcg cctcattctt
      661 gccggcgtta ctgagtcgtt ggacaaacta aagaactcgc ccgacaactg gctcaaatta
      721 atctggcaaa aacttgagct gcaagtctcg cgcagcggcg ttgtcgatct cgacccaaca
      781 acacaactgc aagttaccct caactcggtt gctgactcgt tgatagatct cgcgccaaga
      841 ctttcgcccc tggctgcaag cttggatgtt tcctccgaaa aagaaaccgt tgcttcgtta
      901 actagcgctt tccctttact gcagcctatc gctccagttc tggaacctca atttgctggg
      961 cagcgcctga gcgtaaccca actaatcaat tatgagcgtt gcccgcgcca gtactatttc
     1021 gatcgcgtgt tgcgaattcc ttcagccgac gagctggcaa tttggaacaa cgctgaggcg
     1081 ccagaacctc cggcaaatct gaacgcgact ttgaaagggg cagtaatcca ccgcttctgc
     1141 gaaacctaca ctcctggtga cgatgttcag aaacgcctgg ctgaaagttt ttcggatctg
     1201 atccgtacgc gccagtcgca gctggctgat cgacttgaag acatcaatgt ttctgaggcg
     1261 ttgaaagaat tatcaccgct ggcggagaac taccttgcga gtgaggtttt ccagcgggcg
     1321 gaacgagcac gccgaatcgt cggtgacgag ctaacgcgtt tgcctgccgg cgaggcgggc
     1381 ctttggagcg agttaacctt ccgcttgcgg cggccatggg gaatcttaac tggcacaatt
     1441 gataagttgt tgatcacacc gtcggcgaat ggcgccggtt tcgaagtgga gattatcgac
     1501 ttcaagacta atcggatcac tgcaccagca ccgcaggcga gtggagcgcg ggtccaggct
     1561 gccgcagtcg caccacccgc gttgctagcg ccaatgcagc gcggaaaacg tattcgatca
     1621 tctgactcag gggcgcaatt tgcatttgag ttctcgagcg cagaaccggc accacaaatc
     1681 gcgacggacc ctgcagttgc ttcgacaatg tcagaattgg tgagtcttgc cgcctccgat
     1741 taccggttac agatgcaggc ctatgcgttg gcagttcatg aattgttgcc ggcgttgtcg
     1801 gcagccacga ttagggtaac gcttcacttt ctgcacccga atgtcgaatg gatgttgcca
     1861 gctgaatcat tgacgccaga agcttgcgta gccgccatcg atggagcgat gaaaaaaatt
     1921 atctgttctg tcagtccgga agactatcct gtccgtcccg cgcgtcattg tggcatgtgc
     1981 gcttttctgc gcatctgcgc ggccgggcgt gaa
//
LOCUS       DASUEX010000662         8728 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1715145, whole genome shotgun sequence.
ACCESSION   DASUEX010000662 DASUEX010000000
VERSION     DASUEX010000662.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8728
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1715145"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="VFV61_11005"
     CDS             <1..546
                     /locus_tag="VFV61_11005"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX4901051.1"
                     /translation="VRLNRPADRNPLSISTLTELSSSLPQWFSHDDIAAIIFTGADDI
                     FASGANIRELATLDLNAAKQFSEMGQRLLNSISQARPKTVAAINGHCIGGGLDLALAC
                     DIRIASANATFAHPGARLGIITGWGGTQRLPRLIGKSRALELFATARTLDAPAALRIG
                     LVDRIADPVLETAIQHAAVAP"
     gene            914..4150
                     /locus_tag="VFV61_11010"
     CDS             914..4150
                     /locus_tag="VFV61_11010"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEX4901052.1"
                     /translation="MKYALRLFMILCMGFAAATAFAQEPIGSIEGTVTDPQNAVVPNA
                     SVTVRNIATNLTRTTTAGDNGTYRISQLPPGTYEVKASGTNFKTSLISDVVVAVGQVL
                     PLDIHLEIGVPDETVTVVGGGEAQIERTDNTVSGVVNTRQIQNLPLNGRNFLDLAQLQ
                     PGVETVQGGSFDPTKANYTGISIAGQAGRSTQIAVDGASVVDNVVGTTTQNFSQEIVQ
                     EFQLGISNYELSTGASSTGSVNVISRSGSNEFHGNGYIYARSNKWAAFPALARLDAAN
                     GIPDSAQATEVPFDRQQFGGTLGGPIRKDKLFFFTNYEYNNQDGSSIHLPLIAPSFAG
                     FTASPFNETLFTTKVDWNMTSKMNSYFRYSFNDNDNNVPFPPGTGIVPRTSTSGIFES
                     NNQVVLNKTHGFVFGLTRAFSPNISNSFVYSLNDFHNRVDPVVPDIPEIRTVNDGQTF
                     RSGTNAITPQVTDQSRNQIRNDLTWVRGNHTFRFGGNYERTSITGLFVFANPVRIRIF
                     EPLGNGATENDFLDAPVFDISYGIGNATLPFNTPGGATVNHRFQFYGSDNWKLTKRFT
                     LNYGLAYRVDTNLWNHDQSRPAIVAGVFGKGTAPSPRDNNNIAPRLGFAWDVAGNGKT
                     VIRGGAGIYYDNTIDNLRLFERADLGPPGAQLFLVGFDVNSPLLPGDGDGRFSTNPAS
                     ASGFITLRDMLALAPSVRADIESRAFNCTLPTSIECFDAISGPLFSTEFQVPYSIQYS
                     VGFQRELPGNMVVQADYNYRKGLHEVIIYDANRLFDLTIPGQQRTAFPNTIPYADSSA
                     FSTYQALLVRLDRRFSKGFQMTASYSLSRFKAFGGDSLGLGVVITDQNDFRKEFGPGG
                     LDRTHRLVVSGLWDLPFFKKSDSWAKRNLLGNWQVSMISTAFSGLPQQAFLPHFADLS
                     RTGTFNSYLPGTGPGSLGRGIRSVNELNDIITAYNNSIPTLGEPCSLSISSTGRCDSN
                     DPSYRDPLERLALLPANQPIGGDSIISQDLRVTKTFDISETVKLDLIGEVFNLFNVAN
                     LTGVTDFILPDEGTPPDQITFLRPAQRSTSVFGTGGPRAFQFAAKFRF"
     gene            4270..5283
                     /locus_tag="VFV61_11015"
     CDS             4270..5283
                     /locus_tag="VFV61_11015"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX4901053.1"
                     /translation="MSAQIKRFVFVSIIVLSCCASLLAQGHSIRGKVRNAAGVNVGRS
                     PVTLERNGAMVEQTVTNNEGDFTFSGLTDTSYTVIVTAVDYNPASESVEFVRAAASDQ
                     PGELRTVEITLTSREPVRPPRAGINFVQNIPAPALAAFEAGLKHSRENRPTEAIASYR
                     EAIKIFPNYFNANLTLAAELAKQGQLQEAITSLEAARAANPKDDRVYDLFARVMMQQH
                     KYSVAARIYAEAARLNPAETQYLLARGTALVEQVVVIDGAPSTAAADERSFALAEAEG
                     VLSQAVKQNEKLADAHLQLARVYEKKGERARAAEQLERYLRKAPNAKNAEQIKAAIKT
                     LRQ"
     gene            complement(5392..6165)
                     /locus_tag="VFV61_11020"
     CDS             complement(5392..6165)
                     /locus_tag="VFV61_11020"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX4901054.1"
                     /translation="MPEENQMSATIYSVAGSIATIKLNRPDKRNALNDELIAGLKKGL
                     RAAAAEPSVRAVVISGAGKDFCSGADLSALRKISEASIAENAADARSMMDLFTLMREL
                     EIPVIAAVRGRALAGGCGLASAADLVLAAESARFGYPEVKIGFVPAMVMAILKRNVSE
                     KQAFELITRGGEITARRAYEVGLVNQVFADETFDSEVQAYVGVFAEMSRSAVALTKSL
                     LYQIDGMAFREAIETGADVNVIARLGEDCQQGIAKFLKK"
     gene            6458..7147
                     /locus_tag="VFV61_11025"
     CDS             6458..7147
                     /locus_tag="VFV61_11025"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF018649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEX4901055.1"
                     /translation="MTANPKPFTQAKLIELISRVVPSTFFLFVVMLKIAELYASIITH
                     QATNQPTSAKFIVEVCSRASTAAFLGLMTILFLIRLEPVEKAKSLLPRVMAIAGTFFV
                     ALVTLFPRAELSLTQSLVATSISIVGTILSIIALAHLGRSFSLMAEARRLVTTGPYRV
                     VRHPLYLFEEVASFGILLQFLSVTTALIFVIHILIQVQRMKNEEKVLEKAFPEYQAYK
                     ATTARVIPKLY"
     gene            7240..>8728
                     /locus_tag="VFV61_11030"
     CDS             7240..>8728
                     /locus_tag="VFV61_11030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4901056.1"
                     /translation="MPQAHKINWKWGVLAALVITLLALIPQFFMWGTRGSQWNGSYAE
                     LHGDEWVYSAYVQALIDGRPRRSDAYTGRDERPGHPEPESLFSIQFVPAYLIAMPARA
                     LGISSSTAFLILGLLAPCLSCLAIFWLFVNLTQDEKLASAAALVVLSFGALAAGEGLV
                     HLFISGSNYSFLPFLRRYEPVAPFPLFFVFCTLTWKSLNDRNKSLVWAGIAGVCLALL
                     IFSYYFLWTPALAWLICLAFLWLISNRAELRRGIPVFAIILLSAIAALIPYFILVSNR
                     SHTMDAGQKLVLSHAPDLFRVPELLGLAVFTLIAIAAKTGRVDWRAPATLFAASFALT
                     PLLVFNQQIITGRSLQPFHYEWFIANYLALSGLVVIAILLWRGRDQARPIRYRVAGRI
                     VFVALWWAAIEVLAPGKVIIRDSQFTDRAAAVCQRLRELSASERGTTSELDPLPIVLA
                     SENKVAVILPTFTSHAVFWAPHFDFLHLAPGESRARFYQYLYYTGV"
BASE COUNT         1903 a         2575 c         2220 g         2030 t
ORIGIN      
        1 gtccgtctca atcgacctgc tgatcgaaat cctctctcaa tctcaactct caccgagctc
       61 agcagctccc tgccccagtg gtttagtcac gatgatatcg cagcgattat tttcacgggt
      121 gctgacgaca tcttcgcctc aggagcaaac attcgcgagt tggcaacgct cgatctgaat
      181 gctgcaaagc agttttccga aatgggtcag aggctgctga attcgatttc ccaggcgcgg
      241 ccaaaaacag ttgctgccat caatggtcac tgcatcggtg gtggactcga cctcgctctg
      301 gcatgcgata tccgcatagc atcggcgaat gcgaccttcg cccatcccgg agcccggctc
      361 ggaatcatta ctggttgggg cgggacgcaa aggctgcctc gtctgattgg gaagtcgcgc
      421 gcgcttgaac ttttcgccac tgcgaggacc ctcgatgccc cggcagctct ccggatcggg
      481 ttagttgatc ggattgccga tccggttttg gaaactgcaa ttcaacacgc cgcggtggct
      541 ccgtagtgtc ggcacacgga gtcaacacaa ttccacaaat tcggaatgca ttccgagaat
      601 ttcgtaattc gacgaaatat cgttcttgaa atttctgaac tctcgtcagc attttggatt
      661 tttctttgtc gccttctcac acctcttcat agctaaacga tagctaaacg cagtaatagc
      721 agcggttgat ctcttgttcg cagagtgggc acgacgtttg cgaaaacgga tttcaggttc
      781 tctccccacc ttcccgacag gtctccctta ggtcttcccg acttcagtct ccctggaact
      841 gatctccccg gacacgactg ccgcaacccg gaactacgcg gcgttttctg gcctcagaaa
      901 aaggagcagc aacatgaagt acgctttaag gttgtttatg atcctgtgca tgggcttcgc
      961 cgctgcgact gcctttgctc aagagccgat cggctcgatt gaagggaccg taaccgatcc
     1021 tcaaaatgcc gtggtgccca atgcgtcggt tacagtacgc aacatcgcca cgaacctaac
     1081 tcgcaccacc actgctggcg acaacggcac ctatcgaatt tcgcagcttc caccgggaac
     1141 gtacgaggtg aaagcaagcg gcaccaactt caagacatcg ctgatttcag acgttgtggt
     1201 ggcggtcggg caggttctac cgctcgacat ccatcttgaa atcggcgtcc ctgacgaaac
     1261 agttacagtt gtagggggcg gtgaagcgca gatcgagcga accgacaaca ctgtttcggg
     1321 cgtggtcaat acgcgacaga tacagaacct gccactgaat ggacgaaact ttctagatct
     1381 cgcgcagttg cagccgggcg tcgaaacagt gcagggtggt agctttgatc ccacgaaggc
     1441 taactacact ggtatctcga ttgccggtca agccggtcgt tcgactcaga tcgcggtgga
     1501 tggcgcctcg gtcgtagaca atgttgtcgg cacgaccacg caaaacttct cacaggagat
     1561 cgtgcaggag ttccaactcg gcatctcgaa ctacgaactt tcaaccggcg cttcgtccac
     1621 tggctcggtg aacgtcatct cgcgctccgg ctcgaacgaa ttccacggca atggttatat
     1681 ctacgcccgc agtaacaagt gggcggcctt tccagcgctc gcgcgccttg atgctgccaa
     1741 cggcatccca gacagcgcgc aggcgaccga ggtcccattc gaccgtcagc agtttggcgg
     1801 cactttgggt ggccccatcc gcaaggacaa actgttcttc ttcacgaact acgagtacaa
     1861 caaccaggac ggctcttcta ttcatctgcc gttgatagcg cccagcttcg cgggctttac
     1921 cgcgagcccg ttcaacgaga cgctcttcac gacgaaggtc gactggaata tgacctccaa
     1981 gatgaacagc tacttccgtt actcgttcaa cgacaacgac aataacgtgc cgttcccgcc
     2041 gggcacgggc atcgtgccga ggacttccac cagcgggatc ttcgagtcaa acaatcaggt
     2101 agtactcaac aagacgcacg gatttgtatt cggcttgaca cgtgctttct cgccgaacat
     2161 atcaaacagt ttcgtctaca gtctgaacga ctttcacaac cgcgttgatc cggtcgtgcc
     2221 tgacataccg gagatacgca ccgtcaatga tggccaaacc tttcgctcgg gcacgaatgc
     2281 catcacgcct caagtcactg accaaagccg caatcagatt cgcaatgatc tgacttgggt
     2341 ccgcggcaat cacacgtttc gcttcggcgg caactacgag cgcacctcga taactggtct
     2401 gttcgttttt gcgaaccccg ttcgcattcg catcttcgaa ccccttggca atggcgccac
     2461 cgagaacgac ttcctcgacg ccccggtgtt cgatatctcc tatggcatcg gcaacgccac
     2521 gctgccgttc aatacgccgg gcggagcaac cgtcaaccac cgctttcaat tctacggtag
     2581 cgacaattgg aagctcacca agaggttcac actcaactac ggtctcgcgt accgcgtcga
     2641 caccaacctc tggaaccacg accagtcgcg tcctgcgatt gtcgctggtg ttttcggcaa
     2701 gggtactgcg ccgagcccga gggacaacaa caacatcgcg ccacgtcttg ggtttgcttg
     2761 ggacgtcgct ggcaatggta agactgtcat tcgcggcggc gcaggcattt actatgacaa
     2821 cacaattgac aatctacgac tcttcgagcg tgccgatctt ggtcctccag gagcgcagct
     2881 tttcctcgtc ggtttcgatg tgaacagccc gctgctgcca ggtgatggcg atggccgctt
     2941 cagcactaac cctgcatcgg cgagcggatt catcactctc agagacatgc tggcgctcgc
     3001 gccttcggtc cgagcagaca ttgaatcgcg agccttcaac tgcacacttc cgacttcgat
     3061 tgagtgtttt gacgccattt ccgggccgct cttctcaacc gagttccagg tgccttactc
     3121 gatccaatac tccgtcggct tccagcgcga acttcccggc aacatggtcg tgcaggcgga
     3181 ctacaattac cgtaagggcc tgcatgaggt gatcatctat gacgccaacc gcttgttcga
     3241 tttgacgatt ccaggtcaac aacggacggc cttcccgaac accattccct acgccgactc
     3301 ttcggctttc tcgacttacc aagcgctgct cgttcgcctg gatcgccgtt tctcaaaagg
     3361 cttccagatg acagccagct actcgctgtc gagattcaaa gccttcggcg gagactcgct
     3421 cggcctcggc gtagtcatca ccgatcagaa cgacttccgt aaggaattcg gacccggtgg
     3481 cctcgaccgc acacaccgac tcgtggtgag cggtctatgg gatctgccct tcttcaagaa
     3541 gagcgatagc tgggccaagc gcaatctgct cggcaactgg caggtttcga tgatctcaac
     3601 agctttcagc ggtctgccgc agcaagcttt ccttccacac ttcgcggatc tctcacgaac
     3661 cggcacgttt aactcttacc tgccgggaac aggaccggga tccctgggtc gtggcattag
     3721 aagtgtcaac gagttgaacg acatcattac ggcgtacaac aacagcatcc cgacactggg
     3781 cgaaccgtgc agtctgtcga ttagctcgac gggccgctgt gatagtaacg acccgtccta
     3841 ccgtgacccg ctcgagaggc tggccctgct gcccgccaac cagcctatcg gtggcgactc
     3901 gatcatctcg caggatctgc gagtgacgaa gacgttcgac atcagcgaga ccgtcaagct
     3961 cgacttgatc ggcgaggtgt tcaacctctt caacgtagcg aacctgactg gggttactga
     4021 cttcattctt ccggatgaag gtacgccccc agatcagatc acgttccttc gcccggcgca
     4081 gcgctccacc agcgttttcg gcactggtgg ccctcgcgcc ttccagttcg cggcgaaatt
     4141 ccgcttctag cgaattcgtc tcgacggttg acgcagtaca aaggtggggc ctgaaaaaca
     4201 tggccccacc ttttctttat tcaggtgtaa aaccgattgc acgatttggc attatgggag
     4261 ggtgattcga tgagcgcgca aatcaagaga ttcgtgtttg tttccatcat cgtgctgtcc
     4321 tgctgcgcct cgctcttggc ccaggggcat tcgatacgag ggaaagttag gaatgcggct
     4381 ggtgtgaacg tcggccgtag ccctgtaacg ctggaacgaa acggcgccat ggtggaacaa
     4441 accgtcacca ataatgaagg tgacttcact ttcagtggtc taacagacac gagttataca
     4501 gttatcgtaa cggcggttga ttacaacccc gcaagtgaat ccgtcgaatt tgtcagggcc
     4561 gctgcatcag accagcccgg agaattgcgc acggtagaaa ttaccctgac atccagggaa
     4621 cctgtgcgac cgccgagagc aggaataaat tttgtacaaa acattccggc tcccgccctg
     4681 gctgcttttg aagcgggact aaagcactca cgtgaaaacc gtccaacaga agctatcgcc
     4741 tcgtatcgag aggcaatcaa gatctttcca aattacttca acgccaattt gacgctcgcc
     4801 gctgaacttg ccaaacaagg tcagttgcag gaagcgatta cgagtttaga agctgcccgc
     4861 gccgcgaatc ctaaagacga tcgggtctac gacctgtttg cccgggtgat gatgcagcag
     4921 cacaaatact cggtggcggc tcgcatttac gccgaggccg cgcgactaaa tccggcggag
     4981 actcaatacc tcctcgcccg ggggacggcg ctggttgagc aggtagttgt tattgatggc
     5041 gcgccatcga ctgcggcagc ggatgaacgt tcatttgctc ttgctgaagc agagggagtc
     5101 ttgtcgcagg cggtaaagca gaacgagaaa ctcgcggatg ctcatttgca actggcgcgc
     5161 gtttacgaaa agaaggggga acgtgcgcga gcagcagagc aactagagcg ttacttgcgt
     5221 aaagcaccaa acgcgaagaa tgccgagcag atcaaggcgg cgatcaaaac cctgcgccaa
     5281 tagtcagttt gtcagtagac ggtagaaggt agaccgtaga cggtagtcag cgggcctcct
     5341 accttacagc tactgacaac ggatcaccga ctacgatcta ctgtctaccg tctacttctt
     5401 caaaaacttc gcaattccct gctggcaatc ctcgcccagt ctggcgatca cattcacatc
     5461 agcgcccgtc tcgatcgctt ctcgaaaagc catgccatcg atctgataaa gcaacgactt
     5521 tgtgagcgcg actgccgacc gactcatctc tgcaaacaca ccaacatacg cctggacctc
     5581 agagtcgaat gtttcatccg caaacacctg gttaactaaa ccgacctcgt aggccctgcg
     5641 ggcggtgatt tcgccgccgc gcgtaattag ttcgaacgcc tgcttttctg aaacgttgcg
     5701 cttcagtatc gccatcacca tcgccgggac gaacccaatc tttacctccg gataaccaaa
     5761 ccgcgcagac tccgcagcga gcaccagatc agcggcgctt gccagcccgc aaccacctgc
     5821 caaggcccgg cctcgcaccg ccgcaatcac agggatttcc agctctcgca tcaacgtgaa
     5881 taggtccatc attgagcgag cgtcggcagc gttctctgca atcgacgcct ccgagatctt
     5941 tcgtagtgct gacaggtccg cgccggagca aaaatctttt ccggcaccag agataaccac
     6001 tgcccgaacc gacggctcgg cggcagcggc ccgcaggccc ttcttaaggc cggcaatcaa
     6061 ttcatcgttg agggcgttgc gcttgtcggg acggttaagc ttaatagtcg caatagagcc
     6121 ggcgacggaa taaatggtcg cgctcatctg gttttcttca ggcattcatc gcctccgatg
     6181 gactttggca gaactttcaa gcagagcaac atcagacctt agctgccagc ggctgtcaaa
     6241 ttatgtcacc tttgctcttc attttttccc gcgcagcacc ggtgagggat gacataatga
     6301 gttcgccgcg cggtcggcat tcatgctcgg tctttccacg attatctgtt tatcccagct
     6361 cctttgcagc gctcagaaac caacgcccta ctcagtagac cgactcgccc ccgagattct
     6421 tcactcctgt cagccggaac ctgaccactt gaaaaccatg actgctaacc caaagccttt
     6481 cacgcaagcc aaattgatcg agctcatcag ccgtgtcgtg ccgtcgacat tctttctgtt
     6541 tgtcgtaatg ctgaaaatcg cggaactgta tgcctcgatc atcactcatc aagccacgaa
     6601 ccaaccaact tcagcgaaat tcatagttga agtctgctcg cgagcttcga cggcggcatt
     6661 cctcggactg atgaccatac tgtttttgat caggctggaa cctgttgaga aggccaagag
     6721 tcttttgccg cgtgttatgg ctatcgctgg taccttcttc gttgccttgg tgactttgtt
     6781 cccgcgtgcc gagctttcac tcacccaaag tctggtcgcc acctcgatta gcatcgtggg
     6841 aacaatcctt tcaatcatcg ctctcgcaca cctcggacga tcgttcagcc ttatggccga
     6901 agcaagacgg cttgtcacca ctggccctta ccgtgtcgtc cgtcatcctt tgtatctatt
     6961 tgaagaagtg gcatcgttcg gaatcctgct acaattcttg tcggttacca ctgctttgat
     7021 tttcgtgatc catattctga ttcaggtgca gcggatgaag aatgaagaga aggtgttaga
     7081 aaaagccttt cccgagtacc aggcctataa ggcgaccact gcacgcgtga ttcccaagct
     7141 ttattagttc tccaccgggg caacccttaa tctcaggatc gaagcagcgg aaccaagctc
     7201 gagataaccg gttaggtata tctccggcgg cgtaaaagaa tgcctcaagc tcacaaaata
     7261 aattggaaat ggggagtact tgctgcgctg gtaatcaccc tgcttgcact cattcctcag
     7321 ttctttatgt ggggcactcg cggttcgcaa tggaatggca gttatgccga acttcatggc
     7381 gacgaatggg tttactcagc ttacgtgcaa gcgctgatcg acggtcgtcc gcgtcgcagt
     7441 gatgcctaca ccggacgtga cgaacgaccc ggccatccgg aaccggagtc cctcttctcg
     7501 attcagttcg tgcccgccta cttgatagcc atgccagcgc gagctctggg gatatcatca
     7561 tccacagcat tcctcatctt aggactcctg gcgccctgtc tctcctgcct ggcgatcttc
     7621 tggttgttcg tcaacctgac acaagatgag aagctagcga gtgccgcagc gctcgtcgtg
     7681 ctttccttcg gtgccctcgc agccggtgag ggtctggttc atctctttat ctcgggatca
     7741 aactactctt tccttccgtt cctgcgcagg tatgaaccgg ttgcgccctt tccgcttttc
     7801 tttgttttct gcacgttaac ctggaaatct ctcaacgacc gcaacaagtc cctggtctgg
     7861 gcggggattg ctggtgtttg tctggcgcta ctgatctttt cctattactt tctttggact
     7921 ccggccctcg cctggctgat ctgtctggcg tttctctggc ttatcagcaa ccgtgccgag
     7981 ttgcgacgag ggattccagt atttgcaatc atcctgctga gcgccatagc tgcactgatt
     8041 ccttatttca tcctggtctc taatcgttca cacacaatgg atgccggcca gaagttggtg
     8101 ctctctcatg cgcccgactt gtttcgcgtc ccggagttgc tcggcctcgc ggtatttacg
     8161 ctgattgcca tcgcagcaaa aacaggccgc gtcgactggc gggctccagc gaccttattc
     8221 gctgcctctt tcgcactgac accgctgctt gtgtttaatc aacaaataat tacgggccgt
     8281 tcgctgcaac cctttcatta tgagtggttc atcgcaaatt atctggcatt gtccgggctg
     8341 gtagtgatag cgattctact ctggcgcggg cgggatcagg cgagaccgat tcggtaccgc
     8401 gtagccggaa ggattgtctt cgttgcgctc tggtgggcag cgatcgaagt acttgccccg
     8461 ggaaaggtga tcatccggga cagtcagttt acggatcgag cggccgcagt gtgtcaacga
     8521 ttaagagaat tatctgcgag cgagagagga accacctcag aactcgatcc actcccgatc
     8581 gttctcgcca gcgaaaacaa agtagccgtg atccttccga cattcacttc gcatgcggtg
     8641 ttttgggcgc cgcatttcga tttcctgcac ctcgctcccg gtgagagtcg cgcgcgcttc
     8701 tatcagtatc tctactacac gggcgttg
//
LOCUS       DASUEX010000663         4528 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_1715441, whole genome shotgun sequence.
ACCESSION   DASUEX010000663 DASUEX010000000
VERSION     DASUEX010000663.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4528
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1715441"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1141
                     /locus_tag="VFV61_11035"
     CDS             <1..1141
                     /locus_tag="VFV61_11035"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX4901057.1"
                     /translation="NDKLTFSSVKNVQLLQPLFEFSGACSGCGETPYLKLLTQLFGDR
                     AIIANATGCSSIYGGNLPTTPYTINREGRGPAWSNSLFEDNAEFGLGMRLALDHQESY
                     ARHLIEELRGSVGDNLAESLLKTDQSTESGIASQRELVAALKARLKDEKSNQARDLIN
                     VADSLVNRSVWIVGGDGWAYDIGYGGLDHVLASGQDVNILVLDTEVYSNTGGQASKAT
                     PLGAVAKFASGGKPSAKKDLGLTAMRYGNVYVAQVAMGANDTHTVKAFLEAEAHPGPS
                     LIIAYSQCIAHGIDMAKGMQQQKLAVDSGHWPLFRFDPAREIANANPFQLDSHEPKVP
                     LQDYIYTEGRYRMLQQSDPETAKLLLEQAQKVVNHRWQQYKKLAT"
     gene            1151..2149
                     /locus_tag="VFV61_11040"
     CDS             1151..2149
                     /locus_tag="VFV61_11040"
                     /inference="COORDINATES: protein motif:HMM:NF005741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HEX4901058.1"
                     /translation="MSLTTNYLGLALKNPIVVSACPLSHNIDSIRRLEDAGASAVVMY
                     SLFEEQITFDSFYIDYYLQKGTNSHAESLTYFPEMDQYNVWPDEYMNLIRRAKEAVDI
                     PIIGSLNGVSTGGWTDYAGLIEDAGADALELNVYYVPTSVELSGHEVECIYLDLLSQV
                     KASVSLPIAMKLSPFFSSTANMAKRLVEKGADGLVLFNRFYQPDLDLESLSVTPRLVL
                     SNSNELRLPLRWVAILYGRLNADLAITSGVHTAEDVLKGLMAGAKVTMMASELLQNGV
                     RRIKEILKDMEQWMDEREYASVSQMIGSISQQHCAEPAAFERANYMKTLDSYRPIN"
     gene            2166..3266
                     /locus_tag="VFV61_11045"
     CDS             2166..3266
                     /locus_tag="VFV61_11045"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HEX4901059.1"
                     /translation="MPRTVPIVNQPPVPQLRDASTKVPTRVRHLKHMIGNTPLLAVRL
                     LFRGKLRVVYAKAEHINMTGSIKDRMAFHILKQAYLEGHIKVGDTIAEATSGNTGISF
                     AAIGRALGHPVVIFMPDWMSQERVALIKSLGAQVVPVRHDQGGFLGSIRMTETLARET
                     ADVFLPSQFANEANVEAHEKTTGPELWWQLFFNSITPDAFVAGVGTGGTIMGVGSFLK
                     QRNPKIRVHPLQPAESPTLSTGHKVGKHRIQGISDEFIPSILHLDELDSVVSVHDGDA
                     ILMAQKLAASLGLAVGISSGANFLGALMVQDEMGGDAVVATVFPDDNKKYLSTDLLRD
                     EPVKEGYLSSEVELLSYQAFKRVCHTCLDDSD"
     gene            3298..>4528
                     /locus_tag="VFV61_11050"
     CDS             3298..>4528
                     /locus_tag="VFV61_11050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4901060.1"
                     /translation="MREKLIAEYHRMLTMDESLTGEFIASLKGMMRTRQLLYGEREIG
                     VALRPHLLTRTQFNLLTTSSEAVAEACNKVAAVLLAEPWRMEGIGLTEREVQLALVEP
                     KYAAPAVTSRLDAFVYGDEIKFVEYNAENPSSLSDPEGLNDVLLEVGAMRTIAERYQL
                     SQFSPALTLLDALLDTFREWGGVGAPNIAILDWEGLPTAREFVLLQEVFMSRGVQAVI
                     CTPGELEYDNGKLHCGAFPIDLVYKRVVIHEFLARCDNSHPLIQAYMHGDVCLVNNFR
                     CKMLHKKATFELLTDAASASWFTPHEREVIARTVPWTRRVAERKTQHGGQEIDLVPHI
                     RKNRQRFVLKPNDDYGGRGISIGAHTTAVEWDAALSEALAGDYVVQEKIELQTEVFPI
                     FGESGWDFQPMYVDTNPF"
BASE COUNT         1065 a         1242 c         1250 g          971 t
ORIGIN      
        1 caacgacaag cttaccttta gttcggttaa gaacgtgcaa ttactacaac cgctgtttga
       61 gttttccggt gcttgctcgg gctgcggcga aacgccttat ctcaagttgc tcactcagct
      121 cttcggtgat cgcgcaatca ttgcgaatgc caccggctgc tcgagtatct acggcggcaa
      181 cttacccacg actccctata cgatcaatcg cgaaggtcgc ggtccggcat ggagcaattc
      241 actctttgaa gacaatgccg agtttgggct cggcatgcgt ctcgccctcg atcatcaaga
      301 gtcttacgcg agacatttaa tcgaggagtt gcgagggtcc gtcggcgaca acctcgccga
      361 aagccttctc aaaaccgatc agtcaactga atccgggatt gcctcgcaac gcgaactggt
      421 cgctgctttg aaggcaagat tgaaagacga gaagagcaat caggcgcgcg atctgatcaa
      481 tgttgctgac tcgttagtga accggagcgt ttggattgtt ggcggcgatg gctgggccta
      541 tgacattggc tatggcggcc tggaccacgt gctggcgagc ggccaggacg taaacattct
      601 ggtgctcgac accgaggtct attccaacac cggcgggcag gcatcgaaag cgacgcccct
      661 cggcgcagta gcgaaattcg cctcgggcgg gaagccgagt gcgaagaagg atctcggact
      721 gactgcgatg cgctacggca acgtctacgt ggcacaagtc gcgatggggg ctaacgacac
      781 tcatacggtg aaggcgtttc tggaagcgga ggctcatccg gggccgtcgc tgatcattgc
      841 ttacagtcag tgcatcgccc acggcatcga catggccaaa ggaatgcagc aacagaagct
      901 ggcggttgac agtggtcact ggccactctt tcgctttgat ccagcgcggg agattgcaaa
      961 cgcgaacccg tttcaacttg attcgcacga gccgaaggtt ccgttacaag actacatcta
     1021 caccgaagga cgttatcgaa tgttgcagca gagcgatccc gaaaccgcga agctgctgct
     1081 ggaacaagcg cagaaagtcg tcaaccaccg ctggcagcaa tataagaagt tggcaacgtg
     1141 aggtaaacga atgtccctaa ctacgaacta tcttggtctg gcgttgaaga atccgatcgt
     1201 agtctcagcc tgccctctgt cgcacaacat cgatagcatt cgtcgtctcg aagatgccgg
     1261 cgcaagtgcg gtggtgatgt actcgctttt tgaagagcaa atcactttcg acagtttcta
     1321 catcgactac tacttgcaaa agggcacgaa tagccatgca gagtcgctca cgtactttcc
     1381 ggaaatggat cagtacaacg tctggcccga tgaatacatg aacctaattc ggcgcgcaaa
     1441 ggaggctgtg gacattccca tcatcggcag cctcaacgga gtttccaccg gcgggtggac
     1501 cgactatgca ggtctaatcg aagacgccgg cgctgacgcc ttggagctta atgtctatta
     1561 cgtccccacc agcgtcgaac tcagtggcca tgaagtagag tgcatctacc tcgacctcct
     1621 aagtcaggta aaggcctcgg tgtcgcttcc gattgcgatg aagctaagtc ccttcttcag
     1681 ttccacagca aacatggcca agcgcctcgt tgaaaagggc gccgatggac tcgttttgtt
     1741 taatcgtttc tatcaaccgg atctcgacct ggaaagcctt tcagtgacgc cgcggttggt
     1801 gctgagcaac tcaaatgagt tgcgcctgcc gttgcgctgg gtcgccatat tgtacggacg
     1861 cctaaatgcc gatctggcaa tcacgagcgg ggtgcatact gcggaggatg tgctcaaagg
     1921 cctgatggcc ggagcgaagg tcacgatgat ggcttcggaa ttactgcaaa acggcgtccg
     1981 ccggattaaa gaaatactca aagacatgga gcagtggatg gacgagcgcg agtacgcatc
     2041 cgtatcgcag atgatcggca gcatcagtca acaacactgc gcggaacccg cggctttcga
     2101 acgcgctaac tacatgaaga ccctggattc ttatcgaccg atcaattgat ccaacactta
     2161 tgcaaatgcc acgcaccgtt ccaatcgtca atcagccgcc tgttcctcag cttcgcgacg
     2221 ccagtaccaa agtgccaact cgcgtgaggc acttgaagca catgatcggc aacactccgc
     2281 tgctcgccgt gcgactactt tttagaggaa agctacgcgt cgtctatgcc aaggctgaac
     2341 acatcaacat gactggcagc atcaaagacc gcatggcatt ccacattctc aagcaggctt
     2401 atcttgaggg tcatatcaaa gtgggcgata cgatcgcgga agcaaccagc ggtaacaccg
     2461 gcatttcatt cgctgcgatt ggccgggcgc tgggtcatcc ggttgtcata ttcatgcctg
     2521 actggatgag tcaggaacgc gtcgctttga tcaagagctt aggtgcgcag gtcgttccgg
     2581 ttcggcatga tcaggggggc tttcttggca gcattcgcat gacagaaacg ctggcgcgag
     2641 aaaccgcaga cgtctttctg ccttctcaat ttgccaatga agctaacgtc gaagcgcatg
     2701 agaaaaccac tgggccggag ttgtggtggc agctattctt caattcgatc acgccggatg
     2761 cttttgttgc tggggtgggt acgggcggca ctatcatggg cgtgggaagt tttctcaaac
     2821 aacgcaatcc caagattcgc gtgcatcccc tgcaacccgc cgaatcgcca accttgtcca
     2881 cgggccacaa ggtcggaaag catcgcattc agggaatttc ggatgagttt atcccctcga
     2941 tcctgcacct cgacgaatta gactcggtcg tcagcgtgca tgatggcgac gccatcttaa
     3001 tggcgcagaa attggcggct agccttggtc tggcagtagg catttcctca ggcgcaaatt
     3061 ttcttggcgc tttgatggtc caggatgaga tgggcggcga tgctgtagtc gccacagtct
     3121 tcccggacga caacaagaag tatctaagca ccgatttgct gcgtgacgaa cctgtgaaag
     3181 aaggatatct ttcgtcggaa gttgagctac tcagctacca ggctttcaag cgagtctgcc
     3241 acacgtgttt agacgatagt gattagttgc ccccttcccg gttagacgcg gcgagaaatg
     3301 cgcgaaaagc tgatcgcaga gtatcaccgg atgctcacca tggacgaaag cctcaccggc
     3361 gagttcatcg cgagtttgaa ggggatgatg cgcacccgcc aactactcta cggcgagcgg
     3421 gaaattggcg ttgcgcttcg gccacacctg ctcacacgta cacagttcaa tctcctgacc
     3481 acctcctcag aagctgtagc agaggcttgc aacaaagtgg ccgcagtact cctcgcagaa
     3541 ccctggcgga tggaaggcat cggactcact gagcgcgagg tccaactagc gcttgttgaa
     3601 cctaaatatg ccgcccccgc cgtgacatcc aggctcgatg ctttcgtcta cggtgacgaa
     3661 atcaaattcg tcgagtacaa cgccgaaaat ccttccagtc tttcagaccc ggagggactc
     3721 aacgatgtgc tcctcgaagt tggtgccatg cgaaccatcg ccgagcgtta ccaactcagc
     3781 cagttctctc cggcactaac cctgcttgac gcgctgctcg ataccttccg cgaatggggc
     3841 ggcgtgggcg caccgaacat cgccattctt gactgggagg ggctaccaac tgcgcgcgag
     3901 ttcgttctgc tgcaggaagt attcatgagc cggggtgtgc aggctgttat ctgcactccg
     3961 ggcgaactcg aatatgacaa cggtaaattg cattgtggcg ccttcccgat agatctcgta
     4021 tacaagcgcg tagtcatcca tgagttcctg gctcgttgcg acaattccca cccgctaatt
     4081 caggcttaca tgcacggtga tgtctgcctc gtgaataact ttcgttgcaa gatgctgcac
     4141 aagaaggcga cgttcgaact gctcactgat gcagcgagcg cgagctggtt cactccgcat
     4201 gaacgagaag taattgcacg cacagtgccc tggacgagac gtgttgccga acgcaagacg
     4261 cagcacggcg ggcaggaaat agatctggtg ccacacatcc gcaaaaaccg gcaacggttt
     4321 gtcctgaagc cgaacgacga ttacggcggg cgcggcatct cgatcggcgc gcacacgaca
     4381 gcagtggagt gggacgcggc cctctcggaa gcacttgcgg gcgactatgt cgtgcaggaa
     4441 aagatcgagt tgcaaacgga agtctttccg atctttggcg aaagcgggtg ggacttccaa
     4501 ccaatgtatg tcgacaccaa cccgtttc
//
LOCUS       DASUEX010000664         3112 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_205802, whole genome shotgun sequence.
ACCESSION   DASUEX010000664 DASUEX010000000
VERSION     DASUEX010000664.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205802"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            110..2701
                     /gene="clpB"
                     /locus_tag="VFV61_11055"
     CDS             110..2701
                     /gene="clpB"
                     /locus_tag="VFV61_11055"
                     /inference="COORDINATES: protein motif:HMM:TIGR03346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEX4901061.1"
                     /translation="MRLDKFTLRAQEAIQSAIELAERNHNQQVEPEHLLAAMLEQPEG
                     IVRPLLGKLAVNHPVLLNDVQAAIGRFPRVEGGQQYLSPRLNQLFTAAQKQAEKAKDE
                     FVSTEHLLVAIAEEKDGEGGKILRQHGVKRDDLIKVIEQTRGGARITDQNAEDNYQAL
                     SKYAKDLTDLARKGKLDPVIGRDDEIRRTIQVLSRRTKNNPVLIGEPGVGKTAIVEGL
                     AQRIVSGDVPETLRNKRLVGLDLGSMLAGAKYRGEFEDRLKAVLKEIERAQGQIILFI
                     DELHTLVGAGAAEGAIDASNMLKPALARGELRCVGATTLNEYKKYIEKDAALERRFQQ
                     IYVGEPSVEDTIAILRGLKEKYEVHHGVRIKDSAIVAAATLSNRYITDRFLPDKAIDL
                     VDEAASRLRIEIDSLPQEIDKLERETIQLEIERQALQREEDAKSKARLKEIEQRIADL
                     KEASSGMKAKWQSEKEAIERMRKAKAELEQLKMQLDQARNAGDLGRASELQYGKIPEL
                     EQQLQAEQEKLAGLQSDGVMLKEEVDEEDIAEVVAKWTGIPVSKMLEGEMQKLVTMEE
                     RLSQRVIGQDDALNAVANAVRRARAGLQDPNRPVGSFIFLGPTGVGKTETARALAEFL
                     FDDERAMIRLDMSEYMEKHAVARMIGAPPGYVGYEEGGQLTEAVRRRPYSVILFDEIE
                     KAHQDVFNVLLQILDDGRLTDSKGRTVDFKNTVLIMTSNLGSREIQAAEGDEDEVRDA
                     VLEELRQHFKPEFLNRIDDIIVFHQLGQKEIAKIIDVQLERLRQMLQERNIVLVLDDS
                     ARQLLAREGYDPAYGARPLKRAIQTLIQNPLAVKLLNGEVLPGQTLNVSAAGDQMKFQ
                     TVMAAGA"
     gene            2722..>3112
                     /locus_tag="VFV61_11060"
     CDS             2722..>3112
                     /locus_tag="VFV61_11060"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX4901062.1"
                     /translation="MSKPWNPLHDLMLLQDRMNRLFEDATERRARATDSDEIETADWH
                     PAADVYDTVAAYLIAVDLPGVERSALDIDLDDEKLVIRGTRTLEKSGDKGKERVVRPH
                     GRFRRSFALPPNVASEGIQAEYRDGVLE"
BASE COUNT          796 a          788 c          908 g          620 t
ORIGIN      
        1 tcggtgacgt agaactactg gcaatccggc ggggccttat tcatgcggac agcatgatct
       61 taaatggccc cgctcagctt tcagagaaga aattctccaa cggagttaga tgagattaga
      121 caagtttact ttgcgggccc aggaagcgat tcagtcggca atcgaactgg ctgagcgcaa
      181 tcacaatcaa caggtcgagc ccgaacatct gctggctgcc atgctggaac aacccgaggg
      241 aattgttcgt cctctgctgg gcaaactcgc agtaaatcat cctgttttgc tcaacgatgt
      301 gcaggcagca attgggcggt tcccacgggt cgagggcggt caacagtatc tcagtccgcg
      361 cctgaatcaa ctgtttacag cggcacagaa acaggcggag aaagcgaagg acgaatttgt
      421 ctcgacggag catttgcttg tcgccattgc cgaagaaaaa gacggcgaag gcggaaagat
      481 cttgcgacaa cacggcgtca agcgcgacga tctgatcaag gtcatcgagc aaacccgcgg
      541 cggtgcgcgg atcaccgatc agaatgccga agacaactat caggcgcttt ccaagtacgc
      601 caaagacctg accgacctgg cgcgtaaagg aaaactcgac ccggtgattg gtcgggacga
      661 tgaaatccgc cgtacgattc aggtcctgtc tcgacgcacc aaaaataatc ccgtgctaat
      721 cggcgagcca ggcgttggca aaacggcaat cgtggagggc ctggcgcaac gaatagtttc
      781 cggagatgtt ccggaaacgc tacgtaacaa gaggctggta ggcctcgatc tgggatcgat
      841 gctcgccggt gccaagtatc gtggcgaatt tgaagatcgg ctgaaggccg tgctgaaaga
      901 gatcgagcgc gcgcaagggc agataatttt gtttatcgac gagttgcata cgctcgttgg
      961 tgccggcgct gccgaagggg ccattgatgc ttccaacatg ctcaagcccg ctttggcgcg
     1021 cggggaactg cgttgcgtcg gcgctaccac tcttaacgaa tacaagaaat acattgaaaa
     1081 ggacgccgca ctggaacggc gcttccaaca aatctatgtc ggcgagccta gcgtagaaga
     1141 tacgatcgcc atcctgcgtg gcttgaagga aaagtacgaa gttcatcatg gcgtgcgcat
     1201 taaggattca gccattgtgg ctgccgctac tttgtccaat cgctacatca ccgaccgctt
     1261 cttgcctgac aaagcgatcg acttggttga cgaagcagca tctcgtttgc ggatcgagat
     1321 cgattccctg ccccaggaga tcgataagct ggaacgggaa acgatccagc tggagattga
     1381 acggcaagca ttgcaacgcg aagaagacgc caaatcaaaa gcacgtttga aagagatcga
     1441 acagcggatc gcagatttga aagaagcttc gtcaggcatg aaagccaaat ggcaatctga
     1501 gaaggaagcg atcgagcgga tgcgcaaggc taaggccgag ctggaacagc ttaagatgca
     1561 gctcgatcag gcacgcaatg ccggcgacct gggtagagct tcggaactgc aatacggcaa
     1621 gattccggag cttgaacagc agcttcaggc tgaacaggaa aagctcgccg gcttacaaag
     1681 cgatggcgtc atgctcaagg aagaggttga cgaagaagac atcgcggaag tggttgccaa
     1741 gtggactgga attcccgtct ccaagatgct cgaaggcgag atgcaaaagc tggtgaccat
     1801 ggaagagcgt ttgagccagc gagtaattgg acaagatgat gctctgaatg cggtcgcgaa
     1861 cgctgtgcgt cgcgcgcggg cgggcttgca ggatcccaac cgtccggtcg ggtctttcat
     1921 tttcctgggt cccaccggcg tcggcaaaac ggaaacggcg cgcgccctgg ctgaatttct
     1981 ctttgacgat gagcgcgcga tgatccgttt ggacatgtct gagtatatgg agaagcacgc
     2041 ggttgcacgc atgattggtg ctccccccgg ctacgttggc tatgaggaag gaggccaact
     2101 taccgaagca gttcgccgcc ggccctactc agtgattctt ttcgatgaaa ttgagaaagc
     2161 gcatcaagac gtcttcaatg tactgctaca aattctcgac gatggccggc tgacggattc
     2221 caaaggtcgc actgtagact tcaaaaacac cgtgctgatc atgacttcca atctggggtc
     2281 gcgcgaaatc caggcagctg aaggcgacga agatgaagtc cgcgatgccg tgctggaaga
     2341 attgcggcaa catttcaaac ctgagttcct aaatcggatt gacgacatca tcgtattcca
     2401 tcagctcgga caaaaagaga tcgcgaagat catagatgtg caactggaac gtttgcgcca
     2461 gatgctgcag gagcgaaaca tcgtgttggt gctggacgat tctgcccggc agttactggc
     2521 ccgagaaggt tacgacccgg cttatggcgc gcggcccctg aagcgggcaa ttcaaacgct
     2581 gattcagaat ccccttgcgg tcaaacttct aaacggggaa gttttgccag gtcaaacgct
     2641 gaatgtttcc gccgctggag accagatgaa atttcaaaca gtaatggcag ccggcgccta
     2701 acggcccatt aggagagaag attgtcaaaa ccctggaacc ctttacacga tctgatgctc
     2761 ttgcaggatc gcatgaatcg gttgtttgaa gatgcaactg aacggcgcgc gcgtgccact
     2821 gacagcgatg agattgaaac tgctgattgg cacccggccg ccgacgttta tgacactgtg
     2881 gctgcatatc tcattgccgt cgacttgccg ggcgttgaac gctcggcgct cgacatagat
     2941 ctcgacgacg aaaagttagt cattcgcgga actcgcacgc ttgagaagag cggagataaa
     3001 gggaaggaac gagtggtgcg gccccatgga cgctttcgcc ggagtttcgc gttgcccccg
     3061 aacgtggcaa gcgagggtat tcaggccgaa taccgcgacg gcgtcctcga ga
//
LOCUS       DASUEX010000665         2413 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_675932, whole genome shotgun sequence.
ACCESSION   DASUEX010000665 DASUEX010000000
VERSION     DASUEX010000665.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_675932"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2178)
                     /locus_tag="VFV61_11065"
     CDS             complement(<1..2178)
                     /locus_tag="VFV61_11065"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX4901063.1"
                     /translation="MHGLEHYVGHVLDDKYRLERLLGRGGMGAVYLATHLGTARYVAL
                     KLITPQFMRNEEFVERFKREARAAGRLRHPNVVDVTDFGFAEVGGEPVAYLVMEYLDG
                     CTLGDVLAEEERLPLEWVVDILEQVCSAVHEAHEQGIIHRDLKPDNIWLEPNRLGGYR
                     VKVLDFGIAKLAELDAPAGEGSLPNQLRPLAGDLTGAGSSARLTQVPGNPNLEVDTLL
                     YSDGEQAALVADDNGSSALNDLANQEAQAEAARDTQFYSRPGVSTQAFAAPAPTIVPL
                     AADSGSTAAQSASAVKQQSEPEPEDRTLMLDQATRRVGSGPRTTLPAGTSQGAELTRV
                     GAIMGTPLYMSPEQCASKTLDARSDVYSLGVIAYQMLTGQTPFSGDMPSVMREHIENS
                     PPYLRSHNKKIKERVANVVMSALAKDPADRPQTAAAFASSLRAQAEGIGSLYRRALAL
                     YSEHFPKFFKLSVVAHIPVIVLTVALIGLELAEHAQPRGWGWVRILIVCAAALVGLAQ
                     VVAYFLAASAISGMTAIIVTQLSVAPLRPVELHSAFKVLKRRWKPFLKTAVGVTFRIV
                     IGIILLVIPGLVMSVRYALYAPVVLIEGLEKKAARMRARELASRSWKTVIIVSILQFV
                     IPTVFSVLVGRITVGSGAREGGVNVSGPSSISHQIYQQLIGLVNIVIIPLMSIVPALL
                     YLKMRQLGGERLVDAMAQIEEVDEERKEWQQRMRTKLSLATPRG"
BASE COUNT          544 a          692 c          663 g          514 t
ORIGIN      
        1 gcctcgtggt gtcgctaaac ttagtttagt acgcattcgc tgctgccatt ccttccgttc
       61 ctcatcgact tcttcaatct gcgccatcgc atcgaccaga cgctcgccac ctaactgccg
      121 catcttcaaa tacaacaagg ctggcacaat cgacattaac gggatgatga cgatattcac
      181 caagccgatc agttgttgat agatctggtg cgaaatcgat gaggggcccg agacgttgac
      241 cccaccctct cttgcgccac tgcctacagt gatgcgtcca accaacacac tgaacaccgt
      301 cgggatcaca aactgaagga tcgaaacaat aattacagtc ttccacgatc ttgatgccag
      361 ctcgcgcgcg cgcattcgag ccgctttctt ttccagtcct tcgattaaga cgacaggtgc
      421 atagagcgca tatcttactg acatgactag accagggatg accagcaaaa tgatgccgat
      481 cacaatgcgg aaggttacgc caactgccgt cttgaggaat ggtttccagc gccgcttcag
      541 gaccttgaac gctgaatgca gctcaaccgg ccgtaaagga gccaccgata actgagtgac
      601 gataatcgcg gtcatgcctg agatggccga cgctgcgaga aagtaagcaa ccacttgcgc
      661 caacccaaca agcgcagctg cgcaaactat caggatacgg acccaacccc aaccgcgagg
      721 ttgggcgtgc tcggccaact ccaaaccaat caatgcaaca gtgagaacga ttacggggat
      781 gtgcgcgacg acagacagtt tgaagaactt cggaaagtgt tcgctgtata gggccaatgc
      841 ccggcgatag agtgagccga ttccttctgc ctgggccctc agggagctgg caaacgccgc
      901 cgctgtttgc ggtcgatccg ctgggtcctt ggccagggcc gacatcacta cgttagcgac
      961 ccgctccttg atttttttat tgtgactgcg caggtaaggt ggcgagtttt caatatgttc
     1021 acgcatcacg ctcggcatgt cacccgagaa cggcgtttgt ccggtcagca tctggtaggc
     1081 aataacgccg aggctgtaga cgtccgagcg cgcgtccaat gtcttcgagg cacactgctc
     1141 cggcgacatg tatagcggcg tgcccatgat tgctcccacg cgggtcaact cggcgccctg
     1201 acttgtgcct gcgggcaagg tcgttcgcgg accggagccc acacggcgag tcgcctgatc
     1261 caacatcaga gtgcggtctt ccggttcggg ctctgattgt tgtttcactg cagaagcaga
     1321 ttgagcggca gtcgatccgg aatctgcagc taacggcacg attgtcggcg ccggagcggc
     1381 gaaagcttgg gtgcttactc ccgggcgcga gtaaaactgc gtatcgcgcg cggcctctgc
     1441 ttgagcttct tgattagcta gatcgttgag ggcgctggat ccattgtcat ctgcgaccag
     1501 ggccgcttgt tcgccatcgg aatagagtag cgtgtcgacc tccaaattcg gattgccagg
     1561 cacttgtgta aggcgtgcac ttgagcccgc gccagtgagg tcgccggcaa gtggtcgcag
     1621 ttgatttggc aggctgccct caccggccgg ggcgtcgagc tccgcaagtt tggcgatgcc
     1681 aaaatcgagt actttaacgc gatagccgcc cagccggttc ggttcaagcc agatgttgtc
     1741 aggtttcaga tcacggtgga tgatgccctg ctcatgagcc tcatgaaccg cagagcaaac
     1801 ctgttccaaa atatcgacaa cccactcgag aggcagtcgt tcctcttccg ccaacacgtc
     1861 gccgagtgtg caaccgtcga gatactccat cacgaggtag gccaccggct ccccgccgac
     1921 ttcagcgaaa ccaaagtcag ttacgtcgac aacgttcgga tgacgcaagc gaccggcggc
     1981 acgagcctcg cgcttaaagc gttcgacaaa ctcttcgttg cgcatgaact gaggtgtgat
     2041 gagtttgagc gcgacatagc gagcggtccc caggtgcgtg gccagataga cagcgcccat
     2101 cccgccgcgc ccgagcagtc gctcaagccg gtacttgtca tcgaggacat ggccgacgta
     2161 atgctcaagt ccgtgcataa gacagttatg ctacaacaga cacaatcgat tgccgatggc
     2221 cgatttcgga attgccgact ggccagtttg taaatacgtg atcagcgcca ggaattgttc
     2281 tcgcgtccag ggattaaaca agatttggca atctcaaaat tggctattgg caggcgcgaa
     2341 tcgacaatca cttaagccgt tccattctat agatggtctc ttttgccgga gccgttgcgg
     2401 tcgcaggcaa ggt
//
LOCUS       DASUEX010000666         3606 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_340686, whole genome shotgun sequence.
ACCESSION   DASUEX010000666 DASUEX010000000
VERSION     DASUEX010000666.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3606
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340686"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1925)
                     /locus_tag="VFV61_11070"
     CDS             complement(<1..1925)
                     /locus_tag="VFV61_11070"
                     /inference="COORDINATES: protein motif:HMM:NF019317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HEX4901064.1"
                     /translation="MGLRPRFETPLTRARDSVARYLVGPLKVLPLRGQSIVGVSLLVL
                     ITTLLLASGYSSTFAEDYKEGEIVRRTVVAPADINTIDIAETERRRNAARESTRPIFN
                     FDSTRSASSAQSFRAAWGDLQQQVENKNAESKTPVWHGEGGAPVARAIIAHKFDPVEL
                     DRLTGTLRNLADGYVYDDGDADRLKHEIVLVDVRNPAAQMIVPSPQTRMTPVTEVRRN
                     LELRLLELPGWSPDEKAALIAAMLPLIRPNVVLDETATASARETEANNIPQIPISLKR
                     NQVIAREGDTVTPAVLAQFVALRSTGRSGRPWHYLLGLLMVVGAVYWAAWKFTEHRST
                     TAALSLSARKAFALVGSAIVVETALMRVGFTVGDSVALQMKSVPLNDPTIWSFAIPFA
                     AAALLVAMLVDTQLAFLTGIVTALFAGILAPAGVPAIAFAMISCSAAVYGFGRYRERQ
                     SVTVAGLITGAVNILMALSLIAYAEQAFTLKTILLAIGCGFAGGLLTAIFTAGGLPIN
                     ESFFGILTDVKLLELSNADLPVLGQLALRAPGTNQHSHAVGQLAEDACRAVGANPLLA
                     RIGALYHDIGKLAAPEYFVENQQGDNPHDHLRPPQSARIITSHVTYGQRLAKEIGLPS
                     KIADFIPQHHGTRTLHF"
     gene            complement(1941..>3606)
                     /gene="hflX"
                     /locus_tag="VFV61_11075"
     CDS             complement(1941..>3606)
                     /gene="hflX"
                     /locus_tag="VFV61_11075"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEX4901065.1"
                     /translation="RRIPPHQVVTPEFARQIAELSHETRRQIGVLIDRKGHIEYVMVG
                     DNRRIELPDFKRIRVASGRFRGLRCIHTHLRGEELTQDDLTDLALLRLDLMAAIDVEP
                     ESGLPSLVRAAHLLPMHAGELQNVGGTAPYAFIEPKPPSQLDLDFLALITSLEDEMAR
                     NRQVTRRAETRDRAILVSVATGLTSDAEESMAELTELAASAGVVVQDQIIQRRTAVDP
                     RTVLGRGKLDELLIRALQLGADMLIFDQELAPAQVRSLSEATDLKVIDRAQLILDIFA
                     QRAQSREGKIQVELAQLKYLLPRLISGQDSAFSRLAGGIGGRGPGETKLETDRRRVRD
                     RINRLEKEIEIQKQRRQERRKERTRNKLPVISIVGYTNAGKSTLLNTLTARDVRAERR
                     MFATLDPTSRRLRLPREREVIINDTVGFIRDLPPDLLTAFRATLEEISESSLLIHLVD
                     LSNHRFEQQIESVERILRELKLAEIPTLVALNKSDIVERDAQQAAMRQMTQQGRDAVA
                     ISATNAASLRPLLEKAGTVLARDLTGQPLSESADTDPDAERLSLIA"
BASE COUNT          773 a         1030 c         1033 g          770 t
ORIGIN      
        1 aagaaatgaa gggtgcgagt cccgtggtgc tgcggaatga agtcagcgat cttggaaggc
       61 aggccaatct ccttggctaa tcgctgacca tatgtcacgt gactggtaat gatccgggca
      121 ctttgcggcg ggcgcaggtg atcgtgagga ttatcacctt gttgattctc gacgaaatat
      181 tcaggcgcag ccagctttcc aatgtcatgg taaagcgctc cgatgcgcgc cagcagcgga
      241 ttggcgccga ccgcgcgaca agcatcctcg gccaactggc ccactgcgtg tgaatgctgg
      301 ttagtgccag gcgctcgcag ggcaagctgt cctaacacag gcaagtcggc gtttgagagc
      361 tccagcagtt ttacgtcggt taagattccg aaaaacgact cattgatcgg caagccgccg
      421 gccgtgaaga tcgccgtcag cagcccgccg gcaaagccac agccgatcgc gagcagaatc
      481 gtctttaagg tgaaagcctg ttcggcataa gcgatcagcg acaaagccat caggatgttg
      541 acggcgccag tgatcaatcc cgcaaccgtt accgattgcc gctcgcgata gcgtccgaag
      601 ccatagaccg ccgcggaaca ggagatcatg gcgaacgcaa tggctggcac cccggcaggc
      661 gctagaattc ctgcaaacag cgccgtcaca attccggtaa gaaaggccag ttgagtgtcc
      721 accagcatcg cgaccaacaa cgcagctgct gcaaacggaa tggcgaagga ccagatcgtt
      781 gggtcgttca gcggtacgct tttcatttgc aacgccacgc tgtctccgac ggtgaaacca
      841 acgcgcatga gcgcggtctc cacgacaatg gctgacccaa ccagcgcaaa cgctttacgc
      901 gccgaaagcg aaagtgcagc cgtggtactg cggtgttctg taaacttcca ggccgcccaa
      961 taaacggccc cgacaaccat taacagaccc agcaagtaat gccagggtcg cccactgcgt
     1021 ccggtactgc gcagggcaac aaactgggcc agcacggcag gcgttactgt atcgccttca
     1081 cgtgcaatca cttgatttcg cttcagggag atggggatct gcgggatgtt gttggcttcg
     1141 gtctcgcgcg cgctggcagt cgcggtctcg tcgagcacaa cgtttggccg aatcaaggga
     1201 agcatcgcgg caataagcgc tgccttttcg tccggagacc agccgggtaa ctccaatagc
     1261 cgtaactcga ggttgcggcg aacttcagtc accggtgtca tccttgtctg cggtgaaggc
     1321 actatcatct gtgctgccgg gttgcgcacg tcgacgagca cgatttcatg tttcagccga
     1381 tccgcgtcgc catcgtcgta gacataacca tccgcgaggt tccgtagtgt ccccgtaagg
     1441 cgatccaatt cgacgggatc aaacttgtgc gcaatgatgg cgcgggcaac cggggcgccg
     1501 ccctcgccat gccagacggg tgttttgctt tccgcgtttt tgttttctac ctgttgttgc
     1561 agatcgcccc aggcggcacg aaagctttgc gctgagcttg cggaacgggt ggagtcaaaa
     1621 ttgaagatcg ggcgtgtgct ctcgcgggcg gcgttccgtc tgcgttcggt ctcagcgatg
     1681 tcgatggtat tgatgtctgc gggggccacc accgttcgtc tgacaatctc gccttccttg
     1741 tagtcttccg cgaaagtaga ggagtagcca ctggcgagta acaatgtggt gatgagaaca
     1801 aggagggaga caccgacgat cgactggccg cgcagcggca gtacctttaa cggtcccacg
     1861 agatagcgag ctactgaatc gcgcgcgcga gttagcggag tctcaaatct tggacgtagg
     1921 cccatcgtcg cagtttgtcg tcaggctatt agtgacagcc gctcggcgtc aggatcagta
     1981 tcagccgatt ctgaaagtgg ttggcctgta agatcgcgag ccaaaaccgt tcccgccttt
     2041 tccagcagcg gccgcaaact ggcggcgttt gtcgctgaaa tcgcgaccgc atctcgtccc
     2101 tgctgagtca tctgtcgcat cgctgcttgc tgagcgtcgc gttcgactat atcggattta
     2161 ttcagggcaa ccagtgttgg aatctcagcc agctttaact cacgcaatat tctttcgact
     2221 gactctatct gttgctcaaa ccggtgattc gaaagatcca caaggtggat caagaggctg
     2281 ctttcgctta tctcctcaag agtcgcacga aaggccgtta agaggtcggg aggcaaatcg
     2341 cgaatgaaac ctacggtatc gttgatgatc acctcccgct ctcgtgggag tcgcaaccgc
     2401 cgcgacgtcg ggtcgagcgt tgcaaacatt cgccgttcag cgcgcacgtc acgggcggtg
     2461 agagtgttga gcagcgtaga tttaccagca ttggtataac caacaataga gataactggc
     2521 agtttgtttc tggttctttc cttgcgccgt tcctgccgcc gctgtttctg aatttcgatc
     2581 tctttttcca gccggttgat tcgatcgcgc acgcggcggc gatcggtttc aagtttcgtt
     2641 tcgcccggtc ctcgtcctcc gatacctccc gcgagacgtg agaacgcgga atcctggccg
     2701 ctgatcaggc gtggcaagag atacttcaac tgcgctaatt cgacttggat cttgccttcc
     2761 cgagactgcg cgcgttgcgc aaagatgtcg agaatcaact gcgctcgatc aatgactttt
     2821 agatcagtgg cttcggaaag tgaccgaacc tgggcgggag ccagttcctg atcaaagatc
     2881 agcatgtcgg cgccgagttg tagagcccgg atcaacaatt cgtccaactt tccgcggccc
     2941 agcaccgtgc gcggatcaac ggcagtgcgc cgttggataa tctgatcctg caccaccacg
     3001 cccgcagaag cagcaagttc ggtgagctca gccatcgatt cttccgcgtc tgaggttagt
     3061 cccgtcgcca cgctcacgag gatcgcgcga tctcgagttt cagcacggcg ggtaacctga
     3121 cggttacgcg ccatttcatc ctcgagcgag gttatgagtg ccagaaaatc cagatcgagc
     3181 tgagaaggtg gcttcggctc aataaaagcg taaggcgccg tgccgcccac gttttgcaac
     3241 tccccagcgt gcatcggcag caggtgagcc gcccgcacca gactcggtag accactctcg
     3301 ggctcaacat cgatcgcggc cattagatcc agacgcagca aagccaagtc cgtcagatcg
     3361 tcttgcgtta actcctcgcc gcgtaagtgt gtgtgaatgc agcgcaagcc gcggaagcgt
     3421 ccgctggcaa cacgaatacg tttgaagtcc ggaagctcaa ttcgccggtt gtcgccaacc
     3481 atcacatact cgatgtgacc cttgcggtcg atgagaacgc caatttggcg ccgcgtctcg
     3541 tgggataact cagcgatctg cctggcaaac tcaggagtca ctacctggtg aggcggaatt
     3601 cgtcgc
//
LOCUS       DASUEX010000667         3049 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_206177, whole genome shotgun sequence.
ACCESSION   DASUEX010000667 DASUEX010000000
VERSION     DASUEX010000667.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3049
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206177"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>3049
                     /locus_tag="VFV61_11080"
     CDS             <1..>3049
                     /locus_tag="VFV61_11080"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX4901066.1"
                     /translation="LRQLAEGLHGLHETGKLHRDIKPSNVLVTKEGRVVILDFGLVAE
                     VAGKGLHESVNLAGTPDYMSPEQGAQLPISRASDWYSVGVMLYQALVGRLPFSGKFFE
                     VMMDKQNLDPATPAELVRDVPPELNELCVKLLKRAPERRPSGREILRVLGDGRTGPLR
                     APVLALTETPSRKRRAFVGRDRQTRELTNAFHATRQGHTVTCYVHGESGVGKSALVRN
                     FLDDLREHEGDTVILEGRCYERESVPYKALDGVIDSLTKFLMALPEAKAAALMPRDVL
                     ALARLFPVMLQVDAVFNAPQREQEIPDPFTLRRRAFAALRELLGRISDRQRLVIYIDD
                     LHWSDADSTTLLEDLLRPPDSPPLLLLASFRSEEIETKPFLEDLLQSAGTETRRELRV
                     TTLSDDEARILVQKLLSSASEPVVTSIAREARGNPFLLEQLARYAAASEQSATSGINL
                     SVMLDARLAQLPKGTRQFVDALAVAGRPINPEVAFHAAGLSGDELPLVSTLRAAQFLR
                     SGGSEHTVELYHDRIRETLAAQLDRSSVMQIHRRLAQAIETRGIDDPESLFEHYMGAG
                     ERMRAATHAAVAARKAAVALAFDRAAAFYRRALELAPGRGAEMIAMKRGLAEALVNAG
                     RPAEAAQAYLDLAQETSAAQALDSKRRAAEQLLIGGHIKEGLELVRSVLAAVGFTMPA
                     GPKRALLALLIKRIQIRMRGFNFTERKTSEIPEADLFRIDICWAVAAGLGAVDLIRGA
                     DFQCRHLLLALNAGEPFRIARGLAFEAAQSASRGVPAQARSLKIVQQAEALAQRVGHP
                     HALGLVTWARGITAYVNGRWKNAAELCEQAVEVLRDQCTGVTWELSIAHRFLFSAMVY
                     LGEIGEVSRRVPTLLGAALEQGNVFAATDLRTRLNLIWLAADDPDRARAEAIEALKAW
                     PHEGFHLQHYSSLLALAQIELYTGDADVAWKHILGQWPALENSMLLRNQTLRIEATYL
                     RARAELATALTSDKAAKLGFAETLASLFGSHAAWRLKQRKVLRV"
BASE COUNT          607 a          846 c          943 g          653 t
ORIGIN      
        1 ctttgcgcca actggcagaa ggccttcacg gtttgcacga aaccggaaag ttgcatcgcg
       61 atatcaaacc ttcaaatgtg ctggtcacga aggagggtag ggttgtaatt ctcgattttg
      121 ggttggtggc ggaagtggca ggtaaagggc ttcatgaaag cgtaaacctg gccggaacgc
      181 cggactacat gtcacccgag cagggagcgc aacttcccat ttcgcgggcc agtgattggt
      241 acagcgtggg cgtgatgctt tatcaggcac tcgtaggtcg cttgccgttt tccggaaagt
      301 tcttcgaagt gatgatggac aagcagaatc ttgatccggc aacacccgct gaactcgtgc
      361 gcgatgtgcc tccggagctc aatgagttgt gcgtcaaact cctaaagcga gcacctgagc
      421 gacgaccatc gggtcgtgag atcttacgtg tgcttggaga cggtcgcacc ggacccttgc
      481 gcgcaccggt gctggccctc acagaaactc cttcccgcaa aaggcgcgct tttgtcggtc
      541 gcgatcggca gacacgcgaa ctaacgaatg cctttcacgc gacgcgtcag gggcataccg
      601 tgacttgcta tgtacacggt gaatccgggg tcggcaaatc tgcgctcgtt cgcaacttcc
      661 tcgacgactt acgagagcat gagggtgaca cggtgattct cgaaggtcgt tgctatgagc
      721 gcgaatcggt tccgtataaa gcgctcgatg gtgtaatcga cagtctcacc aagtttttga
      781 tggccttgcc ggaagcaaag gcagcagcac tgatgccgcg agatgtgctc gcactcgcgc
      841 gtctctttcc ggtgatgctg caggtagacg cagtattcaa tgcgccacag cgcgaacagg
      901 agattcctga tccgttcacc ttgcgacggc gtgcatttgc ggctttgcgt gagctgctgg
      961 gtcgaatctc ggatcggcaa aggctggtga tctacatcga cgatctacat tggtccgatg
     1021 ccgacagtac gactttattg gaagacttat tacgtccgcc tgactcgccg ccgctcctcc
     1081 tgttggctag ttttcgtagc gaggaaatcg aaacgaagcc ttttctcgag gatctgctgc
     1141 agagtgcggg gaccgagacc cgtcgtgagt tgcgcgtgac gacgctgagc gacgacgagg
     1201 cgcgcatcct ggtgcagaag ctactgagtt cagcatctga gccggtagtg acttcgatcg
     1261 cgcgcgaggc gcggggcaat ccattcctcc tcgagcagtt ggctcgttat gcggcggcaa
     1321 gtgaacagtc tgcgactagc ggcattaatt tgagcgtgat gcttgatgcg cggctggcgc
     1381 agttgccgaa aggaacccga cagtttgttg atgcgctcgc agtagcgggc cggcctatta
     1441 atccagaggt ggcgtttcat gccgctgggc tctcgggcga tgaactgcct ctagttagca
     1501 cacttcgcgc cgcgcagttt ctgcgcagcg gcggttccga acacacggtt gagctctatc
     1561 acgatcgaat tcgcgaaacc ctcgcagcgc aacttgatcg ctccagtgtg atgcagattc
     1621 atcgccgcct ggcacaggcg attgaaactc gagggattga cgatccggaa tcgctctttg
     1681 agcactacat gggcgccgga gaacgaatgc gagcagcgac tcacgctgcg gttgccgcca
     1741 ggaaagcagc cgtcgcgctg gcctttgatc gcgccgcggc tttctaccgg cgagcgcttg
     1801 aacttgcacc ggggcgcgga gcggaaatga tcgccatgaa acggggactc gccgaggcgc
     1861 tggtaaatgc cggacgtcct gctgaagctg cgcaagccta cctcgacctg gcccaggaga
     1921 cttcggcggc ccaggcattg gattccaagc ggcgcgcagc agagcagttg ctgattggcg
     1981 gacacatcaa agaaggactc gaactggttc gctcggtgct agctgccgtg gggttcacca
     2041 tgccggccgg accgaaacgc gcgttattgg ccctgttgat aaaacgcatt cagatccgga
     2101 tgcgcgggtt taatttcact gaacgaaaga cgagcgaaat tcccgaagcg gatctgtttc
     2161 gcatcgacat ttgctgggcc gtggccgccg gcctgggcgc agttgatttg attcgcggcg
     2221 cagactttca gtgtcgtcac ttgctcctcg ctctaaacgc tggcgagcct tttcggatcg
     2281 cacgcggcct ggcgtttgaa gcagcgcaaa gtgcttcgcg tggtgttccc gcgcaggccc
     2341 gctcgctcaa gattgtgcag caagccgaag ctctggccca gcgcgtggga catccacacg
     2401 ccctcggcct cgtcacctgg gcacgcggaa tcacggccta tgtaaatgga cgttggaaaa
     2461 acgcggccga gctctgcgag caggctgtcg aagtgctgcg cgaccaatgc acgggcgtga
     2521 cgtgggagtt atcgattgcg caccggtttc tgtttagtgc gatggtctat ttgggtgaaa
     2581 tcggcgaagt gtcgcgccgc gtgccgacgc tcctgggcgc tgccctggag cagggtaatg
     2641 tgtttgccgc caccgatctg cgcacgcgtc tcaatttgat ctggcttgct gcagacgatc
     2701 ccgatcgcgc gcgcgccgaa gcaattgagg ccttgaaggc ctggccgcat gaagggtttc
     2761 acttgcagca ttacagctcg ctgctggcgc ttgcacaaat tgagctctat accggcgatg
     2821 ccgacgtggc gtggaaacat atactgggcc aatggccggc actcgagaat tcgatgctgt
     2881 tgcggaacca aacgcttcgt attgaagcca cttacttgcg cgctcgcgcc gagttggcga
     2941 ctgcattgac gagtgacaag gcagccaaac tcggtttcgc cgaaacgctg gcgagccttt
     3001 tcggatcgca cgcggcctgg cgtttgaagc agcgcaaagt gcttcgcgt
//
LOCUS       DASUEX010000668         2746 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_206357, whole genome shotgun sequence.
ACCESSION   DASUEX010000668 DASUEX010000000
VERSION     DASUEX010000668.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206357"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1209)
                     /locus_tag="VFV61_11085"
     CDS             complement(<1..1209)
                     /locus_tag="VFV61_11085"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HEX4901067.1"
                     /translation="MTQKRDGFAPATGDMEASEFRRFGHELIDWISDYFERIEELPVL
                     SQIEPGDLKAQLPTSPPAQGESMAEIIADVDRLIVPALTNWSHPSFFAYFATSTSAPG
                     IFGELLSAAFDNKAMLWRTSPASTELEEVTLDWLRQMMGLDKGYTGIIYDTASVSSMH
                     AIAAAREGVELRIREEGMSGRTDLPLLRVYVSEQSHSSIEKGVIALGLGQRSLCKIPT
                     DAEFRMDVAELKRAIEEDKAKGHVPFCIVATVGTTSTSSIDPVPDIIPIGEAHAMWLH
                     VDAAYAGSAAVVPELRNILAGCERADSVAVNPHKWLFTPFDLSVLYCRHMDLLRRAFA
                     LVPEYLKTPEQEKVRSGSDYGIQLGRRFRALKLWMIIRYFGHEGLAARIREHCRLAKL
                     FASWVEAADEW"
     gene            1661..>2746
                     /locus_tag="VFV61_11090"
     CDS             1661..>2746
                     /locus_tag="VFV61_11090"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX4901068.1"
                     /translation="MRRSLISLTIIAALLAAIACQPSGGGGDKVRIGVFMSLTGDTAN
                     FGISSTNGIKMAADEVNATGGINGKQIELLVQDDRSDASEAATIVTKFVTQDQVHAIL
                     GEVASSRSIAAAPIAQNAKIPMLTPSSTNPEVTKKGDYIFRSCFIDPVQGAAIAQFGA
                     RTLNAKRGALMVDRKNDYSTGLEKVINEVFTRLGGQIVVTQSYQAADQDFNAQITSIK
                     GANPDVIFVPGYYGDVGLFAKQARDKGITVPIVGGDGWDSPSLYQIGGAALNGCYFSN
                     HYSPDDADPLVQKFVNDYKSRYGSVPDALAATAYDAARIMFDAIKRANSLDGTAIRNA
                     LAATKEFPGVTGKVTFNENRDAVKPIVM"
BASE COUNT          655 a          787 c          713 g          591 t
ORIGIN      
        1 ccactcatcc gctgcttcta cccaggaagc aaacaacttt gccaggcgac agtgttcgcg
       61 aattctcgcc gccaggcctt catgtccaaa gtaacgaatg atcatccata gcttcagagc
      121 ccgaaagcgt cggcccaact ggatcccata gtccgaacca ctgcgcacct tttcttgctc
      181 gggcgtcttg agatactccg gcaccagcgc aaacgcgcgg cgcagtagat ccatgtgacg
      241 gcaatacaga actgacaaat caaaaggcgt gaacagccac ttgtgcggat tcaccgcaac
      301 tgaatcagcg cgttcacagc ccgccagtat gttccgcaac tcgggcacga ctgcggcaga
      361 accagcgtaa gccgcgtcga cgtgcaacca catcgcatgt gcttcgccaa tgggaatgat
      421 gtccggcacc ggatcaatgc ttgaagtcga agtcgttccc acagtcgcaa ctatacaaaa
      481 gggcacatgg ccttttgcct tgtcttcctc aatcgcgcgt tttagttctg caacatccat
      541 gcggaattca gcgtctgtgg gaatcttgca cagactccgc tgtcccaggc ccagcgcaat
      601 gacacctttt tcaattgatg aatgcgactg ttccgaaacg taaactcgca gaagtggcag
      661 atctgtgcgg ccgctcatgc cttcttcacg aatgcgcagc tcaacacctt cgcgggcggc
      721 ggctatggcg tgcatgctcg agacggaagc cgtgtcgtaa atgattccgg tataaccctt
      781 gtcgagaccc atcatctgac gcaaccagtc gagcgttact tcttcaagct cggttgaagc
      841 cggcgaggtg cgccagagca ttgccttgtt gtcaaaggcc gcgctcagca actcgccaaa
      901 gatacctggc gctgaggtag acgttgcaaa gtaggcgaag aaagaagggt gactccaatt
      961 cgtcagggcc ggcacaatca ggcggtcaac atcggcgatg atctccgcca ttgactcgcc
     1021 ctgcgctggt ggtgaggtgg gaagctgggc ctttagatca cccggctcaa tctgagaaag
     1081 aaccggcaac tcttcaatac gttcgaagta atcgctaatc caatcgatca actcgtgacc
     1141 aaagcggcga aactcagacg cctccatatc accggttgcg ggtgcgaacc catctctctt
     1201 ctgagtcatg gcgggcagtc taaattaggc aggagcataa ttccatcgtt cgtctcgcga
     1261 gggacgccag ggagagcacg ctcaattacg gtaagggagc ggtttcgacc gcattggtca
     1321 gacacgcgaa actctaaatc tctcattgat tctatgagca cggtcgcccg taccctagtg
     1381 aagcattggc gcttatctcg acacttatcg cgaaatcgct ataatcctcg ggccttcgag
     1441 acagaaaggg aatccgggca gcttcctttc ggatgcaggc tgccggaaag tttccccaag
     1501 tcgaattgcg tttgcgttac caactacggc gcggctatta ttcagcgcgt taattctgtc
     1561 taccagcaac gaggctcgaa tttgttcaaa ccgccccccg gtttcgaagc aactcgggct
     1621 caaactgtaa ccactaaaac ttactttctg tgaggaccct atgagacgca gtctaattag
     1681 cttaacaatt atcgccgctc tgcttgccgc aatcgcttgc caaccttcag gaggaggcgg
     1741 cgacaaagtt cgaattggcg tctttatgtc gctcacggga gacacggcca attttggcat
     1801 ctcgtcaacg aacggaatca agatggctgc tgacgaagtg aatgccaccg gtggcatcaa
     1861 cggcaaacag attgaattgc tggtgcagga cgatcgctcc gacgcttcgg aagcggcaac
     1921 gatcgtcacg aagtttgtga cccaggatca ggtacacgcg attctcggcg aagttgccag
     1981 ctcacgctcc atcgcggcgg cgccaattgc gcagaacgct aagattccaa tgctcacgcc
     2041 ttcctcaacg aatccggagg taacaaagaa gggtgattac attttccgca gttgcttcat
     2101 tgatccagtt cagggtgcag cgatcgcaca gtttggcgcg aggactctta acgctaagcg
     2161 cggcgcactg atggtcgacc gcaagaacga ctattccaca ggtctggaga aagttattaa
     2221 cgaggtcttt acgcgcctcg gcggccagat tgtggtgacg cagtcttatc aggcagcaga
     2281 tcaggacttc aacgcccaga tcacttctat aaaaggcgcc aacccggacg tcatttttgt
     2341 acctggctat tacggcgatg tcggcttgtt tgcgaagcaa gctcgcgaca aaggcatcac
     2401 ggtgccgatc gtcggtggtg atggctggga ctcgccgagt ctttatcaga ttggtggcgc
     2461 tgcgctcaat ggctgctact tcagcaacca ctactcacct gacgacgccg accccctcgt
     2521 gcagaaattc gttaacgact acaagtcacg ttatggttca gtgccagatg ctctggccgc
     2581 caccgcatac gacgcggccc gcatcatgtt tgacgctatc aagcgcgcca actcgcttga
     2641 tggcacagcg attcgcaacg ccctggccgc aactaaggaa tttcccggcg tcaccggaaa
     2701 ggtgaccttc aacgaaaacc gcgacgccgt gaaaccgatc gtcatg
//
LOCUS       DASUEX010000669         2841 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_678054, whole genome shotgun sequence.
ACCESSION   DASUEX010000669 DASUEX010000000
VERSION     DASUEX010000669.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2841
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_678054"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /locus_tag="VFV61_11095"
     CDS             complement(<1..343)
                     /locus_tag="VFV61_11095"
                     /inference="COORDINATES: protein motif:HMM:NF012663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4901069.1"
                     /translation="MTRRLKTIPNSRRLLGHSKHLSFLAFAILITSLWVPAAKTSSNT
                     EKPYDLIITNARIVDGSGNPWFRADIAILNGRITRIGRLSPAEAVKTIDAANQIVAPG
                     FIDVHTHVESIY"
     gene            complement(645..2381)
                     /gene="pabB"
                     /locus_tag="VFV61_11100"
     CDS             complement(645..2381)
                     /gene="pabB"
                     /locus_tag="VFV61_11100"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HEX4901070.1"
                     /translation="MIEATFGSRDLQAQGLPTHFQTPLKVLTAHRLDEVHPLLKAAAA
                     EAKAGHWVALLLSYEAAPAFDRAMTTRPARGLPLGWAAVFDAPASTPPQSIAEGYEVG
                     PWTPRVSRESFASAISQIRELIAAGDTYQVNYTFPLIAEFQGDARAWFRNLCRAQGAE
                     YCAYFDLGRYKILSVSPELFFELEGRTITTRPMKGTIGRGRWLAEDVQVAARLAASAK
                     DRAENVMIVDLLRNDLGRVAVAGSVNVKRLFELERYETLWQMTSTIEATLRGEVDFAE
                     LMARLFPCGSITGAPKIRTMEIIRDLEPFPRGVYTGTLGFLRPGGSAVFNVAIRTIVI
                     DSEDGLATFGVGGGITYDSTAEREYEECLVKSSFLNSKPVEFELLESMLLEAGRFFLL
                     ERHLERMKAAATYFGFCFPEMKIRLELEQIARRHAQGSCKTRLLVSRCGEVRTETQQR
                     EEVTPGAVRVALAARPVSANDKFLFHKTTNRAAYDDAMGSRPDCDDVILWNEAGEVTE
                     STVANVVVSFDGQLYTPPPEAGLLAGTLRAELIAAGKIDERVIRVADLPGASEIFLIN
                     SVSGWRQALLVP"
     gene            complement(2391..>2841)
                     /locus_tag="VFV61_11105"
     CDS             complement(2391..>2841)
                     /locus_tag="VFV61_11105"
                     /inference="COORDINATES: protein motif:HMM:NF045201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4901071.1"
                     /translation="IGLLETTNVSVGRGTNTPFEVVGAPWIKGQELALFLNRRNIPGV
                     RFVPLLFTPNASVFKDESCSGINIVITDRSKFRAVRTGIEIAVALRRLYPTDWKVDSY
                     LRLLVNAATLERVRRGDTPEEIERSWSASLAEFQRARARVLLYEEVR"
BASE COUNT          686 a          794 c          738 g          623 t
ORIGIN      
        1 tgtagatgga ctcaacgtgt gtatgcacat cgatgaaacc gggagcgacg atctgatttg
       61 cggcgtcaat cgttttgacg gcctcagccg gcgaaagtcg tccgatgcga gtaatgcgcc
      121 cattcaatat tgcgatgtcg gcacgaaacc acggattccc gctgccatca acgatgcgtg
      181 cattcgtgat tatcaggtcg tatggctttt ctgtattgga agatgtcttt gccgcaggca
      241 cccaaaggct ggtgattaag atcgcgaagg caaggaaact aagatgtttg gagtggccta
      301 aaagtcgacg cgaattgggg attgttttga gtctcctcgt cataagcaca gcaacgcgtg
      361 atttcacaga cttccgaatc cttgttggat actgaaaaca agatgatgct ctgcaaccga
      421 agtctccttc acgatgcgag ctaagtattt cgctttatta agaaacgagc gtaaagaact
      481 gacgaacgcc ctgattgatt gccgatcagg gcgcaagtat aaggttggtt ctggaataat
      541 ggtagctaaa gattcttatt caggcgataa atctctctga tagtgacgac gagttctcga
      601 atggcgcccc gacgctttgg agagacaagt gctcggctcc agcattaagg tacgagcaac
      661 gcctgccgcc atcctgaaac agagttgatt aagaagatct ctgaagctcc aggcaagtca
      721 gccacgcgga ttactctttc atcaatcttc cctgctgcta tcaattcagc gcggagcgtt
      781 cctgctaata acccggcctc aggcggcggc gtgtagagct ggccgtcaaa agaaacgacg
      841 acgttagcca cggtcgattc agttacctca cccgcttcgt tccaaagaat gacgtcgtcg
      901 cagtctggcc gcgagcccat cgcatcgtcg taggcagcac gattagtagt cttgtgaaac
      961 aggaatttgt cgttcgctga gactggcctg gcagccaaag ccacccgtac agcgccgggc
     1021 gtcacctctt cgcgctgctg agtttcagtg cgaacctcgc cgcaacgaga tacaagcaaa
     1081 cgagtcttac agctcccctg cgcatgcctg cgcgcaatct gctcaagctc caaacggatt
     1141 ttcatttcgg gaaaacaaaa gccgaagtaa gtagccgctg ccttcattcg ctccagatgt
     1201 cgctcaagaa ggaagaatct ccccgcttcg agcagcatcg attccaacaa ctcaaactca
     1261 actggcttgc tgttaaggaa tgaacttttg accaggcact cttcatattc acgttcggcc
     1321 gttgagtcgt aggttatgcc gccaccaact ccgaaagtgg caaggccatc ctccgagtcg
     1381 atcacaatgg tgcgaattgc cacattgaaa acagccgagc cgccggggcg caggaagccc
     1441 agcgtgccgg tatagacccc acgcggaaaa ggctccagat cgcgaatgat ctccatcgta
     1501 cgaatcttgg gagcaccggt aatcgaaccg cagggaaaca acctcgccat cagttccgca
     1561 aagtcgactt cccctctcaa ggtagcttcg attgtcgaag tcatttgcca cagcgtttca
     1621 taacgctcga gctcgaacag tcgtttgacg ttaactgaac cggcaacagc cacccggcca
     1681 agatcgttgc gcagcaaatc aacgatcatt acattctcgg cacggtcctt agcagacgcc
     1741 gccagtcgcg ctgccacctg cacatcttcg gctaaccatc tgccgcggcc gatggtgcct
     1801 ttcatcgggc gagtcgtgat cgtccttcct tcgagttcaa aaaagagttc tggagaaacg
     1861 gagaggattt tgtagcggcc cagatcaaag taggcgcaat actcggcgcc ctgagcacga
     1921 caaaggttgc gaaaccaggc acgcgcgtct ccttgaaatt cagcaatgag gggaaaagtg
     1981 taattgacct ggtaagtgtc tccggccgcg atcaactctc tgatctgaga aatggcactc
     2041 gcaaaactct cgcggctgac tcgcggagtc catggtccaa cttcataccc ttcggcaata
     2101 gactgcggcg gagttgacgc cggcgcatca aacaccgccg cccaacccag cggtaatcct
     2161 ctcgccggcc ttgtcgtcat ggcccgatca aaagcgggcg cggcttcgta gctcaagagc
     2221 agcgccaccc aatgaccggc ttttgcctca gccgctgcag ctttgagcag gggatggact
     2281 tcgtcgagac gatgagcggt cagtactttg agcggagtct gaaagtgtgt tggcaaccct
     2341 tgcgcttgca aatcacgcga tccgaatgtt gcctcgatca tgactggagt ttaacggacc
     2401 tcttcgtaga gcaaaacacg ggcgcgcgcg cgctgaaact cggcgaggct cgccgaccat
     2461 gaacgctcga tctcttcggg agtatcgccg cgtctgaccc gttcaagcgt cgcggcatta
     2521 acaagaagtc ttaggtacga atcgactttc cagtccgttg ggtagagtct ccgcagcgcc
     2581 accgcaatct caattccggt ccgcaccgca cgaaatttgg accgatcggt gatcacgata
     2641 ttgatgccgc tacaactctc gtccttaaat acagaggcgt ttggcgtgaa cagcagcggt
     2701 acgaatctga cgccgggaat gttacggcga tttaggaaca gcgcgagctc ctgacctttg
     2761 atccatggcg cgccaaccac ttcaaatggt gtgttagttc cccgccctac cgaaacgttt
     2821 gttgtctcaa gtaacccgat c
//
LOCUS       DASUEX010000670         3617 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_554978, whole genome shotgun sequence.
ACCESSION   DASUEX010000670 DASUEX010000000
VERSION     DASUEX010000670.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3617
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_554978"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(175..1797)
                     /gene="glpD"
                     /locus_tag="VFV61_11110"
     CDS             complement(175..1797)
                     /gene="glpD"
                     /locus_tag="VFV61_11110"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF008899.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HEX4901072.1"
                     /translation="MFASCLLVVRFAVGVRTLSDKGKGPAIALVDYDLIIIGAGINGA
                     GVARDAALRGLRVLLLDKGDVGGGTSSWSTRLIHGGLRYLEHGEFGLVRESLRERETL
                     LRIAPHLVRALPLLIPIYEGARRGRLLLRLGMSVYDLLSLGKTFPHHQMLSREQAIAR
                     APGLNTEGLRGAALYYDAQVEFAERLVLENVLSAVTHGARVVTYAGVDSFIVHNQSLI
                     GVEFVDVLNGERQSATAALIINAAGPWVDQVLKTTDAGAPLIGGTKGSHLIVPRFSGA
                     RETAIYVEARTDQRPFFVIPWNNNYLIGTTDIRFQGDLDNVRIDDDEIDYLLRETNGV
                     FPAAKLSRADILYSYSGIRPLPFTRDADEQSITRRHFIRAHPKFNNLLSLVGGKLTTY
                     RSLAEETTDLVFEKLGRTSPACLTAEEPLGALSNPIRTSPLTARLSRIYGGRSDAVRE
                     LVKEDASLGEVFDPETGGVAAEVVYAFRHEFARTLTDCLLRRTMVGLNSSLGVGADEA
                     AAKIAVKHLGWPEDRARAEVGSYRKFIERFRV"
     gene            2689..3606
                     /locus_tag="VFV61_11115"
     CDS             2689..3606
                     /locus_tag="VFV61_11115"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX4901073.1"
                     /translation="MKLILTIAFALTTTSPLNLAFQNQQEDFAKRSPPPVSAEAMSQM
                     EARLNEARARYEAEPNNPEAAIWFGRRLAYLGRFSEAIEVYSSGIKRFPRDARFFRHR
                     GHRYITLRKFDQAIADLKKAASLIKGRPDEVEPDGQPNARNIPTGTLQFNIWYHLGLA
                     QYLTGRNKDALDSYRECLKVSGNPDSLVATTHWRYMTLRRLQRTREAAQVLEPIRPGM
                     DIIENDGYYKLALMYKGLVDSETLLAETLKQPSSPGSLSILYGLANWHQYNGRGERSV
                     EVLQKIVSTDQWTSFGYIAAETDLKGLKT"
BASE COUNT          908 a         1010 c          889 g          810 t
ORIGIN      
        1 aaggcgtgaa ttcaatagcc atgggcaacg cccacggaac cgcatcgcta agaacattcg
       61 accctgaaag ggtcgaagcc ttctaaacac aggggagggc attcaaagtg ggcggcggca
      121 agccatcctt cctgactgag cattaactca tcgacgcact atcttgatca ccgcctacac
      181 tcgaaagcgc tcaatgaact tccggtatga accaacctca gcccgagctc tatcctctgg
      241 ccagcctaaa tgctttacgg caatcttcgc tgctgcctca tctgcaccaa caccaagcga
      301 tgaattgaga ccgaccattg tgcgccgcaa taaacaatct gtaagcgttc gtgcaaactc
      361 atgacgaaat gcataaacca cttctgcagc aacgcctcct gtttccggat cgaagacctc
      421 tcccaaggaa gcgtcttctt tgaccagctc tctcactgca tcactgcgcc ctccgtagat
      481 ccggctcaac cgcgccgtca acggcgatgt tctgattgga ttagacagag cgccgagcgg
      541 ttcttcagct gtcaaacaag ctggcgaagt tcgccctaac ttttcgaaga ccagatctgt
      601 cgtctcttcg gcaaggctgc ggtatgtcgt cagcttaccg ccgacgagcg acagcaagtt
      661 attgaatttc ggatgcgcgc gaatgaagtg tcgccgagta atgctttgct cgtcagcatc
      721 cctggtaaac ggtaacggcc taattccgga gtagctgtaa aggatgtcgg cgcgactaag
      781 cttggcagcc ggaaacacgc cattcgtttc gcgtaacaag tagtcgattt catcatcatc
      841 gatccgcacg ttgtcgagat cgccctggaa gcgaatgtct gtggtgccta tcaagtagtt
      901 attgttccag ggaatgacaa aaaagggacg ctgatcggtc ctggcttcca cataaattgc
      961 ggtctcacgc gcacccgaga atcgaggcac aatcagatgg ctgcctttcg tgccgccgat
     1021 caatggtgcg ccggcgtcag tggttttcaa aacctgatcc acccaaggcc cggcagcgtt
     1081 gattatcaaa gccgctgtcg cgctctgccg ctcgccgttc aatacgtcga caaactcaac
     1141 gccaatcagg ctttgattat gaactatgaa tgagtcgaca ccggcgtagg taacgacgcg
     1201 tgctccatgt gtcacggctg atagcacatt ctcaagcacc agccgttcag cgaactcaac
     1261 ctgagcgtcg tagtacaacg ccgctccgcg tagaccctca gtgttcagtc ccggtgcgcg
     1321 agcaattgcc tgttcgcgtg aaagcatttg atggtgggga aaagtctttc ccagggagag
     1381 cagatcgtag acgctcatgc caagtcgaag taacaagcga ccccgacgcg caccttcgta
     1441 aatcggaatc agcagcggca aagcacgaac gaggtgcgga gcgattctca gcagcgtctc
     1501 gcgctcacgc aaggattcgc gaaccagacc aaattcgcca tgctccagat agcgcaggcc
     1561 gccatggatc aatctcgtcg accaactgga tgtgccgcca cccacatcac ccttgtcgag
     1621 gagcagcact ctcaggccgc gcagcgccgc atcgcgcgct actcccgcgc cattgattcc
     1681 cgcgccgatg atgatgagat cgtagtcgac caaggctata gccgggcctt ttcctttatc
     1741 gcttaatgtc ctgacgccaa cagcaaagcg aaccaccaac agacacgaag caaacacgaa
     1801 agtgttcgtg tgattagtgg ttcgtttaat agtgtccgaa tctgaagttc agaatccgct
     1861 ctatggtact cgccttgtcc gttgtgattt ctccttcagc cgcaagagca agcccaaccc
     1921 gatcaggcca taaacggcga ccaatccaag gtactttaat gctctagtta caatgggcgg
     1981 gtcaaagcct ctcacgtact ttggcccact gagatagccc aacataacaa atagcatcag
     2041 gaacgatatg acgcaccaaa ctactagcag caacaaaccc ttgatcagat cacgagaggc
     2101 ttctccggct ctgacaaaca tcatgatagg aatcgctagc gcaatcccaa gcgctagcaa
     2161 gactggtagg atgaggatga tgtctttcat gctccgagat agtaccgctc actggaccta
     2221 atggataagc ttcgacaact acccacggtg ggcgttctcc ccttttgctc ttcaaattag
     2281 gacaatcaca tacttgactc accaagcaat gtcatcgtgt ttgggttcca aaagcgccga
     2341 aggcgcggca ttcaatagcc gcggccgtaa ggccgtggat ccaaggcctc aagatgaaat
     2401 cgaggcccgc agggccggca ttcctctgca cgatatgaga tttactgccg caccttcggc
     2461 gctcgattat ttaggttccg actgaccacg gcctcacggc cgtggctatt tgatgacggt
     2521 cctgcggacc tcacttggca atctcaggac gtttgttgat tccgctgaaa ttgagtcaag
     2581 tatgttatta cccaaattag gactacaggt tggttttgaa ttttaggatt tgactcgggc
     2641 tagctgaata tcatagaagt ctccgcccct tagaaagaga gtcgacaaat gaagctcatt
     2701 ctgactatcg cttttgccct aacgacaacc tcacctttga atcttgcatt tcaaaaccaa
     2761 caagaagact ttgccaaacg atctccgccg ccggtttctg ccgaagcaat gagccaaatg
     2821 gaagcgcggc tcaacgaggc gcgcgctcgt tatgaggcgg aacccaataa tcctgaagct
     2881 gctatctggt tcggtcgcag actcgcttat ctcggccgat tttctgaagc gatcgaagtc
     2941 tacagttctg ggatcaaaag gtttccgcgc gatgcacgat tctttcgtca tcgcggtcat
     3001 cgttacatca cgctgagaaa atttgatcag gccatcgccg atttgaaaaa agcagcgagt
     3061 ctcataaaag gacggcctga cgaagttgag cctgatggcc agcctaatgc gcgcaatatt
     3121 ccgaccggca ctttgcaatt caacatttgg tatcacctgg gtctggctca atatcttacc
     3181 gggcgtaaca aagacgcact cgatagttat cgcgagtgtc ttaaggtttc gggaaatcct
     3241 gacagcctcg tggctaccac tcattggcgc tacatgacgc tgcgccggtt gcagcgaaca
     3301 agggaagccg ctcaggtgct tgaaccaatc cgtcccggga tggacattat cgagaacgat
     3361 ggttattaca aactggcgct gatgtacaaa ggcctcgtcg attccgaaac attgctggcg
     3421 gaaacactca aacagcccag ctctcccggc tcactcagca ttctctatgg actcgcaaat
     3481 tggcatcagt acaacggacg cggtgaaagg tcagtcgagg ttctgcaaaa gatagtttcc
     3541 actgatcagt ggacgagctt tggatacatc gccgccgaga ctgatttgaa gggtttaaag
     3601 acatgaccgg tttttgc
//
LOCUS       DASUEX010000671         4576 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_555405, whole genome shotgun sequence.
ACCESSION   DASUEX010000671 DASUEX010000000
VERSION     DASUEX010000671.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4576
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_555405"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..618)
                     /locus_tag="VFV61_11120"
     CDS             complement(61..618)
                     /locus_tag="VFV61_11120"
                     /inference="COORDINATES: protein motif:HMM:NF016840.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX4901074.1"
                     /translation="MLTLFYDSHTMRPQPNEAASYYHGYINRVTEDDVVAVLERQLDE
                     TVAFLSEISEEHSLHRYAPEKWSMRELLNHVNDAERVFLFRALWFARGFAEDLPSYDQ
                     DVGVAGAQADEFDWANHIAEFRAIRLATLAFFRNLPAENLAESQNVWDRSGIASGNPF
                     TVRALAYIIAGHSAHHLAILKERYL"
     gene            1009..2652
                     /locus_tag="VFV61_11125"
     CDS             1009..2652
                     /locus_tag="VFV61_11125"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEX4901075.1"
                     /translation="MKRSAPTPTPKQPNQRLRELTEELRELEEKLRLGGGPEKIEKQH
                     QQGKLTARERVALLLDEGAFAQEIGLLVAYDQYDGTAPGAGVVTTVGRVEGREVVVVA
                     NDATVKAGSWWPETIKKILRAQEIAMRSHVPIIYLVDSAGVNLPYQGGVFPGQYGASR
                     IFYYNSLMRRYLKIPQISAVMGPCIAGGAYLPALSDVIVMVEGTSFMGLGGANLVKGA
                     TGQTIDNETLGGARAHNEISGVAHYRVKNDQECVAKIREFVSELALNPPSPVVILPSE
                     EAARAADELYEIIPEDHKQPYNVRHLLDCLLDAGHLDEFQADYAKEMITGHARIRGIQ
                     VGIIANNRGMIRAPGGGAPRFGGIIYTESAEKVAFFIDTCNRRHTPLLFIQDVSGFMV
                     GPEAEHSGIIRAGAHFVEAMATALVPKIVLTVNHASGAGYYAMAGQGFDPDFIFSWPT
                     GRMGVMEGDSAVQAVFGSQLEKLKRNDLGPSEQLSAEMDAVRDTYATELDAKYAAARG
                     FVDAVITPDATRAALELALRVGLNYSGPHIGQFVLTNWS"
     gene            2767..4134
                     /locus_tag="VFV61_11130"
     CDS             2767..4134
                     /locus_tag="VFV61_11130"
                     /inference="COORDINATES: protein motif:HMM:NF018938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HEX4901076.1"
                     /translation="MKSIVRIAAGQGFWGDLLDAPVRQVEGGPIDYMMLDYLAEVTMS
                     IMQKQRSRDPLAGYAKDFVPLMKQILPACVERNIRVTANAGGVNVEGCADAVREVARE
                     LGLAGKVEIGIVTGDNIMPRLDELLDKGIELRNMDTNETLSEVRDRIQSANAYLGAWP
                     MVEALHRGARIVITGRATDTGLTLAPLIHEFGWKADDWNMMAAGTIAGHIIECGAQCS
                     GGNCQYDWRSIPNLAEVGFPIVEAAPDGTFVVTKHENTGGCVGIPSVKEQLVYEMGDP
                     HEYITPDCIADFTSIRLEDAGPNRVRVYGIEGRPATEFLKVSISYSAGFKAVGTLVYS
                     WPDAYEKAQLADKILRARLERLGLRFDQVLTEFVGANATHGSLAGEPRSDLPEVQLRV
                     AVRGEDRKAVERFTKEIAPLILTGPPGVTGFAGGRPKVEEIVAFWPALMPKSEITPQV
                     EIIEA"
     gene            4231..>4576
                     /locus_tag="VFV61_11135"
     CDS             4231..>4576
                     /locus_tag="VFV61_11135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4901077.1"
                     /translation="MQIQLTKLAHARSGDKGDTANVGLIALRDEFYPILVREVTAASV
                     KQHFGEMVKGNVERFELPNLGALNFLLHESLGGGGTLSLMTDAQGKTFSTGLLRMSIE
                     VPDDEARRLGLIQ"
BASE COUNT         1077 a         1135 c         1346 g         1018 t
ORIGIN      
        1 actatcagaa atgataaaac gcagactctg gtccgactgc tgattagtgg catgcgttac
       61 ttataggtac cgttctttca agatcgccag gtggtgagcc gagtggccgg cgatgatgta
      121 ggcgagcgcc cgcaccgtaa agggattacc gctggcaatg ccagagcggt cccagacgtt
      181 ctgtgactct gcgaggtttt ctgccggcaa attgcggaag aaggcaagcg ttgccaggcg
      241 aatcgcgcga aactctgcga tgtgatttgc ccagtcaaac tcgtcagctt gcgctccagc
      301 gacgccaacg tcctggtcgt aactgggaag gtcctcggcg aagccccgag cgaaccagag
      361 ggcgcgaaac agaaagacgc gttccgcgtc gttaacgtga ttgagcagtt cgcgcatcga
      421 ccacttctcg ggcgcataac gatgcaagga gtgctcttca gaaatctcgc ttaaaaaagc
      481 gacagtttca tcaagctgac gttcaagtac agcgacaaca tcgtcctcag tgacgcggtt
      541 gatgtagcca tgatagtagg atgcggcttc gtttggttgc ggacgcatag tatgggaatc
      601 gtaaaacagg gtcagcaaaa cggcaaacct ggcgagagtt tttccatctc tctatcaaaa
      661 acttaagcgc agagacgcga cgaaaacagg acaaccaaaa gccacttttg atgagactac
      721 ccaaccaagt tcgtgatgtt tggtttggtt tagttgattt gatctcctgc ggctatcgcg
      781 aacgatccaa gaagtgacac gaaccatcac gaagcatgtt gataggttcc ttgtggctct
      841 gcgcttttgc gtctctgtgc tttaggtttt gtcgaatcaa aatatcaaat gataggggag
      901 aatcatctgg actttcgctt gttccaggtc cctgaggttg ggttcctcaa attgtcaccg
      961 tctctcccat catgttactt tgattccaga cagctattct tttgaatcat gaagcgctcc
     1021 gccccgacac ccactcctaa acaaccgaat cagcgtttgc gagagttaac cgaagagttg
     1081 cgggagctcg aagagaaact gcggcttggt ggcggtccag agaaaatcga gaaacagcat
     1141 cagcaaggca aactcacggc ccgcgaacgc gtcgctctcc tcctcgacga aggagcattt
     1201 gcgcaggaga tcggattact tgtcgcctac gatcaatatg acggaaccgc tccgggcgcc
     1261 ggagtggtaa ctacagtggg ccgagtcgag ggcagggaag ttgtggtggt ggcaaatgat
     1321 gccacggtaa aagcgggttc gtggtggccc gagacgatta agaagattct gcgagcgcag
     1381 gagatcgcga tgcgttctca tgtgccgatc atttacctag tcgactcagc gggcgtgaac
     1441 ctgccctacc agggcggtgt ctttcccggt caatacgggg cctcgcgtat cttctattac
     1501 aactcgctga tgcggcgcta tctcaagatc ccgcaaatct ccgcggtgat gggaccgtgt
     1561 atcgccggtg gagcttatct accagcattg tctgacgtga ttgtcatggt cgaaggcacg
     1621 tcgttcatgg ggctcggcgg cgcgaacctg gttaaaggtg cgaccggcca aacgatcgac
     1681 aacgaaacgt tgggtggcgc tcgtgctcac aatgagattt ctggagtcgc gcactatcgc
     1741 gtgaagaacg accaggaatg cgtcgcgaaa attcgggagt tcgtttccga gctcgccttg
     1801 aatcctccca gtccggtcgt aatcctgccc agcgaagaag ctgcaagagc ggctgatgag
     1861 ttatacgaaa taatccccga agatcataaa cagccgtaca acgttcgcca tctactcgat
     1921 tgtctcctcg acgccggcca tcttgatgaa ttccaggcgg actacgcgaa ggaaatgatc
     1981 accggtcacg cacgcatccg tggcattcaa gtaggaatca tcgccaataa tcgcggcatg
     2041 attcgcgcgc ctggcggcgg cgcaccacgt ttcggtggca ttatttatac ggagtccgcg
     2101 gagaaggtgg cgttcttcat cgacacctgt aatagacgtc acacgccgtt actatttatt
     2161 caggatgttt cggggtttat ggtgggaccc gaagctgaac attcgggaat cattcgcgct
     2221 ggggcgcact tcgtcgaggc gatggccacg gcgttagtgc ctaagattgt gctgacagtc
     2281 aatcacgctt ccggggcagg gtactacgcg atggccggtc agggttttga tccggacttt
     2341 attttcagtt ggccgacagg acgcatgggc gtgatggaag gcgactccgc agtgcaggcg
     2401 gtctttggta gtcaactcga gaaactgaag cgtaatgatc tgggaccaag cgagcagctc
     2461 agtgccgaga tggatgcagt acgcgacacc tacgcaacgg agcttgatgc gaagtatgcg
     2521 gcggctcgtg gttttgtcga cgcggtgatc acgcccgacg caacccgcgc tgccttggag
     2581 ctcgccttgc gcgtaggttt gaattacagc gggccgcaca ttgggcagtt tgtactaaca
     2641 aattggtctt aggtcttggg cttgggtctt tgatcgctgt catctgaagt aacaacgtca
     2701 gtggcgaaaa gaccaaagac caaaggtcaa aagccaaaag ccgaaaaacc aagtctagaa
     2761 ttatcaatga aatctatcgt acgtatagct gctggccagg gcttctgggg cgacttgctg
     2821 gatgcacccg tgcgtcaggt tgaaggcggc ccgattgatt acatgatgct cgattaccta
     2881 gccgaggtaa ccatgtcgat catgcagaag cagcgttcgc gcgatccgct tgccggctat
     2941 gcgaaggatt tcgttccctt gatgaagcaa atcctgccag cctgtgttga gcgcaacatt
     3001 cgtgtgactg caaacgctgg aggcgtcaat gttgaaggtt gcgctgatgc ggtgcgggaa
     3061 gtcgcgcgcg aacttggcct ggcgggcaaa gtagagatcg gcatcgttac cggcgacaac
     3121 ataatgccgc gcctcgatga gctgctcgac aaagggatcg agttgcgcaa catggacacg
     3181 aacgaaacgt tgtctgaggt tcgcgatcga atccaaagtg ccaatgccta tttgggcgcc
     3241 tggccaatgg tggaagcgct gcatcgcgga gcgcgaatcg tcattaccgg cagagcaaca
     3301 gacacggggt tgacacttgc gccactgatt catgagtttg gttggaaagc ggacgactgg
     3361 aacatgatgg cggcgggaac aattgctggc cacattatcg agtgtggcgc gcagtgctcc
     3421 ggcgggaatt gccaatacga ctggcgtagc attcccaatc tcgccgaggt tgggtttcct
     3481 attgttgagg ccgcaccgga cggcacattc gtggttacta aacatgagaa tacgggtggt
     3541 tgtgtgggga tcccttccgt caaagaacaa ctggtttacg aaatgggcga tccgcacgaa
     3601 tacatcacac ccgactgcat tgccgacttc acttcgatca ggctggaaga tgcgggaccc
     3661 aatcgcgtgc gtgtttatgg gattgaagga agaccagcta ccgaattcct taaggtatcg
     3721 ataagttatt cggctggctt caaagccgtg gggacgttgg tgtactcctg gcccgatgcg
     3781 tacgagaagg cgcaacttgc cgacaagatt ttgcgcgcgc gtctggaacg actggggctg
     3841 cgcttcgatc aagtgttgac tgagttcgta ggtgcgaatg cgacgcatgg ctcgttggca
     3901 ggcgagcctc gcagtgacct gcctgaggtg caattgcgtg tggctgtacg aggtgaggat
     3961 cgaaaggccg tggaaagatt cacgaaagag attgcaccgt tgattcttac cggcccgccg
     4021 ggggttacag gttttgctgg tgggcggccc aaggttgaag agattgttgc cttttggccg
     4081 gcactgatgc ctaagagcga gattactccg caggttgaga tcatcgaagc ttaggagtag
     4141 ccacagataa acgctgtcta cagggatcag aagatcaaaa gcaggaagcc gagcgagcaa
     4201 agaccaaagc caaagaccaa agacccttca atgcagatcc aattaacaaa acttgcgcat
     4261 gcccgttccg gagataaagg cgacactgcg aacgtcggtt tgatcgcctt gcgcgatgag
     4321 ttctatccga tcctggtgcg tgaagttact gccgcgagcg taaagcaaca tttcggggag
     4381 atggtcaaag ggaacgtcga gaggttcgag ttaccgaatc tcggtgccct caattttctg
     4441 ttgcacgaga gtctcggtgg cggcgggacg ctttcattaa tgactgatgc ccagggaaaa
     4501 acgttttcta caggtctgct gcgcatgtcg attgaagtcc ccgacgacga ggcgcgccgc
     4561 cttggattga ttcagg
//
LOCUS       DASUEX010000672         4988 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_679868, whole genome shotgun sequence.
ACCESSION   DASUEX010000672 DASUEX010000000
VERSION     DASUEX010000672.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4988
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_679868"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(297..1034)
                     /locus_tag="VFV61_11140"
     CDS             complement(297..1034)
                     /locus_tag="VFV61_11140"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEX4901078.1"
                     /translation="MNNERVGSNFLAAAVLAAGLVLAAIIFGYAFVKAKQGDQTITVT
                     GSARKRIKSDLVIWKAGVSYQAPVLADAYRSLSENVPRVKAYLLGKGIPENQITISSI
                     SSQTLHARDADGQDTGQITGYSLRQELEVRSNDVDLIARVAREATELINQGILLESSA
                     PEYHYTKLGEEKITMLAEAAKDAKKRAEQVAASTESSIGSVRTARMGVLQITPADSNE
                     VSDSGMNDTSSLDKDITAVVNLSFEIN"
     gene            1291..3468
                     /locus_tag="VFV61_11145"
     CDS             1291..3468
                     /locus_tag="VFV61_11145"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX4901079.1"
                     /translation="MCLSRRGWRSAAVSLVITGVFALIALPSAQAQNATAGAAQNMIK
                     SLTYPTARKGDQVDDYHGIKVADPYRWLEDLDSEETRNWVEAENRLTSSFLNEIPARA
                     RIRERLTKLWNYERFGVPFREGKNYFYTRNSGLQNQAVLYTVTSLEGQPTLLLDPNTL
                     STDGTVALSGTAVSNDGKLLAYSLSASGSDWQEWKVRDVSTGKDLADNIKWVKFSGAS
                     WTADNQGFFYSRYDEPKSDSLKGTNYFQKLYYHKLGTPQSEDVLIYERPDQKDWLFGG
                     NVTEDGRYLIISVFQGTDNKNRVYYKDLQAKDAQVVKLLDDFDAAYNFVGNEGTVFWF
                     QTDLKASRGKIIAIDVSKPDRGSWQVLVPEAAEALQGVNVVNHMFVANYLKDAHTQVK
                     IFSTSGKFVREVAFPGLGTAGGFGGHAKDKETFYSYTGFTTPTSIYRYDMVTGKSSIF
                     RQPKADFNSEDYETKQVFYPSKDGTKVPMFITYKKGLKLDGNNPTYLYGYGGFNISLP
                     PAFSVSNLVWMEMGGVYAQANLRGGSEYGEDWHQGGMKLKKQNVFDDFIAAAEWLIEN
                     KYTSTPKLAIGGGSNGGLLVGAVMTQRPDLFGAALPAVGVMDMLRFQKFTIGWAWVSD
                     YGSSDNAEEFKALYAYSPLHNIKSGKVYPPTMVTTADHDDRVWPGHSFKFAAALQEAQ
                     AGAAPILIRIETKAGHGAGKPTSKVIEEVADKWAFLRRTLNVE"
     gene            complement(3570..4448)
                     /locus_tag="VFV61_11150"
     CDS             complement(3570..4448)
                     /locus_tag="VFV61_11150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4901080.1"
                     /translation="MHITVSTPPTFNFKRTIISHGWCELLPFAIDREQWTLTRTLDLG
                     DTTPITISLSATKRAVQINTSRTISKQATDTVRNQVRHILRLDDDLKPFYRTMKGDPE
                     FQWVSDEGAGRMLRSPTVFEDLVKMICTTNCSWALTEKMVTGLVENLGRKSSNSHRTF
                     PTPEAMALMPEKFYVKEVRAGYRAGYLKELADRVASGELNVESWLNSNLPTPALLKEM
                     KGVKGVGDYAAENLLKLLGRYDGLALDSWTRSKFYSVRNNGRKAADKKIARYYSRFNE
                     WRGLALWCDMTRDWIE"
     gene            complement(4515..>4988)
                     /locus_tag="VFV61_11155"
     CDS             complement(4515..>4988)
                     /locus_tag="VFV61_11155"
                     /inference="COORDINATES: protein motif:HMM:NF015436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HEX4901081.1"
                     /translation="KHMQSSHEHLSIVVDEHGGMEGIITLEDLLEEIVGEIDDEYDEE
                     VRSQISRESDGSYLLSGMLTVRDANRQLDLNLPEDGGYITLAGFLLAQAGRLLYEGET
                     VKYEGMCFKVEQVDRRRIRRVRLNLQEAGFVSKLGLVPLISCAVNESFEIAPLLL"
BASE COUNT         1191 a         1322 c         1300 g         1175 t
ORIGIN      
        1 aacgagggaa gcagatctca tgattgagtt aactctcgga ttgtaaaact cctcgctggg
       61 gggcagcgag tgtgtcttag attcgctgat gccgcgtgtg gttgtcaacg attatcggat
      121 tgcctgggga gagcctaacc gcctaatcaa gaaacgcaga gagcgccaag aagaacagac
      181 tggcttagta cttttgttct tagtcctctg ttcttatggc tcggcataac tttgcctctc
      241 aagtttcatc agcgaacaaa gttcaaagaa ccaggttcaa agctcgtttt gcggaatcaa
      301 ttaatctcaa aactgaggtt cacaactgcc gtaatgtcct tatcgagaga tgaagtatcg
      361 ttcatgccgc tgtctgaaac ttcattggag tcggcaggcg taatctgcag cacccccata
      421 cgcgctgtgc gcacgctacc aatgctgctc tcggtgcttg ccgcaacttg ttcggcccgc
      481 ttttttgcgt ccttcgccgc ttcggcaagc attgtgatct tttcttctcc gagcttggtg
      541 tagtgatact ccggcgcact ggactccagc agaatgccct ggttgatcag ttccgttgcc
      601 tcgcgggcga ctctcgcaat taggtctaca tcgttggaac gcacctcaag ttcctggcgc
      661 agagagtaac cggtgatctg tccggtgtcc tgaccatcag catcgcgagc atgcagcgtt
      721 tgggaggaga ttgaagatat cgtgatctgg ttttccggga tgcctttacc gaggagataa
      781 gccttcacgc gcggaacgtt ctccgacaag gagcgatagg catcagcgag cactggggct
      841 tgatacgaaa ctccagcctt ccaaataacc agatctgact tgatccgttt ccgcgccgag
      901 ccggtgacgg tgattgtctg atcaccctgt ttggccttca cgaaagcgta gccgaagatg
      961 atcgcggcca gcaccaggcc cgcggccagc accgcggcgg ctaaaaaatt agatccgact
     1021 cgttcgttat tcatttgtgg gggaactcct taaaccaaga attgcggctg gacaatacaa
     1081 cgaactcacg aaacaactca agaagatcgg gaaaagttaa gagtaaccgc caaggcggtt
     1141 tcagttccga tgaaaaatga catatggaaa atgtctttgc cgtttcctgg cggcttggcg
     1201 gtcaagtttt ttggggtata cttcggcttg ttcaaggtct gatacatctc gtctcccccc
     1261 aatctcttcc caagtgaaag gacagttctt atgtgtctct caaggcgtgg gtggcgttcc
     1321 gccgcggtct ctcttgtcat caccggcgtg tttgctttga tcgcgctccc atcagcccaa
     1381 gcgcaaaacg caactgccgg cgcagctcag aacatgatca agtccctgac ctatccgacc
     1441 gcgagaaaag gagatcaggt ggacgattat cacggcatca aggtcgctga cccttatcgc
     1501 tggcttgagg acctggactc cgaggagaca cgaaattggg ttgaggcaga aaacagactg
     1561 acgtccagtt ttcttaatga gattcctgcg cgggccagga tccgggaacg cctgactaag
     1621 ctctggaact atgaacgttt tggcgtgccg tttcgcgagg gcaagaacta tttctacacc
     1681 cgcaacagcg gcctacaaaa tcaagcggtg ctctacaccg ttacttccct cgaggggcag
     1741 ccgaccctgc tccttgatcc caataccctt tctactgatg gcactgtggc tttgtcggga
     1801 actgcagtca gcaacgatgg caagcttttg gcttacagcc tttcagcttc agggtctgac
     1861 tggcaggagt ggaaggtgcg cgatgtcagc accgggaaag atcttgccga caacatcaaa
     1921 tgggtgaagt tttccggcgc gtcatggacc gccgacaacc agggtttctt ctacagtcgt
     1981 tatgatgaac cgaagagcga ctcactcaaa ggcacaaact actttcaaaa actctactac
     2041 cacaaacttg gaacgccgca gtcagaagac gttttgattt acgagcggcc ggatcagaag
     2101 gattggctct ttggcggcaa cgtcactgaa gacggacgct acctgatcat ctcggtcttc
     2161 cagggcaccg ataataagaa ccgcgtctat tacaaagacc tgcaagcaaa agatgcgcaa
     2221 gtggtcaaac tacttgacga cttcgacgcc gcttacaatt tcgtcggcaa tgaaggaact
     2281 gtgttttggt ttcaaaccga tctgaaagct tcgcgcggca agatcattgc gattgatgtc
     2341 agtaaaccgg accgcggcag ttggcaggtc ctggttcccg aagctgcgga agcgcttcag
     2401 ggtgtgaacg tcgtgaatca catgttcgtt gctaactacc ttaaggacgc ccacacgcag
     2461 gtaaagattt tcagcaccag cggaaaattt gtgcgcgaag tggccttccc gggattggga
     2521 acggccggcg gtttcggcgg acacgctaag gataaggaga ccttctactc gtacaccggg
     2581 ttcacgacgc cgaccagcat ttatcgttac gacatggtta cggggaagag ctcgatcttt
     2641 cgccagccaa aagcagactt caattcggaa gactacgaaa ccaaacaggt tttctacccg
     2701 agcaaagacg gcacgaaagt gccgatgttc attacgtata agaagggcct gaaactcgac
     2761 ggcaataacc caacctacct ttacggttac ggtggcttca acatctcgct gcccccggcg
     2821 ttttccgtca gcaacctggt gtggatggag atgggcggag tctatgctca agcgaatctc
     2881 cgtggtggca gcgagtatgg cgaagactgg caccagggcg gcatgaagct aaagaagcag
     2941 aacgtctttg acgactttat cgcggctgcg gaatggctca tcgagaacaa gtacacgtca
     3001 acgcctaagt tggcgattgg tggtggcagt aatggcgggt tgttagtagg agccgtaatg
     3061 acgcagcgac cggatctatt cggcgccgca ctgccggcgg taggcgtgat ggacatgctc
     3121 cgctttcaga agttcacaat tggctgggcc tgggtttccg attatggctc gtcagacaat
     3181 gcagaggagt tcaaagctct ctatgcttat tcgcctttgc acaacatcaa atcaggtaag
     3241 gtctatccgc cgacgatggt gaccaccgcc gatcacgacg atcgcgtctg gccgggacac
     3301 agcttcaaat ttgcggctgc cttgcaggag gcgcaggcgg gagcggcgcc aatcttgatt
     3361 cggatcgaaa cgaaggcggg acacggcgcg ggaaaaccaa cttcgaaggt tattgaggaa
     3421 gtcgccgaca agtgggcgtt cttacgaagg accttgaatg tcgaatagac ttaaaggtct
     3481 caggtctcaa gtctcaagtc tcaggtctcc aagtctcagg tctcaagtcc caattgttct
     3541 taacttgaga cttgggacct gagactttct tattcaatcc agtctcttgt catgtcacac
     3601 cagagcgcga ggccgcgcca ttcattaaac cgagaatagt agcgcgcgat ttttttatca
     3661 gccgccttac gtccgttgtt ccgtaccgaa taaaactttg agcgcgtcca cgaatcgagc
     3721 gccaaaccgt cgtatcggcc caacaactta agcaggtttt ccgccgcgta gtcgccaact
     3781 cccttcacgc ctttcatctc tttgagcaaa gccggagtag gcaaattact gttcaaccag
     3841 gactcaacat tcaactcgcc tgacgcaaca cgatccgcca attctttaag gtacccggcg
     3901 cggtaaccag cgcgcacttc ctttacgtaa aatttctccg gcatcagggc catcgcttcg
     3961 ggagtaggga atgtccggtg gctattgctc gacttgcgcc ccaggttttc caccaagccg
     4021 gtcaccatct tttccgtcag cgcccaggag caatttgtcg tacaaatcat cttcaccagg
     4081 tcttcaaata ctgtgggcga acgcagcatc cgcccggcgc cctcgtccga tacccactga
     4141 aactccgggt cgcccttcat cgtgcgatag aacggtttca gatcgtcatc aagccgcagg
     4201 atgtgacgca cctggttgcg gacggtatct gtagcctgct tgctgattgt gcgcgaagtg
     4261 ttgatttgga ccgcgcgttt ggttgccgag agcgagatgg tgattggcgt tgtgtctccg
     4321 aggtcaagag ttcgcgtcaa agtccactgc tcacggtcga tggcaaaggg caatagttca
     4381 caccagccgt gactgatgat ggtccgcttg aaattgaaag tcggcggggt agagacagtt
     4441 atgtgcatga ttaccttaat ctggattctt agaatacgaa taaggtgtga gagtaagcaa
     4501 acgcaagggc tctatcaaag caggaggggc gcaatctcga aagattcatt aacggcgcag
     4561 ctgatcaaag gaaccaatcc aagtttggat acaaatccag cttcctggag attcaaccga
     4621 actcggcgta ttcgtcgcct gtctacttgc tcgaccttga agcacattcc ctcatacttc
     4681 acagtttcac cctcgtacag tagtcgcccc gcttgcgcca ggagaaagcc ggcgagagtt
     4741 atataaccac catcttcagg gaggtttaga tcgagctggc ggttggcatc gcgcaccgtc
     4801 agcattccac tcaataaata cgagccgtcg ctttcccttg atatttgcga acgcacctct
     4861 tcatcatatt catcgtcaat ctcaccaacg atctcttcca acaaatcctc cagtgtgatg
     4921 atgccctcca tgccgccgtg ctcatccacg acaatcgata aatgttcgtg cgacgactgc
     4981 atatgttt
//
LOCUS       DASUEX010000673         4082 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_555962, whole genome shotgun sequence.
ACCESSION   DASUEX010000673 DASUEX010000000
VERSION     DASUEX010000673.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4082
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_555962"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="VFV61_11160"
     CDS             <1..425
                     /locus_tag="VFV61_11160"
                     /inference="COORDINATES: protein motif:HMM:NF012803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEX4901082.1"
                     /translation="YHKPSDTADKINYEDESRILGVVAKIVRSIDAGAKRPTYMVAKT
                     EATGRSTGFRVSLGTIPNYSDSGDGLLLDGVREDSPAAKAGLKAGDRIVRLAGRDVRN
                     VYDYTYVLGEMKAGQEYEVEVMRGTERLKFKLTPAARR"
     gene            594..2048
                     /locus_tag="VFV61_11165"
     CDS             594..2048
                     /locus_tag="VFV61_11165"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF006673.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEX4901083.1"
                     /translation="MSKTVPITVANGDGIGPEIMGATLEILKGAGAALEIEMIDIGEK
                     VYLAGNSSGIEPSSWDSLRRTKVFLKAPITTPQGGGFKSLNVTVRKTLGLYANVRPCV
                     SYHPFVKTKHPNMDVVIVRENEEDLYAGIEHRQTAQVMQCLKLISRPGSEKIVRYAFE
                     YARRNNRKKVTCFTKDNIMKLTDGLFHKVFDEIAAQYPDIENEHWIVDIGAAKLADTP
                     EAFDVLVMPNLYGDVLSDVAAQIAGSVGLAGSANIGEHVSMFEAIHGSAPRRAGQNLA
                     NPSGLLLGAVMMLVHIGQTDVAERVHNAWLRTIEDGIHTYDVYKEGVSREKVGTKEFA
                     AAVVARVGQVPQTLKASKYQAAPESAAAKPSSLATPAPKKELVGVDVFLDWTSGSAND
                     LGESLQKLGDDGLKLEMMSNRGVKVWPGGHAETFCSDHWRCRFLSGKVDGSLSHDQIV
                     ALLNRVASAGFDFIKTENLYNFDGERGFSLDQGA"
     gene            2126..2704
                     /gene="purE"
                     /locus_tag="VFV61_11170"
     CDS             2126..2704
                     /gene="purE"
                     /locus_tag="VFV61_11170"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEX4901084.1"
                     /translation="MTTKTKDRTKRKSSGSPRPPISPSPRPRVPASPRPLVAVIMGSK
                     SDWETMRHADQTLTDFAVAHECKVMSAHRTPRLAADFASNAESRGIEIIIAAAGGAAH
                     LAGVLAAHTTLPVLGVPMKSDALNGMDSLLATVQMPAGIPVGTLAIGKPGAVNAALLA
                     VAILANQRPQLRQQLKQFREKQEQQVRAATLD"
     gene            2855..>4082
                     /locus_tag="VFV61_11175"
     CDS             2855..>4082
                     /locus_tag="VFV61_11175"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX4901085.1"
                     /translation="MIGQTISHYRIIEPLGEGGMGTVYLAEDTHLGRQVAIKFLSELD
                     PHYRARFLREARAVSALSHPHIAAVYDYGETRDGQPYIVMELVKGRTLSDLLEDESLS
                     LASSVQIVSEIAEALGEAHRLGIIHRDVKPANVVINERGQVKVLDFGLVKQLAKDDTG
                     KADARKSFAYTQSDVIVGTPLYLSPEQATGKAVDGRSDLFALGALLYECITGKSAFSG
                     TSVIEIGAQVIHVTPPLPSQTNPRIPTELDRVTMKALQKDPDARHQSAAELIAELNAV
                     AASLPSDGDYPPRAPASVARASALTTLTQTLRRPRLSLGAFLIGAIIVAFIVWAAMRY
                     WKPAPYQPTPEALTWYNKGVDALRNGAFLQASKTLEQAATADPDFALAHARLSEAYTE
                     LDYTDKAKDELLRVSSL"
BASE COUNT          947 a         1119 c         1155 g          861 t
ORIGIN      
        1 actatcacaa accatcggac acggctgaca agatcaacta cgaggacgag tcgcgcatcc
       61 ttggggtcgt cgcaaagatt gttcgcagta ttgatgccgg cgctaagcga ccgacctaca
      121 tggttgccaa gacggaagca accggacgtt cgacgggatt tcgcgtatcg ttgggcacga
      181 tccccaacta ctcggacagt ggggacggtc tattgctgga tggcgtgcgc gaagattcac
      241 cggcagccaa agcgggtttg aaggcaggcg atcggatcgt aagacttgcc ggccgtgacg
      301 tgcgcaacgt ttatgactac acctacgtgc tgggtgaaat gaaggcaggt caggaatacg
      361 aagtggaagt catgcgcggg acagagcgac ttaagttcaa gttaacgccc gcagcgcgaa
      421 ggtgaagaga ctgccgtttg ccgattgtcg attggcgctt cacttagcag tctcgtatta
      481 ttctatccac taaatcttaa cggtgttttg tttcaattgg cgatcgacag ttgtgaattg
      541 gaaacgattc caatcggcaa ttggcaatcg acaatcggca attttggagg actatgtcta
      601 aaactgttcc gattaccgtc gcaaacggcg acggcattgg ccccgagatc atgggcgcca
      661 cactcgagat tctcaaaggc gcaggtgcgg cgctggaaat cgaaatgatc gacattggcg
      721 agaaggttta tcttgccggc aactcttcgg gaatcgagcc gagctcctgg gattcattac
      781 gccgcaccaa agttttcttg aaggcgccga tcaccacacc acaaggcggt ggctttaaaa
      841 gtctcaacgt aacggtccgc aagacgcttg gcctttatgc caacgtgcgc ccttgcgtgt
      901 cttatcaccc gttcgtgaag acaaaacatc cgaacatgga tgtcgtgatt gtccgtgaga
      961 atgaagagga tctgtacgcc gggatcgaac accgccagac ggcgcaggta atgcaatgtc
     1021 tgaaactcat ttcccggcct ggttcggaga agattgttcg ttacgcgttc gagtacgcgc
     1081 gccgcaacaa tcgcaagaag gtcacgtgct tcacgaaaga taacatcatg aagctgaccg
     1141 acggtctttt tcacaaagtt tttgacgaga ttgcggcgca gtatcccgac atcgaaaacg
     1201 aacactggat cgtcgacatt ggcgctgcca agctcgccga cacgcccgaa gcgtttgatg
     1261 tcctggtgat gccgaatctt tacggcgacg tcttgtcaga cgtggcggcg cagatcgcgg
     1321 gctcagttgg gttggcgggt tccgctaata tcggcgaaca cgtctcgatg ttcgaagcaa
     1381 ttcatggatc ggctccgcgc cgcgccggac aaaatctggc gaacccgtca ggattactgc
     1441 tcggcgccgt gatgatgctg gtgcacatcg ggcaaacgga tgtcgctgaa cgagtacaca
     1501 atgcctggtt acgcaccatc gaagacggga tccacaccta cgatgtttat aaggaaggcg
     1561 tgagccgcga gaaggtcggc acgaaagagt ttgccgcagc cgtggtcgcg cgcgtgggac
     1621 aagtgccgca aacgttaaag gcgtcaaagt atcaggccgc gccggagagt gctgcggcga
     1681 agccgagctc cctggcgacg ccggcgccga agaaggaact cgtgggagtg gatgtatttc
     1741 ttgattggac tagcgggtcg gcaaacgatc taggcgagtc gttgcagaag ctgggagacg
     1801 atggcctcaa actcgagatg atgagtaatc gaggtgtgaa agtctggccg ggtggacatg
     1861 cggaaacgtt ttgctcggat cactggcgct gccgcttctt gtcaggaaag gtcgacggtt
     1921 cactctctca tgatcagatt gtggcgctgc ttaatcgagt cgcgagtgct ggtttcgatt
     1981 tcatcaagac cgaaaacctt tacaactttg atggtgaacg cggcttctcg cttgatcagg
     2041 gagcgtaggg cgagacacgg agacacggag acacggagac gcggggacac ggggacacgg
     2101 ggacacgggg atggggagaa cagacatgac tacgaaaaca aaagaccgaa ccaaacgtaa
     2161 atcttccggc tcgccgcgtc cccccatctc cccatctccg cgtccccgcg tccccgcgtc
     2221 accccgtcct ctcgtcgcgg taatcatggg aagcaagtct gattgggaaa ccatgcgtca
     2281 cgcagatcag accctcactg attttgcggt agctcacgaa tgcaaggtga tgtcggcaca
     2341 ccgcacaccc cgcctcgccg ctgactttgc ttccaatgcg gagtcgcgcg gtatcgagat
     2401 catcatcgct gccgctggtg gcgccgccca tctcgcgggt gtacttgctg cgcacacgac
     2461 tttgcccgtg ctcggcgtac caatgaaaag tgatgctctc aacggtatgg attctttgct
     2521 cgctaccgtg caaatgcccg ccggaatccc tgtcggcacc ctcgcgattg gaaaacctgg
     2581 cgccgtcaat gctgcgctcc tggccgtcgc aattctggca aatcaacgtc cgcaattgcg
     2641 ccaacaactg aaacagtttc gtgaaaagca ggagcaacaa gtgcgcgccg caaccctcga
     2701 ttagcgacca attcacgcct taagttgttc ttgtgttccc caccgcttta gagcaaaatg
     2761 ccgccattgg atcgagcttt tcgaattcgt ttgaatttca gacgacctct ctgttagcac
     2821 ttcttctcca ctcgcgtttt gaaggaaatt caaagtgatc ggtcaaacga tctcccatta
     2881 ccggatcatt gaaccgctcg gtgagggtgg aatgggcact gtttatctgg ccgaagatac
     2941 tcatctcgga cgccaggtgg ccatcaagtt tctctccgaa ctcgatccac actaccgtgc
     3001 gcggtttctc cgcgaagccc gggccgtgtc ggcgttgagc catccgcaca tcgccgccgt
     3061 ttatgactac ggcgaaacca gggacggcca gccctacatt gtgatggagt tggtgaaggg
     3121 taggaccttg agcgatttgc ttgaagacga aagtttgtcg cttgcaagct cagtgcagat
     3181 cgtctcagag attgccgagg cattgggtga agcgcaccgg ctcggaatta ttcaccgtga
     3241 tgtgaagccg gcgaatgttg ttatcaacga acgtggtcag gtaaaggtgc ttgattttgg
     3301 tcttgtcaaa cagcttgcca aggacgacac cggcaaggca gacgcgcgaa aaagctttgc
     3361 ctacacccaa agcgatgtga tcgtcggaac tccactgtat ctttcgccgg aacaagctac
     3421 gggaaaagct gttgatggtc gcagcgatct attcgcgctg ggcgccttgc tctatgaatg
     3481 catcaccggc aagtcagcct tttccgggac cagcgtgatc gagattggcg cgcaggtcat
     3541 tcacgtcacg ccaccactgc cttcacaaac taacccacga atcccaaccg aactggatcg
     3601 cgtgacaatg aaagcgctcc agaaagatcc cgatgcgcgg caccagtccg cagcagaact
     3661 gatcgcggaa ctgaacgcag tcgcggccag tcttcccagc gatggagact atccgccgcg
     3721 ggcgccggcg tcggtcgcgc gtgccagtgc tttaacaaca ctgacccaaa ccttgaggcg
     3781 ccctcgacta tcgctgggcg cctttcttat tggcgcgatt atcgttgcct tcatcgtctg
     3841 ggcggcgatg cgctactgga aaccggcccc ttaccaacca actccagagg cattaacctg
     3901 gtacaacaag ggcgtcgacg cgctgcgcaa cggcgcgttt cttcaggcaa gcaaaacgtt
     3961 ggaacaagct gccacagcag acccggactt tgcactcgcc cacgcccgac tgtcggaagc
     4021 ctatacggaa ctcgattaca ccgacaaagc aaaggacgaa ttgttgcgtg tgagttcgct
     4081 ag
//
LOCUS       DASUEX010000674         3858 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_556319, whole genome shotgun sequence.
ACCESSION   DASUEX010000674 DASUEX010000000
VERSION     DASUEX010000674.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3858
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_556319"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1038
                     /locus_tag="VFV61_11180"
     CDS             <1..1038
                     /locus_tag="VFV61_11180"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX4901086.1"
                     /translation="RIHLLRWWALVIVTAITLYLCWLMLQPFVKVISWAAVLVIVFYP
                     VHKQILRRTRKPALSALVSCALVILVILVPVALITLAVVSEMSGAMQNLQSAITYVLD
                     PNSRITGPILGWLGHYLDLSRLQTDEYLLTQLQSMSGSIAGRTLGFLGGLISVVVQMV
                     FVIFTMYYFFKDGEKILTAVRDVLPLERREAEGVMARTREVIDASVYGVITIAIIQGA
                     LGGLAFWLLGLPSAIIWGVAMSFFSMIPILGAFVVWVPAAIYLASTGHWGKALMLVLW
                     GTLVIGTIDNFLRPKLVGSRTRLHELLIFFSVLGGLQVFGVLGVVLGPVVVAIALALK
                     DVFQKASHETA"
     gene            complement(1060..1758)
                     /locus_tag="VFV61_11185"
     CDS             complement(1060..1758)
                     /locus_tag="VFV61_11185"
                     /inference="COORDINATES: protein motif:HMM:NF014090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HEX4901087.1"
                     /translation="MPQTPHTERHFTASEVVRDVVIGMSDGLTVPFALAAGLSGAIVQ
                     TNIIVTAGLAEIAAGAIAMGLGGYLAAKSDAEHYAKEREREKREVAEIPHEEMREVAE
                     VFQEYGLSAEETEPIVQALRRQPKKWIDFMMRFELGLERPNPKRAMTSALTIGGAYVA
                     GGLVPLAPYLFIPVAPRALLYSIAVTLVALFVFGYVKGRFTGMRPLRSALQTALIGSV
                     AAGAAFLIAKLISE"
     gene            complement(1766..2497)
                     /locus_tag="VFV61_11190"
     CDS             complement(1766..2497)
                     /locus_tag="VFV61_11190"
                     /inference="COORDINATES: protein motif:HMM:NF014090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HEX4901088.1"
                     /translation="MEHDHSSEAIEERLAAGPGHNYLRDWIYGGVDGSVTTFAVVTGV
                     VGAQLSPWVILVMGFANLFADGFSMAASNFLGTKSEHEDLKRLEAIENRHIDQEPDGE
                     REEVRQIYLNKGFEGDDLNRVVDLVTSDRERWVKTMLTEEYGLPHQVRSPWRAAASTF
                     SAFMICGLVPLIPYLFGLGRPLLVSVVLTGSVFFIIGSIRSRWSTASWWYSGLSTLLV
                     GSAAAGLAYLIGALLNHFAPQISVG"
     gene            complement(2509..2781)
                     /locus_tag="VFV61_11195"
     CDS             complement(2509..2781)
                     /locus_tag="VFV61_11195"
                     /inference="COORDINATES: protein motif:HMM:NF013346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HEX4901089.1"
                     /translation="MDWRILLTTFGVIFLAEMGDKTQLAAMTMAAQSKRPWTVFIGAS
                     VALIAVSAIGVMVGGIVGNYVPLIWVKRVAAISFIVIGVLILLDKF"
     gene            complement(2971..>3858)
                     /locus_tag="VFV61_11200"
     CDS             complement(2971..>3858)
                     /locus_tag="VFV61_11200"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX4901090.1"
                     /translation="HLIKEIIKIMRETLPKSIEVVYEIPDDLWIISADATQVHQVLMN
                     LCVNARDALPHGGSITIKAENVFVDENYARMHLEAKPGRFVVITVADTGTGMAPEIVS
                     RIFEPFFTTKDMTKGTGLGLSTALTIVKSHGGFINVYSEPGKGTQFAVYFPSLETDEA
                     AARVLGSTDLPSGHGELILVIDDEEAIRQITASTLETFGYRVLTASDGTEAIPLFAQR
                     GNEIALVLTDMLMPFMDGPATIRALLKMNPKVKIIAASGLTAGHKPGEASLEGVRMFL
                     GKPYTAERLLTAIAQTLKS"
BASE COUNT          857 a         1087 c          998 g          916 t
ORIGIN      
        1 cgcatacatc tgctccgctg gtgggccctg gtcatcgtta ccgccatcac gctctatctt
       61 tgttggctga tgctgcaacc ttttgtgaag gtgatcagtt gggccgctgt gctggtcatc
      121 gtcttctatc ccgtgcacaa gcagatcttg cggcgcacac gcaaacccgc cctcagcgcc
      181 ctggtatcct gcgcccttgt gattcttgtc atcctcgtcc ccgttgcgct gatcactctt
      241 gccgttgtga gtgagatgtc cggagcgatg cagaacttgc agtcagcaat tacctacgta
      301 ctggatccca actcacgtat caccgggcca atactcggct ggctgggtca ctatcttgac
      361 ctctcgcgct tgcaaacaga cgaatacttg ctgacacaac ttcaaagtat gagcgggtcg
      421 attgcgggtc gcacgctggg ttttttgggt ggcttgatta gcgtggtagt acaaatggtc
      481 ttcgtaatct tcacgatgta ctacttcttc aaagacgggg aaaagattct cacagcagtg
      541 cgtgatgtcc tgccgctcga gcgtcgcgag gcagagggtg tgatggcgcg cactcgtgag
      601 gtgattgatg cgagcgtgta tggcgtgatc acgatagcaa ttatccaggg cgcattgggt
      661 ggccttgcat tctggctgct gggactgccg tcggcaatta tctggggcgt ggctatgagc
      721 tttttttcga tgattccaat attgggcgct ttcgtggttt gggtgccagc cgcgatctac
      781 ctcgcctcga cgggccattg gggcaaagcg ttaatgctgg tgctctgggg cactctcgta
      841 atcggaacga tcgacaattt tctgcgacca aagctggtgg gtagtcgtac gcgattacac
      901 gagttgctga tcttcttctc agtcttaggc gggttgcagg tgttcggtgt gctgggtgtt
      961 gtcctgggac cggtggtagt tgcaatcgcg ctcgcgctga aggacgtgtt tcaaaaggcc
     1021 agtcacgaga cggcctagaa aacgcacccg ccgaaatagt cattccgaaa tcaactttgc
     1081 gatcaaaaac gctgcacccg ccgcgacact accaatcaac gcagtctgca gcgcacttct
     1141 gagaggcctc atgccggtga atctcccttt cacgtaccca aatacgaaca acgccacgag
     1201 cgtcaccgca atcgagtaaa gcagcgcacg cggggccacc ggaataaaca ggtatggcgc
     1261 caacggaacc aagcctcccg ccacgtatgc gccgccaatt gttagtgcgc tggtcattgc
     1321 tcgctttggg ttgggccttt ccaaacccaa ttcaaagcgc atcataaagt cgatccactt
     1381 ctttggttgt cgccgcaggg cctggacaat tggttcggtc tcctctgcac tcaagccata
     1441 ttcctgaaag acttcagcga cttcgcgcat ctcctcgtgt gggatctctg ccacctcgcg
     1501 cttttctcgt tctctttcct tggcgtagtg ctcggcatca cttttggcag cgagataacc
     1561 gccgagtccc atggctattg cgccggccgc aatctcagcc agcccggctg tgacgatgat
     1621 gttggtctgt acgatggcgc ccgatagtcc cgccgccaac gcgaacggaa cagtcaagcc
     1681 atcggacatg ccgatcacga cgtcgcgcac tacttcgctg gctgtgaagt gacgttcggt
     1741 gtgcggggtt tgcggcatcc ctgctctacc cgacggaaat ctgcggagcg aaatgattca
     1801 gcagggcacc aatcaggtag gccaatcccg ctgcggcgga gccaaccagc aaggtggaga
     1861 gtcctgaata ccaccaggac gccgttgacc agcggctgcg gattgaaccg ataatgaaga
     1921 acacagagcc cgtgagcacc accgatacaa gcagtggtcg cccaagcccg aacaagtaag
     1981 ggatcaacgg cacgagtcca cagatcatga aggcgctaaa cgtgctggcc gctgccctcc
     2041 agggtgacct aacctggtgc ggcagcccat actcttccgt gagcattgtc ttgacccaac
     2101 gttcacggtc ggaagtaaca agatccacaa cccggttcag atcgtcgcct tcaaatccct
     2161 tgttcagata aatctgcctg acctcttctc gctcgccgtc gggttcctgg tctatgtgac
     2221 ggttttcaat tgcttctaag cgcttgagat cttcgtgctc agacttggta ccgagaaaat
     2281 tactggcagc cattgagaac ccatcggcaa agagattggc aaaacccatc accagaatca
     2341 cccagggcga gagttgcgca ccaaccacac ccgtcaccac ggcaaacgtg gtgaccgatc
     2401 catcaacccc gccatagatc cagtcgcgca agtagttatg gccaggaccg gctgccagtc
     2461 gttcttcgat cgcttcggat gaatggtcat gctccattgt cgctatccct aaaacttgtc
     2521 caaaagaatc aagacgccaa tcacaataaa agagattgct gccactcgtt tgacccagat
     2581 caggggaaca taattcccta cgataccacc aaccattacg ccgatggccg atactgctat
     2641 cagcgcaact gaggcgccaa tgaagacagt ccagggcctt ttggactggg cggccatcgt
     2701 cattgcagcg agctgtgtct tatcgcccat ctccgcgaga aagatcacgc caaaggtagt
     2761 gagaaggata cgccaatcca tttagtggcc acctctatcg aaaattattt ttgacgaccg
     2821 tcttacatga tcgctcttcg tcatacaagc aacaattctt gcttacagga ctgccaaaaa
     2881 aagatccacg aaattacgcg aaacagccac gcaatcgttt tcgtgctggt ctgcgttttt
     2941 cgtggatcag agtttacttt atctgccctt ctagctcttc agagtttggg caatagccgt
     3001 caaaagccgc tctgctgtat agggcttgcc gagaaacatt ctgacacctt ccagagatgc
     3061 ttccccgggt ttgtgcccag ccgtcaggcc actagccgca attatcttta cctttggatt
     3121 catctttaga agtgcgcgga tagtggctgg tccatccatg aagggcatca gcatgtctgt
     3181 tagcaccagc gcaatctcat ttcctctctg cgcaaaaagc ggaatcgctt cagtgccatc
     3241 actggcagtc aaaacccggt aaccaaaggt ctccagggtg ctcgccgtga tctgccgaat
     3301 cgcctcttcg tcgtcgataa caagtatcag ttcaccatgt cctgagggta ggtcggtact
     3361 acccaacaca cgcgctgcag cttcatcggt ttccagcgat gggaagtaca cggcaaactg
     3421 ggttcccttc cccggctcac tatagacatt gatgaatcca ccgtggctct tgacgatggt
     3481 taacgcggtg gagagaccaa gtcccgtacc tttggtcata tccttggtgg taaagaaagg
     3541 ttcgaatatc cggcttacga tctctggggc catgcccgtt ccggtatcgg cgacagtgat
     3601 tacgacaaat cggcctggtt tagcctccag gtgcatgcgc gcatagttct catcaacgaa
     3661 aacgttctct gctttgatgg taattgagcc accgtgtggc aatgcatcgc gagcgttcac
     3721 gcagaggttc attaagacct ggtgcacttg cgtagcatcg gccgagatta tccagagatc
     3781 atcgggaatc tcgtagacga cctcaatgga cttcggcaag gtctcacgca tgatcttgat
     3841 gatctcttta atcaggtg
//
LOCUS       DASUEX010000675         4721 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_557436, whole genome shotgun sequence.
ACCESSION   DASUEX010000675 DASUEX010000000
VERSION     DASUEX010000675.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4721
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_557436"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="VFV61_11205"
     CDS             <1..509
                     /locus_tag="VFV61_11205"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX4901091.1"
                     /translation="NNAGIEGKWAAITDLAEDEWDRVLNINLKGTFLCLKYEARAVLD
                     GGHGGAIVNIGSVNSFLGFQTGSAYVTSKHGLIGLTTCVSAELAPQGIRVNLVCPGFI
                     DTPMHHRGRAILGDDLYDKGVLPTVHLRRAGRPEEIAQSIVFLCSDEASYITGTTLTP
                     DGGFTLTI"
     gene            594..1052
                     /locus_tag="VFV61_11210"
     CDS             594..1052
                     /locus_tag="VFV61_11210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4901092.1"
                     /translation="MRYLAILLAALVLAACGTAPPTRSETPAASPAQAVTPEPPRASI
                     DPVQFLLTSAATDFHTHGPSGPLRFREVRTGHVMTPSGEAQYRLCGQFLRAQEGGKAE
                     WTPFATIKTSGYEQWIGADAARYCQGSSFVRDKEGDLSSSLQSRLDSLQD"
     gene            1141..1527
                     /locus_tag="VFV61_11215"
     CDS             1141..1527
                     /locus_tag="VFV61_11215"
                     /inference="COORDINATES: protein motif:HMM:NF017868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HEX4901093.1"
                     /translation="MSKHLVFIYGSLRDGCERSMSVRFPEAKFIAAATVNGSLYDLGD
                     YPGLLLSETDSPVVGEVYEVDDELLQELDDFEVTSNYLRRQVEISLGDQRQIGWTYEP
                     DPEFYSLRTLIKSGDWLEYAKTKTQW"
     gene            1796..2215
                     /locus_tag="VFV61_11220"
     CDS             1796..2215
                     /locus_tag="VFV61_11220"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX4901094.1"
                     /translation="MKAEFKTAWGYQGNNMELPVENLETAIPFYETVMGFRVLSRSES
                     PHAAAVLGRDGIQIGLAENGGDPGQDGCAFEVDDVAAAFAELKANGLEKEISEFSFEK
                     RGDTSWKVFFVVAPDGLCYWLGERQTGERAESAGGQC"
     gene            2449..2835
                     /locus_tag="VFV61_11225"
     CDS             2449..2835
                     /locus_tag="VFV61_11225"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX4901095.1"
                     /translation="MRFRSITPEDYESVRAFLSEVGWRDRVSDPEKFKKMLAHTNRTV
                     VALDGSRVVGFARALCDEVSNGYISMVAVAADKRGAGIGRELVRHLIKDDAGITWVLR
                     AGRGSSGFWERVGFQASELAMERVRT"
     gene            2919..3491
                     /locus_tag="VFV61_11230"
     CDS             2919..3491
                     /locus_tag="VFV61_11230"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX4901096.1"
                     /translation="MSNLKSTQGNGSNASIKPAYISHYGIRAQRYKEMIQWYMTVFQA
                     KVQHENEFLTFLTFDDEHHRLVIFEDTETVAKPASAAGIDHVGYGLSSFADFVLTYER
                     LKAQGIVPFLPLNHRFTTSLYYRDPDGNEVELSVDNFPTKSACDAFIRSAEMEEIGRP
                     PFGHAFDPDELAKLYHEGAPDETLARIGLP"
     gene            3590..4405
                     /locus_tag="VFV61_11235"
     CDS             3590..4405
                     /locus_tag="VFV61_11235"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX4901097.1"
                     /translation="MAERVDLFDSTYKHFTDQVLDAIRKETFGTDFGQNSWVTIDEYE
                     RLLPWLNLTAEDHVLEVASGSGGPALYLARTTGCRVIGIDANENGVATASEMAAKSGA
                     AERVQFKVANADAQLPFASDSFDGLLCIDSMNHFPNRLAVFREWQRVLRPGRRALFTD
                     PVVLTGPVTNDELASRSSVGLFLFVPPGINEQFIEQSGFKLIKQEDVTDNAALISGRW
                     FQARQAHKDDLLRIEGEERFVGLQQFFAAVQRLTSERRLSRIVYVVEKPDEVA"
     gene            4491..>4721
                     /locus_tag="VFV61_11240"
     CDS             4491..>4721
                     /locus_tag="VFV61_11240"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEX4901098.1"
                     /translation="MDEYIAGYPRDVQKLLQQVRRTIKKAAPGAAETIKYQIPTFTLN
                     GNLISFAAYKNHIGIYPAPRGVPEFAAELSAYA"
BASE COUNT         1125 a         1178 c         1333 g         1085 t
ORIGIN      
        1 tgaataacgc tggcatcgag ggtaagtggg ctgccattac tgatttggcc gaggatgaat
       61 gggaccgagt cctgaacatc aacctgaagg gcacatttct ctgcctgaag tatgaggcca
      121 gagccgtgct cgatggtgga cacggtgggg caatagtcaa catcggctct gtcaattctt
      181 tcctcggctt ccagaccggc tcggcctatg tcacctcaaa gcatggtctg attgggctga
      241 cgacctgtgt ttccgcggag ttggcgcccc agggaatcag agtaaaccta gtgtgtcctg
      301 gattcattga taccccgatg catcaccggg ggcgagcgat cttgggcgac gacttgtacg
      361 acaaaggggt gctcccaacg gttcatctgc ggcgcgcagg tcgcccggag gagatcgctc
      421 aatccatcgt cttcctgtgt tcggatgaag ccagttacat caccggcaca acgttgacgc
      481 cggatggcgg cttcacgctg acgatatgag atcgcgtgtg ccgacaaagc ctgcagcgta
      541 cgagcgatag cgtaagacgg tgacagccgg cgctaacacc caaggagaga gtcatgcgtt
      601 atcttgcaat cctgcttgcc gcattagttc tcgcagcctg cggcacggct ccaccaacgc
      661 gcagtgaaac cccggcggcg agtcccgctc aggccgttac tcctgaaccg ccgagagcgt
      721 caatagatcc agttcaattt ttacttacat cggccgctac tgattttcat acccacggcc
      781 cctcaggtcc tctccgcttt cgtgaggtgc gcactgggca cgttatgact cccagtggag
      841 aagcgcagta caggttgtgc ggccagtttc tgcgagcgca ggaaggaggc aaggctgagt
      901 ggactccttt cgcgacgatc aagacgtcgg gttacgaaca atggattggg gctgacgcgg
      961 cgcgctactg ccagggttca tccttcgttc gggacaagga gggcgatttg tcgtcatcgt
     1021 tgcagagccg tcttgattct ctgcaggatt agcctcacca ctcgtcggac gtgagagtag
     1081 tcatttacag tctccggtga acgttcgatg ttggatcgct gagaataagg gtaatgctac
     1141 atgagtaagc atctcgtttt tatttatggc agcttaaggg acggctgtga gcgctcaatg
     1201 tctgtaagat ttcccgaggc gaaattcata gctgcggcga cggtgaacgg tagcctgtat
     1261 gacctgggcg actaccctgg attattactc agtgagacgg actcgccagt agtgggcgaa
     1321 gtctatgaag ttgatgacga gctgctgcag gagttggatg attttgaggt cacaagcaac
     1381 tacttgcgca ggcaggtgga aatctctctc ggcgaccaaa ggcagatcgg ttggacttat
     1441 gaacctgatc ccgaattcta ttcacttcgc acgttaatca aatcgggtga ttggctcgaa
     1501 tatgcgaaga cgaaaaccca atggtgaagt cagcttctaa ccatgatttc gctgccaata
     1561 gaatcggagt tattccccat gacttctaca agccgattcg acgcaaacac gaaactcgtt
     1621 ggaaattacc tcgccgcgaa ggagaaagca gcgggccagg cagcacaact cgttcaaccc
     1681 tgaagccttg atagcagctc gatcttgacg tcgaattcac acagtatgaa acctcgactc
     1741 ttttgttgtg cggaaagcgc gagtcctttc gcaacttatt ttcaaggagg ctgaaatgaa
     1801 agcagagttc aagactgcct ggggatacca gggaaataat atggaactcc cggtggaaaa
     1861 cctggaaacc gcgattccct tttacgaaac cgtgatgggt tttcgcgtat tgtcgcgcag
     1921 cgagtcgcca catgcggcag cggtgcttgg cagagatggc atccagatag ggttggctga
     1981 gaacgggggc gatcctggcc aggacggctg cgcttttgag gtcgacgatg tcgcggcggc
     2041 cttcgccgag ctaaaagcta acggcctgga gaaagaaata tccgaattca gcttcgaaaa
     2101 acgaggcgat acgagttgga aagtattctt cgtggttgcg ccagatggtc tctgttactg
     2161 gttgggtgaa cggcaaaccg gcgagcgagc ggagagtgcc ggcggccaat gctgaaggca
     2221 aatgaaaggc gcgtactcaa tgaatcaagg tcttgccaaa agtctcaatt gaagaagtcg
     2281 acgcatagcg tcgaacgatt ttagcccggt ctttcaaggc cgggtgtagt cgcaggtaat
     2341 gaattctgcg tcgcgtagcg acgaatgaat cgcaaatcca cagcgtcgta cttattgcag
     2401 ttactttaag gtaaagccac gaattaagct acatttcggt tcaaagaaat gagattcaga
     2461 tcaataactc cagaagacta tgaatcggtg cgcgcgttct taagtgaagt ggggtggcgc
     2521 gatcgcgttt ctgatcccga gaagttcaag aagatgttgg cgcacaccaa ccggacagtg
     2581 gtggcacttg atggttctcg cgttgtcgga tttgcgagag ctctatgtga tgaggtctct
     2641 aatggttata tctctatggt tgcggtagct gccgataagc gtggagcggg catcggtcga
     2701 gagctcgtgc gacatctgat caaggatgac gccggaatca catgggtttt gcgagcaggt
     2761 cgcggcagca gcggattctg ggagagagtt ggtttccagg cgtcggagct cgcgatggag
     2821 agagttcgga cttgaagctg cgcacttgaa agatcaagtc agtggaaaga tcataagcat
     2881 cagcacgtag cttctttgaa ccaatcagga aaatgattat gagcaaccta aaatcaacac
     2941 aaggtaacgg atcgaacgcg agtattaagc ctgcttatat ctcgcactac ggtatccgcg
     3001 cgcagcgata caaagagatg atccaatggt acatgacggt cttccaggcc aaggtgcagc
     3061 atgagaacga attcctcact ttcctgacct ttgacgacga gcaccaccgg ctggtgatat
     3121 tcgaggacac cgaaactgtg gctaagccgg caagcgcggc gggcatcgat cacgtcggtt
     3181 acggcttgtc tagcttcgcc gattttgttt tgacttacga gcgtcttaag gctcagggaa
     3241 tcgtgccttt cctgccgttg aaccacaggt tcacaacttc gctttattat cgcgatcccg
     3301 acggcaatga ggtagagcta agcgtcgaca acttccccac gaagagcgct tgtgacgcct
     3361 tcattagaag cgcagagatg gaggagatcg gcaggccgcc tttcggccat gccttcgacc
     3421 ctgatgaact cgcgaagctc tatcatgaag gcgctcccga tgagacgcta gcgcggattg
     3481 gcctgccgta acgcgttagt aatagtcttg ctttcatctc ttcaacttct tggcgaagct
     3541 cgcgatactt aacgacgttg agaatttcct ttagtaagga tttcgtttca tggctgaacg
     3601 cgtcgatctc ttcgacagta cttacaagca cttcaccgat caggtgctgg acgcaatccg
     3661 caaggaaacg ttcggcaccg atttcggaca gaacagttgg gtgaccattg atgagtatga
     3721 gcggctgctc ccctggctga accttacggc tgaggatcac gtgctggaag tggcgagcgg
     3781 ctcaggcggt cctgctctct accttgctcg cacaacgggt tgtcgcgtca tcggaatcga
     3841 cgccaacgaa aatggcgtgg cgaccgcttc agaaatggca gcgaagtctg gggccgccga
     3901 gcgcgttcag ttcaaagttg ccaacgccga cgctcaattg cctttcgcaa gtgactcctt
     3961 cgatgggctg ctctgtatcg actcgatgaa ccattttccg aaccgcctcg cagtgttccg
     4021 tgaatggcag cgcgtcctgc ggccgggccg gcgagcgctt ttcaccgatc cggtcgttct
     4081 tacgggtcca gtaacaaatg atgaattagc gagtcgcagt tccgtcggtt tgtttctctt
     4141 tgtgccgccg ggaatcaacg agcagttcat cgaacaatcg ggctttaaac taatcaaaca
     4201 agaagatgtg acagacaacg ccgctttgat ctctggccgc tggttccagg cacgacaggc
     4261 tcataaggac gacttactgc gtatcgaggg cgaagaacgc tttgtcggcc tgcaacaatt
     4321 cttcgcggca gtgcagcgcc taacaagcga acgaagactc tcaagaattg tttatgtggt
     4381 ggagaagcca gacgaagtag cttaactgct agtttgccag gccaaccccc tcctgattgc
     4441 gctgtgggat cgttctaatc atgaatggtg gtagaagaaa tcctaaagat atggacgaat
     4501 acattgcggg ctatcctcgc gatgtgcaga agctattgca acaggtcaga agaacgatca
     4561 aaaaagcggc accgggcgcc gccgagacga tcaagtatca aatcccgacc tttacgctca
     4621 acggaaacct gatttctttt gcggcctata aaaatcatat tggtatctat cccgcgccca
     4681 gaggcgttcc ggagtttgcg gctgagctat ctgcttatgc a
//
LOCUS       DASUEX010000676         2447 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_558364, whole genome shotgun sequence.
ACCESSION   DASUEX010000676 DASUEX010000000
VERSION     DASUEX010000676.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_558364"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..565
                     /locus_tag="VFV61_11245"
     CDS             <1..565
                     /locus_tag="VFV61_11245"
                     /inference="COORDINATES: protein motif:HMM:NF024822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4112 domain-containing protein"
                     /protein_id="HEX4901099.1"
                     /translation="ADGIRSRKTGEIMQAFKEFERETLQPARIKHPAKVQESIETLSW
                     LMDDVFRVPLVGWRFGLDAVIGLVPGLGDTATSLASFYILASAVRYRVPKITLLRMGM
                     NIGIDYVLGSLPLAGDFFDAWWKSNQKNVALLRERATVSAEEVREGRLSDWFFVGLII
                     LILTGLAVGAAFVSVSLLLLVLSQFSN"
     gene            complement(562..1038)
                     /locus_tag="VFV61_11250"
     CDS             complement(562..1038)
                     /locus_tag="VFV61_11250"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEX4901100.1"
                     /translation="MRIGTEMPALAGATVWFGSTQAQAEAEAKGRPTLVHFWSVSCGT
                     CKENLPRLAQWREEKKEDGLRVIAVHMPRYEADTDLEAVRAAIATYEITEPCAVDNEH
                     QLKDAFQNEQGYVPAYYLFDAEGKLRSFAAGERGLDMLKAALDRVLAQTAVTASQG"
     gene            complement(1113..1685)
                     /locus_tag="VFV61_11255"
     CDS             complement(1113..1685)
                     /locus_tag="VFV61_11255"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019258276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX4901101.1"
                     /translation="MTAAGTALAVDKAMVGQPAPDFDMASTKNLEKLNENVKLADYKG
                     KWVVLLFYPLDFTFVCPTELTMFSDRYEDFQGIGADVIGVSTDSVFSHRAWIKTPRDK
                     GGVEGLKYPLASDATKNVARDYGVLLEDRGIALRGLFVIDPEGILRYKVVHDLNIGRS
                     AEETLRVIQALQTGGLCQAEWKPGQKTLNA"
     gene            complement(1752..2165)
                     /locus_tag="VFV61_11260"
     CDS             complement(1752..2165)
                     /locus_tag="VFV61_11260"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEX4901102.1"
                     /translation="MKKAAKAGGGQKLTPQREAVLAVIREREDHPTANDIFEAARKQL
                     PSLSYATVYNSLKFLREAGLVREISFGGGANRYDREICRHDHAICSQCGKLVDFDLPE
                     AAELLRAAARKSRFTPESVHLTLTGICPECRKAGS"
BASE COUNT          553 a          677 c          628 g          589 t
ORIGIN      
        1 agcggatgga atacgtagca gaaaaaccgg agagattatg caagctttca aggaattcga
       61 acgggagact cttcagccgg ccaggataaa acacccggct aaggtacaag agagcattga
      121 gacgctgagt tggctgatgg acgacgtatt ccgcgtgccg ctggttggct ggcggtttgg
      181 actggacgct gtaatcggat tggttcccgg cttgggggac acagcaacat cgctcgcgtc
      241 tttctacatc ctggcctccg cagttcgtta ccgtgttccg aaaattaccc tgctgcggat
      301 gggaatgaat atcggcatcg actacgtgct tggctcactt cctttagcag gagacttctt
      361 tgacgcctgg tggaaatcca atcagaagaa tgttgcactg ctgcgtgaac gagcgaccgt
      421 ctcggctgaa gaggtccgcg aaggcaggct tagcgattgg ttttttgtgg gattgataat
      481 tctgatattg accggtttag cagtgggagc agcgtttgtg agtgtgtcgt tgctgctcct
      541 cgtcctcagt caattctcaa attaaccttg ggacgcagtc acggccgttt gagcgagcac
      601 tcgatccagc gcggctttca gcatgtccag ccctcgttca ccggctgcaa aacttcttaa
      661 ctttccttcc gcgtcaaaaa gataataggc aggaacgtaa ccctgctcat tttgaaatgc
      721 atccttaagt tgatgctcat tgtctacggc gcaaggttct gtgatctcat aggtggcaat
      781 tgctgcgcgg actgcctcca gatcggtatc ggcttcatag cgcggcatat ggacagcaat
      841 aactcttagg ccatcctctt tcttctcttc tcgccattgg gccagccgcg gcaggttttc
      901 cttgcaagtg ccacaactga ctgaccagaa gtggacgagg gttggacgcc cttttgcttc
      961 agcctcggct tgagcctgcg ttgacccaaa ccacacagtg gctccagcaa gcgccggcat
     1021 ctcagttcct attcgcattg gcatactctt caactccctg aaaaactacg ggtgccaggt
     1081 ttgagcgacc tgacaccctg gctcgaatcc cattacgcat taagcgtctt ttgacccggt
     1141 ttccattcag cctggcaaag cccaccggtt tgcagtgcct gaatcacacg caacgtctct
     1201 tccgccgagc ggccgatgtt gagatcgtgc accaccttat accgaaggat accttcggga
     1261 tcgatgacga atagtccgcg cagcgcaatc ccgcgatctt caagcaacac accgtagtcg
     1321 cgcgccacat ttttggtggc gtccgaggcg agcggatact tgagtccttc gactccgcct
     1381 ttgtctcgcg gcgtcttgat ccacgcgcga tgcgaaaaga cggaatcagt ggaaactccg
     1441 atcacatctg ctccaatccc ctgaaaatct tcgtaccgat cgctgaacat cgtcagctct
     1501 gtcggacaga cgaaagtgaa gtcgagggga taaaagagaa gaaccaccca tttccctttg
     1561 taatcagcaa gctttacatt ctcgttcaac ttttcgaggt tcttcgttga ggccatatca
     1621 aaatcaggcg ccggctggcc caccatcgcc ttgtctaccg ctaaagctgt tcctgctgct
     1681 gtcatgaatc ctccaattct caatttaaag atcgttttcg actcacaccc attggtgtga
     1741 ggcatatcac cttaagaccc tgccttgcga cactccggac agatacccgt aagcgtcaag
     1801 tgaaccgact caggtgtgaa acgcgatttg cgcgcagctg cgcgcaacag ctctgcggcc
     1861 tctggcaagt caaagtcgac aagcttgcca cactggctgc agatcgcatg gtcatgtcta
     1921 cagatctcgc ggtcgtaacg gttcgcgcca cctccaaacg agatctcgcg caccagtccg
     1981 gcctcgcgaa gaaacttaag agagttgtag acggttgcat agctcagcga cggtaactgc
     2041 tttcgcgccg cttcaaaaat atcattcgca gtgggatggt cctcccgctc gcgaattaca
     2101 gccagcaccg cctcccgctg gggagtcaac ttctggccac cgcctgcctt tgctgctttc
     2161 ttcatgatcg ccaaactgga atggatttag actaattcta aattgatcgc ttgtcaaccg
     2221 gccgtgaagg ccttgcacaa aatcaggcag agtcgcgtgg ggttgtcgcc ttcaatccat
     2281 cacttcagga caactgcgga tggcttgcgg cgaaatcgac aatcgccttc acataacttt
     2341 tgaatggggg gcaatggatt gcgtgggcgt tgagtaaggc gcccgattcg gttgtgtcat
     2401 acgtctgacg caaaaaaaag tagggcacac cgtgatgggg caaacca
//
LOCUS       DASUEX010000677         2121 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_558688, whole genome shotgun sequence.
ACCESSION   DASUEX010000677 DASUEX010000000
VERSION     DASUEX010000677.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_558688"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1172
                     /locus_tag="VFV61_11265"
     CDS             <1..1172
                     /locus_tag="VFV61_11265"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX4901103.1"
                     /translation="YTAGQRVIGRGVKVGDVKTESPLYVAGVRPGDTLLEVENQRIGN
                     PEELVNALAASDRHPARVKAFRYDWPLVFKVEKGKLLAGNTDLDELGIQSWSRDRSWR
                     ASGFYGHYVTYAEVLQLILALALALFISLPSKRTWSGVLLLVAFAGFGFALLLTMTRA
                     AWLAFLISSTVIILLGAGRRGVIVVGALAIPLILAGLFILQQKRNVGFFDSSEGSTTW
                     RITVWREGVNLLTSKPRHLLFGIGMDSLKGHWREWGMFDHGRLPIGHMHSNLLQIALE
                     RGIPALLVWLLLLGAYARMLWQLSRKLGTGATDEAAGASGSLKLGTWVDRGLTLGALG
                     GLVGFFVSGLVHYNWGDSEVVMIFYFIMGLALVLNRMVADGQSFGRRASNSHKAS"
     gene            complement(1150..>2121)
                     /locus_tag="VFV61_11270"
     CDS             complement(1150..>2121)
                     /locus_tag="VFV61_11270"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX4901104.1"
                     /translation="RGERLRTFTVDLSSFYFAGLLWFKLLARLIAFRPHIVYIASCYD
                     WSYLRDAVLMLTARLFGAKVVCHFHGRRSGPLFTAPSGIMRWLLRLTTYSFNKIIFLS
                     EGLKESLLPIFGNGKAEVVSNFVDVRDFRAAELSDNEEARIVFIGRLSDDKGIFELLE
                     AASLLRREGRKLMVDILGVAETDAEECVVQTYATQAGLGAVASFHGIKTGAEKARLLN
                     AATVFVLPSKLEVFPLSLLEAYASGLPAVCVRVGAVPEILKEGENGFLVPPGNTQALV
                     DSLRRILDDTQLRQVMGAKNRSAAESLYSKDQAVRSLSHIFNSLYAS"
BASE COUNT          472 a          550 c          591 g          508 t
ORIGIN      
        1 tctataccgc aggtcagagg gtgatcggcc ggggcgtaaa ggttggagat gtaaaaactg
       61 agagtccttt gtacgtcgca ggggtgaggc ccggcgatac cttgcttgaa gttgaaaatc
      121 aacggattgg caatccagag gagctggtta atgcccttgc cgcctctgat cggcatccgg
      181 caagggtcaa ggcatttcgc tatgactggc cgctggtctt caaagtggag aaaggaaaac
      241 ttctcgccgg caatacggat ttggatgaac tcggaatcca atcgtggtcc agggatcgca
      301 gctggcgagc tagcggcttc tatggccatt acgttaccta tgcggaagta ttgcagctaa
      361 tcctggccct cgccttggcc ctctttatta gtctacccag caagcgcacc tggtcgggtg
      421 tgttgttact cgtggcgttt gcggggtttg gctttgcgct attgctaacc atgacgcgtg
      481 ccgcctggtt ggcgttcctc atctcttcaa cggtgatcat tctgctgggt gcgggccgcc
      541 gtggtgtcat cgtcgtcggc gccctcgcga tccccttaat tttggctggg ctctttattc
      601 tccagcagaa aagaaatgtt ggcttctttg attcgagcga gggctcgacc acttggcgga
      661 taacggtatg gcgcgaagga gttaatctcc tcaccagcaa gccacgtcat ctgctttttg
      721 gcattgggat ggattcgcta aagggccatt ggcgtgagtg gggcatgttt gatcacggac
      781 ggcttccgat tggacacatg cattcgaatc tgctgcaaat tgcgcttgag cgaggaatac
      841 ctgctctgct ggtttggctg ttgttgctcg gcgcctacgc gcggatgctc tggcagttaa
      901 gtcgcaaact aggaactggc gccacggatg aggcagccgg cgcatctgga tcgctcaagc
      961 tcggaacgtg ggtagaccgt ggcctcacgt taggggccct tgggggactg gttggtttct
     1021 tcgtcagcgg cttggtgcac tacaactggg gcgactcgga ggtagtaatg atcttctatt
     1081 tcatcatggg gttggcgctg gttttaaacc gcatggtggc cgatgggcaa tcttttggtc
     1141 gtagggcttc taactcgcat aaagcgagtt gaaaatgtgc gataagctcc tgactgcctg
     1201 gtccttgcta taaagtgatt ccgccgccga acggttcttt gctcccatca cttgtcgcag
     1261 ttgtgtgtcg tccaggattc tccggagaga atccaccaat gcttgcgtgt ttcccggagg
     1321 caccagaaac ccgttctcac cttccttgag aatttccgga acggcaccca cacgaacaca
     1381 gactgccggc agcccgctgg cgtaagcctc cagtagggaa agcggaaaga cttcgagttt
     1441 tgaaggcagt acaaagacag ttgccgcatt caacaacctc gctttctccg cgccggtctt
     1501 gattccgtga aaactagcga cagctccgag tcccgcctgg gtggcatagg tctgaacaac
     1561 gcattcctca gcatcagtct cagcgacgcc cagaatatct accatcagtt tccgaccttc
     1621 gcgccttaag agcgacgctg cttccaataa ctcaaaaatc cctttgtcat cgctcaaacg
     1681 tccgatgaag acgatcctgg cttcctcatt atctgaaagc tcggctgcac gaaaatcacg
     1741 gacatcaacg aagttcgaca ccacttccgc tttgccgtta ccaaaaattg gcagcagcga
     1801 ctctttcaaa ccctcagaca ggaagatgat cttgttgaat gagtaggtcg tcaatcgtaa
     1861 taaccaacgc atgatgccgg aaggagcagt gaagagtggc ccactgcgac gtccatgaaa
     1921 atggcagacc accttggcgc caaaaagtct ggcggtcagc atcaagacag cgtctcttaa
     1981 atagctccag tcgtagcagg aagcgatgta gacgatgtgt gggcgaaaag ctatcagtct
     2041 ggcaaggagt ttgaaccaaa gcagaccagc aaaatagaag cttgagagat cgacggtgaa
     2101 tgtccgcagc cgttcaccac g
//
LOCUS       DASUEX010000678         3435 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_684169, whole genome shotgun sequence.
ACCESSION   DASUEX010000678 DASUEX010000000
VERSION     DASUEX010000678.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3435
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_684169"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..568)
                     /locus_tag="VFV61_11275"
     CDS             complement(<1..568)
                     /locus_tag="VFV61_11275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4901105.1"
                     /translation="MNAFITRSTISTGVAAALLIFAGCAANPENANTTATNVVPPPPI
                     QKLTLVARPQAIMDLMKQRGEQDEAQPTLKIVTPAKDAVVDGANVDVKLDLSGDLRGY
                     MPHKDPATGKGNHIHVILDNEPYEAYYELNQPFQLRNLMAGKHTLRVFASRPWHESYK
                     NDSAFQMVSFTVKGGGDASQPTTTNSGQT"
     gene            894..1937
                     /locus_tag="VFV61_11280"
     CDS             894..1937
                     /locus_tag="VFV61_11280"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HEX4901106.1"
                     /translation="MNTKTSRLALVTISGLLITTALLVGCNRTNQTAEFQPEILPLPP
                     QLTTYEAMPIPADNPLTPEKVALGRQLFFDERLSGDGSRSCYSCHVCEKGLTDGLAKA
                     VGAFNKQLPRSSPTLWNIGYHKEFYWDGRSGSLEKQAMAAWTGANMGAKADEVAAKLN
                     AIPGDRSQFHGVFGGDATPDNIVKAIAAFERTIISGDTAFDRYRAGDTGAMSEAAVRG
                     WNNFQAIKCTNCHDGVLFTDQQYHNVGIGMDQKEPDVGRFKVTNKPEDTGAFKTPTLR
                     DIAKSAPYFHDGSAQTLEAAVDIMLGGGKENEHLDKKNLQKHDLSPDMRESLLEFLKA
                     LNVDCKLTKPRLP"
     gene            complement(2051..3094)
                     /locus_tag="VFV61_11285"
     CDS             complement(2051..3094)
                     /locus_tag="VFV61_11285"
                     /inference="COORDINATES: protein motif:HMM:NF013384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low specificity L-threonine aldolase"
                     /protein_id="HEX4901107.1"
                     /translation="MPNQAKRSRSFASDNNAGVHPEILEAIIRANEGHVVAYGDDPYT
                     DHAIKVFKKHFGKQVEVFFVFGGTGANVLGLKAATESYHAVVCAETAHINVDECGAPE
                     KFTGCKLLTVPTPDGKVRTEQVKPFLEHIGFEHHVQARVISISQATEMGTVYTASELK
                     ALSSFAHQHEMLLHMDGARIANAAVSLSTDFKKITSDVGVDVLSFGGAKNGMMFGEAV
                     VFFDPKLAAGFKYLRKQGTHLPSKMRFISAQFTALLEKDLWRRNAEHSNRMARVLAAE
                     LAQIREIKITQKVQSNGVFAILPKRYVPILQKKYFFYVWNEATSEVRFMTSFDTTEDD
                     IRDFVGLVKRTVT"
BASE COUNT          820 a          904 c          881 g          830 t
ORIGIN      
        1 tggtctgacc ggagttggta gtcgttggct ggctggcgtc gccaccgcct ttaacggtga
       61 agctcaccat ctgaaaagca gaatcattct tgtagctttc atgccacggt cgtgaagcga
      121 agacacgcag agtgtgtttg cctgccatca ggtttcgcaa ctggaacggc tggttgagtt
      181 cgtaatacgc ctcgtaaggt tcgttatcaa gaatcacatg aatgtggttg cctttacccg
      241 tcgccgggtc tttgtgcggc atataaccgc gcaagtcccc gctcaaatcg agcttgacgt
      301 ctacattcgc gccatccaca accgcatcct tagcgggcgt aacgattttc aatgttggtt
      361 gcgcttcatc ctgttcgccg cgctgtttca tcaagtccat gatcgcttga ggacgcgcaa
      421 cgagcgtgag tttctggatg ggtggtggcg gaacgacgtt ggtcgcggtc gtattcgcgt
      481 tctcaggatt agccgcacaa ccagcaaata tcagaagtgc tgcggccacc ccggtggaaa
      541 tagtgctgcg agtaatgaaa gcattcatat caattcctcc cgatgattag tcttcgtcgt
      601 tctagtgtga acttcgagta tggcaaatat tgaacgagaa tgcgtcgaat aggtttcacc
      661 gtctcctcaa gtctcagggc tcaggtctca ggtctcaaaa tggctcagat gagatccaca
      721 tctggacatc gaacgcaaaa ctgaaccttg agacctgaga cctgagacct gagacttgag
      781 actttttaga gtcgagactt gagacttaga ctggtgtaga atccgcgcac acggagtaac
      841 taccaatcat tggaggaaag cgctggccca tccgagcgca tgtctggcga caaatgaata
      901 ccaaaacatc tcgtctggct ctggtaacta tttcgggttt gctgatcact actgctcttc
      961 tggtcggctg taatcgcact aatcaaacag ccgagtttca accggagatt ctgccgctgc
     1021 caccgcagct aacaacatac gaggcgatgc cgattcccgc tgacaatccg ctgactccgg
     1081 aaaaagtcgc tttgggacgg cagttgtttt ttgacgagcg tttgagtggg gacggctcgc
     1141 gctcgtgcta ctcctgtcat gtctgcgaga agggtttgac ggatggactg gcgaaagccg
     1201 tcggcgcttt caataaacaa ttaccgcgca gcagtcccac gctctggaac attggttatc
     1261 acaaggaatt ttattgggac ggtcgcagcg gttcgttgga gaaacaggca atggcggcct
     1321 ggacgggcgc caacatgggc gctaaggctg atgaagtcgc cgccaaactt aacgcaatcc
     1381 ccggcgaccg ctctcagttt cacggtgtct ttggcggcga cgccacgcct gacaacatcg
     1441 tcaaagcgat tgcggccttt gaacgaacca tcatcagcgg cgacacagct tttgatcgct
     1501 atcgtgcggg cgataccgga gcgatgagtg aagctgcggt gcgcggctgg aataatttcc
     1561 aggcaatcaa gtgtaccaac tgccacgatg gcgtgctgtt tactgatcag cagtatcaca
     1621 atgtgggcat cgggatggat cagaaggagc cggatgtggg acgtttcaaa gttacgaaca
     1681 aacctgaaga caccggtgcc ttcaaaacgc caacactccg cgacatcgcg aagtcagcgc
     1741 cctactttca cgatggcagt gcgcaaactc tggaagctgc tgtagacatc atgctcggcg
     1801 gaggaaaaga gaacgagcat ttggataaga agaacctgca gaagcacgac ctctctccgg
     1861 acatgcgcga gtcgctgctg gaattcctga aggcgctgaa tgttgattgc aaactaacca
     1921 aaccgaggtt gccttgaacc gtccaaagtc aaaatgtcca aggtccaacg tccgctaagc
     1981 aaactattgc attacttata caagatcact ccggccctca gtggcgacct tgaaccttgg
     2041 actttggact tcaggtaacc gttctcttta ctaaacccac aaaatccctg atgtcgtctt
     2101 cagtggtatc aaacgaagtc ataaacctca cttctgaagt cgcctcattc cacacgtaaa
     2161 agaagtactt cttttgcagg attggcacgt atcgcttcgg caaaatcgcg aacacaccat
     2221 tggattgcac cttctgcgtg attttgatct ctcgaatctg agcgagttcc gcagccagca
     2281 cccgcgccat tcggttggag tgttcggcat ttcgccgcca gagatctttc tcgagcagcg
     2341 cggtaaattg cgccgagata aaccgcatct tcgaaggcaa gtgcgttccc tgcttgcgca
     2401 ggtacttaaa tcctgcggca agcttggggt caaaaaacac tactgcctca ccaaacatca
     2461 tgccgttttt cgcgccgccg aaggaaagga catctacacc aacgtcgctc gtgattttct
     2521 taaagtcggt gctcaagctg acagctgcat tggcaatccg cgccccatcc atgtgcaata
     2581 acatttcatg ctggtgagca aaacttgaca gcgccttgag ttcgctggcg gtatagacag
     2641 tccccatttc ggttgcctgc gaaatcgaga tcacacgggc ctggacgtga tgttcaaagc
     2701 caatgtgctc taagaacggc ttgacttgct ccgtccttac tttgccatca ggagttggca
     2761 ctgtaagaag cttgcagccg gtaaactttt ctggggcgcc acactcatcc acgttgatgt
     2821 gggccgtctc cgcgcacacc actgcatgat aagactcggt cgccgccttg aggccaagaa
     2881 cattggcgcc ggtgccgccg aagacaaaga acacctcgac ctgtttgcca aagtgctttt
     2941 tgaaaacctt tatcgcgtgg tcggtataag gatcatcgcc ataggcgaca acatgaccct
     3001 cgttagcgcg gataatcgcc tcgagaattt ccgggtgcac tcccgcgttg ttgtcactgg
     3061 cgaagctacg tgatcgttta gcttgattgg gcatgacaaa ttgaattcag gtttggttag
     3121 ttcgtttttg agctttgtac tttgagcttt gtacttggtt gttcgtgacg ctttgtaact
     3181 tgatattctc aagttaggat ccaccaagcc cggcggatta ccgaaccaag tacaaagtac
     3241 caagctcaaa gtgcaaagct cacttcttgc gcgatttcaa atccttaatt atttcccgcg
     3301 ctgcatttgc gccgcactgg ccggtaatct caccgccggg atgcgtgccc gcgccgcaga
     3361 gatacagtcc tttaattggc gtccgatatt gcgcccaacc aattattggt cggaacatga
     3421 aaaactgatc aaggg
//
LOCUS       DASUEX010000679         3524 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_684764, whole genome shotgun sequence.
ACCESSION   DASUEX010000679 DASUEX010000000
VERSION     DASUEX010000679.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3524
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_684764"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="VFV61_11290"
     CDS             complement(<1..463)
                     /locus_tag="VFV61_11290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4901108.1"
                     /translation="MSTSTLETRPEVRQQRPWSLWLRQIAAIFRLEVTKNFLGRRSIL
                     LYLLALLPIVPLALLAPFTPPGNEWQDFTQYSMIFAVYYGGLILRTVVFFGCAWIFMN
                     LFRGDIVDRSLHFYFLSPVRREVLVIGKYLSGLVTSIVLFSATTILCMLLLY"
     gene            complement(531..1472)
                     /locus_tag="VFV61_11295"
     CDS             complement(531..1472)
                     /locus_tag="VFV61_11295"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4901109.1"
                     /translation="MSESSTQIVFDDVSKFYGEILGVNRVNLSIGAGITSLVGPNGAG
                     KSTLMNLMTGLLRPTRGSITVLGTPTDQPEQLFSKVGYCTQFDSFPRGLTGREFIRAF
                     LLVHGFDKNKADAATVNALEQVNLLDAADRKVAAYSKGMRQRIRLAQAIAHRPSVLIL
                     DEPLNGLDPMVRAETIALFRKLAGEGLHLIISSHILHEVDMMSDRVVLVNNGYIVAEG
                     NIHGVRDEVKEHPMQILIRCDQPAKLAAQVFAEDSVVEARLHSDRGGLFVRTRDADSF
                     YLLLNRVVAEGEINVESVAPVDDDLSAVYGYLIGEGG"
     gene            complement(1594..2505)
                     /locus_tag="VFV61_11300"
     CDS             complement(1594..2505)
                     /locus_tag="VFV61_11300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4901110.1"
                     /translation="MAVYDHSYKNYAGALTPEWSRFLIIPRHAFRDVFKSKLFTAFFA
                     ICFLPLLVEAILIYLHHNVNALAIMRINVRELIPIDASFFQTFVNLQGGFAFFVTLLV
                     GPPLVSRDLRNNALPLYLCRPFSRTEYVAGKMSVVLILLSSMTWVPQLLLFLFQSYLE
                     GGRWFIDNLWIASAIFIASVVWILLLALLSQAISALVKWRVIASGALLGLFFIPSVFG
                     EVVNVIFRTRWGSLISLGALTRNISAGLFGTFDSTSMSVTDINGEVIRVVLSEPPLWA
                     SWAALFFVCAICLAILSAKVKAYEVVS"
     gene            complement(2492..3472)
                     /locus_tag="VFV61_11305"
     CDS             complement(2492..3472)
                     /locus_tag="VFV61_11305"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4901111.1"
                     /translation="MATAAISDLTKLDDPRNFAPVIDLNDLSVAFGKRQILQGLRGEL
                     RGRAIGLLGPNGAGKTTLIHTLLGFHPPSSGTAHIFGKDILTDAKQIRGLIGYMPERD
                     SFIAKMSAVHLVRLMAELSGLPPEAALERAHEALFYVGLGEARYRSLDTYSLGMKQLA
                     KLAQAIAHGPKLIFLDEPTNGLDPPARARMIRLIREIRDSGQANIVLSSHLLRDVEEC
                     CEEIVILKDGRLVVYCNLEEERKSNRKFLVLETRGDQARFAKAIGDLGCEYALTGDHR
                     LKVVLQETVEVRDLYRIAAEQEMQIRRLSYKRDSLEDIFLKAMENGHGSL"
BASE COUNT          913 a          986 c          897 g          728 t
ORIGIN      
        1 agtagagcag caacatgcaa agaatggtgg ttgcggaaaa gagcactatt gaggtcacca
       61 gacccgacaa atacttgcct attaccaaga cttcgcgccg caccggactg agaaaataga
      121 agtgcaaact gcgatcgaca atgtcgccgc ggaagagatt cataaagatc caggcgcatc
      181 cgaagaacac aactgttcgt aaaatcagtc cgccataata gactgcgaag atcatgctgt
      241 actgcgtgaa gtcctgccac tcatttcccg gaggcgtgaa cggcgcgagc aacgctaaag
      301 gaacgatcgg gagcaaggcc agcaggtaaa gcagaatcga gcgccggccg agaaagttct
      361 ttgttacttc caaccggaag atggcggcga tctgacgcag ccaaagcgac caggggcgct
      421 gctgtctgac ttccggtcta gtttctaaag ttgaagtgct catgatttgg agtgcggcgc
      481 cttgtcgccg cttcctattt ctgaaaaccg gtgaaacggt ttcattaaaa tcagccgcct
      541 tcgccgatca aatagccata cactgcgctc agatcatcat cgactggcgc aactgactca
      601 acattgatct ccccttcagc aaccactcga ttcaaaagca gataaaaact gtcagcgtcg
      661 cgagtgcgga caaagagtcc gccgcgatcg ctatgcagtc gcgcctccac cacactgtcc
      721 tccgcaaata cttgcgctgc gagcttcgcc ggctgatcgc agcgaatcag gatttgcatt
      781 ggatgttcct tgacctcgtc gcgcacaccg tgaatattgc cttcggcaac aatgtaacca
      841 ttgttcacaa gcaccacgcg gtccgacatc atgtcgactt cgtgcaggat gtggctggaa
      901 ataatcaagt gcagcccttc gccggcgagt ttgcgaaaaa gagctatggt ctcggcgcgc
      961 accatcgggt ctaatccgtt gagtggctcg tcaagaatca agacggacgg acgatgagca
     1021 attgcctgcg cgagccgaat tcgctggcgc atgcctttgc tgtaggccgc cacttttcga
     1081 tctgccgcat ccagcaagtt tacttgctcg agcgcgttca ccgtggcagc gtcagctttg
     1141 ttcttatcga agccgtgcac cagcaaaaac gctcggatga attcccgtcc cgtgagtccg
     1201 cgcggaaaag aatcgaactg cgtgcagtat ccgactttgg aaaagagctg ctcgggctgg
     1261 tcggtgggcg tccctaagac ggtgatactg ccgcgtgtcg gtcgcaacag gcctgtcatg
     1321 agattcatca aggtggactt gccggcgcca ttaggaccca cgagactggt aatacctgca
     1381 ccaatactca agttcacacg attcactccg agtatctcgc catagaactt ggagacatcg
     1441 tcgaagacta tttgtgttga agactcactc atcactattg ccaaatgccg attggcgatt
     1501 gccaattaaa tccagccaca aagctttcca tcgtccctta gattcacgat tgccgcacgg
     1561 ttaacaattg gcatttggca atcgccaatc gtctcagcta acgacctcgt aagccttcac
     1621 ttttgccgac aatatcgcca ggcaaatagc gcagacgaaa aacagcgcag cccaggacgc
     1681 ccagagcggc ggttcactca gcactacgcg gatcacctcg ccgttaatgt cagtaaccga
     1741 catcgaggtg ctgtcaaagg tgccaaacag tcccgcggag atgtttcgcg tcaaggcacc
     1801 cagactaatc aggctgcccc agcgcgtgcg gaagatcaca ttaactactt caccgaaaac
     1861 ggaggggatg aagaacaggc ccagcaacgc tccgcttgca atcacccgcc acttcaccag
     1921 cgccgaaatt gcctgcgaca gcaaagccag cagcagtatc caaacgacac tcgcaatgaa
     1981 gatggcgctg gcaatccaca gattatcgat aaaccagcga ccgccttcga gatacgactg
     2041 aaacaagaac agcaaaagct gcggcaccca ggtcattgac gacaacagaa tcaagactac
     2101 cgacatcttg ccggcaacat attcggttcg cgaaaacggc cggcagagat agagcggcaa
     2161 tgcgttgttt ctcagatcac gcgacacgag cggcggccct accagcaacg tcacaaagaa
     2221 agcgaagcca ccctgcaaat tcacaaacgt ttgaaagaat gaggcgtcaa taggaatcag
     2281 ctcgcgcacg ttgatgcgca tgatagcgag cgcgttcacg ttgtggtgca ggtaaatcag
     2341 aatcgcttca accagcaagg gtagaaaaca gatcgcgaaa aacgcggtga agagttttga
     2401 cttaaagacg tcgcgaaagg catggcgcgg aattatcagg aagcggctcc actccggagt
     2461 cagcgcgcct gcgtaattct tgtagctgtg atcatagact gccatgaccg ttctccattg
     2521 ccttaaggaa gatgtcctcg agcgaatcgc gcttgtagct caaacgcctg atctgcattt
     2581 cctgctcggc agcgatccga tagaggtcgc gcacttctac cgtctcctgt aatacgacct
     2641 tgagccggtg atcgccggta agcgcatact cgcaacctag atcaccaatc gctttagcaa
     2701 atcgcgcctg atcgccgcgc gtttccagca ccagaaactt gcggttcgac ttgcgctctt
     2761 cctccaggtt gcagtaaacg accagacgcc catctttcag aatcacaatt tcctcacagc
     2821 actcttcgac gtcgcgtaac aggtgtgaag acaaaacaat attagcctgg ccactgtcgc
     2881 gaatctcgcg aatcaaacga atcatccgcg cgcgcgccgg aggatcgaga ccattggttg
     2941 gttcatcaag aaaaatcaac ttgggaccgt gagcaattgc ctgcgcaagt ttcgcgagct
     3001 gcttcatgcc cagcgaatag gtatccagac tgcggtagcg cgcttcccca aggccaacat
     3061 agaagagcgc ctcgtgggct cgttccaatg ctgcctctgg cggcaagccg gagagttccg
     3121 ccatcaaacg caccagatgc acggcgctca tcttggcgat aaaggaatcg cgctcgggca
     3181 tatagccgat gagccctctg atctgttttg cgtcggtaag aatgtctttg ccgaaaatgt
     3241 gggcagttcc agacgaaggt ggatgaaagc cgagcaaggt atgaatcagc gttgtcttac
     3301 cggcgccgtt gggaccaagc agaccgatag cacgcccgcg gagctcaccg cggagaccct
     3361 gcagaatttg tcgcttaccg aaagcaacac tcaaatcatt gaggtcgatt acgggagcga
     3421 aatttctggg gtcgtcgagt tttgttaagt cgcttattgc ggctgtcgcc atgctcgtta
     3481 cttatatcgt cctgtcttga taattccaaa cgaatcccaa tcca
//
LOCUS       DASUEX010000680         3041 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_684831, whole genome shotgun sequence.
ACCESSION   DASUEX010000680 DASUEX010000000
VERSION     DASUEX010000680.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_684831"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..2564)
                     /locus_tag="VFV61_11310"
     CDS             complement(114..2564)
                     /locus_tag="VFV61_11310"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX4901112.1"
                     /translation="MTEKHKNGNPLNVVGKPFRKVDARAKCVGQTRFADDIILPRMLY
                     CKMLRSHEPHALIKKIDTSKAAALPGVFAVLTGKDLPIPYGILPVSQDEHALCMDKVR
                     FVGDPVAAVAAIDEDTAFEAMNLIEVEYERLNTISSIEEALLIDEPRIHGYGDGGNIH
                     KKVSLEFGDLEAGFAEADLVREDTFFYEGNTHLPMEQHAAVAHFDSDEKLTLWSSTQT
                     PHYVHRALAKVLQMPASHVRVIATPNGGGFGGKSDPFNHEVVVCKLAIMTGRPVKISL
                     TREEVFYCHRGRHPVLMHVKTGVKKDGSITGMHFQSYLDGGAYGSYGVASTFYTGALQ
                     TVTYEVPRYKFQGIRCFTNKPPCGPKRGHGTPQPRYALEVQLDKIAEQLKLDPAEIRR
                     QHLVKPFSVTANYLRVGSMGLGTCIDKVVEGSDWKNRFSWDTNQRRLPLGKGIGLACS
                     SYICGAGLPIYWNSMPQSGVQLRLDRQGGVCVMCGSTDIGQGSDSILAYIVAEVLGID
                     PFDIRVVTADTDLTPVDLGSYSSRVTLMTGNAAIQAAERARELLVLAVAEKLGVPIEN
                     LSLAERRVFDVENPDVGVTFAEAVVLAESKFGTIGTVGSYSPPASPGKYKGAGVGPSP
                     AYSYSAAVAEVDVDAATGIVTVERVWIAHDIGKSINPMLVMGQVEGGVYMGLGEILME
                     EMSYRANRNVVHKFPSMLEYKSPTTMEMCDVKTYLIEDADPNGPFGAKEVGQGPLLPV
                     PPAVANAVYNAVGVRIDEVPITPEKVLKALREKEKGRAGRYGPDSVPVVDWPEPLKVL
                     TPDEGGDGREMPHVAVHS"
     gene            complement(2628..>3041)
                     /locus_tag="VFV61_11315"
     CDS             complement(2628..>3041)
                     /locus_tag="VFV61_11315"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX4901113.1"
                     /translation="ALTGTKHGCELGECGTCTVLVDGKAILSCLMLGLEAEGHEVKTI
                     EGMADGAELHPLQDTFAETGAAQCGYCSPGFLLVAEELLLKNASPTREEIKEALSGNL
                     CRCTGYLKIYEAVELAAARMRGERAELPKEAVFGL"
BASE COUNT          655 a          904 c          760 g          722 t
ORIGIN      
        1 ccggccccgc tccggccatc accttcagcg cctccgcaat ttcacgcggc gcacggtatt
       61 caaatttcgg taatcgcatc atagctggac aaccctatcg accaatcata aatctacgaa
      121 tgcactgcca catgcggcat ctctctacca tcgccacctt catcgggtgt taaaaccttc
      181 agcggctcgg gccaatccac cacgggaact gaatcgggac cgtaacgtcc tgctcgacct
      241 ttttcttttt ctcgcaaagc ctttaatacc ttttccggtg tgatcggcac ttcatcgatt
      301 cgcacaccga ctgcgttata cacggcgttc gccacggccg gcggcaccgg caagagcggc
      361 ccctgtccaa cttcttttgc gccaaaaggt ccgtttggat ctgcgtcttc gatcagatag
      421 gttttgacgt cgcacatctc catcgttgtc ggactcttgt attccagcat cgagggaaac
      481 ttgtgcacca cgttcctgtt ggcacggtaa gacatctcct ccatcagaat ttccccgagt
      541 cccatgtaca cgccgccttc aacctggccc atgaccagca tcggattgat cgatttgcct
      601 atgtcatgcg caatccaaac gcgctcaacg gtcacaatgc ctgttgcagc atcaacatca
      661 acttcggcta cggccgctga atagctgtat gccggagatg gtccgacgcc ggcgcccttg
      721 tacttcccgg gtgaggcagg gggtgaatag gagcccacgg ttccgatggt gccaaacttt
      781 gattcggcga ggactaccgc ttccgcaaac gtgacgccta cgtccgggtt ctccacatca
      841 aacacgcgtc gctcagcgag cgaaagattt tcgataggca cgccgagctt ttctgctacc
      901 gccaatacca acagctcgcg agctctttct gctgcctgaa tagcggcgtt gccagtcatc
      961 agagttactc gactcgaata acttcccaga tccaccggtg tcaggtccgt atccgcggtc
     1021 actacacgaa tgtcgaaagg atcgattccc aggacctcgg ccacgatgta ggccagaatg
     1081 gaatcggaac cctggccaat gtcggtcgat ccgcacatga cgcagacacc gccctggcga
     1141 tccaaacgga gctgaacacc agactgtggc atgctgttcc agtagatcgg caaccctgcg
     1201 ccacagatgt aagagctgca ggcgaggcca atacccttgc ctaagggcag ccttcgctga
     1261 tttgtgtccc acgaaaacct gtttttccaa tccgatcctt caacgacctt atcgatgcag
     1321 gttcccaacc ccatgctgcc gacgcgcagg taattcgcgg taactgaaaa gggcttcacc
     1381 agatgctggc gcctgatttc agctggatcc aatttgagtt gttctgcgat cttgtccaat
     1441 tgaacttcga gcgcgtagcg aggctgcggg gttccatgtc ctcgttttgg accgcacggc
     1501 ggcttgttag taaagcaacg gatgccttga aatttgtagc gcgggacttc gtaagtcacg
     1561 gtttgcaggg caccggtgta aaacgtgctc gccactccgt agctgccata tgctccacca
     1621 tcgagatacg actgaaagtg catccctgtg attgacccat ccttcttcac ccctgtcttg
     1681 acgtgcatga gaaccggatg gcgaccacga tggcagtaaa atacctcttc acgtgtgagc
     1741 gagattttga ctggccgacc agtcatgatg gcgagtttgc aaaccaccac ttcatgattg
     1801 aagggatcac tcttgccgcc gaaaccgccg ccatttggtg tggcaattac gcgtacatga
     1861 ctcgcaggca tctggagcac cttggcgaga gcacgatgta cgtagtgagg tgtttgagtt
     1921 gaactccaga gcgttagttt ttcatccgaa tcgaaatgtg ctacggcagc atgctgctcc
     1981 atcggcaagt gagtgttgcc ttcgtagaaa aaggtgtctt ccctgaccag gtcggcctct
     2041 gcaaagccag cctcgagatc tccaaactcc agcgagacct tcttatgaat gtttcctcca
     2101 tcaccatacc cgtgaatccg gggttcatcg atcagtaagg cctcttcgat tgaggagatt
     2161 gtattgagcc gttcatactc gacctcgatc agattcattg cctcaaacgc cgtgtcttcg
     2221 tcgatcgccg cgactgcagc gactgggtcg ccgacgaatc ggaccttatc catgcacaaa
     2281 gcatgttcgt cctggcttac gggcaggatg ccgtacggga tcggcaagtc tttgccggtc
     2341 aaaacagcga atactcccgg cagtgccgcc gccttgctgg tatcaatttt cttgattaga
     2401 gcgtggggtt catggctccg caacatcttg caatagagca tccgcggcaa aatgatatcg
     2461 tcggcaaatc ttgtttggcc cacacacttg gcccgcgcat caactttgcg aaacggcttg
     2521 ccaaccacat ttagaggatt tccatttttg tgtttttcag tcattcggtt cttgggcaga
     2581 ccggacagat gcggaccagg ttaagttgca gcgtcgggcg gaatgcttca caacccaaac
     2641 acagcttctt tcggaagctc cgctctttct cctcgcatcc gcgcggcggc cagctctact
     2701 gcctcgtaga tcttcaaata gccggtgcag cgacagaggt tgccggaaag tgcttccttg
     2761 atctcttcgc gcgtcgggct ggcattctta agcaagagct cttctgcgac gagcaggaac
     2821 ccaggtgaac aatagccaca ctgtgccgcc cccgtttcgg cgaaggtgtc ttgcagtgga
     2881 tgtagttcgg cgccatctgc cattccctca atcgtcttta cctcatgacc ctccgcttcc
     2941 aggccaagca tgaggcagga gagaatagct ttaccgtcta ccagcactgt gcaggtgccg
     3001 cactcgccaa gttcacaacc gtgcttggtg cctgtcaacg c
//
LOCUS       DASUEX010000681         7447 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_685212, whole genome shotgun sequence.
ACCESSION   DASUEX010000681 DASUEX010000000
VERSION     DASUEX010000681.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7447
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_685212"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..1420)
                     /locus_tag="VFV61_11320"
     CDS             complement(143..1420)
                     /locus_tag="VFV61_11320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly PilX N-terminal domain-containing
                     protein"
                     /protein_id="HEX4901114.1"
                     /translation="MKTKKGRSKSDCPRHDERGAALLTAVMITTMLLGIAGMVILSTG
                     MSATTSVDSTAELQAYYGAESGLEAVLNVLRGNVAPRSGLPTGTRMGFRSAVQLDKSN
                     LPTDTAAFARLSGWLPYDNANNLVTPAGAGYAYRVILNDPDDPAGTQLAADATYKPRR
                     IKVQSFGFGPRGSMKQMEMVVERVQFDFEARAALAMRGADDNVTPMTFTIGDSAAKDY
                     SGHDFTGGQPALPSFGVTHAVDKLVAEVAITKGSTVEPEQVHIVPISDLPTFLQTADN
                     ARLFLNYMQAVSRSMGRYFTSFNGSAGSSTAPELTFVNGNCNLDGGAGLLIVTGNLVL
                     SGNPNFNGIVLVLGNGYMERDGAGNGNILGTTYVAKFARSWPASENGQPHPFLAPTFI
                     TNGTGNADLRFDSPSVQTGRDVLGTIVRDVREF"
     gene            complement(1436..2110)
                     /locus_tag="VFV61_11325"
     CDS             complement(1436..2110)
                     /locus_tag="VFV61_11325"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX4901115.1"
                     /translation="MNTKLNSVRGELGFSLIELMIAMTLTLIIAGASATLLSQSFRMR
                     SREDIRSDAIADAQRALNIVSREIANSGFGLVDNGIVPGDTNNQSIRIRANLNAYTYE
                     PTNHEVIDPDEDIKYLMYNDDPANRHYLVRLDASLAVTDPPKSKTVLANRLDSFSLRY
                     FDSGGAEIDVVANPLNVRDAWKVRISVGVILPADGTPGSPGYQPQSTVNLASDVVLRN
                     VSQLTY"
     gene            complement(2125..2568)
                     /locus_tag="VFV61_11330"
     CDS             complement(2125..2568)
                     /locus_tag="VFV61_11330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX4901116.1"
                     /translation="MLYCVKKQKKGPQSGFTLLETAVAMVVMMIGGLGIAAVFSYAVK
                     NNNGSRDRAIAIAVAQQEIERLRSVEFNDPALAATPTLVTPITVTNGGRSFSLRTTIL
                     NTTPTLKTIQVRVTPLGNPNAWASRTVQITIQRAAFTLGAYTGGP"
     gene            complement(2593..3630)
                     /locus_tag="VFV61_11335"
     CDS             complement(2593..3630)
                     /locus_tag="VFV61_11335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4901117.1"
                     /translation="MAVEKKNASVTKPGGHRGFTLLQMLIVVVVIAVIGGMAAFGIAQ
                     ARQRIRLTNSARLLASYLEKARVDSIRRHPINAGDMAGVTFLSATRYQVRMDFDGNGT
                     VETRDIDLDSGISLVNNAVPTRFDWRGRFLTNDPNITKVSFTLQYGSSSDQRTVDVTR
                     SGDVTIDSREYMDDVPNVNVNENLSGIDSGSTVNGNYNPSPSPSPTVDPNPSPTPNPP
                     PTPDPSPSPYPSPTTYPTPTPIPSPTASPSSSPSPQPSPSPSPAPCVVTITPPALSIH
                     KNGGVGNLSFTVTGGSGAVSFVSGPGNLTVTETSTYNFQVTSINNSRGDFTLYFNTPC
                     GSRQVTVSVTN"
     gene            3781..4020
                     /locus_tag="VFV61_11340"
     CDS             3781..4020
                     /locus_tag="VFV61_11340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4901118.1"
                     /translation="MEASFPFHGVAGWSVVLKFRELSLFSEGSKLYSAAILPVRGVTN
                     RPLVCGAARRARQLDGKRPVDPRGWRGAKNFQVET"
     gene            complement(4029..5405)
                     /locus_tag="VFV61_11345"
     CDS             complement(4029..5405)
                     /locus_tag="VFV61_11345"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEX4901119.1"
                     /translation="MNSNRTPGFLIAVLAVMLVAGITVPSSAQTQTSEVTRPRTAATP
                     PPAEPSPTPAPEGAAAEQEAIYGLQGVLVETLDGSVVSTQAADDAFNPASALKLATAL
                     VALRTFGPDHRFATGVWTDGSLDKTSGTLNGNLYISGRDPSFHYEHGVMIARQLNALG
                     IRTVNGDLVIGPGFTMNFNWSARTSGARLYETLDASLRSGEATRSWLYERGLLGDKAS
                     LASVPSVAFTGELRIDAVVSNARLLLSHHSSKLVDILKVLLCYSNNFMAERIGDSIGG
                     VQTVRRNLIETLGLTPDQIQMSSLSGLGVNRASPRTMMRIFRALREELRKYRLSTTDI
                     MPVAGIDPGTMETRFTQPPYRGSVIAKTGTLVRTDGGASALVGQMKTASGATLLFVIM
                     NRRGNVLKFRSNQDFLVMQIQSSRGGPSAFSYKPRSLAMKLSDTQSSFASAGDEFEPV
                     IKLSTAPQ"
     gene            5641..6294
                     /locus_tag="VFV61_11350"
     CDS             5641..6294
                     /locus_tag="VFV61_11350"
                     /inference="COORDINATES: protein motif:HMM:NF002305.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosylase/DNA lyase"
                     /protein_id="HEX4901120.1"
                     /translation="MPVTVESIRATHRARKKEIQARLREFEKVWQEGSDERLWEELVY
                     CIFTAGASARMGLKSVAAVRPLLRDGGREEMTQALTQAGAHRFPVARPGYIVVARNYL
                     REQCPHGLRERLQDFSSALERRDWLAQEKQIKGLGYKEASHFLRNIGLKGHAILDKHV
                     LRCLTDLKVVENPKPPTTRARYLATEDKLRTVAQDLKIDFDELDLVLWSMKTGEVLK"
     gene            6328..>7447
                     /locus_tag="VFV61_11355"
     CDS             6328..>7447
                     /locus_tag="VFV61_11355"
                     /inference="COORDINATES: protein motif:HMM:NF025341.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HEX4901121.1"
                     /translation="MSVIRKVVVMRLFSCLGFKRMMAVGVVAAFVVCTELPTATRAAT
                     LDNQKTFKRAQKELREGDFEQAEIILREVLAKDALNNPARLALSFALLKQRRLQEAFD
                     HAARVLASDPASARAHALLGQAILASGDFKESVEEFRTALALDEDEAMAVAGLAMVDF
                     YENRLAECVKGLRKAVSMDGNEPDYIFSLGQASARYEMFKEAADSYERFLAIAHRTDA
                     DRRERIRGLINFLRYLGRQGKLYVSVGRDSTTMSFETIDGRPILKVRINGQREPLRFV
                     LDTGSGMSVVSEQTAKRLGLQVISRGGMARAVGGAGRFEIVYGFLSSLEVGEVRVENV
                     PVYIRHFYDDKVKVDGYLGLSVITRFFASVDYGERTFKL"
BASE COUNT         1645 a         1965 c         2135 g         1702 t
ORIGIN      
        1 caatcaacaa ctcaagcact gaaaagccgg aggcgttagc tgactttgtt gtcgtgagag
       61 gggctttcat gttacttgct cgtccgtcgt gtcaaaacag agacttgact acagttcaac
      121 ccgccgaccc aggactctgg aactagaatt ctcgtacgtc gcggacgatc gttcccaaaa
      181 cgtctctgcc tgtttgcacc gagggagaat cgaaacgaag gtccgcatta ccagtaccgt
      241 tcgtgatgaa ggtaggagcg aggaatgggt gtggctgacc gttttcgcta gccggccagg
      301 atcgcgcgaa cttggccaca tacgtagtac cgagaatgtt accgttgccc gcgccatcgc
      361 gttccatgta accattgccc aacaccagaa cgattccgtt gaagtttgga tttccgctca
      421 ataccaggtt gccggttacg atcaaaagac cagcgccacc atcgaggttg caattaccat
      481 ttacaaaagt gagttctggc gcagtgctgc tgccagcgga tccattaaag gaagtgaagt
      541 aacgacccat gctgcgagac acggcctgca tataattcag gaacagtctt gcattgtcgg
      601 cggtctgcaa aaaagtgggc aagtcgctta tgggcacgat atgaacctgc tcaggctcaa
      661 cagtgcttcc tttggtgatc gcgacttccg ctacgagctt atccactgca tgcgtgacgc
      721 caaatgacgg taaggccggt tgacctccgg taaagtcgtg accgctgtaa tccttcgccg
      781 cgctgtcacc gatggtgaaa gtcatcggag taacgttgtc gtcggcgccg cgcatcgcca
      841 gtgcagctct tgcctcgaag tcgaattgaa cacgttccac aaccatttcc atttgcttca
      901 tcgagccgcg cggaccgaaa ccaaatgact gcacctttat gcgtcttggt ttataggtag
      961 cgtcggctgc gagttgcgtg cccgccggat catccgggtc attgaggatc actcggtagg
     1021 cgtaacccgc tccagctggt gttacgagat tgttggcatt gtcgtaaggc agccagccag
     1081 acagtctggc gaaggcagca gtgtcagtcg ggaggttgga tttatccagt tgcactgcac
     1141 tcctgaatcc catccgcgtt cctgtcggca agcctgagcg gggggcgaca ttccccctga
     1201 ggacattcaa aaccgcttcc agcccagatt ccgcaccata gtaggcttgc agctcagccg
     1261 tcgaatcgac ggacgtagtt gctgacatgc ccgtcgacaa aatcaccata ccggcgattc
     1321 cgagtagcat tgtcgtaatc atgacagcgg ttagcagtgc cgcgccacgt tcgtcatgcc
     1381 gggggcaatc cgacttgctt cggccctttt tcgtcttcat cgaggattcc tcttatcagt
     1441 aagtaagctg actaacgttc cttagcacga catctgaagc gaggttcact gttgattggg
     1501 gctgataacc gggtgaaccg ggtgttccgt ccgcaggcag aataacgcca actgaaattc
     1561 ggactttcca ggcatctcta acattgagag gattggcaac gacgtcgatt tcagcgccgc
     1621 cgctatcaaa atatcggagg ctaaacgaat cgagccggtt cgcaagcaca gttttcgact
     1681 tgggagggtc cgttaccgcc aagctcgcat caagtcgtac gaggtagtgc cggtttgcag
     1741 gatcgtcgtt atacatcaaa tactttatgt cttcatccgg atcaattact tcgtgattgg
     1801 tgggctcgta ggtgtaagcg tttagattgg cgcgaatgcg aattgattgg ttattggtat
     1861 ctccggggac aattccattg tccacaagcc caaagccgga attggcaatc tcgcgcgaga
     1921 caatgttcag cgctcgctgc gcgtcagcaa tcgcatctga gcgaatgtct tcccgcgaac
     1981 gcatgcggaa cgactggctc agcagcgtgg cagaggcgcc cgcgatgatc agtgtcaagg
     2041 tcatcgcgat cattagctcg atcaaggaga agccgagttc gccgcgaact gaattgagtt
     2101 tcgtgttcat taacattcct ctgcttacgg tccaccggta taagcgccca gggtaaacgc
     2161 agcgcgttgt attgtgatct gaacggtgcg actggcccat gcattcggat tgccgagggg
     2221 agtgacccga acctgaattg tcttaagggt cggagtcgtg ttcaggatgg tggtgcgcag
     2281 cgagaagctt cgtccgccgt tggtaaccgt aatgggcgtt accagggtcg gagtggcagc
     2341 cagcgcggga tcgttgaatt ccacacttct cagccgctcg atctcctgct gtgctactgc
     2401 gatcgcaatc gcgcggtcac gcgacccgtt gttgttcttg acggcgtagg aaaagaccgc
     2461 ggcaattccc aaaccgccga tcatcatcac caccatggcc acagcggttt ccagcagggt
     2521 gaaaccactc tgcggcccct tcttctgttt tttgacgcaa tacagcatct aattttctcc
     2581 gtcttgccgt tgttagttgg tgacgctgac tgttacctgg cgcgagccgc acggtgtgtt
     2641 gaaatacaac gtgaagtcgc cgcgcgaatt gtttatcgaa gtgacctgga agttgtacgt
     2701 cgaggtctct gtgaccgtca ggttgccagg tccgctaacg aacgaaacag cacctgaccc
     2761 gccggtgacc gtgaagctca ggttccccac gccaccgttc ttgtgaatcg agagcgctgg
     2821 aggagtgatg gtcacaacgc aaggcgccgg acttggcgat ggcgacggct gcggacttgg
     2881 tgacgaactg ggtgaagccg ttggagatgg aatcggcgtt ggagtcggat aagtcgtggg
     2941 cgatggatag ggcgatggcg acggatcagg cgtcggcggg ggattaggtg tcggtgacgg
     3001 gttcggatca acagtcggag atggggaagg gctcggattg tagttaccat taaccgttga
     3061 acccgaatcg atgccgctca gattctcatt aacatttacg ttgggaacat cgtccatgta
     3121 ttcgcgggaa tcgatcgtca catcgccgga acgggtgacg tcgaccgtgc gttgatcaga
     3181 acttgatcca tactgcagcg taaaactcac cttcgtaata tttggatcgt tagttaagaa
     3241 tcgtcctcgc caatcgaatc gtgtgggaac tgcattgttt acgaggctga taccgctatc
     3301 gagatcgatg tcacgagtct caactgtgcc atttccatca aagtccatgc ggacctggta
     3361 ccgcgtagcg ctgagaaacg tcaccccggc catatcaccc gcgttgattg ggtgtctgcg
     3421 aattgaatct acccgggctt tttccaggta gcttgcgagc aagcgggcag agttagtcag
     3481 gcgaattcgc tgacgcgcct gagcgatacc aaaggccgcc atcccgccaa tcacggcaat
     3541 cacgacgacc acgattaaca tttgcaacag agtgaaacct ctgtggccac caggcttcgt
     3601 tacgctcgca tttttctttt cgacggccat tgctcctccg agtgccgggg aatcgcaagg
     3661 tggaacctcg cgccgctcta tcggcaacga ccatgccagc agcaacttgg ccaaaaatca
     3721 gcgatttatt cgaggtaccc aaggaaggac ggcgttaatc cgttacccac ggtgacacca
     3781 gtggaagcca gcttcccatt ccatggcgtg gcgggctggt cggtagttct gaagtttcgt
     3841 gagctttcgc tatttagtga aggtagcaag ttatacagcg ccgcaattct tccagtacgc
     3901 ggcgtgacga atcgtccgct cgtgtgtggt gccgcacgcc gcgcgcgtca gttggacggt
     3961 aagcgccccg tcgatcctcg tggttggcgg ggcgcaaaaa atttccaggt tgaaacttaa
     4021 ggttggcctt actggggagc agtagagagc ttaatgacgg gctcaaactc atcaccggca
     4081 ctggcgaagc tgctttgagt gtcagacagt ttcatagcca gcgagcgcgg tttatagctg
     4141 aaagccgagg ggccgccacg agagctttga atctgcatca cgagaaaatc ctggttcgag
     4201 cgaaacttca agacattgcc gcggcggttc atgatgacaa agagtagcgt tgcgccactt
     4261 gcagttttca tctgaccgac aagtgcactg gccccgccgt cagttctcac taatgttccg
     4321 gttttcgcaa tcacacttcc gcggtaggga ggttgcgtga agcgcgtttc catcgtcccg
     4381 ggatcgatgc cggcgacggg catgatgtca gttgttgaaa gcctgtattt ccgtaattcc
     4441 tcgcgcaggg ctcggaagat cctcatcatc gttcgcgggc tggcgcgatt aacacccaaa
     4501 ccgctcaacg aggacatctg aatctggtcc ggtgtcagcc ccagggtttc aatcaggttt
     4561 cgtcgcactg tctgcacccc gccgatcgaa tcgcctattc gctcggccat gaagttgttg
     4621 gagtaacaga gcagtacctt gagaatgtca accagcttgc tggaatggtg tgacagcagc
     4681 agtctggcat ttgaaaccac cgcatcaatg cgtaactcac ctgtaaacgc cacactgggc
     4741 acgctggcca gactggcttt gtcgcccagg agccctcgct cataaagcca tgatcgcgtt
     4801 gcttcgccgg aacgaaggct cgcgtcaagg gtctcataca gtcgggcacc agaggtgcgc
     4861 gcggaccagt tgaagttcat cgtaaatcca ggtccgatta ctaagtcgcc gtttaccgtg
     4921 cgaatgccca gcgcgttgag ttggcgcgcg atcatcacgc cgtgctcgta gtgaaaagat
     4981 ggatcgcggc cggaaatata aagattgccg ttaagtgtgc cgctagtctt atcgagggag
     5041 ccatcggtcc aaacccctgt cgcaaagcgg tgatctggtc caaatgtgcg caaggccaca
     5101 agcgccgtcg ccagctttaa agcggaagcg ggattgaatg catcgtccgc cgcttgcgtg
     5161 gaaacaaccg aaccatcgag ggtctctacc agcactcctt gcagtccgta gatcgcttcc
     5221 tgttctgctg cggcgccttc tggggcgggc gttggactcg gctcggctgg cggcggcgtc
     5281 gccgcagttc tggggcgcgt aacctcggaa gtttgagtct gggccgagga gggcacagta
     5341 atcccggcca cgagcatcac tgccaacacg gcaatcaaga acccaggtgt gcgattgcta
     5401 ttcattcgat ctcttgccaa cgccgctccg gctgcgccgc ttattaatca taacatgatc
     5461 gtttgggaga gaattccaga aggggcgtga ggagaacgtg gtctgatgcg cggcgacggc
     5521 gcaaagatgc acggcatttg cagtgtgcaa agtcctgcga cgccgcggga taattgcgtt
     5581 gacaaggaaa gaatccgaca cctagaatct cgctattgac aaatcaaagt gcgcaatcac
     5641 ttgcccgtca cagttgagag tattcgagcc acgcatcgcg cgcgcaagaa agaaatccaa
     5701 gcccggctgc gcgagtttga aaaagtctgg caggaaggtt ctgacgagcg cctctgggaa
     5761 gaactcgtct actgcatctt taccgccggc gcgtcagcac gcatggggtt gaaatccgtc
     5821 gctgcggtgc gaccactgtt acgcgatggc ggccgcgaag agatgacgca ggctttgacc
     5881 caggctggcg cccatcggtt tcccgttgcc cgacccggct acattgtggt agcgcgaaac
     5941 tatttgcgtg aacaatgtcc acacggtttg cgcgagcgac tgcaagactt ttccagcgcg
     6001 ctcgaacgtc gtgattggct ggcgcaggag aaacagatta agggcctggg ctataaagaa
     6061 gccagtcatt tcctgcgcaa tatcggcttg aaaggccacg caattcttga caagcacgtg
     6121 ttgcggtgtc tcactgacct taaggtggtc gagaatccaa agccaccgac aacgagggcg
     6181 cgttatctgg caactgaaga taaactacga acggttgccc aggatctgaa gatcgatttt
     6241 gatgaacttg atttggtgct ttggtcgatg aaaacggggg aagtcttgaa gtaatagttt
     6301 taggatgacg aagtatgcat ctcattgatg agcgtcattc gaaaggttgt ggtcatgcgg
     6361 ttgtttagtt gtctcggctt taagcgaatg atggcggtcg gagttgtggc cgcctttgtc
     6421 gtttgcactg aactccccac cgcgactcga gcggcgacgc tcgataacca gaagacattc
     6481 aaacgcgccc agaaggaatt gcgcgaagga gactttgagc aggctgaaat aatcctgcgc
     6541 gaggtcttgg cgaaggacgc gctgaacaac cctgcgcgtc tggcactgag ttttgcgctg
     6601 ctcaaacaaa gacggctgca ggaagccttc gatcatgcag cgcgtgtcct tgcttctgat
     6661 ccggcttcgg ctcgtgcaca tgctctcctg ggacaagcga ttcttgcttc gggagacttt
     6721 aaagaatcgg tcgaagagtt tcgcactgct ctggcccttg atgaagatga agcgatggcc
     6781 gtagccggcc tcgcgatggt cgatttctac gagaaccgtc tcgcggagtg cgttaaggga
     6841 ctgcgtaagg ccgtgagtat ggacggcaat gagcctgatt acatttttag tctgggtcaa
     6901 gcgtctgctc gctacgagat gttcaaagag gcggctgatt cgtacgaacg ctttctcgcc
     6961 atcgcgcaca gaaccgacgc tgatcggcgg gaaagaattc gcggtctgat caatttcctg
     7021 cgttacctcg ggcggcaggg aaagctttac gtttccgtcg gacgcgattc aactaccatg
     7081 tcgttcgaaa cgatcgacgg acgtccgatc ttaaaagtcc ggattaatgg tcagcgggaa
     7141 cccctgcgtt tcgtgctcga cactggctcg ggaatgtcag tggtctcgga acaaaccgcc
     7201 aagcgtctgg gcttgcaggt tatctcccgt ggaggcatgg cccgcgccgt gggcggggcc
     7261 ggtaggtttg agattgtcta cggctttctt tcttcattag aagtgggaga agtccgcgtc
     7321 gaaaacgtgc cggtgtacat ccgccacttc tatgacgaca aggttaaggt agatggttat
     7381 ctcggtctct cggtgatcac caggttcttc gcctcagtag actacggcga gcgcaccttc
     7441 aaattga
//
LOCUS       DASUEX010000682         3148 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_563867, whole genome shotgun sequence.
ACCESSION   DASUEX010000682 DASUEX010000000
VERSION     DASUEX010000682.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3148
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_563867"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..899)
                     /locus_tag="VFV61_11360"
     CDS             complement(78..899)
                     /locus_tag="VFV61_11360"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX4901122.1"
                     /translation="MKLETFPGKRGQAALPYCAFITSGVGSSVKGVSMKKAMAISFLV
                     ILSLGVNALAQGKSQDKAKTPPAHKMVEFQMALLKRTPNWEEIKTTNPMALERAQMNY
                     VNALIERGNAVIAGRLTDDGDIRGVYVLRAKSVAEAQEWADNSPAVKSGHLIAEIHPW
                     WSEEVMKRTATPEVMTTAYLAFLTRGEKWTPEKTPQTEAIQKAHMDNINRLAETKKLV
                     VAGPFGDNGTLRGIFVFKVASLDEARALAETDPAVQAGRLALDIHPWLVPEGILP"
     gene            complement(920..2047)
                     /locus_tag="VFV61_11365"
     CDS             complement(920..2047)
                     /locus_tag="VFV61_11365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4901123.1"
                     /translation="MPETNHVTPKSGDQLLLVGTMKGAFVLKSDANRRNWEVGGPYFP
                     GRAIYAMNYDGRAGRNKLWAAVNSSFWGSYLSASQDFGKTWTEPETYGVKFPDDTETS
                     LKQIWQIVPGNSQEPQTLYCGVEPAALFRSDDAGETWSLVKGLHDHPHRPQWQPGGGG
                     LCLHTILPDPVNKDRLHIAISTGGVYRTDDGGKSWEARNVGVRAQFLPEDQRYPEFGQ
                     CVHKIVSHPSKPERLFLQNHWGLYRSDDAGDSWTDIANGVPSDFGFALEIDHNEPNTA
                     YIIPLESDEFRCTPEPKLRVYKTSNAGDSWQPLTNGLPQENAFETVLRDGMAGDTKQP
                     MGIYFGTRNGKLFGSNDAGNSWYAIEEGLPQVVCVKTATVA"
     gene            complement(2231..>3148)
                     /locus_tag="VFV61_11370"
     CDS             complement(2231..>3148)
                     /locus_tag="VFV61_11370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4901124.1"
                     /translation="AKNGAEKPRGSGPVRSTPIALLRRRDFALWNSVFPRPTVTELTF
                     STTTQVVYDYLQARGASFFNDIVEGAKLLRSQVEESLAELVAQGLVVSDSFTGLRALL
                     TPSHRKTNAAARRKHRQPVYDMATAGRWSILERGSGDAVDPEVAEKLARIFLKRYGVV
                     FKRLLEREGVTIPWRVLLRTYHRLEARGEIRGGRFVAGISGEQFALPEAVGLVRSIRR
                     APAVETMISVSAADPLNLVGIITPGGRITAHASNRVLYRDGAAVAVLEAGETRFLVEL
                     SRAAEWKAKAALLRKATPPVLRTYLRRPA"
BASE COUNT          698 a          937 c          817 g          696 t
ORIGIN      
        1 aaccaacttg cgaagcaaaa ttgatccctg agagatacgc caatcggtca attggcaatt
       61 ggcaatcggc aatctgccta cggcagaatc ccctccggca cgagccacgg atgaatatcc
      121 aaagcaagcc gacccgcctg caccgccgga tcagtttcgg ccagcgccct ggcttcatcc
      181 agcgacgcca ctttgaaaac gaagatgccg cggagcgtac cattgtcgcc aaagggtccg
      241 gctaccacca gcttcttcgt ttctgccaga cggttgatgt tatccatatg cgccttttga
      301 atggcctccg tctggggcgt cttctccggc gtccactttt cgccgcgcgt cagaaaagcc
      361 aggtacgcag tcgtcattac ttccggtgtg gccgttcgct tcatgacttc ttctgaccac
      421 cacgggtgta tctcggcaat caagtgaccc gacttcaccg ccggactgtt atcggcccac
      481 tcctgcgcct ccgcgaccga ttttgcgcgc aaaacataga caccacgaat gtcgccgtcg
      541 tcagtcaatc ggccagcaat tactgcgttg cctcgttcaa tcaaggcatt cacataattc
      601 atctgcgccc gttcgagcgc catcgggtta gtagtcttga tttcctccca gttcggcgtt
      661 cgcttcaaga gcgccatttg gaattcaacc atcttatgtg caggcggggt cttcgcttta
      721 tcttgcgact tgccctgcgc caaggcatta acgcccagac tcaggattac caggaatgaa
      781 atggccatcg ctttcttcat tgaaacacct ttcacgcttg aacccactcc gctggtaatg
      841 aacgcgcagt aaggaagggc ggcttgcccc cgcttacctg ggaaagtttc gagtttcaag
      901 tttcgcgttt caagtttcac tacgccacgg tcgccgtctt cacgcaaaca acctggggca
      961 gaccctcttc tatcgcatac caggagttgc cggcatcgtt tgaaccaaac agcttgccgt
     1021 tacgcgtgcc gaaatagatt cccatcggct gtttcgtatc gccagccata ccatcacgca
     1081 agaccgtttc aaacgcgttt tcctgcggca gaccgttcgt taaaggttgc cacgaatcac
     1141 ctgcattact ggttttgtaa acgcgcagtt tgggttctgg agtacaacga aactcatccg
     1201 actcgagggg aatgatgtag gcagtgtttg gttcgttgtg atcgatctcg agtgcaaatc
     1261 caaaatccga aggcacgccg ttggcaatgt cagtccagga atcgccggca tcgtcactgc
     1321 gatagagtcc ccaatggttc tgcaaaaaga gccgctctgg ttttgaagga tgactgacaa
     1381 tcttgtgtac acactgtcca aattccggat agcgttggtc ctctggcaga aactgcgcgc
     1441 gcacaccaac gttgcgcgct tcccatgatt tcccgccatc atcagtgcga tagacaccgc
     1501 cggtggaaat agcgatgtgc aggcgatcct tgttgaccgg atcaggaaga atcgtgtgca
     1561 gacacaaacc gccaccaccc ggttgccact gcggccggtg cgggtgatcg tgcagacctt
     1621 taactaacga ccacgtttct cccgcgtcat ccgatctaaa caaagcggcg ggctcgaccc
     1681 cgcaatagag tgtctgcggt tcctgagaat tccctgggac gatttgccag atctgcttca
     1741 gcgaagtctc ggtgtcgtcg ggaaatttga cgccatatgt ctccggctca gtccaggtct
     1801 ttccgaaatc ttgggaggca ctgagatatg aaccccagaa agaactatta accgcggccc
     1861 agagtttgtt gcgaccagcg cggccgtcat agttcattgc gtagatggcg cgcccaggaa
     1921 aatacgggcc gccgacttcc cagtttcttc tgttcgcgtc tgacttcaga acgaatgcgc
     1981 ctttcatggt gccgactaac aagagttgat caccggactt cggggttaca tgatttgtct
     2041 cgggcatttg tgctactcct gaatgaattt gatcatcgtc gcatcaccgc caagccggca
     2101 agaatcgcca agaatcgcct agaacaatcc tgcggcgagg ctgaaagaac tagattcaac
     2161 atcgatgcct tggaaccttg gcgaacttgg cgattcttgg cggcttggcg gtgttaagct
     2221 cgcatcgaaa ttacgccggt cgccgaagat acgtcctcaa tactggcggt gtggctttcc
     2281 gaagcaatgc tgctttcgcc ttccactcgg cggcacggct tagctctacc agaaaacgag
     2341 tctcgcctgc ttccaacacg gctaccgcgg caccatcacg ataaaggact cgatttgaag
     2401 catgtgctgt gatgcgacca ccgggcgtga tgataccgac gaggttcaaa gggtcagcag
     2461 cgctcaccga aatcatcgtt tccaccgcgg gcgcacgccg aatcgagcgc acgagtccca
     2521 cggcttccgg aagcgcaaac tgctcccccg agattcccgc gacaaaccgg ccgccgcgga
     2581 tctcgccccg cgcttccagg cgatgataag tgcgtaacaa cacgcgccag ggaatcgtca
     2641 caccttctcg ctcgagcaaa cgcttgaaga ccacgccata ccgtttcaag aaaatgcggg
     2701 ccagcttttc tgccacttcc ggatcaacgg cgtccccact gccgcgctcg agaatcgacc
     2761 agcgtccggc cgtggccatg tcgtaaacgg gttggcggtg cttgcgacgc gcagcggcgt
     2821 tggtcttacg atgagatgga gtcagcagag cgcgcaagcc ggtaaaactg tcggagacaa
     2881 cgagtccctg ggcgactaac tccgcaagcg attcttcaac ctgactgcgc aggagtttcg
     2941 cgccctcaac aatgtcatta aagaaagatg ctccgcgcgc ctgcaaatag tcgtaaacca
     3001 cctgggtcgt ggttgagaaa gtgagctctg taactgtcgg ccgcggaaac acggaattcc
     3061 aaagcgcgaa gtctctacga cgcagcagcg caatcggcgt tgagcgcacg ggtccggaac
     3121 cgcgcggctt ttccgcgccg ttcttcgc
//
LOCUS       DASUEX010000683         2413 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_563902, whole genome shotgun sequence.
ACCESSION   DASUEX010000683 DASUEX010000000
VERSION     DASUEX010000683.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_563902"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(48..971)
                     /locus_tag="VFV61_11375"
     CDS             complement(48..971)
                     /locus_tag="VFV61_11375"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX4901125.1"
                     /translation="MIKRKHDRIVISLVALWVLFTVTLAGWWLMFGLQQLDRLKQVQL
                     EGAVDFQNHYQMLIWEGGVLIVSLIGAGIALLYYARREQKRHAQVEEFFAAFTHDAKT
                     ALASLRLQAESLREDSAGNEPNPLLDRLLSDTVRLQLQLENSLFLVNLPSGKLFVEPV
                     SLSHLIAALRHHWPDLTLTQRGDGVVHADVRALESVLANLIQNAVNHGQATSVSFDID
                     EDPKGRLRVRIADNGRGFNGKFDQLGKLFVRHARNSGTGVGLYIARQLLKRMNSELSF
                     HPGDSGGFVAELSFPAAATNGRLVQTVGDEL"
     gene            complement(1008..2333)
                     /locus_tag="VFV61_11380"
     CDS             complement(1008..2333)
                     /locus_tag="VFV61_11380"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: protein motif:HMM:NF000818.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEX4901126.1"
                     /translation="MASQEAGLATHDLSAKYFARASRLSPGGVHSPVRAFKGVGGEPI
                     FFRSAQGATMTSVDGRQYIDFCQSFGPLMLGHRDEEVSAVVEEAVATAWTFGACEPYS
                     LELAEWITSRLPWVEMLRFVSSGTEAVMSALRVARAATNRQKILKFEGCYHGHVDSLL
                     VKAGSGLAGISASSSAGVSAAVAAETLVSPLDDEESVRAIFETHGADIAAVILEPLPA
                     NYGLLIQRREFISETVKLAREYGSLVIFDEVISGFRVALGGMAEVLGLCPDLVCYGKV
                     IGGGFPVGCYGGRADLMELVAPSGPVYQAGTLSANPVGMLAGLATLQKIERVNAYAQL
                     DQKTADFCDRINQDLQRRGLPFQVTRAASIFWLHLQADGVIRRLQQIPRDHAEKFAGV
                     FHAALASGVYLPPSGHEVCFMSLAHSDELLAHAHGAILAAVEAAAAAKS"
BASE COUNT          578 a          743 c          610 g          482 t
ORIGIN      
        1 gagagtcgcg cccagcgatc gatcatcctc gacgagcaat aaccgcttca taactcgtct
       61 cctaccgtct gcaccaggcg cccgttagta gcggcagcag gaaaacttaa ctcggcaaca
      121 aaaccgccgc tgtcaccggg atgaaacgaa agctcgctat tcatgcgctt gagcaattgc
      181 cgcgcgatgt aaagtcccac gccggtgccg ctgtttcgcg cgtgccgcac gaacaacttg
      241 ccgagctgat cgaacttccc gttaaagccg cggccattgt cagcaatcct gactcgcagc
      301 cgtcccttcg gatcttcatc aatgtcaaaa ctaacgctcg ttgcctgacc gtgattgact
      361 gcgttctgaa tcagattcgc cagcacactt tcgagcgctc gcacatctgc atgaacaact
      421 ccgtcgcctc gttgcgtgag ggtcagatca ggccagtgat gccgcagcgc ggcaatcaaa
      481 tgactcagac ttaccggctc tacaaaaagt tttccgctgg gaagattgac gagaaacagc
      541 gagttttcca gttgcagctg caagcgcact gtgtcgctga gcaaccgatc caacaatggg
      601 ttgggctcat tccctgccga gtcttcgcgc agactttcag cctgcaaacg caaactcgcc
      661 aaagctgtct tcgcgtcgtg ggtgaaggcc gcgaaaaact cttctacttg agcatgtcgc
      721 ttttgctcgc ggcgggcgta atacaaaagc gcaatgccag cgccaattag cgagacaatt
      781 aagacgccgc cttcccaaat gagcatttgg tagtggttct gaaagtctac ggcaccttcg
      841 agttgaacct gcttgagtcg atcgagttgc tgcagaccaa acatcaacca ccagcccgca
      901 agtgtcactg tgaataacac ccagagggct accagcgaga taacaattcg atcgtgcttt
      961 cgtttaatca agactactgg agtgaaaaac tcccctcttg ctcaagctca ggactttgct
     1021 gctgcggccg cctcaaccgc tgccagaatc gcaccgtggg cgtgcgctag caattcgtcg
     1081 gagtgtgcca gcgacatgaa acacacttcg tgaccgctcg gtggtagata gactccactc
     1141 gcaagtgcag cgtgaaagac tccggcaaac ttctccgcgt gatcgcgcgg aatttgctgc
     1201 aagcgtcgaa tcacaccgtc agcttgcaaa tgcaaccaga agatcgacgc cgcacgcgtc
     1261 acctgaaatg gcaagccgcg ccgttgcagg tcctgattga tgcggtcaca gaaatcagcc
     1321 gtcttctgat ccagttgcgc ataagcattg acgcgttcga tcttctgtaa cgttgccaga
     1381 cccgcgagca tccccaccgg gttggcgctt aatgttcccg cctgatagac cggccccgat
     1441 ggcgccacca attccatcag gtccgctctg ccgccgtagc atcccaccgg gaatccaccg
     1501 ccaattactt tgccgtaaca aacaaggtcg ggacacaacc ccaggacctc agccatgcca
     1561 cccagggcga cgcgaaatcc ggaaattact tcgtcaaaga tcaccaggct gccgtactcg
     1621 cgcgcgagct tcacggtttc tgaaatgaat tcccgacgct gaatcaacaa gccgtaattc
     1681 gccggcagtg gttccaggat caccgcggcg atgtccgcgc cgtgtgtctc aaagatagcg
     1741 cgtacagatt cttcatcgtc aagcgggcta acaagcgttt ccgccgcgac agccgcagag
     1801 actccggcac ttgaacttgc tgaaattccc gccaggccgc tccctgcttt gactaagaga
     1861 gaatcgacgt gaccgtgata gcagccttca aacttcagaa tcttctgccg gttggtagca
     1921 gcccgcgcca cccgtaaagc actcattacg gcctcggtgc cagaggagac aaaacgcagc
     1981 atctcaaccc agggcagtcg cgacgtaatc cattcggcca gctcaagcga gtaaggctcg
     2041 caagcaccga aagtccaggc agtagccact gcttcttcca caacggcgct cacttcttcg
     2101 tcgcgatgac cgagcatcaa aggaccaaag ctctgacaga agtcgatgta ctgccgcccg
     2161 tccaccgatg tcatggtcgc gccttgagcg ctgcgaaaga aaatcggctc gccgccgacg
     2221 cccttgaaag cccgaacagg actgtgcacg ccgccggggg acagacggct ggctcgcgca
     2281 aaatatttcg cggaaagatc gtgagtcgct aaacccgctt cttgtgaagc catatctagg
     2341 ttgattcgaa acgactaatt gccgatgccc agtcgccgcg cataacgagc gccatacgta
     2401 ataatgaact gcg
//
LOCUS       DASUEX010000684         2569 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_564134, whole genome shotgun sequence.
ACCESSION   DASUEX010000684 DASUEX010000000
VERSION     DASUEX010000684.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2569
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_564134"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            160..1026
                     /locus_tag="VFV61_11385"
     CDS             160..1026
                     /locus_tag="VFV61_11385"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX4901127.1"
                     /translation="MRALFTGGSSQLGTRVLQCVLEDKQYDEVWCLKHERAVPLSHPK
                     LRVLEWDLKDGSDLHEIPPPLTQVIHFAAATHARVERKYWEINFEGTVKLMEAARSLG
                     CRRFFYASTRCATQGSGAYGESKLAAELELKKVDWESLVIVRPAEIYGLAAQEGIDRM
                     LQVAARFHFVPLLWGSRKVQFAPIHADDFVAIACALLSEQRSGLHTLDICGPEPLNGI
                     AIASRIARYYRALPIPMWWPAVRLLLQGFQSLGIGPVTPDQIKRLEGRKTATVSSTDG
                     TLDRSMKRFLRD"
     gene            1068..1871
                     /locus_tag="VFV61_11390"
     CDS             1068..1871
                     /locus_tag="VFV61_11390"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX4901128.1"
                     /translation="MSDVPEQIENIEKVASAHLDAGMQRHTYPIMYEVEDAHWWYAGR
                     RRIIASLLETICTTLDAHPPAILDVGCGTGANLDLLQRFGEVEGVDISDAALAFCRQR
                     GYTNVRQGAAQLLPYEDNVFDVITALDVVEHLDDDVASLREMRRVLKPNGRALLFVPA
                     FKFLWGVQDDVSNHRRRYTLSQLNSAASEAGFEIERASYANISFFVPIFLVRTFMRIT
                     RLRPATELKINIPAFNGLLGSIFGAERFWLRRGSFPFGVSAFCVVRRLD"
     gene            1884..>2569
                     /locus_tag="VFV61_11395"
     CDS             1884..>2569
                     /locus_tag="VFV61_11395"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX4901129.1"
                     /translation="MKKSLLQYLACPSCGGDIHLTAGSEDQDREILDGVLSCRSCARD
                     WQIVRGVPRFVQLDQLDEEMAATASSFGWQWQHFTQEDERYGEQMLGWLQPVTPEFFQ
                     GKVVLEGGCGKGRHTQLAAKWGARDVIGVDLSDAVEPAFTATRGSEGMHIIQADLCQL
                     PLKQLFDYAFSIGVIHHLEDPQAGFQSLASKVKPKGHLSVWIYGAENNGWITRFVDPL
                     RTRFTSGMNRR"
BASE COUNT          579 a          628 c          748 g          614 t
ORIGIN      
        1 agacagggtt tcgcacaggc tttcaacaaa cggaatgatt gtctttccgc tgcggtagca
       61 cagcacaacc agggagaaat ccgggtcatc cacgaaactt atggtatcta ccgatttagt
      121 cattagcaat ctcgtgcttc caggcgcgct tatagatcaa tgagggcatt gttcacggga
      181 ggctcgtcgc aattgggcac gcgcgtactt cagtgcgtac tcgaagacaa gcaatatgat
      241 gaagtttggt gcctcaagca tgagcgagca gtgcctctca gccaccccaa actacgagtc
      301 ttagaatggg atttgaaaga tggatctgat ctccatgaga taccgccacc cttaactcag
      361 gtcattcact tcgccgcggc gacgcatgct cgagttgagc ggaagtactg ggagataaac
      421 tttgagggca cagtgaagct catggaagct gcgcggtcgt tgggttgtcg ccgctttttt
      481 tatgccagta cgcgttgcgc cacacaaggt agcggagcct acggggaatc gaaactcgcc
      541 gccgagctgg agttgaaaaa agtcgactgg gagagcctgg taatagtgcg accagctgag
      601 atttatggac ttgcggccca ggagggaatc gatcgtatgt tgcaagtagc cgcccggttt
      661 cacttcgtgc cgctcctgtg gggatcccgc aaagtgcagt ttgctccgat tcatgccgat
      721 gactttgtag caatcgcgtg cgcgctcttg agtgaacaac gaagtgggct gcatacactc
      781 gacatctgtg gtcctgaacc tctgaatgga atagcgatcg cctcgcgcat tgcgcgctac
      841 tatcgtgcgt tacccatacc tatgtggtgg cccgcggttc gcttgctttt acaagggttt
      901 cagtctcttg ggataggccc ggtcactcca gaccagatca agcgactcga gggaaggaaa
      961 accgcgacag tctcgagcac ggacggcacg ctcgatagat caatgaagcg tttcttgcgc
     1021 gactaaacgg atgggggcga cttttttgct tagccgctga ggtattgatg tctgacgtac
     1081 cggaacaaat agagaacatc gagaaagttg cgagcgcgca ccttgatgcg ggcatgcagc
     1141 ggcataccta cccgatcatg tatgaggttg aggatgctca ttggtggtat gccggtcgcc
     1201 gccggatcat cgccagtctt ctggagacaa tctgtacaac cctggatgcc cacccacctg
     1261 ctattctgga tgtaggttgt ggcacgggag caaacctgga tctgctgcag cgttttggtg
     1321 aagtagaagg cgttgacatc tcagatgctg cgttagcgtt ttgccggcaa cgtggatata
     1381 caaatgtgcg acaaggtgcg gcccaactgc tgccttatga agacaatgtc tttgacgtaa
     1441 tcacggcgtt ggacgttgtg gaacacctgg acgatgacgt tgcaagcctg cgggaaatga
     1501 gacgagtatt aaagcctaac ggacgcgcac tcctgtttgt gcctgccttt aagtttctct
     1561 ggggcgtgca ggatgatgtg agtaatcaca gacgacgcta tacgttaagc cagctcaata
     1621 gtgccgcgtc cgaagcggga ttcgaaatcg agcgcgccag ctacgccaac atcagtttct
     1681 tcgttcctat attcctggtg cgaaccttca tgcgaataac gaggttgcga ccggcaacgg
     1741 aactcaaaat aaatattcct gcttttaacg gtttgctggg cagcattttt ggagcggagc
     1801 ggttctggtt gcggcgcggc agttttcctt ttggcgtctc ggcgttctgc gtcgttcgcc
     1861 gtttggattg atctcctcag gcaatgaaga aaagtttact tcaatatctg gcgtgcccct
     1921 cgtgtggcgg agacattcac ctgacggctg gcagcgagga tcaggatcgg gagattctcg
     1981 acggcgtgtt gagctgcaga agctgcgcga gagattggca aattgtccga ggcgtgccgc
     2041 gcttcgtgca gctcgatcaa cttgatgaag aaatggccgc gacagcgtcg agctttggtt
     2101 ggcaatggca gcacttcact caggaagacg agcgttatgg tgaacagatg ttgggttggc
     2161 tgcagccggt aacgccggaa ttctttcaag gcaaggtggt gctggaaggt ggatgcggta
     2221 agggcaggca tacgcaactc gcagcaaaat ggggtgcacg cgatgttatc ggtgtcgact
     2281 tgagtgatgc tgtcgagcca gcttttacgg caactcgcgg ctcagagggc atgcatatca
     2341 ttcaagccga cctctgccag ctgccactta agcagctgtt tgactatgcg ttttcgattg
     2401 gcgtgattca tcacctggaa gacccgcaag ctgggtttca atcgcttgct tccaaggtca
     2461 aaccaaaggg gcatttgtca gtttggattt acggagcaga gaacaacggt tggatcacac
     2521 gtttcgttga tcccttacgc acgcgcttta cctccggaat gaatcgccg
//
LOCUS       DASUEX010000685         2534 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_564605, whole genome shotgun sequence.
ACCESSION   DASUEX010000685 DASUEX010000000
VERSION     DASUEX010000685.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2534
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_564605"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..428
                     /locus_tag="VFV61_11400"
     CDS             <1..428
                     /locus_tag="VFV61_11400"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEX4901130.1"
                     /translation="QLLLYSRDKLMRTYRLGLGLSPVGDKVRQGDRRTPEGEFYIFTK
                     NDKSAFYLSLGVSYPNGEHAARGLRAGLIGKAQYDSIIRALRAKRTPPQNTTLGGDIY
                     IHGNGAGSDWTWGCVALENEDIEELYQAIAVGTPVTIKP"
     gene            425..1492
                     /gene="dusB"
                     /locus_tag="VFV61_11405"
     CDS             425..1492
                     /gene="dusB"
                     /locus_tag="VFV61_11405"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEX4901131.1"
                     /translation="MTEIKPFKIRDVVITPPLILSPMAGVTDVSFRRLLKRCGGVGLT
                     VSEFISVEGLTRNNPKSKRQMRFYEDERPFAAQIFGGQSERMVMAAQMAEEVGADILD
                     INCGCPAPKVVKHGGGSGLLKDHTRLESILNEIKKAIKIPLTIKIRAGFYDHTINAVE
                     TAKLAEACGVEHIALHGRTKEQGYRGLANWDLVKQIKETVKVPVSGSGDVTTVEGAFA
                     RFRETGCDGVLIGRGAMANPWIFRQIEDAMHGREMFRPTLADKRALLLDYFVMLREDM
                     PETPAINRMKQLAGQFTRGLQGGALFRTSLYHSHSVAEILERIEEYFEAIEVGKPYFG
                     EAGAPVEEAPELDSCEAASAA"
     gene            complement(1626..>2534)
                     /locus_tag="VFV61_11410"
     CDS             complement(1626..>2534)
                     /locus_tag="VFV61_11410"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEX4901132.1"
                     /translation="VNIPAYRMDVFRDGNLIKSYKIGIGYPQFPLPTGLRKAQTLIFN
                     PTWTPPDEPWVANMRNIAVGEKVPAGSPLNPLGPIKIPIGMPSLIHGGKPLAKIGTFA
                     SHGCVGLTNAQVKDFAKTLAGAVGKEVSDQTIAGYLRDSTKTRSLKLDAPVPVELRYE
                     TIVVEDGKLYIYKDVYDQTTNTEENLRVTLAKNNVRFEDFSAEERDQILEALNAMSSR
                     PQKQVPKAANANASPATPTNNGNENSAVANAGEKKPKTPRKPISRKQKQVVLEIAALS
                     GKGYPAPVELDTGTGKPAKKPAAKTE"
BASE COUNT          550 a          695 c          721 g          568 t
ORIGIN      
        1 ggcagttact gctgtattca cgcgataagc tgatgcgcac ttatcgcctc ggactcggat
       61 taagtcccgt cggcgacaag gtgcggcagg gcgatcgccg cacgcccgaa ggcgagtttt
      121 acatcttcac caagaacgac aagagcgctt tctatctttc gctcggagtc agctatccaa
      181 acggcgagca tgcggcgcgc ggattacgcg ctggattgat cggcaaggcg caatacgatt
      241 ccatcatccg tgctttgcgc gcaaaaagga cgccgccgca gaacaccacg ctgggcggcg
      301 acatatacat tcacggcaac ggcgccggca gcgattggac atggggctgt gtcgcattgg
      361 agaatgaaga tattgaggag ctctaccagg caatagcggt agggactcct gtgacgataa
      421 aaccgtgacc gagatcaaac ccttcaagat tcgcgatgtg gtgatcactc caccgctaat
      481 tctgtcgccc atggccggtg tcaccgacgt gtcgtttcgc cggttgttga agcgctgtgg
      541 cggagtaggg ctgacagttt ccgaattcat ctccgttgaa gggctgaccc ggaacaatcc
      601 caagtcgaaa cggcagatgc gcttttacga agatgagcga ccctttgccg cccagatctt
      661 cggcgggcag tccgagcgaa tggtaatggc agcgcagatg gcggaggaag tgggcgcaga
      721 cattttggac atcaactgcg gttgtccggc gccaaaagtt gtgaagcacg gcggcggttc
      781 cggattgctg aaagaccaca cgcgcctgga atcgatcctt aatgagatca agaaggcgat
      841 caagatccct ctgaccatca agattcgcgc gggcttttac gaccacacta tcaacgcggt
      901 cgagacggcg aaactagccg aggcttgtgg tgtcgaacac atcgccttac acggccgcac
      961 gaaagagcaa ggctaccgcg gattggcaaa ctgggatctg gtgaaacaga tcaaggaaac
     1021 ggtaaaggtg ccggtatccg gaagcggaga cgtgacgacc gttgaagggg cgttcgcgcg
     1081 ctttcgcgaa acgggttgcg atggtgtttt gattggccgc ggggcgatgg cgaatccctg
     1141 gatcttccga caaatcgaag atgcgatgca cggccgcgag atgtttcggc caacgcttgc
     1201 cgacaagcga gcgttgttgc ttgattactt cgtcatgttg cgcgaggaca tgccagaaac
     1261 gccggctatc aatcgcatga agcaactggc gggacagttc acccgcggtc tgcaaggcgg
     1321 ggcgctgttc cgcacgtcgc tctatcactc acactcggtt gctgagattt tggagcgtat
     1381 cgaggaatac ttcgaagcga tcgaggtggg taagccttac ttcggtgaag cgggcgcccc
     1441 agttgaagaa gcgcccgagt tagattcctg cgaagctgcg agtgctgcgt gagcatcagg
     1501 cacctaaccg ccgaaacgcg aagacgcaga ggcgcaaaga aacacataac agttaagcga
     1561 gcggtttaac gaaaaattct ctttgcgcct ctgcgtcttc gtgcctctgc gcttagatcc
     1621 cgccgctatt cggttttagc tgccggtttc tttgccggct tgccagtgcc ggtatcaagc
     1681 tccactggcg ccggataacc ctttccactc agagctgcga tttccaacac aacctgtttc
     1741 tgctttctgc tgatcggctt gcgtggagtc ttcggcttct tctccccggc attcgccacc
     1801 gctgaattct cgttgccgtt gttagtgggg gtcgccgggc tcgcattcgc gttcgctgct
     1861 ttaggaactt gtttctgcgg gcgcgacgac atcgcgttca gcgcttccag aatctgatca
     1921 cgttcctccg cgctaaaatc ttcaaaccga acgttgttct tcgctaacgt cactcgcagg
     1981 ttctcttccg tattcgtagt ctgatcgtag acatccttgt agatgtagag cttgccatct
     2041 tccacgacga tagtttcata acgcagctca accggaaccg gcgcgtctag tttgagggac
     2101 cgcgtcttcg ttgagtctct aaggtaccca gcgatcgttt gatcagaaac ttcctttccc
     2161 accgccccag cgagggtttt ggcgaaatct ttaacctgcg cgttggtcag gccaacacaa
     2221 ccgtgagaag caaaggttcc gatctttgcc agtggtttgc cgccgtgaat gagtgaaggc
     2281 atgccgatcg gaatcttgat cggcccgagc ggattcaacg gactccccgc cggtaccttc
     2341 tctcccaccg caatgttcct catgtttgcc acccacggct cgtcaggcgg agtccaggtg
     2401 ggattaaaga taagcgtctg cgctttcctt agtcctgtcg gcagcgggaa ctgaggataa
     2461 ccaatgccaa tcttatacga cttgatcagg ttgccatccc gaaatacatc catgcgataa
     2521 gcgggaatgt tgac
//
LOCUS       DASUEX010000686         4881 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_564942, whole genome shotgun sequence.
ACCESSION   DASUEX010000686 DASUEX010000000
VERSION     DASUEX010000686.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4881
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_564942"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1314)
                     /locus_tag="VFV61_11415"
     CDS             complement(<1..1314)
                     /locus_tag="VFV61_11415"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX4901133.1"
                     /translation="MSVTFLPAYGQDAPLSGFDDYVNKALREWEVPGLAIAIVKDDRI
                     VMAKGYGVRKLGDPTLVNERTLFAIGSSSKAFTAASIAMLVDEGKLKWDDRVTKYLPE
                     FELYDPYVTRELTVRDLLCHRSGLERGDFLWYGSEYDRDEILRRTRYLKPTWSLRSTF
                     GYQNLMFLAAGQLIARVSGKSWDEFIRQRIFVPLSMTASNTSIRDFKNADNVSSPHAK
                     VEEKIEVIPWRNIDNIAPAGSINSNVVEMAQWVRLQLGQGEYQKQRLLSSGAAKEMQT
                     SQTVIRAEPPYSIFYPEAHFLNYGLGWFLHDYKGRKVVEHGGAIDGMRAQVAMIPEEK
                     LGLVILTNMGGSALPLPLMFRIFDAYLAAPQRDWSAEMLKTFKSFEEQGKAAQKKQEE
                     ERVKNTSASHALTEYAGTYKNDLYGDVKVAHDSGKLNLRFGPAFTG"
     gene            1633..2583
                     /locus_tag="VFV61_11420"
     CDS             1633..2583
                     /locus_tag="VFV61_11420"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEX4901134.1"
                     /translation="MNSSNSRKKKRNLSNIPSEPDAKASLLERRFAKAQAELNPRRQR
                     LLKAILDSAEETCFLSSRELAKRYRVDATTILRTTQVLGYQSYADFSADLRQHFVARI
                     TPYTALKAATREKRSVADHIDHSLEKSLDNINTLRSDLDRQRIIELAKVIKRSRRILV
                     VGIDFAASLANCLAYGLLGLGFDAGAPIGGTGNLQHKVEVLTPKDVLVAISFGQCLRD
                     AVEAALQAKKQGVPTFGITDSDTTPIARYCDAHVVASVVSPSFLNSYVAPMAVINAIH
                     VACAHIDPKRALTRLRPTDREYAAGPRWYREPKGSNGDLA"
     gene            2655..3470
                     /locus_tag="VFV61_11425"
     CDS             2655..3470
                     /locus_tag="VFV61_11425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4901135.1"
                     /translation="MREVESLQKEVWGCDDRDIVPLTILAATREVGAILVGAFDGSSL
                     IGFAYSFVGREHNRLVHHSHMLAVRATHRNLNLGYRLKLAQRERVLAQGINRMTWTFD
                     PLQSLNAYFNFAKLGVVADTYKVNFYGEATSSFLHQIGIGTDRLWVTWLLDSGRVHER
                     QQTKGQSRTSTPDRATIACLVQVGPNNVPQRGRASELTDHNDISIEIPADINALQRES
                     PQLAMRWREATRWAFSEAISSNYQIDDFCGASRHDESIGVYLLSYDKKDKSFA"
     gene            3526..4695
                     /locus_tag="VFV61_11430"
     CDS             3526..4695
                     /locus_tag="VFV61_11430"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEX4901136.1"
                     /translation="MNDLGATQLSASRAFLARRQDEIISSICKLVETESPSGDFEGSC
                     AVVDLLVDIARSIPAISSVERIPVPGYGEHVRMRAFDCQANDSHTTLVLGHTDTVHPR
                     GTLSTQSVYSDAGRLYGPGIFDMKGSCVLALEALRTLVTLNFIPKRAVVLLLTCDEEA
                     GSQTGRLLVEQEARGAAQVLVLEPPAPGGHVKTLRKGVGMWKVTAHGIASHAGLNPEA
                     GASAILELARQTDRFHAMSDAASGTNFNVGVVRGGTRSNVVPAEAEIEIDVRFSCTNE
                     AQRVDELMSTLRSFDERVQLTVKGGINRGPLERTEGVVKLYQQAREIAACLGFELGEQ
                     AVGGASDGNFVAALNVPVLDGLGINGAGAHAANEHIFISDIALRGALLAGLLATL"
BASE COUNT         1114 a         1331 c         1335 g         1101 t
ORIGIN      
        1 gccggtgaat gccgggccaa aacgcaaatt caatttgccg gaatcatgag caacctttac
       61 atcgccatac aagtcgttct tgtaagtccc tgcatactca gttagcgcat gggaagcgct
      121 ggtgttcttg acccgttcct cctcctgctt cttctgcgca gctttgcctt gctcctcgaa
      181 ggacttgaac gtcttcaaca tctccgcact ccagtcgcgc tgcggcgccg ccagatacgc
      241 gtcgaagatt cggaacatga gaggaagtgg cagggcgctg ccgcccatgt tagtgaggat
      301 tacaagcccc agtttctcct cgggaatcat cgctacctgg gccctcattc catcaatcgc
      361 gccaccgtgc tcaacgacct tacggccttt gtaatcgtgc agaaaccacc ccagcccgta
      421 gttcaaaaaa tgagcttcgg gatagaaaat cgaatacgga ggttcggcgc ggatcacggt
      481 ctgtgaggtt tgcatctctt tagcagcgcc tgaactcagc aggcgctgct tttgatactc
      541 tccctgtccg agttgcaacc ggacccactg ggccatctcc acgacatttg aattgattga
      601 gcctgcgggc gcgatgttgt cgatgttgcg ccacggaatt acttcgattt tttcttcaac
      661 tttagcgtga ggcgacgata cattatcggc gtttttgaag tctctgatgc tagtgttgct
      721 cgcggtcatg ctcagtggaa caaagatccg ttgtcggatg aactcatccc agctctttcc
      781 gcttactctg gcgatgagtt gtccggctgc caggaacatg aggttctggt agccgaatgt
      841 acttcgtaga ctccaggtgg gcttcaagta gcgagtgcgg cgcagaattt cgtcgcggtc
      901 gtattcgctg ccataccaaa gaaagtcgcc gcgttcgagt ccgctacggt gacacagcag
      961 atcgcgcact gtcagttccc tggtaacgta aggatcgtag agctcaaact ccggcagata
     1021 tttcgtgacc cgatcgtccc acttcagctt gccttcatcc acaagcatcg caatcgacgc
     1081 ggcggtgaat gctttcgagg atgagccaat ggcgaaaagc gtgcgctcgt tgaccagcgt
     1141 agggtcaccg agctttctta ccccgtaacc ttttgccatc acgatccgat cgtcttttac
     1201 aatcgcgatc gccagcccgg gcacttccca ttcacggagc gccttgttga cgtagtcatc
     1261 aaatccactg agcggcgcat cctgaccgta ggccggcagg aaggtgaccg acacaaaaag
     1321 cagaaagata attaaacgaa agcaccgtcg gtgggccata ggatttctcg attaaacggg
     1381 gagcgctgcc ttgaacggac accatataca gcaggtgctg cacaatgtca ataatgccgc
     1441 agatgctgca tagtgtcttg tctgctgaac agtgatttgc tatctttccg tcgcgaaccc
     1501 ttcctcacga gtcatataat ggcggacgct gacgacgtgg catagaagtc gaaaactgaa
     1561 ctttcagatt ggcttagggt gtcctgctgg cagcaaacaa agtaaacaca ggcccaggtt
     1621 ggagagccga agatgaacag ctcaaactca cgcaagaaga aacggaacct ttccaatatt
     1681 cctagcgaac ctgacgcgaa ggcttcatta cttgagaggc gttttgccaa ggcgcaagca
     1741 gagttaaacc cgcgtcgcca acgcttgctc aaagcgatcc ttgactcggc ggaagaaact
     1801 tgtttcctgt cctctcgtga actagctaaa cgctatcgcg tcgatgcgac gacgatactc
     1861 cgaaccactc aggtgctggg ttaccagagt tatgctgact tctcagctga cctgcgacag
     1921 cactttgtgg ctcgcatcac gccttatacg gctttgaagg ccgccacccg ggagaagcga
     1981 agtgtcgctg atcacatcga tcactctctg gagaaatcac tagacaacat aaacaccttg
     2041 agatcggacc tggatagaca gcgcatcata gaattggcca aggtgatcaa gcgctcacgt
     2101 cgcattctcg ttgttggcat agacttcgcc gcttcgctgg ccaattgcct ggcttacggg
     2161 ttgctgggac tcgggttcga tgccggagct ccgattggcg gcaccgggaa tctgcaacat
     2221 aaggtcgaag tcttgacacc gaaagatgtg ctcgtggcca tcagctttgg tcaatgtctc
     2281 agggatgcgg tcgaggcggc gctacaggct aagaagcaag gcgtcccgac tttcggaatt
     2341 accgacagcg atacaactcc gattgcacgc tactgtgacg ctcatgtggt tgcttctgtc
     2401 gtcagcccct cgtttctcaa ttcttacgta gctcccatgg ctgtgataaa cgccatccac
     2461 gtagcttgcg ctcatattga tccgaagcgg gcgcttaccc ggttaaggcc gacggatagg
     2521 gaatacgctg ctggcccacg ctggtaccgc gagcccaaag ggtctaacgg tgatttggcc
     2581 taaagattat tattgtgtcc agcgcaagtg aagaatccag gatcattatc cgcgacatag
     2641 aactcatcgc ggagatgcgg gaggtcgaat cgctccagaa agaagtttgg gggtgtgacg
     2701 atcgcgacat tgttcctctt accatccttg ctgcgactcg ggaggtcggt gcgattctgg
     2761 tcggtgcgtt tgacgggtcg tcactgatcg gcttcgctta cagtttcgtc gggcgagaac
     2821 acaatcggct cgtccaccat tctcacatgc ttgcggtgag ggcgacccat cgcaacctca
     2881 atcttggtta ccgactcaag ttggcgcagc gggaacgagt tctcgcgcag ggaatcaacc
     2941 ggatgacatg gacattcgat ccactgcaaa gtctgaatgc gtacttcaac ttcgccaaac
     3001 tcggagtcgt cgcggacact tataaggtca acttctacgg tgaagcgaca tcgagcttcc
     3061 tgcatcagat tggtatcggc accgatcgtc tctgggttac ttggctgcta gacagcggcc
     3121 gcgtgcacga acgacaacaa acaaaaggcc agagcagaac ctctacccct gaccgcgcca
     3181 caatcgcatg tctcgttcaa gttggcccaa acaacgtgcc tcagaggggg cgggcatcgg
     3241 aactcaccga tcacaacgat atctcgatag agatcccagc ggatattaat gcgttgcaac
     3301 gtgagagccc acaattggcg atgaggtggc gggaagcaac gcgctgggcc ttcagcgagg
     3361 cgatatcttc caattatcag atcgatgact tctgcggtgc atccaggcac gacgagtcca
     3421 ttggcgtcta cctcctcagc tacgataaga aagacaaaag cttcgcttaa aaagttggtt
     3481 ggcgatcgat atccttcaag tatggagctc tgagccgagc agatcatgaa cgatctaggt
     3541 gccactcaac tgtcagcgtc acgtgctttt ctcgcgcgcc gacaggacga gatcataagc
     3601 tcgatttgca agctggtcga aaccgagtcg ccctcagggg attttgaagg tagctgcgcc
     3661 gtcgttgacc tccttgtcga tattgcccgc tccatcccgg cgatcagttc cgtcgaaagg
     3721 atccctgttc ctggctatgg cgaacatgtg cgtatgcgcg cttttgattg ccaggctaac
     3781 gattcgcata caacattggt gcttgggcac actgacacgg ttcacccgcg cggcacctta
     3841 tcgacgcaga gtgtgtactc ggacgcaggg cgactttatg gtccaggtat cttcgacatg
     3901 aagggatcct gcgtgctcgc cttggaagcg ttgcgcactt tggtaactct aaatttcatt
     3961 cccaaacgag ctgtggtctt acttctgacc tgtgatgagg aggcaggaag ccaaaccggt
     4021 cggctgcttg tggaacagga ggcacgcggc gcggcgcagg tgctggtact tgagccgccc
     4081 gcgcctggcg gtcacgtgaa aaccctccgc aagggtgtgg gcatgtggaa agtgacggct
     4141 cacggaattg catcgcacgc cggccttaat cccgaagcgg gggcgagtgc tattctggaa
     4201 ctcgcccgtc aaaccgatcg gtttcatgcg atgagcgacg ctgcgagcgg cacgaatttc
     4261 aatgttggcg tcgtgcgcgg cggcacgcgc agcaacgtgg tcccagcgga ggcggagatc
     4321 gaaatcgacg tccgcttcag ttgcacgaac gaagctcagc gggtcgatga attgatgagc
     4381 accttgcgat cgtttgacga acgagtgcaa ctcacggtta agggaggcat caatcgcggc
     4441 ccgcttgagc ggaccgaagg cgtagtgaaa ctttaccagc aggcaaggga gatagctgct
     4501 tgtctcggat ttgaacttgg agagcaagcg gtcggcggcg cttcggatgg caactttgtc
     4561 gccgcgctta atgttcctgt actcgacggt cttggaataa acggcgccgg cgcacacgcc
     4621 gcaaacgagc acatctttat ctccgacatt gcactgcgcg gtgctctctt ggcgggactg
     4681 ctagcgacgc tttaacaaac gatgaaagct ttcaaagttg agagacagaa aaagtagaac
     4741 cggcctgcgc tgatgaaacc aatgaagacg aaatcccgac ctgatattcg agcgcttaca
     4801 cgactcgtgc cctccagcgg caaccttgtt caaggccagt cggaactcgc tattagtccg
     4861 gctttggctg aggcagctag t
//
LOCUS       DASUEX010000687         3211 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_220696, whole genome shotgun sequence.
ACCESSION   DASUEX010000687 DASUEX010000000
VERSION     DASUEX010000687.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3211
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220696"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..2560
                     /locus_tag="VFV61_11435"
     CDS             101..2560
                     /locus_tag="VFV61_11435"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX4901137.1"
                     /translation="MTLHCRRRFQSQTTLVLTSLFLVLLALPTKAQEGYRKAPPEVSS
                     ILSAPVTPNIYLSPSRDAVLFATSLRYPPLADLAEPMLRLAGQRINPNTNSQHRYPYF
                     VALSLKRLTDDGEIKIALPPGAKIGAPIWNADGKRFVFTNTTANGVEVWSGEASNGAV
                     KKLKDVRLNTIGGDALQWMPDNRTLMVLLVPAKRAAAPTPVNVPREPNWQESTGRPGP
                     VRTFQDLLKTPHDEDLFEYYATAQLALVDSASGKISGVGAPGIFRSIDVAPDGNHILV
                     GRIHRPFSYLYPDFAFPRDVEVWDTKGKLIHKVASLPLADQIPIDGVATGPRNYRWRP
                     TEPATLIWVEALDGGDPKKKVSHRDRVLMLKAPFNAAPTELYKTEQRFASMQFGEKAG
                     LVFISDYDRDRRWTRTFMMNLDRNETPTMIWSRNVQDRYGDPGTPVTRLLHGNMRAIV
                     QDGDWILLNGAGASPEGDRPFLDRFNLTTLKSERVFRSEGNNYEAVVGILSDDGKRFI
                     TRRESSTETPNYQLVDARSSAVLVKSLTKFPDPTPQLSGIKKQLVTYNRADGVQCSFT
                     LYLPPNYKAGTRLPTVVWAYPLEFTDASTAGQVTGSTQRYTQLIGPTHLFFLLQGYAV
                     LDNATMPVVGDPETVNNNYIEQIVMSAKAAIDKAAEMGVTDPERVGVGGHSYGAFMTA
                     NLLAHSNLFRAGIARSGAYNRTLTPFGFQSERRTIWEAPDLYIKVSPFMVANKINEPI
                     LFIHGEADDNTGTFPIQSERMYQAVRGNGGTVRLVTLPLEAHGYASRETIEHVLYEMI
                     TWFDKHVKNAPPRTKEQTATN"
     gene            complement(2788..>3211)
                     /locus_tag="VFV61_11440"
     CDS             complement(2788..>3211)
                     /locus_tag="VFV61_11440"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX4901138.1"
                     /translation="TRKEVAGKRYLNKDVYVLTSKYTFSGAEEFTYNLKNLKRATIIG
                     ETTGGGAHPGAGFRLNEHFSMFVPTGRAISPITKTNWEGTGVKPDIEVPAEQALTVAR
                     LMALKKSLPTLTNPGFKEGVQGEIEKLEKEMNELKAKN"
BASE COUNT          729 a          867 c          888 g          727 t
ORIGIN      
        1 agaaatccat tgacatccac taagccgttt cataagtttt agaacttccg ctcgtcgcga
       61 ttcaagtgta atcctactaa cccacgaccc aggagcgatt atgactctcc attgccgccg
      121 caggtttcag tcccaaacca cgcttgttct aaccagcctt tttctggtgt tactcgctct
      181 gccaactaaa gcgcaggagg ggtaccggaa agcgccgccg gaagtgtcca gcatcttgtc
      241 ggctccggtg actccaaata tttatctcag tccgtcgcgt gacgccgtgt tgtttgcaac
      301 gagcctccgt tacccaccgt tggcagattt ggcggagccg atgctgcgtt tggcgggcca
      361 gcgcatcaac cccaacacta acagccagca tcgttaccct tatttcgtcg cccttagcct
      421 gaaacgtctc acggacgatg gtgagatcaa aatcgctttg ccgccgggtg ccaagattgg
      481 tgcgccaatc tggaacgccg acggcaagcg ctttgtcttt accaacacga ccgcgaatgg
      541 tgttgaggtg tggagtggcg aagccagcaa cggagccgtt aagaaactaa aagacgtacg
      601 cttgaataca atcggcggcg acgcgttaca gtggatgccg gacaaccgca ccttaatggt
      661 cttgctggtg ccggcaaaga gagctgcagc gccgacgccg gtgaatgtac cgcgcgagcc
      721 gaactggcag gagagtacgg gccggcctgg tcccgtgcgc acgtttcagg atctcctcaa
      781 gacgccgcac gatgaagatc tttttgagta ctacgcgaca gcccagctag ctctggtaga
      841 tagcgcgagc ggaaagatat cgggagtggg cgctcctggc atcttccgtt cgatcgatgt
      901 cgcaccagat ggcaaccata tcctcgtagg acggattcat cgtcctttct catatctcta
      961 tcccgacttc gctttccccc gcgacgtcga ggtttgggac acgaaaggga agttgattca
     1021 taaggtagcc agcctgccgc ttgcagatca gattcccatt gacggcgtcg ctaccggccc
     1081 gcgcaattat cgctggcggc ccactgagcc tgcgactctg atttgggtgg aagcgctcga
     1141 tggtggtgat ccaaaaaaga aagtgtcgca ccgcgatcgc gtgctgatgc tgaaagcgcc
     1201 attcaacgcc gcgcccacag aactctacaa gaccgagcag cggttcgcgt caatgcaatt
     1261 cggcgaaaag gccggccttg tgttcatctc cgattacgat cgcgatcggc gttggacgcg
     1321 caccttcatg atgaacctcg accgcaatga aactccaacg atgatttgga gtcggaacgt
     1381 tcaggaccgt tacggcgatc ccgggacacc ggtaacacgt ttgctccacg gcaacatgcg
     1441 agcgatcgtg caggacggtg attggatcct tttgaacggc gcgggcgcct cgcctgaggg
     1501 tgaccggccg tttctcgatc gatttaatct gaccacgttg aaatcggaac gcgtctttcg
     1561 gagtgaagga aataattacg aagccgtcgt tgggatcctg agcgacgatg gcaagagatt
     1621 cattacgcgc cgcgaaagct cgacagagac gcccaactac cagctcgtcg acgcgcgctc
     1681 aagtgcggtg ttagtaaagt cgctgacaaa atttcccgat cccacaccgc agttgagtgg
     1741 tatcaagaaa caacttgtta cttacaaccg cgctgacgga gtgcagtgtt cgtttacttt
     1801 atatttgcca ccgaactata aggctggtac ccgtttgccc acggttgtct gggcctatcc
     1861 gcttgagttc actgatgcat caacggcagg acaagtgaca ggatccacgc agcgctacac
     1921 gcaactgatt ggacccacgc atctgttttt cctcttgcag ggttatgctg tgttggataa
     1981 cgccacgatg cctgtggttg gtgatccgga aacggtcaac aacaactaca tcgaacagat
     2041 cgtgatgagc gcaaaggcgg cgatcgataa ggccgcggaa atgggagtga ctgatcccga
     2101 acgggtgggc gtgggcggtc acagttacgg cgcgtttatg acggcgaatc tgctggcgca
     2161 ttcgaacctg tttcgcgcgg gaattgcgcg cagtggtgcc tacaatcgca ctttgacgcc
     2221 tttcggtttt cagagtgagc ggcggacgat ttgggaggcg ccggatctat atataaaggt
     2281 ctcgcccttc atggttgcca ataagatcaa tgagccgatt ctgtttattc acggagaagc
     2341 ggacgataac acgggtacgt ttccgatcca gtccgaaaga atgtaccagg cagtgcgggg
     2401 caatggcggc acggtacgac tcgtgacgct gccgttagaa gcgcacggtt acgcgagccg
     2461 ggaaacgatc gaacatgtgc tctatgaaat gattacctgg ttcgacaagc acgtgaagaa
     2521 cgcgccgccg aggacgaagg aacaaacagc gacgaattag tcgagaatct gactgattcc
     2581 gaaagccgtc cttttggatc agcattaagc caaggcgcca agacgcagag gcaaagacgc
     2641 agggacgcag agacgccaag gatcgctaaa cattggagtt gaaccgtacg cttattcaac
     2701 tctgcacgtt tgaggttctt ggcgtctctg cgtctttggc ggtagttagc gttcggtaaa
     2761 acaagatccg aatagctcgc cagaccccta gttcttcgcc tttagttcgt tcatctcctt
     2821 ttccaacttt tcgatttcac cctgcacgcc ctctttgaaa ccggggttgg ttagagtggg
     2881 gagagatttc ttcagtgcca tcaggcgggc gacggtcaac gcttgttccg ccggcacttc
     2941 aatgtctggc ttcactcctg ttccttccca gttggtctta gtaatgggac tgattgcgcg
     3001 accggtcggc acgaacatac tgaagtgctc attaagacgg aaccctgcgc caggatgtgc
     3061 gcctcctcca gtagtctcac caataatggt agcgcgcttc aggttcttga ggttgtaagt
     3121 gaattcctca gctccagaga aagtgtattt gctggtcagg acatagacgt ccttgttcag
     3181 gtaacgtttg ccagcaactt ccttgcgcgt c
//
LOCUS       DASUEX010000688         3944 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_221710, whole genome shotgun sequence.
ACCESSION   DASUEX010000688 DASUEX010000000
VERSION     DASUEX010000688.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3944
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221710"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..801
                     /locus_tag="VFV61_11445"
     CDS             <1..801
                     /locus_tag="VFV61_11445"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX4901139.1"
                     /translation="LVTGLRFDYFDLQFHNNRTGDNLRRIDRLVSPHAGLVVKPVMSF
                     SLYGTYSVSYLPSSGDQFSSLTTITQQVKPEKFNNYEVGVKWDVHRFLFLTAAAYRQD
                     RLNTRATDPNDPTRIVQTGSQRTDGFELGLNGNVTRSWRIAGGYAFQDAVITGATTNA
                     AAGKRVGLVPRHTFSLWNNYQFMSKLAAGLGIIHHTDSFAAVDNAVVLPGYTRADVAV
                     FYSITERWRLQGNLENLFNANYYVNADGNNNISPGRPRSVRFGLIARF"
     gene            947..1318
                     /locus_tag="VFV61_11450"
     CDS             947..1318
                     /locus_tag="VFV61_11450"
                     /inference="COORDINATES: protein motif:HMM:NF044994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HEX4901140.1"
                     /translation="MRPEQLAHFKEILLMQLREHTNNVRDDQAAALELSDDGVKDSMD
                     LSVMDVNKELALRLGDRESQMVADIDQALLRIKEGSYGVCARCNKPIDERRLEAMPTA
                     RYDADCQTRLEANRSTERPSL"
     gene            complement(1453..2373)
                     /gene="xerC"
                     /locus_tag="VFV61_11455"
     CDS             complement(1453..2373)
                     /gene="xerC"
                     /locus_tag="VFV61_11455"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEX4901141.1"
                     /translation="MEQLLTQFLEHLRYERNVSEHTLRNYASDLNQFLDHLAPAHPQT
                     RKRTPVDLAQIDHITIREWLSTLHSAQKKKSSVARKLAALRTFFQFAVREGLLELNPA
                     KLVSTPRLEKRLPQHLSIEEAIKFIETPDVETDLGKRDRAMLELMYATGVRVSELTKM
                     GLADVDFKNRLIRVTGKRRKERIVPFGEEAQEALQKYLAVRDRFLNSAPITEREPDAL
                     FLNYQGTRITTRSVGRMVEKYIRICAGMHNISPHALRHSFATHLLDSGADLRDIQELL
                     GHARLSSTQIYTHVSMEKLIEVYDKAHPKA"
     gene            complement(2396..3481)
                     /locus_tag="VFV61_11460"
     CDS             complement(2396..3481)
                     /locus_tag="VFV61_11460"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX4901142.1"
                     /translation="MNSSARGAWGVLVATVSLVLLLFLWDANVTAQSGRRKDTKPKAR
                     PAPSPIAEANRPASSQTPVSTSSPTTPGEDVEESDIVRVSSNLVPIPASVVDNKGTAL
                     TTLKVDDFELRVDGQVRPISDISRSDSPVRLAMLFDNSGSLDMGRAFEKHAAQQFFRR
                     VLRPSDEAAIYSVESESYLAQPMTRDIRRLEQTIELFGKPEGSTSLFDTIIAAAAYLR
                     PYSGRRVIVIVSDGIETTSRADFETTLHHVLADDCQIFVVQTGLYDGVNLRALAAERR
                     MEQLAAQTGGAAYIPKTTSALDFAFQEIAADLAQQYVLSYYPGAENRDGRFHVIELRI
                     KSRKDVRVRARRGYYSPKAANVAAGGW"
     gene            complement(3478..>3944)
                     /locus_tag="VFV61_11465"
     CDS             complement(3478..>3944)
                     /locus_tag="VFV61_11465"
                     /inference="COORDINATES: protein motif:HMM:NF013314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX4901143.1"
                     /translation="GLERAEFLQFGQIHRNTYICSPRVLSETMQMRERPNVFFAGQIT
                     GVEGYVESVAMGWLAGQNAARLAGNHELITAPGRSAIGALARYVTNAESKHFQPVNIT
                     FALLQPLEEQDRRRLRKKRDRHQFQVELALTEWDAWLKDLQLELATMPARAS"
BASE COUNT          893 a         1053 c         1067 g          931 t
ORIGIN      
        1 ttggtgaccg gtctgcgttt tgattacttc gatttgcagt tccacaacaa tcgaactgga
       61 gataacctgc gacgaattga tcgtttggtg tcgcctcatg cgggcttggt ggttaagccg
      121 gtcatgagct tctcgcttta cggaacgtat agcgtttcct atttgccgag ctcaggagat
      181 cagttctctt ccctaacaac gatcacgcag caagtcaaac cggaaaagtt taataactac
      241 gaagttggtg tgaaatggga tgtacatcgc ttcctgttct taaccgcagc tgcctatcga
      301 caggatcgcc tcaatactcg ggcaactgac ccgaacgacc caactagaat tgtgcaaaca
      361 ggtagtcaga gaacggacgg gtttgagctt ggtcttaacg gaaatgtgac acgatcgtgg
      421 cggatcgctg gtggttatgc cttccaggac gcggtgatca ccggtgccac gacaaacgct
      481 gcggccggca aacgagtggg cttagtgccg cgtcacacat tctcgctctg gaataactac
      541 cagttcatgt ccaagctagc cgcggggctc ggaatcattc atcacactga ttcgttcgcg
      601 gcggtcgaca acgcggtcgt gcttcccggt tataccagag cagacgttgc cgttttctat
      661 tcaattacgg agcgctggcg cttgcagggt aatctggaga acctgttcaa tgctaactat
      721 tacgtcaatg cggacggcaa caacaacatc tctccaggtc ggcctcgtag cgttcgcttc
      781 ggtttgatcg cacgcttcta aagaggcttg atcttttggt tgctgaatcg ctgcattgct
      841 cgagattaaa gggtcaatct cagagcgcgg ttacgcgaag gtaagagcta cgctataatg
      901 cgggctcaac tctttgagtt taaatcggta gacaggtggt gaattcatgc gtccggaaca
      961 actcgcacac tttaaagaga tccttctcat gcaactgcgc gagcacacga ataatgttcg
     1021 tgacgatcag gcagcggcac tggagttgtc cgatgatggt gtcaaggaca gcatggatct
     1081 ctcggtgatg gacgttaata aggaacttgc gctccgcctg ggtgatcgcg agtcgcaaat
     1141 ggtggccgac attgatcagg cgctgctgag aatcaaggaa ggaagttacg gcgtctgcgc
     1201 tcgttgtaat aagccaatcg atgaacgacg tctcgaagct atgcccaccg ctcgttatga
     1261 tgccgactgc caaacccgcc ttgaggccaa ccgcagtacc gagagaccgt ccctctaaga
     1321 ctggtcgcaa gtcaggtttc aggtttcaga cttgggattc aagctggttt taactcaatc
     1381 ccgattaaag cctgagaccc tatccctgaa acttgaaacg tcaaacctga aacggagaga
     1441 cttgagactt attcaggcct tcggatgcgc tttgtcgtag acctcgatca gcttttccat
     1501 ggaaacgtgc gtgtagattt gggtagagga gagacgcgcg tgccccagta gttcctggat
     1561 gtcgcgcaag tcggccccgc tgtccagcag atgagtagca aacgaatgcc ggagagcgtg
     1621 cggactgatg ttatgcatgc cggcgcaaat gcgaatgtac ttttccacca tgcggccgac
     1681 cgaacgagtg gtgatcctgg ttccctgata gttgagaaag agcgcgtcgg gttcgcgttc
     1741 ggtgatgggc gcgctgttca agaacctgtc tcgtacggct aaatacttct gcaacgcctc
     1801 ctgcgcctcc tcgccaaaag gaacgatgcg ttccttccgg cgctttccgg taacccggat
     1861 tagtcggttc ttgaagtcga cgtccgccaa acccattttt gttaactcgg aaactcgcac
     1921 acccgtggcg tacatcaatt ccaacattgc ccggtcgcgt ttcccgagat cagtttcgac
     1981 atcaggagtt tcgataaact tgatcgcttc ttcaattgag agatgctgcg gcagtctttt
     2041 ctcgaggcgc ggcgtggaaa ccagcttggc gggattaagc tccagcaagc cttcgcgcac
     2101 agcaaactga aaaaacgttc gcaaggcagc cagtttgcgc gctaccgacg acttcttttt
     2161 ctgcgctgaa tgcagggtgg aaagccactc acgaatcgtg atgtgatcta tctgggcaag
     2221 atcaaccggc gttcgtttcc gggtttgggg atgggccggc gcgaggtgat ccagaaactg
     2281 attgaggtcg ctggcatagt ttcgcagcgt atgctcactg acgttgcgct catagcgcag
     2341 gtgttcgaga aactgtgtta gcagttgctc catagcttcg caacttaggc gctagttacc
     2401 aaccgccggc agccacattc gcagctttcg gtgaatagta gcctctgcgc gcacgcactc
     2461 gcacatcttt gcgtgatttg attcgcaatt caatcacgtg gaaacgtcca tcgcgattct
     2521 ccgcgccggg gtagtaactc aacacatatt gctgcgcgag atctgcggca atttcctgaa
     2581 aggcgaaatc gagggccgaa gtcgtcttgg ggatgtacgc ggccccgcca gtttgcgcgg
     2641 ctaactgttc catccgtcgc tcggctgcga gagcgcgcaa gttgacgccg tcatacaagc
     2701 cagtctgcac tacaaaaatc tgacaatcgt cggcgagcac gtgatgcaac gtggtctcaa
     2761 aatccgcgcg gctcgttgtt tcaatcccgt cagacacaat cacaatcaca cgccgcccgg
     2821 aatatgggcg caaatacgca gccgcggcaa tgatggtatc gaacagcgag gttgagccct
     2881 cgggttttcc gaagagctca atcgtctgct ccaatcttct tatatctctc gtcatcggtt
     2941 gcgcaagata actttccgac tcaaccgagt aaatggcggc ttcatcgctg ggacgcaaga
     3001 cacgacgaaa aaactgttgt gctgcgtgct tctcgaacgc tcgccccata tcaagactgc
     3061 cactattgtc gaatagcatt gccagtctca ccggagagtc tgagcggctg atatcgctga
     3121 ttggtctcac ctgtccatcg actcgtaact caaaatcgtc aaccttcaaa gtggtgaggg
     3181 cagtcccctt gttatcaacg actgaggcag ggatgggaac aaggttggac gacacacgaa
     3241 cgatgtcaga ctcttcaaca tcctcaccgg gagtcgtggg tgaggaagta gagaccggcg
     3301 tttgtgaact ggcaggtcgg ttcgcctcag ctatcggact cggcgctggc cgggcttttg
     3361 gtttggtgtc cttacgccgt cctgactgcg ccgtcacgtt ggcgtcccac aggaagagga
     3421 gtagcaccaa cgagaccgtt gctactaaaa ccccccaggc gccgcgcgcc gaagagttca
     3481 tgacgctctc gccggcatcg ttgccagttc tagctgcaaa tcttttagcc aagcgtccca
     3541 ttctgttaac gctaactcca cctggaattg atgacggtca cgtttcttac gaaggcggcg
     3601 gcgatcttgt tcttctaacg gttgtagcag agcaaaagtg atattgacag gctgaaagtg
     3661 tttgctctca gcattggtca cgtaacgtgc aagagcaccg atcgcgctgc ggccaggagc
     3721 ggtaatcaac tcgtggttgc cagcgagccg cgctgcgttt tgccctgcca gccaacccat
     3781 ggcaactgac tcgacatatc cttcgacccc ggtgatctgc ccggcaaaga agacgtttgg
     3841 cctttctcgc atctgcatcg tctcgcttag cactcgcggc gaacagatgt aagtgttgcg
     3901 atgaatttgg ccaaactgta agaactcggc gcgctcaagt ccgg
//
LOCUS       DASUEX010000689         2868 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_567593, whole genome shotgun sequence.
ACCESSION   DASUEX010000689 DASUEX010000000
VERSION     DASUEX010000689.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_567593"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1590
                     /locus_tag="VFV61_11470"
     CDS             <1..1590
                     /locus_tag="VFV61_11470"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HEX4901144.1"
                     /translation="CSGIYETKNAGENWRKVQGIPSQSRRTRAILQHPSIPGVVFAGT
                     TEGFWRSEKGGDADSWMVTTSRQLEINSITVHPTRPETVFIGTNNYGVMVSNDSGKTF
                     IPTNTGYSGRFANSIVADWEMPNRIYAATINTATGGGFFFTSTDNGQTWRPSMRNMPS
                     RLITYSILQDLRDANIIYLGTNLGVYRSVDRGATWAPIWAGNAAPDAKKTVAKAKKGS
                     RAQSAKTQAPRKPNDIVLRAQNALNSAGYKIAVADGRSGTRTTLAIRKFQTDKSLPVT
                     GKLDAITLAALGVAAEGAKDGQDAAVIITDGIRALVHSIDAATGTPVMLAATNVGIYR
                     TSDPLKGWQRLSFDADFDPTCIATDPQHPEQIWVGTAASGVVVSRDGGQTWQQVKDIP
                     SDVPVNTIVQDPKLRERIYVGTKQAFYASYDGGVTWNRRGGNLPFGDFTSILINSHNT
                     DEVFVGNAFQNGEIGGGVWRTLNAGKTWARIDPKGQRLPSQRIWSLAFDGQDQNVLFV
                     GSHSAGVYVVPRSETASAGSR"
     gene            1966..>2868
                     /locus_tag="VFV61_11475"
     CDS             1966..>2868
                     /locus_tag="VFV61_11475"
                     /inference="COORDINATES: protein motif:HMM:NF012784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEX4901145.1"
                     /translation="MLSDESKPNDGETSETPSGAPEAASQPAAAAAASEARTDVVSEA
                     ATGETPASEASASEAAKAASAPAPASEADDDDDAPRGDMDFGKLLDQFEQEQATLQEG
                     EVVRGTVVGISERGVVIDFGYKSEGLVNPAEFTENGVISVKPGDEVEVLVKSMETADG
                     LPILSRADAVRMKAWDDLEKAYQDGTTVKGRVMERIKGGLRIDIDGIGAFLPGSQVDV
                     RPVRNLDSLRNQEIEAKVIKLNRKRSNVVLSRKAVIENENAGRKDQTLQHIEEEIIVE
                     GQIKNLTDYGAFVDLGGVDGLLHVT"
BASE COUNT          698 a          721 c          840 g          609 t
ORIGIN      
        1 tgcagtggaa tttacgaaac gaagaacgcg ggtgagaatt ggcggaaggt gcaggggatt
       61 ccgtctcagt ctcggcgcac acgggctatt ttgcaacatc cttcaattcc gggagtcgtg
      121 ttcgcgggaa caacagaagg attctggcgg tccgagaaag gcggcgacgc tgattcatgg
      181 atggtgacta cgtcacggca actggaaata aattcgatta ccgtgcaccc gactcgtccc
      241 gagacagtct ttatcggcac caacaactac ggcgtgatgg tctcaaatga cagcggtaag
      301 actttcatcc caaccaatac cggttacagc ggcaggttcg ccaatagcat cgtcgctgac
      361 tgggagatgc cgaatcgaat atatgcggcg acgattaaca ctgctacagg tggcggtttc
      421 tttttcacga gcaccgacaa tggtcaaacg tggcgccctt cgatgcgcaa catgccgtcg
      481 cgtttgatca cttattcgat tcttcaagat ctacgcgatg ccaacatcat ttacctcggt
      541 accaatcttg gcgtttatcg ctcggtggat cggggtgcca cctgggctcc gatctgggca
      601 ggcaatgccg ctcctgatgc gaaaaagact gtggccaaag ctaaaaaggg cagtcgagct
      661 cagagcgcaa aaacccaagc tccgcgcaaa ccaaacgata tagtgttgcg tgctcagaac
      721 gcgcttaaca gtgctggtta caagattgca gttgccgatg gtaggtctgg taccaggacc
      781 acgctcgcaa tcaggaagtt ccagaccgac aagtctctgc cggtcacagg taagttggat
      841 gcgatcacgc tggcggcgtt aggggttgcg gcagaagggg caaaggacgg gcaagatgca
      901 gcagtgataa ttactgatgg catccgagcg ctcgttcaca gcatagatgc ggcgaccggc
      961 actccggtga tgctggctgc taccaatgtc gggatctatc gcactagtga tccactaaaa
     1021 ggatggcaac gtttgtcgtt tgacgccgac ttcgatccta cttgcatagc aacagacccg
     1081 cagcatcccg agcaaatctg ggtagggacg gcagcatcgg gtgtggttgt atctcgcgat
     1141 ggtgggcaga cgtggcagca agttaaagat attccgagcg atgtgccggt caatactatt
     1201 gtgcaagatc caaaacttcg cgagcgtatc tatgtcggca ccaagcaggc attctatgcc
     1261 tcatacgatg gcggggtgac ttggaatcgt cgtggtggaa atcttccgtt cggcgacttc
     1321 accagcatcc tgatcaattc tcataacact gacgaggttt tcgtcggtaa tgcctttcaa
     1381 aacggcgaga ttggcggcgg tgtgtggcgt actctcaatg cgggaaagac ctgggcccga
     1441 attgatccca agggtcaacg gttgccaagt cagagaatct ggtcgttagc gttcgatggc
     1501 caggaccaga atgtcctgtt tgtgggttca cactcggcag gcgtttatgt ggtcccccga
     1561 agtgaaacgg cgtcagccgg cagtcgttga attagcattc gaggctgcgc ttgtgtgccc
     1621 gtaactcttt gctctagtgg tctttcttta ttagccggtg gaacttagcg ccttgacatg
     1681 gtcttatttt ggcgtgtaca ttgccgacct acccggtaaa gcggcaatga tggtcggatc
     1741 aagcgggtga ctacgggcgc ggcggaaatc gaaacaacat aataacctcc ttttccaatc
     1801 tgaacggctc gcattcctcg cttcaaaatc cgacaaatcg tgaaggttac taaggcagcc
     1861 gcttgagtgt tgtccgccgc tcttcatgtg cgacatcagt actcaatgta gcgtgaaaag
     1921 atttgctaca cgcaaaaaat tgtccggggc ctattaggag tctgcatgct tagtgatgaa
     1981 tcgaagccga acgacggcga aaccagcgaa accccttccg gcgcgcctga agcagcgtcg
     2041 caaccagcag cggcggccgc cgcaagtgaa gcgcgaacag acgtagtcag cgaagcagcg
     2101 acaggggaga cgccggcaag cgaagcttct gcgagcgaag cggcgaaagc ggcatcggca
     2161 ccggcgcccg cgagtgaagc cgacgacgac gatgatgcgc cacgcggcga catggatttc
     2221 ggcaaactcc tcgatcagtt tgagcaagag caagcgaccc ttcaggaagg cgaagttgtc
     2281 cgcggcaccg tagtaggaat tagtgaacgc ggggtggtta ttgattttgg ctacaagtca
     2341 gaaggactgg tcaacccggc cgagttcact gaaaacggcg tcattagtgt gaagcctgga
     2401 gatgaagtgg aagtgctcgt aaagagcatg gaaactgcgg atggcttgcc gattctctca
     2461 cgtgccgacg ccgttcgcat gaaggcctgg gacgacctgg aaaaggcgta ccaggatggc
     2521 accacggtta agggtcgcgt aatggagcgg attaagggtg gcttgcgcat cgacatcgat
     2581 ggcatcggcg cattccttcc cggctcacag gtggatgtgc gaccggttcg caatctcgat
     2641 agcctgcgca atcaggagat cgaagcaaag gtcatcaagc ttaatcgcaa acgttccaat
     2701 gtagtgctgt ctcgcaaggc cgtgatcgaa aacgagaacg cgggcagaaa agatcaaacc
     2761 ttgcagcaca tcgaggaaga gatcattgtc gaaggacaga tcaagaacct caccgactac
     2821 ggtgcctttg tggatctcgg cggcgtagat ggcttgcttc atgtgacc
//
LOCUS       DASUEX010000690         3312 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_223443, whole genome shotgun sequence.
ACCESSION   DASUEX010000690 DASUEX010000000
VERSION     DASUEX010000690.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3312
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223443"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2720
                     /gene="bamA"
                     /locus_tag="VFV61_11480"
     CDS             <1..2720
                     /gene="bamA"
                     /locus_tag="VFV61_11480"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HEX4901146.1"
                     /translation="VPESDVLKAFRERRVGVSKESIYDPVKVRNAIRVLKELLAEHGH
                     PNATIEERRDEVSATSTAVTFDIDEGDRVRVVEIQFEGNTVFSDKSLRNSMKYVKEAG
                     LITRFKGQDILHREKLEVDLRNVDNHMRSKGYLQARHGEPRVESVGPRRTGFPILPLP
                     FLSSVDEGLRVTVPIVEGKVYRIGEMKIEGNSIFSEDQIRSVIGLNKGDMANAEKISK
                     GLFENLKKIYGSQGFIEYTAEPVPTFKENPQNANEGIVDFTITIEEGKQFTLRRLEFS
                     GNTFTRDSVLRREVLINEGDIYNQGYWEYSVIKLNQSGYFEPIDKDKDADFRTSEEEA
                     TVDINLKVTERGRQQISFNGGLSGIGGSFFGLEYSTNNLLGRGEILSINLAAGNRQRS
                     FQFSFTEPYIRNRPITAGFSVFAFSQKFFGEGTFLSQNLAAQQGASGSALDFLTTSDE
                     NLFTRNSYGGSIFASAPLSEFYRKRRFTQFSRIGASYQFSTSSVTDPKVNLEGNPDQF
                     IPVVYEQANIITSRGTVTFSYDTRNASVDPTSGREVSVGVALAGLGGDVRTYQPTVSY
                     TQFFPVRRKKSERPEVFGFRILAGTVGSFAISNKVRNSQSLAFVDGVPIFERFFLGDE
                     FTIRGYNVRSISPIAPLDTFITSQNVKVAQNAFGTPIQIPNVPTSLNVIGTFTGIGGN
                     NTAQLPRSFTPIGGDTQLLGNFEYRIPLIGNTVGLAAFADVGTSFNLRTKSDQVFSSK
                     FLPDDPFLQTVGFIPCTRLTSGFAPTTLSTLAACNANTQLALTPNLGLVMRDNRMVTT
                     QELDNARNIGPFDPLTGLPYGFQQVFLRGDTQTNTAVRLSESLFDKIGDFRSSLGMEV
                     RVQVPVLNVPFRLIFAYNPNARPNQIIQGFPFGFNEKKKVIRFSVGRTF"
     gene            2811..>3312
                     /locus_tag="VFV61_11485"
     CDS             2811..>3312
                     /locus_tag="VFV61_11485"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEX4901147.1"
                     /translation="MKTDRLIAAFAFIVALGISPVLAQRPAAGQPATAPQTAANVPDS
                     KIAVIYSDAFLDDKTGIARFTTLLNTLNREFQPRQTELQQIQQRATALNDEIEKTKSV
                     ADPKTIAAKIDQLENYKIELKRKSEDAQQAYDKRRKEIFTPLQDSIGKALEAFAKQRG
                     ITVLIDG"
BASE COUNT          811 a          912 c          848 g          741 t
ORIGIN      
        1 gcgtgccgga gtcggacgta cttaaagcct ttcgcgaacg acgcgtgggc gtgtccaaag
       61 aaagcatcta tgaccctgta aaagtcagaa atgcgattcg ggtgttaaaa gagctgctcg
      121 cggaacacgg acatccgaac gccaccatcg aggaacgcag ggatgaagtt tccgccactt
      181 caacggccgt aactttcgac atcgatgaag gtgaccgggt gcgcgtcgtt gaaatccagt
      241 ttgaaggcaa cacggtattc agcgacaaga gtctgcgcaa ctccatgaag tatgtgaaag
      301 aggccggctt gattacccgg ttcaagggcc aggacatact gcaccgcgaa aagctggaag
      361 tggatctacg caacgtcgac aaccacatgc gttcgaaagg ttacctgcag gcgcgtcacg
      421 gagaaccgcg ggttgagagc gtcgggcctc gtcgcactgg ctttccgata ttgccgctgc
      481 cattcctttc ctcagtcgac gaaggactac gggtgacagt cccgattgtt gaaggaaagg
      541 tctatcgcat tggcgaaatg aagatagaag gtaactcgat tttttccgag gaccagatcc
      601 ggagcgtcat cggcctgaac aaaggcgaca tggccaatgc tgagaaaatc tcaaaaggct
      661 tgttcgagaa tttgaagaag atttacggct cgcagggttt catcgaatac acagcggagc
      721 cggtgccgac cttcaaagag aatccccaga atgccaacga gggaatcgtc gatttcacga
      781 tcaccattga agagggcaag cagtttactc tgcgtcggtt ggagttttcc ggcaatacgt
      841 tcacgcgcga cagtgtgctg cgtcgtgagg tgctgatcaa cgagggcgat atttataacc
      901 agggctactg ggaatactcc gttatcaaac tcaaccagtc aggctacttc gaacccatcg
      961 acaaggataa agatgcggat ttccgcacca gcgaagaaga agcaaccgtt gatatcaatc
     1021 tcaaggtgac tgagcgcggg cgccaacaga tatcattcaa cggcggactt tcaggcatcg
     1081 gcggttcctt ctttggcctg gagtactcga cgaacaacct gctcggccgc ggcgaaattc
     1141 tttccatcaa cttggccgct ggtaatcgtc agcgctcctt tcagttttca ttcactgagc
     1201 cgtacatcag aaaccggccc atcacagccg gtttttccgt ctttgctttc agtcagaagt
     1261 ttttcggcga gggaaccttc ctttcacaga acctcgccgc gcagcaagga gcatcaggct
     1321 cggccctcga tttcctgacg accagcgatg agaatctgtt tacgcgcaac tcgtacggtg
     1381 gctcgatctt cgccagcgcg ccgctttcgg agttttaccg caagcgccgc ttcacgcagt
     1441 tctcgcgtat tggcgcctcg taccagtttt ccacttccag cgtgactgac ccgaaggtca
     1501 acctcgaagg caatcctgac caattcattc cggtcgtgta cgagcaggca aacatcatca
     1561 ccagccgcgg cacagttaca ttctcttacg acacgcgcaa cgccagcgtc gatcccacct
     1621 ccggtcggga agtttcggtt ggcgttgcac tcgcaggtct ggggggcgac gttcgcactt
     1681 accaaccgac ggtttcctac acgcagtttt ttcccgtgcg gcgtaagaag tcggaacgac
     1741 cagaagtctt cggcttccgc attctcgccg gcacagtagg gagtttcgcg atttcgaata
     1801 aagtaaggaa ttcgcagtcg ctggctttcg ttgatggcgt gcctatcttc gaacgcttct
     1861 tcctcggaga cgagttcaca attcgtggtt ataacgttcg ctcaatcagc cctatcgcgc
     1921 cactcgatac atttatcact tcgcagaatg tgaaagtcgc gcaaaacgcc ttcggtaccc
     1981 cgatccagat acccaatgtg cctacctcgc taaatgtgat tggaaccttt actggaatcg
     2041 gtgggaacaa tacggcgcag ctgccgcgtt ccttcacccc aatcggcggt gacacccaac
     2101 tactaggcaa cttcgaatac cgcattccgt tgattggaaa cacggtaggt ctcgctgcgt
     2161 ttgccgacgt aggcacgtcg ttcaatttgc ggactaagtc cgatcaggtt ttctccagta
     2221 aattcctgcc cgacgaccca tttctgcaga cggtcggatt tattccctgc acacggctaa
     2281 cttccgggtt cgcgccgacc actctttcaa ccctcgctgc ctgcaatgcc aatacgcagt
     2341 tggcactgac cccgaatctc ggtctggtaa tgagagataa ccggatggtc acaacgcagg
     2401 aactcgacaa tgcacgaaac ataggaccct tcgacccgtt gaccggtctt ccgtacggct
     2461 tccagcaggt tttcttgcgt ggcgacactc aaaccaatac cgcagtcagg ctctcggaga
     2521 gtctttttga caagatcggc gacttccgga gcagtctggg catggaggta cgtgtccagg
     2581 tgccggtctt aaacgtgcct tttcgcttaa tctttgcgta taatcccaac gctcggccaa
     2641 accagattat tcagggcttt ccattcgggt tcaatgagaa gaagaaagtc atacgtttca
     2701 gcgtagggcg cactttttag ttaaattgag gccaaccatg gcggtcgtca caacaactaa
     2761 gacagttgaa ccgaccgccc tttcaatgta aggagcatta caacaaagtt atgaaaaccg
     2821 atcgtctaat tgccgccttt gcgttcatcg tagcgcttgg catatccccg gtcctggcgc
     2881 agcgacccgc agcaggccaa ccagccacag cgccccaaac cgcagcgaac gtacccgaca
     2941 gcaagatcgc ggtcatctat tccgatgcat ttttggatga caagaccggg attgccagat
     3001 tcactacgct gctaaacacg ctgaatcgtg aattccaacc gcgtcagact gagctgcagc
     3061 agattcaaca gagggcaact gcgctcaacg atgagattga gaaaaccaag tctgttgctg
     3121 atccgaagac gattgcggca aagatagatc agttggagaa ctacaagata gagttgaagc
     3181 gcaagagtga agatgctcaa caagcgtacg acaagcgtcg caaagagatt tttactccgc
     3241 tccaggactc tatcgggaaa gcccttgagg cttttgccaa acagcgcggc atcaccgtgt
     3301 taatcgatgg ca
//
LOCUS       DASUEX010000691         2113 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_570192, whole genome shotgun sequence.
ACCESSION   DASUEX010000691 DASUEX010000000
VERSION     DASUEX010000691.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_570192"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..555)
                     /locus_tag="VFV61_11490"
     CDS             complement(55..555)
                     /locus_tag="VFV61_11490"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HEX4901148.1"
                     /translation="MSTPIIAVAITVSDRCANGEQEDLSGAALVELLREAGAEIIAHE
                     VLSDDPEALADSLREYAGRDDVNLIITTGGTGFGPRDNTPEVTRQVIDREAPGLAEAM
                     RAASLKHTPMAMISRGVCGIYSDCLIVNLPGSPKAVRESFNVIRPVLNHAVALLAANT
                     NHEGDE"
     gene            complement(603..>2113)
                     /locus_tag="VFV61_11495"
     CDS             complement(603..>2113)
                     /locus_tag="VFV61_11495"
                     /inference="COORDINATES: protein motif:HMM:NF021942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HEX4901149.1"
                     /translation="RLVFAPEEQAAFFGGDYDNFTYPRYCLDVTFFRVYENGAPLKTQ
                     NYFKWSAAGPADGEFVLAIGSPGSTNRLLTVSQLKYQRDVGNPLQMLVWTSRREALVR
                     YGASGPEATRRALATVRSLANSIKRLTGQQEGLSNPRMMARKEEEEKALRAEVARRPE
                     LQQAVGGAWDEIAAAYGELPAYAKRSAFSTLVWSRLGQIASSLVRYSVEIGKPNDKRY
                     DEFRDSKLESLKFGLFSPAPVYPDMEEAILTAWFVEAEKTLGANDPFVRAALNGSTPA
                     AVAKAVVSGTKLGDVAVRKALFEGGAEAIARSDDPLIALARRVEPVIRELRTWNEEKI
                     VNLETVAGQKIATARFAVYGRNVYPDANSQLRISYGRVLGYEEDTTLVPYKTTFSGLY
                     DRAASFDEKVPYDLPQRFKDGRQKLDLSTPMNFVYTADTIGGNSGSPIINRKAEIVGL
                     NFDSNIQKLPNRYWYVDETEGGRAIAIHSAGIIEALRKLYGADSLVQELTAK"
BASE COUNT          476 a          616 c          557 g          464 t
ORIGIN      
        1 atcaggatgc caggagaagt gggcactggg cagaaatatc aagccctgtt gtcctcactc
       61 atcgccttca tggttggtgt tcgcggccag gagcgccaca gcatgattaa ggactggccg
      121 aatcacgtta aagctttcgc gcacggcctt cggcgaacca ggtagattta caatcagaca
      181 gtctgagtag atgccgcaga caccacgcga gatcattgcc atgggagtgt gtttaagcga
      241 cgccgcgcgc attgcttcag ctaagccggg tgcctcacga tcaattacct gacgagttac
      301 ttcaggcgtg ttatcgcgcg ggccgaagcc cgtgccccct gtcgtaataa tcagattcac
      361 gtcgtcgcga ccagcatatt cgcggagtga gtcggccagc gcttcgggat catcagacaa
      421 aacttcatga gcgatgatct cggcgccagc ctcgcgcaac agctccacca atgctgctcc
      481 agaaagatct tcctgctcgc cgttagcaca gcgatcactg actgtgatgg cgacggctat
      541 gatgggagtg gacacaatgg attcagggac aagtttactt agtccgctca cgcggactga
      601 ctttacttcg cggtcagctc ctgcaccaac gaatcagccc cgtagagttt cctgagcgcc
      661 tcaataatcc cggcgctgtg aatggcaatc gcgcggccgc cctcagtttc gtcaacgtac
      721 cagtaacggt tcggcagttt ctgaatgtta ctgtcgaagt ttagtccgac gatttcggct
      781 ttgcggttga taatcgggct accggaattg ccaccaatgg tatccgcggt gtagacaaaa
      841 ttcatcggtg tactgagatc cagtttctgg cgtccatctt tgaaacgctg cggcaaatcg
      901 taaggaactt tctcatcgaa gctcgccgcc cggtcataca agccgctgaa ggtcgttttg
      961 taaggcacca gggttgtatc ttcttcatag ccaagaacac ggccataact aatccgcaat
     1021 tgggagttgg catccggata aacgttgcga ccgtagactg caaaacgagc agtcgcgatt
     1081 ttctgaccgg caacggtttc caggttcaca attttttcct cattccaggt gcggagttcc
     1141 cgaatcaccg gttccactcg acgcgccaaa gcaatcaaag gatcgtcgct cctggcaatc
     1201 gcttcagcgc cgccctcaaa cagtgccttg cgcacggcaa catctcccaa tttcgttcca
     1261 gaaacgacag ctttggcgac ggcagctggc gtggaaccat tcagagcggc gcgcacaaag
     1321 ggatcatttg cgcccagggt tttctcggcc tcgacaaacc acgccgttaa gatcgcctct
     1381 tccatgtctg ggtaaaccgg cgccggcgag aagagtccaa acttaagcga ttcaagtttc
     1441 gaatcgcgaa attcgtcata acgtttgtcg tttggcttcc caatctccac ggaatagcga
     1501 acgagtgagg aagcaatctg gcccaaacgc gaccagacaa gcgttgagaa ggcgctgcgc
     1561 tttgcataag cgggaagctc tccgtaagca gcagcaatct cgtcccacgc tcccccaacc
     1621 gcctgctgca attccggccg acgcgcgact tccgctcgca gagctttctc ttcctcttcc
     1681 ttgcgcgcca tcatgcgcgg attgctcaga ccttcctgct ggccggtgag tcgcttaatg
     1741 gaattcgcca aactgcgaac tgtcgccaat gcacgtcgcg tcgcttccgg cccactggcg
     1801 ccgtaacgca caagggcttc gcgccgtgaa gtccagacca gcatttgtag cggattgcca
     1861 acgtcgcgtt gatacttaag ctgcgacacc gtcagcagcc gattagtgga gccggggcta
     1921 ccgatcgcca acacaaactc gccgtcagcg ggtccggccg ccgaccactt gaagtagttt
     1981 tgcgttttta agggcgctcc gttttcataa actcggaaga aggtgacatc caggcagtag
     2041 cgcgggtaag taaaattatc gtagtcgccg ccaaagaacg ccgcttgctc ctcgggtgcg
     2101 aataccaggc gca
//
LOCUS       DASUEX010000692         2656 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_224598, whole genome shotgun sequence.
ACCESSION   DASUEX010000692 DASUEX010000000
VERSION     DASUEX010000692.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224598"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1259
                     /locus_tag="VFV61_11500"
     CDS             <1..1259
                     /locus_tag="VFV61_11500"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX4901150.1"
                     /translation="GLRVLTVEDHASPTVAIQVWYKVGSKDDPEGRSGFAHLFEHMLF
                     KGTKNMKDEMMDRLTEDVGGINNASTWDDFTNYYEVVPSNYLETLLWAEADRMGSLNV
                     DEKNFKSERDVVKEEFRSRVLAPPYGRLFYAIEKQSFTLHPYRRPGIGSIEELDAATL
                     DDVKAFHSTFYRPDNATLVVVGDFEPKQIDAWIDKYFTRVAKPDRPMPRVTVKEPERT
                     AEKRVNEYAANVPLPAVALTYLAPPVASPDAAALDVAESILSSGESSRLYQSLVYQQQ
                     VAQEAFAQADLREQPGLFILGAILASEKKPEEGERALLAELKKMQDTPVSAAELDKAK
                     LQLVTNELRERETSNGKAFALARAAVLLGDAARVNTDIEKLQAVTAADIQRVMRKYFT
                     DTNRVVITYQAEAARPKATTGGEGSK"
     gene            1256..>2656
                     /locus_tag="VFV61_11505"
     CDS             1256..>2656
                     /locus_tag="VFV61_11505"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX4901151.1"
                     /translation="MRTISNFSKLDFSKLVSIAAVVLATGLPTGTVRAQQENPPAPAA
                     PRSVTIPKPNERTLKNGLRVIVVEDHDIPLVSAGVVVKNGGEVDPESLSGLAELTASL
                     LTKGTKTRTAPEIAQQIEALGGTIESGAGWDASRVFINVMSGKIDPAMSILADVVRNP
                     VFQEEEIERLRQQYIDNLSVTLGQPGSLASFVAARIVFGDAAYGHPLAGTPESLPRIK
                     QADIVGLHAKYYRPDNAVLVIGGSIKAADAFKLAERLFGDWAKPATALPARSEVGKIA
                     RADEKQRVVVVDMPEAGQAAVLLARVGISRTDPDYFRGIVTNSILSGYSGRLNQEIRI
                     KRGLSYGARSSLDVRRESGPFVASAQTKNPSGAEVAGLLISELSRLSSEPVTDAELTP
                     RKAVLIGGFGRNLETTDGLVFQVASLALYGLSLDEINNYIKNVQAVTAADVQKFAGSR
                     LPAKDAHIIIVGNAKDF"
BASE COUNT          609 a          713 c          779 g          555 t
ORIGIN      
        1 acggactgcg ggtgctgact gtcgaagatc atgcgagtcc gaccgtcgcg atccaggtct
       61 ggtacaaagt gggttcaaaa gacgatccgg aaggccgcag cggttttgcg cacctctttg
      121 aacacatgct cttcaagggc acgaagaaca tgaaagacga gatgatggat cgccttacag
      181 aagacgtggg cggcatcaac aacgcttcca cctgggacga tttcacgaac tactacgagg
      241 ttgtgccttc caattatctg gagactctgc tctgggccga agccgaccgg atgggctccc
      301 tcaatgttga tgagaaaaac ttcaagtctg agcgtgacgt cgtcaaagaa gagtttcgct
      361 cgcgcgtgct ggcgccaccg tacggccggt tgttttacgc aatcgagaag caatccttca
      421 ccttgcatcc ttaccgacgc ccgggcattg gcagcatcga ggagcttgac gctgccactc
      481 tcgatgatgt gaaggcgttt cactccactt tttaccgacc cgacaacgcg acgctcgtcg
      541 tcgtcggcga ctttgagcca aaacagatcg atgcctggat cgacaaatac tttacgcgcg
      601 ttgccaaacc cgaccgccca atgccgcggg tgacagtcaa ggaaccagag cgaacggcag
      661 aaaaacgcgt aaacgaatat gcggccaacg tgcccttgcc ggcagttgcg ctcacttacc
      721 tcgcgcctcc tgtcgcttca ccggatgctg cggccctcga tgtagctgaa tcgatccttt
      781 ccagtggcga gtcctcgcgt ttgtatcagt cgctggttta ccagcagcag gtagcgcagg
      841 aagcgttcgc gcaagccgat ctgcgcgagc aacccggtct atttatcctt ggtgcgatac
      901 tcgccagtga aaagaaaccg gaagaagggg agcgggcctt gttggctgaa ctgaaaaaga
      961 tgcaagacac gccggtttcc gctgccgaac tcgacaaggc aaaacttcaa ctggtgacca
     1021 acgagttgcg cgaacgtgag accagtaatg gcaaggcttt cgccctcgca cgggccgccg
     1081 tgttactcgg ggatgcagca cgggtaaaca ccgatattga gaaacttcaa gccgttacag
     1141 ccgcggatat tcaacgcgtg atgaggaagt atttcactga cactaaccgg gtggtgatta
     1201 cttatcaggc agaagcggcg cggccgaaag caaccaccgg cggggagggg agcaaatgag
     1261 aacgatttcc aacttttcaa aattagactt ttcaaaatta gttagtatcg cggcggtagt
     1321 gttagcaacc ggcttgccta ccgggacggt gcgcgcgcaa caagagaatc cgccggcgcc
     1381 ggccgcgccg cgctcagtga ccatacccaa accaaacgag cgcacgctga agaatggtct
     1441 gcgcgtgatc gtggtcgaag atcacgacat tcccctagtt tccgccggag tggtggtgaa
     1501 gaacggcggc gaagtcgatc cggaaagctt gagcggtttg gcggagctaa cagcttcatt
     1561 gcttaccaag ggaacgaaga cgcgcacggc gccggagatt gctcaacaaa ttgaagccct
     1621 gggcggcaca atcgagtcgg gtgccggttg ggacgcgtca cgcgttttca tcaatgtcat
     1681 gtccggcaag atcgatccgg cgatgagtat tctggcggac gttgtgcgaa atccagtctt
     1741 tcaggaagaa gagattgagc gtctgcgcca acagtacatc gataacctga gtgtcacgtt
     1801 aggccagccc ggatccctgg ccagcttcgt ggcggcgcgc attgtgtttg gtgatgctgc
     1861 ttatggtcat ccgcttgcgg gcacgcccga atcgttgccg cgcattaagc aggccgacat
     1921 cgtcggactt catgcgaaat attaccggcc tgacaacgct gtgctcgtga taggcggttc
     1981 gatcaaagct gcggatgctt tcaagctggc agaacgtttg ttcggcgatt gggccaagcc
     2041 ggcgaccgcc ctgcccgcgc gcagcgaggt tggtaagatc gcgcgcgccg atgaaaaaca
     2101 acgcgtagtc gtcgttgaca tgccggaagc gggccaggca gctgtgctgc tggcgcgggt
     2161 tggcattagt cgcaccgacc cagattactt tcgcggcatt gttacgaatt caatcctctc
     2221 cggctattcc ggaagattga atcaggaaat tcgcatcaag cgcggactta gttatggcgc
     2281 gcgcagctcg ctcgacgtgc ggcgcgaatc agggcccttt gtggcttcgg cgcaaactaa
     2341 gaatccctct ggagccgaag tcgccgggct gttgattagc gaactcagtc gcttgtcgag
     2401 tgaacctgtt accgatgctg agttgactcc gcggaaggct gtattgattg gaggctttgg
     2461 tcgcaacctg gaaacaacgg atggactggt gtttcaggta gcttcgttag cgctatatgg
     2521 tctcagtctg gatgagatca ataactacat taaaaacgtc caggcagtaa ctgccgccga
     2581 cgtgcagaag tttgccggtt cgcgtttgcc ggcaaaggat gcgcacatca tcatcgtcgg
     2641 caatgcgaaa gacttt
//
LOCUS       DASUEX010000693         3755 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_226057, whole genome shotgun sequence.
ACCESSION   DASUEX010000693 DASUEX010000000
VERSION     DASUEX010000693.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3755
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226057"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..1055)
                     /locus_tag="VFV61_11510"
     CDS             complement(93..1055)
                     /locus_tag="VFV61_11510"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX4901152.1"
                     /translation="MKLKKRYLLAGFSGLATAAVVTKLLARPRDVDLERSRDRIYHVE
                     HSRFIDVNGLRVHYQEAGDPAAPPLFLIHGFLSSTFVWSRVFLGLAAAGFRVIAPDLI
                     GYGYSAKPRNFDYSIESQAKMLAGLLERLGSNGATLVGSSYGGAVAATVALDQPELVR
                     KLVLVGAVSNNEPKRFMMMRLVRTPVVGDIVSPLLAGSRRLLRARMKRVYDRHQASLD
                     EFRVDVRFLPLRAAGTHRAIIRTVRNWDAERIQREARLIKQPTLLIWGDNDPDVPLRD
                     GEELQQQIESSRLLVFRDCGHHPQEEYPTSFTEAVLEFCDKPLT"
     gene            complement(1064..1984)
                     /gene="truB"
                     /locus_tag="VFV61_11515"
     CDS             complement(1064..1984)
                     /gene="truB"
                     /locus_tag="VFV61_11515"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEX4901153.1"
                     /translation="MDGVLIIDKPAGMTSHDVVARVRRIVGERRVGHTGTLDPFATGI
                     LVVLVGRATRLAQFLGGSVKEYEAVIRLGFSTDTGDVTGKRLTPVQEKPNPWTNEEIQ
                     DALASLRGEIEQVPPMYSAKKIAGRKLYELARRGEEVERKPVRVTIHSLELAGNVGKI
                     FPQNVDGTTDIQIRVTCSAGTYVRTLAEDVGKRLGVGAHVVALRRTRAGNFDLKRAKT
                     LEQLEESMRQGSLDDILVSPDLAIEFLPSVSLTSLEVQRTQQGLPLKLGSKQALREAD
                     WVRMRDAAGVLVAVGTYDLEAELVRPRVVL"
     gene            complement(2003..2902)
                     /locus_tag="VFV61_11520"
     CDS             complement(2003..2902)
                     /locus_tag="VFV61_11520"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX4901154.1"
                     /translation="MPNTPAKPASSPLLLVLAAAIIWSTGGLFIKWTTLSGLELSFGR
                     SLLAAITVALFTRREGFGLNRVTLIASVLYAALLLLFVLATKETTAANAIFLQYTAPL
                     YVLVLEPIFYKEKFRQRDLITVIFCVIGMSLFFVGKLRPQDVTGNLLALASGVCFACY
                     FLLLRHSSARTVNRASSVIYGNLLVVLVAAPAGLKALSGVTMHDALSVVYLGVVQIGI
                     AYTLFTLGMARGIRSLDAGIVGYIEPVLNPVWVFLVIGERPSQWALIGGVIIVGAVVA
                     HTTLKAKEGRKAIILTGPESGAK"
     gene            complement(2927..>3755)
                     /locus_tag="VFV61_11525"
     CDS             complement(2927..>3755)
                     /locus_tag="VFV61_11525"
                     /inference="COORDINATES: protein motif:HMM:NF012339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX4901155.1"
                     /translation="AEDSQQLARELLLQGDYLLKDQIDTSHRFLYGHRYWPQVKQAIA
                     EHAASFTETNDKPLTDQIIDVASQVAASLRIDPSLTTGITAAGFMTVQQVGVSAFNAD
                     SGKMTLDSKHAKLSPEQVLKERAKDDSQGLLGFLKTVDKKWTVTHDENDDAAKYKLNN
                     AQDLAWGAEADRSRDWRALDPRRIEGPIPVECRSASCGTCWVGVLGGAEKLSDVAARE
                     GKQIKLFGYINTTEPKPLIRLACQAQAQGAVSVVIPPWNGVFGKYLQRRNHRVADDE"
BASE COUNT          845 a         1078 c         1012 g          820 t
ORIGIN      
        1 tcggactctc attgagacaa ccgatcaggt tattcgttct tctctcggcg aatttggcga
       61 ggcttggcct cttggctgtt cgatggctcg atctaagtaa gaggcttgtc gcagaactcc
      121 agaacggctt cggtaaacga agttggatac tcttcctgcg gatgatgacc gcagtctcta
      181 aaaactaaca gtcgcgaact ctcgatctgc tgctgtagtt cctcgccatc tcttaaaggc
      241 acatcgggat cgttatcacc ccaaatcagc agtgtgggtt gtttgatcaa gcgcgcctcg
      301 cgctggattc gctctgcatc ccaattgcgt acggtacgaa tgattgcccg gtgagtgcct
      361 gccgctcgca gtggcaggaa gcgaacgtca acacgaaatt cgtcgagaga tgcttgatgg
      421 cgatcgtaaa ctcgcttcat gcgcgctcgc aataagcgcc gcgacccggc cagcaagggc
      481 gaaacgatgt caccgacgac cggcgtccgc accagtcgca tcatcatgaa gcgcttgggt
      541 tcgttgttgg agaccgcacc aactaagacc aacttccgaa cgagctcagg ttgatctaat
      601 gcaaccgttg ccgcgactgc gccgccgtaa gagctgccca ccaaagtggc gccgttgctt
      661 cctaatcgtt ccagcagtcc ggccagcatc tttgcctgcg actcgattga atagtcaaaa
      721 tttcgtggtt tagctgaata gccgtagcca atcaaatcgg gcgcgatgac gcgaaaaccg
      781 gcagctgcta gtcccaaaaa cacgcggctc cacacaaagg tcgacgagag aaagccatga
      841 atgagaaaca gcggtggcgc ggcagggtcg ccggcttcct ggtaatgtac gcgcaagcca
      901 ttgacgtcga tgaaacgaga atgttcgacg tggtagatgc ggtcgcgact tcgttccaga
      961 tctacgtcgc gaggccgcgc caacaatttc gtcaccacag cagccgttgc caagccggaa
     1021 aatccggcaa gtagatagcg tttcttcagt ttcattgttc cagttacagc acgacgcgtg
     1081 gccgcaccag ttcggcttcg agatcgtatg tcccgaccgc gaccagcacg ccggcagcat
     1141 cgcgcatccg tacccagtcg gcctcgcgga gggcttgttt gcttcccagc ttcaagggta
     1201 gaccttgttg agttcgctgc acttccagcg aagtgagtga cacgcttggc agaaactcaa
     1261 tggcgaggtc aggtgaaacc aggatgtcgt ccaatgatcc ctggcgcatg ctctcttcca
     1321 gttgctccag tgttttcgcg cgcttcagat caaaattgcc agctctcgtt cgtcgcagtg
     1381 cgactacatg ggccccgact cccaggcgtt ttccgacatc ctcagcgaga gtgcgcacgt
     1441 atgttcccgc agaacatgtg actcggatct gaatgtcagt ggtgccgtca acgttctgcg
     1501 ggaatatttt gcccacgttc ccagcgagtt ccaaagagtg aatcgttacg cggacaggtt
     1561 ttcgttcaac ttcttcgccc cgccgagcga gttcatagag cttcctgcct gcgattttct
     1621 ttgccgaata catcggcggc acttgctcaa tctctccccg caaactcgcc agcgcatcct
     1681 ggatttcctc gttagtccag ggattcggtt tctcctgaac tggagtgaga cgctttccgg
     1741 tcacatcccc ggtgtcggta ctgaacccca accgaatcac ggcttcatat tctttaacag
     1801 agccgccgag gaattgcgcc agtcgcgtcg ccctgccgac gagaacgacc aagatgccgg
     1861 tggcgaacgg atcaagcgtt cccgtgtgtc ctacacgtcg ttcgcctacg attcgtcgca
     1921 cacgagcaac cacatcgtgc gatgtcatgc ccgcaggctt atcgattatc aagactccat
     1981 ccatctcagc tattgaggat gatcacttgg caccggactc aggcccggta agaataatag
     2041 ccttgcgccc ttcttttgcc ttcaacgttg tatgagccac cacggcgccc acaatgatca
     2101 cgccgccgat gagagcccac tgggaaggtc gctcgccgat aaccagaaaa acccagacag
     2161 ggtttagcac cggctcgata taaccgacga tgccggcatc gagcgagcga atgccgcgcg
     2221 ccatgccgag cgtgaaaagc gtgtaagcaa ttccgatttg cactactcca agatagacga
     2281 cgctcagcgc gtcgtgcatg gtgacgcctg acaatgcctt caatcctgcg ggagcggcga
     2341 ccaacacaac cagcaggttg ccgtagataa ccgacgacgc ccgatttacg gtccgcgcgc
     2401 tggagtgccg cagcaatagg aagtagcacg caaaacagac tcccgacgcg agcgctagta
     2461 ggtttccggt gacgtcctga ggccggagct tgcccacaaa gaaaagcgac atcccgatca
     2521 cacaaaagat cactgtgatt agatcccgtt ggcgaaactt ctccttgtaa aagatcggtt
     2581 ccagcaccag gacgtaaagc ggtgcggtgt attgcaggaa aatcgcgttg gccgcagttg
     2641 tttccttggt tgccagtacg aaaagcaaaa gcagcgctgc gtaaagcact gaggcgatca
     2701 aggtaacgcg attcaagcca aaaccttcgc gtcgcgtaaa taacgcgact gtgatcgcag
     2761 ccagcagcga acgaccgaaa gagagctcca ggcccgagag cgtagtccac tttatgaaga
     2821 gaccgccggt actccagatg atagccgcag ccagaaccag caataatggc gaggacgcag
     2881 gtttggcggg agtgttaggc aagctactaa aagacgtgtt cagtttctac tcatcgtctg
     2941 cgacgcgatg attgcggcgt tgcaagtatt ttccgaagac gccattccag gggggaataa
     3001 caaccgagac agctccctgc gcctgggcct ggcatgcgag gcgaatcagc ggcttgggtt
     3061 cggtcgtgtt tatgtaaccg aagagtttaa tctgcttgcc ttcgcgggcg gcaacatcgg
     3121 aaagcttttc cgcgccgcca agcacgccta cccaacaagt accacacgac gccgagcggc
     3181 attcgacggg gattggtccc tcgatgcgtc ggggatcgag cgcccgccaa tcgcgtgacc
     3241 gatctgcctc tgcgccccag gccagatctt gcgcattgtt gagtttgtat ttcgccgcat
     3301 catcattttc gtcgtgagta acggtccatt tcttgtctac cgttttcagg aaccctaata
     3361 agccctggct gtcatccttc gctcgctctt tgagaacctg ttcgggcgac agtttcgcgt
     3421 gcttgctatc gagcgtcatt ttccccgagt cagcgttaaa ggctgagacg cctacttgct
     3481 gaactgtcat aaaaccagcc gccgtgatgc ccgtggtgag agatggatcg atgcgcagac
     3541 tcgctgcaac ctgggaagcc acgtcaataa tctgatccgt caacggctta tcattagttt
     3601 cagtaaaact ggcagcatgt tcggcgatgg cttgtttcac ttgcggccag taacgatgcc
     3661 cgtagaggaa gcgatgcgaa gtgtcgatct gatcttttag caagtagtcg ccctggagca
     3721 gcagttcgcg tgcgagttgc tgcgagtctt ccgcc
//
LOCUS       DASUEX010000694         2443 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U1364
            k141_227407, whole genome shotgun sequence.
ACCESSION   DASUEX010000694 DASUEX010000000
VERSION     DASUEX010000694.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961516
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,287
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227407"
                     /isolate="SMAG_U1364"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1888)
                     /locus_tag="VFV61_11530"
     CDS             complement(<1..1888)
                     /locus_tag="VFV61_11530"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX4901156.1"
                     /translation="MTPERWQEIKQLFNAALEHEPAGRLAFLAQASGNDESLRQEVAS
                     LLESLEQATDFIETPASDLAAELLARGQTGLVSGQMVGPFRISDVIAAGGMGEVYLAD
                     DTRLGRKVALKLLPPQFTVNADRVRRFELEARAASALNHPNIVTIHEIGETDDLHFIA
                     TEFVAGQTLREHMRKTRMTVGEVLDIGVQIASALQAAHDAGIVHRDIKPENIMLRHDG
                     FVKVLDFGLAKLAAPLNSISETPETLLLTNPGVVMGTVAYMSPEQARALEVDARADIW
                     SLGVVLYEMVAGRAPFEEPTSSDVIAAILKDEPESLATRADVPAELAQIIAKALRKDR
                     TLRYQSAGELASDLKNLKEELGVTERLNRRFDPGKRSALSGKAGAEHDESGHQAVTTG
                     PIPQPLTTVSVEYLVGNIQRHKILISVALLVLLGIGLTYIRFSRNKANSPTSDRSIAV
                     LPLKPVDPANRDDLYEIGITDSLIRQLGSMKGIFVRPLSATRQYRDIAQDPIAAGKEQ
                     QVDYVLATNYQLTGGRISITAQLLNVASGQIEESYKTEKDLANNFAMQDAVAVEIGNW
                     LLVQFATTTNRLARKRGTSNEEAYRLFLQGMYLANNRNLEDARRAIEALEKAVQLDPN
                     YARAW"
     gene            complement(1997..>2443)
                     /locus_tag="VFV61_11535"
     CDS             complement(1997..>2443)
                     /locus_tag="VFV61_11535"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HEX4901157.1"
                     /translation="QYMRREREAHTLQTSALLNEAYIRLVDQKGIQWRDRAHFFGIAA
                     RLMRQVLVDYARRRNYAKRGGDLRHVPLDEALIVSRERAAEVLALDDALKTLAEIDSR
                     QSEIVELRFFGGLSIEETAEVLAVSPGTVMREWTLAKAWLRRSITS"
BASE COUNT          510 a          699 c          657 g          577 t
ORIGIN      
        1 cccatgcccg cgcgtaattc gggtcaagct gaaccgcttt ttccaaagcc tcgatggctc
       61 tccgcgcgtc ctccagattt ctattgttgg ccaggtacat tccctgtaga aacagccgat
      121 acgcctcctc gttcgacgtg ccgcgtttgc gcgcgagacg gttcgtggtg gtggcaaact
      181 gcaccagcag ccagttaccg atctcgactg caacggcatc ctgcatggcg aagttgttcg
      241 caagatcctt ttcggtttta tagctctctt caatctgtcc ggatgcgaca ttcaaaagct
      301 gtgcagtgat gctaattctt ccaccggtca actgatagtt cgtcgccaga acgtagtcca
      361 cctgttgttc cttgcccgcg gcaattgggt cttgcgcgat atcacgatac tggcgagtag
      421 cgcttaacgg acggacgaaa attcctttca tcgacccgag ctgccgaatc agcgagtccg
      481 taataccgat ctcgtagagg tcgtcgcgat tcgctgggtc gaccggtttc agtggtaaga
      541 cggctatgga tctatcgctg gttggtgagt tcgctttgtt ccgactgaac cgaatgtacg
      601 tcaggccaat tccgagtagg acgagcaaag ccacactaat gagaatcttg tgacgttgaa
      661 tgttgccaac aagatactca acactgactg tcgtaagcgg ctgcggaata ggtccagtgg
      721 tcaccgcttg gtggccactc tcgtcatgtt ctgcgccagc cttgccgctt agggcagacc
      781 tttttcctgg gtcgaatctt cggtttagcc gttccgtcac gccgagttct tccttcaagt
      841 tcttcaaatc tgacgcgagc tcgccggcgg attgataacg tagagtgcga tcctttcgga
      901 gcgctttggc gatgatttgc gcaagttccg caggtacgtc tgcccgagtt gccagtgact
      961 cgggttcatc cttgaggatg gccgcaataa catcactcga cgtaggttcc tcaaacggcg
     1021 ctcgtcccgc gaccatctcg taaagcacaa cacccaaact ccaaatgtcc gcccgcgcat
     1081 cgacctcaag cgcccgcgcc tgctcgggtg acatgtatgc aaccgtcccc atcactacgc
     1141 ccggattcgt cagcagcagt gtctcagggg tttcactaat cgagttgagt ggtgcggcaa
     1201 gctttgcgag cccgaagtcc aggaccttca cgaagccatc gtgacgcagc atgatgtttt
     1261 cgggtttgat gtcgcggtga actatccccg catcatgcgc ggcctgaagt gcggacgcga
     1321 tctgaactcc gatatcgagc acttcaccga cagtcatccg cgtcttcctc atgtgctcgc
     1381 ggagtgtctg gcctgcgaca aattcagtgg caataaaatg aagatcgtcg gtctctccaa
     1441 tctcgtggat ggtgacgata tttgggtgat tcagagctga cgccgcgcgt gcctctagct
     1501 cgaaccggcg caccctgtct gcgttcacag tgaattgggg tggcaggagc ttgagtgcta
     1561 cttttcggcc aagccttgtg tcgtcagcca ggtaaacttc tcccattcct cccgccgcga
     1621 ttacgtctga gatgcggaat ggtccaacca tctgaccaga cacgagacca gtctgaccac
     1681 gtgcaagcaa ctctgctgca aggtctgaag ctggcgtctc gatgaagtcg gtggcttgct
     1741 cgagggactc taaaagtgag gcaacctcct gacggagaga ctcgtcgttt ccgctcgctt
     1801 gcgcgaggaa ggccagccgg ccggcaggct catgctccag ggccgcatta aaaagctgtt
     1861 tgatctcttg ccagcgttcg ggcgtcatgg caaccggtaa ttttgatgag gcaatatgaa
     1921 agaaactctt gggaatccat tcactgcctt tccaacttca aaatgcgagg ggaacggcaa
     1981 aaacctctca ctgaggtcag gacgttatgc ttctgcggag ccaggctttg gcgagggtcc
     2041 attcgcgcat gaccgtgccg ggtgaaacgg cgaggacttc ggccgtctcc tcaatactca
     2101 agccgccgaa aaaccgaagt tcgacgattt cactttggcg actatctatc tcagcaagag
     2161 tcttgagagc atcgtccagc gccaggactt ctgcggcgcg ctccctagag acaatcaacg
     2221 cctcatcgag cggcacatgg cgtaaatctc cgccgcgttt cgcgtaattt cgccttcgcg
     2281 catagtcaac cagaacttgc cgcattagcc gggctgcgat gccgaagaag tgggcccgat
     2341 cccgccactg aatgcctttc tgatcgacca ggcgaatata ggcttcatta agaagcgcag
     2401 aggtctgcag tgtgtgagcc tcacgttcac gcctcatgta ctg
//
